{"data":[{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":1,"idioma_id":4,"titulo":"Sedative effect of central administration of Coriandrum sativum essential oil and its major component linalool in neonatal chicks","resumen":"ContextCoriandrum sativum L. (Apiaceae) (coriander) is an herb grown throughout the world as a culinary, medicinal or essential crop. In traditional medicine, it is used for the relief of anxiety and insomnia. Systemic hydro-alcoholic and aqueous extract from aerial parts and seeds had anxiolytic and sedative action in rodents, but little is known about its central effect in chicks. Objective To study the effects of intracerebroventricular administration of essential oil from coriander seeds and its major component linalool on locomotor activity and emotionality of neonatal chicks. Materials and methods The chemical composition of coriander essential oil was determined by a gas-chromatographic analysis (> 80% linalool). Behavioural effects of central administration of coriander oil and linalool (both at doses of 0.86, 8.6 and 86 &#956;g\/chick) versus saline and a sedative diazepam dose (17.5 &#956;g\/chick, standard drug) in an open field test for 10 min were observed. Results Doses of 8.6 and 86 &#956;g from coriander oil and linalool significantly decreased (p < 0.05) squares crossed number, attempted escapes, defecation number and distress calls, and significantly increased (p < 0.05) the sleeping posture on an open field compared with saline and were similar to the diazepam group. Discussion and conclusion The results indicate that intracerebroventricular injection of essential oil from Coriandrum sativum seeds induced a sedative effect at 8.6 and 86 &#956;g doses. This effect may be due to monoterpene linalool, which also induced a similar sedative effect, and, therefore, could be considered as a potential therapeutic agent similar to diazepam.","palabras_clave":"coriandro|sedacion|aceite esencial"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5,"idioma_id":4,"titulo":"INTRA-SPECIFIC PELAGE COLOR VARIATION IN A SOUTH AMERICAN SMALL RODENT SPECIES","resumen":"Intra-specific color variation is often underestimated by researchers, and among mammalian species, intra-specific differences in coloration are poorly documented for most species. The main goal of this study was to apply an objective color measurement methodology to the study of a specific problem: the detection, if any, of patterns of changes in the fur color of specimens of Akodon budini in relation to biological (i.e., sex) and environmental (i.e., season) variables. We hypothesize that male coat color will be more homogeneous than that of females and that the winter coat color will be darker than that of summer, the latter being orange. We measured the pelage color on five points over the dorsal surface of 26 A. budini museum specimens region using a spectroradiometer and a diffuse illumination cabin. We used Principal Component Analysis to describe the association between the color variables, sex and season, and each of the observations. We then used general linear models of Analysis of Variance to examine relationships between color data, season, and sex. The results clearly confirm the hypothesis related to seasonal coat color change but do not directly confirm the hypothesis related to changes in coat color in relation to sex, and we show the complexity of the studied pattern. In conclusion, undoubtedly, the studied variables should accordingly be taken into account when studying the coloration of specimens for characterization, identification and discrimination of different taxonomic units based on color.","palabras_clave":"COLECCIONES|AKODON|ARGENTINA|COLOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6,"idioma_id":5,"titulo":"OBSIDIANAS EN EL TARDÍO-INKA DE ANTOFAGASTA DE LA SIERRA (PUNA MERIDIONAL ARGENTINA): ¿FONDO DE CUENCA VERSUS SECTORES INTERMEDIOS?","resumen":"Este trabajo busca evaluar la circulación de distintas variedades de obsidiana en la microrregión de Antofagasta de la Sierra con el objetivo de discutir aspectos de la organización político-social de las sociedades que la habitaron durante el Tardío-Inka. Para ello se recurrió a análisis geoquímicos sobre muestras de obsidiana procedentes de sitios arqueológicos emplazados en distintos microambientes de la cuenca, fondo de cuenca y sectores intermedios. Los resultados alcanzados llevan a sugerir que las unidades familiares pastoriles de los últimos sectores habrían accedido irrestrictamente a la circulación de esta roca, sin mediar el control de las elites emplazadas en el fondo de cuenca. Esto conforma una evidencia más en pos de reconocer el poder social de estos grupos en el entramado político-social de la cuenca con posterioridad a ca. 1100 años AP.","palabras_clave":"XRF|Tardío-Inka|unidades familiares pastoriles|elites|poder social"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":8,"idioma_id":4,"titulo":"Antioxidant status, lipid and color stability of aged beef from grazing steers supplemented with corn grain and increasing levels of flaxseed","resumen":"Angus steers were grazed on unsupplemented pasture (CNTRL), pasture supplemented with 0.7% BW cracked corn (FLAX-0), FLAX-0 with 0.125% and 0.250% BW of whole flaxseed (FLAX-1 and FLAX-2). Six steers were grazed per treatment for 70 days, with start and finish weights of 458 and 508 kg. At 24 h post slaughter, longissimus thoracis were harvested, and steaks assigned to treatments of postmortem aging time under vacuum (PM; 3, 14 and 56 days) with or without five days of aerobic exposure (AE). Meat antioxidant status was higher (P < 0.05) when feeding CNTRL and FLAX-1 than FLAX-0 and FLAX-2. Under AE, lipid oxidation was highest for FLAX-2 (P < 0.05), and lowest for FLAX-1. Greatest TBARs and lowest antioxidant capacity and redness values were obtained with AE and the longer PM (P < 0.05). Beef oxidative stability through AE improved by adding a low flaxseed level to supplemented corn grain, but deteriorated by adding a high flaxseed level or by extending PM.","palabras_clave":"Argentine beef|Oxidative stability|Aging|Retail display|Antioxidant|Pasture|Oil seeds"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":12,"idioma_id":4,"titulo":"Effect of high-pressure treatment on hard cheese proteolysis","resumen":"The application of high hydrostatic pressure (HHP) treatment has been proposed to reduce the ripening time of cheese via modifications in the enzymatic activities or the substrate reactivity. Investigations on the effect of HHP on cheese proteolysis have been undertaken with either encouraging results or little effect according to the treatment conditions and the type of cheese, but information concerning the effect of HHP on the ripening of hard cooked cheese is still lacking. In this report, we describe the effect of HHP treatment on Reggianito cheese proteolysis. For that purpose, 1-d-old miniature cheeses (5.5-cm diameter and 6-cm height) were treated at 100 or 400&#8197;MPa and 20°C for 5 or 10&#8197;min, and control cheeses in the trial were not pressurized. All cheeses were ripened at 12°C during 90&#8197;d. The HHP did not affect gross composition of the cheeses, but microbial load changed, especially because the starter culture count was significantly lower at the beginning of the ripening of the cheeses treated at 400&#8197;MPa than in controls and cheeses treated at 100&#8197;MPa. Cheeses treated at 400&#8197;MPa for 10&#8197;min had significantly higher plasmin activity than did the others; the residual coagulant activity was not affected by HHP. Proteolysis assessment showed that most severe treatments (400&#8197;MPa) also resulted in cheeses with increased breakdown of &#945;S1- and &#946;-CN. In addition, nitrogen content in soluble fractions was significantly higher in cheeses treated at 400&#8197;MPa, as well as soluble peptides and free AA production. Peptide profiles and individual and total content of free AA in 60-d-old treated cheese were as high as in fully ripened control cheeses (90&#8197;d). Holding time had an effect only on pH-4.6-soluble nitrogen fraction and plasmin activity; cheese treated for 10&#8197;min showed higher values than those treated for 5&#8197;min, at both levels of pressure assayed. We concluded that HHP treatments at 400&#8197;MPa applied 1&#8197;d after cheesemaking increased the rate of proteolysis, leading to an acceleration of the ripening process in Reggianito Argentino cheese, whereas 100-MPa treatments did not lead to significant changes.","palabras_clave":"HIGH HYDROSTATIC PRESSURE|REGGIANITO CHEESE|RIPENING ACCELARATION|PROTEOLYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":16,"idioma_id":4,"titulo":"Synthetic stigmastanes with dual antiherpetic and immunomodulating activities inhibit ERK and Akt signaling pathways without binding to glucocorticoid receptors","resumen":"Background We have previously shown that some synthetic hydroxylated stigmastanes derived from plant sterols inhibit in vitro HSV-1 replication in ocular cell lines and decrease cytokine production in stimulated macrophages, suggesting that these steroids might combine antiviral and immunomodulating properties. In this paper we report the synthesis of some analogs fluorinated at C-6 in order to study the effect of this modification on bioactivity. Methods The following methods were used: organic synthesis of fluorinated analogs, cytotoxicity determination with MTT assays, cytokine production quantification with ELISAs, glucocorticoid activity determination by displacement assays, immunofluorescence and transcriptional activity assays, studies of the activation of signaling pathways by Western blot, antiviral activity evaluation through virus yield reduction assays. Results We report the chemical synthesis of new fluorinated stigmastanes and show that this family of steroidal compounds exerts its immunomodulating activity by inhibiting ERK and Akt signaling pathways, but do not act as glucocorticoids. We also demonstrate that fluorination enhances the antiviral activity. Conclusions Fluorination on C-6 did not enhance the anti-inflammatory effect, however, an increase in the in vitro antiviral activity was observed. Thus, our results suggest that it is possible to introduce chemical modifications on the parent steroids in order to selectively modulate one of the effects. General significance This family of steroids could allow the development of an alternative treatment for ocular immunopathologies triggered by HSV-1, without the undesirable side effects of the currently used drugs.","palabras_clave":"SYNTHETIC STEROIDS|FLUORINATED STEROIDS|ANTIHERPETIC|CYTOKINES|SIGNALING PATHWAYS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":18,"idioma_id":4,"titulo":"Electronic properties in intrinsically disordered double perovskites: Sr3MnMo2O9 and Ba3MnMo2O9 with Mo5+ valence state","resumen":"Sr3MnMo2O9 and Ba3MnMo2O9 double perovskites with Mo5+ valence states have been prepared in polycrystalline form by thermal treatment, in reducing atmosphere (H2\/N2 flow), of previously decomposed citrate precursors. These materials have been studied by X-ray and neutron powder diffraction (XRPD, NPD), magnetic and transport measurements. At room temperature, the crystal structure of Sr3MnMo2O9 is monoclinic, space group P21\/n, with a = 5.6564(1) Å, b = 5.6383(1) Å, c = 7.9765(2) Å, &#946; = 89.994(7)º; whereas Ba3MnMo2O9 presents a cubic lattice, space group Fm 3m, with a = 8.14900(8) Å. These samples present a frustrated magnetic interaction below to 12 and 9 K for Sr3MnMo2O9 and Ba3MnMo2O9, respectively. Semiconductor behaviour, observed either from d.c. or a.c. measurements, was modelled by a variable-range hopping mechanism. This transport behaviour is in accord with the electronic configurations Mn2+(3d5)?Mo5+(4d1) and supports the absence of mixed valence states in both double perovkites.","palabras_clave":"Inorganic materials|Crystal structure|Electronic properties|Magnetic measurements|Neutron diffraction|Impedance spectroscop"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":20,"idioma_id":4,"titulo":"Photo -Fries rearrangement of aryl acetamides: regioselectivity induced by the aqueous micellar green environment","resumen":"Photochemical reactions tend to give more than one photoproduct. However, such a reaction can be apowerful synthetic tool when it is possible to conduct it in regioselective conditions yielding a singlephotoproduct. Water?surfactant solutions as reaction media can be considered as an approach in thiscontext because they show products with different features than those from isotropic solutions. Here wedescribe results obtained from studying the effect on the prototypical photoreaction, known as thephoto-Fries reaction of several substituted acetanilides and á-naphthyl acetamide within surfactantmicelles (ionic and non-ionic micelles). This reaction involves homolytic cleavage of a C?N bond to yielda singlet radical pair. The surfactant micelles control the rotational and translational mobility of the radicalpair, resulting in noticeable photoproduct selectivity.","palabras_clave":"Photo-Fries|micellas|reordenamiento|aectanilides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":27,"idioma_id":4,"titulo":"Intraspecific variation in essential oil composition of the medicinal plant Lippia integrifolia (Verbenaceae). Evidence for five chemotypes","resumen":"The aerial parts of Lippia integrifolia (incayuyo) are widely used in northwestern and central Argentina fortheir medicinal and aromatic properties. The essential oil composition of thirty-one wild populations ofL. integrifolia covering most of its natural range was analyzed by GC and GC?MS. A total of one hundredand fifty two terpenoids were identified in the essential oils. Sesquiterpenoids were the dominant componentsin all but one of the collections analyzed, the only exception being a sample collected in San Juanprovince where monoterpenoids amounted to 51%. Five clearly defined chemotypes were observed. Onepossessed an exquisite and delicate sweet aroma with trans-davanone as dominant component (usuallyabove 80%). Another with an exotic floral odour was rich in oxygenated sesquiterpenoids based on therare lippifoliane and africanane skeletons. The trans-davanone chemotype is the first report of anessential oil containing that sesquiterpene ketone as the main constituent. The absolute configurationof trans-davanone from L. integrifolia was established as 6S, 7S, 10S, the enantiomer of trans-davanonefrom ?davana oil? (Artemisia pallens). Wild plants belonging to trans-davanone and lippifolienone chemotypeswere propagated and cultivated in the same parcel of land in Santa Maria, Catamarca. The essentialoil compositions of the cultivated plants were essentially identical to the original plants in the wild,indicating that the essential oil composition is largely under genetic control. Specimens collected nearthe Bolivian border that initially were identified as L. boliviana Rusby yielded an essential oil practicallyidentical to the trans-davanone chemotype of L. integrifolia supporting the recent view that L. integrifolia(Gris.) Hieron. and L. boliviana Rusby are synonymous.","palabras_clave":"INCAYUYO|LIPPIA INTEGRIFOLIA|VERBENACEAE|ABSOLUTE CONFIGURATION|BIOGENESIS|CHEMOTYPES|ESSENTIAL OIL|MONOTERPENE|SESQUITERPENE|TRANS-DAVANONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":32,"idioma_id":4,"titulo":"Vibrational Spectra of Barium Oxalate Hemihydrate","resumen":"The infrared and Raman spectra of barium oxalate hemihydrate, BaC2O4 · 0.5H2O, were recorded and discussed on the basis of their structural peculiarities and in comparison with the spectra of the previously investigated calcium and strontium oxalates.","palabras_clave":"Barium oxalate|Infrared spectra|Raman spectra"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":34,"idioma_id":4,"titulo":"Relationship between endothelial progenitor cells and vascular endothelial growth factor and its variation with exercise","resumen":"physical activity and a single exercise test on the number of CD309+ circulatingendothelial progenitor cell (EPC) and their relation to the variation in plasmalevels of VEGF in chronic coronary patients.Methods: 21 patients <75 years with chronic stable coronary artery diseasewere included. All patients underwent exercise myocardial perfusion SPECT.Then, participants were divided into two groups: one group (11 patients)underwent cardiac rehabilitation program and the other (10 patients) continuedwith the standard treatment. Blood samples were obtained at baseline, 30minutes after exercise ended and at one and three months during follow-up.Results: VEGF values decreased significantly after exercise SPECT test. Afterone month, there was a significant increase in VEGF levels compared to thosemeasured immediately after exercise. All patients showed a decrease in thevalues of EPC at 1 and 3-month follow-up. There was an inverse andstatistically significant relation between change of EPC and VEGF between thebaseline and 1 month.Conclusions: The increase of VEGF at 1-month, with respect to baselinevalues correlated with decreased levels of EPC. This association wasindependent of the onset of ischemia in the perfusion study.","palabras_clave":"CORONARY ARTERY DISEASE|EXCERCISE|VEGF LEVELS|PROGENITOR CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":36,"idioma_id":4,"titulo":"Monte Carlo Simulations of Settlement Dynamics in GPUs","resumen":"Recently, a Monte Carlo model was proposed in order to simulate settlement dynamics in drylands, including several environmental factors, and it was implemented as a serial CPU code. In this work we present a parallel implementation of that code using graphics processing units (GPU) and NVIDIA CUDA. The code was tested with two experiments, a Baseline case and a Realistic case. We take advantage of the GPU architecture to obtain significant speedups: &#8764;&#8764;8×× to &#8764;&#8764;20×× with the Baseline case in a NVIDIA Tesla C2050 versus a Phenom 1055T CPU. The Realistic case obtained &#8764;&#8764;80×× of speedup in the same hardware. The GPU performance of the code will allow the inclusion of additional factors affecting settlements and large grid sizes for detailed environmental degradation models.","palabras_clave":"Monte Carlo |Parallel processing |General purpose graphics processing units|Settlement dynamics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":42,"idioma_id":4,"titulo":"Potential of Pseudomonas putida PCI2 for the protection of tomato plants against fungal pathogens","resumen":"Tomato is one of the most economically attractive vegetable crops due to its high yields. Diseases cause significant losses in tomato production worldwide. We carried out Polymerase Chain Reaction studies to detect the presence of genes encoding antifungal compounds in the DNA of Pseudomonas putida strain PCI2. We also used liquid chromatography-electrospray tandem mass spectrometry to detect and quantify the production of compounds that increase the resistance of plants to diseases from culture supernatants of PCI2. In addition, we investigatedthe presence of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase in PCI2. Finally, PCI2 was used for inoculation of tomato seeds to study its potential biocontrol activity against Fusarium oxysporum MR193. The obtained results showed that no fragments for the encoding genes of hydrogen cyanide, pyoluteorin, 2,4-diacetylphloroglucinol, pyrrolnitrin, or phenazine-1-carboxylicacid were amplified from the DNA of PCI2. On the other hand, PCI2 produced salicylic acid and jasmonic acid in Luria?Bertani medium and grew in a culture medium containing ACC as the sole nitrogen source. We observed a reduction in disease incidence from 53.33 % in the pathogen control to 30 % in tomato plants pre-inoculated with PCI2 as well as increases in shoot and root dry weights in inoculated plants, as compared to the pathogenicity control. This study suggests that inoculation of tomato seeds with P. putida PCI2 increases the resistance of plants to root rot caused by F. oxysporum and that PCI2 produces compounds that may be involved at different levels in increasing such resistance. Thus, PCI2could represent a non-contaminating management strategy potentially applicable in vegetable crops such as tomato.","palabras_clave":"PSEUDOMONAS PUTIDA PCI2|BIOCONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":50,"idioma_id":4,"titulo":"Leukocyte counts in different populations of Antarctic Pygoscelid penguins along the Antarctic Peninsula","resumen":"The Antarctic Peninsula is one of the areas where the climate is changing at the fastest pace, having several effects on the populations of pygoscelid penguins. Few studies have analysed the variation in immune parameters of antarctic birds in a geographical context; thus, analyses of geographical differences in the immune components of wild pygoscelid penguins are still scarce. Leukocyte counts in birds provide information on their immunity and physiological stress. The objective of this study was to analyse the leukocyte counts in penguins of the genus Pygoscelis (gentoo, Adélie and chinstrap penguins), covering sites along the South Shetland Islands and some islands on the west coast of the Antarctic Peninsula. Our results revealed differences in the number of heterophils and eosinophils and in the heterophil\/lymphocyte ratio in the northeastern populations of gentoo and Adélie penguins as compared to the rest of the colonies studied. The results contribute to better understanding of the variations in physiological parameters of penguins related to a geographical context.","palabras_clave":"ANTARCTICA|GEOGRAPHICAL CONTEXT|LEUKOCYTE COUNTS|PYGOSCELID PENGUINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":53,"idioma_id":4,"titulo":"Aquatic microinvertebrate abundance and species diversity in peat bogs of Tierra del Fuego (Argentina)","resumen":"Peat bogs are regarded as extreme environments due to their low pH and low nutrient concentration, and thus hold a unique biota adapted to these particular conditions. The island of Tierra del Fuego encompasses the southernmost extensive peat bog area in the world, and is therefore particularly interesting from a biogeographical viewpoint. Within the same peat bog, different environment types can be identified: clear ponds, vegetated ponds and Sphagnum patches. In this study we compare the abundance, richness and species diversity of microinvertebrates (Copepoda, Cladocera and Rotifera) in these three types of environments from two peat bogs (Andorra and Rancho Hambre). Out of the 29 taxa recorded, 19 were common to both peat bogs, including four cladocerans endemic to Southern Patagonia and three rotifers endemic to Fuegian peat bogs. The rotifers were the dominant group in all environment types from Rancho Hambre, while in Andorra the Sphagnum moss was dominated by copepods, particularly harpacticoids. The results revealed that the environment type rather than peat bog was the key factor at explaining differences in species richness and diversity among microinvertebrate communities. This study highlights the importance of Sphagnum moss as a low diversity extreme environment which supports highly endemic species.","palabras_clave":"MICROINVERTEBRATES|PEAT BOGS|DIVERSITY|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":54,"idioma_id":4,"titulo":"The N-terminal pre-A region of Mycobacterium tuberculosis 2\/2HbN promotes NO-dioxygenase activity","resumen":"AbstractA unique defense mechanisms by which Mycobacterium tuberculosis protects itself from nitrosative stress is based on the O2-dependent NO-dioxygenase (NOD) activity of truncated hemoglobin 2\/2HbN (Mt2\/2HbN). The NOD activity largely depends on the efficiency of ligand migration to the heme cavity through a two-tunnel (long and short) system; recently, it was also correlated with the presence at the Mt2\/2HbN N-terminus of a short pre-A region, not conserved in most 2\/2HbNs, whose deletion results in a drastic reduction of NO scavenging. In the present study, we report the crystal structure of Mt2\/2HbN-&#916;preA, lacking the pre-A region, at a resolution of 1.53 Å. We show that removal of the pre-A region results in long range effects on the protein C-terminus, promoting the assembly of a stable dimer, both in the crystals and in solution. In the Mt2\/2HbN-&#916;preA dimer, access of heme ligands to the short tunnel is hindered. Molecular dynamics simulations show that the long tunnel branch is the only accessible pathway for O2-ligand migration to\/from the heme, and that the gating residue Phe(62)E15 partly restricts the diameter of the tunnel. Accordingly, kinetic measurements indicate that the kon value for peroxynitrite isomerization by Mt2\/2HbN-&#916;preA-Fe(III) is four-fold lower relative to the full-length protein, and that NO scavenging by Mt2\/2HbN-&#916;preA-Fe(II)-O2 is reduced by 35-fold. Therefore, we speculate that Mt2\/2HbN evolved to host the pre-A region as a mechanism for preventing dimerization, thus reinforcing the survival of the microorganism against the reactive nitrosative stress in macrophages. Database Coordinates and structure factors have been deposited in the Protein Data Bank under accession number 5AB8. Removal of the pre-A region in M. tuberculosis 2\/2HbN (Mt2\/2HbN-&#916;preA) results in dimerization and in a reduced access to the heme pocket. Kinetic measurements indicate a 4-fold decrease in kon for peroxynitrite isomerization and a 35-fold decrease in NO-scavenging relative to full-length Mt2\/2HbN. Thus, the pre-A region might be involved in reinforcing survival of the microorganism against nitrosative stress in macrophages.","palabras_clave":"M. Tuberculosis|truncated hemoglobin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":57,"idioma_id":5,"titulo":"Medicalización más allá de los médicos. Marketing farmacéutico en torno al Trastorno por Déficit de Atención e Hiperactividad en Argentina y Brasil (1998-2014)","resumen":"Desde un análisis crítico de los estudios de la medicalización, y como un aporte a estas perspectivas, describimos y analizamos los modos en que la industria farmacéutica transnacional penetra en diversos espacios sociales, con diferentes estrategias de marketing, interviniendo en la consolidación de procesos medicalizadores en Argentina y Brasil. Se analizan dos modalidades de expansión de los procesos de medicalización, y se desarrollan aspectos y tendencias específicas del diagnóstico y tratamiento del TDAH en ambos países: la incidencia de la industria farmacéutica en los grupos de apoyo en Brasil, y las estrategias de marketing farmacéutico orientadas a actores no médicos en Argentina. Estas dos modalidades se caracterizan por no involucrar sólo al profesional médico. La metodología incluye datos de investigaciones conducidas en Argentina y Brasil entre 1998 y 2014, con sede en la Universidad de Buenos Aires y la Universidade Estadual do Rio de Janeiro, orientadas al estudio de los procesos de diagnóstico y tratamiento del TDAH y el consumo de metilfenidato en ambos países. Se emplearon técnicas de entrevista semiestructurada individual y grupal a profesores y profesionales de salud, estadísticas oficiales y de organizaciones profesionales, y revisión de bibliografía general y especializada nacional e internacional. Concluimos que los fenómenos documentados en Argentina y Brasil ponen de relieve la importancia de efectuar investigaciones que contemplen aspectos singulares de los casos empíricos, y sus múltiples vinculaciones con entramados más amplios y en tensión de saberes, dispositivos, normativas y actores involucrados en la medicalización en el siglo XXI.","palabras_clave":"Medicalización|Grupo de apoyo|TDHA|Industria farmacéutica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":59,"idioma_id":4,"titulo":"Macro-Climatic Distribution Limits Show Both Niche Expansion and Niche Specialization among C4 Panicoids","resumen":"Grasses are ancestrally tropical understory species whose current dominance in warm open habitats is linked to the evolution of C4 photosynthesis. C4 grasses maintain high rates of photosynthesis in warm and water stressed environments, and the syndrome is considered to induce niche shifts into these habitats while adaptation to cold ones may be compromised. Global biogeographic analyses of C4 grasses have, however, concentrated on diversity patterns, while paying little attention to distributional limits. Using phylogenetic contrast analyses, we compared macro-climatic distribution limits among ~1300 grasses from the subfamily Panicoideae, which includes 4\/5 of the known photosynthetic transitions in grasses. We explored whether evolution of C4 photosynthesis correlates with niche expansions, niche changes, or stasis at subfamily level and within the two tribes Paniceae and Paspaleae. We compared the climatic extremes of growing season temperatures, aridity, and mean temperatures of the coldest months. We found support for all the known biogeographic distribution patterns of C4 species, these patterns were, however, formed both by niche expansion and niche changes. The only ubiquitous response to a change in the photosynthetic pathway within Panicoideae was a niche expansion of the C4 species into regions with higher growing season temperatures, but without a withdrawal from the inherited climate niche. Other patterns varied among the tribes, as macro-climatic niche evolution in the American tribe Paspaleae differed from the pattern supported in the globally distributed tribe Paniceae and at family level.","palabras_clave":"gramineas|fotosintesis|evolucion|expansion de nichos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":61,"idioma_id":4,"titulo":"Brucella abortus Omp19 recombinant protein subcutaneously co-delivered with an antigen enhances antigen-specific T helper 1 memory responses and induces protection against parasite challenge","resumen":"The discovery of effective adjuvants for many vaccines especially those with limited commercial appeal,such as vaccines to poverty-related diseases, is required. In this work, we demonstrated that subcutaneousco-administration of mice with the outer membrane protein U-Omp19 from Brucella spp. plus OVA as antigen (Ag) increases Ag-specific T cell proliferation and T helper (Th) 1 immune responses in vitro and in vivo. U-Omp19 treated dendritic cells promote IFN- production by specific CD4+ T cells and increases T cell proliferation. U-Omp19 co-administration induces the production of Ag specific effector memory T cell populations (CD4+ CD44high CD62Llow T cells). Finally, subcutaneous co-administration of U-Omp19 with Trypanosoma cruzi Ags confers protection against virulent parasite challenge, reducing parasitemiaand weight loss while increasing mice survival. These results indicate that the bacterial protein U-Omp19 when delivered subcutaneously could be a suitable component of vaccine formulations against infectious diseases requiring Th1 immune responses","palabras_clave":"Vacuna|adyuvante"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":62,"idioma_id":4,"titulo":"Brain Angiotensin II AT1 receptors are involved in the acute and long-term amphetamine-induced neurocognitive alterations","resumen":"Rationale: Angiotensin II, by activation of its brain AT1-receptors, plays an active role as neuromodulator in dopaminergic transmission. These receptors participate in the development of amphetamine-induced behavioral and dopamine release sensitization. Dopamine is involved in cognitive processes and provides connectivity between brain areas related to these processes. Amphetamine by its mimetic activity over dopamine neurotransmission elicits differential responses after acute administration or after re-exposure following long-term withdrawal periods in different cognitive processes. Objective: The purpose of this study is to evaluate the AT1-receptor involvement in the acute and long-term amphetamine-induced alterations in long-term memory and in cellular-related events. Methods: Male Wistar rats (250-300 g) were used in this study. Acute effects: Amphetamine (0.5\/2.5 mg\/kg i.p.) was administered after post-training in the inhibitory avoidance (IA) response. The AT1-receptor blocker Losartan was administered i.c.v. before a single dose of amphetamine (0.5 mg\/kg i.p.). Long-term effects: The AT1-receptors blocker Candesartan (3 mg\/kg p.o.) was administered for 5 days followed by 5 consecutive days of amphetamine (2.5 mg\/kg\/day, i.p.). The neuroadaptive changes were evidenced after 1 week of withdrawal by an amphetamine challenge (0.5 mg\/kg i.p.). The IA response, the neuronal activation pattern, and the hippocampal synaptic transmission were evaluated. Results: The impairing effect in the IA response of post-training acute amphetamine was partially prevented by Losartan. The long-term changes induced by repeated amphetamine (resistance to acute amphetamine interference in the IA response, neurochemical altered response, and increased hippocampal synaptic transmission) were prevented by AT1-receptors blockade. Conclusions: AT1-receptors are involved in the acute alterations and in the neuroadaptations induced by repeated amphetamine associated with neurocognitive processes.","palabras_clave":"ANGIOTENSIN II|AMPHETAMINE|HIPPOCAMPUS|LONG-TERM MEMORY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":63,"idioma_id":4,"titulo":"THE ROLE OF POLYSACCHARIDES ON THE STABILITY OF COLLOIDAL PARTICLES OF BEER","resumen":"This study aims at understanding the influence of beer colloidal composition and size on physical stability of the colloidal suspensions in beer before filtration. The percentages of retention during filtration indicate that colloidal particles consist principally of polysaccharides (97,04%) and in smaller amounts of proteins (2,87%) and polyphenols (0.22). The viscosity of a colloidal dispersion can be modeled as the sum of a hard-sphere contribution and a colloidal forces contribution. The colloidal force contribution is determined from the volume fraction of particles, the viscosity colloidal dispersion and the beer viscosity without particles. The volume fraction of particles could be estimated from the particle density and the total solid present in the beer. The fitting parameters of the correlation between the particles volume fraction and the colloidal force viscosity could explain the low stability of the colloidal particles of beer. This last determination is more specific than the simple viscosity of beer and could help to improve the prediction of the filtration flow.","palabras_clave":"HYDROCOLLOID|POLYSACCHARIDES|VISCOCITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":70,"idioma_id":4,"titulo":"Hybrid films based on a bridged silsesquioxane doped with goethite and montmorillonite nanoparticles as sorbents of wastewater contaminants","resumen":"The synthesis and characterization of silsesquioxane (SSO) films with pendant dodecyl groups and doped with goethite (Gt) or montmorillonite (MMT) nanoparticles were carried out and the new materials tested as sorbents of diverse contaminants. The synthetic method used yielded SSO films with the inorganic substrates homogeneously distributed within the polymeric matrices. The new materials were characterized by SEM, FTIR, XRD and DSC, and tested to evaluate their capability for adsorbing metallic cations, organic dyes and phosphate; frequent contaminants of industrial effluents. All films were found suitable for removing metallic cations. Results also showed that the SSO films undoped and doped with Gt are primarily apt for anionic compounds removal. Although the SSO films doped with MMT are capable to remove cationic contaminants from aqueous samples, the stiffness of the SSO matrix hinder MMT properties as an adsorbent. The possibility of dispersing nanoparticulate systems in the stable and chemically inert SSO matrices simplifies their application for contaminant removal, particularly because it makes easier the separation process of the absorbed pollutant from the treated medium.","palabras_clave":"SILSESQUIOXANE|GOETHITE|MONTMORILLONITE|WASTEWATER CONTAMINATS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":74,"idioma_id":4,"titulo":"POPULATION BALANCE DISCRETIZATION FOR GROWTH, ATTRITION, AGGREGATION, BREAKAGE AND NUCLEATION","resumen":"This paper presents a new discretization method to solve one-dimensional population balance equations (PBE) for batch and unsteady\/steady-state continuous perfectly-mixed systems. The numerical technique is valid for any size change mechanism (i.e., growth, aggregation, attrition, breakage and nucleation occurring alone or in combination) and different discretization grids. The developed strategy is based on the moving pivot technique of Kumar and Ramkrishna and the cellaverage method of Kumar et al. A novel contribution is proposed to numerically handle the growth and attrition terms, for which a new representation of the number density function within each size class isdeveloped. This method allows describing the number particle fluxes through the class interfaces accurately by preserving two sectional population moments.By comparing the numerical particle size distributions with analytical solutions of one-dimensional PBEs (including different size change mechanisms and particle-size dependent kinetics), the accuracy of the proposed numerical method was proved","palabras_clave":"POPULATION BALANCE EQUATION|PARTICLES SIZE DISTRIBUTION|DISCRETIZATION METHOD|SIZE CHANGE MECHANISMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":75,"idioma_id":4,"titulo":"SiO2-CaO-P2O5 (58S) sol gel glass applied onto surgical grade stainless steel by spray technique: morphological characterization by digital image processing","resumen":"AISI 316L stainless steel is commonly used as alow-cost material for permanent implants. It can be protectedfor degradation and corrosion by applying a hybridsilica based coating. Also the bioactive response of the implantcan only be achieved by functionalizing the coatedimplant surface. The aim of this work is to synthesize andcharacterize a sol-gel made glass particles from the systemSiO2-CaO-P2O5 with potential as bone inductive material,with and without an aging treatment of the precursorsolution. The glass was synthesized by sol-gel techniquethat, comparing with melt glasses, generates an open netstructure that could lead to particle dissolution and apatitedeposition for biological purposes. The synthesizedglass is dispersed by spray onto AISI 316L protected by ahybrid silica based coating, generating deposits with differentsize and morphology. To characterize the particlescomposition, microRaman spectroscopy was applied. Itshowed that no significant changes were reached after agingor thermal treatment of the deposited particles. Imageprocessing techniques based on Mathematical Morphologywere used to analyze morphology and sizes of the depositsobtain with the different sols (aged and no aged).Aproximately 50% of the surface was covered with particlesmade with a glass aged, and a 25% of covered areawas reached with no aged one. When no aged glass particleswere deposited, the particle size distribution showsthe presence of many big particles with a roundness factorbetween 0.8 and 1 in a high percentage, meaning thatthey are spherical due to the presence of solvent and witha more open glass structure in the no aged glass. The DigitalImage Processing and Raman spectroscopy tools helpto analyze, characterize and quantify the bioactive particlesdeposited onto coated surgical grade stainless steel interms of morphology, distribution and composition.","palabras_clave":"58S glass|Stainless steel coating|Spray de- position|Image analysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":77,"idioma_id":4,"titulo":"Determination of surface tension of surfactant solutions through capillary rise measurements. An image-processing undergraduate laboratory experiment","resumen":"In this work, we described an image processing procedure for the measurement of surface tension of the air&#8722;liquid interface using isothermal capillary action. The experiment, designed for an undergraduate course, is based on the analysis of a series of solutions with diverse surfactant concentrations at different ionic strengths. The objective of the work is to illustrate some concepts such as Gibbs isotherm and surface excess for the case of ionic surfactants. To perform the experiment, it is necessary to use glassware commonly found in a chemistry lab along with an USB microscope and digital image processing program (available in the publicdomain). The experiment can be completed in a 4 h laboratory session.","palabras_clave":"Physical Chemistry|Surface Science|Thermodynamics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":81,"idioma_id":4,"titulo":"Spatio-temporal genetic structure of the rodent Calomys venustus in linear, fragmented habitats","resumen":"Studies about habitat fragmentation, in terms of how it affects gene flow and genetic variability, have traditionally been conducted on island-like systems in which the remaining habitats form patches embedded in a matrix. However, in agroecosystems, remaining habitats usually form linear strips along fence lines, roads, and water courses (?border? habitats). We used the rodent Calomys venustus, a species inhabiting borders in central Argentina agroecosystems, as a model to address how genetic variability is structured in linear habitats. A total of 359 rodents were captured seasonally from spring 2005 to winter 2006. Genetic variability at microsatellite loci was uniformly high, despite significant variation in population size during the sampling period. Genetic differentiation, spatial autocorrelation, and causal modeling analyses suggested that dispersion patterns in this species depend mainly on geographic distance, with unfavorable habitat like dirt roads and crop fields posing only weak (or no) resistance to dispersal. Small-scale spatial genetic structure was related to different space use patterns by females and males. Our results showed that, although greatly reduced in area, border habitats can support stable populations of species without loss of either variability or genetic connectivity.","palabras_clave":"agroecosystem|Calomys venustus|dispersal|genetic structure|linear habitat|maintenance of genetic variability|microsatellite|spatial genetic autocorrelation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":85,"idioma_id":5,"titulo":"Espondilitis anquilosante en una población contemporánea de La Plata, Argentina","resumen":"Las espondiloartropatías son un grupo de patologías que afectan la espina dorsal caracterizadas por presentar morfología y factores genéticos predisponentes en común. Entre estas, la más frecuentemente hallada en restos óseos es la espondilitis anquilosante (EA), una enfermedad progresiva, de carácter inflamatorio, con etiología primaria aún desconocida. Siendo escasos los estudios de dicha patología en colecciones osteológicas, el objetivo de este trabajo es establecer su prevalencia en una colección documentada y contemporánea representativa de la Ciudad de La Plata, Argentina, a la vez que discutir los rasgos patognomónicos asociados. Los 100 esqueletos observados pertenecen a la Colección Osteológica Profesor Doctor Rómulo Lambre. Para el diagnóstico de la EA se formuló una definición operacional, consistente en la observación de fusión sacroilíaca, anquilosis vertebral en forma de ?Caña de Bambú? con compromiso zigoapofisiario y, en segundo orden, la artritis y\/o entesitis asimétrica en articulaciones periféricas. Condiciones relacionadas a la expresión de la EA fueron observadas en tres individuos, equivalentes al 3% de la muestra. Las regiones afectadas resultaron compatibles con aquellas detectadas a partir de estudios clínicos. Coincidentemente con estudios epidemiológicos modernos, se observó una mayor afección del sexo masculino a la vez que, el grado de desarrollo de la patología se relacionó con la edad de los individuos afectados. Fue comprobableademás cómo la posibilidad de contar con el esqueleto completo en buen estado de preservación facilitó el diagnóstico diferencial","palabras_clave":"ESPONDILITIS ANQUILOSANTE|CAÑA DE BAMBU|ESPONDILOARTROPATIAS|COLECCION LAMBRE|MUESTRA CONTEMPORANEA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":87,"idioma_id":4,"titulo":"The species pair Pseudocyphellaria pilosella-piloselloides (lichenized Ascomycota: Lobariaceae) is a single species","resumen":"The foliose lichens Pseudocyphellaria pilosella and P. piloselloides are characterized by a cyanobacterial photobiont, a tomentose upper surface, a yellow medulla and yellow pseudocyphellae. The latter species has since long been recognized as the sorediaate counterpart of the former. The morphological, anatomical, chemical, and molecular analyses performed for this study, support that they should be treated as single species.","palabras_clave":"HAPLOTPYES|LICHENS|SOUTHERN SOUTH AMERICA|SYNONYM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":90,"idioma_id":4,"titulo":"Opposite effects of dihydrotestosterone and estradiol on apoptosis in the anterior pituitary gland from male rats","resumen":"Hormones locally synthesized in the anterior pituitary gland are involved in regulation of pituitary cell renewal. In the pituitary, testosterone (T) may exert its actions per se or by conversion to dihydrotestosterone (DHT) or 17&#946;-estradiol (E2) by 5&#945;-reductase and aromatase activity, which are expressed in this gland. Previous reports from our laboratory showed that estrogens modulate apoptosis of lactotropes and somatotropes from female rats. Now, we examined the in vitro and in vivo effects of gonadal steroids on apoptosis of anterior pituitary cells from adult male rats. T in vitro did not modify apoptosis in anterior pituitary cells from gonadectomized (GNX) male rats. DHT, a non-aromatizable androgen, exerted direct antiapoptotic action on total anterior pituitary cells and folliculo-stellate cells, but not on lactotropes, somatotropes, or gonadotropes. On the contrary, E2 exerted a rapid apoptotic effect on total cells as well as on lactotropes and somatotropes. Incubation of anterior pituitary cells with T in presence of Finasteride, an inhibitor of 5&#945;-reductase, increased the percentage of TUNEL-positive cells. In vivo administration of DHT to GNX rats reduced apoptosis in the anterior pituitary whereas E2 exerted proapoptotic action and reduced cells in G2\/M-phase of the cell cycle. In summary, our results indicate that DHT and E2 have opposite effects on apoptosis in the anterior pituitary gland suggesting that local metabolization of T to these steroids could be involved in pituitary cell turnover in males. Changes in expression and\/or activity of 5&#945;-reductase and aromatase may play a role in the development of anterior pituitary tumors.","palabras_clave":"adenohipófisis|testosterona|estradiol|machos|apoptosis|aromatasa|5-alfa-reductasa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":92,"idioma_id":4,"titulo":"The trypanocidal benznidazole promotes adaptive response to oxidative injury: Involvement of the nuclear factor-erythroid 2-related factor-2 (Nrf2) and multidrug resistance associated protein 2 (MRP2).","resumen":"Oxidative stress is a frequent cause underlying drug-induced hepatotoxicity. Benznidazole (BZL) is the only trypanocidal agent available for treatment of Chagas disease in endemic areas. Its use is associated with side effects, including increases in biomarkers of hepatotoxicity. However, BZL potential to cause oxidative stress has been poorly investigated. Here, we evaluated the effect of a pharmacologically relevant BZL concentration (200&#956;M) at different time points on redox status and the counteracting mechanisms in the human hepatic cell line HepG2. BZL increased reactive oxygen species (ROS) after 1 and 3h of exposure, returning to normality at 24h. Additionally, BZL increased glutathione peroxidase activity at 12h and the oxidized glutathione\/total glutathione (GSSG\/GSSG+GSH) ratio that reached a peak at 24h. Thus, an enhanced detoxification of peroxide and GSSG formation could account for ROS normalization. GSSG\/GSSG+GSH returned to control values at 48h. Expression of the multidrug resistance-associated protein 2 (MRP2) and GSSG efflux via MRP2 were induced by BZL at 24 and 48h, explaining normalization of GSSG\/GSSG+GSH. BZL activated the nuclear erythroid 2-related factor 2 (Nrf2), already shown to modulate MRP2 expression in response to oxidative stress. Nrf2 participation was confirmed using Nrf2-knockout mice in which MRP2 mRNA expression was not affected by BZL. In summary, we demonstrated a ROS increase by BZL in HepG2 cells and a glutathione peroxidase- and MRP2 driven counteracting mechanism, being Nrf2 a key modulator of this response. Our results could explain hepatic alterations associated with BZL therapy.","palabras_clave":"MRP2|NRF2|OXIDATIVE STRESS|ADAPTIVE RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":93,"idioma_id":4,"titulo":"Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina","resumen":"More than 50 viruses have been reported incucurbit crops worldwide. In Argentina, cucurbit viruseshave been associated with important yield losses. Themost prevalent and widespread potyvirus is Watermelonmosaic virus (WMV). WMV was detected in Argentinain all cucurbit species with high incidence. In this study, aWMV isolate (WMV 1 SDE FF) was obtained from anaturally infected squash associated with a severe outbreakon melon and squash crops in an important cucurbitgrowing area in Santiago del Estero province (Argentina),during a survey conducted in November 2012. The fullysequenced WMV 1 SDE FF genome consists of 10,027nucleotides and shares 96 % nt identity and 98 % aaidentity with the French isolates JF273464.1|C07?014and EU660581.1|FMF00-LL1 of the WMV moleculargroup 3. Using the recombination detection programRDP4, two statistically significant recombination eventswere identified: event 1, an 830-nt long recombinantfragment in the putative P1 coding region, and event 2,a 4071-nt recombinant fragment detected across the HCPro, P3 and CI coding regions. The putative parentalsequences detected for event 1 were the EU660586.1|FBR04?37 (major parent) and JF273468.1|C07?284 (minorparent), both from France. Putative parental sequencesfor event 2 were JX079685.1| WMV-ShanXi(major parent) and HQ384216.1|Dendrobium (minor parent),from China and USA, respectively. To our knowledge,this is the first complete genome of an ArgentineWMVisolate. Our results provide evidence that WMV 1SDE FF is the causal agent of the strong outbreak reportedin melon and squash fields in recent years.","palabras_clave":"WMV|Cucurbitaceae|Virus recombination|Phylogenetic analysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":96,"idioma_id":4,"titulo":"Desiccating stress-induced disruption of ocular surface immune tolerance drives dry eye disease","resumen":"Dry eye is an allegedly autoimmune disorder for which the initiating mechanisms and the targeted antigens in the ocular surface are not known, yet there is extensive evidence that a localized Th1\/Th17 effector T cell response is responsible for its pathogenesis. In this work, we explore the reconciling hypothesis that desiccating stress, which is usually considered an exacerbating factor, could actually be sufficient to skew the ocular surface´s mucosal response to any antigen and therefore drive the disease. Using a mouse model of dry eye, we found that desiccating stress causes an NF-&#954;B- and time-dependent disruption of the ocular surface´s immune tolerance to exogenous ovalbumin. This pathogenic event is mediated by increased Th1 and Th17 T cells and reduced regulatory T cells in the draining lymph nodes. Conversely, topical NF-&#954;B inhibitors reduced corneal epithelial damage and IL-1&#946; and IL-6 levels in the ocular surface of mice under desiccating stress. The observed effect was mediated by an augmented regulatory T cell response, a finding that highlights the role of mucosal tolerance disruption in dry eye pathogenesis. Remarkably, the NF-&#954;B pathway is also involved in mucosal tolerance disruption in other ocular surface disorders. These results altogether suggest that targeting of mucosal NF-&#954;B activation could have therapeutic potential in dry eye. ","palabras_clave":"conjunctival tolerance|desiccating stress,|dry eye|mucosal tolerance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":98,"idioma_id":4,"titulo":"GIVING TAXONOMIC SIGNIFICANCE TO MORPHOLOGICAL VARIABILITY IN THE BIVALVE PTYCHOMYA AGASSIZ","resumen":"The taxonomic significance of the morphological variability present in the genus Ptychomya Agassiz has remained obscure due to inadequacy of the traditional qualitative approach to account for complex patterns of variation. In this work, we focus on solving the distinction betweenintra- and interspecific variability in Ptychomya from Hauterivian marine beds of the Neuquen Basin (west-central Argentina), a longstanding and unresolved palaeontological issue, using the quantitative framework provided by geometric morphometrics and multivariate statistics. The species Ptychomya koeneni Behrendsen and several subspecies had been recognized in the studied succession by former authors based on qualitative morphological descriptions. We tested two hypotheses: (1) that there exist morphological discontinuities between hypothesized species that mark their boundaries; and (2) that morphology follows a pattern of geographical variation, suitable for recognition of subspecies. We find two morphological groups in the studied sample, with a significant morphological gap between them, supporting the idea that there are at least two species in the studied succession, Ptychomya cf. koeneni and Ptychomya coihuicoensis Weaver. On the other hand, we found no correspondence between morphological and geographical distributions, leading to the conclusion that the named subspecies are invalid taxa. This study highlights the potential of this methodological approach for solving the taxonomy of this notorious genus of pandemic distribution during the Cretaceous.","palabras_clave":"Bivalvia|Morfometría Geométrica|Especie|Evolución"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":99,"idioma_id":4,"titulo":"Understanding the structure and function of Bacillus thuringiensis toxins.","resumen":"As biological control agents take an expanding share of the pesticides market and the production of insect-resistant crops increases, it is essential to understand the structure and function of the active agents, the invertebrate-active toxins that are the fundamental ingredients of these control systems. The potential for these agents in industry, agriculture and medicine necessitates a thorough investigation of their activity.","palabras_clave":"BACILLUS THURINGIENSIS|BT TOXINS|BT TOXIN STRUCTURE|MODE OF ACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":100,"idioma_id":4,"titulo":"Dehydroleucodine inhibits tumor growth in a preclinical melanoma model by inducing cell cycle arrest, senescence and apoptosis","resumen":"Malignant melanoma represents the fastest growing public health risk of all cancer types worldwide. Several strategies and anti-cancer drugs have been used in an effort to improve treatments, but the development of resistance to anti-neoplastic drugs remains the major cause of chemotherapy failure in melanomas. Previously, we showed that the sesquiterpene lactone, dehydroleucodine (DhL), promotes the accumulation of DNA damage markers, such as H2AX and 53BP1, in human tumor cells. Also DhL was shown to trigger either cell senescence or apoptosis in a concentration-dependent manner in HeLa and MCF7 cells. Here, we evaluated the effects of DhL on B16F0 mouse melanoma cells in vitro and in a pre-clinical melanoma model. DhL inhibited the proliferation of B16F0 cells by inducing senescence or apoptosis in a concentration-dependent manner. Also, DhL reduced the expression of the cell cycle proteins cyclin D1 and B1 and the inhibitor of apoptosis protein, survivin. In melanomas generated by subcutaneous injection of B16F0 cells into C57\/BL6 mice, the treatment with 20&#8201;mg DhL \/Kg\/day in preventive, simultaneous and therapeutic protocols reduced tumor volumes by 70%, 60% and 50%, respectively. DhL treatments reduced the number of proliferating, while increasing the number of senescent and apoptotic tumor cells. To estimate the long-term effects of DhL, a mathematical model was applied to fit experimental data. Extrapolation beyond experimental time points revealed that DhL administration following preventive and therapeutic protocols is predicted to be more effective than simultaneous treatments with DhL in restricting tumor growth.","palabras_clave":"SESQUITERPENE LACTONES|DEHYDROLEUCODINE|MELANOMA|MATHEMATICAL MODEL|APOPTOSIS|PREMATURE SENESCENCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":102,"idioma_id":4,"titulo":"Aerobic degradation of ibuprofen in batch and continuous reactors by an indigenous bacterial community","resumen":"Water from six points from the Riachuelo-Matanza basin was analyzed in order to assess ibuprofen biodegradability. In four of them biodegradation of ibuprofen was proved and degrading bacterial communities were isolated. Biodegradation in each point could not be correlated with sewage pollution. The indigenous bacterial community isolated from the point localized in the La Noria Bridge showed the highest degradative capacity and was selected to perform batch and continuous degradation assays. The partial 16S rRNA gene sequence showed that the community consisted of Comamonas aquatica and Bacillus sp. In batch assays the community was capable of degrading 100&#8197;mg&#8197;L&minus;1 of ibuprofen in 33&#8197;h, with a specific growth rate (&mu;) of 0.21&#8197;h&minus;1. The removal of the compound, as determined by High performance liquid chromatography (HPLC), exceeded 99% of the initial concentration, with a 92.3% removal of Chemical Oxygen Demand (COD). In a down-flow fixed-bed continuous reactor, the community shows a removal efficiency of 95.9% of ibuprofen and 92.3% of COD for an average inlet concentration of 110.4&#8197;mg. The reactor was kept in operation for 70 days. The maximal removal rate for the compound was 17.4&#8197;g&#8197;m&minus;3&#8197;d&minus;1. Scanning electron microscopy was employed to observe biofilm development in the reactor. The ability of the isolated indigenous community can be exploited to improve the treatment of wastewaters containing ibuprofen.","palabras_clave":"Emerging contaminants|ibuprofen|aerobic reactors|biodegradation|matanza riachuelo basin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":108,"idioma_id":4,"titulo":"F8 intron 22 inversions and SNP rs73563631 in unrelated families with severe haemophilia A: clinical features and gene testing implications","resumen":"Herein, we performed an INV22 rescreening of our historical series of 308 families with severe-HA using ISPCR\/2008-DT\/-CT. This allowed characterisation of 143 families with conventional INV22 (46 %): 120 with INV22?1 (84 % of INV22s) and 23 INV22?2 (16 %). Two families (0.65 %) showed unusual ISPCR\/2008 patterns characterised by no signals in the IS-PCR\/2008-DT and conventional INV22?1\/-2 patterns in the IS-PCR\/2008-CT.","palabras_clave":"F8|haemophilia|inversion|SNP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":115,"idioma_id":4,"titulo":"On the evolution and diversification of an Andean clade of reptiles: combining morphology and DNA sequences of the palluma group (Liolaemidae: Phymaturus)","resumen":"Phymaturus comprises 44 species mainly distributed along the south-west of South America on both sides of the Andes. In this study we present a phylogenetic analysis of Phymaturus of the palluma group, one of its two large clades, including almost all described species. This analysis duplicates the number of  n-group taxa compared with previous contributions. We performed a total-evidence analysis, combining molecular and morphological characters: sequencing genes of cytochome b (cytb), 12S, and ND4, for all terminals; describing 45 new morphological characters; and incorporating all DNA sequences available from GenBank. Separate analyses of morphology and DNA partitions are presented and discussed in detail. Seven subclades are recognized here. We named three newsubclades and redefined another, found to be paraphyletic. In order to recognize lineages within the traditional Phymaturus palluma group we proposed to treat it as a natural group, containing within it the ranks of clade, subclade, and lineages, respectively. The palluma group is composed by the vociferator and the bibronii clades. The vociferator clade, composed of Chilean and Argentinean species, would be the most basal in the group. Within the bibronii clade, the roigorum subclade includes the Phymaturus verdugo lineage, whereas the mallimaccii subclade would consist of 13 terminal taxa, for which three Chilean species have been added. In this study, morphological apomorphies are identified for all clades and the evolution of ?male head melanism? is discussed.","palabras_clave":"Phylogenetic relationships|Phymaturus|Taxonomic congruence|total evidence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":118,"idioma_id":4,"titulo":"FISH-mapping of the 5S rDNA locus in the chili peppers (Capsicum-Solanaceae)","resumen":"We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position was found. 5S FISH signals of nearly similar size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid to the cytomolecular characterization of pepper germplasm to evaluate variability and will be instrumental to integrate physical, genetic and genomic maps already generated in the genus.","palabras_clave":"WILD AND CULTIVATED CAPSICUM|5S RRNA GENE|CAPSICUM-DERIVED PROBE|FISH-MAPPING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":120,"idioma_id":4,"titulo":"3D Formation Control of Autonomous Vehicles Based on Null-Space","resumen":"This paper proposes a new algorithm for controlling a formation of multiple autonomous aerial vehicles based on multiple control objectives. The strategy includes using the null space of a Jacobian matrix to achieve the different control objectives in a non-conflicting way. The mission is split into two elementary tasks, with suitable command references generated for each robot. The commands for each task are combined through a hierarchical method by using the projection of commands onto the null space. The incorporation of ground vehicles in the control scheme is also considered, thus extending the proposed scheme for controlling heterogeneous formations. The stability analysis of the control system shows that such a system is asymptotically stable, and experimental results validate the proposed control system. ","palabras_clave":"Aerial formation control|Null space|Quadrotor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":126,"idioma_id":5,"titulo":"CRYPHAEA CONSIMILIS (CRYPHAEACEAE, BRYOPHYTA), NUEVA CITA PARA LA ARGENTINA","resumen":"Cryphaea consimilis Mont. (Cryphaeaceae, Bryophyta) se registra por primera vez para la Argentina, extendiendo su distribución dentro de los bosques subantárticos. Se presenta una descripción breve, discusión, mapa de distribución e ilustración de la especie. En este trabajo se designa un lectotipo para la especie.","palabras_clave":"BRYOPHYTA|CRYPHAEACEAE|MUSGOS|NUEVO REGISTRO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":129,"idioma_id":4,"titulo":"Effects of waterborne exposure to 17&#946;-estradiol and 4-tert-octylphenol on early life stages of the South American cichlid fish Cichlasoma dimerus","resumen":"Estrogenicchemicalsareoftendetectedintheaquaticenvironmentandcannegativelyaffectanimaldevelopment andreproduction.Inteleost fishes, thehormonalregulationduringacriticalperiodoflarvaldevelopmenthasastronginfluence ongonadalsexdifferentiation;thusthisprocessmaybeaf-fected bytheexposuretoenvironmentalestrogens.Inthisstudy,we first assessedthelethalacutetoxicityofthenaturalestrogen17&#946;-estradiol(E2) andtheweakerestrogenmimics4-tert-octylphenol(OP) and4-nonylphenol(NP)onlarvalstagesoftheSouthAmericancichlid fish Cichlasomadimerus. Inafurther experiment,weanalyzedtheeffectsofchronicwaterborneexposuretoE2 and OPongonaddevelopment andsexdifferentiation.ExposuretohighconcentrationsofE2 had apronouncedfeminizingeffect directingsexdifferentiationtowardsovariandevelopment,whiletestisdevelopmentwasinhibitedat alower,environmentallyrelevantconcentration.AmongOP-exposed fish, 15?38.5% ofthemalesex-hibited testicularoocytes(TOs),acommonlyreportedbiomarkerofestrogenicexposure.However,sinceTOs werealsorecordedincontrolmalesandtheproportionofmaleswithTOswasnotsignificantlyhigher inOPtreatments,theiroccurrencecouldnotbeattributedtoOPexposure.Inaddition,TOsdidnotseem toimpairmalegonaddevelopmentandfunctionalitysincenormalspermatogenesiswasobservedin testesofOP-treated fish. TheseresultsindicatethatE2 occurring intheSouthAmericanaquaticen-vironment mayaffectmalereproductivedevelopmentandposeariskforwild C. dimerus, especiallyunder prolongedexposure,whiletheeffectsofweakerxenoestrogenssuchasOPwouldbenegligibleforgonad developmentinthisspecies.Asillustratedbythisstudy,thenaturaloccurrenceofTOsindicatesthat conclusionsconcerningthecausesofthisphenomenonmustbedrawnwithcare.","palabras_clave":"17B estardiol|octylphenol|early life stages|cichlid fish"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":131,"idioma_id":5,"titulo":"Mujeres migrantes, trabajo y empoderamiento. Bolivianas en una ciudad de la periferia globalizada","resumen":"RESUMEN: Ante la fuerte feminización de las migraciones internacionales en las grandes urbes del Norte y el Sur, interesa cuestionar las relaciones de género, trabajo y migración en ciudades medias en contextos de globalización, desde la geografía del género. Comodoro Rivadavia, ciudad de la Patagonia Argentina, ligada a la economía global, tanto del petróleo como de la pesca, está creciendo aceleradamente y atrae nuevos flujos migratorios, sobre todo, de bolivianos y bolivianas. Es una migración familiar marcada por una división sexual del trabajo, con evidencias de empoderamiento de las mujeres. Este artículo analiza la capacidad de agencia en cuanto a la inserción ocupacional de las bolivianas, que muestra las diferencias y el pasaje desde posiciones de subordinación a las de empoderamiento, a partir de sus trayectorias migratorias, analizadas mediante técnicas cualitativas. El artículo distingue, primero, dentro de la diversidad migratoria de Comodoro Rivadavia, la presencia de mujeres migrantes con foco en las bolivianas. Segundo, examina los tres tipos de ocupaciones de estas mujeres, unas asalariadas y precarizadas como obreras en industrias pesqueras y en el sector doméstico más el tipo propio de las comerciantes emprendedoras. Por último, cuestiona el impacto de esas relaciones de género y de trabajo a través de la apropiación del espacio y de la movilidad cotidiana dentro del espacio urbano. La publicación esta prevista para 2016 pero se incluye en este periodo pues el trabajo se desarrollo entre 2014 y 2015. Palabras clave: mujeres migrantes bolivianas; trabajo; empoderamiento; movilidad; Comodoro Rivadavia[Indexada Latindex, Redial, HAPI, DOAJ, OAlster, Scirus, Google Scholar, Ojose]","palabras_clave":"AGENCIA|EMPODERAMIENTO|FAMILIA|REDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":139,"idioma_id":4,"titulo":"Impact of X-rays on cytological traits of Tuta absoluta (Lepidoptera: Gelechiidae)","resumen":"The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most important pests of tomato. With the purpose of developing environmentally friendly control tactics such as the inherited sterility (IS) technique against this species, it is essential to understand its genetics and biology. In this paper we analyzed the karyotype, sperm morphology and sperm ratio in wild-type and X-ray irradiated individuals of T. absoluta. The diploid chromosome number of T. absoluta was 2n = 58 including the pair of sex chromosomes: ZZ in males and WZ in females, which were the largest elements of the complement. Irradiation of pupae in an X-ray machine with a dose of 200 Gy generated various types of chromosomal rearrangements including translocations and fragmentations, resulting in altered chromosome numbers. The analysis of spermatozoa in T. absoluta revealed a significant morphological difference between apyrene and eupyrene sperm bundles. Irradiation with X-ray doses of 100, 150, 200 and 250 Gy did not have a significant effect on the apyrene to eupyrene sperm ratio. However, males irradiated with 300 Gy produced significantly more apyrene sperm than non-irradiated males. All the doses applied influenced the morphology of eupyrene sperm bundles. The modified eupyrene sperm bundles could be used as a bioindicator during the monitoring of an IS program after the release of irradiated males. We found that the modified eupyrene spermatozoa were transferred to the bursae copulatrices of the females. Males treated with 200 Gy transferred a greater proportion of modified eupyrene sperm than untreated males. The results presented herein provide essential information on the cytology of T. absoluta, which is required to evaluate the quality of the released insects, and for better understanding and application of IS against this economically important pest.","palabras_clave":"Esterilidad heredada|Citología|Espermatogénesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":147,"idioma_id":5,"titulo":"Con buen diseño, espaldas sanas Recomendaciones para el diseño y la producción de mobiliario escolar saludable","resumen":"El mobiliario escolar puede constituir una concausa para la conformación saludable de la estructura ósea de niños y adolescentes. Este trabajo presenta un análisis de la postura sedente durante las actividades escolares; procura encontrar una relación entre el diseño del mobiliario utilizado actualmente en Mendoza y los factores posturales y rendimiento escolar de los alumnos. Dicho estudio fue subsidiado por la Secretaría de Ciencia, Técnica y Posgrado de la UNCUYO (SECTYP) en el período 2009-2011 y entre 2011-1013. Por su naturaleza, esta investigación es de característica ?tecnológica? aplicándose para su desarrollo el método propio de la Ergonomía basado en el ?enfoque sistémico?. Comprende las siguientes etapas: análisis y diagnóstico del mobiliario en uso, análisis de tareas, diseño de la propuesta, experimentación y validación. Se desarrollaron modelos de prueba bajo cánones ergonómicos de postura saludable para el aprendizaje, compuestos por silla y pupitre independientes. El resultado del estudio comprende una guía de recomendaciones para el diseño y la producción de mobiliario escolar saludable. El producto fue transferido a la Dirección General de Escuelas y el Ministerio de Infraestructura del Gobierno de Mendoza, y al Instituto Nacional de Tecnología Industrial (INTI), para su Programa de Diseño.","palabras_clave":"sedestación|mobiliario escolar|ergonomía|postura saludable"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":152,"idioma_id":4,"titulo":"Distribution patterns of the abundance of major bacterial and archaeal groups in Patagonian lakes","resumen":"We explored the distribution patterns of bacterial and archaeal abundances at the phylum and class level using cata- lyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH), examining the among (across 35 water bodies) and within-lake (intra-annual seasonality) patterns in Patagonia (Argentina). Alphaproteobacteria, Betaproteobacteria and Actinobacteria globally dominated the bacterioplankton, whereas Gammaproteobacteria and Archaea never exceeded 3 and 6% of the community, respectively. The different groups showed seasonality, with simultaneous peaks of all bacter- ial group absolute abundances during late winter or spring, and with peaks of Archaea during winter, late spring and summer. The bacterial groups presented roughly similar relative abundances in all seasons, whereas Archaea varied in their relative contribution to community structure. Multivariate analyses showed that dissolved organic carbon was an important variable structuring the community at the studied taxonomic resolution (using absolute and relative abun- dances), in both among and within-lake patterns. The absolute abundance of most bacterial groups was significantly higher in mesotrophic and eutrophic systems than in oligotrophic ones (except Actinobacteria), whereas their relative abundances did not change among trophic states (except Bacteroidetes). The lake grouping obtained from CARD-FISH was consistent with previous work using polymerase chain reaction-denaturing gradient gel electrophoresis data: deep oligotrophic lakes clustered together, whereas small and shallow water bodies grouped separately.","palabras_clave":"Bacteria|Archaea|patagonian water bodies|prokaryotic group distribution patterns|CARD-FISH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":154,"idioma_id":4,"titulo":"Cell therapy for Parkinson's disease: Functional role of the host immune response on survival and differentiation of dopaminergic neuroblasts","resumen":"Parkinson ´s Disease (PD) is a neurodegenerative disorder, whose cardinal pathology is the loss of dopaminergic neurons in the substantia nigra. Current treatments for PD have side effects in the long term, do not halt disease progression or regenerate dopaminergic cell loss. Attempts to compensate neuronal cell loss by transplantation of dopamine-producing cells started more than 30 years ago, leading to several clinical trials. These trials showed safety and variable efficacy among patients. In addition to variability in efficacy, several patients developed graft-induced dyskinesia. Nevertheless, they have provided a proof of concept that motor symptoms could be improved by cell transplantation.Cell transplantation in the brain presents several immunological challenges. The adaptive immune response should be abolished to avoid graft rejection by the host. In addition, the innate immune response will always be present after transplanting cells into the brain. Remarkably, the innate immune response can have dramatic effects on the survival, differentiation and proliferation of the transplanted cells, but has been hardly investigated.In this review, we analyze data on the functional effects of signals from the innate immune system on dopaminergic differentiation, survival and proliferation. Then, we discussed efforts on cell transplantation in animal models and PD patients, highlighting the immune response and the immunomodulatory treatment strategies performed. The analysis of the available data lead us to conclude that the modulation of the innate immune response after transplantation can increase the success of future clinical trials in PD by enhancing cell differentiation and survival","palabras_clave":"CELL THERAPY|DOPAMINERGIC NEURON|IMMUNE RESPONSE|PARKINSON DISEASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":158,"idioma_id":4,"titulo":"Epoxy Formulation Including an Acrylic Triblock Copolymer Adapted for Use in Filament Winding or Infusion Processes","resumen":"Commercial triblock copolymers with a poly(butyl acrylate) (PBuA) central block joined to two poly(methyl methacrylate) (PMMA) end blocks (denoted as MAM) or to two random copolymers end blocks based on MMA and N,N&#8242;-dimethylacrylamide (DMA) (denoted as MAM-N), can be employed as toughening agents for thermoset composites. However, their use in epoxy formulations for filament winding, requiring low viscosities during the fiber-impregnation step associated with an adequate glass transition temperature of the cured product is not trivial. In this study, we show that a blend of diglycidylether of bisphenol A (DGEBA), 4,4&#8242;-diamino-3,3&#8242;-dimethyldicyclohexylmethane (3DCM) and benzylamine (BA), with 20% of amine hydrogens provided by BA, and containing 5 wt% MAM, can be used for these purposes. The addition of MAM increased the critical stress intensity factor from 0.63 MPa.m1\/2 to 1.0 MPa.m1\/2, the glass transition temperature from 138°C to 145°C, and the glassy modulus at 25°C from 2.95 GPa to 3.15 GPa. MAM was a better choice for the envisaged applications than MAM-N because it led to solutions of lower viscosity. The higher viscosity produced by MAM-N was explained by specific interactions between the epoxy-amine solvent and DMA units present in its terminal blocks. POLYM. ENG. SCI., 56:1153?1159, 2016. © 2016 Society of Plastics Engineers.","palabras_clave":"Acrylic triblock copolymers|Nanostrength block copolymers|Reactive diluent|Toughened epoxies| Toughening agent"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":159,"idioma_id":5,"titulo":"DIAGNOSTICO AMBIENTAL EN LA ZONA DEL CINTURÓN HORTÍCOLA DE LA CIUDAD DE SANTA FE","resumen":"El cinturón hortícola de la ciudad de Santa Fe ubicado en el norte del ejido urbano. En dicha zona la provisión de agua se realiza con perforaciones domiciliarias que conviven con pozos negros y actividades hortícolas. Las actividades que se desarrollan representan importantes amenazas de contaminación, y además el peligro de contaminación podría incrementarse por un aumento de la vulnerabilidad dada por el ascenso de los niveles de agua subterránea y\/o aumento de la capacidad de transporte de contaminantes al ambiente subterráneo provocado por inundaciones fluviales o pluviales.La elaboración del diagnóstico ambiental que aquí se presenta fue construido sobre la base de estudios antecedentes, entrevistas, encuestas y relevamientos de campo, muestreos y análisis de laboratorio. Se caracterizaron prácticas agrícolas y actividades que se desarrollan en la zona asignándoles una categoría según la amenaza de contaminación del agua subterránea. Se relevaron especies cultivadas, agroquímicos, riego, aspectos socioculturales, forma de vida de los habitantes y características relacionadas al saneamiento, utilización del recurso y compromiso con el ambiente por parte de la población. Sobre la base de los resultados obtenidos se plantean algunos lineamientos de gestión y protección del recurso hídrico subterráneo, orientados al desarrollo ambientalmente sostenible de la zona","palabras_clave":"AGUAS SUBTERRANEAS|AGROQUIMICOS|HORTICULTURA|CONTAMINACION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":162,"idioma_id":4,"titulo":"Increase of calcium and reduction of lactose concentration in milk by treatment with kefir grains and eggshell","resumen":"Dairyproducts are the main source of calcium, but the loss of the consumption habitcontributes to low consumption in adulthood, which contributes to osteoporosisand increased fracture risk. Domestic use of kefir is straightforward and theeggshell is a natural discarded source of calcium. This paper proposes thedevelopment of an enriched calcium reduced lactose milk using eggshell andkefir. During the in vitro preparation, the pH, calcium and lactosecontent were measured. Intestinal absorption of untreated milk and milk withkefir was compared. Finally, human volunteers consumed this dairy product and24h-urine calcium was measured. Results showed that the beverage has lowerlactose and higher calcium than untreated milk. Intestinal calcium absorptionwas not different between both milks and an increase in urinary calciumexcretion was observed in humans. This study provides a methodology to prepareat home a dairy product that could contribute to improve the calcium intake inadults.","palabras_clave":"CALCIUM|DAIRY MILK|EGGSHELL|KEFIR GRAINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":169,"idioma_id":4,"titulo":"OBJECTIVE MEASUREMENT OF Akodon budini DORSAL COLORATION: METHODOLOGICAL CONCERNS","resumen":"The role of color in taxonomical and systematical studies of several taxa is central. Color data are more valuable if they permit comparisons. We aimed to provide pelage objective color measurements of a series of Akodon budini and to use those color data to test and to quantitatively analyze the influence of both the lighting source as the measuring point, in the determination of pelage color of museum skins. We used a spectroradiometer to measure the pelage color at five points over the dorsal midline of 54 Akodon budini museum skins. PCAs and ANOVAs were conducted over the color data. The characteristics of the studied series also allowed us to include and assess the potential effects of some main sources of intra-specific variation. Determinations of fur color strongly depend on the lighting source, and therefore it is essential that lighting conditions are controlled during color measurements and then made explicit when communicating the color characterization of particular taxonomic units. Also, color determinations strongly depend on the measuring point, even in the same body area, and therefore it is critical that color characterization of a taxon includes the variation between different points of the different body parts of the studied specimens.","palabras_clave":"CHROMATIC COORDINATES|CIELab COLOR SPACE|LIGHTING SOURCE|MEASURING POINTS|SPECTRORADIOMETER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":173,"idioma_id":5,"titulo":"Más allá de las pantallas: experiencias en diseño y programación de objetos interactivos digitales","resumen":"El software es un elemento cada vez más presente en la vida cotidiana de las personas, tanto a nivel profesional como personal. El desarrollo de saberes y habilidades relacionadas con la resolución de problemas y su programación puede ayudar a dar una mayor libertad de acción y de pensamiento a la hora de enfrentar la cotidianeidad. Desde la Universidad Pedagógica de la Provincia de Buenos Aires realizamos tareas de investigación orientadas a indagar cómo se puede desarrollar la fluidez digital en jóvenes y docentes. &nbsp;En este artículo se relata una experiencia relacionada con talleres, donde se trabajaron aspectos vinculados con el diseño, desarrollo y programación de objetos interactivos digitales. En el proyecto se utilizó la plataforma abierta Arduino. A partir del trabajo realizado se observó que el recurso es pertinente y encierra riqueza, para desarrollar una propuesta educativa de introducción al pensamiento computacional.","palabras_clave":"objetos interactivos|fluidez digital|arduino"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":185,"idioma_id":4,"titulo":"A polyhedral study of the maximum stable set problem with weights on vertex-subsets","resumen":"Abstract Given a graph G = ( V , E ) , a family of nonempty vertex-subsets S &#8838; 2 V , and a weight w : S &#8594; R + , the maximum stable set problem with weights on vertex-subsets consists in finding a stable set I of G maximizing the sum of the weights of the sets in S that intersect I . This problem arises within a natural column generation approach for the vertex coloring problem. In this work we perform an initial polyhedral study of this problem, by introducing a natural integer programming formulation and studying the associated polytope. We address general facts on this polytope including some lifting results, we provide connections with the stable set polytope, and we present three families of facet-inducing inequalities.","palabras_clave":"MAXIMUM STABLE SET|INTEGER PROGRAMMING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":187,"idioma_id":4,"titulo":"Lithic materials in high fluvial terraces of the central Pyrenean piedmont (Ebro Basin, Spain)","resumen":"Lithic materials from Lower Palaeolithic are scarce in the Ebro Basin. Archaeological surveys carried out in some alluvial terraces on central Pyrenean piedmont have unearthed three new sites with remains of lithic industries related to cobble and core technology and large cutting tools. In the upper terrace levels (T1 and T2) from the Alcanadre and Cinca rivers, some pieces have been recovered on the surfaces: cobble-tools at Las Fitas and bifacial tools at San Quílez. The approximate age of these levels ranges between 780 ka and 1000 ka, established by paleomagnetism and evolutionary stages of calcrete. A cleaver was interbedded into a terrace (T5 level) of a minor tributary of the Cinca River (Olriols), whose age has been established at a regional scale between 178\/151 ka. This T5 level is related to one of the cold phases of greater advance of Pyrenean glaciers during MIS 6.These new findings help us to widen our knowledge of a poorly known period of the human occupation of the middle Iberian basin. The chronological imprecision of most of the lithic materials has been partially overcome by means of geomorphological data, which offers a generic chrono-cultural framework for the people who used them.","palabras_clave":"PLEISTOCENE|LOWER PALEOLITHIC|MODE 1 AND 2|FLUVIAL TERRACES|CALCRETE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":193,"idioma_id":4,"titulo":"Solenoporacean red algae from lower Danian (Paleogene) coral reefs of northern Patagonia, La Pampa, Argentina","resumen":"THE Records of hermatypic coral reefs after the Cretaceous-Paleogene boundary are very scarce and their evolution during this seminal time interval is poorly understood. The finding in northern Patagonia, Argentina of Danian coral reefs is particularly significant. In this paper we describe a Solenoporacean red algae that is abundant as a component in a coral reef community included in the Roca Formation at Lomita Baya, La Pampa, Argentina","palabras_clave":"Solenoporacean|Algae|Danian|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":194,"idioma_id":5,"titulo":"Medición y Manipulación de Normas Sociales en Juegos Experimentales de Corrupción","resumen":"Los  estudios experimentales de coimas investigan los factores que modulan los  comportamientos vinculados a estas situaciones de corrupción. Para que capten  conductas análogas a la realidad es necesario que representen de manera  apropiada las normas sociales que se ven transgredidas cuando hay corrupción.  En esta revisión presentamos críticamente tres técnicas para medir o generar  normas sociales en experimentos económicos: el efecto de marco, la estimación  de normas mediante un juego de coordinación, y el castigo de terceros. Discutimos  cómo la complementariedad y el mejoramiento de estas técnicas pueden contribuir  a la validez externa de los estudios experimentales de corrupción.","palabras_clave":"experimentos|toma de decisiones|normas|castigo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":198,"idioma_id":5,"titulo":"La ?gripe española? en perspectiva médica: los brotes de 1918- 1919 en la escena científica argentina","resumen":"Objetivo:en el presente estudio nos proponemos examinar las ideas y conceptos quecirculaban en la ciencia médica argentina acerca de ciertos rasgos propios dela ?gripe española? que azotó a la población mundial entre 1918 y 1919 (origengeográfico, etiología, clínica, transmisibilidad, etc). La pandemia ingresó ala Argentina suscitando temor en la sociedad, confusión en las elites médicas yel impulso de medidas públicasoriginadas a los fines de luchar contra la enfermedad. Desarrollo: anclados en un abordaje decarácter cualitativo, pondremos en perspectiva analítica un conjunto deconferencias científicas llevadas a cabo en el país durante el desarrollo de la crisis epidémica como asítambién artículos médicos colocados en editoriales específicas vinculados a latemática en cuestión luego de culminada la pandemia. Conclusiones: la ?gripe española? se trató de una enfermedad generadora de múltiplesincógnitas. Consideramos que la carencia de certezas médicas determinó eladvenimiento de una serie de medidas públicas infructuosas, escasamentearticuladas entre sí, que no lograron mitigar la dolencia. Como contrapartida,lo que se obtuvieron fueron respuestas negativas, de descontento, por parte deuna población que se hallaba atemorizada ante un flagelo que no cesaba y queirrumpía en sus prácticas cotidianas. ","palabras_clave":"EPIDEMIA|GRIPE|MÉDICOS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":200,"idioma_id":5,"titulo":"Interacción entre conservación e investigación en el Área de Antropología Biológica del Museo Etnográfico J. B. Ambrosetti (Facultad de Filosofía y Letras, Universidad de Buenos Aires)","resumen":"Se detalla parte de las actividades realizadas en el marco de un proyecto de conservación en el Área de Antropología Biológica del Museo Etnográfico J. B. Ambrosetti, Universidad de Buenos Aires, Argentina. Este Plan Integral de Manejo de Colecciones Osteológicas se propone desarrollar un protocolo basado en el tratamiento ético de los restos humanos, destacándose los conceptos de custodia, identidad y respeto, que cubre aspectos articulados en cuatro ejes principales: ética, educación\/investigación, conservación y patrimonio. Dentro de ese marco, se discuten los procedimientos específicos implementados tendientes a la salvaguarda y minimización del deterioro de un conjunto de restos humanos afectados por agentes medioambientales. Se enfatiza en la interacción entre especialistas de diferentes disciplinas para desarrollar tareas de conservación desde una perspectiva integradora. Esta forma de abordar las colecciones establece nuevas estrategias que contemplan la necesidad de desarrollar tareas de conservación y analizar la información científica en un marco que priorice los aspectos bioéticos de las políticas de manejo patrimonial. Los resultados son prometedores para la caracterización de las colecciones, lo cual incluye la identificación, documentación, conservación y guarda de restos humanos prehistóricos procedentes de diferentes regiones del mundo. La aplicación de esta propuesta surge desde la bioantropología para abordar las investigaciones de restos humanos desde una perspectiva integral. Se pretende contribuir a generar conciencia entre los investigadores de restos humanos sobre la importancia de su participación en la generación de protocolos de conservación.","palabras_clave":"PLAN DE MANEJO|RESTOS HUMANOS|INVESTIGACION|INTERDISCIPLINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":202,"idioma_id":5,"titulo":"Estudios experimentales de la percepción desde la profesionalización de la  psicología en Argentina","resumen":"Se indaga el lugar que ocuparon los estudios experimentales sobre lapercepción en la psicología argentina tras la profesionalización de lapsicología. Se describen los tres grupos que sistemáticamente se dedicarona dicho tema desde la década de 1960 a la década de 1980: CIAL, LIS yUNCy\/UNSL. Se examinan sus figuras principales y sus líneas deinvestigación. Se indaga el grado de cohesión que alcanzaron comocomunidad científica nacional. Desde una perspectiva policéntrica, sedescribe su inserción internacional. Se discuten aspectos institucionales yculturales propios de las carreras de psicología argentinas que incidieron enla limitación del desarrollo de los estudios experimentales sobre lapercepción.","palabras_clave":"psicologia experimental|percepcion|laboratorios|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":209,"idioma_id":4,"titulo":"Novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases","resumen":"This work aimed to characterize potentially endophytic culturable bacteria from leaves of cultivated tomato and analyze their potential for growth promotion and biocontrol of diseases caused by Botrytis cinerea and Pseudomonas syringae. Bacteria were obtained from inner tissues of surface-disinfected tomato leaves of field-grown plants. Analysis of 16S rRNA gene sequences identified bacterial isolates related to Exiguobacterium aurantiacum (isolates BT3 and MT8), Exiguobacterium spp.(isolate GT4), Staphylococcus xylosus (isolate BT5), Pantoea eucalypti (isolate NT6), Bacillus methylotrophicus (isolate MT3), Pseudomonas veronii (isolates BT4 and NT2), Pseudomonas rhodesiae (isolate BT2) and Pseudomonas cichorii (isolate NT3). After seed inoculation, BT2, BT4, MT3, MT8, NT2 and NT6 were re-isolated from leaf extracts. NT2, BT2, MT3 and NT6 inhibited growth of Botrytis cinerea and Pseudomonas syringae pv.tomato in vitro, produced antimicrobial compounds and reduced leaf damage caused by B. cinerea. Some of these isolates also promoted growth of tomato plants, produced siderophores, the auxin indole-3-acetic and solubilized inorganic phosphate. Thus, bacterial communities of leaves from field-grown tomato plants were found to harbor potentially endophytic culturable beneficial bacteria capable of antagonizing pathogenic microorganisms and promoting plant growth, which could be used as biological control agents and biofertilizers\/biostimulators for promotion of tomato plant growth.","palabras_clave":"ENDOPHYTIC CULTURABLE BACTERIA|BOTRYTIS CINEREA|PSEUDOMONAS SYRINGAE|BIOCONTROL|GROWTH PROMOTION|ANTIMICROBIAL COMPOUNDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":210,"idioma_id":4,"titulo":"Progress on the description of identifying code polyhedra for some families of split graphs","resumen":"The identifying code problem is a newly emerging search problem, challenging both from a theoretical and a computational point of view, even for special graphs like bipartite graphs and split graphs.   Hence, a typical line of attack for this problem is to determine minimum identifying codes of special graphs or to provide bounds for their size.     In this work we study the associated polyhedra for some families of split graphs: headless spiders and complete suns. We provide the according linear relaxations, discuss their combinatorial structure, and demonstrate how the associated polyhedra can be entirely described or polyhedral arguments can be applied to find minimum identifying codes for special split graphs. We discuss further lines of research in order to apply similar techniques to obtain strong lower bounds stemming from linear relaxations of the identifying code polyhedron, enhanced by suitable cutting planes to be used in a B&C framework.","palabras_clave":"IDENTIFYING CODE PROBLEM|POLYHEDRAL APPROACH|SPLIT GRAPH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":211,"idioma_id":4,"titulo":"Geographic vectors of human mobility in the Andes (34-36°S): comparative analysis of 'minor' obsidian sources","resumen":"We have carried out longterm comparative in a wide latitudinal range of the Andes (29° to 37° south), encompassing selected areas that differ in ecology and biogeography. This latitudinal span shows diversity in subsistence modes across time and space, ranging from Andean agropastoralists in the north to Patagonian huntergatherers in the south. As part of this research, a program of geochemical and archaeological characterization of obsidian sources and distributions is being developed, attempting to assess connections between different biogeographical contexts. This work has allowed recognizing five Andean sources with different geological and topographical properties. Stemming from this macroregional endeavor, in this paper we present a comparative analysis of the human use of two sources that, based on the intensity of human use on a macroregional scale, can be interpreted as 'minor' sources.This study is framed on GIS leastcost models for human circulation across rugged landscapes allowing access to the sources. We assess temporal and spatial patterns of human occupation of highaltitude Andean deserts and neighboring areas comparing two of obsidian sources: Laguna del Diamante (34° S) and Cerro Huenul (36° S). The results indicate the existence of asymmetric distributions, particularly in the case of Laguna del Diamante. This evidence is used to assess the existence of dominant geographic vectors of human access and use of the Andean highlands. On this basis, we explore the analytical potential of minor sources for the study of human biogeography and organization of technology.","palabras_clave":"Geoquímica de obsidianas|Biogeografía humana|Arqueología comparativa|Andes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":221,"idioma_id":5,"titulo":"Biofumigation with Brassica juncea and Sinapis alba in vitro action to the growth of Trichderma and Azospirillum brasilense","resumen":"Biofumigation with Brassica juncea and Sinapis alba in vitro action to the growth of Trichderma and Azospirillum brasilense.  Se investigo el efecto de la biofumigación con Brassica y Sinapis sobre microorganismos benéficos presentes en un suelo agrícola","palabras_clave":"BIOFULMIGACION|BRASSICA|SINAPIS|TRICHODERMA-AZOSPIRILLUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":225,"idioma_id":4,"titulo":"Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations  and reduces DMH-associated colorectal cancer","resumen":"Chronic intestinal inflammation alters host physiology and could lead to colorectal cancer (CRC). Many studies have shown the efficiency of probiotics in the prevention and\/or treatment of Inflammatory Bowel Diseases (IBD). We have reported previously the beneficial effects of Lactobacillus casei BL23 in different murine models of intestinal inflammation. Moreover, there is emerging on the potential beneficial effects of probiotics in CRC. Here we thus explored the effects of L. casei BL23 on CRC and also on another non-intestinal cancer model. Our results show that oral treatment with this probiotic anti-inflammatory bacterium modulates host immune responses, significantly reduced the tumour volume in the HPV-tumor model and protected mice against 1,2-dimethylhydrazine (DMH)-induced CRC. Indeed, L. casei BL23-treated splenocytes showed significantly higher levels of Th17 and lower levels of Treg T-cell percentages than PBS-treated control cells. However, at the intestinal level, an anti-inflammatory response with increased IL-10 and decreased recruitment of macrophages accompanied the anti-tumor effect in probiotic administered mice. These results confirm the host immunomodulatory properties of L. casei BL23 that can exert benefices against tumors not only at the intestinal level but also systemically.","palabras_clave":"COLORECTAL CANCER|INFLAMMATION|LACTIC ACID BACTERIA|IMMUNOMODULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":226,"idioma_id":4,"titulo":"Kinetics of hydrogen evolution reaction on nickel modified by spontaneous Ru deposition: A rotating disk electrode and impedance spectroscopy approach","resumen":"The kinetics of hydrogen evolution reaction (HER) was studied on nickel catalysts prepared using a ruthenium spontaneous deposition method. Rotating disk electrode (RDE) and electrochemical impedance spectroscopy (EIS) were used for electrochemical characterization, and the results of the analysis were compared with pure nickel catalysts. Three different deposition times were used to obtain different ruthenium loads and to analyze their impact on the electrocatalytic activity. Kinetic and thermodynamic parameters of the reaction were obtained for freshly synthesized catalysts, and also for catalysts aged using a short chronoamperometric procedure. The presence of ruthenium was found to shift the reaction onset potential up to about 160 mV to lower overpotentials comparing with pure nickel, which not increases after ageing, showing a substantial improvement in catalytic activity. Results of Tafel and Koutecky-Levich (K-L) analysis have shown that the HER rate determining step is the Volmer reaction with Langmuir type adsorption for both, Ni\/Ru fresh and aged catalysts.","palabras_clave":"Hydrogen generation| Impedance spectroscopy|Nickel\/ruthenium|Rotating disk electrode|Spontaneous deposition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":236,"idioma_id":4,"titulo":"Mountain invasions on the way: are there climatic constraints for the expansion of alien woody species along an elevation gradient in Argentina?","resumen":"Aims: Increasing evidence worldwide indicates that high mountain regions are not immune to invasion by alien plants. Here, we aimed to address whether three major woody invaders of low-mountain areas are constrained to lower altitudes due to climatic restrictions or just by low propagule pressure. We hypothesize that the increas- ing climatic harshness towards higher altitudes restricts seedling establishment and survival of these woody invaders and thus their potential for altitudinal expansion.Methods: the study was carried out in the sierras grandes mountains, Córdoba, central argentina (32º50&#8242;s, 64º90&#8242;W). We addressed the hypothesis with an experimental approach, dissociating the effect of the environmental gradient from that of propagule pres- sure, by discarding the influence of seed sources. We translocated seeds of Gleditsia triacanthos l., Ligustrum lucidum W.t. aiton. and Pyracantha angustifolia (Franch.) C.K.schneid. along the altitudinal gradient (from 900 to 2700 m asl). seven sites were selected along the altitudinal gradient, spaced every 200?400 m. three plots (4 × 4 m) were selected within each altitudinal site and excluded from livestock; 100 seeds of each species were sown within each plot (6300 seeds in total). seedling emergence and survival was moni- tored during two growing seasons and soil temperature and mois- ture was recorded as well. the field experiment was complemented with lab assays. Important Findings: Climate along this altitudinal gradient might be least restrictive at mid elevations, as a result of the intersection between opposite soil tempera- ture and moisture gradients. sown seeds germinated along the whole altitudinal gradient and seedlings successfully established and over- wintered well above their current altitudinal distribution (up to 2200 m for Ligustrum and 2400 m for Gleditsia and Pyracantha). additional lab assays confirmed field patterns and gave some insights into con- trasting regeneration strategies between these invaders that might help to overcome stochastic environmental constraints in the germination stage. overall, seedlings of three major woody invaders of low-moun- tain woodlands can establish and survive at higher elevations than their current distribution. In contrast to natives, they seem to be broad climate tolerators, rather than specialized stress tolerators, capable of germinating and growing across a wide elevational range. While long- term experiments might be necessary to fully address the potentials for altitudinal expansion, out results on early lifecycle stage suggest that the invaders studied here would have mostly a dispersal barrier rather than a climate barrier to establish in the upper belt of the sierras grandes.","palabras_clave":"altitudinal gradient|exotic species|Gleditsia triacanthos|Ligustrum lucidum|Pyracantha angustifolia|seedling establishment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":237,"idioma_id":4,"titulo":"Changes in lithic technology and environment in Southern continental Patagonia: the Chico and Santa Cruz river basins","resumen":"Changes in artifact technology and environment during the Holocene are documented, within an organization of technology framework, for three different regions of Patagonia. Possible relationships between environmental changes and changes in artifact technology are explored. We examine past vegetation and geomorphic evidence of Holocene climate conditions in the three regions studied and what technologies were used during particular wetter and drier intervals. Our results suggest that many of the observed changes in technology use occurred at times of rapid climate change, particularly towards much drier conditions.","palabras_clave":"ENVIRONMENT|LYTHIC TECHNOLOGY|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":239,"idioma_id":4,"titulo":"Basement composition and basin geometry controls on upper-crustal deformation in the Southern Central Andes (30-36°S)","resumen":"Deformation and uplift in the Andes are a result of the subduction of the Nazca plate below South America. The deformation shows variations in structural style and shortening along and across the strike of the orogen, as a result of the dynamics of the subduction system and the features of the upper plate. In thiswork,we analyse the development of thin-skinned and thick-skinned fold and thrust belts in the Southern Central Andes (30?36° S). The pre-Andean history of the area determined the formation of different basement domains with distinct lithological compositions, as a result of terrane accretions during Palaeozoic time, the development of a widespread Permo-Triassic magmatic province and long-lasting arc activity. Basin development during Palaeozoic and Mesozoic times produced thick sedimentary successions in different parts of the study area. Based on estimations of strength for the different basement and sedimentary rocks, calculated using geophysical estimates of rock physical properties, we propose that the contrast in strength between basement and cover is the main control on structural style (thin- v. thick-skinned) and across-strike localization of shortening in the study area.","palabras_clave":"Subduction orogenesis|thin-skinned deformation|thick-skinned deformation|rock strength"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":241,"idioma_id":4,"titulo":"The interfacial properties of the peptide polybia-mp1 and its interaction with DPPC are modulated by lateral electrostatic attractions","resumen":"Polybia-MP1 (IDWKKLLDAAKQIL-NH2), extracted from the Brazilian wasp Polybia paulista, exhibits a broad-spectrum bactericidal activity without being hemolytic and cytotoxic. In the present study, we analyzed the surface properties of the peptide and its interaction with DPPC in Langmuir monolayers. Polybia-MP1 formed stable monolayers, with lateral areas and surface potential values suggesting mostly &#61537;-helical structure. The peptide occupied smaller areas on pure water compared to salt solutions as well as on acidic and basic ones. Furthermore, only on pure water, DPPC and MP1 co-crystalized forming branched domains, while on subphases with high salt concentrations or at acidic or basic conditions, the peptide was excluded from the domains. The results indicate that, on pure water, attractive electrostatic interactions between peptides were present, and the peptides were able to get closer to each other and to interact with DPPC. We suggest that the residues responsible of the peptide-peptide attraction are the anionic aspartic acids and the cationic lysines, which form a salt bridge, leading to oriented interactions in the crystal and thereby to branched domains. For this peptide, the balance between total attractive and repulsive interactions may be finely tuned by the aqueous ionic strength and pH. Since the described effect is related with lysines and aspartic acids, similar effects may also happen in other peptides with similar sequences.","palabras_clave":"PEPTIDE-PEPTIDE LATERAL INTERACTION|LANGMUIR MONOLAYER|DOMAIN SHAPE|MEMBRANE ELECTROSTATIC INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":242,"idioma_id":4,"titulo":"A new species of philometrid parasite (Nematoda, Philometridae) and histopathological lesions in juvenile whitemouth croakers, Micropogonias furnieri (Desmarest)","resumen":"A new species of nematode parasite, Philometroides tahieli sp. nov. is described. The juvenile white-mouth croaker, Micropogonias furnieri Desmarest,was collected during the spring and summer of the years 2008?2010 from estuarial environments of Argentina. During investigations of the parasite fauna a nematode encapsulated in the musculature of the operculum was found. The nematodes were removed from the host?s muscle tissue and slide-mounted in lactophenol solution to clarify the specimens. Opercular muscle with the parasite was processed for histopathological examination. Philometroides tahieli sp. nov. can be identified by the location of gravid females in the host, presence of anterior oesophageal bulb, bosses on the surface of the body, presence of transversal mounds and 14 cephalic papillae in two circular rows. The pathogenicity of the parasite is low in the natural environment, but lesions are consistent with a chronic process. The appearance of caseous necrosis suggests the presence of a locally acting substance. Philometroides tahieli sp. nov. is the second species of this genera described from brackish waters, and since the discovery of Philometroides maplestoni in 1928, is the first species of this genus recorded for South America.","palabras_clave":"caseous necrosis,|histopathological study,|Micropogonias furnieri,|Philometroides."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":246,"idioma_id":4,"titulo":"Growth pattern and changes in abundance of the endangered bat star Asterina stellifera","resumen":"The sea star Asterina stellifera has declined during the last decade and is currently abundant only in the southern limit of its former range. We surveyed this population over 5 years to model individual growth and explore the relationship of changes in local abundance with variation in environmental factors and the reproductive status of individuals. Our results show that A. stellifera is a species with slow growth and a relatively long lifespan. Contrary to expectations for temperate species, growth rates were fairly constant through the year and therefore models including seasonal oscillations were inappropriate. The abundance of this species increased significantly from early spring to early summer, likely due to augmented activity and small-scale aggregation during the reproductive season that affected our estimates of abundance. No significant recruitment occurred during the 5 years studied. The lack of recruitment during long periods and the slow individual growth rates make A. stellifera particularly vulnerable to local extinction. This study was performed prior to the arrival in the study area of the invasive kelp Undaria pinnatifida and side-gilled sea slug Pleurobranchaea maculata, species that threaten the community structure where A. stellifera lives. Therefore, the information reported here will be essential to assessing the impacts of these exotic species on this sea star population.","palabras_clave":"Somatic Growth|Asteroidea|endangered species|population dynamics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":248,"idioma_id":4,"titulo":"SMOS-MIRAS level 2 soil moisture product evaluation in rainfed croplands of the Pampean Region of Argentina","resumen":"A field campaign was carried out to evaluate the Soil Moisture (SM) MIR_SMUDP2 product (v5.51) generated from data of the Microwave Imaging Radiometer using Aperture Synthesis (MIRAS) aboard the Soil Moisture and Ocean Salinity (SMOS) mission. The study area was the Pampean Region of Argentina, which was selected because it is a vast area of flatlands containing quite homogeneous rainfed croplands considered SMOS nominal land uses. Transects of ground hand-held SM measurements were performed using ThetaProbe ML2x probes within four ISEA grid SMOS nodes, where permanent SM stations are located. The campaign results showed a negative bias of -0.02 m3m&#8722;3 between concurrent SMOS data and ground SM measurements, which means a slight SMOS-MIRAS underestimation, and a standard deviation of ± 0.06 m3m&#8722;3. Additionally, a good correlation was obtained between the hand-held SM measurements taken during the campaign and the permanent SM station data within a node, which pointed out that the station data could be used as reference data to evaluate the SMOS product over a longer temporal period on a continuous basis. SMOS-retrieved data were also compared with station mean SM values from June 2012 to December 2014. A general SMOS underestimation of -0.05 m3m&#8722;3 was observed in this case, with a standard deviation of ± 0.04 m3m&#8722;3 , which yields an uncertainty of ±0.07 m3m-3 for the SMOS product.","palabras_clave":"Soil moisture|Soil measurements|Uncertainty|Microwave radiometry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":250,"idioma_id":4,"titulo":"A ptsP deficiency in PGPR Pseudomonas fluorescens SF39a affects bacteriocin production and bacterial fitness in the wheat rhizosphere","resumen":"Pseudomonas fluorescens SF39a is a plant-growth-promoting bacterium isolated from wheat rhizosphere. In this report, we demonstrate thatthis native strain secretes bacteriocins that inhibit growth of phytopathogenic strains of the genera Pseudomonas and Xanthomonas. An S-typepyocin gene was detected in the genome of strain SF39a and named pys. A non-polar pys::Km mutant was constructed. The bacteriocin productionwas impaired in this mutant.To identify genes involved in bacteriocin regulation, random transposon mutagenesis was carried out. A miniTn5Km1 mutant, called P.fluorescens SF39a-451, showed strongly reduced bacteriocin production. This phenotype was caused by inactivation of the ptsP gene whichencodes a phosphoenolpyruvate phosphotransferase (EINtr) of the nitrogen-related phosphotransferase system (PTSNtr). In addition, this mutantshowed a decrease in biofilm formation and protease production, and an increase in surface motility and pyoverdine production compared withthe wild-type strain. Moreover, we investigated the ability of strain SF39a-451 to colonize the wheat rhizosphere under greenhouse conditions.Interestingly, the mutant was less competitive than the wild-type strain in the rhizosphere. To our knowledge, this study provides the firstevidence of both the relevance of the ptsP gene in bacteriocin production and functional characterization of a pyocin S in P. fluorescens.","palabras_clave":"REGULATION|BACTERIOCIN|PGPR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":256,"idioma_id":5,"titulo":"Pan frances fortificado con sales de calcio: propiedades reologicas y de panificación","resumen":"Conocida la importancia de la ingesta de calcio y la faltade acceso a este nutriente por gran parte de la población,se ensayó la formulación de panes fortificados condiferentes sales de calcio con el fin de facilitar el accesode las personas a dicho mineral a través de un alimentoeconómico y de consumo masivo. En general, los panesfortificados con lactato de calcio presentaron variosatributos negativos, tales como menor volumen específico,mayor dureza y gomosidad y sabor metálico. En cambio, la mayoría de las características de los panesfortificados con citrato de calcio y carbonato de calciofueron similares a los controles. Interesantemente, algunosde los atributos de los panes fortificados con carbonatode calcio fueron superiores a los controles. En consecuencia,la fuente de calcio más recomendable para lafortificación del pan francés sería el carbonato de calcio.","palabras_clave":"Pan |Calcio"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":261,"idioma_id":4,"titulo":"Synthesis and pharmaceutical properties of N-acyloxymethyl prodrugs of Allop with potential anti-trypanosomal activity","resumen":"We report herein the synthesis, and the physicochemical and pharmacokinetic properties of N-acyloxymethyl prodrugs of allopurinol (Allop) (2a-f). Allop is a compound with activity against Trypanosoma cruzi, a causative agent of Chagas disease. Its pathology leads to a huge number of infections and deaths per year, because in addition to many sufferers only having limited access to health services only an inefficient chemotherapy is available. Relevant pharmaceutical properties (pKa, stability, solubility, lipophilicity, in vitro permeability, binding protein, xanthine oxidase binding) were also determined. The results obtained showed that derivatives behave as prodrugs of Allop, since they exhibit improved physicochemical and pharmacokinetic properties relative to their precursor. This behavior turns these compounds into active reservoirs of Allop, and reduces its unfavorable characteristics, so 2a-f compounds are excellent candidates for the treatment of Chagas disease. This work is therefore an important contribution leading to the suppression of Chagas disease.","palabras_clave":"Chagas disease|N-acyloxymethyl prodrugs|physicochemical and pharmacokinetic properties|Trypanosoma cruzi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":269,"idioma_id":4,"titulo":"Spatio-temporal analysis of the role of faecal depositions in aggregation behaviour of the triatomine Rhodnius prolixus","resumen":"Several triatomine species aggregate over conspecific faeces. In some domestic species, this behaviour might help individuals to find their refuges because faeces are usually deposited around such places. In the present study, the defaecation behaviour of the triatomine bug Rhodnius prolixus Stål (Heteroptera: Reduviidae) is described in detail, including the temporal pattern of aggregation of nymphs with different nutritional status to faeces of different post&#8208;deposition times. First, using an experimental arena, the spatial distribution of faecal deposition spots released by nymphs, in relation to the position of a refuge, is recorded. The results show that a high density of faeces is deposited inside the refuge, and few and distant spots, in relation to the position of the refuge, are dropped outside. Second, whether the aggregation behaviour of nymphs is altered by the time elapsed from deposition of the faeces, and by the nutritional status of the insects, is determined. The post&#8208;deposition time of the faeces strongly modulates the aggregation response of the insects. Differences in aggregation between fed and unfed insects are only marginally significant; however, different temporal windows of aggregation according to the post&#8208;deposition times of the faeces are demonstrated between bugs of different nutritional status. The results are discussed with respect to the relevance of the aggregation signal in relation to the characteristics of the natural refuges used by R. prolixus.","palabras_clave":"RHODNIUS PROLIXUS|AGGREGATION|DEFAECATION PATTERN|REFUGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":270,"idioma_id":4,"titulo":"Eryptosis is induced by hyperthermia in hereditary spherocytosis red blood cell","resumen":"In pediatric hereditary spherocytosis (HS) patients, anemiafrequently worsens throughout the course of infectiousdiseases, due either to increased hemolysis or to transitoryerythropoietic aplasia [1]. Hemolysis may be so importantthat even asymptomatic individuals can incidentally bediagnosed with HS during a hemolytic crisis secondary tofebrile disease [2]. It has been reported that HS children,especially those under 6 years old, frequently suffer hemolyticcrises triggered by viral infections [2?4]. These reports,along with usual clinical practice, suggest a potentialharmful effect of temperature on erythrocytes which mayaffect their survival.Increasing evidence is now available to demonstratethat erythrocytes can also undergo premature self-destructionthrough a mechanism known as eryptosis, whichshares several features with nucleated-cell apoptosis.Since this process may be accelerated by different environmentalfactors, we decided to study whether hyperthermia?per se? has a direct effect on HS erythrocytes","palabras_clave":"ANEMIA|ERYPTOSIS|HEREDITARY SPHEROCYTOSIS|HYPERTHERMIA|INTRACELLULAR CALCIUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":275,"idioma_id":4,"titulo":"NARRATIVES ABOUT THE PAST AND COGNITIVE POLYPHASIA:  REMEMBERING THE ARGENTINE ?CONQUEST OF THE DESERT?","resumen":"The Conquest of the Desert was a military campaign waged by the Argentine government against the indigenous population during the late 19th century. This period of national organization and territorial expansion involved the extermination of the native populations, with thousands being killed or sold to wealthy landowners. This article reports the findings from an ethnographic study conducted in a city founded by the military forces during this period, where nowadays descendants of the military men and the European immigrants live alongside the descendants of the original inhabitants. In observations of the symbolic resources of the city and in interviews and discussions with descendants of European and military men, we identified 2 distinct narratives about this historical process: a traditional account concerning the peaceful coexistence of colonizers and indigenous groups, and a revisionist account that emphasizes the genocide of indigenous groups and the looting of their lands by the Argentine military. We consider the juxtaposition of these 2 narratives as an expression of a state of cognitive polyphasia that allows Argentine people to espouse a 'politically correct' version of the past while, at the same time, denying the conflict between colonizers and indigenous groups. We submit that this juxtaposition serves to make it possible for them to cope with the collective guilt that arises in relation to their ancestors´ behavior, while at the same time delegitimizing ongoing indigenous claims about past injustices and the need for historical reparation.","palabras_clave":"COLLECTIVE MEMORY |HISTORICAL NARRATIVES|INTERGROUP CONFLICT|COGNITIVE POLYPHASIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":277,"idioma_id":4,"titulo":"Astrocyte plasticity induced by emotional stress: A new partner in psychiatric physiopathology?","resumen":"A growing body of evidence has demonstrated that astrocytes play a pivotal role in the normal functioning of the nervous system. This new conceptual framework has set the groundwork to be able to hypothesize that astrocytes could underlie signs and symptoms of mental diseases. Stress is a major risk factor in the etiology of several psychiatric diseases, such as anxiety disorders and depression. Hence, understanding the effects of stress on astrocytes and how these changes contribute to the development of psychiatric endophenotypes is crucial for both a better comprehension of mental illness and for potential targeted treatment of stress-related mental disorders. Here, we describe the currently used approaches and recent evidence showing astrocyte alterations induced by chronic and acute stress in animals. In addition, the relevance of these changes in stress-induced behavioral sequelae and human data linking astrocytes with neuropsychiatric disorders related to stress are also discussed. All together, the data indicate that astrocytes are also an important target of stress, with both chronic and acute stressors being able to alter the morphology or the expression of several astrocyte specific proteins in brain areas that are known to play a critical role in emotional processing, such as the prefrontal cortex, hippocampus and amygdala. Furthermore, different lines of evidences suggest that these changes may contribute, at less in part, to the behavioral consequences of stress.","palabras_clave":"Acute stress|Anxiety|Astrocytes|Plasticity|Chronic stress|Depression"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":279,"idioma_id":4,"titulo":"Amaranth proteins foaming properties: Film rheology and foam stability-Part 2.","resumen":"In this work the influence of pH and ionic strength on the stability of foams prepared with amaranth  protein isolate was analyzed. The behaviour observed was related to the physico-chemical and structural  changes undergone by amaranth protein as a result of those treatments. The results obtained show  that foams prepared at acidic pH were more stable than the corresponding to alkaline pH. At pH 2.0  the foams presented higher times and more volumes of drainage. This behaviour is consistent with the  characteristics of the interfacial film, which showed a higher viscoelasticity and a greater flexibility at  acidic pH than alkaline pH value, which in turn increased by increasing the concentration of proteins in  the foaming solution. It is also important to note that the presence of insoluble protein is not necessarily  detrimental to the properties of the foam. Detected changes in the characteristics of the interfacial film  as in the foam stability have been attributed to the increased unfolding, greater flexibility and net charge  of amaranth proteins at acidic conditions.","palabras_clave":"amaranth proteins|air-water interfaces|film rheology|foaming properties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":281,"idioma_id":4,"titulo":"The gas metallicity gradient and the star formation activity of disc galaxies","resumen":"We study oxygen abundance profiles of the gaseous disc components in simulated galaxies in a hierarchical universe. We analyse the disc metallicity gradients in relation to the stellar masses and star formation rates of the simulated galaxies. We find a trend that galaxies with low stellar masses have steeper metallicity gradients than galaxies with high stellar masses at z &#771; 0. We also detect that the gas-phase metallicity slopes and the specific star formation rate (sSFR) of our simulated disc galaxies are consistent with recently reported observations at z &#771; 0. Simulated galaxies with high stellar masses reproduce the observed relationship at all analysed red shifts and have an increasing contribution of discs with positive metallicity slopes with increasing red shift. Simulated galaxies with low stellar masses have a larger fraction of negative metallicity gradients with increasing red shift. Simulated galaxies with positive or very negative metallicity slopes exhibit disturbed morphologies and\/or have a close neighbour. We analyse the evolution of the slope of the oxygen profile and sSFR for a gas-rich galaxy-galaxy encounter, finding that this kind of event could generate either positive or negative gas-phase oxygen profiles depending on their state of evolution. Our results support claims that the determination of reliable metallicity gradients as a function of red shift is a key piece of information in understanding galaxy formation and setting constraints on the subgrid physics.","palabras_clave":"galaxy formation |chemical evolution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":284,"idioma_id":4,"titulo":"CNS myelin structural modification induced in vitro by Phospholipases A2","resumen":"AbstractMyelin is the self-stacked membrane surrounding axons; it is also the target of severalpathological and\/or neurodegenerative processes like multiple sclerosis. These processes involve, among others, the hydrolytic attack by phospholipases. In this work we describe the changes in isolated myelin structure after treatment with several secreted PLA2 (sPLA2), by using small angle x-ray scattering (SAXS) measurements. It was observed that myelin treated with all the tested sPLA2s (from cobra and bee venoms and from pig pancreas) preserved the lamellar structure but displayed an enlarged separation between membranes in certain zones. Additionally, the peak due to membrane asymmetry was clearly enhanced. The coherence length was also lower than the non-treated myelin, indicating increased disorder. These SAXS results were complemented by Langmuir film experiments to follow myelin monolayer hydrolysis at the air\/water interface by a decrease in electric surface potential at different surface pressures. All enzymes produced hydrolysis with no major qualitative difference between the isoforms tested.","palabras_clave":"Myelin|phospholipases|SAXS|demyelination|Langmuir monolayers"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":289,"idioma_id":4,"titulo":"Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse","resumen":"The low efficiency of interspecies somatic cell nuclear transfer (iSCNT) makes it necessary to investigate new strategies to improve embryonic developmental competence. Embryo aggregation has been successfully applied to improve cloning efficiency in mammals, but it remains unclear whether it could also be beneficial for iSCNT. In this study, we first compared the effect of embryo aggregation over in vitro development and blastocyst quality of porcine, bovine, and feline zona-free (ZF) parthenogenetic (PA) embryos to test the effects of embryo aggregation on species that were later used as enucleated oocytes donors in our iSCNT study. We then assessed whether embryo aggregation could improve the in vitro development of ZF equine iSCNT embryos after reconstruction with porcine, bovine, and feline ooplasm. Bovine- and porcine-aggregated PA blastocysts had significantly larger diameters compared with nonaggregated embryos. On the other hand, feline- and bovine-aggregated PA embryos had higher blastocyst cell number. Embryo aggregation of equine-equine SCNT was found to be beneficial for embryo development as we have previously reported, but the aggregation of three ZF reconstructed embryos did not improve embryo developmental rates on iSCNT. In vitro embryo development of nonaggregated iSCNT was predominantly arrested around the stage when transcriptional activation of the embryonic genome is reported to start on the embryo of the donor species. Nevertheless, independent of embryo aggregation, equine blastocyst-like structures could be obtained in our study using domestic feline-enucleated oocytes. Taken together, these results reported that embryo aggregation enhance in vitro PA embryo development and embryo quality but effects vary depending on the species. Embryo aggregation also improves, as expected, the in vitro embryo development of equine-equine SCNT embryos; however, we did not observe positive effects on equine iSCNT embryo development. Among oocytes from domestic animals tested in our study, the feline ooplasm might be the most appropriate recipient to partially allow preimplantation embryo development of iSCNT equine embryos.","palabras_clave":"Clonación|Equinos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":294,"idioma_id":4,"titulo":"A QM\/MM study of the molecular recognition site of bapineuzumab toward the amyloid-&#946; peptide isoforms","resumen":"The molecular mechanism of recognition of amyloid-beta (Ab) peptide isoforms by bapineuzumab was studied using a  quantum mechanics and molecular mechanics (QM\/MM) method. In this work, geometric optimisations were performed  using the ONIOM2 scheme (at B3LYP\/6-31G(d) amber)EE level) on the paratope of bapineuzumab together with the  different forms of Ab peptide (AbWT and AbN3(pE)). A comprehensive study of the interactions was also performed  through Quantum Theory of Atoms in Molecules (QTAIM). This allowed us to obtain a deep understanding of how this  antibody interacts with the amino acids of the Ab peptides. The description on the interactions between bapineuzumab and  the different forms of Ab peptides allow us to understand why the peptides that lack the two first residues (Asp1 and Ala2)  and begin with a pyroglutamate residue present low affinity for bapineuzumab. This basic structural information is useful for  a deeper understanding about the scope and limitations of bapineuzumab as a therapeutic agent for the AD.","palabras_clave":"ALZHEIMER|AMYLOID-BETA|BAPINEUZUMAB"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":304,"idioma_id":4,"titulo":"Quantitative weighted mixed weak-type inequalities for classical operators","resumen":"We improve on several mixed weak type inequalities both for the Hardy-Littlewoodmaximal function and for Calder´on-Zygmund operators. These type of inequalitieswere considered by Muckenhoupt and Wheeden and later on by Sawyer estimatingthe L1,&#8734;(uv) norm of v&#8722;1T(fv) for special cases. The emphasis is made in provingnew and more precise quantitative estimates involving the Ap or A&#8734; constants ofthe weights involved.","palabras_clave":"MAXIMAL OPERATORS|CALDERON-ZYGMUND OPERATORS|WEIGHTED ESTIMATES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":307,"idioma_id":5,"titulo":"Familias tradicionales y elites empresarias en Argentina: individuación y solidaridad en la construcción y sostén de las posiciones de privilegio","resumen":"&amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';mso-font-charset:78;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1791491579 18 0 131231 0;}@font-face{font-family:'Cambria Math';panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:'Verdana Bold Italic';panose-1:2 11 8 4 3 5 4 11 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-1593833729 1073750107 16 0 415 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;text-align:justify;text-justify:inter-ideograph;mso-pagination:widow-orphan;font-size:12.0pt;mso-bidi-font-size:10.0pt;font-family:'Times New Roman';mso-fareast-font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';mso-fareast-theme-font:minor-fareast;mso-bidi-font-family:'Verdana Bold Italic';}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:'Verdana Bold Italic';mso-ascii-font-family:'Verdana Bold Italic';mso-fareast-font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:'Verdana Bold Italic';mso-bidi-font-family:'Verdana Bold Italic';}@page WordSection1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&amp;gt;Este artículotiene por objeto comprender cómo se producen, mantienen y justifican lasposiciones de privilegio en la Argentina. A partir de dos investigaciones queabordaron el mundo empresario y 'la clase alta' de dicho país, sostiene que enuna configuración donde el imaginario igualitario es central y donde no haycanales formales de acceso a las posiciones de elite, el trabajo activo deconstrucción, mantenimiento y justificación del lugar se vuelve ineludible. Elartículo muestra que ese proceso involucra la articulación de dos lógicas: laexigencia de una individuación meritocrática y la producción de formas desolidaridad. Describe los sentidos heterogéneos que ambas asumen y los modos deresolver sus tensiones y contradicciones en el proceso de disputar y consolidaruna posición de elite. ","palabras_clave":"Familias tradicionales|Merito|Empresarios|Padrinazgo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":308,"idioma_id":5,"titulo":"LA COMUNICACIÓN CON ENFOQUE DE GÉNERO, HERRAMIENTA TEÓRICA Y ACCIÓN POLÍTICA. MEDIOS, AGENDA FEMINISTA Y PRÁCTICAS COMUNICACIONALES. EL CASO DE ARGENTINA","resumen":"Un balance de la agenda política feminista resulta indispensable a la hora de intentar un diagnóstico acerca de la articulación entre prácticas comunicacionales, medios de comunicación y feminismos en América Latina. La pregunta por la instalación de agenda vendrá en este caso de la mano de otras estrategias utilizadas por un enfoque de género en tanto categoría de análisis transversal a todo el proceso comunicativo: las agencias de género\/feministas y las redes de periodistas de género\/feministas. El género como categoría descriptiva, como categoría analítica y como categoría política será la herramienta para el análisis del caso de la prensa en Argentina donde haremos una especial mención al tratamiento de la violencia contra las mujeres.","palabras_clave":"GÉNERO|ACCIÓN POLÍTICA|MEDIOS DE COMUNICACIÓN |FEMINISMOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":313,"idioma_id":4,"titulo":"Ruminal bacterial community changes during adaptation of goats to fresh alfalfa forage","resumen":"Using culture-independent molecular approaches, we studied the bacterial population changes in the rumen of goats abruptly converted from alfalfa hay to fresh alfalfa diet. Administration of fresh forage with significantly increased soluble nitrogen and soluble protein nitrogen resulted in frothy bloat. Changes of the bacterial composition of rumen were monitored using DGGE analysis of 16S rDNA gene amplicons and quantitative PCR method. As the diet changed, the bacterial population of Bacteroidetes and &#947;-Proteobacteria decreased, even if animals have not shown signs of frothy bloat. The most severely bloated animals showed an increase of Bacteroidetes phylum. Lactobacillus\/Streptococcus group belonging to Firmicutes phylum decreased in response to transferring the animals from hay to a fresh forage-based diet, and did not achieve the values observed at the beginning of the experiment. In summary, changes in the diet and subsequent frothy bloat occurrence produce long-lasting changes in the structure of the microbial community and may be associated with a specific bacterial population belonging to the Bacteroidetes phylum.","palabras_clave":"BACTERIA|CREOLE GOATS|PCR-DGGE|qPCR|RUMEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":315,"idioma_id":5,"titulo":"Estudios citogenéticos y de contenido de ADN en Brasiliopuntia schulzii (Cactaceae)","resumen":"Se determinaron númerocromosómico, cariotipo, patrones de bandeo, localización de genes ribosómicos ycontenido de ADN nuclear, en dos poblaciones de Brasiliopuntia schulzii (Cactaceae, subfam. Opuntioideae). La especie resultó diploide (2n=22), presentó unafórmula cariotípica de 10 m + 1 sm. El bandeo cromosómico CMA\/DAPIreveló la presencia de un par cromosómico con una banda CMA+\/DAPI-asociada a NORs. Con la técnica de FISH se observó que los loci18-5,8-26Sfueron consistentes con los bloques CMA+\/DAPI-\/NORs. Laseñal para el gen 5S se localizó en el cuarto par m en una región telomérica. En las dos poblaciones el análisis decontenido de ADN reveló una mezcla de núcleos con tres picos de 2C, 4C y 8C. Esto indicaría un proceso deendopoliploidía en la especie. Los resultados reportados en este trabajo,combinados con las características morfológicas, indicarían que este géneroposee caracteres basales, concordando con los análisis filogenéticos de lasubfamilia.","palabras_clave":"BRASILIOPUNTIA|CITOGENETICA|CONTENIDO DE ADN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":318,"idioma_id":4,"titulo":"Rolling Shutter Effect Aberration Compensation in Digital Holographic Microscopy","resumen":"Due to the sequential-readout nature of most CMOS sensors, each row of the sensor array is exposed at a different time, resulting in the so-called rolling shutter effect that induces geometric distortion to the image if the video camera or the object moves during image acquisition. Particularly in digital holograms recording, while the sensor captures progressively each row of the hologram, interferometric fringes can oscillate due to external vibrations and\/or noises even when the object under study remains motionless. The sensor records each hologram row in different instants of these disturbances. As a final effect, phase information is corrupted, distorting the reconstructed holograms quality. We present a fast and simple method for compensating this effect based on image processing tools. The method is exemplified by holograms of microscopic biological static objects. Results encourage incorporating CMOS sensors over CCD in Digital Holographic Microscopy due to a better resolution and less expensive benefits.","palabras_clave":"CMOS|Digital Holographic Microcopy|Rolling Shutter Effect|Optical phase aberration"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":322,"idioma_id":4,"titulo":"MASS TRANSFER LIMITATIONS IN A MONOLITHIC REACTOR FOR THE CATALYTIC OXIDATION OF ETHANOL","resumen":"A theoretical study of a monolithic reactor for the catalytic oxidation of Volatile Organic Compounds (VOC) over manganese?copper mixed oxide catalysts is presented. An isothermal one-dimensional heterogeneous model is selected to account for the external (gas?solid) and internal (washcoat) mass transport limitations.The results reveal that under certain operating conditions, e.g. thick washcoats and high conversions of VOCs (high temperatures), the overall process rate is reduced by internal diffusion inside the catalyst. At high temperatures and\/or monoliths with poor interfacial areas, the overall process rate is limited by external mass transfer resistance to the catalyst. Proper quantification of both internal and external mass transfer resistances by means of a heterogeneous model can result in avoiding situations of incomplete VOC abatement under conditions of high VOC dilution.","palabras_clave":"Catalytic oxidation|Monolithic reactor|Heterogeneous model|ethanol"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":340,"idioma_id":5,"titulo":"'Formación Profesional e Inserción Laboral  en Artes, Diseño  y Humanidades. Estrategias para una articulación efectiva'","resumen":"Esta investigación es financiada por la UNCUYO mediante sus Programas I+D (período 2010-2014), e incluye las Facultades de Artes y Diseño y de Filosofía y Letras. Se aborda el diagnóstico y propuestas para contribuir a optimizar la relación formación profesional-inserción laboral en carreras de Artes, Diseño y Humanidades. La inserción laboral de egresados en disciplinas artísticas, tecnológicas y humanísticas sería diferente según cada carrera. Una hipótesis lo atribuye a la falta de ?cultura en las artes, el diseño y las humanidades? en el medio. Los resultados podrán transferirse a las unidades académicas consideradas en el estudio y a otras afines.","palabras_clave":"formación profesional|inserción laboral|acreditación|empleo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":341,"idioma_id":5,"titulo":"Modelo conceptual preliminar del sistema geotermal El Sauce (Salta) a partir de estudios de geoquímica de fluidos","resumen":"Si bien en el noroeste argentino existen numerosos sitios de interés para la explotación del recurso geotérmico, la mayoría de ellos sólo cuenta con estudios de reconocimiento. En esta contribución se presentan los resultados del estudio geoquímico de los fluidos relacionados al sistema geotermal El Sauce utilizados para construir un modelo conceptual preliminar, donde se describe el origen de los compuestos y las condiciones de P-T de los fluidos dentro del reservorio. La caracterización geoquímica se realizó mediante el muestreo directo de aguas y gases, y el posterior análisis de su composición química e isotópica. El reservorio geotermal, alojado en la Formación Yacoraite, adquiere una composición HCO3&#713;-Na+ por interacción agua-roca, favorecida por el aporte de CO2 cortical. Los gases atmosféricos disueltos en las aguas meteóricas que recargan el acuífero dominan la composición de la fase gaseosa. El acuífero profundo se mezcla con acuíferos salinos de composición Cl&#713;(SO42-)-Na+ (ubicados dentro del Subgrupo Metán) en su ascenso a superficie a través de las estructuras. Finalmente, el agua termal sufriría procesos de mezcla y enfriamiento con acuíferos superficiales fríos de composición HCO3&#713;-Ca2+\/Na+ y baja salinidad existentes dentro de depósitos cuaternarios. Considerando que el reservorio geotermal está a ~2,6 km de profundidad, el agua podría alcanzar la temperatura estimada mediante geotermómetros (~106 °C) en un gradiente geotérmico normal ~33 °C\/km. Esto último sumado a la isotopía de los gases permite desestimar la hipótesis propuesta en trabajos previos que asignaba un origen magmático tanto para los gases como para la anomalía de calor.","palabras_clave":"GEOQUIMICA DE AGUA Y GASES|RESERVORIO GEOTERMAL|CIRCULACION PROFUNDA|RECURSO GEOTERMICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":349,"idioma_id":4,"titulo":"Joint application of antimicrobial agents on microbial flora chilled   meat cattle. Use of mathematical models","resumen":"Objectives of this work were: i) to study the effect joint application of antimicrobial agents on microbial flora chilled meat cattle;ii) to model mathematically this microbial growth and iii) validate the models with own experimental results.Samples were irradiated with UVC light for 5 minutes and then were added a solution of oregano oil and lactic acid (1: 1).Untreated samples were considered as control.Gompertz model was appropriate to quantify microbial growth in all conditions studied. Meanwhile, Pseudomonas sp in treatedsamples showed no development, having to apply the linear regression model","palabras_clave":"beef|UVC light|essential oils|refrigeration|mathematical models"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":353,"idioma_id":4,"titulo":"TRENDS IN THE RESISTANCE PROFILES OF ACINETOBACTER BAUMANNII ENDEMIC CLONES IN A UNIVERSITY HOSPITAL OF ARGENTINA.","resumen":"A total of 925 Acinetobacter spp. isolates were collected from routine clinical samples of patients admitted tothe university hospital of Buenos Aires city during the period 2004?2012. From this collection, 129 isolatesidentified as Acinetobacter baumannii were selected for molecular studies. Minimal inhibitory concentrations(MICs) of antimicrobials were determined by agar dilution method. Colistin (COL) heteroresistance wasinvestigated by means of population analysis studies. PCR-based methods were used for epidemiologicalanalysis and for the screening of carbapenemases and the blatetB gene. We have observed a steady rise in theMIC50 of imipenem (IMI)-resistant isolates and an increment in the presence of blaOXA-23-like gene (74?100%)as well. A rapid increasing rate of minocycline (MIN) resistance and a rise of the MIC50 of the resistant isolateshave been detected since the year 2008. All isolates harboured the tet (B) gene. An increase in the value of thetigecycline (TIG) MIC was seen from the year 2007 onwards. This loss of activity was observed among differentclones. A rise of COL heteroresistance from 46.4% in 2004 to 95% in 2012 was detected. During this period,COL consumption also increased (11.1-fold). However, COL resistance remained sporadic.","palabras_clave":"Acinetobacter baumannii|colistin usage|heteroresistance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":370,"idioma_id":4,"titulo":"Effect of Montmorillonite on the Crystallization and Thermal Degradation of Poly(propylene-co-ethylene-co-1-butene) Nanocomposites","resumen":"Publicación on-line: Julio-2016Publicación impresa: 30(6) 741-761 (2017)Resumen: Polymeric nanocomposites based onpoly(propylene-co-ethylene-co-1-butene) (PEBC) were elaborated by melt mixingusing an organophilic montmorillonite (o-MMT), and maleated PEBC (PEBCg) ascompatibilizer. The effect of clay concentration, PEBCg:o-MMT ratio, and graftingdegree of the compatibilizer, were consider. X-ray diffraction and scanningelectron microscopy show formation of partially intercalated structures in allcompatibilized composites with well distributed small tactoids. According tothe differential scanning calorimetry results, the anhydride groups of thecompatibilizer have a marginal nucleating effect, while the o-MMT causes aslight decrease in the crystallization temperature of the polymer. PEBCpresents the largest activation energy of crystallization, Ea, while the compositesshow lower Ea than their matrices. It is alsoobserved that the rate of degradation of PEBC is not affected by the presenceof PEBCg. The nanoclay, on the other hand, retards the decomposition process ofthe polymeric matrix in about 40°C and augments its rate of degradationapproximately 4 times.","palabras_clave":"NANOCOMPOSITES|POLYPROPYLENE COPOLYMERS|MONTMORILLONITE|THERMAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":374,"idioma_id":4,"titulo":"Growth, carcass and meat quality traits in beef from Angus, Hereford and cross-breed grazing steers, and their association with SNPs in genes related to fat deposition metabolism","resumen":"Grazing steers from Angus and Hereford breeds, their cross-breeds and a three-way cross-breed (Limousin × Angus?Hereford) were measured for growth, carcass and meat quality traits. Breed effects were studied, and the association of SNPs with fat deposition and fatty acid (FA) composition (leptin, melanocortin-4 receptor, stearoyl-CoA desaturase, FA synthase and thyroglobulin) was tested. Limousin cross-breed showed the greatest final body weight, ultrasound rib eye area, dressing percentage, carcass and leg length, and the lowest backfat thickness and intramuscular fat content. Genetic groups had similar pH, shear force, cooking loss, L* and b* and n-6:n-3 ratio. Meat from 1\/2-Angus presented greater a* than Limousin cross-breed. Whereas Angus had the highest total SFA content, Hereford had the lowest total SFA and the highest total MUFA. Limousin cross-breed had greater content of several individual PUFAs, total PUFA, n-6 and n-3 FA than Angus and 1\/2-Angus. Leptin and FA synthase were associated with some FAs, supporting their influence over fat metabolism for grazing animals.","palabras_clave":"Angus-Hereford cross-breeds|growth|carcass|meat quality| fatty acids|single nucleotide polymorphisms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":376,"idioma_id":4,"titulo":"The palynological record across the Ordovician\/Silurian boundary in the Cordillera Oriental, Central Andean Basin, northwestern Argentina","resumen":"The Hirnantian ?glacial horizon? has been recognized across the Central Andean Basin(Puna, Cordillera Oriental, Sierras Subandinas and Sistema de Santa Bárbara) innorthwestern Argentina. It is represented by glaciofluvial to glaciomarine facies whichunconformably overly Lower to Middle Ordovician stratigraphic units. In the Caspalá area,in the eastern margin of the Cordillera Oriental, the glacial deposits are assigned to theCaspalá Formation. The Lower Silurian postglacial deposits of the Lipeón Formationsharply truncate the underlying Caspalá Formation. Miospores, chitinozoans and acritarchshave been recorded across the Ordovician\/Silurian boundary. Marine palynomorphs dominate the studied section. The Late Ordovician miospore assemblage is fairly diverse.It contains permanent tetrads and dyads, spores physically separated from cryptosporepolyads, laevigate and ornamented hilate spores, and trilete spores. The trilete sporesAmbitisporites avitus, Aneurospora? sp., Chelinospora cf. C. prisca and Leiotriletes spp.occur in the Caspalá Formation together with chitinozoans dated as early to late Katian. Ifland-derived palynomorphs were considered autochthonous, their age would be Hirnantian.The trilete spores of the Caspalá Formation constitute their oldest record in South America,representing the advent of vascular plants in the region. The Lipeón Formation yieldedTelychian trilete spores dated by chitinozoans, constituting the earliest evidence of Silurianvascular plants of Argentina. The diversity and abundance of miospores decrease in the Lipeón Formation in accordance to the disappearance of terrestrial ecosystems due to theglobal transgression after the melting of the Hirnantian glaciers. Acritarchs in both theCaspalá and the Lipeón Formation support the chitinozoan dating. Whereas chitinozoansand acritarchs show affinities with Gondwanan and peri-Gondwanan regions, the studiedmiospores confirm the cosmopolitism of Late Ordovician-earliest Silurian microfloras. The new miospore data, particularly those related to the incoming and evolution of hilate\/triletespores, question previous palaeogeographic and palaeoclimatic interpretations about theorigin and adaptive radiation of vascular land plants.","palabras_clave":"MIOSPORES|CHITINOZOANS|ACRITARCHS|TERRESTRIALIZATION|ORDOVICIAN\/SILURIAN BOUNDARY|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":380,"idioma_id":4,"titulo":"Gravimetric determination of the continental ? oceanic boundary of the Argentine continental margin (from 36°S to 50°S).","resumen":"This paper presents the gravimetricanalysis together with seismic data as an integral application in order toidentify the continental?oceanic crust boundary (COB) of the Argentine continentalmargin from 36&#9702;S to 50&#9702;S in a continuousway. The gravimetric and seismic data are made up of large grids of dataobtained from satellite altimetry and marine research. The methodology consistsof three distinct methods: (i) the application of enhancement techniques togravimetric anomalies, (ii) the calculation of crustal thinning from 3-Dgravity inversion modeling of the crust?mantle discontinuity and (iii) 2-Dgravimetric modeling supported by multichannel reflection and refractionseismic profiles. In the first method, the analytic signal, Theta map, and tiltangle and its horizontal derivative were applied. In the second method, crustalthickness was obtained as the difference in the depths of the crystallinebasement and the crust?mantle discontinuity; thelatter was obtained via gravimetric inversion. Finally, 2-D modeling wasperformed from free-air anomalies in two representative sections by consideringas restriction surfaces those coming from the interpretation of seismic data.The results of the joint application of enhancement techniques and 2-D and 3-D modelinghave enabled continuous interpretation of the COB. In this study, the COB wasdetermined continuously from the integration of 2-D profiles of the enhancementtechniques, taking account of crustal thickness and performing 2-D gravimetric modeling.The modeling technique was complemented by regional studies integrated withmultichannel seismic reflection and seismic refraction lines, resulting inconsistent enhancement techniques.","palabras_clave":"plataforma|contacto|transición|Métodos Potenciales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":381,"idioma_id":4,"titulo":"Liquid chromatography coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry and post-column addition of metal salt solutions as a powerful tool for the metabolic profiling of Fusarium oxysporum","resumen":"Fusarium oxysporum L11 is a non-pathogenic soil-borne fungal strain that yielded an extract that showed antifungal activity against phytopathogens. In this study, reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to different atmospheric pressure ionization sources-quadrupole-time-of-flight mass spectrometry (API-QTOF-MS) was applied for the comprehensive profiling of the metabolites from the extract. The employed sources were electrospray (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). Post-column addition of metal solutions of Ca, Cu and Zn(II) was also tested using ESI.A total of 137 compounds were identified or tentatively identified by matching their accurate mass signals, suggested molecular formulae and MS\/MS analysis with previously reported data. Some compounds were isolated and identified by NMR. The extract was rich in cyclic peptides like cyclosporins, diketopiperazines and sansalvamides, most of which were new, and are reported here for the first time. The use of post-column addition of metals resulted in a useful strategy for the discrimination of compound classes since specific adducts were observed for the different compound families. This technique also allowed the screening for compounds with metal binding properties. Thus, the applied methodology is a useful choice for the metabolic profiling of extracts and also for the selection of metabolites with potential biological activities related to interactions with metal ions.","palabras_clave":"Fusarium oxysporum"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":382,"idioma_id":5,"titulo":"Articulaciones comunidad-universidad para la gestión de programas y políticas sociales contextuadas","resumen":"En este trabajo presentamos tres investigaciones interdisciplinarias que pretendieron identificar necesidades de la población respecto a atención y cuidados de personas mayores para realizar propuestas de intervención contextuadas articulando regulaciones, saberes y prácticas de diferentes sectores, organizaciones y actores sociales. Los resultados emergentes permiten reflexionar sobre la función social de la universidad y la producción de conocimientos científicos conformes a los saberes y prácticas de los diferentes actores sociales. Aunque las lógicas de la producción y validación académico-científica son diferentes a las de los procesos de salud-enfermedad-atención y gestión, necesitan pensarse, trabajarse y articularse para garantizar el derecho pleno de cada persona y la responsabilidad que ello conlleva. Esto implica pensar las acciones desde una perspectiva de derecho y de deberes de todos y cada uno de los niveles, sectores, instituciones y actores involucrados.","palabras_clave":"Políticas sociales|Universidad|Salud Comunitaria"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":385,"idioma_id":5,"titulo":"ESTUDIO ARQUEOBOTÁNICO DE LOS RESIDUOS ORGÁNICOS ADHERIDOS EN ALFARERÍAS PREHISPÁNICAS DE LA CUENCA DEL PARANÁ MEDIO","resumen":"El estudio de microrrestos vegetales constituye a nivel nacional una línea de investigación relativamente reciente en arqueología, y de gran potencial informativo para explorar las relaciones entre los grupos humanos y los recursos vegetales de su entorno. En el Nordeste argentino, los análisis de microrrestos vegetales han empezado a aplicarse en los últimos años en la cuenca del Paraná inferior, pero aún son muy escasos en el Paraná medio. Este trabajo presenta los primeros resultados del análisis de microrrestos silíceos y almidonosos en muestras cerámicas con residuos orgánicos, procedentes de sitios arqueológicos localizados en la margen izquierda de la cuenca del Paraná medio, en la provincia de Entre Ríos. Los datos obtenidos sugieren que los recipientes contuvieron calabaza (Cucurbita moschata), batata (Ipomea batatas) y probablemente maíz (Zea mays), así como también una presencia variable de gramíneas. Estos análisis aportan evidencias sobre la utilización de los recursos vegetales porparte de los grupos prehispánicos que ocuparon el área durante el Holoceno tardío, y contribuyen a discutir problemáticas regionales vinculadas con los patrones alimenticios de estos grupos.","palabras_clave":"ESTUDIOS ARQUEOBOTÁNICOS|MICRORRESTOS SILÍCEOSY ALMIDONOSOS|VASIJAS CERÁMICAS|CUENCA DEL PARANÁ MEDIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":390,"idioma_id":4,"titulo":"Changes of myoid and endothelial cells in the peritubular wall during contraction of the seminiferous tubule","resumen":"The wall of the seminiferous tubule in rodents consists of an inner layer of myoid cells covered by an outer layer of endothelial cells. Myoid cells are a type of smooth muscle cell containing &#945;-actin filaments arranged in two independent layers that contract when stimulated by endothelin-1. The irregular surface relief of the tubular wall is often considered a hallmark of contraction induced by a variety of stimuli. We examined morphological changes of the rat seminiferous tubule wall during contraction by a combination of light, confocal, transmission and scanning electron microscopy. During ET-1-induced contraction, myoid cells changed from a flat to a conical shape, but their actin filaments remained in independent layers. As a consequence of myoid cell contraction, the basement membrane became wavy, orientation of collagen fibers in the extracellular matrix was altered, and the endothelial cell layer became folded. To observe the basement of the myoid cell cone, the endothelial cell monolayer was removed by collagenase digestion prior to SEM study. In contracted tubules, it is possible to distinguish cell relief: myoid cells have large folds on the external surface oriented parallel to the tubular axis, whereas endothelial cells have numerous cytoplasmic projections facing the interstitium. The myoid cell cytoskeleton is unusual in that the actin filaments are arranged in two orthogonal layers, which adopt differing shapes during contraction with myoid cells becoming cone-shaped. This arrangement impacts on other components of the seminiferous tubule wall and affects the propulsion of the tubular contents to the rete testis.","palabras_clave":"ALPHA-ACTIN|ENDOTHELIN-1|PERITUBULAR MYOID CELL|SEMINIFEROUS TUBULE|TESTIS ULTRASTRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":397,"idioma_id":5,"titulo":"COMPARACIÓN DE MÉTODOS PARA PRONOSTICAR EL ÍNDICE DE  TEMPERATURA Y HUMEDAD (ITH) DIARIO EN EL DEPARTAMENTO  NOGOYÁ, ENTRE RÍOS","resumen":"El ITH, un índice que combina las variables temperatura y humedad, se utilizapara determinar si los bovinos de leche se hallan en condiciones ambientales deconfort. Cuando el ITH supera el valor 72 el bovino puede entrar en estréscalórico lo que podría repercutir de manera negativa en la producción láctea.Los tamberos pueden implementar estrategias de manejo para reducir losefectos negativos del estrés calórico, por eso resulta relevante contar con unpronóstico del ITH que permita anticipar la ejecución de prácticas adecuadas.En el presente trabajo se proponen y evalúan dos metodologías para laestimación del ITH y la duración de los intervalos con ITH superior a 72 apartir de datos de temperatura del aire, con la finalidad de desarrollar unsistema de pronóstico para la localidad de Nogoyá, Entre Ríos. Lasmetodologías propuestas consisten en a) estimación del ITH a partir defunciones lineales ajustadas entre ITH y temperatura; y b) estimación del ITH apartir de la evolución horaria de la temperatura y de la humedad relativa,estimadas con una función senoidal. Se utilizaron datos disponibles de laestación meteorológica automática de Nogoyá de la Bolsa de Cereales de EntreRíos correspondientes al período septiembre 2014 ? marzo 2015. En el métodoa) se ajustaron dos funciones lineales para estimar el ITH máximo y mínimodiario a partir de la temperatura máxima y mínima diaria, respectivamente. Apartir de los valores estimados de ITH máximo y mínimo se aplicó un esquemade proporcionalidad para determinar las horas diarias con valores de ITHsuperiores a 72. En el método b) se utilizó una función senoidal para estimar laevolución diaria de la temperatura y la HR a partir de la temperatura máxima ymínima diaria, que se emplearon para el cálculo del ITH. Se determinó elintervalo en horas con ITH superiores a 72, realizándose también a escaladiaria. Los dos métodos propuestos fueron similares y adecuados para laestimación del ITH y la duración de los intervalos de tiempo con ITH superiora 72. El método a) resulta más sencillo de utilizar porque requiere menoscálculos que el método b) pero este último permite estimar además la duraciónde períodos de tiempo con ITH superior a 72 que exceden el intervalo diario.","palabras_clave":"ESTRÉS CALÓRICO|GANADERÍA|ÍNDICE|PRONÓSTICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":526,"idioma_id":4,"titulo":"Echinoderms in San Matias Gulf, Southwestern Atlantic Ocean","resumen":"Echinoderms are often of ecological importance in intertidal and subtidal waters, especially as predators and herbivores but also as prey. Several groups of echinoderms respond in a different way to environmental variables, contributing to some of the biodiversity patterns found along latitudinal gradient. This work listed the echinoderms species of San Matías Gulf surrounding the coast of Río Negro Province, and analyzed the current state of knowledge of this group by previous works, collection items of the Museo Argentino de Ciencias Naturales ?Bernardino Rivadavia? and from samples taken since 2009 to the present. A total of 35 species of echinoderms corresponding to four classes were recorded. The 34.29% corresponds to Asteroidea, 31.43% to Ophiuroidea, 20% to Echinoidea and 14.28% to Holothuroidea. Crinoidea has not been reported for San Matías Gulf. Asteroidea and Ophiuroidea were the most representative groups follow by Echinoidea and Holothuroidea, with only five species in the last class. About 30% of country?s species are present in Río Negro Province. This high number of species may be due to the heterogeneity of environments that are possible to find in San Matías Gulf and a transition zones between the Magellanic and Argentine biogeographic provinces that can provide particular physical and climatic characteristics explaining as consequence the faunal composition.","palabras_clave":"ECHINODERMS|BIOGEOGRAPHIC PROVINCES|SAN MATIAS GULF|SOUTHWESTERN ATLANTIC OCEAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":587,"idioma_id":4,"titulo":"Beyond admissibility: accepting cycles in argumentation with game protocols for cogency criteria","resumen":"In this article, we extend Dung´s formal approach from admissibility to less demanding extension semantics allowing arguments in cycles of attacks. We present an acceptance criterion leading to the characterization of three semantics called pairwise cogency , weak cogency and cyclic cogency . Particular game-theoretic protocols allow us to identify winning strategies with extensions in different semantics. Furthermore, an algorithmic characterization of those games exhibits clearly how self-attacking or in odd-length cycles of attack can be rationally managed beyond the limits of admissibility.","palabras_clave":"Argumentation|Artificial Intelligence|Defeasible Reasoning|Dialogue games"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":717,"idioma_id":4,"titulo":"Fleming-Viot selects the minimal quasi-stationary distribution: The Galton-Watson case","resumen":"Consider N particles moving independently, each one according to a subcritical continuous-time Galton-Watson process unless it hits 0, at which time it jumps instantaneously to the position of one of the other particles chosen uniformly at random. The resulting dynamics is called Fleming-Viot process. We show that for each N there exists a unique invariant measure for the Fleming-Viot process, and that its stationary empirical distribution converges, as N goes to infinity, to the minimal quasi-stationary distribution of the Galton-Watson process conditioned on non-extinction.","palabras_clave":"Fleming-Viot|Quasi-stationary distributions|particle systems|Selection principle"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":732,"idioma_id":4,"titulo":"Total cohomology of solvable Lie algebras and linear deformations","resumen":"Given a finite-dimensional Lie algebra g, let &Gamma;o(g) be the set of irreducible g-modules with non-vanishing cohomology. We prove that a gmodule V belongs to &Gamma;o(g) only if V is contained in the exterior algebra of the solvable radical s of g, showing in particular that &Gamma;o(g) is a finite set and we deduce that H&lowast;(g, V) is an L-module, where L is a fixed subgroup of the connected component of Aut(g) which contains a Levi factor. We describe &Gamma;o in some basic examples, including the Borel subalgebras, and we also determine &Gamma;o(sn) for an extension sn of the 2-dimensional abelian Lie algebra by the standard filiform Lie algebra fn. To this end, we described the cohomology of fn. We introduce the total cohomology of a Lie algebra g, as (formula presented) and we develop further the theory of linear deformations in order to prove that the total cohomology of a solvable Lie algebra is the cohomology of its nilpotent shadow. Actually we prove that s lies, in the variety of Lie algebras, in a linear subspace of dimension at least dim(s\/n)2, n being the nilradical of s, that contains the nilshadow of s and such that all its points have the same total cohomology.","palabras_clave":"Lie nilshadow|Lie cohomology|Solvable Lie algebras|Deformations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":878,"idioma_id":4,"titulo":"Diel locomotor activity and shelter use in the Patagonian catfish Hatcheria macraei under experimental conditions","resumen":"Diel locomotor activity and shelter use was experimentally evaluated in Hatcheria macraei under three different light regimens: natural and inverted photoperiods, and during constant darkness. The results demonstrate clear nocturnal activity and highlight the negative phototactic behaviour of H. macraei, proving that light is the external factor that triggers the seeking of shelter and the subsequent hiding action. Locomotor activity was maximal in darkness, intermediate at dawn and dusk, and minimal (maximum shelter use) in full light. During free-running experiments (i.e. constant darkness) H. macraei continued to exhibit the same diel pattern of resting (or hiding) and swimming as under natural light conditions. Nocturnal activity and negative phototactic behaviour appear to be important mechanisms which have enabled H. macraei to withstand the effects of salmonid introduction in loticenvironments where other native fishes have suffered negative effects.","palabras_clave":"Circadian rhythm \u0002  \u0002  \u0002  \u0002 |Artificial photoperiod|Hiding behaviour|Refuge|Swimming activity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":881,"idioma_id":4,"titulo":"Genetic variation for wheat spike fertility in cultivars and early breeding materials","resumen":"Grain yield in bread wheat is often tightly associated with grain number\/m2. In turn, spike fertility (SF), i.e., the quotient between grain number and spike chaff dry weight, accounts for a great proportion of the variation in grain number among cultivars. In order to examine the potential use of SF as a breeding target, (1) variation for the trait was assessed in six datasets combining commercial cultivars under different environmental conditions, (2) trait heritability was estimated in a set of F1 hybrids derived from controlled crosses between cultivars with contrasting SF and (3) SF distribution pattern was analysed in two F2 segregating populations. Analysis of commercial cultivars revealed considerable variation for SF, under both optimal and sub-optimal conditions. In addition, genotypic variation was consistently larger than genotype × environment interaction variation in all datasets. Narrow sense heritability, estimated by the mid-parent-offspring regression of 20 F1 hybrids and their respective parents, was 0·63. Data from two F2 populations exhibited bell-shaped and symmetric frequency distributions of SF, with a SF mean intermediate between the parental values. Substantial transgressive segregation was detected in both F2 populations. In conclusion, SF appears to be a heritable trait with predominantly additive effects. This warrants further investigation on the feasibility of using SF as an early selection criterion in wheat breeding programs aimed at increasing grain yield.","palabras_clave":"BREEDING|GRAIN NUMBER|HERITABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":885,"idioma_id":4,"titulo":"Sufficient reductions in regressions with elliptically contoured inverse predictors","resumen":"-","palabras_clave":"sufficient reduction|exponential family"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":1370,"idioma_id":4,"titulo":"A Parental Care-Mating Dilemma? Potential Risks for Offspring in the Pholcid Spider When Egg-Carrying Females Accept Mating","resumen":"Many species face a trade-off between additional mating opportunities and the offspring benefits (viability, quality) provided by parental care. Female Holocnemus plucheispiders must abandon their egg-sac, which they otherwise carry with their chelicerae, tocopulate. This may involve risks for the offspring, such as predation and fungal infection.We assessed whether (1) males discriminate between egg-carrying females according to theegg development stage, (2) females are influenced by the egg development stage in regard to their proneness tomate, and (3) offspring are less likely to survive, due to high humidity and\/or predation, in the absence of egg-carrying females. Apparently, males did not distinguish between females according to the developmental stage of the eggs they carried. However, females were more likely to mate when carrying relatively mature versus immature eggs (14?18 days and 3?6 days post-oviposition, respectively). All egg-sacs hatched successfully when guarded by egg-carrying females because this avoided both fungal infections (at high humidity) and cannibalism by conspecific spiders. Thus, H. pluchei females may face a trade-off between mating and parental care. Further research should clarify why eggcarrying females mate and how females prevent their egg-sacs from being infected by fungi.","palabras_clave":"Maternal care|Sexual conflict|egg survival|Holocnemus pluchei"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":1405,"idioma_id":5,"titulo":"Construcción de criterios para analizar tecnologías en el marco de proyectos autogestionados","resumen":"En este artículo se toman las contribuciones teóricas de algunos autores del campo de la Filosofía de la tecnología para delimitar criterios que permitan analizar el funcionamiento de la tecnología en formas de organización social diferentes a la de la sociedad capitalista. Nos referimos a las formas en que se concibe e interactúa con la tecnología en grupos auto gestionados que se rigen por la igualdad entre sus miembros, la reflexión sobre sus prácticas, la posibilidad de construir y cuestionar sus propias normas, entre otras orientaciones. De esta manera, nos preguntamos ¿un tipo de organización social diferente requiere la puesta en juego de tecnologías diferentes? Si es así ¿qué atributos debería adquirir esa tecnología para estar acorde con esta forma de organización social?  Para responder estas preguntas, se analizará la información generada durante el trabajo de campo realizado durante un año y medio con una cooperativa auto gestionada que produce auto partes para tren delantero y suspensión.   Algunos de los resultados dan cuenta de que en esta experiencia en particular, si bien no hay un cuestionamiento y modificación estructural de la tecnología con la que están en contacto los trabajadores, se construyen criterios de valoración de la misma que son diferenciales respecto a otros contextos productivos. Además, dado el tipo de gestión colectiva que caracteriza a la auto gestión, los trabajadores tienen la posibilidad de participar en la toma de decisiones e incluso en el diseño de las tecnologías que se incorporarán al proceso productivo.","palabras_clave":"TECNOLOGÍA|AUTOGESTIÓN|AUTONOMÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":1895,"idioma_id":4,"titulo":"New analytical solutions for static two-dimensional droplets under the effects of long- and short-range molecular forces","resumen":"We report new analytical solutions for the thickness profile of partially wetting two dimensional droplets. The model includes the effects of capillarity and both short- and long-range molecular forces. We analyze the dependence of the maximum thickness, the contact angle, and the cross-sectional area on the height of the nanometric precursor film that surrounds the droplet. We found asymptotic expressions for the thickness profile and for the contact angles for large and small droplets. The results are compared to those obtained previously for polar liquids. The analytical solutions found here are useful to assess the validity of the hypothesis and the semi-analytical solutions proposed in the literature. In addition, these solutions enable the inference of information about the molecular potential from the measured steady profiles.","palabras_clave":"2D droplet   |Contact angle|Disjoining\/conjoining pressure|Partial wetting"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2446,"idioma_id":4,"titulo":"INTRINSIC KINETICS OF CLOFIBRIC ACID PHOTOCATALYTIC DEGRADATION IN A FIXED-FILM REACTOR","resumen":"This study presents a kinetic model for the photocatalytic degradation of an organic pollutant in a fixed-film reactor for water treatment. The pharmaceutical drug clofibric acid (CA) was chosen as the model compound. Experiments were carried out employing TiO2 Aeroxide P25 immobilized on the glass window of a laboratory scale reactor under UV radiation. A kinetic model to represent the degradation of CA was proposed, which explicitly takes into account the absorption of radiation by the catalytic film. Experimental results for different irradiation conditions and different number of TiO2 coatings were used to estimate intrinsic kinetic parameters. External and internal mass transfer limitations were found to be negligible under our operating conditions. Good agreement was obtained between experimental data and model predictions, with a root mean square error of 9.9%.","palabras_clave":"PHOTOCATALYSIS|INTRINSIC KINETICS|IMMOBILIZED TIO2|CLOFIBRIC ACID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2447,"idioma_id":4,"titulo":"Protein Repeats form First Principles","resumen":"Some natural proteins display recurrent structural patterns. Despite being highly similar at the tertiary structure level, repeating patterns within a single repeat protein can be extremely variable at the sequence level. We use a mathematical definition of a repetition and investigate the occurrences of these in sequences of different protein families. We found that long stretches of perfect repetitions are infrequent in individual natural proteins, even for those which are known to fold into structures of recurrent structural motifs. We found that natural repeat proteins are indeed repetitive in their families, exhibiting abundant stretches of 6 amino acids or longer that are perfect repetitions in the reference family. We provide a systematic quantification for this repetitiveness. We show that this form of repetitiveness is not exclusive of repeat proteins, but also occurs in globular domains. A by-product of this work is a fast quantification of the likelihood of a protein to belong to a family.","palabras_clave":"PROTEIN|SEQUENCE|ANALISYS|ANNOTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2448,"idioma_id":4,"titulo":"Glial alterations from early to late stages in a model of Alzheimer's disease: Evidence of autophagy involvement in A&#946; internalization","resumen":"Alzheimer´s disease (AD) is a progressive neurodegenerative disease without effective therapy. Brain amyloid deposits are classical histopathological hallmarks that generate an inflammatory reaction affecting neuronal and glial function. The identification of early cell responses and of brain areas involved could help to design new successful treatments. Hence, we studied early alterations of hippocampal glia and their progression during the neuropathology in PDAPP-J20 transgenic mice, AD model, at 3, 9, and 15 months (m) of age. At 3 m, before deposits formation, microglial Iba1+ cells from transgenic mice already exhibited signs of activation and larger soma size in the hilus, alterations appearing later on stratum radiatum. Iba1 immunohistochemistry revealed increased cell density and immunoreactive area in PDAPP mice from 9 m onward selectively in the hilus, in coincidence with prominent amyloid Congo red&#8201;+&#8201;deposition. At pre-plaque stages, GFAP+ astroglia showed density alterations while, at an advanced age, the presence of deposits was associated with important glial volume changes and apparently being intimately involved in amyloid degradation. Astrocytes around plaques were strongly labeled for LC3 until 15 m in Tg mice, suggestive of increased autophagic flux. Moreover, &#946;-Amyloid fibrils internalization by astrocytes in in vitro conditions was dependent on autophagy. Co-localization of Iba1 with ubiquitin or p62 was exclusively found in microglia contacting deposits from 9 m onward, suggesting torpid autophagy. Our work characterizes glial changes at early stages of the disease in PDAPP-J20 mice, focusing on the hilus as an especially susceptible hippocampal subfield, and provides evidence that glial autophagy could play a role in amyloid processing at advanced stages.","palabras_clave":"Hippocampus|Alzheimer's disease|Autophagy|Glia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2451,"idioma_id":4,"titulo":"Biotransformation of halogenated nucleosides by immobilized Lactobacillus animalis 2&#8242;-N-deoxyribosyltransferase","resumen":"An immobilized biocatalyst with 20-N-deoxyribosyltransferase (NDT) activity, Lactobacillus animalis NDT (LaNDT), was developed from cell free extracts. LaNDT was purified, characterized and then immobilized by ionic interaction. Different process parameters were optimized, resulting in an active derivative (2.6 U\/g) able to obtain 1.75 mg\/g of 5-fluorouracil-20-deoxyriboside, an antimetabolite known as floxuridine, used in gastrointestinal cancer treatment. Furthermore, immobilized LaNDT was satisfactorily used to obtain at short reaction times other halogenated pyrimidine and purine 20-deoxynucleosides such as 6-chloropurine-20-deoxyriboside (4.9 U\/g), 6-bromopurine-20-deoxyriboside (4.3 U\/g), 6-chloro-2-fluoropurine-20-deoxyriboside (5.4 U\/g), 5-bromo-20-deoxyuridine (2.8 U\/g) and 5-chloro-20-deoxyuridine (1.8 U\/g) compounds of pharmaceutical interest in antiviral or antitumor treatments. Besides, increasing the biocatalyst amount 8 times per volume unit allowed obtaining a 5-fold improvement in floxuridine biotransformation. The developed biocatalyst proved to be effective for the biosynthesis of a wide spectrum of nucleoside analogues by employing an economical, simple and environmentally friendly methodology.","palabras_clave":"Green chemistry|Floxuridine|DEAE-Sepharose|Antiviral agents"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2452,"idioma_id":4,"titulo":"Obstacle problems and maximal operators","resumen":"Fix two differential operators, $L_1$ and $L_2$and define a sequence of functions inductively by considering $u_1$ as the solution for the Dirichlet problem for an operator $L_1$ and then $u_n$ as the solution to the obstacle problem for an operator $L_i$ ($i=1,2$ alternating them) with obstacle given by the previous term $u_{n-1}$ in a domain $Omega$ and a fixed boundary datum $g$ on $partial Omega$. We show that in this way we obtain an increasing sequence that converge uniformly to a viscosity solution to the minimal operator associated with $L_1$ and $L_2$, that is, the limit $u$ verifies $min { L_1 u, L_2 u } =0$ in $Omega$ with $u=g$ on $partial Omega$.","palabras_clave":"Dirichlet Boundary Conditions| Obstacle Problems|Maximal Operators"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2457,"idioma_id":5,"titulo":"Ley impositiva 2015; principales modificaciones al código Fiscal de Mendoza","resumen":"Se analiza de modo integral, las modificaciones  introducidas al Código Fiscal de Mendoza, por parte de la ley impositiva 8837 (2016) tanto en lo atinente a las modificaciones al procedimiento, como a las cuestiones de fondo de cada uno de los tributos.","palabras_clave":"Derecho-impositivo|Derecho-fiscal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2459,"idioma_id":5,"titulo":"Leer en la maleza. El pasaje hacia Moro  desde  el  seno  bellatineano?","resumen":"¿Cómo se construye una lectura y escritura de a dos partiendo sólo de una línea, un verso, que despunta todo una espesura textual y poética? Este fue el punto de partida y el momento, que gracias al préstamo de Benjamin podemos llamar ?parpadeo? (2005), en que entendimos que a veces leer en términos literarios, en términos críticos, es dejar de leer en la superficie para leer en la maleza. Este es el juego que quisimos jugar para introducirnos en el pasaje que Mario Bellatin nos abre en Efecto invernadero (1995) y así penetrar en el universo de César Moro a través de un nombre, a través de ?Antonio?.","palabras_clave":"César Moro|Escrituras compuestas|Mario Bellatin|Pasajes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2578,"idioma_id":4,"titulo":"Maternal saturated fat-rich diet promotes leptin resistance in fetal liver lipid catabolism and programs lipid homeostasis impairments in the liver of rat offspring.","resumen":"We aimed to analyze if an overload of saturated fat in maternal diet induced lipid metabolic impairments in livers from rat fetuses that persist in the offspringand to identify potential mechanisms involving fetal leptin resistance.Female rats were fed either a diet enriched in 25% of saturated fat (SFD rats) or a regular diet (controls). Fetuses of 21 days of gestation and offspring of 21and 140 days of age were obtained and plasma and liver were kept for further analysis. Livers from a group of control and SFD fetuses were cultured in thepresence or absence of leptin. Leptin or vehicle was administered to control fetuses during the last days of gestation and, on day 21, fetal livers and plasma wereobtained. Lipid levels were assessed by thin-layer chromatography and mRNA gene expression of CPT1, ACO and PPAR&#945; by RT-PCR.Liver lipid levels were increased and CPT1 and ACO were down-regulated in fetuses and offspring from SFD rats compared to controls. After the culturewith leptin, control fetal livers showed increased ACO and CPT1 expression and decreased lipid levels, while fetal livers from SFD rats showed no changes.Fetal administration of leptin induced a decrease in ACO and no changes in CPT1 expression.In summary, our results suggest that a saturated fat overload in maternal diet induces fetal leptin resistance in liver lipid catabolism, which might becontributing to liver lipid alterations that are sustained in the offspring.© 2015 Elsevier Inc. All rights reserved.","palabras_clave":"leptin|liver catabolism|Saturated fat rich diet|offspring|fetus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2579,"idioma_id":4,"titulo":"Seasonal changes in organotin compounds in sediments from the Bahía Blanca Estuary","resumen":"Twenty-four surface sediment samples werecollected in the Bahía Blanca Estuary (Argentina) during2013 in order to assess the occurrence of butyltin compounds.Then, six areas ranging from zones with intensivemaritime traffic to other rural\/recreational zones at theinner utmost point of the estuary were periodically sampled(3 months) along the whole year. After extraction\/derivatizationassisted by ultrasound, three target compounds?including monobutyltin (MBT), dibutyltin (DBT) and tributyltin(TBT)?were analyzed by means of gas chromatography\/mass spectrometry (GS\/MS). Total butyltin(TBts) levels ranged from 73.5 to 395.2 ng Sn g-1 dry wtTBT was the minor contributor, accounting for 12.6 % ofthe TBts. On the other hand, MBT, for which no baselineprecedents were available, accounted for the 51.4 % ofTBts. Sampling sites could be classified, in terms ofenvironmental health regarding the organotin compounds(OTs) relationship, each from lightly (3?20 ng Sn g-1 drywt) to moderately polluted (20?100 ng Sn g-1 dry wt). Inthe light of TBts levels, distributions and covariation with environmental parameters such as granulometry and sedimentorganic carbon the occurrence of DBT and MBTcould not be uniquely attributed to the degradation pathwayof the TBT; in fact, results outlined the possible contributionof some punctual and diffuse sources at the areasuch as refineries, PVC industry inputs and the city hallmain sewage.","palabras_clave":"Organotin compounds \u0002| Ultrasound \u0002 |Sediments \u0002| Bahía Blanca Estuary"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2580,"idioma_id":4,"titulo":"Solvent effects on the photophysical properties of Bu4N[(4,4&#8242;-bpy)Re(CO)3(bpy-5,5&#8242;- diCOO)] complex. A combined experimental and computational study","resumen":"Thephotophysical properties of the complex Bu4N[(4,4´-bpy)Re(CO)3(bpy-5,5'-diCOO)]were studied in protic and aprotic media with the aid of steady-state andtime-resolved techniques and TD-DFT calculations. The absorption spectrum aswell as the steady state and time resolved luminescence of the Re(I) complex displaya marked solvent effect. The highest and lowest energy absorption bandsexperience a bathochromic shift as the polarity of the solvent decreases. Inaddition, the lowest energy band broadens. Two luminescence bands were observedaround 430 and 600 nm in protic organic solvents like alcohols. The high energyemission is observed solely in aqueous solutions, while in aprotic solvents onlythe low energy luminescence is detected. TD-DFT calculations allowed us toidentify the main electronic transitions in the low energy region as 1MLLCTRe(CO)3®4,4´-bpy and 1MLLCTRe(CO)3®bpy-5,5'-diCOO. The simulated absorption spectra of the Re(I)complex in H2O, protic (EtOH, MeOH) and aprotic (CHCl3, CH2Cl2,CH3CN) organic solvents follow the experimental absorption spectrawith reasonable accuracy both in position and relative intensities. Themagnitude of the calculated dipole moment (m) increases with the dielectric constant of thesolvent (er).Besides, the energy of 1MLLCTRe(CO)3®4,4´-bpy also increases with er.However, the energy of the 1MLLCTRe(CO)3®bpy-5,5'-diCOO transition is rather insensitive to er.This disparity is attributed to the fact that the 1MLLCTRe(CO)3®4,4´-bpy transition is nearly parallel to the orientation of m while the 1MLLCTRe(CO)3®bpy-5,5'-diCOO transition is almost perpendicular to it. UnrestrictedTD-DFT calculations were successfully applied to the triplet species. It isobserved that in the triplet state the Re-N distances are shortened while Re-Cdistances are elongated relative to the ground state. The calculated emissionenergy by TD-DFT and\/or D(SCF) methods was compared to the experimentalemission maximum in chloroform. All the experimental results as well as thetheoretical calculations indicate that solvent effects on the steady state andtime resolved luminescence of the Re(I) complex can be accounted by the coexistenceof 3MLLCTRe(CO)3®4,4´-bpy, 3MLLCTRe(CO)3®bpy-5,5'-diCOO and 1IL excited states.","palabras_clave":"Re(I) tricarbonyl complex|Solvent effects|TD-DFT|Photophysics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2581,"idioma_id":4,"titulo":"Impact of whale-watching on the short-term behavior of Southern right whales (Eubalaena australis) in Patagonia, Argentina","resumen":"In the last 30 years, whale watching tours in Patagonia, which are primarily based on viewing pods of Southernright whale, have become increasingly popular. The aim of this study was to evaluate the impact of whalewatching boat trips on the behavior of whales. Data were analyzed by means of a Generalized Linear Model using a log-link function for categorical data. The model that best fitted the data had four selected first-order interactions among factors. Whales showed short-term  eactions to boats, changing their behavior in response to the approaching boats. If the boat approached appropriately (i.e. with the engines off),whales reacted positively by approaching the boat and seeking contact, whereas if the boat approached inappropriately (i.e. with the engines on), whales reacted negatively by moving away from the boat and avoiding contact. The results of this study may have  significant implications for whale watching regulations and their enforcement.","palabras_clave":"Whale watching|Southern right whale|Short-term change|Behavioral responses|Patagonia Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2582,"idioma_id":4,"titulo":"Driving CARs into Sweet Roads: Targeting Glycosylated Antigens in Cancer","resumen":"Engineering T cells with chimeric antigen receptors (CARs) has demonstrated remarkable success in eradicating hematological malignancies. In this issue of Immunity, June and colleagues demonstrate the broad antitumor efficacy of a newly-designed CAR targeting the O-linked hypoglycosylated epitopes Tn and sialyl-Tn on cancer-associated MUC-1.(Este articulo es un preview de un articulo original: Posey, A.D., Schwab, R.D., Boesteanu, A.C., Steentoft, C., Mandel, U., Engels, B., Stone, J.D., Madsen, T.D., Schreiber, K., Haines, K.M., et al. (2016). Immunity 44, 1444?1454.)","palabras_clave":"chimeric antigen receptors (CARs)|Tn and sialyl Tn antigens|glycobiology|immunotherapy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2583,"idioma_id":4,"titulo":"Overcrowding-mediated stress alters cell proliferation in key neuroendocrine areas during larval development in Rhinella arenarum","resumen":"Exposure to adverse environmental conditions can elicit a stress response, which results in an increase in endogenous corticosterone levels. In early life stages, it has been thoroughly demonstrated that amphibian larval growth and development is altered as a consequence of chronic stress by interfering with the metamorphic process, however, the underlying mechanisms involved have only been partially disentangled. We examined the effect of intraspecific competition on corticosterone levels during larval development of the toad Rhinella arenarum and its ultimate effects on cell proliferation in particular brain areas as well as the pituitary gland. While overcrowding altered the number of proliferating cells in the pituitary gland, hypothalamus, and third ventricle of the brain, no differences were observed in areas which are less associated with neuroendocrine processes, such as the first ventricle of the brain. Apoptosis was increased inhypothalamic regions but not in the pituitary. With regards to pituitary cell populations, thyrotrophs but not somatoatrophs and corticotrophs showed a decrease in the cell number in overcrowded larvae. Our study shows that  alterations in growth and development, produced by stress, results from an imbalance in the neuroendocrine systems implicated in orchestrating the timing of metamorphosis.","palabras_clave":"stress|proliferation|pituitary|development"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2584,"idioma_id":4,"titulo":"Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon.","resumen":"Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on citrus industry. Extensive information is available about disease, nevertheless the study of plant-pathogen interactions could provide new information in the understanding of citrus canker disease., but genetic bases promoting the disease are still unclear. A new isolate has been identified, Xcc AT, which has a high genetic similarity (>90%) to virulent Xcc T reference strain based on genetic clustering analyses of the rep-PCR fingerprinting patterns, but it does not produce cankerous lesions in a host-specific interaction with Citrus limon. In this study, we compared C. limon responses to Xcc AT and to the virulent Xcc reference T strain at both histological and transcriptional levels. Histologically, leaves inoculated with Xcc AT did not exhibited neither a typical disordering of the spongy mesophyll, nor a swelling of epidermisal. A particular content (undetermined) was also found in mesophyll cells near stomata, together with an increased starch accumulation. The transcriptomic profiles were compared by using cDNA-AFLP technique. A total of 121 fragments derived from transcript (TDF) were either specifically induced or repressed by the isolates, and 62 were sequenced. Analysis of global expression identified different classes of genes known to be involved in plant?pathogen interactions. This work constitutes the first approach in the study of the specific interaction between the avirulent Xcc AT isolate and C. limon.","palabras_clave":"Gene expression|Canker|Plant defense|cDNA-AFLP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2585,"idioma_id":4,"titulo":"Light pollution: the possible consequences of excessive illumination on retina.","resumen":"Light is the visible part of the electromagnetic radiation within a range of 380-780&#8201;nm; (400-700 on primates retina). In vertebrates, the retina is adapted to capturing light photons and transmitting this information to other structures in the central nervous system. In mammals, light acts directly on the retina to fulfill two important roles: (1) the visual function through rod and cone photoreceptor cells and (2) non-image forming tasks, such as the synchronization of circadian rhythms to a 24&#8201;h solar cycle, pineal melatonin suppression and pupil light reflexes. However, the excess of illumination may cause retinal degeneration or accelerate genetic retinal diseases. In the last century human society has increased its exposure to artificial illumination, producing changes in the Light\/Dark cycle, as well as in light wavelengths and intensities. Although, the consequences of unnatural illumination or light pollution have been underestimated by modern society in its way of life, light pollution may have a strong impact on people´s health. The effects of artificial light sources could have direct consequences on retinal health. Constant exposure to different wavelengths and intensities of light promoted by light pollution may produce retinal degeneration as a consequence of photoreceptor or retinal pigment epithelium cells death. In this review we summarize the different mechanisms of retinal damage related to the light exposure, which generates light pollution.","palabras_clave":"retina|daño por luz|degeneracion retiniana|luz"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2594,"idioma_id":4,"titulo":"Fumagillin control on Nosema Ceranae (Microsporidia: Nosematidae) infection in Honey Bee (Hymenoptera: Apidae) Colonies from Argentina","resumen":"Information on the long-term consequences of Nosema.ceranae in honey bee lifespan and effectiveness of its control with fumagillin is scarce and varied. . Our objective was to evaluate the effectiveness of the antibiotic fumagillin to control N.ceranae in hives in east-central Argentina. Honey bee hives were assigned to 3 different experimental treatments, a control group with un-treated hives, a preventive strategy group with hives treated monthly, and a monitoring strategy group with hives treated according to a N. ceranae level threshold. Apiaries were monitored monthly during fall-winter 2009 and 2010 and N. ceranae spore intensity and honey bee colony strength measures were estimated. Fumagillin-treated colonies hadreduced N. ceranae spores load in2010 compared to control colonies. However, there was no significant difference between treated and control groups for colonies strength measures including adult bee population, bee brood availability, honey or pollen.Fumagillin treatment reduced N. ceranae intensities, but had little effect on colonies. The bee population during winter was reduced in treated as well as in control colonies. Our results clarify that fumagillin treatment should be at least reviewed and that further research should be conducted to get a more complete perspective of Nosemosis disease.","palabras_clave":"nosema|fumagillin|apiculture|antibiotic control"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2596,"idioma_id":4,"titulo":"Assessment of genetic and epigenetic changes in virus-free garlic (Allium sativum L.) plants obtained by meristem culture followed by in vitro micropropagation","resumen":"Garlic (Allium sativum) is a worldwide crop of economic importance susceptible to viral infections that can cause significant yield losses. Meristem tissue culture is the most employed method to sanitize elite cultivars. Often the virus-free garlic plants obtained are multiplied in vitro (micro propagation). However, it was reported that micro-propagation frequently produces somaclonal variation at the phenotypic level, which is an undesirable trait when breeders are seeking to maintain varietal stability. We employed amplification fragment length polymorphism and methylation sensitive amplified polymorphism (MSAP) methodologies to assess genetic and epigenetic modifications in two culture systems: virus-free plants obtained by meristem culture followed by in vitro multiplication and field culture. Our results suggest that garlic exhibits genetic and epigenetic polymorphism under field growing conditions. However, during in vitro culture system both kinds of polymorphisms intensify indicating that this system induces somaclonal variation. Furthermore, while genetic changes accumulated along the time of in vitro culture, epigenetic polymorphism reached the major variation at 6 months and then stabilize, being demethylation and CG methylation the principal conversions. Cloning and sequencing differentially methylated MSAP fragments allowed us to identify coding and unknown sequences of A. sativum, including sequences belonging to LTR Gypsy retrotransposons. Together, our results highlight that main changes occur in the initial 6 months of micro propagation. For the best of our knowledge, this is the first report on epigenetic assessment in garlic.","palabras_clave":"AFLP|MSAP|MSAP fragment cloning|retrotransposon|somaclonal variation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2603,"idioma_id":4,"titulo":"On basic chordal graphs and some of its subclasses","resumen":"Basic chordal graphs arose when comparing clique trees of chordal graphs and compatible trees of dually chordal graphs. They were defined as those chordal graphs whose clique trees are exactly the compatible trees of its clique graph.In this work, we consider some subclasses of basic chordal graphs. One of them is the class of hereditary basic chordal graphs, which will turn out to have many possible characterizations. Those characterizations will show that the class was already studied, but under different names and in different contexts.We also study the connection between basic chordal graphs and some subclasses ofchordal graphs with special clique trees, like DV graphs and RDV graphs. As a result, it will be possible to define the classes of basic DV graphs and basic RDV graphs.Additionally, we study the behavior of the clique operator over all the considered subclasses.","palabras_clave":"CHORDAL GRAPH|DUALLY CHORDAL GRAPH|BASIC CHORDAL GRAPH|DV GRAPH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2669,"idioma_id":4,"titulo":"Evidence for the involvement of hydraulic root or shoot adjustments as mechanisms underlying water deficit tolerance in two Sorghum bicolor genotypes","resumen":"Sorghum bicolor (L.) Moench is an ancient drought-tolerant crop with potential to sustain high yieldseven in those environments where water is limiting. Understanding the performance of this species inearly phenological stages could be a useful tool for future yield improvement programs. The aim of thiswork was to study the response of Sorghum seedlings under water deficit conditions in two genotypes(RedLandB2 and IS9530) that are currently employed in Argentina. Morphological and physiologicaltraits were studied to present an integrated analysis of the shoot and root responses. Although bothgenotypes initially developed a conserved and indistinguishable response in terms of drought toleranceparameters (growth rate, biomass reallocation, etc.), water regulation displayed different underlyingstrategies. To avoid water loss, both genotypes adjusted their plant hydraulic resistance at different levels:RedLandB2 regulated shoot resistance through stomata (isohydric strategy), while IS9530 controlled root resistance (anisohydric strategy). Moreover, only in IS9530 was root hydraulic conductance restricted in the presence of HgCl2, in agreement with water movement through cell-to-cell pathways and aquaporins activity. The different responses between genotypes suggest a distinct strategy at the seedling stage and add new information that should be considered when evaluating Sorghum phenotypic plasticity in changing environments.","palabras_clave":"relaciones hidricas|transporte de agua|raiz|estrés hídrico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2675,"idioma_id":4,"titulo":"Conformational transition of A&#946;42 inhibited by a mimetic peptide. A molecular modeling study using QM\/MM calculations and QTAIM analysis","resumen":"The main pathogenic event in Alzheimer?s disease is believed to be the aggregation of the amyloidb-peptides into toxic aggregates. In a previous work we designed a mimetic peptide possessing asignificant aggregation modulating effect by means of a molecular modeling study, using a pentamericmodel as a molecular target. Considerable experimental evidence indicates that oligomers as small asdimers have been involved in this disease. Therefore, an alternative therapeutic strategy might be toblock the oligomerization at a monomeric level. To this end, using an Ab42monomeric model, weexplored the capacity and mechanism of our mimetic peptides to stabilize thea-helical conformationwhile preventing the formation ofb-sheet structures. Long time molecular dynamics simulations andMM?GBSA analysis were coupled to investigate this issue. In addition, a combined ONIOM?QTAIManalysis was used to identify at a quantum level the most relevant interactions between Ab42and thisinhibitor. The computational analysis presented here pointed out six important residues of Ab42(Lys16, Val36, Gly37, Gly38, Val39 and Val40) that strongly interact with our mimetic peptide, providingclues about the functional groups that might be modified in order to obtain more potent inhibitors","palabras_clave":"QTAIM|ONIOM|AMYLOID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2676,"idioma_id":4,"titulo":"Phospholamban ablation rescues the enhanced propensity to arrhythmias of mice with CaMKII-constitutive phosphorylation of RyR2-S2814 site","resumen":"Background:Mice withconstitutive pseudo-phosphorylation at Ser2814-RyR2 (S2814D+\/+), increasethe propensity to arrhythmias under &#946;-adrenergic stress conditions. Althoughabnormal Ca2+ release from the SR has been linked to arrhythmogenesis, the role playedby SR Ca2+ uptakeremains controversial. We tested the hypothesis that an increase in SR Ca2+ uptake is able to rescue the increased arrhythmiapropensity of S2814D+\/+ mice. Methodsand Results: Wegenerated PLN-deficient\/S2814D+\/+ knock-in mice by crossing the twocolonies (SD+\/+\/KO mice). SD+\/+\/KOmyocytes exhibited both, increased SR Ca2+ uptake seen in PLNKO myocytesand diminished SR Ca2+ load (relative to PLNKO), characteristic ofS2814D+\/+ myocytes. Ventricular arrhythmias evoked bycatecholaminergic challenge (caffeine\/epinephrine) in S2814D+\/+ mice in vivo orprogrammed electric stimulation and high extracellular Ca2+ inS2814D+\/- hearts ex vivo, were significantly diminished by PLN ablation. At the myocyte level, PLNablation converted the arrhythmogenic Ca2+ waves evoked by high extracellular Ca2+ provocation in S2814D+\/+ mice intonon-propagated Ca2+ mini-waveson confocal microscopy. Myocyte Ca2+ waves, typical of S2814D+\/+ mice, could be evoked in SD+\/+\/KOcells by partially inhibiting SERCA2a. A mathematical human myocyte modelreplicated these results and allowed for predicting the increase in SR Ca2+ uptake required to prevent the arrhythmias inducedby a CaMKII-dependentleaky RyR2. Conclusions: Ourresults demonstrate that increasing SR Ca2+ uptake byPLN ablation can prevent the arrhythmic events triggered by SR Ca2+ leak dueto CaMKII-dependent phosphorylation of RyR2-S2814 site and underscore thebenefits of increasing SERCA2a activity on SR Ca2+ triggeredarrhythmias.","palabras_clave":"Arrhythmias|calcium|CaMKII|mathematical model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2677,"idioma_id":4,"titulo":"Conditioned breathing depression during neonatal life as a function of associating ethanol odor and the drug's intoxicating effects.","resumen":"Background: Short- and long-lasting ethanol-related alterations upon the plasticity of the respiratory system have been systematically observed in human fetuses and in animal models examining the drug´s teratogenic effects. Preclinical and clinical studies also indicate that perinates learn about the sensory attributes of ethanol and readily associate them with diverse physiological effects of the state of maternal-fetal intoxication. We have recently reported that rat neonates, exposed to ethanol during late gestation, are sensitized to the drug´s depressant effects upon respiration; an effect that is potentiated when they are also re-exposed to the smell of the drug. The present study was conducted in rat neonates during a stage in development equivalent to the 3rd human gestational trimester. The major goal was to analyze the consequences of mere ethanol odor exposure, the state of intoxication or the temporal contiguity between these factors upon neonatal breathing patterns. Methods: In Experiment, pups during postnatal days (PDs) 3, 5 and 7 either experienced an unscented chamber while sober or when intoxicated with ethanol (2.0 g\/kg) or an ethanol scented chamber under the state of sobriety or when suffering the state of intoxication. During this phase, breathing frequencies were recorded via whole body plethysmography. At PD 9, all pups were exposed to an ethanol scented chamber while sober and respiration rates were also recorded. A second test was based on ultrasound vocalizations (USVs) elicited by similar olfactory cue. The goal of experiment 2 was to analyze if sequential exposure to the drug sensitizes the organism to breathing alterations caused by the state of intoxication and to examine if this effect is related with changes in peripheral or central ethanol concentrations. Results: At PDs 3, 5 and 7 ethanol intoxication consistently resulted in breathing depression. At test (PD 9), pups that previously experienced the odor of the drug associated with the state of intoxication exhibited a significant breathing depression indicative of a conditioned response isodirectional relative to the unconditioned effects of ethanol. Gender effects were recorded in terms of USVs as a function of prior exposure to the drug. Experiment 2 revealed that sequential exposure to ethanol sensitizes the organism to the drug´s respiratory depressant effects and that this phenomenon is independent from changes in ethanol metabolism. Conclusions: These results indicate that early in ontogeny, the plasticity of the respiratory network is affected by associative learning mechanisms based on the contingency between ethanol chemosensory perception and the drug´s alterations upon breathing. Furthermore, sequential exposure to the state of intoxication sensitizes the organism to ethanol-related respiratory depressions, a phenomenon that can increase the detrimental effects of the drug upon the physiological integrity of the organism or even potentiate associative learning processes leading to early breathing disruptions.","palabras_clave":"NEONATAL ETHANOL INTOXICATION|OLFACTORY STIMULATION|SENSITIZATION|BREATHING DEPRESSION|CONDITIONED BREATHING RESPONSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2678,"idioma_id":5,"titulo":"Estabilidad de antocianinas durante el almacenamiento de jugos de arándanos","resumen":"Background: The blueberries and blueberry products has great nutritional value, primarily becauseit has high anthocyanin content. Anthocyanins are potent antioxidants and have high radical-scavengingactivities. Thus, the blueberry and blueberry products has become very appealing to consumers interestedin functional foods. However, the anthocyanins content is affected by heat treatment and subsequentstorage. The kinetics degradation of anthocyanins can be evaluated from a thermodynamic perspective,based on activation functions such as free energy, enthalpy, entropy and activation energy. Objectives:pasteurization effect and anthocyanins stability were studied during storage of pasteurized and nonpasteurizedblueberries juices. Methods: Pasteurized and non-pasteurized blueberries juices were storeat -18, 0, 5 and 10°C during 148 days. Total monomeric anthocyanins concentration was quantified atdifferent times. Principal Components Analysis was performed and experimental results were adjustedto zero and first-order kinetic models as well as to Arrhenius and Eyring ones. Results: A decrease intotal monomeric anthocyanins original concentration was 28.5 % due to pasteurization while for all temperaturesstudied, the reduction followed a first-order kinetic during storage. Degradation rate constantvaried between 0.0080 - 0.0084 days-1 and half-life, 75 - 87 days for non-pasteurized juices, whereasthese parameters were among 0.0023 - 0.0060 days-1 and 116 - 301 days, respectively for pasteurizedones. Activation energy was 44.66 kJ\/mol while Gibbs free energy, enthalpy and entropy were 83.80 kJ\/mol, 42.35 kJ\/mol and -139.09 J\/mol. K respectively, for the latter juices. Conclusions: Pasteurizationcaused in a 28.5 % loss of initial total monomeric anthocyanins. Anthocyanins stability was higher inpasteurized blueberries juices and resulted even bigger when store at 0°C, while in pasteurized juicesstored -18 ° C were less stable anthocyanins.","palabras_clave":"ANTOCIANINAS|ESTABILIDAD|CINETICA DE DEGRADACION|JUGO DE ARANDANOS|ALMACENAMIENTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2681,"idioma_id":4,"titulo":"Lift-and-project ranks of the stable set polytope of joined a-perfect graphs","resumen":"In this paper we study lift-and-project polyhedral operators dened by Lovaszand Schrijver and Balas, Ceria and Cornuejols on the clique relaxation of thestable set polytope of web graphs. We compute the disjunctive rank of allwebs and consequently of antiweb graphs. We also obtain the disjunctiverank of the antiweb constraints for which the complexity of the separationproblem is still unknown. Finally, we use our results to provide bounds ofthe disjunctive rank of larger classes of graphs as joined a-perfect graphs,where near-bipartite graphs belong.","palabras_clave":"Polyhedral combinatorics|lift-and-project operators|web graph|stable set polytope"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2761,"idioma_id":4,"titulo":"Synthesis of a 3&#8209;Thiomannoside","resumen":"An efficient and straightforward synthesis of a novel 3-thiomannoside derivative (1,2,4,6-tetra-O-acetyl-3-S-acetyl-3-thio-&#946;-d-mannopyranoside) was developed starting from levoglucosenone. A xanthate?thiocarbonate exchange under acidic conditions was the key step for the new C?S bond. The product was obtained enantiospecifically in very good overall yield.","palabras_clave":"3-THIOMANNOSIDE|LEVOGLUCOSENONE|XANTATE-THIOCARBONATE|ENANTIOSPECIFIC SYNTHESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2762,"idioma_id":4,"titulo":"A Brucella spp. Protease Inhibitor Limits Antigen Lysosomal Proteolysis, Increases Cross-Presentation, and Enhances CD8 + T Cell Responses","resumen":"In this study, we demonstrate for the first time that the outer membrane protein U-Omp19 from Brucella spp. is a competitive inhibitor of human cathepsin L. U-Omp19 inhibits lysosome cathepsins and APC derived microsomes activity in vitro and partially inhibits lysosomal cathepsin L activity within live APCs. Co-delivery of U-Omp19 with the Ag can reduce intracellular Ag digestion and increases Ag half-life in DC. U-Omp19 retains the Ag in Lamp-2 positive compartments after its internalization and promotes a sustained expression of MHCI-peptides complexes in the cell surface of DCs. Consequently, U-Omp19 enhances Ag cross-presentation by DCs to CD8+ T cells. U-Omp19 s.c. delivery induces the recruitment of CD11c+ CD8&#945;+ DCs and monocytes to lymph nodes while it partially limits in vivo Ag proteolysis inside DCs. Accordingly, this protein is able to induce CD8+ T cell responses in vivo against co-delivered Ag. Anti-tumor responses were elicited after U-Omp19 co-administration increasing mice survival in a murine melanoma challenge model. Altogether, these results indicate that a cysteine protease inhibitor from bacterial origin could be a suitable component of vaccine formulations against tumors.","palabras_clave":"BRUCELLA|PROTEASE INHIBITORS|CROSS-PRESENTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2763,"idioma_id":4,"titulo":"Feed supplementation with avian origin strains of Propionibacterium acidipropionici contributes to mucosa development in early stages of rearing broiler chickens","resumen":"Different studies in animal rearing claim the probiotic potential of species of the genus Propionibacterium. The effects of strains of Propionibacterium acidipropionici isolated from poultry intestine on, microbiota activity and intestinal mucosa development were investigated in the early stage of rearing chicks and the safety of the dose used was investigated. The strains P. acidipropionici LET105 and LET107, administered as monoculture to chicks from the 1st to 14th days of life in a daily dose of 106 CFU\/mL administered in the drinking water resulted harmless. The animals arrived at the expected weight for age and no differences were observed with respect to the food intake and water consumption related to control without bacteria administration. The analysis of microbiota composition revealed the presence of propionibacteria at the middle and end of the trial only in treated groups. Normal development of lactic acid bacteria and bifidobacteria, and slow colonization by Bacteroides at the 7th day of the study was observed in the same groups. Analysis of the organic acids concentrations in the cecal content of birds revealed higher lactic acid and lower butyric acid production. Lower short chain fatty acids total concentration than expected during treatment was related to a better development of the gut mucosa. Increase in length of villus-crypt units, goblet cells counts and neutral mucins production were evidenced. Higher mucus secretion produced by dietary supplementation with propionibacteria could provide increased protection against pathogens.","palabras_clave":"DAIRY PROPIONIBACTERIA|CHICKEN|PROBIOTIC|MICROBIOTA|GUT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2799,"idioma_id":4,"titulo":"Enhanced antimicrobial activity of a peptide derived from human lysozyme by arylation of its tryptophan residues","resumen":"Antimicrobial peptides are valuable agents to fight antibiotic resistance. These amphipatic species display positively charged and hydrophobic amino acids. Here, we enhance the local hydrophobicity of a model peptide derived from human lysozyme (107RKWVWWRNR115) by arylation of its tryptophan (Trp) residues, which renders a positive effect on Staphylococcus aureus and Staphylococcus epidermidis growth inhibition. This site-selective modification was accessed by solid-phase peptide synthesis using the non-proteinogenic amino acid 2-aryltryptophan, generated by direct C-H activation from protected Trp. The modification brought about a relevant increase in growth inhibition: S. aureus was fully inhibited by arylation of Trp 112 and by only 10% by arylation of Trp 109 or 111, respect to the non-arylated peptide. On the other hand, S. epidermidis was fully inhibited by the three arylated peptides and the parent peptide. The minimum inhibitory concentration was significantly reduced for S. aureus depending on the arylation site.","palabras_clave":"ANTIMICROBIAL|PEPTIDE|ENHANCED|LYSOZYME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2844,"idioma_id":5,"titulo":"Programar con Scratch en contextos educativos:  ¿Asimilar directrices o co-construir Tecnologías para la Inclusión Social?","resumen":"Actualmente se observa un interés por introducir nociones de programación en contextos educativos a partir de diferentes herramientas, dentro de las cuales se destaca Scratch. El presente artículo indaga si los discursos y directrices de sus desarrolladores para la enseñanza y aprendizaje de la programación, pueden promover Prácticas Educativas Mediatizadas que viabilicen la co-construcción de Tecnologías para la Inclusión Social en contextos formales y no formales. El estudio se basa en los fundamentos teóricos del enfoque socio-técnico y el marco teórico-metodológico del Dispositivo Hipermedial Dinámico, aplicando una metodología cualitativa y exploratoria. A partir del análisis de resultados, se concluye que las barreras para la co-construcción de Tecnologías para la Inclusión Social en el contexto educativo no se relacionan con limitaciones de la herramienta en sí, sino con la orientación instrumental de las prácticas de enseñanza y aprendizaje que se han puesto en obra.","palabras_clave":"PROGRAMACION COMPUTACIONAL|PRACTICAS EDUCATIVAS MEDIATIZADAS|ENFOQUE SOCIO-TECNICO|DISPOSITIVO HIPERMEDIAL DINAMICO|SCRATCH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2855,"idioma_id":4,"titulo":"Modifications in nitric oxide and superoxide anion metabolism induced by fructose overload in rat  heart are prevented by (&#8722;)-epicatechin","resumen":"Fructose overload promotes functional and metabolic derangements in humans and in animal experimental models. Evidence suggests that dietary flavonoids have the ability to prevent\/attenuate the development of metabolic diseases. In this work we investigated the effects of (-)-epicatechin on the modifications induced by fructose overload in the rat heart in terms of nitric oxide and superoxide metabolism. Male Sprague Dawley rats received 10% (w\/v) fructose in the drinking water for 8 weeks, with or without (-)-epicatechin (20 mg per kg body weight per day) in the rat chow diet. These conditions of fructose overload did not lead to overt manifestations of heart hypertrophy or tissue remodeling. However, biochemical and molecular changes were observed and could represent the onset of functional alterations. (-)-Epicatechin prevented a compromised NO bioavailability and the development of oxidative stress produced by fructose overload essentially acting on superoxide anion metabolism. In this line, the increase in superoxide anion production, the overexpression of NOX2 subunit p47phox and of NOX4, the decrease in superoxide dismutase activity, and the higher oxidized\/reduced glutathione ratio installed by fructose overload were absent in the rats receiving (-)-epicatechin. These results support the hypothesis that diets rich in (-)-epicatechin could prevent the onset and progression of heart dysfunctions associated with metabolic alterations.","palabras_clave":"ANTIOXIDANTS|FLAVONOIDS|HYPERTENSION|OBESITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2856,"idioma_id":4,"titulo":"HETEROGENEOUS DISTRIBUTION OF TRACE FOSSILS ACROSS INITIAL TRANSGRESSIVE DEPOSITS IN RIFT BASINS: AN EXAMPLE FROM THE SPRINGHILL FORMATION, AUSTRAL BASIN, PATAGONIA ARGENTINA","resumen":"In the Lago San Martín region (Santa Cruz Province, Argentina), the Springhill Formation (50 to 100 m thick) overlies the syn-rift El Quemado Complex, showing outcrops with wedge-geometry. Four sedimentological logs were obtained from the field and three depositional palaeoenvironments were interpreted. The Springhill Formation starts with fluvial deposits, characterized by channels and floodplains with palaeosol development, passing transitionally to a coastal plain and, finally, to marine sedimentation. The initial sandy transgressive deposits (Facies Association 9) are the main focus of this paper, in which ten ichnogenera (Arenicolites, Bergaueria, Cylindrichnus, Diplocraterion, Macaronichnus, Palaeophycus, Planolites, Ophiomorpha, Rosselia and Skolithos) are described. Two ichnoassociations (foreshore and shoreface) were defined and their distribution was controlled by the local palaeoenvironmental conditions, mainly energy, bathymetry and grain-size of sediments. A highly bioturbated surface (BI= 4) with a limited occurrence that disappears over short distances perpendicularly to the palaeoshoreline was recognized in Section 1. This surface shows a sharp sub-horizontal gently undulating top contact with a bioturbation thickness between 15 and 25cm. This type of surface has limited usefulness as a key correlative surface, because it is spatially restricted in rift basins due to the tectonic activity, which creates high accommodation space rates. A more accurate characterization of the initial transgressive deposits of the Springhill Formation?which is the most important reservoir in southern Patagonia?could provide new ideas to solve problems in subsurface studies.","palabras_clave":"ICHNOLOGY|SPRINGHILL FORMATION|RIFT BASIN|CRETACEOUS|AUSTRAL BASIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2860,"idioma_id":4,"titulo":"Axotomy of tributaries of the pelvic and pudendal nerves induces changes in the neurochemistry of mouse dorsal root ganglion neurons and the spinal cord","resumen":"Using immunohistochemical techniques, wecharacterized changes in the expression of several neurochemicalmarkers in lumbar 4-sacral 2 (L4?S2) dorsal rootganglion (DRG) neuron profiles (NPs) and the spinal cordof BALB\/c mice after axotomy of the L6 and S1 spinalnerves, major tributaries of the pelvic (targeting pelvicvisceral organs) and pudendal (targeting perineum andgenitalia) nerves. Sham animals were included. Expressionof cyclic AMP-dependent transcription factor 3 (ATF3),calcitonin gene-related peptide (CGRP), transient receptorpotential cation channel subfamily V, member 1 (TRPV1),tyrosine hydroxylase (TH) and vesicular glutamate transporters(VGLUT) types 1 and -2 was analysed seven days after injury. L6?S1 axotomy induced dramatic de novoexpression of ATF3 in many L6?S1 DRG NPs, and parallelsignificant downregulations in the percentage of CGRP-,TRPV1-, TH- and VGLUT2-immunoreactive (IR) DRGNPs, as compared to their expression in uninjured DRGs(contralateral L6?S1-AXO; sham mice); VGLUT1 expressionremained unaltered. Sham L6?S1 DRGs onlyshowed a small ipsilateral increase in ATF3-IR NPs (othermarkers were unchanged). L6?S1-AXO induced de novoexpression of ATF3 in several lumbosacral spinal cordmotoneurons and parasympathetic preganglionic neurons;in sham mice the effect was limited to a few motoneurons.Finally, a moderate decrease in CGRP- and TRPV1-likeimmunoreactivitieswas observed in the ipsilateral superficialdorsal horn neuropil. In conclusion, injury of a mixedvisceral\/non-visceral nerve leads to considerable neurochemicalalterations in DRGs matched, to some extent, inthe spinal cord. Changes in these and potentially othernociception-related molecules could contribute to pain dueto injury of nerves in the abdominopelvic cavity.","palabras_clave":"CGRP|Glutamate|Nerve Injury|TRPV1|Visceral Pain"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2903,"idioma_id":4,"titulo":"AtPDCD5 Plays a Role during dark-senescence in Arabidopsis","resumen":"In this work, we investigated the role of an Arabidopsis protein, AtPDCD5, duringsenescence after a 24h-dark period. Previously, we demonstrated that AtPDCD5participates in programmed cell death (PCD) after UV-B exposure and in ageinducedsenescence. The results presented here, together with previous data,demonstrate that AtPDCD5 not only plays an important role during DNA damageresponses induced by UV-B radiation, but also takes part in PCD programs suchas dark-induced senescence in Arabidopsis.","palabras_clave":"muerte celular programada|arabidopsis|senescencia|PDCD5"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2945,"idioma_id":4,"titulo":"Oxidative stress and altered steroidogenesis in the ovary by cholinergic stimulation of coeliac ganglion in the first proestrous in rats. Implication of nitric oxide","resumen":"An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CGeSONeO) system from virgin rats in the&#64257;rst proestrous was used to test whether cholinergic stimulation of CG affects oxidative status andsteroidogenesis in the ovary. The CG and the O were placed in separate buffered-compartments, connectedby the SON, and the CG was stimulated by acetylcholine (Ach). To test a possible role of nitricoxide (NO) in the ovarian response to cholinergic stimulation of CG, aminoguanidine (AG) e an inhibitorof inducible-NO synthase was added to the O compartment. After 180 min incubation, the oxidativestatus was assessed in O whereas nitrite and steroidogenesis were assessed at 30, 120 and 180 min. Achin CG decreased the total antioxidant capacity, but increased NO production and protein carbonization inO. Ach stimulation of CG increased estradiol, but decreased progesterone release in O by reducing themRNAs related to their synthesis and degradation. The addition of AG to the O compartment caused anopposite effect, which was more pronounced in the presence of Ach in the CG compartment than in itsabsence. These results show that the stimulation of the extrinsic-cholinergic innervation of the O increasesthe concentration of NO, causes oxidative stress and modulates steroidogenesis in the &#64257;rst ratproestrous.","palabras_clave":"FIRST PROESTROUS IN RATS|ACETYLCHOLINE|AMINOGUANIDINE|OXIDATIVE STRESS|NITRIC OXIDE|OVARIAN STEROIDOGENESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2946,"idioma_id":4,"titulo":"A Bio-inspired Scheduler for Minimizing Makespan and Flowtime of Computational Mechanics Applications on Federated Clouds [Thomson ISI, IF JCR2014=1.812]","resumen":"Computational Mechanics (CM) concerns the use of computational methods to study phenomena under the principles of mechanics. A representative CM application is parameter sweep experiments (PSEs), which involves the execution of many CPU-intensive jobs and thus computing environments such as Clouds must be used.We focus on federated Clouds, where PSEs are processed via virtual machines (VM) that are lauched in hosts belonging to different datacenters, minimizing both the makespan and flowtime. Scheduling is performed at three levels: a) broker, where datacenters are selected based on their network latencies via three policies, b) infrastructure, where two bio-inspired schedulers based on Ant Colony Optimization (ACO) and Particle Swarm Optimization (PSO) for VM-host mapping in a datacenter are implemented, and c)VM, where jobs are assigned into the preallocated VMs based on job priorities. Simulated experiments performed with job data from two real PSEs show that our scheduling approach allows for a more agile job handling while reducing PSE makespan and flowtime.","palabras_clave":"CLOUD COMPUTING|COMPUTATIONAL MECHANICS|SCHEDULING|ANT COLONY OPTIMIZATION|PARTICLE SWARM OPTIMIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2949,"idioma_id":5,"titulo":"¿Qué ves cuando me ves? El puma (Puma concolor) y su representación en los medios de prensa de Patagonia Argentina","resumen":"El puma (Puma concolor) es el carnívoro de mayor tamaño de Patagonia y su persecución en la región es una constante. Es considerado por los productores ganaderos como uno de los principales predadores de ganado y causante de una gran pérdida económica. El conflicto se ha agravado en los últimos años y el señalamiento del puma como perjudicial, plaga o especie dañina tiene gran difusión. Los medios de comunicación pueden influir en la percepción que las personas tienen sobre el puma y contribuir a consolidar determinadas  percepciones sociales de la especie. A su vez, pueden avalar o legitimar las prácticas ya establecidas, que atentan contra su conservación. El objetivo de este trabajo fue identificar una de las maneras en que los medios de prensa escrita pueden construir una imagen del puma en Patagonia. El estudio se realizó a partir de los lineamientos generales del análisis ideológico del discurso de Teun Van Dijk. El corpus informativo estuvo compuesto por 68 notas periodísticas publicadas sobre la especie en 11 diarios provinciales de Patagonia, entre los años 2000 y 2013. En éstos se reconocieron los 4 mecanismos propuestos por Van  Dijk, mediante los cuales se construye la figura de un ?Nosotros? (el hombre en este caso) y la de un ?Ellos? (el puma), y se describe positivamente al primer grupo y negativamente al segundo.","palabras_clave":"puma|medios de prensa|conflicto humano-carnívoro|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":2957,"idioma_id":4,"titulo":"Highly selective Cu-modified Ni\/SiO2-Al2O3 catalysts for the conversion of maleic anhydride to gamma butyrolactone in gas phase.","resumen":"The gas phase hydrogenation of maleic anhydride (MA)to succinic anhydride (SA) and the subsequent hydrogenolysis to gamma-butyrolactone(GBL) was studied on SiO2-Al2O3 supported Nicatalysts modified with Cu and prepared by incipient wetness impregnation(Ni-I, CuNi-I) and coprecipitation-deposition (CuNi-PD) methods. The samples were characterized by N2 adsorption at -196 ºC, X-ray diffraction,temperature-programmed reduction, transmission electron microscopy and H2 chemisorption. Catalytic tests were performed between 170 and 220 ºC atatmospheric pressure in a fixed bed reactor. Crystalline NiO along with a Ni2+ phase strongly interacting with the support was observed in the oxideprecursors. The extent of the strongly interacting Ni2+ phase diminishes according to the following pattern: CuNi-PD > CuNi-I > Ni-I,along with a proportional rise of the NiO phase. The proportion of small to large metal particles, formed after reduction, followed the same pattern asthat observed for the extent of Ni2+ phase. All catalysts were veryactive in the MA hydrogenation to SA, but displayed distinct performances withrespect to the subsequent SA hydrogenolysis. Hydrogenolytic activity and GBL yield increased following the same pattern as that obtained for the extent of Ni2+ phase. In addition, the effect of raising temperature on hydrogenolytic activity was more important in the case of Ni-I and CuNi-I than for CuNi-PD. Furthermore, CuNi-PD was more selective to GBL than CuNi-I. These results showed that there is an important effect of Cu addition and preparation method on both the structure and catalytic performance of the metal Ni phase.","palabras_clave":"SELECTIVE HYDROGENATION|MALEIC ANHYDRIDE|GAMMA-BUTYROLACTONE|CU-NI CATALYSTS|PRECIPITATION-DEPOSITION METHOD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3038,"idioma_id":4,"titulo":"Structure of force networks in tapped particulate systems of disks and pentagons. II. Persistence analysis","resumen":"The force network of a granular assembly, defined by the contact network and the corresponding contact forces, carries valuable information about the state of the packing. Simple analysis of these networks based on the distribution of force strengths is rather insensitive to the changes in preparation protocols or to the types of particles. In this and the companion paper [Kondic et al., Phys. Rev. E 93, 062903 (2016)], we consider two-dimensional simulations of tapped systems built from frictional disks and pentagons, and study the structure of the force networks of granular packings by considering network´s topology as force thresholds are varied. We show that the number of clusters and loops observed in the force networks as a function of the force threshold are markedly different for disks and pentagons if the tangential contact forces are considered, whereas they are surprisingly similar for the network defined by the normal forces. In particular, the results indicate that, overall, the force network is more heterogeneous for disks than for pentagons. Such differences in network properties are expected to lead to different macroscale response of the considered systems, despite the fact that averaged measures (such as force probability density function) do not show any obvious differences. Additionally, we show that the states obtained by tapping with different intensities that display similar packing fraction are difficult to distinguish based on simple topological invariants.","palabras_clave":"Force Networks"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3070,"idioma_id":4,"titulo":"Floral development and anatomy of pistillate flowers of Lophophytum (Balanophoraceae), with special reference to the embryo sac inversion","resumen":"The extreme reduction of flowers in the Balanophoraceae has generated few and incomplete studies with scattered and often contradictory results with respect to embryology. Using light and scanning microscopy, gynoecium, ovule and embryo-sac development are described in two (of 4) species of Lophophytum. The pistillate flower lacks perianth, and it is reduced to one pistil formed by a superior, biloculate ovary and two styles with capitated stigmas. Two ategmic hemianatropous ovules are inserted on the upper portion of a central placental column. The two locules are almost completely obstructed by the ovules. The term micropyle is not applicable in its usual sense, due to the absence of integuments. The term 'micropylar pole' instead of micropyle was used to designate the apex of the nucellus, where the megaspore mother cell develops. Vascular supply is missing in the placenta and the ovule, therefore, the chalaza and the funiculus cannot be defined. The point of attachment of the nucellus to the placenta was designated 'chalazal pole'. The embryo-sac is Adoxa type. During the migration of the two pairs of nuclei to the opposite poles, the four-nucleate embryo-sac tends to take a 'J' shape. Each pair of nuclei undergoes a mitotic division to form an eight-nucleate embryo-sac. In the upper, chalazal pole of the embryo-sac, a typical egg-apparatus with a central egg-cell and two adjacent synergids cells is developed, while in the micropylar pole three antipodes are formed, all that determines an embryo-sac of reversed position. The analysis of the anatomy and development of pistillate flowers and the study of the functional architecture of ovules, carpels and embryo-sac, provide embryological data of great importance to complement phylogenetic studies in the family Balanophoraceae, and even in order Santalales.","palabras_clave":"Balanophoraceae|Embryo-sac|Holoparasite|Ovule"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3134,"idioma_id":4,"titulo":"Hydrogen cycling properties of xMg-Fe materials (x: 2, 3 and 15) produced by reactive ball milling","resumen":"This work deals with the hydrogen cycling properties of xMg-Fe (x: 2, 3 and 15) elemental powder mixtures produced via low energy ball milling under hydrogen atmosphere at room temperature. The cycling process is carried out at constant temperature of 375 °C in a closed loop device. The evolution of both hydrogen storage capacity and absorption kinetic behavior are monitored during pressure cycling. Changes induced by cycling are characterized via scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and particle size distribution (PSD). All studied materials lost part of their hydrogen storage capacity during hydrogen cycling. This capacity reduction results from a combination of two effects: Mg evaporation and the formation of Mg2FeH6 which is stable at the experimental conditions. While the hydrogenation rates are not affected, the dehydrogenation kinetic properties of the materials are influenced by the cycling process.","palabras_clave":"HYDROGEN STORAGE|CYCLING STABILITY|COMPLEX HYDRIDE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3135,"idioma_id":4,"titulo":"Effects of silica gel on reduction in gluten during several beer brewing stages","resumen":"Beer is an alcoholic beverage made with barley as a basic raw material, and therefore it is not suitable for people with celiac disease. During the brewing process, the elimination of the haze active precursor is performed at the stabilization stage. Some breweries use silica gel (SG) as stabilization precursor. This work presents the studies conducted to identify the stage where the addition of SG is more convenient in order to reduce gluten, and the effect on the yeast nutritional value due its incorporation. The incorporation of SG during fermentation allows the reduction of more gluten than the others brewery stages studied,  without changing significantly the yeast nutritional value of beer. Furthermore, the use of SG promotes the reduction of polysaccharides which obstruct filtration.","palabras_clave":"Barley|beverages|gluten|proteins."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3136,"idioma_id":4,"titulo":"Synergistic roles of climate warming and human occupation in Patagonian megafaunal extinctions during the Last Deglaciation","resumen":"The causes of Late Pleistocene megafaunal extinctions (60,000 to 11,650 years ago, hereafter 60 to 11.65 ka) remaincontentious, with major phases coinciding with both human arrival and climate change around the world. TheAmericas provide a unique opportunity to disentangle these factors as human colonization took place over anarrow timeframe (~15 to 14.6 ka) but during contrasting temperature trends across each continent. Unfortunately,limited data sets in South America have so far precluded detailed comparison. We analyze genetic and radiocarbondata from 89 and 71 Patagonian megafaunal bones, respectively, more than doubling the high-quality Pleistocenemegafaunal radiocarbon data sets from the region.Weidentify anarrowmegafaunal extinction phase 12,280 ± 110 yearsago, some 1 to 3 thousand years after initial human presence in the area. Although humans arrived immediatelyprior to a cold phase, the Antarctic Cold Reversal stadial, megafaunal extinctions did not occur until the stadialfinished and the subsequent warming phase commenced some 1 to 3 thousand years later. The increased resolutionprovided by the Patagonianmaterial reveals that the sequence of climate andextinctionevents inNorthandSouthAmerica were temporally inverted, but in both cases, megafaunal extinctions did not occur until human presence andclimate warming coincided. Overall, metapopulation processes involving subpopulation connectivity on a continentalscale appear to have been critical for megafaunal species survival of both climate change and human impacts.","palabras_clave":"Megafaunal Extinctions|Patagonia|Late Pleistocene |Climate Warming"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3237,"idioma_id":4,"titulo":"New host and distribution expansion for Pterygodermatites (Multipectines) affinis","resumen":"The present study reports the first case of infection by Pterygodermatites affinis in Lycalopex gymnocercus, and the first report of this nematode in Argentina. Examining 80 foxes from Buenos Aires province, specimens of this species were recovered from 21.25% of the small intestines and eggs were found in 7.5% of the fecal samples.","palabras_clave":"ARGENTINA|SOUTH AMERICA|WILD MAMMALS|PTERYGODERMATITES AFFINIS|PAMPAS FOX|LYCALOPEX GYMNOCERCUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3251,"idioma_id":4,"titulo":"Pharmacological activities of a propylthiouracil compound structurally modified by coordination with copper(II)","resumen":"For the first time, pharmacological activities for propylthiouracil (actually used as antithyroid drug) were determined. In addition, a new propylthiouracil copper(II) complex ([Cu(PTU)2]2) was synthesized and characterized by FTIR, EPR, UV?visible, and diffuse reflectance spectroscopies including elemental analysis, dissolution profiles, and stability studies. Taking into account the correlation between Graves? disease and the formation of reactive oxygen species (ROS) and other free radicals, the ligand and the complex were tested for their antioxidant effects on O2·&#8722; and OH· radicals. A significant increase in the disruption of OH· radical was observed for PTU and its copper(II) complex, but neither of them have the ability to dismutate the O2·&#8722; radical. Antimicrobial activities were also determined observing that the complex is very active against Gram-positive bacteria. In addition, the ability of PTU and its complex to inhibit acid and alkaline phosphatases were analyzed. Results showed that PTU had no effect, while the complex behaved as a potent ALP (alkaline phosphatases) inhibitor. Finally, albumin interaction experiments denoted high affinity towards the complex in contrast with PTU with a constant binding value two hundred times higher than the ligand and bearing two binding sites. Based on this study, it has been hypothesized that ([Cu(PTU)2]2 would be a promising candidate for further evaluation as an antioxidant, antimicrobial and phosphatase alkaline inhibitor agent.","palabras_clave":"Antioxidants|BSA interactions|Copper(II); |Propylthiouracil.|Antimicrobial activity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3252,"idioma_id":4,"titulo":"Development of the germinal epithelium and early folliculogenesis during the ovarian morphogenesis in the cichlid fish Cichlasoma dimerus (Teleostei, Perciformes)","resumen":"Formation of the germinal epithelium and folliculogenesis during ovarian development in  22 Cichlasoma dimerus were described at the light- and electron-microscopic levels. Prior to  23 gonadal differentiation, germ cells and enveloping support cells reside within an  24 inpocketing of the coelomic epithelium. Separation of the germinal and interstitial  25 compartments of the gonad by a basement membrane is apparent from early gonadal  26 development. Upon ovarian differentiation, oogonia undergo cyst-forming divisions leading  27 to the formation of clusters of interconnected cystocytes that synchronously enter meiosis,  28 becoming oocytes. At the pachytene step, each oocyte becomes individualized by  29 cytoplasmic extensions of prefollicle cells, thereby developing as an ovarian follicle.  30 Subsequent somatic reorganization leads to the formation of the ovarian lumen in a  31 cephalo-caudal gradient. As a result, the germinal epithelium becomes internalized and  32 lines the ovarian lumen. As defined by its origin from the germinal epithelium, the ovarian  33 follicle is composed of an oocyte and the surrounding follicle cells. Thecal cells derived  34 from the stroma encompass the basement membrane outside the follicle, thus forming a  35 follicle complex. A common basement membrane is shared by the germinal epithelium and  36 the follicle complex along a small portion of its surface. This point of attachment represents  37 the site at which the oocyte would be released to the ovarian lumen during ovulation.","palabras_clave":"germinal epithelium|early folliculogenesis |ovarian morphogenesis | cichlid fish |Cichlasoma dimerus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3309,"idioma_id":4,"titulo":"Prediction of volumetric data in supercritical reactors","resumen":"Pressure intensified processes are characterized by handling mixtures whose density is highly dependent on the operating conditions. In addition, fluid behavior in this type of equipment is clearly non-ideal. Therefore, the frequent assumption of ideal mixing for calculating the mixture density is inaccurate. Prediction of volumetric information of mixtures is important for the design of continuous supercritical reactors and other process equipments such as heat exchangers, high-pressure pumps, compressors, etc. The isochoric method is a simple and reliable procedure to measure mixture densities and find conditions of homogeneous operation of supercritical chemical reactor at high temperature and pressure. Moreover, it also provides PvT properties simultaneously.It is well known that the two-parameter equations of state (2P-EoS), like Soave-Redlich-Kwong or Peng-Robinson, have limitations to describe accurately the PvT properties of different fluids together with the phase transitions. The three-parameter RK-PR equation of state (3P-EoS), known as the generalized Redlich-Kwong-Peng-Robinson is able to correlate and predict volumetric properties of size-asymmetric mixtures. In this work, RK-PR, coupled with the standard quadratic mixing rules, is used to model volumetric experimental data of non-reacting size-asymmetric binary systems. Moreover, based on that modeling, we predict volumetric properties of reacting mixtures in the context of supercritical biodiesel production.","palabras_clave":"BIODIESEL PRODUCTION|PR EQUATION OF STATE|SUPERCRITICAL REACTORS|VOLUMETRIC PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3315,"idioma_id":4,"titulo":"Protocol for multivariate homogeneous zone delineation in precision agriculture","resumen":"Uniform management of agricultural fields has been increasingly replaced with environmentally based management, which is benefited by the identification of homogeneous zones within crop fields. Such zones are based on the classification of field sites into groups of homogeneous features. Multiple causative agents of variability and the response of agricultural crops should be considered for zoning. Several correlated variables are usually measured and georeferenced for this purpose at multiple sites within the field. This paper presents an approach to promoting the integration of different statistical tools for identifying homogeneous zones based on site covariates. The methodological innovation of this work involves cleaning and re-scaling of spatial data, as well as multivariate and geostatistical analyses in a logical sequence (protocol). Statistical topics for further improvement and protocol applications are noted. The analytical process has been illustrated using a rain-fed wheat crop (60 ha) from the Argentine Pampas, with apparent electrical conductivity, elevation and soil depth as master variables for zoning, and yield, soil organic matter and clay to validate the created management zones; however, it may be applied to other production systems using georeferenced data. The R scripts and the sample file to run the proposed protocol are provided as electronic supplementary material.","palabras_clave":"Spatial data |Software specifications|Spatial autocorrelation| Multivariate classification| Site-specific management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3330,"idioma_id":4,"titulo":"LOGISTICAL MOBILITY IN PLATEAUS IN CENTRAL-WESTERN SANTA CRUZ, ARGENTINA. AN APROACH FROM TECHNOLOGICAL, ARCHAEOFAUNAL AND ANTHRACOLOGICAL EVIDENCE","resumen":"The aim of this paper is to discuss hunteregatherers´ logistical mobility strategies in Central-Western Santa Cruz province during the last 2500 years BP, in a context of human adaptations to semi-desert conditions. The approach to the archaeological record is focused on integrating three independent lines of evidence: technology, zooarchaeological and anthracological analysis. Logistical mobility will be addressed focusing on two main aspects: resources transportation and variability of archaeological contexts. The observed variability has allowed shedding light on the use of the category ?logistical? used to describe plateaus. Additionally, these areas show a well-planned and scheduled use during a regionalprocess of desiccation.","palabras_clave":"LOGISTIC MOBILITY|HUNTER-GATHERERS|PATAGONIA|LATE HOLOCENE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3333,"idioma_id":4,"titulo":"Biospectroscopy, biospectrometry and imaging of Ilex paraguariensis. Basis for non-destructive quality evaluation using artificial vision","resumen":"Yerba mate (YM) is massively produced and consumed as an infusion in South America and is gainingpopularity all over the world. This product is obtained from the dried leaves of Ilex paraguariensis SaintHilaire, mixed with fragments of its dried branches. For its commercialization YM must have a minimumpercentage of leaves according to a standard classification. Until now, composition quantification hasbeen mechanically performed, thus development of new methods is still pending. In this work a quantificationmethod using solid-phase molecular fluorescence, alternately diffuse reflectance spectroscopyand an imaging technique using a scanner have been proposed. Strong differences between the spectroscopicproperties of leaves and sticks were observed and in all the cases linear correlations between theprocessed signals and composition were observed. Interesting differences in chemical composition of YMleaves and sticks were additionally obtained in this work by means of total phenol content quantification,ultraviolet matrix assisted laser-desorption ionization mass spectrometry and ultraviolet laser-desorptionionization mass spectrometry.","palabras_clave":"YERBA MATE|MALDI|BIOSPECTROSCOPY|FLUORESCENCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3354,"idioma_id":4,"titulo":"Direct field method for root biomass quantification in agroecosystems","resumen":"The present article describes a field auger sampling method for row-crop root measurements. In agroecosystems where crops are planted in a specific design (row crops), sampling procedures for root biomass quantification need to consider the spatial variability of the root system. This article explains in detail how to sample and calculate root biomass considering the sampling position in the field and the differential weight of the root biomass in the inter-row compared to the crop row when expressing data per area unit. This method is highly reproducible in the field and requires no expensive equipment and\/or special skills. It proposes to use a narrow auger thus reducing field labor with less destructive sampling, and decreases laboratory time because samples are smaller. The small sample size also facilitates the washing and root separation with tweezers. This method is suitable for either winter- or summer crop roots.","palabras_clave":"Direct method|auger sampling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3424,"idioma_id":5,"titulo":"Las Tecnologías y la enseñanza en la educación superior. USINA aplicada a la integración de conceptos enseñados en cursos de posgrado","resumen":"El objetivo de este trabajo fue implementar tecnologías de la información y la comunicación(TICs) a nivel de posgrado. En este caso particular se usó USINA, un simulador para la toma de decisionesdiseñadopor el Centro de Innovaciones en Tecnología y Pedagogía (CITEP), de la Universidad de Buenos Aires. Ésta permite trabajar con narraciones, estudio de casos y utilizar el ?error? como método pedagógico.Métodos: 1) Diseñar una secuencia de pantallas para ser montadas en USINA para utilizarla como herramienta de integración de conceptos en dos cursos de posgrado: Downstream Processing de proteínas (DSP) y Aplicaciones, síntesis y análisis de péptidos sintéticos (ASAP). 2) Realizar una encuesta anónima para evaluar la eficiencia de USINA. 3) Clase presencial para intercambiar opiniones sobre la experiencia.Resultados: En ambos cursos (DSP y ASAP) la experiencia resultó exitosa.Los alumnos consideraron que el uso de USINA permitió el andamiaje de conocimientos y la integración de conceptos al situarlos en el rol de expertos. Discusión:El uso de las TICs en los niveles educativos superiores sigue siendo una asignatura pendiente. A través de la experiencia aquí analizada se abre la posibilidad de llevar esta herramienta a otros cursos de pregrado y con mayor número de estudiantes.","palabras_clave":"Simuladores|Educación en Biotecnología|Química Orgánica|Péptidos Sintéticos|TIC|Plataforma Virtual"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3425,"idioma_id":5,"titulo":"Parámetros agronómicos y ambientales de fósforo en suelos molisoles con diferentes usos en la provincia de Buenos Aires, Argentina","resumen":"El cambio dinámico en el suelo debido a diferentes usos puede monitorearse mediante el seguimiento de variablessensibles. Para el contenido de fósforo (P) no existe un consenso acerca de la variable de medición más adecuada.En este trabajo se evaluó la utilidad de técnicas que miden disponibilidad, pérdida y capacidad de retención de P(P-Bray1, P-CaCl2, P75, ISP y GSP) para diferenciar cambios en el contenido de P del suelo, relacionados al uso dela tierra, así como la relación entre dichas técnicas. Se analizaron dos profundidades de suelo en dos sitios delsudeste de la provincia de Buenos Aires con diferentes secuencias: agrícolas (A), pastizales nativos (PN), sistemasforestales (FO) y pastizales secundarios (PS). Todas las técnicas pudieron diferenciar entre sistemas de manejo;con diferencias variables según la profundidad del suelo. Se detectaron correlaciones estrechas entre las técnicas,resultando dependientes del uso del suelo, P-Bray1 y P-CaCl2 (R2 = 0.89), P75 e ISP (R2 = 0,79) y GSPP75 o GSPISPcon P-CaCl2 (R2 = 0.82 y 0.83 respectivamente). Los sistemas A incrementaron la disponibilidad de P (P-Bray1),disminuyeron la capacidad de retención (P75 e ISP) y generaron mayor riesgo de pérdidas de P (P-CaCl2). El FOy la PS presentaron mejor disponibilidad de P respecto de PN, con mayor capacidad de retención y con menoresriesgos de pérdidas por escurrimiento. En base a los resultados, podría estimarse una forma de P en función dela determinación analítica de otra, pero de forma diferencial según el uso del suelo.","palabras_clave":"GSP|ISP|P?Bray1|P?CaCl2|Usos del suelo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3426,"idioma_id":5,"titulo":". Caracterización del estado nutricional en relación con factores socio-ambientales de la población escolar del partido de Punta Indio (provincia de Buenos Aires, Argentina).","resumen":"El objetivo es evaluar el estado nutricional, la composición y proporción corporal en relación a las condiciones socio-ambientales de residencia de la población infanto-juvenil de las localidades de Verónica, Pipinas y La Viruta (partido de Punta Indio, provincia de Buenos Aires). Se realizó un estudio antropométrico transversal en 845 niños (47.5% varones y 52.5% mujeres) de 3 a 15 años. Se relevaron: peso, talla, estatura sentado, perímetro braquial y pliegues tricipital y subescapular y se calcularon indicadores nutricionales, de proporción y composición corporal, utilizando la referencia Third National Health and Nutrition Examination Survey. La información socio-ambiental se obtuvo aplicando encuestas estructuradas y fue procesada mediante Análisis de Componentes Principales Categórico. Esto permitió diferenciar cuatro grupos: I) ?urbano favorecido?; II) ?urbano intermedio?; III) ?urbano desfavorable? y IV) ?rural?. Para cada grupo se calcularon prevalencias de estado nutricional que fueron comparadas mediante prueba exacta de Fisher. Los resultados indicaron: 3.8% de desnutrición, 13.1% de sobrepeso y 10.9% de obesidad. La malnutrición por déficit y por exceso se vio acompañada por modificaciones en la composición corporal. El grupo ?rural? se diferenció de los otros grupos por no presentar casos de desnutrición crónica. Estos resultados permiten concluir que la población estudiada experimenta una transición nutricional avanzada, dada por bajas prevalencias de desnutrición y altas de sobrepeso y obesidad. Esta situación puede interpretarse en el marco de las transformaciones económicas intensas ocurridas recientemente en el partido de Punta Indio, las cuales no han conducido a erradicar la malnutrición sino a cambiar su polaridad hacia el exceso de peso.","palabras_clave":"Estado Nutricional |Transición nutricional|características socio-ambientales|Partido de Punta Indio, Buenos Aires"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3427,"idioma_id":4,"titulo":"Chaetophractus villosus as a sentinel organism: Baseline values of mitotic index, chromosome aberrations and sister chromatid exchanges","resumen":"Sentinel species are useful tools for studying the deleterious effects of xenobiotics on wildlife. The large hairy armadillo (Chaetophractus villosus) is the most abundant and widely distributed mammalin Argentina. It is a long-lived, omnivorous, burrowing species, with fairly restricted home ranges. To evaluate the level of spontaneous genetic damage in this mammal, we determined the baseline values ofseveral genotoxicity biomarkers. The study included 20 C. villosus adults of both sexes from eight pristine localities within its geographic distribution range. Genotoxicity analysis was performed on 72-h lymphocyte cultures, using mitomycin C as positive control. We obtained the baseline values of mitotic index (MI = 10.52 ± 0.30 metaphases\/total cells, n = 20), chromosome aberrations (CA = 0.13 ± 0.22, n = 20), sister chromatid exchanges (SCE) = 6.55 ± 0.26, n = 6) and replication index (RI = 1.66, n = 6). MI and CA did not show significant differences (P > 0.05) among localities or between sexes. No significant differencesin MI, CA, SCE, and RI (P > 0.05) were found between values from the pristine localities and historical data. There were significant differences in CA, SCE, and RI (P < 0.05) between lymphocyte cultures from pristine localities and those exposed to mitomycin C. We propose the large hairy armadillo as a sentinel organism for environmental biomonitoring of genotoxic chemicals due to its abundance, easy manipulation, well known biology, the fact that it is usually exposed to different mixtures and concentrations of environmental contaminants, and the baseline values of genetic damage characterized by MI, CA, SCEand RI as biomarkers.","palabras_clave":"CHAETOPHRACTUS VILLOSUS|SENTINEL ORGANISM|SISTER CHROMATID EXCHANGE|CHROMOSOME ABERRATIONS|BIOMARKERS|GENOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3428,"idioma_id":4,"titulo":"Plant diversity patterns and their conservation implications in neotropical dry forests","resumen":"Seasonally dry tropical forests are distributed across Latin America and the Caribbeanand are highly threatened, with less than 10% of their original extent remaining in manycountries. Using 835 inventories covering 4660 species of woody plants, we show markedfloristic turnover among inventories and regions, whichmay be higher than in other neotropicalbiomes, such as savanna. Such high floristic turnover indicates that numerous conservationareas across many countries will be needed to protect the full diversity of tropical dry forests.Our results provide a scientific framework within which national decision-makers cancontextualize the floristic significance of their dry forest at a regional and continental scale.","palabras_clave":"FLORISTIC|TURNOVER|NEOTROPICS|DRYFORESTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3429,"idioma_id":4,"titulo":"Oxidation\tof\tglycerol\twith\tH 2 O 2 \ton\tPb&#8208;promoted\tPd\/&#947;&#8208;Al 2 O 3 \t \t catalysts","resumen":"A series of bimetallic Pd&#8208;Pb catalysts with a constant Pd content of 1 wt% and Pb\/Pd atomic ratio from 0 to 1.6 supported on &#947;&#8208;Al2O3 were prepared and used for glycerol oxidation with H2O2 as the oxidizing agent at atmospheric pressure, 45°C and pH=11. The morphology and dispersion of the catalysts were characterized by scanning electron microscopy&#8208;energy dispersive X&#8208;ray spectroscopy (SEM&#8208;EDX) and transmission electron microscopy (TEM). The presence of an alloy phase in the bimetallic catalyst was detected by X&#8208;ray photoelectron spectroscopy (XPS). Glycerol conversion obtained with the monometallic Pd catalyst was 19%, which was increased to 100% with the addition of Pb. The four bimetallic PdPb catalysts were able to oxidize glycerol to dihydroxyacetone (DIHA) and the selectivity to DIHA reached 59%, 58%, 34% and 25% for PdPb0.25, PdPb0.50, PdPb1.00 and PdPb1.60 catalysts, respectively.","palabras_clave":"PALLADIUM|BIMETALIC PALLADIUM-LEAD CATALYST|HIDROGEN PEROXIDE|GLYCEROL OXIDATION|GLYCERIC ACID|GLYCOLIC ACID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3439,"idioma_id":4,"titulo":"Composites based on modified clay assembled Rh(III)- heteropolymolybdates as catalysts in the liquid-phase hydrogenation of cinnamaldehyde","resumen":"New composites based on the [RhMo6O24H6]3 (RhMo6) heteropolyanion supported on pillared (PILC), heterostructured (PCH) and functionalized(PILC-F) and (PCH-F) systems based on clays were prepared, characterized and tested as catalysts in the liquid-phase hydrogenation of cinnamaldehyde. The original phases and supported systems were characterized using several techniques such as powder X-ray diffraction (XRD), scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEMeEDS), Raman microprobe studies, X-ray photoelectron spectroscopy (XPS), thermogravimetry and differential thermal analyses (TG-DSC), temperature programmed reduction (TPR), and textural analysis (BET method), which confirmed their functionalization, physicochemicalmodification and the nature of Mo adsorbed species. Active acidic, basic and redox sites were determined by temperature programmed surface reaction (TPSR). Mo loading reached 7 wt% for the system RhMo6\/PCH-F and 3 wt% for the system RhMo6\/PILC-F, while unfunctionalized clay systems showed a value of 1 wt% of Mo. The catalytic performance showed that PCH-based composites were the most active and reached up to 56% conversion at 360 min of reaction when tested in liquid-phase cinnamaldehyde  Hydrogenation.The selectivity for all the systems was mainly toward hydrocinnamic aldehyde (HCAL) and reached 77% for the RhMo6\/PCH-F catalyst at 25% conversion.","palabras_clave":"Rh(III)-heteropolymolybdates |Clays Composites |Chemical modification |Liquid-phase hydrogenation Cinnamaldehyde"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3499,"idioma_id":4,"titulo":"Toxicity of AMPA to the earthworm Eisenia andrei Bouché, 1972 in tropical artificial soil","resumen":"Aminomethylphosphonic acid (AMPA) - one of glyphosate?s main metabolites - has been classifiedas persistent in soils, raising concern regarding the widespread use of glyphosate in agriculture andforestry. Glyphosate may have negative or neutral effects on soil biota, but no information is availableon the toxicity of AMPA to soil invertebrates. Therefore our aim was to study the effect of AMPA onmortality and reproduction of the earthworm species Eisenia andrei using standard soil ecotoxicologicalmethods (ISO). Field-relevant concentrations of AMPA had no significant effects on mortality in acuteor chronic assays. Except at the highest concentration tested, a significant biomass loss was observedcompared to controls in the chronic assay. The number of juveniles and cocoons increased with higherconcentrations of AMPA applied, but their mean weights decreased. This mass loss indicates highersensitivity of juveniles than adults to AMPA. Our results suggest that earthworms coming from parentsgrown in contaminated soils may have reduced growth, limiting their beneficial roles in key soilecosystem functions. Nevertheless, further research is needed to better understand the mechanismsunderlying the sublethal effects observed here.","palabras_clave":"-"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3516,"idioma_id":4,"titulo":"Soil  quality  indicators  selection  by  mixed  models  and  multivariate techniques  in  deforested  areas  for  agricultural  use  in  NW  of  Chaco, Argentina","resumen":"ABSTRACT Sol' quality (SQ) is a multidimensional concept based on the use of indicators. Indicators integrated assessment gives much more information than individual data and a select set of parameters; solve operational and economic aspects of measurement and monitoring. One of the most important effects of Land Use Change (LUC) is the alteration of original properties of native forest soil. Chaco province has no previous published studies on soil quality (SQ) for cleared Lands. In Argentina between '1956 and 2000s the most significant changes occurred in West Subhumid Chaco, where crops expansion and deforestation were the highest of the country. The objectives of this study were: (1) to select SQL in native forest soils cleared for agricultural use in an area of Chaco province under LUC without available information; (2) to obtain values from these indicators for this environment, in order to increase current knowledge about LUC effects. Results show that LUC caused a significant degradation in diese parameters compared with soil af pristine condition, affecting physical and chemical quality in a relatively short time. TOC was the most sensitive indicator and decreased between 40 and 11% in cleared sites at surface. The selection by mixed models in a first step and then by multivariate techniques was effective because some variables were more consistent te discrirninate in the complete dataset the unaltered. condition (NF) of the disturbed (CL), taking into account the inherent variability of sarnpling sites and depths. Selected indicators were: total organic carbon (TOC), particulate organic carbon (POC), total nitrogen (N), bullí density (13d), and EF (erodible fraction); high correlation between some of them would allow performing an even tight selection. These indicators varied in performance between studied depths. This SQL set is feasible to recommend as a tool for sol health diagnostic and monitoring, prior to granting management plans currently submitted within the legal framework of native forests. .0 2015 Elsevier B.V. Ali rights reserved.","palabras_clave":"Land use change|Soil quality|Deforestation|Subhumid regions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3517,"idioma_id":4,"titulo":"Granulocyte colony-stimulating factor (G-CSF) upregulates &#946;1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation","resumen":"Multiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of &#946;1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1\/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1\/2 and p38 MAPK. In addition, the increase of &#946;1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates &#946;1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.","palabras_clave":"G-CSF|&#946;1 Integrin|Migration |PI3K |MAPK |Swan71cells"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3518,"idioma_id":4,"titulo":"THE OUTER MEMBRANE PROTEIN OMPQ IS REQUIRED FOR MATURE BIOFILM FORMATION IN BORDETELLA BRONCHISEPTICA","resumen":"Bordetella bronchiseptica, an aerobic Gram-negative bacterium, is capable of colonizing the respiratory tract of diverse animals and chronically persists inside the hosts by forming biofilm. Most known virulence factors in Bordetella species are regulated by the BvgAS two-component transduction system. The Bvg-activated proteins play a critical role during host infection. OmpQ is an outer membrane porin protein which is expressed under BvgAS control. Here, we studied the contribution of OmpQ to the biofilm formation process by B. bronchiseptica. We found that the lack of expression of OmpQ did not affect the growth kinetics and final biomass of B. bronchiseptica under planktonic growth conditions. The &#916;ompQ mutant strain displayed no differences in attachment level and in early steps of biofilm formation. However, deletion of the ompQ gene attenuated the ability of B. bronchiseptica to form a mature biofilm. Analysis of ompQ gene expression during the biofilm formation process by B. bronchiseptica showed a dynamic expression pattern, with an increase of biofilm culture at 48&#8202;h. Moreover, we demonstrated that the addition of serum anti-OmpQ had the potential to reduce the biofilm biomass formation in a dose-dependent manner. In conclusion, we showed for the first time, to the best of our knowledge, evidence of the contribution of OmpQ to a process of importance for B. bronchiseptica pathobiology. Our results indicate that OmpQ plays a role during the biofilm development process, particularly at later stages of development, and that this porin could be a potential target for strategies of biofilm formation inhibition.","palabras_clave":"OMPQ|BORDETELLA|BIOFILM|VIRULENCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3519,"idioma_id":5,"titulo":"Riesgos climáticos para el cultivo de maíz en el centro-sudeste bonaerense: B. deficiencias de agua y rendimientos","resumen":"Deficiencias de agua y temperaturas extremas son factores limitantes en la producción de maíz que a menudo provocan importantes pérdidas económicas. Los impactos dependen del estado fenológico del cultivo cuando la adversidad ocurre, la intensidad y el tiempo de exposición. El objetivo del trabajo fue evaluar el riesgo climático para la producción de maíz en el centro-sudeste bonaerense, debido a la exposición a temperaturas extremas. Los escenarios agrícolas seleccionados para la evaluación combinaron seis fechas de siembra y tres ciclos de madurez en las localidades de Azul, Tandil, Balcarce y Mar del Plata. Las estaciones de crecimiento (serie 1971-2010) fueron simuladas mediante un modelo de desarrollo basado en la temperatura del aire. El riesgo de daño por heladas en estados tempranos del desarrollo del cultivo fue determinado, en cada localidad, a partir de la ocurrencia de la adversidad después de la fecha estimada del estado de cuatro hojas expandidas (V4) para cada fecha de siembra. El riesgo de heladas antes de la fecha de madurez fisiológica (R6) del cultivo fue determinado en cada localidad para los escenarios de fecha de siembra x ciclo. Se determinó la frecuencia relativa de días con temperatura máxima mayor a 30 &#61616;C durante la estación de crecimiento y en el período crítico para la definición de rendimiento. En siembras tempranas, siempre hay mayor riesgo de daños para el cultivo, debido a la ocurrencia de heladas tardías después de V4. A medida que se atrasa la siembra, el riesgo disminuye en toda la región. Balcarce presentó menor riesgo de heladas tardías. Los escenarios de siembras tardías y ciclos largos aumentan el riesgo de heladas durante el llenado de granos. En Azul y Tandil se observa mayor frecuencia de años con heladas antes de R6. Ambas localidades muestran una tendencia significativa a adelantar las heladas tempranas. El riesgo asociado a temperaturas elevadas es menor que el riesgo por heladas. Los escenarios evaluados muestran que el riesgo de exposición a temperaturas elevadas es mayor en Azul.","palabras_clave":"FECHAS DE SIEMBRA|CICLOS DE MADUREZ|TEMPERATURAS ELEVADAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3520,"idioma_id":4,"titulo":"Molecular characterization of Chlamydia pneumoniae in animals and humans from Argentina Genetic characterization of Chlamydia pneumoniae","resumen":"In this study, genetic diversity of Chlamydia pneumoniae was investigated and the relationships between sequences amplified of different sources, clinical conditions and geographical regions of central Argentina were established. Samples amplified were similar to human C. pneumoniae patterns and show the high clonality of the population.","palabras_clave":"Chlamydia pneumonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3524,"idioma_id":4,"titulo":"Tucumán ionospheric model (TIM): Initial results for STEC predictions","resumen":"Most ionospheric models can calculate vertical total electron content (VTEC) predictions, but only a few are suitable for calculating slant total electron content (STEC). This ionospheric magnitude is generally measured for electron content determinations, with VTEC particularly corresponding to an elevation of 90_. This is generally obtained by applying a mapping function to STEC measurements, which leads to important calculation errors. Moreover, the equatorial region has unique characteristics, such as the fountain effect and the equatorial electrojet, which lead to significant errors in the model?s calculations. In this paper, the Tucuma´n ionospheric model (TIM) is presented as a novel alternative for calculating the STEC in low-latitude regions (_24 to 24 dip latitude). The model is based on spatial geometry where the considered trajectory is segmented, and the corresponding electron density calculations for the resulting segment end points are determined using the semi-empirical low-latitude ionospheric model (SLIM) with reference to their corresponding magnetic coordinates and height. Finally, the electron density values are integrated along the path to obtain the STEC. This work describes the TIM and tests their STEC predictions for five ray paths around the world (totaling 16 cases under study), which are compared with experimental data from satellites and with those calculated by the NeQuick model. Moreover, the TIM performance for VTEC predictions is also checked and compared with VTEC data obtained from Global Positioning System (GPS) signals, IRI model, and NeQuick model predictions, for six GPS receiver stations during the equinox and solstice (totaling 12 cases studied). Comparisons of the TIM predictions with experimental data show that 53% of the calculation has, in general, deviations <30%. For the considered cases, TIM reproduces the experimental data better than the other models.","palabras_clave":"TEC|STEC |Equatorial anomaly"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3583,"idioma_id":4,"titulo":"Earthworm and Enchytraeid Co-occurrence Pattern in Organic and Conventional Farming: Consequences for Ecosystem Engineering","resumen":"Earthworms and enchytraeids are ecosystem engineers withan important influence on soil structure maintenance and nutrient cycling.We investigated if different agricultural managements produce a replacementof earthworms by enchytraeids, the magnitude of that replacement,and its effect on ecosystem engineering activities. Organic farming withplough tillage (ORG), conventional farming with plough tillage, conventionalfarming with no-tillage (NT), and unmanaged natural grasslands werestudied. Earthworms and enchytraeids were sampled by means of extractingand hand sorting soil monoliths. Soil bulk density, mechanical resistance, organicmatter content, and litter decomposition were measured as indicatorsof soil structure maintenance and nutrient cycling. A negative relation betweenearthworm and enchytraeid abundances was confirmed, not relatedto tillage intensity. Competitive interactions between them are suggested.Among agricultural systems, ORG had the highest earthworm abundanceand NT had the highest enchytraeids abundance and the highest enchytraeidto-earthworm ratio. Besides, intermediate abundances of earthworms andenchytraeids promoted by ORG were related to soil structure indicators´values similar to grassland and enhanced litter decomposition process. Despitea higher abundance of enchytraeids in NT, both soil structure maintenanceand nutrient cycling indicators had worse values than those in ORG.","palabras_clave":"EARTHWORMS|ENCHYTRAEIDS|NO TILLAGE|ORGANIC FARMING|SOIL FUNCTIONING|SUSTAINABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3589,"idioma_id":4,"titulo":"Effect of land use changes in eastern Amazonia on soil chemical, physical and biological attributes","resumen":"Land use change in rural settlements of the Amazon influences the abundance, diversity, and survival of soil fauna, especially earthworms, affecting the supply of ecosystem services. This study evaluated the effects of forest conversion to pasture on soil attributes and how the changes on the earthworm community affect soil porosity. Soil samples were collected from two toposequences (forest and pasture) at the summit (T1), midslope (T2) and footslope (T3) positions in July 2012 (dry season) and January and March 2013 (wet season). Samples were taken in five replicates at the depths of 0 to 10, 10 to 20, and 20 to 30 cm for determination of moisture, bulk density, total porosity, micromorphometry of pores, surface litter dry matter, chemical properties, and abundance and richness of earthworms. The numbers of macropores and micropores and S index (S) were calculated. The change on land use increased soil organic matter, pH, and calcium and reduced dry matter, moisture, and S index in pasture, as well as the loss of earthworm morphospecies highly related to the maintenance and formation of soil macroporosity, especially large rounded pores and, secondarily, large complex pores. This resulted in a loss of soil physical quality.","palabras_clave":"AMAZONIA|MACROPOROSITY|PORE MORPHOLOGY|SOIL BIOLOGY|SOIL FAUNA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3605,"idioma_id":4,"titulo":"Social Group Recommendation in the Tourism Domain","resumen":"Recommender Systems learn users´ preferences and tastes in differentdomains to suggest potentially interesting items to users. Group Recommender Systems generate recommendations that intend to satisfy a group of users as a whole, instead of individual users. In this article, we present a social based approach for recommender systems in the tourism domain, which builds a group prole by analyzing not only users´ preferences, but also the social relationships between members of a group.This aspect is a hot research topic in the recommender systems area. Inaddition, to generate the individual and group recommendations our approach uses a hybrid technique that combines three well-known filteringtechniques: collaborative, content-based and demographic filtering. Inthis way, the disadvantages of one technique are overcome by the others.Our approach was materialized in a recommender system named Hermes,which suggests tourist attractions to both individuals and groups of users.We have obtained promising results when comparing our approach withclassic approaches to generate recommendations to individual users andgroups. These results suggest that considering the type of users´ rela-tionship to provide recommendations to groups leads to more accuraterecommendations in the tourism domain. These findings can be helpfulfor recommender systems developers and for researchers in this area.","palabras_clave":"SOCIAL RECOMMENDER SYSTEMS|RECOMMENDER SYSTEMS|TOURISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3610,"idioma_id":4,"titulo":"Cyclosporine-A mimicked the ischemic pre- and postconditioning-mediated cardioprotection in hypertensive rats: Role of PKC&#949;","resumen":"Our aim was to assess the action of cyclosporine-A (CsA) against reperfusion injury in spontaneously hypertensive rats (SHR) compared to the effects of ischemic pre- (IP) and postconditioning (IPC), examining the role played by PKC&#949;. Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1 h reperfusion (R); IP: a cycle of 5 min GI and 10 min of R prior to 45 min-GI; and IPC: three cycles of 30 s-GI\/30 s-R at the start of R. Other hearts of the IC, IP and IPC groups received CsA (mitochondrial permeability transition pore inhibitor) or chelerythrine (Che, non-selective PKC inhibitor). Infarct size (IS) was assessed. TBARS and reduced glutathione (GSH) content ? as parameters of oxidative damage, the expression of P-Akt, P-GSK-3&#946;, P-PKC&#949; and cytochrome c (Cyc) release ? as an index of mitochondrial permeability and the response of isolated mitochondria to Ca2 + were also measured. IS similarly decreased in preconditioned, postconditioned and CsA treated heart showing the highest values in the combinations IP + CsA and IPC + CsA. TBARS decreased and GSH was partially preserved after all interventions. The content of P-Akt, P-GSK-3&#946; and P-PKC&#949; increased in cytosol and decreased in mitochondria after IP and IPC. In CsA treated hearts these enzymes increased in both fractions reaching the highest values. Cyc release was attenuated and the response of mitochondria to Ca2+ was improved by the interventions. The beneficial effects of IP and IPC were annulled when PKC was inhibited with Che. A PKC&#949;\/VDAC association was also detected. These data show that, in SHR, the CsA treatment mimicked and reinforced the cardioprotective action afforded by IP and IPC in which PKC&#949;-mediated attenuation of mitochondrial permeability appears as the main mechanism involved.","palabras_clave":"CsA|Ischemic preconditioning|Ischemic postconditioning|PKC&#949;|mPTP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3621,"idioma_id":4,"titulo":"Real-time Speckle Image Processing","resumen":"The laser dynamic speckle is an optical phenomenon produced when a laser light is re&#64258;ected from an illuminated surface undergoing some kind of activity. It allows a non-destructive process for the detection of activities that are not easily observable, such as seed viability, paint drying, bacterial activities, corrosion processes, food decomposition, fruit bruising, etc. The analysis of these processes in real time makes it possible to develop important practical applications of commercial, biological and technological interest. This paper presents a new digital system based on granular computing algorithms to characterize speckle dynamics within the time domain. The selected platform to evaluate the system is Field Programmable Gate Array (FPGA) technology. The obtained  minimum clock periods and latencies enable speckle image processing with real-time constraints with a maximum throughput of about thousand 512 9 512 fps.","palabras_clave":"Dynamic speckle|Granular computing |Programmable logic|Real-time processing|Rough-fuzzy sets"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3622,"idioma_id":4,"titulo":",?Multielemental composition and nutritional value of dumosa (Ilex dumosa), yerba mate (I. paraguariensis) and their commercial mixture in different forms of use?","resumen":"The multielement composition and nutritional value of 'dumosa' (Ilex dumosa) (ID),'yerba mate' (I. paraguariensis) (IP) and their commercial 70:30 mixture (ID:IP, w\/w) in crude drug, brewed mate and tea-bag-like infusion were studied by ICP-OES. Thismethod allowed the detection of an unprecedented number of elements for thesespecies. The order was K;Ca;Mg;P;Mn;Al;Na;Si;Fe in crude drug. IP showed higherconcentration of minerals than ID, but most extractability in the latter largely offset itsshortcomings. Brewed mate had higher nutritional value than mate tea because theirhigher solute\/solvent ratio. A portion of brewed mate of both species exceeded the dailyrecommended intake (DRI) of Mn, and providing Mg, Cu, Fe, Zn and K, too. The portions ofmate tea not contribute significantly to the DRI. Some essential trace elements and manyultra-traces ones that exist in crude drug were not detected in aqueous extracts. Most ofthe heavy metals were not leached into extracts, and in any case never exceeded internationalsafety limits. Therefore, the aqueous extracts of ID and IP and its mixture areinteresting from the nutritional point of view in minerales, and safe in terms of health.","palabras_clave":"Ilex dumosa|Ilex paraguariensis|nutritional value|mineral composition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3623,"idioma_id":5,"titulo":"Géneros de Hongos Necrofíticos Asociados a Hojas de Spartina Argentinensis y Panicum Prionitis (Poaceae)","resumen":"Los espartillares de Spartina argentinensis y los pajonales de Panicum prionitis, son comunidades herbáceas donde actualmente predominan sistemas de producción ganadera. En estas comunidades se acumula gran cantidad de biomasa senescente no aprovechable por el ganado por su baja digestibilidad, siendo la quema una práctica recurrentemente utilizada para estimular el rebrote de pasto más tierno. Este tipo de biomasa podría usarse para producir bioetanol. No obstante, para ello es necesario hacer un pretratamiento de la misma para aumentar la eficiencia del proceso de hidrólisis. Un posible pretratamiento es el uso de enzimas ligninolíticas que son comúnmente producidas por algunos hongos. En este trabajo, se relevaron los géneros fúngicos presentes en el residuo de hojas de ambas especies Poaceas, recolectadas en distintas localidades y con diferente grado de descomposición. Se encontró predominancia de géneros fúngicos citados por su capacidad ligninolítica. La mayoría de los mismos se encontraron en ambos hospedantes. A partir de estos hongos podrían obtenerse y aislarse enzimas ligninolíticas con las cuales mejorar la eficiencia de la producción de bioetanol lignocelulósico.","palabras_clave":"SPARTINA Y PANICUM|BIOETANOL  |BIOMASA LIGNOCELULÓSICA|MICOTA LIGNINOLÍTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3624,"idioma_id":4,"titulo":"'Quality indices of cooked southern king crab (Lithodes santolla Molina, 1782) meat during storage at 0 °C and -20 °C","resumen":"The aim of this work was to determine the quality changes of cooked Southern King Crab (Lithodes santolla) meat  during storage at 0 °C for 5 and 10 days and -20 °C for 10, 30, 60 and 90 days. Southern king crabs were obtained in the San Jorge gulf, Chubut, Argentina. Chemical indices varied from the initial to the final time in refrigerated and frozen samples as follows: TVB-N= 15.8-29.2mg% and 15.8-23.2 mg%, TMA= 0.0-8.3mg% and 0.0-1.2 mg%, formaldehyde: 0.5-8.6mg\/kg and 0.5-3.7 mg\/kg, indole: 3.3-20.7 µg% and 3.3-6.6 µg%, lactic acid: 16.4-39.1mg% and 16.4-16.6 mg%, and P-ratio (TMA\/TVB-N %)= 0-28.4% and 0-5.2%, respectively. The total nucleotide content rendered 6.6 µmol\/g, being AMP the main component. The nucleotide indices varied between the refrigerated and frozen cooked meat samples as follows: K from 7.37% to 27.5% and 10.3%; Ki from 58.3% to 86.5% and 82.7%; H from 30.2% to 54.90% and 38.0; G from 19.4% to 57.8% and 15.8%; P from 17.2% to 42.3% and 14.7%, and Fr from 41.6% to 13.4% and 17.3%, respectively. Biogenic amines found in both storage conditions were spermine, spermidine, tyramine and agmatine. Histamine and tryptamine were not detected, whereas 2-phenylethylamine was only identified during freezing. Spermine may be considered as a potential freshness index for refrigerated samples, and spermidine for frozen storage. Tyramine, which was not detected at initial time, reached values > 10mg% (which may cause migraine) under both storage conditions, it being advisable to monitor its level. During the storage period tested, none of the chemical indices studied exceeded the legal limits.","palabras_clave":"SOUTHERN KING CRAB|QUALITY|FRESHNESS|NUCLEOTIDES AMINAS BIOGENAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3625,"idioma_id":5,"titulo":"Correlación entre estrés, tabaquismo y estado periodontal en adultos","resumen":"Las enfermedades periodontales son infecciones asociadas a microorganismos patogénicos y alteraciones en la respuesta del huésped. Los factores psicosociales como el estrés y las conductas de afrontamiento inadecuadas pueden ejercer efectos inmunosupresivos, incrementando la susceptibilidad a periodontitis.         El objetivo del estudio observacional, transversal, fue investigar la asociación entre estrés psicosocial, conductas adaptativas y tabaquismo con el estado periodontal. Se  reclutaron 166 sujetos voluntarios de ambos sexos, sanos sistémicamente, con una media de edad de 41,2 ± 11,3 años, entre 25 a 65 años, que asistieron a la Facultad de Odontología de la UNCuyo. Para la valoración del estrés y conductas adaptativas se aplicaron: Escala de Reajuste Social (SRRS) Holmes y Rahe, Inventario de Síntomas de Estrés (SSI) Lipp y Guevara, cuestionario de conductas de afrontamiento (COPE) Carver. Se determinó el nivel de cortisol salival por radioinmunoensayo y se evaluó el hábito tabáquico. Se evaluaron los siguientes parámetros periodontales: profundidad de sondaje, pérdida de inserción clínica, índice gingival y de placa.         El estudio estadístico incluyó análisis de regresión logística bivariado y multivariado, prueba de chi cuadrado, OR IC 95%, y ANOVA.         Los resultados obtenidos demuestran asociación significativa por análisis bivariado: edad y estado periodontal (p         Los sujetos que presentan estrés, inadecuadas conductas adaptativas y fuman son más susceptibles al deterioro periodontal. Valores altos de cortisol salival se asociaron a mayor deterioro periodontal.       ","palabras_clave":"ENFERMEDAD PERIODONTAL|ESTRES PSICOSOCIAL|TABAQUISMO|CONDUCTAS DE AFRONTAMIENTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3626,"idioma_id":4,"titulo":"Occurrence and toxicity of Microcystis aeruginosa (Cyanobacteria) in the Paraná River, downstream of the Yacyretá dam (Argentina)","resumen":"Cyanobacteria constitute the main toxin producers in inland water ecosystems and have extensive global distribution. The presence of hepatotoxins in aquatic environments is hazardous to human and animal health; even though the presence and identification of hepatotoxic microcystins in rivers and reservoirs of the world have been confirmed by several studies in the last few years. Herein, we studied the abundance and toxicity of Microcystis aeruginosa in the Argentine section of the Paraná River at the beginning of the Middle Paraná (Corrientes Hydrometer), near Corrientes city (27º28´ S-58º51´ W) and approximately 220km downstream of the Yacyretá dam (High Paraná). The Paraná River basin, with a drainage area of 3.1 x 106km2 and 3 965km in length, is the second largest catchment of South America, after that of the Amazon. The Paraná River is the main source of drinking water supply for the Northeastern Argentine region. Phytoplankton samples were collected and environmental variables were measured in a monthly basis (exceptionally fortnightly), from March 2004 to June 2008. Fifty-eight samples were analyzed for phytoplankton density and biomass. Five samples were used for toxicity testing; the latter were obtained during the cyanobacteria blooms from 2005 to 2008. Phytoplankton counts were performed with an inverted microscope, and biomass was expressed as biovolume. Bioassays with mice and high-performance liquid chromatography (HPLC) analysis were performed to evaluate the presence of cyanotoxins. Phytoplankton mainly consisted of Cryptophyta, Chlorophyta and Bacillariophyta. Microcystis aeruginosa was identified during the warmer months each year (November to March). Density varied between 189 and 25 027cells\/mL (1-10 colonies\/mL) and biomass from 0.34 to 44mm3\/L. Taking into account the number of cells, the highest abundance occurred in April 2004 (25 027cells\/mL), coinciding with the largest biovolume (44mm3\/L). All mice subjected to intraperitoneal injections with samples obtained during bloom episodes showed positive results for the presence of hepatotoxins. Three microcystins variants: LR, RR and [D-Leu1] Mcyst?LR were detected by analysis with semi-preparative high-performance liquid chromatography with diode array detector system (HPLC-PDA). This constitutes the first report of microcystins recorded during M. aeruginosa blooms in the Argentine stretch of the Paraná River at the beginning of the Middle Paraná (Corrientes Hydrometer), approximately 220km downstream of the Yacyretá dam (High Paraná).","palabras_clave":"MICROCYSTIS AERUGINOSA |HEPATOTOXINS|MICROCUSTINS|YACYRETA DAM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3627,"idioma_id":4,"titulo":"The evolution of CSR?s research in tourism context: A review from 1992 to 2012","resumen":"Considering that Corporate Social Responsibility is a recurrent research topic in the marketing literature, this study aimed to analyze the epistemological orientation evolution of CSR research at tourism context during the last twenty years (1992-2012).  The abstracts of 101 CSR papers published on the most- impacting journal of tourism were classified by their epistemological orientation throughout the application of a content analysis technique.   The results show a significant increment of CSR?s literature in tourism during the time-period analyzed. In addition, the theoretical contributions prevail over the prescriptive and descriptive ones. A differentiation in terms used to refer to CSR topic is also evidenced, as ?CSR? and ?social responsibility? are keywords used at enterprises papers, while ?responsible tourism? is preferred at destinations\/industry studies.  This paper conclusion suggests that the research on CSR in tourism is following a progressive evolution. Besides, its increment is continuing due to the proliferation of exploratory studies and, lesser extent, to the predictive studies. Attending to the limited number of journals analyzed in this study, an extension of the review to lower impact journals of tourism is suggested.","palabras_clave":"Corporate Social Responsibility |Responsible Tourism |Epistemological evolution |Tourism literature|Content analysis |Bibliometric"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3628,"idioma_id":4,"titulo":"Chemometrics Classification of Argentine Clover and Eucalyptus Honeys According to Palynological, Physicochemical and Sensory Properties","resumen":"Palynological, physicochemical and sensory methods were applied to ascertain the most discriminant variables for honey characterization. Fifteen physicochemical parameters, six indicators of antioxidant capacity and eight sensory attributes were considered. Clover and eucalyptus honeys were differentiated when the linear discriminant analysis technique was applied. Electrical conductivity, sugars, ferric ion reducing and Trolox equivalent antioxidant capacity were the most discriminant variables. Odor and color intensities were the sensory attributes scoring the major differences between honeys. The canonical correlations technique pointed out that differences were related primarily to the content of pollen from Trifolium, Medicago sativa and Lotus. These standards of quality provide a differentiating tool to classify unifloral honeys.","palabras_clave":"CLOVER HONEY|TRIFOLIUM HONEY|CHEMOMETRICS CLASSIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3629,"idioma_id":4,"titulo":"Validation of a sampling plan to generate food composition data","resumen":"A methodology to develop systematic plans for food sampling was proposed. Long life whole and skimmed milk, and sunflower oil were selected to validate the methodology in Argentina. Fatty acid profile in all foods, proximal composition, and calcium?s content in milk were determined with AOAC methods. The number of samples (n) was calculated applying Cochran?s formula with variation coefficients &#10877;12% and an estimate error (r) maximum permissible &#10877;5% for calcium content in milks and unsaturated fatty acids in oil. n were 9, 11 and 21 for long life whole and skimmed milk, and sunflower oil respectively. Sample units were randomly collected from production sites and sent to labs. Calculated r with experimental data was &#10877;10%, indicating high accuracy in the determination of analyte content of greater variability and reliability of the proposed sampling plan. The methodology is an adequate and useful tool to develop sampling plans for food composition analysis.","palabras_clave":"SAMPLING PLAN|METHODOLOGY VALIDATION|FOOD COMPOSITION|VARIABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3630,"idioma_id":4,"titulo":"Essential oil of Aloysia triphylla as feed additive promotes growth of silver catfish (Rhamdia quelen)","resumen":"This study examined the growth, morphology and occurrence of endocrine cells in the intestines of juvenile silver catfish (Rhamdia quelen) fed diets supplemented with differing amounts of essential oil (EO) extracted from the verbena Aloysia triphylla (0 ? control, 0.25, 0.5, 1.0 and 2.0 mL EO per kg of diet) for 60 days. The group fed 2.0 mL EO per kg of diet showed better results in growth (body weight, weight gain and specific growth rate) after 60 days. Histological and immunohistochemical analyses of the intestine demonstrated that height of folds was significantly higher in the control group and the 0.25 mL EO per kg of diet group compared to those fed 0.5 mL EO per kg of diet. There was a significant increase in the number of folds in fish fed 1.0 and 2.0 mL EO per kg of diet compared to the other groups. The enterocyte height was significantly lower in those fed 1.0 mL of EO per kg of diet group compared with the 0.25 mL of EO per kg of diet group. The number of cells that reacted to cholecystokinin and neuropeptide Y did not differ between groups. The addition of 2.0 mL EO per kg of diet increases silver catfish growth, and consequently, its use as a feed additive is recommended.","palabras_clave":"DIGESTIVE TRACT|IMMUNOHISTOCHEMISTRY|FOOD ADITIVE|DIET"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3631,"idioma_id":5,"titulo":"TV, ciencia y tecnología: una transformación mutua","resumen":"Este trabajo se desprende del proyecto de investigación Análisis del diseño y de los formatos audiovisuales en la divulgación científica, cuyo objetivo es el análisis de las continuidades, innovaciones y tendencias en la programación de Ciencia y Tecnología (CyT) dentro de la configuración televisiva argentina actual para interpretar la influencia del medio en  la construcción del imaginario social sobre CyT.    Desde esta perspectiva el presente artículo se propone contrastar los rasgos del discurso televisivo y los del discurso de comunicación de la ciencia y tecnología para analizar a nivel estético-narrativo tres programas de CyT que coexisten en la pantalla actual: Sudamérica Salvaje, TN Ciencia y Mentira la verdad, seleccionados de un corpus de 362 programas analizados (2011\/2013). A partir de este recorte se pretende dar cuenta de las estrategias comunicativas de CyT en una configuración televisiva en intensa transformación. El análisis en torno a las formas de narrar\/mostrar y los contenidos en los programas sobre CyT no sólo urge en tanto reflexión teórica, sino que, teniendo en cuenta la coyuntura actual a partir de la creciente implementación de Ley de Servicios de Comunicación Audiovisual (Ley n° 26.522), se torna necesario a la hora de repensar la producción audiovisual.","palabras_clave":"hipertelevisión|divulgación de C y T|formatos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3632,"idioma_id":4,"titulo":"INTER-  ANNUAL VARIABILITY IN PROSOPIS CALDENIA POD PRODUCTION IN THE  ARGENTINEAN SEMIARID PAMPAS: A MODELLING APPROACH.","resumen":"The driest part of the Argentinean pampasis occupied by semiarid woodlands dominates by Prosopis caldenia Burkart(Calden). Calden pods are a highly valuable fodder supplement for livestock butits production is highly variable. Our objective was to analyze and model thetemporal pattern in inter-annual variability of Calden pod production. Our keyhypothesis is that weather conditions are the main determinant of the podmasting behaviour. Tree size and climatic variables were evaluated as explanatorycovariates using a zero-in&#64258;ated log-normal modelling approach. The proposed&#64257;nal model structure incorporated 25 parameters, including four variancecomponents, two intercepts for both the logistic and the log-normal parts ofthe model, and nineteen parameters associated with &#64257;xed effects.Climate had astrong in&#64258;uence on the &#64258;owering-fruiting Calden process and on the inter-annualvariability of the &#64257;nal pod production at the tree level. Temperatures duringbud breaking, &#64258;owering and fruit shedding, together with the precipitation fromthe &#64257;nal month of fruit shedding and the total amount of the prior vegetativecycle, were the main weather covariates that affect the processes.","palabras_clave":"PROSOPIS|RANGING|FOREST MANAGEMENT|SEMIARID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3633,"idioma_id":-1,"titulo":"Nutritional improvement of corn pasta-like product with broad bean (Vicia faba) and quinoa (Chenopodium quinoa)","resumen":"In this study, the nutritional quality of pasta-like product(spaghetti-type), made with corn (Zea mays) flour enriched with 30% broad bean(Vicia faba) flour and 20% of quinoa (Chenopodium quinoa) flour, wasdetermined. Proximate chemical composition and iron, zinc and dietary fiberwere determined. A biological assay was performed to assess the protein valueusing net protein utilization (NPU), true digestibility (TD) and proteindigestibility-corrected amino acid score (PDCAAS). Iron and zinc availability wereestimated by measuring dialyzable mineral fraction (%Da) resulting from invitro gastrointestinal digestion. Nutritionally improved, gluten-free spaghetti(NIS) showed significantly increased NPU and decreased TD compared with anon-enriched control sample. One NIS-portion supplied 10?20% of recommended fiberdaily intake. Addition of quinoa flour had a positive effect on the FeDa% asdid broad bean flour on ZnDa%. EDTA increased Fe- and ZnDa% in allNIS-products, but it also impaired sensorial quality.","palabras_clave":"DIALYZABILITY|IRON|ZINC|PROTEIN DIGESTIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3634,"idioma_id":4,"titulo":"E-cadherin roles in animal biology. A perspective on thyroid hormone-influence","resumen":"The establishment, remodeling and maintenance of tissular architecture during animal development, and even across juvenile to adult life, are deeply regulated by a delicate interplay of extracellular signals, cell membrane receptors and intracellular signal messengers. It is well known that cell adhesion molecules (cell-cell and cell- extracellular matrix) play a critical role in these processes. Particularly, adherens junctions (AJs) mediated by E- cadherin and catenins determine cell-cell contact survival and epithelia function. Consequently, this review seeks to encompass the complex and prolific knowledge about E-cadherin roles during physiological and pathological states, particularly focusing on the influence exerted by the thyroid hormone (TH).","palabras_clave":"E-cadherin|Adherens junction|Epithelia|Development|Evolution|Thyroid hormones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3635,"idioma_id":5,"titulo":"Parámetros fisicoquímicos e isotermas de adsorcion de hojas de Maytenus ilicifolia Martius","resumen":"Maytenus Ilicifolia Martius (Celastraceae) es una especie nativa en la Provincia de Misiones. Con sus hojas secasse preparan diferentes infusiones y con el objeto de estandarizar las mismas se estudiaron sus parámetrosfisicoquímicos e isotermas de adsorción.El contenido de humedad de equilibrio fue medido usando un método gravimétrico estático, a humedades relativasy temperaturas en un rango de 11,0% a 80,3% y de 30 ºC a 60 ºC respectivamente. Los modelos matemáticos deGuggenheim-Anderson-de Boer (GAB), Chung-Plost, Halsey modificado, Oswin modificado, Henderson modificadoy Halsey fueron utilizados para ajustar los datos experimentales.Los ensayos de cenizas totales, pérdida por secado y extractos acuosos cumplen con Farmacopea Brasilera. Losvalores óptimos obtenidos por TLC para los triterpenos fueron con la fase móvil cloroformo-benceno con Rf de 0,23,0,38, 0,57, 0,80 y 0,96. Los datos experimentales de adsorción exhiben una curva sigmoidea Tipo II. Los mejoresajustes se obtuvieron con la ecuación de GAB","palabras_clave":"Parámetros fisicoquímcos|Isotermas de adsorción|Maytenus Ilicifolia|Modelos matemáticos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3636,"idioma_id":4,"titulo":"EPIDIDYMIS OF VISCACHA (Lagostomus maximus maximus): A MORPHOLOGICAL COMPARATIVE STUDY IN RELATION TO SEXUAL MATURITY","resumen":".","palabras_clave":"Epididymis |Testis |Viscacha"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3637,"idioma_id":5,"titulo":"Habilidades sociales: Su importancia en mujeres con diagnóstico de Síndrome de Turner","resumen":"La Psicología Positiva postula la importancia de trabajar desde una mirada integradora de cada persona incluyendo sus fortalezas y debilidades. Esta mirada resulta importante al trabajar con poblaciones físicas, psicológicas y socialmente vulnerables. El Síndrome de Turner es un trastorno cromosómico determinado por la deleción del cromosoma X en el sexo femenino, siendo la talla y la disgenesia gonadal sus principales características físicas. Las mismas tienen un gran impacto en el desarrollo psicológico de esta población generando problemas en la capacidad de establecer relaciones sociales óptimas. El objetivo del presente trabajo es referenciar la importancia del desarrollo de habilidades sociales en el Síndrome de Turner, principalmente durante la niñez y la adolescencia, considerando que las mismas operan como un recurso salugénico para evitar trastornos mayores en la vida adulta.","palabras_clave":"SÍNDROME DE TURNER|HABILIDADES SOCIALES|PSICOLOGÍA POSITIVA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3638,"idioma_id":5,"titulo":"Cinco años de experiencia en conservación de Streptococcus agalactiae: comparación de dos metodologías alternativas","resumen":"Streptococcus agalactiae (GBS) is responsible for severe neonatal disease and other pathologies. It is necessary its preservation for research involving: search for virulence and antimicrobial resistance genes, epidemiological studies, biotechnological developments and shipments to referral centers. The aim of this study was to compare two methods of preservation: Trypticase Soy Agar (ATS-S) to 7 ? at room temperature and darkness and skim milk and 20% at -80ºC (LD-80). One hundred and fifty strains were evaluated at three, twelve to sixty months. After shelf viability, purity, antigenic and genetic stability were verified. For each period studied, recovery rates were: 100%, 80% and 15.33% for ATS-S and 100%, 100% and 98.66% for LD-80. From 12 months significant differences (p <0.05) were found between the two methods. We conclude that LD-80 is highly effective to maintain GBS strains with percentages nearby recovery to 100%.","palabras_clave":"Streptococcus agalactiae|Agar tripteina soya|Leche descremada"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3639,"idioma_id":4,"titulo":"Optimization in the diagnosis of Trichomonas vaginlis infection during   pregnancy in an University Hospital?.","resumen":"Abstract: The aim of this study was to evaluate different methods for Trichomonasvaginalis diagnosis during pregnancy. A total of 386 vaginal exudates from pregnantwomen were analyzed. T. vaginalis was investigated by different microscopicexaminations (direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining and wet mount with sodium-acetate-formalin (SAF)\/methylene blue). Polymerase chain reaction (PCR) for RNA 18S gene as well as culturein liquid medium (modified thioglycolate medium) were performed. The sensitivity andspecificity of the microscopic examinations were evaluated considering the culturemedia positivity or the PCR techniques as gold standard. The frequency of T. vaginalisinfection was 6.2 % by culture and\/or PCR, 5.2 % by PCR, 4.7 % by culture, 3.1 % bySAF\/ methylene blue method and 2.8% by direct wet smear and prolonged MGGstaining. The sensitivities were 83.3%, 75%, 50% and 45.8% for PCR, culture, SAF\/methylene blue method, direct wet smear and prolonged MGG staining respectively.The specificity of all methods was 100%. Microscopic examinations showed lowsensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve thedetection of T. vaginalis using combined methods providing higher sensitivity such asculture and PCR, mainly in asymptomatic pregnant patients, in order to preventmaternal and perinatal complications.","palabras_clave":"DIAGNOSIS|TRICHOMONAS|PREGNANCY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3640,"idioma_id":4,"titulo":"Reproductive biology of the southernmost Kentropyx lizard from the Wet Chaco of Corrientes, Argentina","resumen":"Studies on lizards reproduction, determining the reproductive mode, size at maturity, clutch size, and clutch frequency, have contributed greatly to understand factors influencing life history variation among populations, and thereby, to implement conservation strategies. Herein, we studied the reproductive biology of the vulnerable tegu lizard Kentropyx viridistriga from Corrientes, northeastern Argentina (Wet Chaco). We found that temperature and photoperiod influence the timing of reproduction and the brumation period of K. viridistriga which are active from early summer to late autumn. Females showed a seasonal and annual reproductive cycle and males exhibited a continuous reproductive cycle during the activity season. Females lay at least two clutches of on average three eggs per reproductive season. Neonates occur in spring, and reach sexual maturity in the first year of life. Females reached sexual maturity at a larger snout-vent length (64.1 mm) than males (54.8 mm). Sexual dimorphism is evident, with males showing greater head size and longer tails than females, while females exhibit larger interlimb length than males. This study reveals that the reproductive strategy of K. viridistriga is adjusted to the environmental conditions of the southernmost distribution for the genus.","palabras_clave":"Reproductive cycle|life history|Squamata, Teiidae|Kentropyx viridistriga"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3641,"idioma_id":4,"titulo":"Cr\/SiO2 mesoporous catalysts: Effect of hydrothermal treatment and calcination temperature on the structure and catalytic activity in the gas phase dehydration and dehydrogenation of cyclohexanol","resumen":"Cr\/SiO2 mesoporous materials, (Cr-MCM-41), were obtained by hydrothermal synthesis. The effect of hydrothermal treatment and calcination temperature on the structure and catalytic behavior of synthesized materials were analyzed. The materials were characterized by XRD, N2 adsorptiondesorption,TEM-SEM, TG-DTA, FTIR-HATR, DRUV-Vis and XPS. The hydrothermal treatment promoted the incorporation of Cr3+ into the silica network, improving their resistance to oxidation. The calcination in air produces the simultaneous presence of Cr3+ and Cr6+ ions, and their relative amounts depend both on the hydrothermal pretreatment and the calcination temperatures. The Cr3+ ions constituted coordinative unsaturated acid centers responsible of the cyclohexanol to cyclohexene dehydration reaction. Moreover, the Cr6+ ions correspond to either, isolated monochromate species linked to the silica structure, or to polychromate aggregates; which produce crystalline &#945;-Cr2O3 clusters after calcination in air to 450°C. The monochromate centers were active for the dehydrogenation reaction. So, although dehydration was the main reaction, the dehydrogenation reaction to cyclohexanone also was observed after calcination in air to 450 °C.","palabras_clave":"Cr\/SiO2|MCM-41|cyclohexanol|Dehydrogenation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3642,"idioma_id":4,"titulo":"DEVELOPMENT OF CARBON FIBER\/PHENOLIC RESIN PREPREGS MODIFIED WITH NANOCLAYS","resumen":"This work is focused on the study of the main variables involved in the development of phenolic\/carbon prepregs and how these variables are affected by the incorporation of nanoclays. For this, phenolic resin was obtained from phenol and formaldehyde, performing the reaction under basic conditions and with formaldehyde excess. Resin curing kinetics was studied by means of Fourier Transform Infrared Spectroscopy (FTIR) measurements. Organo-modified clay was added to the phenolic resin in order to evaluate their effect on the general performance of the material. Carbon fiber-phenolic resin and carbon fiber-clay modified phenolic resin prepregs were obtained by hand-layup and vacuum bagging. Prepregs were characterized in terms of fiber content, flexural stiffness and degree of tack. In both cases, intermediate fiber volume content, near to 50 %, was obtained. The addition of 5 wt. % of clay to the phenolic resin did not produce a significant change on the prepregs stiffness but increased the degree of tack, which implies that the incorporation of such particles was useful to enhance the prepregs properties. Composite materials were obtained by compression molding of the prepregs obtained, and were characterized by its fiber content, mechanical behavior with the three point bending test and its thermal degradation by thermogravimetry. High fiber content composites materials were obtained, near to 75 % by volume. However modified composites showed lower fiber content, which resulted in a decrease of the flexural modulus and strength. In relation to thermal degradation the addition of nanoclay did not change the behavior of the materials.","palabras_clave":"PREPREGS|NANOCLAYS|PHENOLIC COMPOSITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3643,"idioma_id":4,"titulo":"Water  deficit  stress  tolerance  in  maize  conferred  by  expression  of  an isopentenyltransferase  (IPT)  gene  driven  by  a  stress-  and maturation-induced  promoter","resumen":"tSenescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT)due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photo-synthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate andcharacterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence underthe regulation of SARK promoter (protein kinase receptor-associated senescence). Three independenttransgenic events and their segregating null controls were evaluated in two watering regimes (WW:well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WDtreatment induced IPT expression with the concomitant increase in cytokinin levels, which prolongedthe persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal con-ductance. These trends were accompanied by a minor decrease in number of grains per plant, individualgrain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WDhad PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstratethat expression of the IPT gene under the regulation of the SARK promoter helps improve productivityunder WD conditions in C4plants like maize.","palabras_clave":"WATER STRESS TOLERANCE|ZEA MAYS|CYTOKININ|PLANT GRAIN YIELD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3644,"idioma_id":4,"titulo":"Diversity and richness of small mammals at a well-conserved site of The Yungas in Jujuy Province, Argentina","resumen":"We present the results obtained from 12 smallmammal surveys conducted between 1996 and 2013 in ? Finca Las Capillas ? , province of Jujuy, Argentina. This region has been formally recognized as an ? Area of Importance for the Conservation of Bats ? (AICOM) based on the diversity of species detected in our previous studies. This site in the Yungas forests of northwestern Argentina still maintains an excellent conservation status due to restrictions to human activities involving deforestation and limitations to cattle-raising imposed 50 years ago. A total of 39 species belonging to three orders, namely, Didelphimorphia, Chiroptera and Rodentia, were recorded. The species accumulation curve revealed that our records represent 83 ? 95% of the community, and with high diversity indexes. Several of the genera and species that we recorded from our collection trips have been included in the fauna of Argentina. Our previous studies and the present findings provide new records for the province or significant extensions to previously known distributions of several taxa of small mammals. These results show that the site represents an icon for conservation in such a fragmented and exploited area as the Yungas in Argentina today.","palabras_clave":"Chiroptera|conservation|Didelphimorphia|Rodentia|Yungas forest"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3645,"idioma_id":5,"titulo":"EL USO DE RECURSOS DIGITALES PARA LA COMUNICACIÓN PÚBLICA DE LA ARQUEOLOGÍA","resumen":"        Se reflexiona sobre la comunicación pública  de la ciencia mediante recursos digitales a partir del análisis de la  experiencia de organización y diseño del sitio web  ?Arqueología Pampeana del Río Salado?. Este sitio es parte de una estrategia comunicativa desarrollada por nuestro  equipo de arqueología con el propósito de comunicar los resultados de  investigación en las localidades donde las mismas se desarrollan. Las preguntas  que guían este trabajo consideran el tipo de relaciones con el conocimiento que  se movilizan mediante un sitio web de arqueología y en qué medida los entornos  virtuales son espacios que pueden propiciar modelos participativos para la  comunicación de la ciencia.  ABSTRACT         This paper considers the  public communication of science through digital resources based on an analysis  of the experience of organization and design of the 'Arqueología Pampeana  del Río Salado' website. This site integrates a communication strategy  developed by our archaeology team in order to communicate researches results in  the localities where they are developed. The questions that guide this work take  into account the kinds of relationships with the knowledge that move through an  archaeology website and the discussion about how virtual environments could be spaces  to promote participatory models for science communication.        KEYWORDS: Public communication of science, Archaeology, Web 2.0,  Information architecture.  ","palabras_clave":"COMUNICACIÓN |ARQUEOLOGÍA|ARQUITECTURA DE LA INFORMACIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3646,"idioma_id":4,"titulo":"Metabolic profiling of a range of peach fruit varieties reveals high metabolic diversity and commonalities and differences during ripening","resumen":"Peach (Prunus persica) fruits from different varieties display differential organoleptic and nutritionalproperties, characteristics related to their chemical composition. Here, chemical biodiversity of peachfruits from fifteen varieties, at harvest and after post-harvest ripening, was explored by gas chromatography?massspectrometry. Metabolic profiling revealed that metabolites involved in organoleptic properties(sugars, organic and amino acids), stress tolerance (raffinose, galactinol, maltitol), and withnutritional properties (amino, caffeoylquinic and dehydroascorbic acids) displayed variety-dependentlevels. Peach varieties clustered into four groups: two groups of early-harvest varieties with higher aminoacid levels; two groups of mid- and late-harvest varieties with higher maltose levels. Further separationwas mostly dependent on organic acids\/raffinose levels. Variety-dependent and independent metabolicchanges associated with ripening were detected; which contribute to chemical diversity or can be usedas ripening markers, respectively. The great variety-dependent diversity in the content of metabolitesthat define fruit quality reinforces metabolomics usage as a tool to assist fruit quality improvement inpeach.","palabras_clave":"Prunus persica| Fruit |Metabolic profiling|Metabolic diversity |Ripening| Varieties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3647,"idioma_id":4,"titulo":"Network and biosignature analysis for the integration of  transcriptomic and metabolomic data to characterize leaf senescence process in sunflower","resumen":"Background: In recent years, high throughput technologies have led to an increase of datasets from omicsdisciplines allowing the understanding of the complex regulatory networks associated with biological processes.Leaf senescence is a complex mechanism controlled by multiple genetic and environmental variables, which hasa strong impact on crop yield. Transcription factors (TFs) are key proteins in the regulation of gene expression,regulating different signaling pathways; their function is crucial for triggering and\/or regulating different aspectsof the leaf senescence process. The study of TF interactions and their integration with metabolic profiles underdifferent developmental conditions, especially for a non-model organism such as sunflower, will open new insightsinto the details of gene regulation of leaf senescence.Results: Weighted Gene Correlation Network Analysis (WGCNA) and BioSignature Discoverer (BioSD, GnosisData Analysis, Heraklion, Greece) were used to integrate transcriptomic and metabolomic data. WGCNA allowed thedetection of 10 metabolites and 13 TFs whereas BioSD allowed the detection of 1 metabolite and 6 TFs as potentialbiomarkers. The comparative analysis demonstrated that three transcription factors were detected through bothmethodologies, highlighting them as potentially robust biomarkers associated with leaf senescence in sunflower.Conclusions: The complementary use of network and BioSignature Discoverer analysis of transcriptomic andmetabolomic data provided a useful tool for identifying candidate genes and metabolites which may have a roleduring the triggering and development of the leaf senescence process. The WGCNA tool allowed us to designand test a hypothetical network in order to infer relationships across selected transcription factor and metabolitecandidate biomarkers involved in leaf senescence, whereas BioSignature Discoverer selected transcripts andmetabolites which discriminate between different ages of sunflower plants. The methodology presented herewould help to elucidate and predict novel networks and potential biomarkers of leaf senescence in sunflower.Keywords: WGCNA, BioSignature Discoverer, Leaf senescence, Transcriptomic, Metabolomic, Data integration,Sunflower","palabras_clave":"Senescence|sunflower|data integration|transcriptomic|metabolomic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3648,"idioma_id":4,"titulo":"Evidence for independent instances of chromosome number reduction in the genus Pseudopaludicola (Anura: Leptodactylidae)","resumen":"We describe the chromosome morphology, C-bands, NORs position, and DAPI\/CMA3 staining of five species of Pseudopaludicola (P. boliviana, P. canga, P. falcipes, P. mystacalis, and P. ternetzi) from several populations from Argentina, Brazil, Paraguay, and Uruguay. In addition, the telomeric sequence locations of P. mystacalis and P. canga are evaluated using FISH. We re-analyse the progressive reduction of chromosome numbers recently proposed for the genus by optimisation of character basic chromosome numbers (x) and fundamental chromosome numbers (FN) on current phylogenetic hypotheses. Our evidence supports a progressive reduction of chromosome numbers in Pseudopaludicola, and multiple origins of the karyotype 2n = 20. Additionally, we discuss the interpretation of the current Pseudopaludicola phylogenetic hypothesis and a series of inconsistencies in the chromosome literature for the genus.","palabras_clave":"C-bands|Chromosome reduction|CMA3|Character Optimisation|DAPI|NORs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3649,"idioma_id":4,"titulo":"STARCH EDIBLE FILM SUPPORTING NATAMYCIN AND NISIN FOR IMPROVING MICROBIOLOGICAL STABILITY OF REFRIGERATED ARGENTINIAN POR SALUT CHEESE","resumen":"The effectiveness of natamycin and nisin supported in edible films (NANI) was evaluated, at 7 ± 1 ºC, in relation to the improvement of the microbiological stability of Port Salut cheese. This film inhibited the growth of yeasts and moulds and controlled the growth of psychrotrophic bacteria originally present in the Port Salut cheese stored at refrigeration temperature. It also inhibited the development of a mixed culture (Saccharomyces cerevisiae and Listeria innocua) present in the cheese due to a superficial contamination, throughout the entire storage. With respect to a postprocessing contamination affecting the film present in a covered cheese, control film, commercial film and film containing nisin and natamycin (NANI), acted as barriers precluding the mixed culture growth on the cheese surface. But only NANI inhibited the growth of that culture on the film.These results are demonstrating that the film NANI is an extremely effective method to control the population of microorganisms present in both the cheese and the film, enabling to offer the consumer a safer product.","palabras_clave":"NATAMYCIN | NISIN|EDIBLE FILMS|MIXED CULTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3650,"idioma_id":4,"titulo":"Present status of Accelerator-Based BNCT","resumen":"Aim: This work aims at giving an updated report of the worldwide status of Accelerator-Based BNCT (AB-BNCT). Background: There is a generalized perception that the availability of accelerators installed in hospitals, as neutron sources, may be crucial for the advancement of BNCT. Accordingly, in recent years a significant effort has started to develop such machines. Materials and methods: A variety of possible charged-particle induced nuclear reactions and the characteristics of the resulting neutron spectra are discussed along with the worldwide activity in suitable accelerator development. Results: Endothermic 7Li(p,n)7Be and 9Be(p,n)9B and exothermic 9Be(d,n)10B are compared. In addition to having much better thermo-mechanical properties than Li, Be as a target leads to stable products. This is a significant advantage for a hospital-based facility. 9Be(p,n)9B needs at least 4-5 MeV bombarding energy to have a sufficient yield, while 9Be(d,n)10B can be utilized at about 1.4 MeV, implying the smallest possible accelerator. This reaction operating with a thin target can produce a sufficiently soft spectrum to be viable for AB-BNCT. The machines considered are electrostatic single ended or tandem accelerators or radiofrequency quadrupoles plus drift tube Linacs. Conclusions: 7Li(p,n)7Be provides one of the best solutions for the production of epithermal neutron beams for deep-seated tumors. However, a Li-based target poses significant technological challenges. Hence, Be has been considered as an alternative target, both in combination with (p,n) and (d,n) reactions. 9Be(d,n)10B at 1.4 MeV, with a thin target has been shown to be a realistic option for the treatment of deep-seated lesions.","palabras_clave":"ACCELERATOR BASED BNCT|DIFFERENT NUCLEAR REACTIONS |ACCELERATOR TYPES|WORLDWIDE ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3651,"idioma_id":5,"titulo":"Organizaciones indígenas y Estado:  las demandas territoriales en contexto.","resumen":"En el primer informe oficial sobre conflictos rurales, elaborado por la Subsecretaría de Agricultura de la Nación en 2013, y publicado parcialmente por el periodista Darío Aranda en el Diario Página\/121 , se reconoció que existían casi diez millones de hectáreas pertenecientes a casi 65.000 familias campesinas e indígenas que eran pretendidas por empresarios privados o el propio Estado.Las superficies en conflicto relevadas en dicho informe equivalen a 455 veces el tamaño de la Ciudad Autónoma de Buenos Aires, representando un total de 857 casos, de los cuales 278 corresponden a distintas comunidades indígenas. La mayoría de ellos ?en el momento en que se llevó a cabo el relevamiento- tuvieron lugar en el norte del país y en la Patagonia (el NOA concentró la mayor cantidad de conflictos con el 28,2 por ciento, le sigue Patagonia con el 21,1 y el NEA con 19,8 por ciento del total).En relación con los conflictos territoriales que han sido relevados en el informe, uno de cada tres involucra a comunidades indígenas, y de éstos, sólo el cuarenta por ciento ha sido objeto de la ley Nº 26.160 de Relevamiento territorial. Esta norma, sancionada en noviembre del año 2006, acumula ya dos prórrogas (una en el año 2009, ley 26.554 y otra en 2013, la 26.894), dados los retrasos en su implementación y ejecución en algunas provincias.El escenario se presenta complejo y contradictorio. Por un lado, a nivel nacional, se verifica un proceso global de intensificación de la megaminería y los agronegocios que implica el corrimiento de la frontera agrícola y la concentración de la propiedad, y por otro lado, se ha producido un conjunto de normativas que impone el reconocimiento de derechos específicos a los pueblos originarios y la protección de sus territorios: desde la reforma constitucional de 1994, y la ley 24.071 de 1992 que implicó la aprobación del Convenio 169 de la OIT, hasta las regulaciones específicas aprobadas en los últimos años anteriormente citadas que declaran la emergencia territorial y prohíben los desalojos hasta tanto se finalice el relevamiento, pasando por otras como la ley 26.331 de Presupuestos Mínimos de Protección de los Bosques Nativos, sancionada en noviembre de 2007.Sin embargo, y a contramano de la abundancia de normativas ?protectoras? y ?reconocedoras?, el proceso mencionado de expansión de la frontera productiva pareciera conformar un límite sistemático a la vigencia de estas leyes.Podemos observar, por ejemplo, que en algunos medios los discursos racistas\/xenófobos en situaciones de conflicto entre el Estado (municipal, provincial o nacional) y las comunidades indígenas en Argentina, se constituyen en un elemento central del proyecto hegemónico que habilita diversas prácticas de inclusión\/exclusión, y han derivado en prácticas violentas y represivas en distintas regiones del país.En este sentido, el movimiento indígena desarrolla sus luchas en una relación dialéctica con la evolución de los distintos conflictos territoriales ? generados por el avance de los agronegocios y las distintas industrias extractivistas-, los agentes que intervienen (estatales o privados) y las interpretaciones de dichos conflictos que aparecen ante cada coyuntura.","palabras_clave":"TERRITORIOS|CONFLICTOS|INDÍGENAS|ESTADO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3652,"idioma_id":4,"titulo":"Clinical and epidemiological features of leishmaniasis in northwestern-Argentina through a retrospective analysis of recent cases","resumen":"Leishmaniasis is a parasitic disease caused by hemoflagellates of the genus Leishmania and is transmitted to humans by the bite of infected phlebotomine sandflies. Depending on the Leishmania species, the disease has different clinical forms including cutaneous, mucocutaneous, and visceral manifestations. Previous studies performed in endemic zones of northwestern-Argentina, during epidemic outbreaks, have been important for detecting patients suffering from the acute phase of the disease, but have not given a complete representation of the clinical and epidemiological features in the region. Furthermore, due to the resurgence of leishmaniasis worldwide and in particular the large increase of international tourism to the region, it seems pertinent to update the current epidemiological and clinical profile of leishmaniasis in northwestern-Argentina. Here we present a retrospective analysis of 95 Leishmania positive cases, presenting between 2000?2014. Patients were derived from hospitals and diagnosed in our lab at the University of Salta, located in a non-endemic area in Salta, Argentina. We detected numerous extensive mucocutaneous cases (34\/95, 35.8%) distinct from mucosal affected patients, some instances originating in locations with no previously reported human cases. Additionally patients suffering from concomitant diseases, besides leishmaniasis, were assessed. These included Chagas disease, syphilis, deep mycoses, tuberculosis, toxoplasmosis and intestinal parasitosis. This study updates the clinical and epidemiological features of leishmaniasis in northwestern-Argentina, and discusses the implications and management strategy for patients who acquire the disease in this region.","palabras_clave":"LEISHMANIASIS|PARASITIC DISEASES|CUTANEOUS LEISHMANIASIS|VISCERAL LEISHMANIASIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3653,"idioma_id":4,"titulo":"Biological relevance of Hsp90-binding immunophilins in cancer development and treatment","resumen":"Immunophilins are a family of intracellular receptors  for immunosuppressive drugs. Those immunophilins that are related to  immunosuppression are only the smallest proteins of the family, i.e. FKBP12 and  CyPA, whereas the other members of the family have higher molecular weight because  the show additional domains to the drug-binding site. Among these extra  domains, the TPR-domain is perhaps the most relevant because it permits the interaction  of high molecular weight immunophilins with the 90-kDa heat-shock protein,  Hsp90. This essential molecular chaperone regulates the biological function of  several protein-kinases, oncogenes, protein phosphatases, transcription factors  and cofactors. Hsp90-binding immunophilins where first characterized due to  their association with steroid receptors. They regulate the cytoplasmic  transport and the subcellular localization of these and other Hsp90 client  proteins, as well as transcriptional activity, cell proliferation, cell  differentiation and apoptosis. Hsp90-binding immunophilins are frequently  overexpressed in several types of cancers and play a key role in cell survival.  In this article we analyze the most important biological actions of the best  characterized Hsp90-binding immunophilins in both steroid receptor function and  cancer development, and discuss the potential use of these immunophilins with  therapeutic purposes as potential targets for specific small molecules.","palabras_clave":"FKBP38|FKBP51|FKBP52|FKBPL|NF-&#954;B|STEROID RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3654,"idioma_id":4,"titulo":"The electrocardiogram of anaesthetized southern sea lion (Otaria flavescens)  females","resumen":"Abstract Objectives: The goal of this study was to characterize for the first time theelectrocardiogram (ECG) of the southern sea lion (SSL) Otaria flavescens. Animals, materials and methods: Thirteen wild SSLfemales were captured at Isla de Lobos (Uruguay) and anesthetized with isoflurane. ECG recording was performed on anaesthetized animals atventral recumbence following standardized procedures. Results: The ECG recordingsshowed normal sinus rhythm. Amplitude and duration of P and T waves, QRScomplex, PR interval (PRi), QT interval (QTi) and ST segment (STs) were determinedfor all animals in all leads. QT corrected (QTc) was determined in lead II. Pwave polarity was consistent among animals (positive in LI, LII, LIII and AVFleads and negative in AVL and AVR leads for all animals), but T wave polaritydid not present any constant pattern among animals, being either positive, negativeor biphasic in different leads and different animals. The PRi (0.15 _ 0.2 s) wassimilar to the allometric prediction for most of mammalian species including humans.The STs were normal in 10 of the SSL but showed STs depression in 3 of theanimals. Almost all animals had a negative electrical axis (_30 to _120_), with one exceptionthat showed a positive electrical axis (120_). Mean eupneic heart rate (HR) was 104.61 _ 10.06 (range ¼ 88e120) beats per minute(bpm). Conclusions: This study was the first ECG description for this species, and provides valuableinformation for cardiac monitoring during anaesthesia. ","palabras_clave":"Electrocardiography|Pinnipeds|Isoflurane anaesthesia|Dive physiology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3655,"idioma_id":4,"titulo":"'Critical role for BRCA1 expression as a marker of chemosensitivity response and prognosis'","resumen":"Chemotherapy is still the leader option for cancer treatment. Nevertheless some patients develop chemotherapy resistance. One major research goal is to identify the critical genes involved in chemotherapy response to predict the best therapy option for patients. Germline mutations in the BReast Cancer susceptibility gene (BRCA1) are associated to increased risk of developing breast, ovarian and other types of cancers. However, due to harmful BRCA1 gene mutations are relatively rare in the general population, nowadays most researchers focused on BRCA1 expression downregulation and\/or epigenetic inactivation in sporadic tumors as a prognosis tool for chemotherapy response in patients. Chemotherapy response can be dramatically different depending on BRCA1 expression status, tumor type and drug. Hence, the chemotherapy response could be dissimilar in breast, ovarian, uterine, prostate, esophageal,  gastric and lung cancers. Additionally, differential BRCA1 expression in sporadic tumors shows different response to DNA-damaging agents, mitotic inhibitors or PARP inhibitors. In this review we will examine the response to different chemotherapy agents in several cancer types depending on BRCA1 expression status.","palabras_clave":"BRCA1|quimioterapia|cáncer|marcador pronóstico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3656,"idioma_id":4,"titulo":"A Norian Lagerpetid Dinosauromorph from the Quebrada del Barro Formation, Northwestern Argentina","resumen":"The early evolution of Ornithodira, the clade that includes pterosaurs and dinosaurs, is  poorly known. Until a decade ago, the basal radiation of Dinosauromorpha, the clade including  dinosaurs and birds, was poorly understood because of a scarce of fossil record, which was restricted  to specimens known of the Ladinian Chañares Formation from Argentina. In the last years the  discovery of several non-dinosaurian dinosauromorphs dramatically expanded this record and also demonstrated that this group?previously restricted to de Middle Triassic?persisted at least well  into the Norian. Although Norian non-dinosaurian dinosauromorphs have been reported from  several places around the world, the only known Norian non-dinosauriform dinosauromorphs? Dromomeron romeri and Dromomeron gregorii?come from North America. We report here the  first record from the Southern Hemisphere of a non-dinosauriform dinosauromorph, Dromomeron gigas sp. nov., from the Norian Quebrada del Barro Formation, northwestern Argentina. A  phylogenetic analysis recovers Dromomeron gigas nested into the monophyletic group  Lagerpetidae, and as the sister taxon to Dromomeron romeri. The inclusion of D. gigas within Lagerpetidae suggests that body size increased in this lineage over time, as was previously demonstrated for Dinosauriformes as a whole, and that lagerpetids reached a larger size than previously thought. Finally, the new finding provides novel information on the basal radiation of Dinosauromorpha constituting the first record of a Norian association of dinosaurs with non-dinosauriform dinosauromorphs outside North America.","palabras_clave":"Norian|Dinosauromorpha|Lagerpetidae|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3657,"idioma_id":5,"titulo":"Comer o ser comido: factores que determinan la condición nutricional de larvas de Engraulis anchoita de la población patagónica de la especie","resumen":"La población patagónica de Engraulis anchoita habita el sector costero comprendido entre los 42 y 46°S. Sus áreas de desove y cría están asociadas al frente de marea de Península Valdés, el cual se forma a fines de la primavera y desaparece a principios de otoño. Las condiciones oceanográficas del frente generan una alta productividad primaria y elevada biomasa del zooplancton asociado, potencial alimento para larvas de peces. Se estimó la condición nutricional de larvas de anchoíta empleando tres índices: el índice de Fulton, la relación ARN\/ADN y la performance de crecimiento. Se compararon dos secciones oceanográficas, una en la que se detectó la presencia del frente de marea y otra sin ese efecto. Los resultados de los índices mencionados evidenciaron una condición nutricional mejor en las larvas de anchoíta en la sección oceanográfica con persistencia del frente de marea. Con el objeto de determinar las variables ambientales que influencian la condición larval de E. anchoita se realizó un estudio aplicando Modelos Lineales Generalizados, considerando el índice ARN\/ADN como la variable dependiente y la clorofila superficial, la abundancia de nauplii, el volumen de plancton gelatinoso y el largo estándar de las larvas como variables explicativas. Se observó una mejora de la condición nutricional con el incremento de la abundancia de nauplii de copépodos, alimento potencial de las larvas. En cambio, el índice ARN\/ADN disminuyó con la abundancia de plancton gelatinoso, sugiriendo que estos organismos actuarían como competidores de las larvas de E. anchoita en la zona estudiada.","palabras_clave":"LARVAS DE ANCHOITA|INDICE ARN\/ADN|FRENTE DE MAREAS DE PENINSULA VALDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3658,"idioma_id":5,"titulo":"Esfera pública y redes sociales en Internet: ¿Qué es lo  nuevo en Facebook?","resumen":"H4 { margin-top: 0cm; margin-bottom: 0cm; direction: ltr; color: rgb(0, 0, 0); widows: 2; orphans: 2; }H4.western { font-family: 'Arial',sans-serif; font-weight: normal; }H4.cjk { font-family: 'Times New Roman',serif; font-weight: normal; }H4.ctl { font-family: 'Arial',sans-serif; font-size: 10pt; font-weight: normal; }P { margin-bottom: 0cm; direction: ltr; color: rgb(0, 0, 0); widows: 2; orphans: 2; }P.western { font-family: 'Arial',sans-serif; font-size: 12pt; font-weight: bold; }P.cjk { font-family: 'Times New Roman',serif; font-size: 12pt; font-weight: bold; }P.ctl { font-family: 'Arial',sans-serif; font-size: 10pt; }A:link { color: rgb(0, 0, 255); }Habitamos en sociedades altamentemediatizadas ya no sólo completamente atravesadas por la acción delos medios masivos de comunicación sino, también, por las nuevastensiones que asume el&nbsp;procesode mediatización&nbsp;apartir del desarrollo de las redes sociales en Internet. Al reconocerla naturaleza ambiental y constructiva de los medios masivos decomunicación es posible, también, considerar ala&nbsp;mediatización&nbsp;comomodalidad nuclear de construcción de la esfera pública. En dichocontexto, este escrito inquiere sobre los modos en que elfuncionamiento de Facebook y los discursos allí expuestos participanen la configuración de la esfera pública contemporánea, así como,además, sobre el grado de novedad que presenta dicha intervenciónrespecto de la ejercida por los tradicionales medios masivos decomunicación. En función de articular las reflexiones expuestas conalgunos de los ejes que ha asumido el debate teórico suscitado porel análisis que realizó Habermas sobre la génesis y lastransformaciones estructurales de la vida pública, este texto seconcentra en los siguientes tres aspectos: &nbsp;la gestión de lavisibilidad, de la puesta en público o publicación; el lugar queocupan el diálogo, la deliberación y el disenso; la condiciónmúltiple y móvil de la esfera pública actual.","palabras_clave":"mediatización|Facebook|Esfera pública|internet"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3659,"idioma_id":5,"titulo":"Relación entre estradiol plasmático, glucemia en ayunas y triglicéridos en varones con síndrome metabólico","resumen":"Introducción:En varones, el estradiol circula en pequeñas concentraciones (8 - 40 pg\/ml), sin estar del todo claras aún sus funciones. En este contexto, es necesario aclarar los efectos de esta hormona en el sexo masculino. Además de estudiarse el posible papel en la reproducción masculina, en los últimos años se ha tratado de encontrar la relación entre sus niveles y los componentes del síndrome metabólico. Sin embargo, la bibliografía existente no aclara si ejerce un papel protector o perjudicial con respecto a dicha patología.Objetivos: Analizar las relaciones existentes entre el estradiol y los componentes del síndrome metabólico en una muestra de varones excedidos de peso con síndrome metabólico de la provincia de Mendoza.Metodología: Se estudiaron 40 varones con sobrepeso u obesidad y síndrome metabólico residentes en la ciudad de Mendoza, con edades entre 53 y 74 años, los cuales fueron evaluados a partir de una consulta médica de rutina. Se determinó estradiol, testosterona, glucemia en ayunas, insulina en ayunas, índice HOMA, perfil lipídico y ácido úrico. Se evaluó el estado nutricional mediante antropometría: peso, talla, circunferencias, pliegues cutáneos y medidas derivadas. El análisis estadístico se realizó mediante correlación de Pearson o Spearman según la normalidad de las variables y prueba T de Student para muestras independientes. Se utilizó un nivel mínimo de significancia de p < 0,05.Resultados: Los niveles de estradiol se correlacionaron negativamente con los valores de triglicéridos (r= -0,401; p=0,013) y con la glucemia en ayunas (r= -0,333; p= 0,041). Por otra parte los individuos normotrigliceridémicos (valor de triglicéridos<150 mg\/dl) mostraron valores mayores de estradiol que los hipertrigliceridémicos (p=0,02).","palabras_clave":"ESTRÓGENOS|ENDOCRINOLOGÍA|SÍNDROME METABOLICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3660,"idioma_id":4,"titulo":"Biofertilization with Azospirillum brasilense improves in vitro culture of Handroanthus ochraceus, a forestry, ornamental and medicinal plant","resumen":"    Biofertilization with  plant growth-promoting rhizobacteria is a potential alternative to plant  productivity. Here, in vitro propagation of Handroanthus  ochraceus (yellow lapacho),  a forest crop with high economic and environmental value, was developed using  the Azospirillum brasilense strains Cd and Az39 during rhizogenesis.  Epicotiles of in vitro plantlets were  multiplied in Woody Plant Medium (WPM). For rooting, elongated shoots were  transferred to auxin-free Murashige?Skoog medium with Gamborg?s vitamins and  WPM, both at half salt concentration (½MSG and ½WPM), and inoculated with Cd or  Az39 at the base of each shoot. Anatomical  studies were performed using leaves cleared and stained with safranin for  optical microscopy and leaves and roots metalized with gold-palladium for  scanning electron microscopy (SEM). In  ½WPM auxin-free medium, A.  brasilense Cd inoculation produced 55% of rooting, increased root fresh and  dry weight (45% and 77%, respectively), and led to lower stomata size and  density with similar proportion of open and closed stomata.  Both strains selectively increased the size or density of glandular trichomes  in ½MSG. Moreover, bacteria were  detected on the root surface by SEM. In conclusion, the  difference in H. ochraceus response to A. brasilense  inoculation depends on the strain and the plant culture  media. Cd strain enhanced rooting in auxin-free ½WPM and produced plantlets with features  similar to those expected in ex vitro plants. This work presents  an innovative in vitro approach using  beneficial plant-microorganism interaction as an ecologically compatible  strategy in plant biotechnology.  ","palabras_clave":"AZOSPIRILLUM BRASILENSE |BIGNONIACEAE|MICROPROPAGATION|RHIZOBACTERIA|TREE PROPAGATION|YELLOW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3661,"idioma_id":4,"titulo":"The macrophytes Potamogeton pusillus L. and Myriophyllum aquaticum (Vell.) Verdc. as potential bioindicators of a river contaminated by heavy metals","resumen":"To evaluate the Myriophyllum aquaticum and Potamogeton pusillus macrophytes as indicator organisms of heavy metal pollution in biomonitoring studies of the aquatic ecosystem, the aim of this studywas to determine the Co,Cu, Fe, Mn, Ni, Pb and Zn accumulation in leaves of the those species and the possible relationship to water pollution by thesemetals. Surfacewater, sediment and plantswere collected at 10 sampling sites of the Ctalamochitariver (Argentina). Cooper and Pb concentrations exceeded the limits established for the protection of aquatic life defined by Argentina Legislation (Cu: 2.0 &#956;gL&#8722;1, Pb: 2.0 &#956;gL&#8722;1) and international norms (Cu: 1.6 &#956;gL&#8722;1, Pb: 2.5 &#956;gL&#8722;1) in surface water, while Cu and Zn exceeded the limit for ecological screening levels (Cu: 31.6 mgkg&#8722;1, Zn: 121.0 mgkg&#8722;1) in sediment. Heavy metal concentrationswere found to be higher downstream of Río Tercero city in water and sediments samples, probably related to the contribution of pollutants from the effluent discharge of the city. Both species revealed a high capacity to accumulate heavy metals in its tissues, in areas of the river with higher heavy metals values in the abiotic compartments. Particularly, high accumulation of Co, Cu, Ni and Zn in P. pusillus correlatedwith their concentrations in sediments and Co, Cu, Mnand Zn accumulation in M. aquaticum correlated with the concentrations of these metals in water. These macrophytes reflect spatialvariations of metals in water and sediments of the Ctalamochita river; therefore they are of potential use as heavy metal bioindicators of river pollution.","palabras_clave":"bioaccumulation|aquatic plants|pollution|heavy metals|river"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3662,"idioma_id":4,"titulo":"Annual plant functional traits explain shrub facilitation in a desert community","resumen":"AbstractQuestion: For a desert where winter is the driest, harshest season we asked: does the effect of dominant shrubs (Bulnesia retama) on annual species depend on (1) the functional traits of the latter, (2) the season of the year, or (3) the activity of livestock?Location: A low-density goat farm in central-northern Monte Desert, Argen- tina.Methods:We estimated the effect of shrubs using a log response ratio based on annual species population sizes underneath shrub canopies and in open spaces. We collected density data of annual species in 18 visits between Aug 2010 and Apr 2013 in permanent 50-cm square areas laid out according to a split-plot design, inwhich the activity of livestock (fenced and unfenced; plots were 10-m squares)was themain factor, and microsite type (shrub and open) was the sub- ordinate factor, with 20 replicates for each combination. We also gathered data on eight functional traits (characterizing whole plants, leaves, roots and seeds) fromannual plants collected in the study site following standardized protocols.Results: Annual species with acquisitive attributes (high specific leaf area, inter- mediate-to-low leaf dry matter content, large leaves and high specific root vol- ume) were more benefited by shrubs compared to species with the opposite, relatively conservative attributes. Facilitative influences of shrubs were perva- sive during winter, while competitive influences increased in frequency during summer, when total plant density was higher. This pattern was not affected by livestock.Conclusions: The outcome of species interactions depended on the interplay between plant strategies and abiotic stress: facilitated speciesweremostly acquis- itive, and shrub facilitation was more important during the harshest season (winter). Specific root volume, alongwithwidely used functional traits (specific leaf area, leaf dry matter content, leaf size) delineated such strategies. This underlines the importance of considering below-ground traits when studying plant?plant interactions. Single traits achieved superior explanatory power of shrub effects than composite ones (i.e. principal component axes based on single traits), reinforcing the idea that single functional traits are themselvesmeaning- ful indicators of complex physiological trade-offs that ultimately affect commu- nity structure and dynamics.","palabras_clave":"ABIOTIC STRESS|ARID ENVIRONMENT|COMPETITION|DRYLAND|FACILITATION|MONTE DESERT|NURSE PLANT|PLANT STRATEGIES|PLANTPLANT INTERACTION|SPECIFIC ROOT VOLUME|STRESS-GRADIENT HYPOTHESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3663,"idioma_id":4,"titulo":"HEPATIC PRENEOPLASIA INDUCTION IN MALE WISTAR RATS: HISTOLOGICAL STUDIES UP TO FIVE MONTHS POST TREATMENT","resumen":"Background: Liver preneoplasia development in rats canbe mimicked by an initiation-promotion model that induces theappearance of altered hepatocyte foci (FAH).Aims: We compare two initiation-promotion models to evaluatethe presence of FAH or additional hepatic pathologies in whichother organs were affected up to five month post treatment.Material and methods: FAH were induced in maleadult Wistar rats with two doses of dietylnitrosamine (DEN,150 mg\/kg bw) followed by 4 doses per week (3 weeks) of2-acetylaminofluorene (2-AAF, 20 mg\/kg bw) or with one doseof DEN (200 mg\/kg bw) followed by 2 doses per week (3 weeks)of 2-AAF. DEN 150, DEN 200 and control rats (received thevehicle of the drugs) groups were compared. Rats were euthanizedimmediately after the last dose of 2-AAF, at 3, 4 and 5 months(n = 3 for euthanasia times per group). Samples of livers, lungs,kidneys, pancreatic tissue and small bowel were processed forhistological and immunohistochemical analysis.Results: FAH persisted for 5 months in all livers of the DENgroups. Three months after withdrawal of 2-AAF, one rat fromDEN 150 group developed fibrosis and 5 months after 2-AAFremoval another rat from the same group presented a microscopichyperplastic nodule. Only the lungs had damages compatible withlesions induced by gavage-related reflux in DEN groups.Conclusion: We concluded that up to five month posttreatments, FAH persisted in all the livers from DEN groups; liversfrom DEN 200 group showed no other hepatic lesions besidesFAH, and only the lungs suffered pathological alterations in both treated groups.","palabras_clave":"diethylnotrosamine|2-acetylaminofluorene|rat liver preneoplasia|altered hepatocyte foci;|reflux"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3664,"idioma_id":5,"titulo":"Análisis panbiogeográfico del Genero Galianthe Subgénero Ebelia (Rubiaceae)","resumen":"Galianthe (Rubiaceae: Spermacoceae) es un género con los subgéneros Galianthe y Ebelia, éste último comprende 11 especies neotropicales, con centro de diversidad se encuentra en Brasil. El análisis panbiogeográfíco enfatiza la importancia de la dimensión espacial de la biodiversidad para generar una comprensión más adecuada de los patrones y procesos evolutivos y enfoca los eventos vicariantes como la mayor fuerza de la fragmentación de biotas. Son escasos los estudios biogeográficos de Galianthe, por lo que el objetivo del presente trabajo es establecer los patrones de distribución de Ebelia mediante un análisis de trazos. Las especies poseen distribución mesoamericana, andina y chaqueña, cuatro de estas últimas son exclusivas de Brasil. Siete especies conforman un único trazo generalizado denominado ?Paraná?, que conecta las provincias biogeográficas Cerrado y Chaco del dominio Chaqueño y Atlántica, Bosque Paraná y Bosque de Araucaria, pertenecientes al dominio Paraná, ambos dominios se hallan íntegramente dentro de la subregión Chaqueña, región Neotropical. Debido a que el origen de la tribu Spermacoceae se ha establecido durante el Eoceno, la aridificación de Sudamérica generada por los eventos vicariantes como la orogenia andina e introgresiones marinas, posiblemente han favorecido la radiación adaptativa del subgénero Ebelia dentro de la subregión Chaqueña.","palabras_clave":"PANBIOGEOGRAFÍA|REGIÓN NEOROPICAL|SPERMACOCEAE|SUBREGIÓN CHAQUEÑA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3665,"idioma_id":4,"titulo":"Carbon footprint of honey produced in Argentina","resumen":"The urgent need to mitigate the emissions of gases that contribute to climate change is creating a new scenario for the international trade of goods and services. Countries that are making efforts to reduce those emissions are now demanding that imported goods contain low levels of embedded carbon for those goods to fairly compete with locally produced products.  For this purpose the implementation of standards such as the ?carbon footprint? becomes critical. Carbon footprint is an environmental sustainability indicator that quantifies the emissions of greenhouse gases generated during the lifecycle of a product. Argentina, as an exporter of agricultural products, has conducted initial studies to estimate the carbon footprint of several food products of high impact in its exports such as meat and wine but yet little had been done about honey production even though the country is one of the world´s three largest exporters. This study is the first assessment of the carbon footprint of honey produced in Argentina where almost 95% of the production goes to the export markets. This study also adds scientific value by comparing economic value-based allocation and production-based allocation. Official information, interviews and surveys to key stakeholders in the Argentinean beekeeping chain revealed that the cluster is characterized by low technology development and small-scale production based exclusively in the sale of a commodity (bulk product without differentiation) but recognized by its genuine quality valued on flavor and safety issues. Carbon footprint of honey evaluates greenhouse gas emissions throughout the lifecycle; specifically carbon dioxide, nitrous oxide and methane. Activity data collected includes annual honey production, geographical location of beehives and processing plants, technology used, and fuels and energy consumption. Based on the ISO 14040 method that uses lifecycle assessment, the calculated carbon footprint of honey is 2.5 ± 0.17 kg CO2e\/kg honey, being the extraction process responsible for the highest contribution to GHG emissions (90.7%) in contrast to hive management and freight emissions. A strong linear, positive correlation (R2=0.999) is found between process emissions and honey production pointing out a high dependence of the process with the use of fossil energy. Carbon dioxide is the dominant GHG emitted (98%) compared to methane and nitrous oxide emissions. The subdivision method applied to calculate emissions allows tracing the burden of each phase of the production process; the functional unit used to estimate emissions proved adequate for comparison purposes and the results achieved were close to those reported in the literature in spite of honey carbon footprint is strongly dependent on the production practices and honey beekeeping chain characteristics. From the results obtained, energy efficiency measures during the processing stage could alleviate the carbon impact of honey production in Argentina.","palabras_clave":"CARBON FOOTPRINT|HONEY|GHG EMISSIONS|ARGENTINEAN BEEKEEPING CHAIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3666,"idioma_id":4,"titulo":"Differential transcriptional responses underlie dietary induction of intestinal carbohydrase activities in house sparrow nestlings","resumen":"Many species show diet-induced flexibility of activity of intestinal enzymes; however, molecular and genetic mechanisms responsible for such modulation are less known, particularly in altricial birds. The goal of our study was to test whether a diet-induced increase of activity of intestinal maltase and sucrase in house sparrow nestlings is matched with an increase in maltase-glucoamylase (MG) and sucrase-isomaltase (SI) complex mRNAs, respectively.  Both enzyme activities were significantly higher in mid intestine of nestlings fed a medium starch (MS) diet compared to those fed a starch-free (SF) diet. In contrast to the similar pattern of dietary induction for both enzyme activities, diet MS elevated significantly only the level of MG mRNA, but not SI mRNA.  The coordinated increase in activity of maltase and in MG mRNA is consistent with the hypothesis that dietary induction of this enzyme is under transcriptional control. In contrast, the lack of such coordination for changes in activity of sucrase and SI mRNA suggests that up-regulation of this enzyme may be achieved by post-translational factor(s). We conclude that genetic mechanisms responsible for diet-induced flexibility of digestive enzymes in birds may differ from that observed in mammals.","palabras_clave":"maltase-glucoamylase|sucrase-isomaltase|enzyme activity|gene expression|small intestine|house sparrow|nestlings"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3667,"idioma_id":5,"titulo":"Procesos de clusterización: la territorialidad de la producción quesera en el sudeste pampeano","resumen":"Resumen El territorio modificado constantemente por redes está direccionado por la multiplicación de flujos entre nodos que rigen la economía y la sociedad a escala local-global. Las transformaciones derivadas no son coyunturales, sino estructurales, originadas desde el régimen de acumulación fordista hacia una nueva lógica del capitalismo actual identificado como posfordista. El objetivo de este artículo es explicar el proceso de construcción del clúster quesero Tandil (CQT) focalizando el análisis en el proceso de articulación multiescalar de actores y su rol en la construcción territorial local-regional. El estudio de caso fue realizado a partir de los '... desafíos metodológicos para las investigaciones empíricas' propuestos por Fernández y Vigil (2007: 885). De acuerdo con ello, el CQT se compone de 28 empresas que procesan anualmente 35,6 millones de litros, de los cuales el 85 % se destina a la elaboración de 3.300 tn de queso\/año, dinamizan 189 empleos directos y facturan estimativamente entre 100 y 115 millones de pesos en el año 2012. Las evidencias empíricas ponen de relieve que el Estado cumple un rol determinante para crear y estimular acciones de desarrollo territorial. Las redes productivas e institucionales se expresan multiescalarmente, jerarquizadas y asimétricas, con diferente representatividad según los momentos\/contextos. A partir de lo enunciado, nuestro interés se centra en los interrogantes: ¿estamos ante una aglomeración productiva homogénea, autónoma, que asegura el crecimiento y la cooperación? La respuesta podría ayudarnos a responder nuestra pregunta inicial: Clúster quesero. ¿herramienta de desarrollo territorial o de posicionamiento productivo?","palabras_clave":"CLUSTER QUESERO|ACTORES|TERRITORIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3668,"idioma_id":5,"titulo":"Desempeño del método de análisis transitorio en la detección de fallas paramétricas en circuitos integrados","resumen":"En este trabajo seevalúa la capacidad de la estrategia de test denominada Metodología de Análisisde Respuesta Transitoria (TRAM, por sus siglas en inglés) para discriminarentre circuitos dentro y fuera de especificaciones. Con este propósito seadopta una vista a nivel comportamiento, definiendo una falla como unincumplimiento de alguna de las especificaciones. Si bien esta estrategia detest ha sido estudiada por varios autores, este trabajo se enfoca en lautilización de modelos de simulación más precisos que evitan hacer suposicionesde comportamientos ideales. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Para las evaluacionesse ha adoptado un filtro de segundo orden en la topología de variables deestados, el cual ha sido diseñado totalmente a medida (full custom) en una tecnología comercial CMOS de 500nm.Se adoptauna metodología de evaluación que inyecta desviaciones aleatorias en losparámetros circuitales como un medio para generar una población de circuitoscon diferentes proporciones de buenos y malos. Esta población se exponeposteriormente al test para evaluar su eficiencia.","palabras_clave":"Circuitos Integrados Analógicos|Detección de Fallas|TRAM|Respuesta Transitoria|CMOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3669,"idioma_id":5,"titulo":"'Reeducar inválidos es un problema caro'. La rehabilitación laboral y la reinserción social. Argentina, 1915- 1960","resumen":"En este artículo se examinan lasideas, las políticas y las prácticas efectivas que intentaron, con resultadosmuy dispares, resolver las demandas de rehabilitación laboral y reinserciónsocial en la Argentina, entre 1915 y1960. A partir de los relatos oficiales de los tres primeros congresos médicos sobre la problemáticadel ?lisiado? realizados en 1943, 1946 y 1959, publicaciones científicas, debatesde normativas y leyes emanadas del Poder Ejecutivo y Legislativo plantean interrogantes sobre cómo seconformaron las relaciones entre el mundo laboral y las personas condiscapacidad y, en particular, cómo se entretejieron las prácticas médicas derehabilitación y el acceso al trabajo con las discusiones sobre quiénes y cómose solventarían las medidas de protección a la invalidez. En las conclusionesse indica que, en nuestro país a lo largo del siglo XX, lo que primó fueuna gran distancia entre los discursos, las intenciones y las concreciones,develando que para las personas con discapacidades el acceso al trabajo comoelemento integrador de ciudadanía social y como derecho es aún una deuda a ser saldada.","palabras_clave":"REHABILITACIÓN LABORAL|REINSERCiÓN SOCIAL|DISCAPACIDAD|POLÏTICAS SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3670,"idioma_id":4,"titulo":"Factors affecting the spatial ecology of the lizard Liolaemus wiegmannii in the pampasic coastal dunes of Argentina","resumen":"Home ranges of lizards are the result of both internal (body condition, reproductive status) and external&nbsp; factors, such as habitat features and resource availability. Habitat modification induced by introduced&nbsp; plants affects habitat use for lizards by changing food abundance, environmental temperatures or by&nbsp; homogenizing the habitat structure. We compared the home range of the lizard Liolaemus wiegmannii&nbsp; in two situations: a partially forested habitat (20% of the total surface covered by Acacia longifolia) and&nbsp; a non-forested habitat. Twelve adult lizards were radio-tracked in the forested habitat and ten in the&nbsp; non-forested site. Home ranges were calculated using the minimum convex polygon method. The mean&nbsp; home range size was 37.80±17.95 m2 and was not different between both habitat types. Male?s home&nbsp; ranges were 1.6 times larger than those of females. Abundance of food was highest in the forested&nbsp; habitat, nonetheless, its effect on home range size was not evident. Home range in L. wiegmannii&nbsp; showed a marked association with mixed patches of native grassland, bare sand substrates and scarce&nbsp; coverage of exotic trees. Our data suggest that movements in L. wiegmannii may be mainly related to&nbsp; structural features (and their associated thermal cues) of specific microhabitats types. Although low&nbsp; levels of forestation with A. longifolia have less effect on the home range size and movements of&nbsp; lizards, we cannot ignore previous results showing that occurrence, abundance, and body condition of&nbsp; L. wiegmannii are negatively affected by extensive forestation of exotic plants both at local and&nbsp; landscape scales in pampasic dunes.","palabras_clave":"Liolaemus|sand-lizard|radio-telemetry|home range"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3671,"idioma_id":4,"titulo":"Feeding regime and food availability determine behavioural decisions under predation risk in Pleurodema thaul (Anura: Leiuperidae) tadpoles","resumen":"Movement makes prey more vulnerable to predators. Antipredator responses usuallyhave associated costs such as reduced feeding activity, and decisions made underpredation risk may be affected by the feeding state of the prey. In this study, we evaluated whether food regime influences antipredator behavioural responses of Pleurodema thaul tadpoles (diet treatment) before adding food or water (incentive treatment) while the tadpoles were exposed to alarm cues. Under predation risk, normally fed tadpoles showed decreased overall activity, whereas poorly fed tadpoles spent more time feeding after food was provided. Tadpoles in all treatments decreased their swimming activity but not their feeding activity. Our study showed that swimming behaviour was more sensitive to predation risk than feeding behaviour. We suggest that the motivational state of tadpoles under different environmental conditions should be considered when behavioural patterns are analyzed to understand short-term trade-offs between foraging requirements and predator avoidance.","palabras_clave":"predation risk|diet|tadpoles|behaviour"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3672,"idioma_id":5,"titulo":"La incidencia de la tesis de la neutralidad valorativa en la enseñanza de las ciencias económicas. Ética, economía y educación: por una enseñanza pluralista de las ciencias económicas","resumen":"Tomando como punto de partida los recientes cuestionamientos que desde distintos movimientos de grupos de economistas y estudiantes de economía vienen realizando a la ortodoxia de la enseñanza de las ciencias económicas, en este estudio pretendemos, en primer lugar, evaluar la incidencia de la tesis de la neutralidad valorativa de las ciencias en la enseñanza de la economía, sosteniendo que la tan mentada neutralidad valorativa no es más que un mito, puesto que es ella misma un valor, y en segundo lugar, frente a esto, señalar la necesidad del disenso y de una enseñanza más pluralista que posibilite la construcción de un verdadero estado democrático.","palabras_clave":"NEUTRALIDAD VALORATIVA|ECONOMIA|POLITICA|PLURALISMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3673,"idioma_id":5,"titulo":"Aplicación de análisis de imágenes digitales a la deshidratación de rodajas de anco (Cucurbita moschata)","resumen":"Se trabajó con imágenes digitales de rodajas de Cucurbita moschata que fueron sometidas a deshidratación en un secador solar. El análisis realizado sobre las imágenes digitales permitió obtener resultados cuantitativos sobre el fenómeno de encogimiento tanto en área como en perímetro. Este análisis se contrastó con los resultados experimentales obtenidos con mediciones convencionales de humedad de las muestras y valores reportados en la bibliografía. Se observó que los resultados obtenidos muestran buena concordancia con las curvas experimentales, corroborando que la integración de sistemas de visión por computador es una herramienta eficaz en la evaluación de calidad de productos deshidratados","palabras_clave":"imagen digital|visión por computadora|secadero solar"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3674,"idioma_id":4,"titulo":"Occurrence and distribution of arsenic in the sediments of a carbonate-rich unsaturated zone","resumen":"Three combined approaches were used to study the arsenic (As) occurrence in carbonate-rich unsaturated zone from a large plain environment (Pampean plain, Argentina). A sequential extraction procedure (SEP) was used to identify the As-bearing phases and distribution along a 2-m-long unsaturated zone profile, whereas XRD and SEM\/EDS were used to determine the mineralogical features of the sediments. In this hydrogeologic environment, groundwater levels tend to be close to the surface (&lt;5 m) and hydrogeochemical processes that develop during infiltration and recharge through the unsaturated zone may release the As hosted in the sediments to shallow aquifers. According to SEP results, the unsaturated zone was divided in an upper and a lower zones. Disregarding the As contained in the silicates, low crystalline Fe(III) oxyhydroxides were the main As-bearing minerals in the upper zone. Its presence was confirmed by SEM\/EDS analysis. In the lower zone, carbonates were the main As-bearing minerals, also supported by XRD analysis. The chemical composition from the resulting water-extractable step of the SEP was constrained using geochemical inverse modeling (PHREEQC) to elucidate thosepotential geochemical processes that may control the As release or retention during, for instance, an infiltration event. They showed that Na\/Ca exchange promoted the calcitedissolution which, in turn, raised the pH as a result of proton consumption. High pH and HCO3- contents favored the As desorption from low crystalline Fe(III) oxyhydroxides in the upper zone, where they were present in higher proportion.","palabras_clave":"unsaturated zone|arsenic|sequential extraction procedure|inverse modeling|Na\/Ca Exchange|Carbonate-rich sediments"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3675,"idioma_id":4,"titulo":"Highly doped ZnO films deposited by spray-pyrolysis. Design parameters for optoelectronic applications","resumen":"Synthesis and preparation of ZnO films are relevant subjects for obtaining transparent and conducting layers with interesting applications in optoelectronics and photovoltaics. Optimization of parameters such as dopant type and concentration, deposition time and substrate temperature is important for obtaining ZnO layers with optimal properties. In this work we present a study about the induced effects of deposition time on optical and electrical properties of ZnO thin films. These films were deposited by spray pyrolysis of a suitable Zn precursor, obtained through the sol?gel method. The deposition time has direct incidence on internal stress in the crystal structure, generating defects thatmay affect transparency and electrical transport into the layers. We performed mosaicity measurements, through X-ray diffraction, and used it as a tool to get an insight on structural characteristics and homogeneity of ZnO layers. Also, through this technique,we analyzed thickness and doping effects on crystallinity and carrier transport properties.","palabras_clave":"SPRAY PYROLYSIS|SOL-GEL|ZINC OXIDE|HETEROJUNCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3676,"idioma_id":4,"titulo":"Human impacts and aridity differentially alter soil N availability in drylands worldwide","resumen":"AimsClimate and human impacts are changing the nitrogen (N) inputs and losses in terrestrial ecosystems. However, it is largely unknown how these two major drivers of global change will simultaneously influence the N cycle in drylands, the largest terrestrial biome on the planet. We conducted a global observational study to evaluate how aridity and human impacts, together with biotic and abiotic factors, affect key soil variables of the N cycle.LocationTwo hundred and twenty-four dryland sites from all continents except Antarctica widely differing in their environmental conditions and human influence.MethodsUsing a standardized field survey, we measured aridity, human impacts (i.e. proxies of land uses and air pollution), key biophysical variables (i.e. soil pH and texture and total plant cover) and six important variables related to N cycling in soils: total N, organic N, ammonium, nitrate, dissolved organic:inorganic N and N mineralization rates. We used structural equation modelling to assess the direct and indirect effects of aridity, human impacts and key biophysical variables on the N cycle.ResultsHuman impacts increased the concentration of total N, while aridity reduced it. The effects of aridity and human impacts on the N cycle were spatially disconnected, which may favour scarcity of N in the most arid areas and promote its accumulation in the least arid areas.Main conclusionsWe found that increasing aridity and anthropogenic pressure are spatially disconnected in drylands. This implies that while places with low aridity and high human impact accumulate N, most arid sites with the lowest human impacts lose N. Our analyses also provide evidence that both increasing aridity and human impacts may enhance the relative dominance of inorganic N in dryland soils, having a negative impact on key functions and services provided by these ecosystems.","palabras_clave":"Aridity, , , , , N cycle.|depolymerization|global change|human impacts|mineralization|N cycle"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3677,"idioma_id":4,"titulo":"First report of orange rust of sugarcane caused by Puccinia kuehnii in Argentina","resumen":"In April 2015, lesions typical of orange rust were observed in a commercial sugarcane field in Fachinal, Misiones Province, Argentina, on a nonidentified cultivar (a complex hybrid of Saccharum spp.). Symptoms on leaves differed from those of brown rust of sugarcane (Puccinia melanocephala), commonly observed in the region. Leaf samples were analyzed both at the EEAOC and INTA Plant Pathology laboratories in Tucumán, Argentina. Light microscope studies revealed that uredinia and urediniospore were similar morphologically to those described for the fungus Puccinia kuehnii E. J. Butler, causal agent of sugarcane orange rust (Virtudazo et al. 2001a). Uredinial lesions were orange and variable in size, hypophyllous, ellipsoidal to fusiform in shape, and distinctly lighter than pustules of P. melanocephala that were present along with P. kuehnii. Urediniospores were mostly obovoid to pyriform, variable in size (29 to 41 × 19 to 26 &#956;m), and moderately echinulate with mostly evenly distributed spines 2 to 4.5 &#956;m apart. Walls were orange-to-light cinnamon brown with a pronounced apical wall thickening as much as 7 &#956;m. Paraphyses, telia, and teliospores were not observed. Identification of the pathogen was confirmed by molecular studies. The internal transcribed spacer region (ITS1-5.8S-ITS2) of the rust infecting a nonidentified sugarcane cultivar (GenBank Accession No. KT261382) was sequenced. The sequence was identical to sequences of P. kuehnii and distinct from known sequences of P. melanocephala (Virtudazo et al. 2001b). Orange rust, initially reported in the Asia-Pacific region, has caused important losses in Australia in 2000. In the Western Hemisphere, it was first reported in Florida in 2007 (Comstock et al. 2008). Since being reported in Brazil in 2009 (Barbasso et al. 2010), annual surveys have been conducted in all three sugarcane production regions of Argentina: Northern (Salta and Jujuy provinces); Northeast (Santa Fe and Misiones provinces); and Tucumán Province. At present, the only focus of orange rust infection detected is the one in Misiones Province, 1,200 km from Tucumán, the main sugarcane-growing region of the country. To our knowledge, this is the first report of P. kuehnii infecting sugarcane in Argentina. The possible economic impact of orange rust on sugarcane production in the country is still unknown, but considering its destructive potential, the disease may pose a serious threat to sugarcane production if environmental conditions favor its establishment during the early growth stages of the crop.","palabras_clave":"MISIONES PROVINCE|UREDINIA|UREDINIOSPORE|ITS1-5.8S-ITS2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3678,"idioma_id":4,"titulo":"Contribution of Amynthas gracilis (Megascolecidae) and Octolasion cyaneum (Lumbricidae) to soil physical stability: A mesocosm experiment","resumen":"The contribution of the introduced species Amynthas gracilis (Kinberg, 1867) and Octolasion cyaneum  (Savigny, 1826)  to the physical stability of the soil was evaluated in a mesocosm experiment.  Pore formation and stable aggregates were measured; as well as changes in bulk density, porosity, and soil moisture. Mesocosm pots were organized into three treatments: 1- soil + Amynthas gracilis, 2- soil + Octolasion cyaneum and 3- soil (control containers). The experiment ran for 13 weeks and it was conducted in controlled conditions in a greenhouse. At the end of the experiment both treatments with earthworms had higher number of pores and stable aggregates at the two considered depths (0 - 5 cm and 5 - 10 cm). The presence of both earthworm species favors the formation of a  significantly higher proportion of stable aggregates larger than 5 mm (60%), when compared to the control without worms. These structures helped maintaining bulk density and porosity and improved water circulation. The results show that when compared to the control, both treatments had a lower loss of pore space, lower bulk density, and higher soil moisture, all attributable to earthworm presence. It is concluded that, despite both being introduced species, in intensive agricultural systems, A. gracilis  and O. cyaneum can contribute to the maintenance of soil physical stability thus helping to preserve the sustainability of agro-ecosystems, even if native species became rare or locally extinct.","palabras_clave":"pores|soil physical stability|aggregate stability| introduced species|earthworms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3679,"idioma_id":5,"titulo":"'La manifestación territorial de las desigualdades  socioeconómicas en Argentina: Del diagnóstico a las políticas públicas',","resumen":"En el trabajo analizamos las brechas socioeconómicas entre provincias argentinas en los años 2000 (encontrando un aumento en la mayoría de las dimensiones) y la distribución de funciones y recursos entre jurisdicciones del Estado (las capacidades tributarias provinciales fueron insuficientes y la distribución de los fondos nacionales consolidó asimetrías preexistentes). Lograr una intervención redistributiva territorialmente requiere aumentar los recursos subnacionales, compensar diferentes capacidades tributarias, pensar políticas coherentes, asignar fondos nacionales progresivamente, favorecer círculos virtuosos de desarrollo y generar capacidades locales y oportunidades de empleo.","palabras_clave":"DESIGUALDADES TERRITORIALES|FINANZAS PUBLICAS|RELACIÓN NACION-PROVINCIAS-MUNICIPIOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3680,"idioma_id":4,"titulo":"Adsorption and mobility of glyphosate in different soils under no-till and conventional tillage","resumen":"Glyphosate (N-(phosphonomethyl) glycine) is a post-emergence, non-selective, foliar herbicide. Around 200 million liters of this herbicide are applied every year in Argentina, where the main agricultural practice is no-till (NT), accounting for 78.5% of the cultivated land. In this work, we studied the adsorption of glyphosate in different soils under long-term management (more than 16 years) of NT and conventional tillage (CT). Samples were taken from different regions of Argentina corresponding to: Paraná soil (PAR), a silty clay loam soil (< 37% clay), Manfredi (MAN) and Pergamino (PER), both silty loam soils (< 26% clay). We found that the adsorption was very high in all the soils, and it was particularly influenced by the soil clay content and CEC and negatively related to pH and phosphorus. In general, the adsorption coefficient (Kf) was higher in the CT samples. We also studied the vertical transport of glyphosate in undisturbed columns (15 cm long) and compared the effect of NT and CT. Less than 0.24% of the applied pesticide leached in all soils. No significant difference was found between the total amount of leached glyphosate between soils or tillage practice. The highest glyphosate concentration (67.53% of the initially applied doses) was found in the top 5 cm of the columns. The strong retention of glyphosate to the soil matrix, as confirmed by the high Kf values obtained in the isotherm studies, was the dominant factor influencing glyphosate mobility through the soil profile.","palabras_clave":"Glyphosate; |Adsorption; | Leaching; |No-till;|Conventional tillage"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3681,"idioma_id":4,"titulo":"Biomarkers of oxidative damage and antioxidant defense capacity in Caiman latirostris blood","resumen":"Several xenobiotics, and among them pesticides, can produce oxidative stress, providing a mechanistic basis for their observed toxicity. Chronic oxidative stress induces deleterious modifications to DNA, lipids and proteins that are used as effective biomarkers to study pollutant-mediated oxidative stress. No previous report existed on the application of oxidative damage and antioxidant defense biomarkers in Caiman latirostris blood, while few studies reported in other crocodilians were done in organs or muscles of dead animals. The aim of this study was to characterize a new set of oxidative stress biomarkers in C. latirostris blood, through the modification of conventional techniques: 1) damage to lipids by Thiobarbituric Acid Reactive Substances (TBARS), 2) damage to DNA by Comet assay modified with the enzymes FPG and Endo III, 3) antioxidant defenses: Catalase, Superoxide dismutase and Glutation; in order to apply them in future biomonitoring studies. We successfully adapted standard procedures for CAT, SOD, GSH and TBARS determination in C. latirostris blood. Calibration curves for FPG and Endo III showed that the three dilutions tested were appropriate to conduct the modified comet assay for detection of oxidized bases in C. latirostris erythrocytes. One hour of incubation allowed a complete repair of the damage generated. The incorporation of these biomarkers in biomonitoring studies of caiman populations exposed to xenobiotics is highly important considering that this species has recovered from a serious endangered state through the implementation of sustainable use programs in Argentina, and represents nowadays a relevant economic resource for many human communities","palabras_clave":"CROCODILES|OXIDATIVE STRESS|BIOMARKERS|XENOBIOTICS|REPTILES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3682,"idioma_id":4,"titulo":"Strain dependence of the physical properties of epitaxial La0.7Ca0.3MnO3 thin films grown on LaAlO3 substrates","resumen":"We present a systematic study of the structural, magnetic, electrical and magnetoresistive properties of La0.7Ca0.3MnO3 thin films epitaxially grown on LaAlO3 single crystalline substrates using metal organic deposition process. The evolutions of the lattice parameters and the corresponding strain as a function of the film thickness (20?80 nm) have been investigated using X-ray diffraction measurements. The films were found to be totally relaxed for a thickness around 60 nm. Magnetization and resistance measurements as a function of temperature revealed a direct correlation of the transition temperature from a ferromagnetic state to the paramagnetic state with the film thickness. The temperature dependence of the resistivity (&#961; (T)) has been fitted using various theoretical approaches. Below the transition temperature (TP) the &#961; (T) graphs were well fitted using the &#961;(T) = &#961;0 + AT&#945; formula, in which the fitting parameters &#961;0 and &#945; have been used to clarify the conduction mechanism. Above TP, the &#961; (T) graphs were found to be well fitted using different models including the VRH model and the small polaron model. A magnetoresistance of 91% was measured at 248 K for the for 60 nm thick film under an applied magnetic field of 7 T. As well as a non-volatile resistive switching capacity of 15% on Ag contacts deposited on top of this film.","palabras_clave":"Thin films|Manganites|Transport properties|Resistive switching"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3683,"idioma_id":4,"titulo":"Redescription and life cycle of the monorchiid Postmonorcheides maclovini (Szidat, 1950) (Digenea) from the Southwestern Atlantic Ocean: morphological and molecular data","resumen":"The adult monorchiid, Postmonorcheides maclovini Szidat, 1950, digenean parasite of the Patagonian blennie Eleginops maclovinus (Cuvier) (Eleginopidae) from Puerto Deseado (47° 45&#8242; S, 65° 55&#8242; W), Argentina, was characterized and its life cycle elucidated. P. maclovinus is the only species of the genus Postmonorcheides, proposed by Szidat (1950) from Tierra del Fuego province (~ 54° S), Argentina. This digenean uses the Patagonian blennie as definitive host, and the intertidal bivalve Lasaea adansoni (Gmelin) (Lasaeidae) as both first and second intermediate hosts (metacercariae encyst inside sporocysts), being the first record of this clam as intermediate host of trematode parasites. The cercaria may, in addition to encysting in the sporocyst, emerge and presumably infect other intermediate hosts. This is the second report of a monorchiid species with metacercariae encysting inside the sporocyst. Adults were found parasitizing the fish stomach, pyloric caeca and intestine with a prevalence of 100%; sporocysts with cercariae and\/or metacercariae were found parasitizing the gonad of the bivalve with a prevalence of 2.78%. The cercariae possess a well-developed tail and eye-spots are absent. The ITS1 sequence from the adult digeneans found in the Patagonian blennie, identified as P. maclovini, was found to be identical to the ITS1 sequences obtained both from sporocysts containing cercariae and encysted metacercariae found in L. adansoni.","palabras_clave":"LIFE CYCLE|MONORCHIIDAE|POSTMONORCHEIDES MACLOVINI|LASAEA ADANSONI|ELEGINOPS MACLOVINUS|PATAGONIAN COAST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3684,"idioma_id":4,"titulo":"Purification of two bacteriocins produced by Enterococcus faecalis DBFIQ E24 strain isolated from raw bovine milk","resumen":"Enterococcus faecalis DBFIQ E24 was formerly characterised as a producer of antibacterial and antifungal substances. This strain is vancomycin sensitive, nonhaemolytic and gelatinase negative. SDS-PAGE revealed the presence of an active band located between 1060 and 3500&nbsp;Da. After the application of a three-step purification procedure, two antimicrobial peptides were isolated. One of them is mostly active against Bacillus cereus with a molecular mass of 1364&nbsp;Da, and the other one with a molecular mass of 1686&nbsp;Da is mainly active against Escherichia coli. These results confirm the technological potential of E.&nbsp;faecalis DBFIQ E24 strain as a GRAS food biopreservative.","palabras_clave":"ENTEROCOCCUS FAECALIS STRAIN |ENTEROCIN|PURIFICATION|FOOD BIOPRESERVATIVE|WIDE INHIBITORY SPECTRA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3685,"idioma_id":4,"titulo":"Screening and characterization of potential probiotic and  starter bacteria for plant fermentations","resumen":"Probiotics are mostly consumed as fermented or fortified food products in Europe. There are twoimportant factors in the selection of probiotic candidates regarding the potential health benefit; theirviability and number when consumed, and their survival and persistence in the gastrointestinal tracts.This study focusses on the selection of potential probiotics to be used as starter culture in plant-basedfermented foods. Lactic acid bacteria isolated from quinoa and amaranth were tested in vitro for theirsensitivity to antibiotics, tolerance to gastrointestinal stress factors and adhesion to gut epithelial cells.Only five strains had suitable antibiotic profile to be used as probiotics and all of them were tolerant tolysozyme, bile salts, and had similar adhesion capacity. Lactobacillus plantarum Q823, administered asstarter culture in a fermented quinoa drink, was selected for the human in vivo tests, because of its bestin vitro tolerance to low pH. This strain was able to survive and persist at detectable levels (5e7Log10 CFU\/g feces) in the gastrointestinal tracts for at least seven days after the end of administration.Thus, L. plantarum Q823 has been identified as a suitable starter and a potential probiotic in fermentedquinoa-based products.","palabras_clave":"PROBIOTIC BACTERIA|CARACTERIZATION|COLONIZATION|FERMENTED PLANT MATERIAL|QUINOA AND AMARANTH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3686,"idioma_id":4,"titulo":"Virulence characterization and identification of resistant maize lines to Puccinia sorghi Schwein present in the Argentine Corn Belt region","resumen":"Puccinia sorghi&nbsp;Schwein., the causal agent of maize common rust, is an endemic disease in the Argentine Corn Belt region. Virulence surveys of the pathogen population within the region have not been performed; thus, the understanding of the pathogen population is low and it is difficult to deploy resistance genes that could be effective at controlling the disease. In total, 58 single-uredinial isolates derived from infected maize leaves collected in different locations throughout the Argentine Corn Belt region during 2010 to 2012 were tested on a set of 25 maize lines carrying different&nbsp;Rp&nbsp;genes. Maize lines&nbsp;Rp3-A&nbsp;and PIO19802 showed the lowest virulence frequencies (3.4 and 1.7%, respectively) for all tested isolates. Moreover, the combination in a single genotype of the resistance genes carried by lines&nbsp;Rp3-A&nbsp;and PIO19802 or either of these lines combined with the resistance genes from PIO12345 would confer resistance to all isolates tested. Virulent isolates on maize lines&nbsp;Rp-G,&nbsp;Rp1-K, and&nbsp;Rp-GI&nbsp;were most frequent in 2012. Twenty-four virulence phenotypes were identified, with phenotypes TCCG (17.2%), TTBB (15.5%), and TCFG (10.3%) being the most common throughout the region. Adult plant resistance associated with hypersensitive response was identified at vegetative stage 6 in maize lines PIO68752, PIO28427, and PIO36420.","palabras_clave":"MAIZE|RESISTANCE GENE|VIRULENCE|PUCCINA SORGHI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3687,"idioma_id":4,"titulo":"How the Cation 1-butyl-3-methylimidazolium Impacts on the Interaction between the Entrapped Water and the Reverse Micelles Interface Created with an Ionic Liquid-like Surfactant","resumen":"The behavior of the interfacial water entrapped in reverse micelles (RMs) formed by the ionic liquid-like surfactant 1-butyl-3-methylimidazolium 1,4-bis-2-ethylhexylsulfosuccinate (bmim-AOT) dissolved in benzene (or chlorobenzene) was investigated using noninvasive techniques such as dynamic light scattering (DLS), static light scattering (SLS), FT-IR and 1H NMR. The DLS and SLS results reveal the formation of discrete spherical and non-interacting water droplets stabilized by the bmim-AOT surfactant. Moreover, since the droplet size increases as the W0 (W0 = [water]\/[surfactant]) value increases, water interacts with the RM interface. From FT-IR and 1H NMR data, a weaker water-surfactant interaction in bmim-AOT RMs in comparison with the RMs created by sodium 1,4-bis-2-ethylhexylsulfosuccinate (Na-AOT) is detected. Consequently, there are less water molecules interacting with the interface in bmim-AOT RMs, and their hydrogen bond network is not completely disrupted as they are in Na-AOT RMs. The results show how the nature of the new cation impacts the interaction between the entrapped water and the RM interface, modifying the interfacial water structure in comparison with the results known for Na-AOT.","palabras_clave":"IONIC LIQUID|REVERSE MICELLES|FTIR|NMR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3688,"idioma_id":4,"titulo":"ABA and GA3 increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves, enhancement of phloem area and expression of sugar transporters","resumen":"Grape quality for winemaking depends on sugar accumulation and   metabolism in berries. Abscisic acid (ABA) and gibberellins (GAs) have   been reported to control sugar allocation in economically important   crops, although the mechanisms involved are still unknown. The present   study tested if ABA and gibberellin A3 (GA3)   enhance carbon allocation in fruits of grapevines by modifying phloem   loading, phloem area and expression of sugar transporters in leaves and   berries. Pot-grown Vitis vinifera cv. Malbec plants were sprayed with ABA and GA3   solutions. The amount of soluble sugars in leaves and berries related   to photosynthesis were examined at three points of berry growth:   pre-veraison, full veraison and post-veraison. Starch levels and amylase   activity in leaves, gene expression of sugar transporters in leaves and   berries and phloem anatomy were examined at full veraison. Accumulation   of glucose and fructose in berries was hastened in ABA-treated plants   at the stage of full veraison, which was correlated with enhancement of Vitis vinifera HEXOSE TRANSPORTER 2 (VvHT2) and Vitis vinifera HEXOSE TRANSPORTER 6 (VvHT6) gene expression, increases of phloem area and sucrose content in leaves. On the other hand, GA3   increased the quantity of photoassimilates delivered to the stem thus   increasing xylem growth. In conclusion, stimulation of sugar transport   by ABA and GA3 to berries and stems, respectively, was due to   build-up of non-structural carbohydrates in leaves, modifications in   phloem tissue and modulation in gene expression of sugar transporters.","palabras_clave":"Grapevine|ABA|Sugar metabolism"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3689,"idioma_id":4,"titulo":"Comparative behavior of protein or polysaccharide stabilized emulsion under in vitro gastrointestinal conditions","resumen":"In the present work, an in vitro model digestion was used to compare the behavior of emulsions stabilizedby proteins or polysaccharide upon digestion and to analyze its relationship with the kinetics andextent of lipid digestion. Oil\/water emulsions were prepared using different emulsifiers (b-lactoglobulin,soy protein isolate and hydroxypropylmethylcellulose (HPMC)). The emulsion digestion was carried outin two continuous stages at 37 C: 1) under simulated gastric conditions (1 h) using pepsin and phosphatidylcholine (simulated gastric fluid: pH 2.5, NaCl, NaH2PO4, KCl and CaCl2) and 2) under simulatedintestinal conditions (1 h) with bile salts, pancreatic lipase, trypsin and chymotrypsin (simulated intestinal fluid: pH 7.0, K2HPO4, NaCl and CaCl2). The changes in the particle size distributions, theinterfacial area and their microstructures were analyzed as a function of the digestion time. The free fatty acid release during the simulated intestinal stage was also determined and an empirical model was fitted to estimate different kinetic parameters. Irrespective of the composition\/structure of emulsions, the initial surface area was found to determine the initial rate of lipolysis. Soy protein was the protein that forms the most resistant emulsion to digestion, showing a degree of free fatty acid release similar to HPMC, which is a non digestible emulsifier. The results are discussed on the basis of the role of bile salts and its effect on oil\/water interfaces.","palabras_clave":"EMULSION|GASTROINTESTINAL DIGESTION|PROTEIN|POLYSACCHARIDE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3690,"idioma_id":4,"titulo":"Aqueous two-phase systems: A simple methodology to obtain mixtures enriched in main toxins of Bothrops alternatus venom","resumen":"Phospholipase A2 (PLA2) and protease (P) are enzymes responsible of myotoxic, edematogenic and hemostasis disorder effects observed in the envenomation byBothrops alternatus pitviper. Their partitioning coefficient (Kp) in different polyethyleneglycol\/potassium phosphate aqueous two-phase systems (ATPSs) was determined in order to both achieve a better understanding of the partitioning mechanism and define optimal conditions for toxin isolation. Polyethyleneglycols (PEGs) of molecular weights 1000; 3350; 6000 and 8000; different temperatures (5, 20 and 37 °C) and phase volume ratios of 0.5; 1 and 2 were assayed. PLA2 partitioned preferentially to the top phase while P mainly distributed to the bottom phase. Either entropically- or enthalpically-driven mechanisms were involved in each case (PLA2 and P). The aqueous two-phase system formed by PEG of MW 3350 (12.20% wt\/wt) and KPi pH 7.0 (11.82% wt\/wt) with a volume ratio of one and a load of 1.25 mg of venom\/g of system showed to be the most efficient to recover both enzymes. It allowed obtaining the 72% of PLA2 in the top phase with a purification factor of 2 and the 82% of P at the bottom phase simultaneously. A further adsorption batch step with DEAE-cellulose was used to remove satisfactorily the PEG from the top phase and recover the active PLA2. The proposed methodology is simple, inexpensive, and only requires professionals trained in handling basic laboratory equipment. It could be easily adoptable by developing countries in which the snakebite accidents cause considerable morbidity and mortality.","palabras_clave":"Aqueous two-phase systems|Snake venom|Phospholipase A2|Protease"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3691,"idioma_id":4,"titulo":"Anemia and Hyperplastic Gastritis in a Giant  Anteater ( Myrmecophaga tridactyla ) due to  Physaloptera magnipapilla Parasitism","resumen":"A free living adult female of Myrmecophaga tridactyla was captured in an area of deforestation in Salta Province of northwest Argentina, for subsequent relocation in Iberá Park. Three days after entering the quarantine died with clinical signs of anorexia and ascites.The clinical pathology analysis showed severe hypochromic microcytic anemia, hypoproteinemia and increase of CPK, AST and LDH. In the necropsy was found ascites, hydrothorax, pulmonary congestion and severe catarrhal hyperplasic gastritis. The gastric mucosa was diffusely hyperemic with two clusters of numerous Physaloptera magnipapilla attached in the fundic region. Fundic mucosa was irregular with convoluted surface and covered with abundant brown-yellow catarrhal exudates. The microscopic lesions in gastric mucosa was micro-hole at points of attachment of P. magnipapilla with complete loss of the mucosa until muscularis mucosae, mucous hyperplasia of gastric glands surroundings to P. magnipapilla and focal fibrosis with scarce infiltration of plasma cells, lymphocytes, multinucleated giant cells and eosinophils in the lamina propria underlying. Physaloptera magnipapilla can cause anemia and chronic hyperplasic catarrhal gastritis in free living Myrmecophaga tridactyla.","palabras_clave":"Physalopteriasis|Giant anteater|Gastritis|Hyperplastic catarrhal gastritis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3692,"idioma_id":4,"titulo":"Matrix metalloproteinases and psychosocial factors in acute coronary syndrome patients","resumen":"tPsychosocial factors have been linked to cardiovascular diseases independently of traditional risk factors.The impact of psychosocial factors on plaque destabilizing factors, such as matrix metalloproteinases(MMPs) has been proposed although scarcely studied.Objective: To evaluate the relationships between hostility, perceived stress and social support with MMPsactivity in patients after an Acute Myocardial Infarction (AMI).Methods: Blood samples were obtained from 76 patients on admission, post-angioplasty, 24 h, 7 daysand 3 months after AMI. Hostility, perceived stress and social support were evaluated by validatedquestionnaires.Results: Social support was positively correlated with patients´ý ejection fraction (r = 0.453, p = 0.009).Patients with higher infarct size presented increased MMP-2 activity at admission (p = 0.04). Patientswith one diseased vessel had more social support than those with three diseased vessels (p = 0.05). Thehighest values of MMP-2 and MMP-9 activity were observed at the acute event, decreasing, with thelowest activity at 3 months post-AMI (p &lt; 0.001). Only in patients with low social support, hostility corre-lated with MMP-2 activity, from AMI onset (r = 0.645, p = 0.013), to 7 days post AMI (r = 0.557, p = 0.038).Hostility explained up to 28% of the variance in MMP-2 activity (R2= 0.28, p = 0.005). Finally, in patientswith high hostility, MMP-9 was positively correlated with IL-1  (r = 0.468, p = 0.02).Conclusions: This study adds weight to the idea that two psychosocial factors, namely hostility and socialsupport, acting jointly, may affect MMP-2 activity. Moreover, in hostile patients, there is a link betweenIL-1  and MMP-9. These findings support the role of psychosocial factors in plaque destabilization andin the inflammatory process in AMI.","palabras_clave":"METALLOPROTEINASE|ACUTE CORONARY SYNDROME|SOCIAL SUPPORT|HOSTILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3693,"idioma_id":4,"titulo":"Characterization of sintered mixed oxides by Photothermal Microscopy","resumen":"A new photothermal microscopy device is presented and used to map the thermal diffusivity of mixed oxides. The particular cases of UO2+10%Gd2O3 and UO2+7.5%Dy2O3 are discussed, showing how to determine the homogeneity of the prepared samples, distinguish interdiffused oxides from mixed oxides, recognize and characterize clusters and determine the relative abundance of different species and its spatial distribution. Histograms can be generated that provide a quantitative determination of the homogeneity. Adding an independent determination of the thermal diffusivity as a function of the composition made in bulk material allows the conversion of the diffusivity map into a concentration map, providing direct quantitative determination of the homogeneity and spatial distribution in concentration units.  The new device can be added as an accessory to an optical microscope entering through a standard C mount camera port. Two semiconductor lasers within the viewing spectroscopic window of any microscope (650nm wavelength for the pump and 785nm for the probe) that were coupled to a single mode fiber were used, and the reflected signal was collected using the same fiber. The system resulted rugged and robust.","palabras_clave":"photothermal images|diffusivity map|concentration map|interdiffused oxides|urania|dysprosia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3694,"idioma_id":4,"titulo":"Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM\/CD166) promotes its surface segregation on breast cancer cells","resumen":"Background: We previously demonstrated that the activated leukocyte cell adhesion molecule (ALCAM\/CD166) can interactwith galectin-8 (Gal-8) in endothelial cells. ALCAM is a member of the immunoglobulin superfamily that promotes homophilic and heterophilic cell-cell interactions. Gal-8 is a 'tandem-repeat'-type galectin, known as a matricellular protein involved in cell adhesion. Here, we analyzed the physical interaction between both molecules in breast cancer cells and the functional relevance of this phenomenon.Methods: We performed binding assays by surface plasmon resonance to study the interaction between Gal-8 and the recombinant glycosylated ALCAM ectodomain or endogenous ALCAM from MDA-MB-231 breast cancer cells. We also analyzed the binding of ALCAM-silenced or control breast cancer cells to immobilized Gal-8 by SPR. In internalization assays, we evaluated the influence of Gal-8 on ALCAM surface localization.Results: We showed that recombinant glycosylated ALCAM and endogenous ALCAM from breast carcinoma cells physically interacted with Gal-8 in a glycosylation-dependent fashion displaying a differential behavior compared to non-glycosylated ALCAM. Moreover, ALCAM-silenced breast cancer cells exhibited reduced binding to Gal-8 relative to control cells. Importantly, exogenously added Gal-8 provoked ALCAM segregation, probably trapping this adhesion molecule at the surface of breast cancer cells.Conclusions: Our data indicate that Gal-8 interacts with ALCAM at the surface of breast cancer cells through glycosylation-dependent mechanisms.General significance: A novel heterophilic interaction between ALCAM and Gal-8 is demonstrated here, suggesting its physiologic relevance in the biology of breast cancer cells.","palabras_clave":"GALECTIN-8|ALCAM|BREAST CARCINOMA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3695,"idioma_id":4,"titulo":"A contourite depositional system along the Uruguayan continental margin: sedimentary, oceanographic and paleoceanographic implications","resumen":"Procesos sedimentarios contorniticos y turbiditicos en margen continental de  Uruguay","palabras_clave":"contourites|uruguay"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3696,"idioma_id":5,"titulo":"MONOAMBIENTES DEL PASADO. ESTRATEGIAS DE REPRODUCCIÓN SOCIAL EN UNIDADES DOMÉSTICAS DE PUEBLO VIEJO DE TUCUTE","resumen":"Ubicado en la Puna de Jujuy, Pueblo Viejo de Tucute presenta fechados que lo ubicanen el Periodo de Desarrollos Regionales y un patrón arquitectónico distintivo parala región, al poseer cientos de recintos habitacionales circulares construidos sobreterrazas niveladas con muros de contención. Hasta el momento se han sondeado 9 deestos recintos, dos de ellos excavados de manera total. La comparación de éstos, tantodesde su emplazamiento en el sitio como la materialidad asociada, permite plantearsimilitudes y diferencias en cuanto al uso y organización de los espacios domésticos ytambién indagar sobre el status social de los habitantes de ambas unidades. Medianteel estudio de los componentes arquitectónicos fijos y semifijos, sumado al análisisartefactual, se ha logrado reconocer diferentes actividades vinculadas con estrategiasde reproducción económica y social, tanto aspectos biológicos y materiales básicos,actividades productivas y otras relacionadas con prácticas simbólicas asociadas alsostenimiento de relaciones a nivel comunitario. A estos datos se agregan aportesde cronistas coloniales y registros etnográficos del altiplano boliviano, de la primeramitad del siglo XX, que describieron aspectos de la forma de vida y el uso de recintoscon características similares.","palabras_clave":"ESPACIOS DOMÉSTICOS|ARQUITECTURA ARQUEOLOGICA|REPRODUCCIÓN SOCIAL|PUNA DE JUJUY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3697,"idioma_id":4,"titulo":"Use of citrus flavanones to prevent aflatoxin contamination using response surface methodology","resumen":"The aim of this study was to analyze the possible utilization of flavanones obtained as by-products of the citrus industry, naringin (NAR), hesperidin (HES) and neohesperidin (NEO), to inhibit the production of aflatoxins (AFs) from Aspergillus flavus. Response Surface Methodology (RSM) was applied to optimizeexperimental conditions in terms of the different flavanones concentrations used. Through this methodology these optimal combinations were calculated: HES-NAR: 0.206e0.037 mM, HES-NEO: 0.156 e0.283 mM and NAR-NEO: 0.035e0.195 mM. The theoretical concentrations obtained by RSM were assayed, achieving total inhibition of AFB1 and AFB2 production. Moreover, the use of these flavanones, obtained at low cost from the residues of citric industry, presents an interesting option for improving the profitability of these industries.","palabras_clave":"FLAVONONES|ASPERGILLUS FLAVUS|AFLATOXINS|RESPONSE SURFACE METHODOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3698,"idioma_id":4,"titulo":"Surface ages of mid-size saturnian satellites","resumen":"The observations of the surfaces of the mid-sized saturnian satellites made by Cassini-Huygens mission have shown a variety of features that allows study of the processes that took place and are taking place on those worlds. Research of the saturnian satellite surfaces has clear implications not only for Saturn´s history and Saturn´s surroundings, but also for the Solar System. Crater counting from high definition images is very important and could serve for the determination of the age of the surfaces. In a recent paper, we have calculated the production of craters on the mid-sized saturnian satellites by Centaur objects considering the current configuration of the Solar System. Also, we have compared our results with crater counts from Cassini images by other authors and we have noted that the number of observed small craters is less than our calculated theoretical number. In this paper we estimate the age of the surface for each observed terrain on each mid-sized satellite of Saturn. All the surfaces analyzed appear to be old with the exception of Enceladus. However, we have noticed that since there are less observed small craters than calculated (except on Iapetus), this results in younger ages than expected. This could be the result of efficient endogenous or exogenous process(es) for erasing small craters and\/or crater saturation at those sizes. The size limit from which the observed number of smaller craters is less than the calculated is different for each satellite, possibly indicating processes that are unique to each, but other potential common explanations for this paucity of small craters would be crater saturation and\/or deposition of E-ring particles. These processes are also suggested by the findings that the smaller craters are being preferentially removed, and the erasure process is gradual.On Enceladus, only mid and high latitude plains have remnants of old terrains; the other regions could be young. In particular, the regions near the South Polar Terrain could be as young as 50&nbsp;Myr old. On the contrary for Iapetus, all the surface is old and it notably registers a primordial source of craters. As the crater size is decreased, it would be perceived to approach saturation until D&#8818;2 km-craters, where saturation is complete.","palabras_clave":"SATURN, SATELLITES|CRATERING|CENTAURS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3699,"idioma_id":4,"titulo":"Robust Control of Wiener Systems: Application to a Ph Neutralization Process","resumen":"In this paper, the robustness of a typical control scheme for Wiener systems is studied. These systems consist of the cascade connection of a linear time invariant system and a static nonlinearity. To control this kind of systems, several approaches were discussed in the literature. Most of these control schemes involve transformation of the measured variable as well as the setpoint, by the inverse of the nonlinear gain.The approach followed in this work uses the inverse model of the static nonlinear gain, while the uncertainty in the Wiener model is treated as a partitioned problem. The linear block is considered as a parameter-affine-dependent model and, on the other hand, the nonlinear block uncertainty is analyzed as a conic-sector. The robustness analysis is performed using &#956;-theory.The results are evaluated on the basis of a simulation of a pH neutralization process.","palabras_clave":"Wiener Models|Process control|Uncertainty|Robustness"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3700,"idioma_id":5,"titulo":"Compartimentalización de la biomasa aérea de Grevillea robusta A. en función a la densidad inicial de plantación","resumen":"A densidade de plantação a uma determinada idade é um indicador confiável da ocupação arbórea de um local, representando assim a estrutura das florestas. O objetivo deste estudo foi avaliar o efeito da densidade de plantio inicial no acúmulo da biomassa de plantações de Grevillea robusta a os 18 anos de idade. O ensaio foi conduzido em Posadas Misiones, Argentina, com 4 densidades de plantio iniciais (162, 375, 750 e 1500 plantas \/ ha). O acúmulo da biomassa foi analisado nos estratos arbustivo, herbáceo, piso florestal e arbóreo (folhas, galhos, tronco, casca). Nas árvores é considerado tanto o efeito sobre os indivíduos e sobre o sítio. A biomassa total do sub-bosque apresentou diferenças significativas entre os tratamentos. A distribuição da biomassa variou resultando em aumento da presença de galhos mortos e biomassa do estrato arbustivo em densidades de plantio superiores. A acumulação da biomassa herbácea foi maior no tratamento da plantação da menor densidade. O maior espaçamento resultou num maior diâmetro dos indivíduos, mas a maior acumulação da biomassa do fuste por hectare foi em espaçamentos menores.","palabras_clave":"Distribuição da biomassa|sub-bosque|estrato arbóreo|espaçamento|manejo florestal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3701,"idioma_id":4,"titulo":"Photophysics of an octasubstituted zinc(II) phthalocyanine incorporated into solid polymeric magnetic and non-magnetic PLGA-PVA nanoparticles","resumen":"2,3,9,10,16,17,23,24-octakis[(N,N-dimethylaminoethylsulfanyl)]phthalocyaninatozinc (II) (S1) was usedas a lipophilic photosensitizer to be encapsulated into biodegradable poly(D,L lactide-co-glycolide)-polyvinyl alcohol nanoparticles. The synthesis, size, and spectroscopic, photophysical, and magneticproperties of nanoparticles, were analyzed. A greater number of sonication cycles afforded smallernanoparticles. The stabilization by means of polyvinyl alcohol of all the nanoparticles synthesizedprovided a negative charge to the systems in a wide pH range. The thermograms obtained by differentialscanning calorimetry showed that the nanoparticles were stabilized by polyvinyl alcohol. Theincorporation of magnetite nanoparticles was verified by FT-IR, magnetization measurements by meansof hysteresis curves and potential Z. S1 retained its efficiency in generating singlet oxygen. Theincorporation of magnetite into the nanoparticles showed an increase in singlet oxygen quantum yieldsdue to the heavy atom effect.","palabras_clave":"PHTHALOCYANINIES|PLGA- PVA NANOPARTICLES|MAGNETITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3702,"idioma_id":4,"titulo":"Bi-Magnetic Iron (III) Oxide Nanocrystals Embedded in MCM-41 Mesoporous Silica","resumen":"ABSTRACT: This is a study on the magnetism of a Fe2O3@MCM-41composite made up of iron(III) oxide nanocrystals of average length sizeof 4 nm embedded in the channels of a MCM-41 mesoporous matrix.Through Mo&#776;ssbauer spectroscopy, X-ray absorption experiments andstatic and dynamic magnetic measurements, we identify the guest ironoxide entities as composed by exchange-coupled hematite (weakferromagnetic WF) and maghemite (ferrimagnetic FiM) arranged in astring of beads configuration. The WF\/FiM nanocrystals behave asindependent particle moments that block as a whole below TB = 17 K.The log-normal energy barrier distribution function determined fromrelaxation measurements shows a maximum at an energy E = 200 K. Weexplore to what extent the contact between domains of the twocoexisting oxides controls the magnetism of this biphasic nanosystem","palabras_clave":"NANOPARTICLES|MAGNETISM|OXIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3703,"idioma_id":4,"titulo":". A new method for simultaneous detection and discrimination of Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) using real time PCR with high resolution melting (HRM) analysis.","resumen":"Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are antigenically and genetically similar. The aim of this study was to develop a simple and reliable one-step real time PCR assay with high resolution melting (HRM) analysis for the simultaneous detection and differentiation of BoHV-1 and BoHV-5. Optimization of assay conditions was performed with DNA from reference strains. Then, DNA from field isolates, clinical samples and tissue samples of experimentally infected animals were studied by real time PCR-HRM. An efficient amplification of real time PCR products was obtained, and a clear melting curve and appropriatemelting peaks for both viruses were achieved in the HRM curve analysis for BoHV type identification. BoHV was identified in all of the isolates and clinical samples, and BoHV types were properly differentiated. Furthermore, viral DNA was detected in 12\/18 and 7\/18 samples from BoHV-1- and BoHV-5-infectedcalves, respectively. Real time PCR-HRM achieved a higher sensitivity compared with virus isolation or conventional PCR. In this study, HRM was used as a novel procedure. This method provides rapid, sensitive, specific and simultaneous detection of bovine alpha-herpesviruses DNA. Thus, this technique is anexcellent tool for diagnosis, research and epidemiological studies of these viruses in cattle.","palabras_clave":"Bovine herpesvirus|High resolution melting|Molecular detection|Real Time PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3704,"idioma_id":4,"titulo":"Contribution of Reserves to Kernel Weight and Grain Yield Determination in Maize: Phenotypic and Genotypic Variation","resumen":"Maize (Zea mays L.) kernel weight (KW) and grain yield depend on plant growth during active grain filling and reserves use. The objective of our study was to analyze the phenotypic and genotypic variation in these traits in a family of recombinant inbred lines (RIL). In two field experiments we measured plant grain yield (PGY) and its components (KW and kernel number per plant, KNP), biomass production per plant and per kernel during active grain filling, and apparent reserves use (ARU) per plant (ARUP: difference between PGY and plant biomass production during active grain filling) and per kernel (ARUK: difference between KW and plant biomass production per kernel during active grain filling). Heritability (h 2) and phenotypic plasticity were computed for all traits. Large differences were always evident among genotypes, but phenotypic plasticity was (i) low for KW, and plant biomass at R2 and physiological maturity, (ii) intermediate for KNP and PGY, and (iii) high for plant growth, plant growth per kernel after R2, and ARUs. Traits with highest h 2 were KW (0.70), KNP (0.61) and ARUP (0.59). Final KW was related to plant growth per kernel (r 2 = 0.64; P  popcorn). In summary, current knowledge on ARUP and ARUK during maize grain filling is not conclusive about the relevance of these traits for KW and yield determination and on Crop Sci. available genotypic variation. The objective of our study was to quantify and analyze ARUs. For this purpose we used a family of recombinant inbred lines (RIL) derived from the cross of two inbreds with contrasting genetic background.","palabras_clave":"kernel weight |maize|recombinant inbred lines"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3705,"idioma_id":4,"titulo":"Percepciones y expectativas sobre el contexto de aprendizaje. Un estudio con alumnos de Educación Superior.","resumen":"Este artículo expone las principales caracteríticas y resultados de un estudio orientado a conocer las percepciones y expectativas sobre los contextos de aprendizaje de tres grupos de estudiantes que participaron de procesos de aprendizaje en educación superior -terciario no universitario, universitario de grado y universitario de posgrado-. Se trabajó con 303 estudiantes que cursaron estudios superiores y los datos fueron recabados por medio del Inventario de Percepción sobre Contextos de Aprendizaje (IPSE, Inventory of Perceived Study Environment) de Wierstra, Kanselaar, van der Linden & Lodeewijks (1999). Los resultados muestran diferencias significativas entre las percepciones y las expectativas de los grupos de estudiantes sobre los contextos de aprendizaje. Los hallazgos de este trabajo son interesantes para pensar la configuración de nuevos diseños educativos. SCOPUS, SCIELO, SJR- SCIMAGO , Redalyc, PsyInfo, Google Scholar,DOAJ,Pepsic","palabras_clave":"PERCEPCIONES|EXPECTATIVAS|EDUCACIÓN SUPERIOR|CONTEXTOS DE APRENDIZAJE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3706,"idioma_id":4,"titulo":"Joint interpretation of the hydrochemistry of two neighbouring basins by N-way multivariate methods","resumen":"The objective of this work is to compare the chemical composition and the spatial and temporal variability of groundwater in two basins, the Langueyú and Del Azul creeks basins, located in the Pampean plain, Buenos Aires province (Argentina). The Pampean plain is the most productive region in Argentina, agriculture and livestock being the main economic activities. Groundwater is the principal water resource in the region, with a strong and growing demand for human supply and for agriculture and industrial activities. Several sampling campaigns were carried out on shallow wells of the two studied basins along a period of three years (2010-2013) in order to identify seasonal variations. Electrical conductivity, pH, bicarbonate, chloride, sulphate, nitrate, calcium, magnesium, sodium and potassium were determined following standard methods. For hydrochemical interpretation, descriptive statistical analyses, matrixaugmentation principal component analysis, MA-PCA, and multidimensional Principal component analysis, N-PCA (Parafac and Tucker3 models), were applied to the hydrochemical datasets from both basins. Three main hydrochemical processes have been identified in both basins: saline enrichment in the groundwater flow direction caused by dissolution of carbonates; exchange of calcium and magnesium by sodium in the same direction, and located areas of nitrate pollution. The paper shows that NPCA is a good tool to deepen in the understanding of the hydrochemical features of groundwater from two neighbour basins, simplifying the analysis of large amounts of data, as well as establishing relations between the compared basins. Therefore thework is considered an interesting contribution to the study of groundwater resources with a regional scope. This knowledge is essential in basins with high socio-economic interests it causes a direct impact on resources management.","palabras_clave":"HYDROCHEMISTRY|GROUNDWATER|MULTIVARIATE METHODS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3707,"idioma_id":5,"titulo":"Magnitud y variación de la mastitis clínica y sus costos asociados en rodeos lecheros de Argentina","resumen":"Se llevó a cabo un estudio para evaluar la magnitud y variación de la mastitis clínica en predios lecheros. Se estimaron la prevalencia, incidencia acumulada y tasa de incidencia, explorando factores de riesgo a nivel de vaca y las consecuencias económicas de los casos clínicos. Una muestra de conveniencia de 2.535 vacas Holstein-Friesian en lactación de 19 rodeos lecheros constituyó la población en estudio. En cada predio, la prevalencia fue estimada el día de la visita. Las vacas sin mastitis fueron monitoreadas 15 días para estimar la incidencia. Se aplicaron modelos logísticos y de supervivencia mixtos. Los casos prevalentes fueron valuados considerando la leche descartada, tratamiento y disminución de la producción en el resto de la lactancia. La prevalencia cruda fue de 2,5 casos\/100 vacas (IC 95% = 1,9-3,1), la incidencia acumulada de 3,9 casos nuevos\/100 vacas (IC 95% = 3,1-4,7) y la tasa de incidencia de 2,7 casos nuevos\/1.000 vacas-días a riesgo (IC 95% = 2,2-3,3). La mediana de rodeo fue de 1,69 (q1 = 0; q3 = 4,2), 3,6 (q1 = 2,0; q3 = 4,6) y 0,25 (q1 = 0,14; q3 = 0,42), respectivamente. Los casos fueron más frecuentes en lactancia temprana (OR = 1,69; IC 95% = 1,01-2,85). En vacas, tanto la incidencia acumulada (OR = 3,36; IC 95% = 1,84-6,11) como la tasa de incidencia (HR = 3,83; IC 95% = 2,05-7,15) fueron tres veces mayores. El costo de un caso fue de US$ 185,01 y US$ 143,03 para vacas y vaquillonas, respectivamente. La pérdida promedio fue de 4,50 US$\/vaca en ordeña (IC 95% = 3,38-5,61). Este es el primer estudio que aborda la distribución e impacto de la mastitis clínica en rodeos lecheros en Argentina.","palabras_clave":"clinical mastitis,|prevalence|incidence|economic losses"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3708,"idioma_id":4,"titulo":"Supernumerary (B) chromosomes in direct-developing frogs of the genus Oreobates (Brachycephaloidea: Craugastoridae)","resumen":"Oreobates Jiménez de la Espada, 1872 is a South American frog genus within the superfamily Brachycephaloidea, a large group of frogs with terrestrial reproduction and direct development. About 260 brachycephaloidean species have been cytogenetically studied so far, at least with standard techniques. However, this information represents fewer than 17% species of the family Craugastoridae Hedges, Duellman and Heinicke, 2008, where the genus Oreobates is included. In the present work, using a diversity of standard and molecular techniques, we describe the karyotype of O. barituensis Vaira and Ferrari, 2008, O. berdemenos Pereyra, Cardozo, Baldo, and Baldo, 2014 and O. discoidalis (Peracca, 1895), from northwestern Argentina. The three species analyzed showed a diploid karyotype with 2n = 22 biarmed chromosomes, fundamental number (FN) = 44, nucleolus organizer regions (NORs) located pericentromerically on pair 7, and a centromeric and perocentromeric C-banding pattern. We observed variations in the chromosome number in O. barituensis due the presence of two morphs of B chromosomes, one medium-sized telocentric (BT) and another subtelocentric and smaller (Bst). Both B chromosomes are mitotically stable and were recorded in all somatic and germinal cells analyzed. The BT chromosome occurred at a maximum of one per individual (2n = 22+BT), and the other one was observed single (2n = 22 + Bst) or as a pair in two doses (2n = 22 + 2BT). We additionally observed other supernumerary chromosomes in the three species analyzed, all of them euchromatic, small, dot-shaped and with instability during mitoses, showing a frequency of occurrence below 50% in studied specimens. The occurrence of polymorphic and spontaneous chromosomal rearrangements and supernumerary chromosomes is a recurrent feature reported in frogs with terrestrial habits (Brachycephaloidea and Hemiphractidae Peters, 1862), which suggests that Brachycephaloidea may be a promising group for studying the origin and maintenance of B chromosomes in anurans.","palabras_clave":"Cytogenetics|accessory elements|ribosomal DNA|Anura"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3709,"idioma_id":4,"titulo":"GENOMIC DIVERSITY OF PHAGES INFECTING PROBIOTIC STRAINS OF Lactobacillus paracasei","resumen":"Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: CL1, CL2, iLp84, iLp1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (CL1). Phages iA2 and iLp1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages CL1, CL2, and iLp1308 are highly related to each other. Phage iLp84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages CL1, CL2, iLp1308, and iLp84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.","palabras_clave":"BACTERIOPHAGES|LACTOBACILLUS PARACSEI|PROBIOTIC STRAINS|GENOMIC DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3710,"idioma_id":4,"titulo":"Natural origin arsenic in aquatic organisms from a deep oligotrophic lake under the influence of volcanic eruptions","resumen":"Volcanic eruptions are recognized sourcesof toxic elements to freshwater, including arsenic (As). In order to study theshort term changes in the bioaccumulation of naturally occurring As by aquaticorganisms in Lake Nahuel Huapi (Argentina), located close to the Puyehue-CordónCaulle volcanic complex (PCCVC), we described As concentrations at differenttrophic levels and food web transfer patterns in three sites of the lake priorto the last PCCVC eruption (June 2011), and compared As concentrations in biotabefore and after the eruption. The highest As concentrations and greatervariations both between sites and position in the water column, were observedin phytoplankton (3.9-64.8 µg g-1 dry weight, DW) and small zooplankton (4.3-22.3 µg g-1DW). The pattern of As accumulation in aquatic organisms (whole body or muscle)was: primary producers (phytoplankton) &gt; scrapper mollusks (9.3-15.3 µg g-1 DW)&gt; filter feeding mollusks (5.4-15.6 µg g-1 DW) &gt; omnivorousinvertebrates (0.4-9.2 µg g-1 DW) &gt; zooplankton (1.2-3.5 µg g-1 DW) &gt; fish (0.2-1.9 µg g-1 DW). We observed Asbiodilution in the whole food web, and in salmonids food chains, feeding on fishprey; but biomagnification in the food chain of creole perch, feeding onbenthic crayfish. The impact of the 2011 PCCVC eruption on the As levels ofbiota was more evident in pelagic-associated organisms (zooplankton andplanktivorous fish), but only in the short term, suggesting a brief highbioavailability of As in water after ash deposition. In benthic organisms Asvariations likely responded to shift in diet due to coverage of the littoralzone with ashes.","palabras_clave":"Arsenic|Trophic transfer|Food web|Lake Nahuel Huapi|Volcanic eruptions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3711,"idioma_id":4,"titulo":"Immunohistochemical analysis of the hypothalamic-pituitary-adrenal axis in dogs: sex-linked and seasonal variation","resumen":"This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ER&#945;).  An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ER&#945; and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ER&#945; in corticotrophs. Females had a greater proportion of corticotrophs per field (p < 0.01), with a greater cellular area and optical density (p < 0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p < 0.001). In zona fasciculata, ER&#945; and MC2R expression was greater in females (p < 0.001) and was greater in winter (p < 0.001). ER&#945; was identified in corticotrophs.  This study is the first to demonstrate ER&#945; expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations.","palabras_clave":"OESTROGEN RECEPTOR|MELANOCORTIN 2 RECEPTOR (MC2R)|SEXUAL DIMORPHISM|CORTICOTROPH ADRENAL CORTEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3712,"idioma_id":4,"titulo":"On cubic Kummer type towers of Garcia, Stichtenoth and Thomas","resumen":"In this paper we study the structure of a class of cubic tame towers having finite ramification locus. These towers were defined by Garcia, Stichtenoth and Thomas in 1997. We also prove some extensions of a well known result of H. Lenstra Jr. on the infiniteness of the ramification locus of these towers.","palabras_clave":"Function fi\felds, |Towers, |Genus, |Asymptotic behavior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3713,"idioma_id":4,"titulo":"Biosocial correlates and spatial distribution of consanguinity in South America.","resumen":"OBJECTIVE:To analyze potential biosocial factors in consanguineous unions according to the level of consanguinity and its spatial distribution in South America.METHODS:The data used came from the Latin American Collaborative Study of Congenital Malformations. Information on 126,213 nonmalformed newborns out of 6,014,749 births was used. This information was collected between 1967 and 2011 at 204 hospitals in 116 cities in 10 South American countries. The spatial scan statistic was performed under a model of nonhierarchical k-means segmentation, based on statistically significant clusters, areas with levels of high, medium, and low consanguinity were determined.RESULTS:Consanguinity in South America is heterogeneously distributed, with two groups of high consanguinity, in northwestern Venezuela and southeast of Brazil, and two clusters of low consanguinity located in the south of the continent, mainly Argentina. The socio-demographic factors associated with consanguinity influence the population structure in areas of high consanguinity.CONCLUSIONS:This study demonstrates that consanguinity in the South American continent is strongly associated with a greater magnitude of poverty in the area of high consanguinity.","palabras_clave":"Consanguinity|South America|Biosocial factor|Spatial distribution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3714,"idioma_id":4,"titulo":"Optimization of high hydrostatic pressure processing for the preservation of minimally processed peach pieces","resumen":"Consumers demand fresh-cut fruits, free from additives and withfresh appearance. However, the alteration caused by the tissue processinglimits their shelf life. The aim of this work was to optimize thepressure level (from 400 to 600 MPa) and the holding time (from 1 to 9min) of the High Pressure Processing (HPP) to achieve enzyme inactivationwhile preserving texture and color of minimally processed peaches. Peachcylinders were processed by combining dipping in organic acid solution,with vacuum-packaging and HPP at room temperature. Results showed thathigher pressure levels were more effective to inactivate enzymes and topreserve color than longer times. In addition, long treatments affectedthe microstructure and the texture of the tissues more seriously.Finally, a desirability study and a Principal Component Analysis wereperformed. These showed that the optimal treatment would be 585 MPa and 1min and that the best treatment of the ones studied was 600 MPa for 5min.","palabras_clave":"duraznos|alta presion|vacio|textura"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3715,"idioma_id":4,"titulo":"Resistance of foodborne pathogen coliphages to additives   applied in food manufacture","resumen":"In order to evaluate whether coliphages can be used in combination with food additives, six lytic phages against pathogenic Escherichia coli strains were tested for their resistance to additives commonly used in the dairy and meat industries. All the phages evaluated were completely inactivated after a 1-min incubation at 25°C when exposed to acetic and lactic acids at 4% v\/v without pH adjustment, whereas phage viability remained unchanged when pH was adjusted to 5.0 (acetic) and 4.5 (lactic). Likewise, the six phages proved to be highly resistant to both acetate and lactate (4%; sodium salts) after a 24-h incubation. When phage viability was evaluated at 25°C in Tris-Magnesium-Gelatin buffer supplemented with nitrite (0.015% w\/v), phage titers were never below 7-8 log PFU\/ml for all the phages tested. Regarding the influence of additives added to dairy products on phage viability, each phage (10exp7-8 PFU\/ml) challenged with nisin (0.25 mg\/ml) remained viable after a 24 h-incubation. In addition, phage viability was either slightly affected or not affected at all when phages were exposed to chymosin. These results proved that phages can be used against pathogenic E. coli strains along with other additives as an additional hurdle in order to improve food safety.","palabras_clave":"BACTERIOPHAGE|PHAGE VIABILITY|FOOD ADDITIVE|ESCHERICHIA COLI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3716,"idioma_id":4,"titulo":"Adulteration of Argentinean milk fats with animal fats: Detection by fatty acids analysis and multivariate regression techniques","resumen":"The adulteration of milk fat with foreing fats has been and still is a major concern in the dairy industry. The aims of the present study were to test the accuracy of the fatty acid relations established by the Argentinean Legislation to detect adulterations of milk fat with animal fats and to propose a regression model suitable to evaluate these adulterations, employing fatty acids profiles. For this purpose, 70 milk fat, 10 tallow and 7 lard samples were collected and analyzed by gas chromatography. Data was utilized to simulate arithmetically adulterated milk fat samples at 0, 2, 5, 10 and 15%, for both animal fats. The relations failed to distinguish adulterated milk fats containing less than 15% of tallow or lard. For each adulterant, a model was built and validated. Calibration and validation matrices were constructed employing genuine and adulterated milk fat samples selected by chance. For tallow and lard, the root mean square error of prediction (RMSP) values were 2.80% and 1.77% respectively. We found that the multivariate evaluation of fatty acids profile detected adulterated milk at levels greater than 5% for lard and 10% for tallow.","palabras_clave":"ADULTERATION|MILK FAT|MULTIPLE LINEAR REGRESSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3717,"idioma_id":4,"titulo":"Maternal separation in early life modifies anxious behavior and Fos and glucocorticoid receptor expression in limbic neurons after chronic stress in rats: effects of tianeptine","resumen":"Early-life adversity can lead to long-term consequence persisting into adulthood. Here, we assess the implications of an adverse early environment on vulnerability to stress during adulthood. We hypothesized that the interplay between early and late stress would result in a differential phenotype regarding the number of neurons immunoreactive for glucocorticoid receptor (GR-ir) and neuronal activity as assessed by Fos immunoreactivity (Fos-ir) in brain areas related to stress responses and anxiety-like behavior. We also expected that the antidepressant tianeptine could correct some of the alterations induced in our model. Male Wistar rats were subjected to daily maternal separation (MS) for 4.5 h during the first 3 weeks of life. As adults, the rats were exposed to chronic stress for 24 d and they were treated daily with tianeptine (10 mg\/kg intraperitoneal) or vehicle (isotonic saline). Fos-ir was increased by MS in all structures analyzed. Chronic stress reduced Fos-ir in the hippocampus, but increased it in the paraventricular nucleus. Furthermore, chronic stress increased GR-ir in hippocampus (CA1) and amygdala in control non-MS rats. By contrast, when MS and chronic stress were combined, GR-ir was decreased in these structures. Additionally, whereas tianeptine did not affect Fos-ir, it regulated GR-ir in a region-dependent manner, in hippocampus and amygdala opposing in some cases the stress or MS effects. Furthermore, tianeptine reversed the MS- or stress-induced anxious behavior. The interplay between MS and chronic stress observed indicates that MS rats have a modified phenotype, which is expressed when they are challenged by stress in later life.","palabras_clave":"AMYGDALA|ANTIDEPRESSANT|ELEVATED PLUS MAZE|FOS IMMUNOREACTIVITY|GLUCOCORTICOID RECEPTOR|HIPPOCAMPUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3718,"idioma_id":5,"titulo":"La construcción del espacio web de un instituto de investigación desde el  abordaje socio-técnico","resumen":"Se presentan aspectos significativos del proceso de construcción del espacio institucional web de un instituto de investigación en el campo de las Ciencias de la Educación. El marco teórico-metodológico del desarrollo se fundamentó en la perspectiva de los Dispositivos Hipermediales Dinámicos, atendiendo al despliegue del trabajo en redes colaborativas, las condiciones de inclusión social, el acceso abierto y la implementación de tecnologías de código abierto. El abordaje socio-técnico&nbsp; permitió una co-construcción organizacional y tecnológica acorde al actual contexto físico-virtual académico y de vinculación tecnológica y social. El alcance de lo propuesto podría ser orientador para otros centros, dado que propone una arquitectura virtual que integra el trabajo académico en red, la navegación accesible y lo dinámico de la gestión institucional.","palabras_clave":"DISPOSITIVO HIPERMEDIAL DINÁMICO|ACCESO ABIERTO|CONTEXTO FÍSICO-VIRTUAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3719,"idioma_id":4,"titulo":"The use of zeta potential as a tool to study phase transitions in binary phosphatidylcholines mixtures","resumen":"tTemperature dependence of the zeta potential (ZP) is proposed as a tool to analyze the thermotropicbehavior of unilamellar liposomes prepared from binary mixtures of phosphatidylcholines in the absenceor presence of ions in aqueous suspensions. Since the lipid phase transition influences the surface poten-tial of the liposome reflecting a sharp change in the ZP during the transition, it is proposed as a screeningmethod for transition temperatures in complex systems, given its high sensitivity and small amount ofsample required, that is, 70% less than that required in the use of conventional calorimeters. The sensitivityis also reflected in the pre-transition detection in the presence of ions. Plots of phase boundaries for thesemixed-lipid vesicles were constructed by plotting the delimiting temperatures of both main phase tran-sition and pre-transition vs. the lipid composition of the vesicle. Differential scanning calorimetry (DSC)studies, although subject to uncertainties in interpretation due to broad bands in lipid mixtures, allowedthe validation of the temperature dependence of the ZP method for determining the phase transition andpre-transition temperatures. The system chosen was dipalmitoylphosphatidylcholine\/dimyristoyl phos-phatidylcholine (DMPC\/DPPC), the most common combination in biological membranes. This work maybe considered as a starting point for further research into more complex lipid mixtures with functionalbiological importance.","palabras_clave":"LIPID MIXTURES|UNILAMELLAR VESICLES|ZETA POTENTIAL|TRANSITION TEMPERATURES|IONIC MEDIUM|PLOT OF PHASE BOUNDARIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3720,"idioma_id":4,"titulo":"Water erosion and connectivity analysis during a year with high precipitations in a watershed of Argentina","resumen":"Soil erosion is a global concern because of its consequences for the environment and the economy of countries. In the Argentine Pampas Region soil erosion process is a priority issue, although there is little information about sediment concentration in agricultural catchments. The study aimed at assessing the factors that have a major influence on sediment concentration (SC) and discussing the dynamics of hydrological and sedimentological connectivity during 2012, a year with precipitation over the mean and significant erosive events. The study was conducted in a watershed of Buenos Aires province, Argentina. A linear regression model, that considered autocorrelation, was obtained. Maximum rainfall intensity in 30 minutes and peak flow were related to SC. An analysis of satellite images was carried out to discuss the hydrological connectivity, and a connectivity index was calculated to assess changes in sedimentological connectivity. The analyses suggested increments in hydrological and sedimentological connectivity, associated with the drainage area expansion and with water erosion rills. Hydrological connectivity is needed for sedimentological connectivity. However, increments in sedimentological connectivity may have been conditioned by the input of energy to detach and to transport particles. This may have been evidenced when flows exceeded a threshold runoff coefficient.","palabras_clave":"HYDROLOGICAL CONNECTIVITY|RILL EROSION|RUNOFF|SEDIMENT CONCENTRATION|SEDIMENTOLOGICAL CONNECTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3721,"idioma_id":5,"titulo":"Estar cerca de Europa. Deporte, clase social y prestigio en Argentina","resumen":"El siguiente artículo propone pensar la clase social vinculada al deporte como espacio de sociabilidad y de distinción en la Argentina. A través de la idea de que las clases sociales se constituyen y se piensan, analíticamente, en movimiento, se indaga cómo sujetos que practican rugby, en La Plata, otorgan sentido a su posición social, económica, política y cultural en relación directa al deporte recreado colectivamente por sectores dominantes en la ciudad. El rugby, en Argentina, es un deporte históricamente concebido y practicado por grupos minoritarios, no es una actividad de participación masiva. Se analizará cómo las influencias sociales y culturales que, desde Francia e Inglaterra, modelaron ?y modelan- una forma particular de percibir el mundo entre hombres que juegan rugby en Argentina, excede al campo deportivo. El desafío de este trabajo es comprender la dinámica de estos espacios, desde la dimensión cultural de la clase social, en tanto experiencia que organiza las prácticas, ampliando la restringida definición económica.&nbsp;","palabras_clave":"Rugby|Sectores Dominantes|Distinción|Sociabilidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3723,"idioma_id":4,"titulo":"Rediscovery and lectotypification of Philonotis brevifolia Herzog (Bartramiaceae, Bryophyta), a neglected species from Chile","resumen":"Jimenez, S., G. Suárez & J. Larraín. 2016. Rediscovery and lectotypification ofPhilonotis brevifolia Herzog (Bartramiaceae, Bryophyta), a neglected speciesfrom Chile. Cryptogamie, Bryologie 37(2): 113?118.","palabras_clave":"CHILE|PERIGONIAL LEAVES|PHILONOTIS KRAUSEI|TAXONOMY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3724,"idioma_id":4,"titulo":"Selective pressure against horizontally acquired prokaryotic genes as a driving force of plastid evolution","resumen":"The plastid organelle comprises a high proportion of nucleus-encoded proteins that wereacquired from different prokaryotic donors via independent horizontal gene transfersfollowing its primary endosymbiotic origin. What forces drove the targeting of these alienproteins to the plastid remains an unresolved evolutionary question. To better understand thisprocess we screened for suitable candidate proteins to recapitulate their prokaryote-toeukaryotetransition. Here we identify the ancient horizontal transfer of a bacterial polyphenoloxidase (PPO) gene to the nuclear genome of an early land plant ancestor and infer thepossible mechanism behind the plastidial localization of the encoded enzyme. Arabidopsisplants expressing PPO versions either lacking or harbouring a plastid-targeting signal allowedexamining fitness consequences associated with its subcellular localization. Markedly, adeleterious effect on plant growth was highly correlated with PPO activity only whenproducing the non-targeted enzyme, suggesting that selection favoured the fixation of plastidtargetedprotein versions. Our results reveal a possible evolutionary mechanism of howselection against heterologous genes encoding cytosolic proteins contributed in incrementingplastid proteome complexity from non-endosymbiotic gene sources, a process that may alsoimpact mitochondrial evolution.","palabras_clave":"horizontal transfer|evolution|plant|plastid"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3725,"idioma_id":5,"titulo":"La problemática del arsénico en el servicio de agua en la provincia de Buenos Aires, Argentina","resumen":"En Argentina la problemática del acceso al agua conforma un tema que se ha insertado en la agenda pública a partir de, por un lado la creciente contaminación del recurso hídrico y su mercantilización y por el otro, la judicialización de distintos conflictos por el acceso y calidad del agua. Las empresas proveedoras del servicio enfrentan un conjunto de desafíos vinculados a la necesidad de sostener y mejorar la provisión en el territorio e incluir a nuevos usuarios. Puntualmente en una parte importante de la Provincia de Buenos Aires (PBA), la empresa Aguas Bonaerense S.A (ABSA) y otros organismos municipales que proveen el servicio de agua enfrentan serios cuestionamientos judiciales vinculados a los altos valores de arsénico y nitratos presentes en el agua que provee a los hogares.El objetivo general del presente artículo es analizar una selección de casos jurisprudenciales vinculados con la calidad del agua potable que se consume en el ámbito de la PBA.Luego de la presentación de los objetivos y metodología adoptada se caracteriza el territorio de la PBA y los municipios afectados por los fallos (Lincoln, 9 de Julio, Carlos Casares y Junín); posteriormente, se estudia el diseño institucional y regulatorio del servicio de agua; seguido se analizan los fallos respecto a los altos niveles de arsénico presentes en el agua que se distribuye en los municipios señalados; y por último, a modo de cierre, sus potenciales implicancias respecto a otros procesos judiciales en curso en la PBA.","palabras_clave":"DERECHO AMBIENTAL|REGULACIÓN|FALLOS JUDICIALES|ARSÉNICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3726,"idioma_id":4,"titulo":"Insect Larvae: A New Platform to Produce Commercial Recombinant  Proteins","resumen":"Abstract: In Biotechnology, the expression of recombinant proteins is a constantly growing field and different hosts are used for this purpose. Some valuable proteins cannot be produced using traditional systems. Insects from the order Lepidoptera infected with recombinant baculovirus have appeared as a good choice to express high levels of proteins, especially those with post-translational modifications.Lepidopteran insects, which are extensively distributed in the world, can be used as small protein factories, the new biofactories. Species like Bombyx mori (silkworm) have been analyzed in Asian countries to produce a great number of recombinant proteins for use in basic and applied science and industry. Many proteins expressed in this larva have been commercialized. Several recombinant proteins produced in silkworms have already been commercialized. On the otherhand, species like Spodoptera frugiperda, Heliothis virescens, Rachiplusia nu, Helicoverpa zea and Trichoplusia ni are widely distributed in both the occidental world and Europe. The expression of recombinant proteins in larvae has the advantage of its low cost in comparison with insect cell cultures. A wide variety of recombinant proteins, including enzymes, hormones and vaccines, have been efficiently expressed with intact biological activity. The expression of pharmaceutically proteins, using insect larvae or cocoons, has become very attractive. This review describes the use of insect larvae as an alternative to produce commercial recombinant proteins.","palabras_clave":"INSECT LARVAE|BIOFACTORIES|RECOMBINANT PROTEINS|BACULOVIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3727,"idioma_id":4,"titulo":"Lead and zinc determinations in Festuca arundinacea and Cynodon dactylon collected from contaminated soils in Tandil (Buenos Aires Province, Argentina)","resumen":"Abstract Heavy metal soil contamination is one of themost serious environmental problems, considering boththeir persistence and progressive accumulation whichmakes possible the transference to other systems, and couldpotentially affect human health and ecosystems functioning.The total concentration of lead (Pb) and zinc (Zn) insoil and plant samples from Festuca arundinacea andCynodon dactylon naturally developed (in situ) and withinexperimentally contaminated soils (ex situ) was measured.Pb and Zn obtained results showed that the average valuesin industrial soils were significantly higher (p\\0.0001)than those corresponding to controls values (472 % morefor Pb and 288 % more for Zn). There was a positivesignificant correlation between Pb levels in soil and roots(r = 0.99) and leaves (r = 0.98) of C. dactylon, andbetween Zn levels in soil and roots (r = 0.94) and leaves(r = 0.91) of C. dactylon. Festuca arundinacea plantswere experimentally exposed to Pb-contaminated soilduring different times (0, 5 and 10 days). Two indicatorswere calculated: the bioaccumulation factor (BCF) andtranslocation factor (TF). Five day BCF was 0.25, while for10 days one was 0.72. This showed that the value of BCFvaried according to the exposure time, and F. arundinaceashowed to be highly tolerant to Pb species. TF was low (T1:0.18 and T2: 0.09) because the higher accumulationoccurred in roots. Considering that roots of F. arundinaceaand C. dactylon were their largest metals reservoir, theycould be used as indicators of metal contamination withinsoils","palabras_clave":"Lead|Zinc|Festuca arundinacea|Cynodon dactylon"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3728,"idioma_id":5,"titulo":"Determinación de la Respuesta Dinámica y Estacionaria de la Frecuencia frente a Desbalances de Potencia Producidos por Perturbaciones en la Red","resumen":"This paper proposes a new methodology for determining the transient and steady state frequency deviations produced by disturbances in the power system that modify the generation-load balance. Based on the equivalent frequency response model, analytical expressions are derived to calculate the maximum transient frequency deviation, the instant it occurs, and the amount of load to be shed due to Under Frequency Load Shedding Scheme (UFLS) action. To this end, a first order model is employed to represent the governor-turbine block of each generator. In this paper, the model has been extended to represent hydro turbine speed governors of different technology. Additionally, the methodology determines the post-disturbance steady state frequency, after primary frequency control (PFC), taking into consideration the load disconnected by UFLS and spinning reserve limits of generators under PFC. Lastly, the proposed methodology is applied to two test systems, showing an excellent ability to accurately estimate the transient and steady state frequency response, due to active power imbalances.","palabras_clave":"FREQUENCY RESPONSE MODEL|TRANSIENT RESPONSE|POST-DISTURBANCE FREQUENCY|PRIMARY FREQUENCY CONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3729,"idioma_id":4,"titulo":"Isolation of atypical enteropathogenic Escherichia coli from chicken and chicken-derived products","resumen":"1. Atypical enteropathogenic Escherichia coli strains from chicken and chickenderivedproducts were isolated and characterised.2. The strains presented a wide variety of serotypes, some have been reported in other animalspecies (O2:H40, O5:H40) and in children with diarrhoea (O8:H-). Most of the strains carriedintimin &#946;.3. The results indicate that chicken and chicken products are important sources of atypicalenteropathogenic E. coli strains that could be associated with human disease and highlightsthe need to improve hygiene practices in chicken slaughtering and meat handling.","palabras_clave":"Serotypes|EPEC|chicken|diarrhea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3730,"idioma_id":4,"titulo":"Expression of grapevine AINTEGUMENTA-like genes is associated with variation in ovary and berry size","resumen":"Fruit size is a highly important trait for most fruit and vegetable crops. This trait has been a main selection target and could be involved in divergent selection processes leading to the differentiation between modern table and wine cultivars. Even though its determination is highly influenced by cultural practices, several regions within the grapevine genome have been identified influencing berry size, mainly those related to seedlessness, but also particular transcription factors affecting cell number and\/or cell size of flowers or berries. In this sense, we analyzed the relationship between the cell number of ovaries from different grapevine cultivars and final size of the flowers and berries of those cultivars. We also performed the characterization of the grapevine AINTEGUMENTA-LIKE family, particularly focused in AINTEGUMENTA (ANT), since it is well reported in Arabidopsis that ANT regulates cell proliferation and organ growth by maintaining the meristematic competence of cells during organogenesis. The present work showed the existence of a clear association between the berry and ovary size, and that the ovary cell number is a main determinant of ovary size. Our results also suggest the putative involvement of ANT in the determination of those important grapevine quality traits.","palabras_clave":"BERRY SIZE|CELL DIVISION|GENE EXPRESSION|OVARY SIZE|VITIS VINIFERA|AINTEGUMENTA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3731,"idioma_id":4,"titulo":"Isolation of Shiga toxin-producing Escherichia coli from ground beef using multiple combinations of enrichment broths and selective agars","resumen":"Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens, and beef cattle are recognized as the principal reservoir. The aims of this study were: 1. to identify the most sensitive combination of selective enrichment broths and agars for STEC isolation in artificially inoculated ground beef samples, and 2. to evaluate the most efficient combination(s) of methods for naturally contaminated ground beef samples. A total of 192 ground beef samples were artificially inoculated with STEC and non-stx bacterial strains. A combination of four enrichment broths and three agars were evaluated for sensitivity, specificity, and positive predictive value for STEC isolation from experimentally inoculated samples. Enrichments with either modified tryptic soy broth (mTSB) containing 8 mg L-1 novobiocin (mTSB-8) or modified Escherichia coli (mEC) broth followed by isolation in MacConkey agar were the most sensitive combinations for STEC isolation of artificially inoculated samples. Independently, both enrichments media followed by isolation in MacConkey were used to evaluate ground beef samples from 43 retail stores, yielding 65.1% and 58.1% stx-positive samples by RT-PCR, respectively. No difference were observed in the isolate proportions between these two methods (8\/25, 32% and 8\/28, 28.6%). Identical serotypes and stx genotypes were observed in STEC strains isolated from the same samples by either methods. In this study, no single enrichment protocol was sufficient to detect all STEC in artificially inoculated samples, and had considerable variation in detection ability with naturally contaminated samples. Moreover the usage of single or combination of multiple isolation agars were not capable of identifying all STEC serogroups in either artificially inoculated or naturally occurring STEC-contaminated ground beef. Therefore, it may be prudent to conclude that there is no single method or combination of isolation methods capable of identifying all STEC serogroups.","palabras_clave":"ENRICHMENT|GROUND BEEF|ISOLATION|SHIGA TOXIN-PRODUCING ESCHERICHIA COLI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3732,"idioma_id":4,"titulo":"Spherulitic growth and crystallization kinetics in PHB\/DGEBA blends","resumen":"A study of spherulitic growth in PHB and PHB\/DGEBA (poly(3-hydroxybutyrate)\/diglycidyl ether ofbisphenol A) blends with PHB\/DGEBA weight ratios of 100\/0, 90\/10, 80\/20, 70\/30 and 60\/40 crystallizedat temperatures in the range of 45?110&#9702;C is carried out. The spherulitic growth rates at the crystallizationtemperatures are studied by optical microscopy. Differential scanning calorimetry is used to study thesame isothermal process to fit the results by means of the Avrami theory to determine the characteristicparameters of the process. The obtained characteristic parameters are compared to the spherulitic growthrates that are determined by optical microscopy. The observed differences are discussed considering theprocess of nucleation. An increase in the secondary crystallization process is observed with increasingcrystallization temperature and DGEBA content in the blend. Changes in the crystallization associatedwith an increase in the rigid amorphous fraction (RAF) mobility are discussed.","palabras_clave":"Crystallization|Blends|Poly(3-hydroxybutyrate)|Epoxy Resin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3733,"idioma_id":4,"titulo":"Antioxidant effect of modified bovine plasma proteins via Maillard reaction on n-3 fortified beef patties","resumen":"This work aimed to develop a healthier meat product, by improving nutritional properties related to the lipid profile, using a Maillard reaction product (MRP) obtained from recycling blood from local slaughterhouses as antioxidant. Soybean oil was used as partial fat replacer and the lipid profile was modified as a decrease in the n6\/n3 ratio (11.59 to 9.50), atherogenic and trombogenic index (from 0.42 to 0.27 and 0.94 to 0.56 respectively) was observed. On the basis of previous studies, beef patties were added with two MRP levels (1% w\/w and 3% w\/w). Patties with no antioxidants were used as control and patties containing 0.01% w\/w of butylhydroxyanisole (BHA) were used as reference. Oxidative stability of refrigerated vacuum ? packed patties was evaluated during 15 days of storage. Results showed 3% of MRP as the most appropriate concentration to prevent lipid oxidation, with inhibition percentages of 75%, and 9 days as the limit of chilled storage regarding consumers &#769;","palabras_clave":"MEAT INDUSTRY WASTE|NATURAL OCCURRING ANTIOXIDANT|LIPID OXIDATION|FUNCTIONAL MEAT PRODUCTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3734,"idioma_id":4,"titulo":"Length-weight relationships of 29 species teleosts fish from shallow estuarine environment of the Samborombón bay (Río de la Plata, Argentina)","resumen":"The length?weight relationships (LWR) of 29 teleost fish species from the shallow estuarine environments of the Samborombón Bay wetland (Río de la Plata, Argentina) were estimated. A total of 6,376 individuals belonging to 19 families were measured and weighed. The best represented family was Sciaenidae with 6 species, followed by Clupeidae (n = 3), and Heptapteridae and Characidae with 2 species each. Significant length?weight relationships with high correlation coefficients were found for all species, which mostly presented positive allometric growth. This study reports the first findings regarding the LWR of 29 species in the Samborombón Bay waters. New maximum sizes and geographic distribution records of certain species are also commented.","palabras_clave":"Length-weight |Samborombón Bay |Fish"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3735,"idioma_id":4,"titulo":"Effectiveness of fencing and hunting to control Lama guanicoe browsing damage: Implications for Nothofagus pumilio regeneration in harvested forests","resumen":"Browsing damage by native ungulates is often to be considered one of the reasons of regeneration failure in Nothofagus pumiliosilvicultural systems. Fencing and hunting in forests at regeneration phase have been proposed to mitigate browsing effects. This study aims to determine effectiveness of these control methods in harvested forests, evaluating browsing damage over regeneration, as well as climate-related constraints (freezing or desiccation). Forest structure and regeneration plots were established in two exclosures against native ungulates (Lama guanicoe) by wire fences in the Chilean portion of Tierra del Fuego island, where tree regeneration density, growth, abiotic damage and quality (multi-stems and base\/stem deformation) were assessed. Exclosures did not influence regeneration density (at the initial stage with1.3 m high). However, sapling height at 10-years old was significantly lower outside (40e50 cm high) than inside exclosures (80e100 cm), and also increased their annual height growth, probably as a hunting effect. Likewise, quality was better inside exclosures. Alongside browsing, abiotic conditions negatively influenced sapling quality in the regen-eration phase (20%e28% of all seedlings), but greatly to taller plants (as those from inside exclosure). This highlights the importance of considering climatic factors when analysing browsing effects. For best re-sults, control of guanaco in recently harvested areas by fencing should be applied in combination with a reduction of guanaco density through continuous hunting. The benefits of mitigation actions (fencing and hunting) on regeneration growth may shorten the regeneration phase period in shelterwood cutting forests (30e50% less time), but incremental costs must be analysed in the framework of management planning by means of long-term studies.","palabras_clave":"browsing|exclosures|regeneration growth|plant quality"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3736,"idioma_id":4,"titulo":"Functional evaluation of ToxA promoter in Trichoderma reesei Rut C30","resumen":"In order to expand the collection of promoters studied in Trichodermareesei Rut-C30, a functional study of the ToxA promoter was carried out byanalysis of GFP expression in T. reesei Rut-C30. For this purpose, thebinary pCBCT expression vector was employed to transform T. reesei Rut-C30. The transformants obtained were evaluated by means of fluorescencemicroscopy, fluorometry, dot blot and Western blot analysis. The low levelsof cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoterworks as a weak constitutive promoter which can drive successfully theexpression of heterologous proteins in T. reesei Rut-C30","palabras_clave":"TRICHODERMA|WEAK PROMOTER|RUT C-30"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3737,"idioma_id":4,"titulo":"An economical and high throughput alternative for endoglucanase activity determination","resumen":"The search for new strains with good productivity of cellulolytic enzymesis always necessary to improve the processes are usually employed.Screening protocols used for these purposes require a low cost and highthroughput assay for determination of endoglucanase activity. However,the test proposed by the IUPAC and other microadaptations not meet thisrequirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanaseactivity assay adaptation. For this we reduced the assay volume andinvestigate the effect of this reduction on endoglucanase activitydetermination. As a result a miniaturized assay statistically correlated withthe assay proposed by IUPAC reduced 10-fold lower scale was obtained.As a result a new economic and high performance adaptation forendoglucanase activity assay based on the IUPAC protocol was obtained","palabras_clave":"Miniaturized CMCase assay|Cellulase|Endoglucanase|Carboxymethylcellulases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3738,"idioma_id":4,"titulo":"Combinatorial library screening coupled to mass spectrometry to identify valuable cyclic peptides","resumen":"Combinatorial library screening coupled to mass spectrometry (MS) analysis has proven to be a practical approach to identify useful peptides. Cyclic peptides usually have high biological activity, selectivity and affinity for target proteins, and strong stability against proteolytic degradation. Here, we describe two strategies to prepare combinatorial libraries suitable for MS analysis to accelerate the discovery of cyclic peptides structures. In both approaches ChemMatrix resin and the linker 4-hydroxymethylbenzoic acid is used. In one strategy, a one-bead-two-peptides library in which each bead contains both the cyclic peptide and its linear counterpart, facilitates MS analysis. The other protocol is based on the synthesis of a cyclic depsipeptide library in which a glycolamidic ester group is incorporated by adding glycolic acid. After library screening, the ring is opened and the peptide is released simultaneously for subsequent MS analysis.","palabras_clave":"DEPSIPEPTIDE|COMBINATORIAL|AFFINITY|MALDI MS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3739,"idioma_id":4,"titulo":"Latest advances in OBOC peptide libraries. Improvements in screening  strategies and enlarging the family from linear to cyclic libraries","resumen":"Abstract: Solid phase screenings of one bead one compound(OBOC) libraries have been widely used to find ligandswith pharmacological and analytical uses, and to purify or detect proteins incomplex mixtures. To improve library screening, in the last years variousstrategies have been developed to avoid the selection of false positive beadsand to obtain selective ligands. Currently,there is great interest in cyclic peptides because of their resistance toenzymatic degradation and higher selectivity compared to their linearcounterparts. Lots of cyclic peptide libraries protocols have been recentlydeveloped to facilitate hits analysis. The aim of this review is to summarizethe latest applications of solid phase screening of OBOC combinatorial peptidelibraries, the improvements in the screening methods including massspectrometry MS\/MS techniques, and the strategies to synthesize OBOC cyclicpeptide libraries.","palabras_clave":"MASS SPECTROMETRY|TANDEM MS\/MS|PEPTIDE|PEPTOID|CYCLIC|LIBRARY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3740,"idioma_id":5,"titulo":"Impacto de la envolvente en la demanda de energía en calefacción residencial de la región metropolitana de  La Plata , tomando como caso testigo el reciclado energético de una vivienda.","resumen":"El área metropolitana de La Plata representa el 1,6% de las viviendas dela Argentina. A partir del análisis del reciclado de una vivienda se construyenindicadores básicos con el fin de proponer una metodología que facilite laextrapolación, a nivel regional, del impacto que causaría un programa deeficiencia energética en viviendas. Se exponen desde el nivel micro a macro losresultados obtenidos en un contexto de carestía energética y recurrentes crisisen la oferta de combustibles y electricidad en la Argentina. La mejora en la resistenciatérmica de muros y techos en todo el parque residencial de La Plata conllevaríaa una reducción del 12% de la energía consumida en calefacción, y un ahorro de30.000 TEP\/año. Se concluye que la incidencia que implica la colocación deaislación adicional respecto del costo de la construcción justifica su uso,resolviendo parcialmente la falta de combustibles en el país, reduciendo gastosfijos en la vivienda, otorgando confort térmico a los usuarios y generandoreactivación del sector de la construcción.","palabras_clave":"Eficiencia Energética|Confort térmico|EIFS|AuditCad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3741,"idioma_id":4,"titulo":"Direct Detection of Double Retrograde Behavior in Binary Systems  for Equation Of State Models","resumen":"In this work, a straightforward methodology is proposed to establish at a glance, for binary systems, whether a model of the equation of state type predicts the occurrence of double retrograde behavior (DRB), and to also establish what the ranges of conditions of DRB existence are. This is accomplished by computing three hyper-lines, which usually are highly non linear. One of them is the vapor-liquid critical line. The other two are loci of cricondenbar points and of cricondentherm points. Each line is efficiently calculated in a single computer run by resorting to a robust numerical continuation method.","palabras_clave":"double retrograde behavior|equation of state|local extrema|phase diagram"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3742,"idioma_id":4,"titulo":"Azadinium poporum from the Argentine Continental Shelf, Southwestern Atlantic, produces azaspiracid-2 and azaspiracid-2 phosphate","resumen":"The marine dinophycean genus Azadinium has been identified as the primary source of azaspiracids (AZA), a group of lipophilic phycotoxins known to accumulate in shellfish. Blooms of Azadinium in the southern Atlantic off Argentina have been described from the 1990s, but due to a lack of cultures, the diversity of South-Atlantic Azadinium has not yet been fully explored and their toxin production potential is completely unknown. During a spring 2010 research cruise covering the El Rincon (ER)estuarine system (North Patagonian coast, Argentina, Southwestern Atlantic) a search was conducted for the presence of Azadinium. Although neither Azadinium cells nor AZA in field plankton samples weredetected, 10 clonal strains of Azadinium poporum were successfuly established by incubation of sediment samples. Argentinean A. poporum were more variable in size and shape than the type description butconformed to it by the presence of multiple pyrenoids with starch sheath, in plate pattern and arrangement, and in the position of the ventral pore located on the left side of the pore plate. In contrast to all previous description of A. poporum, isolates of the Argentinean A. poporum possessed a distinct field of pores on the second antapical plate. Conspecificity of the Argentinean isolates with A. poporum wasconfirmed by molecular phylogeny of concatenated ITS and LSU rDNA sequences, where all Argentinean isolates together with some Chinese A. poporum strains formed a well-supported ribotype clade within A.poporum. All isolates produced AZA with the same profile, consisting of AZA-2 as the major compound and, to a lesser extent, its phosphated form. This is the first report of a phosphated marine algal toxin.This first confirmation of the presence of AZA producing Azadinium in the Argentinean coastal area underlines the risk of AZA shellfish contamination episodes in the Southwestern Atlantic region.","palabras_clave":"AZADINIUM|AZASPIRACIDS |SOUTHWESTERN ATLANTIC| ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3743,"idioma_id":4,"titulo":"Biological activities of polyphenols-enriched propolis from Argentina arid regions","resumen":"Background: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) fromplant sources.Purpose: This studywas undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity\/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions.Study design\/methods: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis were obtained. The PEEs were analyzed for total polyphenol(TP), non-flavonoid phenols (NFP) and flavonoid (FP) content followed by HPLC-DAD analysis and identification of components by HPLC-MS\/MSn. The potentiality as anti-inflammatory (LOX, COX, iNOS enzymes), antioxidant, antifungal and nematicidal was determined.Results: PEEs contain high levels of TP, NFP and FP, including cinnamic acid, caffeic acid prenyl ester, caffeoyl dihydrocaffeoate and caffeic acid 3,4-dihydroxyphenethyl ester, liquiritigenin, 2,4-dihydroxychalcone and 2,4-dihydroxy-3-methoxychalcone. The PEEs in vitro reduced the activity of LOX and COX-2. Pretreatment of RAW 264.7 cells with PEEs before the induction of inflammatory state, inhibited NO overproduction and the iNOS protein expression was significantly decreased. The PEEs exhibited antioxidant, antifungal (Candidasp.) and nematicidal effect (C. elegans).Conclusion: These findings show the potential use of characterized PEEs from arid regions of Argentina as phytomedicine.","palabras_clave":"Argentina|propolis|antihelmintic|anti-inflammatory|antifungal|polyphenols"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3744,"idioma_id":4,"titulo":"Antioxidant and anti-inflammatory activities of Frankenia triandra (J. Rémy) extracts","resumen":"Frankenia triandra (Frankeniaceae) is a poorly studied halophyte subshrub species that grows on saline soils inthe Bolivian, Argentinean and Chilean Puna, and is used by Puna inhabitants as forage and antiseptic in their folk medicine. The aim of this work is to first evaluate the antioxidant and anti-inflammatory potential (inhibitory effect on pro-inflammatory enzymes) of two hydroalcoholic extracts of F. triandra obtained by maceration and soxhlet extraction. Both extracts showed similar biological activity and composition (mainly polyphenolic compounds, flavonoids and phenolic acids). They exhibited an important antioxidant activity by scavenging ABTS?+ and nitrite radicals, by inhibiting &#946;-carotene bleaching and reducing Fe3+. Antioxidant properties are related to anti-inflammatory capacity, and both extracts showed a significant inhibition of hyaluronidase and two enzymes of arachidonic acid pathway (cyclooxygenase-2 and lipoxygenase). These preliminary studies are interesting since they might open the way for further studies which would allow the potential use of this plant in the treatment of chronic inflammatory diseases and as an antioxidant agent.","palabras_clave":"FRANKENIACEAE|ARGENTINE PUNE|PHYTOCHEMISTRY|CLYCLOOXYGENASE|LIPOXYGENASE|HYALURONIDASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3745,"idioma_id":4,"titulo":"EVALUATION OF ECOLOGICAL EFFECTS OF ANTHROPOGENIC NUTRIENT LOADING SCENARIOS IN LOS MOLINOS RESERVOIR THROUGH A MATHEMATICAL MODEL","resumen":"TRABAJO DESARROLLADO, ENVIADO Y ACEPTADO DURANTE EL AÑO 2015 (enviado en abril de 2015, aceptado en octubre 2015, tal como figura en la historia del artículo - ver adjunto en Sigeva -).In this paper we perform a scenario analysis to assess the likely ecosystem structural shifts that might be induced by seven alternative management scenarios relative to current simulated lake dynamics of Los Molinos reservoir, a monomictic lake located in Argentina. To carry out the scenario analysis, first, we formulate, calibrate and validate a mechanistic biogeochemical model to characterize the nutrients and phytoplankton dynamics of the reservoir under study. The model includes a set of partial differential algebraic equations, which results from dynamic mass balances on main phytoplankton groups, nutrients, dissolved oxygen and biochemical demand of oxygen. We consider the main sources of nutrient loading and the dominant phytoplankton groups. The parameter estimation model is a constrained dynamic optimization problem that has been formulated within an equation oriented control vector parameterization environment. We estimated sixteen parameters, previously identified as the most influential ones. Numerical results for calibration and validation phases show good agreement with field data, and then, we use the proposed model as a prospective tool. The obtained results for the designed nutrient loading scenarios indicate the importance of the impacts of current livestock practices and sewage\/collective septic tanks discharges on the water quality. Model predictions indicate that the combination of reduction in nutrient loading from the sewage of residential areas in the lake shore, and implementation of measures to mitigate nutrient exports from livestock production will render the most beneficial effects over the studied ecosystem, thus promoting the improvement of the current ecological state of the reservoir. This is the first time such an approach has been applied to the study of Los Molinos catchment. Thus, the proposed ecological model constitutes a management tool to assess the anthropogenic impacts over this freshwater resource.","palabras_clave":"Ecological model|Scenario development|Prospections|Dynamic parameter estimation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3746,"idioma_id":4,"titulo":"Hepatitis C virus strategies to evade the specific-T cell response: a possible mission favoring its persistence","resumen":"Hepatitis C virus (HCV) is a small, enveloped RNA virus. The number ofHCV-infected individuals worldwide is estimated to be approximately 200million. The vast majority of HCV infections persist, with up to 80% of allcases leading to chronic hepatitis associated with liver fibrosis, cirrhosis,and hepatocellular carcinoma. The interaction between HCV and the host have apivotal role in viral fitness, persistence, pathogenicity, and diseaseprogression. The control of HCV infection requires both effective innate andadaptive immune responses. The HCV clearance during acute infection isassociated with an early induction of the innate and a delayed initiation ofthe adaptive immune responses. However, in the vast majority of acute HCVinfections, these responses are overcome and the virus persistence almostinexorably occurs. Recently, several host- and virus-related mechanismsresponsible for the failure of both the innate and the adaptive immuneresponses have been recognized. Among the latter, the wide range of escapemutations to evade the specific-T-and B-cell responses as well as the T cellanergy and the CD8+ T cell exhaustion together with the interference with itsfunction after prolonged virus exposure hold a pivotal role. Other HCVstrategies include the modification or manipulation of molecules playing keyroles in the induction of the interferon response and its induced effectorproteins. In this review, we attempt to gain insights on the main T cell immuneevasion strategies used by the virus in order to favor its persistence.","palabras_clave":"HCV|Immune evasion|CD4+ T cell|CD8+ T cell"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3747,"idioma_id":4,"titulo":"Superparamagnetic iron-oxide nanoparticles mPEG350 ? and mPEG2000-coated: cell uptake and biocompatibility evaluation","resumen":"Superparamagnetic iron oxide nanoparticles (SPIONS) were synthesized by thermal decomposition of an organometallic precursor at high temperature and coated with a bi-layer composed of oleic acid and methoxy-polyethylene glycol-phospholipid. The formulations were named SPION-PEG350 and SPION-PEG2000. Transmission electron microscopy, X-ray diffraction and magnetic measurements show that the SPIONs are near-spherical, well-crystalline, and have high saturation magnetization and susceptibility. FTIR spectroscopy identifies the presence of oleic acid and of the conjugates mPEG for each sample. In vitro biocompatibility of SPIONS was investigated using three cell lines; up to 100 &#956;g\/ml SPION-PEG350 showed non-toxicity, while SPION-PEG2000 showed no signal of toxicity even up to 200 &#956;g\/ml. The uptake of SPIONS was detected using magnetization measurement, confocal and atomic force microscopy. SPION-PEG2000 presented the highest internalization capacity, which should be correlated with the mPEG chain size. The in vivo results suggested that SPION-PEG2000 administration in mice triggered liver and kidney injury.","palabras_clave":"Superparamagnetic iron-oxide nanoparticles (SPIONS);|Uptake|In vitro|In vivo|Biocompatibility"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3748,"idioma_id":4,"titulo":"HOLOTECH prototype: Sound Language for Environment's understanding","resumen":"The aim of this paper is to present a prototype to help blind people to move around avoiding environment's potential hazards using a specific sound language. Obstacles detection and its risk value are based on a combination of image processing, Haar like Feature-based Cascade Classifier and a ruled Expert System to feature and recognize objects in real-time. The lightweight algorithm that models objects in the nearby, provide quick identification of the images. Signals collected using a simple smart phone and two ultrasound sensors attached to an arduino nano by a case located on the chest of the individual. A special language based on sounds assists in the displacement of blind persons, telling them where are main obstacles and its position. The main contribution of this work is automate the selection model for proper combination of information, discarding unnecessary data and defining the minimum precision requirements to fulfill the current goal. This is important because many solutions in the field lack of precision for obstacles placed in short distances from the individual, are too slow, or not quick enough to notify them in advance. Others are expensive, or too heavy to be used comfortably in an all-day basis.  Authors present the state of the art, the model and prototype HOLOTECH that implements the proposal, along with statistical information and evaluation of the performance.","palabras_clave":"Computational Intelligence|Blind People|Expert Systems|Neural Networks"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3749,"idioma_id":4,"titulo":"Silhouette + Attraction: A Simple and Effective Method for Text Clustering","resumen":"This article presents silhouette?attraction (Sil?Att), a simple and effective method for text clustering, which is based on two main concepts: the silhouette coefficient and the idea of attraction. The combination of both principles allows us to obtain a general technique that can be used either as a boosting method, which improves results of other clustering algorithms, or as an independent clustering algorithm. The experimental work shows that Sil?Att is able to obtain high-quality results on text corpora with very different characteristics. Furthermore, its stable performance on all the considered corpora is indicative that it is a very robust method. This is a very interesting positive aspect of Sil?Att with respect to the other algorithms used in the experiments, whose performances heavily depend on specific characteristics of the corpora being considered.","palabras_clave":"TEXT CLUSTERING|SILHOUETTE COEFFICIENT|ATTRACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3750,"idioma_id":5,"titulo":"Caracterización de la estructura de los rebaños ovinos del norte de la provincia de La Pampa","resumen":" En la zona central de la República Argentina se encuentra la provincia de La Pampa. El norte de la misma se caracteriza por ser agrícola-ganadero, con poblaciones ovinas de biotipos heterogéneos, utilizadas para auto-abasto y comercialización de corderos. El objetivo de este estudio fue caracterizar los rebaños ovinos de la región según denominación racial y estructura poblacional. Durante los años 2012-2013 se realizó un relevamiento poblacional de 8 rebaños. Se estudiaron 1112 ovinos, de los cuales se extrajeron datos en forma individual. Se evaluó estructura poblacional a través de la recolección de información biométrica, morfológica y de composición. Se enviaron al laboratorio 632 muestras de lana que fueron lavadas y clasificadas por denominación y diámetro de fibra. Corriedale, Hampshire Down, Criolla, Merino, Texel, Pampinta y Karacul fueron las denominaciones encontradas. La media de peso de vellón fue de 3.59 kg y el 75.44% de las muestras presento un diámetro de fibra superior a 28&#956;. Las categorías hembra y macho, por explotación reflejó más de la mitad de animales adultos y un bajo porcentaje de animales de reposición. El porcentaje de carneros por explotación fue mayor al aconsejado para la región. Se concluye que los rebaños ovinos existentes en el área evaluada responden en su mayoría al tipo lana grueso y biotipo animal carnicero. En cuanto a la estructura de los rebaños existe una desproporción que tiende a afectar la eficiencia de los parámetros productivos. ","palabras_clave":"PROVINCIA DE LA PAMPA|LANA GRUESA|BIOTIPO CARNICERO|ESTRUCTURA DE LA MAJADA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3751,"idioma_id":4,"titulo":"Detection of Staphylococcus aureus adhesion and biofilm-producing genes and their expression during internalization in bovine mammary epithelial cells","resumen":"Staphylococcus aureus is one of the most prevalent pathogens isolated from bovine mastitis, causing chronic intramammary infections (IMI) that limit profitable dairying. The course of infection is often associated with factors both related to the host and the bacterium. Aims of this study were to select S. aureus isolates from bovine IMI with different genotypic profiles harboring genes involved in adherence and biofilm production, to determine the behavior of these strains in contact with bovine mammary epithelial cells (MAC-T) and the expression of those genes during bacterial-cell early interactions. The genetic diversity of 20 S. aureus strains that were isolated from milk samples taken from cows with persistent-P and non-persistent-NP IMI was high, discriminated into 13 fingerprint groups. The occurrence of genes coding for S. aureus surface proteins (clfA, clfB, fnbA, fnbB, fib, cna) and biofilm formation (icaA, icaD, icaC, bap) and in vitro biofilm-forming ability was not related to strain clinical origin (NP or P). Internalization of S. aureus into MAC-T cells was strain-dependent and internalized bacteria overexpressed adherence and biofilm-forming genes compared with those that remained in the supernatant of co-cultures; particularly those genes encoding FnBPs and IcaD. Strains yielding highest invasion percentages were those able to overexpress fnBP, irrespectively of the presence of other evaluated genes. Strains from NP IMI showed a greater multiplication capacity in vitro compared with strains from P IMI. These results provide new insights about S. aureus differential gene expression of adhesion-internalization factors during early interaction with mammary epithelial cells.","palabras_clave":"Staphylococcus aureus|gene expression|mammary epithelial cells|bovine mastitis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3752,"idioma_id":4,"titulo":"Intrinsic, Extrinsic and Endoplasmic Reticulum stress-induced apoptosis in RK13 cells infected with Equine Arteritis Virus","resumen":"The modulation of the expression of caspases by viruses influences the cell survival of different cell types. Equine arteritis virus (EAV) induces apoptosis of BHK21 and Vero cell lines, but it is not known whether EAV induces apoptosis in RK13 cells, a common cell line routinely used in EAV diagnosis and research. In this study, we determined that caspase-3 expression was triggered after infection of RK13 cells with EAV in a time20and dose-dependent manner. We also detected caspase-8 and caspase-9 activation, indicating the stimulation of both extrinsic and intrinsic apoptosis pathways. Finally, we found caspase-12 activation, an indicator of endoplasmic reticulum stress-inducedapoptosis. The variability observed in the apoptotic response in the different cell lines demonstrates that apoptosis depends on the distinctive sensitivity of each cell line usedfor investigation.","palabras_clave":"equine arteritis virus|apoptosis|RK13 cells"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3753,"idioma_id":5,"titulo":"La localización de los servicios colectivos en entornos urbanos: el caso de la Universidad Nacional de Tres De Febrero","resumen":"En el marco de los estudios que indagan en laproblemática de la localización de los servicios colectivos en contextosurbanos, el presente artículo se propone analizar la distribución espacial delos estudiantes de diversas carreras de la Universidad Nacional de Tres deFebrero, indagando en la localización y características sociohabitacionales desus entornos de residencia y las diferencias que se plantean entre carreras ycohortes diversas. Para ello se propone un abordaje metodológico de tipocuantitativo, comparativo entre dos carreras únicas en el contextometropolitano (las licenciaturas en Estadística y en Sistemas de InformaciónGeográfica) y dos carreras más ?tradicionales? (las licenciaturas en Geografíay en Administración de Empresas), y entre cohortes de ingresantes, retomandopara ello los desarrollos de la geografía cuantitativa y las potencialidadesanalíticas que ofrecen los Sistemas de Información Geográfica para el análisissocioespacial.","palabras_clave":"LOCALIZACIÓN DE SERVICIOS COLECTIVOS|DISTRIBUCIÓN ESPACIAL|EDUCACIÓN UNIVERSITARIA|REGIÓN METROPOLITANA DE BUENOS AIRES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3754,"idioma_id":4,"titulo":"Identification of new palmitoylated proteins in Toxoplasma gondii","resumen":"Protein palmitoylation has been shown to be an important post-translational modification in eukaryotic cells. This modification alters the localization and\/or the function of the targeted protein. In recent years, protein palmitoylation has risen in importance in apicomplexan parasites as well. In Toxoplasma gondii, some proteins have been reported to be modified by palmitate. With the development of new techniques that allow the isolation of palmitoylated proteins, this significant post-translational modification has begun to be studied in more detail in T. gondii. Here we describe the palmitoylome of the tachyzoite stage of T. gondii using a combination of the acyl-biotin exchange chemistry method and mass spectrometry analysis. We identified 401 proteins found in multiple cellular compartments, with a wide range of functions that vary from metabolic processes, gliding and host-cell invasion to even regulation of transcription and translation. Besides, we found that more rhoptry proteins than the ones already described for Toxoplasma are palmitoylated, suggesting an important role for this modification in the invasion mechanism of the host-cell. This study documents that protein palmitoylation is a common modification in T. gondii that could have an impact on different cellular processes.","palabras_clave":"Toxoplasma gondii|acyl-biotin exchange|palmitoylome|protein identification|rhoptry|host-cell invasion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3755,"idioma_id":4,"titulo":"Amoxicillin-loaded electrospun nanocomposite membranes for dental applications","resumen":"Electrospun nanocomposite matrices based on poly(&#949;-caprolactone) (PCL), nanohydroxyapatite (nHAp) and amoxicillin (AMX) were designed and investigated fordental applications. nHAp provides good biocompatibility, bioactivity,osteoconductivity and osteoinductivity properties, and AMX, as antibiotic model,controls and\/or reduces bacterial contamination of periodontal defects while enhancing tissue regeneration. A series of polymeric nanocomposites was obtained by varying both the antibiotic and nHAp contents. Fibrous membranes of different compositions were obtained by electrospinning technique, and morphological, thermal, mechanical and surface properties were characterized. The incorporation of AMX seemed to alter the nHAp distribution within the microfibrous matrix. The interaction between AMX and nHAp affected the mechanical performance and modulated the antibiotic release behavior. AMX release profiles presented a burst release that depended on nHAp content, followed by a slow release stage where the drug content (85-100%) was released in 3 weeks. The antimicrobial activity of the AMX-loaded membranes was tested with four bacterial strains depended on both the drug and nHAp contents. Extensive mineralization in simulated body fluid (SBF) was evidenced by SEM\/EDX analysis after 21 days. The studied electrospun nanocomposite amoxicillin-loaded membranes could be a promising fibrous-based antibiotic carrier system for dental and tissue engineering applications.","palabras_clave":"antibiotic delivery|electrospun scaffold|hydroxyapatite|amoxicillin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3756,"idioma_id":4,"titulo":"A review of silvopastoral systems in native forests of Nothofagus antarctica in southern Patagonia, Argentina","resumen":"Silvopastoral systems in Nothofagus antarctica (n?ire) forest have become an economical, ecological and productive alternative in Patagonia. Southern Patagonia?s experience over the past 12 years with silvopastoral systems is reviewed. The productivity and nutritive value (crude protein contentand dry matter digestibility) of the understorey grassland were dependent on the interaction of environmental (mainly soil water availability and light intensity) and management factors under the trees and in turn determined animal performance. A method developed for carrying capacity estimation at thepaddock level was based on the potential aboveground net primary production, and values ranged from 85 to 2200 kg DM ha-1 year-1. Planned thinning in secondary forest stands provides wood production and also improves the undestorey DM production by increasing incoming radiation. Within a management plan, a stand?s water stress conditions as well as the use of Reineke?s stand density index are proposed to assist in determining thinning intensities. Livestock production is the main annual income of silvopastoral systems where cattle and mixed livestock production (cattle ? sheep) is the main activity. Animal performance at the whole farm scale is presented by comparing traditional extensive grazing management with an adaptive silvopastoral management that included strategic separation in homogeneous areas (grass steppe, forest and riparian meadows), stocking rate adjustment to grassland net primary production and the protection of regeneration from herbivores browsing. Data from litter decomposition, nutrient cycling and carbon storage studies also are presented. Finally, aspects related to the criteria and indicators to assess n?ire forest?s sustainability under silvopastoral use along with biodiversity conservation issues are presented.","palabras_clave":"Livestock grazing|Silviculture|Sustainability|Carbon|Nutrients"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3757,"idioma_id":4,"titulo":"Phylogeny and systematic of the genus Picnoseus (Coleoptera: Meloidae: Meloinae: Pyrotini) inferred from adult morphological characters","resumen":"The Southern South America species of the genus Picnoseus Solier were revised. Ten valid species for the region in the present study were recognized: Picnoseus circunceptus Fairmaire & Germain [new status]; Picnoseus infelix Campos-Soldini [new status]; Picnoseus gajardoi Denier; Picnoseus laevigatus Denier; Picnoseus marginalis Harold 1870 [new status]; Picnoseus nigropictus (Denier); Picnoseus nitidipennis Fairmaire & Germain; Picnoseus rubrofasciatus Denier and two new species from Huasco: Algarrobal &#61630; Chile were described (Picnoseus barrigaii sp. nov. and Picnoseus spockii sp. nov.). Picnoseus nitidipennis Fairmaire & Germain is synonymized with Picnoseus xnathopterus Harlod. The unknown male and female Picnoseus was described for the first time. Complete redescriptions for all known species, a key for their recognition, habitus photographs, and line drawings of genitalia were provided. Distributional maps and data of species habitats were provided. New records for the species of the genus were given. A preliminary cladistics analysis based on 74 morphological characters was performed in order to determine the phylogenetic relationships of these 10 species of Picnoseus.Key Word: Distribution, Redescription, Picnoseus","palabras_clave":"Picnoseus |Description |phylogenetic |Pyrotini"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3758,"idioma_id":4,"titulo":"Involvement of Extracellular Signal-regulated Kinase 5 in Kinin B1 Receptor Up-regulation in Isolated Human Umbilical Vein","resumen":"The up-regulated kinin B1 receptors exert a pivotal role in modulating inflammatory processes. In isolated human umbilical vein (HUV), kinin B1 receptor is up-regulated as a function of in vitro incubation time and pro-inflammatory stimuli. The aim of this study was to evaluate the involvement of extracellular signal-regulated kinase 5 (ERK5), p38 MAPK, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase 1\/2 (ERK1\/2) on kinin B1 receptor up-regulation process in HUV, employing functional and biochemical methods. Real time PCR analysis revealed for the first time that kinin B1 receptor mRNA expression closely parallels the functional sensitization to kinin B1 receptor selective agonist des-Arg10-Kallidine (DAKD) in HUV. Moreover, the selective inhibition of ERK5, p38 MAPK and JNK, but not ERK1\/2 produced a dose-dependent rightward shift of the concentration-response curves (CRC) to DAKD after 5h-incubation and a reduction in kinin B1 receptor mRNA expression. Biochemical analyses showed that ERK5, p38 MAPK and JNK phosphorylation is maximal during the first 2-h post-isolation followed by a significant reduction in the last 3-h. None of the treatments modified the responses to serotonin (5-HT), an unrelated agonist, suggesting a specific effect on kinin B1 receptor up-regulation. The present work provides for the first time pharmacological evidence indicating that ERK5 plays a significant role on kinin B1 receptor up-regulation. Furthermore, we confirm the relevance of p38 MAPK and JNK as well as the lack of effect of ERK1\/2 in this process. This study may contribute for a better understanding of MAPK involvement in inflammatory and immunological diseases.","palabras_clave":"AP-1|ANTI-INFLAMMATORY DRUGS|BRADYKININ RECEPTORS|KINASES|RECEPTOR-UP REGULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3759,"idioma_id":4,"titulo":"Interaction between a mobile and a sessile species in SW Atlantic mudflats: Lack of negative effects of the mud crab Cyrtograpsus angulatus Dana, 1851 on filtration rate and growth of the infaunal clam Tagelus plebeius (Lightfoot, 1786)","resumen":"Biogenic modifications of benthic environment by organism´s activities like feeding, burrowing or excretion have in general negative implications for cohabiting sessile infauna, especially for suspension-feeders. However, the outcome of these interactions depends on several biological and physical characteristics and thus, it is not easily predictable in soft bottom environments. The stout razor clam Tagelus plebeius, an estuarine, infaunal and sessile filter feeder species, coexists in Mar Chiquita coastal lagoon (Argentina, 37° 32&#8242;S, 57° 19&#8242;W) with the mud crab Cyrtograpsus angulatus, a very mobile benthic consumer. Due to the intense crab activity and consequent habitat transformation, we predicted negative effects on clams´ filtration rate and their growth. However, an inclusion experiment showed that T. plebeius filtration rate was higher in enclosures when crabs were present responding to enhanced turbidity and chlorophyll a. Additionally, another experiment showed that mean condition index as well as mean shell growth increment of clams was not negatively affected by crabs, although maximum values of shell growth increments (mm) were smaller in crab inclusions. Our findings suggest that the clams can exploit the increased food availability (i.e., Chl a) generated by resuspension and are also not affected by the disturbance that crabs can make over sediment surface. In conclusion, contrary to the generalized outcome proposed for this kind of interaction, crabs do not have a negative effect on T. plebeius","palabras_clave":"BIOGENIC HABITAT MODIFICATIONS |TAGELUS PLEBEIUS|CYRTOGRAPSUS ANGULATUS|FILTRATION RATE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3760,"idioma_id":4,"titulo":"Photoacoustic Determination of Speed of Sound in Binary Mixtures of Water and Ethyl and Methyl Alcohol","resumen":"The speed of sound in binary aqueous solutions of two alcohols by means of the pulsed photoacoustic technique has been determined. Hereby an analysis of the relationships of excess thermodynamic properties of the mixture to the speed of sound in it is presented. It is also shown that neither the Grüneisen coefficient nor the absorption coefficient of the mixtures are additive functions of the composition. The advantage of this method of speed determination lies in its inherent simplicity and the good precision attained. It can be used in tomography of water in different phantoms and potentially in tissues of biological origin.","palabras_clave":"Speed of sound|Photoacustics|Mixtures"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3761,"idioma_id":4,"titulo":"Supramolecular Assembly of pH-Sensitive Triphenylene Derived &#960;-Gelators and Their Application as Molecular Template for the Preparation of Silica Nanotubes","resumen":"The gelation properties and mode of self-assembly of six asymmetrical hexaether triphenylene derivatives mono-functionalized with carboxylic and primary amine groups were investigated. The presence of a carboxylic and amine group attached to the triphenylene core generated stable, thermo- and pH-sensitive supramolecular &#960;-organogels with a reversible response to both stimuli. In order to understand the gelation process, we studied the effect of the spacer length and found a different gelation scope for the acid and basic derivatives that accounts for a different supramolecular self-assembly. The presence of the basic group on the amino derivatives was used to guide and catalyze the templated in situ sol-gel polymerization of TEOS and allowed us, under controlled hydrolytic conditions, to prepare an entangled fibrillar network of silica nanotubes.","palabras_clave":"triphenylene|pH-sensitive|organogel|supramolecular gel"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3762,"idioma_id":5,"titulo":"Sistemas De Información: Competencias Profesionales 2020","resumen":"Varios autores advierten la obligación de adecuar la preparaciónobtenida en el entorno universitario a las necesidades profesionales que lasociedad demanda y considerar las competencias profesionales a la hora dediseñar nuevas titulaciones. Sin embargo lo cierto es que en la actualidadmuchas de las titulaciones que se imparten en las universidades europeas nocumplen este requisito, manteniendo una distancia importante entre el perfildel egresado y el perfil profesional que demanda la sociedad. Se hanemprendido algunas experiencias, considerando tanto los ámbitosacadémicos como profesionales, a fin de facilitar la integración entre los dosámbitos de interés. El presente estudio tuvo por objetivo definir lascompetencias disciplinares del profesional informático que responda a lasexigencias de los organismos destinatarios de los servicios que presta estaprofesión. Dada la necesidad de especificar las competencias profesionalesdel informático, adaptadas al contexto histórico, se definen las competenciasque emergen de los perfiles profesionales vigentes luego de someterlas a lavaloración de los colectivos que tienen incumbencia real en esta definición.En el trabajo se propuso efectuar una búsqueda de las competenciasprofesionales vigentes en carreras afines de instituciones de reconocidatrayectoria, compilando aquellas más representativas del desempeñoprofesional en esta disciplina. Se analizaron las competencias seleccionadas,a la luz de los objetivos de la carrera, con el propósito de someterlo al juiciode empleadores, docentes, estudiantes y egresados. Una vez valoradas porlos diferentes colectivos, se definieron las competencias profesionales quereunieron los requisitos identificados en el estudio y se extrajeron susconclusiones. El análisis de los resultados se adecuó a la metodologíautilizada por Maldonado Rojas (2007: 235-237), utilizando las frecuenciasobservadas en cada valor de una escala codificada en categoríasinterpretativas. Las competencias que formaron parte de la propuesta finalfueron aquellas que se ubicaron en las categorías interpretativas iguales osuperiores al nivel ?Satisfactorio?. El estudio describe el porcentaje desujetos que adhiere a cada una de las opciones de la escala. Desde el enfoqueinvestigativo adoptado, se estima que el listado elaborado durante el procesoha resultado adecuado al perfil profesional del informático 2020, dado que el97,6% de las competencias propuestas se situaron en los nivelesinterpretativos de relevancia Satisfactorio y Óptimo. A partir de las 80competencias profesionales propuestas, se espera contribuir con los diseñoscurriculares de las terminales de las carreas de informática del sistemaeducativo latinoamericano, o de aquellos correspondientes a las profesionesafines en otros países.","palabras_clave":"Competencias profesionales|Formación por competencias|Perfil profesional|Sistemas de información"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3763,"idioma_id":4,"titulo":"HEMIN INDUCES MITOPHAGY IN A LEUKEMIC ERYTHROBLAST CELL LINE","resumen":"Background InformationIn eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some organelles (such as mitochondria) that are not necessary in the mature red blood cells. Nevertheless, the role of autophagy in erythrocyte maturation has not been fully established.ResultsHere, we have demonstrated that hemin (a physiological erythroid maturation stimulator) is able to induce the expression of critical autophagic genes (i.e., Map1a1b (LC3), Beclin&#8208;1 gen, Atg5) in an erythroleukemia cell type. We have also shown that hemin increased the size of autophagic vacuoles which were labelled with LC3 and the degradative lysosomal marker dye quenched&#8208;bovine serum albumin. In addition, we have determined by Western blot a rise in the lipidated form of the autophagic protein LC3 (i.e., LC3&#8208;II) upon hemin treatment. Moreover, we provide evidence that hemin induces mitochondrial membrane depolarisation and that mitochondria sequestration by autophagy requires the active form of the NIX protein.ConclusionsWe have found that the physiological erythroid maturation stimulator hemin is able to induce mitophagy in K562 cells, and that the autophagy adaptor NIX is necessary for mitophagy progression. K562 cells have been used as a relevant model to determine the possible therapeutic role of new differentiating compounds.SignificanceIt has been proposed that autophagy induction is a feasible new therapeutic key in fighting cancer. Our results suggest that hemin is favoring erythroid maturation by inducing an autophagic response in K562 cells, being a possible therapeutic candidate that may help in the chronic myelogenous leukemia (CML) treatment.","palabras_clave":"AUTOPHAGY|ERYTHROID MATURATION|HEMIN|K562"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3764,"idioma_id":4,"titulo":"Production of Pseudorabies virus recombinant glycoprotein B and its use in an agar gel immunodiffusion (AGID) test for detection of antibodies with sensitivity and specificity equal to the virus neutralization assay","resumen":"Pseudorabies virus (PrV) causes Aujeszky?s disease (AD), which affects mainly swine, but also cattle, sheep, and wild animals, resulting in substantial economic losses due to animal mortality and lost productivity worldwide. To combat PrV, eradication programs using PrV strains lacking the gene encoding glycoprotein E (gE) are ongoing in several countries. These eradication programs have generated a currently unmet demand for affordable, easy-to-use, and sensitive tests that can detect PrV infection in pigs infected with either wild-type virus or vaccine strain (gE-deleted) virus. To meet this demand, we used the baculovirus-insect cell system to produce recombinant glycoprotein B (gB) as antigen for an immune assay. The high GC-content (70% average) of the gB gene from the Argentinian PrV CL15 strain necessitated the use of betaine as a PCR enhancer to amplify the extracellular domain. Recombinant gB was expressed at high levels and reacted strongly with sera from PrV infected pigs. We used the recombinant gB to develop an agar gel immunodiffusion (AGID) test for detection of PrV antibodies.  Compared to the gold standard virus neutralization (VN) assay, the AGID sensitivity and specificity were 95% and 96.6% respectively. Thus, recombinant gB produced in the baculovirus-insect cell system is a viable source of antigen for the detection of PrV antibodies in AGID tests. Considering its relatively lower cost, simplicity of use and result interpretation, our AGID is a valuable alternative tool to the VN assay.","palabras_clave":"Pseudorrbies|AGID|gB"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3765,"idioma_id":4,"titulo":"Risk Analysis of Acute Or Chronic Exposure to Arsenic of the Inhabitants in a District of Buenos Aires, Argentina","resumen":"The arsenic occurrence in the water constitutes a serious world health concern due to its toxicity. Depending on the intensity and duration of exposure, this element can be acutely lethal or may have a wide range of health effects in humans and animals. In Argentina, the origin of arsenic is mainly natural, and related to different geological processes. The Argentinean concern about arsenic and its influence on human health dates back to the previous  century.  The  disease  ascribed  to  arsenic  contamination  was  called  ?chronic regional endemic hydroarsenism?. It is produced by the consumption of water with high levels of this element. In our study, we focused in La Matanza district, a very populated site in the Buenos Aires Province. An increasing concern of the inhabitants of the area regarding  health  problems  was  detected.  In  order  to  establish  a  full  view  of  arsenic exposure in the area, several matrices and targets were analyzed. As matrices, water and soil samples were analyzed. As targets, canine and human hair was studied. The aim of this  study  was  to  investigate  acute  and  chronic  exposure  to  arsenic  of  La  Matanza inhabitants.","palabras_clave":"Arsenic;  |canine hair;| chronic regional endemic hydroarsenism|water contamination;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3766,"idioma_id":4,"titulo":"Diet mixing and condensed tannins help explain foraging preferences by Creole goats facing the physical and chemical diversity of  native woody plants in the central Monte desert (Argentina)","resumen":"The aim of this study was to understand Creole goats´ preference for native forage species in the central Monte desert of Argentina. To achieve this goal, cafeteria-style experiments were conducted with thirty female Creole goats (2 years old; 44 ± 1.4 kg) which were offered five woody forage species goats typically ingest in this region: Two tanniniferous (Tricomaria usillo, Mimosa ephedroides) and three non-tanniniferous shrubs (Prosopis flexuosa, Capparis atamisquea, Atriplex lampa). In Experiment 1, goats were assigned to three groups (n = 10); two groups were offered single tannin-containing shrubs as their basal diet (T. usillo; SDTU or M. ephedroides, SDME), whereas the third group received a combination of all five forages (Mixed diet of forages, MD). After a fifteen-day adaptation period, half the animals in each group (n=5) were dosed with polyethylene glycol (PEG), a polymer that neutralizes the negative effects of tannins, whereas the other half (Control) were not dosed (CG). Daily dry matter intake (DMI) and in vivo apparent digestibility of the diet (ADD) and nitrogen (ADN) was determined. For goats fed MD, preference was estimated based on the DMI of each of the forages offered. Jugular blood samples were collected on the last day of the experiment to determine concentrations of blood urea nitrogen (BUN) and serum metabolites indicative of liver damage. In experiment 2, intake rates (IR) of the five forage species were estimated. No significant differences in DMI were detected among treatments. However, goats offered a choice of forages (MD) in the CG treatment had greater diet digestibility and lower BUN than animals fed the single shrubs, showing evidence of a nutritional benefit with dietary diversity. Goats changed their forage preference in response to PEG supplementation. Animals in the CG treatment preferred M. ephedroides whereas animals in the PEGG treatment preferred A. lampa. There was a positive correlation between forage preference and IR of crude protein (r = 0.65; P < 0.001) in PEGG goats, and between forage preference and IR of total tannins (r = 0.77, P < 0.001) in CG goats. When biological effects of condensed tannins were attenuated by PEG goats switched their preference from forages which offered the greatest IR of total tannins (i.e., M. ephedroides) to those which led to the greatest IR of crude protein (i.e., A. lampa). In summary, a mixed diet led to greater nutritional benefits than single diets which contribute to explain the diverse array of food items goats typically show when browsing in the central Monte desert of Argentina. Our results also show that CP, tannins and plant structure (which offer variable intake rates) play significant roles in goats´ foraging preferences in this environment.","palabras_clave":"NATIVE FORAGE SPECIES|PREFERENCE|PEG SUPLEMENTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3767,"idioma_id":4,"titulo":"Comparison of phase recovery methods in spiral speckle pattern interferometry correlation fringes","resumen":"Spiral interferometry can be used as a solution to the problem of sign ambiguity presented in the conventional speckle pattern interferometric technique when the optical phase needs to be reconstructed from a single closed fringe system. Depressions and elevations of the topography corresponding to the object deformation are distinguished by the direction of rotation of the local spiral fringe pattern. In this work, we implement and compare several methods for optical phase reconstruction by analyzing a single image composed by spiral speckle pattern interferometry correlation fringes. The implemented methods are based on contour line demodulation, center line demodulation, spiral phase quadrature transform and the 2D Riesz transform with multivector structure. Contour line and center line demodulation approaches are exclusively dedicated to images containing a fringe system with spiral structure. The others are based on the 2D Riesz transform, being these well known approaches in conventional interferometry. We examine simulated experiments and analyze some of the emerging drawbacks for solving the phase reconstruction problem by using different mean values of speckle size and background noise levels. We also discuss several numerical procedures that can improve the efficiency and robustness of the presented numerical implementations. The performance of the implemented demodulation methods is evaluated by using a universal image quality index and therefore a quantitative comparison is also presented.","palabras_clave":"Phase retrieval|Speckle interferometry|Spiral interferometry|Optical inspection|Image processing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3768,"idioma_id":4,"titulo":"Oxidative stress-induced premature senescence dysregulates VEGF and CFH expression in retinal pigment epithelial cells: Implications for Age-related Macular Degeneration","resumen":"Oxidative stress has a critical role in the pathogenesis of Age-related Macular Degeneration (AMD), a multifactorial disease that includes age, gene variants of complement regulatory proteins and smoking as the main risk factors. Stress-induced premature cellular senescence (SIPS) is postulated to contribute to this condition. In this study, we hypothesized that oxidative damage, promoted by endogenous or exogenous sources, could elicit a senescence response in RPE cells, which would in turn dysregulate the expression of major players in AMD pathogenic mechanisms. We showed that exposure of a human RPE cell line (ARPE-19) to a cigarette smoke concentrate (CSC), not only enhanced Reactive Oxygen Species (ROS) levels, but also induced 8-Hydroxydeoxyguanosine-immunoreactive (8-OHdG) DNA lesions and phosphorylated-Histone 2AX-immunoreactive (p-H2AX) nuclear foci. CSC-nuclear damage was followed by premature senescence as shown by positive senescence associated-&#946;-galactosidase (SA-&#946;-Gal) staining, and p16(INK4a) and p21(Waf-Cip1) protein upregulation. N-acetylcysteine (NAC) treatment, a ROS scavenger, decreased senescence markers, thus supporting the role of oxidative damage in CSC-induced senescence activation. ARPE-19 senescent cultures were also established by exposure to hydrogen peroxide (H2O2), which is an endogenous stress source produced in the retina under photo-oxidation conditions. Senescent cells upregulated the proinflammatory cytokines IL-6 and IL-8, the main markers of the senescence-associated secretory phenotype (SASP). Most important, we show for the first time that senescent ARPE-19 cells upregulated vascular endothelial growth factor (VEGF) and simultaneously downregulated complement factor H (CFH) expression. Since both phenomena are involved in AMD pathogenesis, our results support the hypothesis that SIPS could be a principal player in the induction and progression of AMD. Moreover, they would also explain the striking association of this disease with cigarette smoking.","palabras_clave":"RETINA|AGE-RELATED MACULAR DEGENERATION|CIGARETTE SMOKE|COMPLEMENT FACTOR H"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3769,"idioma_id":4,"titulo":"Graph classes with and without powers of bounded clique-width","resumen":"Weinitiate the study of graph classes of power-bounded clique-width, that is, graph classes for which there exist integers k and t such that the kth powers of the graphs are of cliquewidth at most t. We give sufficient and necessary conditions for this property. As our main results, we characterize graph classes of power-bounded clique-width within classesdefined by either one forbidden induced subgraph, or by two connected forbidden induced subgraphs. We also show that for every positive integer k, there exists a graph class such that the kth powers of graphs in the class form a class of bounded clique-width, while this is not the case for any smaller power.","palabras_clave":"Clique-width|Power of a graph|Hereditary graph class|Power-bounded clique-width"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3770,"idioma_id":4,"titulo":"Disc Large 1 expression is altered by Human Papillomavirus E6\/E7 proteins in organotypic cultures of human keratinocytes.","resumen":"Loss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human Disc Large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HPV-associated cervical neoplastic lesions, but the mechanisms beyond this remain unknown. In this study, we analyzed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6\/E7 proteins were expressed in organotypic raft cultures. The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. These findings were corroborated in cultures bearing the entire HPV-18 genome. Interestingly, changes in tissue distribution and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6\/E7 proteins; suggesting a conserved function among different HPV types. However, for low-risk HPVs, the subcellular localization of DLG1 at cell-to-cell contacts was predominantly maintained. This report offers new evidence of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions.","palabras_clave":"organotypic raft|HPV E6 and E7|polarity|DLG1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3771,"idioma_id":4,"titulo":"Molecular detection of bovine noroviruses in Argentinean dairy calves:  circulation of a tentative new genotype","resumen":"Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces fromdiarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America.","palabras_clave":"Norovirus|Diarrhea|Bovine|Genotype|Argentina|Recombination"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3772,"idioma_id":4,"titulo":"Distribution of bovine alpha-herpesviruses and expression of toll-like receptors in the respiratory system of experimentally infected calves","resumen":"This study provides an initial analysis of the toll-like receptors (TLRs) that might be implicated in alpha-herpesvirus infection of the bovine respiratory system. A significant variation in the expression of TLR3 and TLRs 7-9 during bovine herpesvirus type 1 (BoHV-1) and 5 (BoHV-5) acute infections and particularly an up-regulation during viral reactivation in respiratory tissues has been demonstrated. Furthermore, viral distribution in the respiratory tract of BoHV-1- and BoHV-5-infected calves at different stages of the infectious cycle was analysed. The wide distribution of BoHV DNA in the respiratory tract during acute infection was restricted during latent infection and the subsequent reactivation of BoHV-1 and BoHV-5. Overall, the findings presented here contribute to the knowledge on the replication and dissemination of bovine alpha-herpesviruses. Furthermore, some of the immune factors triggered in the host that determine the different outcomes of infection by two closely related pathogens of cattle have been elucidated.","palabras_clave":"Toll-like receptors|Expression patterns|Bovine alpha-herpesviruses|Respiratory system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3773,"idioma_id":4,"titulo":"Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes","resumen":"Aged testes undergo profound histological and morphological alterations leading to a reduced functionality. Here, we investigated whether variations in longevity affect the development of local inflammatory processes, the oxidative state and the occurrence of apoptotic events in the testis. To this aim, well-established mouse models with delayed (growth hormone releasing hormone-knockout and Ames dwarf mice) or accelerated (growth hormone-transgenic mice) aging were used. We hereby show that the testes of short-lived mice show a significant increase in cyclooxygenase 2 expression, PGD2 production, lipid peroxidation, antioxidant enzymes expression, local macrophages and TUNEL-positive germ cells numbers, and the levels of both pro-caspase-3 and cleaved caspase-3. In contrast, although the expression of antioxidant enzymes remained unchanged in testes of long-lived mice, the remainder of the parameters assessed showed a significant reduction. This study provides novel evidence that longevity confers anti-inflammatory, anti-oxidant and anti-apoptotic capacities to the adult testis. Oppositely, short-lived mice suffer testicular inflammatory, oxidative and apoptotic processes.","palabras_clave":"AGING|APOPTOSIS|OXIDATIVE STRESS|TESTIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3774,"idioma_id":4,"titulo":"Sodium Caseinate\/Sunflower Oil Emulsion-Based Gels for Structuring Food","resumen":"Protein gels have attired attention since they allow structuring foods with no trans or saturated fats. The effects of protein concentration and sucrose addition on gelation kinetics and on physical properties of sodium caseinate (NaCas)\/sunflower oil emulsion-based gels were studied by two methods: a new application of backscattering of light (BS) using a Turbiscan equipment and by dynamic oscillatory rheology. Structure of gels was also described by confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS). T gel values decreased with increasing sucrose or NaCas concentration. BS method sensed early changes in structure, while rheological measurements were less sensitive to those changes. However, tendencies found by rheological measurements were the same as the ones found by BS experiments. CLSM images of gels formed from emulsions containing high sucrose and protein concentrations had big oil droplets that were not present in initial emulsions. Gels with sucrose concentrations between 15 and 30 wt\/wt% released oil. SAXS patterns showed that NaCas nanoaggregate sizes in the aqueous phase were smaller with increasing sucrose concentration. Polar groups of protein interacted with sucrose, and therefore, interactions among protein molecules diminished. As a result of weaker protein molecule interactions, nanoaggregates were smaller. However, this effect was beneficial. In the macroscale, rheological properties and visual appearance of gels were improved. The gel formulated with 5 wt\/wt% NaCas and 10 wt\/wt% sucrose had a smooth surface and was stable to syneresis and oil release. This formulation was a good alternative to trans fat.","palabras_clave":"CASEINATE GELS|TURBISCAN|RHEOLOGY|SAXS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3775,"idioma_id":5,"titulo":"Formación en  investigación  educativa en la sociedad digital. Una experiencia innovadora de enseñanza en el nivel superior en el contexto latinoamericano.","resumen":"Desde la Cátedra Investigación Educativa II de la Universidad Nacional de San Luis,Argentina, nos preguntamos acerca de la pertinencia de los contenidos y valor de lasestrategias de enseñanza que se utilizan para formar en investigación a los futurosprofesionales de la educación y asumimos el reto de repensar la disciplina misma y suenseñanza en el contexto de la sociedad actual.Se analizó la investigación a la luz de las nueva tecnologías y el desarrollo de lo digital, seindagó la expresión que esto adopta en el contexto latinoamericano y se planificó unaexperiencia de trabajo innovadora para los estudiantes de cuarto año del Profesorado y laLicenciatura en Ciencias de la Educación, con un enfoque de enseñanza centrado en elestudiante, y acciones que devienen de los principios del aprendizaje invertido, aprendizajeextendido y trabajo colaborativo.El artículo profundiza la relación que se establece entre investigación educativa, tecnologíay enseñanza de la investigación; y promueve la reflexión en torno al impacto que la mismapuede tener sobre los contenidos de la formación de investigadores, los procesos deformación, el perfil de los investigadores y la investigación educativa misma. Relata losprincipios de los que se partió en la experiencia, el modo como se trabajó, los aspectos másvalorados y los considerados críticos o que requieren más atención y estudio. Cierra conunas reflexiones generales.","palabras_clave":"Investigacion educativ a|innovación|Enseñanza de la Investigación educativa|Educacion superior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3776,"idioma_id":4,"titulo":"Outcomes of the Clean Development Mechanism in Argentina","resumen":"This paper summarizes the results of the implementation of the CDM in Argentina during the first period of commitment. From a total of sixty five projects ideas, forty four achieved registration while the rest remained approved at national level (12), under evaluation (2), suspended (5) or rejected (2). Most of the projects focused on the generation of electricity from renewable energy, mainly wind energy and the use of landfill gas and methane for energy. Emissions reductions achieved 33.4% of the expected CERs up to 2012 with strong differences between sectors. Results demonstrated that under the current Argentine´s energy policy framework, the income by the selling of CERs covered less than 6% of the incremental costs for renewable energy projects. A sensitivity analysis to evaluate the impact of CDM in the coverage of incremental costs for renewable energy based on the prices of both the energy in the local market and the CER demonstrated that the best conditions would only cover 15% of those costs. The contribution of CDM to technology transfer in Argentina was little considering that 45% qualified as type III where technological learning and capacity building were only limited at the level of operation and maintenance of a foreign technology. Domestic and external barriers that prevented a better performance of CDM projects in terms of mitigation, technology transfer and the contribution to a sustainable development were also analyzed.","palabras_clave":"CLEAN DEVELOPMENT MECHANISM|GHG EMISSIONS|INCREMENTAL COST|RENEWABLE ENERGY ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3777,"idioma_id":4,"titulo":"AJMU: An Aspect-Oriented Framework for Evaluating the Usability of WIMP Applications","resumen":"used for automatic evaluation of the usability of WIMP applications, with the aim of monitoring events and GUI components and recording data in logs. A weakness of AOP approaches is low semantic value and a low level of abstraction of the results. One possible solution to overcome these limitations is to use an AO framework for assessing the usability of user tasks. However, little research has been devoted the development of AO frameworks from scratch. Although different design and programming patterns are available, insufficient experience has been reported regarding their application in the development of frameworks. This paper presents AJMU, an AO framework for the automatic evaluation of the usability of user tasks in desktop applications. AJMU was developed from scratch, using AO patterns. This paper also reports on experiments involving AJMU?s instantiation with real applications.","palabras_clave":"USABILITY|FRAMEWORK|ASPECT-ORIENTED PROGRAMMING|DESIGN PATTERNS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3778,"idioma_id":5,"titulo":"Flexible Pseudorandom Number Generator for  Tinnitus Treatment Implemented on a DSPic","resumen":"Treatment  of  tinnitus  with  masking  sounds  has reach a significant developed in recent years. It is mainly because it  has  been  possible  to  implement  noise  sinthesizers  based  on random number  generators  in  digital  signal  processors (DSP), which form a part of almost any digital hearing aid device.  One limitation  of  these  methods  is  that  limitations  of  the  DSP architecture  prevent  pseudo  white  noise  of  being  generated conform  to  a  real  white  noise  statistics. In  this  paper,  a technique to generate adittive white gaussian noise (AWGN) or noise  with  uniform  distribution  using  coefficients  stored  in memory of the DSP program is proposed. An implementation of the  technique  is  carried  out  on  a  dsPIC  from  Microchip  and subjective  experiments  and  experimental  measurements  are performed  to  validate  the  performance  of  the  developed technique. ","palabras_clave":"TRATAMIENTO DE ACÚFENOS|DSP|SISTEMAS EMBEBIDOS|DISPOSITIVO DE ASISTENCIA AUDITIVA|GENERADOR DE NÚMEROS PSEUDOALEATORIOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3779,"idioma_id":4,"titulo":"The Einstein Nanocrystal","resumen":"We study the simplest possible model of nanocrystalconsisting in a simple cubic lattice with a small number of atoms (NA&nbsp;&nbsp;&nbsp;&#820;10 -103), where each atom is linked to its nearest neighbor by aquantum harmonic potential. Some properties (entropy, temperature, specificheat) of the nanocrystal are calculated numerically but exactly within theframework of the microcanonical ensemble. We find that the presence of asurface in the nanocrystal modifies the thermostatistic properties to a greaterextent than the small number of atoms in the system. The specific heat Cv&nbsp; behavessimilarly to the Einstein solid, with an asymptotic value for high temperaturesthat differs from that of the Dulong-Petit law by a term of the order of NA-1\/3 and thatcan be explained easily in terms of the surface. The entropy is non-additive, butthis is due to the presence of the surface and we show that the additivity isrecovered in the thermodynamic limit. Finally, we find that, when calculationsfollow the canonical ensemble, results differ little for small systems (NA = 27) and areinexistent for larger systems (NA= 1000)","palabras_clave":"FEW PARTICLE SYSTEMS |MICROCANONICAL ENSEMBLE|NANOCRYSTAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3780,"idioma_id":4,"titulo":"An update of biocatalytic selective acylation and deacylation of monosaccharides","resumen":"Partially acylatedmonosaccharides (PAMs) exhibit diverse and interesting applications but due totheir polyhydroxylated nature, their synthesis requires regio- andstereoselective reactions. These features are provided by biocatalyticprocesses and in particular by hydrolases, which offer mild conditions andselective routes for the preparation of PAMs. Since this strategy has been extensivelyexplored, the aim of the present review is to update research on enzymaticselective acylation and deacylation of monosaccharides, focusing on enzymaticpreparation of synthetic useful PAMs and drug-monosaccharide conjugatesinvolving PAMs.&nbsp; &nbsp;&nbsp;","palabras_clave":"ACILACION|DESACILACION|BIOCATALISIS|SELECTIVIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3781,"idioma_id":4,"titulo":"Genetic variability and recombination analysis of the coat protein gene of Strawberry mild yellow edge virus","resumen":"Strawberry mild yellow edge virus (SMYEV) hasbeen detected in most of the strawberry production regionsworldwide. However, little is known about differencesbetween distinct isolates. The aim of this study was toenhance the knowledge about the genetic variability ofdifferent SMYEVisolates, exploring the phylogenetic relationshipsand assessing recombinant events among them. The coatprotein (CP) gene of 12 Argentinian SMYEV isolates wassequenced. There were 729 nucleotides (nt) in all of the isolates,encoding a protein of 242 amino acids (aa). Argentinaisolates shared 81.5?99.6 % nucleotide identity. The comparisonof these isolates with 30 SMYEV sequences from othercountries published in the GenBank, revealed an identity rangingfrom 81.6 to 99 %. The phylogenetic analysis showed thepresence of four possible subgroups, with the Argentinian isolatesbeing included in all of them. Recombination analysisindicated that 16?2 (KP 284155) Argentinian and AJ577342Chilean isolates are recombinant and that they are a result ofrecombination events where parts of the genome were exchangedbetween different SMYEV sequences.","palabras_clave":"Strawberry|Virus|SMYEV|Virus taxonomy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3782,"idioma_id":4,"titulo":"Alternative methodology for traditional interventions: a colonial painting and its lining with the nap bond method","resumen":"Lining treatments used in the conservation-restoration field can be classified according to the adhesive used. Traditional methods include those based on glue-starch and wax-resin whilst those using synthetic adhesives are considered alternative methods. Rise of new materials and mechanical equipment like the low-pressure table expanded possibilities of intervention. However, alternative methods require previous exhaustive testing of procedures, tools and supplies. This paper describes research carried out by an interdisciplinary team of conservators, historians and chemists from the IIPC-TAREA that elucidated fundamental aspects of a painting of San Luis Gonzaga from the South American colonial period, deteriorated but with an important documentary value. In the case here presented a minimal intervention restoration criterion was applied and the nap bond method used proved to be the most suitable alternative lining for this particular situation.","palabras_clave":"NAP BOND|LINING|MINIMAL INTERVENTION|REVERSIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3783,"idioma_id":5,"titulo":"Herramienta de apoyo para las clases impartidas en laboratorios de informática: construcción de una solución en red basada en IPv6","resumen":"A partir de la experiencia docente universitaria, fue posible evidenciar algunos problemas que surgen, al utilizar los laboratorios de informática, en los procesos de enseñanza-aprendizaje. De hecho detectar una necesidad es el primer paso para planificar una solución. En este caso dicha solución ha sido el desarrollo de una aplicación en red que permita servir de soporte a las clases impartidas en el ámbito de los laboratorios de informática. Tomando en cuenta los múltiples usos de los mismos y la diversidad de contenidos de las materias, la solución busca integrar distintos recursos que agilizan algunas tareas requeridas por parte de los docentes y alumnos en la clase, para lo cual se desarrolló una aplicación en red basada en IPv6 que permite diferentes modos comunicacionales, admite el envío de archivos e imágenes, visualizar videos en simultaneo y, también cuenta con la posibilidad de chat.","palabras_clave":"REDES DE COMUNICACIÓN|IPV6|ENSEÑANZA SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3784,"idioma_id":4,"titulo":"Different canopy openings affect underground traits in herbaceous plants of a southern forest in Patagonia","resumen":"AimsForest canopy openings modify the natural environment, producing changes in light quality and intensity, precipitation and temperature. In turn, these changes promote the acclimation of understory species. However, little work has been done on underground responses to those environmental changes. The objective of this work was to determine how Osmorhiza depauperata, Phleum alpinum and Poa pratensis change its root length density and root colonization by mycorrhiza as a function of light availability in a Nothofagus pumilio (i.e., lenga) forest harvested following the variable retention prescription.MethodsWe selected three microenvironments in an old growth forest harvested by the variable retention prescription: aggregated retention, dispersed retention with influence of aggregated retention and dispersed retention. A non-harvested primary forest, similar to the harvested one, was used as a control. Every two months, from October 2008 to April 2009, we took soil cores from randomly selected plants. From these soil cores, root length density and colonization percentage by arbuscular mycorrhizae were estimated.Important FindingsLight availability changed significantly among the microenvironments. In general, root length density was significantly greater in P. pratensis than in P. alpinum and both species greater than in O. depauperata. Light availability increased root length density in all species, although the magnitude of these increases differ among species. Root length density was 187% greater in P. pratensis, 101% in P. alpinum and 94% in O. depauperata in the disperse retention system than in the primary forest. Mycorrhiza colonization percentage was higher in O. depauperata than in P. alpinum and P. pratensis. Also, it was lower in the primary forest than in the harvested microenvironments. Colonization percentages were very low.","palabras_clave":"PATAGONIA|MICORRIZAS|RAICES|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3785,"idioma_id":4,"titulo":"PIGMENTS AND PHOTOSYNTHESIS OF UNDERSTORY GRASSES:  LIGHT IRRADIANCE AND SOIL MOISTURE EFFECTS1","resumen":"Phleum alpinum and Poa pratensis are major forage species that often grow in various environments in Tierra del Fuego, Argentina. To investigate how these species acclimate to the different irradiance of the microenvironments where they grow we performed a greenhouse experiment. Both grass species were exposed to three levels of incident irradiance (I4: 4%; I26: 26%, or I64: 64% of ambient sunlight) and two levels of soil moisture content (M30: 30-50% or M60:60-80% of field capacity) under greenhouse conditions. As irradiance levels increased, the contents of chlorophyll per unit surface area and fresh weight basis increased, and the relationships chlorophyll a\/b and carotenoids\/chlorophyll also increased. Maximum photosynthetic rate and light compensation point increased as light availability also increased. Values for these variables varied with time. However, the relationship of these values between the irradiance treatments was not modified in P. alpinum. Contrarily, temporal changes of those variables determined that the maximum photosynthetic rate was similar to that in March in all treatments in P. pratensis. Results indicated that P. alpinum and P. pratensis were able to acclimate to the various study environments.","palabras_clave":"PHOTOSYNTHESIS|IRRADIANCE|SOIL WATER CONTENT|GRASSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3786,"idioma_id":4,"titulo":"Excess iodide downregulates Na+\/I- symporter gene transcription through activation of PI3K\/Akt pathway","resumen":"Transcriptional mechanisms associated with iodide-induced downregulation of NIS expression remain uncertain. Here, we further analyzed the transcriptional regulation of NIS gene expression by excess iodide using PCCl3 cells. NIS promoter activity was reduced in cells treated for 12e24 h with 105 to 103 M NaI. Site-directed mutagenesis of Pax8 and NF-kB cis-acting elements abrogated the iodide induced NIS transcription repression. Indeed, excess iodide (103 M) excluded Pax8 from the nucleus, decreased p65 total expression and reduced their transcriptional activity. Importantly, p65-Pax8 physical interaction and binding to NIS upstream enhancer were reduced upon iodide treatment. PI3K\/Akt pathway activation by iodide-induced ROS production is involved in the transcriptional repression of NISexpression. In conclusion, the results indicated that excess iodide transcriptionally represses NIS gene expression through the impairment of Pax8 and p65 transcriptional activity. Furthermore, the data presented herein described novel roles for PI3K\/Akt signaling pathway and oxidative status in the thyroid autoregulatory phenomenon.","palabras_clave":"EXCESS IODIDE|SODIUM IODIDE SYMPORTER|PAIRED BOX 8 (PAX8)|PI3K\/AKT PATHWAY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3787,"idioma_id":4,"titulo":"In vitro degradation of electrospun poly(L-lactic acid)\/segmented poly(ester urethane) blends","resumen":"Scaffold degradation behavior is one of the main aspects to be considered in tissue engineering applications. The hydrolytic in vitro degradation of compliant biomimetic electrospun bilayered vascular grafts made of poly(L-lactic acid) and segmented poly(ester urethane) blends was studied. Two polymeric blends were selected based on the natural arteries collagen and elastin composition, structure and functionality. The variation of molecular weight, and thermal and surface properties of the blends and their plain polymers, were analyzed and related to the structural and morphological changes taking place during degradation. The low initial crystallinity and high hydrophilicity of the blends, along with a synergetic outcome produced by blending, led to a higher molecular weight loss. Even more, it was observed that a bilayered graft made from these blends would degrade evenly as a whole. Moreover, the degradation time was found to match with the required for the regeneration processes. These facts make them encouraging for a future application as degradable small-diameter vascular constructs.","palabras_clave":"bioresorbable polymers|electrospinning|hydrolytic degradation|vascular grafts"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3788,"idioma_id":4,"titulo":"Tribological behaviour of nitrided and nitrocarburized carbon steel used to produce engine parts","resumen":"Purpose ? The purpose of this study is to select a proper surface treatment to enhance wear resistance of engine camshafts. The camshaft is arelevant part of a diesel engine which works under torsion, fatigue and wear efforts. They are usually manufactured by casting, forging or machiningfrom forged bar of low alloy steels, and in most cases, the machined surfaces are quenched and tempered by induction heating. After that, in manycases, to withstand the efforts imposed on the active surfaces and improve tribology and fatigue properties, the industry used for decades,thermochemical technologies such as salt bath or gaseous nitriding and nitrocarburizing processes.Design\/methodology\/approach ? This paper studied the effects of plasma nitriding and plasma nitrocarburizing, on the tribological behaviour ofthe steel SAE 1045HM3 proposed to produce camshafts. After the plasma treatments, the change in surface roughness was measured; the modifiedlayers were studied by X-ray techniques and its thickness by optical microscopy. The diffusion zone was evaluated by Vickers microhardnessdeterminations. Tribology tests were performed by pin-on-disc configuration using WC ball as a counterpart.Findings ? Results show that plasma nitrided samples present the best tribological behaviour compared with the nitrocarburized ones; also, theinfluence of the roughness produced by the thermochemical processes appears to be important.Practical implications ? Although both the plasma treatments have been applied for many years, and also reported separately in the scientificliterature, there was no information comparing these two treatments for carbon steels, and also, there is not much about tribology in lubricatedconditions of nitrided and nitrocarburized carbon steels. In fact, it is not proved that the porosity of the nitrocarburized layer is beneficial for wearresistance in lubricated conditions. In this paper, it was proved that at least in the tested conditions, it is not.Originality\/value ? Gas or plasma nitrocarburizing is usually recommended for this kind of applications, although the modified layer is porous. Thispaper attempts to prove that nitriding could be better than nitrocarburizing, even with a thinner white layer.","palabras_clave":"TRIBOLOGY|WEAR|SURFACE ENGINEERING|PLASMA ASSISTED DIFFUSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3789,"idioma_id":4,"titulo":"The adoption of no-till instead of reduced tillage does not improve some soil quality parameters in Argentinean Pampas","resumen":"No-till (NT) has been recognized worldwide as a more suitable system than tillage for enhancing soilquality. However, several concerns remain about its conservative nature, especially when it is performedeither without cover crops or appropriate rotation schedules, and when it is accompanied by the usage ofhigh amounts of agrochemicals. In this paper, we study some soil quality parameters when NT is adoptedinstead of reduced tillage, as well as the relevance of soil physical and chemical properties to explain theimpact of management systems on soil macrofauna. We compared NT and reduced tillage (RT) systems,using natural grasslands (GR) as reference. We hypothesised that (1) soil quality will decline in bothagricultural systems compared to the grassland but this declination will be less in no-till than in reducedtillage, and that (2) the changes in macrofauna community could be explained by changes in physical andchemical soil properties. Soil cover, organic matter, pH, moisture content, bulk density and mechanicalresistance were assessed as indicators of soil physical and chemical quality. Soil macrofauna abundanceand composition was determined by the TSBF method. We rejected ourfirst hypotheses since from theassessed parameters only soil moisture content and spider abundance were favoured in NT compared toRT. Changes caused by both systems in the macrofauna composition (especially in soil inhabitants) weremainly explained by soil physical and chemical attributes. The ordination of sites according to canonicalcorrespondence analyses clearly shows the influence of the management systems in the relationshipbetween macrofauna assemblages and soil physical and chemical parameters; especially in the upper30 cm of soil. GR had both a better soil physical and chemical quality and a higher abundance of the mainmacrofauna taxa (earthworms, beetles and ants) compared to agricultural systems. NT and RT weresimilar, sharing low earthworm and ant abundance and high potworm abundance. Our results show thatadopting NT instead of RT does not favour assessed soil quality parameters. Thus, NT is questioned as asystem which enhances soil quality, at least in the way it is performed by most farmers from ArgentinePampa.","palabras_clave":"AGROECOSYSTEMS |GRASSLAND|SOIL QUALITY |SOIL MACROFAUNA|SOIL COMPACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3790,"idioma_id":4,"titulo":"Effect of Good Agricultural Practices under no-till on litter and soil invertebrates in areas with different soil types","resumen":"Good Agricultural Practices (GAPs) under no-till (NT) includes a mixed crop rotation; cover crops;integrated pest, weed and disease management; nutrient restoration; and a rational use ofagrochemicals. When applied all together, GAPs promotes high productivity, while maintaining theproduction capacity of resources. In the Pampas region of Argentina, there is a need to assess the effects ofthese practices on soils, particularly on soil fauna, as they play an important role in soil functioning. Theaim of this study was to evaluate the effect of the application of GAPs under NT on invertebrates and toassess whether this effect is different between soil types. We hypothesized (1) that GAP will produce anincrease in the abundance, as well as changes in the faunal composition of litter and soil invertebrates; (2)that the effects will differ with soil type, and (3) that the changes in soil invertebrate fauna will beexplained by soil properties. We compared two contrasting NT treatments -with and without GAPapplication-, replicated in three agricultural areas, on different soil types (Entic Haplustolls to TypicArgiudolls) situated across a west?east transect in the Pampas region of Argentina. A positive (Naturalenvironment) and a negative (Conventional tillage) reference sites were included in the comparison.Litter and soil invertebrates and soil properties were assessed at each sampling site. Overall, our resultsindicated that the application of GAPs in productive NTfields increases litter and soil invertebrateabundance and modifies faunal composition. In the litter layer, four of thefive taxa present were favouredby GAPs with an increase in the abundances of ants, prostigmatid mites, earthworms and collembolans.GAPs also induced changes in invertebrate faunal composition, from the initial NO-GAP situation to thepresent state under GAP system. The observed changes in litter and soil invertebrates, changes in faunalabundance and composition can be expected to translate to changes in soil functioning. Our lasthypothesis was partially confirmed in that soil properties have to be considered in the examination ofdifferences in fauna between treatments with there are only subtle differences in practices, as in thepresent study.","palabras_clave":"MESOFAUNA |MACROFAUNA |MONOCULTURE |CONVENTIONAL TILLAGE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3791,"idioma_id":5,"titulo":"INFLUENCIA DE LA COMPOSICION DE LA LECHE EN POLVO SOBRE LA ACTIVIDAD DE AGUA APLICANDO EL MODELO DE GAB","resumen":"El trabajo analiza la influencia sobre la actividad de agua (aw) de las diferentes  composiciones de leche en polvo entera, descremada y parcialmente deslactosada, mediante el estudio de las isotermas de sorción a 25ºC.En forma experimental el trabajo nos permite establecer la relación entre el contenido de humedad de las diversas leches en polvo estudiadas, con su actividad de agua (aw). Los resultados experimentales son evaluados con el modelo referencial de GAB (Guggenheim-Anderson-de Boer)   observando que el trazado de las isotermas y los parámetros del modelo se ajustan satisfactoriamente a los valores experimentales.El trabajo permite observar la clara influencia de la presencia de los monosacáridos (glucosa y galactosa) en la leche parcialmente deslactosada, que reducen la actividad de agua a partir de aw mayores a 0,4.  El contenido graso en la leche entera, al no ser adsorbente se manifiesta en valores mayores para la actividad de agua.","palabras_clave":"ISOTERMAS|DESLACTOSADA|ACTIVIDAD|HUMEDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3792,"idioma_id":4,"titulo":"Evaluation of an autochthonous starter culture on the production of a traditional dry fermented sausage from Chaco (Argentina) at a small-scale facility","resumen":"The performance of a mixed starter culture, SAS-1, comprised of the autochthonous strains Lactobacillus sakei ACU-2 and Staphylococcus vitulinus ACU-10, was evaluated into the production process of a traditional dry sausage. Microbiological, physicochemical and sensory analyses were carried out to accomplish this goal. Results showed an improvement in performance through the introduction of SAS-1; adding mixed starter culture rapidly decreased pH, inhibited the growth of contaminant microorganisms and enhanced the beneficial ones, diminished TBARS, and highlighted color and aroma attributes. However, most influential organoleptic descriptors among consumer acceptance were not affected by the addition of the starter. This starter culture would represent a valuable tool to improve the homogeneity of artisanal manufacture of this traditional food.","palabras_clave":"INDIGENOUS STRAINS|STARTER CULTURE|TRADITIONAL MEAT PRODUCT|DRY SOUSAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3793,"idioma_id":4,"titulo":"Current drug therapy and pharmaceutical challenges for Chagas disease","resumen":"h: 0px; '&gt;One of the most significant health problems in the American continent in terms of human health, andsocioeconomic impact is Chagas disease, caused by the protozoan parasite Trypanosoma cruzi. Infectionwas originally transmitted by reduviid insects, congenitally from mother to fetus, and by oral ingestion insylvatic\/rural environments, but blood transfusions, organ transplants, laboratory accidents, and sharingof contaminated syringes also contribute to modern day transmission. Likewise, Chagas disease used tobe endemic from Northern Mexico to Argentina, but migrations have earned it global. The parasite hasa complex life cycle, infecting different species, and invading a variety of cells ? including muscle andnerve cells of the heart and gastrointestinal tract ? in the mammalian host. Human infection outcomeis a potentially fatal cardiomyopathy, and gastrointestinal tract lesions. In absence of a vaccine, vectorcontrol and treatment of patients are the only tools to control the disease. Unfortunately, the only drugsnow available for Chagas? disease, Nifurtimox and Benznidazole, are relatively toxic for adult patients,and require prolonged administration. Benznidazole is the first choice for Chagas disease treatment dueto its lower side effects than Nifurtimox. However, different strategies are being sought to overcomeBenznidazole?s toxicity including shorter or intermittent administration schedules?either alone or incombination with other drugs. In addition, a long list of compounds has shown trypanocidal activity,ranging from natural products to specially designed molecules, re-purposing drugs commercialized totreat other maladies, and homeopathy. In the present review, we will briefly summarize the upturnsof current treatment of Chagas disease, discuss the increment on research and scientific publicationsabout this topic, and give an overview of the state-of-the-art research aiming to produce an alternativemedication to treat T. cruzi infection.","palabras_clave":"CHAGAS DISEASE|TRYPANOSOMA CRUZI|TRYPANOCIDAL TREATMENT|BENZNIDAZOL|NIFURTIMOX|NEW MEDICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3794,"idioma_id":4,"titulo":"AMPK and PKA interaction in the regulation of survival of liver cancer cells subjected to glucose starvation","resumen":"The signaling pathways that govern survival response in hepatocellular carcinoma (HCC) cells subjected to nutritional restriction have still not been clarified.In this study we showed that HCC cells undergoing glucose deprivation both arrested in G0\/G1 and died mainly by apoptosis. Treatment with the AMPK activator AICAR phenocopied the effect of glucose deprivation on cell survival, whereas AMPK silencing blocked the arrest in cell cycle and the increase in apoptotic death induced by glucose starvation. Both AMPK and PKA were promptly activated after glucose withdrawal. PKA signaling showed a dual role: early during glucose starvation decreased viability and increased it later. We detected greater AMPK phosphorylation (AMPKa1(Ser173)) by PKA in glucose starved than in fed cells associated with diminution of AMPK activation. To better explore this inhibitory effect in our setting, we constructed a cell line stable expressing an AMPK mutant lacking that PKA phosphorylation site: AMPKa1(S173C). Expression of this mutant significantly decreased viability in cells undergoing glucose starvation. Furthermore, the fraction of apoptotic cells after 36 h of glucose deprivation in mutant cells doubled the apoptotic index observed in control cells during the same condition. Two main remarks arise: 1. AMPK is the central signaling kinase in the scenario of cell cycle arrest and death induced by glucose starvation in HCC cells; 2. PKA phosphorylation of Ser173 comes out as a strong control point that limits antitumor effects of AMPK in this situation.","palabras_clave":"AMPK|APOPTOSIS|SIGNALING|PKA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3795,"idioma_id":4,"titulo":"Treatment of endosulfan contaminated water with in vitro plant cell cultures","resumen":"Endosulfan is a POP insecticide still used in many countries. It is commercially available as mixtures of two diastereomers, &#945;- and &#946;-endosulfan, known as Technical Grade Endosulfan (TGE). A laboratory model based on the use of axenic plant cell cultures to study the removal and metabolization of both isomers from contaminated water matrixes was established. Non differences were recorded in the removal of the two individual isomers with the two tested endemic plants, Grindelia pulchella and Tessaria absinthioides. Undifferentiated cultures of both plant species were very efficient to lower endosulfan concentration in spiked solutions. Metabolic fate of TGE was evaluated by analyzing the time course of endosulfan metabolites accumulation in both plant biomass and bio-remediation media. While in G. pulchella we only detected endosulfan sulfate, in T. absinthioides the non-toxic endosulfan alcohol was the main metabolite at 48 h, giving the possibility of designing phytoremediation approaches.","palabras_clave":"PHYTOREMEDIATION|PHYTOMETABOLIZATION|ENDOSULFAN|TESSARIA ABSINTHIOIDES|GRINDELIA PULCHELLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3796,"idioma_id":4,"titulo":"Biotechnological tools to improve bioremediation of phenol by Acinetobacter sp. RTE 1.4.","resumen":"The use of native bacteria is a useful strategy to decontaminate industrial effluents as well as the environment. Acinetobacter sp. RTE1.4, a bacterial strain isolated from polluted environments, constitutes a promising alternative for this purpose since it is able to remove phenol from synthetic solutions and industrial effluents. In this work, this strain was identified at species-level as A. tandoii RTE1.4. Phenol degradation pathway was studied and some reaction intermediates were detected, confirming that this strain degraded phenol through ortho-cleavage of the aromatic ring. Phenol removal assays were carried out in a stirred tank bioreactor and a complete degradation of the contaminant was achieved after only 7 h, at an aeration rate of 3 vvm and at agitation of 600 rpm. Moreover, this bacterium was immobilized into calcium alginate beads and an increase in phenol biodegradation respect to free cells was observed. The immobilized cells were reused for four consecutive cycles and stored at 4 °C for 9 months, during which phenol removal efficiency was maintained. Post removal solutions were evaluated by Microtox® test, showing a toxicity reduction after bacterial treatment. These findings demonstrated that A. tandoii RTE1.4 might be considered as an useful biotechnological tool for an efficient treatment of different solutions contaminated with phenol in bioreactors, either using free or immobilized cells.","palabras_clave":"phenol|bacteria|bioremediation|bioreactor|immobilization|toxicity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3797,"idioma_id":4,"titulo":"Trends and variability in extended ocean color time series in the main reproductive area of the Argentine hake, Merluccius hubbsi (Southwestern Atlantic Ocean)","resumen":"The Argentine hake Merluccius hubbsi is one of the main commercial resources of the Southwest Atlantic region,with a reported catch of 259,202 tons in 2014. Hake recruitment shows high interannual variability, yet theenvironmental and biological factors that influence reproduction are not fully understood. The increasing availabilityof ocean color data presents an opportunity to investigate a wide variety of fundamental topics includingocean primary productivity, climate change, and fisheries, among others. However, differences in the timing,length, and radiometer characteristics of the different missions result in a number of relatively short data recordsthat are not suitable, individually, for the analysis of interdecadal changes. The combination of these datasets toproduce longer time series of consistent data is essential for interpreting variability and trends in key parameters. We analyzed almost 5 years of high spatial resolution overlapping data from the SeaWiFS and MODIS Aqua sensors in the Southwestern Atlantic Ocean to assess differences in chlorophyll concentration retrievals, estimate uncertainties, and develop corrections. Data from SeaWiFS (1997?2006) and corrected MODIS (2007?2015) were analyzed jointly as a N17-year time series of consistent and continuous chlorophyll concentration data, the longest record to date in the region. Trend analyses performed in the main spawning and nursery areas of M. hubbsi revealed significant increases in chlorophyll concentrations since 1997. The environmental factors likely influencing the observed changes and the potential implications for recruitment of M. hubbsi are discussed.","palabras_clave":"Ocean color|SeaWiFS|MODIS|time series|Merluccius hubbsi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3798,"idioma_id":4,"titulo":"Adaptive responses of quinoa  to diverse agroecological environments along an altitudinal gradient in North West Argentina","resumen":"Quinoa is an important Andean grain crop grown in a wide range of tropical and temperate environments.Time to flowering is an important trait determining grain yield. This work aimed to understand howresponses to photoperiod and temperature might alter plant leaf and floral development. To assess thelikely degree of G × E interactions, eleven quinoa accessions from a wide range of environments of originwithin Northwest Argentina were grown in several sowing dates over two seasons at a high altitude site.In a third season at a low altitude site, a subset of six accessions planted in pots in the field was exposedto two artificially extended and a control (natural) photoperiod. Time to the appearance of floral budsand anthesis were recorded as was leaf number. A photothermal model developed for quinoa was usedto compare responses to photoperiod. Plant development rates to visible floral buds and anthesis stagesand phyllochron varied widely amongst accessions and across environments within a short day responsethough phyllochron varied mostly during the reproductive phase. There was a very strong associationbetween time to flowering and altitude of origin (r = &#8722;0.98), mean temperature of the wettest quarter(r = 0.98) and Normalized Difference Vegetation Index values (r = 0.73). Photoperiod sensitivity was higherfor accessions from the lowlands (normally late flowering), while temperature sensitivity was greatestfor accessions from the highlands (early flowering); most variation for these traits detected at the specieslevel was found in North West Argentina. Genotype by environment interactions for yield were relatedto the traits examined in this study and considering their high heritability it is suggested that quinoabreeding programs targeted for specific adaptation to a wide range of environments can be developedfrom this germplasm.","palabras_clave":"Chenopodium quinoa Willd.|Flowering|Phyllochron|Genotype × environment interactiona"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3799,"idioma_id":4,"titulo":"A Modular Pipelined Processor for High Resolution Gamma-Ray Spectroscopy","resumen":"The design of a digital signal processor for gamma-ray applications is presented in which a single ADC input can simultaneously provide temporal and energy characterization of gamma radiation for a wide range of applications. Applying pipelining techniques, the processor is able to manage and synchronize very large volumes of streamed real-time data. Its modular user interface provides a flexible environment for experimental design. The processor can fit in a medium-sized FPGA device operating at ADC sampling frequency, providing an efficient solution for multi-channel applications. Two experiments are presented in order to characterize its temporal and energy resolution.","palabras_clave":"DIGITAL|PULSE|PROCESSING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3800,"idioma_id":4,"titulo":"Vortex matter freezing in Bi2Sr2CaCu2O8 samples with a very dense distribution of columnar defects","resumen":"We study dynamically-frozen vortex configurations in heavy-ion irradiated Bi2Sr2CaCu2O8 samples with a dense distribution of columnar defects corresponding to a matching field B&#934;= 5kG. We analyze magnetic decoration images obtained at diluted vortex densities, B &#8764; 10&#8722;2 B&#934; , frozen at a temperature T freez between the onset of irreversible magnetic behavior and the decoration temperature of 4.2 K. We find amorphous configurations with liquid-like correlations and Gaussiandistributions of the components of the vortex-defect forces. Assuming strongly localized vortices we propose a model that shows that the observed translational order is fairly consistent with a relaxationdynamics dominated by double-kink excitations near the freezing temperature. We predict a T freez of the same order but smaller than the irreversibility and the first-order transition temperatures.We argue that the low-field vortex structures nucleated in a dense distribution of columnar defects correspond to a typical configuration of a non-entangled liquid with strongly reduced mobility rather than to a metastable glassy phase with divergent activation barriers.","palabras_clave":"VORTEX PHASES|MAGNETIC PROPERTIES|SUPERCONDUCTIVITY PHASE DIAGRAMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3801,"idioma_id":4,"titulo":"?REMOVAL OF ARSENIC (V) IONS FROM AQUEOUS MEDIA BY ADSORPTION ON MULTIWALL CARBON NANOTUBES THIN FILM USING XRF TECHNIQUE?.","resumen":"tA solid phase extraction using multiwalled carbon nanotubes, modified with a tertiary amine, ALIQUAT336, has been developed as a preconcentration technique for the determination of trace amounts ofAs(V) in water samples. After 'batch-based mode' As(V) adsorption on ALIQUAT 336-Multiwalled carbonnanotubes, determinations were made directly on the solid by X-Ray Fluorescence spectrometry, whichreduces the number of reagents used and minimizes the sample handling.The method herein described combines the advantages of solid phase extraction with the benefits ofany X-Ray Fluorescence method associated to the large areas held by the carbon nanotubes, which makethem a promising solid sorbent for preconcentration procedures.The enrichment factor was calculated considering that the thin film obtained from the 100 mL solutionof 1000  g L-1As had a final thickness of 0.04 mm and a final diameter of 16.7 mm, the volume depositedon the pellet was 0.0088 cm3. Thus the preconcentration factor obtained was 104fold, which representeda highly satisfactory value for trace analysis by X-Ray Fluorescence.Satisfactory results were obtained for the determination of arsenic in samples of the standard referencematerial 'NIST 1643e?Trace Elements in Water and drinking water'.© 2016 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.","palabras_clave":"Arsenic|Preconcentration ALIQUAT 336|Nanotubes Thin film|X-ray fluorescencea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3803,"idioma_id":4,"titulo":"Ultradeformable archaeosomes for needle free nanovaccination with Leishmania braziliensis antigens","resumen":"Total antigens from Leishmania braziliensis promastigotes, solubilized with sodium cholate(dsLp), were formulated within ultradeformable nanovesicles (dsLp-ultradeformablearchaeosomes, (dsLp-UDA), and dsLp-ultradeformable liposomes (dsLp-UDL)) and topicallyadministered to Balb\/c mice. Ultradeformable nanovesicles can penetrate the intactstratum corneum up to the viable epidermis, with no aid of classical permeation enhancersthat can damage the barrier function of the skin. Briefly, 100 nm unilamellar dsLp-UDA (soybeanphosphatidylcholine: Halorubrum tebenquichense total polar lipids (TPL): sodium cholate,3:3:1 w:w) of -31.45 mV Z potential, containing 4.84 ± 0.53% w\/w protein\/lipid dsLp,235 KPa Young modulus were prepared. In vitro, dsLp-UDA was extensively taken up byJ774A1 and bone marrow derive cells, and the only that induced an immediate secretion ofIL-6, IL-12p40 and TNF-&#945;, followed by IL-1&#946;, by J774A1 cells. Such extensive uptake is akey feature of UDA ascribed to the highly negatively charged archaeolipids of the TPL,which are recognized by a receptor specialized in uptake and not involved in downstreamsignaling. Despite dsLp alone was also immunostimulatory on J774A1 cells, applied twice aweek on consecutive days along 7 weeks on Balb\/c mice, it raised no measurable responseunless associated to UDL or UDA. The highest systemic response, IgGa2 mediated, 1 loglower than im dsLp Al2O3, was elicited by dsLp-UDA. Such findings suggest that in vivo,UDL and UDA acted as penetration enhancers for dsLp, but only dsLp-UDA, owed to itspronounced uptake by APC, succeeded as topical adjuvants. The actual TPL composition,fully made of sn2,3 ether linked saturated archaeolipids, gives the UDA bilayer resistance against chemical, physical and enzymatic attacks that destroy ordinary phospholipids bilayers.Together, these properties make UDA a promising platform for topical drug targeted delivery and vaccination, that may be of help for countries with a deficient healthcaresystem.","palabras_clave":"archeosomes|Leishmania|Young modulus|AFM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3804,"idioma_id":4,"titulo":"ISO 26000 in the post-2015 development agenda","resumen":"In January 2016, the 2030 Agenda for Sustainable Development came into effect.Now, the eyes of the world are on solutions to guide international development and cooperation for the 17 initiatives (goals) over the next 15 years.Can ISO 26000 make a difference?","palabras_clave":"SUSTAINABLE DEVELOPMENT GOALS|AGENDA POST 2015"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3805,"idioma_id":4,"titulo":"Heteroleptic complexes of antifungal drugs with the silver ion","resumen":"AbstractIn spite of a large number of antibiotics and chemotherapeutics available for medical use, the emergence of old and newantibiotic resistance in the last decades revealed a substantial medical need for new classes of antimicrobial agents. In thispaper we report the synthesis, characterization by elemental analysis, Fourier transform infrared and Nuclear magneticresonance spectroscopies, and antifungal properties of two heteroleptic complexes of albendazole (albz) with Ag(I), usingKSCN and o-phenanthroline (phen) as the second ligand, respectively. Both complexes showed a moderate antifungalactivity with all the assayed fungi, mainly Candida albicans and Candida tropicalis, that showed no activity against albendazole.","palabras_clave":"Albendazole|Antifungal properties|Heteroleptic complexes| Silver complexes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3806,"idioma_id":4,"titulo":"The Control of Trichostrongyle Infections in Grazing Cattle of Argentina in a Context of Multiple Anthelmintic Resistances","resumen":"A field program to control multiple anthelmintic resistant worm infections in cattle of Argentina is described. In 2003 around 140 calves died as a consequence of high worm burdens despite the administration of monthly alternate treatments with ivermectin (IVM) or fenbendazole (FBZ). A fecal egg count reduction test (FECRT) showed a clinical efficacy of 73%, 49.1% and 90.4% for IVM, FBZ and levamisole (LVM) respectively. An efficacy controlled test (ECT) showed that efficacy of IVM was 76.1% and 22.6% against Haemonchus spp. and Cooperia spp. respectively; for FBZ efficacy was: 0 % Ostertagia spp.; 28.3% Haemonchus spp. and 24.2% Cooperia spp.A rational control program based on epidemiological surveillance was establishedsince 2004 up to 2012 based on nematode egg excretion and speciation of worms incoprocultures. The LVM was used when necessary.After ten years the results of a FECRT indicated a clinical efficacy of 79% forIVM (Cooperia spp. resistant), 89.4% for FBZ (Ostertagia spp. resistant) and 95.8% for LVM. The ECT showed that efficacy of IVM was &#8805;99% against Haemonchus spp. and Ostertagia spp. and 56% against Cooperia spp. The FBZ showed an efficacy of 100% against Haemonchus spp. and Cooperia spp, and 74% against Ostertagia spp. These findings demonstrate that resistance to IVM and FBZ persisted for Cooperia spp. and Ostertagia spp. respectively despite both compounds had not been used throughout a ten year period.The control programme successfully stopped mortality, minimized subclinical losses and reduced the number of treatments.","palabras_clave":"CATTLE|MULTIPLE RESITANCE|CONTROL|COOPERIA|OSTERTAGIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3807,"idioma_id":4,"titulo":"Contrasting gene expression patterns induced by levodopa and pramipexole treatments in the rat model of Parkinson's disease","resumen":"Whether the treatment of Parkinson´s disease has to be initiated with levodopa or a D2 agonist like pramipexole remains debatable. Levodopa is more potent against symptoms than D2 agonists, but D2 agonists are less prone to induce motor complications and may have neuroprotective effects. Although regulation of plastic changes in striatal circuits may be the key to their different therapeutic potential, the gene expression patterns induced by de novo treatments with levodopa or D2 agonists are currently unknown. By studying the whole striatal transcriptome in a rodent model of early stage Parkinson´s disease, we have identified the gene expression patterns underlying therapeutically comparable chronic treatments with levodopa or pramipexole. Despite the overall relatively small size of mRNA expression changes at the level of individual transcripts, our data show a robust and complete segregation of the transcript expression patterns induced by both treatments. Moreover, transcripts related to oxidative metabolism and mitochondrial function were enriched in levodopa-treated compared to vehicle-treated and pramipexole-treated animals, whereas transcripts related to olfactory transduction pathways were enriched in both treatment groups compared to vehicle-treated animals. Thus, our data reveal the plasticity of genetic striatal networks possibly contributing to the therapeutic effects of the most common initial treatments for Parkinson´s disease, suggesting a role for oxidative stress in the long term complications induced by levodopa and identifying previously overlooked signaling cascades as potentially new therapeutic targets.","palabras_clave":"Parkinson's disease|antiparkinsonian therapy|levodopa|microarrays"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3808,"idioma_id":4,"titulo":"Synthesis and properties of 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl] chlorin as potential broad-spectrum antimicrobial photosensitizers","resumen":"A novel 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]chlorin (TAPC) was synthesized by reduction of the corresponding porphyrin TAPP with p-toluenesulfonhydrazide, followed by selective oxidation with o-chloranil. Spectroscopic properties and the photodynamic activity of these photosensitizers were compared in N,N-dimethylformamide. An increase in the absorption band at 650 nm was found for the chlorin derivative with respect to TAPP. These photosensitizers emit red fluorescence with quantum yields of 0.15. Both compounds were able to photosensitize singlet molecular oxygen with quantum yields of about 0.5. Also, the formation of superoxide anion radical was detected in presence of TAPC or TAPP and NADH. Photodynamic inactivation was investigated on a Gram-positive bacterium Staphylococcus aureus, a Gram-negative bacterium Escherichia coli and a fungal yeast Candida albicans cells. In vitro experiments showed that TAPC or TAPP were rapidly bound to microbial cells at short incubation periods. These photosensitizers, without intrinsic positive charges, contain four basic amino groups. These substituents can be protonated at physiological pH, increasing the interaction with the cell envelopment. Photosensitized inactivation improved with an increase of both photosensitizer concentrations and irradiation times. After 15 min irradiation, a 7 log reduction of S. aureus was found for treated with 1 &#61549;M photosensitizer. Similar result was obtained with E. coli after using 5 &#61549;M photosensitizer and 30 min irradiation. Also, the last conditions produced a decrease of 5 log in C. albicans cells. Therefore, TAPC was highly effective as a broad-spectrum antimicrobial photosensitizer.","palabras_clave":"CHLORIN|PORPHYRIN|PHOTODYNAMIC ACTIVITY|MICROORGANISMS|PHOTOINACTIVATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3809,"idioma_id":4,"titulo":"Antioxidant properties in a non-polar environment of difluoromethyl bioisosteres of methyl hydroxycinnamates","resumen":"Objectives Many natural antioxidants have poor pharmacokinetic properties that impair their therapeutic use. For hydroxycinnamic acids (HCAs) and other phenolic antioxidants, their major drawback is their low lipophilicity and a rapid metabolism. The difluoromethyl group may be considered as a ´lipophilic hydroxyl´ due to its hydrogen bond donor and acceptor properties; this prompted us to assess it as a bioisosteric replacement of a phenolic hydroxyl for increasing the lipophilicity of HCAs. Methods Six difluoromethyl-substituted methyl cinnamates (4a-c, 5a-c) related to caffeic acid were synthesized and their antioxidant activity evaluated by chemical (FRAP, DPPH scavenging, inhibition of &#946;-carotene bleaching, at 1-200 &#956;m), electrochemical (differential pulse voltammetry, cyclic voltammetry) and cell-based (inhibition of lipid peroxidation in erythrocytes, at 1 and 50 &#956;m) assays. Key fndings Analogues 4a-c and 5a-c were inactive in FRAP and DPPH assays and only those containing a free phenolic hydroxyl (4a and 5a) exhibited electrochemical activity although with high redox potentials. Compounds 4a,b and 5a,b were active in the inhibition of &#946;-carotene bleaching assay and all analogues inhibited lipid peroxidation in the human erythrocytes assay. Conclusions Lipophilic difluoromethyl-substituted cinnamic esters retain radical scavenging capabilities that prove useful to confer antioxidant properties in a non-polar environment.","palabras_clave":"ACTIVIDAD ANTIOXIDANTE|GRUPO DIFLUOROMETILO|ACIDOS HIDROXICINAMICOS|PEROXIDACION DE LIPIDOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3811,"idioma_id":4,"titulo":"Distributional Properties of Multivariate Process Capability Indices under Normal and Non-normal Distributions","resumen":"Multivariate capability analysis has been focus of study in recent years, during which many authors have proposed different multivariate capability indices. In the operative context, capability indices are used as measures of the ability of the process to operate according to specifications. Since the numerical value of the index is used to conclude about the capability of the process, it is essential to bear in mind that almost always that value is obtained from a sample of process units. Therefore it is really necessary to know the properties that the indices have when they are calculated on sampling information, in order to assess the goodness of the inferences made from them. In this work, we conduct a simulation study to investigate distributional properties of two existing indices: MCpm due to Shahriari and Abdollahzadeh and Mp2 due to Shinde and Khadse. We analyze the relative bias and the mean square error of the estimators of the indices, and we also obtain their empirical distributions which are used to estimate the probability that the indices classify correctly a process as capable or as incapable. The results allow us to recommend the use of one of these indices, since it has shown better properties.","palabras_clave":"CAPABILITY INDICES|MULTIVARIATE DISTRIBUTIONS|ESTIMATOR PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3812,"idioma_id":5,"titulo":"Obtención de barbados de  Salix humboldtiana Willd. (sauce criollo) destinados a su reintroducción en arroyos  del centro de la provincia de Buenos Aires.","resumen":"En Argentina la especie nativa Salix humboldtiana Willd. presenta un área extensa de distribución que abarca desde Salta, Jujuy y Formosa, en el extremo norte, hasta la Patagonia. En el centro de la provincia de Buenos Aires su presencia se remonta a la época de plena ocupación de los pueblos originarios, pero en la actualidad, dentro de la cuenca del arroyo del Azul la misma es prácticamente nula. Como parte de un proyecto de investigación cuyo objeto es la conservación y difusión del S. humboldtiana en los ambientes asociados a los cursos de agua ubicados en el centro y  sur de la provincia de Buenos Aires, se evaluaron cuatro diferentes tratamientos para la obtención de barbados de un año, aptos  para  dicha finalidad. El material a propagar se obtuvo de una población espontánea de Salix humboldtiana establecida sobre las márgenes del río Sauce Grande (38º18?49.83? S; 61º43?35.38?O), próxima a la localidad de Saldungaray, provincia de Buenos Aires. Se realizó un ensayo factorial 2x2 en un DBCA con 4 repeticiones. Los tratamientos surgieron de la combinación de dos diámetros (mayor a 1 cm y menor a 1 cm) y de dos estados de humectación (con y sin humedad) de estacas previamente a su plantación. La variable respuesta fue el largo de la guía al término de la estación de crecimiento. El ANOVA permitió descartar la ausencia de interacción entre las variables, resultando significativa la variable diámetro de la estaca (p=0,0265).","palabras_clave":"SALIX HUMBOLDTIANA|ESPECIES NATIVAS|REINTRODUCCION|MULTIPLICACION DE SALICACEAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3813,"idioma_id":4,"titulo":"Quiz games as a model for information hiding","resumen":"We present a general computation model inspired in the notion of information hiding in software engineering. This model has the form of a game which we call quiz game. It allows in a uniform way to prove exponential lower bounds for several complexity problems.","palabras_clave":"LOWER COMPLEXITY BOUND|ELIMINATION PROBLEM|GEOMETRICALLY ROBUST|INFORMATION HIDING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3814,"idioma_id":4,"titulo":"Stearoyl-CoA desaturase indexes and n-6\/n-3 fatty acids ratio as biomarkers of cardiometabolic risk factors in normal-weight rabbits fed high fat diets","resumen":"AbstractBackground: Biomarkers for cardiometabolic risk (CMR) factors would be important tools to maximize the effectivenessof dietary interventions to prevent cardiovascular diseases. Thus, the aim of this work was to analyze stearoyl-CoAdesaturase (SCD) indexes and n-6\/n-3 fatty acids (FA) ratio as biomarkers of CMR induced by feeding rabbits onhigh fat diets (HFDs).Methods: Rabbits were fed either regular diet or 18 % fat in regular diet (HFD) or 1 % cholesterol diet (HD) ordiet containing 1 % cholesterol and 18 % fat (HFD-HD) during 6 weeks. Body weights (BW), blood pressure,visceral abdominal fat (VAF) and glucose tolerance test were determined. Total cholesterol (TC), low densitylipoprotein-cholesterol (LDL-C), high density lipoprotein-cholesterol (HDL-C), triglycerides (TG), fasting glucose(FG), and FA levels from plasma were measured. SCD indexes were calculated as product\/precursor ratios ofindividual FA.Results: BW was similar in all diet groups. HD increased TC, LDL-C, HDL-C, and TG. HFD increased TG, VAF andFG, and decreased HDL-C. The addition of HFD to HD joined to dyslipidemia increased VAF and FG. SCD indexeswere increased and n-6\/n-3 was unchanged in HD. SCD indexes were reduced and n-6\/n-3 FA ratio was increased inHFD and HFD-HD. CMR factors were correlated positively with n-6\/n-3 FA ratio. Although VAF had a strongercorrelation with n-6\/n-3 FA ratio than with SCD indexes, VAF was associated independently to both markers.Conclusions: HFD simulating lipid composition of the average Western-style diet induced experimental rabbitmodels of normal-weight metabolic syndrome (MS). SCD indexes and n-6\/n-3 were modified according to thetype of dietary fat. Considering that VAF and CMR factors appear to be stronger associated to n-6\/n-3 FA ratiothan to SCD indexes, n-6\/n-3 FA ratio may be a better biomarker of MS and CMR in normal-weight subjects thanSCD indexes.Keywords: Metabolic syndrome, High fat diet, Stearoyl-coA desaturase, n-6\/n-3 fatty acid ratio, Biomarkers,Visceral abdominal fat* Correspondence: sjerez@herrera.unt.edu.ar1Instituto Superior de Investigaciones Biológicas (INSIBIO, UNT-CONICET), AvIndependencia 1800, San Miguel de Tucumán, Tucumán 4000, Argentina2Facultad de Ciencias Naturales e Instituto Miguel Lillo, Universidad Nacionalde Tucumán (UNT), Miguel Lillo 205, San Miguel de Tucumán, Tucumán4000, ArgentinaFull list of author information is available at the end of the article© 2016","palabras_clave":"METABOLIC SYNDROME|HIGH FAT DIET|STEAROYL-COA DESATURASE|N-6\/N-3 FATTY ACID RATIO|BIOMARKERS|VISCERAL ABDOMINAL FAT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3815,"idioma_id":4,"titulo":"Genotypic variation in response to salinity in a new sexual germplasm of Cenchrus ciliaris L.","resumen":"As part of a breeding program for new salt-tolerant sexual genotypes of Cenchrus ciliaris L., here weevaluated the salt-stress response of two new sexual hybrids, obtained by controlled crosses, at seedlingand germination stages. A seedling hydroponic experiment with 300 mM NaCl was performed andphysiological variables and growth components were evaluated. While salt-treated sexual material didnot show a decrease in productivity with respect to control plants, a differential response in somephysiological characteristics was observed. Sexual hybrid 1-9-1 did not suffer oxidative damage and itsproline content did not differ from that of control treatment. By contrast, sexual hybrid 1-7-11 sufferedoxidative damage and accumulated proline, maintaining its growth under saline stress. At the germinationstage, sexual hybrid 1-9-1 presented the highest Germination Rate Index at the maximum NaClconcentration assayed, suggesting an ecological advantage in this genotype. These new sexual resourcesare promising maternal parental with differential response to salt and could be incorporated in abreeding program of C. ciliaris in the search of new genotypes tolerant to salinity","palabras_clave":"CENCHRUS CILIARIS|SEXUAL HYBRIDS|SALT STRESS|SEEDLING|GERMINATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3816,"idioma_id":4,"titulo":"Adsorption of CsCl on porous SiO2 glass surface: Experimental results and ab-initio calculations","resumen":"The disposal of nuclear wastes is an issue of global concern due to the physicochemical and radiologic properties of some isotopes. Cesium radionuclides exhibit high solubility and some of them pose a long half-life. This work focuses on the study of Cs+ adsorption on the surface of a porous silica glass. Adsorption experiments were done at room temperature using aqueous solutions with concentrations of 0.1 and 0.5 M of CsCl at pH = 5 and surface area to volume of 0.1 cm- 1. In these conditions, 14 and 18 mgCs\/gSiO2 were adsorbed, respectively. The adsorbed quantity of Cs+ was determined by atomic absorption spectroscopy (AAS) analyzing the quantity of Cs+ contained in the solution before and after adsorption experiments. The Cs+\/Cl- ratio at a glass cross section surface, determined by energy dispersive spectroscopy analysis, is near 6. In addition, first principle calculations were performed, in order to determine charge transfer and location of the active sites for Cs+ and Cl-. Adhesion energy values are presented, which explain the experimental values found for the Cs+\/Cl- concentration ratio at the silica glass surface. The parameters calculated from experimental and ab-initio calculations results were used to explain the adsorption mechanism.","palabras_clave":"Sílice porosa|Adsorción"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3817,"idioma_id":4,"titulo":"Anodic Characteristics and Stress Corrosion Cracking Behavior of Nickel Rich Alloys in Bicarbonate and Buffer Solutions","resumen":"tThe aim of this work is to investigate which alloying element in C-22 is responsible for the crackingsusceptibility of the alloy in bicarbonate and two buffer solutions (tungstate and borate). Six nickel basedalloys, with different amount of chromium (Cr) and molybdenum (Mo) were tested using electrochemicalmethods and slow strain rate tests (SSRT) at 90&#9702;C. All Cr containing alloys had transgranular cracking athigh anodic potential; however, C-22 containing high Cr and high Mo was the most susceptible alloy tocracking. Bicarbonate was the most aggressive of three tested environments of similar pH.","palabras_clave":"Alloy C-22, Nickel Alloys|Stress Corrosion Cracking, SSRT|Bicarbonate, Tungstate, Borate|Anodic Peak"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3818,"idioma_id":4,"titulo":"Optimal design and sensitivity analysis of post-combustion CO2 capture process by chemical absorption with amines","resumen":"This paper presents a sensitivity analisys of CO2 capture processes using amines. Optimal processes are obtained using GAMS, solving a set of optimization problems including rigurous models describing the capture process. Critical variables are selected for the parametric analysis.","palabras_clave":"CO2 CAPTURE PROCESSES|OPTIMAL DESIGN|SIMULTANEOUS OPTIMIZATION APPROACH|CHEMICAL ABSORPTION WITH AMINES|SENSITIVITY ANALYSIS|MATHEMATICAL PROGRAMMING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3819,"idioma_id":5,"titulo":"BALANCE DE MASA DE NITROGENO EN EL CULTIVO DE PAPA EN VILLA DOLORES, CORDOBA","resumen":"La utilización de elevadas dosis de nitrógeno (N) en cultivos de papa bajo riego puede incrementar el riesgo de pérdida al ambiente. El objetivo de este trabajo fue evaluar el efecto de la dosis (0, 100, 150 y 250 kg N ha-1), la fuente (urea y urea tratada con nBTPT) y el momento de aplicación (plantación e inicio de tuberización) de N sobre el balance de masa de N en el cultivo de papa en Villa Dolores, Córdoba. Se estimaron las pérdidas de N cuantificando la entrada (N inicial, mineralizado del suelo y en el fertilizante) y la salida de N (N acumulado en tubérculo, en rastrojo y residual en el suelo), en dos lotes de producción. No se determinaron ventajas del nBTPT respecto a la urea convencional en ninguna de las variables evaluadas. El rendimiento de tubérculo fue mayor con la dosis de 250 kg N ha-1, o con 150 y 100 kg N ha-1 en inicio de tuberización. La fertilización con 100 kg N ha-1 fraccionada presentó menor rendimiento respecto a esta dosis aplicada en IT sin fraccionar. El balance de N permitió estimar pérdidas de 29 a 235 kg N ha-1, las que fueron de relevancia inclusive en el tratamiento testigo. La mayor parte de ellas se adjudicó al lavado de nitrato en etapas iniciales del ciclo.","palabras_clave":"Perdida de nitrógeno|nBTPT|papa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3820,"idioma_id":4,"titulo":"Cervical mucus characteristics and hormonal status at insemination of Holstein cows","resumen":"The present study was carried out to characterize the cervical mucus (CM) collected when inseminating Holstein cows and to relate the secretion pattern with pregnancy. The mucus was collected from mid-cervix of 64 cows with spontaneus estrus (SE) and induced estrus (IE). The quantity, pattern and consistency of the mucus, pH, arborization patterns and the sperm motility were observed. The levels of progesterone and estradiol 17&#946; in serum were also determined. Pregnancy detection was performed by means of transrectal ultrasonography 60 days after insemination. Mucous secretion of cows with SE was significantly different from those with IE, showing a lower degree of crystallization (SE 2.00; IE 2.75) and a lower level of steroid hormones (P4: SE 0.17 ng\/ml and IE 0.33 ng\/ml (t 1.99547 &#945; 0.05); E2: SE 30.95 pg\/ml and IE 47.76 pg\/ml (t 1.99495 &#945; 0.05). Progesterone level was significantly lower and estrogen significantly higher in pregnant cows (P) in relation to that observed in non pregnant females (N) (P4: P 0.20 ng\/ml and N 0.44 ng\/ml (t 1.99602 &#945; 0.05); E2: P 54.77 pg\/ml and N 40.75 pg\/ml (t 1.99505 &#945; 0.05). In conclusion pregnancy was associated with acopious, clear and watery discharge (similar to egg white), with an arborization degree of 2.25, presence of atypical fern leaves together with rosette formations and needles or thorns on rails due to low levels of progesterone accompanied by high concentrations of estrogens.","palabras_clave":"Cristalization|Estrogen| Insemination|Pregnancy|Progesterone"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3821,"idioma_id":5,"titulo":"Políticas sociales de distribución de textos escolares: asistencialización y focalización","resumen":"En este artículo se aborda el estudio de las políticas sociales de distribución de textos escolares a través del Plan Social Educativo (PSE) (1). Se sostiene como eje del debate que las reformas educativas ocurridas a partir de la sanción de la Ley Federal de Educación se articulan en la trama de la asistencialización que se sucede en las políticas sociales durante la década de 1990; las cuales pusieron en disputa el modo de construcción de ciudadanía a la par que configuraron nuevas formas de producción y reproducción de la relación Estado? sociedad en pos de garantizar la gobernabilidad. La presentación se organiza en tres apartados. En el primero se analiza el proceso de asistencialización y focalización de las políticas sociales en especial de las políticas educativas. Luego se examinan algunos rasgos relativos a la forma que asume la política de distribución de textos escolares en el marco del PSE. En el tercer apartado, desde los aportes metodológicos de la agenda setting-política, se realiza la revisión global de las noticias vinculadas a esta política social publicadas en la Revista Zona Educativa (de edición mensual) del Ministerio de Cultura y Educación de la Nación.","palabras_clave":"POLÍTICAS SOCIALES|ASISTENCIALIZACIÓN |FOCALIZACION|TEXTOS ESCOLARES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3822,"idioma_id":4,"titulo":"Prototype muon detectors for the AMIGA component of the Pierre Auger Observatory","resumen":"AMIGA (Auger Muons and Infill for the Ground Array) is an upgrade of the Pierre Auger Observatory to extend its range of detection and to directly measure the muon content of the particle showers. It consists of an infill of surface water-Cherenkov detectors accompanied by buried scintillator detectors used for muon counting. The main objectives of the AMIGA engineering array, referred to as the Unitary Cell, are to identify and resolve all engineering issues as well as to understand the muon-number counting uncertainties related to the design of the detector. The mechanical design, fabrication and deployment processes of the muon counters of the Unitary Cell are described in this document. These muon counters modules comprise sealed PVC casings containing plastic scintillation bars, wavelength-shifter optical fibers, 64 pixel photomultiplier tubes, and acquisition electronics. The modules are buried approximately 2.25 m below ground level in order to minimize contamination from electromagnetic shower particles. The mechanical setup, which allows access to the electronics for maintenance, is also described in addition to tests of the modules´ response and integrity. The completed Unitary Cell has measured a number of air showers of which a first analysis of a sample event is included here.","palabras_clave":"DETECTOR DESIGN AND CONSTRUCTION TECHNOLOGIES AND MATERIALS|PARTICLE DETECTORS|OVER- ALL MECHANICS DESIGN (SUPPORT STRUCTURES AND MATERIALS, VIBRATION ANALYSIS ETC)|PERFORMANCE OF HIGH ENERGY PHYSICS DETECTORS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3823,"idioma_id":4,"titulo":"Nanosecond-level time synchronization of autonomous radio detector stations for extensive air showers","resumen":"o exploit the full potential of radio measurements of cosmic-ray air showers at MHz frequencies, a detector timing synchronization within 1 ns is needed. Large distributed radio detector arrays such as the Auger Engineering Radio Array (AERA) rely on timing via the Global Positioning System (GPS) for the synchronization of individual detector station clocks. Unfortunately, GPS timing is expected to have an accuracy no better than about 5 ns. In practice, in particular in AERA, the GPS clocks exhibit drifts on the order of tens of ns. We developed a technique to correct for the GPS drifts, and an independent method used for crosschecks that indeed we reach nanosecond-scale timing accuracy by this correction. First, we operate a 'beacon transmitter' which emits defined sine waves detected by AERA antennas recorded within the physics data. The relative phasing of these sine waves can be used to correct for GPS clock drifts. In addition to this, we observe radio pulses emitted by commercial airplanes, the position of which we determine in real time from Automatic Dependent Surveillance Broadcasts intercepted with a software-defined radio. From the known source location and the measured arrival times of the pulses we determine relative timing offsets between radio detector stations. We demonstrate with a combined analysis that the two methods give a consistent timing calibration with an accuracy of 2 ns or better. Consequently, the beacon method alone can be used in the future to continuously determine and correct for GPS clock drifts in each individual event measured by AERA.","palabras_clave":"PATTERN RECOGNITION|CLUSTER FINDING|CALIBRATION AND FITTING METHODS|TIMING DETECTORS|DETECTOR ALIGNMENT AND CALIBRATION METHODS (LASERS, SOURCES, PARTICLE-BEAMS)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3824,"idioma_id":4,"titulo":"Search for correlations between the arrival directions of IceCube neutrino events and ultrahigh-energy cosmic rays detected by the Pierre Auger Observatory and the Telescope Array","resumen":"This paper presents the results of different searches for correlations between very high-energy neutrino candidates detected by IceCube and the highest-energy cosmic rays measured by the Pierre Auger Observatory and the Telescope Array. We first consider samples of cascade neutrino events and of high-energy neutrino-induced muon tracks, which provided evidence for a neutrino flux of astrophysical origin, and study their cross-correlation with the ultrahigh-energy cosmic ray (UHECR) samples as a function of angular separation. We also study their possible directional correlations using a likelihood method stacking the neutrino arrival directions and adopting different assumptions on the size of the UHECRmagnetic deflections. Finally, we perform another likelihood analysis stacking the UHECR directions and using a sample of through-going muon tracks optimized for neutrino point source searches with sub-degree angular resolution. No indications of correlations at discovery level are obtained for any of the searches performed. The smallest of the p-values comes from the search for correlation between UHECRs with IceCube high-energy cascades, a result that should continue to be monitored.","palabras_clave":"Ultra high energy cosmic rays|neutrino astronomy|ultra high energy cosmic ray experiments|neutrino experiments"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3825,"idioma_id":4,"titulo":"Self dispersible nanocrystals of Albendazole produced by High Pressure Homogenization and Spray Drying","resumen":"Abstract: Albendazole (ABZ) is a broad-spectrum antiparasitic drug used in the treatment of human or animal infections. Although ABZ has shown a high efficacy for repeated doses in monogastric mammals, its low aqueous solubility leads to erratic bioavailability. The aim of this work was to optimize a procedure in order to obtain ABZ self-dispersible nanocrystals (SDNC) by combining high pressure homogenization (HPH) and spray-drying (SD). The material thus obtained was characterized and the variables affecting both the HPH and SD processes were studied. As expected, the homogenizing pressure and number of cycles influenced the final particle size, while the stabilizer concentration had a strong impact on SD output and redispersion of powders upon contact with water. ABZ SDNC were successfully obtained with high process yield and redispersibility. The characteristic peaks of ABZ were clearly identified in the X-ray patterns of the processed samples. A noticeable increase in the dissolution rate was observed in the aqueous environment.","palabras_clave":"Nanocrystals|Albendazole|Spray Drying|High pressure homogenization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3826,"idioma_id":4,"titulo":"Ivermectin-loaded lipid nanocapsules: toward the development of a new antiparasitic delivery system for veterinary applications","resumen":"Abstract Ivermectin (IVM) is probably one of the most widely used antiparasitic drugs worldwide, and its efficacy is well established. However, slight differences in formulation may change the plasma kinetics, the biodistribution, and in consequence, the efficacy of this compound. The present study focuses on the development of a novel nanocarrier for the delivery of lipophilic drugs such as IVM and its potential application in antiparasitic control. Lipid nanocapsules (LNC) were prepared by a new phase inversion procedure and characterized in terms of size, surface potential, encapsulation efficiency, and physical stability. A complement activation assay (CH50) and uptake experiments by THP-1 macrophage cells were used to assess the stealth properties of this nanocarrier in vitro. Finally, a pharmacokinetics and biodistribution study was carried out as a proof of concept after subcutaneous (SC) injection in a rat model. The final IVM-LNC suspension displayed a narrow size distribution and an encapsulation rate higher than 90 % constant over the evaluated time (60 days). Through flow cytometry and blood permanence measurements, it was possible to confirm the ability of these particles to avoid the macrophage uptake.Moreover, the systemic disposition of IVM in the LNC administered by the SC route was higher (p < 0.05) (1367 ng h\/ ml) compared to treatment with a commercial formulation (CF) (1193 ng.h\/ml), but no significant differences in the biodistribution pattern were found. In conclusion, this new carrier seems to be a promising therapeutic approach in antiparasitic control and to delay the appearance of resistance.","palabras_clave":"Drug delivery systems|Ivermectin nanocapsules| Pharmacokinetic|Tissue distribution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3827,"idioma_id":4,"titulo":"Improvement of the texture and quality of cooked gluten-free pasta","resumen":"The effects of egg-proteins and water content on viscoelastic and quality properties of cooked gluten-free pasta were analyzed. Cooking properties included the determination of optimum cooking time (OCT = 10 min), water absorption, cooking loss, and total organic matter. Cutting force and texture profile parameters were also evaluated. Springiness, resilience, and adhesiveness were mainly controlled by the egg-protein content in the dough, while cooked pasta hardness and the plateau modulus were negatively correlated with dough moisture. The obtained results were mathematically modeled, and a multiresponse optimization process was performed using individual desirability functions based on target properties of wheat flour pasta. These functions were combined into a single composite response (global desirability) to determine the precise amount of the different components in the formulation to obtain high quality gluten-free cooked pasta. Optimized dough was prepared, cooked, and the predicted properties were experimentally validated.","palabras_clave":"GLUTEN-FREE PASTA|COOKING QUALITY|VISCOELASTIC BEHAVIOR|OPTIMIZATION|TEXTURAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3828,"idioma_id":4,"titulo":"Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus","resumen":"Previous work has established that the hormone ghrelin engages the hypothalamic?pituitary?adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of -aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)\/NPY neurons. These datasupport the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore,these data suggestthatthe neuronal circuits mediating ghrelin?s orexigenic action vs. its role as a stress signal are anatomically dissociated.","palabras_clave":"Stress |Ghrelin |Orexigenic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3829,"idioma_id":4,"titulo":"Feature extraction based on bio-inspired model for robust emotion recognition (IF 1.271)","resumen":"Emotional state identification is an important issue to achieve more natural speech interactive systems. Ideally, these systems should also be able to work in real environments in which generally exist some kind of noise. Several bio-inspired representations have been applied to artificial systems for speech processing under noise conditions. In this work, an auditory signal representation is used to obtain a novel bio-inspired set of features for emotional speech signals. These characteristics, together with other spectral and prosodic features, are used for emotion recognition under noise conditions. Neural models were trained as classifiers and results were compared to the well-known mel-frequency cepstral coefficients. Results show that using the proposed representations, it is possible to significantly improve the robustness of an emotion recognition system. The results were also validated in a speaker-independent scheme and with two emotional speech corpora.","palabras_clave":"Emotion Recognition|Bioinspired Signal Processing|Computational Inteligence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3830,"idioma_id":4,"titulo":"Individual consistency in the accuracy and distribution of confidence judgments","resumen":"We examine which aspects of the confidence distributions ? its shape, its bias toward higher or lower values, and its ability to distinguish correct from erred trials ? are idiosyncratic of the who (individual specificity), the when (variability across days) and the what (task specificity). Measuring confidence across different sessions of four different perceptual tasks we show that: (1) Confidence distributions are virtually identical when measured in different days for the same subject and the same task, constituting a subjective fingerprint, (2) The capacity of confidence reports to distinguish correct from incorrect responses is only modestly (but significantly) correlated when compared across tasks, (3) Confidence distributions are very similar for tasks that involve different sensory modalities but have similar structure, (4) Confidence accuracy is independent of the mean and width of the confidence distribution, (5) The mean of the confidence distribution (an individual?s confidence bias) constitutes the most efficient indicator to infer a subject?s identity from confidence reports and (6) Confidence bias measured in simple perceptual decisions correlates with an individual?s optimism bias measured with standard questionnaire.","palabras_clave":"Confidence|Perceptual decisions|Metacognitive judgments|Psychophysics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3831,"idioma_id":4,"titulo":"Quaternionic representation of the genetic code","resumen":"A heuristic diagram of the evolution of the standard genetic code is presented. It incorporates, in a way that resembles the energy levels of an atom, the physical notion of broken symmetry and it is consistent with original ideas by Crick on the origin and evolution of the code as well as with the chronological order of appearence of the amino acids along the evolution as inferred from work that mixtures known experimental results with theoretical speculations. Suggested by the diagram we propose a Hamilton quaternions based mathematical representation of the code as it stands now-a-days. The central object in the description is a codon function that assigns to each amino acid an integer quaternion in such a way that the observed code degeneration is preserved. We emphasize the advantages of a quaternionic representation of amino acids taking as an example the folding of proteins. With this aim we propose an algorithm to go from the quaternions sequence to the protein three dimensional structure which can be compared with the corresponding experimental one stored at the Protein Data Bank. In our criterion the mathematical representation of the genetic code in terms of quaternions merits to be taken into account because it describes not only most of the known properties of the genetic code but also opens new perspectives that are mainly derived from the close relationship between quaternions and rotations.","palabras_clave":"GENETIC CODE|BROKEN SYMMETRY|HAMILTON QUATERNIONS|PROTEIN FOLDING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3832,"idioma_id":5,"titulo":"Macrofauna edáfica en ecosistemas naturales y agroecosistemas de la eco- región Esteros del Iberá (Corrientes, Argentina).","resumen":"Se analizó la macrofauna del suelo de cuatro ambientes naturales y agroecosistemas localizados en zonas altas de la eco-región Esteros del Iberá (Corrientes, Argentina) con distintos grados de perturbación, a fin de contribuir a evaluar el impacto de las actividades productivas en este humedal. Se determinó la diversidad, abundancia, densidad y biomasa de los principales grupos y se compararon las comunidades analizadas. Se aplicó el protocolo de Conservación y Manejo Sustentable de la Biodiversidad del Suelo modificado, en cuatro puntos en cada sitio. Se analizó la influencia de distintos factores de perturbación sobre la biota. La macrofauna del suelo estuvo integrada por 19 órdenes de invertebrados, principalmente oligoquetos terrestres y artrópodos, entre los cuales las termitas, hormigas y coleópteros fueron los más abundantes. Las diferencias entre los ensambles analizados se evidenciaron en su composición taxonómica, diversidad, número de órdenes y densidad de los grupos más abundantes, pero ellas no fueron estadísticamente significativas para esta última característica. La mayoría de los individuos se detectaron en el estrato superficial del suelo (0-10 cm) y la biomasa fue más elevada en los pastizales aunque los órdenes de mayor biomasa relativa fueron distintos en cada sitio. Las similitudes entre ambientes agruparon por un lado al cultivo de algodón y al pastizal forestado, y por otro al bosque y al pastizal sometido a pastoreo. Dos de las perturbaciones ambientales analizadas (reemplazo de la vegetación natural y remoción del suelo) influyeron sobre las comunidades macroedáficas. Las hormigas y lombrices resultaron posibles invertebrados bioindicadores en estas comunidades, en tanto que las termitas subterráneas resultaron importantes por su diversidad y biomasa, principalmente en los dos ambientes con mayor grado de perturbación.","palabras_clave":"INVERTEBRADOS|AGROECOSISTEMAS|PERTURBACIONES|ANTRÓPICAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3833,"idioma_id":5,"titulo":"Polarización sociopolítica y percepción del sistema normativo en Venezuela","resumen":"El estudio indaga la percepción que un grupo de venezolanos tiene del sistema normativo, sus repercusiones psicosociales y su relación con la identificación con el colectivo nacional y la autoestima colectiva nacional, así como compara esta percepción según la auto-percepción de clase social de los participantes. La muestra está compuesta por venezolanos de distintos barrios de la ciudad de Caracas. Los resultados indican que hay una mala percepción del sistema normativo asociada a sentimientos negativos y de desmoralización,  menores niveles de identificación con el colectivo nacional y de sentimientos de orgullo nacional. Además, quienes se perciben como integrantes de las clases menos favorecidas tienen una mejor imagen del  sistema normativo.","palabras_clave":"DESMORALIZACION|POLARIZACION POLITICA|NORMAS|TRANSGRESION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3834,"idioma_id":4,"titulo":"Acetylcholinesterase-inhibitor hydrolysates  obtained from ?in vitro? enzymatic hydrolysis of  mannoproteins extracted from different strains  of yeasts","resumen":"In vitro inhibitory activity against acetylcholinesterase(AChE) of peptides obtained by enzymatic hydrolysis of mannoproteins extractedfrom strains of yeasts was investigated. Yeast mannoproteins were extractedfrom strains belonging to the genera Brettanomyces, Candida, Pichia and Saccharomyces isolated from dairy products. They were obtained by heat treatment in citrate bufferand purified by affinity chromatography with concanavalin A. Each purifiedextract was subsequently hydrolysed with proteolytic enzymes (trypsin, pepsin,chymotrypsin and proteinase K) applied individually or in combination, thus generatingsmaller peptides. Inhibitory activity of the latter against AChE wasdetermined. The molecular weight of mannoproteins, determined by SDS-PAGE, wasbetween 6.5 and 30 kDa. As regards AChE inhibition, a preliminary screening ofall hydrolysed extracts was performed, yielding variable results with 59%maximum inhibition. Subsequently, when inhibitory concentration 50 (IC50) wasdetermined, the extracts showed higher inhibitory activity (between 6.75 and12.3 mg mL _1). Resultsshowed that the mannoproteins separated from yeast strains of food origingenerated bioactive peptides by enzymatic hydrolysis, which can be of interestto the manufacturing of food with potential functional properties.","palabras_clave":"ACETYLCHOLINESTERASE|BIOACTIVE PEPTIDES|FUNCTIONAL FOOD|MANNOPROTEINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3835,"idioma_id":4,"titulo":"Association between severe disease course and nephritis with Q222R polymorphism in DNAse I gene among lupus patients: An Argentine multicenter study","resumen":"Background:  Systemic lupuserythematosus is a multifactorial autoimmune disease and the glomerulonephritisis one of the most severe complications, which leads to severe persistentproteinuria, chronic renal failure, and end-stage renal disease. Thismulticenter study investigated the genetic associations of a non-synonymoussingle-nucleotide polymorphism in DNase I with the risk of lupus and itsinfluence on development of nephropathy in an Argentinean population. Methods: Using the Polymerase chain reaction restriction fragment lengthpolymorphism method, the Q222R (+2373A&#8594;G; Gln244Arg) DNase I polymorphismwas studied in 156 systemic lupus erythematosus patients and 170 healthycontrols. Results: Although no significant association between Q222R polymorphism and therisk of systemic lupus erythematosus was found, the presence of the A allelewas associated with an increased risk for the development of nephropathy (p=0.019, OddRatio=2.196, 95 % confidence interval [1.135-4.247]) and a worse diseasecourse [moderate disease course: p=0.006, Odd Ratio=3.250, 95% confidence interval (1.401-7.539);severe disease course: p=0.040, OddRatio=2.339, 95% confidence interval (1.040-5.260)]. Conclusions:  Abetter understanding of the genetic basis of systemic lupus erythematosus will help inthe development of new and more effectives strategies for the treatment of thedisease in the future.","palabras_clave":"LUPUS|ADNasa|AUTOIMMUNITY|SNP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3836,"idioma_id":5,"titulo":"Políticas de investigación y formación de investigadores en la Facultad de Ciencias Humanas (UNSL): propuestas y desafíos","resumen":"A partir de un balance de diversas instancias de debate y experiencias ocurridas durante el período 2013-2015, el presente trabajo propone mapear la situación actual de la investigación en la Facultad de Ciencias Humanas de la Universidad Nacional de San Luis. La producción se organiza en cinco secciones que recorren: 1) algunos obstáculos estructurales en las vías de formación de investigadores; 2) la sistematización de los resultados de la 'Primera Jornada de Encuentro de Docentes, Investigadores, Estudiantes y Graduados de Investigación' (año 2013); 3) algunos datos que procurar informar sobre la situación actual de la investigación en la FCH; 4) la sistematización de las discusiones desarrolladas recientemente en la 'II Jornada de Investigación en la Facultad de Ciencias Humanas' (año 2015); y finalmente 5) ciertas acciones que están en marcha, promovidas por la Secretaría de Ciencia y Técnica - y por otras unidades de gestión de la Facultad-, con el fin de potenciar el fortalecimiento de la investigación. Al ofrecer información para la reflexión y discusión en el seno de la comunidad académica, se procura poner en debate la política de investigación y la formación de los investigadores que se desarrollan en este ámbito específico de la educación superior, afirmando el lugar relevante que poseen las Ciencias Humanas y Sociales.","palabras_clave":"UNIVERSIDAD|POLÍTICAS DE INVESTIGACIÓN|FORMACIÓN DE INVESTIGADORES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3837,"idioma_id":4,"titulo":"Structural Response of Unreinforced Masonry Walls","resumen":"Historical buildings located in seismic regions have URM (unreinforced masonry) walls of considerable width as their main structure. In order to study the structural response of such type of masonry, it is necessary to know its mechanical and elastic characteristics. For such purpose, it is generally necessary to perform destructive and non-destructive tests. Other procedures, such as the study of masonry through numerical models, would make it possible to predict, with close approximation, the response of masonry to different actions. The purpose of this paper is to evaluate the behavior of URM walls subjected to axial and horizontal load by using a finite element model with the Abaqus code. Constitutive laws of materials, bricks and mortar are defined, using two constitutive models of plasticity and damage included in the materials library. The constitutive models used employ information of experimental tests performed on bricks and mortar. The results of an analysis for an URM wall subjected to axial and horizontal load are presented.Responses of walls were obtained in terms of load versus displacement for different constitutive models and for different qualities of bricks and mortar. The two constitutive models used show similar results, particularly in the response waveforms. However, the ultimate capacity values show mean variations of around 30%. The two constitutive models display high dependence upon themechanical characteristics of bricks and mortar.","palabras_clave":"MASONRY|STRUCTURAL BEHAVIOR|PILES|SEISMIC RESIST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3838,"idioma_id":4,"titulo":"Pentameric models as alternative molecular targets for the design of new antiaggregant agents","resumen":"The structure-based drug design has been an extremely useful technique used for searchingand developing of new therapeutic agents in various biological systems. In the case of AD, this approachhas been difficult to implement. Among other several causes, the main problem might be thelack of a specific stable and reliable molecular target. In this paper the results obtained using a pentamericamyloid beta (A&#946;) model as a molecular target are discussed. Our MD simulations have shown that this system isrelatively structured and stable, displaying a lightly conformational flexibility during 2.0 &#956;s of simulation time. This studyallowed us to distinguish characteristic structural features in specific regions of the pentamer which should be taken intoaccount when choosing this model as a molecular target. This represents a clear advantage compared to the monomer ordimer models which are highly flexible structures with large numbers of possible conformers. Using this pentamericmodel we performed two types of studies usually carried out on a molecular target: a virtual screening and the design onstructural basis of new mimetic peptides with antiaggregant properties. Our results indicate that this pentameric modelmight be a good molecular target for these particular studies of molecular modeling. Details about the predictive power ofour virtual screening as well as about the molecular interactions that stabilize the mimetic peptide-pentamer A&#946; complexesare discussed in this paper","palabras_clave":"molecular modelling|virtual screening|antiaggregant compounds|pentameric model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3839,"idioma_id":4,"titulo":"Natural designer solvents for greening analytical chemistry","resumen":"Developing new green solvents is one of the key subjects in Green Chemistry. Ionic liquids and deep eutecticsolvents were discovered as an option to replace organic solvents. However, ionic liquids and deepeutectic solvents (DES) have still some limitations to be applied to real chemical industry. In this sense,a new generation of designer solvents have emerged in the last decade as promising green media. Whenthe compounds that constitute DES are primary metabolites, namely, aminoacids, organic acids, sugars,or choline derivatives, DES are called Natural Deep Eutectic Solvents (NADES). NADES fully represent greenchemistry principles. These solvents offer many striking advantages including biodegradability, low toxicity,solute stabilization, sustainability, low costs and simple preparation. Thus, interesting applicationsin health-related areas can be proposed. This review presents an overview in order to up-to-date knowledgeregarding NADES with special emphasis on their analytical applications and further perspectivesas truly green solvents.","palabras_clave":"Natural deep eutectic solvents| Green analytical chemistry|Sample preparation| Green solvents"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3840,"idioma_id":4,"titulo":"FISH and AgNor-mapping of the 45S and 5S rRNA genes in wild and cultivated Capsicum species (Solananceae)","resumen":"Chromosome number and position of rDNA were studied in 12 wild and cultivated species of the genus Capsicum with chromosome numbers x = 12 and x = 13 (22 samples). For the first time in these species, the 5S and 45S rRNA loci were localized and physically mapped using two-color fluorescence in situ hybridization and AgNOR banding. We focused on the comparison of the results obtained with both methods with the aim of accurately revealing the real functional rRNA genes. The analyzes were based on a previous work that reported that the 18S-5.8S-25S loci mostly coincide with GC-rich heterochromatic regions and likely have given rise to satellite DNAs, which are not active genes. These data show the variability of rDNA within karyotypes of the genus Capsicum, providing anchor points for (comparative) genetic maps. In addition, the obtained information might be useful for studies on evolution of repetitive DNA.","palabras_clave":"citogenetica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3841,"idioma_id":4,"titulo":"Shallow groundwater dynamics in the Pampas: climate, landscape and crop choice effects","resumen":"Depending on its depth from the soil surface, shallow groundwater can represent a valuable water resource to alleviate droughts, or a stress agent that causes waterlogging and flooding in rainfed crops. Groundwater depth varies across space, following landscape topographic features; through time, accompanying climate fluctuations; and may also shift in both dimensions in response to crop choice. We evaluated the contribution of climate, topography and crop choice on the variability of groundwater depth in rainfed systems of western Pampas, throughout a five year period of extreme precipitation fluctuation (2008?2013). Sixteen permanent monitoring wells were installed in four different topographic settings along the smoothly rolling landscape, covering the three phases of a maize?soybean?wheat\/soybean rotation, common in the region. Water table dynamics, measured at weekly to monthly intervals, was very similar across landscape positions, with a range of depth from the surface of &#8722;0.2 (flood) to 1.8 m and 1.8?4.4 m in the lowest and highest positions, respectively. At the inter-annual scale, water table fluctuations were predominantly dictated by climate variability with no effect due to the implanted crop. Only at the intra-annual scale, crop choice appeared as a relevant control, with wheat?soybean flattening the spring level rises and summer drops repeatedly found under maize and soybean single crops. Daily meteorological data and remote sensing estimates of live and dead crop cover were used to simulate transpiration demand and soil evaporation. As the balance between precipitation and crop evapotranspiration was positive\/negative, watertables raised\/dropped 0.21 cm mm&#8722;1 (n = 80, R2 0.32) and 0.22 cm mm&#8722;1 (n = 1092, R2 0.31) at inter and intra-annual scales, respectively. While crop choice may influence water table levels within a growing season, it has only a subtle effect on year to year fluctuation. With the explored annual crop options, farmers in the Pampas could reduce spring flooding risk when sowing double crops but cannot have a substantial effect on the longer term dynamics of the water table.","palabras_clave":"WATER TABLE DEPTH|ECOHYDROLOGY|ANNUAL CROPS|FARMING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3842,"idioma_id":4,"titulo":"Influence of environmental parameters on mycotoxin production by Alternaria arborescens","resumen":"Alternaria arborescens has been reported as a common fungalspecies invading tomatoes and is capable of producing several mycotoxins ininfected plants, fruits and in agricultural commodities. Alternariol (AOH), alternariolmonomethyl ether (AME), and tenuazonic acid (TeA) are some of the main Alternariamycotoxins that can be found as contaminants of food. This species can producethese toxic metabolites together with AAL toxins (Alternaria alternata f. sp. lycopersicumtoxins), which can act as inhibitors of sphingolipid biosynthesis. Theobjective of this study was to determine the effect of water activity (aw,0.995, 0.975, 0.950) and temperatura (6, 15, 20, 25 and 30 °C) on mycotoxinproduction by A. arborescens on a synthetic tomato medium. The optimum productionof AOH and AME occurred at 0.975 aw after 40 days of incubation at 30 °C. The maximumTeA accumulation was observed at 0.975 aw and 25 °C and at 0.950 aw and 30 °C.AAL TA was produced in higher quantities at 0.995 aw and 30 °C. At 6 °C noquantifiable levels of AOH or AME were detected, but significant amounts of TeAwere produced at 0.975 aw. In general, high aw levels and high temperatureswere favorable for mycotoxin production. The greatest accumulation of all fourtoxins occurred at 0.975 aw and 30 °C. The results obtained here could beextrapolated to evaluate the risk of tomato fruits and tomato productscontamination caused by these toxins.","palabras_clave":"ALTERNARIA ARBORESCENS|ALTERNARIOL|ALTERNARIOL MONOMETHYL ETHER|TENUAZONIC ACID|AAL TOXINS|ENVIRONMENTAL PARAMETERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3843,"idioma_id":4,"titulo":"Sedimentology and alluvial architecture of the Bajo Barreal Formation (Upper Cretaceous) in the Golfo San Jorge Basin: outcrop analogues of the richest oil-bearing fluvial succession in Argentina","resumen":"This study addresses the sedimentology, architecture and dimensions of fluvial deposits in the Bajo Barreal Formation (Upper Cretaceous) in the Codo del Senguerr anticline, Golfo San Jorge Basin, Argentina. The stratigraphic framework of the 450e650 m thick alluvial succession was carried out through description and interpretation using 18 detailed sedimentary logs along a 4.5 km wide exposure, where 314 fluvial channels were analysed. The Bajo Barreal Formation is a high-accommodation fluvial succession, dominated by fine-grained floodplain deposits, with isolated-to-vertically stacked, sheet-like,low-sinuosity or braided fluvial channels of limited lateral mobility clustered in several coeval channel belts, in which fluvial channels were relocated by avulsion. The Lower Member consists of reworked ashfall materials preserved in floodplain areas (78%), floodplain sandstones (4%) and fluvial channels (18%).The mean thickness of sandbodies is 2.96 m (n ¼ 118) and mean true width is 112 m (mean W:T ¼ 43), with thicker and wider sandbodies in upper levels of the Member. The Upper Member consists of grey siltstones and mudstones preserved in extensive lowland areas (78%) and fluvial channels (19%), with scarce preservation of sandstones in the proximal floodplain (3%). Fluvial channels are narrow lowsinuosity sheets with comparable thickness (mean ¼ 3.21 m, n ¼ 196), but greater width than those of the Lower Member (mean width ¼ 147 m, mean W:T ¼ 50). Paleoflow data from 298 fluvial sandbodies indicates a paleoflow direction toward the SE (Az. 112). Rivers flowed parallel to inherited earlyCretaceous normal faults and are oriented to high-angle (&gt;70) to the current axis of the Codo del Senguerr anticline, suggesting that the uplift of the anticline occurred after the deposition of the formation.The variation in geometry of fluvial channels in both Members of the Bajo Barreal Formation could help in planning and developing primary and\/or enhanced oil recovery projects in nearby oilfields, and provide data necessary for modelling the subsurface connectivity of hydrocarbon reservoirs.","palabras_clave":"ALLUVIAL SEDIMENTOLOGY|OUTCROP ANALOGS|HIGH-ACCOMMODATION FLUVIAL SYSTEMS|CHANNEL DIMENSIONS|CRETACEOUS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3844,"idioma_id":5,"titulo":"LA RELACIÓN DE LA NTE PARA LA PREVENCIÓN DE LA VIOLENCIA INTRAFAMILIAR Y LAS FORMAS DE ARTICULACIÓN SOCIAL. APORTES PARA EL DIAGNÓSTICO Y LA EJECUCIÓN DE ESTRATEGIAS DE INTERVENCIÓN","resumen":"La Nueva Teoría Estratégica es el principal punto de partida para la investigación llevada adelante por nuestro equipo. La aproximación al tema de la violencia intrafamiliar surge a partir de un cruce transdisciplinar entre espacios de Comunicación,  Trabajo Social y Educación en el marco de una investigación llevada adelante desde una Universidad Pública del Conurbano Bonaerense de la República Argentina, con antecedentes en investigaciones anteriores (Programa Estrategias y Medios de Comunicación en Internet), situada desde el año 2013 al 2016. Este abordaje se realiza desde el paradigma de la complejidad y cuenta con antecedentes de variadas experiencias de aplicación de la Comunicación Estratégica.En relación al tema de la violencia intrafamiliar hemos establecido cómo ésta deviene como producto de un ecosistema que abarca no solo las conductas individuales. La superposición y complementación de los factores que se presentan en diversos niveles, permite entender las múltiples causas de la violencia y la interacción de los  factores de riesgo que operan en las personas, sus relaciones, en la comunidad y en los ámbitos social, cultural e histórico.  Partiendo de la premisa de que la formación de ciudadanos empoderados y comprometidos en el desarrollo de sus propias comunidades involucra necesariamente el ámbito educativo, este equipo comparte  su acción en el ámbito áulico. Como metodología se han propuesto diversos momentos:  el análisis de piezas comunicativas y protocolos oficiales, la aplicación del modelo RAPC ( Radiografía del Patrón de Conectividad) la elección de subtemas para su abordaje, presentación de recursos y herramientas, puesta en común y evaluación. Se presentan sintéticamente  los resultados de estrategias de comunicación-educación llevadas adelante como transferencia de investigación en el tema. Como elemento emergente durante el período de la investigación surgió una convocatoria que dio lugar a una amplia movilización bajo el lema ?Ni una menos?. Como situación generada a partir de una acción comunicacional muy clara nos resulta interesante poder analizar este hecho que se constituyó como un ejemplo de manifestación de cohesión social a nivel nacional en todo el territorio argentino en el mes de junio de 2015.La estrategia considerada desde su orientación articuladora, centrada en la búsqueda del diálogo, o la comunicación para escuchar y comprender al otro, para encontrar lo que nos une y el ser relacional o articulador social ha sido el eje que nos ha permitido encarar esta investigación y realizar  las consiguientes transferencias.  La Comunicación Estratégica,  se toma como punto de partida,  como una forma de acción social que del conflicto a la articulación social se caracteriza por la interacción simbólica, para resolver problemas o maximizar oportunidades.","palabras_clave":"VIOLENCIA INTRAFAMILIAR|COMUNICACIÓN-EDUCACIÓN|EDUCACIÓN SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3845,"idioma_id":5,"titulo":"FUsAM: Framework para la medición de Usabilidad en Aplicaciones Móviles basado en una SPL","resumen":"El uso masivo de los dispositivos móviles asociado a la heterogeneidad de estos y también de los usuarios implica un desafío al momento de evaluar la usabilidad de las aplicaciones móviles. Es importante disponer de metodologías y herramientas que permitan realizar estudios de usabilidad específicos para este tipo de aplicaciones en las cuales el contexto de uso cambia continuamente. Es necesario que las pruebas de usabilidad sean transparentes para el usuario, recolecten datos  de usabilidad  y del contexto de manera automática, no sean intrusivas para la aplicación que se prueba y que soporten en alguna medida la variabilidad de los dispositivos. Para lograr las características mencionadas,   en este trabajo se propone un framework denominado FUsaM (Framework de Usabilidad Móvil) que es extensible y permite generar e integrar pruebas de   usabilidad en aplicaciones móviles. Para su diseño e implementación se utiliza el enfoque de construcción de Línea de Productos de Software (SPL, Software Product Line) combinado con la Programación Orientada a Características (FOP, Feature-Oriented Programming) y la Programación Orientada a Aspectos (AOP, Aspect-Oriented  Programming). También se presenta un caso de estudio para demostrar la funcionalidad del mismo.","palabras_clave":"APLICACIONES MOVILES|usabilidad|spl"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3846,"idioma_id":5,"titulo":"'Revisión crítica de las concepciones binarias en los procesos de subjetivación contemporáneos'","resumen":"Este artículo corresponde a un recorte de una investigación más amplia que indaga la subjetividad femenina desde la teoría psicoanalítica y los estudios de género en el contexto cultural actual. La articulación entre ambos permite pensar ciertos desafíos que se presentan en la clínica de hoy. Se intenta realizar un análisis crítico de la vigencia de la bipartición identitaria de los géneros para poder comprender las prácticas de la sexualidad. Abordar la sexualidad como experiencia de dimensión sociohistórica implica poner en cuestión la correlación dentro de una cultura entre los campos de saber, los  tipos de normatividad que se establecen, las prácticas eróticas que se visibilizan y las formas de subjetividad que se construyen.","palabras_clave":"PSICOANALISIS|ESTUDIOS DE GENERO |CONCEPCIONES BINARIAS |PROCESOS DE SUBJETIVACION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3847,"idioma_id":4,"titulo":"Temporal Variation of the Current Sheet Inductance from PACO Plasma Focus Device","resumen":"The temporal variation of the current sheet (CS) inductance in a plasma focus device can be calculated using the current derivative and the voltage signal acquired on the anode electrode, which are very common measurements in this type of device. The value of that inductance contains important information about the discharge performed including the CS lift-off from the insulator, voltage between the pinch extremes, maximum energy of the X-ray, energy delivered to the pinch and information about the actuating fusion mechanisms if the filling pressure is deuterium. This work discusses the values of the CS inductance extracted from several discharges of the Plasma Auto Confinado (PACO) plasma focus, installed in the National University of the Center of Buenos Aires, Argentina (2 kJ total energy, capacitor bank of 4l F charged to 31 kV and a maximum current of 250 kA).","palabras_clave":"Plasma Focus|Inductance|Measutement"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3848,"idioma_id":4,"titulo":"Loss of helminth species diversity in the large hairy armadillo Chaetophractus villosus on the Tierra del Fuego Island, Argentina","resumen":"The aim of this work is to compare taxonomic diversity of parasite species of the large hairy armadillo, Chaetophractus villosus in its native range and in another recently introduced population (Tierra del Fuego island), and to evaluate if the isolation of the latter determines a decrease in its parasitic diversity. Forty specimens from Buenos Aires and Tierra del Fuego Provinces were collected and examined for helminths. Eleven parasite species were found in the native population, and only one species was present in Tierra del Fuego (Trichohelix tuberculata). This may be explained because isolation and climatic conditions prevent encounters between potential host species and infective forms of parasites. Further sampling will be needed throughout the entire Patagonia steppe to confirm how the characteristic parasitic fauna of C. villosus behaves across the armadillo?s southern distribution.","palabras_clave":"HELMINTH|CHAETOPHRACTUS VILLOSUS|TIERRA DEL FUEGO|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3849,"idioma_id":4,"titulo":"Synthesis and characterization of an antibacterial powder based on the covalent bonding of aminosilane-stabilized silver nanoparticles to a colloidal silica","resumen":"Stable dispersions of silver nanoparticles (Ag NPs) were synthesized employing glycerol as both a solvent and reducing agent, and 3-aminopropyl trimethoxysilane (APTMS) as a stabilizer. Average sizes varied between 13 and 55 nm, depending on the molar ratio of APTMS\/Ag. Terminal alkoxysilanes reacted with OH groups of glycerol leading to the covalent bonding of glycerol moieties to the chain ends of the stabilizer. This produced extremely stable colloidal dispersions from which NPs could not be extracted with solvents immiscible with glycerol (as THF). Ag NPs were covalently bonded to the surface of a colloidal silica by hydrolysis\/condensation of terminal Si-O-C bonds of the stabilizer with superficial SiOH bonds of silica. TEM images revealed the presence of individual NPs and small clusters of NPs attached to the silica surface. These clusters were presumably generated by intermolecular reactions among chain ends of the stabilizer producing Si?O?Si bonds. The antibacterial properties of the resulting powder were confirmed by conventional tests employing a culture of Escherichia Coli.","palabras_clave":"SILVER NANOPARTICLES|SOL-GEL PROCESS|ANTIBACTERIAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3850,"idioma_id":4,"titulo":"Neuroprotective effect of melatonin in experimental optic neuritis in rats","resumen":"J Pineal Res. 2016 Apr;60(3):360-72. doi: 10.1111\/jpi.12318. Epub  2016 Mar 2.Neuroprotective effect of melatonin in experimental optic neuritis in rats.Aranda ML1, González Fleitas MF1, De Laurentiis A2, Keller Sarmiento MI1, Chianelli M1, Sande PH1, Dorfman D1, Rosenstein RE1.Author informationAbstractOptic neuritis (ON) is an inflammatory, demyelinating, and neurodegenerative condition of the optic nerve, which might induce permanent vision loss. Currently, there are no effective therapies for this disorder. We have developed an experimental model of primary ON in rats through a single microinjection of 4.5 &#956;g of bacterial lipopolysaccharide (LPS) into the optic nerve. Since melatonin acts as a pleiotropic therapeutic agent in various neurodegenerative diseases, we analyzed the effect of melatonin on LPS-induced ON. For this purpose, LPS or vehicle were injected into the optic nerve from adult male Wistar rats. One group of animals received a subcutaneous pellet of 20 mg melatonin at 24 hr before vehicle or LPS injection, and another group was submitted to a sham procedure. Melatonin completely prevented the decrease in visual evoked potentials (VEPs), and pupil light reflex (PLR), and preserved anterograde transport of cholera toxin &#946;-subunit from the retina to the superior colliculus. Moreover, melatonin prevented microglial reactivity (ED1-immunoreactivity, P &lt; 0.01), astrocytosis (glial fibrillary acid protein-immunostaining, P &lt; 0.05), demyelination (luxol fast blue staining, P &lt; 0.01), and axon (toluidine blue staining, P &lt; 0.01) and retinal ganglion cell (Brn3a-immunoreactivity, P &lt; 0.01) loss, induced by LPS. Melatonin completely prevented the increase in nitric oxide synthase 2, cyclooxygenase-2 levels (Western blot) and TNF&#945; levels, and partly prevented lipid peroxidation induced by experimental ON. When the pellet of melatonin was implanted at 4 days postinjection of LPS, it completely reversed the decrease in VEPs and PLR. These data suggest that melatonin could be a promising candidate for ON treatment. © 2016 John Wiley &amp; Sons A\/S. Published by John Wiley &amp; Sons Ltd.","palabras_clave":"axoglial alterations|melatonin|optic neuritis|pupil light reflex|retinal ganglion cells|visual evoked potentials"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3851,"idioma_id":5,"titulo":"Propiedades fisicoquímicas y antibacteriales de mezclas PLA-Quitosano obtenidas por casting con potencial uso como acolchados agrícolas","resumen":"En este trabajo se obtuvieron films híbridos PLA-Quitosano compatibilizados con diisocianato de hexametileno (HDI) por el método de casting y posterior prensado. Los films fueron preparados con diferentes contenidos de quitosano (CS) (0, 10, 20 y 30% en peso) y a continuación se estudiaron sus propiedades fisicoquímicas, mediante espectroscopia infrarroja de Transformada de Fourier (FTIR), análisis termogravimétrico (TGA), calorimetría diferencial de barrido (DSC), microscopia óptica (MO) y difracción de rayos X (DRX). Se encontró que el agente compatibilizante reacciona completamente. Sin embargo, la morfología de las mezclas resultó heterogénea.  En relación a las propiedades fisicoquímicas de los films obtenidos se comprobó que el quitosano afecta sus propiedades térmicas disminuyendo sus temperaturas de descomposición, transición vítrea (Tg) y de fusión (Tf) e incrementando su cristalinidad. Desde el punto de vista funcional, se estudiaron las propiedades antimicrobianas de cada uno de los films frente a la bacteria patógena Pseudomonas syringae pv tomato DC-3000, la cual es el agente causal de la mancha del tomate. El aumento en el contenido de quitosano fue proporcional al efecto antimicrobiano permitiendo proyectar su potencial de aplicación en el campo agronómico.","palabras_clave":"Biodegradable films|Polymeric blends|Casting|Mulching"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3852,"idioma_id":4,"titulo":"Necessary and Sufficient Conditions for the Existence of  Periodic Solutions of a Nicholson Type Delay System","resumen":"We consider a Nicholson type system for two species with mutualism and nonlinear harvesting terms. We give sufficient conditions for the existence of a positive periodicsolution. We also provide a necessary condition; more precisely, we prove that if the harvesting rate is large enough, then 0 is a global attractor for the positive solutions and, in particular,positive periodic solutions cannot exist.","palabras_clave":"NICHOLSON TYPE SYSTEM|NONLINEAR HARVESTING|DELAY DIFFERENTIAL SYSTEMS|POSITIVE PERIODIC SOLUTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3853,"idioma_id":4,"titulo":"Is aridity restricting deforestation and land uses in the South American Dry Chaco?","resumen":"In this paper, we explored how aridity influences the regional deforestation and landuse patterns (i.e. crops\/pastures) in South American Dry Chaco. To do this, we contrasted land use during last decade (2001?2012) with a spatially explicit aridity index, which we complemented with a crop water balance model. Land-use classifications were performed by considering the temporal variability of NDVI from MODIS satellites, showing that 40 and 60% of deforested land was assigned to crops and pastures, respectively. Results indicate that although the regional deforestation pattern was not associated with the aridity gradient, with drier areas similarly deforested as wetter areas, contrasting differences were observed in the use of this land, with crops mostly located (90%) in wetter areas and pastures evenly distributed across the whole aridity gradient. This research highlighted the strong effect of water limitations on the land-use option after deforestation and may help to set the basis for future land-use planning policies.","palabras_clave":"drought|deforestation|agriculture expansion|crops; pastures|land-cover change"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3854,"idioma_id":4,"titulo":"Cystic echinococcosis in South America: systematic review of species and genotypes of Echinococcus granulosus sensu lato in humans and natural domestic hosts","resumen":" objective To systematically review publications on Echinococcus granulosus sensu lato species\/genotypes reported in domestic intermediate and de&#64257;nitive hosts in South America and in humancases worldwide, taking into account those articles where DNA sequencing was performed; and toanalyse the density of each type of livestock that can act as intermediate host, and features of medicalimportance such as cyst organ location.methods Literature search in numerous databases. We included only articles where samples weregenotyped by sequencing since to date it is the most accurate method to unambiguously identify allE. granulosus s. l. genotypes. Also, we report new E. granulosus s. l. samples from Argentina andUruguay analysed by sequencing of cox1 gene.results In South America, &#64257;ve countries have cystic echinococcosis cases for which sequencing dataare available: Argentina, Brazil, Chile, Peru and Uruguay, adding up 1534 cases. E. granulosus s. s.(G1) accounts for most of the global burden of human and livestock cases. Also, E. canadensis (G6)plays a signi&#64257;cant role in human cystic echinococcosis. Likewise, worldwide analysis of human casesshowed that 72.9% are caused by E. granulosus s. s. (G1) and 12.2% and 9.6% by E. canadensis G6and G7, respectively.conclusions E. granulosus s. s. (G1) accounts for most of the global burden followed byE. canadensis (G6 and G7) in South America and worldwide. This information should be taken intoaccount to suit local cystic echinococcosis control and prevention programmes according to eachmolecular epidemiological situation.","palabras_clave":"Echinococcus granulosus sensu lato|genotypes|cystic echinococcosis|South America|neglected disease"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3855,"idioma_id":4,"titulo":"Weighted Best Local Approximation","resumen":"The notion of a best&nbsp; multipoint local approximations of a functionis fully treated in cite{CDR84} where the $L^p$ norm is used.Later, other approaches to best multipoint local approximations with$L^p$ norms appeared in cite{M86} and cite{AW81}. And finally, forOrlicz norms, we mention cite{F94}, cite{CLR04}, cite{FR08},cite{CLMR11}, cite{CLR12} and for a general family of normscite{CLR07}, cite{CZ07} and cite{MZ84}. However, in cite{CDR84},Chui et al. introduced the concept of balanced points in $L^{p}$which includes different importance in each point.","palabras_clave":"Best Local Approximations|Balanced best approximations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3856,"idioma_id":4,"titulo":"Best L2 Local Approximation on Two Small Intervals","resumen":"In this article, we introduce the &#964;-condition, which is weaker thanthe L2 di&#1048576;erentiability. If a function satises the &#964;- condition ontwo points of R, we prove the existence and characterization ofthe best local polynomial approximation on these points.","palabras_clave":"BEST|LOCAL|APPROXIMATION|TWO|POINTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3857,"idioma_id":4,"titulo":"Comprehensive screening for naturally-occurring Hepatitis C virus resistance to direct-acting antivirals in the NS3, NS5A and NS5B genes in worldwide isolates from viral genotypes 1-6.","resumen":"ThereisnocomprehensivestudyavailableonthenaturalhepatitisCvirus(HCV)polymorphisminsitesassociatedwithresistanceincludingallviralgenotypeswhichmaypresentvariablesusceptibilitiestoparticulardirect-actingantivirals(DAAs).This studyaimedtoanalyzethefrequencies,geneticbarriers,andevolutionaryhistoriesofnaturallyoccurringresistance-associated variants(RAVs)inthesixmainHCVgenotypes.Acomprehensiveanalysisofupto103RAVswasperformedin2,901,2,216,and 1,344HCVisolatesfortheNS3,NS5A,andNS5Bgenes,respectively.Wereportsigni&#64257;cantintergenotypicdifferencesinthefrequenciesofnaturalRAVsforthesethreeHCVgenes.Inaddition,wefoundalowgeneticbarrierforthegenerationofnewRAVs, irrespectiveoftheviralgenotype.Furthermore,in1,126HCVgenomes,includingsequencesspanningthethreegenes,haplotype analysisrevealedaremarkablyhighfrequencyofvirusescarryingmorethanonenaturalRAVtoDAAs(53%ofHCV-1a,28.5% ofHCV-1b,67.1%ofHCV-6,and100%ofgenotype2,3,4,and5haplotypes).WiththeexceptionofHCV-1a,themostprevalent haplotypesshowedRAVsinatleasttwodifferentviralgenes.Finally,evolutionaryanalysesrevealedthat,whilemostnatural RAVsappearedrecently,othershavebeenef&#64257;cientlytransmittedovertimeandclusterinwell-supportedclades.Insummary, anddespitetheobservedhighef&#64257;cacyofDAA-basedregimens,weshowthatnaturallyoccurringRAVsarecommoninallHCV genotypesandthatthereisanoveralllowgeneticbarrierfortheselectionofresistancemutations.Thereisaneedfornatural DAAresistancepro&#64257;lingspeci&#64257;cforeachHCVgenotype.","palabras_clave":"HEPATITIS C VIRUS|RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3858,"idioma_id":5,"titulo":"La educación y los organismos internacionales de crédito. Préstamos y recomendaciones para América Latina (2000-2015)","resumen":"El trabajo aborda los temas prioritarios y las estrategias que subyacen en los proyectos financiados por el Banco Mundial y el Banco Interamericano de Desarrollo en Argentina, Brasil, Chile y México, a partir del año 2000 y hasta la actualidad. Desde una perspectiva histórica, se analiza su papel en las reformas educacionales de América Latina de los ?90 y las nuevas orientaciones adoptadas en la etapa más reciente. El análisis de los proyectos permite afirmar que, a pesar de las especificidades del financiamiento en cada país, por parte de cada entidad, existen ciertas líneas de continuidad que son presentadas como solución técnica a replicar frente a viejos y nuevos problemas de los sistemas educativos. El interés en la educación desde la perspectiva de la Teoría del Capital Humano, la búsqueda de la mejora de la productividad y ?empleabilidad? de los trabajadores y el mantenimiento del orden social orientan fuertemente la acción de ambos bancos en el ámbito de la educación.","palabras_clave":"Banco Mundial|BID|Reformas educativas|América Latina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3859,"idioma_id":4,"titulo":"VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model","resumen":"Successful embryo implantation occurs followed by a local pro-inflammatory response subsequently shifted toward a tolerogenic one. VIP (vasoactive intestinal peptide) has embryotrofic, anti-inflammatory and tolerogenic effects. In this sense, we investigated whether the in vivo treatment with VIP contributes to an immunosuppressant local microenvironment associated with an improved pregnancy outcome in the CBA\/J&#8201;×&#8201;DBA\/2 resorption prone model. Pregnancy induced the expression of VIP, VPAC1 and VPAC2 in the uterus from CBA\/J&#8201;×&#8201;DBA\/2 mating females on day 8.5 of gestation compared with non-pregnant mice. VIP treatment (2&#8201;nmol\/mouse i.p.) on day 6.5 significantly increased the number of viable implantation sites and improved the asymmetric distribution of implanted embryos. This effect was accompanied by a decrease in ROR&#947;t and an increase in TGF-&#946; and PPAR&#947; expression at the implantation sites. Moreover, VIP modulated the maternal peritoneal macrophages efferocytosis ability, tested using latex beads-FITC or apoptotic thymocytes, displaying an increased frequency of IL-10-producer F4\/80 cells while did not modulate TNF-&#945; and IL-12 secretion. The present data suggest that VIP treatment increases the number of viable embryos associated with an increase in the efferocytic ability of maternal macrophages which is related to an immunosuppressant microenvironment.","palabras_clave":"PHAGOCYTOSIS|VIP|MACROPHAGES|PREGNANCY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3860,"idioma_id":4,"titulo":"Unsupervised Classification Algorithm Based on EM Method for Polarimetric SAR Images","resumen":"In this work we develop an iterative classification algorithm using complex Gaussian mixture models for the polarimetric complex SAR data. It is a non supervised algorithm which does not require training data or an initial set of classes. Additionally, it determines the model order from data, which allows representing data structure with minimum complexity. The algorithm consists of four steps: initialization, model selection, refinement and smoothing. After a simple initialization stage, the EM algorithm is iteratively applied in the model selection step to compute the model order and an initial classification for the refinement step. The refinement step uses Classification EM (CEM) to reach the final classification and the smoothing stage improves the results by means of non-linear filtering. The algorithm is applied to both simulated and real Single Look Complex data of the EMISAR mission and compared with the Wishart classification method. We use confusion matrix and kappa statistic to make the comparison for simulated data whose ground-truth is known. We apply Davies-Bouldin index to compare both classifications for real data. The results obtained for both types of data validate our algorithm and show that its performance is comparable to Wishart´s in terms of classification quality.","palabras_clave":"SAR images|Classification|Expectation-Maximization|Mixture reduction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3861,"idioma_id":5,"titulo":"Parametrización del modelo CROPGRO-soybean y su uso como herramienta para evaluar el impacto del cambio climático sobre el cultivo de soja","resumen":"Antes de su uso para la toma de decisiones, los modelos de cultivos deben ser calibrados con datos de campo de la región en la cual el modelo será utilizado. El objetivo de este trabajo fue calibrar y validar la capacidad predictiva del modelo CROPGRO-soybean con dos cultivares del grupo de madurez IV (Asgrow 4656 y Don Mario DM4700) en Azul, BA, Argentina, trabajando con datos recogidos en tres años de experimentos de campo en los cuales el cultivo crecía en condiciones no limitantes. El punto de partida fueron los coeficientes del grupo IV que por defecto se encuentran en los archivos especie, ecotipo y cultivar. Modificaciones menores fueron hechas para ajustar la fenología y dinámica del crecimiento para ambos cultivares. Con el archivo especie que aparece por defecto, el total de materia seca,  el aumento en el número de vainas y el crecimiento de las mismas fue subestimado. El archivo especie fue modificado para reducir la temperatura base cardinal para la fotosíntesis y formación de vainas. Con estas modificaciones se obtuvieron buenas predicciones para el crecimiento y rendimiento. También se evaluaron los cambios futuros en el rendimiento del cultivo de soja debido al cambio climático global a través del modelo CROPGRO-soybean calibrado, utilizando las proyecciones para nuestra región del modelo climático regional PRECIS bajo el escenario SRESA2 en los años 2030 y 2060. Para el cultivo creciendo en condiciones de secano, los aumentos previstos en el rendimiento son de 34 y 38 % para cada uno de los años estudiados, con una leve mejora atrasando la fecha de siembra.","palabras_clave":"Agrometeorología|modelo de cultivo|rendimiento|soja"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3862,"idioma_id":5,"titulo":"Espacio domestico, paisaje y relaciones sociales en el Valle de Balcosna, Prov. de Catamarca","resumen":"En este trabajo analizamos un espacio doméstico de la segunda mitad del primer milenio DC, ubicado en las serranías del valle de Balcosna, inserto en un paisaje con características comunales vinculado a actividades agro-pastoriles. Nuestro objetivo es aportar mayor información respecto a la zona de las estribaciones finales de las yungas, área ésta que fue siempre explicada en relación con las investigaciones e interpretaciones que se generaron en valles vecinos. Metodológicamente realizamos una revisión sobre la concepción arqueológica de esta zona, vinculamos nuestra perspectiva sobre la estructuración del paisaje, a través de clasificaciones y aplicamos los lineamientos de la arqueología de los espacios domésticos para delimitar diferentes contextos de uso de una estructura habitacional, que será interpretada en relación al paisaje re-creado socialmente. El uso del espacio y la apropiación territorial proporcionaron a nivel local las respuestas a las demandas comunitarias, asegurando entre otras cuestiones, la reproducción social.","palabras_clave":"doméstico|espacio|paisaje|Balcosna"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3863,"idioma_id":4,"titulo":"Assessing the impact of land use and land cover in water quality in the watershed of a reservoir","resumen":"Natural forest of Río Tercero watershed (Argentina) was replaced by urban expansion and agricultural activities causing negative impacts in water quality of Río Tercero reservoir. This paper classifies land use and land cover (LULC) in the watershed, trying to find a relationship with nutrient loads of the reservoir´s tributaries. Each tributary was analysed during 2006 bimonthly for physico-chemical variables. LULC was determined using a Landsat 5 TM image. Statistical analyses were carried out to identify relationships between water quality and LULC. Results suggest that urban and agricultural activities are the primary driving forces behind the variations in nutrient loads measured in tributaries. Sub-watershed most affected by human activities displayed the highest values of nutrient loads. If agricultural and urban activities continue to increase, water quality at watershed scale will decline. It is recommended the development of a global approach management plan at a watershed scale.","palabras_clave":"BASIN|LAND USE CHANGE |NUTRIENT LOADS|WATER QUALITY|WATER RESOURCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3864,"idioma_id":4,"titulo":"Acid-Doped ABPBI Membranes Prepared by Low-Temperature Casting: Proton Conductivity and Water Uptake Properties Compared with Other Polybenzimidazole-Based Membranes","resumen":"Phosphoric acid doped ABPBI (poly [2,5-benzimidazole]) membranes were prepared with a new low temperature casting procedure (ABPBI-ET), and their water uptake and proton conductivity were measured. The results were compared with those of ABPBIcasted at high temperatures (ABPBI-MSA), poly [2-2-(m-phenylene)-5-5 bibenzimidazole] (PBI), and commercial cross- linked Fumatech ABPBI (ABPBI-C) membranes. The water uptake of ultra thin ABPBI-ET membranes in the range 7?30 nm supportedover gold substrate, was also measured and compared with PBI ones. The in-plane proton conductivities of all the membranes were measured in the temperature range from 20&#9702; to 120&#9702;C at different water activities. The ABPBI-ET doped in 10.6 M H3PO4and ABPBI-C doped in 14.9 H3PO4 showed the highest conductivities, even higher than those reported for Nafion membranes. ABPBI-ET membranes showed a maximum as a function of water activity at aw &#8776; 0.55, a behavior that can be rationalized in termsof the dissociation constant of the ABPBI-H3PO4 complexes calculated using the Scatchard method, and the H3PO4 concentration in the water inside the membrane.","palabras_clave":"ABPBI|FUEL CELLS|MEMBRANES|PROTON CONDUCTIVITY|WATER UPTAKE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3865,"idioma_id":4,"titulo":"Fractional statistical theory of finite multilayer adsorption","resumen":"In the present paper, finite multilayer adsorption is described as a fractional statistics problem, based on Haldane´s statistics. In this scheme, the Helmholtz free energy and its derivatives are written in terms of a parameter g, which relates to the configuration of the molecules in the adsorbed state. For values of g ranging between 0 and 1 the formalism is used to model experimental data of bovine serum albumin (BSA) adsorbed onto an ion exchange resin for different values of pH and temperature. Excellent agreement between theory and experiments was found.","palabras_clave":"Adsorption isotherms|Multilayer adsorption|Equilibrium thermodynamics|statistical mechanics BSA protein adsorption"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3867,"idioma_id":4,"titulo":"Stress-induced Gene Expression Sensing Intracellular Heating Triggered by Magnetic Hyperthermia","resumen":"It is known that alternating magnetic field applications on eukaryotic cells loaded with single domain iron oxide nanoparticles result in high hyperthermic citotoxicity leading to cell dead. Although magnetic hyperthermia therapy for cancer tumours is being developed under this idea, some in vitro assays have shown controversial results indicating that alternating magnetic field triggers large apoptotic effect without significant culture-temperature increase. In agreement with these observations a huge lowering in nanoparticle specific heating rates, when going from the colloidal suspension to cell endosomes, together with cell death, has been reported. Here, we propose a new methodology to determine the occurrence of local heating in cells when alternating magnetic fields in the radiofrequency field range are applied to cell cultures holding very low iron oxide concentrations, being these concentrations insufficient to produce a global cell-culture temperature increase up to therapeutic values. To this end, human lung adenocarcinoma cells (A549 cell line) were transduced with a lentiviral vector encoding the expression of the enhanced green fluorescence protein, EGFP, under the action of the inducible human heat shock protein 70B promoter. This modified A549 cell line was incubated with aqueous suspensions of magnetite core nanoparticles (uncoated or covered with coating agents like citric acid or silicon oxide), and exposed to radiofrequency fields. The application of an alternating magnetic field to cell cultures loaded with nanoparticles resulted in no global temperature increase but EGFP expression. Stress-inducible gene expression scales with uptake and nanoparticle properties like saturation magnetization and heat dissipation efficiency. Our analysis demonstrates that EGFP expression is linked to a localized intracellular temperature increase.","palabras_clave":"gene expression|intracellular heating|cellular stress|magnetic hyperthermia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3868,"idioma_id":5,"titulo":"RELEVAMIENTO DE ESCARABEIDOS EN PASTIZAL NATURAL, PASTO LLORÓN Y MAIZ, EN EL  ESTE DE  SAN LUIS","resumen":"Los escarabeidos son importantes integrantes de la fauna edafícola pero pueden convertirse en plaga en suelos con siembra directa y en aquellos poco perturbados. Con el fin de determinar las especies de Scarabaeidae y su abundancia en tres condiciones, suelo cultivado (maíz), pasto llorón implantado y pastizal natural, en la Región Fitogeográfica I de la provincia de San Luis (Argentina), se realizaron muestreos completamente aleatorizados, durante dos períodos. En cada condición se tomaron tres muestras de suelo de 1\/8 de m2 a 30 cm de profundidad. Las larvas de escarabeidos se determinaron a nivel de especie según claves específicas, observándose ocho especies. Se determinaron en todas las condiciones de disturbio de suelo: Cyclocephala modesta, Cyclocephala putrida y Anomala testaceipennis, siendo las dos primeras las más abundantes; Lyogenis sp. y Philochloenia bonariensis se observaron sólo en pastizal natural, Cyclocephala signaticollis sólo en pasto llorón, Bothinus striatellus estuvo presente sólo en maíz y Leucothyreus sp. se presentó en pasto llorón y maíz.","palabras_clave":"ESCARABEIDOS|PASTIZAL NATURAL|CULTIVOS|SAN LUIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3869,"idioma_id":5,"titulo":"ARTRÓPODOS (PERJUDICIALES Y BENÉFICOS) Y MALEZAS EN EL PRIMER AÑO DE IMPLANTACIÓN DE ALFALFA, EN LA PROVINCIA SAN LUIS","resumen":"La alfalfa es la especie forrajera base de la producción de carne y de leche en la Región Pampeana. Su establecimiento, productividad y persistencia, dependen de numerosos factores, entre los cuales figuran los relacionados con la sanidad del cultivo en su primer año de implantación. Con la finalidad de conocer las especies perjudiciales (insectos y malezas) y especies benéficas (artrópodos) y sus  poblaciones, es que se  consideró importante el presente estudio. Los resultados obtenidos en cuanto a  diversidad, niveles poblacionales de artrópodos y cobertura de malezas, según las técnicas de muestreo recomendadas para cada caso, permiten conocer para la zona centro este de la provincia de San Luis, las especies que pueden comportarse como potenciales plagas y controladores bilógicos. Junto con prácticas de manejo constituyen herramientas del manejo integrado de problemas fitosanitarios del cultivo.","palabras_clave":"ALFALFA|MALEZAS|ARTRÓPODOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3870,"idioma_id":4,"titulo":"Lead pollution from waterfowl hunting in wetlands and rice fields in Argentina","resumen":"The pollution of wetlands by lead derived from waterfowl hunting with lead shot was nvestigated. We determined soil pellet density and Pb concentration in soil, water and vegetation in natural wetlands and rice fields in central-eastern Santa Fe province, Argentina. Pellet density varied greatly among hunting sites (between 5.5?141 pellets\/m2) and pellets were present in some control sites. Soil Pb concentration in most hunting sites (approximately 10?20 mgkg&#8722;1) was not much higher than in control sites (~5?10 mgkg&#8722;1), with the exception of the sitewith highest pellet density,which also had a high Pb soil concentration. Inwater, on the other hand, Pb concentration was similar in all sites (~4?7 ìg L&#8722;1), both control and hunting, and higher than reference values for aquatic media. Lead was also present in vegetation, including grasses and rice crops, in almost all cases. Most soil-collection sites were slightly acidic, and were frequently flooded. These results strongly suggest that metallic Pb from spent shot is oxidized and dissolved due to wetland conditions. Thus, the pollutant is readily mobilized and distributed across allwetland areas, effectively homogenizing its concentration in locations with and without hunting activities. The replacement of lead by nontoxicmaterials in pellets appears to be the only effective way to prevent Pb pollution in wetlands","palabras_clave":"LEAD|WETLANDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3871,"idioma_id":4,"titulo":"Metaheuristics approaches to solve combinatorial optimization problems in distribution power systems. An application to Phase Balancing in low voltage three-phase networks","resumen":"Metaheuristics algorithms are widely recognized as one of most practical approaches for combinatorial optimization problems. One the most interesting areas of application are the power systems. In particular, Distribution Systems planning and operation. This paper presents some metaheuristics approaches to solve a typical combinatorial optimization problem: the Phase Balancing in Low Voltage Electric Distribution Systems. A model supported in Linear Integer-Mixed Programming is presented, to observe and discussing its limitations. From this, is introduced a new metaheuristic, called Fuzzy Evolutionary Particle Swarm Optimization, based in the Swarm Intelligence Principles and Evolution Strategies, which is extended to fuzzy domain to modeling a multi-objective optimization, by mean of a fuzzy fitness function. A simulation on a real system is presented, and advantages of this approach respect to the Classical Simulated Annealing and Particle Swarm metaheuristics, selected between the most representatives, are evidenced.","palabras_clave":"Metaheuristic algorithm|Swarm Intelligence|Fuzzy sets|Electric distribution|Phase Balancing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3872,"idioma_id":4,"titulo":"Investigating the Uptake and Some Subcellular Effects of Manufactured Goethite Nanoparticles on Lumbriculus variegatus","resumen":"Objective: the aims of this work were: 1) to investigate whether the freshwater oligochaete Lumbriculus variegatus incorporates manufactured goethite nanoparticles (NPs) after an acute exposure; 2) to evaluate the influence of a solution of humic acids (HA) on the uptake of NPs; 3) to investigate the effects of these NPs on several biomarkers related to the generation of reactive oxygen species (ROS).Methods: NPs were analyzed by transmission electron microscopy. To evaluate the uptake, animals were exposed to 10 mg NPs L-1 for 48 h and then depurated for different periods (0; 4; 16, and 24 h). Iron concentrations were measured by atomic absorption spectrophotometry. Biomarker parameters were determined in oligochaetes exposed to a suspension containing 10 mg NPs L-1 for 48 h without depuration.Results: oligochaetes depurated for 4 h presented higher levels of Fe in their whole body tissues than control organisms. However, values returned to control levels when the oligochaetes were depurated for periods longer than 16 h, suggesting that organisms may transitorily incorporate the NPs in their digestive tract and\/or superficially adsorbed them but they could not absorb the NPs. Exposures for periods longer than 48 h did not increase the Fe body burden. The presence of a 20 mg L-1 of humic acid solution prevented the NPs uptake. Several biomarker parameters related to the generation of reactive oxygen species were assessed. The activity of the enzyme superoxide dismutase and levels of total glutathione were higher in animals exposed to the NPs for 48 h than in controls. However, the catalase activity and the determination of the total scavenger capacity (TOSC assay) were not modified. Conclusion: the release of manufactured nanomaterials to the aquatic environment may elicit some subcellular responses even when the NPs are not absorbed by the organisms.","palabras_clave":"GOETHITE|NANOPARTICLES|FRESHWATER INVERTEBRATES|LUMBRICULUS VARIEGATUS|UPTAKE|BIOMARKERS|HUMIC ACIDS|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3873,"idioma_id":5,"titulo":"Anencefalia asociada a trisomía 18 con elevada alfa-1-fetoproteína en líquido amniótico. Un caso reportado en San Luis. Argentina","resumen":"Se presenta un caso de anencefalia con trisomía 18, diagnosticado prenatalmente mediante screening ecográfico y cariotipo en cultivo de células amnióticas. Se encontraron niveles elevados de alfa-1-fetoproteína (AFP) en el líquido amniótico de una mujer de 33 años de edad, en la semana 15 de gestación. Como los fetos con trisomía 18 están sujetos a la pérdida temprana  o a un parto prematuro, las características físicas más sutiles de esta condición no suelen ser evidentes. Nuestro caso es compatible con la premisa de que la anencefalia es parte del fenotipo de la trisomía 18. Por lo tanto, la realización del cariotipo en los fetos con anencefalia debe ser considerado.","palabras_clave":"Anencefalia|Alpha-1 fetoproteina|Trisomia 18"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3874,"idioma_id":4,"titulo":"'Development of nitrocellulose membrane filters impregnated with different biosynthesized silver nanoparticles applied to water purification'","resumen":"'Development of nitrocellulose membrane filters impregnated with different biosynthesized silver nanoparticles applied to water purification'","palabras_clave":"nitrocellulose membrane filters|biosynthesized silver nanoparticles|water purification|Yeasts"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3875,"idioma_id":4,"titulo":"An electrochemical immunosensor for anti-T. cruzi IgM antibodies, a biomarker for congenital Chagas disease, using a screen-printed electrode modified with gold nanoparticles and functionalized with shed acute phase antigen","resumen":"The authors describe a sandwich immunosensor for anti-IgM T. cruzi-specific antibodies which are a biomarker for congenital Chagas disease (CCD). A screen-printed carbon electrode (SPCE) was modified with gold nanoparticles (AuNPs) and then functionalized with recombinant shed acute phase antigen (SAPA). The AuNPs\/SPCE was characterized by scanning electron microscopy, cyclic voltammetry, and X-ray diffraction. The AuNPs provide an increased active surface area, high conductivity, and improved electrocatalytic characteristics, this resulting in a significant improvement of the detection limit. Following interaction between immobilized SAPA and the target antibodies in the serum sample, secondary antibody (an anti-human IgM-HRP conjugate) and solutions of H2O2 and 4-tert-butylcatechol were added and the enzymatic product was detected at &#8722;100 mV (vs. Ag\/SPCE). The resulting current was proportional to the anti-T. cruzi IgM antibodies present in the sample and showed a linear trend from 10 to 200 ng mL&#8722;1 (R = 0.998). The detection limit was 3.03 ng mL&#8722;1. These values demonstrate that this electrochemical immunosensor can be used as an alternative serological method for diagnosis of congenital Chagas disease.","palabras_clave":"Sandwich immunoassay|Recombinant antigen|Scanning electron microscopy|Cyclic voltammetry|X-ray diffraction|Horseradish peroxidase|Serology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3876,"idioma_id":4,"titulo":"The Chloride Anion Acts as a Second Messenger in Mammalian Cells - Modifying the Expression of Specific Genes","resumen":"Background\/Aims: Cystic Fibrosis (CF) is caused by mutations in the gene CFTR, encoding a cAMP-activated chloride (Cl-) channel. We have previously demonstrated that the expression of several genes can be modulated by the CFTR activity; among them, SRC, MTND4, CISD1, and IL1B. However, the CFTR signalling mechanism involved in the expression of CFTR-dependent genes is unknown. The aim of this work was to determine if intracellular chloride (Cl-)i might function as a second messenger modulating the expression of specific genes. Methods: Differential display (DD) was applied to  IB3-1 cells (CF cells), cultured under conditions that produce different intracellular Cl- concentrations ([Cl-]i), to analyse their expression profile. Results: Several differentially expressed gene products were observed by using DD, suggesting the presence of chloride-dependent gene expression. Two cDNA fragments, derived from differentially expressed mRNAs and showing opposed response to Cl-, were isolated, cloned, sequenced and its Cl- dependency validated by reverse transcription quantitative-PCR (RT-qPCR). We identified the gene RPS27, which encodes the multifunctional ribosomal protein RPS27, also known as metallopanstimulin-1 (MPS-1), and the gene GLRX5, encoding glutaredoxin-related protein 5, as chloride-dependent genes. RPS27 was negatively regulated with increased [Cl-]i, approximately from 25-75 mM Cl- (EC50 = 46 ± 7 mM), and positively regulated  from 75-125  mM Cl- (EC50 = 110 ± 11 mM) (biphasic response). In contrast, GLRX5 was positively modulated by [Cl-]i, showing a typical sigmoidal dose-response curve from 0-50 mM Cl-, reaching a plateau after 50 mM Cl- (EC50 ~ 34 mM). Conclusion: The results suggest the existence of chloride-dependent genes. The Cl- anion, therefore, might act as a second messenger for channels or receptors able to modulate the intracellular Cl- concentration, regulating in turn the expression of specific genes.","palabras_clave":"CFTR|CLORURO|SEGUNDO MENSAJERO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3877,"idioma_id":4,"titulo":"Improving the antidepressant action and the bioavailability of sertraline by co-crystallization with coumarin 3-carboxylate. Structural determination","resumen":"The salt generated with protonated sertraline and coumarin 3-carboxylate, for shortSerH-CCA, was synthesized and characterized by means of FTIR spectroscopy. Thecrystal structure was determined by X-ray diffraction methods. The substance cocrystallizesin the monoclinic P21 space group with a=11.4045(6) A, b=8.1812(5) A,c=12.7394(5) A, â=91.792(4)°, and Z=2 molecules per unit cell. Thepharmacotherapeutic agent sertraline (Ser) and the antioxidant compound coumarin 3-carboxylic acid (CCA) appear in the solid as their protonated cationic (SerH+) anddeprotonated anionic (CCA-) moieties. The crystal salt is further stabilized by strongintermolecular (Ser) &gt;NH2+N-OOC-(CCA) bonds. The antidepressant effect of the newcompound has shown a higher power than sertraline hydrochloride alone when testedon male Wistar rats.P21 space group with a=11.4045(6) A, b=8.1812(5) A,c=12.7394(5) A, â=91.792(4)°, and Z=2 molecules per unit cell. Thepharmacotherapeutic agent sertraline (Ser) and the antioxidant compound coumarin 3-carboxylic acid (CCA) appear in the solid as their protonated cationic (SerH+) anddeprotonated anionic (CCA-) moieties. The crystal salt is further stabilized by strongintermolecular (Ser) &gt;NH2+N-OOC-(CCA) bonds. The antidepressant effect of the newcompound has shown a higher power than sertraline hydrochloride alone when testedon male Wistar rats.2+N-OOC-(CCA) bonds. The antidepressant effect of the newcompound has shown a higher power than sertraline hydrochloride alone when testedon male Wistar rats.N-OOC-(CCA) bonds. The antidepressant effect of the newcompound has shown a higher power than sertraline hydrochloride alone when testedon male Wistar rats.","palabras_clave":"SERTRALINE COUMARIN 3-CARBOXYLATE.|COUMARIN 3-CARBOXYLIC ACID. |COCRYSTALLIZATION|ANTIDEPRESSANT EFFECT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3882,"idioma_id":4,"titulo":"Ciliate protozoa of the forestomach of llamas (Lama glama) from locations at different altitude in Argentina","resumen":"This study describes the diversity and concentration of the protozoal population from the forestomach of llamas in Argentina at three altitudinal locations. Protozoal diversity was studied in samples from eight llamas from Hurlingham (Buenos Aires, 43 m altitude), four from Tilcara (Jujuy, 2465 m altitude) and six llamas from Cieneguillas (Jujuy, 3800 m altitude). The total concentrations of protozoa in the forestomach contents were 7.9, 9.1 and 4.1 cells x 104 ml-1 in Hurlingham, Tilcara and Cieneguillas, respectively (P>0.05). Entodinium spp. represented 97.9, 92.3 and 71.4% of the protozoal community in Hurlingham, Tilcara and Cieneguillas, respectively, and the remaining protozoa belonged to the Eudiplodinium genus. Entodinium spp. were identified as E. caudatum (mostly morphotype dubardi), E. longinucleatum, E. parvum, E. bovis, E. exiguum, E. dubardi, and a minor presence of E. bimastus (in three animals) and E. ovibos (in one animal). In regards to the rest of protozoal species, Eudiplodinium maggii is the first reported host record for the genus in llamas. This species was present in the forestomach of 14 out of 18 llamas tested, and in one case it was the unique protozoal species. The vestibuliferids, Dasytricha and Isotricha were absent from the forestomach of llamas. Similarly, other species such as those from the Caloscolex genus, Diplodinium cameli and Entodinium ovumrajae, commonly found in Old World Camelids, were also absent from llamas.","palabras_clave":"FORESTOMACH PROTOZOA|SOUTH AMERICAN CAMELIDS|ENTODINIOMORPHIDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3883,"idioma_id":4,"titulo":"The Celliforma ichnofacies in calcareous paleosols: an example from the late Miocene Cerro Azul Formation, La Pampa, Argentina","resumen":"Invertebrate trace fossils from the late Miocene Cerro Azul Formation (La Pampa, Argentina) are described and compared with known paleosol ichnofacies. The formation is a loess succession punctuated by numerous calcareous paleosols. Trace fossils were recovered from thirteen localities distributed in a wide latitudinal and longitudinal range within La Pampa Province. Paleosols from the northwestern localities display clastic dikes and pedogenic slickensides and are compared with calcic vertisols, which are typical of strongly seasonal and semiarid climate. Paleosols from the remaining localities have a Bt horizon with blocky or prismatic peds and can be compared with mollisols. The depth to the Bk horizon for all paleosols averages 0.54 m, suggesting a mean annual precipitation of 450 mm (± 147 mm), also pointing to semiarid climatic conditions during soil formation. The trace fossil assemblage from the Cerro Azul Formation is of low diversity and abundance and is typified by the presence of the insect trace fossils Celliforma, Rosellichnus, Rebuffoichnus, Fictovichnus and Teisseirei. The ichnospecies recorded are: Celliforma germanica, Celliforma cf. rosellii, Celliforma isp., Rosellichnus isp., Rebuffoichnus casamiquelai, Fictovichnus cf. sciuttoi, Fictovichnus isp., Teisseirei barattinia, Taenidium barretti, and cylindrical burrow fills. These trace fossils (along with Attaichnus kuenzelii, vertebrate burrows and medium?sized rhizoliths described in previous works) are considered comparable with the Celliforma ichnofacies. This ichnofacies is indicative of well-drained calcareous paleosols developed under low vegetation coverage. For the analyzed succession the vegetation was probably dominated by scrubs with minor participation of herbaceous plants. Coprinisphaera ispp. (including C. lazai, C. murguiai, and Coprinisphaera isp.) showing a cementation similar to associated late Miocene trace fossils were only recorded, along with Celliforma isp., in two localities. This trace fossil assemblage is comparable with the Coprinisphaera ichnofacies, suggesting dominance of herbaceous communities and wetter climate than those of the Celliforma ichnofacies. Some specimens of uncemented Coprinisphaera ispp. were produced during post-Miocene times and are not considered as a part of the trace fossil assemblage of the Cerro Azul Formation.","palabras_clave":"Paleosol ichnofacies  |Celliforma|Rebuffoichnus |Fictovichnus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3884,"idioma_id":4,"titulo":"Involvement of protein cAMP-dependent kinase, phospholipase A2 and phospholipase C in sperm acrosome reaction of Chinchilla lanigera","resumen":"The mechanisms involved in fertilization are the centre of attention in order to determine the conditions required to reproduce in vitro the events that take place in vivo, with special interest in endangered species. Previous data from mouse sperm, where acrosome reaction (AR) occurs more often in the interstitium of the cumulus oophorus, contribute to strengthen the use of progesterone as a physiological inducer of this process. We studied the participation of protein kinase A (PKA), phospholipases A2 and C (PLA2 , PLC) in the AR induced by progesterone from Chinchilla epididymal spermatozoa. The addition of db-cAMP to the incubation medium caused an increase of 58% in the AR, while the use of H89 (30 &#956;m), a PKA inhibitor, reflected a decrease of 40% in the percentage of reacted gametes. The assays conducted with arachidonic acid showed a maximum increase of 23% in the AR. When gametes were pre-incubated with PLA2 inhibitors, a dose-dependent inhibitory effect was observed. The addition of phorbol12-myristate13-acetate (10 &#956;m) revealed higher percentages of AR induction (60%). When PLC was inhibited with neomycin and U73122, a dose-dependent decrease in AR percentages was observed. Combined inhibition of PKA, PLA2 and PLC, AR values similar to control were obtained. This work shows evidence, for the first time in Chinchilla, that progesterone activates the AC\/cAMP\/PKA system as well as sperm phospholipases and that these signalling pathways participate jointly and cooperatively in AR. These results contribute to the understanding of the complex regulation that is triggered in sperm after the effect of progesterone.","palabras_clave":"PHOSPHOLIPASE A2|SPERM|PHOSPHOLIPASE C"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3885,"idioma_id":4,"titulo":"Spread of Phlebotominae in temperate climates: province of Córdoba, Argentina","resumen":"We report the presence of the competent vector for Leishmania spp, Migonemyia migonei, and the Evandromyiacortelezzii-sallesi complex south of its known distribution in the central temperate region of Argentina, in theprovince of Córdoba. The persistence of this phlebotomine in the northern border of the province, its associationwith a case of cutaneous leishmaniasis, and the new record in the outskirts of the city of Córdoba, the second mostpopulated in the country, strengthens the need for regular vector surveillance and a case detection-sensitive healthsystem in vulnerable regions, even in temperate climates.","palabras_clave":"PHLEBOTOMINAE|DISTRIBUCIÓN|CORODBA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3886,"idioma_id":4,"titulo":"Culicidae (Diptera) community structure, spatial and temporal distribution in three environments of the province of Chaco, Argentina","resumen":"The aim of this work was to study the composition of the Culicidae community in three environments with  different degrees of anthropic disturbance in the province of Chaco. The nonparametric richness estimator ACE was calculated to measure the completeness of the sampling in each environment, and the diversity of each environment (-diversity) were estimated. The composition, abundance and uniformity of species from the different environments were compared by means of range-species curves. -diversity  was measured as species complementarity, which allowed us to know the degree of dissimilarity among the environments. The synanthropic index was estimated, identifying urban environment (synanthropic) species, eusynanthropic species, and wild species. The in&#64258;uence of climatic factors (mean temperature,  relative humidity and rainfall) on the monthly and annual variations of the identi&#64257;ed mosquito species was analysed using multilevel Poisson models with over-dispersion. The wild environment showed higher diversity and the semi-urban environment higher species richness. Despite having lower S and abundance, the urban environment showed greater diversity than semi-urban environment, although it also showed lower completeness, which means S could increase. Anthropogenic disturbance can lead to the elimination and\/or modi&#64257;cation or substitution of habitats, with the subsequent loss ofrichness in the more sensitive species of Culicidae fauna, although the conditions are also favourable for the settling of opportunistic and exotic species well-adjusted to disturbed environments. Most of the species abundances were positively in&#64258;uenced by the analysed climatic variables. The study area showed a rich Culicidae fauna of public health signi&#64257;cance, with a risk of pathogen transmission, suggesting the need of further studies and the febrile and entomological surveillance.","palabras_clave":"CULICIDAE|DISTRIBUCION|ESPACIAL|TEMPORAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3887,"idioma_id":4,"titulo":"New Precipitation and Temperature Grids for Northern Patagonia: Advances in Relation to Global Climate Grids","resumen":"Climate data compiled from different sources in Northern Patagonia were interpolated to 20 km resolution grids of mean monthly temperature and total monthly precipitation over the period 1997-2010. This Northern Patagonia Climate Grid (NPCG) improves on previous gridded products in its spatial resolution and the number of contributing stations, since it incorporates 218 and 114 precipitation and temperature records, respectively. A geostatistical method using surface elevation from a Digital Elevation Model as ancillary variable was used to interpolate station data into even spaced points. The maps provided by NPCG are consistent with the broad spatial and temporal patterns of northern Patagonia climate, showing a comprehensive representation of the latitudinal and altitudinal gradients in temperature and precipitation, as well their related patterns of seasonality and continentality. We compared the performance of NPCG and other different data sets available to the climate community for northern Patagonia. The grids used for the comparison included the Global Precipitation Climatology Project (GPCP), the ERA-I reanalysis, the Climate Research Unit (CRU) from University of East Anglia and the University of Delaware (UDEL). NPCG outperforms other global grids based on BIAS, MAE and RMSE, three statistics that quantitatively assess the spatial coherence of gridded data against available observations. NPCG represents a useful tool for understanding climate variability in northern Patagonia and a valuable input for regional models of hydrological and ecological processes. Its resolution is optimal to validate data from the General Circulation Models (GCM) and for working with raster data derived from remote sensors such as vegetation indices.","palabras_clave":"precipitación |temperatura|Co-Kriging|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3888,"idioma_id":4,"titulo":"Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana","resumen":"Plant-based platforms are extensively used for the expression of recombinant proteins, including monoclonal antibodies. However, to harness the approach effectively and leverage it to its full potential a better understanding of intracellular processes that affect protein properties is required. In this work we examined vacuolar targeting and deposition of the monoclonal antibody (Ab) 14D9 in Nicotiana benthamiana leaves. Two distinct vacuolar targeting signals (KISIA and NIFRGF) were C-terminal fused to the heavy chain of 14D9 (vac-Abs) and compared with secreted and ER retained variants (sec-Ab, ER-Ab, respectively). Accumulation of ER- and vac-Abs were 10-15-fold higher than sec-Ab. N-glycan profiling revealed the predominant presence of plant typical complex fucosylated and xylosylated GnGnXF structures on sec-Ab while vac-Abs carried mainly oligomannosidic (Man 7-9) next to GnGnXF forms. Paucimannosidic glycans (commonly assigned as typical vacuolar) were not detected. Confocal microscopy analysis using RFP fusions showed that sec-Ab-RFP localized in the apoplast while vac-Abs-RFP were exclusively detected in the central vacuole. The data suggest that vac-Abs reached the vacuole by two different pathways: direct transport from the ER bypassing the Golgi (Ab molecules containing Man structures) and trafficking through the Golgi (for Ab molecules containing complex N-glycans). Importantly, vac-Abs were correctly assembled and functionally active. Collectively, we show that the central vacuole is an appropriate compartment for the efficient production of Abs with appropriate posttranslational modifications, but also point to a reconsideration of current concepts in plant glycan processing.","palabras_clave":"Vacuolar targeting|recombinant antibodies|Plant body|Molecular farming"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3889,"idioma_id":4,"titulo":"Biochemical, biophysical, and functional properties of ICA512\/IA-2 RESP18 homology domain","resumen":"Background: ICA512 (or IA-2\/PTPRN) is a transmembrane protein-tyrosine phosphatase located in secretorygranules of neuroendocrine cells. Previous studies implied its involvement in generation, cargo storage, traffic,exocytosis and recycling of insulin secretory granules, as well as in &#946;-cell proliferation. While several ICA512domains have been characterized, the function and structure of a large portion of its N-terminal extracellular(or lumenal) region are unknown. Here, we report a biophysical, biochemical, and functional characterizationof ICA512?RESP18HD, a domain comprising residues 35 to 131 and homologous to regulated endocrinespecificprotein 18 (RESP18).Methods: Pure recombinant ICA512?RESP18HD was characterized by CD and fluorescence. Its binding to insulinand proinsulin was characterized by ELISA, surface plasmon resonance, and fluorescence anisotropy. Thiol reactivitywas measured kinetically. Targeting of &#916;RESP18HD ICA512?GFP to the membrane of insulinoma cells wasmonitored by immunofluorescence.Results: ICA512?RESP18HDpossesses a strong tendency to aggregate and polymerize via intermolecular disulfideformation, particularly at pH N 4.5. Its cysteine residues are highly susceptible to oxidation forming an intramoleculardisulfide between cysteine 53 and 62 and intermolecular disulfides via cysteine 40 and cysteine 47. Theregulated sorting of ICA512 to secretory granules in INS-1 cells was impaired by deletion of RESP18HD.ICA512?RESP18HD binds with high-affinity to insulin and proinsulin.Conclusions: RESP18HD is required for efficient sorting of ICA512 to secretory granules.General significance: RESP18HD is a key determinant for ICA512 granule targeting.","palabras_clave":"ICA512|IA-2|RESP18|Diabetes|Insulin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3891,"idioma_id":4,"titulo":"Mild Prolonged Chronic Hyponatremia and Risk of Hip Fracture in the Elderly","resumen":"ABSTRACTBackground: Hip fractures are among the most serious bone fractures in the elderly, producing significant morbidity and mortality. Hyponatremia can adversely affect bone, with fractures occurring as a potential complication. We examined if there is an independent association between prolonged chronic hyponatremia (> 90 days duration) and risk of hip fracture in the elderly. Methods: Retrospective cohort study in adults aged over 60 years from a prepaid health maintenance organization who 2 or more measurements of plasma sodium between 2005 and 2012. Hip fractures incidence was assessed in subjects with prolonged chronic hyponatremia defined as plasma sodium values <135 mmol\/L lasting more than 90 days. Multivariable Cox regression was performed to determine hazard ratio for hip fracture risk associated with prolonged chronic hyponatremia after adjustment for the propensity to have hyponatremia, fracture risk factors and relevant baseline characteristics. Results: Among 31,527 eligible patients, 228 (0.9%) had prolonged chronic hyponatremia. Mean plasma sodium was 132±5 mmol\/L in hyponatremic patients, and 139±3 mmol\/L in normonatremic patients (P<0.001). Hip fractures incidence was 781\/100,000 person-years (95% CI: 463?1320) in hyponatremic patients, and 365\/100,000 person-years (95% CI: 333?400) in normonatremic patients. Hyponatremic patients had a substantially elevated rate of hip fracture (adjusted hazard ratio 4.52, 95% CI: 2.14?9.6), which was even higher in those with moderate hyponatremia (< 130 mmol\/l) (adjusted hazard ratio 7.61, 95% CI: 2.8 ? 20.5). Conclusion: Mild prolonged chronic hyponatremia is an 3independent risk factor for hip fracture in the elderly. Clinical trials to investigate whether correction of chronic hyponatremia reduces hip fracture risk are needed.","palabras_clave":"falls|hip fracture|hyponatremia|osteoporosis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3892,"idioma_id":4,"titulo":"Development of Ni-Rh-Al Catalysts for Methane Dry Reforming. Influence of the Method of Preparation","resumen":"The influence of the preparation method of the nickel-rhodium-aluminum based catalyst on its microstructure and catalytic activity for methane carbon dioxide reforming was studied. Aluminum sec-butoxide, Ni 1-metoxi 2-propoxide and rhodium nitrate were used as starting reactants. Three catalysts were prepared by the main methods: i) hydrolysis and thermal decomposition (TD), ii)  hydrolysis and acid peptization (sol-gel, SG) and iii) hydrolysis,thermal decomposition and hydrothermal treatment (TD-HT). The three catalysts were evaluated for a period of 30 hours with the same metallic composition, 14% Ni, 1.4% Rh and the same operating conditions namely temperature, 790 ºC; W\/FºCH4,in (relationship between mass of catalyst and the flow of methane in the feed stream), 0.5 g.h.mol-1 and CO2:CH4 ratio 1.0. The best bulk catalystidentified was (Ni-Rh-Al)TD-HT, showing excellent activity, selectivity, and stability. On the other hand the catalyst (Ni-Rh-Al)TD-HT was analyzed in a fixed bed reactor, modifying initial conditions such as temperature, W\/FºCH4,in and CO2:CH4 ratio for a period of 120 hours achieving excellent catalytic activity, selectivity and stability. Studies on all catalysts evaluated reveal a low filamentous-type carbon deposition.","palabras_clave":"CO2 REFORMING OF METHANE|NI- RH-AL2O3 CATALYSTS|SYNTHESIS GAS, HYDROGEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3893,"idioma_id":5,"titulo":"Autoridades escolares y TIC: Articulaciones y tensiones. Formación de directivos e inspectores en la UNIPE","resumen":"Este artículo pone a consideración el seminario «Las tic, la gestión educativa y la apropiación del espacio virtual»,desarrollado en el marco de la Diplomatura en Formación para Directores e Inspectores, que en forma conjunta coordinan la Universidad Pedagógica de la Provincia de Buenos Aires (unipe), la Organización de Estados Iberoamericanos (oei) y el Ministerio de Educación de la República Argentina, a través del programa Nuestra Escuela.Se presenta en forma breve la estructura curricular general de la diplomatura para contextualizar el seminario, y se aborda con mayor profundidad la organización de los contenidos, los encuentros presenciales y la producción de documentos educativos originados para el mismo, así como las actividades y encuentros virtuales planteados. Se exponen también las propuestas de trabajo obligatorias y optativas que se consideraron con la finalidad de interpelar a directores e inspectores acerca del rol que históricamente tuvieron, y cuánto y cómo lo ha modificado la llegada de las tecnologías de la información y la comunicación (tic). La lectura de los trabajos finales para la promoción es otra fuente documental que aporta al debate iniciado y abre nuevas líneas de trabajo para futuros seminarios y publicaciones.","palabras_clave":"entornos virtuales|gestión institucional|programas 1 a 1|TIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3894,"idioma_id":4,"titulo":"Small strongyles (cyathostomes) and benzimidazoles. Persistance of status of resistance after nine years without the use of these drugs and efficacy of ivermectin about this parasite population","resumen":"Background: in June 2005 was documented for the first time inArgentina (Santa Fe province) the presence in a horse farm ofcyathostome populations resistant to benzimidazole [1] .Fromthen on, the use of this chemical group was supplanted by ivermectinat this facility. The horse herd was relatively stable andclosed and a new study was conducted in June 2014 to determinewhether the absence of use of benzimidazoles may have affectedthe susceptibility of nematodes to this drug and compare theefficacy of ivermectin.","palabras_clave":"CYATHOSTOMES|BENZIMIDAZOLES|EPG|FECAL EGGS COUNT REDUCION TEST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3895,"idioma_id":4,"titulo":"Peripheral blood monocyte and T cell subsets in children with specific polysaccharide antibody deficiency (SPAD)","resumen":"Specific polysaccharide antibody deficiency (SPAD) is a well reported immunodeficiency characterized bya failure to produce antibodies against polyvalent polysaccharide antigens, expressed by encapsulatedmicroorganisms. The clinical presentation of these patients involves recurrent bacterial infections, beingthe most frequent agent Streptococcus (S.) pneumoniae. In SPAD patients few reports refer to cells otherthan B cells. Since the immune response to S. pneumoniae and other encapsulated bacteria was historicallyconsidered restricted to B cells, the antibody deficiency seemed enough to justify the repetitiveinfections in SPAD patients. Our purpose is to determine if the B cell defects reported in SPAD patientsare accompanied by defects in other leukocyte subpopulations necessary for the development of a properadaptive immune response against S. pneumoniae. We here report that age related changes observed inhealthy children involving increased percentages of classical monocytes (CD14++ CD16 cells) anddecreased intermediate monocytes (CD14++ CD16+ cells), are absent in SPAD patients. Alterations canalso be observed in T cells, supporting that the immune deficiency in SPAD patients is more complex thanwhat has been described up to now.","palabras_clave":"Primary immunodeficiency  | Pneumococcus Monocytes|T cells|Cell activation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3896,"idioma_id":4,"titulo":"Evaluation of Sorptive Capacity of Natural Clay, Pillared Clay, and Alga-modified Clay to Contain Oil Spills in Soil","resumen":"This paper describes the characterization of natural Patagonian clay (ARC), composed mainly ofmontmorillonite and illite, and two modifications: one with alga Codium fragile (ARC-ALG) and other ironpillared(ARC-PIL), as oil sorbents. The textural characterization of samples was performed by nitrogenadsorption isotherms at 77K, obtaining 36, 2 and 105 m2\/g of BET surface area, respectively. The pore sizedistribution was calculated from isotherms data by the method of density functional (NLDFT). Oilsorption capacities were determined and expressed in g oil\/g sorbent by tests in batch, sorption isothermswere obtained in a concentration range of 50 to 300 mg\/L of oil in organic solvent. The quantification ofremnant concentrations in solution was performed by UV-Vis spectrophotometric techniques. The results ofsorption capacity are consistent with the BET surface area for ARC and ARC-PIL, but are not in agreementwith ARC-ALG, since it has the lowest BET surface area and the highest sorption capacity; this would bemainly due to the organic nature of this material. This information together with the analysis of the respectiveisotherms, gives the possibility of making inferences about of the action mechanisms for the selection of newsorbents in treating soils spills.","palabras_clave":"OIL|CLAY|ALGAE|PILLARED|SOILS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3898,"idioma_id":4,"titulo":"Production of bacterial nanocellulose from wine industry residues: importance of fermentation time on pellicles characteristics","resumen":"Bacterial nanocellulose (BNC) was produced by Gluconacetobacter xylinus under static conditions using grape pomaceextract (the most abundant residue of the wine industry) as a carbon source and corn steep liquor (a byproduct of corn wet-milling)as the main nitrogen source. Carbon and nitrogen source concentrations, as well as inocula size, fermentation time, and temperature,were all considered in order to maximize BNC production by the use of statistically designed experiments and the response surfacemethodology. At optimum production conditions, the effect of fermentation time on morphology, solids content, chemical structure,crystallinity, thermal decomposition pattern, and storage modulus of dried BNC pellicles was analyzed. The results evidenced thatdried BNC pellicles that were incubated for longer times showed higher thermal stability, higher crystallinity, and higher storage modulus,resulting from a denser nanoribbon network. All of these characteristics will certainly play a role in the performance of BNC inpractical applications.","palabras_clave":"BIOPOLYMERS AND RENEWABLE POLYMERS|BIOSYNTHESIS OF POLYMERS|CELLULOSE AND OTHER WOOD PRODUCTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3899,"idioma_id":4,"titulo":"Magnetic mapping of air pollution in Tandil city (Argentina) using the lichen Parmotrema pilosum as biomonitor","resumen":"The lichen Parmotrema pilosum is sensitive to pollution and it can live accumulating airborne pollutantsfor long time, such characteristic allows its use as biomonitor for environmental mapping in urban areaswhen this epiphytic specie is available. In this work, we investigated the use of such passive collector andmagnetic techniques to monitor the air pollution in Tandil, a city located in Buenos Aires province withapproximately 125,000 inhabitants, 60,000 vehicles and various metallurgical factories inside the urbanarea. The sampling strategy was carried out following a random stratified design and measuring magneticsusceptibility, magnetic hysteresis loops, anhysteretic and isothermal remanent magnetization andthermomagnetic studies to determine the magnetic properties of airborne particles accumulated onlichen samples. Scanning electron microscopy observations show particles with different morphologies(individual particles, spherules and aggregates) and composition (Fe, Al, Ni, Cr, Ti, Cu, K and Br) producedby metallurgical factories and by gaseous\/solid vehicle emissions. The magnetic mineralogy shows thepredominance of pseudo-single domain magnetite-like mineral and the magnetic grain size estimationsindicate the presence of fine particles (<0.1 mm) in sites with low vehicular traffic or less polluted, whilesites more affected by pollution (high vehicular traffic and metallurgical industries) are characterized bycoarser magnetic grain size particles, between 0.1 and 5 mm. Mass-specific magnetic susceptibility wasrepresented in a 2-D contour map to observe in detail the distribution of magnetic particles in this urbanarea, giving high values (up to 1161.2  108 m3 kg1) that are indicative of areas with high pollutionloading.","palabras_clave":"BIOMONOTORING |MAGNETICS PROPERTIES|GRAIN SIZE|AIR POLLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3900,"idioma_id":5,"titulo":"*¿INSPIRAR SERES VIVOS?  UNA PROPUESTA DIDÁCTICA INTEGRADORA PARA INTRODUCIR A LOS ESTUDIANTES EN LA INVESTIGACIÓN EN BIOLOGÍA A TRAVÉS DE LA AEROBIOLOGÍA","resumen":"En el presente trabajo se expone una propuesta pedagógica para los niveles medio, preuniversitario y universitario inicial, en la cual, a través de un tema integrador como es la Aerobiología, los alumnos puedan arribar a conclusiones significativas en su proceso de aprendizaje de las ciencias. La idea de que se desarrolle esta temática se justifica en que la misma tiene relación con una gran diversidad de contenidos referidos a diversas disciplinas. La utilización del método científico como línea de pensamiento en este trabajo es de central atención  puesto que es ésta la metodología que los educandos van a utilizar para problematizar la temática, realizar predicciones acerca de la misma y elaborar su propio diseño experimental de laboratorio. El mismo constará de un muestreo ambiental en el que se valorará la calidad y cantidad de partículas aerobiológicas circundantes en el aire del lugar muestreado como pueden ser: granos de polen, esporas, principalmente, fúngicas, ácaros, etc. Los estudiantes serán provistos con los elementos necesarios para llevar a cabo la investigación. Ésta constará en colocar dispositivos de muestreo, en lugares de su elección, conformados por portaobjetos a los cuales se les pega una cinta Melinex impregnada con fluido de silicona transparente a donde se van a adherir las partículas biológicas. Luego, a la cinta se le coloca un cubreobjetos impregnado con fucsina básica que colorea solamente granos de polen depositados en la muestra, las otras estructuras no necesitan ser coloreadas. A continuación, los educandos llevarán las muestras al laboratorio para realizar una lectura de las mismas al microscopio óptico registrando los resultados en tablas y gráficos. Finalmente se realiza una exposición, con puesta en común, entre los diferentes grupos sacando conclusiones acerca de los resultados obtenidos y la viabilidad de cada diseño experimental propuesto. Los alumnos podrán verificar si sus predicciones con respecto a los resultados fueron  o no acertadas.","palabras_clave":"Aerobiología|Polen|Espora|Coevaluación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3902,"idioma_id":5,"titulo":"Modelo de predisposición adquirida para el uso de alcohol en adolescentes argentinos","resumen":"Se analizó, en una muestra de adolescentes argentinos, el modelo de predisposición adquirida. El modelo propone que el rasgo desinhibición influye de manera indirecta, a través de variables cognitivas, sobre el consumo de alcohol. La exposición a modelos de consumo del grupo de pares influye directa e indirectamente (mediante las expectativas hacia el alcohol) sobre el uso de alcohol. Participaron 343 adolescentes  asistentes a colegios públicos de educación media de la ciudad de Córdoba (Argentina). Se midió impulsividad, expectativas hacia el alcohol, motivos de consumo de alcohol, normas sociales de consumo y consumo de alcohol de los adolescentes. Para determinar el efecto de los variables señaladas como antecedentes del consumo se aplicó un análisis de senderos. Se propusieron dos modelos teóricos que diferían en la inclusión, o no, de las normas sociales de consumo de alcohol. Los resultados apoyan el modelo de predisposición adquirida, indicando que el efecto de impulsividad sobre el consumo de alcohol es indirecto mediado por las expectativas hacia el alcohol. Aunque ambos modelos presentan adecuado ajuste a los datos, el modelo que incorpora el efecto de las normas sociales de consumo presenta un ajuste excelente. Los resultados de este trabajo, el primero de nuestro medio en evaluar el modelo de predisposición adquirida sobre el consumo de alcohol, destacan la utilidad de diagramar esfuerzos preventivos focalizados en el control de los impulsos, las expectativas positivas hacia el alcohol y en la reducción de la percepción del consumo de los pares.","palabras_clave":"consumo de alcohol|adolescentes |modelo predisposición adquirida|impulsividad|expectativas hacia el alcohol|normas sociales."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3903,"idioma_id":4,"titulo":"Reactive oxygen species production by human dendritic cells involves TLR2 and dectin-1 and is essential for efficient immune response against Mycobacteria","resumen":"Tuberculosis remains the single largest infectious disease with 10 million new cases and two million deaths that are estimated to occur yearly, more than any time in history. The intracellular replication of Mycobacterium tuberculosis (Mtb) and its spread from the lungs to other sites occur before the development of adaptive immune responses. Dendritic cells (DC) are professional antigen-presenting cells whose maturation is critical for the onset of the protective immune response against tuberculosis disease and may vary depending on the nature of the cell wall of Mtb strain. Here, we describe the role of the endogenous production of reactive oxygen species (ROS) on DC maturation and expansion of Mtb-specific lymphocytes. Here, we show that Mtb induces DC maturation through TLR2\/dectin-1 by generating of ROS and through Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin (DC-SIGN) in a ROS independently manner. Based on the differences observed in the ability to induce DC maturation, ROS production and lymphocyte proliferation by those Mtb families widespread in South America, i.e., Haarlem and Latin American Mediterranean and the reference strain H37Rv, we propose that variance in ROS production might contribute to immune evasion affecting DC maturation and antigen presentation.","palabras_clave":"ANTIGEN PRESENTATION|DENTRITIC CELLS|IMMUNE EVASION|MYCOBACTERIA|OXIDATIVE STRESS|TUBERCULOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3904,"idioma_id":4,"titulo":"DEVELOPMENT OF A MECHANISM AND AN ACCURATE AND SIMPLE MATHEMATICAL MODEL FOR THE DESCRIPTION OF DRUG RELEASE: APPLICATION TO A RELEVANT EXAMPLE OF ACETAZOLAMIDE-CONTROLLED RELEASE FROM A BIO-INSPIRED ELASTIN BASED HYDROGEL","resumen":"Transversality between mathematical modeling, pharmacology, and materials science is essential inorder to achieve controlled-release systems with advanced properties. In this regard, the area ofbiomaterials provides a platform for the development of depots that are able to achieve controlledrelease of a drug, whereas pharmacology strives to find new therapeutic molecules and mathematicalmodels have a connecting function, providing a rational understanding by modeling the parametersthat influence the release observed. Herein we present a mechanism which, based on reasonableassumptions, explains the experimental data obtained very well. In addition, we have developed asimple and accurate ?lumped? kinetics model to correctly fit the experimentally observed drugreleasebehavior. This lumped model allows us to have simple analytic solutions for the mass andrate of drug release as a function of time without limitations of time or mass of drug released,which represents an important step-forward in the area of in vitro drug delivery whencompared to the current state of the art in mathematical modeling. As an example, we appliedthe mechanism and model to the release data for acetazolamide from a recombinant polymer. Bothmaterials were selected because of a need to develop a suitable ophthalmic formulation for thetreatment of glaucoma. The in vitro release model proposed herein provides a valuable predictivetool for ensuring product performance and batch-to-batch reproducibility, thus paving the way for thedevelopment of further pharmaceutical devices.","palabras_clave":"Polymeric drug delivery systems|Acetazolamida|Biomaterials|Hydrogels"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3905,"idioma_id":4,"titulo":"Paclitaxel-Loaded TPGS-b-PCL Nanoparticles: In Vitro Cytotoxicity and Cellular Uptake in MCF-7 and MDA-MB-231 Cells versus mPEG-b-PCL Nanoparticles and Abraxane®","resumen":"Nanomedicines have become an attractive platform for the development of novel drug delivery systems in cancer chemotherapy. Polymeric nanoparticles (NPs) represent one of the best wellinvestigated nanosized carriers for delivery of antineoplastic compounds. The 'Pegylation strategy' of drug delivery systems has been used in order to improve carrier biodistribution, however, some nanosized systems with PEG on their surface have exhibited poorly-cellular drug internalization.  In this context, the purpose of the present study was to compare in vitro performance of two paclitaxel (PTX)-loaded NPs systems based on two biocompatible copolymers of alpha tocopheryl polyethylene glycol 1000 succinate-block-poly(-caprolactone) (TPGS-b-PCL) and methoxyPEGblock-poly(-caprolactone)  (mPEG-b-PCL) in terms of citotoxicity and PTX cellular uptake. Furthermore,  TPGS-b-PCL NPs were also copared with the commercially available PTX nano-sized  formulation Abraxane®. Both TPGS-b-PCL and mPEG-b-PCL derivates were synthesized by ring opening polymerization of -caprolactone employing microwaved radiation. NPs were obtained by a solvent evaporation technique where the PTX content was determined by reverse-phase HPLC. The resulting NPs had an average size between 200 and 300 nm with a narrow size distribution. Also both NPs systems showed a spherical shape. The in vitro PTX release profile from the NPs was characterized employing the dialysis membrane method where all drug-loaded formulations showed  a sustained and slow release of PTX. Finally, in vitro assays demonstrated that PTX-loaded TPGSb-PCL exhibited a significant higher antitumor activity than PTX-loaded mPEG-b-PCL NPs and Abraxane® against an estrogen-dependent (MCF-7) and an estrogen independent (MDA-MB-231) breast cancer cells lines. Furthermore TPGS-b-PCL NPs showed a significant increase on PTX cellular uptake, for both breast cell lines, in comparison with mPEG-b-PCL NPs and Abraxane®.  Overall findings confirmed that NPs based on TPGS-b-PCL as biomaterial demonstrated a better in vitro performance than NPs with PEG, representing an attractive alternative for the development of novel nanosized carriers for anticancer therapy.","palabras_clave":"PACLITAXEL|TPGS-b-PCL NANOPARTICLES|IN VITRO CYTOTOXICITY|CELLULAR UPTAKE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3906,"idioma_id":4,"titulo":"Novel Soluplus(®)-TPGS mixed micelles for encapsulation of paclitaxel with enhanced in vitro cytotoxicity on breast and ovarian cancer cell lines.","resumen":"The aim of this work was to develop mixed micelles based on two biocompatible copolymers of polyvinyl caprolactam?polyvinyl acetate?polyethylene glycol (Soluplus®) and D-&#945;-tocopheryl polyethylene-glycol 1000 succinate (TPGS), to improve the aqueous solubility and the in vitro anti-tumor activity of paclitaxel (PTX). Pure and mixed nanomicelles were prepared by solvent evaporation method and characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). Solubility of PTX was increased 60,000 and 38,000 times, when it was formulated in pure Soluplus® micelles and in mixed micelles (Soluplus®:TPGS; 4:1 ratio), respectively. The in vitro PTX release profile from micellar systems was characterized employing the dialysis membrane method where all drug-loaded formulations showed a sustained and slow release of PTX. In vitro assays were conducted on human cancer cell lines including ovarian cancer cells SKOV-3, breast cancer cells MCF-7 and triple negative breast cancer cells MDA-MB-231. Cytotoxicity studies showed that mixed micelles exhibited better antitumor activity compared to PTX solution against the three cell lines. Furthermore mixed micelles showed a significant increase on PTX cellular uptake in comparison with pure Soluplus® micelles and free drug in all cell lines assayed. More important, blank mixed micelles have shown cytotoxic activity due to the ability of TPGS to induce apoptosis in cancer cells. This effect was associated with the expression levels of cleaved-PARP, an apoptosis-related protein. On the basis of these results, the mixed micelles developed in this study might be a potential nano-drug delivery system for cancer chemotherapy.","palabras_clave":"TPGS|PACLITAXEL|BREAST CANCER|OVARIAN CANCER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3907,"idioma_id":4,"titulo":"Explicit estimates for the number of rational points of singular complete intersections over a finite field","resumen":"Let V&#8834;Pn(Fq)be a complete intersection defined over a finite field Fq of dimension r and singular locus of dimen-sion at most 0 &#8804;s &#8804;r&#8722;2. We obtain an explicit version of the Hooley-Katz estimate ||V(Fq)| &#8722;pr| =O(q(r+s+1)\/2), where |V(Fq)|denotes the number of Fq-rational points of Vand pr:=|Pr(Fq)|. Our estimate improves all the previous esti-mates in several important cases. Our approach relies on tools of classical algebraic geometry. A crucial ingredient is a new effective version of the Bertini smoothness theorem, namely an explicit upper bound of the degree of a proper Zariski closed subset of (Pn)s+1(Fq)which contains all the singular linear sections of V of codimension s +1.","palabras_clave":"Finite Fields|Singular complete Intersections|Rational points"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3908,"idioma_id":4,"titulo":"Case-based Reasoning for Web Service Discovery and Selection","resumen":"Web Service discovery and selection deal with the retrieval of the most suitable Web Service, given a required functionality. Addressing an effective solution remains difficult when only functional descriptions of services are available. In this paper, we propose a solution by applying Case-based Reasoning, in which the resemblance between a pair of cases is quantified through a similarity function. We show the feasibility of applying Case-based Reasoning for Web Service discovery and selection, by introducing a novel case representation, learning heuristics and three different similarity functions. We also experimentally validate our proposal with a dataset of 62 real-life Web Services, achieving competitive values in terms of well-known Information Retrieval metrics.","palabras_clave":"Web services|Service Selection|Service Discovery|Case-based Reasoning|Service Oriented Application"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3909,"idioma_id":4,"titulo":"RESTful Service Composition at a Glance: a Survey  (Indexed SCI, IF JCR 2014=2.229)","resumen":"In the last years, Web Service composition has undoubtedly become the most promising way to integrate business-to-business applications. However, the industry and the academia often disagree on materializing current solutions, which are based on either SOAP Web Services or semantic Web Services. Besides, any service composition mechanism entails multiple and complex factors such as adaptability, scalability and lightweightness. Recently, RESTful services have shown their potential to compose reliable and visible Web-scale applications based on the so-called mashups. In this paper, we survey a comprehensive set of RESTful composition approaches, i.e., the most promising in their area, totaling 29 approaches. Then, we propose two sets of features to analyze, characterize and compare such approaches: features inherent to SOAP services composition approaches and RESTful services composition features. Lastly, we discuss research challenges and open research problems in the area.","palabras_clave":"WEB SERVICES|RESTFUL SERVICES|SERVICE COMPOSITION|SERVICE MASHUPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3910,"idioma_id":4,"titulo":"Effect of roasting conditions on the antioxidant compounds of quinoa seeds","resumen":"The effect of different roasting conditions on the antioxidant properties and the phenolic content of quinoa seeds was studied. Advanced and final products of the Maillard reaction were also quantified in order to evaluate the contribution to DPPH radical scavenging capacity and reducing power of samples. In general, response surface analysis showed significant increases in the phenolic content, the antioxidant activity and the level of Maillard reaction products (MRPs), mainly as processing temperature increased, while roasting time had a minor impact on these response variables. The highest antioxidant activity was achieved in extracts of quinoa seeds roasted at 190&nbsp;°C for 30&nbsp;min. Principal component analysis applied to the data suggested that MRPs had a greater contribution to antioxidant properties than phenolic compounds in the processed samples. These results demonstrated that roasted quinoa seeds\/flour may be considered as a nontraditional ingredient with enhanced antioxidant capacity for the production of functional foods.","palabras_clave":"ANTIOXIDANT ACTIVITY|CHENOPODIUM QUINOA|MAILLARD REACTION PRODUCTS|PHENOLIC COMPOUNDS|ROASTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3912,"idioma_id":4,"titulo":"Global genomic diversity of human papillomavirus type 11 (HPV11) based on 433 isolates and 78 complete genome sequences","resumen":"Human papillomavirus 11 (HPV11) is an etiological agent of anogenital warts and laryngeal papillomas and is included in the 4-valent and 9-valent prophylactic HPV vaccines. We established the largest collection of globally circulating HPV11 isolates to date and examined the genomic diversity of 433 isolates and 78 complete genomes (CGs) from six continents. The genomic variation within the 2,800-bp E5a-E5b-L1-upstream regulatory region was initially studied in 181\/207 (87.4%) HPV11 isolates collected for this study. Of these, the CGs of 30 HPV11 variants containing unique single nucleotide polymorphisms (SNPs), indels (insertions or deletions), or amino acid changes were fully sequenced. A maximum likelihood tree based on the global alignment of 78 HPV11 CGs (30 CGs from our study and 48 CGs from GenBank) revealed two HPV11 lineages (lineages A and B) and four sublineages (sublineages A1, A2, A3, and A4). HPV11 (sub)lineage-specific SNPs within the CG were identified, as well as the 208-bp representative region for CG-based phylogenetic clustering within the partial E2 open reading frame and noncoding region 2. Globally, sublineage A2 was the most prevalent, followed by sublineages A1, A3, and A4 and lineage B.","palabras_clave":"HPV|HUMAN PAPILLOMAVIRUS|HPV11|GENOMIC DIVERSITY|GENETIC DIVERSITY|COMPLETE GENOME|DNA SEQUENCING|WORLDWIDE|GLOBAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3913,"idioma_id":4,"titulo":"Proinflammatory response of human trophoblastic cells to Brucella abortus infection and upon interactions with infected phagocytes","resumen":"Trophoblastsare a target of Brucella infection,but their role in the pathophysiology of the pregnancy complications ofbrucellosis is unknown. Here we show that B.abortus invades and replicates in the human trophoblastic cell line Swan-71,and that the intracellular survival of the bacterium depends on a functional virB operon. The infection elicitedsignificant increments of IL-8, MCP-1, and IL-6, but levels of IL-1&#946; and TNF-&#945;did not vary significantly. Such proinflammatory response was not modified bythe absence of the Brucella TIRdomain-containing proteins BtpA and BtpB. The stimulation of Swan-71 cells withconditioned medium (CM) from B. abortus-infectedhuman monocytes (THP-1 cells) or macrophages induced a significant increase of IL-8,MCP-1 and IL-6 as compared to stimulation with CM from non-infected cells. Similarresults were obtained when stimulation was performed with CM from infected neutrophils.Neutralization studies showed that IL-1&#946; and\/or TNF-&#945; mediated the stimulatingeffects of CM from infected phagocytes. Reciprocally, stimulation of monocytesand neutrophils with CM from Brucella-infectedtrophoblasts increased IL-8 and\/or IL-6 secretion. These results suggest thathuman trophoblasts may provide a local inflammatory environment during B. abortus infections either through adirect response to the pathogen or through interactions with monocytes\/macrophagesor neutrophils, potentially contributing to the pregnancy complications ofbrucellosis.","palabras_clave":"BRUCELLA|TROPHOBLAST|INFLAMMATORY RESPONSE|CELLULAR INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3914,"idioma_id":4,"titulo":"Synergistic effect of methyl jasmonate and cyclodextrins on anthraquinone accumulation in cell suspension cultures of Morinda citrifolia and Rubia tinctorum","resumen":"Plant in vitro culture is a platform for produc-ing secondary metabolites that combines safety, quality and low environmental impact. Besides, it is possible to increase the accumulation of these compounds by different strategies, such as elicitation. In this work, we analyzed the effects of the combination of methyl jasmonate (MeJ) and two cyclodextrins (CDs) on the production of anthraqui-nones (AQs) in cell cultures of Rubiaceae (Morinda citri-folia and Rubia tinctorum). These secondary metabolites have been traditionally used as dyes and have interesting therapeutic applications. The experiments were designed according to a full factorial design of two factors (MeJ and a CD) in two levels (0 and 0.1 mM for MeJ, and 0 and 20 mM of the CD). MeJ and (2-hydroxypropyl)-b-cy-clodextrin (HPCD) synergistically increased intracellular AQ content in suspension cultures of R. tinctorum, and, to a lesser extent, in suspension cultures of M. citrifolia. Combination of MeJ with another CD, 2-methyl-b-cy-clodextrin, led to a more intense and later increase in AQ content in cell cultures of R. tinctorum when compared to MeJ?HPCD treatment. However, the combination of CD and MeJ failed to induce a drastic AQ release to the culture media. Nevertheless, our results show that combination of strategies (using a CD and MeJ) was successful to increase secondary metabolite accumulation in suspension cultures. To our knowledge, this is the &#64257;rst report of synergistic effect of MeJ and CD on AQ accumulation in plant in vitro cultures.","palabras_clave":"Anthraquinones| Cyclodextrin|Elicitation|Methyl jasmonate"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3915,"idioma_id":4,"titulo":"Experimental and vdW-DFT Study of the Structure, Properties and Stability of Isonicotinic Acid Self-Assembled Monolayers on Gold","resumen":"Self-assembling of derivatized aromatic molecules on metals is of interest in the generation of modified surfaces with ionisable groups in order to generate charged surfaces for constructing more complex nanostructured interfaces. In the present contribution we explore the structure, properties and stability of isonicotinic acid (INA) adsorbed on gold surfaces employing electrochemical and spectroscopic methods as well as DFT calculations at the level of van der Waals interactions (vdW-DFT). Electrochemical results show changes in the kinetics of redox probes due to the self-assembled monolayers (SAMS) formation on the gold surface. Furthermore, SERS experiments indicate that INA species adsorb on gold perpendicularly to the surface through the deprotonated carboxylate group. In addition, due to the ionisable groups involved in the molecular anchorage, some instability of the SAMS of INA is obtained by pH modification of the chemical environment. On the other hand, theoretical calculations indicate that a strong interaction of the carboxylate group to the gold surface at the bridge sites takes place and the adsorbed species have sufficient mobility at room temperature. The effect of the coverage as well as the stability of the SAMS, are also reported.","palabras_clave":"SAMS|ISONICOTINIC ACID|AU(111)|SERS|VDW-DFT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3916,"idioma_id":4,"titulo":"Nanoencapsulation in the food industry: manufacture, applications and characterization","resumen":"Particle encapsulation is a standard process in the food industry that consists of encapsulating particles in a protective layer, covering, or containment material to protect a sensitive ingredient or nucleus from adversereactions. This consists of encapsulating small particle cores in a protective wall with the aim to preserveorganoleptic and physico-chemical properties of the original products as well as to improve the palatability ofvolatile odorous ingredients. Encapsulation of flavors and aromas is a rapidly expanding process in the foodindustry. Many aroma compounds must to be converted into solid products before its use as flavoring agents.Nano and microencapsulation technology is a very promising area in food industry which will have a great impacton a wide variety of products including functional foods, packaging, preservatives, antioxidants, flavors andfragrances. Spray Drying is a widely used technique to eliminate water and other solvents from solutions, emulsionsand suspensions. Spray drying is a rapid, convenient and low cost method in comparison to other techniques aslyophilization. This technology is one of the most common encapsulation methods used in the food industrybecause of its advantages, such as the wide availability of equipment, large-scale production, simple continuousunit operation, ease of manipulation, and low process cost.","palabras_clave":"NANOTECHNOLOGY|FOOD SCIENCE|ENCAPSULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3917,"idioma_id":4,"titulo":"Spray dried microspheres based on chitosan: A promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis","resumen":"Previous studies have demonstrated that parenteral immunization with polymeric antigen BLSOmp31 induced a strong immune response and conferred protection against Brucella ovis in rams. This work describes the development of a novel formulation strategy for the delivery of BLSOmp31 in the nasal mucosa. Chitosan microparticles were prepared by spray-drying technology processes and recombinant chimera BLSOmp31 was loaded by passive adsorption onto chitosan microspheres, which were characterized by means of the evaluation of size, zeta potential, morphology, and loading and release rate of BLSOmp31. The mucoadhesive properties of microspheres were evaluated by studying the interaction between microparticles and mucin. The antigen BLSOmp31 integrity was investigated by SDS-PAGE. The yield of production of spray-drying process was 68.95%. Microspheres had a good sphericity, 1?10 &#956;m of particle size and had a positive charge. The loading capacity was found to be 45.19%. The initial fast release of BLSOmp31 from chitosan microparticles was 60%. The BLSOmp31 integrity was not affected by passive adsorption (ionic interaction). The amount of mucin adsorbed on the surface of CMs-BLSOmp31 was lower than on the surface of blank CMs at neutral pH. In vivo studies were carried out in rams. Intranasal immunization induced systemic and local antibodies. In conclusion, the use of BLSOmp31-loaded chitosan spray-drying microspheres offers a promising way for nasal mucosal vaccination in sheep against brucellosis.","palabras_clave":"Chitosan microspheres|Spray-drying|Passive adsorption|BLSOmp31|Intranasal immunization|Systemic and local antibodies"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3918,"idioma_id":5,"titulo":"Producción potencial de semillas por planta en especies de Bromus (Sección Ceratochloa), en el sudeste de la provincia de Buenos Aires","resumen":"En Argentina crecen varias especies de la Sección Ceratochloa del género Bromus,que se destacan por su producción de forraje inverno-primaveral. El valor de estos recursos nativos ha incentivado la colecta de semillasde diferentes poblaciones las que se conservan en Bancos de Germoplasma para su eventual utilización en la obtención devariedades de alta producción de forraje y\/o semilla, previaevaluación de sus características. Con el objetivo de determinar la producción potencial de semillas por planta mediante los componentes de rendimiento, se cultivaron en Balcarce 10 poblaciones pertenecientes a B.bonariensis, B.coloratus, B.parodii y B.catharticus (variedades: catharticus,rupestrisy elata), y dos cultivares (Gato y Volcán), según un diseño en bloques completos con n=4 y 12 plantas\/parcela. Se registró el número de panojas\/planta,de espiguillas\/panoja y de flores\/espiguilla, y se estimó el número potencial de semillas por panoja y planta, peso de 1000 semillas yrendimiento (g\/panoja y g\/planta). Se realizó análisis de la varianza y comparación de tratamientos con DMS (p&lt;0,05). Se detectó variabilidad significativa para varios atributos. Los materiales genéticos no difirieron en el número de semillas\/panoja ante la compensación establecida entre sus componentes, pero sí a nivel planta, debido principalmente a la cantidad diferencial de panojas\/planta. Hubo compensación entre peso y número de semillas, pero igualmente los materiales presentaron diferencias en rendimiento de semillas por planta. Éste fue mayor en los cultivares y en dos poblaciones silvestres pertenecientes a B.bonariensis y a B. catharticus var.catharticus, las que podrían considerarse materiales promisorios para su inclusión en planes de mejoramiento.","palabras_clave":"CEBADILLAS|EVALUACION|POBLACIONES|RENDIMIENTO|VARIABILIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3919,"idioma_id":4,"titulo":"Small Peptides Derived from Penetratin as Antibacterial Agents","resumen":"The synthesis, in vitro evaluation and conformational study of several small-size peptides acting as antibacterial agents are reported. Among the compounds evaluated, the peptides Arg-Gln-Ile-Lys-Ile-Trp-Arg-Arg-Met-Lys-Trp-Lys-Lys-NH2, Arg-Gln-Ile-Lys-Ile-Arg-Arg-Met-Lys-Trp-Arg-NH2, and Arg-Gln-Ile-Trp-Trp-Trp-Trp-Gln-Arg-NH2 exhibited significant antibacterial activity. These were found to be very active antibacterial compounds, considering their small molecular size. In order to better understand the antibacterial activity obtained for these peptides, an exhaustive conformational analysis was performed, using both theoretical calculations and experimental measurements. Molecular dynamics simulations using two different media (water and trifluoroethanol\/water) were employed. The results of these theoretical calculations were corroborated by experimental circular dichroism measurements. A brief discussion on the possible mechanism of action of these peptides at molecular level is also presented. Some of the peptides reported here constitute very interesting structures to be used as starting compounds for the design of new small-size peptides possessing antibacterial activity.","palabras_clave":"Antibacterial activity|Peptide syntheses|Rational drug design"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3920,"idioma_id":4,"titulo":"Exploring children's thinking with and about numbers from a resources-based approach","resumen":"The aim of this study is to achieve a deeper understanding of therepertoire of cognitive resources children can display in the process oflearning numbers. Forty-two children attending Age 4 KindergartenClass or Year-One in Argentina were individually interviewed, based on asemi-structured script requiring them to represent definite andindefinite quantities orally and notationally. Children?s responsesthroughout the interview were categorized according to Scope ofconventional knowledge, Non-conventional responses, Playfulness andProgress. Results of a series of Classification Analyses showed three mainclasses, which capture the tension between using what is known andexploring novelties in order to represent numerical meanings","palabras_clave":"NUMBER|CHILDREN|COGNITIVE|RESOURCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3921,"idioma_id":4,"titulo":"Rotation control of a parametrically excited pendulum by adjusting its length","resumen":"We present a new control strategy for the vertically excited parametric pendulum, with a view on energy harvesting from rotating motion. Two possible energy sources are considered: a vibrating machine, represented by a sinusoidal excitation; and the sea waves, simulated by a stochastic process. In both cases, rotations can be achieved and maintained only for some forcing conditions. Thus, as stable rotations are required, the pendulum must be controlled. We propose to perform this control by means of a telescopic adjustment of the pendulum length during the motion. The idea is to give the pendulum an aid to reach and maintain rotations, accelerating the motion by modifying conveniently the position of the mass. To a better understanding of this concept, one may think of a child on a swing, who extends or retracts his legs in order to accelerate the motion. Numerical simulations show that, with a control action of this kind, stable rotations can be reached regardless of the forcing conditions and for every set of initial conditions. These are very promising results in terms of energy harvesting, since an optimized application of this control technique can lead to the design of autonomous pendulum harvester devices.","palabras_clave":"Energy harvesting|Parametric pendulum|Length adjustment|Wave energy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3922,"idioma_id":4,"titulo":"Embryo aggregation in pig improves cloning efficiency and embryo quality","resumen":"In this study, we analyzed the effects of the cloned embryo aggregation on in vitro embryo development and embryo quality by measuring blastocyst diameter and cell number, DNA fragmentation levels and the expression of genes associated withpluripotency, apoptosis, trophoblast and DNA methylation in the porcine. Zona­free reconstructed cloned embryos were cultured inthe well of the well system, placing one (1x non aggregated group) or three (3x group) embryos per microwell. Our results showedthat aggregation of three embryos increased blastocyst formation rate and blastocyst diameter of cloned pig embryos. DNAfragmentation levels in 3x aggregated cloned blastocysts were significantly decreased compared to 1x blastocysts. Levels of Oct4,Klf4, Igf2, Bax and Dnmt 1 transcripts were significantly higher in aggregated embryos, whereas Nanog levels were not affected.Transcripts of Cdx2 and Bcl­xl were essentially non­detectable. Our study suggests that embryo aggregation in the porcine may bebeneficial for cloned embryo development and embryo quality, through a reduction in apoptotic levels and an improvement in cellreprogramming.","palabras_clave":"embryo quality|SCNT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3923,"idioma_id":4,"titulo":"Modified bacterial cellulose scaffolds for localized doxorubicin release in human colorectal HT-29 cells","resumen":"Bacterial cellulose (BC) films modified by the in situ method with the addition of alginate (Alg) during the microbial cultivation of Gluconacetobacter hansenii under static conditions increased the loading of doxorubicin by at least three times. Biophysical analysis of BC-Alg films by scanning electron microscopy, thermogravimetry, X-ray diffraction and FTIR showed a highly homogeneous interpenetrated network scaffold without changes in the BC crystalline structure but with an increased amorphous phase. The main molecular interactions determined by FTIR between both biopolymers clearly suggest high compatibility. These results indicate that alginate plays a key role in the biophysical properties of the hybrid BC matrix. BC-Alg scaffold analysis by nitrogen adsorption isotherms revealed by the Brunauer?Emmett?Teller (BET) method an increase in surface area of about 84% and in pore volume of more than 200%. The Barrett?Joyner?Halenda (BJH) model also showed an increase of about 25% in the pore size compared to the BC film.Loading BC-Alg scaffolds with different amounts of doxorubicin decreased the cell viability of HT-29 human colorectal adenocarcinoma cell line compared to the free Dox from around 95?53% after 24 h and from 63% to 37% after 48 h. Dox kinetic release from the BC-Alg nanocomposite displayed hyperbolic curves related to the different amounts of drug payload and was stable for at least 14 days. The results of the BC-Alg nanocomposites show a promissory potential for anticancer therapies of solid tumors.","palabras_clave":"Bacterial cellulose|Alginate|Drug delivery|Nanocomposite"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3924,"idioma_id":4,"titulo":"Discovery of a loose star cluster in the Large Magellanic Cloud","resumen":"We present results for an up-to-date uncatalogued star cluster projectedtowards the Eastern side of the Large Magellanic Cloud (LMC) outer disc.The new object was discovered from a search of loose star cluster in theMagellanic Clouds´ (MCs) outskirts using kernel density estimators on Washington$CT_1$ deep images. Contrarily to what would be commonly expected, thestar cluster resulted to be a young object (log($t$ yr$^{-1}$) = 8.45) witha slightly subsolar metal content ($Z$ = 0.013) and a total mass of 650$msun$.Its core, half-mass and tidal radii also are within the frequent values ofLMC star clusters.However, the new star cluster is placed at the Small Magellanic Cloud distance and at 11.3 kpc from the LMC centre. We speculate with the possibility thatit was born in the inner body of the LMC and soon after expeled into the intergalactic space during the recent Milky Way\/MCs interaction.Nevertheless,radial velocity and chemicalabundance measurements are needed to further understand its origin, as well asextensive search for loose star cluster in order to constrain the effectiveness ofstar cluster scattering during galaxy interactions.","palabras_clave":"cluster"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3925,"idioma_id":4,"titulo":"An Oral Salmonella-Based Vaccine Inhibits Liver Metastases by Promoting Tumor-Specific T-Cell-Mediated Immunity in Celiac and Portal Lymph Nodes: A Preclinical Study","resumen":"Primary tumor excision is one of the most widely used therapies of cancer. However, the risk of metastases development still exists following tumor resection. The liver is a common site of metastatic disease for numerous cancers. Breast cancer is one of the most frequent sources of metastases to the liver. The aim of this work was to evaluate the efficacy of the orally administered Salmonella Typhi vaccine strain CVD 915 on the development of liver metastases in a mouse model of breast cancer. To this end, one group of BALB\/c mice was orogastrically immunized with CVD 915, while another received PBS as a control. After 24&nbsp;h, mice were injected with LM3 mammary adenocarcinoma cells into the spleen and subjected to splenectomy. This oral Salmonella-based vaccine produced an antitumor effect, leading to a decrease in the number and volume of liver metastases. Immunization with Salmonella induced an early cellular immune response in mice. This innate stimulation rendered a large production of IFN-&#947; by intrahepatic immune cells (IHIC) detected within 24&nbsp;h. An antitumor adaptive immunity was found in the liver and celiac and portal lymph nodes (LDLN) 21&nbsp;days after oral bacterial inoculation. The antitumor immune response inside the liver was associated with increased CD4+ and dendritic cell populations as well as with an inflammatory infiltrate located around liver metastatic nodules. Enlarged levels of inflammatory cytokines (IFN-&#947; and TNF) were also detected in IHIC. Furthermore, a tumor-specific production of IFN-&#947; and TNF as well as tumor-specific IFN-&#947;-producing CD8 T cells (CD8+IFN-&#947;+) were found in the celiac and portal lymph nodes of Salmonella-treated mice.This study provides first evidence for the involvement of LDLN in the development of an efficient cellular immune response against hepatic tumors, which resulted in the elimination of liver metastases after oral Salmonella-based vaccination","palabras_clave":"SALMONELLA TYPHI|VACCINE|LIVER METASTASES|BREAST CANCER|ANTITUMOR CD8+ T CELLS|TH1 IMMUNE RESPONSE|CELIAC AND PORTAL LYMPH NODES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3926,"idioma_id":4,"titulo":"CANNIBALISM IN NATICIDAE FROM THE LA MESETA FORMATION (EOCENE, ANTARCTICA)","resumen":"Polinices marambioensis Stilwell & Zinsmeister, is a naticid gastropod which is the most common constituent in fossil accumulations in the upper section of the Cucullaea I Allomember (Middle Eocene), of the La Meseta Formation, in the James Ross Basin of the Antarctic Peninsula. This species was an important predator of infaunal bivalves and gastropods, including other naticids. The aim of this work is to assess the pattern of predation and cannibalistic behavior of P. marambioensis. A total of 2,648 specimens of P. marambioensis were examined for drill holes, which were assigned to Oichnus paraboloides Bromley. Drilling frequency data were measured as a proxy for predation intensity and statistical analyses were performed. Also site of each drill hole was established according to the morphological features of the shell on each specimen to assess possible preference of predators for the site of perforation. Results suggest that P. marambioensis is an efficient cannibalistic predator for a specific size range of prey (8-22 mm), and drill holes are distributed preferentially in two specific sectors of their shells. This selective cannibalistic prey behavior in P. marambioensis affected not only the dynamics of their populations but the ecological structure of the community in which they lived.","palabras_clave":"GASTROPODS|DRILLING TRACES |PREDATION|PREDATOR-PREY BEHAVIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3927,"idioma_id":4,"titulo":"Individual restriction of fine specificity variability in anti-GM1 IgG antibodies associated with Guillain-Barré Syndrome","resumen":"Elevated titers of serum antibodies against GM1 ganglioside are associated with a variety of autoimmune neuropathies. Although much evidence indicates these autoantibodies play a primary role in the disease processes, the mechanism of their appearance is unclear. We studied the fine specificity of anti-GM1 antibodies of the IgG isotype present in sera from patients with Guillain-Barré syndrome (GBS). Using thin-layer chromatogram-immunostaining of GM1, asialo-GM1, GD1b and GM1-derivatives with small modifications on the oligosaccharide moiety, we were able to distinguish populations of antibodies with different fine specificity. Remarkably, individual patients presented only one or two of them, and different patients had different populations. This restriction in the variability of antibody populations suggests that the appearance of the anti-GM1 antibodies is a random process involving restricted populations of lymphocytes. With the origin of disease-associated anti-GM1 antibodies as a context, this finding could provide explanation for the ?host susceptibility factor? observed in GBS following enteritis with GM1 oligosaccharide-carrying strains of Campylobacter jejuni.","palabras_clave":"Anti-GM1 antibody|fine specificity|Guillain-Barré syndrome"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3928,"idioma_id":4,"titulo":"Evaluation of the status of rotary machines by time causal Information Theory quantifiers","resumen":"In this paper several causal Information Theory quantifiers: Shannon entropy, statistical complexity and Fisher information measure are applied to describe the behavior of a rotating machine. An experiment was conducted where a rotating machine run balanced and then, after a misalignment,  run unbalanced.  All the causal Information Theory quantifier applied are capable  to distinguish between both states and grasp the corresponding transition between them.","palabras_clave":"Permutation entropy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3929,"idioma_id":4,"titulo":"The role of Phyllocaulis variegatus (Mollusca: Veronicellidae) in the transmission of digenean parasites","resumen":"Ninety five veronicellid slugs identified as Phyllocaulis variegatus were collected in Puerto Iguazu, Misiones Province, Argentina. Specimens were examined for parasites. Metacercariae of the genus Brachylaima(Brachylaimidae) were recovered from the body cavity near the female genital pore. The results establish the capability of P.variegatus to act as an intermediate host of Brachylaimasp.in the area.This study represents the first record of metacercariae of Brachylaima sp. in Argentina.","palabras_clave":"Brachylaima sp|Metacercariae|Phyllocaulis variegatus|Intermediate host|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3930,"idioma_id":4,"titulo":"New host, geographic records, and histopathologic studies of Angiostrongylus spp (Nematoda: Angiostrongylidae) in rodents from Argentina with updated summary of records from rodent hosts and host specificity assessment","resumen":"To date, 21 species of the genus Angiostrongylus (Nematoda: Angiostrongylidae) have been reported around theworld, 15 of which are parasites of rodents. In this study, new host, geographic records, and histopathologic studies ofAngiostrongylus spp in sigmodontine rodents from Argentina, with an updated summary of records from rodent hostsand host specificity assessment, are provided. Records of Angiostrongylus costaricensis from Akodon montensis andAngiostrongylus morerai from six new hosts and geographical localities in Argentina are reported. The gross and histopathologicchanges in the lungs of the host species due to angiostrongylosis are described. Published records of thegenus Angiostrongylus from rodents and patterns of host specificity are presented. Individual Angiostrongylus speciesparasitise between one-19 different host species. The most frequent values of the specificity index (STD) were between1-5.97. The elevated number of host species (n = 7) of A. morerai with a STD = 1.86 is a reflection of multiple systematicstudies of parasites from sigmodontine rodents in the area of Cuenca del Plata, Argentina, showing that an increase insampling effort can result in new findings. The combination of low host specificity and a wide geographic distribution ofAngiostrongylus spp indicates a troubling epidemiological scenario although, as yet, no human cases have been reported.","palabras_clave":"Agiostrogylus|Hustopathology|host specificity|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3931,"idioma_id":4,"titulo":"Expression of the  Sinorhizobium meliloti small RNA gene mmgR is controlled by the nitrogen source","resumen":"Smallnon-coding RNAs (sRNAs) are key players in post-transcriptional regulation ofgene expression. Hundreds of sRNAs have been identified in Sinorhizobium meliloti but their biological function remainsunknown for most of them. In this study, we characterized the expressionpattern of the gene encoding the 77-nt sRNA MmgR in S. meliloti strain 2011. A chromosomal transcriptional reporterfusion (PmmgR-gfp) showed that the mmgRpromoter is active along different stages of the interaction with alfalfa roots.In vitro, PmmgR-gfp activityparalleled the sRNA abundance indicating that mmgR expression is primarily controlled at the level oftranscriptional initiation. PmmgR-gfp activity was higher during growth inRDM medium than in TY medium. Furthermore, PmmgR-gfp was induced at 60 min after shiftinggrowing cells from TY to RDM medium, i.e., faster than the cell doubling time.In defined RDM medium containing NO3-, both PmmgR-gfpand MmgR level were repressed by addition of tryptone or single amino acids,suggesting that mmgR expressiondepends on the cellular N status. Insilico analysis failed to detect conserved motifs upstream the promoter RNApolymerase binding site, but revealed a strongly conserved motif centered at-28, which may be linked to the observed regulatory pattern by the nitrogensource.","palabras_clave":"Sinorhizobium meliloti|sRNA|Expression|Nitrogen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3933,"idioma_id":4,"titulo":"Product Development Process in food industries: Tools management analysis in post-development stage","resumen":"Product Development Process (PDP) is considered increasingly important to the competitiveness of businessprocedure, especially at food producing industries.Some authors propose a model that outlines the process in macrophases. It underscores Post-Developmentmacrophase, which involves following the products on the market, from launch to its recall.This paper proposes procedures and tools to be used at Post-development macrophase of PDP in food industries.The study is based on a literature review, in relation to the PDP in food producing industries and also to PDP andmanagement tools.Among the main conclusions, it stresses that in food industries a large number of procedures and tools are related tothe activities of Development stage. Regarding Post-development macrophase, the methodologies used are mainlyfocused on customer satisfaction activities, due to its importance for companies, but is still incipient the use ofmodels that standardize the management of the PDP at this stage.","palabras_clave":"DEVELOPMENT|PRODUCT|MANAGEMENT|TOOLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3934,"idioma_id":4,"titulo":"Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds","resumen":"tThe antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant(CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three differentM. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to thoseof CFS and LF, regardless of the fungal strain studied. The synthesis of lipopeptides was studied by UV-MALDI TOF. Chemical analysis of Bacillus metabolite synthesis revealed that surfactin and iturin weremainly produced in liquid medium. Potential fengycin was also co-produced when both Bacillus werecultivated in solid medium. In co-culture assays, the bacterial colony-fungal mycelium interface at theinhibition zone was evaluated by both scanning electron microscopy (SEM) and UV-MALDI TOF, theformer to determine the structural changes on M. phaseolina cells and the latter to identify the mainbioactive molecules involved in the inhibitory effect. PGPBacCA1 produced surfactin, iturin and fengycinin the inhibition zone while PGPMori7 only produced these metabolites within its colony and not in thenarrow inhibition zone. Interestingly, SEM revealed that PGPBacCA1 induced damage in M. phaseolinasclerotia, generating a fungicidal effect as no growth was observed when normal growth conditions werereestablished. In turn, PGPMori7 inhibited the growth of the Macrophomina mycelium without fungalinjury, resulting only in a fungistatic activity. From these results, it was determined that the two bacillisignificantly inhibited the growth of an important phytopathogenic fungus by at least two differentmechanisms: lipopeptide synthesis and competition among microorganisms.© 2015 Elsevier GmbH. All rights reserved.","palabras_clave":"MALDI-MS|Bacillus subtilis |Bacillus |MALDI matrix"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3935,"idioma_id":4,"titulo":"Energetics of neutral and deprotonated (Z)-cinnamic acid","resumen":"We have performed a study of structural, thermochemical and thermophysical properties of the(Z)-cinnamic acid neutral molecule and its corresponding oxyanion (formed by deprotonation of thecarboxylic group). The thermophysical study (heat capacities, temperature and enthalpy of fusion) wasmade by DSC. The following intrinsic (gas-phase) thermochemical magnitudes have been experimentallydetermined: (i) standard enthalpy of formation, at T = 298.15 K, of the neutral molecule, DfH0m(g) =(215.5 ± 3.2) kJ  mol1, by combustion calorimetry and by the Knudsen effusion technique, (ii) deprotonationenthalpy, DacidH0(g) = (1416.4 ± 8.8) kJ  mol1 and acidity, GA = (1386.7 ± 8.8) kJ  mol1, by theEKM method using ESI-TQ Mass Spectrometry. From these results we have also derived the enthalpy offormation of the oxyanion, DfH0m(oxyanion, g) = (303.5 ± 9.4) kJ  mol1. A computational study, throughdensity functional calculations at the B3LYP\/6-311++G(d,p) level of theory, was used to check the goodconsistency of the experimental results. The global results show that (Z)-cinnamic acid is significantlyless stable than the corresponding (E)-isomer, which can be related to the greater acidity of the(Z)-form found in both the gas and aqueous solution phases.","palabras_clave":"cinamicos|E-cinamico|Z-cinamico|termica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3936,"idioma_id":4,"titulo":"Chemical and photochemical properties of chloroharmine derivatives in aqueous solutions","resumen":"Thermal and photochemical stability (FR), room temperature UV-vis absorption and fluorescencespectra, fluorescence quantum yields (FF) and lifetimes (tF), quantum yields of hydrogen peroxide (FH2O2)and singlet oxygen (FD) production, and triplet lifetimes (tT) have been obtained for the neutral andprotonated forms of 6-chloroharmine, 8-chloroharmine and 6,8-dichloroharmine, in aqueous media.When it was possible, the effect of pH and oxygen concentration was evaluated. The nature ofelectronic transitions of protonated and neutral species of the three investigated chloroharmines wasestablished using Time-Dependent Density Functional Theory (TD-DFT) calculations. The impact of allthe foregoing observations on the biological role of the studied compounds is discussed.","palabras_clave":"Fotoquimica|carbolinas|Cl-carbolinas|Fotosenisibilizadores"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3937,"idioma_id":4,"titulo":"Formation of 2,2-dimethylchroman-4-ones during the photoinduced rearrangement of some aryl 3-methyl-2-butenoate esters. A mechanistic insight","resumen":"Several aryl 3-methyl-2-butenoate esters upon irradiation lead to the formation of [1,3]-migrated photoproducts,phenol and, surprisingly 2,2-dimethylchroman-4-one derivatives. The starting photochemicalreaction takes place from the singlet excited state of the ester and as a total mechanism twoconsecutive reaction pathways are proposed. The former involves the photo-Fries rearrangement of theesters in all the solvents studied and, depending on the proticity of the solvent, the latter involves anESIPT process followed by thermal 6p-electrocyclic reaction and\/or thermal (intramolecular oxa-Michaeladdition) cyclization of the ortho-regioisomers photoformed. This second pathway is responsible of theformation of the 2,2-dimethylchroman-4-one derivatives","palabras_clave":"2,2-Dimethylchroman-4-ones|photo-Fries rearrangement|Aryl 3-methyl-2-butenoate esters|Thermal 6p-electrocyclic reaction|oxa-Michael cyclization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3938,"idioma_id":4,"titulo":"Nanotoxicological effects of SiO2 nanoparticles on Spodoptera frugiperda Sf9 cells","resumen":"The application of silica nanoparticles (NPs) in the biomedical field experienced a great development. The driving forces for these and future developments are the possibility to design NPs with homogeneous size and structure amenable to specific grafting. Moreover, it is possible to tune the characteristics of the NPs to meet the requirements of each specific cell and desired application. Herein, we analyzed the effect of silica NPs of various sizes and surface charge on the viability of Spodoptera frugiperda cells (Sf9 cell line) with the aim of extending the knowledge of possible toxicity of the NPs in the environment and development of new tools for insect control. Moreover, these results will also contribute to develop more effective systems for gene vectors delivery and recombinant proteins expression. Bare silica NPs of 14 nm, 380 nm and 1430 nm as well as amine-modified silica NPs of 131 nm and 448 nm were obtained by the Stöber method. The NPs were characterized by DLS and zeta potential measurements. The cell viability was assessed by the MTT test. It was observed that the 14 nm NPs possess the highest toxic effect. Indeed, after 24h, the viability of the cells exposed to the lower concentration of NPs (0.12 mg\/ml) was about 40% of the value obtained for the control cells not exposed to NPs. Moreover, the exposure to other negative charged NPs also causes a lower activity when compared with the control. Alternatively, lower concentrations of positive charged NPs (i.e.: 0.12 or 0.6 mg\/ml) demonstrated to stimulate the proliferation of the cells and higher concentrations (i.e.: 7.2 mg\/ml) did not present significant differences with the control. In conclusion, we have demonstrated that the NPs possess an effect that is highly influenced by the size, charge and concentration. Although, silica NPs are being used in the biomedical field, these results contribute to further understanding the risk that could be associated to nanoparticles and how these can be modified in order to meet the requirements of each desired application.","palabras_clave":"nanotoxicologia|nanoparticulas|células de insecto"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3939,"idioma_id":4,"titulo":"Study Conception Rate and its Relationship with Motility Morphology Sperm and Holstein Dairy Cows to Pasture  Systems","resumen":"The objective was to analyze the results of fertility and relate morphology and number of sperm of bulls used in artificial insemination in grazing systems. 12780 backdate services were evaluated with 43 bulls to heifers and cows made from three dairy farms located in the province of Entre Rios - Argentina during the period 2011-2014. The variables evaluated were: sperm morphology, motile spermatozoa per dose, conception rate and number of services per pregnancy. The differences with statistical significance (P &#8804; 0.05) were observed in the number of services that were needed to achieve pregnancy and conception rate. The variables taking into account the time of year when services are performed emerged that at the time from the beginning of autumn until spring, the amount of services required by cows and heifers was significantly lower (p &#8804; 0.05) and conception rate also increased significantly (p&#8804;0,5) if these variables with the results obtained during the summer are compared. It is concluded that conception rate is not related to the morphology and sperm motility in Holstein dairy cows in systems grazing, however if the time of year in which the services were performed, being less beneficial to service in the months where the ambient temperature he begins to rise.","palabras_clave":"dairy cow|conception rate|sperm morphology and sperm motility|grazing system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3940,"idioma_id":4,"titulo":"Immune response induced by conjunctival immunization with polymeric antigen BLSOmp31 using a thermoresponsive and mucoadhesive in situ gel as vaccine delivery system for prevention of ovine brucellosis","resumen":"Control of ovine brucellosis with subcellular vaccines can solve some drawbacks associated with the useof Brucella melitensis Rev.1. Previous studies have demonstrated that the polymeric antigen BLSOmp31administered by parenteral route was immunogenic and conferred significant protection against B. ovisin rams. Immunization with BLSOmp31 by conjunctival route could be efficient for the induction ofmucosal and systemic immune responses. In this work, we evaluated the conjunctival immunizationusing a thermoresponsive and mucoadhesive in situ gel composed of Poloxamer 407 (P407) and chitosan(Ch) as vaccine delivery system for BLSOmp31 in rams. Serum samples, saliva, lacrimal, preputial andnasal secretions were analyzed to measure specific IgG and IgA antibodies. Cellular immune responsewas evaluated in vivo and in vitro. Immunization with BLSOmp31-P407-Ch induced high IgG antibodylevels in serum and preputial secretions which remained at similar levels until the end of the experiment.Levels of IgG in saliva, lacrimal and nasal secretions were also higher compared to unvaccinated controlgroup but decreased more rapidly. IgA antibodies were only detected in nasal and preputial secretions.BLSOmp31-P407-Ch stimulated a significant cellular immune response in vivo and in vitro. The inductionof systemic and local immune responses indicates a promising potential of P407-Ch for the delivery ofBLSOmp31 by conjunctival route.","palabras_clave":"BLSOmp31-Poloxamer407-Chitosan|conjunctival immunization|lambs|immunogenicity|Brucella ovis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3941,"idioma_id":4,"titulo":"Development of a Construct-Based Risk Assessment Framework for Genetic Engineered Crops","resumen":"Experiencegained in the risk assessment (RA) of genetically engineered (GE) crops sincetheir first experimental introductions in the early nineties, has increased thelevel of familiarity with&nbsp; these breedingmethodologies and has motivated several agencies and expert groups worldwide torevisit the scientific criteria underlying the RA process. Along these lines,the need to engage in a scientific discussion for the case of GE cropstransformed with similar constructs was recently identified in Argentina. Inresponse to this need, the Argentine branch of the International Life SciencesInstitute (ILSI Argentina) convened a tripartite working group &nbsp;(WG) to discuss&nbsp; a science-based evaluation approach fortransformation events developed with genetic constructs which are identical orsimilar to those used in previously evaluated or approved GE crops. This discussion considered new transformation events within the sameor different species and covered both environmental and food safety aspects.&nbsp; A construct similarity concept was defined,considering the biological function of the introduced genes.&nbsp; Factors like environmental and dietaryexposure, familiarity with both the crop and the trait as well as the cropbiology, were identified as key to inform a construct-based RA process.","palabras_clave":"Genetic engineering|Familiarity|Construct similarity|Problem Formulation|Risk Assessment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3942,"idioma_id":4,"titulo":"Nanoparticles of tungsten as low-cost monometallic catalyst for selective hydrogenation of 3-hexyne","resumen":"Low cost tungsten monometallic catalysts with variable amounts of metal (4.5, 7.1 and 8.5 %W) were prepared by impregnating alumina with ammonium metatungstate as an inexpensive precursor. The catalysts were characterized using ICP, XPS, XRD, TPR and hydrogen chemisorption. These techniques revealed mainly WO3-Al2O3 (W6+) species on the surface.The effects of the content of W nanoparticles and the reaction temperature on the activity and selectivity for the partial hydrogenation of 3-hexyne, a non terminal alkyne, were assessed at moderate conditions of temperature and pressure.The prepared monometallic catalysts were found to be active and stereoselective for the production of (Z)-3-hexene, showing the following order: 7.1WN\/A > 8.5 WN\/A &#8805; 4.5 WN\/A. Additionally, the performance of the synthesized xWN\/A catalysts exhibited a high sensitivity to the variation of temperature. In all the cases, the maximum 3-hexyne total conversion and selectivity was achieved at 323 K.The performance of the catalysts was considered to be a consequence of two phenomena: a) the electronic effects, related to the high charge of W (+6), causing an intensive dipole moment in the hydrogen molecule (van der Waals forces) and leading to a heterolytic bond rupture; the generated H+ and H- species would approach a 3-hexyne adsorbate molecule and would provoke the heterolytic rupture of the C&#61626;C bond into C- = C+; and b) steric effects related to the high concentration of WO3 on 8.5WN\/A that would block the Al2O3 support. Catalyst deactivation was detected, starting at about 50 min of reaction time. Electrodeficient W6+ species would be responsible for the formation of green oil at the surface level, blocking pores and active sites of the catalyst, particularly at low reaction temperatures (293 and 303 K).The resulting best catalyst, 7.1WN\/A, has both a low cost of fabrication and a high selectivity to (Z)-3-hexene (94%) at 323 K. This selectivity is comparable to that of the classical and more expensive industrial Lindlar catalyst (5 wt% Pd).The alumina supported tungsten catalysts could be low-cost potential replacements of the Lindlar industrial catalyst. They could also be used for preparing bimetallic W-Pd catalysts for selective hydrogenation of terminal and non-terminal alkynes.","palabras_clave":"TUNGSTEN|MONOMETALLIC CATALYST|SELECTIVE HYDROGENATION|NON-TERMINAL ALKYNE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3943,"idioma_id":4,"titulo":"Numerical study of gas mixture separation in curved nozzles","resumen":"Species separation can be produced by imposing a pressure gradient in gaseous mixtures, which induces different molecular velocities depending on the molar weight. Pressure gradients can be achieved by centrifugal forces brought about by the passage of the gas through a curved nozzle at supersonic velocity. The efficiency of this process depends on the geometry of the nozzle as well as the flow operating conditions. The numerical solver Fluent was used in order to produce a model of the aerodynamics and the oxygen diffusion of a steady-state flow of air in a curved nozzle. The development of the pressure and O2concentration profiles along the nozzle were analyzed for different pressure boundary conditions at the inlet and the exit, testing several nozzle sizes. Optimum values of the cut and the inlet pressure were found which maximize the separation efficiency. The effect of the exit pressure was associated with the axial pressure distribution along the inner wall of the nozzle. The results were compared with measurements showing good agreement.","palabras_clave":"Curved nozzle|Species separation|Supersonic flow|Separative capacity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3944,"idioma_id":4,"titulo":"Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires province, Argentina","resumen":"In order to determine the prevalence and risk factors for shedding of Cryptosporidium spp. in diary calves a cross-sectional study was carried out in the northeast region of Buenos Aires province, Argentina. Fecal samples from a total of 552 calves from 27 dairy herds were collected. Cryptosporidium spp. oocysts were detected by light microscopy using Kinyoun staining. The sampled animals were clinically examined and farmers were interviewed about management procedure. Putative risk factors were tested for association using logistic regression. Oocyst shedding calves were found in 66.6% of herds and 16.3%of calves. Within-herd prevalence ranged from 0 to 60%, with a median of 8%.Cryptosporidium spp. excretion was not associated with the type of liquid diet, the animal gender, time the calf stayed with the dam after birth, the use of antibiotics and the presence of blood in feces. Risk factors of oocyst shedding in calves were young age, occurrence of diarrhea and spring-summer as calving season. The association with young age suggests an early exposure of neonatal calves to Cryptosporidiium spp. oocysts in maternity pens and\/or an increased age-related susceptibility. Association with diarrhea suggests that Cryptosporidium spp. is as a primary enteropathogen responsible for the cause of the observed diarrheal syndrome. The association with calving season may suggest that the typical weather conditions in spring-summer favor oocyst transmission. Results demonstrate that Cryptosporidium spp. infection is widespread in the study region. Monitoring and control of this parasitic protozoan infection in dairy herds is recommended.","palabras_clave":"CRYPTOSPORIDIUM|PREVALENCE|RISK FACTORS|CROSS-SECTIONAL STUDY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3945,"idioma_id":4,"titulo":"Whole plant open chamber to measure  gas exchange on herbaceous plants","resumen":"Whole plant open chamber to measure gas exchange on herbaceous plants. &nbsp;FERRARI, FLORENCIA NOEMI; CARLOS ALBERTO PARERA AND CARLOS BERNARDO PASSERA. Chilean Journal of Agricultural Research; (Print ISSN: 0718-5820; Online ISSN: 0718-5839) 76(1): 93-99. 2016.","palabras_clave":"Carbon dioxide| evapotranspiration|evaporation|photosynthesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3946,"idioma_id":4,"titulo":"Lynch syndrome in South America: past, present and future","resumen":"After decades of unawareness about Lynch syndrome, the medical community in South America is increasingly interested and informed. The visits and support of mentors like H. T. Lynch had been crucial to this awakening. Several countries have at least one registry with skilled personnel in genetic counseling and research. However, this only represents a very restricted resource for the region. According to the GETH, there are 27 hereditary cancer care centers in South America (21 in Brazil, 3 in Argentina, 1 in Uruguay, 1 in Chile and 1 in Peru). These registries differ in fundamental aspects of function, capabilities and funding, but are able to conduct high quality clinical, research and educational activities due to the dedication and personal effort of their members, and organizational support. More support from the governments as well as the participation of the community would boost the initiatives of people leading these groups. Meantime, the collaboration among the South American registries and the involvement of registries and leaders from developed countries will allow to maximize the efficiency in caring for affected patients and their families. The aim of this article is to describe how the knowledge of LS began to be spread in South America, how the first registries were organized and to summarize the current state of progress. In addition, we will provide an update of the clinical and molecular findings in the region.","palabras_clave":"LYNCH SYNDROME|HEREDITARY COLORECTAL CANCER|GENETIC COUNSELING|GENETIC TESTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3947,"idioma_id":4,"titulo":"Performance of stainless steel 316L electrodes with modified surface to be use in alkaline water electrolyzers","resumen":"In this experimental work the performance analysis of four different kinds of stainless steel 316L electrodes to be used in alkaline water electrolyzers for hydrogen production was carried out. The proposed electrodes were: electrodes in their original condition, electrodes with chemical pickling treatment, mechanized electrodes with straight-parallel topology and mechanized electrodes with straight-parallel topology and chemical pickling treatment. The study was repeated for two different spaces between electrodes: 5.3 and 6.1&nbsp;mm. All the electrodes presented a better performance in the longer distance because in the shorter one the effect of bubble resistance was more notorious. The mechanized electrodes with straight-parallel topology presented the best performance for an increase in the active area of the electrode by approximately 50%, which allowed to increase the current density by an average of 50%. For an equal amount of hydrogen produced between the best and the worst system, the heat released was higher by the system with lower efficiency.","palabras_clave":"Alkaline electrolyzers|Straight-parallel topology electrodes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3948,"idioma_id":4,"titulo":"Surviving nebulization-induced stress: dexamethasone in pH-sensitive  archaeosomes","resumen":"Aims. To increase the subcellular delivery of dexamethasone phosphate (DP) and stability to nebulization stress,  pH-sensitive  nanoliposomes  exhibiting  archaeolipids  acting  as  ligands  for  scavenger  receptors (pH-sensitive archaeosomes (ApH) were prepared. Materials  & methods.  The  anti-inflammatory  effect  of  0.18  mg  DP\/mg  total  lipid,  100-150  nm  DP containing  ApH  (dioleylphosphatidylethanolamine  (DOPE):  Halorubrum  tebenquichense total  polar archaeolipids (TPA): cholesterylhemisuccinate (CHEMS) 4.2:2.8:3 w:w) was tested on different cell lines. Size and HPTS retention of ApH and conventional pH-sensitive nanoliposomes  (LpH, DOPE:CHEMS 7:3 w:w) before and after nebulization were determined Results & conclusions.  DP-ApH  suppressed  IL-6  and  TNF-á on  phagocytic  cells.  Nebulized  after  6-month  storage,  LpH  increased  size  and   completely  lost  its  HPTS  while  ApH3  conserved  size  and polydispersity, fully retaining its original HPTS content","palabras_clave":"SUBCELLULAR DELIVERY|PH SENSITIVE|NEBULIZATION |MACROPHAGES TARGETING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3949,"idioma_id":4,"titulo":"Carrier Deformability in Drug Delivery","resumen":"Deformability is a key property of drug carriers used to increase the mass penetration across the skin without disrupting the lipid barrier. Highly deformable vesicles proved to be more effective than conventional liposomes in delivering drugs into and across the mammalian skin upon topical non occlusive application. In the past five years, highly deformable vesicles have been used for local delivery of drugs on joint diseases, skin cancer, atopic dermatitis, would healing, psoriasis, scar treatment, fungal, bacteria and protozoa infections. Promising topical vaccination strategies rely also in this type of carriers. Here we provide an overview on the main structural and mechanical features of deformable vesicles, to finish with an extensive update on their latest preclinical applications.","palabras_clave":"TOPICAL DRUG DELIVERY|HIGHLY DEFORMABLE VESICLES|SKIN CANCER|TOPICAL VACCINATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3950,"idioma_id":4,"titulo":"Ca(II) and Ce(III) homogeneous alginate hydrogels from parent alginic acid precursor: a structural study","resumen":"Alginate hydrogels are suitable for the encapsulation of biomolecules and microorganisms for the buildingof bioactive materials. Several alternatives to the conventional alginate formulation are being studied for abroad range of biotechnological applications; among them the crosslinking of alginate by lanthanidecations, Ln(III), envisages expanded biomedical applications. The performance of these functionalmaterials is highly related to the microstructure of the alginate matrix, which in turn is affected by theconditions of synthesis. In particular, when a diffusing gradient of the crosslinking cation is involved,microstructure inhomogeneities are expected at the macroscopic level. Here we discuss the subtle differencesin the microstructure, as assessed by SAXS (Small Angle X-ray Scattering), established in the directionof the gradient of diffusion of Ca(II) or Ce(III).","palabras_clave":"CALCIUM|CERIUM|ALGINATE|HOMOGENEOUS|LANTHANIDE|SAXS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3951,"idioma_id":4,"titulo":"The potential influence of atherogenic dyslipidemia on the severity of chronic Chagas heart disease","resumen":"Introduction: Chronic Chagas heart disease (CCHD) is the most common manifestation of American Trypanosomiasis, causing about 50,000 deaths annually. Several factors bear correlation with the severity of CCHD. However, to our knowledge, the assessment on the contribution of major cardiovascular risk factors (CRF), such as hypertension and atherogenic dyslipidemia (AD) to CCHD severity is scarce, despite their well-established role in coronary artery disease, heart failure and stroke.Objective: To explore the potential relationship of blood pressure and AD with the clinical profile of patients with CCHD.Methods: We performed a cross-sectional study in T. cruziseropositive patients categorized according to a standard CCHD classification. All individuals were subjected to complete clinical examination. Autoantibodies induced by T. cruzi were assessed by ELISA.Results: We observed that Atherogenic index (AI) levels rose significantly in relation to the severity of the CCHD stage, with CCHD III cases showing the highest values of AI. Furthermore, those patients with globally dilated cardiomyopathy with reduced ejection fraction showed higher levels of AI. In regard to autoantibodies, anti-B13 also showed relation with the severity of the disease.Conclusion: We observed that AI correlated with CCHD stages and contributed, in association with anti-B13 antibodies and age, to the prediction of systolic heart failure.","palabras_clave":"CHAGAS DISEASE|CHAGAS CARDIOMYOPATHY|DYSLIPIDEMIAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3952,"idioma_id":4,"titulo":"EXAMINING THE INFLUENCE OF METEOROLOGICAL SIMULATIONS FORCED BY DIFFERENT INITIAL AND BOUNDARY CONDITIONS IN VOLCANIC ASH DISPERSION MODELLING","resumen":"The performance of the combination of the FALL3D ash dispersion model with the Weather Research and Forecast(WRF) meteorological model in the southern cone of South America under two initial and boundary conditionswas evaluated. ERA-Interim and NCEP-GFS datasets were used as dynamic conditions by WRF to simulatemeteorological fields for FALL3D. As a study case, we used the eruption of the Puyehue?Cordón Caulle VolcanicComplex occurred in Chile in June 2011. The simulated meteorological results were compared with the horizontalwind direction, meridional and zonal wind components, air and dew point temperatures of 7 radio soundingstations using a set of error indicators. In addition, the ash mass load simulated by FALL3D for a day of maximumdispersion of volcanic ash was evaluated using the Moderate Resolution Imaging Spectroradiometer (MODIS)data, on which the Prata algorithm was applied. As well as this, the WRF-dominant physical processes withboth dynamic conditions were analyzed for that same date.Meteorological results indicated that the simulation performed with WRF and NCEP-GFS shows the lowest errorsat levels between 925 and 300 hPa. Ash dispersion simulated with FALL3D and WRF in both dynamic conditionsshows a different perfomance, which from the synoptic and dynamic viewpoint can be explained for the result ofwind intensity and geopotential height. Moreover, WRF intiliazed with NCEP-GFS and FALL3D has a higherdegree of concordance with the MODIS image. Based on the analysis and results, it was concluded that for thesouthern cone of South America, 1) it was not trivial for the simulation of volcanic ash dispersion to use one dynamiccondition or another in WRF; 2) in that sense, meteorological variables that influenced the differences involcanic ash dispersion were horizontal wind intensity and direction and geopotential heights; 3) the systemgenerated from the combination of the WRF model initialized with NCEP-GFS and the FALL3D dispersionmodel would provide better estimations of ash plume position and deposition in the region.","palabras_clave":"FALL3D|NCEP-GFS analysis|ERA interim|2011 PEyehue"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3953,"idioma_id":5,"titulo":"Trazabilidad y validación de requerimientos funcionales de sistemas informáticos mediante la transformación de modelos conceptuales","resumen":"El objetivo de este trabajo es caracterizar un método que permita latrazabilidad y validación de requerimientos funcionales de un sistema deinformación mediante la transformación de modelos conceptuales. Para lo cual seconstruyó un software denominado SIAR (Sistema Integral de Administración deRequerimientos) que administra los requerimientos funcionales y utiliza UML(Lenguaje Unificado de Modelado) para su representación como Casos de Uso. La finalidad principal de esta aplicación web es la gestiónde Casos de Uso con una herramienta que agilice su registración, normalice sucontenido y posibilite la trazabilidad de los cambios e implementarvalidaciones funcionales. Por ejemplo, un procedimiento automatizado deanálisis de consistencia de Casos de Uso, para lo cual el sistema genera ungrafo con la transición de estados de cada Caso de Uso que es analizado en unsimulador de autómata finito determinista para verificar la cohesión de losescenarios en él definidos.","palabras_clave":"Trazabilidad|Validación|Requerimiento funcional|Modelo conceptual"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3954,"idioma_id":4,"titulo":"Bioequivalence study of two long-acting formulations of oxytetracycline following intramuscular administration in bovines","resumen":"The aim of this study was to evaluate the bioequivalence of two commercial long-acting formulations based on oxytetracycline (OTC) hydrochloride between the reference formulation (Terramycin LA, Pfizer) and a test formulation (Cyamicin LA, Fort Dodge Saude Animal). Both formulations were administered in a single intramuscular route at a dose of 20 mg OTC\/kg of body weight in clinically healthy bovines. The study was carried out according to a one-period parallel design. Plasma samples were analyzed by high-pressure liquid chromatography. The limit of quantitation was 0.050 &#956;g\/mL with an accuracy of 101.67% with a coefficient of variation of 13.15%. Analysis of variance and 90% confidence interval tests were used to compare the bioavailability parameters (maximum plasma concentration, Cmax, and the area under the concentration-versus-time curve extrapolated to infinity, AUC0?&#8734;) of both products. In the case of the time to maximum concentration (Tmax), non-parametric tests based on Wilcoxon?s signed rank test were preferred. The comparison of the mean AUC0?&#8734; values did not reveal any significant differences (311.40 ± 93.05 &#956;g h\/mL and 287.71 ± 45.31 &#956;g h\/mL, respectively). The results were similar for the Tmax (3.58 ± 0.90 h versus 3.42 ± 0.51 h). However, when comparing the mean Cmax some significant differences were found (8.73 ± 3.66 &#956;g\/mL and 10.43 ± 3.84 &#956;g\/mL, respectively). The 90% confidence intervals for the ratio of AUC0?&#8734; and Tmax values for the reference and test product are within the interval 80?125%, but the 90% confidence intervals for the ratio of Cmax falls outside the proposed interval. It was concluded that Cmax of test product are not within the 20% of those of the reference, thus suggesting that test OTC is not bioequivalent to the reference formulation.","palabras_clave":"bioequivalence|oxytetracycline|long action|bovine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3955,"idioma_id":5,"titulo":"Desarrollo de una visualización relacional partiendo del glosario de Moodle","resumen":"El artículo presenta las bases teórico-metodológicas, junto a las características tecnológicas básicas, de una herramienta integrada de código abierto denominada Telares de la Memoria. La metodología implementada para la innovación se fundamentó en técnicas de la etnografía, de la investigación acción y de la ingeniería del software bajo el enfoque socio-técnico participativo. La herramienta ofrece el fichaje de datos hipervinculados con una original visualización, la carga de archivos con distintos formatos y la interoperabilidad con la red social Facebook. Uno de los aportes es que reutiliza el glosario de la plataforma educativa Moodle, posibilitando su instalación de manera sencilla en dicho entorno. Como origen del desarrollo, se hace referencia a un dispositivo socio-técnico interdisciplinario y participativo que tuvo por objetivo abordar la escritura colaborativa de la memoria plural en una pequeña población de Argentina. Esto involucra, en los aspectos sociales y específicamente educativos, metodologías que articulan técnicas, tecnologías y propuestas con dinámicas colaborativas con una utilización extensiva y abierta de artefactos digitales aptos para múltiples propósitos pedagógicos. La usabilidad de Telares de la Memoria resultó satisfactoria, y puede ser reutilizada en distintos niveles y modalidades educativas efectuando simples adecuaciones ya que posibilita poner en obra un abordaje sistémico complejo para el tratamiento de los contenidos. En este sentido, puede favorecer la emergencia de nuevos procesos relacionales que soliciten una construcción interdisciplinaria del conocimiento.","palabras_clave":"MOODLE|GLOSARIO|DISPOSITIVO HIPERMEDIAL DINAMICO|TELARES DE LA MEMORIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3956,"idioma_id":4,"titulo":"Paclitaxel-loaded Polymersomes for Enhanced Intraperitoneal Chemotherapy","resumen":"Peritoneal carcinomatosis is present in more than 60% of gastric cancer, 40% of ovarian cancer, and 35% of colon cancer patients. It is the second most common cause of cancer mortality, with a median survival of 1-3 months. Cytoreductive surgery combined with intraperitoneal chemotherapy is the current clinical treatment, but achieving curative drug accumulation and penetration in peritoneal carcinomatosis lesions remains an unresolved challenge. Here we employed flexible and pH-sensitive polymersomes for payload delivery to peritoneal gastric (MKN-45P) and colon (CT26) carcinoma in mice. Polymersomes were loaded with Paclitaxel® and in vitro drug release was studied as a function of pH and time. Paclitaxel-loaded polymersomes remained stable in aqueous solution at neutral pH for up to four months. In cell viability assay on cultured cancer cell lines (MKN-45P, SKOV3, CT26), Paclitaxel-loaded polymersomes were more toxic than free drug or albumin-bound Paclitaxel (Abraxane®). Intraperitoneally administered fluorescent polymersomes accumulated in malignant lesions, and immunofluorescence revealed intense signal inside tumors with no detectable signal in control organs. A dual targeting of tumors was observed: direct (circulation independent) penetration, and systemic, blood vessel-associated accumulation. Finally, we evaluated preclinical antitumor efficacy of polymersomes-paclitaxel in treatment of MKN-45P disseminated gastric carcinoma using a total dose of 7 mg\/kg. Experimental therapy with polymersome-Paclitaxel improved the therapeutic index of drug over Paclitaxel-Cremophor and Abraxane®, as evaluated by intraperitoneal tumor burden and number of metastatic nodules. Our findings underline the potential utility of the polymersome platform for delivery of drugs and imaging agents to peritoneal carcinomatosis lesions.","palabras_clave":"Polymersomes|Peritoneal carcinomatosi|Paclitaxel |Gastric cancer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3957,"idioma_id":4,"titulo":"Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae","resumen":"We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatusmidgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day, continuous starvation until 80 day, and continuous starvation throughout 50 day and then feeding for the following 30 days. Juveniles from each protocol were weighed, and sacrificed at day 15, 30, 50 or 80. The lipids, glycogen, reduced glutathione (GSH), soluble protein, lipid peroxidation (TBARS), protein oxidation (PO), catalase (CAT), lipase and proteinase activities, and histology were measured on midgut gland. Starved crayfish had a lower hepatosomatic index, number of molts, specific growth rate, lipids, glycogen,and GSH levels than fed animals at all assay times. The starvation did not affect the soluble protein, TBARS, PO levels and CAT. In starved juveniles the lipase activity decreased as starvation time increased, whereas proteinase activity decreased only at day 80. The histological analysis of the starved animals showed several signs of structural alterations. After 30 days of feeding, the starved-feeding animals exhibited a striking recovery of hepatosomatic index, number of molts, lipids and glycogen, GSH, lipase activity and midgut gland structure.","palabras_clave":"Starvation|Physiology|Midgut gland|Cherax quadricarinatus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3959,"idioma_id":4,"titulo":"High quality residues from cover crops favor changes in microbial community and enhance C and N sequestration","resumen":"The objective of the study was to evaluate the effect of a change in management on the soil microbial community and C sequestration. We conducted a 3-year field study in La Pampa (Argentina) with rotation of sorghum (Sorghum bicolor) in zero tillage alternating with rye (Secale cereale) and vetch (Vicia villosa ssp. dasycarpa). Soil was sampled once a year at two depths. Soil organic matter fractions, dissolved organic matter, microbial biomass (MBC) and community composition (DNA extraction, qPCR, and phospholipid FAME profiles) were determined. Litter, aerial- and root biomass were collected and all material was analyzed for C and N. Results showed a rapid response of microbial biomass to a bacterial dominance independent of residue quality. Vetch had the highest diversity index, while the fertilized treatment had the lowest one. Vetch?sorghum rotation with high N mineralization rates and diverse microbial community sequestered more C and N in stable soil organic matter fractions than no-till sorghum alone or with rye, which had lower N turnover rates. These results reaffirm the importance of enhanced soil biodiversity for maintaining soil ecosystem functioning and services. The supply of high amounts of N-rich residues as provided by grass?legume cover crops could fulfill this objective.","palabras_clave":"VETCH|COVER CROP|DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3960,"idioma_id":4,"titulo":"Effect of different cover crops on C and N cycling in sorghum NT systems","resumen":"In many no-till (NT) systems, residue input is low and fallow periods excessive, for which reasons soil degradation occurs. Cover crops could improve organic matter, biological activity, and soil structure. In order to study changes in soil carbon, nitrogen and microbial biomass a field experiment (2010-2012) was set up with sorghum (Sorghum bicolor Moench.) monoculture and with cover crops. Treatments were control (NT with bare fallow), rye (Secale cereale L.) (R), rye with nitrogen fertilization (R+N), vetch (Vicia villosa Roth.) (V), and rye-vetch mixture (VR) cover crops. A completely randomized block design with 4 replicates was used. Soil was sampled once a year at 0.06 and 0.12 m depth for total C, microbial biomass carbon (MBC) and-nitrogen (MBN) determinations. Shoot and root biomass of sorghum and cover crops, litter biomass, and their respective carbon and nitrogen contents were determined. Soil temperatures at 0.06 and 0.12 m depth, volumetric water contents and nitrate concentrations were determined at sowing, and harvest of each crop, and during sorghum?s vegetative phase. NT led to a small increase in MBC and MBN, despite low litter and root biomass residue. Cover crops increased litter, root biomass, total C, MBC, and MBN. Relationships between MBC, MBN, and root-C and ?N adjusted to logistic models (R2 = 0.61 and 0.43 for C and N respectively). Litter cover improved soil moisture to 45-50% water filled pore space and soil temperatures not exceeding 25 °C during the warmest month. Microbial biomass stabilized at 20.1 g C m-2 and 1.9 g N m-2 in the upper 0.06 m.  Soil litter disappearance was a good indicator of mineral N availability. These findings support the view that cover crops, specifically legumes in NT systems can increase soil ecosystem services related to water and carbon storage, habitat for biodiversity, and nutrient availability.","palabras_clave":"Soil environment|Microbial biomass|Element cycling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3961,"idioma_id":4,"titulo":"Increase in the quality of the prediction of a computational wildfire behavior method through the improvement of the internal metaheuristic","resumen":"Wildfires cause great losses and harms every year, some of which are often irreparable. Among the different strategies and technologies available to mitigate the effects of fire, wildfire behavior prediction may be a promising strategy. This approach allows for the identification of areas at greatest risk of being burned, thereby permitting to make decisions which in turn will help to reduce losses and damages. In this work we present an Evolutionary-Statistical System with Island Model, a new approach of the uncertainty reduction method Evolutionary-Statistical System. The operation of ESS is based on statistical analysis, parallel computing and Parallel Evolutionary Algorithms (PEA). ESS-IM empowers and broadens the search process and space by incorporating the Island Model in the metaheuristic stage (PEA), which increases the level of parallelism and, in fact, it permits to improve the quality of predictions.","palabras_clave":"WILDFIRE BEHAVIOR PREDICTION|SIMULATION|UNCERTAINTY REDUCTION|PARALLEL EVOLUTIONARY ALGORITHMS|STATISTICAL SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3962,"idioma_id":4,"titulo":"CVD Graphene Transferred with Au Nanoparticles: An Ideal Platform for TERS and SERS on a Single Triangular Nanoplate","resumen":"Transferring CVD graphene using surfactantprotected Au nanoparticles (Au NPs) avoids the use of PMMA and opens new ways of building heterojunctions for TERS and SERS applications. Thermal treatment removes organics from&nbsp; the Au NPs and leads to larger nanoparticles and theformation of networks, dimmers, and nanoplates of various shapes (circles, hexagons, and triangles). Raman and HRTEM revealed bilayer graphene and Moire&#769;patterns as determined bythe 2D band and dislocated atomic layers, respectively. TERS performed on the heterojunction exhibits a reversible increase in frequency and sharpening of the characteristic bands in graphene along with the exaltation of bands such as D + D&#8242;. A close examination into a single&nbsp; Triangular-shape nanoplate reveals that the most irregular area of the nanoplate is consistent with the higher Raman enhancement of Rh6G when compared to other areas of the same feature. This work may shed light onto new ways of transferring graphene, fundamental aspects concerning local strains in the heterojunction, and SERS on a single Au nanostructure.","palabras_clave":"CVD graphene|Au nanoparticles|SERS|TERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3963,"idioma_id":4,"titulo":"Photothermal triggering of self-healing processes applied to the reparation of bio-based polymer networks","resumen":"Green laser irradiation successfullyactivated self-healing processes in epoxy-acid networks modified with lowamounts of gold nanoparticles (NPs). A bio-based polymer matrix, obtained bycrosslinking epoxidised soybean oil (ESO) with an aqueous citric acid (CA)solution, was self-healed through molecular rearrangements produced bytransesterification reactions of &#946;-hydroxyester groups generated in thepolymerization reaction. The temperature increase required for the triggeringof these thermally-activated reactions was attained by green light irradiationof the damaged area. Compression force needed to assure a good contact betweencrack faces was achieved by volume dilatation generated by the same temperaturerise. Gold NPs dispersed in the polymer efficiently generated heat in thepresence of electromagnetic radiation under plasmon resonance, acting asnanometric heating sources and allowing remote activation of the self-healing inthe crosslinked polymer. ","palabras_clave":"polymeric nanocomposite|photothermal effect|self-healing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3964,"idioma_id":4,"titulo":"Trypanocidal and leishmanicidal activities of flavonoids isolated from Stevia satureiifolia var. satureiifolia","resumen":"Chagas? disease and leishmaniasis produce significant disability and mortality with great social and economic impact. The genus Stevia (Asteraceae) is a potential source of antiprotozoal compounds.Objective Aerial parts of four Stevia species were screened on Trypanosoma cruzi. Stevia satureiifolia (Lam.) Sch. Bip. var. satureiifolia (Asteraceae) dichloromethane extract was selected for abioassay-guided fractionation in order to isolate its active compounds. Additionally, the antileishmanial activity and the cytotoxicity of these compounds on mammalian cells were assessed.The dichloromethane extract was fractionated by column chromatography.The isolated compounds were evaluated using concentrations of 0?100 mg\/mL on T. cruzi epimastigotes and on Leishmania braziliensis promastigotes for 72 h, on trypomastigotes and amastigotes of T. cruzi for 24 h and 120 h, respectively. The compounds? cytotoxicity (12.5?500 ug\/mL) was assessed on Vero cells by the MTT assay. The structure elucidation of each compound was performed by spectroscopic methods and HPLC analysis.Results The dichloromethane extracts of Stevia species showed significant activity on T. cruzi epimastigotes. The flavonoids eupatorin (1.3%), cirsimaritin (1.9%) and 5-desmethylsinensetin (1.5%) were isolated from S. satureiifolia var. satureiifolia extract. Eupatorin and 5-desmethylsinensetin showed IC50 values of 0.2 and 0.4 ug\/mL on T. cruzi epimastigotes and 61.8 and 75.1 ug\/mL on trypomastigotes, respectively. The flavonoid 5-desmethylsinensetin showed moderate activity against T. cruzi amastigotes (IC50 value=78.7 ug\/mL) and was the most activecompound on L. braziliensis promastigotes (IC50 value=37.0 ug\/mL). Neither of the flavonoids showed cytotoxicity on Vero cells, up to a concentration of 500 ug\/mL.","palabras_clave":"Trypanosoma cruzi|Leishmania braziliensis|Stevia spp|phenolic compounds"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3965,"idioma_id":4,"titulo":"A Comparative Study of New Aspergillus Strains for Proteolytic Enzymes Production by Solid State Fermentation","resumen":"A comparative study of the proteolytic enzymes production using twelve Aspergillus strains previously unused for this purpose was performed by solid state fermentation. A semiquantitative and quantitative evaluation of proteolytic activity were carried out using crude enzymatic extracts obtained from the fermentation cultures, finding seven strains with high and intermediate level of protease activity. Biochemical, thermodynamics, and kinetics features such as optimum pH and temperature values, thermal stability, activation energy (E a), quotient energy (Q 10), K m , and V max were studied in four enzymatic extracts from the selected strains that showed the highest productivity. Additionally, these strains were evaluated by zymogram analysis obtaining protease profiles with a wide range of molecular weight for each sample. From these four strains with the highest productivity, the proteolytic extract of A. sojae ATCC 20235 was shown to be an appropriate biocatalyst for hydrolysis of casein and gelatin substrates, increasing its antioxidant activities in 35% and 125%, respectively.","palabras_clave":"Aspergillus |Protease|Solid State Fermentation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3966,"idioma_id":5,"titulo":"Dimensiones políticas de una epidemia: el caso de la gripe A (H1N1) en la prensa escrita de Argentina","resumen":"O presente estudo tem como objetivo compreender as representações sociais sobre a epidemia de Influenza A H1N1 na Argentina em 2009, nos jornais de maior circulação no país. A metodologia foi qualitativa e quantitativa a partir da análise de duas dimensões: a forma em que se constrói o ?objeto? epidemia, (designação e caracterização dos mesmos), e as fontes de informação das notícias, procurando identificar em cada caso os atores sociais envolvidos.Os resultados mostram que na nomeação da epidemia fica a cerca de decisão política de eliminar a responsabilidade de um modo de produção de gado de risco, culpando o indivíduo. Observa-se que as recomendações dos representantes políticos, especialmente a nível internacional e promovida a farmacologización da epidemia, a reprodução do posicionamento da população em suas reivindicações que demonstra a validade da hegemonia biomédica e, finalmente, o comportamento da prensa para a epidemia concentra-se em puramente ligada aos acontecimentos políticos locais.","palabras_clave":"Medios de Comunicación, |Periódicos| Virus de la Influenza A Subtipo H1N1 |Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3967,"idioma_id":4,"titulo":"Light-responsive hybrid material based on luminescent core-shell quantum dots and steroidal organogel","resumen":"We report the synthesis of a smart novel hybrid with reversible photoswitchable luminescence properties modulated by light. The combination of a low molecular weight organogelator (LMOG) and CdSe\/ZnS core-shell semiconductor nanoparticles capped with trioctylphosphine oxide ligands produces a luminescent, stable and transparent material. Modulation of the luminescence properties was successfully achieved using a diarylethene photochromic compound, with good resistance to fatigue ca. 22 cycles. Interestingly, the morphology of the organogel fibers was preserved in the hybrid, while a partial luminescence quenching of the nanoparticle was observed. This material could have implication for on-and-off photoswitching applications.","palabras_clave":"ORGANOGEL|QUANTUM DOTS|HYBRID GEL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3968,"idioma_id":4,"titulo":"Topotecan Delivery to the Optic Nerve after Ophthalmic Artery Chemosurgery.","resumen":"Extraocular retinoblastoma is a major challenge worldwide, especially in developing countries. Current treatment involves the administration of systemic chemotherapy combined with radiation, but there is a clear need for improvement of chemotherapy bioavailability in the optic nerve. Our aim was to study the ophthalmic artery chemosurgery (OAC) local route for drug delivery assessing ocular and optic nerve exposure to chemotherapy and to compare it to exposure after intravenous infusion (IV) of the same dose in an animal model. Topotecan was used as a prototype drug that is active in retinoblastoma and based on the extensive knowledge of its pharmacokinetics in preclinical and clinical settings. Five Landrace pigs received 4mg of topotecan via OAC as performed in retinoblastoma patients. At the end of the infusion, the eyes were enucleated, the optic nerve and retina were dissected, and the vitreous and plasma were separated. After recovery and a wash-out period, the animals received a 30-min IV infusion of topotecan (4 mg). The remaining eye was enucleated and tissues and fluids were separated. All samples were stored until quantitation using HPLC. A significantly higher concentration of topotecan in the optic nerve, vitreous, and retina was obtained in eyes after OAC compared to IV infusion (p0.05). The median optic nerve-to-plasma ratio after OAC and IV was 44 and 0.35, respectively. Topotecan OAC delivery attained an 80-fold higher concentration in the optic nerve compared to the systemic infusion of the same dose with similar plasma concentrations in a swine model. Patients with retinoblastoma extension into the optic nerve may benefit from OAC for tumor burden by increased chemotherapy bioavailability in the optic nerve without increasing systemic exposure or toxicity.","palabras_clave":"Retinoblastoma|Topotecan"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3969,"idioma_id":4,"titulo":"Pharmacokinetics of Melphalan After Intravitreal Injection in a Rabbit Model.","resumen":"PURPOSE:Although widely used for vitreous seed control in retinoblastoma patients, currently there are no data on melphalan pharmacokinetics after intravitreal injections. Therefore, in this study, we characterized the ocular and systemic disposition of melphalan after intravitreal injection in the rabbit eye.METHODS:New Zealand rabbits received a single intravitreal injection of 15&#8201;&#956;g of melphalan. Vitreous, aqueous, retina, and blood samples were collected at different times up to 12&#8201;h after the injection. Melphalan was quantitated in the biological samples using a validated high-performance liquid-chromatography technique and pharmacokinetic parameters were calculated by means of compartmental models.RESULTS:Model-predicted melphalan maximum vitreous, aqueous, and retina concentrations were 7.8&#8201;&#956;g\/mL, 0.024&#8201;&#956;g\/mL, and 9.8&#8201;&#956;g\/g tissue, respectively, attained immediately and at 0.8 and 0.25&#8201;h after intravitreal injection. Melphalan vitreous concentrations were higher than 0.3&#8201;&#956;g\/mL for 5&#8201;h after dosing. The elimination half-life from the vitreous, aqueous humor, and retina was 1.0, 0.2, and 1.2&#8201;h, respectively. Aqueous exposure [area under the curve (AUC)] was only 0.7% of that of the vitreous AUC. Melphalan concentrations in the retina were still detectable 12&#8201;h after dosing, while plasma exposure was under the limit of quantitation.CONCLUSION:Intravitreal administration of 15&#8201;&#956;g melphalan leads to pharmacological vitreous levels with low aqueous exposure. Melphalan concentrations in the retina were measurable up to 12&#8201;h after dosing, but we report nondetectable systemic exposure in the rabbit. The results correlate with the clinical features of retinoblastoma patients that show control of vitreous seeds without systemic toxicity using intravitreal melphalan.","palabras_clave":"MELPHALAN|PHARMACOKINETICS|INTRAVITREAL|RABBIT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3970,"idioma_id":4,"titulo":"WHEAT BREAD ENRICHED WITH ORGANIC CALCIUM SALTS AND INULIN. A BREAD QUALITY STUDY","resumen":"The objective of this work was to study qualityparameters of enriched wheat bread with calcium citrate(Ca3CI2) or lactate (CaLA2) and inulin (In), also to optimizebread formulation. Fermentation time (tf), specific volume(Vs), browning index of crust (BI) and crumb properties(moisture, alveolus, texture) were studied. Generally, tf andVs decreased with prebiotic increment. Ca3CI2 did not changeVs at equal inulin quantity, whereas with CaLA2 smallerbreads were obtained (at 6.5 % In). Moisture of crumbs decreasedwith an increase in Ca3CI2 (at &#8804; 6.5 %); while forCaLA2 was more influenced by the prebiotic. Up to 6.5 %In, the addition of both salts decreased crumb firmness andincreased cohesiveness. Using a desirability function, the optimumcalcium-prebiotic bread obtained with Ca3CI2contained 2.40 g\/kg Ca and 7.49 % In and with CaLA2 presented1.33 g\/kg Ca and 4.68 % In. Breads of high-qualitywith higher calcium and prebiotic quantity were able to obtainwith Ca3CI2.","palabras_clave":"CALCIUM CITRATE|CALCIUM LACTATE|INULIN|WHEAT BREAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3971,"idioma_id":5,"titulo":"Variabilidad química del ?tomillo silvestre? (Acantholippia seriphioides, Verbenaceae) en la meseta Patagónica","resumen":"El tomillo silvestre es una planta aromática y medicinal de amplio uso en varias regiones de Argentina como medicinal ysaborizante. Se estudiaron poblaciones de Acantholippia seriphioides en dos zonas de la meseta chubutense, los parajes El Pajarito y LaRueda. Las partes aéreas oreadas fueron extraídas por hidrodestilación y los aceites esenciales se analizaron por GC-FID-MS. Losrendimientos en promedio de aceites esenciales fueron de 5.2 ml\/kg y 3.2 ml\/kg, para La Rueda y El Pajarito respectivamente. Los tiposquímicos encontrados fueron: alfa-terpineno\/timol\/carvacrol en La Rueda y mirtenol\/geraniol\/carvona en El Pajarito. El probable quimiotipo deeste último sitio difiere marcadamente de los reportados en trabajos previos para esta especie y presenta además importante variabilidadcuantitativa, según el estado fenológico.","palabras_clave":"Acantholippia seriphioides|Essential oils|Chemotypes|Patagonia Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3972,"idioma_id":4,"titulo":"Following human pointing: Where do dogs (Canis familiaris) look at to find food?","resumen":"Domestic dogs (Canis familiaris) are notably skillful in following cues from people (e.g., pointing gestures). However, not much is known about the processing of information available during such tasks. We here focus on one of the earliest of such processes, namely attention. The goal of the present work was to describe variations in dogs? attention towards diverse targets while they solve an object choice task with human pointing. The direction of subjects? gaze was measured in the period comprising one second before and two seconds after the experimenter called the dog and simultaneously performed a static distal pointing gesture towards the correct bowl. We did two consecutive training phases: acquisition and extinction. Dogs spent more time watching the pointer than the pointing gesture itself and the correct than the incorrect bowl. Indeed, the time spent watching the correct bowl was the best predictor of correct choices across phases. We discuss the relevance of these findings for the process of local enhancement.","palabras_clave":"ATTENTION|DOGS|GAZE DIRECTION|OBJECT CHOICE TASK |POINTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3973,"idioma_id":4,"titulo":"Hyaluronan oligomers sensitize chronic myeloid leukemia cell lines to the effect of Imatinib","resumen":"Chronic myeloid leukemia (CML) is a myeloproliferative syndrome characterized by the presence of the Philadelphia chromosome. As a consequence, a fusion gene (bcr-abl) encoding a constitutively active tyrosine kinase is generated. The first line treatment consists on BCR-ABL inhibitors such as Imatinib. Nevertheless, such treatment may lead to the selection of resistant cells. Therefore, it is of great value to find molecules that enhance the anti-proliferative effect of first-line drug. Hyaluronan is the main glycosaminglican of the extracellular matrix and it is involved in tumor progression and multidrug resistance. 4-Methylumbelliferone (4MU) is the inhibitor of hyaluronan synthesis and it is known that this drug is able to abrogate the proliferation of several carcinoma cells. We have previously demonstrated that 4MU induces senescence and can revert Vincristine resistance in human leukemia cell lines. However, little is known about the role of 4MU in hematological malignancies. The aim of this work was to determine whether the combination of 4MU with Imatinib combination is able to modulate the proliferation as well as apoptosis and senescence induction in human CML cell lines (K562 and Kv562). We demonstrated that 4MU and Imatinib co-treatment abrogated the proliferation of both cell lines increasing senescence and keeping the levels of apoptosis induced by Imatinib. These findings highlight the potential of 4MU for CML therapy.","palabras_clave":"Hyaluronan oligomers|Chronic myeloid leukemia |Imatinib"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3974,"idioma_id":4,"titulo":"Evidence of a pro-apoptotic effect of specific antibodies in a bovine macrophage model of infection with Mycobacterium avium subsp. paratuberculosis","resumen":"Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne?s disease (JD), achronic granulomatous enteritis in ruminants. Understanding the protective immune response followinginfection is crucial to improve the diagnosis and the development of vaccines against this disease. The goalof this work was to assess whether specific antibodies were able to modulate the macrophage responseto MAP infection by evaluating apoptosis and TNF- secretion in an in vitro model. Sera from healthy(n = 2), MAP-infected (n = 3) and lipoarabinomannan (LAM)-immunized (n = 3) bovines were evaluated.LAM was chosen as immunogen due to its relevant role in mycobacterial pathogenesis. We demonstratedby two different techniques (Acridine Orange\/Ethidium Bromide microscopy and Annexin V\/7-Amino-Actinomycin D flow cytometry) that the immune sera from both, MAP-infected and LAM-immunizedbovines, significantly increased macrophage apoptosis in infected cultures. Comparable levels of apoptosiswere detected when MAP was pre-incubated with purified specific antibodies instead of wholeserum. Furthermore, this effect was accompanied by a significantly higher secretion of TNF-. Theseresults strongly suggest that specific antibodies could limit the impact of MAP on the apoptosis of bovinecells. This work would contribute to elucidate the role of the specific antibody response in bovine JD andits prevention.","palabras_clave":"Mycobacterium avium subsp. Paratuberculosis|Apoptosis|Bovine macrophages"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3975,"idioma_id":4,"titulo":"Genetic characterisation of PPARG, CEBPA and RXRA, and their influence on meat quality traits in cattle","resumen":"BackgroundPeroxisome proliferator-activated receptor gamma (PPARG), CCAAT\/enhancer bindingprotein alpha (CEBPA) and retinoid X receptor alpha (RXRA) are nuclear transcription factors that play important roles in regulation of adipogenesis and fat deposition. The objectives of this study were to characterise the variability of these three candidate genes in a mixed sample panel composed of several cattle breeds with different meat quality, validate single nucleotide polymorphisms (SNPs) in a local crossbred population (Angus - Hereford - Limousin) and evaluate their effects on meat quality traits (backfat thickness, intramuscular fat content and fatty acid composition), supporting the association tests with bioinformatic predictive studies.ResultsGlobally, nine SNPs were detected in the PPARG and CEBPA genes within our mixedpanel, including a novel SNP in the latter. Three of these nine, along with seven other SNPs selected from the Single Nucleotide Polymorphism database (SNPdb), including SNPs in the RXRA gene, were validated in the crossbred population (N = 260). After validation, five of these SNPs were evaluated for genotype effects on fatty acid content and composition. Significant effects were observed on backfat thickness and different fatty acid contents (P < 0.05). Some of these SNPs caused slight differences in mRNA structure stability and\/or putative binding sites for proteins.ConclusionsPPARG and CEBPA showed low to moderate variability in our sample panel.Variations in these genes, along with RXRA, may explain part of the genetic variation in fat content and composition. Our results may contribute to knowledge about genetic variation in meat quality traits in cattle and should be evaluated in larger independent populations.","palabras_clave":"POLYMORPHISM|VARIATION|ASSOCIATION|COWS|BEEF|SNP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3976,"idioma_id":4,"titulo":"Inhibition of the Formation of the Spf1p Phosphoenzyme by Ca2+","resumen":"AbstractP5-ATPases are important for processes associated with the endosomal-lysosomal system of eukaryotic cells. In humans, the loss of function of P5-ATPases causes neurodegeneration. In the yeastSaccharomyces cerevisiae, deletion of P5-ATPase Spf1p gives rise to endoplasmic reticulum stress. The reaction cycle of P5-ATPases is poorly characterized. Here, we showed that the formation of the Spf1p catalytic phosphoenzyme was fast in a reaction medium containing ATP, Mg(2+), and EGTA. Low concentrations of Ca(2+)in the phosphorylation medium decreased the rate of phosphorylation and the maximal level of phosphoenzyme. Neither Mn(2+)nor Mg(2+)had an inhibitory effect on the formation of the phosphoenzyme similar to that of Ca(2+) TheKmfor ATP in the phosphorylation reaction was &#8764;1 &#956;mand did not significantly change in the presence of Ca(2+) Half-maximal phosphorylation was attained at 8 &#956;mMg(2+), but higher concentrations partially protected from Ca(2+)inhibition. In conditions similar to those used for phosphorylation, Ca(2+)had a small effect accelerating dephosphorylation and minimally affected ATPase activity, suggesting that the formation of the phosphoenzyme was not the limiting step of the ATP hydrolytic cycle. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.","palabras_clave":"p5-ATPase|Calcium modulation|protein purification |protein phosphorylation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3977,"idioma_id":4,"titulo":"S-layer production by Lactobacillus acidophilus IBB 801 under environmental stress conditions","resumen":"The ability of microorganisms to synthesize S-layer, the outermost structure of the microbial cell envelope composed of non-covalently bound proteins, has been ascribed to help microorganisms to exert their probiotic properties in the host. In this work, formation of S-layer by the potentially probiotic strain Lactobacillus acidophilus IBB 801 under different stress culture conditions (high incubation temperatures, presence of bile salts or NaCl, and acidic pH) was assayed. A marked S-layer synthesis by L. acidophilus IBB 801 was detected when the strain was grown at 42 °C and in the presence of 0.05 % bile salts or 2.0 % NaCl. The presence of S-layer proteins was further confirmed by transmission electron microscopy and protein identification by MS\/MS. The differential expression of the proteome of this strain at 42 °C, when a marked formation of S-layer was detected, revealed the overexpression of six proteins mainly related to general stress and protein biosynthesis and translation, while four proteins detected in lower amounts were involved in DNA repair and energy metabolism. As L. acidophilus IBB 801 produces both a bacteriocin and S-layer proteins, the strain could be of interest to be used in the formulation of functional food products with specific properties","palabras_clave":"S-LAYER PROTEINS|LACTIC ACID BACTERIA|STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3978,"idioma_id":4,"titulo":"Mitogenic signaling pathways in the liver of growth hormone (GH)-overexpressing mice during the growth period","resumen":"ABSTRACT: Growth hormone (GH) is a pleiotropic hormone that triggers STATs, ERK1\/2 and Akt signaling, related to cell growth and proliferation. Transgenic mice overexpressing GH present increased body size, with a disproportionate liver enlargement due to hypertrophy and hyperplasia of the hepatocytes. We had described enhanced mitogenic signaling in liver of young adult transgenic mice. We now evaluate the activation of these signaling cascades during the growth period and relate them to the morphological alterations found. Signaling mediators, cell cycle regulators and transcription factors involved in cellular growth in the liver of GH-overexpressing growing mice were assessed by immunoblotting, RT-qPCR and immunohistochemistry. Hepatocyte enlargement can be seen as early as 2-weeks of age in GH-overexpressing animals, although it is more pronounced in young adults. Levels of cell cycle mediators PCNA and cyclin D1, and transcription factor c-Jun increase with age in transgenic mice with no changes in normal mice, whereas c-Myc levels are higher in 2-week-old transgenic animals and cyclin E levels decline with age for both genotypes. STAT3, Akt and GSK3 present higher activation in the adult transgenic mice than in the growing animals, while for c-Src and mTOR, phosphorylation in GH-overexpressing mice is higher than in control siblings at 4 and 9&nbsp;weeks of age. No significant changes are observed for ERK1\/2, neither by age or genotype. Thus, the majority of the mitogenic signaling pathways are gradually up-regulated in the liver of GH-transgenic mice, giving rise to the hepatic morphological changes these mice exhibit.","palabras_clave":"growth hormone|proliferation|growth|transgenic mice"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3979,"idioma_id":4,"titulo":"A comparative study of several microporous materials to store methane by adsorption","resumen":"Methane adsorption was studied in microporous materials with differences in their pore geometry and surface chemistry in order to evaluate the characteristics that enhance methane storage in porous materials. The studied materials included metal-organic frameworks (Cu-BTC, MIL-53 and ZIF-8), zeolites (5A and 13X) and an activated carbon (Maxsorb). Textural properties such as pore volume and specific surface area were studied by nitrogen adsorption?desorption isotherms. Methane adsorption was analyzed by means of measurements at low and high pressures (0?5000 kPa) at various temperatures (278?323 K). The effect of the adsorbate?adsorbent interaction was evaluated by the determination of the Henry constant using the adsorption data of methane at low pressures and 298 K. In addition, the isosteric enthalpy of adsorption using data of adsorption at several temperatures was obtained. A linear trend was observed between the Henry constant, the enthalpy of adsorption and the pore size, indicating that the latter is the most influential factor in the interaction of a microporous adsorbent and methane at room temperature. Also, it was found that the textural properties (i.e. specific surface and micropore volume) are directly related with the methane storage capacity at 298 K and 3.5 MPa. However, we obtained a deviation of this behavior for ZIF-8 that is related to its geometry and pore size.","palabras_clave":"Methane|Adsorption|Microporous"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3980,"idioma_id":4,"titulo":"Evaluation and Improvement of an Organizational Resource applying Strategy Patterns","resumen":"For any software company that frequently performs quality assurance activities devoted to mea-surement, evaluation (ME) and change\/improvement (MEC) projects, ME and MEC strategies can be valuable organizational assets. In this paper, we analyze the improvement of a ME strategy, which can be considered an organizational resource to be applied to quality assurance activities. This resource is called the GOCAME (Goal-Oriented Context-Aware Measurement and Evaluation) strategy. A ME\/MEC strategy embraces the next three integrated capabilities: i) the ME\/MEC domain conceptual base and framework; ii) the process perspective specifications; and, iii) the method specifications. The improvement of GOCAME was performed instantiating two strategy patterns. A strategy pattern is a reusable solution to recurrent problems in ME\/MEC projects. For an im-provement goal, the selected MEC strategy pattern allows instantiating in a project a set of tailored activities and methods for measurement, evaluation, analysis and change. Particularly, we instan-tiate the GoME_1QV (Goal-oriented Measurement and Evaluation for One Quality View) strategy pattern to understand the GOCAME current quality state and compare it with the so-called GQM+Strategies. First, this evaluation and analysis allows us to know the GOCAME strengths and weaknesses with regard to the quality of the three capabilities. Second, we instantiate the Go-MEC_1QV (Goal-oriented Measurement, Evaluation and Change for One Quality View) strategy pat-tern to improve the GOCAME current state, producing as result a new version of the GOCAME strategy.","palabras_clave":"Strategy Pattern|Quality View|Evaluation|Change|Improvement"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3981,"idioma_id":4,"titulo":"Delineation of site-specific management units for operational applications using the Topographic Position Index in La Pampa, Argentina","resumen":"In this work we propose the use of the topographic position index (TPI), which takes into account the local topography for a given neighborhood, to delineate management units (MU) for site-specific systems. This study was performed in the province of La Pampa, in central Argentina, an area with sandy soils where the main limiting condition for crops is soil moisture. Usually, multi-annual yield maps are used for the delineation of MU. However, those are strongly influenced by issues that could be related to un-calibrated data and previous agronomical practices. Thus, there was a need for a methodology based on stable and unbiased parameters. The methodology was developed for a representative 100 ha field. The average size and orientation of the topographic structures were characterized applying the autocorrelation function on the topographic data, which was then used to determine an optimum neighborhood size for the TPI. TPI performed better than the topographic map to characterize the variability of the field. The correlation between yield and TPI was higher (r = 0.74) than that between yield and topography (r = 0.54). The resulting management units were delineated using an unsupervised classification approach on the TPI maps. From the confusion matrices, the overall accuracy was higher for the TPI derived maps than for the topography derived maps (62% against 47%) when compared to a yield map used as reference. We estimate that this methodology could be used for operational applications, the only requirement being topographic data for a given field, since it is simple, the algorithms used are unbiased and it could be performed using free software.","palabras_clave":"Site specific managment|Precision farming|Topographic position index (TPI)|Management unit"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3982,"idioma_id":4,"titulo":"Methadone as first-line opioid treatment for cancer pain in a developing country palliative care unit","resumen":"ABSTRACT Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Background: Methadone is increasingly being used to manage cancer pain in developing countries, mainly because of its low cost. However, there exists little information about its use in a palliative care setting. Preliminary data from our Palliative Care Unit (PCU) suggested that Methadone was the most frequent choice as the first- line alternative for patients requiring strong opioids. Objective: To describe the characteristic of prescription of strong opioids as the initial choice to treat moderate to severe cancer pain along the first month of treatment in a Tertiary PCU in Argentina. : To describe the characteristic of prescription of strong opioids as the initial choice to treat moderate to severe cancer pain along the first month of treatment in a Tertiary PCU in Argentina. Methods: Retrospective analysis based on the records of all new opioid- naive patients with moderate to severe cancer pain referred to PC consultation during a twelve-month period, who were indicated strong opioids in the first consult. Collected data included type and dose of opioid, variations in the intensity of pain and need and reasons for opioid rotation (OR). Descriptive statistics were used to summarize the results. : Retrospective analysis based on the records of all new opioid- naive patients with moderate to severe cancer pain referred to PC consultation during a twelve-month period, who were indicated strong opioids in the first consult. Collected data included type and dose of opioid, variations in the intensity of pain and need and reasons for opioid rotation (OR). Descriptive statistics were used to summarize the results. Results: Fifty- six patients met the inclusion criteria. At baseline, most had ECOG score of 3-4, mixed pain and exhibited poor prognostic factors. Methadone was the most frequent opioid accounting for more than two thirds of prescriptions both initially and at the last assessment. The initial and final opioid dose were similar, although significant decrease in the intensity of pain was achieved (p2 Test) and longer interval between the first day and rotation (pt test), than patients on opioids other that Methadone. Reason for rotation for all opioids was mostly opioid- induced neurotoxicity. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Fifty- six patients met the inclusion criteria. At baseline, most had ECOG score of 3-4, mixed pain and exhibited poor prognostic factors. Methadone was the most frequent opioid accounting for more than two thirds of prescriptions both initially and at the last assessment. The initial and final opioid dose were similar, although significant decrease in the intensity of pain was achieved (p2 Test) and longer interval between the first day and rotation (pt test), than patients on opioids other that Methadone. Reason for rotation for all opioids was mostly opioid- induced neurotoxicity. Conclusions: Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. : Our results demonstrate the preference of first line Methadone in the management of cancer related pain at the PCU. In addition, Methadone provided good pain relief with low rate of OR. Several hypothesis for the high rates of use of Methadone are given. Results herein need to be confirmed in a prospective large population- based study. ","palabras_clave":"SUPPORT CARE CANCER|METHADONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3983,"idioma_id":4,"titulo":"Biological Substrate of the Rapid Volumetric Changes Observed in the Human Liver  During the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Approach.","resumen":"BACKGROUND: The associating liver partition and portal vein ligation for stagedhepatectomy (ALPPS) strategy induces rapid future liver remnant (FLR)hypertrophy. Hepatocyte cellular and molecular changes associated with liverhypertrophy during ALPPS remain ill-defined in humans.METHODS: Patients undergoing the ALPPS approach between June 2011 and October2014 were extracted. Biopsies from the FLR were obtained during the first andsecond stages. Hematoxylin-eosin staining and immunohistochemical analysis forexpression of the proliferating cell nuclear antigen (PCNA) and terminaldeoxynucleotidyl transferase dUTP nick end labeling (TUNEL) were performed. Theproliferative index was defined as: PCNA-TUNEL ratio.RESULTS: Eleven of 34 patients treated were studied during both stages. MedianFLR hypertrophy was 104 % in 6 days, with a mean difference between preoperative and postoperative volume of 361 ml (P&#8201;<&#8201;0.001). The mean hepatocyte numberincreased from 52.7 cells\/mm(2) in the first stage to 89.6 cells\/mm(2) in thesecond stage (P&#8201;=&#8201;0.001). PCNA expression increased by 190 % between stages with a linear correlation (r&#8201;=&#8201;0.58) with macroscopic hypertrophy. The proliferativeindex increased from -3.78 cells\/mm(2) in first stage to 2.32 cells\/mm(2) in the second stage (P&#8201;=&#8201;0.034).CONCLUSIONS: The results of the present study indicate that the rapid FLRvolumetric increase observed in ALPPS is accompanied by histological andmolecular features of hepatocyte cell proliferation.","palabras_clave":"ALPPS|LIVER PARENCHYMA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3984,"idioma_id":4,"titulo":"Sex differences in the mechano-energetic effects of genistein on stunned rat and guinea pig hearts","resumen":"Although the phytoestrogen genistein (Gen) is considered protective in cardiovascular diseases, its direct effects on stunned hearts after transient ischemia-reperfusion (I\/R) are unknown. This report studied the effects of 20 &#956;mol\/L Gen on the mechano-calorimetric behaviour during I\/R of rat and guinea pig hearts to evaluate the energetics of Ca2+ homeostasis. Isolated beating hearts were perfused with control Krebs solution inside a calorimeter with or without perfusion of Gen before a transient period of I\/R. Left ventricular pressure development (P) and total heat rate (Ht) were continuously measured. At 37°C, Gen did not change post-ischemic contractile recovery (PICR), but it increased the relaxation rate. However, PICR was reduced in hearts of male rats and guinea pigs at 30°C. Total muscle economy (P\/Ht) showed the same behaviour as P at each temperature. Inhibition of phosphatases with orthovanadate during Gen perfusion prevented a decrease in PICR in male rat hearts, suggesting that this effect is due to tyrosine kinase inhibition. Reperfusing ischemic hearts with 10 mmol\/L caffeine-36 mmol\/L Na+-Krebs induced contracture dependent on the sarcoreticular Ca2+ content. Contracture relaxation depends on mitochondrial Ca2+ uptake and Gen reduced the relaxation rate. Moreover, Gen prevented the increase in Rhod-2 fluorescence (free [Ca2+]m) of rat cardiomyocytes. In guinea pig hearts, Gen maintained ischemic preconditioning, but was reduced by 5-hydroxydecanoate, suggesting the participation of mitochondrial adenosine triphosphate (ATP)-dependent K channels. Results suggest that Gen acts on several mechanisms that regulate myocardial calcium homeostasis and energetics during I\/R, which differ in a temperature- and sex-dependent manner.","palabras_clave":"CALCIUM HOMEOSTASIS|ENERGY EXPENDITURE|GENDER|HEART"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3985,"idioma_id":5,"titulo":"PRIMER REGISTRO DE TERMITEROS FÓSILES PARA EL  PLEISTOCENO TARDÍO DE LA REGIÓN MESOPOTÁMICA  (ARGENTINA): IMPLICANCIAS PALEOAMBIENTALES","resumen":"En esta contribución se da a conocer el primer registro de termiteros fósiles para la región Mesopotámica de Argentina, y su disposición espacial, al tiempo que se discuten sus afinidades taxonómicas e implicancias paleoambientales. Las estructuras fósiles pertenecen a nidificaciones de insectos sociales atribuibles a miembros de la Familia Termitidae, y se ubican en la zona de contacto entre la base de la Fm. Toropí\/Yupoí (Pleistoceno tardío) y el tope de la Fm. Ituzaingó (Mioceno tardío-Plioceno). Se las identifica en los alrededores del Arroyo Toropí (28°36? S y 59°02? W), Departamento Bella Vista, provincia de Corrientes. El estudio de las nidificaciones y su disposición espacial se realizó en cinco campos de termiteros mediante su relevamiento y descripción morfológica.La morfología de las estructuras estudiadas coincide claramente con la de los nidos de termitas, en donde resulta característica una fuerte muralla externa. La caracterización de campo, yacencia, desarrollo, frecuencia, tamaños e interrelaciones de los termiteros fósiles y actuales sugieren una estrecha similitud con montículos del género Cornitermes. La morfología presente muestra también cierta semejanza con Tacuruichnus farinai, descripta para el Piso\/Edad Marplatense (Plioceno) de la región Pampeana. Por otro lado, el análisis fitolítico indicó que las cantidades y las morfologías fitolíticas son semejantes tanto en los termiteros actuales como en los fósiles. Desde un punto de vista paleoambiental, la abundancia de estos termiteros en el contacto entre las mencionadas unidades indicaría la existencia de suelos bien desarrollados, con abundante vegetación graminosa y condiciones húmedas y cálidas conestacionalidad marcada.","palabras_clave":"MESOPOTAMIA|MIOCENO|PLIOCENO|TERMITEROS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3986,"idioma_id":4,"titulo":"Catalogue of Large Magellanic Cloud star clusters observed in the Washington photometric system","resumen":"Aims: The main goal of this study is to compile a catalogue ofthe fundamental parameters of a complete sample of 277 star clusters(SCs) of the Large Magellanic Cloud (LMC) observed in the Washingtonphotometric system. A set of 82 clusters was recently studied by ourteam.  Methods: All the clusters' parameters such as radii,deprojected distances, reddenings, ages, and metallicities were obtainedby applying essentially the same procedures, which are briefly describedhere. We used empirical cumulative distribution functions to examineage, metallicity and deprojected distance distributions for differentcluster subsamples of the catalogue.  Results: Our new sample of82 additional clusters represents about a 40% increase in the totalnumber of LMC SCs observed to date in the Washington photometric system.In particular, we report here the fundamental parameters obtained forthe first time for 42 of these clusters. We found that single LMC SCsare typically older than multiple SCs. Both single and multiple SCsexhibit asymmetrical distributions in log (age). We compared clusterages derived through isochrone fittings obtained using different modelsof the Padova group. Although ages obtained using recent isochrones areconsistent in general terms, we found that there is some disagreement inthe obtained values and their uncertainties.","palabras_clave":"Large Magellanic Cloud|Star cluster|Washington photometric system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3987,"idioma_id":4,"titulo":"The use of Aedes aegypti larvae attractants to enhance the effectiveness of larvicides","resumen":"Aedes aegypti (L.) is an important dengue, chikungunya, and yellow fevervector.Immaturestages of this species inhabit human-made containers placed in residential landscapes, and the application of larvicides inside containers that cannot be eliminated is still considered a priority in control programs. Larvicidal efficacy is influenced by several factors, including the formulation used, the water quality, and the susceptibility of larvae, among others. If an attractant can be incorporated into a slow-release larvicide formulation, it will be feasible to direct the larvae into the source of insecticide and thereby improving its efficacy. We studied the influence of 1-octen-3ol and 3-methylphenol on the rate of Ae. aegypti larvae mortality using the larvicides Bacillus thuringiensis var. israelensis (Bti), temephos, and spinosad. These chemicals were combined with the larvicides mixed with agar during the bioassays. Mortality was registered every 10 min, and a lethal time 50 (LT50) was calculated. The inclusion of the Ae. aegypti larvae attractants with the larvicides into a solid agar matrix improved their efficiency obtaining a strong and marked reduction in the LT50 compared with the use of larvicides alone.","palabras_clave":"AEDES AEGYPTI|MOSQUITO|INSECTICIDE|ATTRACTANT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3988,"idioma_id":4,"titulo":"Biological and Chemical Characterization of a New Larvicide Ovitrap Made of Plastic With Pyriproxyfen Incorporated for Aedes aegypti (Diptera: Culicidae) Control","resumen":"Aedes aegypti (L.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. Ovitraps are containers constructed to imitate Aedes? natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. In addition to their value for vector surveillance, various ovitrap devices have been evaluated as tools for suppressing Ae. aegypti populations. In this study, we performed a biological and chemical characterization of a new ovitrap prototype manufactured by injection molding of low-density polyethylene (LDPE) with the larvicide pyriproxyfen. Our research shows that pyriproxyfen was immediately released from the LDPE into the water of the ovitrap and led to an emergence inhibition of 100% for over 30 weeks. In addition, ovitraps continued to show a high larvicidal activity after over 20 washes. Pyriproxyfen was detectable in the water after 20 s and reached a peak after 24h. Our results show that this ovitrap can be an effective, inexpensive, and low-maintenance tool for Ae. aegypti surveillance and control.","palabras_clave":"OVITRAP|AEDES AEGYPTI|PYRIPROXIFEN|LARVICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3989,"idioma_id":4,"titulo":"Effect of galacto-oligosaccharides: Maltodextrin matrices on the recovery of Lactobacillus plantarum after spray-drying","resumen":"In this work maltodextrins were added to commercial galacto-oligosaccharides (GOS) in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of L. plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20ºC for 12 weeks, or at 30ºC for 6 weeks.The Tgs of GOS:MD matrices were about 20-30o 29 C higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33 and 44% RH at 5, 20 and 30ºC. Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no MD were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5o 34 C. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-dryingstabilization of a potentially probiotic strain in the presence of GOS.","palabras_clave":"galacto-oligosaccharides|maltodextrin|glass transition temperature|spray-drying"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3990,"idioma_id":4,"titulo":"Comparative study on tert-butyl hydroperoxide induced chemiluminescence in bovine, equine and canine erythrocyte lysate","resumen":"The present investigation was carried out to analyze changes in the time course of chemiluminescence intensity in bovine, equine and canine suspension of lysates erythrocytes induced for incubation with t-butyl hydroperoxide (t-BHP). The addition of t-BHP to canine, equine and bovine suspension of lysates erythrocyte resulted in the peroxidation as evidenced by the emission of light. Light emission shows a characteristic kinetic, when the samples are added with t-BHP the emission increased quickly to reach a maximal level after about 10 min for all species, then declined with time. The total chemiluminescence during incubation of suspension of lysates erythrocyte in the three species with t-BHP was higher in canine and equine than in bovine lysed red cells. The main conclusion of this study is that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to experimental pathological situations","palabras_clave":"Erythrocyte Lysate|Tert-butyl hydroperoxide|Chemiluminescence|Comparative"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3991,"idioma_id":5,"titulo":"Prolegómenos en torno a la comunicación de la ciencia","resumen":"El presente artículo aborda la cuestión establecida entre la ciencia y la tecnología, como productos del conocimiento; sin embargo se pretende imponer para los tiempos actuales la palabra compuesta dada en Tecnociencia. Para lo cual se lleva a cabo un recorrido histórico para fundamentar esta alternativa.","palabras_clave":"Ciencia |Tecnología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3992,"idioma_id":5,"titulo":"Stocks y relaciones de estratificación del carbono y nitrógeno en Oxisoles bajo sistemas forestales","resumen":"El objetivo de este trabajo fue evaluar los efectos del desmonte y posterior reforestación con coníferas (Pinus sp.) sobre las reservas de carbono y nitrógeno, y sus relaciones de estratificación en Oxisoles. En el departamento de Oberá (Misiones), con un diseño completamente al azar, se seleccionaron doce reservas de Selva subtropical (S) y doce lotes con forestaciones de Pinus sp. de 20 años de edad (P). En cada uno se realizó un muestreo de suelo, compuesto, aleatorio simple, a 3 profundidades: 0-0,10; 0,10-0,20; 0,20-0,30 m. Las variables evaluadas fueron: textura, pH, densidad aparente (Da), carbono orgánico (COS) y nitrógeno total (N). Se calcularon los stocks de carbono (SC) y de nitrógeno (SN) de 0 a 0,30 m, la tasa de acumulación de carbono (TACOS) y las relaciones de estratificación del COS y del N (COSr y Nr). Los resultados fueron analizados mediante ANOVA, test LSD (p < 0,05), y correlación de Pearson. Los suelos bajo P presentaron menores valores de COS, N, SC y SN, indicando una degradación del suelo. Las COSr fueron mayores en S, tomando valores superiores a 2, denotando una alta calidad de suelo. La TACOS bajo uso forestal fue -0,864 Mg C ha-1 año-1, señalando pérdidas de carbono. La eliminación de la S y su reemplazo por P provocó una pérdida del 28% del SC y del 51% del SN, indicando degradación y pérdida de calidad del suelo por el uso forestal.","palabras_clave":"Uso forestal |Contenidos orgánicos|Tasas de estratificación y de acumulación|Oxisoles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3993,"idioma_id":4,"titulo":"First report of a Maastrichtian palynoflora from the Golfo San Jorge basin, central Patagonia, Argentina","resumen":"This paper reports on the biostratigraphic, paleobiogeographic and paleoecologic aspects of a palynoflora recovered from sedimentary rocks preliminarily assigned to the Lago Colhué Huapi Formation, in the Golfo San Jorge Basin. The present palynological assemblage includes Maastrichtian marker species of the austral Proteacidites\/Nothofagidites Province, such as Quadraplanus brossus and Tubulifloridites lilliei. Typical species of the Maastrichtian paleotropical Palmae Province, Buttinia andreevi and Gabonisporis vigourouxii, are also recognized in the palynoflora. These facts indicate the mixed character of the paleoflora and the prevalence of transitional climatic conditions at the paleolatitude of the Chubut Province in the uppermost Cretaceous. The palynoflora composition suggests the local existence of a warm and low energy freshwater body.","palabras_clave":"Palynomorphs|Upper Cretaceous|Biostratigraphy|San Jorge Gulf Basin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3994,"idioma_id":4,"titulo":"Palaeogeographic changes drove prehistoric fishing practices in the Cambaceres Bay (Tierra del Fuego, Argentina) during the middle and late Holocene","resumen":"Fishing intensification is evidenced in the archaeological record of the Beagle Channel region (Tierra del Fuego, Argentina) during the late Holocene by significant diachronic increases in both the representation of coastal taxa and the diversity of pelagic fish species taken. Faunal evidence from the Imiwaia I site,however, shows a different pattern in temporal variation in the exploitation of coastal fish in contrast to that of the regional trend. By comparing data from palaeogeography and archaeoichthyology, we have been able to evaluate how changes in Holocene coastal geomorphology near the Imiwaia I site influenced hunter?gatherer subsistence. The results show that the abundance and taxonomic diversity recorded in ichthyofaunal assemblages at the Imiwaia I site coincide with the environmental expectations arising from palaeogeographic reconstructions of the Cambaceres Bay during the middle and late Holocene.","palabras_clave":"Ichthyoarchaeology|Palaeogeography|Fishergathererhunters| Middlelate Holocene"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3995,"idioma_id":5,"titulo":"Características Socioeconómicas y Rendimiento Académico. El Caso de una Universidad Argentina","resumen":"El objetivo de la investigación es determinar mediante regresiones estadísticas las relaciones entre las características socioeconómicas de los alumnos de las carreras de grado de la Universidad Nacional del Nordeste (UNNE, Argentina) y sus resultados académicos, y compararlos con los hallazgos de estudios similares efectuados en otras universidades nacionales. Se encuentra evidencia contundente de que las variables de Nivel Socioeconómico (NSE) son significativas para explicar las diferencias de rendimiento académico entre los alumnos de las facultades analizadas. Las variables que se han utilizado como indicadores de NSE elevado han presentado una correlación positiva, y las variables que se emplearon como indicadores de NSE más bajo han presentado una correlación negativa. Las variables sexo femenino, edad, estado civil casado y situación laboral inactiva están asociadas a un mejor rendimiento académico, mientras que las variables cantidad de hijos del estudiante, situación laboral ocupado, situación laboral desocupado, ausencia de cobertura médica y beneficiario de planes sociales están relacionadas a menores desempeños académicos. En materia de género, el hallazgo más relevante se manifiesta en los resultados de las estudiantes mujeres con hijos: su rendimiento es notoriamente inferior al de los estudiantes hombres con hijos.","palabras_clave":"POLITICA EDUCATIVA|RENDIMIENTO ESCOLAR|CARACTERÍSTICAS SOCIECONOMICAS|EDUCACIÓN SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3996,"idioma_id":5,"titulo":"La circulación de saberes psicológicos, psiquiátricos y psicoanalíticos en la Argentina (1900-1993): apuntes historiográficos e históricos.","resumen":"Resumen: Se presenta un modelo historiográfico crítico sobre la circulación de saberes psicológicos, psiquiátricos y psicoanalíticos, que contempla distintos planos de análisis: conexiones individuales e institucionales entre autores y de corrientes locales con otras foráneas; los medios específicos y las asimetrías en el intercambio de saberes; procesos de lectura, apropiación y uso de los mismos, así como su transformación según las características locales. Se ofrecen los resultados de este modelo historiográfico en investigaciones en la Argentina durante el siglo XX.","palabras_clave":"historia trasnacional|circulación|recepción|psicología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3997,"idioma_id":4,"titulo":"Effect of Nanoclustering and Dipolar Interactions in Heat Generation for Magnetic Hyperthermia","resumen":"Biomedical magnetic colloids commonly used in magnetic hyperthermia experiments often display a bidisperse structure, i.e., are composed of stable nanoclusters coexisting with well-dispersed nanoparticles. However, the influence of nanoclusters in the optimization of colloids for heat dissipation is usually excluded. In this work, bidisperse colloids are used to analyze the effect of nanoclustering and long-range magnetic dipolar interaction on the magnetic hyperthermia efficiency. Two kinds of colloids, composed of magnetite cores with mean sizes of around 10 and 18 nm, coated with oleic acid and dispersed in hexane, and coated with meso-2,3-dimercaptosuccinic acid and dispersed in water, were analyzed. Small-angle X-ray scattering was applied to thoroughly characterize nanoparticle structuring. We proved that the magnetic hyperthermia performances of nanoclusters and single nanoparticles are distinctive. Nanoclustering acts to reduce the specific heating efficiency whereas a peak against concentration appears for the well-dispersed component. Our experiments show that the heating efficiency of a magnetic colloid can increase or decrease when dipolar interactions increase and that the colloid concentration, i.e., dipolar interaction, can be used to improve magnetic hyperthermia. We have proven that the power dissipated by an ensemble of dispersed magnetic nanoparticles becomes a nonextensive property as a direct consequence of the long-range nature of dipolar interactions. This knowledge is a key point in selecting the correct dose that has to be injected to achieve the desired outcome in intracellular magnetic hyperthermia therapy.","palabras_clave":"dipolar interactions|magnetic colloids|radiofrequency magnetic field|magnetic hyperthermia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3998,"idioma_id":4,"titulo":"Controlled deposition of Pd and In on carbon fibers by sequential electroless plating for the catalytic reduction of nitrate in water","resumen":"Catalysts containing 1.8 wt.% of Pd and different In loadings were prepared by sequential electroless plating on activated carbon felts (ACF). Homogeneous structures were obtained, with In particles deposited at the top of a tiny Pd film. The catalyst with the higher In loading (Pd:In ratio of 2.0) presented high activity and good selectivity towards nitrogen, with negligible deactivation after 6 h of time-on-stream and three consecutive nitrate pulses. Besides the promising catalytic behavior, PdIn\/ACF have the advantages of an open structure, which is highly accessible to the reactants, with no need of separation after the reaction.","palabras_clave":"PdIn\/activated carbon felts|Electroless plating|Nitrate reduction|Water purification"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":3999,"idioma_id":4,"titulo":"New records of bats (Chiroptera, Mammalia) from Argentina","resumen":"We provide new distributional records for five species of bats from Argentina, of the families Vespertilionidae and Molossidae, representing new records for Catamarca, Chaco, La Rioja, Misiones, and San Juan provinces, as well as unpublished data for several phytogeographic regions of the country. This information adds important extensions to the distribution of Eumops glaucinus (Wagner, 1843) in Argentina.","palabras_clave":"Chiroptera|Distribution|Molossidae|Vespertilionidae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4000,"idioma_id":4,"titulo":"The tumor antigen N-Glycolyl-GM3 is a human CD1d ligand capable of mediating B cell and natural killer T cell interaction","resumen":"The expression of ganglioside N-glicolil GM3 (NGcGM3) in human is restricted to cancer cells, by definition a tumour antigen (Ag). There are measurable quantities of circulating aNGcGM3 antibodies (Ab) in human serum. Interestingly, some people have circulating Ag specific IgGs, capable of complement-mediated cytotoxicity against NGcGM3 positive cells, relevant for tumour surveillance. In light of the Ag?s chemical nature, we postulated it as a candidate ligand for CD1d. Furthermore, we hypothesize that the immune mechanism involved in the generation of such antibodies entailed a cross talk between B lymphocytes (Bc) and iNKT cells.   Combining cellular techniques as flow cytometry and a non-cellular biochemical assay, we demonstrated that CD1d binds NGcGM3 and that human Bc present NGcGM3 in a CD1d context, by two alternative strategies. We also showed that a fraction of that binding needs living cells. Finally, by co culturing primary human Bc with iNKT cells and measuring Ki 67 expression, we detected a reproducible increment in proliferation of both cell populations when Ag was on the medium.  Our findings identify a novel, endogenous, human CD1d ligand. Moreover, they suggest that expression of NGcGM3 on tumor cells may prime an effective iNKT dependent antitumor antibody response, through cooperation with B cells, an immunological mechanism that has not been described before for humans.","palabras_clave":"gangliosides|cooperation|B Cells|NKT cells"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4001,"idioma_id":4,"titulo":"The early onset of type 1 autoimmune hepatitis has a strong genetic influence: role of HLA and KIR genes","resumen":"We have previously reported a strong association between HLA-DRB1*1301 and type 1 pediatric autoimmune hepatitis (PAH) and between HLA-DR*0405 and adult autoimmune hepatitis (AAH). Because human killer cell immunoglobulin-like receptors are known to be associated with susceptibility to autoimmune diseases, we investigated the frequencies of HLA-A, B, C, DRB1 and KIR genes in 144 type 1 PAH and 86 AAH patients, which were compared with 273 healthy controls. We demonstrated in PAH the increased frequency of the functional form of KIR2DS4-Full Length (KIR2DS4-FL), which in combination with HLA-DRB1*1301 revealed a strong synergistic effect (odds ratio=36.5). PAH-KIR2DS4-FL+ subjects have shown an increased frequency of their putative HLA-C*02, 04 and 06 ligands. KIR analysis of PAH also revealed a decreased frequency of KIR2DL2 gene and its ligand. In contrast, AAH cases have shown a weaker increased frequency of KIR2DS4-FL, a lack of synergistic effect with HLA class II antigens and a moderate association with HLA-DRB1*0405. Of note, we demonstrated that liver T cells have a unique pattern of KIR expression. These results show a KIR gene involved in autoimmune hepatitis and suggest a stronger genetic influence for the early onset type I autoimmune hepatitis.","palabras_clave":"AUTOIMMUNE HEPATITIS|HLA|KIR GENES|NK CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4002,"idioma_id":4,"titulo":"ATP-Induced Inflammation Drives  Tissue-Resident Th17 Cells in Metabolically  Unhealthy Obesity","resumen":"Obesity induced inflammation is conducted by a metabolic pathway, which eventually causes activation of specialized immune cells and leads to an unresolved inflammatory response within the tissue. For this reason, it is critically important to determine how hypertrophic fat tissue alters T cell balance to drive inflammation. In the present study, we identify the purinergic signaling as a novel mechanism driving the adaptive Th17 response in human visceral adipose tissue (VAT) of metabolically unhealthy obese patients (MUO). We demonstrate that ATP acting via the P2X7 receptor pathway promotes a Th17 polarizing microenvironment with high levels of IL-1&#946;, IL-6 and IL-17 in VAT explants from lean donors. Moreover, in vitro blockade of the P2X7 receptor abrogates the levels of these cytokines. These findings are consistent with a greater frequency of Th17 cells in tissue from MUO donors, revealed not only by the presence of a baseline Th17 promoting milieu, but also by the higher expression of steadily recognized Th17 markers as RORC, IL-17 cytokine and IL-23 receptor, in comparison with metabolically healthy obese and lean donors. Additionally, we demonstrate that CD39 expression on CD4+ effector T cells represents a novel Th17 marker in the inflamed VAT which also confers protection against ATP-induced cell death. The manipulation of the purinergic signaling might represent a new therapeutic target to shift the CD4+ T cell balance under inflammatory conditions.","palabras_clave":"Obesidad|Tregs|Th17|ATP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4003,"idioma_id":5,"titulo":"Modelo Conceptual Para la Gestión de Variabilidad Temporal  de Familias de Productos","resumen":"Dada la naturaleza de diversos eventos que modifican la información de una familia de productos, la gestión de ciclo de vida de productos o PLM (Product Lifecycle Management) requiere de soluciones robustas que registren los cambios ocurridos y que gestionen la información de familia de producto. El objetivo del trabajo es presentar un modelo conceptual para gestionar la variabilidad en el tiempo de una familia de productos, manteniendo la integridad y la consistencia de los modelos de datos involucrados. Esta conceptualización sirve como herramienta para referenciar las versiones de una familia de productos, recuperar información y representar los cambios. La propuesta se aplicará a dos modelos de representación de productos de diferentes dominios, tales como: PRONTO (PRoduct ONTOlogy) y el Modelo de Características (Feature Model - FM).","palabras_clave":"Gestión de Versiones|Familias de Producto|Ontologías|Variabilidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4004,"idioma_id":4,"titulo":"Brain network organization predicts social executive performance in frontotemporal dementia","resumen":"Objectives: Behavioral variant frontotemporal dementia (bvFTD) is characterized by early atrophy in the frontotemporoinsular regions. These regions overlap with networks that are engaged in social cognition-executive functions, two hallmarks deficits of bvFTD. We examine (i) whether Network Centrality (a graph theory metric that measures how important a node is in a brain network) in the frontotemporoinsular network is disrupted in bvFTD, and (ii) the level of involvement of this network in social-executive performance. Methods: Patients with probable bvFTD, healthy controls, and frontoinsular stroke patients underwent functional MRI resting-state recordings and completed social-executive behavioral measures. Results: Relative to the controls and the stroke group, the bvFTD patients presented decreased Network Centrality. In addition, this measure was associated with social cognition and executive functions. To test the specificity of these results for the Network Centrality of the frontotemporoinsular network, we assessed the main areas from six resting-state networks. No group differences or behavioral associations were found in these networks. Finally, Network Centrality and behavior distinguished bvFTD patients from the other groups with a high classification rate. Conclusions: bvFTD selectively affects Network Centrality in the frontotemporoinsular network, which is associated with high-level social and executive profile. (JINS, 2016, 22, 250?262)","palabras_clave":"Functional connectivity|Graph theory analysis|Frontoinsularstroke|Neurodegenerative disease|fMRI resting-state"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4005,"idioma_id":4,"titulo":"Structural Insights into the HWE Histidine Kinase Family: The Brucella Blue Light-Activated Histidine Kinase Domain","resumen":"In response to light, as part of a two-component system, the Brucella blue light-activated histidine kinase (LOV-HK) increases its autophosphorylation, modulating the virulence of this microorganism. The Brucella histidine kinase (HK) domain belongs to the HWE family, for which there is no structural information. The HWE family is exclusively present in proteobacteria and usually coupled to a wide diversity of light sensor domains. This work reports the crystal structure of the Brucella HK domain, which presents two different dimeric assemblies in the asymmetric unit: one similar to the already described canonical parallel homodimers (C) and the other, an antiparallel non-canonical (NC) dimer, each with distinct relative subdomain orientations and dimerization interfaces. Contrary to these crystallographic structures and unlike other HKs, in solution, the Brucella HK domain is monomeric and still active, showing an astonishing instability of the dimeric interface. Despite this instability, using cross-linking experiments, we show that the C dimer is the functionally relevant species. Mutational analysis demonstrates that the autophosphorylation activity occurs in cis. The different relative subdomain orientations observed for the NC and C states highlight the large conformational flexibility of the HK domain. Through the analysis of these alternative conformations by means of molecular dynamics simulations, we also propose a catalytic mechanism for Brucella LOV-HK.","palabras_clave":"HISTIDINE KINASE|LOV|PHOTORECEPTOR|BRUCELLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4006,"idioma_id":4,"titulo":"Structure of the full-length bacteriophytochrome from the plant pathogen Xanthomonas campestris provides clues to its long-range signaling mechanism","resumen":"Phytochromes constitute a major superfamily of light-sensing proteinsthat are reversibly photoconverted between a red-absorbing (Pr) and afar-red-absorbing (Pfr) state. Bacteriophytochromes (BphPs) are found among photosynthetic and non-photosynthetic bacteria, including pathogens. To date,several BphPs have been biophysically characterized. However, it is still not fully understood how structural changes are propagated from the photosensory module to the output module during the signal transduction event. Most phytochromes share a common architecture consisting of an N-terminal photosensor that includes the PAS2-GAF-PHY domain triad and a C-terminal variable output module. Here we present the crystal structure of the full-length BphP from the plant pathogen Xanthomonas campestris pv. campestris (XccBphP) bearing its photosensor and its complete output module, a PAS9 domain. In the crystals, the protein was found to be in the Pr state, whereas diffraction data together with resonance Raman spectroscopic and theoretical results indicate a ZZZssa and a ZZEssa chromophore configuration corresponding to a mixture of Pr and Meta-R state, the precursor of Pfr. The XccBphP quaternary assembly reveals a head-to-head dimer in which the output module contributes to the helical dimer interface. The photosensor, which is shown to be a bathy-like BphP, is influenced in its dark reactions by the output module. Our structural analyses suggest that the photoconversion between the Pr and Pfr states in the full-length XccBphP may involve changes in the relative positioning of the output module. This work contributes to understand the light-induced structural changes propagated from the photosensor to the output modules in phytochrome signaling.","palabras_clave":"XANTHOMONAS CAMPESTRIS PV CAMPESTRIS|PHYTOCHROME|BILIVERDIN|RED-LIGHT PHOTORECEPTOR|SIGNAL TRANSDUCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4007,"idioma_id":4,"titulo":"Mycobacterium paratuberculosis detection in cow's milk in Argentina by immunomagnetic separation-PCR","resumen":"The aim of this study was to standardize a diagnosis procedure to detect Mycobacterium avium subsp. paratuberculosis (Map) DNA in raw cow milk samples under field conditions. A procedure that combines both immunomagnetic separation and IS900-PCR detection (IMS-IS1 PCR) was employed on milk samples from 265 lactating Holstein cows from Map infected and uninfected herds in Argentina. IMS-IS1 PCR results were analyzed and compared with those obtained from milk and fecal culture and serum ELISA. The extent of agreement between both tests was determined by the Kappa test. IMS-IS1 PCR showed a detection limit of 10(1) CFU of Map\/mL of milk, when 50:50 mix of monoclonal and polyclonal antibodies were used to coat magnetic beads. All of the 118 samples from the Map uninfected herds were negative for the set of the tests. In Map infected herds, 80 out of 147 cows tested positive by milk IMS-IS1 PCR (55%), of which 2 (1.4%) were also positive by milk culture, 15 (10%) by fecal culture, and 20 (14%) by serum ELISA. Kappa statistics (95% CI) showed a slight agreement between the different tests (<0.20), and the proportions of agreement were &#8804;0.55. The IMS-IS1 PCR method detected Map in milk of the cows that were not positive in other techniques. This is the first report dealing with the application of IMS-IS1 PCR in the detection of Map in raw milk samples under field conditions in Argentina.","palabras_clave":"Cow's milk|Immunomagnetic separation|Mycobacterium avium subsp. paratuberculosis|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4008,"idioma_id":5,"titulo":"Estudio microestructural y de texturas en aleación Fe43.5Mn34Al15Ni7.5 con memoria de forma","resumen":"La aleación Fe43.5Mn34Al15Ni7.5, descubierta recientemente, presenta comportamiento pseudoelástico en un amplio rango de temperaturas. Esta característica resulta atractiva para aplicaciones automovilísticas, espaciales,criogénicas y sísmicas. En la actualidad no existe un análisis exhaustivo de las distintas fases que pueden generarse en la aleación con los tratamientos térmicos y de la influencia de las texturas sobre las propiedades de memoria de forma. El material estudiado fue laminado en caliente a una temperatura de 1000ºC y luego tratado térmicamente a diferentes temperaturas a fin de analizar las fases presentes, su microestructura y sus orientaciones cristalinas. Se determinó que la fase martensítica &#947;&#61620; se logra con temples desde temperaturassuperiores a los 1200°C. Además, bajo tratamientos térmicos por encima de 1000°C se obtienen tamaños de grano en la fase &#945; cercanos al mm, resultando a priori en una mejora de las propiedades pseudoelásticas de la aleación.","palabras_clave":"pseudoelasticidad,|transformación de fase|composición|tratamientos térmicos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4009,"idioma_id":4,"titulo":"Loss of myocardial protection against myocardial infarction in middle aged transgenic mice overexpressing cardiac thioredoxin-1","resumen":"ABSTRACTThioredoxin-1 (Trx1) protects the heart from ischemia\/reperfusion (I\/R) injury. Given that the age at which the first episode of coronary disease takes place has considerably decreased, life at middle-aged (MA) emerges as a new field of study. The aim was determine whether infarct size, Trx1 expression and activity, Akt and GSK-3&#946; were altered in young (Y) and MA mice overexpressing cardiac Trx1, and in a dominant negative (DN-Trx1) mutant of Trx1. Langendorff-perfused hearts were subjected to 30 minutes of ischemia and 120 minutes of reperfusion (R). We used 3 and 12 month-old male of wild type (WT), Trx1, and DN-Trx1. Trx1 overexpression reduced infarct size in young mice (WT-Y: 46.8±4.1% vs. Trx1-Y: 27.6±3.5%, p < 0.05). Trx1 activity was reduced by 52.3±3.2% (p < 0.05) in Trx1-MA, accompanied by an increase in nitration by 17.5±0.9%, although Trx1 expression in transgenic mice was similar between young and middle-aged. The expression of p-Akt and p-GSK-3&#946; increased during reperfusion in Trx1-Y. DN-Trx1 mice showed neither reduction in infarct size nor Akt and GSK-3&#946; phosphorylation. Our data suggest that the lack of protection in Trx1 middle-aged mice even with normal Trx1 expression may be associated to decreased Trx1 activity, increased nitration and inhibition of p-Akt and p-GSK-3&#946;.","palabras_clave":"myocardial|protection|infarction|thioredoxin-1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4010,"idioma_id":5,"titulo":"DESCRIPCION ANATOMICA DE LA MUSCULATURA DEL MIEMBRO PELVIANO DEL CARPINCHO (Hydrochoerus hydrochaeris)","resumen":"El 'carpincho' es un roedor de gran talla ampliamente distribuido en la zona de llanos y humedales de Sudamérica. La información anatómica sobre la musculatura de esta especie es escasa. El objetivo del ensayo fue describir las estructuras musculares de su extremidad pelviana. Se utilizaron los miembros pelvianos de cuatro cadáveres (hembras). Los especímenes fueron conservados mediante métodos anatómicos tradicionales. Tras separar los miembros, se retiró la piel, la grasa subcutánea y las fascias. Luego se realizó la disección de las diferentes regiones musculares, para determinar el origen e inserción de cada músculo, datos que se avalaron con registros fotográficos. Las observaciones revelaron que los músculos presentes son los mismos que los descriptos en otros mamíferos pentadáctiles, destacándose el gran desarrollo de los músculos de la región del pie. Se espera que los estudios anatómicos de H. hydrochaeris contribuyan a mejorar su manejo sanitario y productivo.","palabras_clave":"CARPINCHO|ANATOMIA|HYDROCHOERUS HYDROCHAERIS|MÚSCULOS MIEMBRO PELVIANO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4011,"idioma_id":4,"titulo":"Sialic Acid Glycobiology Unveils Trypanosoma cruzi Trypomastigote Membrane Physiology","resumen":"Trypanosoma cruzi, the flagellate protozoan agent of Chagas disease or American trypanosomiasis, is unable to synthesize sialic acids de novo. Mucins and trans-sialidase (TS) are substrate and enzyme, respectively, of the glycobiological system that scavenges sialic acid from the host in a crucial interplay for T. cruzi life cycle. The acquisition of the sialyl residue allows the parasite to avoid lysis by serum factors and to interact with the host cell. A major drawback to studying the sialylation kinetics and turnover of the trypomastigote glycoconjugates is the difficulty to identify and follow the recently acquired sialyl residues. To tackle this issue, we followed an unnatural sugar approach as bioorthogonal chemical reporters, where the use of azidosialyl residues allowed identifying the acquired sugar. Advanced microscopy techniques, together with biochemical methods, were used to study the trypomastigote membrane from its glycobiological perspective. Main sialyl acceptors were identified as mucins by biochemical procedures and protein markers. Together with determining their shedding and turnover rates, we also report that several membrane proteins, including TS and its substrates, both glycosylphosphatidylinositol-anchored proteins, are separately distributed on parasite surface and contained in different and highly stable membrane microdomains. Notably, labeling for &#945;(1,3)Galactosyl residues only partially colocalize with sialylated mucins, indicating that two species of glycosylated mucins do exist, which are segregated at the parasite surface. Moreover, sialylated mucins were included in lipid-raft-domains, whereas TS molecules are not. The location of the surface-anchored TS resulted too far off as to be capable to sialylate mucins, a role played by the shed TS instead. Phosphatidylinositol-phospholipase-C activity is actually not present in trypomastigotes. Therefore, shedding of TS occurs via microvesicles instead of as a fully soluble form.","palabras_clave":"TRYPANOSOMA|MUCIN|TRYPOMASTIGOTE|FLAGELLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4012,"idioma_id":4,"titulo":"Mediators and molecular pathways involved in the regulation of neutrophil extracellular trap formation mediated by activated platelets","resumen":"In addition to being key elements in hemostasis and thrombosis, platelets amplify neutrophil function. We aimed to gain further insight into the stimuli,mediators, molecular pathways, and regulation of neutrophil extracellular trap formation mediated by human platelets. Platelets stimulated by lipopolysaccharide, a wall component of gram-negative bacteria, Pam3-cysteineserine- lysine 4, a mimetic of lipopeptide from gram-positive bacteria, Escherichia coli, Staphylococcus aureus, or physiologic platelet agonists promoting neutrophil extracellular trap formation and myeloperoxidase-associated DNA activity under static and flow conditions. Although P-selectin or glycoprotein IIb\/IIIa were not involved, platelet glycoprotein Ib, neutrophil cluster of differentiation 18, and the release of von Willebrand factor and platelet factor 4 seemed to be critical for the formation of neutrophil extracellular traps. The secretion of these molecules depended on thromboxane A2 production triggered by lipopolysaccharide or Pam3-cysteine-serine-lysine 4 but not on high concentrations of thrombin. Accordingly, aspirin selectively inhibited platelet-mediated neutrophil extracellular trap generation. Signaling through extracellular signal-regulated kinase, phosphatidylinositol 3-kinase, and Src kinases, but not p38 or reduced nicotinamide adenine dinucleotide phosphate oxidase, was involved in platelet-triggered neutrophil extracellular trap release. Platelet-mediated neutrophil extracellular trap formation was inhibited by prostacyclin. Our results support a role for stimulated platelets in promoting neutrophil extracellular trap formation, reveal that an endothelium-derived molecule contributes to limiting neutrophil extracellular trap formation, and highlight platelet inhibition as a potential target for controlling neutrophil extracellular trap cell death.","palabras_clave":"NETS|PLAQUETAS|TLR|EXTRACELULAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4013,"idioma_id":4,"titulo":"Catalytic  pyrolysis  of  cellulose  using  MCM-41  type  catalysts","resumen":"The pyrolysis of microcrystalline cellulose in contact with MCM-41 and Fe&#8722;, Al&#8722; and Cu&#8722; substituted samples was carried out at 400 °C in a fixed-bed glass reactor in order to obtain high bio-oils yields. The acid properties of the catalysts were determined by potentiometric titration with n-butylamine, while the redox properties were studied by TPR. BET surface area, pore volume and pore size were measured by means of N2 sorptometry. The effect of the different catalysts on the composition of the bio-oils was analyzed in the light of the characterization results. Mainly the concentration of levoglucosan (LG), levoglucosenone (LGO), 1,4:3,6-dianhydro-&#946;-d-glucopyranose (DGP) and (1S,5R)-5-hydroxy-3,7-dioxabicyclo[3.2.1]octan-4-one (LAC) were measured. The presence of the solid catalysts increased the liquid formation and strongly modified the chemical composition of the bio-oils compared to the non-catalytic pyrolysis. All the catalysts decreased the production of LG and increased that of LGO, LAC and DPG. The mild acidity of the catalysts promoted dehydration reactions, while redox properties led to a higher production of isomerization products. A much higher LGO yield was obtained over Al-MCM-41 (53 wt% of cellulose is converted to LGO over this catalyst) than by non-catalytic pyrolysis of cellulose previously washed with phosphoric acid (7 wt% of washed cellulose is converted to LGO). In order to evaluate the reuse of the catalyst, the spent sample was submitted to calcination at 400 °C. The physicochemical properties were not modified after the calcination treatment and the pyrolysis employing the spent sample led to a similar bio-oil composition (though with a smaller yield) as in the case of the fresh catalyst. A second regeneration of the catalyst diminished the specific surface area and the surface acidity. Concomitantly a notable decrease of the yield of LGO was observed.","palabras_clave":"Catalytic pyrolysis|CELLULOSE|MCM-41|BIO-OIL|LEVOGLUCOSENONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4014,"idioma_id":4,"titulo":"Spatial patterns of cultural ecosystem services provision in Southern Patagonia","resumen":"Although there is a need to develop a spatially explicit methodological approach that addresses the social importance of cultural ecosystem services for regional planning, few studies have analysed the spatial distribution on the cultural ecosystem services based on social perceptions. The main objective of this study was to identify cultural ecosystem service hot-spots, and factors that characterize such hot-spots and define the spatial associations between cultural ecosystem services in Southern Patagonia (Argentina). The study was carried out in Southern Patagonia (243.9 thousand km²) located between 46° and 55° SL with the Andes mountains on the western fringe and the Atlantic Ocean on the eastern fringe of the study area.  The study region has a range of different vegetation types (grasslands, shrub-lands, peat-lands and forests) though the cold arid steppe is the main vegetation type. We used geo-tagged digital images that local people and visitors posted in the Panoramio web platform to identify hot-spots of four cultural ecosystem services (aesthetic value, existence value, recreation and local identity) and relate these hot-spots with social and biophysical landscape features. Aesthetic value was the main cultural service tagged by people, followed by the existence value for biodiversity conservation, followed by local identity and then recreational activity. The spatial distribution of these cultural ecosystem services are associated with different social and biophysical characteristics, such as the presence of water bodies, vegetation types, marine and terrestrial fauna, protected areas, urbanization, accessibility and tourism offer. The most important factors are the presence of water in Santa Cruz and tourism offer in Tierra del Fuego. Our results demonstrate that this methodology is useful for assessing cultural ecosystem services at the regional scale, especially in areas with low data availability and field accessibility, such as Southern Patagonia. We also identify new research challenges that can be addressed in cultural ecosystem services research through the use of this method.","palabras_clave":"SOCIAL PERCEPTIONS &#61599;  &#61599; &#61599; | EXISTENCE VALUE|AESTHETIC VALUE|LOCAL IDENTITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4015,"idioma_id":4,"titulo":"Why biodiversity increases after variable retention harvesting: A meta-analysis for southern Patagonian forests","resumen":"Effects of forest harvesting on biodiversity can be varied and complex to understand. We provide a metaanalysis of 553 studies plants, insects and birds to identify the general responses to Variable Retention harvesting (VR) 1?8 years post-harvest in Nothofagus pumilio forests of southern Patagonia. The analysis is focused on: (i) richness and abundance, (ii) origin and habitat (native forest specialist species, native species of other habitats, alien species), and (iii) temporal trends after harvesting. Our objective was to evaluate why biodiversity increases after variable retention harvesting, by assessing the effects on (i) species richness and abundance in general, (ii) native forest specialists, native generalist species and alien species, and (iii) the recovery of biodiversity toward original conditions. Forests managed with VR supported higher overall richness and abundance of plants, insects and birds in aggregate and dispersed retention than unmanaged stands, but with similar values each other. However, origin and habitat of species affected responses to VR. Aggregates support higher native forest specialist plant and lower plants of habitats other than dispersed retention. However, both retention treatments increased alien plants, although its richness and abundance was higher in dispersed retention. Native forest specialist insects were reduced in comparison to unmanaged forest, while insects of other habitats showed a positive response to both aggregate and dispersed retention as well as did for bird species richness and abundance compared to unmanaged forests. We found evidence for recovery of original conditions for native forest specialist plants and insects, and plants of other habitats. In contrast, alien plants and native insects of other habitats increased continuously in the studies included through those representing 8 years postharvest. Major differences among both retention patterns included significantly higher richness and abundance of alien plants and native insects of other habitats in dispersed retention. Our synthesis shows recovery toward original conditions for some taxa, but demonstrates long-term establishment of alien plants as well as insect species not associated with native N. pumilio forests. These have emerged as a main potential threat to conservation of forests under VR prescription, at least at the stand level. Retention forestry could play a fundamental role for conservation in productive temperate forests, but the influence of retention pattern and aggregate size are still unclear.","palabras_clave":"BIOLOGICAL CONSERVATION  |DISPERSED RETENTION|FOREST SPECIALIST SPECIES |POST-HARVEST RECOVERY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4016,"idioma_id":4,"titulo":"Aptian-Albian Cupressaceae (sensu stricto) woods from Cañad\u0002ón Asfalto Basin, Patagonia Argentina","resumen":"This paper describes the first macrofloristic record from Los Adobes Formation (Chubut Group) in Cañaadon Asfalto Basin (Patagonia, Chubut Province, Argentina). The studied fossils were recovered fromthe AptianeAlbian Bardas Coloradas Member, and consist of silicified woods preserved in fluvial channel deposits. One wood shows homocellular rays with smooth radial and tangential walls, diffuse axial parenchyma, tracheid pitting of the abietinoid type and cross-field pitting cupressoid usually ordered in rows and columns indicating placement within Cupressinoxylon hallei Kr?ausel. This fossil tree resemblessome members of the extant Cupressaceae s.s., as Austrocedrus chilensis (D. Don) Pic. Serm. &amp; Bizzarri, Pilgerodendron uviferum (D. Don) Florin, Fitzroya cupressoide (Molina) I. M. Johnst. and Libocedrus plumosa (D. Don) Druce. Due to poor preservation the other fossil wood specimen was assigned to cf. Cupressinoxylon Gooppert 1850 nom. cons. prop. This is the first unequivocal record of C. hallei from the Cretaceous of South America and the oldest reliable record of the Callitroideae, indicating that this subfamily was well established since the mid-Cretaceous in the Southern Hemisphere.","palabras_clave":"CUPRESSACEA|LOS ADOBES FORMATION|APTIAN-ALBIAN|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4017,"idioma_id":4,"titulo":"Fuzzy logic-based assessment for mapping potential infiltration areas in low-gradient watersheds","resumen":"This paper gives an account of the design a logic-based approach for identifying potential infiltrationareas in low-gradient watersheds based on remote sensing data. This methodological framework isapplied in a sector of the Pampa Plain, Argentina, which has high level of agricultural activities and largedemands for groundwater supplies. Potential infiltration sites are assessed as a function of two primarytopics: hydrologic and soil conditions. This model shows the state of each evaluated subwatershedrespecting to its potential contribution to infiltration mainly based on easily measurable and commonlyused parameters: drainage density, geomorphologic units, soil media, land-cover, slope and aspect (slopeorientation). Mapped outputs from the logic model displayed 42% very low-low, 16% moderate, 41% highveryhigh contribution to potential infiltration in the whole watershed. Subwatersheds in the upper andlower section were identified as areas with high to very high potential infiltration according to thefollowing media features: low drainage density (<1.5 km\/km2), arable land and pastures as the mainland-cover categories, sandy clay loam to loam - clay loam soils and with the geomorphological unitsnamed poorly drained plain, channelized drainage plain and, dunes and beaches.","palabras_clave":"Infiltration|Fuzzy logic|Remote sensing|Watershed management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4018,"idioma_id":4,"titulo":"Snowmelt Contribution to the Sustainability of the Irrigated Mendoza's Oasis, Argentina: an isotope study","resumen":"Mendoza is the province that has the largest irrigated area in Argentina where water resources depend mainly on snowmelt and glacier melting in the closed Andes Mountain. In this region the Blanco River Basin is one of the most important, covering about 300 km2 flowing from the highest peaks to Potrerillos Dam (1300 masl). The objective of this work is to make a preliminary characterization of stable isotopes in surface and groundwater, ice and snowmelt in order to contribute to a better understanding of the hydrologic cycle in the region, by using a Los Gatos DLT-100 analyzer. Hydrochemical analyses were performed on 157 samples. The isotopic composition of rain water is more enriched than streamwater clearly indicating that the stream recharge sources are at higher altitude. The discharge regime of the streams indicates that snowmelt is the main water origin, and considering the theoretical evolution of isotopes in meltwater, the composition of streams corresponds to the first meltwater, without fractionation. Then, it is more depleted than the original snow. The ice isotopic composition is more enriched than streamwater, indicating that snowmelt is the main recharge source. The hydrochemical results indicate the importance of geology in determining streamwater composition, which is integrated with isotopic data in order to achieve a better comprehension of the hydrological system","palabras_clave":"isotopes|groundwater| surface water| snowmelt"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4019,"idioma_id":4,"titulo":"Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Reversion or promotion of malignancy by inducing melanogenesis or metastasis","resumen":"Advanced melanoma is the most aggressive form of skin cancer. It is highlymetastatic and dysfunctional in melanogenesis; two processes that are induced byH2O2. This work presents a melanoma cell model with low levels of H2O2 inducedby catalase overexpression to study differentiation\/dedifferentiation processes.Three clones (A7, C10 and G10) of human A375 amelanotic melanoma cells withquite distinct phenotypes were obtained. These clones faced H2O2 scavenging by two main strategies. One developed by clone G10 where ROS increased. This resulted in G10 migration and metastasis associated with the increased of cofilin-1 and CAP1. The other strategy was observed in clone A7 and C10, where ROS levels were maintained reversing malignant features. Particularly, C10 was not tumorigenic, while A7 reversed the amelanotic phenotype by increasing melanin content and melanocytic differentiation markers. These clones allowed the study of potential differentiation and migration markers and its association with ROS levels in vitro and in vivo, providing a new melanoma model with different degree of malignancy.","palabras_clave":"MELANOMA|CATALASE|ROS|MELANOGENESIS|METASTASIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4020,"idioma_id":4,"titulo":"Pharmacokinetics of Immediate and Sustained Release  Cephalexin Administered by Different Routes to Llamas (Lama  glama)","resumen":"We investigate the pharmacokinetics of two different cephalexin formulations administered to llamas by the intravenous (IV), intramuscular (IM), and subcutaneous (SC) routes, the minimum inhibitory concentration (MIC) of cephalexin against some Escherichia coli and staphylococci isolated from llamas, and we apply the PK\/PD modelling approach, so that effective dosage recommendations for this species could be made. Six llamas received immediate (10&#8201;mg\/kg, IV, IM, and SC) and sustained (8&#8201;mg\/kg IM, SC) release cephalexin. Pharmacokinetic parameters were calculated by noncompartmental approach. Immediate release SC administration produced a significantly longer elimination half-life as compared with the IV and IM administration ( versus  and &#8201;h, resp.) and higher mean absorption time as compared with the IM administration ( versus &#8201;h). Absolute bioavailability was in the range of 72?89% for both formulations and routes of administration. Cephalexin MIC90 values against staphylococci and E. coli were 1.0 and 8.0&#8201;&#956;g\/mL, respectively. Our results show that the immediate release formulation (10&#8201;mg\/kg) would be effective for treating staphylococcal infections administered every 8&#8201;h (IM) or 12&#8201;h (SC), whereas the sustained release formulation (8&#8201;mg\/kg) would require the IM or SC administration every 12 or 24&#8201;h, respectively.","palabras_clave":"pharmacokinetics|betalactams|Lama glama|cephalexin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4021,"idioma_id":5,"titulo":"PERCEPCIONES Y VALORACIONES PERIODISTICAS EN TORNO DEL PATRIMONIO ARQUEOLOGICO. EL CASO DE OLAVARRIA, ARGENTINA","resumen":"La circulación del conocimiento arqueológico en los medios de comunicación local es importante para contribuir a su visibilidad social. En este sentido, los medios de comunicación son considerados espacios de educación no formal por su influencia en la opinión pública. Esta comunicación está mediada por los periodistas, que son vistos como actores clave. Aquí sus opiniones y percepciones se analizan en relación con la arqueología y el patrimonio arqueológico en la ciudad de Olavarría, provincia de Buenos Aires, Argentina. Se discute la posibilidad de noticiabilidad de esta información. Las entrevistas con los periodistas evidencian su falta de conocimiento científico y del patrimonio. Ambos aspectos generan una visión estereotipada del pasado que parece tener ninguna relación con el presente y, por lo tanto, justificar su exclusión en la agenda mediática. Sin embargo, hay una responsabilidad compartida entre los arqueólogos y los periodistas para encontrar nuevas e interesantes maneras de proporcionar información, así como transformar las debilidades en oportunidades. Tomando la falta general de conocimiento sobre el pasado como un reto para convertir la información científica en noticiable y promover un cambio para democratizar las competencias claves del aprendizaje y formar ciudadanos comprometidos y sensibles con sus contextos sociales y culturales.","palabras_clave":"Comunicación|Patrimonio arqueológico|Educación|Medios de comunicación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4022,"idioma_id":5,"titulo":"Entre el diseño y la implementación de una actividad de Geometría: El área de la pirámide recta","resumen":"En este trabajo presentamos el diseño de una tarea referida a Geometría con la finalidad de hacer emerger el concepto de área de la pirámide recta a partir de su desarrollo plano. La implementación tuvo lugar en un curso con alumnos del Profesorado de Física en la Universidad Nacional de San Juan (Argentina) durante el año académico 2014.Para diagramar y fundamentar la propuesta, usamos elementos de la Educación Matemática Crítica, Teoría de Situaciones Didácticas y Enfoque Ontosemiótico del Conocimiento e Instrucción Matemática. El propósito de esto es poner de relieve algunos elementos para delinear actividades matemáticas que puedan realizarse de manera diferente en la clase universitaria, alejándolas de los modelos tradicionales de enseñanza y estimulando a los estudiantes para que construyan su propio conocimiento. Para ello, mostramos los criterios de diseño que empleamos y el modo en que realizamos un análisis a priori y posteriori de la tarea antes mencionada.","palabras_clave":"PROPUESTA DIDACTICA|CRITERIOS DE DISEÑO DE TAREAS|RESOLUCION DE PROBLEMAS|AREA DE LA PIRAMIDE RECTA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4023,"idioma_id":4,"titulo":"FLOUR FROM PROSOPIS ALBA COTYLEDONS: A NATURAL SOURCE OF NUTRIENT AND BIOACTIVE PHYTOCHEMICALS","resumen":"The Prosopis alba seed is a waste material in the process to produce pod flour. To suggest a potential use ofthese seeds it is necessary to determine the nutritional, phytochemical and functional quality ofcotyledon flour from Prosopis alba. This flour showed high level of proteins (62%), low content of totalcarbohydrate and fat. Free polyphenol (1150 ± 20 mg GAE\/100 g flour) and carotenoids(10.55 ± 0.05 mg b-CE\/100 g flour) compounds were the dominant compounds. The main identified constituentsin the polyphenolic extracts were C- glycosyl flavones, including schaftoside, isoschaftoside,vicenin II, vitexin and isovitexin. The extract enriched in polyphenolic compounds exhibited ABTS+reducing capacity and scavenging activity of H2O2; and was able to inhibit phospholipase, lipoxygenaseand cyclooxygenase, three pro-inflammatory enzymes. According to our results, the P. alba cotyledonflour could be considered as a new alternative in the formulation of functional foods or food supplements.","palabras_clave":"PROSOPIS-ALBA|COTYLEDON-FLOUR|NUTRICIONAL-PROPERTIES|ANTIOXIDANT-ACTIVITY|ANTI-INFLAMMATORY-CAPACITY|GENOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4024,"idioma_id":4,"titulo":"Alumina-Supported Nickel onto Cordierite Monoliths for Ethane  Oxidehydrogenation: Coating Strategies and Their Effect on the  Catalytic Behavior","resumen":"Different strategies for the generation of Ni (15 wt %)\/Al2O3 catalytic coatings onto cordierite monoliths applied to the oxidative dehydrogenation of ethane (ODHE) were investigated. Strategy 1 involved the deposition of an alumina layer (nanoparticles or micro + nanoparticles) and the later incorporation of the active phase. Strategy 2 employed a suspension containing a powder catalyst that was used to deposit the catalytic film. Morphological (SEM) and physicochemical (LRS and XPS) characterizations were used to explain the catalytic performance of the structured systems. The NiO particles? distribution on the obtained catalytic coatings was analyzed via SEM-EDX performed in different monolith regions. The ultrasonic test was used to evaluate the anchoring of these catalytic films to the substrates. It was concluded that the selection of both the strategy and the particle size of the support is extremely important to obtain a homogeneously dispersed, accessible, and highly adhered to the substrate catalyst.","palabras_clave":"cordierite monoliths|coating strategies|alumina-supported nickel|ODH ethane"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4025,"idioma_id":4,"titulo":"NiCe\/\u0001-Al2O3 coated onto cordierite monoliths applied to Oxidative Dehydrogenation of Ethane (ODE)","resumen":"The present work investigates the preparation of NiCe\/g-Al2O3 coated cordierite monoliths for oxidative dehydrogenation of ethane (ODE) reaction through a two steps procedure: the primer deposition of a g-Al2O3 coating consisting in a mixture of micro and nanoparticles via the washcoating method and the latter incorporation of the active phase by impregnation. Moreover, a modification in these two steps was made: the suppression of some calcination steps to save energy in the manufacture process. The structured catalysts, named as Ni(X)Ce(Y)-M, where X =13 or 19 wt%. Ni and Y = 5 or 8 wt%. Ce respectively, with respect to the alumina loading, were characterized by SEM\/EDX, XRD, FTIR, LRS and XPS, and their catalytic activity was tested in the ODE reaction. Furthermore, their mechanical resistance to vibration was studied. Similar results corresponding to the unpromoted system are also presented for comparison. It was found that the co-impregnation of nickel and cerium was affected by the suppression of some calcinations: a preferential absorption of Ce rather than Ni occurred, generating at the end a catalytic coating with a higher Ce\/Ni ratio than that of the precursor solution and a lower Ni loading than that of Ni(13)-M. On the other side, in the NiCe-based catalysts the properties of the active sites remained the same despite the increment in the active phase, at a fixed Ce\/Ni nominal atomic ratio of 0.17. In the case of the Ni(19)Ce(8)-M system, although the Ni\/Al atomic ratios were found lower than those of the nominal ones by EDX and XPS and ethylene selectivity decayed, ethane conversion resulted markedly higher, leading to an improved ethylene yield.","palabras_clave":"Nickel oxide|Nickel-cerium oxides|Cordierite monoliths|Oxidative dehydrogenation of ethane"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4026,"idioma_id":4,"titulo":"Probiotic effect on calves infected with Salmonella Dublin: haematological parameters and serum biochemical profile","resumen":"The aim of this study was to evaluate the effect of a probiotic\/lactose inoculum on haematological and immunological parameters and renal and hepatic biochemical profiles before and during a Salmonella Dublin DSPV 595T challenge in young calves. Twenty eight calves, divided into a control and probiotic group were used. The probiotic group was supplemented with 100 g lactose\/calf\/d and 1010 cfu\/calf\/d of each strain of a probiotic inoculum composed of Lactobacillus casei DSPV318T, Lactobacillus salivarius DSPV315T and Pediococcus acidilactici DSPV006T throughout the experiment. The pathogen was administered on day 11 of the experiment, at an oral dose of 109 cfu\/animal (LD50). Aspartate aminotransferase (AST), gamma glutamyl transpeptidase (GGT), urea, red blood cells, haemoglobin, haematocrit, mean cell haemoglobin (MCH), mean corpuscular volume, mean corpuscular haemoglobin concentration (MCHC), white blood cells, lymphocytes, neutrophils, band neutrophils, monocytes, eosinophils, basophils and the neutrophils\/lymphocytes ratio were measured on days 1, 10, 20 and 27 of the experiment. In addition, animals were necropsied to evaluate immunoglobulin A (IgA) production in the jejunal mucosa. The most significant differences caused by the administration of the inoculum\/lactose were found during the acute phase of Salmonella challenge (9 days after challenge), when a difference between groups in neutrophils\/lymphocytes ratio were detected. These results suggest that the probiotic\/lactose inoculum administration increases the calf?s ability to respond to the disease increasing the systemic immune response specific. No differences were found in haemoglobin, haematocrit, MCH, MCHC, AST, urea, GGT, band neutrophils, eosinophils, monocytes and IgA in the jejunum between the two groups of calves under the experimental conditions of this study. Further studies must be conducted to evaluate different probiotic\/pathogens doses and different sampling times, to achieve a greater understanding of the effects of this inoculum on intestinal infections in young calves and of its mechanism of action.","palabras_clave":"PROBIOTIC|LACTIC ACID BACTERIA|IMMUNE SYSTEM|SALMONELLA SPP."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4027,"idioma_id":4,"titulo":"Fumagillin control of Nosema ceranae (Microsporidia: Nosematidae) infection in honey bee (Hymenoptera: Apidae) colonies in Argentina","resumen":"Information on the long-term consequences of Nosema.ceranae in honey bee lifespan and effectiveness of its control with fumagillin is scarce and varied. . Our objective was 10 to evaluate the effectiveness of the antibiotic fumagillin to control N.ceranae in hives in east-central Argentina. Honey bee hives were assigned to 3 different experimental treatments, a control group with un-treated hives, a preventive strategy group with hives treated monthly, and a monitoring strategy group with hives treated according to a N. ceranae level threshold. Apiaries were monitored monthly during fall-winter 2009 15 and 2010 and N. ceranae spore intensity and honey bee colony strength measures were estimated. Fumagillin-treated colonies hadreduced N. ceranae spores load in2010 compared to control colonies. However, there was no significant difference between treated and control groups for colonies strength measures including adult bee population, bee brood availability, honey or pollen.Fumagillin treatment reduced N. ceranae 20 intensities, but had little effect on colonies. The bee population during winter was reduced in treated as well as in control colonies. Our results clarify that fumagillin treatment should be at least reviewed and that further researc","palabras_clave":"ANTIBIOTIC CONTROL|APICULTURE|APIS MELLIFERA|FUMAGILIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4029,"idioma_id":4,"titulo":"Varroa destructor and Viruses association in honey bee colonies under different climatic  conditions","resumen":"Honey bee colonies are threatened by multiple factors including complex interactions between environmental and diseases such as parasitic mites and viruses. We compared the presence of honeybee-pathogenic viruses and Varroa infestation rate in four apiaries: commercial colonies that received treatment against Varroa and non-treated colonies that did not received any treatment for the last four years located in temperate and subtropical climate. In addition, we evaluated the effect of climate and Varroa treatment on DWV amounts. In both climates, DWV was the most prevalent virus, being the only present virus in subtropical colonies. Moreover, colonies from subtropical climate also showed reduced DWV amounts and lower Varroa infestation rates than colonies from temperate climate. Nevertheless, non-treated colonies in both climate conditions are able to survive several years. Environment appears as a key factor interacting with local bee populations and influencing colony survival beyond Varroa and Virus presence.","palabras_clave":"DEFORMED WING VIRUS|VARROA MITES|CLIMATE|STRESS TOLERANCE|HONEYBEE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4030,"idioma_id":4,"titulo":"A myiasis model for Philornis torquans (Diptera: Muscidae) and Pitangus sulphuratus (Passeriformes: Tyrannidae)","resumen":"The genus Philornis comprises neotropical parasitic flies that parasites bird nestlings in their larval stage. The ecology of most species of these parasitic flies is largely unknown. Here we present an epidemiological model that describes the behavior of parasite and host populations. The model was validated with real data of nestlings of the bird community present in a 30&nbsp;ha area in Santa Fe, Argentina. It consists of two weakly coupled population models, one for the larval population and the other for the nestling population. It takes into account, among other things, the importance of age structure for both populations, the immune response rate on the host and larval survivor rate, the incidence of larval load on host death rate, along with others. This work presents a simple and intuitive way to represent the behavior of this complex biological system and it is a good starting point for future studies.","palabras_clave":"MYIASIS|PARASITE|HOST|LARVA|MODEL|EPIDEMIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4032,"idioma_id":4,"titulo":"Protective role against hydrogen peroxide and fibroblast stimulation via Ce-doped TiO2 nanostructured materials","resumen":"Background: Cerium oxide (CeO2) and Ce-doped nanostructured materials (NMs) are being seen as innovativetherapeutic tools due to their exceptional antioxidant effects; nevertheless their bio-applications are still intheir infancy.Methods: TiO2, Ce?TiO2 and CeO2?TiO2 NMs were synthesized by a bottom-up microemulsion-mediatedstrategy and calcined during 7 h at 650 °C under air flux. The samples were compared to elucidate thephysicochemical characteristics that determine cellular uptake, toxicity and the influence of redox balancebetween the Ce3+\/Ce4+ on the cytoprotective role against an exogenous ROS source: H2O2. Fibroblasts wereselected as a cell model because of their participation in wound healing and fibrotic diseases.Results: Ce?TiO2 NM obtained via sol?gel reaction chemistry of metallic organic precursors exerts a realcytoprotective effect against H2O2 over fibroblast proliferation,while CeO2 pre-formed nanoparticles incorporatedto TiO2 crystalline matrix lead to a harmful CeO2?TiO2 material. TiO2 was processed by the same pathways asCe?TiO2 and CeO2?TiO2 NM but did not elicit any adverse or protective influence compared to controls.Conclusions: It was found that the Ce atoms source and its concentration have a clear effect on material´sphysicochemical properties and its subsequent influence in the cellular response. It can induce a range of biologicalreactions that vary from cytotoxic to cytoprotective.General significance: Even though there are still some unresolved issues and challenges, the unique physical andchemical properties of Ce-based NMs are fascinating and versatile resources for different biomedical applications.","palabras_clave":"REACTIVE_OXYGEN_SPECIES|NANOCERIA|FIBROBLASTS|CE-DOPED-TITANIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4033,"idioma_id":4,"titulo":"Prevalence and antifungal susceptibility of Candida albicans and its related species Candida dubliniensis and Candida africana  isolated from vulvovaginal samples in a hospital of Argentina","resumen":"Candida africana taxonomical status is controversial. It was proposed as a separatespecies within the Candida albicans species complex; however, phylogenetic analyses suggestedthat it is an unusual variety of C. albicans. The prevalence of C. albicans-related species(Candida dubliniensis and C. africana) as vulvovaginal pathogens is not known in Argentina.Moreover, data on antifungal susceptibility of isolates causing vulvovaginal candidiasis is scarce.The aims of this study were to establish the prevalence of C. dubliniensis and C. africana in vaginalsamples and to evaluate the antifungal susceptibilities of vaginal C. albicans species complexstrains. We used a molecular-based method coupled with a new pooled DNA extraction methodologyto differentiate C. dubliniensis and C. africana in a collection of 287 strains originallyidentified as C. albicans isolated from an Argentinian hospital during 2013. Antifungal susceptibilitiesto fluconazole, clotrimazole, itraconazole, voriconazole, nystatin, amphotericin B andterbinafine were evaluated by using the CLSI M27-A3 and M27-S4 documents. Of the 287 isolates,4 C. dubliniensis and one C. africana strains (1.39% and 0.35% prevalence, respectively) wereidentified. This is the first description of C. africana in Argentina and its identification was con-firmed by sequencing the ITS2 region and the hwp1 gene. C. dubliniensis and C. africana strainsshowed very low MIC values for all the tested antifungals. Fluconazole-reduced-susceptibilityand azole cross-resistance were observed in 3.55% and 1.41% of the C. albicans isolates, respectively.These results demonstrate that antifungal resistance is still a rare phenomenon in thiskind of isolates.","palabras_clave":"CANDIDA|TAXONOMICAL|PHYLOGENETIC|VULVOVAGINAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4034,"idioma_id":4,"titulo":"Development of Crystal Violet encapsulation in Pectin - Arabic gum gel microspheres","resumen":"Six pectins with 35% to 91% methoxylation degree were purified and characterized to encapsulate Crystal Violet (CV). Amidated low methoxylated pectin (ALMP) was selected based on microsphere morphologies, aqueous solubility, viscosity and the effect of calcium concentration. Pectin microspheres were stabilized with arabic gum (AG) and optimized according to the loading. Microspheres composed of 2.0% ALMP-1.0% AG crosslinked with 450 mM Calcium were able to encapsulate 217±2 &#956;M CV. Optical microscopy of the gels revealed spheroid microspheres with 250±50 &#956;m diameter containing homogenous CV distribution. Dried microspheres observed by SEM and epifluorescence showed a high shrinkable matrix keeping the spheroidal morphology. Low relative viscosity of the ALMP-AG-CV solutions was found compared to ALMP and ALMP-AG. The Young moduli (60-80 Pa) of ALMP-AG microspheres by texturometric analysis indicated that the CV could interfere with the gel crosslinking. Raman spectroscopy analysis suggested strong interaction of CV nucleophilic center within the matrix. FTIR of the matrix showed largest shifts in the carbonyl and carboxylate groups probably associated to H-bridges. CV stability studies performed on ALMP-AG microspheres, synthetized from polymer solutions with pH values above and below pectin pKa, showed faster CV release rate at the higher pH in presence of ionic strength.","palabras_clave":"CRYSTAL VIOLET|PECTIN |ARABIC GUM|MICROENCAPSULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4035,"idioma_id":4,"titulo":"Recovery of Interdependent Networks","resumen":"Recent network research has focused on the cascading failures in a system of interdependent networks and the necessary preconditions for system collapse. An important question that has not been addressed is how to repair a failing system before it suffers total breakdown. Here we introduce a recovery strategy for nodes and develop an analytic and numerical framework for studying the concurrent failure and recovery of a system of interdependent networks based on an efficient and practically reasonable strategy. Our strategy consists of repairing a fraction of failed nodes, with probability of recovery $gamma$, that are neighbors of the largest connected component of each constituent network. We find that, for a given initial failure of a fraction $1&#8201;&#8722;&#8201;p$ of nodes, there is a critical probability of recovery above which the cascade is halted and the system fully restores to its initial state and below which the system abruptly collapses. As a consequence we find in the plane $gamma &#8722;&#8201;p$ of the phase diagram three distinct phases. A phase in which the system never collapses without being restored, another phase in which the recovery strategy avoids the breakdown, and a phase in which even the repairing process cannot prevent system collapse.","palabras_clave":"RECOVERY|CASCADE OF FAILURES|COMPLEX NETWORKS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4036,"idioma_id":4,"titulo":"SONOGRAPHIC EVALUATION OF EPIDURAL AND INTRATHECAL INJECTIONS IN CATS","resumen":"Abstract Objectives To describe the ultrasonographic anat-omy of the caudal lumbar spine in cats and to detectultrasound (US) signs associated with epidural orintrathecal injection.Study design Prospective, clinical study.Animals Twenty-six client-owned cats.Methods Transverse (position 1) and parasagittal(position 2) two-dimensional US scanning wasperformed over the caudal lumbar spine in allcats. Midline distances between the identi&#64257;edstructures were measured. Cats assigned to epidu-ral injection (group E, n = 16) were administered abupivacaine?morphine combination con&#64257;rmed byelectrical stimulation. Cats assigned to intrathecalinjection (group I, n = 10) were administered amorphine?iohexol combination injected at thelumbosacral level and con&#64257;rmed by lateral radio-graphy. The total volume injected (0.3 mL kg&#57345;1)was divided into two equal aliquots that wereinjected without needle repositioning, with the USprobe in positions 1 and 2, respectively. Thepresence or absence of a burst of color [color &#64258;owDoppler test (CFDT)], dural sac collapse andepidural space enlargement were registered duringand after both injections.Results US scanning allowed measurement of thedistances between the highly visible structures insidethe spinal canal. CFDT was positive for all animals ingroup E. In group I, intrathecal injection wascon&#64257;rmed in only two animals, for which the CFDTwas negative; seven cats inadvertently and simulta-neously were administered an epidural injection andshowed a positive CFDT during the second aliquotinjection, and the remaining animal was adminis-tered epidural anesthesia and was excluded from theCFDT data analysis. Dural sac collapse and epiduralspace enlargement were present in all animals inwhich an epidural injection was con&#64257;rmed.Conclusions and clinical relevance US examinationallowed an anatomical description of the caudallumbar spine and real-time con&#64257;rmation of epiduralinjection by observation of a positive CFDT, dural saccollapse and epidural space enlargement.Keywords cats, doppler, epidural, intrathecal, ultra-sound.Intr","palabras_clave":"CATS|DOPPLER|EPIDURAL|INTRATHECAL|ULTRASOUND"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4037,"idioma_id":4,"titulo":"Expression and refolding of the protective antigen of Bacillus anthracis: A model for high-throughput screening of antigenic recombinant protein refolding","resumen":"Abstract Bacillus anthracis protective antigen (PA) is a well known and relevant immunogenicprotein that is the basis for both anthrax vaccines and diagnostic methods. Properly foldedantigenic PA is necessary for these applications. In this study a high level of PA was obtained inrecombinant Escherichia coli. The protein was initially accumulated in inclusion bodies, whichfacilitated its efficient purification by simple washing steps; however, it could not be recognizedby specific antibodies. Refolding conditions were subsequently analyzed in a high-throughputmanner that enabled nearly a hundred different conditions to be tested simultaneously. Therecovery of the ability of PA to be recognized by antibodies was screened by dot blot usinga coefficient that provided a measure of properly refolded protein levels with a high degreeof discrimination. The best refolding conditions resulted in a tenfold increase in the intensityof the dot blot compared to the control. The only refolding additive that consistently yieldedgood results was L-arginine. The statistical analysis identified both cooperative and negativeinteractions between the different refolding additives. The high-throughput approach describedin this study that enabled overproduction, purification and refolding of PA in a simple andstraightforward manner, can be potentially useful for the rapid screening of adequate refoldingconditions for other overexpressed antigenic proteins.","palabras_clave":"Bacillus anthracis|Protective antigen|Protein refolding|High-throughput refolding screening"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4038,"idioma_id":4,"titulo":"Selection of astrophysical\/astronomical\/solar sites at the Argentina East Andes range taking into account atmospheric components","resumen":"In the present work we analyze sites in the Argentinian high Andes mountains as possible places for astrophysical\/astronomical\/solar observatories. They are located at: San Antonio de los Cobres (SAC) and El Leoncito\/CASLEO region: sites 1 and 2. We consider the following atmospheric components that affect, in different and specific wavelength ranges, the detection of photons of astronomical\/astrophysical\/solar origin: ozone, microscopic particles, precipitable water and clouds. We also determined the atmospheric radiative transmittance in a day near the summer solstice at noon, in order to confirm the clearness of the sky in the proposed sites at SAC and El Leoncito. Consequently, all the collected and analyzed data in the present work, indicate that the proposed sites are very promising to host astrophysical\/astronomical\/solar observatories. Some atmospheric components, like aerosols, play a significant role in the attenuation of light (Cherencov and\/or fluorescence) detected in cosmic rays (particles or gamma photons) astrophysical observatories, while others, like ozone have to be considered in astronomical\/solar light detection.","palabras_clave":"Astrophysical|Astronomical|Solar|Sites|Argentina-Andes: atmospheric components"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4039,"idioma_id":4,"titulo":"-\tA description of three cases of parasitic Meningomyelitis in felines from the province of Santa Fe, Argentina.","resumen":"The present study reports the case of three female felines from rural areas, which were taken for consultation to the School of Veterinary Sciences of Universidad Nacional de Litoral (UNL). They presented severe chronic movement difficulties, mainly in their hindquarters. They underwent a semiological and neurologic exam and x-rays of the thoracolumbar spine (lateral and ventro-dorsal views). Blood and serum samples were taken for blood count and biochemistry. The x-ray films did not exhibit disorders in the spine and the blood count revealed eosinophilia. The three of them presented evident ambulatory ataxia and paresia. At necropsy, we could observe redness of the dura with a varicose subarachnoid hemorrhage at the lumbar level of the spine. Histopathologically speaking, meningomyelitis with thrombophlebitis at lumbar levels, a leukocyte exudate mostly containing eosinophils and the formation of perivascular cuffs were observed. Besides, parasitic forms containing eggs and calcified structures compatible with them were found. Considering the signs, the macroscopic and microscopic lesions and the origin of the animals, it is believed that they were infested by Gurltia paralysans.","palabras_clave":"FELINES|MENINGOMYELITIS|NEMATODES|GURLTIA PARALYSANS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4040,"idioma_id":4,"titulo":"Concentration of trace elements in raw milk from cows in the southeast of Cordoba province, Argentina","resumen":"In recent years, trace elements in cow milk have been considered goodbioindicators of pollution in the agricultural environment. The aim of the study was to analyze the correlation between trace element content in livestock drinking water and cow milk from dairy farms located in the southeast of Córdoba province, Argentina.Groundwater is the main source of livestock drinking water. According to the results, trace elements were grouped in three categories: (a) those that were in high concentration in phreatic water and in low concentration in deep wells (As, V); (b) those which showed the opposite trend (Cu, Fe, Mn, Zn); and (c) those that were in very low concentrations in all water samples (Cd, Cr, Ni, Pb, and Se). In elements from group (a), a positive correlation between As content in water and in milk was observed. For elements included in group (b), it was observed a higher concentration in milk samples from farms that use deep wells, related with their higher concentrations in water. Cr, Ni, Pb, and Se milk contents are within the ranges reported in other areas. Soil and foragetrace element content may contribute to their presence in milk. Since information about transference of trace elements from environmental matrices to milk is very scarce, at national and international levels, further studies are necessary, including speciation in milk and dairy products, to guarantee food safety","palabras_clave":"Cow milk|Trace elements|Groundwater|Soil and forage"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4041,"idioma_id":5,"titulo":"Fisiopatología del Hiperadrenocorticismo en felinos. Presentación de caso clínico","resumen":"El hiperadrenocorticismo es una endocrinopatía poco frecuente en felinos que se caracteriza por niveles elevados de cortisol en sangre, lo que genera la presentación de numerosos signos clínicos entre los que se destacan hiperglucemias moderadas, persistentes y sostenidas en el tiempo, poliuria, polidipsia, polifagia y dermatopatías. La edad promedio de presentación se encuentra alrededor de los 10 años. Esta enfermedad suele presentarse en combinación con otras endocrinopatías como la diabetes mellitus. La enfermedad se puede producir por afección primaria de la hipófisis o de las glándulas adrenales. Se presenta un caso clínico con su diagnóstico diferencial y revisión bibliográfica del tema. ","palabras_clave":"HIPERADRENOCORTICISMO|SINDROME DE CUSHING|HIPERGLUCEMIA|POLIDIPSIA|POLIURIA|POLIFAGIA|DIABETES MELLITUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4042,"idioma_id":5,"titulo":"Viral  diagnostic  criteria  for  Feline  immunodeficiency virus  and  Feline  leukemia  virus  infections  in  domestic cats  from  Buenos  Aires,  Argentina","resumen":"A cross-sectional study was carried out on cats attending the Small Animal Hospitalat the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalenceand associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus(FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptomscompatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed byserology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assaysshowed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV.Differences between the diagnostic tests and their choice according to the age of the animalare discussed. The clinical histories of ninety of the 255 cats showed blood profiles similarto others previously reported and revealed a higher risk of infection in male adult cats withoutdoor access.","palabras_clave":"Inmunodeficiencia felina|Leucemia felina|inmunocromatografia|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4043,"idioma_id":4,"titulo":"Upgrading of biomass-derived 2-hexanol to liquid transportation fuels on Cu-Mg-Al mixed oxides. Effect of Cu content","resumen":"The gas-phase synthesis of high molecular weight compounds of application as liquid transportation fuels from 2-hexanol was studied on Cu?Mg?Al mixed oxides with different copper content (0.3?61.2%) and a Mg\/Al = 1.5 M ratio. Catalysts were prepared by coprecipitation and characterized by severaltechniques such as BET surface area, XRD, TPD of CO2, TPR and N2O decomposition. Yields of up to 87% were obtained for compounds in the C9?C24 range, 80% of which were suitable as jet fuels and the rest as diesel substitutes. This product pool was a hydrophobic mixture of ketones, alcohols and hydrocarbons with 160?200 g\/mol average molecular weight and an O\/C atomic ratio as low as 0.04. Because low copper content catalysts are hard to reduce, on these materials the reaction occurs via a base-catalyzed mechanism involving consecutive dehydrogenation, CAC bond formation, dehydration and hydrogenation steps, that forms mainly even carbon atom number products. Partially reduced Cun+ atoms contribute to promote a distinct pathway toward odd products. In contrast, on high copper content oxides the reaction yields similar amounts of even and odd products and proceeds by a bifunctional Cu0-base mechanism in which the CAC coupling is rate-limiting.","palabras_clave":"jetfuels|biomass|2-hexanol|Cu catalysts"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4044,"idioma_id":4,"titulo":"Conversion of biomass-derived 2-hexanol to liquid transportation fuels: Study of the reaction mechanism on Cu-Mg-Al mixed oxides","resumen":"The reaction mechanism of 2-hexanol conversion to high molecular weight compounds to be used as liquid transportation fuels was studied on MgO, Cu\/SiO2 and a bifunctional Cu-Mg-Al mixed oxide with 8 wt. % Cu (catalyst 8.0CuMgAl). Catalysts were characterized by several physical and spectroscopic techniques. The evolution of 2-hexanol conversion and yields in inert (N2) and reducing (H2) reaction atmospheres at different contact times (W\/F0) was investigated, which allowed distinguishing between primary and secondary products. In H2, at W\/F0 = 500 g h\/mol, the bifunctional 8.0CuMgAl catalyst yielded more than 90 % of branched C9-C24 oxygenates and hydrocarbons that were obtained via sequential steps comprising dehydrogenation, C-C coupling, dehydration and hydrogenation reactions. The metal-base bifunctional nature of this reaction network on 8.0CuMgAl was elucidated: nano-sized Cu0 particles promote dehydrogenation and hydrogenation steps whereas Mg-O pairs participate mainly in C-C coupling reactions. The product distribution depended on the reaction atmosphere. In H2, the reaction pathways leading to formation of even carbon atom number products (C12, C18 and C24) were favored and hydrocarbons were the main products at high conversion levels. In N2, significant amounts of odd carbon atom number products (C9, C15 and C21) were formed with a higher contribution of oxygenates to the product pool.","palabras_clave":"Biomass valorisation|Liquid fuels|2-hexanol"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4045,"idioma_id":4,"titulo":"METHYLENE BLUE PREVENTS RETINAL DAMAGE IN AN EXPERIMENTAL MODEL OF ISCHEMIC PROLIFERATIVE RETINOPATHY","resumen":"Perinatal asphyxia induces retinal lesions, generating ischemic proliferative retinopathy, which may result in blindness. Previously, we showed that the nitrergic system was involved in the physiopathology of perinatal asphyxia. Here we analyze the application of methylene blue, a well-known soluble guanylate cyclase inhibitor, as a therapeutic strategy to prevent retinopathy. Male rats (n = 28 per group) were treated in different ways: 1) control group comprised born-to-term animals; 2) methylene blue group comprised animals born from pregnant rats treated with methylene blue (2 mg\/kg) 30 and 5 min before delivery; 3) perinatal asphyxia (PA) group comprised rats exposed to perinatal asphyxia (20 min at 37°C); and 4) methylene blue-PA group comprised animals born from pregnant rats treated with methylene blue (2 mg\/kg) 30 and 5 min before delivery, and then the pups were subjected to PA as above. For molecular studies, mRNA was obtained at different times after asphyxia, and tissue was collected at 30 days for morphological and biochemical analysis. Perinatal asphyxia produced significant gliosis, angiogenesis, and thickening of the inner retina. Methylene blue treatment reduced these parameters. Perinatal asphyxia resulted in a significant elevation of the nitrergic system as shown by NO synthase (NOS) activity assays, Western blotting, and (immuno)histochemistry for the neuronal isoform of NOS and NADPH-diaphorase activity. All these parameters were also normalized by the treatment. In addition, methylene blue induced the upregulation of the anti-angiogenic peptide, pigment epithelium-derived factor. Application of methylene blue reduced morphological and biochemical parameters of retinopathy. This finding suggests the use of methylene blue as a new treatment to prevent or decrease retinal damage in the context of ischemic proliferative retinopathy.","palabras_clave":"ANGIOGENESIS|METHYLENE BLUE|ISCHEMIC PROLIFERATIVE RETINOPATHY|NITRIC OXIDE|RETINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4046,"idioma_id":4,"titulo":"Identification of nurseries areas of Juvenile Prochilodus lienatus (Valenciennes, 1836) by scale and otolith morphometry and microchemistry","resumen":"The streakedprochilod (Prochilodus lineatus Valenciennes, 1836) is acommercially freshwater species from South America, distributed in the PlataBasin. In the present work the morphometry (circularity, rectangularity, formfactor, OL\/OW and ellipticity indices) and chemistry (Sr:Ca, Ba:Ca, Mg:Ca)of&nbsp;lapilli&nbsp;otolith, and geometric morphometry of scales of streaked prochilod juveniles, in two sites in the Plata basin(Uruguay River and Estrella Wetland), were compared to determine if they arearea-specific and to identify possible breeding areas. Otolith Ba:Ca ratios was0.017±0.003 mmol\/mol for Uruguay River while was not above the detection limitsfor Estrella&nbsp;Wetland&nbsp;individuals. Zn:Ca ratios tended to be higherfor latter&nbsp;(0.03±0.002 mmol\/mol). Significantly high circularity and lowrectangularity values were obtained for the Estrella&nbsp;Wetland&nbsp;otoliths(p &lt; 0.05), while no significant differences for form factor, OL\/OW andellipticity were observed between sampling sites.&nbsp;Considering all scalegeometric morphometry&nbsp;variables, discriminant analysis showed a goodpercentage of classification of individuals (90.5% forEstrella&nbsp;Wetland&nbsp;and 85.7 % for Uruguay River).&nbsp;These resultsindicate that the otolith microchemisty and morfometry (circularity andrectangularity indices) and&nbsp;scale morphometry&nbsp;are good markersof habitat and&nbsp;represent a potential tool for identification of streaked prochilod nursery areas.","palabras_clave":"streaked prochilod,|nursery areas|otolith, |scale, morphometry and microchemistry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4047,"idioma_id":4,"titulo":"Role of the capping agent in the interaction of hydrophilic Ag nanoparticles with DMPC as a model biomembrane","resumen":"The interaction of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) Langmuir monolayers as a model biomembrane with small silver nanoparticles (AgNPs) similar in size but coated with different capping molecules such as citrate (CIT-AgNPs) and 4-mercaptobenzoic acid (MBA-AgNPs), both negatively charged at physiological pH, is studied using a multi-technique approach. Both CIT-AgNPs and MBA-AgNPs expose carboxyl groups and have similar zeta potentials, but differ in the aliphatic or aromatic nature of the capping agent. Results show that AgNPs exhibit quite different behaviors: CIT-AgNPs weakly adsorb on DMPC, while MBA-AgNPs irreversibly adsorb on the interface and remain there upon monolayer compression. It is also shown that there is a cooperative effect of many ligands in the interactions between MBA-AgNPs and DMPC, as MBA molecules in solution are unable to strongly adsorb on the phospholipid monolayer surface. We propose an explanation based on the surface charge density and on the chemical nature of the capping molecule based on XPS studies and on DFT calculations.","palabras_clave":"SILVER NANOPARTICLES|DMPC|NANOSCIENCE|INTERFACES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4048,"idioma_id":4,"titulo":"Open Source Quartz Crystal Microbalance with dissipation monitoring","resumen":"The dissipation factor and subsequently the characterization of the viscoelasticity ofdeposition films have become crucial for the study of biomolecular adsorption. Most of thecommercial quartz crystal microbalance (QCM) systems offer this feature, but it has not beenincorporated in open source systems. This article describes the design, construction, andsimulation of an open source QCM module for measuring dissipation factor. The moduleincludes two blocks: switch and envelope detector. The switch rapidly disrupts the excitationof the crystal, and connects the output to the envelope detector which demodulates theamplitude of the signal. Damped sinusoidal exponential signals with different time constantwere used for simulating viscosity interfaces. The incorporation of few elements facilitated adouble-faced PCB design with reduced dimensions. The results from simulation show that thesystem has a good performance in the range of the biomolecular processes; greater relativeerror are observed for time constant lower than 1 us. In conclusion, a dissipation module hasbeen developed for calculate dissipation factor using QCM, which is compact and shows greatperformance for use in biomolecular adsorption.","palabras_clave":"DISSIPATION|QCM|VISCOELASTIC|ENVELOPE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4049,"idioma_id":4,"titulo":"Vesicles cytoplasmic injection: an efficient technique to produce porcine transgene expressing embryos","resumen":"The use of vesicles co-incubated with plasmids showed to improve the efficiency of cytoplasmic injection of transgenes in cattle. Here this technique was tested as a simplified alternative for transgenes delivery in porcine zygotes. To this aim, cytoplasmic injection of the plasmid alone was compared to the injection with plasmids co-incubated with vesicles both in diploid parthenogenic and IVF zygotes. The plasmid pcx-egfp was injected circular (CP) at 3, 30 and 300ng\/mL and linear (LP) at 30ng\/&#956;L. The experimental groups using parthenogenetic zygotes were: CP naked at 3 ng\/mL (N=105), 30ng\/mL (N=95) and 300ng\/mL (N=65); Sham (N=105); control not injected (N=223); LP naked at 30ng\/mL (N=78); LP vesicles (N=115) and Sham vesicles (N=59). For IVF zygotes: LP naked (N=44) LP vesicles (N=94), sham (N=59) and control (N=79). Cleavage, blastocyst and GFP+ rates were analyzed by Fisher?s test (P<0.05). The parthenogenic CP naked group showed lower cleavage respect to control (p<0.05). The highest concentration of plasmids to allow development to blastocyst stage was 30ng\/mL. There were no differences in DNA fragmentation between groups. The parthenogenic LP naked group resulted in high GFP rates (46%) and also allowed the production of GFP-blastocysts (33%). The cytoplasmic injection with LP vesicles into parthenogenic zygotes allowed 100% GFP-blastocysts. Injected IVF showed higher cleavage rates than control (p<0.05). In IVF zygotes only the use of vesicles produced GFP-blastocysts. The use of vesicles co-incubated with plasmids improves the transgene expression efficiency for cytoplasmic injection in porcine zygotes andconstitutes a simple technique for easy delivery of plasmids.","palabras_clave":"PORCINOS|TRANSGENESIS|PARTENOGÉNESIS|FIV"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4050,"idioma_id":5,"titulo":"Variabilidad germinativa en semillas de diferentes poblaciones de cuatro especies de gramineas nativas de Patagonia: un estudio de caso en el noroeste de Chubut","resumen":"En este estudio se analizó la calidad y el comportamiento germinativo de semillas de diferentes poblaciones localizadas en un gradiente de precipitación anual oeste-este (500 a 150 mm) en cuatro especies de gramíneas forrajeras nativas (Bromus setifolius, Hordeum comosum, Festuca pallescens y Poa ligularis), del NO de Chubut. Se estimó el peso y el porcentaje de semillas llenas a&nbsp; nivel&nbsp; inter-&nbsp; e&nbsp; intra-específico.&nbsp; Las&nbsp; semillas&nbsp; fueron&nbsp; sembradas&nbsp; en&nbsp; un&nbsp; ambiente&nbsp; común&nbsp; y&nbsp; se&nbsp; calculó el porcentaje de germinación y la velocidad de emergencia para las especies y las poblaciones. En este trabajo se presentan datos relacionados a la germinación de semillas recolectadas en un año en particular (2015) de estas cuatro especies y se discuten principalmente los resultados obtenidos para P. ligularis, la especie que presentó mayor variabilidad entre las poblaciones analizadas. En P. ligularis un gran porcentaje de semillas de la población del sitio más árido germinaron\/emergieron en menos días. Esta característica podría estar asociada a adaptaciones fisiológicas de las plantas para germinar, emerger y establecerse rápidamente ante la escasa disponibilidad de agua en este sitio. Los genotipos de esta población particular podrían ser utilizados para programas de restauración de ambientes áridos con precipitación media anual menor a 150 mm. Finalmente, debido a la variabilidad observada al menos en P. ligularis, se sugiere que para futuros programas de mejoramiento con estas especies nativas sería necesario analizar un mayor número de poblaciones para seleccionar los potenciales bancos de semilla.&nbsp;","palabras_clave":"Bromus setifolius|ecotypes|Gestuca pallescens|germination"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4051,"idioma_id":4,"titulo":"Influence of Ultrasound Speckle Tracking Strategies for Motion and Strain Estimation","resumen":"Speckle Tracking is one of the most prominent techniques used to estimate the regional movement of the heart based on ultra- sound acquisitions. Many different approaches have been proposed, proving their suitability to obtain quantitative and qualitative information regarding myocardial deformation, motion and function assessment. New proposals to improve the basic algorithm usually focus on one of these three steps: (1) the similarity measure between images and the speckle model; (2) the transformation model, i.e. the type of motion considered between images; (3) the optimization strategies, such as the use of different optimization techniques in the transformation step or the inclusion of structural information. While many contributions have shown their good performance independently, it is not always clear how they perform when integrated in a whole pipeline. Every step will have a degree of influence over the following and hence over the final result. Thus, a Speckle Tracking pipeline must be analyzed as a whole when developing novel methods, since improvements in a particular step might be undermined by the choices taken in further steps. This work presents two main contributions: (1) We provide a complete analysis of the influence of the different steps in a Speckle Tracking pipeline over the motion and strain estimation accuracy. (2) The study proposes a methodology for the analysis of Speckle Tracking systems specifically designed to provide an easy and systematic way to include other strategies. We close the analysis with some conclusions and recommendations that can be used as an orientation of the degree of influence of the models for speckle, the transformation models, interpolation schemes and optimization strategies over the estimation of motion features. They can be further use to evaluate and design new strategy into a Speckle Tracking system.","palabras_clave":"Speckle Tracking|Strain Estimation|Maximum Likelihood|Diffeomorphic Registration"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4052,"idioma_id":4,"titulo":"Assessment of Estradiol Response after Depot Triptorelin Administration in Girls  with Central Precocious Puberty.","resumen":"BACKGROUND:Estradiol at baseline or after a classical gonadotropin-releasing hormone test did not reflect ovarian steroidogenesis in central precocious puberty (CPP) girls.AIMS:To evaluate estradiol response to depot triptorelin, both at start and during therapy to determine how active ovarian steroidogenesis is at pubertal stage and under therapy.METHODS:A prospective study was performed in 43 CPP girls. Serum luteinizing hormone and follicle-stimulating hormone at 3 h (LH-3h, FSH-3h) and estradiol at 24 h (E2-24h) after injection of depot triptorelin 3.75 mg were measured, at first dose and at 3, 6, 12, 18 and 24 months of treatment.RESULTS:E2-24h after depot triptorelin was >100 pg\/ml after the first dose. Estradiol response (E2-24h) fell to levels <14 pg\/ml in 78 out of 82 follow-up visits along 2 years of therapy. Concomitantly, LH-3h and FSH-3h were <4.0 and <6.3 IU\/l, respectively. In 4 patients with inadequate treatment, E2-24h, LH-3h and FSH-3h rose to pubertal values similar to those observed at first dose.CONCLUSION:Estradiol (<14 pg\/ml) assessment 24 h after depot triptorelin administration is a reliable and simple manner to confirm ovarian suppression in CPP girls during treatment.","palabras_clave":"Triptorelin|Precocious puberty|depot admisnistration|estradiol response"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4053,"idioma_id":4,"titulo":"Composites based on modified clay assembled Rh(III) heteropolymolybdates as catalysts in the liquid-phase hydrogenation of cinnamaldehyde","resumen":"New composites based on the [RhMo6O24H6]3&#8722; (RhMo6) heteropolyanion supported on pillared (PILC), heterostructured (PCH) and functionalized(PILC-F) and (PCH-F) systems based on clays were prepared, characterized and tested as catalysts in the liquid-phase hydrogenation of cinnamaldehyde. The original phases and supported systems were characterized using several techniques such as powder X-ray diffraction (XRD), scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM?EDS), Raman microprobe studies, X-ray photoelectron spectroscopy (XPS), thermogravimetry and differential thermal analyses (TG-DSC), temperature programmed reduction (TPR), and textural analysis (BET method), which confirmed their functionalization, physicochemical modification and the nature of Mo adsorbed species. Active acidic, basic and redox sites were determined by temperature programmed surface reaction (TPSR). Mo loading reached 7 wt% for the system RhMo6\/PCH-F and 3 wt% for the system RhMo6\/PILC-F, while unfunctionalized clay systems showed a value of 1 wt% of Mo. The catalytic performance showed that PCH-based composites were the most active and reached up to 56% conversion at 360 min of reaction when tested in liquid-phase cinnamaldehyde hydrogenation. The selectivity for all the systems was mainly toward hydrocinnamic aldehyde (HCAL) and reached 77% for the RhMo6\/PCH-F catalyst at 25% conversion.","palabras_clave":"Rh(III)-heteropolymolybdates|Clays|Composites|Chemical modification|Liquid-phase hydrogenation|Cinnamaldehyde"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4054,"idioma_id":4,"titulo":"Reducing the capacitance of piezoelectric film sensors","resumen":"We present a novel design for large area, wideband, polymer piezoelectric sensor with low capacitance. The large area allows better spatial resolution in applications such as photoacoustic tomography and the reduced capacitance eases the design of fast transimpedance amplifiers. The metalized piezoelectric polymer thin film is segmented into N sections, electrically connected in series. In this way, the total capacitance is reduced by a factor (1\/N)^2, whereas the mechanical response and the active area of the sensor are not modified. We show the construction details for a two-section sensor,together with the impedance spectroscopy and impulse response experimental results that validate the design.","palabras_clave":"Capacitance|Amplifiers|Piezoelectric films|Vibration resonance|Capacitors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4055,"idioma_id":4,"titulo":"Notes on the distribution of the genus Andalgalomys (Rodentia, Cricetidae), with the first record for A. pearsoni (Myers, 1978) in Argentina","resumen":"Wedocumented the first record of Andalgalomys pearsoni (Myers 1978) inArgentina based on one individual caught at Cerro Colorado (Metán department,Salta province, Argentina). This new record enlarges the distribution of thisspecies, to date only known from Bolivia and Paraguay, ca. 530 km SW and fillsa gap without records for Andalgalomys of approximately 850 km between Paraguayand NW Argentina. The finding of this mouse, caught more than 50 years ago,highlights the importance that biological collections have in our understandingof biological diversity.","palabras_clave":"Cricetidae|Andalgalomys pearsoni|Argentina|Distribución"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4056,"idioma_id":4,"titulo":"Chitosan-starch beads prepared by ionotropic gelation as potential matrices for controlled release of fertilizers","resumen":"The present study examines the agrochemical application of macrospheres prepared with chitosan and chitosan-starch blends by an easy dripping technique, using a sodium tripolyphosphate aqueous solution as the crosslinking agent. These biopolymers form hydrogels that could be a viable alternative method to obtain controlled-release fertilizers (CRFs). Three different concentrations (ranging from 20 to 100 wt\/wt % of chitosan) and two crosslinking times (2 or 4 hours) were used. The resulting polymeric matrices were examined by scanning electron microscopy coupled with energy dispersive X-ray, X-ray diffraction, Fourier transform infrared spectroscopy, solid-state nuclear magnetic resonance, thermogravimetric analysis and differential scanning calorimetry. Ionotropic gelation and neutralization induced the formation of the macrospheres. The crosslinking time and the composition of the polymeric hydrogel controlled the crosslinking degree, the swelling behavior and the fertilizer loading capability. Potassium nitrate-loaded beads were shown to be useful as a controlled-release fertilizer. After 14 days of continuous release into distilled water, the cumulative concentration in the release medium reached between 70 and 93% of the initially loaded salt, depending on the matrix used. The prepared beads showed properties that make them suitable for use in the agrochemical industry as CRFs.","palabras_clave":"Chitosan|starch|macrospheres|fertilizer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4057,"idioma_id":4,"titulo":"A Study of Radio Polarization in Protostellar Jets","resumen":"Synchrotron radiation is commonly observed in connection with shocks of different velocities, ranging from relativistic shocks associated with active galactic nuclei, gamma-ray bursts, or microquasars, to weakly or non-relativistic flows such as those observed in supernova remnants. Recent observations of synchrotron emission in protostellar jets are important not only because they extend the range over which the acceleration process works, but also because they allow us to determine the jet and\/or interstellar magnetic field structure, thus giving insights into the jet ejection and collimation mechanisms. In this paper, we compute for the first time polarized (synchrotron) and non-polarized (thermal X-ray) synthetic emission maps from axisymmetrical simulations of magnetized protostellar jets. We consider models with different jet velocities and variability, as well as a toroidal or helical magnetic field. Our simulations show that variable, low-density jets with velocities of ?1000 km s-1 and ?10 times lighter than the environment can produce internal knots with significant synchrotron emission and thermal X-rays in the shocked region of the leading bow shock moving in a dense medium. While models with a purely toroidal magnetic field show a very large degree of polarization, models with a helical magnetic field show lower values and a decrease of the degree of polarization, in agreement with observations of protostellar jets.","palabras_clave":"HerbigHaro objects|ISM: jets and outflows|magnetohydrodynamics: MHD|polarization|radiation mechanisms: non-thermal| shock waves"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4058,"idioma_id":4,"titulo":"Baking of sponge cake: experimental characterization and mathematical modelling","resumen":"Sponge cake is a sweet bakery product that begins as a fluid batter and, during baking, transforms into a porous solid, presenting an important volume expansion. The aim of this work was, first of all, to study experimentally the influence of operative conditions (natural and forced convection; oven temperature, from 140 to 180 °C; steam addition) on volume expansion and the heat transfer dynamics during baking of sponge cake. It was observed that an increase in oven temperature, airflow and steam injection produces an increase in volume expansion. Secondly, a mathematical model was developed to simulate heat transfer coupled with volume expansion. Both experimental and simulated temperature profiles verified that the last region to achieve a correct degree of baking is the one near the crust around the axial axis. In consequence, the minimal baking time was defined as the average time at which this region reaches 95?98 °C. The baking time was strongly affected by the effective oven temperature, with a slight influence of the convection mode.","palabras_clave":"sponge cake|volume expansion|heat transfer simulation|baking time"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4059,"idioma_id":4,"titulo":"Autothermal reverse-flow reactors: design and comparison of valve-operated and rotary systems","resumen":"The valve-operated reverse-flow catalytic reactoris an efficient system for the treatment of air streams contaminated with smallamounts of volatile organic compounds (VOCs). Nonetheless, it has two drawbackswhen operated at low cycle periods: the emission of the volume withouttreatment present in the region close to the reactor input and an unavoidable reductionof the valve lifetime. Upon consideration that the use of shorter cycle periodswould enable the design of more compact units, we investigated the option ofoperating with reverse flow by means of a rotary catalytic reactor. Thisalternative eliminates the necessity of valves for the flow reversal and enablesto allot a small fraction of the total cross section to be fed with clean air andprevent the VOCs that remain close to the reactor input from being dischargedto the atmosphere.The aim of this work is to analyse thetreatment of an air stream contaminated with ethanol and ethyl acetate by meansof mathematical simulation of reverse-flow operations in both valve-operated androtary catalytic reactors. To that end, monolithic structures with squarechannels are assumed for both types of reverse-flow reactors, a design strategyis proposed and the results for both types of reactors are compared. It isconcluded that the rotary reverse-flow reactor arises as a better option.","palabras_clave":"VOLATILE ORGANIC COMPOUNDS|CATALYTIC COMBUSTION|VALVE-OPERATED REVERSE-FLOW REACTOR|ROTARY REVERSE-FLOW REACTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4060,"idioma_id":4,"titulo":"Kinetics of Pseudomonas veronii  2E biofilm development under different nutritional conditions for a proper bioreactor design","resumen":"Biofilm-mediated bioreactors represent a proficient alternative, as extracellular    materials of bacterial biofilms enhance metal immobilization by biosorption. Bacterial     attachment or detachment is related to environmental conditions. The aim of this work     is to study the nutritional dependence of Pseudomonas veronii 2E biofilm development     to improve a biofilm-reactor for wastewater treatments. For such purpose, biofilm     establishment kinetics was explored over glass coverslips changing medium    composition and nutrient concentration and in Fe(II) presence. After crystal violet     staining, biofilm was visualized using direct microscopic observation; sample scanning-  image analysis implemented in MATLAB and ethanol extraction-Absorbance 590nm     measurement. Biofilm structure developed with mineral basal medium appeared as a     monolayer, but in complex basal media cell aggregates were appreciated. Optimal    attachment was achieved with complex basal medium-5g\/L glucose at 55h. The crystal     violet concentration complemented with the image capturing supported these results.    The biofilm structure defined under such conditions is useful for the bioreactor     implementation.","palabras_clave":"wastewater biotreatment|metal bioremediation|biofilm reactor|Pseudomonas veronii"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4061,"idioma_id":4,"titulo":"Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform","resumen":"In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNP) onto vesicles bilayer with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology of self-assembled AuNP onto large unilamellar vesicles structures is described. The vesicles were formed with the 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNP photochemically synthesized in pure glycerol were mixed and anchored onto SH-DOPC vesicles.The data provided by voltammetry, spectrometry and microscopy techniques have indicated that the AuNP were successfully covalently anchored onto the vesicles bilayer and decorated vesicles present a spherical shape and size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using friendly-environmental procedures.","palabras_clave":"covalently decorated vesicles|gold nanoparticles|immunosensors desing|synthesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4062,"idioma_id":4,"titulo":"Square Wave Voltammetry: An Alternative Technique to Determinate Piroxicam Release Profile from Nanostructured Lipid Carriers","resumen":"A new, simple, and fast electrochemical (EC) method has beendeveloped to determine the release profile of piroxicam, a nonsteroidalanti-inflammatory drug, loaded in a drug deliverysystem based on nanostructured lipid carriers (NLCs). For thefirst time, the samples were analyzed by using square wavevoltammetry, a sensitive EC technique. The piroxicam EC responsesallow us to propose a model that explains the experimentalresults and to subsequently determine the amount ofdrug loaded into the NLCs formulation as a function of time. Invitro drug release studies showed prolonged drug release (upto 5 days), releasing 60% of the incorporated drug. The proposedmethod is a promising and stable alternative for thestudy of different drug delivery systems","palabras_clave":"Nanostructured Lipid Carrier |Release profile|Piroxicam|Square wave voltammetry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4063,"idioma_id":4,"titulo":"Effect of spermatozoa motility hyperactivation factors and gamete coincubation duration on in vitro bovine embryo development using flow cytometrically sorted spermatozoa","resumen":"The aim of the present study was to evaluate the effects of sperm motility enhancers and different IVF times on cleavage, polyspermy, blastocyst formation, embryo quality and hatching ability. In Experiment 1, sex-sorted X chromosome-bearing Bos taurus spermatozoa were incubated for 30 min before 18 h fertilisation with hyperactivating factors, namely 10 mM caffeine (CA), 5 mM theophylline (TH), 10 mM caffeine and 5 mM theophylline (CA + TH); and untreated spermatozoa (control). In Experiment 2, matured B. taurus oocytes were fertilised using a short (8 h) or standard (18 h) fertilisation length, comparing two different fertilisation media, namely synthetic oviducal fluid (SOF) fertilisation medium (SOF-FERT) and M199 fertilisation medium (M199-FERT). Cleavage and blastocyst formation rates were significantly higher in the CA + TH group (77% and 27%, respectively) compared with the control group (71% and 21%, respectively). Cleavage rates and blastocyst formation were significantly lower for the shortest fertilisation time (8 h) in M199-FERT medium (42% and 12%, respectively). The SOF-FERT medium with an 8 h fertilisation time resulted in the highest cleavage rates and blastocyst formation (74% and 29%, respectively). The SOF-FERT medium produced the highest embryo quality (50% Grade 1) and hatching rate (66%). Motility enhancers did not affect polyspermy rates, whereas polyspermy was affected when fertilisation length was extended from 8 h (3%) to 18 h (9%) and in M199-FERT (14%) compared with SOF-FERT (6%). We conclude that adding the motility enhancers CA and TH to sex sorted spermatozoa and Tyrode?s albumin lactate pyruvate (TALP)-Sperm can improve cleavage and embryo development rates without increasing polyspermy. In addition, shortening the oocyte-sperm coincubation time (8 h) resulted in similar overall embryo performance rates compared with the prolonged (18 h) interval.","palabras_clave":"IN VITRO FERTILIZATION|SEXED SEMEN|SPERMATOZOA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4064,"idioma_id":4,"titulo":"Hypostomus formosae, a new catfish species from the Paraguay River Basin with redescription of H. boulengeri (Siluriformes: Loricariidae)","resumen":"Hypostomus formosae n. sp. is described for the Paraguay river basin, Argentina. It can be distinguished from all other Hypostomus by having the combination of the following features: dorsum of head, body and all fins bright grey covered by numerous rounded dark dots and 11 ventral plates between end of anal-fin base and caudal fin (vs. more than 11). In addition, the new species is distinguished from the most similar sympatric species, H. boulengeri by slightly higher cleithral width, dorsal-fin base length, caudal-peduncle depth and by narrower caudal-peduncle length, eye diameter and mandibulary ramus length. Hypostomus formosae belongs to the Amazonian ?H. plecostomus species group? and our molecular phylogenetic results show that it is the sister species to the Guianese H. plecostomus, highlighting past inter-basin ichtyofauna exchanges. We also provide detailed record of H. boulengeri for the first time in Argentina, collected in Paraguay river, at Formosa city.","palabras_clave":"Neotropical |Fishes|Hypostomus|mitonchondrial DNA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4065,"idioma_id":4,"titulo":"Multidisciplinary study of chemical and biological factors related to Pb accumulation in sorghum crops grown in contaminated soils and their toxicological implications","resumen":"In this study, the content of Pb, the physico-chemical and biological parameters in soils, and the metal transfer to vegetative and reproductive Sorghum bicolor plants were evaluated along with their relationship with the toxicological risk of crop consumption. To carry this out, soil and sorghum samples at different growth stages were collected near to a former battery recycling plant. The results showed that the concentrations of Pb in soils at several sites were above the maximum permissible levels. Metal bioavailability was not directly related to the pH, OM% or EC, while no association between metals and the different genera of fungi was observed. Sorghum crops accumulated Pb mainly in the roots in all of the growth stages, and therefore presented low levels of Pb in aerial parts without toxicological risk due to direct consumption. Taken together, our results revealed that sorghum could be employed as a potential phytostabilizator of lead in soils associated with crop production. However, further studies are necessary to extend these findings.","palabras_clave":"Sorghum bicolor|Lead; Battery recycling plant|Phytostabilization|Food security"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4066,"idioma_id":4,"titulo":"Effects of co-cropping Bidens pilosa (L.) and Tagetes minuta (L.) on bioaccumulation of Pb  in Lactuca sativa (L.) growing in polluted agricultural soils.","resumen":"Polluted agricultural soils are a serious problem for food safety, with phytoremediation being the most favorable alternative from the environmental perspective. However, this methodology is generally time-consuming and requires the cessation of agriculture. Therefore, the purpose of this study was to evaluate two potential phytoextractor plants (the native species Bidens pilosa and Tagetes minuta) co-cropped with lettuce growing on agricultural lead-polluted soils. The concentrations of Pb, as well as of other metals, were investigated in the phytoextractors, crop species, and in soils, with the potential risk to the health of consumers being estimated. The soil parameters pH, EC, organic matter percentage and bioavailable lead showed a direct relationship with the accumulation of Pb in roots. In addition, the concentration of Pb in roots of native species was closely related to Fe (B. pilosa, r = 0.81; T. minuta r = 0.75), Cu (T. minuta, r = 0.93), Mn (B. pilosa, r = 0.89) and Zn (B. pilosa, r = 0.91; T. minuta, r = 0.91). Our results indicate that the interaction between rhizospheres increased the phytoextraction of lead, which was accompanied by an increase in the biomass of the phytoextractor species. However, the consumption of lettuce still revealed a toxicological risk from Pb in all treatments.","palabras_clave":"Co-cropping phytoremediation|Food safety|B. pilosa|T. minuta|L. sativa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4067,"idioma_id":4,"titulo":"Ursodeoxycholic and deoxycholic acids: Differential effects on intestinal Ca(2+) uptake, apoptosis and autophagy of rat intestine","resumen":"The aim of this work was to study the effect of sodium deoxycholate (NaDOC) and ursodeoxycholic acid (UDCA) on Ca(2+) uptake by enterocytes and the underlying mechanisms. Rats were divided into four groups: a) controls, b) treated with NaDOC, c) treated with UDCA d) treated with NaDOC and UDCA. Ca(2+) uptake was studied in enterocytes with different degrees of maturation. Apoptosis, autophagy and NO content and iNOS protein expression were evaluated. NaDOC decreased and UDCA increased Ca(2+) uptake only in mature enterocytes. The enhancement of protein expression of Fas, FasL, caspase-8 and caspase-3 activity by NaDOC indicates triggering of the apoptotic extrinsic pathway, which was blocked by UDCA. NO content and iNOS protein expression were enhanced by NaDOC, and avoided by UDCA. The increment of acidic vesicular organelles and LC3 II produced by NaDOC was also prevented by UDCA. In conclusion, the inhibitory effects of NaDOC on intestinal Ca(2+) absorption occur by decreasing the Ca(2+) uptake by mature enterocytes. NaDOC triggers apoptosis and autophagy, in part as a result of nitrosative stress. In contrast, UDCA increases the Ca(2+) uptake by mature enterocytes, and in combination with NaDOC acts as an antiapoptotic and antiautophagic agent normalizing the transcellular Ca(2+) pathway.","palabras_clave":"APOPTOSIS|AUTOPHAGY|Ca UPTAKE|UDCA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4068,"idioma_id":4,"titulo":"The length distribution of class I restricted T cell epitopes is determined by both peptide supply and MHC allele specific binding preference","resumen":"HLA class I-binding predictions are widely used to identify candidate peptide targets of human CD8(+) T cell responses. Many such approaches focus exclusively on a limited range of peptide lengths, typically 9 aa and sometimes 9-10 aa, despite multiple examples of dominant epitopes of other lengths. In this study, we examined whether epitope predictions can be improved by incorporating the natural length distribution of HLA class I ligands. We found that, although different HLA alleles have diverse length-binding preferences, the length profiles of ligands that are naturally presented by these alleles are much more homogeneous. We hypothesized that this is due to a defined length profile of peptides available for HLA binding in the endoplasmic reticulum. Based on this, we created a model of HLA allele-specific ligand length profiles and demonstrate how this model, in combination with HLA-binding predictions, greatly improves comprehensive identification of CD8(+) T cell epitopes.","palabras_clave":"MHC class I|Binding motifs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4069,"idioma_id":4,"titulo":"A combined prediction strategy increases identification of peptides bound with high affinity and stability to porcine MHC class I molecules SLA-1*04:01, SLA-2*04:01, and SLA-3*04:01","resumen":"Affinity and stability of peptides bound by major histocompatibility complex (MHC) class I molecules are important factors in presentation of peptides to cytotoxic T lymphocytes (CTLs). In silico prediction methods of peptide-MHC binding followed by experimental analysis of peptide-MHC interactions constitute an attractive protocol to select target peptides from the vast pool of viral proteome peptides. We have earlier reported the peptide binding motif of the porcine MHC-I molecules SLA-1*04:01 and SLA-2*04:01, identified by an ELISA affinity-based positional scanning combinatorial peptide library (PSCPL) approach. Here, we report the peptide binding motif of SLA-3*04:01 and combine two prediction methods and analysis of both peptide binding affinity and stability of peptide-MHC complexes to improve rational peptide selection. Using a peptide prediction strategy combining PSCPL binding matrices and in silico prediction algorithms (NetMHCpan), peptide ligands from a repository of 8900 peptides were predicted for binding to SLA-1*04:01, SLA-2*04:01, and SLA-3*04:01 and validated by affinity and stability assays. From the pool of predicted peptides for SLA-1*04:01, SLA-2*04:01, and SLA-3*04:01, a total of 71, 28, and 38 % were binders with affinities below 500 nM, respectively. Comparison of peptide-SLA binding affinity and complex stability showed that peptides of high affinity generally, but not always, produce complexes of high stability. In conclusion, we demonstrate how state-of-the-art prediction and in vitro immunology tools in combination can be used for accurate selection of peptides for MHC class I binding, hence providing an expansion of the field of peptide-MHC analysis also to include pigs as a livestock experimental model.","palabras_clave":"Swine|Stability|MHC|Peptide binding prediction;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4070,"idioma_id":4,"titulo":"Immunogenicity of HLA Class I and II Double Restricted Influenza A-derived Peptides","resumen":"The aim of the present study was to identify influenza A-derived peptides which bind to both HLA class I and -II molecules and by immunization lead to both HLA class I and class II restricted immune responses. Eight influenza A-derived 9-11mer peptides with simultaneous binding to both HLA-A*02:01 and HLA-DRB1*01:01 molecules were identified by bioinformatics and biochemical technology. Immunization of transgenic HLA-A*02:01\/HLA-DRB1*01:01 mice with four of these double binding peptides gave rise to both HLA class I and class II restricted responses by CD8 and CD4 T cells, respectively, whereas four of the double binding peptides did result in HLA-A*02:01 restricted responses only. According to their cytokine profile, the CD4 T cell responses were of the Th2 type. In influenza infected mice, we were unable to detect natural processing in vivo of the double restricted peptides and in line with this, peptide vaccination did not decrease virus titres in the lungs of intranasally influenza challenged mice. Our data show that HLA class I and class II double binding peptides can be identified by bioinformatics and biochemical technology. By immunization, double binding peptides can give rise to both HLA class I and class I restricted responses, a quality which might be of potential interest for peptide-based vaccine development.","palabras_clave":"Double Restricted Influenza A-Derived Peptides|Immunoinformatics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4071,"idioma_id":4,"titulo":"Toxoplasma gondii Peptide Ligands Open the Gate of the HLA Class I Binding Groove, Elife.","resumen":"HLA class I presentation of pathogen-derived peptide ligands is essential for CD8+ T-cell recognition of Toxoplasma gondii infected cells. Currently, little data exist pertaining to peptides that are presented after T. gondii infection. Herein we purify HLA-A*02:01 complexes from T. gondii infected cells and characterize the peptide ligands using LCMS. We identify 195 T. gondii encoded ligands originating from both secreted and cytoplasmic proteins. Surprisingly, T. gondii ligands are significantly longer than uninfected host ligands, and these longer pathogen-derived peptides maintain a canonical N-terminal binding core yet exhibit a C-terminal extension of 1-30 amino acids. Structural analysis demonstrates that binding of extended peptides opens the HLA class I F' pocket, allowing the C-terminal extension to protrude through one end of the binding groove. In summary, we demonstrate that unrealized structural flexibility makes MHC class I receptive to parasite-derived ligands that exhibit unique C-terminal peptide extensions.","palabras_clave":"human|immunology|infectious disease|microbiology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4072,"idioma_id":4,"titulo":"T cell recognition is shaped by epitope sequence conservation in the host proteome and microbiome","resumen":"Several mechanisms exist to avoid or suppress inflammatory T cell immune responses that could prove harmful to the host due to targeting self-antigens or commensal microbes. We hypothesized that these mechanisms could become evident when comparing the immunogenicity of a peptide from a pathogen or allergen with the conservation of its sequence in the human proteome or the healthy human microbiome. Indeed, performing such comparisons on large sets of validated T cell epitopes, we found that epitopes that are similar with self-antigens above a certain threshold showed lower immunogenicity, presumably as a result of negative selection of T cells capable of recognizing such peptides. Moreover, we also found a reduced level of immune recognition for epitopes conserved in the commensal microbiome, presumably as a result of peripheral tolerance. These findings indicate that the existence (and potentially the polarization) of T cell responses to a given epitope is influenced and to some extend predictable based on its similarity to self-antigens and commensal-antigens.","palabras_clave":"Bioinformatics|Epitopes|T cell recognition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4073,"idioma_id":4,"titulo":"Sequence diversity between class I MHC loci of African native and introduced Bos taurus cattle in Theileria parva endemic regions: in silico peptide binding prediction identifies distinct functional clusters.","resumen":"There is strong evidence that the immunity induced by live vaccination for control of the protozoan parasite Theileria parva is mediated by class I MHC-restricted CD8+ T cells directed against the schizont stage of the parasite that infects bovine lymphocytes. The functional competency of class I MHC genes is dependent on the presence of codons specifying certain critical amino acid residues that line the peptide binding groove. Compared with European Bos taurus in which class I MHC allelic polymorphisms have been examined extensively, published data on class I MHC transcripts in African taurines in T. parva endemic areas is very limited. We utilized the multiplexing capabilities of 454 pyrosequencing to make an initial assessment of class I MHC allelic diversity in a population of Ankole cattle. We also typed a population of exotic Holstein cattle from an African ranch for class I MHC and investigated the extent, if any, that their peptide-binding motifs overlapped with those of Ankole cattle. We report the identification of 18 novel allelic sequences in Ankole cattle and provide evidence of positive selection for sequence diversity, including in residues that predominantly interact with peptides. In silico functional analysis resulted in peptide binding specificities that were largely distinct between the two breeds. We also demonstrate that CD8+ T cells derived from Ankole cattle that are seropositive for T. parva do not recognize vaccine candidate antigens originally identified in Holstein and Boran (Bos indicus) cattle breeds.","palabras_clave":"Ankole cattle|Class I MHC;|CD8+ T cell epitopes|Theileria parva"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4074,"idioma_id":4,"titulo":"NetMHCpan-3.0; improved prediction of binding to MHC class I molecules integrating information from multiple receptor and peptide length data sets","resumen":"BACKGROUND:Binding of peptides to MHC class I molecules (MHC-I) is essential for antigen presentation to cytotoxic T-cells.RESULTS:Here, we demonstrate how a simple alignment step allowing insertions and deletions in a pan-specific MHC-I binding machine-learning model enables combining information across both multiple MHC molecules and peptide lengths. This pan-allele\/pan-length algorithm significantly outperforms state-of-the-art methods, and captures differences in the length profile of binders to different MHC molecules leading to increased accuracy for ligand identification. Using this model, we demonstrate that percentile ranks in contrast to affinity-based thresholds are optimal for ligand identification due to uniform sampling of the MHC space.CONCLUSIONS:We have developed a neural network-based machine-learning algorithm leveraging information across multiple receptor specificities and ligand length scales, and demonstrated how this approach significantly improves the accuracy for prediction of peptide binding and identification of MHC ligands. The method is available at www.cbs.dtu.dk\/services\/NetMHCpan-3.0 .","palabras_clave":"MHC|Epitope|Machine Learning"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4075,"idioma_id":4,"titulo":"Synthetic epoxy-pregnan steroids: Effects on anxiety behavior in rats","resumen":"Neurosteroids like 3a-OH-5a-pregnan-20-one(allopregnanolone)and 3a-OH-5b-pregnan-20-one(pregnanolone) modulate the g-aminobutyric acid-A(GABAA)receptor function and produce several effects that can beconsidered for therapeuticalpruposes like antidepressant and anxiolyticts, in asimilar way tobenzodiazepines. However, their rapid metabolism is a greatdisadvantage formedicinal treatments consideration. Synthetic steroid analogueswith morebioavailability and stability arise like a solution to overcometheselimitations. In previous studies, we evaluated the performance of asyntheticsteroid group (Epoxies, similar toallopregnanolone andpregnanolone) throughout different assays in rat cerebralcortex andhippocampus, including neuroprotection and GABAA receptormodulationobtaining promising results. Taking into account the anxiolyticeffect ofallopregnanolone and pregnanolone, in this work we evaluated theeffect of anintracranial administration (in dorsal hippocampus) of twosynthetic steroids, Epoxy1 and Epoxy 2, in an anxiety animal model toprovide knowledge aboutthe possible invivo effects of these steroids. Allopregnanolone andpregnanolone producedincreases in vertical and horizontal exploration behaviors, interpreted asanxiolytic-like effects. However, the two Epoxiescapableto modulate GABAA receptor binding have no effect on anxietyat thedose evaluated. These features make them suitable for othertherapeuticalpurposes where the anxiogenic behaviors are not involved.","palabras_clave":"NEUROSTEROIDS|SYNTHETICS ANALOGUES|ALLOPREGNENOLONE|OPEN FIELD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4076,"idioma_id":4,"titulo":"The importance of 2D aggregates on the antimicrobial resistance of Staphylococcus aureus sessile bacteria","resumen":"Biofilms formed on implanted devices are difficult to eradicate. Adhesion mechanism, high bacterial density, aggregation, induction of persisters and stressed bacteria are some of the factors considered when the antimicrobial resistance of these biofilms is analyzed. The aim of this work was to provide an alternative approach to the understanding of this issue by using a specially designed experimental set up that includes the use of microstructured (MS) surfaces (potential inhibitors of bacterial aggregation) in combination with antimicrobial agents (streptomycin and levofloxacin) against Staphylococcusaureus attached cells. Biofilms formed on smooth surfaces were used as plain controls (biofilmed-PC) characterized by the formation of dense 2D bacterial aggregates. Results showed bacterial persistence when streptomycin or levofloxacin were applied to PC-biofilms. The antimicrobial activity of both antibiotics was enhanced when bacteria were attached on MS, where single cells or small aggregates were observed. Thus, dense 2D aggregates of bacteria seem to be crucial as a required previous stage to develop the antimicrobial resistance.","palabras_clave":"biofilm|antibiotic|persister|surface topography"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4077,"idioma_id":5,"titulo":"LA ETNOHISTORIA EN LA UNIVERSIDAD DE BUENOS  AIRES: UN RECORRIDO POR 30 AÑOS DE INVESTIGACIONES","resumen":"En este trabajo ahondaremos en el desarrollo de la etnohistoria en el espacio particular de la sección homónima perteneciente alInstituto de Ciencias Antropológicas de la Facultad de Filosofía y Letras de la Universidad de Buenos Aires. Analizaremos la producciónde esta Sección como una variante más de todas las etnohistorias que, nosotros afirmamos, existen en la actualidad y quese han ido desarrollando según las dinámicas propias de los equipos de investigación de cada país en donde el campo disciplinarse desarrolló a partir de los años ochenta","palabras_clave":"Etnohistoria|UBA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4078,"idioma_id":5,"titulo":"Caracterización de la tenencia de animales de compañía en la Ciudad de Buenos Aires, Argentina","resumen":"El objetivo del estudio fue conocer características demográficas y de la tenencia de animales de compañía, así como las motivaciones, actitudes y conductas de la población que tiene o no animales, especialmente caninos, en la ciudad de Buenos Aires. Se diseñó un estudio social explicativo de prevalencia en dos áreas de la ciudad con diferentes estructuras socioeconómicas. En el grupo 1 (San Cristóbal) se encuestaron 357 viviendas y en el grupo 2 (Barrio Mitre) se encuestaron 169 viviendas. Se observó una alta proporción de animales por vivienda en ambas áreas. Los índices de tenencia de caninos por habitante entre las áreas difieren significativamente lo que puede atribuirse al nivel socioeconómico. Las diferencias en las proporciones de caninos hembra, de talla pequeña y con tendencia a razas puras con indicadores de mortalidad, natalidad y supervivencia variables indicarían que las poblaciones de animales de compañía tienden a ser reflejo de las condiciones sociales y vida de la población humana. Se observó que la esterilización puede emplearse para el control reproductivo o para resolver necesidades de la estrecha convivencia en el área urbana. Mayoritariamente, los caninos son incorporados a través del regalo, la compra o la recolección en la vía pública. La adopción no fue considerada como opción para obtener un canino. Los mapas cognitivos de tenencia de caninos para caracterizar cualitativa y cuantitativamente el vínculo entre animales y sus tenedores demostraron ser una novedosa forma de analizar la interrelación de variables.","palabras_clave":"TENENCIA|MOTIVACIONES|ANIMALES DE COMPAÑIA|CONDUCTA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4079,"idioma_id":4,"titulo":"Analysis and Simple Circuit Design of Double Differential EMG Active Electrode","resumen":"In this paper we present an analysis of the voltage amplifier needed for double differential (DD) sEMG measurements and a novel, very simple circuit for implementing DD active electrodes. The three-input amplifier that standalone DD active electrodes require is inherently different from a differential amplifier, and general knowledge about its design is scarce in the literature. First, the figures of merit of the amplifier are defined through a decomposition of its input signal into three orthogonal modes. This analysis reveals a mode containing EMG crosstalk components that the DD electrode should reject. Then, the effect of finite input impedance is analyzed. Because there are three terminals, minimum bounds for interference rejection ratios due to electrode and input impedance unbalances with two degrees of freedom are obtained. Finally, a novel circuit design is presented, including only a quadruple operational amplifier and a few passive components. This design is nearly as simple as the branched electrode and much simpler than the three instrumentation amplifier design, while providing robust EMG crosstalk rejection and better input impedance using unity gain buffers for each electrode input. The interference rejection limits of this input stage are analyzed. An easily replicable implementation of the proposed circuit is described, together with a parameter design guideline to adjust it to specific needs. The electrode is compared with the established alternatives, and sample sEMG signals are obtained, acquired on different body locations with dry contacts, successfully rejecting interference sources.","palabras_clave":"ACTIVE ELECTRODES|BIOMEDICAL SENSORS|DOUBLE DIFFERENTIAL ELECTRODES|DRY ELECTRODES|SURFACE ELECTROMYOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4080,"idioma_id":4,"titulo":"A simple and reproducible capacitive electrode","resumen":"Capacitive Electrodes (CE) allow the acquisition of biopotentials through a dielectric layer, without the use of electrolytes, just by placing them on skin or clothing, but demands front-ends with ultra-high input impedances. This must be achieved while providing a path for bias currents, calling for ultra-high value resistors and special components and construction techniques. A simple CE that uses bootstrap techniques to avoid ultra-high value components and special materials is proposed. When electrodes are placed on the skin; that is, with coupling capacitances CS of around 100 pF, they present a noise level of 3.3 µVRMS in a 0.5-100 Hz bandwidth, which is appropriate for electrocardiography (ECG) measurements. Construction details of the CE and the complete circuit, including a fast recovery feature, are presented.","palabras_clave":"ACTIVE ELECTRODES|INSULATING ELECTRODES|NON-CONTACT MEASUREMENTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4081,"idioma_id":4,"titulo":"Effect of the length of the suckling period and dietary energy intake in lactation on the duration of postpartum anestrus in Creole goats","resumen":"The objective of this study was to determine the effect of the length of the 21 suckling period and the dietary energy level on the duration of postpartum anestrus in 22 Creole goats. Twenty-eight multiparous pregnant goats (bodyweight BW 39.9 ± 6.4 kg; 23 mean ± SEM) each bearing a single fetus were used in a 2 suckling period (30 vs. 60 d) x 24 2 levels of energy intake (low (L) vs. high (H)) factorial design. Kids were separated 25 from their dams 3 d after birth and then suckled twice per day until weaned at 30 or 60 d 26 postpartum. Dams weaned at 30 d postpartum were hand milked for the next 30 d. Goats 27 were individually fed chopped alfalfa hay + balanced  concentrates (45: 55 DM basis) at 3 28 % of BW in the last month of pregnancy and at the same level to the L goats in the 29 postpartum period when the H goats were fed at 4 % of BW. Energy balance was 30 estimated by difference between energy intake and calculated energy requirements. 31 Duration of postpartum anestrus was estimated by determining the occurrence of 32 ovulation from changes in blood progesterone concentrations. There were no significant 33 lengths of suckling x energy intake level interactions. Goats on all treatments lost weight 34 until 45 d postpartum with more weight loss on the low than the high energy intake level 35 (L 4.06 ± 0.43 vs. H 1.99 ± 0.64 kg; P ","palabras_clave":"Goats|Reproduction|Anestrus|Dietary energy |Suckling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4082,"idioma_id":5,"titulo":"Adaptación de dos Medidas de Apoyo Social en una Muestra de Estudiantes Universitarios Argentinos","resumen":"El soporte social constituye un importante constructo en el ámbito educativo el cual influye en términos comportamentales, cognitivos y afectivos. Aunque en la literatura internacional existen numerosos instrumentos que permiten evaluar dicho constructo, en Argentina no se dispone de escalas que permitan evaluar el apoyo percibido por los estudiantes. Por tanto el objetivo de este trabajo consistió en adaptar las escalas de Apoyo Social para Niños y Adolescentes (Malecki, Demaray & Elliot, 2003) y Soporte Académico (Lent et al., 2005) en estudiantes universitarios. Para ello en este estudio participaron 551 estudiantes de diferentes carreras dictadas en la Universidad Nacional de Córdoba (Argentina). Para ambos instrumentos, los resultados indicaron valores satisfactorios de consistencia interna (confiabilidad compuesta, &#961; > .70) y además se logró replicar la estructura original de cada medida mediante análisis factorial confirmatorio. Se destacan las limitaciones y se sugieren nuevas líneas de estudio.","palabras_clave":"ESCALA DE APOYO SOCIAL PARA NIÑOS Y ADOLESCENTES|SOPORTE ACADÉMICO|SOPORTE SOCIAL|ESTUDIANTES UNIVERSITARIOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4083,"idioma_id":4,"titulo":"Plasma pharmacokinetics and muscle disposition of marbofloxacin in chickens","resumen":"sad","palabras_clave":"pharmacokinetics|marbofloxacin |chickens"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4084,"idioma_id":4,"titulo":"A first experience of flipped classroom in numerical analysis","resumen":"Flipped classroom is a pedagogical model that modifies the way that time is spent both in and outside the class, working with active learning, centered on the students. Do students accept flipping the classroom? What do they think about learning with recorded classes? These questions arise when the flipped model is to be implemented. A first experience of flipped classroom was carried out in a numerical analysis course at Facultad Regional San Nicolás, and students? opinions about this experience were gathered, so as to determine the acceptability of this methodology. The experience showed a high degree of acceptance by the students with respect to the methodology of the flipped class. Students were satisfied with the videos presented, and in general they preferred to work on their own in class, with the assistance of the faculty.","palabras_clave":"FLIPPED CLASSROOM|LEARNING STYLES|NUMERICAL ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4085,"idioma_id":4,"titulo":"Production of alpha-amylase from Aspergillus oryzae for several industrial applications in a single step","resumen":"A one-step method as a strategy of alpha-amylase concentration and purification was developed in this work. This methodology requires the use of a very low concentration of biodegradable polyelectrolyte (Eudragit® E-PO) and represents a low cost, fast, easy to scale up and non-polluting technology. Besides, this methodology allows recycling the polymer after precipitation.The formation of reversible soluble\/insoluble complexes between alpha-amylase and the polymer Eudragit® E-PO was studied, and their precipitation in selected conditions was applied with bioseparation purposes. Turbidimetric assays allowed to determine the pH range where the complexes are insoluble (4.50-7.00); pH 5.50 yielded the highest turbidity of the system. The presence of NaCl (0.05 M) in the medium totally dissociates the protein-polymer complexes.When the adequate concentration of polymer was added under these conditions to a liquid culture of Aspergillus oryzae, purification factors of alpha-amylase up to 7.43 and recoveries of 88% were obtained in a simple step without previous clarification. These results demonstrate that this methodology is suitablefor the concentration and production of alpha-amylase from this source and could be applied at the beginning of downstream processing.","palabras_clave":"ALPHA AMYLASE|PRODUCTION|YIELD|EUDRAGIT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4086,"idioma_id":5,"titulo":"Tuberculosis y migración hacia Córdoba durante las primeras décadas del siglo XX: discursos y concepciones en torno a la figura del migrante interno","resumen":"En este trabajo, proponemos ampliar el conocimiento que posee la historiografía especializada sobre las problemáticas de la migración y la tuberculosis en las primeras décadas del siglo XX, en el contexto de la migración interna que llegaba a la provincia y la ciudad de Córdoba.  Por un lado, abordamos el tipo de migración que llegaba a la ciudad en la marco de un sistema antituberculoso en construcción. Por el otro, analizamos los discursos y concepciones que surgieron desde los actores de poder local ante un escenario caracterizado por un tipo de migración de escasos recursos. En el trabajo identificamos la existencia de posturas que tendieron a convertir al inmigrante tuberculoso en el responsable tanto del contagio a los residentes ?sanos?, la ?invasión? de enfermos a la provincia y a la ciudad de Córdoba así como de llevar al sistema antituberculoso al límite de sus posibilidades de respuesta.","palabras_clave":"Migración interna|Tuberculosis|Córdoba|discursos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4087,"idioma_id":4,"titulo":"Salmonella enterica serovar Abortusequi as an emergent pathogen causing equine abortion in Argentine","resumen":"Salmonellosis is an infectious disease that affects humans,mammals, reptiles and poultry. It is produced by Salmonella spp.which is a gramnegative bacillus of 0.7-1.5 x 2-5 mm, facultativeanaerobes and nonsporulating. Salmonella enterica serovarAbortusequi (Salmonella Abortusequi) is a serovar adapted to thehost producing abortion in mares. If the animals do not abort,the neonates could born infected showing septicemia and polyarthritis.Salmonella Abortusequi was isolated from abortions in1948 for first time in Argentine. Between 1948 and 1994, abortionoutbreaks, mares with reproductive pathologies and goalswith septicemia and polyarthritis were described. Nowadays,the worldwide prevalence of paratific abortion is low but in ourcountry, the disease has been reported several times since 2011.The aims of this study were isolated and characterized SalmonellaAbortusequi from equine fetuses. Six fetuses with differentages were processed in The Laboratory of Infectious Diseases(LEEI) of Facultad de Ciencias Veterinarias, Universidad de BuenosAires","palabras_clave":"salmonella|equinos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4088,"idioma_id":4,"titulo":"Comparative study of emulsifying properties in acidic condition of soluble polysaccharides fractions obtained from soy hull and defatted soy flour","resumen":"The present study compares the emulsifying properties in acidic conditions of hull soluble polysaccharides (HSPS), soybean soluble polysaccharides (SSPS) and its mixtures. These fractions were obtained from byproducts of soybean processing industry (soy hull and residual fiber after isolation of soy cotyledon protein, respectively). Although SSPS is already characterized, HSPS is a novel fraction which has not been studied in deep and it is still unexplored as emulsifier. Dispersions of both fraction and a mixture 50:50 of them at pH 3.0 were used as aqueous phase (1.0?3.0 % w\/w) in coarse and fine oil-in-water emulsions (oil mass fraction= 0.3). Its stability was evaluated through the evolution of backscattering profiles (%BS), particle size distribution and mean particle diameters. The rheology of the emulsions was also analyzed. Both fractions provided stability to creaming when increasing the polysaccharide concentration and energy of homogenization. While coarse emulsions were unstable systems, fine emulsions were stable enough and allowed a deeper analysis of the destabilizing processes. A bridging flocculation phenomenon in the presence of HSPS and HSPS\/SSPS mixtures is suggested, which influences the creaming and rheological behavior. Also, coalescence index increases according HSPS and HSPS\/SSPS concentrations, but particle sizes reached were smaller than in SSPS emulsions. Fine emulsions with 3 % ofHSPS\/SSPS mixtures yielded the best results on the overall stability at 28 days. So, functional properties of the fractions may improve by the formulation of emulsions consisting in mixtures of them. These results are of interest to themanufacturing of acidic foods, taking advantage of obtaining byproducts from residual materials.","palabras_clave":"HULL SOLUBLE POLYSACCHARIDES|HULL PROTEINS|ACIDIC EMULSIONS|SOYBEAN SOLUBLE POLYSACCHARIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4089,"idioma_id":4,"titulo":"Pollen-specific aquaporins NIP4;1 and NIP4;2 are required for pollen development and pollination in Arabidopsis thaliana","resumen":"In flowers with dry stigmas, pollen development, pollination and pollen tube growth require spatial and temporal regulation of water and nutrient transport. To better understand the molecular mechanisms involved in reproductive processes, we characterized NIP4;1 and NIP4;2, two pollen-specific aquaporins (AQPs) of Arabidopsis thaliana. NIP4;1 and NIP4;2 are paralogs found exclusively in the angiosperm lineage. Although, they have 84% amino acid identity, they displayed different expression patterns. NIP4;1 has low expression levels in mature pollen while NIP4;2 peaks during pollen tube growth. Additionally, NIP4;1pro:GUS flowers showed GUS activity in mature pollen and pollen tubes, whereas NIP4;2pro:GUS flowers only in pollen tubes. Single T-DNA mutants and double amiRNA knockdowns had fewer seeds per silique and reduced pollen germination and pollen tube length. Functional assays in oocytes showed NIP4;1 and NIP4;2 function as water and non-ionic channels. We also found that NIP4;1 and NIP4;2 C-termini are phosphorylated by a pollen specific CPK modifying their water permeability. Survival assays in yeasts indicated NIP4;1 also transport ammonia, urea, boric acid and H2O2. Thus, we propose that aquaporins NIP4;1 and NIP4;2 are exclusive components of the reproductive apparatus of angiosperms with partial redundant roles in pollen development and pollination.","palabras_clave":"polen|germinacion|acuaporinas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4090,"idioma_id":4,"titulo":"Study of reproductive behaviour in low-chill apples in warmer zones of Argentina","resumen":"The domesticated apple (Malus × domestica Borkh.) shows abnormalities in its flowering biology when it is growing in mild-winter areas. Hence, the flowering phenology and the mating system (e.g., open-, cross- and self-pollination, apomixis and parthenocarpy) of Caricia, Eva, and Princesa apple cultivars were studied in three consecutive growing seasons. The flowering period ranged between August and October with wide variation between years, especially in relation to the overlapping of the full-bloom periods. Fruit set by selfing was significant, reaching a mean value of 20% with a minimum of 4.5% and a maximum of 43.7%, depending on the growing season and the cultivar. The self-incompatibility index showed significant changes between years in Eva and Princesa but not in Caricia. Fruit and seed set were significantly greater in cross- and open-pollination treatments than by self-pollination, apomixis or parthenocarpy. Those variables were affected by a growing season × treatment interaction. The results indicated that Caricia, Eva, and Princesa apples show partial to full self-incompatibility, depending strongly on the growing season. However, better fruit and seed set rates are obtained by cross-fertilization.","palabras_clave":"MALUS DOMESTICA|APOMIXIS|SELF-COMPATIBILITY|LACK OF CHILLING|FRUIT SET|PARTHENOCARPY|CROSS-COMPATIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4091,"idioma_id":4,"titulo":"Allelic differences in a vacuolar invertase affect Arabidopsis growth at early plant development","resumen":"Improving carbon fixation in order to enhance crop yield is a major goal in plant sciences. By quantitative trait locus (QTL) mapping, it has been demonstrated that a vacuolar invertase (vac-Inv) plays a key role in determining the radical length in Arabidopsis. In this model, variation in vac-Inv activity was detected in a near isogenic line (NIL) population derived from a cross between two divergent accessions: Landsberg erecta (Ler) and Cape Verde Island (CVI), with the CVI allele conferring both higher Inv activity and longer radicles. The aim of the current work is to understand the mechanism(s) underlying this QTL by analyzing structural and functional differences of vac-Inv from both accessions. Relative transcript abundance analyzed by quantitative real-time PCR (qRT-PCR) showed similar expression patterns in both accessions; however, DNA sequence analyses revealed several polymorphisms that lead to changes in the corresponding protein sequence. Moreover, activity assays revealed higher vac-Inv activity in genotypes carrying the CVI allele than in those carrying the Ler allele. Analyses of purified recombinant proteins showed a similar K m for both alleles and a slightly higher V max for that of Ler. Treatment of plant extracts with foaming to release possible interacting Inv inhibitory protein(s) led to a large increase in activity for the Ler allele, but no changes for genotypes carrying the CVI allele. qRT-PCR analyses of two vac-Inv inhibitors in seedlings from parental and NIL genotypes revealed different expression patterns. Taken together, these results demonstrate that the vac-Inv QTL affects root biomass accumulation and also carbon partitioning through a differential regulation of vac-Inv inhibitors at the mRNA level.","palabras_clave":"Arabidopsis|Quantitative Trait Loci|vacuolar invertase|biomass|Near Isogenic Line|inhibitor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4092,"idioma_id":4,"titulo":"Ground-based GNSS network and integrated water vapor mapping during the development of severe storms at the Cuyo region (Argentina)","resumen":"Mendoza is a province of Argentina located between 32° S and 34° S at the leeside of the Andes Foothills. Veryintense thunderstorms form between October and March (southern hemisphere summer), which producelarge hail and damage in crops and properties. Although some hypotheses and conceptual models were proposedin order to identify key possible mechanisms that contribute to trigger convection, they are still waiting for thevalidation process. As moisture is the main ingredient for storms formation, the identification of its geographicaldistribution could be used together with other synoptic and mesoscale forcing features to forecast intense convectiveevents. A novel technique in estimating moisture concentration and its geographical distribution hasbeen introduced in order to observe the influx and variability of humidity at this region, during a 45-day periodin midsummer. In doing so, we resort to the information provided by the ground-basedGlobal Navigation SatelliteSystem (GNSS) network. More than 300 active stations constitute the continuously operating GNSS networkover Southern and Central America (SIRGAS-CON, Sistema de Referencia Geocéntrico para las Américas deOperación Continua). This network allows to retrieve integrated water vapor (IWV) content, mapping this variableby the use of a digital model of terrain. In the period and region under study, a prevailing influx of humidityfrom N and NE and a high correlation between the accumulation\/depletion of humidity and the hail\/no hail precipitationdays is observed. We discuss in particular the development of five storms detected by the S-Band radarnetwork belonging to the Province of Mendoza. Although the results strongly suggest that IWV maps are capableto represent the humidity dynamics in the considered region, it is still important to highlight that the calculatedvalues for IWV are unrealistic at some locations as the consequence of deep atmospheric gradients. These biasesmay be explained by the fact that the GNSS observations are made over the whole horizon of each given site.","palabras_clave":"SIRGAS network|Ground-based GNSS meteorology|IWV mapping|Severe storms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4093,"idioma_id":5,"titulo":"Desempeño del sábalo (Prochilodus lineatus) en policultivo con pacú  (Piaractus mesopotamicus)","resumen":"En la piscicultura el 30% de los nutrientes aportados son retenidos como biomasa animal y el remanente se pierde en los sedimentos. Una alternativa es producir en policultivo, para aprovechar espacio y alimento. El objetivo del trabajo fue comparar y evaluar el crecimiento del sábalo Prochilodus lineatus en monocultivo, en contraste con policultivo de sábalo y pacú  Piaractus mesopotamicus durante 11 meses. Se utilizaron cuatro tratamientos, control (sábalo 100\/0), T1 (proporción sábalo-pacú 50\/50), T2 (proporción sábalo-pacú 33\/67), T3 (proporción sábalo-pacú 25\/75); ubicados en estanques de tierra fertilizados. Se suministró diariamente alimento balanceado considerando el 5% de la biomasa. Se registraron parámetros de calidad de agua, además de realizarse biometrías mensuales. Los parámetros físicos y químicos de calidad de agua permanecieron dentro de valores adecuados para ambas especies en cada estanque. Las variables zootécnicas analizadas fueron: coeficiente de crecimiento específico, conversión alimenticia, productividad, supervivencia y peso vivo. La variable peso en T3 fue superior, diferenciándose significativamente del control (P < 0,05). Los valores de las otras variables fueron más altos que en los tratamientos restantes, pero sin diferencias significativas (P < 0,05). Si bien no se encontraron diferencias en cuatro de las cinco variables estudiadas, se observó una tendencia a favor de T3, la que pudo no ser estadísticamente significativa debido al escaso número de réplicas disponibles y a la alta variabilidad. Sin embargo, siguiendo la tendencia obtenida y bajo las condiciones experimentales, el sábalo presentó un mejor crecimiento y supervivencia cuando se encontró acompañado del pacú en una relación 25\/75, constituyendo una alternativa satisfactoria para el cultivo de la especie.","palabras_clave":"cultivo semi-intensivo|factor de crecimiento específico|multiespecie|acuicultura"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4094,"idioma_id":5,"titulo":"Siete tesis en el horizonte. Nuevos Mitos y Viejas utopías para la enseñanza","resumen":"Hacia fines del siglo pasado, la Nueva Agenda de la Didáctica surgió como una fuerte interpelación hacia el abandono de la tradición tecnocrática y una apuesta potente hacia la paulatina resignificación de la educación formal como capaz de promover el pensamiento profundo. Las necesidades sociales eran entonces tan acuciantes como las urgencias disciplinares por superar la mirada ingenua, simplista y delgada de la enseñanza como método, que en última instancia diluía al sujeto en un protocolo de procedimientos tendientes a resultados. Mucho se había avanzado ya en la conceptualización del campo y, a medida que se difundieron trabajos e investigaciones, se fue consolidando en el discurso una metamorfosis plena de lo que se había entendido hasta entonces- muy delgadamente- como didáctica. El cambio, sin embargo, impregnó el niveldiscursivo sin llegar a encarnar en las prácticas. Las razones son múltiples y complejas, y solo se abordarán tangencial y conjeturalmente en este ensayo. En cambio, la presente publicación se propone delinear siete certezas adquiridas, vivas en el discurso, y problematizar respecto de algunas situaciones y condiciones que podrían gestar nuevos escenarios educativos consonantes con tales pronunciamientos.","palabras_clave":"DIDACTICA|TRANSFORMACIÓN EDUCATIVA|PRÁCTICA DOCENTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4095,"idioma_id":4,"titulo":"HYBRID NARRATIVE FOR DECOLONIAL PEDAGOGY","resumen":"Este artículo define y discute el rol de la narrativa híbrida en la construcción de alternativas políticas y metodológicas frente a la educación universitaria tradicional. Es por tanto motivado por el proceso de revisión y revalidación del legado eurocéntrico que ha estructurado a las instituciones del nivel superior, sus discursos y prácticas en naciones colonizadas como las nuestras. En un intento por superar tanto la completa negación de esa herencia como la supervivencia de los lazos que reproducen prerrogativas que favorecen sólo a algunos, hemos encontrado algunos modos sencillos de restaurar la perspectiva singular de lo local y la identidad particular de un entorno institucional que promueve la tradición y el respeto por las convenciones. Se analizarán lastensiones que surgen de estas tentativas, así como los resultados en términos de producción estudiantil.","palabras_clave":"NARRATIVA|PEDAGOGIA DECOLONIAL|FORMACIÓN SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4096,"idioma_id":5,"titulo":"Variations between 2 ECG as predictors of atrial fibrillation: Cohort study","resumen":"OBJETIVE:To evaluate the association between delta variations in the parameters of 2 sinusal ECG with atrial fibrillation (AF) onset.METHOD:Retrospective cohort of 9,975 adult patients and members of the prepaid system at Hospital Italiano de Buenos Aires from Argentina, who had at least 2 sinusal ECG between 2006 and 2011. Population was followed up for detection of AF. All measurements and electrocardiographic deltas (differences between the 2 ECG) were standardized. Hazard ratio (HR) was estimated for the development of AF for each delta of the different ECG parameters using a Cox regression model.RESULTS:During a median follow up of 3.5 years, 189 patients (1.89%) developed AF. Heart rate delta, ST interval delta and P wave amplitude were predictors of AF. Hazard ratio Adjusted for clinical characteristics and ECGbasal values was 0,86 (CI95%: 0.75-0.98, p=0.024) for heart rate delta, 1.12 (CI95%: 0.98-1.27, p=0.082) for ST interval delta and 1.21 (CI95%: 1.05-1.38, p=0.006) for P wave amplitude delta.CONCLUSION:Differences of heart rate and P wave amplitude between ECG's measurements may predict AF, independently of clinical features and ECGbasal values.","palabras_clave":"Fibrilación auricular|Electrocadiograma|Taquiarritmias|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4097,"idioma_id":4,"titulo":"Organic compounds present in airborne particles stimulate superoxide production and DNA fragmentation: role of NOX and Xanthine Oxidase in animal tissues","resumen":"Suspended particulate matter (SPM) trigger the production of reactive oxygen species. However, most of these studies focused on the effects of specific compounds and not real mixtures. In order to study the enzymatic superoxide (O2-) production resulting from exposition to a complex mixture, we derived organic extracts (OE) from SPM containing polycyclic aromatic hydrocarbons (PAHs) and exposed to mammal tissues. DNA damage was quantified employing the comet assay. In all tissues, studied enzymes were involved in O2- production when they were exposed, as the lucigenin?s chemiluminescence decays when enzymes were inhibited. Same trend was observed with the percentage of cells with comets, as the DNA damage was higher when they were exposed to same conditions. Our data allows us to hypothesize that these enzymes play an important role in oxidative stress by PAHs and that there is a mechanism involving them in O2-generation.&nbsp;","palabras_clave":"Suspended Particulate Matter|PAHs|Superoxide|Comet Assay"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4098,"idioma_id":4,"titulo":"Beat-to-beat electrocardiographic analysis of ventricular repolarization variability in patients after myocardial infarction","resumen":"Several studies have shown that the beat-to-beat variability ofventricular repolarization, which can be computed by Twave spectral variance(TSV) index, constitutes a marker of cardiac risk. Moreover, the fact thatproperties of actionpotential duration are altered during the healing (days, weeks) andhealed (months) infarct stages, have been reported. However, no data existregarding the influence of the time elapsed after myocardial infarction (MI) onmodulation of the beat-to-beat ventricular repolarization variability. In thepresent work we have evaluated TSV index during healing and healed stages of MIusing 12 standard ECG leads. The ECG of control or healthy subjects (n = 49)and the ECGs in patients after MI (n = 38), one within the first seven days (MI7)and the other after 60 days (MI60) of cardiac infarction, have been analyzed. Wehave considered the preferential ECG leads as those leads in which TSV indexhave presented a relative change greater than 10 in MI7 respect to control.Results indicate that TSV index have shown a significant increase (p &lt; 0.0005)in I, II, aVR, aVF, V3, V4, V5 and V6 leads in healing phase of MI (MI7) withrespect to control. Further, in the healed phase of MI (MI60), the TSV indextends to decrease their values towards the control. Also, we have computed amultilead TSV index based on the preferential ECG leads. In that sense,the multilead criteria have shown better perfomance quantifying beat-to-beatrepolarization variability than any single ECG lead considered. Thesensitivity, specificity and AUC of TSV index were: 92%, 90% and 0.96 for MI7;and 76%, 84% and 0.81 for MI60, respectively. Moreover, the beat-to-beat ventricularrepolarization variability has been quantified by the QT variability index(QTVI). Even though the results that we have obtained with TSV index have beencomparable to those obtained with the QTVI,this latter has not reflected the modulation effect associated to timeelapsed after MI. Also, the preferential ECG leads depending on MI site usingTSV index have been computed, being lead V4 for anterior and lead aVF forinferior MI, respectively. Finally, this study might help understand the role ofhealing and healed stages following MI on beat-to-beat variability modulationof ventricular repolarization.","palabras_clave":"T-wave spectral variance|Heterogeneity of ventricular repolarization|Myocardial infarction|Electrical remodeling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4100,"idioma_id":4,"titulo":"Descriptive analysis of high birth prevalence rate geographical  clusters of congenital anomalies in South America","resumen":"BACKGROUND:The birth prevalence rate (BPR) of congenital anomalies (CAs) is heterogeneous and exhibits geographical and sociocultural variations throughout the world. In South America (SA), high birth prevalence regions of congenital anomalies have been observed. The aim of this study was to identify, describe, and characterize geographical clusters of congenital anomalies in SA.METHODS:This observational descriptive study is based on clinical epidemiological data registered by the Latin-American Collaborative Study of Congenital Malformations network. Between 1995 and 2012, a total of 25,082 malformed newborns were ascertained from 2,557,424 births at 129 hospitals in SA. The spatial scan statistic was used to determine geographical regions with high BPR of CAs. The BPR was obtained with a Poisson regression model. Odds ratios were estimated for several risk factors inside the geographical clusters.RESULTS:We confirmed the existence of high BPR regions of CAs in SA. Indicators of low socioeconomic conditions, such as a low maternal education, extreme age childbearing, infectious diseases, and medicine use during pregnancy were detected as risk factors inside these regions. Native and African ancestries with high frequency of consanguineous marriages could explain partially these high BPR clusters.CONCLUSION:The recognition of clusters could be a starting point in the identification of susceptibility genes associated with the occurrence of CA in high BPR regions. Birth Defects Research (Part A) 106:257-266, 2016. © 2016 Wiley Periodicals, Inc.","palabras_clave":"ECLAMC|South America| birth prevalence rate|congenital anomalies"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4101,"idioma_id":4,"titulo":"Spanish semantic feature production norms for 400 concrete concepts","resumen":"Semantic feature production norms provide many quantitative measures of different feature and concept variables that are necessary to solve some debates surrounding the nature of the organization, both normal and pathological, of semantic memory. Despite the current existence of normsfor different languages, there are still no published norms in Spanish. This article presents a new set of norms collected from 810 participants for 400 living and nonliving concepts among Spanish speakers. These norms consist of empirical collections of features that participants used to describe the concepts. Four files were elaborated: a concept?feature file, a concept?concept matrix, a feature?feature matrix, and a significantly correlated features file. We expect that these normswill be useful for researchers in the fields of experimental psychology, neuropsychology, and psycholinguistics.","palabras_clave":"Feature norms|Spanish language|Semantic memory|Concepts"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4102,"idioma_id":4,"titulo":"A Genome-wide Association Study of Nonsyndromic Cleft Palate Identifies an Etiologic Missense Variant in GRHL3","resumen":"Cleft palate (CP) is a common birth defect occurring in 1 in 2,500 live births. Approximately half of infants with CP have a syndromic form, exhibiting other physical and cognitive disabilities. The other half have nonsyndromic CP, and to date, few genes associated with risk for nonsyndromic CP have been characterized. To identify such risk factors, we performed a genome-wide association study of this disorder. We discovered a genome-wide significant association with a missense variant in GRHL3 (p.Thr454Met [c.1361C>T]; rs41268753; p = 4.08 × 10(-9)) and replicated the result in an independent sample of case and control subjects. In both the discovery and replication samples, rs41268753 conferred increased risk for CP (OR = 8.3, 95% CI 4.1-16.8; OR = 2.16, 95% CI 1.43-3.27, respectively). In luciferase transactivation assays, p.Thr454Met had about one-third of the activity of wild-type GRHL3, and in zebrafish embryos, perturbed periderm development. We conclude that this mutation is an etiologic variant for nonsyndromic CP and is one of few functional variants identified to date for nonsyndromic orofacial clefting. This finding advances our understanding of the genetic basis of craniofacial development and might ultimately lead to improvements in recurrence risk prediction, treatment, and prognosis.","palabras_clave":"Cleft palate|cognitive disabilities|genome-wide"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4103,"idioma_id":4,"titulo":"A multi-ethnic genome-wide association study identifies novel loci for non-syndromic cleft lip with or without cleft palate on 2p24.2, 17q23 and 19q13.","resumen":"Orofacial clefts (OFCs), which include nonsyndromic cleft lip with or without cleft palate (CL\/P), are among the most common birth defects in humans, affecting approximately 1 in 700 newborns. CL\/P is phenotypically heterogeneous and has a complex etiology caused by genetic and environmental factors. Previous genome-wide association studies (GWAS) have identified at least 15 risk loci for CL\/P. As these loci do not account for all of the genetic variance of CL\/P, we hypothesized the existence of additional risk loci. We conducted a multiethnic GWAS in 6,480 participants (823 unrelated cases, 1700 unrelated controls, and 1319 case-parent trios) with European, Asian, African, and Central and South American ancestry. Our GWAS revealed novel associations on 2p24 nearFAM49A, a gene of unknown function (p=4.22x10-8), and 19q13 nearRHPN2, a gene involved in organizing the actin cytoskeleton (p=4.17x10-8). Other regions reaching genome-wide significance were 1p36 (PAX7), 1p22 (ARHGAP29), 1q32 (IRF6), 8q24, and 17p13 (NTN1), all reported in previous GWASs. Stratification by ancestry group revealed a novel association with a region on 17q23 (p=2.92x10-8) among individuals with European ancestry. This region included several promising candidates includingTANC2, an oncogene required for development, andDCAF7, a scaffolding protein required for craniofacial development. In the Central and South American ancestry group, significant associations with loci previously identified in Asian or European ancestry groups reflected their admixed ancestry. In summary, we have identified novel CL\/P risk loci and suggest new genes involved in craniofacial development, confirming the highly heterogeneous etiology of OFCs.","palabras_clave":"Orofacial clefts|multiethnic|genome-wide association"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4104,"idioma_id":5,"titulo":"Evidencias químicas de preparación de alimentos en  la alfarería de la Depresión del río Salado.","resumen":"La preparación, el consumo y el almacenamiento de alimentos en cacharros de cerámica dejan residuos que penetran en la matriz porosa de los recipientes. En este trabajo se buscó reconocer algunos de esos residuos aplicando la técnica de análisis químico de cromatografía de gases. Se presentan los resultados de los estudios de 15 muestras de fragmentos cerámicos arqueológicos pertenecientes a grupos cazadores-recolectores-pescadores que habitaron en la microrregión del río Salado bonaerense durante el Holoceno tardío. La aplicación del análisis estadístico multivariado nos permitió agrupar a las muestras estudiadas, captar la mayor variabilidad del conjunto y  lograr una mejor precisión en los resultados. A través de estos estudios se identificaron diversos tipos de alimentos que permitieron generar información sobre las prácticas de cocción y consumo de diferentes recursos. En sus prácticas diarias de cocción o servido de alimentos, los grupos de la Depresión del río Salado cocinaron carnes de mamíferos medianos y carne de pescado en diferentes enseres de alfarería, mientras que otros cacharros estuvieron asociados con la manipulación de productos vegetales. Palabras clave: cerámica; alimentos; análisis químicos; río Salado bonaerense.","palabras_clave":"CERÁMICA|ANALISIS GRASOS|ALIMENTOS|RIO SALADO BONAERENSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4105,"idioma_id":5,"titulo":"Estereotipos al descubierto. Una propuesta didáctica para problematizar lo masculino y lo femenino en el aula","resumen":"En este escrito se presentan una serie de actividades correspondientes a una unidad didáctica de educación sexual elaborada por estudiantes del profesorado de biología de la Facultad de Ciencias Exactas y Naturales (UBA) en conjunto con docentes tutores. El propósito central de las propuestas didácticas presentadas es aportar a que en la escuela se habiliten espacios de reflexión sobre la construcción de la sexualidad entendiendo que hay infinitas formas de ser, vivir y sentir y que estas no tienen porqué responder a un mandato impuesto. Nos centramos en aportar a desnaturalizar los estereotipos identitarios impuestos, reproducidos por la institución escolar y muchas veces legitimados desde las ciencias biológicas. El trabajo presentado cobra especial relevancia ya que proviene de una construcción colectiva en el marco de la formación inicial, donde generalmente lxs docentes de biología poseen escasas instancias para comprender la sexualidad en un sentido amplio, más allá de la genitalidad, la reproducción y de una mirada patologista.","palabras_clave":"Estereotipos de género | educación sexual|docentes de biología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4106,"idioma_id":4,"titulo":"The overt pronoun constraint across three dialects of Spanish","resumen":"The overt pronoun constraint (OPC) states that, in null subject languages, overtpronoun subjects of embedded clauses cannot be bound by wh- or quantifier antecedents.Through the administration of two written questionnaires, we examined the OPC in 246monolingual native speakers of three dialects of Spanish, spoken in Barranquilla (Colombia),Santiago (Chile), and Buenos Aires (Argentina). We tested separately the predictions thatovert pronouns cannot be bound by wh- antecedents (Experiment 1) and that they cannot bebound by quantifier antecedents (Experiment 2).We found that the OPC was not operative inany of these dialects. In Experiment 1, the percentage of bound answers was approximatelythe same as the percentage of anaphoric answers. In Experiment 2, the percentage of boundanswers was significantly higher than the percentage of anaphoric answers. Implicationsboth for theories of pronoun resolution in null subject languages and for theories of first andsecond language acquisition are discussed.","palabras_clave":"OPC|SPANISH|ANAPHORA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4107,"idioma_id":4,"titulo":"Morphometric Differentiation Among Anastrepha fraterculus (Diptera: Tephritidae) Exploiting Sympatric Alternate Hosts","resumen":"Anastrepha fraterculus (Wiedemann) is currently considered a complex of cryptic species infesting fruits fromMexico to Argentina and represents an interesting biological model for evolutionary studies. Moreover, detecting and quantifying behavioral, morphological, and genetic differentiation among populations is also relevant to the application of environment-friendly control programs. Here, phenotypic differentiation among individuals coexisting in the wild in a Northern region of Argentina was unveiled and associated with host choice. Six morphometric traits were measured in sympatric flies exploiting three different host species. Phenotypic variation was shown to be host-dependent regardless of geographical or temporal overlap. Flies collected from synchronous alternate hosts (peach and walnut) differed from each other despite the lack of geographical isolation. By contrast, flies emerging from guavas that ripen about two months later than peach and walnut showed no significant differentiation in comparison to flies collected from walnuts, but they differ significantly from flies originating from peaches. This result is consistent with the hypothesis that the same population of flies shifts from walnuts to guavas throughout the year, whereas the population of flies that uses peaches as a host is probably exploiting other alternate hosts when peach availability decreases. Further research is needed to study the underlying mechanism. Results are consistent with previous molecular markers (inter-simple sequence repeat- ISSR) research on flies stemming from the same hosts and the same area, suggesting that differentiationamong flies emerging from alternative hosts occurs at both genetic and phenotypic levels. The contribution of host preference in long-term genetic differentiation is discussed.","palabras_clave":"SOUTH AMERICAN FRUIT FLY|MORPHOMETRY|BAYESIAN ANALYSIS|SPECIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4108,"idioma_id":4,"titulo":"Raman in situ characterization of the species present in Co\/CeO2 and Co\/ZrO2 catalysts during the COPrOx reaction","resumen":"The in situ Raman spectroscopy constitutes a powerful procedure to characterize catalytic surfaces under reaction conditions. In this work, we studied the species present in Co\/CeO2 and Co\/ZrO2 catalysts during the COPrOx reaction carried out between room temperature and 500 C. For both catalysts, TPR and Raman results suggest that a redox mechanism proceeds in which the reduction step with either hydrogen or CO is the rate-limiting step. The CeO2 support is better than the ZrO2 one because the former accelerates the surface exchange between reduced and oxidized species due to the high mobility of surface lattice oxygen and the presence of oxygen vacancies. The Ce4þ þ Co2þ4Ce3þ þ Co3þ process acts as a buffer effect by which Co3þ, which is the active species in this reaction, is always present even in a reducing atmosphere, as shown by in situ Raman characterization.","palabras_clave":"COPROX|RAMAN IN SITU"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4109,"idioma_id":4,"titulo":"Genome-wide Analysis of the Snakin\/GASA Gene Family in Solanum tuberosum cv. Kennebec","resumen":"Snakin\/GASA proteins have been involved in different aspects of plant growth and development although their exact role is still intriguing. All of them maintain 12 cysteines of the C-terminus in highly conserved positions that are responsible for their structure and are essential for their biochemical activity as antioxidants. Two members were isolated from Solanum tuberosum to date (Snakin-1 and Snakin-2) and were shown to have antimicrobial activity. We have recently demonstrated that Snakin-1 has additional roles in plant growth and development. We carried out a genome-wide search for new Snakin\/GASA family members in potato. 16 Snakin\/GASA genes were isolated, sequenced and characterized. Interestingly, we found in Solanum tuberosum subsp. tuberosum cv. Kennebec that Snakin-1, Snakin-2 and Snakin-3 expression is affected by bacterial and\/or fungal inoculation. These results strengthen the participation of Snakin-1 and Snakin-2 genes in biotic stress tolerance and suggest that Snakin-3 is also involved in plant defense. The data presented here could be a good starting point for more focused and deep investigations regarding the biological functions of potato Snakin\/GASA genes during plant development and in response to environmental stress.","palabras_clave":"Snakin\/GASA|cysteine-rich domain|potato|plant development and stress responses"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4110,"idioma_id":4,"titulo":"Evolutionary Conserved Positions Define Protein Conformational Diversity","resumen":"Conformational diversity of the native state plays a central role in modulating protein function. The selection paradigm sustains that different ligands shift the conformational equilibrium through their binding to highest-affinity conformers. Intramolecular vibrational dynamics associated to each conformation should guarantee conformational transitions, which due to its importance, could possibly be associated with evolutionary conserved traits. Normal mode analysis, based on a coarse-grained model of the protein, can provide the required information to explore these features. Herein, we present a novel procedure toidentify key positions sustaining the conformational diversity associated to ligand binding. The method is applied to an adequate refined dataset of 188 paired protein structures in their bound and unbound forms. Firstly, normal modes most involved in the conformational change are selected according to their corresponding overlap with structural distortions introduced by ligand binding. The subspace defined by these modes is used to analyze the effect of simulated point mutations on preserving the conformational diversity of the protein.We find a negative correlation between the effects of mutations on these normal mode subspaces associated to ligand-binding and position-specific evolutionary conservations obtained from multiple sequence-structure alignments. Positions whose mutations are found to alter the most these subspaces are defined as key positions, that is, dynamically important residues that mediate the ligand-binding conformational change. These positions are shown to be evolutionary conserved, mostly buried aliphatic residues localized in regular structural regions of the protein like &#946;-sheets and &#945;-helix.","palabras_clave":"evolution|proteins|conformational diversity|normal modes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4111,"idioma_id":4,"titulo":"Fuzzy Human Reliability Analysis: Applications and Contributions Review","resumen":"The applications and contributions of fuzzy set theory to human reliability analysis (HRA) are reassessed. The main contribution of fuzzy mathematics relies on its ability to represent vague information. Many HRA authors have made contributions developing new models, introducing fuzzy quantification methodologies. Conversely, others have drawn on fuzzy techniques or methodologies for quantifying already existing models. Fuzzy contributions improve HRA in five main aspects: (1) uncertainty treatment, (2) expert judgment data treatment, (3) fuzzy fault trees, (4) performance shaping factors, and (5) human behaviour model. Finally, recent fuzzy applications and new trends in fuzzy HRA are herein discussed.","palabras_clave":"FUZZY HUMAN RELIABILITY|FUZZY CONTRIBUTIONS|FUZZY APPLICATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4112,"idioma_id":5,"titulo":"Latinoamerica y sus políticas públicas en pro de los derechos humanos","resumen":"Alors que la lutte pour les droits de l´homme est toujours une tâche inachevée, la dialectique et souvent retardé, il est nécessaire de réfléchir sur les réalités se sont produites en Amérique latine dans la dernière décennie, liée au progrès dans les droits de l´homme, en tenant compte des différences et des possibilités chaque pays, les alignements régionaux, des cadres politiques et idéologiques et des configurations qui signifie qu´ils ont été matérialisent dans la région, des actions conjointes (et parfois pas) comme CELAC, UNASUR, MERCOSUR, ALBA la Banque de l´ALBA, le SUCRE, et d´autres initiatives. Ces décisions ont été encadrées, plus souvent à la recherche de l´intégration dans la région pour la consolidation des processus démocratiques qui cherchent à transformer la consolidation de la souveraineté, incarnée par une série de mesures qui visent à ratifier la identité régionale et la construction d´une région plus juste.","palabras_clave":"Amérique latine |Droits de l'Homme |Meanings"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4113,"idioma_id":4,"titulo":"Novel sulfamides and sulfamates derived from amino esters: Synthetic studies and anticonvulsant activity","resumen":"We report herein the design and optimization of a novel series of sulfamides and sulfamates derived from amino esters with anticonvulsant properties. The structures were designed based on the pharmacophoric pattern previously proposed, with the aim of improving the anticonvulsant action. Thecompounds were obtained by a new synthetic procedure with microwave assisted heating and the use of adsorbents in the isolation process. All the derivatives showed protection against the maximal electroshock seizure test (MES test) in mice at the lowest dose tested (30 mg\/kg) but they did not show significantprotection against the chemical induced convulsion by pentylenetetrazole. These results verify the ability of the computational model for designing new anticonvulsants structures with anti-MES activity. Additionally, we evaluated the capacity of the synthesized structures to bind to the benzodiazepinebinding site (BDZ-bs) of the &#947;-aminobutiric acid receptor (GABAA receptor). Some of them showed medium to low affinity for the BDZ-bs.","palabras_clave":"EPILEPSY|SULFAMIDES|SULFAMATES|MES TEST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4114,"idioma_id":4,"titulo":"Nucleation and growth of graphite in eutectic spheroidal cast iron: Modeling and testing","resumen":"A new model of graphite growth during the continuous cooling of eutectic spheroidal cast iron is presented in this paper. The model considers the nucleation and growth of graphite from pouring to room temperature. The microstructural model of solidification accounts for the eutectic as divorced and graphite growth rate as a function of carbon gradient at the liquid in contact with the graphite. In the solid state, the microstructural model takes into account three stages for graphite growth, namely: (i) from the end of solidification to the upper bound of stable eutectoid intercritical; (ii) during the stable eutectoid intercritical, and (iii) from the lower bound of stable eutectoid intercritical to room temperature. The micro and macrostructural models are coupled using a sequential multiscale approach. Numerical results for graphite fraction and size distribution are compared with experimental results obtained from a cylindrical cup, in which the graphite volumetric fraction and size distribution were obtained using the Schwarz-Saltykov approach. The agreements between the experimental and numerical results for the fraction of graphite and the size distribution of spheroids reveals the importance of numerical models in the prediction of the main aspects of graphite in spheroidal cast iron.","palabras_clave":"spheroidal cast iron|graphite|thermo-metallurgical|finite element method"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4115,"idioma_id":4,"titulo":"AtPDCD5 Plays a Role in Programmed Cell Death after UV-B Exposure in Arabidopsis","resumen":"DNA damage responses have evolved to sense and react to DNA damage; the induction of DNA repair mechanisms can lead to genomic restoration or, if the damaged DNA cannot be adequately repaired, to the execution of a cell death program. In this work, we investigated the role of an Arabidopsis protein, AtPDCD5, which is highly similar to the human PDCD5 protein; it is induced by UV-B radiation and participates in programmed cell death (PCD) in the UV-B DNA damage response. Transgenic plants expressing AtPDCD5 fused to GFP indicate that AtPDCD5 is localized both in the nucleus and the cytosol. By use of pdcd5 mutants, we here demonstrate that these plants have an altered antioxidant metabolism and accumulate higher levels of DNA damage after UV-B exposure, similar to levels in ham1ham2 RNAi transgenic lines with decreased expression of acetyltransferases from the MYST family. By coimmunoprecipitation and pull down assays, we provide evidence that AtPDCD5 interacts with HAM proteins, suggesting that both proteins participate in the same pathway of DNA damage responses. Plants overexpressing AtPDCD5 show less DNA damage but more cell death in root tips upon UV-B exposure. Finally, we here show that AtPDCD5 also participates in age-induced programmed cell death. Together, the data presented here demonstrate that AtPDCD5 plays an important role during DNA damage responses induced by UV-B radiation in Arabidopsis and also participates in PCD programs.","palabras_clave":"DNA repair|Programmed cell death|arabidopsis|UV-B"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4116,"idioma_id":4,"titulo":"P1 epigenetic regulation in leaves of high altitude maize landraces: effect of UV-B radiation","resumen":"P1isaR2R3-MYBtranscriptionfactorthatregulatestheaccumulationofaspecificgroupofflavonoidsinmaizefloraltissues,suchasflavonesandphlobaphenes.P1isalsohighlyexpressedinleavesofmaizelandracesadaptedtohighaltitudesandhigherlevelsofUV-Bradiation.Inthiswork,weanalyzedtheepigeneticregulationoftheP1genebyUV-Binleavesofdifferentmaizelandraces.OurresultsdemonstratethatDNAmethylationintheP1proximalpromoter,intron1andintron2isdecreasedbyUV-Binalllinesanalyzed;however,thebasalDNAmethylationlevelsarelowerinthelandracesthaninB73,alowaltitudeinbredline.DNAdemethylationbyUV-BisaccompaniedbyadecreaseinH3methylationatLys9and27,andbyanincreaseinH3acetylation.smRNAscomplementarytospecificregionsoftheproximalpromoterandofintron23&#8242;endarealsodecreasedbyUV-B;interestingly,P1smRNAlevelsarelowerinthelandracesthaninB73bothundercontrolconditionsandafterUV-Bexposure,suggestingthatsmRNAsregulateP1expressionbyUV-Binmaizeleaves.Finally,weinvestigatedifdifferentP1targetsinflowertissuesarealsoregulatedbythistranscriptionfactorinresponsetoUV-B.SometargetsanalyzedshowaninductioninmaizelandracesinresponsetoUV-B,withhigherbasalexpressionlevelsinthelandracesthaninB73;however,notallthetranscriptsanalyzedwerefoundtoberegulatedbyUV-Binleaves.","palabras_clave":"P1|flavonoides|maiz|UV-B"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4117,"idioma_id":4,"titulo":"The  Proteolytic Activity of Philibertia gilliesii Latex. Purification of Philibertain g II","resumen":"The latex from the patagonic plant Philibertia gilliesii Hook. et Arn.(Apocynaceae) is a milky-white suspension containing a proteolytic system constituted by several cysteine endopeptidases. A proteolytic preparation (philibertain g) from the latex of P. gilliesii fruits was obtained and characterized to evaluate its potential use in bioprocesses. Philibertain g contained 1.2 g\/L protein and a specific (caseinolytic) activityof 7.0 Ucas\/mg protein. It reached 80 % of its maximum caseinolytic activity in the pH 7?10 range, retained 80 % of the original activity after 2 h of incubation at temperatures ranging from 25 to 45 °C and could be fully inactivated after 5 min at 75 °C. Philibertain gretained 60 % of the initial activity even at 1 M NaCl and was able to hydrolyze proteins from stickwater, one of the main waste effluents generated during fishmeal production.Furthermore, as a contribution to the knowledge of the proteolytic system of P. gilliesii, we are reporting the purification of a new peptidase, named philibertain g II (pI 9.4, molecular mass 23,977 Da, N-terminus LPESVDWREKGVVFPXRNQ) isolated from philibertain g through a purification scheme including acetone fractionation, cation exchange, molecularexclusion chromatography, and ultrafiltration.","palabras_clave":"Proteases|Mass spectrometry|Enzymes|Philibertia|gilliesii"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4118,"idioma_id":4,"titulo":"CeO2 and Co3O4 CeO2 nanoparticles: effect of the synthesis method on the structure and catalytic properties in COPrOx and methanation reactions","resumen":"CeO2 and Co3O4?CeO2 nanoparticles were synthesized, thoroughly characterized, and evaluated in the COPrOx reaction. The CeO2 nanoparticles were synthesizedby the diffusion-controlled precipitation method with ethylene glycol. A notably higher yield was obtained when H2O2 was used in the synthesis procedure. For comparison, two commercial samples of CeO2 nanoparticles (Nyacol)?one calcined and the other sintered?were also studied. Catalytic results of bare CeO2 calcined at 500 C showed a strong influence of the method of synthesis. Despite having similar BET area values, the CeO2 synthesized without H2O2 was the most active sample. Co3O4?CeO2 catalysts with three different Co\/(Co ? Ce) atomic ratios, 0.1, 0.3, and 0.5, were prepared by the wet impregnation of the CeO2 nanoparticles. TEM and STEM observations showed that impregnation produced mixed oxides composed of small CeO2 nanoparticles located both over the surface and inside the Co3O4 crystals. The mixed oxide catalysts prepared with a cobalt atomic ratio of 0.5 showed methane formation, which started at 200 C due tothe reaction between CO2 and H2. However, above 250 C, the reaction between CO and H2 became important, thus contributing to CO elimination with a small H2 loss. As a result, CO could be totally eliminated in a wide temperaturerange, from 200 to 400 C. The methanation reaction was favored by the reduction of the cobalt oxide, as suggested by the TPR experiments. This result is probably originated in Ce?Co interactions, related to the method of synthesis and the surface area of the mixed oxides obtained.","palabras_clave":"Ceria|Cobalt|Nanoparticles|COPrOx"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4119,"idioma_id":4,"titulo":"Complexing capacity of electroplating rinsing baths?a twist to the resolution of two ligand families of similar strength","resumen":"The presence of ligands affects metal behavior when removing metals from wastewaters. So far, most of the attention has been paid to strong ligands; however, experimental observations indicate that also weaker complexing agents may play a key role in the availability of metals in waters and in the success of the treatment tobe applied. In particular, we have analyzed wastewaters of an electroplating facility during an 8-h workday. Total metal content (copper, zinc, cadmium, and lead) was determined; ligands were characterized by concentration (Lt) and strength (conditional stability constant, K'f). This work focuses on ligands of moderate strength which, as far as we know, have been overlooked in the literature.","palabras_clave":"COMPLEXING CAPACITY|MODERATE LIGANDS|ELECTROPLATING BATH|ZINC|CADMIUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4121,"idioma_id":4,"titulo":"Influence of recycled aggregates on properties of self-consolidating concretes","resumen":"Recycled aggregates are made from crushed waste concretes and can be used as a replacement of natural aggregates in concrete production. Despite having lower density and higher absorption than natural aggregates, they can be used to manufacture conventional concretes with good performance if they are added in the proper amounts. To make self-consolidating concretes, the same aggregates used to manufacture conventional concretes can be used, but in order to increase segregation resistance and keep mix cohesion, a large amount of fine aggregates and a suitable admixture are required. The main goal of this work is to study the influence of recycled aggregates on self-consolidating concrete. Concretes were mixed with 50% of the coarse aggregate replaced by recycled aggregates (Patagonia gravel) and with 20% of the fine aggregate (natural sand) replaced by crushed concrete powders. Fresh concrete properties were tested, and physical and mechanical properties were determined in the hardened state. The petrographic composition of the concrete was examined to assess the interfacial transition zone and the contribution of the powders to the mortar microtexture. The results vary depending on the type of admixture and aggregate. However, it is shown that the inclusion of these crushed aggregates to make good self-consolidating concrete is feasible.","palabras_clave":"self-consolidating concretes|recycled aggregates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4122,"idioma_id":4,"titulo":"Logging by selective extraction of best trees: does it change patterns of genetic diversity? The case of Nothofagus pumilio","resumen":"Extensive knowledge of the ecological and genetic consequences ofimplementing management practices (i.e. logging) in natural ecosystems is of fundamentalimportance to conservation action. Accordingly, characterization of forestgenetic resources in managed vs non-managedstands may inform management decisions to ensure the long-term persistence ofgenetic diversity. The main objective of this study was to evaluate the impactof management practices on the genetic diversity and spatial genetic structureof contrasting forests, through an age-class sampling design and the use of microsatellitemarkers. We evaluated the impact of logging in three populations of Nothofagus pumilio, a dominant treespecies in Patagonian temperate forests, by comparing managed and non-managedstands in each population. Selective extraction of best-featured individuals,i.e. those with forestry aptitude, such as higher trees with straight trunks and good sanitary conditions, was performed between 1990 and2004. One of the studied sites was located in a state-protected area while the otherswere on private land affected by grazing. At each managed stand over-mature trees (MF-O), adultremnant trees (MF-A) and seedlings representing forest regeneration (MF-R)after silvicultural management were sampled. In non-managed stands age classeswere restricted to adults (CF-A) and seedlings (CF-R). A minimum of 30individuals per age class were collected, totalling 454 samples which were genotypedat six microsatellite loci.Non-significant differences in genetic diversity were found betweenmanaged and natural woods in all populations. A trend towards decreasingfrequencies or even allele loss among remnant adults of logged stands can howeverbe interpreted as a sign of impact, probably a consequence of genetic drift. Eachsite showed particular, different outcomes with respect to genetic structure.While in Pop 1 (Huemules, 42°S) significant genetic differentiation was found betweenmanagement treatments, admixture of genetic clusters (Bayesian clustering andDAPC analysis) occurred in Pop 2 (Guacho Lake, 43°S) and no genetic structure wasfound in Pop 3 (Engaño Lake, 43°S). Post-harvest genetic contact betweencontrasting stands is likely. A Landscape Interpolation Analysis showed clustersof individuals (shared genotypes) spatially restricted for managed stands (significantin Pop 3), whereas a random spatial distribution characterized control forests.Therefore, it is possible that management affected and disrupted the geneticstructure. The different genetic patterns revealed for eachpopulation call for site-by-site interpretation. Differential intensity and frequencyof management practices, presence\/absence of livestock in the forest, andevolutionary history may all have had combined effect on current geneticdiversity.","palabras_clave":"NOTHOFAGUS PUMILIO|LOGGING|AGE CLASSES|MICROSATELLITES|GENETIC DIVERSITY|SPATIAL GENETIC STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4123,"idioma_id":4,"titulo":"Eprinomectin accumulation in Rhipicephalus (Boophilus) microplus: Pharmacokinetic and efficacy assessment","resumen":"Eprinomectin (EPM) is a macrocyclic lactoneused against endo-ectoparasites without withdrawal time in milk and meat afterits pour-on administration at 0.5 mg\/kg. Previous experiments evaluated theefficacy of EPM against Rhipicephalus (Boophilus) microplus in cattle. Thisstudy assessed EPM efficacy against R. (B.) microplus after topicaladministration at two dose rates and investigated the relationship between EPMsystemic exposure in the host and drug concentrations accumulated in ticksrecovered from treated animals. A standardized pharmaco-parasitological studywas performed in two phases. In phase 1 eighteen Braford cattle naturallyinfected with R. (B.) microplus were divided into three experimental groupswith a similar level of infestation (Kruskal?Wallis test, P &gt; 0.05): controlgroup and treated groups with EPM pour-on (1 and 1.5 mg\/kg). Samples ofheparinized blood and ticks at different life stages were taken between 0 and21 days (d) post-administration to measure EPM concentrations by HPLC. Theefficacy trial (phase 2) included eighteen Braford calves naturally infectedwith R. (B.) microplus divided into control group and 1 mg\/kg and 1.5 mg\/kg EPMtreated groups. Female ticks (4.5?8 mm) on cattle were counted between 1 and 23days post-treatment to evaluate the efficacy of EPM. The reproductiveefficiency index (REI) and the fertility efficiency index (FEI) were evaluated.Plasma concentrations of EPM showed a linear relationship with the level ofdose rate administered. Peak plasma concentrations were within a range between13.8 and 90 ng\/ml, which guarantee milk drug concentrations below the maximumresidues level. High EPM concentrations were detected in ticks. EPMconcentrations in R. (B.) microplus were correlated to plasma concentrationsbetween 1.25 days and 21 days post-administration (r 0.84; P &lt; 0.05). EPMefficacy calculated using the Henderson?Tilton formula was 98.9% and 99.1% (7days post-administration) and 100% (23 days post-administration) after EPMtreatment at 1 and 1.5 mg\/kg, respectively. EPM administered at 1.5 mg\/kg alsoshowed a significantly higher deleterious effect on tick fertility as measuredby FEI (P &lt; 0.01). Therefore, treatment with EPM may be useful forcontrolling ticks in cattle, particularly in dairy production systems.","palabras_clave":"Eprinomectin|Rhipicephalus (Boophilus) microplus|Cattle|Pharmacokinetic-pharmacodynamic relationship"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4124,"idioma_id":4,"titulo":"Hepatic biotransformation pathways and ruminal metabolic stability of the novel anthelmintic monepantel in sheep and cattle","resumen":"Monepantel (MNP) is a new amino-acetonitrile derivative anthelmintic drugused for the treatment of gastrointestinal (GI) nematodes in sheep. The presentwork investigated the main enzymatic pathways involved in the hepaticbiotransformation of MNP in sheep and cattle. The metabolic stability inruminal fluid of both the parent drug and its main metabolite (monepantelsulphone, MNPSO2) was characterized as well. Additionally, the relative distribution of both anthelmintic molecules between the fluid and particulatephases of the ruminal content was studied. Liver microsomal fractions fromsix (6) rams and five (5) steers were incubated with a 40 lM of MNP. Heatpretreatment (50 °C for 2 min) of liver microsomes was performed for inactivation of the flavin-monooxygenase (FMO) system. Additionally, MNP wasincubated in the presence of 4, 40, and 80 lM of methimazole (MTZ), a FMOinhibitor, or equimolar concentrations of piperonyl butoxide (PBx), a wellknowngeneral cytochrome P450 (CYP) inhibitor. In both ruminant species,MNPSO2 was the main metabolite detected after MNP incubation with livermicrosomes. The conversion rate of MNP into MNPSO2 was fivefold higher(P < 0.05) in sheep (0.15  0.08 nmol\/minmg) compared to cattle. Insheep, the relative involvement of both FMO and CYP systems (FMO\/CYP)was 36\/64. Virtually, only the CYP system appeared to be involved in theproduction of MNPSO2 in cattle liver. Methimazole significantly reduced (41to 79%) the rate of MNPSO2 production in sheep liver microsomes whereas itdid not inhibit MNP oxidation in cattle liver microsomes. On the other hand,PBx inhibited the production of MNPSO2 in liver microsomes of both sheep(58 to 98%, in a dose-dependent manner) and cattle (almost 100%, independentlyof the PBx concentration added). The incubation of MNP and MNPSO2 with ruminal contents of both species showed a high chemical stability without evident metabolism and\/or degradation as well as an extensive degree of adsorption (83% to 90%) to the solid phase of the ruminal content. Overall, these results are a further contribution to the understanding of the metabolicfate of this anthelmintic drug in ruminants.","palabras_clave":"MONEPANTEL|BIIOTRANSFORMATION|RUMINAL STABILITY|RUMINANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4125,"idioma_id":4,"titulo":"A family of generalized quantum entropies: definition and properties","resumen":"We present a quantum version of the generalized (h,&#981;)-entropies, introduced by Salicrú et al. for the study of classical probability distributions. We establish their basic properties and show that already known quantum entropies such as von Neumann, and quantum versions of Rényi, Tsallis, and unified entropies, constitute particular classes of the present general quantum Salicrú form. We exhibit that majorization plays a key role in explaining most of their common features. We give a characterization of the quantum (h,&#981;)-entropies under the action of quantum operations and study their properties for composite systems. We apply these generalized entropies to the problem of detection of quantum entanglement and introduce a discussion on possible generalized conditional entropies as well.","palabras_clave":"QUANTUM ENTROPIES|MAJORIZATION RELATION|ENTANGLEMENT DETECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4127,"idioma_id":4,"titulo":"Alkaloids from Hippeastrum argentinum and Their Cholinesterase- Inhibitory Activities: An in Vitro and in Silico Study","resumen":"Two new alkaloids, 4-O-methylnangustine (1) and 7-hydroxyclivonine (2) (montanine and homolycorine types, respectively), and four known alkaloids were isolated from the bulbs of Hippeastrum argentinum, and their cholinesterase-inhibitory activities were evaluated. These compounds were identified using GC-MS, and their structures were defined by physical data analysis. Compound 2 showed weak butyrylcholinesterase (BuChE)-inhibitory activity, with a half-maximal inhibitory concentration (IC50) value of 67.3 ± 0.09 &#956;M. To better understand the experimental results, a molecular modeling study was also performed. The combination of a docking study, molecular dynamics simulations, and quantum theory of atoms in molecules calculations provides new insight into the molecular interactions of compound 2 with BuChE, which were compared to those of galantamine.","palabras_clave":"Cholinesterase- Inhibitory Activities|galantamine|in Silico Study"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4129,"idioma_id":4,"titulo":"Wind energy potential assessment and techno-economic performance of wind turbines in coastal sites of Buenos Aires province, Argentina","resumen":"An assessment of wind energy potential was carried out in five sites (four onshore and one offshore) in South-West (SW) of Buenos Aires province (Argentina). We use high-resolution wind data (2 and 5 min) for the period 2009?2012. The power law was used to estimate the wind speed at 30, 40, and 60 m height from the anemometer position. Turbulence intensity and wind direction were analyzed. Statistical analyses were conducted using two-parameter Weibull distribution. A techno-economic analysis based on a set of commercial wind turbines was performed in those sites. The results derived from this work indicate that the SW of Buenos Aires province represents a promising area for the wind energy extraction, which would encourage the construction of wind farms for electricity generation.","palabras_clave":"WIND ENERGY|CAPACITY|WINDBULL |COASTAL SITES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4130,"idioma_id":4,"titulo":"Geomorphometric assessment of drainage systems in a semi-arid region of Argentina using geospatial tools and multivariate statistics","resumen":"In semi-arid environments there is often a lack of data on hydrological variables that limits the ability to understand key hydrological processes. In response to this need, geomorphometric analysis is a quantitative approach that has proven to be useful. This work aims to assess and classify 35 exorheic drainage basins located in a semi-arid area of Argentina (Northeastern Patagonia) according to their geomorphometric properties by using GIS technology and principal component (PCA) and cluster analysis (CA) multivariate techniques. In addition, an assessment of automated drainage network extraction accuracy was performed by comparing it with the actual drainage network. The study showed that it was possible to derive automated drainage networks with errors lower than 6 %. By comparing both PCA and CA, it was found that the former allows a good understanding of the clustering of basins from the CA. All basins were clustered into four groups following a significant spatial continuity. This type of study gives the basis for regional-scale analysis, and provides further information for subsequent modeling.","palabras_clave":"GEOMORPHOMETRY|PRINCIPAL COMPONENTS|CLUSTER ANALYSIS|SEMI ARID REGION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4131,"idioma_id":5,"titulo":"Distribución y variabilidad de la precipitación en la Región Pampeana, Argentina","resumen":"La Región Pampeana argentina es el principal centro de producción agropecuario del país y su economía depende, entre otras variables, del régimen de la precipitación. El objetivo del presente estudio fue zonificar esta región considerando la distribución espacio-temporal de la precipitación. Para ello, se aplicó un análisis de conglomerados sobre series mensuales de 33 estaciones meteorológicas pertenecientes al Servicio Meteorológico Nacional (SMN) durante el período 1960-2010. Como resultado se obtuvieron 6 subregiones. Los eventos extremos de precipitación en cada una de estas se estudiaron con el Índice Estandarizado de Precipitación (IEP) y la Transformada de Wavelet Continua (CWT). Se observó que la precipitación responde a variaciones interanuales y estacionales en distintos periodos e intensidad para cada subregión. Sin embargo cuatro de ellas se caracterizaron por presentar homogeneidad pluviométrica, lo que permitió redefinir los límites de la región pampeana propuestos por Labraga et al., 2011. A partir de ello, se confeccionó una nueva regionalización que incorporó a aquellos regímenes pluviométricos pampeanos.","palabras_clave":"Región Pampeana|Variabilidad de las Precipitaciones|Regionalización"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4132,"idioma_id":4,"titulo":"Drainage basin morphometry of the Encadenadas del Oeste lakes, Argentina","resumen":"The Las Encadenadas basin can be defined as an endorheic fluviolacustrine system. The aim of this study is to identify hydrographic sectors and subbasins within the Encadenada?s drainage basin and analyze the former?s morphometric properties including hypsometry. The morphometric analysis allowed for quantification of variables and indices for example area, perimeter, total length of streams, etc. Hypsometric curves were also plotted for each subbasin and finally, principal components analysis was used to sort basins based on results from individually calculated parameters and indices. This  study?s aim was to define for the first time the various drainage subbasins that comprise the Encadenadas del Oeste?s basin. The characterization of these units shows that the basin ismorphologically diverse due to the dynamic fluvial activity that prevails within its limits. One of the above mentioned morphological units are the alluvial cones which form at the mouths of the mainstreams and delineate the bases of the different subbasins. The drainage network exhibits overall a low level of ramification and hierarchy which is likely due to the sedimentary nature and high permeability of the sub-surface soil.","palabras_clave":"BASIN|MORPHOMETRIC|DRAINAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4133,"idioma_id":5,"titulo":"Modificaciones en la distribución espacial de la temperatura y la humedad relativa como resultado del crecimiento urbano: el caso de la ciudad de Bahía Blanca, Argentina","resumen":"El crecimiento de las ciudades genera modificaciones en la atmósfera local que pueden afectar la confortabilidad y calidad de vida de la población. El objetivo del presente trabajo fue comparar la evolución de la isla de calor de verano e invierno entre los años 1985 y 2014 en Bahía Blanca (Argentina). Esta ciudad ha sufrido un importante aumento de la población y de las actividades industriales en los últimos años. Se estudió el aumento de la zona urbana construida, las variaciones espaciales de temperatura y humedad relativa y el crecimiento de la población. Se calcularon índices de confort climático (Humidex, Termohigrométrico y Temperatura Equivalente). La zona urbana construida se extendió más de 110 km2 y el incremento de la población fue de 22,4%. Las modificaciones en el espacio urbano, junto con una mayor densidad de edificios, generaron cambios en las temperaturas de verano de 1985 y 2014. En la primera el centro de la ciudad fue más cálido que las zonas periféricas. La zona más confortable fue la costera. En 2014, en el centro de la ciudad se presentaron las temperaturas más altas y las zonas más confortables fueron las periféricas y los parques. Las mediciones de invierno no registraron diferencias considerables entre sí en las mediciones nocturnas. Los resultados demostraron que el crecimiento de las edificaciones y el incremento de la población de Bahía Blanca modificaron la distribución espacial de la temperatura y la humedad relativa y generaron situaciones de desconfort. Estos resultados generaron información necesaria para futuros planes de desarrollo urbano.","palabras_clave":"ISLA DE CALOR URBANA|CLIMA URBANO|BAHIA BLANCA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4134,"idioma_id":4,"titulo":"SDSS-III Baryon Oscillation Spectroscopic Survey Data Release 12: galaxy target selection and large-scale structure catalogues","resumen":"The Baryon Oscillation Spectroscopic Survey (BOSS), part of the SloanDigital Sky Survey (SDSS) III project, has provided the largest surveyof galaxy redshifts available to date, in terms of both the number ofgalaxy redshifts measured by a single survey, and the effectivecosmological volume covered. Key to analysing the clustering of thesedata to provide cosmological measurements is understanding the detailedproperties of this sample. Potential issues include variations in thetarget catalogue caused by changes either in the targeting algorithm orproperties of the data used, the pattern of spectroscopic observations,the spatial distribution of targets for which redshifts were notobtained, and variations in the target sky density due to observationalsystematics. We document here the target selection algorithms used tocreate the galaxy samples that comprise BOSS. We also present thealgorithms used to create large-scale structure catalogues for the finalData Release (DR12) samples and the associated random catalogues thatquantify the survey mask. The algorithms are an evolution of those usedby the BOSS team to construct catalogues from earlier data, and havebeen designed to accurately quantify the galaxy sample. The code used,designated MKSAMPLE, is released with this paper.","palabras_clave":"COSMOLOGY|OBSERVATIONS|LARGE SCALE STRUCTURE OF THE UNIVERSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4135,"idioma_id":4,"titulo":"WATER POLLUTED WITH GLYPHOSATE FORMULATIONS: EFFECTIVENESS OF A DECONTAMINATION PROCESS USING CHLORELLA VULGARIS GROWING AS BIOINDICATOR","resumen":"The environmental pollution caused by pesticides is considered a major problemworldwide. Glyphosate is one of the herbicides most widely used, and its use hasincreased sharply in the last years. In this work the toxicity of four commercial glyphosate formulations (Eskoba®, Panzer Gold®, Roundup Ultramax® and Sulfosato Touchdown®) was assessed by determining the median effective concentration at 96 h (96 h-EC50) using the microalgae Chlorella vulgaris as the biological model. Although the formulations tested are moderately to slightly toxic to C. vulgaris according to the World Health Organization´s toxicity categories for aquatic and terrestrial organisms, this research shows that the four formulations are toxic, being Eskoba® the least toxic and Roundup Ultramax® the most toxic one. On the other hand, a UV\/H2O2 remediation process for the detoxification of the samples was tested. Its effectiveness was evaluated using a C.vulgaris growth inhibition test. Growth inhibition of C. vulgaris did not reach 18.2 %, indicating the efficacy of the UV\/H2O2 remediation process toreduce glyphosate toxicity. In some of the samples tested within the first 48 h of the assay, C. vulgaris growth was even encouraged. The results of the present work suggest that the selected species was a good indicator to determine glyphosate formulations toxicity level, and shows the relevance of the ecotoxicological tests to evaluate a physicochemical remediation process.","palabras_clave":"GLYPHOSATE FORMULATIONS|UV\/H2O2 PROCESS|ECOTOXICITY|CHLORELLA VULGARIS|BIOINDICATOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4136,"idioma_id":4,"titulo":"Assessment of image features for vessel wall segmentation in intravascular ultrasound images","resumen":"Background Intravascular ultrasound (IVUS) provides axial greyscale images, allowing the assessment of the vessel wall and the surrounding tissues. Several studies have described automatic segmentation of the luminal boundary and themedia?adventitia interface by means of different image features.Purpose The aim of the present study is to evaluate the capability of some of themost relevant state-of-the-art image features for segmenting IVUS images. The study is focused on Volcano 20MHz frames not containing plaque or containingfibrotic plaques, and, in principle, it could not be applied to frames containing shadows, calcified plaques, bifurcations and side vessels.Methods Several image filters, textural descriptors, edge detectors, noise and spatialmeasures were taken into account. The assessment is based on classification techniques previously used for IVUS segmentation, assigning to each pixel a continuous likelihood value obtained using support vectormachines (SVMs). To retrieve relevant features, sequential feature selection was performed guided by the area under the precision?recall curve (AUC-PR).Results Subsets of relevant image features for lumen, plaque and surrounding tissues characterization were obtained, and SVMs trained with these features were able to accurately identify those regions. The experimental results were evaluated with respect to ground truth segmentations from a publicly available dataset, reaching values of AUC-PR up to 0.97 and Jaccard index close to 0.85.Conclusion Noise-reduction filters and Haralick?s textural features denoted their relevance to identify lumen and background. Laws? textural features, local binary patterns, Gabor filters and edge detectors had less relevance in the selection process.","palabras_clave":"IVUS|Vessel wall|Segmentation|Feature selection|SVM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4137,"idioma_id":4,"titulo":"A multilayer network approach for guiding drug repositioning in neglected diseases","resumen":"Drug development for neglected diseases has been historically hampered due to lack of market incentives. The advent of public domain resources containing chemical information from high throughput screenings is changing the landscape of drug discovery for these diseases. In this work we took advantage of data from extensively studied organisms like human, mouse, E. coli and yeast, among others, to develop a novel integrative network model to prioritize and identify candidate drug targets in neglected pathogen proteomes, and bioactive drug-like molecules. We modeled genomic (proteins) and chemical (bioactive compounds) data as a multilayer weighted network graph that takes advantage of  bioactivity data across 221 species, chemical similarities between 1.7 105 compounds  and several functional relations among 1.67 105 proteins. These relations comprised orthology, sharing of protein domains, and shared participation in defined biochemical pathways. We showcase the application of this network graph to the problem of prioritization of new candidate targets, based on the information available in the graph for known compound-target associations.  We validated this strategy by performing a cross validation procedure for known mouse and Trypanosoma cruzi targets and showed that our approach outperforms classic alignment-based approaches.  Moreover, our model provides additional flexibility as two different network definitions could be considered, finding in both cases qualitatively different but sensible candidate targets. We also showcase the application of the network to suggest targets for orphan compounds that are active against Plasmodium falciparum in high-throughput screens. In this case our approach provided a reduced prioritization list of target proteins for the query molecules and showed the ability to propose new testable hypotheses for each compound. Moreover, we found that some predictions highlighted by our network model were supported by independent experimental validations as found post-facto in the literature.","palabras_clave":"COMPLEX NETWORKS|DRUG DISCOVERY|NEGLECTED DISEASES|TARGET PRIORITIZATION|COMPOUND DEORPHANIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4139,"idioma_id":5,"titulo":"Entre volcanes: transformaciones en el ámbito rural patagónico.  Los casos de la Meseta Central (Santa Cruz) y la Línea Sur (Río Negro), 1991-2011.","resumen":"El artículo aborda, en primera instancia, la discusión en torno a la nueva ruralidad, sus principales aportes en América Latina y también el vínculo que mantiene con la noción de territorio y sus sucesivas reformulaciones. Luego, se presentan los dos casos en estudio junto con la descripción de las características socioeconómicas y ambientales que consideramos relevantes para nuestro análisis, procurando revisar la cercanía y la distancia entre aquella producción teórica y la situación contemporánea de dos contextos geográficos particulares de Argentina: la denominada «Línea Sur» de Río Negro y la Meseta Central de Santa Cruz, ambas en la Patagonia. Las fechas que delimitan el lapso de estudio se corresponden con la erupción de los volcanes Hudson (1991) y Puyehue (2011), cuyo impacto en la Meseta Central y en la Línea Sur respectivamente ha sido de especial relevancia. Cerramos el texto con una somera recapitulación y reflexiones.","palabras_clave":"NUEVA RURALIDAD|TERRITORIO|TRANSFORMACIONES|GANADERÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4140,"idioma_id":4,"titulo":"A Note on Bounded Solutions of a Generalized Lienard Type System","resumen":"In this paper we study the boundedness of solutions of some generalized Liénard type system under non usual conditions on evolved functions using the Second Method of Lyapunov.","palabras_clave":"Boundedness|asymptotic behavior |Liénard equation Type"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4141,"idioma_id":5,"titulo":"DISEÑO DE UN TÚNEL DE VIENTO PORTÁTIL PARA EL ESTUDIO DE LA EROSIÓN EÓLICA","resumen":"La erosión eólica (EE) es un proceso de degradación irreversible de suelos de regiones áridas y semiáridas. Con el uso de túneles de viento portátiles es posible simular la EE controlando variables críticas como la velocidad y dirección del viento, la duración de los eventos erosivos; además de parámetros del suelo en condiciones naturales como los niveles de humedad, rugosidad y cobertura. Los objetivos de este trabajo fueron: 1) describir los principales aspectos del diseño y la operación de un túnel de viento portátil para estudios de erosión eólica, y 2) Calibrar el túnel para cumplir con los criterios aerodinámicos relacionados con el desarrollo de un perfil vertical de viento y de distribución de material erosionado similares a las condiciones naturales. Los principales componentes del túnel son: chasis, motor, hélice, sección curva, placa cribada y las secciones del túnel propiamente dicho. Cada una de estas secciones tiene 2 m de largo; 0,5 m de ancho y 1 m de alto. El túnel fue calibrado en un Haplustol Éntico con una superficie lisa y sin cobertura vegetal. La velocidad máxima alcanzada medida a 0,6 m fue de 22,5 m s-1. Esto significa que el túnel permite simular velocidades consideradas erosivas a campo. El perfil vertical de viento y la distribución de material erosionadoson similares a los producidos en condiciones naturales. El túnel de viento desarrollado cumple con las condiciones de diseño para la simulación de eventos de erosión eólica a campo. El uso del mismo mejorará el estudio de procesos básicos y el desarrollo de tecnología para el control de la erosión eólica en suelos de Argentina, además de ser una fuente de consulta para la construcciónde futuros túneles de viento para el estudio de la erosión eólica.","palabras_clave":"METODOLOGÍA DE MEDICIÓN|PERFIL VERTICAL DE VIENTO|DISTRIBUCIÓN VERTICAL DE MATERIAL EROSIONADO POR EL VIENTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4142,"idioma_id":4,"titulo":"Dynamic transition in Landau-Zener-St ¨uckelberg interferometry of dissipative systems: The case of the flux qubit","resumen":"We study Landau-Zener-Stuckelberg (LZS) interferometry in multilevel systems coupled to an Ohmic quantumbath. We consider the case of superconducting flux qubits driven by a dc+ac magnetic fields, but our results canapply to other similar systems. We find a dynamic transition manifested by a symmetry change in the structureof the LZS interference pattern, plotted as a function of ac amplitude and dc detuning. The dynamic transitionis from an LZS pattern with nearly symmetric multiphoton resonances to antisymmetric multiphoton resonancesat long times (above the relaxation time). We also show that the presence of a resonant mode in the quantumbath can impede the dynamic transition when the resonant frequency is of the order of the qubit gap. Our resultsare obtained by a numerical calculation of the finite time and the asymptotic stationary population of the qubitstates, using the Floquet-Markov approach to solve a realistic model of the flux qubit considering up to ten energylevels.","palabras_clave":"qubits"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4143,"idioma_id":5,"titulo":"Intercambio de experiencias productivas sobre quesos artesanales en San Luis Del Palmar, Corrientes","resumen":"Considerando la ancestral tradición dela manufactura quesera en la provincia deCorrientes y el acercamiento de peque-ños productores zonales a la Universidadocurrido en 2014. El Grupo BiMIA realizó,en el año 2015, una Jornada Prácticade elaboración de quesos en San Luis delPalmar, enfatizando las Buenas Prácticasde Manufactura y el uso de insumos tradicionalesa fin de mantener esta usanza,que genera un producto de característicasparticulares y puede ocasionar una mayordemanda, contribuyendo al desarrollosustentable y sostenibilidad productivade la región. Se presenta la experienciay algunos resultados de importancia.","palabras_clave":"queseros artesanos| quesos tradicionales|manufactura|tradición"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4144,"idioma_id":4,"titulo":"Deep electron traps in HfO2-based metal-oxide-semiconductor capacitors","resumen":"Hafnium oxide (HfO2) is currently considered to be a good candidate to take part as a component in charge-trapping nonvolatile memories. In this work, the electric field and time dependences of the electron trapping\/detrapping processes are studied through a constant capacitance voltage transient technique on metal-oxide-semiconductor capacitors with atomic layer deposited HfO2 as insulating layer. A tunneling-based model is proposed to reproduce the experimental results, obtaining fair agreement between experiments and simulations. From the fitting procedure, a band of defects is identified, located in the first 1.7 nm from the Si\/HfO2 interface at an energy level Et = 1.59 eV below the HfO2 conduction band edge with density Nt = 1.36 × 1019 cm&#8722; 3. A simplified analytical version of the model is proposed in order to ease the fitting procedure for the low applied voltage case considered in this work.","palabras_clave":"Hafnium oxide|ALD|Electrical characterization|Electrical Modeling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4145,"idioma_id":4,"titulo":"Analysis of SRAM-Based FPGA SEU Sensitivity to Combined EMI and TID-Imprinted Effects","resumen":"his work proposes a novel methodology to evaluateSRAM-based FPGA?s susceptibility with respect to Single-Event Upset (SEU) as a function of noise on VDD power pins, Total-Ionizing Dose (TID) and TID-imprinted effect on BlockRAM cells.The proposed procedure is demonstrated for SEU measurementson a Xilinx Spartan 3E FPGA operating in an 8 MV Pelletronaccelerator for the SEU test with heavy-ions, whereas TID wasdeposited by means of a Shimadzu XRD-7000 X-ray diffractome-ter. In order to observe the TID-induced imprint effect inside theBlockRAM cells, a second SEU test with neutrons was performedwith Americium\/Beryllium (241 AmBe). The noise was injectedinto the power supply bus according to the IEC 61.000-4-29 stan-dard and consisted of voltage dips with 16.67% and 25% of theFPGA?s VDD at frequencies of 10 Hz and 5 kHz, respectively. At the end of the experiment, the combined SEU failure rate, given inerror\/bit.day, is calculated for the FPGA?s BlockRAM cells. The combined failure rate is defined as the average SEU failure rate  computed before and after exposition of the FPGA to the TID.","palabras_clave":"Combined test|EMI|SEU sensitivity|SRAM-Based FPGA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4146,"idioma_id":4,"titulo":"Particle detection and classification using commercial off the shelf CMOS image sensors","resumen":"In this paper we analyse the response of two different Commercial Off The shelf CMOS image sensors as particle detectors. Sensors were irradiated using X-ray photons, gamma photons, beta particles and alpha particles from diverse sources. The amount of charge produced by different particles, and the size of the spot registered on the sensor are compared, and analysed by an algorithm to classify them. For a known incident energy spectrum, the employed sensors provide a dose resolution lower than microGray, showing their potentials in radioprotection, area monitoring, or medical applications.","palabras_clave":"Radiation detectors|CMOS image sensors|Alpha Particles|Beta rays|Gamma rays|X-rays|Dosimetry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4147,"idioma_id":4,"titulo":"Exposure to a glyphosate-based herbicide during pregnancy and lactation induces neurobehavioral alterations in rat offspring","resumen":"The impact of sub-lethal doses of herbicides on human health and the environment is a matter of controversy. Due to the fact that evidence particularly of the effects of glyphosate on the central nervous system of rat offspring by in utero exposure is scarce, the purpose of the present study was to assess the neurobehavioral effects of chronic exposure to a glyphosate-containing herbicide during pregnancy andlactation. To this end, pregnant Wistar rats were exposed through drinking water to 0.2% or 0.4% of a commercial formulation of glyphosate(corresponding to a concentration of 0.65 or 1.30 g\/L of glyphosate,respectively) during pregnancy and lactation and neurobehavioralalterations in offspring were analyzed. The postnatal day on which eachpup acquired neonatal reflexes (righting, cliff aversion and negativegeotaxis) and that on which eyes and auditory canals were fully openedwere recorded for the assessment of sensorimotor development. Locomotoractivity and anxiety levels were monitored via open field test and plusmaze test, respectively, in 45- and 90-day-old offspring. Pups exposed toa glyphosate-based herbicide showed early onset of cliff aversion reflexand early auditory canal opening. A decrease in locomotor activity and inanxiety levels was also observed in the groups exposed to a glyphosatecontaining herbicide. Findings from the present study reveal that early exposure to a glyphosate-based herbicide affects the central nervous system in rat offspring probably by altering mechanisms orneurotransmitter systems that regulate locomotor activity and anxiety.","palabras_clave":"GLYPHOSATE-BASED HERBICIDES|PERINATAL EXPOSURE|SENSORIMOTOR REFKEXES|LOCOMOTOR ACTIVITY|ANXIETY|RATS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4148,"idioma_id":4,"titulo":"Framework for Modelling and Simulating the Supply Process Monitoring to Detect and Predict Disruptive Events","resumen":"Disruptive events that take place during supply process execution produce negative effects that propagate throughout a supply chain. Event management systems for supply chains have emerged to provide functionality for monitoring schedules, managing disruption, and repairing schedules affected by a disruptive event. A Web service that provides a schedule monitoring functionality for supply chain event management was developed. This paper provides a framework to allow enterprises that hire this service to develop simulation models of monitoring processes and evaluate their ability to detect and anticipate disruptive events. The framework, based on discrete event simulation, is implemented in a library that can be used for developing and testing monitoring processes by means of a friendly interface. A marine freight transport process was used as a case study to show how a supply process and its environment can be modelled and simulated by using the library. Simulation results show the ability of this approach to anticipate disruptive events and identify critical stages of a supply process in order to prevent disruptive events.","palabras_clave":"SCEM system |Monitoring system|Disruptive event|Simulation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4149,"idioma_id":4,"titulo":"Epigenetic activation of Sox2 gene in the developing vertebrate neural plate","resumen":"One of the earliest manifestations of neural inductionis onset of expression of the neural marker Sox2,mediated by the activation of the enhancers N-1 and N-2. By using loss andgain-of-function we find that Sox2 expressionrequires the activity of JmjD2A andthe Msk1 kinase, which can respectivelydemethylate the repressive H3K9me3 mark and phosphorylate the activating H3S10(H3S10ph) mark. Bimolecular fluorescence complementation reveals that the adaptorprotein 14-3-3, known to bind to H3S10ph, interacts with JMJD2A and may beinvolved in its recruitment to regulatory regions of Sox2 gene. Chromatin immunoprecipitation reveals dynamic binding ofJMJD2A to the Sox2 promoter and N-1enhancer at the time of neural plate induction. Finally, we show a cleartemporal antagonism on the occupancy of H3K9me3 and H3S10ph modifications atthe promoter of Sox2 locus before and after the neural plate induction. Taken together,we propose a series of epigenetic events necessary for the early activation of theSox2 gene in neural progenitor cells.","palabras_clave":"Epigenetics|neural plate|sox2|jmjd2a"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4150,"idioma_id":4,"titulo":"Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)","resumen":"In 2008 we published the first set of guidelines for standardizingresearch in autophagy. Since then, research on this topichas continued to accelerate, and many new scientists haveentered the field. Our knowledge base and relevant new tech-nologies have also been expanding. Accordingly, it is importantto update these guidelines for monitoring autophagy in differentorganisms. Various reviews have described the range ofassays that have been used for this purpose. Nevertheless, therecontinues to be confusion regarding acceptable methods to2measure autophagy, especially in multicellular eukaryotes.","palabras_clave":"Autophagy|Nomenclature|Eukaryotes|ATG8"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4151,"idioma_id":4,"titulo":"Hydroxylation and translational adaptation to stress: some answers lie beyond the STOP codon","resumen":"Regulation of protein synthesis contributes to maintenance of homeostasis and adaption to environmental changes. mRNA translation is controlled at various levels including initiation, elongation and termination, through post-transcriptional\/translational modifications of components of the protein synthesis machinery. Recently, protein and RNA hydroxylation has emerged as an important enzymatic modification of tRNAs, elongation and termination factors, as well as ribosomal proteins. This modification, catalyzed by 2-oxoglutarate dependent oxygenases, can lead to increased STOP codon read-through, presumably contributing to adaptation of the cell to stress conditions.","palabras_clave":"Protein synthesis|Hydroxylation|Adaptation to stress|STOP-codon read through"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4152,"idioma_id":4,"titulo":"Musashi mediates translational repression of the Drosophila hypoxia inducible factor","resumen":"Adaptation to hypoxia depends on a conserved &#945;\/&#946; heterodimeric transcription factor called Hypoxia Inducible Factor (HIF), whose &#945;-subunit is regulated by oxygen through different concurrent mechanisms. In this study, we have identified the RNA binding protein dMusashi, as a negative regulator of the fly HIF homologue Sima. Genetic interaction assays suggested that dMusashi participates of the HIF pathway, and molecular studies carried out in Drosophila cell cultures showed that dMusashi recognizes a Musashi Binding Element in the 3´ UTR of the HIF&#945; transcript, thereby mediating its translational repression in normoxia. In hypoxic conditions dMusashi is downregulated, lifting HIF&#945; repression and contributing to trigger HIF-dependent gene expression. Analysis performed in mouse brains revealed that murine Msi1 protein physically interacts with HIF-1&#945; transcript, suggesting that the regulation of HIF by Msi might be conserved in mammalian systems. Thus, Musashi is a novel regulator of HIF that inhibits responses to hypoxia specifically when oxygen is available.","palabras_clave":"Musashi|Hypoxia|Sima|Hypoxia Inducible Factor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4153,"idioma_id":4,"titulo":"PIP Water Transport and Its pH Dependence Are Regulated by Tetramer Stoichiometry","resumen":"Many plasma membrane channels form oligomeric assemblies, and heterooligomerization has been described as a distinctive feature of some protein families. In the particular case of plant plasma membrane aquaporins (PIPs), PIP1 and PIP2 monomers interact to form heterotetramers. However, the biological properties of the different heterotetrameric configurations formed by PIP1 and PIP2 subunits have not been addressed yet. Upon coexpression of tandem PIP2-PIP1 dimers in Xenopus oocytes, we can address, for the first time to our knowledge, the functional properties of single heterotetrameric species having 2:2 stoichiometry. We have also coexpressed PIP2-PIP1 dimers with PIP1 and PIP2 monomers to experimentally investigate the localization and biological activity of each tetrameric assembly. Our results show that PIP2-PIP1 heterotetramers can assemble with 3:1, 1:3, or 2:2 stoichiometry, depending on PIP1 and PIP2 relative expression in the cell. All PIP2-PIP1 heterotetrameric species localize at the plasma membrane and present the same water transport capacity. Furthermore, the contribution of any heterotetrameric assembly to the total water transport through the plasma membrane doubles the contribution of PIP2 homotetramers. Our results also indicate that plasma membrane water transport can be modulated by the coexistence of different tetrameric species and by intracellular pH. Moreover, all the tetrameric species present similar cooperativity behavior for proton sensing. These findings throw light on the functional properties of PIP tetramers, showing that they have flexible stoichiometry dependent on the quantity of PIP1 and PIP2 molecules available. This represents, to our knowledge, a novel regulatory mechanism to adjust water transport across the plasma membrane.","palabras_clave":"aquaporins|membrane proteins|oligomerization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4154,"idioma_id":5,"titulo":"Bioarqueología y prácticas mortuorias de valles mesotermales del oeste catamarqueño, Argentina: resultados preliminares","resumen":"Se describen y discuten los primeros resultados de los análisis bioarqueológicos de once inhumaciones realizadas en los valles de Fiambalá (Depto. Tinogasta), Hualfín (Depto. Belén) y Andalgalá (Depto. Andalgalá) de la provincia de Catamarca, Argentina. La muestra, recuperada en el marco de proyectos de investigación (Fiambalá) y de estudios de impacto (Hualfín y Andalgalá), se ubica temporalmente entre los siglos VI y XV d.C. y presenta amplia diversidad bioarqueológica. El objetivo es recuperar información de los contextos mortuorios, realizar una síntesis de los antecedentes regionales publicados e integrar ambos conjuntos de datos para los tres valles catamarqueños. Se presentan las principales tendencias identificadas, que dan cuenta de similitudes y diferencias en el comportamiento mortuorio, la salud y el estilo de vida de los individuos analizados.","palabras_clave":"BIOARQUEOLOGÍA|PRACTICAS MORTUORIAS|TENDENCIAS TEMPORALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4155,"idioma_id":4,"titulo":"Land allocation among the main crops in Argentina: Estimation of price and land elasticities","resumen":"In the past twenty years, there have been significant changes in land allocation among the main crops in Argentina. The aim of this paper was to analyze how those changes responded to variations in their relative prices in the short-term and quantify theirresponses. Additionally, it focused on understanding how the hectares devoted to aparticular crop responded to changes in the total planted area. For this purpose a system ofland allocation equations was estimated for the period 1980-2014. The results showed thatthe area planted with corn, soybean, sunflower and wheat responded to their own prices,being inelastic in all cases. A statistically significant and negative cross-price elasticity wasobserved for sunflower and corn. The other cross-price elasticities showed substitution forland use but they were not statistically significant. The land elasticities revealed to be elasticfor wheat and inelastic for corn, soybean and sunflowers, while not significant for sorghum.","palabras_clave":"Differential Approach|Rotterdam Model|Agricultural production|Soybean"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4157,"idioma_id":4,"titulo":"Optical approach for the efficient data volume handling in experimentally encrypted data","resumen":"Experimental optical procedures generate a tremendous amount of data, which must be then processed for any practical application. We present a new optical approach in terms of the data volume for the efficient handling of multiple data obtained from an experimental cryptosystem. In order to achieve this goal, we use the combination of optical filtering and optical scaling of the experimentally registered data. The intention is to reduce by optical means the amount of data to be managed. We define a so called ?efficiency factor? to describe the effectiveness of the approach. We find that volume reduction depends on this factor and the number of objects to be processed. We achieved substantial data volume reductions up to 94.24%. We introduce the basic concepts as well as experimental results that support both the feasibility and the applicability of our approach.","palabras_clave":"encrypted data|volume reduction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4158,"idioma_id":5,"titulo":"Género e innovación en la producción agrícola de baja escala","resumen":"La innovación tecnológica es un aspecto significativo del desarrollo y el mejoramiento productivo que se constituye a partir de elementos socio-técnicos y culturales. Desde la sociología de la innovación se ha señalado una fuerte impronta contextual de los procesos de innovación que refuerza el peso de las características locales de agentes e instituciones intervinientes. A su vez, los estudios feministas sobre la tecnología han identificado y conceptualizado las dimensiones de género presentes en la producción, difusión y apropiación social de nuevos conocimientos inscriptos en diseños y artefactos tecnológicos. Sobre la base de estas tesis generales sobre el carácter social de los procesos de innovación, en este artículo se presenta un esquema conceptual para abordar la situación de género en contextos específicos: la producción agrícola de baja escala. Es necesario ampliar el acervo teórico provisto por los estudios CTS e incluir otras fuentes teóricas desarrolladas en el campo de estudios rurales y los estudios de género, para poder caracterizar apropiadamente fenómenos de difusión de nuevas tecnologías agrícolas en actividades productivas que llevan adelante comunidades pequeñas, de baja inserción en los mercados y de alta vulnerabilidad social. Un aspecto de particular importancia en esta dirección es construir la idea de ?usuarias? y ?usuarios? como participantes en procesos de innovación e identificar la diversidad de escenarios de uso y apropiación de nuevas tecnologías. Esta propuesta teórica ha sido testeada en una reciente investigación sobre procesos de innovación tecnológica en la agricultura familiar algodonera en la Argentina.","palabras_clave":"genero|innovacion|agricultura |algodon"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4159,"idioma_id":5,"titulo":"Representaciones sociales de los personajes de la historia universal: posicionamientos diferenciales en función de la ideología, religiosidad y nacionalismo en población general Argentina","resumen":"Con el objetivo de analizar las Representaciones Sociales de la historia través de los sentimientos que despiertan 24 personajes de la historia universal se administró una versión modificada del cuestionario construido por Liu et al. (2005) a una muestra intencional (N=369) de Argentina (55,7% mujeres, edad M=36,20, DE=11,16).  El análisis de conglomerado no jerárquico arroja cuatro grupos en función de la religiosidad, el nacionalismo y el posicionamiento ideológico de los participantes.  Por su parte, del análisis de cluster jerárquico en función de los sentimientos hacia los personajes se desprenden dos conglomerados denominados  héroes y villanos. Los personajes religiosos y políticos son los que mayor disenso suscitan.","palabras_clave":"HISTORIA|PERSONAJES|UNIVERSAL|REPRESENTACIONES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4160,"idioma_id":4,"titulo":"Characterization of waxes and residual oil recovered from sunflower oil winterization waste","resumen":"Two filter cakes from the sunflower oil winterization process were used to recover, fractionate and characterize their oil and wax fractions. Both samples presented high lipid content (75.35 and 58.21% dry basis) composed of 88.1 and 89.1% oil and 11.9 and 8.9% waxes. Recovered oils had a very low free fatty acid content, differences in the degree of deterioration consistent with their fatty acid composition, and significant differences in their wax contents, which were in the order of crude sunflower oils (1356-604 mg\/kg). Purified waxes were between 40 and 60 carbon atoms with a fatty alcohol and fatty acid distribution in the range of 18-34 and 14-34 carbon atoms, respectively. Significant differences were found between samples in their wax and fatty acid profiles according to differences between the hybrids studied. DSC thermograms presented differences in the onset temperature (71.98 °C and 75.15 °C), melting peak (76.70 and 80.53 °C) and melting enthalpies (202.77 J\/g and 204.35 J\/g), with the lower values being exhibited by the sample with higher fatty acid unsaturation and lower content of waxes with more carbon atoms.","palabras_clave":"Sunflower waxes|filter cake|sunflower oil recovered|winterization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4162,"idioma_id":5,"titulo":"Patrones espaciales en comunidades de macroinvertebrados acuáticos de la Puna Argentina","resumen":"Los macroinvertebrados son un componente vital en los ecosistemas acuáticos, procesando materia orgánica y sirviendo de alimento para otros organismos como los peces o y anfibios. Se realizó la primera descripción cuantitativa de los patrones espaciales de diversidad y composición de macroinvertebrados acuáticos en siete vegas de la Puna Argentina, y su relación con las características del ambiente, de la vegetación circundante y con características físico - químicas del agua. Se ordenaron losLos sitios de muestreo de cada vega fueron ordenados por medio de un NMDS (Non-Metrical Multidimensional Scaling). Se colectaron 3131 individuos de macroinvertebrados acuáticos. Se,los cuales fueron identificaron identificados en 25 taxa pertenecientes a 22 familias. Entre las Se registraron un total de 62 especies de plantas vasculares se registraron un total de 62 especies, pertenecientes a 20 familias. La forma de vida más abundante en generalentre ellas fueron las graminoides cespitosas con un promedio de  30%, seguida por cojines y tapetes con 25%","palabras_clave":"MACROINVERTEBRADOS|VEGAS|VEGETACIÓN|AGUA|PUNA|NEOTROPICAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4163,"idioma_id":5,"titulo":"?Hidroecosistemas? de la Puna y Altos Andes de Argentina","resumen":"Los humedales son zonas en donde el agua es el principal factor que controla el ambiente así como biodiversidad asociada. Especialmente en zonas áridas como la Puna, estos sistemas son de enorme importancia para la biodiversidad y las poblaciones locales que dependen de los servicios ecosistémicos que éstos proveen. En éste trabajo presentamos un mapeo basado en clasificación de imágenes satelitales de los principales humedales de un área de 14.3 millones de ha de la Puna argentina: salares, lagos y lagunas y vegas. En base a esa información cuantificamos y caracterizamos éstos sistemas desde una perspectiva regional de uso del territorio y conservación. Nuestro análisis reporta 866.580 ha de humedales repartidos en 654.076 ha de  salares (4,6% de la superficie del área de estudio), 40.486 ha de cuerpos de agua (0,28%), 61.123 ha de vega salitrosa (0,43%) y 110.895 ha de vegas (0.78%). A su vez la unión de áreas de diferentes clases colindantes resultó en 15 complejos de ?hidroecosistemas? que totalizan 634.851 ha, los cuales cuentan con zonas de transición entre clases que consideramos fluctuarán entre una y otra de acuerdo a la respuesta de los diferentes humedales a cambios climáticos estacionales y\/opresiones antrópicas. Consideramos que la información básica del inventario de humedales y la consideración de los mismos como sistemas complejos funcionales en ?hidroecosistemas? son un aporte relevante a la conservación  y manejo de estos ecosistemas de alto valor ecológico, económico, social y cultural.","palabras_clave":"vegas|salares|lagunas|puna"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4164,"idioma_id":4,"titulo":"Analysis of Epstein Barr virus infection models in a series of pediatric carriers from a developing country","resumen":"Epstein-Barr virus (EBV) is a B lymphotropic human herpesvirus. Two models, germinal center (GC)and direct infection, describe how EBV infects B-cells. Since in Argentina primary infection is mostlysubclinical at young ages, children represent an interesting population where to analyze EBV infection,especially considering that most studies are usually performed in adults. Tonsil biopsies from pediatriccarriers were studied to describe infection characteristics. EBV+ lymphocytes at the interfollicularregion were mainly observed. Latency III pattern in subepithelial (SubEp) lymphocytes was observedat young ages, probably indicating a recent infection. In older patients EBV was mostly detectedin epithelial cells, suggesting that they could have been infected some time ago. This finding wassustained by tonsillar viral load, which was higher in cases with LMP1+SubEp cells vs. LMP1+nonSubEpcells (p=0.0237, Mann-Whiney test). Latency III was prevalent and related to the GC, while latency IIwas associated with non-GC (p=0.0159, &#967;2 test). EBERs+\/IgD+ cells were statistically prevalent overEBERs+\/CD27+ cells (p=0.0021, &#967;2 test). These findings indicated that both EBV infection models arenot mutually exclusive and provide some basis for further understanding of EBV infection dynamics.Moreover, we provide a more accurate explanation of EBV infection in pediatric asymptomatic carriersfrom a developing country","palabras_clave":"EPSTEIN BARR VIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4165,"idioma_id":4,"titulo":"Essential oils and their combinations with iprodione fungicide as potential antifungal agents against withe rot (Sclerotium cepivorum Berk) in garlic (Allium sativum L.) crops","resumen":"Abstract This study was conducted to evaluate essential oils (EOs) and their binary combinations with fungicide iprodione (Ip) as a potential fungicide against Sclerotium cepivorum Berk in order to reduce the amount of pesticide applied in garlic crops. Five essential oils (EOs) from different plants grown in Argentina were studied: Tagetes minuta L. (Su), Tagetes filifolia L. (An), Origanum vulgare L. spp. Vulgare (OCom), Origanum x majoricum (OMen), and Laurus nobilis L. (Ba). The chemical composition of EOs was analyzed by GC?MS. The minimum fungicide concentration (MFC) and minimum inhibitory concentration (MIC) were determined for each compound. A total of 84 combinations of EOs and Ip were assessed looking for synergistic interactions. A phytotoxicity assay was carried out to identify EOs with a potential negative effect on garlic. The effect of EOs, Ip and mixtures on white rot was evaluated in a field study. Fungicide Ip had the best antifungal activity in the in vitro test. OMen and OCom showed high antifungal activity, but also had a strong phytotoxic effect on garlic plants. Su had a moderate antifungal effect with distinguished synergism with Ip, but none can be used due to its phytotoxic effect. An and Ba both had moderate antifungal activity. These EOs had no negative effect on garlic germination and production, being non-phytotoxic for this crop. In this study, the combinations of An and Ba them with Ip have synergistic interactions. Sclerotia density and disease incidence were reduced by these mixtures in a field study. In conclusion, An and Su could be used as natural fungicides to control white rot in garlic, reducing the fungicide dose required. However, future studies are needed to adjust final concentrations to be applied in field crops.","palabras_clave":"NATURAL CONTROL|SYNERGISM|FUNGICIDE|PHYTOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4166,"idioma_id":4,"titulo":"Proteome analysis of Bordetella pertussis isolated from human macrophages","resumen":"Previous studies have shown that B. pertussis survives inside human macrophages in non-acidic compartments with characteristics of early endosomes. In order to gain new insight into the biology of B. pertussis survival in host cells,we haveanalyzed the adaptationof thebacterialproteomeduring intracellular infection.The proteome of B. pertussis 3 h and 48 h after infection of human macrophage-like THP-1 cells was examined by nano-liquid chromatography combined with tandem MS and compared to the protein profile of extracellular B. pertussis growing in the same cell culture medium. Compared with extracellular bacteria, almost 300 proteins out of 762 identified proteins displayed altered levels in intracellular B. pertussis. Functional analyses of the proteins displaying altered abundance revealed enrichment of proteins involved in stress response, iron uptake, cellular metabolism, transcriptional regulation, and virulence. To our knowledge, this is the first analysis of the B. pertussis proteomeduring adaptation to the intramacrophage environment and the data provide new clues for understanding B. pertussis adaptation and pathogenesis. Biological significance: B. pertussis is a respiratory pathogen that has adapted exclusively to the human host. De- spite high vaccination rates, whooping cough remains a serious threat to human health and its incidence has been increasing in recent years in vaccinated populations. Themechanismsthat allowthis pathogen to evade im- mune clearance, persist in the host, and cause a prolonged paroxysmal cough are still poorly understood. Recent studies regarding B. pertussis survival inside host cells and the cellular response to this bacterial infection indicate that B. pertussis may have an intracellular phase during infectionwhich probably contributes to persistence and vaccine failure. In this study we provide the first global proteome profile of B. pertussiswithin macrophages. The data provide novel insights into the adaptive responses elicited by these bacteria for physiological adaptation to the host environment.","palabras_clave":"BORDETELLA PERTUSSIS|INTRACELLULAR SURVIVAL|PROTEOMICS|HOST PATHOGEN INTERACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4167,"idioma_id":4,"titulo":"An insight on acyl migration in solvent-free ethanolysis of model triglycerides using Novozym 435","resumen":"this work, the ethanolysis of triglycerides catalyzed by immobilized lipase was studied,focusing on the secondary reaction of acyl migration. The catalytic tests were performed in asolvent-free reaction medium using Novozym 435 as biocatalyst.The selected experimental variables were biocatalyst loading (5-20 mg), reaction time (30-90min), and chain length of the fatty acids in triglycerides with and without unsaturation (short(triacetin), medium (tricaprylin) and long (tripalmitin\/triolein)).The formation of 2-monoglyceride by ethanolysis of triglycerides was favored by longreaction times and large biocatalyst loading with saturated short- to medium-chain triglycerides.In the case of long-chain triglycerides, the formation of this monoglyceride was widely limitedby acyl migration.In turn, acyl migration increased the yield of ethyl esters and minimized the content ofmonoglycerides and diglycerides. Thus, the enzymatic synthesis of biodiesel was favored bylong-chain triglycerides (which favor the acyl migration), long reaction times and largebiocatalyst loading.The conversion of acylglycerides made from long-chain fatty acids with unsaturation wasrelatively low due to limitations in their access to the active site of the lipase.","palabras_clave":"ETANÓLISIS|TRIGLICÉRIDOS|MIGRACIÓN DE ACILOS|NOVOZYM 435"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4168,"idioma_id":4,"titulo":"In vitro digestibility and pasting properties of epichlorohydrin modified cassava starch","resumen":"The objective of this work was to analyze the influence of epichlorohydrin concentration and reaction time on the food-grade resistant starch production and its pasting properties by using native cassava starch from Misiones-Argentina origin. Cassava starch was modified using epichlorohydrin (0.30% and 0.15%) during 4 or 8 h. Digestibility was evaluated by determining resistant starch as total dietary fiber. Pasting properties and the cross-linking degree were studied using a micro-viscoamylograph Brabender. Resistant starch content was not influenced by epichlorohydrin concentration and reaction time. Cross-linking was detected at higher reaction times (8 h) and epichlorohydrin concentrations (0.30%), where a decrease in viscosity peaks by more than 80% was observed. Both pasting temperature and breakdown were increased, while a decrease in retrogradation was detected. Starches can be suitable for different food applications. This is because of the ability to modify its pasting properties and the invariability of the in vitro digestibility of cassava starch as a result of using epichlorohydrin (at concentrations approved by local and regional legislation) and reaction times of 4 and 8 h. Information related to the modification of cassava starch using epichlorohydrin is scarce or not available nowadays in literature.","palabras_clave":"CASSAVA STARCH|CROSS-LINKING|EPICHLOROHYDRIN|RESISTANT STARCH|PASTING PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4169,"idioma_id":4,"titulo":"Histomorphological changes in testes of broad-snouted caimans (Caiman latirostris) associated with in ovo exposure to endocrine-disrupting chemicals","resumen":"Studies regarding the effects of endocrine-disrupting chemicals (EDCs) on the reproductive functions of wild animals have raised increasing concern. Thus, here we evaluated the consequences of in ovo exposure to endosulfan (END) and bisphenol A (BPA) in testes from neonatal to juvenile (Juv) caimans (Caiman latirostris). Caiman eggs were collected from areas with low to moderate anthropogenic intervention and incubated at male-producing temperature. At stage 20 of embryonic development (previous to gonad sex determination), eggs were exposed to either END (20&#8201;ppm) or BPA (1.4&#8201;ppm) and male gonad histomorphology examined in 10-day-old, 90-day-old, and Juv caimans. The relative seminiferous tubular area (RTA) was measured in testes and the proliferation index and the expression of estrogen receptor alpha (ER&#945;) were quantified in intratubular cells. Regardless of the treatment, all eggs resulted in male hatchlings. The testes of EDC-exposed caimans presented tortuous seminiferous tubules with empty tubular lumens. The RTA of 10-day-old caimans exposed to BPA was decreased. The percentage of cells expressing ER&#945; was not different after in ovo treatment with EDCs (compared to the Control group), although caimans exposed to END showed a different ER&#945; distribution pattern. The proliferation index was lower in 90-day-old caimans exposed to END, and higher in Juv caimans exposed to BPA. In ovo exposure to END or BPA modified sensitive parameters of C. latirostris male gonads. The alterations described here might compromise not only the sexual maturation but also the reproductive performance of adult caimans.","palabras_clave":"Testes|Caimans|EDCs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4170,"idioma_id":4,"titulo":"The presence of synthetic acaricides in beeswax and its influence on the development of resistance in Varroa destructor","resumen":"After honey production, beeswax ranks second as regards hive product used in industry and cosmetics. In Argentina, the use of commercial wax adulterated with paraffin and other olefins for comb foundation is a common practice. As regards beehives, the progressive accumulation of synthetic acaricides in wax has caused adverse effects on bees, mainly on individuals at different stages of development. Another issue associated with the use of synthetic acaricides is the phenomenon of resistance. This study aimed to determine the presence of adulterants and acaricide residues in commercial wax used in Argentina. Furthermore, the relationship between coumaphos content in wax and the development of mite resistance reported in recent years in Argentina was investigated. The results demonstrate that paraffin is the most common contaminant substance present in recycled beeswax and commercial wax used for comb foundation in the country. Coumaphos was also found to be the most common acaricide present in wax; 87% in  commercial and 80% in recycled wax. Fluvalinate was detected in 33% of commercial wax samples and in 27% of recycled wax. A relationship between coumaphos residues and resistance was also established. Future studies should be conducted to establish the mechanisms by which the buildup of acaricides in the beeswax affects the development of resistance in populations of varroa. Presencia de residuos de acaricidas en cera de abejas y su influencia sobre el desarrollo de resistencia de Varroa destructor","palabras_clave":"APICULTURA|RESIDUOS ACARICIDAS|RESISTENCIA|VARROA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4171,"idioma_id":4,"titulo":"Honeybee health in South America","resumen":"Honeybees are essential components to modern agriculture and economy. However, a continuous increase in cases of colony losses and colony depopulation are being reported worldwide. This critical situation has put the fragile equilibrium between bees and plants on the edge. As a consequence, several scientists have begun to focus their lines of research on this issue. Most researchers agree that there is no single explanation for the observed colony losses. Instead, these losses result from a synergistic interaction between different stressors. South America is not the exception; several cases of colony losses and colony depopulation were reported by beekeepers throughout the continent, yet no accurate data has been published to date. Therefore, this article attempts to analyze the past and present situation of honeybee health in South America, specifically in Argentina, Chile, Uruguay, Brazil, and Venezuela. Furthermore, it is intended to serve as a comparison to future colony losses, as well as to provide guidance for future hypothesis-driven research on the causes of colony mortality. We evaluate the impact of the main parasites and pathogens affecting honeybee colonies and discuss the role of each with respect to reported honeybee losses. We also contemplate the main challenges that each nation must confront with regards to honeybee health.","palabras_clave":"APIS MELLIFERA|COLONY LOSSES|HEALTH|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4173,"idioma_id":5,"titulo":"PRESENCIA DE INFECCIÓN POR Trypanosoma cruzi Y Toxoplasma gondii EN PERROS DOMÉSTICOS DE LOCALIDADES RURALES EN EL NOROESTE ARGENTINO","resumen":"Las zoonosis causadas por los parásitos Trypanosoma cruzi y Toxoplasma gondii son prevalentes en la Argentina y los perros cumplen el papel de reservorio además de padecer las enfermedades.Se considera una buena alternativa conocer la seroprevalencia de estas zoonosis en los perros debido a que son un reflejo de lo que ocurre en la población humana y en el ambiente. Nos propusimos conocer la seroprevalencia de infección por T. gondii y T. cruzi en caninos en cuatro zonas rurales de la provincia de Salta. Para la detección de anticuerpos anti-T. gondii se utilizó hemaglutinación Indirecta (HAI) y para la detección de anticuerpos anti-T. cruzi, además de HAI, se usó Enzimoinmunoanálisis (ELISA). Fueron muestreados 209 canes y los resultados serológicos indicaron la presencia de anticuerpos anti-T. gondii en la localidad de La Unión,departamento Rivadavia en 18,2% (4\/22), en San Carlos con 20,5% (8\/39), en Cafayate con 13,1 % (11\/84) y en Embarcación departamento General San Martín con 23.4% (15\/64). Losanticuerpos anti-T cruzi sólo estuvieron presentes en los perros de la localidad de San Carlos con 28,2% (11\/39). La frecuente seroprevalencia a T. gondii, encontrada en los perros de todas las localidades muestreadas sugiere contaminación ambiental por este parásito en la zona. El hallazgo de perros infectados por T. cruzi, en la localidad de San Carlos, nos sugiere la posibilidad de transmisión activa del parásito en un pasado reciente y nos alerta sobre la posibilidad de reservorio.","palabras_clave":"PERROS|ZOONOSIS|SALTA|CHAGAS|TOXOPLASMOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4174,"idioma_id":4,"titulo":"Pharmacological characterization of the nociceptin\/orphanin FQ receptor on ethanol-mediated motivational effects in infant and adolescent rats","resumen":"Activation of nociceptin\/orphanin FQ (NOP) receptors attenuates ethanol drinking and prevents relapse in adult rodents. In younger rodents (i.e., infant rats) activation of NOP receptors blocks ethanol-induced locomotor activation but does not attenuate ethanol intake. The aim of the present study was to extend the analysis of NOP modulation of ethanol?s effects during early ontogeny. Aversive and anxiolytic effects of ethanol were measured in infant and adolescent rats via conditioned taste aversion and the light-dark box test; whereas ethanol-induced locomotor activity and ethanol intake was measured in adolescents only. Before these tests, infant rats were treated with the natural ligand of NOP receptors, nociceptin (0.0, 0.5 or 1.0 µg) and adolescent rats were treated with the specific agonist Ro 64-6198 (0.0, 0.1 or 0.3 mg\/Kg). The activation of NOP receptors attenuated ethanol-induced anxiolysis in adolescents only, and had no effect on ethanol?s aversive effects. Administration of Ro 64-6198 blocked ethanol-induced locomotor activation but did not modify ethanol intake patterns. The attenuation of ethanol stimulating and anxiolytic effect by activation of NOP receptors indicates a modulatory role of this receptor on ethanol effects, which is expressed early in ontogeny.","palabras_clave":"Ethanol anxiolytic effects|ethanol-induced locomotor activation|early ontogeny|NOP opioid receptor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4175,"idioma_id":4,"titulo":"Age-related effects of chronic restraint stress on ethanol drinking, ethanol-induced sedation, and on basal and stress-induced anxiety response","resumen":"Adolescents are sensitive to the anxiolytic effect of ethanol, and evidence suggests that they may be more sensitive to stress than adults. Relatively little is known, however, about age-related differences in stress modulation of ethanol drinking or stress modulation of ethanol-induced sedation and hypnosis. We observed that chronic restraint stress transiently exacerbated free-choice ethanol drinking in adolescent, but not in adult, rats. Restraint stress altered exploration patterns of a light-dark box apparatus in adolescents and adults. Stressed animals spent significantly more time in the white area of the maze and made significantly more transfers between compartments than their non-stressed peers. Behavioral response to acute stress, on the other hand, was modulated by prior restraint stress only in adults. Adolescents, unlike adults, exhibited ethanol-induced motor stimulation in an open field. Stress increased the duration of loss of the righting reflex after a high ethanol dose, yet this effect was similar at both ages. Ethanol-induced sleep time was much higher in adult than in adolescent rats, yet stress diminished ethanol-induced sleep time only in adults. The study indicates age-related differences that may increase the risk for initiation and escalation in alcohol drinking.","palabras_clave":"restraint stress|rat|ethanol| adolescent|adult"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4176,"idioma_id":4,"titulo":"Corrosion protection assessment of nanoparticulate pre-treated galvanized steel\/paint systems subjected to accelerated tests","resumen":"The development of environmentally friendly anticorrosive systems providingthe same performance than those already existing has been the great challengefor researchers working in the surface treatment field. In this work thecorrosion behavior of two electrogalvanized-steel\/nanoparticulate pretreatment\/organiccoating systems exposed to salt spray, controlled humidity and temperature,Prohesion or weathering chambers was studied. The global analysis of theobtained results allowed concluding that the protective behavior of both duplexsystems was satisfactory, and that the nanoparticulate pretreatments based ontrivalent chromium (Cr3+) and Zirconium could be considered as a validalternative to substitute those based on hexavalent chromium(Cr6+).&#61472;","palabras_clave":"ELECTROGALVANIZED STEEL|CONVERSION COATINGS|DUPLEX SYSTEMS|CORROSION|PAINT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4177,"idioma_id":4,"titulo":"STUDY OF SILANE LAYERS GROWN ON STEEL AND CHARACTERIZED USING ELLIPSOMETRY AT DIFFERENT WAVELENGHTS AND INCIDENCE ANGLES.","resumen":"Films of gmercap to propyltrimethoxysilane are prepared by hydrolysis, condensationand curing at 80 oC. The optical indices, n, kand the thickness d are calculated using theellipsometry technique.Aprogramme is developed to fit a wide set of ellipsometric&#61508; and&#61529; data in the visible optical region 400 nm &lt; ë &lt; 600 nm. An increase in the optical absorption k is detected for the lower concentration ofMPTMS attributed to light absorption from the pores","palabras_clave":"MERCAPTOPROPYLTRIMETHOXYSILANE|ELLIPSOMETRY|ANTICORROSIVE COATING|OPTICAL CONSTANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4178,"idioma_id":5,"titulo":"Conectivismo: Hacia El Nuevo Paradigma De La Enseñanza Por Competencias","resumen":"En los últimos años la tecnología hareorganizado la forma en la que vivimos, nos comunicamos y aprendemos.La aparición de la web 2.0 ha aumentado considerablemente el acceso de losestudiantes a la información interconectada que demanda su formaciónprofesional. El progreso científico-tecnológico ha impulsado la innovacióncomo paradigma asertivo de los bienintencionados esfuerzos de desarrolloque responden a los cuestionamientos de una sociedad del conocimiento enconstante transformación. Uno de los grandes retos de las institucioneseducativas consiste en formar profesionales competentes capacitados paraadaptarse a los incesantes cambios y desafíos que propone cada disciplina,con la consiguiente responsabilidad de implementar estrategias deenseñanza y evaluación que permitan identificar el grado de logro de lascompetencias profesionales a las que aspiran desarrollar en los estudiantes.El presente ensayo aborda la manera en que la Informática, como cienciaque estudia los métodos, los procesos y las técnicas de almacenamiento,procesamiento y transmisión de información, puede sentar las bases queacompañan estos cambios e introducir prácticas innovadoras tendientes aapropiarse de las virtudes del conectivismo en función del fortalecimientode competencias que hacen al perfil profesional. Según Bartolomé (2011),en el Conectivismo, como teoría del aprendizaje inicialmente propuesta porSiemens y Downes, el aprendizaje se produce a través de un proceso deconectar y generar información en el contexto de una comunidad deaprendizaje, donde esta comunidad actúa como un nodo, parte de una redmás amplia con otros nodos que comparten entre sí recursos. El tamaño yfuerza de los nodos varían de acuerdo con la concentración de información yel número de individuos participando del nodo. El conocimiento esdistribuido en la red. Algunos conceptos interesantes son el continuo cambiode la validez y precisión de la información (dinamismo del conocimiento) eigualmente el cambio en las habilidades del estudiante a lo largo del tiempo.A partir de la información recabada en el estudio, se estima que lametodología de aprendizaje auto-determinado, acompañado por los recursosque proporciona el conectivismo, crea un escenario beneficioso para la formación profesional que persigue el desarrollo de las competenciasdemandadas por la sociedad.","palabras_clave":"Conestivismo|Aprendizaje auto-determinado|Tecnologías educativas|Proceso de enseñanza aprendizaje"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4179,"idioma_id":4,"titulo":"Listado de Síntomas Breve (LSB-50) -Short Checklist of Symptoms- in Argentinean adults: Psychometric study of its main clinical scales","resumen":"The main objective of this research was studying the psychometric properties of the Listado de Síntomas Breve (LSB-50) -Short Checklist of Symptoms- in a sample of 994 Argentinean adults (49.9% females; 50.1% males). Mean age was 40.66 (SD= 17.01; Min= 18; Max= 89). This screening test has seven main clinical scales: Hypersensitivity, Obsessive-Compulsive, Anxiety, Hostility, Somatization, Depression, and Sleep disturbance. Pearson correlations indicated that all scales had positive and mostly moderate associations. The second order confirmatory factor analysis showed a good fit for a hierarchical model where all scales loaded in one major factor. Internal consistency by Cronbach?s alpha was adequate. Females scored significantly higher than males in all scales except for the Hostility scale, in which no differences were found. Although statistically significant associations with age were found in some scales, correlations were very weak. Obsessive-Compulsive, Sleep disturbance and Depression scales had the highest scores, while Anxiety presented the lowest score. Based on the psychometric evidence found, the scale seems to be suitable for the local population. Consequently, the availability of such measure may contribute to conduct epidemiological studies of psychopathology in Argentina. Moreover, the scale could be used for the assessment of psychotherapy progress and outcomes of clients, as well as for psychotherapy research. Notwithstanding, more evidence of validity and reliability should be sought.","palabras_clave":"psychological symptoms |mental health|psychopathology|adults|LSB-50"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4180,"idioma_id":5,"titulo":"Identidades juveniles en escenarios de periferización urbana. Una aproximación biográfica","resumen":"En esta comunicación analizamos en clave identitaria una serie de narrativas de jóvenescordobeses de sectores populares construidas a partir de un enfoque biográfico. Nuestroanálisis abona ciertas proposiciones que desde otras investigaciones señalan al barrio comooperador de identidad y cohesión y a la calle como lugar peligroso. Sin embargo, observamostambién que para los jóvenes la definición de su lugar de residencia como ?barrio? o?villa? supone una activa disputa simbólica, con lo cual los procesos de estigmatización noserían exclusivos del ?afuera barrial?. A lo largo del trabajo damos cuenta de algunas operacionesdiscursivas, morales y emocionales mediante las cuales los jóvenes consiguen presentarsecomo integrantes de barrios ?respetables?, marcando una distancia identitaria conciertas alteridades amenazantes que pueblan el mundo de ?la calle?: espacio físico y simbólicoque parecería constituir un desafío para la proyección juvenil hacia ciertos ?submundos?institucionales como el trabajo y la escuela.","palabras_clave":"Identidades juveniles |Sectores populares|Periferización urbana|Estigmatización"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4181,"idioma_id":4,"titulo":"Influence of second-phase inclusions on the electro-deformation of ferroelectric ceramics","resumen":"Theoretical estimates are given for the overall dissipative response of ferroelectric ceramics with second-phase inclusions, under arbitrary electromechanical loading histories. The ferroelectric behavior of the constituent phases is described via a stored energy density and a dissipation potential in accordance with the theory of generalized standard materials. An implicit time-discretization scheme is used to generate a variational representation of the overall response in terms of a single incremental potential. Estimates are then generated by constructing sequentially laminated microgeometries of particulate type whose overall incremental potential can be computed exactly. Because they are realizable, by construction, these estimates are guaranteed to conform with any material constraints, to satisfy all pertinent bounds, and to exhibit the required convexity properties with no duality gap. By way of example, the theory is used to study the influence of metallic particles and of microcavities on the electro-deformability of a lead zirconate titanate. In particular, the role of remanent polarization fluctuations on the piezoelectric properties is assessed.","palabras_clave":"COMPOSITES|POROSITY|FERROELECTRICITY|HOMOGENIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4182,"idioma_id":4,"titulo":"Association with amino acids does not enhance efficacy of polymerized liposomes as a system for lung gene delivery","resumen":"Development of improved drug and gene delivery systems directly into the lungs ishighly desirable given the important burden of respiratory diseases. We aimed toevaluate the safety and efficacy of liposomes composed of photopolymerized lipids[1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with aminoacids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles,in particular, are more stable than other types of liposomes. In this study, lipopolymerswere associated with L-arginine, L-tryptophan, or L-cysteine. We hypothesized that theaddition of these amino acids would enhance the efficacy of gene delivery to the lungs bythe lipopolymers. L-Arginine showed the highest association efficiency due to its positivecharge and better surface interactions. None of the formulations caused inflammation oraltered lung mechanics, suggesting that these lipopolymers can be safely administeredas aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue,but the addition of amino acids reduced delivery efficacy when compared with the simplelipopolymer particle. These results indicate that this system could be further explored forgene or drug delivery targeting lung diseases.Keywords: lung, gene delivery, mechanic parameters, nanoparticles, inflammation","palabras_clave":"LUNG|GENE DELIVERY|MECHANIC PARAMETERS|NANOPARTICLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4183,"idioma_id":4,"titulo":"DSPC liposomes improve transport of L-cysteine and reduce metabolic activity","resumen":"Aims: In this work, we developed and characterized liposomal formulations that encapsulate Lcysteine to study their further application in drug delivery and amino acid supplementation. The lipids used were 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC).Methodology: Encapsulation efficiency and amino acid release were determined. For biophysical characterization of the three formulations, the size, surface charge and surface packing were also studied. Cell viability was analyzed with MTT reagent after treatments with formulations ir order to study efficiency of these systems in induce changes in metabolism.Results: Results showed that L-cysteine interacts at the polar head level and that this interaction stabilizes the surface charge and prevents aggregation. We also determined the influence on cell metabolism in all formulations. The presence of L-cysteine in the DSPC formulation induced deeper changes in metabolism, evidencing that this formulation provides better transport of this amino acid.Conclusion: Liposomes developed herein are well suited for the application in the delivery of L-cysteine. Particularly, they can encapsulate nearly all the L-cysteine and can retain it for 6 hours.Also, L-cysteine stabilized liposomes, preventing their aggregation. L-cysteine encapsulated in the DSPC formulation induced deeper changes in cell metabolism, causing a decrease in metabolic activity; this was probably due to a higher entry, thus a better liposome-mediated transport. Considering that the smaller the particle, the better the circulation, we believe that the stabilization of the vesicle by L-cysteine may allow these transporters to have higher circulation times. Based on the above, we conclude that the DSPC formulation is the best suited for further application in L-cysteine delivery.Keywords: Liposomes; L-cysteine; FTIR; cell metabolism; biophysics.","palabras_clave":"Liposomes|L-cysteine|FTIR|cell metabolism|biophysics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4184,"idioma_id":4,"titulo":"Phthalocyanines as Molecular Scaffolds to Block Disease-Associated Protein Aggregation","resumen":"The aggregation of proteins into toxic conformations plays a critical role in the development of different neurodegenerative diseases such as Alzheimer?s disease (AD), Parkinson?s disease (PD), and Creutzfled?Jakob?s disease (CJD). These disorders share a common pathological mechanism that involves the formation of aggregated protein species including toxic oligomers and amyloid fibrils. The aggregation of alpha-synuclein (&#945;S) in PD and the amyloid beta peptide (A&#946;) and tau protein in AD results in neuronal death and disease onset. In the case of CJD, the misfolding of the physiological prion protein (PrP) induces a chain reaction that results in accumulation of particles that elicit brain damage. Currently, there is no preventive therapy for these diseases and the available therapeutic approaches are based on the treatment of the symptoms rather than the underlying causes of the disease. Accordingly, the aggregation pathway of these proteins represents a useful target for therapeutic intervention. Therefore, understanding the mechanism of amyloid formation and its inhibition is of high clinical importance. The design of small molecules that efficiently inhibit the aggregation process and\/or neutralize its associated toxicity constitutes a promising tool for the development of therapeutic strategies against these disorders. In this accounts, we discuss current knowledge on the anti-amyloid activity of phthalocyanines and their potential use as drug candidates in neurodegeneration. These tetrapyrrolic compounds modulate the amyloid assembly of &#945;S, tau, A&#946;, and the PrP in vitro, and protect cells from the toxic effects of amyloid aggregates. In addition, in scrapie-infected mice, these compounds showed important prophylactic antiscrapie properties. The structural basis for the inhibitory effect of phthalocyanines on amyloid filament assembly relies on specific &#960;?&#960; interactions between the aromatic ring system of these molecules and aromatic residues in the amyloidogenic proteins. Analysis of the structure?activity relationship in phthalocyanines revealed that their anti-amyloid activity is highly dependent on the type of metal ion coordinated to the tetrapyrrolic system but is not sensitive to the number of peripheral charged substituents. The tendency of phthalocyanines to oligomerize (self-association) via aromatic?aromatic stacking interactions correlates precisely with their binding capabilities to target proteins and, more importantly, determines their efficiency as anti-amyloid agents. The ability to block different types of disease-associated protein aggregation raises the possibility that these cyclic tetrapyrrole compounds have a common mechanism of action to impair the formation of a variety of pathological aggregates. Because the structural and molecular basis for the anti-amyloid effects of these molecules is starting to emerge, combined efforts from the fields of structural, cellular, and animal biology will result critical for the rational design and discovery of new drugs for the treatment of amyloid related neurological disorders.","palabras_clave":"phthalocyanines|neurodegenerative|Aggregation|Parkinson"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4185,"idioma_id":4,"titulo":"A high-altitude peatland record of environmental changes in the NW Argentine Andes (24\u000E S) over the last 2100 years","resumen":"High-altitude cushion peatlands are versatile archives for high-resolution palaeoenvironmental  studies, due to their high accumulation rates, range of proxies and sensitivity  to climatic and\/or human-induced changes. Especially within the central Andes,  5 the knowledge about climate conditions during the Holocene is limited. In this study,  we present the environmental and climatic history for the last 2100 years of Cerro Tuzgle  peatland (CTP), which is located in the dry Puna of NW Argentina, based on a  multi-proxy approach. X-ray fluorescence (XRF), stable isotope and element content  analyses (&#948;  13C, &#948;  15N, TN and TOC) were conducted to analyse the inorganic geo-  10 chemistry throughout the sequence, revealing changes in the peatland?s past redox  conditions. Pollen assemblages give an insight into substantial environmental changes  on a regional scale. The palaeoclimate varied significantly during the last 2100 years.  The results reflect prominent late Holocene climate anomalies and provide evidence  that Northern Hemisphere temperature oscillations were extensive and affected the  15 southward migration of the Intertropical Convergence Zone (ITCZ), and hence, the  intensity of moisture flux within the South American Summer Monsoon (SASM) belt.  Volcanic forcing at the beginning of the 19th century (1815 Tambora eruption) seems to  have had an impact on climatic settings in the central Andes. In the past, the peatland  recovered from climatic perturbations. Nowadays, CTP is heavily degraded by human  20 interventions, and the peat deposit becomes increasingly susceptible to erosion and  incision.","palabras_clave":"HIGH-ANDEAN VEGETATION|HIGH-RESOLUTION PALAEOENVIRONMENTAL STUDIES|LATE HOLOCENE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4186,"idioma_id":5,"titulo":"Posicionamientos ideológicos de ciudadanos argentinos que se ubican en el 'centro' o ?no poseen ideología?: aportes desde la Psicología Política","resumen":"Este trabajo intenta aportar a la comprensión de los posicionamientos ideológicos de ciudadanosde Córdoba (Argentina) que no se auto-ubican en el espectro ideológico o que se posicionanen el ?centro?. Se efectuó un estudio cuantitativo con dos muestras de ambos sexos:estudiantes universitarios y población general. Se aplicó un cuestionario cerrado queindagaba variables socio-demográficas, auto-posicionamiento ideológico y posicionamientosideológicos ante tópicos situacionales (seguridad, drogas, aborto, economía, democracia,entre otros). Se efectuaron Análisis de Varianza para conocer las diferencias entre los gruposen cuanto a sus posicionamientos. Los resultados indican perfiles diferenciales entre losgrupos, y ratifican que estas personas detentan posiciones ideológicas conservadoras y progresistasaunque simbólicamente no se identifiquen con la izquierda ni con la derecha. Enfunción de los resultados, se discute la utilidad de trascender el análisis de la ideología políticacentrado exclusivamente en su dimensión simbólica, avanzando hacia mediciones operativasde la ideología política.","palabras_clave":"IDEOLOGÍA POLÍTICA|AUTO-POSICIONAMIENTO IDEOLÓGICO|CENTRO|POSICIONAMIENTOS IDEOLÓGICOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4187,"idioma_id":4,"titulo":"A basal Lithostrotian Titanosaur (Dinosauria: Sauropoda) with a complete skull: Implications for the evolution and paleobiology of Titanosauria","resumen":"We describe Sarmientosaurus musacchioi gen. et sp. nov., a titanosaurian sauropod dinosaurfrom the Upper Cretaceous (CenomanianTuronian) Lower Member of the Bajo BarrealFormation of southern Chubut Province in central Patagonia, Argentina. The holotypicand only known specimen consists of an articulated, virtually complete skull and part of thecranial and middle cervical series. Sarmientosaurus exhibits the following distinctive featuresthat we interpret as autapomorphies: (1) maximum diameter of orbit nearly 40% rostrocaudallength of cranium; (2) complex maxillalacrimal articulation, in which the lacrimalclasps the ascending ramus of the maxilla; (3) medial edge of caudal sector of maxillaryascending ramus bordering bony nasal aperture with low but distinct ridge; (4) tongue-likeventral process of quadratojugal that overlaps quadrate caudally; (5) separate foramina forall three branches of the trigeminal nerve; (6) absence of median venous canal connectinginfundibular region to ventral part of brainstem; (7) subvertical premaxillary, procumbentmaxillary, and recumbent dentary teeth; (8) cervical vertebrae with strut-like centroprezygapophyseallaminae; (9) extremely elongate and slender ossified tendon positioned ventrolateralto cervical vertebrae and ribs. The cranial endocast of Sarmientosaurus preservessome of the most complete information obtained to date regarding the brain and sensorysystems of sauropods. Phylogenetic analysis recovers the new taxon as a basal member ofLithostrotia, as the most plesiomorphic titanosaurian to be preserved with a complete skull.Sarmientosaurus provides a wealth of new cranial evidence that reaffirms the close relationshipof titanosaurs to Brachiosauridae. Moreover, the presence of the relatively derivedlithostrotian Tapuiasaurus in Aptian deposits indicates that the new Patagonian genus representsa ghost lineage with a comparatively plesiomorphic craniodental form, the evolutionaryhistory of which is missing for at least 13 million years of the Cretaceous. The skullanatomy of Sarmientosaurus suggests that multiple titanosaurian species with dissimilarcranial structures coexisted in the early Late Cretaceous of southern South America. Furthermore,the new taxon possesses a number of distinctive morphologiessuch as theossified cervical tendon, extremely pneumatized cervical vertebrae, and a habitually downward-facing snoutthat have rarely, if ever, been documented in other titanosaurs, thusbroadening our understanding of the anatomical diversity of this remarkable sauropodclade. The latter two features were convergently acquired by at least one penecontemporaneousdiplodocoid, and may represent mutual specializations for consuming low-growingvegetation.","palabras_clave":"Skull|Lithostrotian titanosaur|Evolution|Paleobiology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4188,"idioma_id":4,"titulo":"Bovine serum albumin binding, antioxidant and anticancer properties of an oxidovanadium(IV) complex with luteolin","resumen":"Chemotherapy using metal coordination compounds for cancer treatment is the work of the ongoing research. Continuing our research on the improvement of the anticancer activity of natural flavonoids by metal complexation, a coordination compound of the natural antioxidant flavone luteolin (lut) and the oxidovanadium(IV) cationhas been synthesized and characterized. Using different physicochemical measurements some structuralaspects of [VO(lut)(H2O)2]Na·3H2O (VOlut) were determined. The metal coordinated to two cis-deprotonated oxygen atoms (ArO&#8722;) of the ligand and two H2O molecules. Magnetic measurements in solid state indicated the presence of an effective exchange pathway between adjacent vanadiumions. VOlut improved the antioxidant capacity of luteolin only against hydroxyl radical. The antitumoral effects were evaluated on MDAMB231 breast cancer and A549 lung cancer cell lines. VOlut exhibited higher viability inhibition (IC50=17 &#956;M) than the ligandon MDAMB231 cells but they have the same behavior on A549 cells (ca. IC50=60 &#956;M). At least oxidative stress processes were active during cancer cell-killing. When metals chelated through the carbonyl group and one adjacent OH group of the flavonoid an effective improvement of the biological properties has been observed. InVOlut the different coordination may be the cause of the small improvement of some of the tested properties of the flavonoid. Luteolin and VOlut could be distributed and transported in vivo. Luteolin interacted in the microenvironmentof the tryptophan group of the serum binding protein, BSA, by means of electrostatic forces and its complex bind the protein by H bonding and van derWaals interactions.","palabras_clave":"Luteolin|Oxidovanadium(IV) complexes|Magnetic properties|Mechanisms of cytotoxic action|BSA binding"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4189,"idioma_id":4,"titulo":"Latin American Lidar Network (LALINET): a diagnostic on networking instrumentation","resumen":"LALINET (Latin American Lidar Network), previously knownas ALINE, is the first fully operative lidar Network for aerosol research in SouthAmerica, probing the atmosphere on regular basis since September 2013. The generalpurpose of this network is to attempt to fill the gap in the knowledge on aerosolvertical distribution over South America and its direct and indirect impact on weatherand climate by the establishment of a vertically-resolved data set of aerosol properties.Similarly to other lidar research networks, most of the LALINET instruments arenot commercially produced and, consequently, configurations, capabilities and derived-productscan be remarkably different among stations. It is a fact that such un-biased 4Ddata set calls for a strict standardization from the instrument a land data processingpoint of view. This study has been envisaged to investigate the ongoing networkconfigurations with the aim of highlighting the instrumental strengths and weaknessesof LALINET.","palabras_clave":"AEROSOL DETECTION| LIDAR|NETWORK|REMOTE SENSING AND SENSORS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4190,"idioma_id":4,"titulo":"Improvement of Bovine Semen Quality by Removal of Membrane-Damaged Sperm Cells with DNA Aptamers and Magnetic Nanoparticles","resumen":"In cattle, cryopreservation of semen and sex-sorting kill up to 50% of spermatozoa and decrease the success of assisted insemination (AI). Therefore, significant efforts are being carried out to improve the quality of semen prior to AI. In this work we used the Cell-SELEX technique to select single strand DNA aptamers able to recognize with high affinity and specificity damaged sperm cells generated by heat-treatment. We first isolated aptamers with a conserved two motifs of 6 nucleotides of length that bind to the membrane of heat-treated spermatozoa. Then, we used synthetic biotin-labeled aptamers containing the conserved motif to recognize membrane-damaged cells and separate them from viable cells by the use of avidin-coated superparamagnetic iron oxide nanoparticles (SPION). This procedure improved the quality of semen by significantly increasing the percentage of healthy sperm cells without affecting the rate of blastocyst cleavage. This technique was successfully applied to both unsorted and sex-sorted sperm suspension.","palabras_clave":"DNA APTAMERS|MAGNETIC NANOPARTICLES|SEMEN QUALITY|BOVINE|CELL-SELEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4191,"idioma_id":4,"titulo":"DETERMINING THE GEOGRAPHICAL ORIGIN OF Sechium edule FRUITS BY MULTIELEMENT ANALYSIS AND ADVANCED CHEMOMETRIC TECHNIQUES","resumen":"This paper describes the determination and evaluation of the major and trace element composition (Al, As, Ba, Ca, Cd, Co, Cu, Fe, K, Mg, Mn, Na, Pb, Sr and Zn) of Sechium edule (Jacq) Swartz fruits collected from four different places of production in Corrientes province, Argentina. Element concentrations were determined by using inductively coupled plasma optical emission spectrometry (ICP OES) after microwave digestion. The accuracy was confirmed with standard reference material of spinach leaves (NIST, 1570a) and spiking tests. Principal component analysis (PCA), linear discriminant analysis (LDA), k-nearest neighbors (kNN), partial least square-discriminant analysis (PLS-DA) and support vector machine (SVM) were applied to the results for discriminating the geographical origin of S. edule fruits. Finally, the LDA method was found to perform best with up to 90% accuracy rate based on the following elements: Ca, Ba, Cu, Mn, Na, Sr, and Zn.","palabras_clave":"ICP OES|MINERAL COMPOSITION|MULTIVARIATE ANALYSIS|CLASSIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4192,"idioma_id":4,"titulo":"Synthesis, physicochemical and biological studies of a ternary Co(II) complex with sulfaquinoxaline and 2,2 0 -bipyrimidine as ligands","resumen":"A novel ternary complex of Cobalt(II) with 4-amino-N-2-quinoxalinylbenzenesulfonamide (sulfaquinoxaline,SQO), and 2,20-bipyrimidine (Bpym) as ligands has been prepared. The complex has been characterizedbased on elemental analyses, FTIR and Raman spectroscopy. Its structure, [Co(SQO)2(Bpym)] wasdetermined by X-ray diffraction methods. It crystallizes in the triclinic P1 space group with a = 10.5381(2), b = 13.6469(2), c = 13.8409(3) Å, a = 95.058(2), b = 93.769(2), c = 93.750(2) and Z = 2 moleculesper unit cell. Thermogravimetric (TG) and differential thermal analysis (DT) were also studied.The aquatic toxicity of the complex was evaluated on the two following test organisms: Danio rerio(cebritas) y Cyprinus carpio haematopterus (carpas koi). Antibacterial activity was screened againstEscherichia coli (ATCC 8739), Salmonella typhimurium (ATCC 14028), Staphylococcus aureus (ATCC 49775)and Bacillus cereus (ATCC 10987). The antiproliferative effect of the tested complex [Co(SQO)2(Bpym)] inthe human osteoblast (MG-63) cell line was also studied.","palabras_clave":"Sulfaquinoxaline |2,2-Bipyrimidine|Spectroscopic properties| Co(II) complex"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4193,"idioma_id":4,"titulo":"Malnutrition and Body Composition in Urban and Rural Schoolchildren: A Cross-sectional Study in San Rafael, Mendoza (Argentina)","resumen":"Objectives: The aim of the present study was to analyze the nutritional status and body composition of childrenfrom San Rafael, Mendoza, avoiding urban and rural categorization by generating subpopulations as a function of theirsocio-environmental characteristics.Methods: A cross-sectional study was performed in 3,596 schoolchildren from 4.0 to 13.9 years of age. Body weight,height, upper arm circumference, and triceps skinfold were measured. Body mass index, total muscle, and fat areas ofthe arm were calculated. To estimate nutritional status and body composition, the NHANES III reference was used.The socio-environmental variables were surveyed using a structured questionnaire. These variables were processed bycategorical principal-component analysis (catPCA).Results: The catPCA allowed the differentiation of four groups, three with urban characteristics (high urban,medium urban, impoverished urban), and a rural group. Stunting occurred at a higher rate in the impoverished urbangroup, and the occurrence of underweight children was higher in the rural group. The prevalence of excess weight variedin the range of 20?26%. The latter value corresponded to children of the high urban group, who also did not showreduced muscle mass.Conclusion: Children from San Rafael presented differences in nutritional condition and body composition associatedwith pronounced socio-environmental heterogeneity. Thus, we could observe a gradient from the ?high urban? group, withbetter social, economic, and sanitation conditions and at the same time a more obesogenic environment to the?impoverished urban? and ?rural? groups, whose vulnerability reflected a higher prevalence of child undernutrition and theassociation with excess weight and reduced muscle mass.","palabras_clave":"MALNUTRITION|BODY COMPOSITION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4194,"idioma_id":4,"titulo":". Effects of ethanol exposure in a familiar or isolated context during infancy on ethanol intake during adolescence.","resumen":"Early exposure to ethanol affects ethanol intake later in life. This early experience encompasses exposure to social stimuli and the pharmacological and orosensory properties of ethanol. The specific contribution of each type of stimulus to subsequent ethanol intake remains unknown. We assessed the intake of various concentrations of ethanol in a familiar or isolated context during infancy and the lingering effects of this experience on ethanol intake during adolescence. On postnatal day 3 (PD3), PD7, and PD11, rats were given 5% ethanol or water in a nursing or isolated context (Experiments 1 and 2). Intake tests (ethanol vs. water) were conducted during adolescence. Experiment 2 matched the amount of fluid ingested during infancy in both contexts and subsequently tested ethanol consumption during adolescence. The results revealed a facilitative effect of the nursing context on fluid intake during the tests in infancy. Pups stimulated with ethanol but not water in the isolated context exhibited an increase in ethanol consumption during adolescence. This effect disappeared when the isolated infants were matched to receive the same amount of ethanol ingested by their nursed counterparts. In Experiment 3, isolated infant rats were exposed to different ethanol concentrations (0.0%, 2.5%, 5.0%, and 10.0%), and drug consumption was tested during adolescence. This exposure increased adolescent ethanol intake, regardless of the alcohol concentration (Experiment 3). The common denominators that resulted in enhanced ethanol intake during adolescence were preexposure to ethanol via active consumption of the drug that induced a low-to-moderate level of intoxication in an isolated context.","palabras_clave":"adolescent|ethanol|home enviroment|infant"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4195,"idioma_id":4,"titulo":"Physical quality indicators and mechanical behavior of agricultural soils of Argentina","resumen":"Mollisols of Santa Fe have differenttilth and load support capacity. Despite the importance of these attributes toachieve a sustainable crop production, few information is available. Theobjectives of this study are i) to assess soil physical indicators related toplant growth and to soil mechanical behavior; and ii) to establishrelationships to estimate the impact of soil loading on the soil quality toplant growth. The study was carried out on Argiudolls and Hapludolls of SantaFe. Soil samples were collected to determine texture, organic matter content,bulk density, water retention curve, soil resistance to penetration, leastlimiting water range, critical bulk density for plant growth, compressionindex, pre-consolidation pressure and soil compressibility. Water retentioncurve and soil resistance to penetration were linearly and significantlyrelated to clay and organic matter (R2 = 0.91 and R2 = 0.84). The pedotransfer functions of water retentioncurve and soil resistance to penetration allowed the estimation of the leastlimiting water range and critical bulk density for plant growth. A significantnonlinear relationship was found between critical bulk density for plant growthand clay content (R2 = 0.98).Compression index was significantly related to bulk density, water content,organic matter and clay plus silt content (R2 = 0.77). Pre-consolidation pressure wassignificantly related to organic matter, clay and water content (R2 = 0.77). Soilcompressibility was significantly related to initial soil bulk density, clayand water content. A nonlinear and significantly pedotransfer function (R2 = 0.88) wasdeveloped to predict the maximum acceptable pressure to be applied duringtillage operations by introducing critical bulk density for plant growth in thecompression model. The developed pedotransfer function provides a useful toolto link the mechanical behavior and tilth of the soils studied. ","palabras_clave":"soil quality|pedotransfer functions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4196,"idioma_id":4,"titulo":"Managing the value of composts as organic amendments and fertilizers in sandy soils","resumen":"Nutrient rich composts are employed at low rates to minimize risks of N and P losses; this limits their value as soil improvers through C addition and the build up of soil organic matter. Blending with nutrient-poor composts such as those from the organic fraction of municipal solid waste could reduce the risks of nutrient losses while maintaining the positive effects on soil organic matter. We conducted a 2-yr experiment with composts of diverse origin: organic fraction of municipal solid waste (MC), cattle feedlot manure (FC), poultry litter (PC) and biosolids (BC), alone or blended (FC-MC, PC-MC) in a sandy soil under the humid warm climatic conditions of NE Argentina. We studied the effects of a single application (40 Mg ha&#8722;1) on the surface soil (0?10 cm) properties of a permanent subtropical pasture through annual chemical and biological analyses. On five dates, available N and P were also determined at 0?10 cm and 55?65 cm. Soil total C and N increased over time while potential N mineralization and CO2 emission decreased. All amendments resulted in similar increments of soil C and N despite marked differences in quantity and quality of organic matter inputs. Because MC had substantial amounts of Ca carbonates, it contributed to a reduction of available P from manure composts through dilution and precipitation. The release of available P from biosolids composts (where P is bound to Fe and Al) was lower than from manure composts (where P is bound by Ca phosphates). The highest environmental risk from compost application would likely be the leaching of soluble N produced during the composting process and released immediately after field application. Blending with N-poor MC would contribute to nitrate dilution.","palabras_clave":"Biosolids|Animal manures|Municipal solid waste|Compost mixtures|Organic matter|Nutrients|Ca carbonates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4197,"idioma_id":4,"titulo":"The sparkling Universe: the coherent motions of cosmic voids","resumen":"We compute the bulk motions of cosmic voids, using a &#923; cold dark matter numerical simulation considering the mean velocities of the dark matter inside the void itself and that of the haloes in the surrounding shell. We find coincident values of these two measures in the range ?300-400 km s-1, not far from the expected mean peculiar velocities of groups and galaxy clusters. When analysing the distribution of the pairwise relative velocities of voids, we find a remarkable bimodal behaviour consistent with an excess of both systematically approaching and receding voids. We determine that the origin of this bimodality resides in the void large-scale environment, since once voids are classified into void-in-void (R-type) or void-in-cloud (S-type), R-types are found mutually receding away, while S-types approach each other. The magnitude of these systematic relative velocities account for more than 100 km s-1, reaching large coherence lengths of up to 200 h-1 Mpc . We have used samples of voids from the Sloan Digital Sky Survey Data Release 7 and the peculiar velocity field inferred from linear theory, finding fully consistent results with the simulation predictions. Thus, their relative motion suggests a scenario of a sparkling universe, with approaching and receding voids according to their local environment.","palabras_clave":"COSMOLOGY: OBSERVATIONS|COSMOLOGY: LARGE-SCALE STRUCTURE OF THE UNIVERSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4198,"idioma_id":4,"titulo":"YUPI, a regional footprint calculator","resumen":"AbstractPurposeMany tools to quantify the environmental impact of human decisions have been developed, but all of them seem to have a limited application at the regional or local level. A free-of-charge, Argentina-based personal footprint calculator software (YUPI®) has been developed in order to raise awareness among local citizens about the environmental impacts generated by their daily habits. The extensive use of the tool will generate information suitable for future scientific studies based on local data.MethodsThe software calculates the ecological, carbon, and water footprints of individuals, implementing specific regional data from Argentina developed by the CLIOPE group, complemented with data from the Water Footprint Network and the Global Footprint Network. The calculator was developed focusing on interface attractiveness, ease of use, language simplicity, and a good trade-off between completion time and fullness.Results and discussionThe YUPI® software allows its users to understand at a glance their contribution to the environmental impacts of modern society and to quantify the reduction opportunities they have at hand. The program?s language and variables reflect local lifestyle choices, making the filling process accessible for children. The calculator was placed online as an educational tool for teachers and students from all educational levels, and it was also used by visitors in local science and educational fairs. Valuable data was collected for future initiatives on impact mitigation.ConclusionsAmplified by the mass media, the new tool has helped raise awareness and discussion about the individual environmental footprint, both in the educational and in the domestic terrain. The strategy of creating a simple, easily administered, and widely available quiz helped bridge the gap between the academy and the people, making available to them the continuously updated information generated by the research groups. This is facilitating citizen not only to understand the complexity of the environmental problems but also to take informed actions leading to their mitigation.","palabras_clave":"ARGENTINA|CARBON FOOTPRINT|COMMUNITY OUTREACH  |ECOLOGICAL FOOTPRINT|ONLINE CALCULATOR|WATER FOOTPRINT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4199,"idioma_id":4,"titulo":"Effect of different combinations of glycerol and\/or trehalose on physical and structural properties of whey protein concentrate-based edible films","resumen":"The aim of the present study was to develop and characterize edible films produced from whey protein concentrate (WPC) and plasticized with different contents of glycerol (Gly) and\/or trehalose (Tre) in order to evaluate new edible film formulations for their potential use in food packaging applications.Additionally, potential changes in the film mechanical properties during storage at ambient and freezing conditions were considered. Moisture content, solubility, thickness, transparency, microstructure, colour parameters, and mechanical properties were assessed. The films incorporated with Tre were moreinsoluble in water than WPC\/Gly films, being more suitable for food  Applications. WPC\/Gly and WPC\/Tre films were clear enough to be used as see-through packaging. However, when Tre was included into WPC\/Gly film  Formulations, film opacity increased. Scanning electron microscope (SEM) images suggested that this phenomenon may be related to the growth of Tre crystals in the film matrix. Moreover, when Tre concentration increased in the WPC\/Gly matrix, film surface was more heterogeneous. Interestingly, the presence of Tre in WPC-based films was effective in preventing Maillard reaction after heating. WPC\/Tre films were the most rigid but the least stable for storage, resulting more susceptible to rupture and cracking. Only WPC\/Gly and WPC\/Gly-Tre 8% films were rather flexible, manageable, and stable up to 90 days of storage under ambient and freezing conditions. These findings can be used to better design applications of edible films containing plasticizers that may crystallize over time in order to optimize film formulation in a rational manner towards their eventual application as food packaging.","palabras_clave":"WHEY PROTEIN EDIBLE FILMS|TREHALOSE|PHYSICAL PROPERTIES|LONG-TERM STORAGE|FREEZING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4200,"idioma_id":4,"titulo":"Antimicrobial and physicochemical characterization of whey protein concentrate edible films incorporated with liquid smoke","resumen":"The incorporation of commercial liquid smoke (LS) to edible films was investigated for the first time. The objective of this investigation was to characterize whey protein concentrate (WPC)-based edible films incorporated with LS. According to the bactericidal activity of LS against Escherichia coli, Staphylococcus aureus, Salmonella Typhimurium, and Listeria monocytogenes in liquid medium, WPC-based films incorporated with 0, 5, 10, and 15% (v\/v) LS were prepared. Inhibition zone in solid media, thickness, transparency, color and mechanical properties of the films were analyzed. Films including LS wereeffective to prevent growth of L. monocytogenes in the agar diffusion test. Analyzing color parameters, the incorporation of LS into films caused a decrease in L* and an increase in both a* and b*. However, these sensory changes were not detrimental for their potential use in food applications. Noticeably, tensilestrength and elongation tend to increase when LS was added into films  Formulation. Depending on its content, different protein-LS interactions could be generated, positively affecting the mechanical properties of films. In conclusion, WPC-based edible films incorporated with LS may be suitable for being applied to food surfaces and useful to prevent the superficial growth of the globally recognized high-risk foodborne pathogen Listeria monocytogenes.","palabras_clave":"WHEY PROTEIN FILMS|LIQUID SMOKE|ANTIMICROBIAL PROPERTIES|PHYSICOCHEMICAL CHARACTERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4201,"idioma_id":4,"titulo":"Coadministration of cruzipain and GM-CSF DNAs, a new immunotherapeutic vaccine against Trypanosoma cruzi infection","resumen":"Therapeutic vaccine research and development are especially important in Chagas diseaseconsidering the characteristics of the chronic infection and the number of people in theAmericas living with a parasite infection for decades. We have previously reported the efficacyof attenuated Salmonella enterica (S) carrying plasmid encoding cruzipain (SCz) to protectagainst Trypanosoma cruzi infection. In the present work we investigated whether Cz DNAvaccine immunotherapy could be effective in controlling an ongoing T. cruzi infection in mice.We here report the intramuscular administration of naked Cz DNA or the oral administration ofSalmonella as Cz DNA delivery system as therapeutic vaccines in mice during acute or chronicinfection. The coadministration of a plasmid encoding GM-CSF improved vaccine performance,indicating that the stimulation of innate immune cells is needed in the event of an ongoinginfection. These therapeutic vaccines were able to address the response to a protective andsustained Th1 biased profile not only against Cz but also against a variety of parasite antigens.The combined therapeutic vaccine during the chronic phase of infection prevents tissuepathology as shown by a reduced level of enzyme activity characteristic of tissue damage and atissue status compatible with normal tissue. The obtained results suggest that immunotherapywith Cz and GM-CSF DNAs, either alone or in combination with other drug treatments, mayrepresent a promising alternative for Chagas disease therapy.","palabras_clave":"Trypanosoma cruzi|Chagas disease|Therapeutic DNA vaccine|Salmonella enterica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4202,"idioma_id":4,"titulo":"The Major Soybean Allergen Gly m Bd 28K Induces Hypersensitivity Reactions in Mice Sensitized to Cow's Milk Proteins","resumen":"Reactions to soy have been reported in a proportion of patients with IgE-mediated cow's milk allergy (CMA). In this work, we analyzed if Gly m Bd 28K \/P28, one of the major soybean allergens, is a cross-reactive allergen with cow milk proteins (CMP). We showed that P28 was recognized by IgE sera from CMA patients and activated human peripheral basophils degranulation. Moreover, IgE sera of mice exclusively sensitized to CMP recognized P28. Splenocytes from sensitized animals secreted IL-5 and IL-13 when incubated with CMP or soy proteins, but only IL-13 when treated with P28. In addition, skin test was strongly positive for CMP and weakly positive for P28. Remarkably, milk-sensitized mice showed hypersensitivity symptoms following sublingual challenge with P28 or CMP. Using bioinformatics´ tools seven putative cross-reactive epitopes were identified. In conclusion, using in vitro and in vivo test we demonstrated that P28 is a novel cross-reactive allergen with CMP.","palabras_clave":"Food allergy|P28 soybean protein|Bovine caseins| Cross-reactivity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4203,"idioma_id":4,"titulo":"miR-190 enhances HIF-dependent responses to hypoxia in Drosophila by inhibiting the prolyl-4-hydroxylase Fatiga","resumen":"Cellular and systemic responses to low oxygen levels are principally mediated by Hypoxia Inducible Factors (HIFs), a family of evolutionary conserved heterodimeric transcription factors, whose alpha- and beta-subunits belong to the bHLH-PAS family. In normoxia, HIF&#61537; is hydroxylated by specific prolyl-4-hydroxylases, targeting it for proteasomal degradation, while in hypoxia the activity of these hydroxylases decreases due to low oxygen availability, leading to HIF&#61537; accumulation and expression of HIF target genes. To identify microRNAs required for maximal HIF activity, we conducted an overexpression screen in Drosophila melanogaster, evaluating the induction of a HIF transcriptional reporter. miR-190 overexpression enhanced HIF-dependent biological responses, including terminal sprouting of the tracheal system, while in miR-190 loss of function embryos the hypoxic response was impaired. In hypoxic conditions, miR-190 expression was upregulated and required for induction of HIF target genes by directly inhibiting the HIF prolyl-4-hydroxylase Fatiga. Thus, miR-190 is a novel regulator of the hypoxia response that represses the oxygen sensor Fatiga, leading to HIF&#61537; stabilization and enhancement of hypoxic responses.","palabras_clave":"Hypoxia Inducible Factor|oxygen sensor|miRNA screen|fruit fly"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4204,"idioma_id":4,"titulo":"Reproductive isolation between two populations of Diatraea saccharalis (F.) (Lepidoptera: Crambidae) from different host plant species and regions in Argentina","resumen":"The sugarcane borer, Diatraea saccharalis (F.), has a widespread distributionthroughout the Western Hemisphere and is a pest of many crop plants including sugarcane, corn, sorghum and rice. The use of Bacillus thuringiensis (Bt) corn has been the primary tool for managing this species in corn fields. Sugarcane borer control has been recently threatened by observations of susceptibility and\/or resistance to certain varieties of Bt corn and the protein used in many newer varieties. This has led to increased interest in understanding sugarcane borer genetic diversity and gene flow within and among its populations and the consequent exchange of alleles between geographically distant populations. The objective of this study was to examine reproductive compatibility between host-associated geographic populations of D. saccharalis in Argentina and to determine whether this pest represents a complex of host-associated cryptic species rather than a wide ranging generalist species. Intra and inter-population crosses revealed that D. saccharalis populations from the northwestern and Pampas regions presented evidence of prezygotic and postzygotic incompatibility. Such a result is likely to be the product of an interruption of gene flow produced by either geographic or host plant associated isolation, suggesting that Tucumán (northwestern) and Buenos Aires (Pampas) populations of D. saccharalis are a distinct genotype and possibly an incipient species.","palabras_clave":"sugarcane borer|fitness,|reproductive compatibility|cryptic species,|insect resistance management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4205,"idioma_id":4,"titulo":"The TIP60 complex is a conserved coactivator of HIF1A","resumen":"Hypoxia inducible factors (HIFs) are critical regulators of the cellular response to hypoxia. Despite their established roles in normal physiology and numerous pathologies, the molecular mechanisms by which they control gene expression remain poorly understood. We report here a conserved role for the TIP60 complex as a novel HIF1 transcriptional cofactor in Drosophila and human cells. TIP60 (KAT5) is required for HIF1-dependent gene expression in fly cells and embryos and colorectal cancer cells. HIF1A interacts with and recruits TIP60 to chromatin. TIP60 is dispensable for HIF1A association with its target genes but is required for HIF1A-dependent chromatin modification and RNA polymerase II activation in hypoxia. In human cells, global analysis of HIF1A-dependent gene activity reveals that most HIF1A targets require either TIP60, the CDK8-Mediator complex, or both as co-activators for full expression in hypoxia. Thus, HIF1A employs functionally diverse cofactors to regulate different subsets of genes within its transcriptional program.","palabras_clave":"Hypoxia Inducible Factor|Acetyl transferase|Transcription|Histone"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4206,"idioma_id":4,"titulo":"Exploring the ontogenetic scaling hypothesis during the diversification of pollination syndromes in Caiophora (Loasaceae, subfam. Loasoideae)","resumen":"Background and aims Phenotypic diversification of flowers is frequently attributed to selection by different functional groups of pollinators.                     During optimization of floral phenotype, developmental robustness to genetic and non-genetic perturbations is expected to                     limit the phenotypic space available for future evolutionary changes. Although adaptive divergence can occur without altering                     the basic developmental programme of the flower (ontogenetic scaling hypothesis), the rarity of reversion to ancestral states                     following adaptive radiations of pollination syndromes suggests that changes in the ancestral developmental programme of the                     flower are common during such evolutionary transitions. Evidence suggests that flower diversification into different pollination                     syndromes in the Loasoideae genus Caiophora took place during a recent adaptive radiation in the central Andes. This involved transitions from bee to hummingbird and                     small rodent pollination. The aim of this work was to examine if the adaptive radiation of pollination syndromes in Caiophora occurred through ontogenetic scaling or involved a departure from the ontogenetic pattern basal to this genus.                                    Methods We used geometric morphometric variables to describe the shape and size of floral structures taking part in the pollination                     mechanism of Loasoideae. This approach was used to characterize the developmental trajectories of three species basal to the                     genus Caiophora through shape?size relationships (ontogenetic allometry). We then tested if the shape?size combinations of these structures                     in mature flowers of derived Caiophora species fall within the phenotypic space predicted by the development of basal species.                                    Key Results Variation in the size and shape of Caiophora flowers does not overlap with the pattern of ontogenetic allometry of basal species. Derived bee-, hummingbird- and rodent-pollinated                     species had divergent ontogenetic patterns of floral development from that observed for basal bee-pollinated species.                                    Conclusions The adaptive radiation of Caiophora involved significant changes in the developmental pattern of the flowers, rejecting the ontogenetic scaling hypothesis.                  ","palabras_clave":"LOASACEAE|lOASOIDEAE|CAIOPHORA|LOASA|BLUMEMBACHIA|ADAPTIVE RADIATION|POLLINATION SYNDROME|REVERSION|ONTOGENETIC ALLOMETRY|DEVELOPMENTAL ROBUSTNESS|ONTOGENETIC SCALING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4207,"idioma_id":4,"titulo":"Using chromosomal data in the phylogenetic and molecular dating framework: Karyotype evolution and diversification in Nierembergia (Solanaceae) influenced by historical changes in sea level","resumen":"Karyotype data within a phylogenetic framework and molecular dating wereused to examine chromosome evolution in Nierembergiaand to infer how geological or climatic processes have influenced in thediversification of this solanaceous genus native to South America and Mexico. Despitethe numerous studies comparing karyotype features across species, including theuse of molecular phylogenies, to date relatively few studies have used formalcomparative methods to elucidate chromosomal evolution, especially to reconstructthe whole ancestral karyotypes. Here, we mapped on the Nierembergia phylogeny one complete set of chromosomal dataobtained by conventional staining, AgNOR-, C- and fluorescent chromosomebanding, and fluorescent in situhybridization. In addition, we used a Bayesian molecular relaxed clock toestimate divergence times between species. Nierembergiashowed two major divergent clades: a mountainous species group with symmetricalkaryotypes, large chromosomes, only one nucleolar organizing region (NOR), andwithout centromeric heterochromatin, and a lowland species group withasymmetrical karyotypes, small chromosomes, two chromosomes pairs with NORs andcentromeric heterochromatin bands. Molecular dating on the DNA phylogenyrevealed that both groups diverged during Late Miocene, when Atlantic marineingressions called the ?Paranense Sea? probably forced the ancestors of thesespecies to find refuge in unflooded areas for about 2 M years. This splitagrees with an increased asymmetry and heterochromatin amount, and decrease inkaryotype length and chromosome size. Thus, when the two Nierembergia ancestral lineages were isolated, major divergencesoccurred in chromosomal evolution, and then each linage underwent speciationseparately, with relatively minor changes in chromosomal characteristics.","palabras_clave":"KARYOTYPE ANCESTRAL RECONSTRUCTION|CHROMOSOME EVOLUTION|MOLECULAR DATING|FLUORESCENT IN SITU HYBRIDIZATION|CHROMOSOME BANDING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4208,"idioma_id":4,"titulo":"Beyond neutral and forbidden links: morphological matches and the assembly of mutualistic hawkmoth-plant networks.","resumen":"no disponible","palabras_clave":"POLLINATION|NETWORK|SPHINGIDAE|MATCHING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4209,"idioma_id":4,"titulo":"Flower reshaping in the transition to hummingbird pollination in Loasaceae subfam. Loasoideae despite absence of corolla tubes or spurs","resumen":"Many angiosperm lineages present transitions from bee to hummingbird pollination. The flower design in most of these lineages includes either corolla tubes or nectar spurs, structures that commonly experienced an elongation with the acquisition of hummingbird pollination. It is proposed that this increases the fit between the bird head and flower structures, and isolates or partially blocks bees from the interaction. But can this transition occur if the ancestral flower design lacks tubes or spurs? Here we focus on the transition from bee to hummingbird pollination in the Loasaceae subfamily Loasoideae. Loasoideae flowers have radial corollas with separated petals; therefore, they do not display corolla tubes nor nectar spurs. These flowers also present a whorl of nectar scales and staminodes, unique to the subfamily, which is involved in flower?pollinator fit and in nectar harvesting. To explore flower shape adaptation to hummingbird pollination, we tested for correspondence between pollinators and flower shape in Loasoideae. In order to achieve this, we first compared the evolutionary history of flower phenotype and pollination mode, and then used stochastic character mapping and geometric-morphometric variables in a comparison of alternative evolutionary models. The results of our study suggest that the transition from bee to bird pollination was accompanied by changes in the shape of the staminodial complex, along with the evolution of relatively closed corollas.&nbsp;Moreover, while bird pollination seems to be the end point in the evolution of pollination syndromes in many angiosperm lineages, rodent pollinated flowers probably evolved from ancestral bird pollinated flowers in Loasoideae. Our findings suggest that the evolution of bird pollinated flowers from ancestral bee pollinated flowers does not require the presence of corolla tubes or spurs, and can take place as long as the flower design includes structures participating in flower?pollinator fit.","palabras_clave":"LOASOIDEAE|POLLINATOR SELECTION|HUMMINGBIRD POLLINATION|BEE POLLINATION|FLOWER SHAPE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4210,"idioma_id":4,"titulo":"Study of abrasive wear resistance of Fe-based nanostructured hardfacing","resumen":"In the last years several consumables which deposit hard nanostructured iron-based alloys with high resistanceto abrasive wear have been developed. The microstructure of the weld metal usually shows variations with thewelding procedure, particularly related to the heat input, number of layers and shielding gas type. The purposeof this work was to study the microstructural evolution and wear resistance of a nanostructured iron-based alloydeposited by FCAW process. Four samples with one and two layers were deposited under Ar-20%CO2 shieldingand without shielding gas, using a heat input of 3.5 kJ\/mm. For each condition chemical composition wasdetermined and microstructure was studied using both optical and electronic microscopy and X ray diffraction.Microhardness and abrasive wear resistance were measured. The microhardness of the deposit was foundbetween 780 and 1020 HV2, depending on the number of layers. There was a variation of the chemicalcomposition between the first and the second layer. The wear test results were discussed in relation to chemicalcomposition, microstructure and microhardness.","palabras_clave":"HARDFACING|NAOMATERIALS|NUMBER OF LAYERS|MICROSTRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4211,"idioma_id":5,"titulo":"Diagnostico molecular de leptospiras patogenas en orina bovina mediante una PCR duplex","resumen":"La leptospirosis es la zoonosis de mayor distribución mundial yes endémica en países tropicales y subtropicales. En nuestro país, estazoonosis produce pérdidas importantes en la producción bovina ya que seestima que el 10% de los abortos son producidos por leptospirosis. Elobjetivo de este estudio fue comparar la eficiencia de tres diferentesmétodos de extracción de ADN a partir de orina bovina para la posteriordetección de Leptospira spp. mediante una PCR dúplex. Para ello, seanalizaron alícuotas de orina bovina inoculadas con distintas cantidadesde bacterias de la cepa L. interrogans serovar Pomona Pomona (partir deun cultivo de 1 x 108\/ml), las cuales fueron extraídas mediante (1) unkit comercial, (2) una resina quelante y (3) un método de hervido ycentrifugación. El método con mejores resultados fue el kit comercial,que permitió la detección de 10 leptospiras por ml de orina, seguido delmétodo 3 (hervido y centrifugación) permitió la concentración más alta deADN extraído y la detección de 1x 103 leptospiras por ml. Esta técnicatiene un gran potencial para el diagnóstico molecular de la leptospirosisen bovinos a partir de muestras de orina y aporta una herramienta de granutilidad para estudios epidemiológicos de esta enfermedad.","palabras_clave":"DIAGNOSTICO|LEPTOSPIROSIS|ORINA|BOVINOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4212,"idioma_id":4,"titulo":"Fibrin(ogen)olytic and antiplatelet activities of a subtilisin- like protease from Solanum tuberosum (StSBTc-3)","resumen":"Plant serine proteases have been widely used in food science and technology as well as in medicine. Inthis sense, several plant serine proteases have been proposed as potential anti-coagulants and antiplateletagents. Previously, we have reported the purification and identification of a plant serine proteasefrom Solanum tuberosum leaves. This potato enzyme, named as StSBTc-3, has a molecular weight of72 kDa and it was characterized as a subtilisin like protease. In this work we determine and characterizethe biochemical and medicinal properties of StSBTc-3. Results obtained show that, like the reported toother plant serine proteases, StSBTc-3 is able to degrade all chains of human fibrinogen and to producesfibrin clot lysis in a dose dependent manner. The enzyme efficiently hydrolyzes b subunit followed bypartially hydrolyzed a and g subunits of human fibrinogen. Assays performed to determine StSBTc-3substrate specificity using oxidized insulin b-chain as substrate, show seven cleavage sites: Asn3-Gln4;Cys7-Gly8; Glu13-Ala14; Leu15-Tyr16; Tyr16-Leu17; Arg22-Gly23 and Phe25-Tyr26, all of them werepreviously reported for other serine proteases with fibrinogenolytic activity. The maximum StSBTc-3fibrinogenolytic activity was determined at pH 8.0 and at 37 C. Additionally, we demonstrate that StSBTc-3 is able to inhibit platelet aggregation and is unable to exert cytotoxic activity on human erythrocytesin vitro at all concentrations assayed. These results suggest that StSBTc-3 could be evaluated as a newagent to be used in the treatment of thromboembolic disorders such as strokes, pulmonary embolismand deep vein thrombosis.","palabras_clave":"PLANT SERINE PROTEASES|ANTICOAGULANT AND ANTITHROMBOTIC AGENTS|PLATELETS|HAEMOSTASIS|THROMBOLYTIC THERAPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4213,"idioma_id":4,"titulo":"Environmental harshness decreases ant &#946;-diversity between salt marsh and neighboring upland environments","resumen":"Understanding how wetland organisms interactwith neighbor habitats along environmental gradients is importantto recognize wetland integrity and its connectivity atlandscape-level.We evaluated whether assemblage characteristics(e.g. á-diversity) of marsh-ants are associated with geographicchanges in environmental conditions, and if these factorsare associated with marsh-upland dissimilarity in ant speciescomposition (â-diversity). Ant-samples were collectedboth in the marsh and in the neighboring upland habitat at 5-sites along the South-west Atlantic (SWA) coastline (36°S to40°S), encompassing two-distinct biogeographic regions.Generalized Linear Models showed that at the marsh scale,ant occurrence increased with maximum temperature andSpartina densiflora cover, but decreased with total-plant cover.Ant richness increased with salinity, S. densiflora cover andmarsh area; and ant á-diversity increased with S. densifloracover and decreased with total marsh plant cover and plantheight. Composition of ant assemblages differed between themarsh and the upland habitat depending on the site, and â-diversity decreased with precipitation, salinity, tidal amplitudeand á-diversity of the herbaceous stratum. Then, the abundanceand á-diversity of ants varied along SWA marshes inrelation to changes in local environmental factors and the regionallandscape. Moreover, changes in species characteristicsacross coastal-landscape seem to interact with environmentalgradients, resulting in reduced â-diversity values with increasingenvironmental harshness. Thus, our results suggest thatthe link of geographic changes in the physical environmentwith the changes in species traits drives the variation in marshuplanddissimilarity across the space","palabras_clave":"diversity|ants|saltmarsh|environments"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4214,"idioma_id":4,"titulo":"Draft genome of Shewanella frigidimarina Ag06-30, a marine bacterium isolated from Potter Peninsula, King George Island, Antarctica","resumen":"We present the draft genome of Shewanella frigidimarina Ag06-30, a marine bacterium from King George Island, Antarctica, which encodes the carbapenemase SFP-1. The assembly contains 4,799,218 bp (GC content 41.24%). This strain harbors several mobile genetic elements that provide insight into lateral gene transfer and bacterial plasticity and evolution.","palabras_clave":"ANTARCTIC BACTERIA|SHEWANELLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4215,"idioma_id":4,"titulo":"Spatial Inferences in Narrative Comprehension: the Role of Verbal and Spatial Working Memory","resumen":"During the comprehension of narrative texts, readers keep a mental representation of the location of protagonists and objects; a breach in spatial coherence is detected by longer online reading times (consistency effect). We addressed whether these spatial inferences involve verbal or spatial working memory in two experiments, combining the consistency paradigm with selective verbal and spatial working memory concurrent tasks. The first experiment found longer reading times with a concurrent spatial task under imagery instructions (t33 = 2.87, p = .021). The second experiment, under comprehension reading instructions, found effects of verbal interference on reading times and accuracy. With a verbal secondary task, reading times for the target sentence were shorter (t45 = 3.60, p = .004) and the error rate was significantly higher (t47 = 2.95, p = .005) than without interference. This pattern of results suggests that spatial inferences in narrative comprehension rely mainly on verbal resources, and spatial working memory resources are recruited when imagery is required.","palabras_clave":"spatial inferences|narrative comprehension|discourse processing|working memory"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4216,"idioma_id":5,"titulo":"Biomineralización de cobre en Candida fukuyamaensis RCL-3","resumen":"Irazusta V., Michel L. & L.I.C. de Figueroa (2016).Biomineralización de cobre en Candida fukuyamaensis RCL-3. Revista Argentina de Microbiología, ISSN 0325-7541, 48, 166-170.","palabras_clave":"Biomineralización|Cobre| Candida fukuyamaensis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4217,"idioma_id":4,"titulo":"Nucleation of plasticity in nanoparticle collisions","resumen":"While at small collision velocities, collisions of nanoparticles (NPs) are elastic, they become plastic at higher velocities. We study the elastic-plastic threshold and the onset of plasticity using molecular dynamics simulation for a Lennard-Jones material. The threshold velocity increases as R^{-2\/3} for small NP radii R; this corrects  the often used assumption of a size-independent critical velocity.  At the threshold, NP orientation  strongly influences the generation of plasticity and averaging over many orientations is required to predict the critical velocity for dislocation generation. The onset of plasticity is governed by the generation of isolated stacking faults and nanotwins spanning the entire NP. At higher velocities, the fraction of defects becomes proportional to the total number of atoms in the NP.","palabras_clave":"CLUSTER COLLISIONS|MOLECULAR DYNAMICS|NANOPARTICLES|PLASTICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4218,"idioma_id":4,"titulo":"Noise exposure of immature rats can induce different age-dependent extra-auditory alterations that can be partially restored by rearing animals in an enriched environment","resumen":"It has been previously shown that different extra-auditory alterations can be induced in animals exposed to noise at 15 days. However, data regarding exposure of younger animals, that do not have a functional auditory system, have not been obtained yet. Besides, the possibility to find a helpful strategy to restore these changes has not been explored so far. Therefore, the aims of the present work were to test age-related differences in diverse hippocampal-dependentbehavioral measurements that might be affected in noise-exposed rats, as well as to evaluate the effectiveness of a potential neuroprotective strategy, the enriched environment (EE), on noise-induced behavioral alterations. Male Wistar rats of 7 and 15 days were exposed to moderate levels of noise for two hours. At weaning, animals were separated and reared either in standard or in EE cages for one week. At 28 days of age, different hippocampal-dependent behavioral assessments were performed. Results show that rats exposed to noise at 7 and 15days were differentially affected. Moreover, EE was effective in restoring all altered variables when animals were exposed at 7 days, while a few were restored in rats exposed at 15 days. The present findings suggest that noise exposure was capable to trigger significant hippocampal-related behavioral alterations that were differentially affected, depending on the age of exposure. In addition, it could be proposed that hearing structures did not seem to be necessarily Involved in the generation of noise-induced hippocampal-related behaviors, asthey were observed even in animals with an immature auditory pathway. Finally, it could be hypothesized that the differential restoration achieved by EE rearing might also depend on the degree of maturation at the time of exposure and the variable evaluated, being younger animals more susceptible to environmental manipulations.","palabras_clave":"NOISE|BEHAVIOR|ENRICHED ENVIRONMENT|CENTRAL NERVOUS SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4219,"idioma_id":4,"titulo":"Positional stability and radial dynamics of sonoluminescent bubbles under bi-harmonic driving: Effect of the high-frequency component and its relative phase","resumen":"The use of bi-frequency driving in sonoluminescence has proved to be an effective way to avoid the spatial instability (pseudo-orbits) developed by bubbles in systems with high viscous liquids like sulphuric or phosphoric acids. In this work, we present extensive experimental and numerical evidence in order to assess the effect of the high frequency component (PAcHF) of a bi-harmonic acoustic pressure field on the dynamic of sonoluminescent bubbles in an aqueous solution of sulphuric acid. The present study is mainly focused on the role of the harmonic frequency (Nf0) and the relative phase between the two frequency components (&#966;b) of the acoustic field on the spatial, positional and diffusive stability of the bubbles. The results presented in this work were analyzed by means of three different approaches. First, we discussed some qualitative considerations about the changes observed in the radial dynamics, and the stability of similar bubbles under distinct bi-harmonic drivings. Later, we have investigated, through a series of numerical simulations, how the use of high frequency harmonic components of different order N &nbsp;, affects the positional stability of the SL bubbles. Furthermore, the influence of &#966;b&#966;b in their radius temporal evolution is systematically explored for harmonics ranging from the second to the fifteenth harmonic (N=2 to&nbsp;N=15). Finally, a multivariate analysis based on the covariance method is performed to study the dependences among the parameters characterizing the SL bubble. Both experimental and numerical results indicate that the impact of PAcHF on the positional instability and the radial dynamics turns to be progressively negligible as the order of the high frequency harmonic component grows (i.e. N&#8811;1), however its effectiveness on the reduction of the spatial instability remains unaltered or even improved.","palabras_clave":"Sonoluminescence (SBSL)|Bi-harmonic driving|Positional instability|Bjerknes force|Relative phase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4220,"idioma_id":4,"titulo":"By-product from pumpkin (Cucurbita Moschata Duchesne ex Poiret) as a substrate and vegetable matrix to contain Lactobacillus casei","resumen":"Pumpkin waste was used as a substrate and support of Lactobacillus casei (ATCC-393). A complete factorial design was performed in order to study the effect of water content, whey,inoculum concentration and the incubation period on cell growth and viability. According to the results of the experimental design, L. casei could use pumpkin waste as a substrate successfully and the system with 10 mL\/g of water and without whey was selected to continue with further assays.The kinetics of the L. casei growth were determined.The maximum specific growth rate was 0.6 h&#8722;1. After washing, centrifugation, and vacuum drying, 87% of viable cells were recovered. L. casei adhered to pumpkin powder was tested as a dietary supplement in chocolate milk or in a soy and apple juice based beverage. Approximately 80% of cells resisted gastrointestinal simulated conditions. Both supplemented beverages were well accepted by consumers.","palabras_clave":"LACTOBACILLUS CASEI|PROBIOTICS|FUNCTIONAL FOODS|PUMPKIN|BY-PRODUCTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4221,"idioma_id":4,"titulo":"Phase diagrams for the adsorption of monomers with non-additive interactions","resumen":"In several experimental systems phase diagrams coverage-temperature show a strongasymmetry. This behavior can be reproduced by including non-additive lateral interactions.In this work a Monte Carlo study on the canonical assembly of the criticalityof monomer adsorption with non-additive interactions is presented. Traditionalpairwise energies were replaced by other more general ones where the lateral interactionbetween two ad-atoms depends on the coverage at first sphere of coordination.These energies induce the formation of several non-additive ordered structures.Finite size scaling method was used to classify the order of phase transitionof each non-additive phase. On the other hand, the corresponding phase diagramsare formed naturally, in which case the diagrams show strong asymmetries","palabras_clave":"Computer simulations|Monte Carlo simulations|Equilibrium thermodynamics|Statistical mechanics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4222,"idioma_id":4,"titulo":"SIZE-DEPENDENT COMPLEX DIELECTRIC FUNCTION OF Ni, Mo, W, Pb, Zn and Na NANOPARTICLES.  APPLICATION TO SIZING","resumen":"This work determines the size dependent metal nanoparticle (NP) dielectric function from a ´top-down´ approach using the bulk experimental refractive index as a starting point. Free-electron damping constant (gamma_free) and plasma frequency (w_p) parameters in the Drude model are calculated for nickel (Ni), molybdenum (Mo), tungsten (W), lead (Pb), zinc (Zn) and sodium (Na) using a method developed in our group. Determined (gamma_free) and (w_p) parameters allow to develop an expression that improves the precision in reproducing the discrete metal bulk dielectric function in a wide wavelength range (UV-FIR). The bulk dielectric function is modified for describing the nanometric case by adding size corrective terms for free and bound electrons contributions. As an application of this study we characterize Ni spherical NPs synthesized by ultrafast laser ablation of a solid target in water. Using Mie theory together with the size-dependent dielectric function, we theoretically reproduce its experimental extinction spectrum. From this fitting, composition and size distribution of the particles in the colloidal suspension may be derived. Transmission electron microscopy (TEM) results agree with the sizes and structure derived from optical extinction spectroscopy (OES).","palabras_clave":"COMPLEX DIELECTRIC FUNCTION|METAL NANOPARTICLES|PLASMA FREQUENCY|DAMPING CONSTANT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4223,"idioma_id":5,"titulo":"Investigación socio-ecológica a largo plazo en la Patagonia Austral: Estrategias interdisciplinarias para lograr la conservación de los recursos naturales a través de un manejo sustentable bajo escenarios de cambio global","resumen":"Las dimensiones ecológicas, sociales, institucionales y políticas de los problemas ambientales abarcan escalas espaciales y temporales que excedena la mayoría de las investigaciones científicas. Una alternativa que crece con el tiempo a nivel mundial es la aproximación Long-Term Ecological Research (LTER) o Long-Term Socio-Ecological Research (LTSER), pero que presenta vacíos temáticos y geográficos. Uno de estos vacíos es la Argentina, sin embargo, existe una gran cantidad de experiencia para poder integrar potencialmente al país a dicha red. El objetivo de este trabajo es evaluar el marco general de la investigación ecológica a largo plazo que se realiza en Argentina, y particularmente en la Patagonia Austral, tomamos dos líneas de trabajo como estudios de caso: (i) la ecología y conservación de los bosques nativos a través del manejo forestal y silvopastoril sostenible, y (ii) la ecología y el manejo de las invasiones biológicas. Se presenta una reseña de las investigaciones realizadas (en el pasado y al presente), sus resultados, las estrategias aplicadas en el pasado y las lecciones aprendidas. Esta reseña permite inferir acerca de la factibilidad de implementar esta aproximación en la Argentina, y que el mismo pueda ser sostenidos en el tiempo y que sea integrados entre distintas disciplinas y tomadores de decisiones. Se concluye que existe un potencial actual y grandes oportunidades a futuro para poder consolidar una red del tipo LTER\/LTSER en Argentina.","palabras_clave":"MANEJO FORESTAL|USO SILVO-PASTORIL|DIMENSION SOCIO-ECOLOGICA|ESPECIES INVASORAS|RED DE PARCELAS PERMANENTES DE LARGO PLAZO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4224,"idioma_id":5,"titulo":"Enfoques de Aprendizaje en estudiantes universitarios argentinos según el R-SPQ-2F: Análisis de sus propiedades psicométricas","resumen":"Se presenta la adaptación del Cuestionario Revisado de Procesos de Estudio, Dos Factores R-SPQ-2F, que evalúa enfoques de aprendizaje, para su uso en población estudiantil universitaria argentina. Tres jueces expertos analizaron aspectos de validez de contenido y 20 estudiantes valoraron adecuación lingüística y evidencias de validez aparente. Se recogieron además dos muestras independientes para analizar evidencias de validez de constructo: 204 estudiantes de 18 a 36 años (  = 23.57; DE = 3.67) para el estudio factorial exploratorio y de consistencia interna, y 528 alumnos entre 17 y 36 años (  = 22.50; DE = 3.73) para el análisis factorial confirmatorio, que comparó tres modelos mediante máxima verosimilitud robusto. El estudio de confiabilidad test-restest incluyó 19 educandos. Los resultados indican mejor ajuste del modelo de dos factores que representa los enfoques de aprendizaje profundo y superficial. Se discuten los hallazgos a la luz de la teoría y de implicaciones prácticas.","palabras_clave":"R-SPQ-2F|VALIDEZ|ESTUDIANTES UNIVERSITARIOS|ENFOQUES DE APRENDIZAJE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4225,"idioma_id":4,"titulo":"Discovery of a deep Seyfert-2 galaxy at z = 0.222 behind NGC 300","resumen":"We report on the unveiling of the nature of the unidentified X-ray source 3XMM J005450.3?373849 as a Seyfert 2 galaxy located behind the spiral galaxy NGC 300 using Hubble Space Telescope data, new spectroscopic Gemini observations and available XMM-Newton and Chandra data. We show that the X-ray source is positionally coincident with an extended optical source, composed by a marginally resolved nucleus\/bulge, surrounded by an elliptical disc-like feature and two symmetrical outer rings. The optical spectrum is typical of a Seyfert 2 galaxy redshifted to z = 0.222 +\/- 0.001, which confirms that the source is not physically related to NGC 300. At this redshift the source would be located at 909 +\/- 4 Mpc (comoving distance in the standard model). The X-ray spectra of the source are well-fitted by an absorbed power-law model. By tying NH between the six available spectra, we found a variable index Gamma running from ~2 in 2000-2001 years, to 1.4?1.6 in the 2005-2014 period. Alternatively, by tying Gamma, we found variable absorption columns of NH &#8764; 0.34 x 10^{-22} cm^{-2} in 2000-2001 years, and 0.54 - 0.75 x 10^{-22} cm^{-2} in the 2005-2014 period. Although we cannot distinguish between an spectral or absorption origin, from the derived unabsorbed X-ray fluxes, we are able to assure the presence of long-term X-ray variability. Furthermore, the unabsorbed X-ray luminosities of 0.8 - 2x10^{43} erg s^{-1} derived in the X-ray band are in agreement with a weakly obscured Seyfert-2 AGN at z = 0.22.","palabras_clave":"galaxies: Seyfert|X-rays: galaxies|X-rays: individual: 3XMM J005450.3-373849|X-rays: individual: XMMU J005450.0-373853"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4227,"idioma_id":4,"titulo":"Vitrification of immature and matured bovine oocytes: effect of brilliant cresyl blue selection and hyaluronan addition","resumen":"The aim of the present study was to evaluate the effect of brilliant cresyl blue (BCB) selection, the type of oocyte (immature or matured) and the use of hyaluronan in the vitrification solution on further embryo developmental competence. Oocytes (n = 1308) obtained from abattoir ovaries were classified by BCB stain. Control oocytes were maintained in holding media for 90 min and then subdivided to be placed into maturation media without any treatment or were vitrified. Immature or matured oocytes were vitrified by the solid-surface technique using two different vitrification solutions. VS1: composed of 10% ethylene glycol (EG) for 10 min followed by 20% EG + 0.2M trehalose for 30 sec and finally into 30% EG + 0.5M trehalose for 30 sec, or VS2 composed by 10% EG for 10 min, followed by 20% EG + 0.2M trehalose for 30 sec, and finally into 30% EG+ 0.5M trehalose + 0.1 g\/ml hyaluronan for 30 sec. Oocytes were then loaded into Fyberplugs? and vitrified. After one week, Fyberplugs? were open and placed directly into (37°C) 0.5M sucrose solution for 5 min, then into 0.25M of sucrose for another 5 min and finally placed into maturation medium for in vitro production. Cleavage and development rates were examined on days 2 and 7 after fertilization, respectively","palabras_clave":"oocytes|vitrification|brilliant cresyl blue|bovine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4228,"idioma_id":5,"titulo":"¿Poblaciones superfluas? Estrategias asociativas en luchas locales emergentes en respuesta a problemas globales.","resumen":"Este artículo presenta los resultados parciales de la investigación Movimientos sociales que resisten la exclusión: alternativas y dificultades en procesos de participación y transformación ciudadanas?. Código: 20020130100830BA Programación científica UBACyT 2014-2017, con sede en el Instituto de Investigaciones de la Facultad de Psicología de la Universidad de Buenos Aires. Dicha investigación se orienta a analizar las estrategias asociativas que establecen los grupos y los movimientos que resisten la exclusión, y que dan a su vez expresión a su responsabilidad política y ciudadana. El tipo de estudio es de carácter exploratorio y descriptivo recurre a la investigación - acción participativa para promover el desarrollo de las praxis transformadora con rigor científico y sensibilidad social. Dichas coordenadas brindan el marco de análisis que este trabajo expone acerca de dos casos de investigación en curso: la metalúrgica recuperada por sus trabajadores y trabajadoras  IMPA y la cooperativa de promotores ambientales El Correcaminos.","palabras_clave":"EXCLUSIÓN|RESISTENCIA|SUBJETIVIDADES|MENTALIDADES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4229,"idioma_id":4,"titulo":"Spinodals and critical point using short-time dynamics for a simple model of liquid","resumen":"We have applied the short-time dynamics method to the gas-liquid transition to detect the supercooled gas instability (gas spinodal) and the superheated liquid instability (liquid spinodal). Using MonteCarlo simulation, we have obtained the two spinodals for a wide range of pressure in sub-critical and critical conditions and estimated the critical temperature and pressure. Our method is fasterthan previous approaches and allows studying spinodals without needing equilibration of the system in the metastable region. It is thus free of the extrapolation problems present in other methods,and in principle could be applied to systems such as glass-forming liquids, where equilibration is very difficult even far from the spinodal. We have also done molecular dynamics simulations,where we find the method again able to detect the both spinodals. Our results are compared with different previous results in the literature and show a good agreement.","palabras_clave":"liquid thermodynamics|spinodal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4230,"idioma_id":4,"titulo":"Comparison of the efficiency of 5 methods for fungal DNA extraction from crop debris and evaluation of its suitability for the amplification of Fusarium graminearum by PCR","resumen":"Quantification of fungal DNA in crop debris by PCR could contribute to the development of strategies aimed at managing plant diseases. Five protocols were tested for its efficiency in the isolation of Fusarium graminearum DNA from crop debris. A CTAB-based method provided the best results (free of inhibitors) as confirmed by conventional and qPCR.","palabras_clave":"Fusarium head blight|Gibberella zeae|Real TIme PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4231,"idioma_id":4,"titulo":"Telomerase as a cancer target. Development of new molecules","resumen":"Telomeres are the terminal part of the chromosome containing a long repetitive and noncodifying sequence that has as function protecting the chromosomes. In normal cells, telomeres lost part of such repetitive sequence in each mitosis, until telomeres reach a critical point, triggering at that time senescence and cell death. However, in most of tumor cells in each cell division a part of the telomere is lost, however the appearance of an enzyme called telomerase synthetize the segment that just has been lost, therefore conferring to tumor cells the immortality hallmark. Telomerase is significantly overexpressed in 80-95% of all malignant tumors, being present at low levels in few normal cells, mostly stem cells. Due to these characteristics, telomerase has become an attractive target for new and more effective anticancer agents. The capability of inhibiting telomerase in tumor cells should lead to telomere shortening, senescence and apoptosis. In this work, we analyze the different strategies for telomerase inhibition, either in development, preclinical or clinical stages taking into account their strong points and their caveats. We covered strategies such as nucleosides analogs, oligonucleotides, small molecule inhibitors, G-quadruplex stabilizers, immunotherapy, gene therapy, molecules that affect the telomere\/ telomerase associated proteins, agents from microbial sources, among others, providing a balanced evaluation of the status of the inhibitors of this powerful target together with an analysis of the challenges ahead.","palabras_clave":"CANCER|TELOMERASE|INHIBITOR|TARGET"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4232,"idioma_id":4,"titulo":"CHALLENGES IN PROTEIN FORMULATION FOCUSED","resumen":"Biotechnology revolution had led the overcoming of different types of therapeutic protein because of their chemical structure can perform specific reactions in the body, increasing efficacy and decreasing side effects. Numerous efforts were made to optimize the physicochemical properties of the proteins used for therapeutic and studied different methods for an effective administration of the protein contained in the medicine, evaluating different routes of administration to achieve the desired therapeutic effects. The delivery system for oral pharmaceutical proteins and peptides is still in development stage. There are number of limitations to oral delivery of proteins such as barriers to peptide bioavailability after oral administration, intestinal membrane permeability, size, intestinal and hepatic metabolism and solubility. Pellets have shown great potential in the delivery of proteins\/peptidal drugs. Some strategies of development of oral protein and peptides has always been challenged, optimizing the safety and efficacy while ensuring the ability to manufacture the drug while maintaining quality and stability. The pelletization techniques have been reviewed in numerous papers, is a technique that enables the formation of spherical beads or pellets with a mean diameter usually ranging from 0.5-2.0 mm. Pellets are prepared by different techniques, such as extrusion and spheronization. This review discusses challenges in protein formulation that have been used to prepare pelletized dosage forms using the extrusion-spheronization process.","palabras_clave":"DRUG DELIVERY|MULTIPLE-UNIT SYSTEM|EXTRUSION-SPHERONIZATION|PELLETS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4233,"idioma_id":5,"titulo":"El período de repago de las inversiones productivas en Argentina ? Evidencia obtenida de la evolución del PBI 1950 ? 2008","resumen":"El período de repago mide la exposición al riesgo del inversor y es una de las métricas que se usan para evaluarinversiones, junto con la TIR y el VAN. En este caso hemos considerado que el inversor típico local, que se financiaen el mercado interno, analiza y proyecta la historia económica del país y decide aceptar inversiones que, almenos, no estén en riesgo de caer dentro de una crisis. Es decir, el inversor típico tiene preferencia por la liquidezen vista de las sucesivas crisis económico-financieras del país. En este caso hemos considerado la serie temporalde PBI anual como un modelo autorregresivo para dos estados de una cadena de Markov oculta (crecimiento orecesión). Los gráficos de PBI a lo largo del período considerado, muestran frecuentes cambios de régimen quepermiten calcular las probabilidades de transición de la Cadena deMarkovOculta que gobierna dichos cambios.Estas probabilidades se calculan suponiendo que la única variable independiente es el tiempo, expresado en años.Usamos para los cálculos la serie de PBI, en dólares constantes de 2000, publicada porCEPAL(1). Lamotivaciónde este trabajo es la búsqueda de las causas del escaso crecimiento en el período considerado, crecimiento que depende,tanto como Inversión Extranjera Directa (IED) como de la inversión de empresas locales. La IED, por suparte, amenudo reclama un tratamiento de excepción en aspectos impositivos y cambiarios, para poder asegurara sus accionistas una rentabilidad razonable. Las probabilidades mencionadas se estimaron por el estimador deMáxima Verosimilitud siguiendo la metodología de Hamilton","palabras_clave":"Argentina|Producto Bruto Interno|Transiciones de Markov"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4234,"idioma_id":5,"titulo":"Conocimiento y valoración de la biodiversidad en estudiantes del último  año de profesorados de biología y geografía de Argentina","resumen":"El objetivo del presente trabajo fue explorar los conocimientos y valoración queposee sobre biodiversidad una muestra de estudiantes del último año de los Profesorados en Ciencias Biológicas y en Geografía de la provincia de Buenos Aires, Argentina. El estudio se basó en el análisis de un cuestionario de cinco ítems, que tuvo en cuenta el concepto de biodiversidad, los benefcios que ésta posee, las causas y consecuencias de su degradación y los ambientes de mayor biodiversidad de la argentina. A través de los resultados obtenidos, fue posible determinar que los estudiantes expresan conceptos simplifcados, reducidosprincipalmente a la diversidad específca, con una mirada ecológica y antropocéntrica. Se destaca la escasa consideración de los aspectos socio-culturales de la biodiversidad. Si bien se consideran las principales causas de pérdida de biodiversidad, la introducción de especies exóticas es muy poco considerada.","palabras_clave":"Biodiversidad|Formación docente|Ciencias Biológicas|Geografía"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4235,"idioma_id":4,"titulo":"Impacts of Crop Residue on Damage by Sugarcane Pests During the Tillering Phase in Argentina","resumen":"One of the most important recent changes in sugarcane cultural practices in Tucumán, Argentina, is the adoption of the practice of green-cane harvesting, which involves harvesting the sugarcane crop in the absence of burning. The objective of this study was to assess the impact of the postharvest crop residue (also sometimes known as ?trash blanket?) on Elasmopalpus lignosellus (Zeller) (Lepidoptera: Pyralidae) and Pseudaletia unipuncta Haworth (Lepidoptera: Noctuidae) population dynamics in sugarcane. The study was conducted in ratoon crops for 3 growing seasons (2011, 2012, and 2013) and at 3 locations in the state of Tucumán, Argentina. The treatments consisted of rows with the crop residue burned and the crop  residue retained. Removal of the crop residue by burning, compared with retention, resulted in significantly greater crop damage by E. lignosellus in all locations and years. In contrast, damage by P. unipuncta was observed exclusively on plots where crop residue was retained. Comparing the pest status of 2 insects, E. lignosellus seems to be more consistently deleterious to sugarcane yield than P. unipuncta, and chemical control of this species is not very effective. Therefore, leaving the crop residue in place seems to be the most appropriate crop management approach, although it is important also to monitor P. unipuncta populations in order to implement control should it be necessary","palabras_clave":"TRUE ARMYWORM |LESSER CORMSTALK BORER|GREEN HARVEST|TRASH BLANKET|STUBBLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4236,"idioma_id":4,"titulo":"A Bayesian Technique for Real and Integer Parameters Estimation in Linear Models and its Application to GNSS High Precision Positioning","resumen":"A novel Bayesian technique for the joint estimation of real and integer parameters in a linear measurement model is presented. The integer parameters take values on a finite set, and the real ones are assumed to be a Gaussian random vector. The posterior distribution of these parameters is sequentially determined as new measurements are incorporated. This is a mixed distribution with a Gaussian continuous part and a discrete one. Estimators for the integer and real parameters are derived from this posterior distribution. A Maximum A Posteriori (MAP) estimator modified with the addition of a confidence threshold is used for the integer part and a Minimum Mean Squared Error (MMSE) is used for the real parameters. Two different cases are addressed: i) both real and integer parameters are time invariant and ii) the integer parameters are time invariant but the real ones are time varying. Our technique is applied to the GNSS carrier phase ambiguity resolution problem, that is key for high precision positioning applications. The good performance of the proposed technique is illustrated through simulations in different scenarios where different kind of measurements as well as different satellite visibility conditions are considered. Comparisons with state-of-the-art ambiguity solving algorithms confirm performance improvement. The new method is shown to be useful not only in the estimation stage but also for validating the estimates ensuring a predefined success rate through proper threshold selection.","palabras_clave":"Bayesian estimation|Carrier-phase ambiguity resolution|GNSS|integer parameter estimation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4237,"idioma_id":4,"titulo":"Effect of polyploidization in the production of essential oils in Lippia integrifolia","resumen":"tConsumption of medicinal and aromatic plants is widespread and increasing worldwide. Yet, harvestingfrom the wild, the main source of raw material in developing countries, is causing loss of genetic diversityand habitat destruction. This situation makes imperative the development and application of breedingprograms. Autopolyploidy has brought advantages for the improvement of agronomic traits of economi-cally important plants. In this sense, obtaining polyploid individuals is an interesting strategy to achievethis objective. In the present study successful induction of polyploidy in Lippia integrifolia (?incayuyo?)was achieved by applying colchicine in the multiplication medium MS + 2.2 M BAP. Induced autote-traploids showed significant differences from the field mother plant (size of the leaves, inflorescences,trichomes, stomatas, and pollen grains). In addition, essential oil yields were enhanced in tetraploids,and surprisingly, quantitative differences were detected in the composition of all recovered individualsfrom in vitro culture (tetraploids and diploids) with respect to the mother field plant. Due to the phe-notypic differences, and enhanced essential oil yields and composition, tetraploid individuals became anew variety of incayuyo.","palabras_clave":"Polyploidization|Colchicine|Tissue Culture|Autotetraploids|Essential oils"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4238,"idioma_id":4,"titulo":"Essential oils of native and naturalized Lamiaceae species growing in the Patagonia region (Argentina)","resumen":"This is the first comprehensive study of essential oils of wild aromatic Lamiaceae present in Patagonia, including exotic and native species. The most commonly representatives of this family in Patagonia include one species of the adventitious Origanum vulgare L., three naturalized mints, Mentha rotundifolia L., M. pulegium L. and M. spicata L. and two native species: Clinopodium darwinii (Benth.) Kuntze and Scutellaria nummulariifolia Hook. f. The essential oils were isolated by hydrodistillation of the aerial parts of each of these species collected during 3 years and analyzed by GC-FID-MS. The major components found were menthone (5.4?10.2%) and pulegone (78.3?89.8%) in M. pulegium; piperitenone oxide (75.6?68.7%) in M. rotundifolia; carvone (34.0?70.4%) in M. spicata; sabinene (2.7?5.9%), p-cymene (9.3?36.1%) and linalool (11.3?21.4%) in O. vulgare; pulegone (56.6?72.4%) in Clinopodium darwinii; acetophenone (19.0%) and &#945;-terpineol (9.5%) in Scutellaria nummulariifolia. This is the first report on the chemical composition of the essential oil of S. nummulariifolia.","palabras_clave":"Lamiaceae|Patagonia|essential oils|Scutellaria nummulariifolia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4239,"idioma_id":5,"titulo":"Actividad antioxidante en infusiones, tinturas y aceites esenciales de especies nativas de la Patagonia Argentina","resumen":"ResumenIntroducción: las plantas aromáticas y medicinales son una fuente potencial de componentes antioxidantes. La Patagonia Argentina presenta un ambiente diverso en especies nativas, las cuales deberían ser estudiadas en mayor profundidad debido a su potencial farmacéutico, así como para contribuir a fomentar su conservación. Objetivos: estudiar la actividad antioxidante de infusiones, tinturas y aceites esenciales de las siguientes especies nativas de la Patagonia Argentina: Acantholippia seriphioides (A. Gray) Moldenke, Adesmia boronioides Hook. f., Buddleja globosa Hope, Fabiana imbricata Ruiz & Pav., Solidago chilensis Meyen. Identificar los componentes volátiles presentes en los aceites esenciales. Métodos: se obtuvieron infusiones y tinturas por la guía de las normas de la Farmacopea Argentina VI edición. Los aceites esenciales se obtuvieron por hidrodestilación con un aparato tipo Clevenger. El análisis de los componentes volátiles se realizó mediante cromatografía de gases-espectrometría de masas. Los ensayos de actividad antioxidante se realizaron por el método del difenil-picrilhidrazilo. Resultados: las especies presentaron el siguiente orden de actividad antioxidante: B. globosa > S. chilensis &#8805; F. imbricata &#8805; A . seriphioides > A. boronioides. Las infusiones de B. globosa, S. chilensis y A. seriphioides, presentaron una actividad antioxidante similar a Camellia sinensis (L.) Kuntze (?té verde?) y superior a Ginkgo biloba (L.) Mant (especies reconocidas por su alto contenido de antioxidantes). La actividad encontrada para el aceite esencial de A. seriphioides se deba a sus contenidos en timol y carvacrol. En cuanto a la actividad de S. chilensis podría adjudicarse a su alto porcentaje de limoneno. Conclusiones : este trabajo es el primero que estudia la actividad antioxidante de plantas medicinales y aromáticas en la región noroeste de la Patagonia Argentina; los resultados obtenidos demuestran que las especies estudiadas de dicha región son una fuente rica en compuestos antioxidantes y de potencial valor como suplemento dietario","palabras_clave":"ACTIVIDAD ANTIOXIDANTE|PLANTAS MEDICINALES|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4240,"idioma_id":4,"titulo":"THE ROLE OF AFM, HRTEM, SAXS AND OPTICAL SPECTROSCOPY IN SIZING Cu, Ag, Fe AND Ni NANOPARTICLES GENERATED BY ULTRAFAST LASER ABLATION OF SOLID TARGETS IN DIFFERENT LIQUID MEDIA","resumen":"This work deals with the generation of Cu, Ag, Fe and Ni nanoparticles (NPs) by femtosecond laser ablation in different media and the characterization of their structures, configurations and sizes using Atomic Force Microscopy (AFM), High Resolution Transmission Electron Microscopy (HRTEM), Optical Extinction Spectroscopy (OES) and Small Angle X-Ray Scattering (SAXS). Colloidal suspensions receive nowadays considerable attention due to their attractive physicochemical properties, which boost applications in catalysis, optics, electronics and biomedical areas based on their unique size-dependent optical, electrical and magnetic properties. Over the last few years, we have carried out a systematic work in producing, by top down methods, noble-metal (Cu and Ag) metallic NPs colloids [1], [2] and more recently magnetic NPs such as Fe and Ni [3]. In laser ablation, energy is deposited in a bulk material at a sufficient fast rate so that it breaks up and forms particles in the nanometric scale. The structure of the so-produced colloids includes a variety of morphologies like bare-core, core-shell and hollows NPs as well as aggregates including fractals. AFM appears as a suitable tool for 3D characterization with sub-nanometric resolution. AFM and TEM characterize dried samples while OES and SAXS characterize the NPs structuring in the colloid state. The observation of isolated NPs is shown in Fig. 1 for Ag. The formation of fractal structure is shown in Fig. 2 for Fe colloid. Self-similarity property is observed for Ag colloid through the invariance under scale transformation comparing AFM (inset in the lower panel of Fig. 3) with optical microscopy images shown in Fig. 3. Self-similarity was analyzed by applying box-counting method to the AFM images. Complementarily, TEM technique images a hollow Ni NP, as shown in Fig. 4. The size distribution and composition of the colloidal suspensions were derived from OES by fitting experimental extinction spectra using Mie theory, taking into account specific modifications of the complex metal dielectric function with size for free and bound electron contributions, as shown for Cu colloid in Fig. 5. We discuss the advantages, limits and complementarity of AFM and TEM techniques with OES optical spectroscopy and SAXS to characterize nanometric size colloids produced by femtosecond laser ablation.","palabras_clave":"nanoparticles|fractals"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4242,"idioma_id":4,"titulo":"Towards better Scrum learning using learning styles","resumen":"Considerable attention has been paid to teaching Scrum in software engineering education as an academic response to the software industry?s demands. In order to reinforce and strengthen the understanding of Scrum concepts, professors should personalize the learning process, catering for students? individual learning characteristics. To address this issue, learning styles become effective to understand students? different ways of learning. In this context, the meshing hypothesis claims that when both teaching and learning styles are aligned, the students? learning experience is enhanced. However, the literature fails to evidence support for the meshing hypothesis in the context of software engineering education. We aim to corroborate the meshing hypothesis by using teaching strategies matching the Felder?Silverman Learning Style Model in a Scrum course. Based on previous findings, we focused on the processing dimension of the model. To validate our approach, two experiments were conducted in an undergraduate software engineering course in the academic years 2013 and 2014. We provided students with a Scrum class by applying teaching strategies suiting students? learning style. Test results corroborate that students? outcomes improved when receiving the strategy that match their learning styles. Our data highlight opportunities for improving software engineering education by considering the students? learning preferences.","palabras_clave":"AGILE SOFTWARE DEVELOPMENT|SOFTWARE ENGINEERING EDUCATION|LEARNING STYLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4243,"idioma_id":4,"titulo":"Artificial Intelligence in Service-Oriented Software Design","resumen":"Service-Oriented Architecture (SOA) has gained considerable popularity for the development of distributedenterprise-wide applications within the software industry. The SOA paradigm promotes the reusability andintegrability of software in heterogeneous environments by means of open standards. Most softwarecompanies capitalize on SOA by discovering and composing services already accessible over the Internet,whereas other organizations need internal control of applications and develop new services with qualityattributeproperties tailored to their particular environment. Therefore, based on architectural and businessrequirements, developers can elaborate different alternatives within a SOA framework to design theirsoftware applications. Each of these alternatives will imply trade-offs among quality attributes, such asperformance, dependability and availability, among others. In this context, Artificial Intelligence (AI) canassist developers in dealing with service-oriented design with the positive impact on scalability andmanagement of generic quality attributes. In this paper, we offer a detailed, conceptualized and synthesizedanalysis of AI research works that have aimed at discovering, composing, or developing services. We alsoidentify open research issues and challenges in the aforementioned research areas. The results of thecharacterization of 69 contemporary approaches and potential research directions for the areas are also shown.It is concluded that AI has aimed at exploiting the semantic resources and achieving quality-attributeproperties so as to produce flexible and adaptive-to-change service discovery, composition, and development.","palabras_clave":"ARTIFICIAL INTELLIGENCE|SERVICE-ORIENTED DESIGN|WEB SERVICE DISCOVERY|WEB SERVICE COMPOSITION|WEB SERVICE DEVELOPMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4245,"idioma_id":5,"titulo":"Protocolo de atención a un paciente con diagnóstico de pulpitis irreversible","resumen":"El tratamiento endodontico implica la eliminación del tejido pulpar afectado por procesos inflamatorios producidos por causas bacterianas, físicas y\/o químicas que llevan a su remoción total para conservar el órgano dental como parte funcional del sistema estomatognático. La biopulpectomia es un procedimiento endodóntico que se realiza en casos de pulpas vitales con procesos inflamatorios irreversibles. Implica la eliminación, limpieza y conformación de la cavidad pulpar creando un ambiente óptimo para la obturación tridimensional del sistema de conductos radiculares. En esste artículo se brinda un protocolo de atención a pacientes con diagnostico de pulpitis irreversibles que concurren a la Cátedra de endodoncia de 5º año de la Facultad de Odontología de la UNNE.","palabras_clave":"cavidad pulpar|tratamiento endodontico|extirpación pulpar|endodoncia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4247,"idioma_id":4,"titulo":"Chemical composition and electronic structure of anodic passive films on low-C 13CrNiMo stainless steel","resumen":"Mott?Schottky analysis and electrochemical and Xray photoelectron spectroscopy (XPS) measurements were performed on passive filmsformed on lowC 13CrNiMo stainless steel with different applied heat treatments. Heat treatments render particular microstructural features ofthe alloy with a significant impact on the ability of the passive films to afford adequate protection against localized corrosion. A lower level of retained austenite in the substrate renders thinner passive films.Phosphates coexist with oxidized Fe(III) compounds as the prevailingspecies in the anodic layers. Mo was only detected in the oxide filmformed on the sample with a higher retained austenite content.Passive layers behave as ntype semiconductors with two types of donors, namely, shallowlevel and deeplevel states. The observed flat band potential V &#8773; 0.425 ± 0.005 V vs. standard calomel electrode (SCE) is independent of the thermal treatment of the alloy but under potential bias conditions at the corrosion potential the occurrence of thecathodic reaction on the oxide surface is hindered on the sample withhigher retained austenite in its microstructure as compared to the samplewith lower retained austenite content.","palabras_clave":"Alloys Passive films|Heat treatment|Stainless Steel|Passive films|Corrosion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4248,"idioma_id":4,"titulo":"Structural and spectroscopic study of a pectin isolated from citrus peel by using FTIR and FT-Raman spectra and DFT calculations","resumen":"'In this work, pectin isolated from citrus peel with a degree of esterification of 76% was characterized byFourier Transform Infrared (FTIR) and Fourier Transform Raman (FT-Raman) spectroscopies. Structuralstudies were carried out taking into account their partial degree of esterification and considering thepolygalacturonic acid chain as formed by two different subunits, one with both COOH and COO ACH3groups (Ac) and the other one as constituted by two subunits with two COO ACH3 groups (Es). Their structural properties, harmonic frequencies, force fields and force constants in gas and aqueous solutionphases were calculated by using the hybrid B3LYP\/6-31G &#8260; method. Then, their complete vibrational analyses were performed by using the IR and Raman spectra accomplished with the scaled quantum mechanical (SQM) methodology. Reactivities and behaviors in both media were predicted for Ac and Es by usingnatural bond orbital (NBO), atoms in molecules (AIM), and frontier orbitals calculations. We report forfirst time the complete assignments of those two different units of polygalacturonic acid chain whichare the 132 normal vibration modes of Ac and the 141 normal vibration modes of Es, combining the normal internal coordinates with the SQM methodology. In addition, three subunits were also studied.Reasonable correlations between the experimental and theoretical spectra were obtained. Thus, this work","palabras_clave":"Pectin|Vibrational Spectra|Molecular Structure|DTF calculations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4249,"idioma_id":4,"titulo":"Measurement of shrinkage during photopolymerization of methacrylate resins by interferometric techniques: Local and global analyses","resumen":"Light based measurement techniques have largely proven its efficiency on polymer materials. In thisstudy we describe a method based on specific optical techniques for characterization of dental composites.First, a Dynamic Speckle Pattern (DSP) global analysis was made, which allowed qualitativedescriptions of the behavior of samples during polymerization from their upper surface view. Thisanalysis is complemented with local measurements of shrinkage by means of a fiber optic FizeauInterferometer. Second, a Time History Speckle Pattern (THSP) analysis of different samples was carriedout in order to gain a better understanding of the shrinkage development as the polymerization reactiontakes place. To show the virtues of these techniques, we applied them to both a commercial resin dentalcomposite and a model resin based on methacrylate monomers. Both techniques could serve as a goodcomparing test protocol among different commercial resins.","palabras_clave":"Photopolymerization shrinkage|Fiber optics sensors - Interferometry|Dynamical speckle analysis|Dental composites"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4250,"idioma_id":4,"titulo":"Self-Healing during Electrical Treeing: A Feature of the Two-Phase Liquid-Solid Nature of Silicone Gels","resumen":"We report the results of electrical treeing tests carried out on a range of silicone dielectric gel samples made by varying the ratio of the same two components used for cross-linking a commercial silicone gel. These samples range from liquids to elastomers via two-phase gels. The extent of the curing is followed by means of FTIR spectroscopy and the samples are characterized through their dynamical mechanical properties. It is shown that the gel samples exhibit treeing behavior that includes features typical of both liquids and solids. A filamentary structure is produced that has permanence together with attached bubbles that self-heal locally during treeing, although interesting differences were found depending on the degree of curing. Removal of the treeing voltage allows a more substantial self-healing to occur. This behavior is discussed in terms of the two-phase liquid-solid nature of the silicone gels.","palabras_clave":"SILICONES|ELECTRICAL TREES|SELF-HEALING|GELS|LIQUID FRACTION|CROSS-LINKING|PARTIAL DISCHARGES|CAVITIES|SHEAR MODULUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4251,"idioma_id":4,"titulo":"Effect of hydrocolloids on the properties of wheat\/potato starch mixtures","resumen":"The physicochemical, pasting, thermal, viscoelastic, and syneresis properties of nineformulations prepared with wheat starch, potato starch, and hydrocolloids (pectin, HPMC,arabic gum, and konjac glucomannan (KG)) were assessed. The addition of the hydrocolloidsdecreased the water solubility index and water absorption capacity but did not modify theswelling power of starch granules. The starch gelatinization temperature decreased in thepresence of the hydrocolloids, while the enthalpy of gelatinization and gelatinization rangetemperature increased. Pectin and HPMC addition decreased the paste viscosity whereas KGincreased and arabic gum had no effect. On the other hand, the pasting temperature was notmodified by the presence of hydrocolloids. All the blends behaved as solid as the storage moduluswas higher than the viscous modulus within all the frequency range assayed. Viscouscharacteristics were improved with the addition of pectin and HPMC. The presence ofhydrocolloids increased the stability of the gelled starch mixtures to freeze?thaw cycle and nosyneresis was observed in these gels stored at 4°C.","palabras_clave":"Hydrocolloids|Pasting|Potato starch|Wheat starch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4252,"idioma_id":4,"titulo":"Descri pción de un brote atípico de rabia en terneros en un encierre a corral","resumen":"La rabia paresiante o demodina resulta de la transmisión del virus rábico por lamordedura de murciélagos hematófagos. Esta enfermedad zoonóticaes endémicaen una vasta región del norte argentino.La infección por el virus rábico produce una encefalitis de curso invariablemente mortal. El periodo de incubación esrelativamente largo, y el traslado de animales incubando la infección desde áreas endémicas es un riesgo potencial para la difusión de la enfermedad,con posible impacto sobre la salud pública.Este trabajoinforma un brote de rabia en terneros de un sistema de engorde a corral, al cual fueron introducidosincubando la infección, para luego desarrollar la enfermedad. Se describenlas características clínicas y epidemiológicas del brote y las lesiones macro- y microscópicas observadas. El brote se confirmó mediante la técnica de inmunofluorescencia directa y la inoculación intracraneal de ratones lactantes. La rabia representa un problema sanitario vigente quedebe ser considerado en el diagnóstico diferencial cuando se registran signos nerviosos en animales provenientes de áreas endémicas.","palabras_clave":"Rabia|encefalitis|engorde a corral|enfermedades nerviosas|salud pública"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4253,"idioma_id":4,"titulo":"Assessment of climate variability and land use effect on shallow lakes in temperate plains of Argentina","resumen":"Areal variation in lakes is closely associated with other factors such as intensity of land use of the hydrological basins, climatology, geomorphology and depth. The main objective of this study is to assess the relationship between climatic variability events (El Niño Southern Oscillation, ENSO), land use and shallow lakes areal variations in the temperate plains of Argentina for the period 2002?2011. The standard precipitation evaporation index (SPEI) was employed in this study in conjunction with the oceanic Nin?o index (ONI) and in situ annual precipitation data to determine wet, dry and normal years.Shallow lakes? areal variation was analyzed by satellite imagery (MODIS) processing and the variability of the land use intensity was determined based on agricultural estimations. Frequency in the maximum of shallow lake areas was relatively more dependent on any changes in the rainfall regime (ENSO events) than in the land use (decrease\/increase of the cultivated area). The El Niño (2002\/3) and La Niña (2007\/8) events clearly affected the precipitationsof the total studied lake regions, with maximum areas in 2002 and minimum in 2008. The results obtained may be an important contribution to mitigate flooding and droughts in the studied area.","palabras_clave":"SHALLOW LAKES|TEMPERATE PAMPA REGION|ENSO EVENTS|AREAL VARIABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4254,"idioma_id":4,"titulo":"A combined DPSIR and SAF approach for the adaptive management of beach erosion in Monte Hermoso and Pehuen Co (Argentina)","resumen":"A combined DPSIR and SAF approach for the adaptive management of beach erosion in Monte Hermoso and Pehuen Co (Argentina)Vera Semeoshenkova, Alice Newton, Mara Rojas, M. Cintia Piccolo, M. Lujan Bustos, M. Andrea Huamantinco Cisneros and Leonardo G. BerninsoneAbstractCoastal areas experience multiple pressures from anthropogenic activities that negatively change the ecological and environmental status of beaches and impact human welfare. The focus of this paper is coastal erosion, an issue that is very relevant for Argentina, the second largest nation in Latin America with an extensive coastline of nearly 5000 km. Coastal erosion decreases the attractiveness of coastal areas for tourism development, leading to considerable economic impacts. In this paper, two complementary approaches, the Driver-Pressure-State-Impact-Response (DPSIR) and the Systems Approach Framework (SAF), were used to analyse two beach resorts in Argentina: Monte Hermoso and Pehuen Co.Application of the SAF included stakeholder mapping, governance (institutional) mapping and issue identification. During the participatory meetings with stakeholders and decision makers, the problem of coastal erosion was identified as the most important in the region. The joint approach of DPSIR and SAF contributed to: (i) the determination and description of the economic drivers of coastal erosion; (ii) the identification of the associated activities and pressures; (iii) the assessment of the ecological and environmental state of coastal areas; (iv) the assessment of impacts of environmental changes on human wellbeing; and finally (v) a proposal of the possible management responses for mitigating the coastal erosion problem and the sustainable development of the region to the responsible authorities.","palabras_clave":"COASTAL EROSION|BEACH MANAGEMENT|DPSIR|SAF"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4255,"idioma_id":5,"titulo":"Evolucion de los roedores Cricetidae en América del Sur: una visión desde el registro paleontológico","resumen":"La historia evolutiva de la subfamilia Sigmodontidae, el segundo grupo más especioso de mamíferos vivientes, es discutida desde el punto de vista paleontológico. En contraste con los hallazgos importantes provenientes de los datos moleculares, los pocos fósiles conocidos parecen proveer un escenario más pobre. Sin embargo, ellos puntualizan varios hechos y problemas no resueltos, algunos de los cuales son discutidos aquí, incluyendo: (1) una rica asociación norteamericana de taxones Mio-Pliocenos cuya afiliación a Sigmodontinae u otro grupo de cricétidos es controversial; (2) la edad y diversificación de los muroideos sudamericanos más antiguos es todavía discutible; aunque este ha sido tradicionalmente asumido como Plioceno Temprano, puede ser más joven; (3) los fósiles sugieren dos episodios principales de radiación dentro de Sigmodontinae: un pulso antiguo durante el Plioceno, caracterizado por géneros extintos y una segunda oleada (al menos desde el Pleistoceno Medio) incluyendo formas vivientes. Claramente el registro paleontológico de sigmodontinos es un campo promisorio que necesita de nuevos esfuerzos y mentes jóvenes.","palabras_clave":"SIGMODONTINAE|AMERICA DEL SUR|PLIOCENO|PLEISTOCENO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4256,"idioma_id":4,"titulo":"Theme in English and Spanish: Different means of realization for the same textual function","resumen":"This article offers a comparative view of a Systemic Functional (SF) account of Theme in English and Spanish declarative clauses. It considers the lexicogrammatical realization of Theme in both languages and shows how Themes across the clauses construe the method of development of a phase of discourse in the respective languages, unmarked Themes scaffolding textual continuity and marked Themes scaffolding transitions between discourse phases. The paper reviews the concept of Theme in Systemic Functional Linguistics (SFL) and analyzes texts in both languages, taking into account a trinocular perspective (Matthiessen & Halliday 2009): from above, considering textual discourse functions related to the lexicogrammatical function of Theme; from roundabout, considering other textual functions in the clause as New; and from below, paying attention to the role that different ranks may play in the realization of the function under focus. In addition, the paper argues for a re-interpretation of previous SFL accounts of Spanish Theme, on the assumptions of SFL language typology. Based on the analysis of fragments of Research Articles, the paper shows how English and Spanish texts perform similar strategies to maintain the method of development of a text, through the interaction between lexical strings and reference chains with the function of Theme (Fries 1981; Martin 1992). It shows, however, that the lexicogrammatical realization of unmarked Theme differs between these languages.","palabras_clave":"ACADEMIC DISCOURSE|ENGLISH|METHOD OF DEVELOPMENT|SPANISH|THEME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4257,"idioma_id":5,"titulo":"Esquema de tres interfaces con compensación isostática local en el margen continental Argentino","resumen":"In the present paper, we investigate the Airy type isostatic compensation scheme for three interfaces:water-sediments, sediments-crust and crust-mantle, using forward modelling to calculate the isostaticanomaly with different approximations of Parker?s expression in the Argentine continentalmargin. The isostatic anomaly is estimated as the residue between the observed free air anomaly andthe calculated free air anomaly through the sum of the contributions from the tree interfaces.After that, we perform the inversion of the isostatic anomaly in order to find the deflection of theMoho necessary to compensate it (or minimize it) using one term of Parker?s expression in the inversion, as an experiment to test the numerical methods. The Moho here considered is obtained fromknowing the depth of the basement and crustal thickness. Another way to estimate the crust-mantleinterface (Moho) is by the inversion with the iterative method of Parker-Oldenburg (Parker, 1973;Oldenburg, 1974), which allows inverting the Bouguer anomaly with more than one term. With theMoho thus obtained, a free-air gravity anomaly can be calculated, which reproduces the observedanomaly quite well. Even considering that the experiment constitutes a schematic model just to testthe numerical methods involved, from the comparison with the only available digitized refractionprofile in the study area, we find that the large wavelength of the inverted Moho is reproduced fairlywell by the modeled Moho for both methods. However, the iterative method yields smoother surfaces,possibly more realistic surfaces given the roughness that the other estimates present.","palabras_clave":"compensación isostática local|margen continental Argentino"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4258,"idioma_id":4,"titulo":"Drug-Induced QTc Interval Prolongation: A Multicenter Study to Detect Drugs and Clinical Factors Involved in Every Day Practice.","resumen":"AbstractOBJECTIVE: The actual prevalence of drug induced QTc prolongation in clinical practice is unknown. Our objective was to determine the occurrence and characteristics of drug-induced QT prolongation in several common clinical practices. Additionally, a subgroup of patients treated with dextropropoxyphene of particular interest for the regulatory authority was analysed. RESEARCH DESIGN AND METHODS: Medical history and comorbidities predisposing to QT interval prolongation were registered for 1270 patient requiring medical assistance that involved drug administration. Three ionograms and ECGs were performed: baseline, intra- and after treatment; QT interval was corrected with Bazzet formula. RESULTS: Among patients, 9.9% presented QTc >450\/470 ms, 3% QTc > 500 ms, 12.7% &#916;QTc >30 ms and 5.2% &#916;QTc >60 ms. QTc prolongation associated with congestive heart failure, ischemic cardiopathy, diabetes, renal failure, arrhythmias, hypothyroidism, and bradycardia. At univariate analysis, clarithromycin, haloperidol, tramadol, amiodarone, glyceryl trinitrate, amoxicillin + clavulanic acid, amoxicillin + sulbactam, ampicillin + sulbactam, fentanyl, piperacillin + tazobactam, and diazepam prolonged QTc. Prolongation remained significantly associated with furosemide, clarithromycin, glyceryl trinitrate and betalactamase inhibitors after multivariate analysis. CONCLUSION:QT interval prolongation in everyday practice is frequent, in association to clinical factors and drugs that can be easily identified for monitoring and prevention strategies.","palabras_clave":"Farmacovigilancia|QT|Arritmia|Factores de riesgo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4259,"idioma_id":4,"titulo":"Anti-tubercular and antioxidant activities of C-glycosyl carbonic anhydrase inhibitors: towards the development of novel chemotherapeutic agents against Mycobacterium tuberculosis","resumen":"During the treatment of tuberculosis infection, oxidative stress may result in tissue inflammation due to anti-tubercular drugs. It was suggested that treatment with antioxidant drugs could be beneficial as an adjunct to anti-tuberculosis drug therapy. Recently our group has shown that several C-glycosides are inhibitors of Mycobacterium tuberculosis b-carbonic anhydrases (CAs, EC 4.2.1.1). In an effort to develop novel chemotherapeutic agents against tuberculosis, the anti-tubercular and antioxidant activities of a series of C-glycosides containing the phenol or the methoxyaryl moiety were studied. Many compounds showed inhibition of growth of  M. tuberculosis H37Rv strain and good antioxidant ability. A glycomimetic incorporating the 3-hydroxyphenyl moiety showed the best activity profile, and therefore this functionality represents lead for the development of novel anti-tubercular agents with dual mechanisms of action.","palabras_clave":"Tuberculosis|Glycomimetic|Dual mechanisms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4260,"idioma_id":5,"titulo":"Problematizando las enseñanzas sobre la menstruación en la escuela: lo disimulado, lo negativo, lo silenciado","resumen":"El propósito de este trabajo es problematizar las enseñanzas sobre la menstruación en la escuela. A partir de una revisión de bibliografía especializada, presentamos los significados atribuidos a la menstruación en nuestra sociedad y en las publicidades. Esta caracterización nos lleva a preguntarnos si podemos dejar de lado las significaciones culturales en las clases de biología. De manera más general, planteamos la necesidad de repensar las enseñanzas sobre la corporalidad como forma de hacer educación sexual.","palabras_clave":"Menstruación|educación sexual|corporalidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4261,"idioma_id":4,"titulo":"mRNA biogenesis-related helicase eIF4AIII from Arabidopsis  thaliana is an important factor for abiotic stress adaptation","resumen":"Similar to other plant species, Arabidopsis has a huge repertoire of predicted helicases, including the eIF4AIII factor, a putative component of the exon junction complex related to mRNA biogenesis. In this article, we integrated evolutionary and functional approaches to have a better understanding of eIF4AIII function in plants. Phylogenetic analysis showed that the mRNA biogenesis-related helicase eIF4AIII is the ortholog of the stress-related helicases PDH45 from Pisum sativum and MH1 from Medicago sativa, suggesting evolutionary and probably functional equivalences between mRNA biogenesis and stress-related plant helicases. Molecular and genetic analyses confirmed the relevance of eIF4AIII during abiotic stress adaptation in Arabidopsis. Therefore, in addition to its function in mRNA biogenesis, eIF4AIII can play a role in abiotic stress adaptation","palabras_clave":"ABIOTIC STRESS|mRNA BIOGENESIS|EVOLUTION|HELICASAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4262,"idioma_id":4,"titulo":"Application of Model predictive control suitable for closed loop re-identification to a polymerization reactor","resumen":"Recently, a linear Model Predictive Control (MPC) suitable for closed-loopre-identication was proposed, which solves the potential conflict between thepersistent excitation of the system (necessary to perform a suitable identi-cation) and the control, and guarantees recursive feasibility and attractivityof an invariant region of the closed-loop. This approach, however, needs tobe extended to account for a proper robustness to moderate-to-severe modelmismatches, given that re-identications are necessary when the system is notclose to the operating point where the current linear model was identied. Inthis work, new results on robustness are presented, and an exhaustive appli-cation of the new MPC suitable for closed-loop re-identication to a nonlinearpolymerization reactor simulator is made to explore the diculties arising froma real life identication. Furthermore, several closed-loop re-identications areperformed in order to clearly show that the proposed controller provides un-correlated input-output data sets, which together with the guaranteed stability,constitute the main controller benefit.","palabras_clave":"Model predictive control|Closed-loop identification|Polymerization reactor|Persistent excitation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4263,"idioma_id":4,"titulo":"Species-Specific Responses of Carnivores to Human-Induced Landscape Changes in Central Argentina","resumen":"The role that mammalian carnivores play in ecosystems can be deeply altered by humandriven habitat disturbance. While most carnivore species are negatively affected, the impact of habitat changes is expected to depend on their ecological flexibility. We aimed to identify key factors affecting the habitat use by four sympatric carnivore species in landscapes of central Argentina. Camera trapping surveys were carried out at 49 sites from 2011 to 2013. Each site was characterized by 12 habitat attributes, including human disturbance and fragmentation. Four landscape gradients were created from Principal Component Analysis and their influence on species-specific habitat use was studied using Generalized Linear Models. We recorded 74 events of Conepatus chinga, 546 of Pseudalopex gymnocercus, 193 of Leopardus geoffroyi and 45 of Puma concolor. We found that the gradient describing sites away from urban settlements and with low levels of disturbance had the strongest influence. L. geoffroyi was the only species responding significantly to the four gradients and showing a positive response to modified habitats, which could be favored by the low level of persecution by humans. P. concolor made stronger use of most preserved sites with low proportion of cropland, even though the species also used sites with an intermediate level of fragmentation. A more flexible use of space was found for C. chinga and P. gymnocercus. Our results demonstrate that the impact of human activities spans across this guild of carnivores and that species-specific responses appear to be mediated by ecological and behavioral attributes.","palabras_clave":"CARNIVORES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4264,"idioma_id":4,"titulo":"Cultivable bacteria associated with infective propagules of arbuscular mycorrhizal fungi. Implications for mycorrhizal activity","resumen":"This study aimed to isolate and characterize bacteria associated with surface-sterilized germinated propagules of arbuscular mycorrhizal (AM) fungi. It also aimed to evaluate their activity as mycorrhization helper bacteria (MHB) on the AM fungus Rhizophagus intraradices, which is commonly used in the formulation of bioinoculants. Most isolated bacteria did not significantly affect the viability and subsequent growth of mycelia. Azospirillum sp., Rhizobium etli, Bacillus megaterium,Bacillus sp., and Paenibacillus rhizosphaerae significantly enhanced pre-symbiotic variables (the re-growth\/germination and the mycelia formed from AM propagules). P. rhizosphaerae, Azospirillum sp., and R. etli also increased extraradical mycelial length, mycorrhization percentages and the number of newly formed spores. The isolated MHB were characterized based on their starch-degrading ability, indole acetic acid production, phosphate solubilization, and inhibition of phytopathogenic fungal growth. Results suggest that some of the MHB studied, in association with viable AM propagules, could be potentially used as complex microbial inoculants for plant growth promotion. ","palabras_clave":"ARBUSCULAR MYCORRHIZAL FUNGI|ASSOCIATED HELPER RHIZOBACTERIA|COMPLEX MICROBIAL INOCULANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4265,"idioma_id":4,"titulo":"Innate host habitat preference in the parasitoid Diachasmimorpha longicaudata: Functional significance and modifications through learning","resumen":"Parasitoids searching for polyphagous herbivores can find their hosts in a variety of habitats. Under this scenario, chemical cues from the host habitat (not related to the host) represent poor indicators of host location. Hence, it is unlikely that naïve females show a strong response to host habitat cues, which would become important only if the parasitoids learn to associate such cues to the host presence. This concept does not consider that habitats can vary in profitability or host nutritional quality, which according to the optimal foraging theory and the preference-performance hypothesis (respectively) could shape the way in which parasitoids make use of chemical cues from the host habitat. We assessed innate preference in the fruit fly parasitoid Diachasmimorpha longicaudata among chemical cues from four host habitats (apple, fig, orange and peach) using a Y-tube olfactometer. Contrary to what was predicted, we found a hierarchic pattern of preference. The parasitism rate realized on these fruit species and the weight of the host correlates positively, to some extent, with the preference pattern, whereas preference did not correlate with survival and fecundity of the progeny. As expected for a parasitoid foraging for generalist hosts, habitat preference changed markedly depending on their previous experience and the abundance of hosts. These findings suggest that the pattern of preference for host habitats is attributable to differences in encounter rate and host quality. Host habitat preference seems to be, however, quite plastic and easily modified according to the information obtained during foraging.","palabras_clave":"a"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4266,"idioma_id":4,"titulo":"From Farm to Table: Follow-Up of Shiga Toxin-Producing Escherichia coli Throughout the Pork Production Chain in Argentina","resumen":"Pigs are important reservoirs of Shiga toxin-producing Escherichia coli (STEC). Theentrance of these strains into the food chain implies a risk to consumers becauseof the severity of hemolytic uremic syndrome. This study reports the prevalence andcharacterization of STEC throughout the pork production chain. From 764 samples,31 (4.05%) were stx positive by PCR screening. At farms, 2.86% of samples were stxpositive; at slaughter, 4.08% of carcasses were stx positive and at boning rooms, 6%of samples were stx positive. These percentages decreased in pork meat ready for sale at sales markets (4.59%). From positive samples, 50 isolates could be characterized. At farms 37.5% of the isolates carried stx1\/stx2 genes, 37.5% possessed stx2e and 25%, carried only stx2. At slaughter we detected 50% of isolates positive for stx2, 33% for stx2e, and 16% for stx1\/stx2. At boning rooms 59% of the isolates carried stx1\/stx2, 14% stx2e, and 5% stx1\/stx2\/stx2e. At retail markets 66% of isolates were positive for stx2, 17% stx2e, and 17% stx1\/stx2. For the other virulence factors, ehxA and saa were not detected and eae gene was detected in 12% of the isolates. Concerning putative adhesins, agn43 was detected in 72%, ehaA in 26%, aida in 8%, and iha in 6% of isolates. The strains were typed into 14 E. coli O groups (O1, O2, O8, O15, O20, O35, O69, O78, O91, O121, O138, O142, O157, O180) and 10 H groups (H9, H10, H16, H21, H26, H29, H30, H32, H45, H46). This study reports the prevalence and characterization of STEC strains through the chain pork suggesting the vertical transmission. STEC contamination originates in the farms and is transferred from pigs to carcasses in the slaughter process and increase in meat pork at boning rooms and sales markets. These results highlight the need to implement an integrated STEC control system based on good management practices on the farm and critical control point systems in the food chain.","palabras_clave":"STEC,|,foodbornepathogens|porkproductionchain|prevalence,|characterization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4267,"idioma_id":4,"titulo":"Mode of Action of the Sesquiterpene Lactones Psilostachyin and Psilostachyin C on Trypanosoma cruzi","resumen":"Trypanosoma cruzi is the causative agent of Chagas? disease, which is a major endemic disease in Latin America and is recognized by the WHO as one of the 17 neglected tropical diseases in the world. Psilostachyin and psilostachyin C, two sesquiterpene lactones isolated from Ambrosia spp., have been demonstrated to have trypanocidal activity. Considering both the potential therapeutic targets present in the parasite, and the several mechanisms of action proposed for sesquiterpene lactones, the aim of this work was to characterize the mode of action of psilostachyin and psilostachyin C on Trypanosoma cruzi and to identify the possible targets for these molecules. Psilostachyin and psilostachyin Cwere isolated from Ambrosia tenuifolia and Ambrosia scabra, respectively. Interaction of sesquiterpene lactones with hemin, the induction of oxidative stress, the inhibition of cruzipain and trypanothione reductase and their ability to inhibit sterol biosynthesis were evaluated.The induction of cell death by apoptosis was also evaluated by analyzingphosphatidylserine exposure detected using annexin-V\/propidium iodide, decreased mitochondrial membrane potential, assessed with Rhodamine 123 and nuclear DNA fragmentation evaluated by the TUNEL assay. Both STLs were capable of interacting with hemin.Psilostachyin increased about 5 times the generation of reactive oxygen species in Trypanosoma cruzi after a 4h treatment, unlike psilostachyin C which induced an increase in reactive oxygen species levels of only 1.5 times. Only psilostachyin C was able to inhibit the biosynthesis of ergosterol, causing an accumulation of squalene. Both sesquiterpene lactones induced parasite death by apoptosis. Upon evaluating the combination of both compounds,and additive trypanocidal effect was observed. Despite their structural similarity, both sesquiterpene lactones exerted their anti-T. cruzi activity through interaction with different targets. Psilostachyin accomplished its antiparasitic effect by interacting with hemin, while psilostachyin C interfered with sterol synthesis.","palabras_clave":"TRYPANOSOMA CRUZI|SESQUITERPENE LACTONES|CHAGAS' DISEASE|HEMIN INTERACTION|OXIDATIVE DAMAGE|ACTION MECHANISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4268,"idioma_id":4,"titulo":"Dynamics of expression of two vitellogenin genes in the Chagas disease  vector   Triatoma  infestans: Analysis throughout pre-vitellogenesis  and vitellogenesis","resumen":"The reproductive success of all oviparous species depends on vitellogenin (Vg) biosynthesis and its accumulation in the developing oocytes. The expression levels of two Vg genes (Vg1 and Vg2) were analyzed by qPCR and western blot in fat body and ovaries of adult females, at different times after ecdysis (pre-vitellogenic phase) and after blood feeding of females (vitellogenic phase). Vg genes were also evaluated in fat bodies of adult males as well as in female fifth instar nymphs. No trace of Vg mRNA was detected in adult males or in nymphs. Vg1 and Vg2 were expressed in the fat bodies and ovaries of adult females. The Vg genes start to be expressed slightly in both tissues of adult females during pre-vitellogenesis. After blood feeding, Vg1 and Vg2 were up regulated and significant levels of Vg transcripts as well as protein expression were observed in fat bodies sampled throughout vitellogenesis. During this period however, the distribution patterns of Vg1 and Vg2 transcripts showed two peaks around early and advanced vitellogenesis (days 4 and 12 post-feeding, respectively). In the ovaries, levels of mRNAs increased from the day 10 post-blood feeding onwards. In addition, the immunofluorescence assays showed a strong signal for vitellin in the yolk bodies of terminal follicles of vitellogenic females. The involvement of fat body and ovary in the synthesis of Vg suggests different roles of Vgs in supporting the growth of oocytes.","palabras_clave":"TRIATOMA INFESTANS|CHAGAS´ DISEASE|VITELLOGENIN|EXPRESSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4269,"idioma_id":4,"titulo":"Acute exposure to Buenos Aires air particles (UAP-BA) induces local and systemic inflammatory response in middle-aged mice: A time course study","resumen":"Exposure to air particulate matter (PM) is associated with increased cardiovascular morbimortality. However, PM doesn't affect equally to all people, being the old cohort the most susceptible and studied. We hypothesized that another specific life phase, the middle-aged subpopulation, may be negatively affected. Therefore, the aim of this study was to analyze in vivo the acute biological impact of two environmental particles, Urban Air Particles from Buenos Aires and Residual Oil Fly Ash, on the cardiorespiratory system of middle-aged mice, evaluating oxidative metabolism and inflammation. Both PM provoked a local and systemic inflammatory response, leading to a reduced alveolar area in the lung, an epicard inflammation in the heart, an increment of IL-6, and a reduction on PON 1 activity in serum of middle-aged animals. The positive correlation of local parameters with systemic markers of oxidative stress and inflammation could be responsible for associations of cardiovascular morbimortality in this subpopulation.","palabras_clave":"URBAN AIR POLLUTION|CARDIORESPIRATORY SYSTEM|INFLAMMATION|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4271,"idioma_id":5,"titulo":"Experiencias de calorimetría diferencial de barrido en aleaciones Al-Si","resumen":"La observación de agujas características de una transformación displaciva en aleaciones U-Al-Si en el entorno del compuesto U3Si5 evidencia la necesidad de analizar las distintas transformaciones que ocurren durante el enfriamientoutilizando una técnica dinámica de identificación de transformaciones (calorimetría diferencial de barrido).Paraevaluar la versatilidad de esta técnica, se estudiaron aleaciones Al-Si con concentraciones de Si entre 0,6% y 7,1% en peso. Se determinaron las temperaturas liquidus y eutéctica de cada aleación y se estimó la entalpía de formación de los precipitados de Si y de la reacción eutéctica. Los resultados reproducen las temperaturas de ambas reaccionessegún el diagrama de equilibrio del sistema binario Al-Si.Para el cálculo de las entalpías de transformación resulta indispensable obtener el porcentaje de fase transformada por otras técnicas de caracterización una vez finalizado el ensayo.","palabras_clave":"liquidus|tranformaciones de fases|Entalpías|caracterización"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4272,"idioma_id":4,"titulo":"The thyroid status reprograms T cell lymphoma growth and modulates immune cell frequencies","resumen":"Abstract: In spite of considerable evidence on the regulation of immunity by thyroid hormones, the impact of the thyroid status in tumor immunity is poorly understood. Here, we evaluated the antitumor immune responses evoked in mice with different thyroid status (euthyroid, hyperthyroid, and hypothyroid) that developed solid tumors or metastases after inoculation of syngeneic T lymphoma cells. Hyperthyroid mice showed increased tumor growth along with increased expression of cell cycle regulators compared to hypothyroid and control tumor-bearing mice. However, hypothyroid mice showed a higher frequency of metastases than the other groups. Hyperthyroid mice bearing tumors displayed a lower number of tumor-infiltrating T lymphocytes, lower percentage of functional IFN-&#947;-producing CD8+ T cells, and higher percentage of CD19+ B cells than euthyroid tumor-bearing mice. However, no differences were found in the distribution of lymphocyte subpopulations in tumor-draining lymph nodes (TDLNs) or spleens among different experimental groups. Interestingly, hypothyroid TDLN showed an increased percentage of regulatory T (Treg) cells, while hyperthyroid mice displayed increased number and activity of splenic NK cells, which frequency declined in spleens from hypothyroid mice. Moreover, a decreased number of splenic myeloid-derived suppressor cells (MDSCs) were found in tumor-bearing hyperthyroid mice as compared to hypothyroid or euthyroid mice. Additionally, hyperthyroid mice showed increased cytotoxic activity, which declined in hypothyroid mice. Thus, low levels of intratumoral cytotoxic activity would favor tumor local growth in hyperthyroid mice, while regional and systemic antitumor response may contribute to tumor dissemination in hypothyroid animals. Our results highlight the importance of monitoring the thyroid status in patients with T cell lymphomas. Key messages: T cell lymphoma phenotype is paradoxically influenced by thyroid status.Hyperthyroidism favors tumor growth and hypothyroidism rises tumor dissemination.Thyroid status affects the distribution of immune cell types in the tumor milieu.Thyroid status also modifies the nature of local and systemic immune responses.","palabras_clave":"T cell lymphoma |Apoptosis|Thyroid hormones| Proliferation|Antitumor immune response"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4273,"idioma_id":4,"titulo":"Eruption dynamics of the 22-23 April 2015 Calbuco Volcano (Southern Chile): Analyses of tephra fall deposits","resumen":"After 54 years since its last major eruption in 1961, Calbuco Volcano (Ensenada, Southern Chile) reawakened with few hours of warning on 22 April 2015 at 18:05 local time. The main explosive eruption consisted of two eruption pulses (lasting ~1.5 and 6 h each one) on 22 and 23 April, producing stratospheric (N15 km height) eruption columns. The erupted materials correspond to porphyritic basaltic andesite (~55 wt.% of SiO2). The tephra fall affected mainly the area northeast of the volcano and the finest ash was deposited over Southern Chile and Patagonia Argentina. We studied the tephra fall deposits of both pulses in terms of stratigraphy, distribution, volume, emplacement dynamics and eruption source parameters. Here, we showfield observations that have been made 5?470 km downwind and distinguish five layers (Layers A, B, B1, C and D) representing different stages of the eruption evolution: eruption onset (Layer A; pulse 1), followed by the first paroxysmalevent (Layer B; pulse 1), in some places interbedded by layer B1, tentatively representing the sedimentation of a secondary plume during the end of pulse 1. We recognized a second paroxysm (Layer C; pulse 2) followed by the waning of the eruption (Layer D; pulse 2). The total calculated bulk tephra fall deposit volume is 0.27 ± 0.007 km3 (0.11?0.13 km3 dense rock equivalent), 38% of which was erupted during the first phase and 62% during the second pulse. This eruption was a magnitude 4.45 event (VEI 4 eruption) of subPlinian type. ","palabras_clave":"CALBUCO VOLCANO|ERUPTION CHRONOLOGY|TEPHRA STRATIGRAPHY|SUBPLINIAN ERUPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4274,"idioma_id":4,"titulo":"Forecasting volcanic ash dispersal and coeval resuspension during the April - May 2015 Calbuco eruption","resumen":"Atmospheric dispersion of volcanic ash from explosive eruptions or from subsequent fallout deposit resuspension causes a range of impacts and disruptions on human activities and ecosystems. The April?May 2015 Calbucoeruption in Chile involved eruption and resuspension activities. We overview the chronology, effects, and products resulting from these events, in order to validate an operational forecast strategy for tephra dispersal.The modelling strategy builds on coupling the meteorological Weather Research and Forecasting (WRF\/ARW) model with the FALL3D dispersal model for eruptive and resuspension processes. The eruption modelling considers two distinct particle granulometries, a preliminary first guess distribution used operationally whenno field data was available yet, and a refined distribution based on field measurements. Volcanological inputs were inferred from eruption reports and results from an Argentina?Chilean ash sample data network, which performed in-situ sampling during the eruption. In order to validate the modelling strategy, results were compared with satellite retrievals and ground deposit measurements. Results indicate that the WRF-FALL3D modelling system can provide reasonable forecasts in both eruption and resuspension modes, particularlywhen the adjusted granulometry is considered. The study also highlights the importance of having dedicated datasets of active volcanoes furnishing first-guess model inputs during the early stages of an eruption.","palabras_clave":"VOLCANIC ASH|PARTICLE RESUSPENSION|DISPERSION MODEL|CALBUCO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4275,"idioma_id":5,"titulo":"Desarrollo y madurez de las actividades de un conglomerado de pymes industriales argentinas","resumen":"Las empresas PYMES del sector metalmecánico de la ciudad de Olavarría aportan a su desarrollo económico pero presentan deficiencias a nivel de productividad, competitividad y  en su gestión. Ante esta realidad se hace necesario intensificar el estudio de sus actividades y recursos para mejorar su situación actual. Por tanto, el objetivo es conocer la disponibilidad de recursos tangibles e intangibles y la forma en que los mismos son gestionados en cada una de las actividades empresariales. Para enmarcar el estudio de las actividades y el uso de recursos asociados se recurre a la cadena de valor, complementado con el enfoque basado en recursos. Se recolectan los datos mediante la aplicación de un instrumento, que indaga sobre el uso de recursos relacionados con las actividades primarias y de apoyo. Los recursos tangibles e intangibles son las variables independientes y las variables dependientes son las actividades primarias y de soporte.Luego, se profundiza el diagnóstico a través de un segundo instrumento, que define el nivel de madurez alcanzado en términos de competitividad. Como resultado del diagnóstico y caracterización del estado competitivo de empresas metalmecánicas de Olavarría se obtiene un grado de desarrollo, asociado al uso de los recursos con valores que van desde 49 al 72% para las actividades primarias y desde 55 al 67% para las de soporte. Al evaluar el aprovechamiento de los recursos en la totalidad de las actividades, a través del nivel de madurez, se obtiene un valor promedio asociado al nivel 2, correspondiente al uso ineficiente de recursos. Esto permite concluir que las mismas cuentan con recursos tangibles e intangibles, pero los mismos no son utilizados eficientemente para realizar sus actividades en pos de lograr una ventaja competitiva","palabras_clave":"uso de recursos|cadena de valor|enfoque basado en recursos|PYMES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4276,"idioma_id":4,"titulo":"Diversity and life-history traits of wild bees in intensive agricultural landscapes in the Rolling Pampa, Argentina","resumen":"The decline of bees is a major concern due to their vital role inpollinating many crops and wild plants. Some regions in SouthAmerica, and especially the Pampas, are amongst those parts ofthe world where stressors of bee populations have been littlestudied. The Pampas has been intensively transformed for agriculture,being presently one of the most productive areas of agriculturalcommodities in the world. Here, we aim to provide firstinsights on the taxonomic and functional composition of beeassemblages in the Rolling Pampa, the most intensively managedpart of the Pampas. Soybean (herbicide-tolerant genetically modifiedvarieties) is the predominant crop in this region. Bees weresampled with coloured pan traps. Sampling points were locatedon field margins in either the cropped or the semi-natural grasslandarea of a farmland site devoted to annual cropping. A total of2384 individuals were caught, representing 33 taxa [mainly (morpho)species]. The subgenus Lasioglossum (Dialictus) largely dominatedcaptures (78% of the total abundance) and was relativelyabundant in the entire study area, suggesting that some speciesare likely to reach their ecological requirements in cropped areas.No-till fields and field margins may provide large areas for thesebelow-ground nesting species, while their polylectic food preferencesallow them to collect pollen on a wide range of plantspecies, among them possibly soybean. On the contrary, the richnessand the abundance of other taxa were higher in the seminaturalarea than in the cropped area. Among them, abovegroundnesting or oil-collecting species, which have more specialisednesting and floral requirements, were highly associated withthe semi-natural area. Our findings highlight the large dominanceof L. (Dialictus) species in this highly intensively managed landscape,and the urgent need of preserving semi-natural habitats tomaintain species-rich and functionally diverse bee communities inthe Pampas","palabras_clave":"functional diversity|herbicide-tolerant genetically modified soybean|Lasioglossum (Dialictus); no-tillage; wild bees|wild bees"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4277,"idioma_id":4,"titulo":"Structural complexity of arthropod guilds is affected by the agricultural landscape heterogeneity generated by fencerows","resumen":"Intensive farming imposes harsh conditions impeding the persistence of most arthropod species within crop &#64257;elds. Hence, arthropods surviving the unfavourable conditions prevailing within crop &#64257;elds may disperse towards nearby uncropped margins, such as fencerows. Here, we evaluate the in&#64258;uence of landscape heterogeneity on the abundance of different guilds, particularly herbivores and their natural enemies. Said heterogeneity mostly derives from fencerow network density. Hence, we developed an approach based on &#64257;tting linear-mixed models to elucidate the effects of landscape heterogeneity and &#64257;eld position (fencerows and crop interiors) on arthropod diversity. Mixed models were &#64257;tted to arthropod data obtained by pitfall trap samplings in 36 crop &#64257;elds. Arthropod communities were structurally and functionally more complex along fencerows than within nearby crop interiors. Arthropods abundance was modulated by landscape heterogeneity, increasing the abundance of natural enemies as the landscape heterogeneity increased. On the contrary, herbivores abundance decreased as landscape heterogeneity increased. Consequently, the ratio between herbivores and natural enemies also decreased as landscapes became more heterogeneous. Natural enemies with larger body sizes, mostly carabid beetles, were more sensitive to landscape homogenisation.Ourstudyrevealsthat,despitethecoarse-grainedlandscapesintheRolling Pampa,fencerowdensityappearsasakeyfactorforstructuringcomplexarthropod guildsin intensivelyfarmed agriculturalmosaics.In landscapeswith higher density of fencerows, arthropods tend to concentrate along them, thus increasing the community structural complexity as well as the predation pressure over herbivores. This structural complexity of upper trophic levels enhances the ?top-down? regulation of herbivore populations, consequently decreasing the probability of pest outbreaks within crop &#64257;elds.","palabras_clave":"field margin|carabid beetles|natural enemies|trophic interactions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4278,"idioma_id":6,"titulo":"Les nouvelles logiques migratoires en Amérique du Sud","resumen":"Les processus migratoires en Amérique du Sud connaissent d?importants changements.Les flux migratoires à l?intérieur du sous-continent augmentent au contraire des destinations traditionnelles comme des États-Unis, le Canada ou l?Europe. Et les États de l?Amérique du Sud souhaitent faciliter la libre circulation de leurs habitants avec un projet de citoyenneté sud-américaine.","palabras_clave":"Procesos migratorios urbanos|Procesos de integración\/segregación|Globalización migratoria y ciudadanía sudamericana |Políticas públicas migratorias"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4279,"idioma_id":4,"titulo":"Pulmonary toxoplasmosis in human immunodeficiency virus-infected patients in the era of antiretroviral therapy","resumen":"Toxoplasmosis is a severe opportunistic infection in patients infected with the human immunodeficiency virus (HIV). The lung is a major site of infection after the central nervous system. In this report we described two cases of pneumonia due to Toxoplasma gondii infection in HIV patients with antiretroviral therapy. Clinical and radiological abnormalities are not specific. Pulmonary toxoplasmosis should be considered in HIV infected patients with late stage of HIV, CD4 count less than 100 cells\/µl and a poor adherence to HAART.","palabras_clave":"PULMONARY TOXOPLASMOSIS|HIV|AIDS|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4280,"idioma_id":4,"titulo":"First report of Cystoisospora belli parasitemia in a patient with acquired immunodeficiency syndrome","resumen":"Cystoisospora belli in patients with the acquired immunodeficiency syndrome (AIDS) has been described as cause of chronic diarrhea and disseminated cystoisosporosis. Diagnosis of intestinal cystoisosporosis can be achieved at the tissue level in the villus epithelium of the small bowel. Disseminated cystoisosporosis is diagnosed by microscopy identification of unizoite tissue cysts in the lamina propria of the intestine. We report a case of disseminated cystoisosporosis in a human immunodeficiency virus (HIV)-infected patient with detection of parasitemia. We studied a 39-year old patient with AIDS and chronic diarrhea by analysis of stool and duodenal biopsy samples. Blood samples were also collected and examined by light microscopy and molecular techniques for C. belli DNA detection. The unizoite tissue cyst stages were present in the lamina propria, with unsporulated oocysts in feces. Zoites were present in blood smears and DNA of C. belli was detected in blood samples. Our study identified a new stage in the life cycle of C. belli. Detection of parasitemia is a novel and noninvasive tool for diagnosis of disseminated cystoisosporosis.","palabras_clave":"CYSTOISOSPORA BELLI|PARASITEMIA|AIDS|UNIZOITE TISSUE CYSTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4281,"idioma_id":4,"titulo":"Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina","resumen":"The identification and characterization of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity ofCryptosporidium in samples obtained fromhospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analyzed by microscopy and TaqMan PCR assays for Cryptosporidium detection, genotyped by nested-PCR-RFLP analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp. were detectedin 26by microscopy\/TaqMan PCR. In samplesfrom Buenos Aires, Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqManPCR and the nested-PCR-RFLP detected C. parvum, C. hominis and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3wereobserved.The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4 and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries.Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.","palabras_clave":"CRYPTOSPORIDIUM|GENOTYPES|GP60|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4282,"idioma_id":4,"titulo":"INTEGRATING PACKAGING AND DISTRIBUTION PROBLEMS AND OPTIMIZATION THROUGH MATHEMATICAL PROGRAMMING","resumen":"This paper analyzes the integration of two combinatorial problems that frequently arise in production and distribution systems. One is the Bin Packing Problem (BPP) problem, which involves finding an ordering of some objects of different volumes to be packed into the minimal number of containers of the same or different size. An optimal solution to this NP-Hard problem can be approximated by means of meta-heuristic methods. On the other hand, we consider the Capacitated Vehicle Routing Problem with Time Windows (CVRPTW), which is a variant of the Travelling Salesman Problem (again a NP-Hard problem) with extra constraints. Here we model those two problems in a single framework and use an evolutionary meta-heuristics to solve them jointly. Furthermore, we use data from a real world company as a test-bed for the method introduced here.","palabras_clave":"BIN PACKING PROBLEM|CAPACITATED VEHICLE ROUTING PROBLEM WITH TIME WINDOWS|LOGISTICS|OPTIMIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4283,"idioma_id":5,"titulo":"Contacto de lenguas y migración: español y guaraní en una villa de Buenos Aires","resumen":"El presente artículo se propone analizar la situación de contacto entre el español y el guaraní en la que se encuentran niños y adultos paraguayos que migraron a la Argentina y residen actualmente en un barrio de la Ciudad de Buenos Aires. Los objetivos particulares del trabajo son estudiar en detalle los sistemas gramaticales de los hablantes, reflexionar sobre el tipo de input que reciben los niños y determinar su incidencia en las producciones infantiles dentro de este contexto de migración. Para esto, presentamos datos correspondientes a un corpus original (Avellana, Brandani y Schmitt en elaboración) de hablantes que residen en una villa de la Ciudad de Buenos Aires (?Villa de los paraguayos?). Se trata de un contexto excepcional para estudiar fenómenos de contacto lingüístico dado que es una comunidad en la que el español paraguayo y el guaraní son los principales vehículos de comunicación.","palabras_clave":"CONTACTO DE LENGUAS |MIGRACIÓN|ESPAÑOL-GUARANÍ|CONTEXTO BILINGÜE Y BIDIALECTAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4284,"idioma_id":4,"titulo":"Natural and induced antibodies contribute to differential susceptibility to secondary cystic echinococcosis of Balb\/c and C57Bl\/6 mice","resumen":"tAntibodies are key immune players in several helminth infections and animal models have been centralfor the identification of their mechanisms of protection. Murine secondary cystic echinococcosis is auseful model for studying Echinococcus granulosus immunobiology, being the immune profile mountedby the experimental host a determinant of parasite success or failure in infection establishment. In thepresent study, we analyzed infection outcome using Balb\/c and C57Bl\/6 mice strains, and compared theirantibody responses in terms of quality and intensity. Our results showed that Balb\/c is a highly susceptiblestrain to secondary cystic echinococcosis, while C57Bl\/6 mice are quite resistant. Moreover, significantdifferences between strains were observed in natural and induced antibodies recognizing E. granulosusantigens, both at the systemic and peritoneal levels. Natural cross-reacting IgM, IgG2b and IgG3 antibodieswere detected in sera from both strains but with different intensities, and ? remarkably ? natural IgG2bshowed to be an intrinsic correlate of protection in both mice strains. Interestingly, naïve C57Bl\/6 serumdisplayed a higher protoscolicidal activity, and heterologous ? but not homologous ? transference ofC57Bl\/6 naïve serum into Balb\/c mice, significantly reduced their infection susceptibility. In the peritonealcavity, different levels of natural cross-reacting IgM and IgG3 antibodies were detected in both micestrains, while cross-reacting IgG2b was detected only in C57Bl\/6 mice. On the other hand, infected micefrom both strains developed isotype-mixed antibody responses, with Balb\/c mice biasing their responsetowards high avidity IgG1 and C57Bl\/6 mice showing a predominance of mixed IgM\/IgG2c\/IgG2b\/IgG3.In this regard, IgG1 levels showed to be a correlate of susceptibility in both mice strains. In conclusion,our results suggest that antibodies ? either natural or induced ? play a role in the susceptibility degreeto murine secondary cystic echinococcosis.","palabras_clave":"SUSCEPTIBILITY|RESISTANCE|NATURAL ANTIBODIES|ECHINOCOCCUS GRANULOSUS|BALB\/C|C57BL\/6"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4285,"idioma_id":4,"titulo":"MicroRNA discovery in the human parasite Echinococcus multilocularis from genome-wide data","resumen":"The cestode parasite Echinococcus multilocularis is the aetiological agent of alveolar echinococcosis, responsible for considerable human morbidity and mortality. This disease is a worldwide zoonosis of major public health concern and is considered a neglected disease by the World Health Organization. The complete genome of E. multilocularis has been recently sequenced and assembled in a collaborative effort between the Wellcome Trust Sanger Institute and our group, with the main aim of analyzing protein-coding genes. These analyses suggested that approximately 10% of E. multilocularis genome is composed of protein-coding regions. This shows there is still a vast proportion of the genome that needs to be explored, including non-coding RNAs such as small RNAs (sRNAs). Within this class of small regulatory RNAs, microRNAs (miRNAs) can be found, which have been identified in many different organisms ranging from viruses to higher eukaryotes. MiRNAs are a key regulation mechanism of gene expression at post-transcriptional level and play important roles in biological processes such as development, proliferation, cell differentiation and metabolism in animals and plants. In spite of this, identification of miRNAs directly from genome-wide data only is still a very challenging task. There are many miRNAs that remain unidentified due to the lack of either sequence information of particular phylums or appropriate algorithms to identify novel miRNAs. The motivation for this work is the discovery of new miRNAs in E. multilocularis based on non-target genomic data only, in order to obtain useful information from the currently available unexplored data. In this work, we present the discovery of new pre-miRNAs in the E. multilocularis genome through a novel approach based on machine learning. We have extracted the most commonly used structural features from the folded sequences of the parasite genome: triplets, minimum free energy and sequence length. These features have been used to train a novel deep architecture of self-organizing maps (SOMs). This model can be trained with a high class imbalance and without the artificial definition of a negative class. We discovered 886 pre-miRNA candidates within the E. multilocularis genome-wide data. After that, experimental validation by small RNA-seq analysis clearly showed 23 pre-miRNA candidates with a pattern compatible with miRNA biogenesis, indicating them as high confidence miRNAs. We discovered new pre-miRNA candidates in E. multilocularis using non-target genomic data only. Predictions were meaningful using only sequence data, with no need of RNA-seq data or target analysis for prediction. Furthermore, the methodology employed can be easily adapted and applied on any draft genomes, which are actually the most interesting ones since most non-model organisms have this kind of status and carry real biological and sanitary relevance.","palabras_clave":"MACHINE LEARNING|SELF ORGANIZING MAP|MICRORNA|ECHINOCOCCUS MULTILOCULARIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4286,"idioma_id":5,"titulo":"MUTACIÓN FUNDADORA EN SÍNDROME DE LYNCH TIPO II","resumen":"El síndrome de Lynch es la más frecuente de las neoplasias colorrectales hereditarias. Se origina por mutaciones germinales deletéreas familio-específicas en los genes que codifican proteínas de reparación del ADN: MLH1 (homólogo humano de mutL), MSH2 y MSH6 (homólogo humano de mutS 2 y 6, respectivamente), PMS2 (homólogo humano de PMS1 2) y MUTYH (homólogo humano de la ADN-glycosilasa mutY). La mutación c.2252_2253delAA, p.Lys751Serfs*3 en el exón 19 del gen MLH1 segrega con un haplotipo descripto en la región norte de Italia y cuyo origen fue atribuido a un efecto fundador. Esta mutación co-segrega con características típicas del síndrome de Lynch, incluyendo afectación temprana y múltiples tumores primarios en el mismo individuo, una alta frecuencia de cáncer pancreático, elevada inestabilidad microsatelital y falta de expresión de PMS2. En el presente trabajo se comunica dicha mutación en una paciente argentina con adenocarcinoma endometroide en cuya historia familiar existen antecedentes de cáncer de colon diagnosticado antes de los 50 años en familiares de primer grado, reuniendo los criterios de Ámsterdam I y síndrome de Lynch II. Los polimorfismos presentes en la paciente coinciden con el haplotipo descripto en una región del norte de Italia. El alto grado de patogenicidad asociada a esta mutación hace imprescindible el estudio de todos los integrantes de las familias con cáncer hereditario permitiendo el diagnóstico genético pre-sintomático, la instauración de tratamientos o conductas preventivas y su seguimiento.","palabras_clave":"Síndrome de Lynch|mutación fundadora| gen MLH1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4287,"idioma_id":4,"titulo":"Cooking methods and the formation of PhIP (2-Amino, 1-methyl, 6-phenylimidazo[4,5-b] pyridine) in the crust of the habitually consumed meat in Argentina","resumen":"Content of carcinogenic molecules like, 2-Amino, 1-methyl, 6-phenylimidazo[4,5-b] pyridine in meals is one of the main mutagenic substances formed during meat cooking, and it can be used as a dietary exposure marker. Our objective was to estimate the amount of PhIP consumed from habitual Argentinean diet, rich in red meats, comparing different cooking procedures and meat type. Samples (n = 240) of lean and fatty beef, chicken, pork, and fish were cooked using different methods: griddle, grill, sauté pan, and oven. Samples were: Overcooked, or cooked with a microbiologically suitable or 'healthy technique' (HT). The PhIP was determined by HPLC-MS. Meats cooked using HT formed little crust amounts and PhIP was below the detection levels. In overcooked meats, large amounts of crust were formed in lean meats, fatty beef, fatty chicken and baked pork. PhIP was measured in lean meats sauted or cooked on a griddle, a method reaching temperatures until 250 °C.It was estimated that Argentine people eats about 12,268.0 ng\/day of PhIP being these values above those tolerated limits for total dietary heterocyclic amines in some developed countries. Hence, cooking small meat portions, at medium temperature, avoiding prolonged cooking and preferring baked lean meats could be recommended as a healthier habit.","palabras_clave":"PhIp|carnes|métodos de cocción"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4288,"idioma_id":4,"titulo":"First Approach to a Framework for Regional Road-Traffic Accidents Reduction System","resumen":"Several conditions as driver imprudence, road conditions and obstacles are the main factors that will cause road accidents. The most important automotive industries are incorporating technology to reduce risk in vehicles. Their products are expensive and lack flexibility to incorporate new features. This work presented a first approach to increase vehicle safety based on regional features. A framework was implemented, incorporating lane analysis and obstacle detection through image processing. The framework was tested using image datasets and real captures with satisfactory results.","palabras_clave":"Driver Assistance|Framework|Automotive Risk Detection"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4289,"idioma_id":4,"titulo":"SOXC and miR17-92 gene expression profiling defines two subgroups with different clinical outcome in mantle cell lymphoma.","resumen":"Mantle cell lymphoma (MCL) is a heterogeneous B-cell lymphoid malignancy where most patients follow an aggressive clinical course whereas others are associated with an indolent performance. SOX4, SOX11, and SOX12 belong to SOXC family of transcription factors involved in embryonic neurogenesis and tissue remodeling. Among them, SOX11 has been found aberrantly expressed in most aggressive MCL patients, being considered a reliable biomarker in the pathology. Several studies have revealed that microRNAs (miRs) from the miR-17-92 cluster are among the most deregulated miRNAs in human cancers, still little is known about this cluster in MCL. In this study we screened the transcriptional profiles of 70 MCL patients for SOXC cluster and miR17, miR18a, miR19b and miR92a, from the miR-17-92 cluster. Gene expression analysis showed higher SOX11 and SOX12 levels compared to SOX4 (P&#8201;&#8804;&#8201;0.0026). Moreover we found a negative correlation between the expression of SOX11 and SOX4 (P&#8201;<&#8201;0.0001). miR17-92 cluster analysis showed that miR19b and miR92a exhibited higher levels than miR17 and miR18a (P&#8201;<&#8201;0.0001). Unsupervised hierarchical clustering revealed two subgroups with significant differences in relation to aggressive MCL features, such as blastoid morphological variant (P&#8201;=&#8201;0.0412), nodal presentation (P&#8201;=&#8201;0.0492), CD5+ (P&#8201;=&#8201;0.0004) and shorter overall survival (P&#8201;<&#8201;0.0001). Together, our findings show for the first time an association between the differential expression profiles of SOXC and miR17-92 clusters in MCL and also relate them to different clinical subtypes of the disease adding new biological information that may contribute to a better understanding of this pathology","palabras_clave":"Mantle cell lymphoma|microRNAs|SOXC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4290,"idioma_id":4,"titulo":"The urinary excretion of an organic anion transporter as an early biomarker of methotrexate-induced kidney injury","resumen":"Methotrexate(MTX) belongs to the group of medicines known as antimetabolites. It iscommonly used in the treatment of malignant diseases and is prescribed in autoimmuneand chronic inflammatory disorders. Along with its effective therapeutic power,MTX has adverse effects on several organs, including the kidney. The organicanion transporter 5 (Oat5) is exclusively localized in the renal apicalmembrane. Oat5 urinary excretion was proposed as an early biomarker in ischemicand nephrotoxic-induced kidney injury and in renal damage due to vascular calcification in preclinical models. Theaim of this study was to evaluate Oat5 renal expression and urinary excretionin rats 48 h after the exposure to different doses of MTX, in comparison withtraditional markers of renal injury, such as creatinine and urea plasma levels,protein urinary levels, urinary alkaline phosphatase (AP) activity, fractional excretion of water (FEWater) and renal histology. Male Wistarrats were treated with a singleintraperitoneal injection of MTX at different dosage: 40-80-120-180-360 mg\/kgb.w. (M40, M80, M120, M180, M360, n = 4, respectively) and experiments werecarried out 48 h after MTX administration. Oat5 renal expression was evaluatedby Western blotting and immunohistochemistry. Traditional parameters were onlymodified at the higher MTX dose (M360). Conversely, Oat5 urinary excretion was elevatedat the middle dose of 80 mg\/kg, b.w. Oat5 renal expression was modified at thehighest dose as well, both in homogenates and in apical membranes. Theseresults suggest that Oat5 urinary excretion might serve as an early biomarkerof MTX-induced kidney injury. ","palabras_clave":"METHOTREXATE|KIDNEY DAMAGE|BIOMARKERS|OAT5"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4291,"idioma_id":4,"titulo":"Comparative prognostic relevance of breast intra-tumoral microvessel density evaluated by CD105 and CD146: A pilot study of 42 cases","resumen":"Angiogenesis is a key process for metastatic progression. While it has been established that the evaluation of breast tumoral microvessel density by CD105 marker is a potential prognostic parameter, its evaluation by CD146 marker has been poorly studied. Aim: The purpose of this study was to compare the prognostic value of intra-tumoral microvessel density assayed by CD105 and CD146 in early breast cancer patients. Methods: 42 women with breast infiltrative ductal carcinoma (I and II-stages) were retrospectively reviewed. Intra-tumoral microvessel density was immunohistochemically examined using antibodies anti-CD105 and CD146 in paraffin-embedded tissues, and their association with classical prognostic-markers, metastatic recurrence, metastasis-free survival and overall survival was analyzed. Results: High microvessel density assessed by CD146 was significantly associated with a higher risk of developing metastasis (p = 0.0310) and a shorter metastasis-free survival (p = 0.0197). In contrast, when we used the CD105-antibody, we did not find any significant association. Finally, CD146 showed to be an independent predictive indicator for metastasis-free survival (p = 0.0055). Conclusion: Our data suggest that the intra-tumoral microvessel density evaluated by CD146 may be a more suitable predictor of metastatic development than that evaluated by CD105 in early breast cancer.","palabras_clave":"breast cancer|metastasis|CD105|CD146"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4292,"idioma_id":4,"titulo":"CRISPR-on system for the activation of the endogenous human INS gene","resumen":"Advances in the field of epigenetics have allowed the design of new therapeutic strategies to address complex diseases such as type 1 diabetes (T1D). Clustered regularly interspaced short palindromic repeats (CRISPR)-on is a novel and powerful RNA-guided transcriptional activator system that can turn on specific gene expression; however, it remains unclear whether this system can be widely used or whether its use will be restricted depending on cell types, methylation promoter statuses or the capacity to modulate chromatin state. Our results revealed that the CRISPR-on system fused with transcriptional activators (dCas9-VP160) activated endogenous human INS, which is a silenced gene with a fully methylated promoter. Similarly, we observed a synergistic effect on gene activation when multiple single guide RNAs were used, and the transcriptional activation was maintained until day 21. Regarding the epigenetic profile, the targeted promoter gene did not exhibit alteration in its methylation status but rather exhibited altered levels of H3K9ac following treatment. Importantly, we showed that dCas9-VP160 acts on patients´ cells in vitro, particularly the fibroblasts of patients with T1D.Gene Therapy advance online publication, 7 April 2016; doi:10.1038\/gt.2016.28.","palabras_clave":"EPIGENETICS|DIABETES|REPROGRAMMING|CRISPR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4293,"idioma_id":4,"titulo":"iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System","resumen":"When Dolly the sheep was born, the first probe into an adult mammalian genome traveling back in time and generating a whole new animal appeared. Ten years later, the reprogramming process became a defined method of producing induced pluripotent stem cells (iPSCs) through the overexpression of four transcription factors. iPSCs are capable of originating virtually all types of cells and tissues, including a whole new animal. The reprogramming strategies based on patient-derived cells should make the development of clinical applications of cell based therapy much more straightforward. Here, we analyze the current state, opportunities, and challenges of iPSCs from bench to bed, including organoids and the CRISPR system.","palabras_clave":"CRISPR|REPROGRAMMING|EPIGENETICS|IPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4294,"idioma_id":4,"titulo":"Effect of puerperal metritis on reproductive and productive performance in dairy cows in Argentina","resumen":"The objectives of this study were to evaluate the reproductive and productive performance of dairy cows with and without puerperal metritis and to evaluate the effectiveness of using a long-acting ceftiofur preparation. Dairy cows in one dairy farm, calving from July 2009 to January 2010 were examined between 3 and 14 days postpartum and classified based on vaginal discharge into three groups: cows with normal discharge (control; C); cows with a bloody mucus purulent or purulent non-fetid discharge (PnFD), and cows with bloody muco-purulent or purulent, fetid discharge (PFD). Cows in C and PnFD groups were not treated, whereas those in the PFD group were randomly allocated to receive 2.2 mg\/kg of ceftiofur subcutaneously behind the ear (PFD-T) or remain untreated (PFD-No T). From the 640 cows examined, 58.2% formed the C group, 13.4% formed the PnFD group, and 28.4% formed the PFD group. Survival curves differed between cows in the C group and PFD-No T group (P=0.0013) and between PFD-No T versus PFD-T group (P=0.0006). Survival curves of PnFD were intermediate and did not differ from those in the C group (P=0.2) and PFD-T group (P=0.1), but tended to be different from the PFD-No T group (P=0.056). The postpartum interval to achieve a 25% pregnancy rate was 72 days for cows in the C group, 73 days for the PFD-T group, 83 days for PnFD group, and 95 days for the PFD-No T group. The chance of pregnancy in a cow in the C group was 1.98 times higher (95% CI = 1.33, 3.08) and in cows in the PFD-T group was 2.16 times higher (95% CI = 1.37, 3.50) than those in the PFD-No T group. Finally, the chance of pregnancy in cows in the PnFD group tended to be higher (P=0.08) than those in the PFD-No T group, but did not differ from the other two groups. Cumulative 305-day milk production was higher (P <0.0001) in C group than those with vaginal discharge, regardless of fetidness and regardless of treatment. It is concluded that puerperal metritis affects the reproductive and productive performance of dairy cows and the treatment with ceftiofur was effective in reducing the adverse effects on reproductive performance, but not on milk production.","palabras_clave":"Metritis|Reproductive Performance|Dairy Cows|MIlk Production"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4295,"idioma_id":5,"titulo":"Contrastación de un programa de investigación científica progresivo en parasitología: los endoparásitos del zorro gris pampeano Lycalopex gymnocercus","resumen":"El objetivo de este artículo es contrastar y demostrar el poder explicativo y predictivo de un Programa de Investigación Científica en para-sitología tomando como evidencia empírica la parasitofauna del zorro gris pampeano (Lycalopex gymnocercus). El marco referencial epistemológico es la propuesta de Imre Lakatos que establece las características que un programa de investigación científica debe cumplimentar y se aplica a una disciplina biológica como es la parasitología, definiendo claramente sus componentes (núcleo tenaz, cinturón protector de hipótesis auxiliares y condiciones inicia-les), mostrando el valor heurístico para el trabajo presente y futuro en la disciplina. Las características biológicas de los taxones parasitarios hallados en el zorro gris pampeano permiten poner a prueba el programa de investigación científica en parasitología desarrollado. La propuesta basada en las peculiaridades tróficas de los hospedadores (intermediarios y definitivos) corrobora que el programa de investigación científica analizado es progresivo y aplicable a la teoría y la práctica parasitológica.","palabras_clave":"Educación|Filosofía|Parasitología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4296,"idioma_id":4,"titulo":"Seed dormancy QTL identification across a Sorghum bicolor segregating population","resumen":"Pre-harvest sprouting (PHS) in Sorghum bicolor is one of the main constrains for its production in the central region of Argentina, as grain maturation often coincides with rainy or high environmental humidity conditions. The obtention of more dormant genotypes with higher PHS resistance has always been a desirable trait for breeders but the typical quantitative nature of seed dormancy makes its manipulation difficult through classical breeding. Dissecting this quantitative variability into quantitative trait loci (QTL) is a main concern especially in cereal species. In this work, a sorghum segregating population including 190 families was genotyped with microsatellite markers and the SbABI5 candidate gene. A genetic map encompassing 96 markers and a total length of 1331 cM was built. Seed dormancy was phenotyped in F3 and F4 panicles in two contrasting Argentinean environments (Castelar and Manfredi). Six seed dormancy QTL for mature grains were identified (qGI-1, qGI-3, qGI-4, qGI-6, qGI-7 and qGI-9) with the aid of QTL Cartographer and QTLNetwork, three of them (qGI-3, qGI-7 and qGI-9) being co-localised by both approaches. No epistasis was detected for the identified QTL but QTL-by-environment interaction was significant for qGI-7 and qGI-9. Interestingly, seed dormancy candidate genes SbABI3\/VP1 and SbGA20ox3 were located within qGI-3, which makes them noteworthy candidate genes for this QTL.Keywords","palabras_clave":"SORGO|BROTADO PRECOSECHA|MAPA GENETICO|QTL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4298,"idioma_id":4,"titulo":"Alternative programs for synchronizing and re-synchronizing ovulation in beef cattle","resumen":"Fixed-time Artificial Insemination (FTAI) has been regarded as the most useful method to increase the number of cows inseminated in a given herd. The main treatments for FTAI in beef cattle are based on the use of progesterone-releasing devices and GnRH or estradiol to synchronize follicle wave emergence, with a mean pregnancy per AI (P\/AI) around 50%. However, more recent protocols based on GnRH (named 5-day Co-Synch) or estradiol (named J-Synch) that reduce the period of progesterone device insertion and extend the period from device removal to FTAI have been reported to improve P\/AI in beef cattle. Furthermore, treatments to re-synchronize ovulation for a second FTAI in non-pregnant cows have provided the opportunity to do sequential inseminations and achieve high P\/AI in a breeding season, reducing or even eliminating the need for clean-up bulls. In summary, FTAI protocols have facilitated the widespread application of AI in beef cattle, primarily by eliminating the necessity of estrus detection in beef herds.","palabras_clave":"Beef Cattle|Fixed Time Artifcial Insemination|Estradiol|GnRH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4299,"idioma_id":4,"titulo":"Facile preparation of silver-based nanocomposites via thiol-methacrylate 'click' photopolymerization","resumen":"Dispersions of silver nanoparticles (Ag NPs) in a tetrafunctional thiol were prepared byin situ reduction of silver nitrate with 2,6-di-tert-butyl-para-cresol. The obtained Ag NPs,were maintained in a stable colloidal state for more than eleven months at ambient temperature.The thiol monomer acts as both stabilizing agent and reactive solvent. Mixturesof dispersions of Ag NPs in thiol and a bifunctional methacrylate monomer were photoactivatedwith 2,2-Dimethoxy-2-phenylacetophenone (DMPA) or Camphorquinone (CQ) andthen photopolymerized by irradiation with UV or visible light respectively. Dispersionscontaining high amounts of Ag NPs were more efficiently photopolymerized with the pairCQ\/visible light. This is attributed to fact that the emission range of the UV LED(365 ± 5 nm) was overlapped with the surface plasmon resonance band of the Ag NPs(k &lt; 400 nm). Conversely, there was no overlap between the surface plasmon resonanceband of the Ag NPs and the emission range of the visible LED (peak at 470 nm).Consequently, most of the visible light was absorbed by CQ, while a fraction of the UV lightwas absorbed by the Ag NPs and was not available for the photoinitiation process.The thiol-methacrylate matrix of the nanocomposites was characterized by measuringthe glass transition temperature, the flexural modulus and the compressive strength. Thelow toxicity of the reactants used in the synthesis in combination with antimicrobialactivity of Ag NPs makes the nanocomposite materials synthesized in this study veryattractive for the preparation of biomaterials and coating with improved biocompatibility.","palabras_clave":"THIOL-ENE|PHOTOPOLIMERIZATION|SILVER NPS|NANOCOMPOSITES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4300,"idioma_id":4,"titulo":"Reduction of copper ions release by a novel ecofriendly electropolymerized nanolayer obtained from a natural compound (carvacrol).","resumen":"The release of copper ions by copper-containing devices, equipments and facilities represents a potential risk for biological systems. Different inhibitory treatments (CuIT) that use organic compounds have been proposed to reduce this environmental hazard but many of them are not in accordance with new regulations. The development of an ecofriendly CuIT based on the use of carvacrol, a natural phenolic compound present in essential oils, is reported here. The effects of carvacrol adsorption (adCarv) and its electropolymerization (polyCarv) were examined. Electropolymerization was attained after cycling the copper electrode in the 0.3?1.0 V potential range. Electrochemical techniques complemented by ATR-FTIR, XPS, SEM and AFM surface analyses were used to evaluate the composition and characteristics of the layers. Results demonstrated that adCarv includes cetonic structures while polyCarv additionally contains ether bonds. AFM and SEM observations showed the presence of round nanoglobules, larger for adCarv (close to 50 nm diameter). Cytotoxicity of adCarv and polyCarv layers on copper was also evaluated. The comparative analysis of both treatments revealed that polyCarv nanolayer is highly protective while the adCarv layer is weakly protective and reduction in cell viability was found. It was concluded that CuIT that leads to polyCarv nanolayer is very effective and ecofriendly.","palabras_clave":"Reduction copper ion release|Carvacrol|Electropolymerization|Ecofriendly"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4301,"idioma_id":4,"titulo":"Self-reinforced composites based on commercial PP woven fabrics and a random PP copolymer modified with quartz","resumen":"Self-reinforced composites based on commercial polypropylene (PP) woven fabrics and a random PP copolymermodified with quartz were obtained by film stacking. The effect of the incorporation of quartz on the materials fractureand failure behavior was studied through uniaxial tensile tests and quasi-static fracture experiments. Acousticemission analysis was also performed in situ in the tensile tests. A higher consolidation quality was obtained for thecomposites containing quartz. In the composite with random PP modified with 5 wt% quartz, the higher consolidationand the better dispersion of quartz particles positively impacted on the materials tensile and fracture behavior.From the results of acoustic emission analysis, fiber fracture appears as the dominant failure mechanism in theinvestigated composites.","palabras_clave":"POLYMER-MATRIX COMPOSITES|FRACTURE|MECHANICAL TESTING|ACOUSTIC EMISSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4302,"idioma_id":4,"titulo":"Aging in Water and in an Alkaline Medium of Unsaturated polyester and epoxy resins. Experimental study and modeling","resumen":"The change in stiffness with temperature in the presence of different media has been discussed for a long timebecause the ability to predict this behavior becomes fundamental to the design of new materials and their applications. That is why,in this work, the application of a mathematical model, which is able to predict the elastic properties of two polymers, is presented.The study takes into account the relationship between the viscoelastic and absorption properties of these materials in alkalinesolution (Ca (OH)2, pH 12.5) and in distilled water (H2O, pH 7) as the immersion media. Diffusion coefficient values were higherwhen the resins were immersed in water than in the alkaline solution. In addition, the effect of the alkaline medium was higher forthe unsaturated polyester resin (UPR). The highest decrease in modulus at the glassy state of the polymer network was observed forthe UPR immersed in the alkaline medium. The greatest reduction of the Tg value due to network plasticizing was found for theepoxy resin (ER) in the alkaline medium. Therefore, the ER exhibited a more stable behavior after aging at moderate temperaturethan the UPR.","palabras_clave":"composites|aging|fibres"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4303,"idioma_id":4,"titulo":"Normal state magnetotransport properties of beta-FeSe superconductors","resumen":"We present &#946;-FeSe magnetotransport data, and describe them theoretically. Usinga simplified microscopic model with two correlated effective orbitals, we determined the normal state electrical conductivity and Hall coefficient, using Kubo formalism. With model parameters relevant for Fe-chalcogenides, we describe the observed effect of the structural transition on the ab-plane electrical resistivity, as well as on the magnetoresistance. Temperature-dependent Hallcoefficient data were measured at 16 tesla, and their theoretical description improves upon inclusion of moderate electron correlations. We confirm the effect of the structural transition on the electronic structure, finding deformation-induced band splittings comparable to those reported in angle-resolved photoemission.","palabras_clave":"TRANSPORT PROPERTIES|PNICTIDES AND CHALCOGENIDES|THEORIES AND MODELS OF MANY ELECTRON SYSTEMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4304,"idioma_id":4,"titulo":"Synthesis of new double perovskites La1.98Mn1.11Mo0.89O5.93 and La1.92Mn1.29Mo0.71O5.84: Characterization of structural and magnetic properties","resumen":"The synthesis, structural and magnetic characterization of two new perovskites La1.98Mn1.11Mo0.89O5.93 and La1.92Mn1.29Mo0.71O5.84 are reported. Combined Rietveld refinements of X-ray and neutron powder diffraction data indicate that both samples are monoclinic perovskites with space group P21\/n. The distribution of the transition metals over the octahedral sites is highly ordered: Mn preferentially occupies 2c sites, while Mo mostly occupies 2d sites. Mn oxidation states were determined by means of high resolution Kb Emission Spectroscopy. The susceptibility as a function of temperature measurements and hysteresis loops show complex magnetic behaviour of both perovskites. The refined magnetic cells indicate that La1.98Mn1.11Mo0.89O5.93 is ferrimagnetic as a result of the antiferromagnetic coupling of two ferromagnetic sub-lattices associated to 2d and 2c sites, while La1.92Mn1.29Mo0.71O5.84 is ferromagnetic due to ferromagnetic alignment of the two sub-lattices. The magnetic overall behaviour of the latter perovskite is interpreted in terms of delocalization of magnetic moments arising from ferromagnetic double-exchange interactions between Mn2þ and Mn3þ.","palabras_clave":"MAGNETICALLY ORDERED MATERIALS|CRYSTAL STRUCTURE|VACANCY FORMATION|MAGNETIC MEASUREMENTS|NEUTRON DIFRACTION|ORDER DISORDER EFFECTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4305,"idioma_id":5,"titulo":"TRIPANOSOMIASIS AMERICANA EN UN CANINO DEL NORDESTE ARGENTINO. REPORTE DEL CASO CLINICO","resumen":"SE RELATA EL HALLAZGO DE UN PERRO DOMICILIARIO AFECTADO POR TRYPANOSOMA CRUZI EN CHACO (ARGENTINA) EN EL AÑO 2014, CORROBORANDO EL IMPORTANTE ROL DE LOS CANIDOS DOMESTICOS Y PERIDOMESTICOS EN LA TRANSMISION DEL MAL DE CHAGAS.","palabras_clave":"DOG,|DIAGNOSTIC CONFIRMATION| TRYPANOSOMA CRUZZI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4306,"idioma_id":4,"titulo":"The manus of Mylodon darwinii Owen (Tardigrada, Mylodontidae) and its phylogenetic implications","resumen":"The first nearly complete and articulated manus of the ground sloth Mylodon darwinii, found inUpper Pleistocene strata of Argentina, is described here. It shares similarities with Mylodonopsisibseni from Brazil, including a cuneiform with a distinct pisiform facet, an obliquely concaveulnar facet, and a prominent distolateral process, as well as a gracile metacarpal III. It shares aflattened pisiform with Glossotherium robustum. The trapezoid is unique in the obliquelyelongate proportions of its dorsal surface. Shapes of the articular facets indicate differentfunctions in digits II and III, with the former having a greater range of motion and the latter agreater stability at the joints. Clear arboreal or fossorial adaptations are absent. A phylogeneticanalysis recovered M. darwinii as closely related to M. ibseni and agrees with larger phylogeneticanalyses of sloths based on craniomandibular evidence. Our data support more than two events ofmylodontine dispersal to North America","palabras_clave":"Mylodon|hand|osteology|Pleistocene|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4308,"idioma_id":4,"titulo":"Effects on fibrinogen, fibrin, and blood coagulation of proteolytic extracts from fruits of Pseudananas macrodontes, Bromelia balansae, and B. hieronymi (Bromeliaceae) in comparison with bromelain","resumen":"Extracts rich in cysteine proteases obtained from fruits of Pseudananas macrodontes (Pm), Bromelia balansae (Bb), and B. hieronymi (Bh) have previously shown an anti-inflammatory effect on animal models. Given the close relationship between hemostasis and inflammation, it is attractive to investigate therapeutic agents capable of modulating both systems. The aim of this work was to study the effect of Pm, Bb, and Bh on fibrin(ogen) and blood coagulation compared with stem bromelain (Bro). Action on fibrinogen was electrophoretically and spectrophotometrically evaluated, fibrinolytic activity was measured both electrophoretically and by the fibrin plate assay, and the effect on blood coagulation was studied by conventional coagulation tests (PT and APPT). All extracts showed the same proteolytic preference for fibrinogen subunits, that is A&#945; > B&#946;, whereas &#947; was partially hydrolyzed by 100-fold concentration increase. Unlike Bro, cysteine proteases of Pm, Bb, and Bh increased absorbance at 540 nm of fibrinogen solution, suggesting thrombin-like activity, which was time-dependent and reached maximum values at lower concentration. All extracts showed the same proteolytic preference for fibrin subunits; however Pm, Bb, and Bh showed lower fibrinolytic activity than Bro at the assayed concentrations. Although Bb acted only as anticoagulant, Pm, Bh, and unexpectedly Bro showed dual action on blood coagulation: at low concentration showed procoagulant effect and at high concentration anticoagulant effect. Results reveal new plant species as potential sources of pharmacological agents for the treatment of a wide range of hemostatic disorders as well as to wound healing. © 2016 Wolters Kluwer Health, Inc. All rights reserved.","palabras_clave":"anticoagulant|bromelain|Bromeliaceae|cysteine protease|fibrinogenolytic|fibrinolytic|procoagulant"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4309,"idioma_id":4,"titulo":"Photophysical properties of \u0001\u0001Re(I)(CO)3(phen) pendants grafted to a poly-4-vinylpyridine backbone. A correlation between photophysical properties and morphological changes of the backbone","resumen":"Photochemical and photophysical properties of a polymer, Re-P4VP, consisting ofRe(I)(CO)3(phen)vpendants grafted to a poly-4-vinylpyridine backbone, P4VP, were interpreted on the bases ofvmorphological transformations. These transformations are responsible of a significant increase of the MLCTRe(I)!phen excited state luminescence lifetime when the polymer concentration is increased. Also a nearly 8-fold increase in the luminescence quantum yield resulted from the protonation of Re-P4VP with consequent changes of the excited state decay kinetics. Results of TEM and AFM morphological studies on P4VP and Re-P4VP in the presence of HClO4 acid, i.e., to form Re-P4VPHn n+, revealed that they haveconcentration dependent morphologies. From low to large concentrations of the Re-P4VP polymer, the morphology of Re-P4VP varies from a nonhomogeneous distribution of spherical nanoaggregates coexisting with micrometer size fibers to an homogeneous distribution of spherical nanoaggregates with diameters around 25 nm. The Re-P4VP morphology is also altered when the polymer pyridines are protonated. Protonation of diluted solutions of Re-P4VP polymers decrease the sizes of thenanoaggregates and small objects with diameters smaller than 10 nm appear","palabras_clave":"Photophysics|Poly(4-vinylpyridine) \u0001\u0001Re(CO)3(phen) pendants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4310,"idioma_id":4,"titulo":"Luminal Ca2+ dynamics during IP3R mediated signals","resumen":"The role of cytosolic Ca2+ on the kinetics of Inositol 1,4,5-triphosphatereceptors (IP3Rs) and on the dynamics of IP3R-mediated Ca2+ signals has been studiedat large both experimentally and by modeling. The role of luminal Ca2+ has not beeninvestigated with that much detail although it has been found that it is relevant forsignal termination in the case of Ca2+ release through ryanodine receptors. In thiswork we present the results of observing the dynamics of luminal and cytosolic Ca2+simultaneously in Xenopus laevis oocytes. Combining observations and modeling weconclude that there is a rapid mechanism that guarantees the availability of free Ca2+in the lumen even when a relatively large Ca2+ release is evoked. Comparing thedynamics of cytosolic and luminal Ca2+ during a release, we estimate that they areconsistent with a 80% of luminal Ca2+being buffered. The rapid availability of freeluminal Ca2+ correlates with the observation that the lumen occupies a considerablevolume in several regions across the images.","palabras_clave":"LUMINAL CALCIUM|IP3 RECEPTORS|CALCIUM SIGNALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4311,"idioma_id":4,"titulo":"P120-Catenin Regulates Early Trafficking Stages of the N-Cadherin Precursor Complex","resumen":"It is well established that binding of p120 catenin to the cytoplasmic domain of surface cadherinprevents cadherin endocytosis and degradation, contributing to cell-cell adhesion. Inthe present work we show that p120 catenin bound to the N-cadherin precursor, contributesto its anterograde movement from the endoplasmic reticulum (ER) to the Golgi complex. InHeLa cells, depletion of p120 expression, or blocking its binding to N-cadherin, increasedthe accumulation of the precursor in the ER, while it decreased the localization of mature Ncadherinat intercellular junctions. Reconstitution experiments in p120-deficient SW48 cellswith all three major isoforms of p120 (1, 3 and 4) had similar capacity to promote the processingof the N-cadherin precursor to the mature form, and its localization at cell-cell junctions.P120 catenin and protein tyrosine phosphatase PTP1B facilitated the recruitment ofthe N-ethylmaleimide sensitive factor (NSF), an ATPase involved in vesicular trafficking, tothe N-cadherin precursor complex. Dominant negative NSF E329Q impaired N-cadherintrafficking, maturation and localization at cell-cell junctions. Our results uncover a new rolefor p120 catenin bound to the N-cadherin precursor ensuring its trafficking through the biosyntheticpathway towards the cell surface.","palabras_clave":"P120-Catenin|N-Cadherin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4312,"idioma_id":5,"titulo":"Religión y niñez. Una revisión de las investigaciones en antropología","resumen":"Desde sus comienzos, la antropología haestado interesada, entre otros aspectos socioculturales, por el estudio de lareligión (Malinowski, 1948; Evans Pritchard, 1965) y éste continua siendo uncampo importante de indagación dentro de las ciencias sociales (Asad, 1993;Geertz, 1992; Bourdieu, 2006; Wright, 2008; Carozzi y Ceriani, 2007; Segato,2007). En el abordaje antropológico de la religión, no obstante, lasexperiencias de los\/as niños\/as han ocupado un lugar marginal. En este sentido,los\/as niños\/as no han sido considerados como 'informantes autorizados'(Gottlieb, 1998; García Palacios, 2006). Al mismo tiempo, existe una crecienteliteratura antropológica que analiza procesos que atraviesan los\/as niños\/as(Neufeld y Thisted, 1999; Cohn, 2005; Hecht, 2010). Sin embargo, la religiónpareciera ser una dimensión que sólo recién comienza a ser trabajada. Así, losestudios sobre religión y aquellos realizados acerca de la niñez y con niños\/asparecieran presentarse como dos campos escindidos (Gottlieb, 1998; GarcíaPalacios, 2012).A modo de estableceralgunas coordenadas para un diálogo posible entre estos campos, nos proponemosavanzar en la caracterización y análisis de ciertos aspectos relevantes delvínculo entre niños\/as y prácticas religiosas. Para ello, nos basaremos en larecopilación de bibliografía disponible de la antropología, a la vez querecuperaremos algunas producciones de disciplinas afines. El objetivo principalserá establecer algunos ejes analíticos que permitan acercarnos a un abordajecomparativo entre experiencias muy diversas, analizadas desde perspectivasteóricas heterogéneas. Pretendemos, pues, delimitar el campo de los debatesrelativos a las investigaciones centradas en la niñez y la religión, como unaforma de trazar un marco de análisis que sirva de referencia inicial encualquier trabajo preocupado por las experiencias formativas religiosas deniños y niñas. Cabe aclarar que lejos de querer elaborar una clasificación delas distintas investigaciones, nuestra intención es realizar una primerasistematización para reconstruir los modos en que son estudiadas lasvinculaciones entre la niñez y la religión, dando cuenta de los ejes que sonabordados en las investigaciones. En ese sentido, tal como veremos, los ejes deanálisis relevados no presentan un correlato excluyente en los diferentes estudiosaquí recopilados, así como tampoco deben considerarse en términosjerarquizados. En lo que sigue, proponemos focalizar el abordaje, por un lado,en aquellos estudios que relevan la participación de los\/as niños\/as enprácticas rituales y religiosas, y por el otro, en trabajos que reconstruyenlos puntos de vista de los\/as niños\/as sobre la religión","palabras_clave":"RELIGION|NIÑOS|PRACTICAS|ESTADO DEL ARTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4313,"idioma_id":4,"titulo":"Identification and characterization of maize salmon silks genes involved in insecticidal maysin biosynthesis","resumen":"The century-old Zea mays (maize) salmon silks mutation has been linked to theabsence of maysin. Maysin is a C-glycosyl flavone that, when present in silks, confersnatural resistance to the corn earworm (Helicoverpa zea), which is one of the mostdamaging pests of maize in America. Previous genetic analyses predicted PericarpColor1 (P1, R2R3-MYB transcription factor) to be epistatic to the sm mutation.Subsequent studies identified two loci as being capable of conferring salmon silksphenotypes, salmon silks 1 (sm1) and salmon silks 2 (sm2). Benefitting from availablesm1 and sm2 mapping information and from knowledge of the genes regulated by P1,we describe here the molecular identification of the Sm1 and Sm2 gene products. Sm2encodes a rhamnosyl transferase (UGT91L1) that uses isoorientin and UDP-rhamnoseas substrates, and converts them to rhamnosylisoorientin. Sm1 encodes a multidomainUDP-rhamnose synthase (RHS1) that converts UDP-glucose into UDP-L-rhamnose.Here, we demonstrate that RHS1 shows unexpected substrate plasticity in convertingthe glucose moiety in rhamnosylisoorientin to 4-keto-6-deoxy glucose, resulting inmaysin. Both Sm1 and Sm2 are direct targets of P1, as demonstrated by chromatinimmunoprecipitation (ChIP) experiments. The molecular characterization of Sm1 andSm2 described here completes the maysin biosynthetic pathway, providing powerfultools for engineering tolerance to corn earworm in maize and other plants.","palabras_clave":"flavonoide|maicina|maiz|insecticida"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4314,"idioma_id":4,"titulo":"Sex differences in the behavior of wild Alouatta caraya infants","resumen":"Several primates show sex-based differences in activity patterns and social interactions during infancy. These differences have been associated with adult social and reproductive functions of males and females and are related to male?male competition. Our goal was to describe behavioral patterns of wild Alouatta caraya male and female infants, a species with sexual dimorphism in body size and behavioral strategies during adulthood. We also examined the relationship between life history variables, infant sex and age, activity patterns, and social interactions in order to determine whether males and females follow different trajectories during early growth. Over a 27-month study, we observed 21 male infants and 14 female infants across two similar sites in northern Argentina. Data were analyzed using generalized linear mixed model (GLMM) tests. We found no differences in suckling time or weaning age between males and females (9.7 vs. 9.4 months), but male infants spent more time feeding on solid food and resting than female infants. Males also invested more time in contact with their mothers than did female infants, and mothers rejected and broke contact with males more frequently than with females. Other behavioral categories did not differ between the sexes. Our results suggest that higher nutritional demands of males compared with females may affect some behaviors. However, mothers of sons did not experience immediate trade-offs between current and future reproduction. Other behaviors, similarly expressed by the two sexes, suggest a similar developmental trajectory between male and female A. caraya infants, meaning that most differences emerge following the infant period.","palabras_clave":"ONTOGENY|BEHAVIORAL STRATEGIES|SOCIAL RELATIONSHIPS|SEX DIFERENCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4315,"idioma_id":4,"titulo":"Tuning the concentration of dye loaded polymer films for maximum photosensitization efficiency: phloxine B in poly(2-hydroxyethyl methacrylate)","resumen":"Fluorescence and singlet molecular oxygen (1O2) quantum yields for phloxine B loaded poly(2-hydroxyethyl methacrylate) thin films are determined at dye concentrations from 0.015 to 22 wt%. Fluorescence self-quenching and the fall off of the 1O2 quantum yield observed above 0.1 wt% are attributed to very weakly interacting close-lying dye molecules acting as energy traps arising from molecular confinement. The maximum singlet oxygen generation efficiency (quantum yield × absorption factor) lies at concentrations around 2 wt%, where fluorescence self quenching amounts to more than 80%. Data are fitted quantitatively by using a quenching radius model involving energy migration and trapping with rQ = 1.2 nm. The present results constitute a proof of concept for the rational design of heterogeneous photosensitizers in general and, particularly, for applications in which the antimicrobial activity of singlet oxygen is central.","palabras_clave":"POLYMER FILMS|DYES|SINGLET OXYGEN|EFFICIENCY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4316,"idioma_id":4,"titulo":"Translation and cross-cultural adaptation of a standardized international questionnaire on use of alternative and complementary medicine (I-CAM - Q) for Argentina.","resumen":"BACKGROUND:The widespread and growing use of alternative and complementary medicine (CAM) worldwide has been thoroughly described. In Argentina the limited information on the use of CAM has been reported between 40 and 55 %. However, the rate of use is extremely variable worldwide. For this purpose the international questionnaire on the use of complementary and alternative medicines (I-CAM - Q), was developed. The implementation of a translated and cross-culturally adapted version of the questionnaire would allow for a reliable and standardized evaluation of the rate of use of CAM in Argentina. It would be a great step towards improving what we know about the healing habits of our population.METHODS:The forward and back-translation method was used. Four translators were involved. A committee was commissioned to reconcile the different versions. The process of cross-cultural adaptation was made by consulting 17 alternative and complementary medicine experts using the DELPHI method. The retrieved questionnaire was evaluated in 18 patients sampled by convenience (9 men, different educational and self-reported health levels). The interviews consisted of three parts: an initial demographics questionnaire; the administration of the I-CAM-Q and finally the cognitive interview, which included reviewing the questionnaire and reexamining questions that generated doubts during the interview. The comprehension of the questions was also evaluated. As a last step, using the information obtained from the interviews, the final version of the questionnaire was drafted.RESULTS AND CONCLUSION:The questionnaire seems to have been accepted by most patients during the interviews. Conflictive elements that emerged did not seem to have an impact on its administration. The flexibility of the questionnaire allowed to add professionals and practices which contributed to a more accurate local adaptation. Further research should focus on assessing the questionnaire's psychometric performance and validity, which so far has not been done.","palabras_clave":"Alternative medicine|Complementary medicine|Questionnaire"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4317,"idioma_id":4,"titulo":"Modeling magnetization curves in magnetic thin films with striped patterns","resumen":"In this work, we study magnetic thin films presenting magnetic stripe patterns. A fingerprint of such domains is a linear behavior of the in-plane magnetization curves below a given saturation field. We present free energy models for the in-plane magnetization curves which permit us to extract key geometrical information about the stripe patterns, such as the maximum canted angle of the magnetization and the domain wall width. As an example, we discuss in this work magnetization curves for Fe1&#8722;x Ga x magnetic films which present a stripe pattern with a period of 160&#8201;nm and we found a typical maximum canted angle of ${{85}^{{}^circ}}$  and a domain wall width around 30&#8201;nm.","palabras_clave":"magnetic thin films|magnetic patterns|magnetostrictive materials|magnetic free energy models"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4318,"idioma_id":4,"titulo":"Major cereal crops benefit from biological nitrogen fixation when inoculated with the nitrogen-fixing bacterium Pseudomonas protegens Pf-5 X940","resumen":"Abstract A main goal of biological nitrogen fixation (BNF)research has been to expand the nitrogen fixing ability to major cereal crops.In this work, we demonstrate the use of the efficient nitrogen-fixing rhizobacterium Pseudomonas protegens Pf-5 X940 as a chassis to engineer the transfer of nitrogen fixed by BNFto maize and wheat under non-gnotobiotic conditions. Inoculation of maize and wheat withPf-5 X940 largely improved nitrogen content and biomass accumulation in bothvegetative and reproductive tissues, and this beneficial effect waspositively associated with high nitrogen fixation rates in roots. 15N isotopedilution analysis showed that maize and wheat plants obtained substantial amountsof fixed nitrogen from the atmosphere. Pf-5 X940-GFP-tagged cells were always reisolatedfrom the maize and wheat root surface but never from the inner root tissues. Confocal laserscanning microscopy confirmed root surface colonization of Pf-5 X940-GFP in wheat plants,and microcolonies were mostly visualized at the junctions between epidermal root cells.Genetic analysis usingbiofilm formation-related Pseudomonas mutants confirmed the relevance of bacterial root adhesionin the increase in nitrogen content, biomass accumulation and nitrogen fixationrates in wheat roots. To our knowledge, this is the first report of robust BNFin major cereal crops.","palabras_clave":"Pseudomonas|Nitrogen fixation|Recombinant|Crops"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4319,"idioma_id":4,"titulo":"Network dynamics: quantitative analysis of complex behavior in metabolism, organelles and cells, from experiments to models and back","resumen":"Advancing from two core traits of biological systems: multilevel network organization and nonlinearity, we review a host of novel and readily available techniques to explore and analyze their complex dynamic behavior within the framework of experimental-computational synergy. In the context of concrete biological examples, analytical methods such as wavelet, power spectra, and metabolomics-fluxomics analyses, are presented, discussed, and theirstrengths and limitations highlighted. Further shown is how time series from stationary and non-stationary biological variables and signals, such as membrane potential, high throughput metabolomics, O2 and CO2 levels, bird locomotion, at the molecular, (sub)cellular, tissue, and whole organ and animal levels, can reveal important information on the properties of the underlying biological networks. Systems biology-inspired computational methods start to pave the way for addressing the integrated functional dynamics of metabolic, organelle and organ networks. As our capacity to unravel the control and regulatory properties of these networks and their dynamics under normal or pathological conditions broadens, so is our ability to address endogenous rhythms and clocks to improve health-span in human aging, and to manage complex metabolic disorders, neurodegeneration, and cancer.","palabras_clave":"NETWORKS|CHAOS|NONLINEAR DYNAMICS|FRACTALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4320,"idioma_id":4,"titulo":"Presence of New Delhi metallo-&#946;-lactamase gene (NDM-1) in a clinical isolate of Acinetobacter junii in Argentina","resumen":"In the last few years, Acinetobacter infections caused by membersof this genus other than baumannii have been recognizedas a result of the implementation of new technologies in diagnosticlaboratories. Acinetobacter junii is an atypical human pathogenthat has been mainly associated with bacteraemia inneonates and paediatric oncology patients. Some cases of meningitis,peritonitis, ocular infection and septicaemia caused byA. junii have been reported [1]. Moreover, many recently publishedreports of Acinetobacter spp. harbouring blaNDM suggestedAcinetobacter as the source and cause of spread for this threateningcarbapenemase [2?6]. The identification of blaNDM-1 wasrecently described in A. junii clinical isolates from China","palabras_clave":"new delhi|horizontal gene transfer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4321,"idioma_id":5,"titulo":"Efecto de iones metálicos y compuestos aromáticos sobre la actividad lacasa en Trametes sp. nativo de Misiones","resumen":"Los hongos de pudrición blanca secretan enzimas oxidativas, como la lacasa, con capacidad de intervenir en procesos amigables con el medioambiente, tales como la biorremediación. El objetivo del presente trabajo fue formular un medio de cultivo suplementado con compuestos aromáticos e iones metálicos con el fin de aumentar los valores de actividad lacasa expresados por Trametes sp. cepa A. Utilizando un diseño de Plackett-Burman se logró establecer que el Mn2+ fue la única variable que tuvo un efecto significativo positivo sobre la actividad. La mayor actividad lacasa (272,46 U\/L) se obtuvo a una concentración de 1,5 mM de Mn2+ y la máxima biomasa (0,1272 g) a una concentración de 4 mM de Mn2+. Asimismo fue posible establecer una correlación negativa entre la actividad enzimática y el crecimiento del hongo. Se encontró una isoenzima de 60 kDa de expresión constitutiva y otra de 100 kDa inducible en presencia de 2; 2,5; 3; 3,5 y 4 mM de Mn2+.","palabras_clave":"ACTIVIDAD LACASA|HONGOS DE PUDRICION BLANCA|TRAMETES|ISOENZIMAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4322,"idioma_id":5,"titulo":"?\tInvestigación sobre procesos de validación en entornos de geometría dinámica","resumen":"El interés por capacitar tecnológicamente a los futuros ingenieros es una tarea a cumplir desde los primeros años de las carreras.Actualmente se realizan en el mundo investigaciones que persiguen distintas finalidades referidas a la incorporación de software enmaterias de ciencias básicas.En la Universidad Tecnológica Nacional (UTN) Regional Pacheco, en las carreras de Ingeniería, hoy en día se pretende incluir en laenseñanza de la Matemática de 1º año el uso de un software de Geometría Dinámica (SGD): el Cabri.La investigación que hemos concluido a fines del año 2012 tuvo, entre otros objetivos, los de estudiar los procesos de validación que ponen en juego los estudiantes en un contexto mediado por SGD, y explorar los vínculos que ellos establecen entre su uso y los recursos y acciones que pueden desplegar en situaciones de validación. Es nuestra intención compartir los aspectos más relevantes de la investigación desarrollada, con el fin de clarificar los vínculos entre el uso de SGD y el desarrollo de herramientas conceptuales básicas, como lo son las relativas a la validación matemática. Este aspecto es constitutivo de la Matemática y, además, su aprendizaje permite que gradualmente los estudiantes logren autonomía y confianza en lo que concierne a la fundamentación de sus estrategias. El trabajo se llevó a cabo con estudiantes de la asignatura Álgebra y Geometría Analítica, y el contenido involucrado fuetransformaciones lineales.","palabras_clave":"TRANSFORMACIONES LINEALES|VALIDACION MATEMÁTICA|GEOMETRÍA DINÁMICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4323,"idioma_id":4,"titulo":"Metabolic profile of Mycobacterium smegmatis reveals Mce4 proteins are relevant for cell wall lipid homeostasis","resumen":"IntroductionThe Mce proteins are encoded in a variable number of operons (from one to eight) in all Mycobacterium species. A role in the transport of host and mycobacterial lipids has been demonstrated for some Mce proteins in the pathogen Mycobacterium tuberculosis but little is known about these proteins in Mycobacterium smegmatis, a soil dweller species.ObjectiveTo investigate the role of Mce proteins in M. smegmatis.MethodWe used a non-targeted GC?MS approach to define the metabolic profiles of knockout mutants in mce operons of M. smegmatis. Metabolomic analysis was complemented with thin layer chromatography and transcriptional studies.ResultWe demonstrated that the lack of Mce4 proteins alters the primary carbon metabolism of M. smegmatis by reducing the content of fatty acids and increasing the accumulation of two stress-related compounds, glutamate\/glutamine and triacyl glycerol (TAG). We also provide evidence supporting that the accumulation of TAG in a &#916;mce4 mutant depends on the bacterial redox state.ConclusionThese results, together with the finding that the cell surface of the &#916;mce4 mutant is significantly altered, support a role for Mce4 in maintaining the cell wall lipids architecture of M. smegmatis, which, when altered, induces a redox imbalance that results in the accumulation of the stress-related compounds.","palabras_clave":"Mycobacterium smegmatis|mce4 operon|Triacyl glycerol |Glutamate"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4324,"idioma_id":4,"titulo":"The genomics of mycobacteria","resumen":"The species Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis are the causal agents, respectively, of tuberculosis and paratuberculosis in animals. Both mycobacteria, especially M. bovis, are also important to public health because they can infect humans. In recent years, this and the impact of tuberculosis and paratuberculosis on animal production have led to significant advances in knowledge about both pathogens and their host interactions. This article describes the contribution of genomics and functional genomics to studies of the evolution, virulence, epidemiology and diagnosis of both these pathogenic mycobacteria. ","palabras_clave":"Diagnostic|Epidemiology |Evolution |Genomics |Mycobacteria |Paratuberculosis |Tuberculosis |Virulence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4325,"idioma_id":4,"titulo":"Changes in the redox status of the brain in an experimental glaucoma model","resumen":"The objective was to measure changes in the redox status with the means of evaluating the antioxidative state of the primary visual structures on the brain of a rat glaucoma model. The animal model consisted of inducing ocular hypertension by cauterizing two episcleral veins on the left eye. The markers of oxidative damage and the oxidative balance evaluated in the brain seven days postoperative were: nitrites concentration, levels of non-enzymatic antioxidants and antioxidant enzymes activity.The active species of both oxygen and nitrogen increased in glaucoma group and they could bring, as a result of their actions, an increase on the damage to lipids and proteins. The decrease on the non-enzymatic antioxidants and the compensatory increase of the superoxide dismutase and glutathione peroxidase activities could be a consequence of the increase of oxidative processes. The decrease in the activity of glutathione reductase leads to a decrease in the recycling of thiol groups.We suggest that oxidative stress can possibly acts as a risk factor for neurodegeneration in the brain. Therapeutic strategies to stop the progression of the disease in glaucoma should also consider the central neuronal degeneration beyond the retina and the optic nerve.","palabras_clave":"GLAUCOMA|OXIDATIVE STRESS|BRAIN|REDOX STATUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4326,"idioma_id":4,"titulo":"Dynamical Casimir effect in superconducting circuits: a numerical approach","resumen":"We present a numerical analysis of the particle creation for a quantum field in the presence of time-dependent boundary conditions. Having in mind recent experiments involving superconducting circuits, we consider their description in terms of a scalar field in a one-dimensional cavity satisfying generalized boundary conditions that involve a time-dependent linear combination of the field and its spatial and time derivatives. We evaluate numerically the Bogoliubov transformation between in- and out-states and find that the rate of particle production strongly depends on whether the spectrum of the unperturbed cavity is equidistant or not, and also on the amplitude of the temporal oscillations of the boundary conditions. We provide analytic justifications for the different regimes found numerically.","palabras_clave":"Efecto Casimir|Generación de fotones|Circuitos superconductores"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4327,"idioma_id":4,"titulo":"Construction and in vitro testing of a cellulose dura mater graft","resumen":"Introduction: Cerebrospinal fluid (CSF) leaks are a common complication after cranial and spinal surgery and are associated with increased morbidity. Despite continuous research in this field, this problem is far from solved. In this paper, we describe the construction and testing of a bacterial cellulose (BC) membrane as a new dural patch.Materials and Methods: The synthesis of BC was performed using Gluconacetobacter hansenii (ATCC 23769) and films were sterilized by autoclaving. The membranes were seeded with human dural fibroblasts. Growth, shape, and cell viability were assessed after 4 weeks.Results: Normally shaped fibroblasts were seen on the BC grafts; confocal microscopy showed cells inside the structure of the mesh. Both viable and nonviable cells were present. Cellular attachment and viability were confirmed by replating of the membranes.Discussion: BC membranes are used in clinical practice to improve skin healing. In the presence of water, they form an elastic, nontoxic, and resistant biogel that can accommodate collagen and growth factors within their structure, thus BC is a good candidate for dural graft construction.","palabras_clave":"cerebrospinal fluid leak|dura mater patch|bacterial cellulose membrane"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4328,"idioma_id":4,"titulo":"Triton Hodge Test: Improved Protocol for Modified Hodge Test for Enhanced Detection of NDM and Other Carbapenemase Producers","resumen":"Accurate detection of carbapenemase-producing Gram-negative bacilli is of utmost importance for the control of nosocomialspread and the initiation of appropriate antimicrobial therapy. The modified Hodge test (MHT), a carbapenem inactivation assay,has shown poor sensitivity in detecting the worldwide spread of New Delhi metallo--lactamase (NDM). Recent studiesdemonstrated that NDM is a lipoprotein anchored to the outer membrane in Gram-negative bacteria, unlike all other knowncarbapenemases. Here we report that membrane anchoring of -lactamases precludes detection of carbapenemase activity bythe MHT. We also show that this limitation can be overcome by the addition of Triton X-100 during the test, which allows detectionof NDM. We propose an improved version of the assay, called the Triton Hodge test (THT), which allows detection of membrane-bound carbapenemases with the addition of this nonionic surfactant. This test was challenged with a panel of 185 clinicalisolates (145 carrying known carbapenemase-encoding genes and 40 carbapenemase nonproducers). The THT displayed testsensitivity of&gt;90% against NDM-producing clinical isolates, while improving performance against other carbapenemases. Ertapenemprovided the highest sensitivity (97 to 100%, depending on the type of carbapenemase), followed by meropenem (92.5to 100%). Test specificity was not affected by the addition of Triton (87.5% and 92.5% with ertapenem and meropenem, respectively).This simple inexpensive test confers a large improvement to the sensitivity of the MHT for the detection of NDM andother carbapenemases.","palabras_clave":"Triton Hodge Test|Protocol|NDM|Carbapenemase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4329,"idioma_id":4,"titulo":"Ecophysiology of environmental Aspergillus fumigatus strains at different aW and pH conditions. Comparison of gliotoxin production and elastase activity among environmental and clinical strains","resumen":"AbstractThe aim of this manuscript was to study the influence of water activity (aW ) and pH in the ecophysiological behaviour of Aspergillus fumigatus strains at human body temperature. In addition, gliotoxin production and enzymatic ability among environmental (n = 2) and clinical (n = 5) strains were compared. Ecophysiological study of environmental strains was performed on agar silage incubated at 37°C, studying the interaction at eight aW levels (0·8, 0·85, 0·9, 0·92, 0·94, 0·96, 0·98 and 0·99) and eight pH levels (3·5, 4, 4·5, 5, 6, 7, 7·5 and 8). Considering the influence of the assumed lung conditions on growth of A. fumigatus (aW 0·98\/0·99 and pH of 7\/7·5), the optimal condition for the development of A. fumigatus RC031 was at aW 0·99 at pH 7. At aW 0·98\/0·99 and pH of 7\/7·5, the highest growth rate and the lowest lag phase was reported, whereas there were no significant differences at aW 0·98\/0·99 and pH 7\/7·5 interactions on growth of A. fumigatus RC032. Gliotoxin production of A. fumigatus strains was evaluated. The gliotoxin production was similar in clinical and environmental strains. Elastin activity was studied in solid medium, highest elastase activity index was found for clinical strain A. fumigatus RC0676, followed by the environmental strain A. fumigatus RC031. Opportunistic environmental strains can be considered as pathogenic in some cases when rural workers are exposed constantly to handling silage.SIGNIFICANCE AND IMPACT OF THE STUDY:Aspergillus fumigatus is one of the main opportunist pathogen agents causing invasive aspergillosis. Rural workers present a constant exposition to A. fumigatus spores caused by feed-borne manipulation. In this study, environmental A. fumigatus strains were able to grow and produce gliotoxin onto the studied conditions including the lung ones. Environmental and clinical strains were physiologically similar and could be an important putative infection source in rural workers.","palabras_clave":"Aspergillus fumigatus|aW|elastase activity|environment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4330,"idioma_id":5,"titulo":"Una nueva psicología del razonamiento : fundamentos y aplicaciones","resumen":"Se ha anunciado recientemente la creación de un Nuevo Paradigma en psicología del razonamiento.El propósito del presente artículo es presentar sus fundamentos y aplicaciones. Se proponen reflexiones críticas sobre las innovaciones respecto del paradigma previo. Se consideran tres fundamentos: 1) el empleo de una nueva lógica normativa que contempla la verdad, la falsedad y otras opciones, 2) el empleo de nuevos recursos formales como la probabilidad subjetiva para modelar fenómenos psicológicos y, 3) el recurso al marco teórico general de la mente dual. Se analizan también las potenciales aplicaciones del Nuevo Paradigma. Por último, se mencionan algunos avances. Se concluye que el Nuevo Paradigma es un modelo original de prácticas para la investigación sobre razonamiento deductivo que se encuentra en elaboración.fundamentos, aplicacionesAbstractt","palabras_clave":"RAZONAMIENTO|PARADIGMA|FUNDAMENTOS|APLICACIONES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4331,"idioma_id":4,"titulo":"First report of predation by a small shark on the invasive rapa whelk Rapana venosa (Valenciennes, 1846) in Argentinean waters","resumen":"The rapa whelk Rapana venosa (Valenciennes, 1846) has few predators in areas outside of its native range. Here we report the first incidenceof predation by a small shark Mustelus schmitti Springer, 1939 on the Rapa whelk in coastal waters of Argentina. This finding could giveinformation about possible trophic control over R. venosa as it potentially disperses into coastal waters of Atlantic South America.","palabras_clave":"ARGENTINA|SHARKS|DIET|GASTROPODS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4332,"idioma_id":4,"titulo":"Young's (in)equality for compact operators","resumen":"If $a,b$ are $n\\times n$ matrices, Ando proved that Young's inequality is valid for their singular values: if $p&gt;1$ and $1\/p+1\/q=1$, then$$\\lambda_k(|ab^*|)\\le \\lambda_k\\left( \\frac1p |a|^p+\\frac 1q |b|^q \\right) \\, \\textit{ for all }k.$$Later, this result was extended for the singular values of a pair of compact operators acting on a Hilbert space by Erlijman, Farenick and Zeng. In this paper we prove that if $a,b$ are compact operators, then equality holds in Young's inequality if and only if $|a|^p=|b|^q$. Therefore we&nbsp; obtain a complete characterization of such $a,b$ in relation to other (operator norm) Young inequalities, related to operator ideals.","palabras_clave":"YOUNG INEQUALITY|COMPACT OPERATOR|SINGULAR VALUE|SPECTRUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4333,"idioma_id":5,"titulo":"CARACTERIZACIÓN HIGIÉNICO, SANITARIA Y COMPOSICIONAL DE  LA LECHE PRODUCIDA POR ESTABLECIMIENTOS LECHEROS DE LA PROVINCIA DE LA PAMPA.","resumen":"Mediante el  análisis de leche cruda de tanque, se determinó la calidad higiénico, sanitaria y composicional de la leche producida en los tambos de la Cuenca Lechera de La Pampa, para compararla con los valores medios obtenidos con los óptimos acordados entre la producción primaria y la industria en el ?Sistema de Pago de la Leche Cruda sobre la base de atributos de Calidad Composicional e Higiénico-Sanitarios en el Sistema de Liquidación Única, Mensual, Obligatoria y Universal? del Ministerio de Agricultura, Ganadería y Pesca y Economía de la Nación (Magyp). Los valores óptimos de calidad  acordados en el Magyp son: &#8805; 3,45 % de Grasa Butirosa, &#8805; 3,15 % de Proteína, Crioscopía -0,512, no mayor a 400.000 CCS\/ml y 100.000 UFC\/ml. Temperatura 4°C, sin presencia de Inhibidores, y libre de Brucelosis y Tuberculosis. Se seleccionaron 52 tambos distribuidos en las tres sub-cuencas pampeanas (norte, centro y sur). Durante 2 años (2013 - 2014). Los resultados promedios de  composición de leche fueron de 3,72% de grasa butirosa (GB) y 3,43 de proteína (PR). Estos valores obtenidos fueron un 9 % más que los valores de referencia. En relación a la calidad higiénico-sanitaria los datos obtenidos fueron  383.000 CCS\/ml y 54.000 UFC\/ml, ambos acordes a los estándares establecidos. En ninguno de los tambos estudiados se detectaron valores más elevados a la temperatura de entrega, sin aguado de la leche y ausencia de inhibidores. Sin embargo el 31% de los establecimientos evaluados mostró valores superiores a las 400.000 CCS\/ml, indicativo de problemas sanitarios de ubre (mastitis), y el 10% de los tambos presentó problemas de higiene con valores promedios superiores a las 100.000 UFC\/ml. Podemos concluir que la mayoría de los establecimientos pampeanos produce leche de buena calidad composicional e higiénico ? sanitaria, y un sector menor requiere medidas correctivas para mejorar dichos parámetros. La información obtenida será de utilidad para implementar políticas de mejoramiento de calidad de leche en la provincia, tanto en la producción primaria como en la industria.","palabras_clave":"Caracterización|Calidad de leche|Tambos|La Pampa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4334,"idioma_id":4,"titulo":"Soil erodibility and quality of volcanic soils as affected by pine plantations in degraded rangelands of NW Patagonia","resumen":"Abstract NW Patagonia in Argentina has high potentialfor planting fast-growing exotic conifers, supported by itsvolcanic soils. Nonetheless, many aspects related to theeffects of pine plantations on soil are still unknown. Weaimed to evaluate the quality and erodibility of volcanicsoils under the hypothesis that Pinus ponderosa plantationsincrease the quality and decrease the erosion rate of soilscompared to degraded rangelands. Rainfall simulationexperiments were performed in degraded rangeland soilsand in pine plantations with none, partial and completeremoval of fresh litter and duff layers. Results showed thatrangeland soils were highly susceptible to water erosion.Sediment production in the rangeland varied between 144and 750 g m-2. Loamy sand soils, poor in organic matter(OM) and without non-crystalline aluminosilicates, werethe most erodible soils. The plantations improved soilquality, with positive changes in OM content and total andeffective porosity, mainly in soils without non-crystallinematerials. Soil erosion in pine plantations was negligiblewhen fresh litter was either conserved or removed, witherosion rates as low as 6.2 ± 1.5 and 23.7 ± 7.9 g m-2,respectively. Even when fresh litter and duff layers weretotally removed, soil erosion rates in the pine plantations(129.1 ± 23.2 g m-2) were lower than in the rangelandsites; however, this reduction was significant only for themost erodible soils. The high erodibility of volcanic soilsand the low soil cover in overgrazed rangelands revealedthe fragility of the soils in the study area. We show thatpine plantations, an alternative land use of rangelands,improve some aspects of soil quality, provide a mulchingeffect through the litter layer and became a mean forcontrolling soil erosion.","palabras_clave":"Soil erosion|Runoff|Simulated rainfall|Pinus ponderosa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4335,"idioma_id":4,"titulo":"Functionalized polyanilines made by nucleophilic addition reaction, applied in gas sensors field","resumen":"Resistive sensors for organic volatile compounds are built using functionalized polyanilines. The functionalization of polyaniline (PANI) is achieved by nucleophilic addition with thiols and is confirmed by X-ray Photoelectron Spectroscopy. The modified polymers tested as resistive sensors change the polymer sensitivity to volatile compounds. PANI shows a signal that increases when the chain length increases, however, functionalized polyanilines show a completely different behaviour. The sensitivity to long chain alcohols increases when PANI is modified with long alkyl chains, the opposite behaviour occurs when more hydrophilic groups are bonding to the polymer. We conclude that the different sensitivity of the functionalized polyanilines to the same volatile molecule allows identifying the analyte. In that way, multisensor arrays based on these materials could be used as an electronic nose. To explain this behaviour measurements of contact angle were performed. The data reveal that all modified polyanilines are more hydrophobic than PANI, this is likely to be due to the presence of the sulphur atoms. Therefore, we conclude that the hydrophobic\/hydrophilic nature of the surface plays an important role but is not decisive in the sensor response. Also, Van der Waals interactions between the alkyl chains bound to de PANI and present in the sensed alcohols play an important role. Accordingly, the sensitivity for a plain alkane (heptane) increases strongly when PANI is modified with long alkyl chains.","palabras_clave":"Modified polymers|Polyaniline|Organic volatile compounds|Resistive sensors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4336,"idioma_id":4,"titulo":"Hybrid Biomaterials Based on Calcium Carbonate and Polyaniline Nanoparticles for Application in Photothermal Therapy","resumen":"Inorganic materials contain remarkable properties for drug delivery, such as a large surfacearea and nanoporous structure. Among these materials, CaCO3 microparticles (CMPs) exhibita high encapsulation efficiency and solubility in acidic media. The extracellular pH of tumorneoplastic tissue is significantly lower than the extracellular pH of normal tissue facilitatingthe release of drug-encapsulating CMPs in this area. Conducting polyaniline (PANI) absorbslight energy and transforms it into localized heat to produce cell death. This work aimed togenerate hybrid CMPs loaded with PANI for photothermal therapy (PTT). The hybridnanomaterial was synthesized with CaCO3 and carboxymethyl cellulose in a simple,reproducible manner. The CMP-PANI-Cys particles were developed for the first time andrepresent a novel type of hybrid biomaterial. Resultant nanoparticles were characterizedutilizing scanning electron microscopy, dynamic light scattering, zeta potential, UV-Vis,FTIR and Raman spectroscopy. In vitro HeLa cells in dark and irradiated conditions showedthat CMP-PANI-Cys and PANI-Cys are nontoxic at the assayed concentrations. Hybridbiomaterials displayed high efficiency for potential PTT compared with PANI-Cys. Insummary, hierarchical hybrid biomaterials composed of CMPs and PANI-Cys combined withnear infrared irradiation represents a useful alternative in PTT","palabras_clave":"Biological applications of polymers|Biomineralization|Drug delivery systems| Nanocomposites|Nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4337,"idioma_id":4,"titulo":"Sulfonated porous carbon catalysts for biodiesel production: Clear effect of the carbon particle size on the catalyst synthesis and properties","resumen":"Sulfonated porous carbons (PCs-SO3H) are prepared by the sol-gel reaction of resorcinol and formaldehyde. The porosity is maintained during drying using a cationic polyelectrolyte as pore stabilizer. It was found that varying the Resorcinol\/Na2CO3 molar rate, different resins are produced which, after pyrolysis, give carbonaceous materials with different textural properties. It seems that a Resorcinol\/Na2CO3 molar ratio of 200:1 was the optimal condition to produce a well-developed porous structure. Both resins and carbon materials are sulfonated by treatment with sulphuric acid. The relation between the carbon particles size, the sulfonation efficiency and its performance as a catalyst is studied. The higher amount of sulfonic groups, and thermal stability, as well as better catalytic performance, was obtained when smaller porous carbon particles were chosen by sieving. PCs-SO3H exhibited high efficiency for the esterification reaction and high performance for biodiesel production. The catalysts can be recycled several times with a minimal loss of activity. Thermal analysis evidenced stability up to ca. 200 &deg;C, allowing the use of this catalyst at high temperature. The simple synthesis and low cost of the PCs-SO3Hs make them promising catalysts for the synthesis of biodiesel.","palabras_clave":"BIODIESEL|CATALIZADORES HETEROGENEOS|CARBON POROSO|CATALISIS ACIDA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4338,"idioma_id":4,"titulo":"IgY against enterotoxigenic Escherichia coli administered by hydrogel-carbon nanotubes composites to prevent neonatal diarrhoea in experimentally challenged piglets","resumen":"In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin(IgY) against simulated gastric conditions was established. Thereafter, the performance of thehydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicityfor porcine intestinal cells of these nanocomposites was assessed. The objective of the present study wasto evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulinis used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets. Groups of piglets orallychallenged with 1011 CFU\/mL of ETEC were treated with non-protected and protected IgY. The clinicalresponse of each group was monitored and evaluated in terms of dehydration, rectal temperature, faecalconsistency score and body weight gain. Blood parameters and histological aspects were also studied. Theresults showed that treatment of infected piglets with protected IgY reduced significantly the severity ofdiarrhea. Non-protected IgY group show a lower recovery rate. Blood parameters and histological aspectswere normal in both groups. Collectively, these results support previous in vitro studies showing thatthe nanocomposites can be an effective method of IgY protection against gastric inactivation.","palabras_clave":"EGG YOLK IMMUNOGLOBULIN|ENTEROTOXIGENIC ESCHERICHIA COLI|HYDROGEL-CARBON NANOTUBES COMPOSITES|NEONATAL PIGS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4339,"idioma_id":4,"titulo":"Differential Left Hippocampal Activation during Retrieval with Different Types of Reminders: An fMRI Study of the Reconsolidation Process","resumen":"Consolidated memories return to a labile state after the presentation of cues (reminders)associated with acquisition, followed by a period of stabilization (reconsolidation). However not all cues are equally effective in initiating the process, unpredictable cues triggered it,predictable cues do not. We hypothesize that the different effects observed by the different reminder types on memory labilization-reconsolidation depend on a differential neural involvement during reminder presentation. To test it, we developed a declarative task andcompared the efficacy of three reminder types in triggering the process in humans (Experiment 1). Finally, we compared the brain activation patterns between the different conditions using functional magnetic resonance imaging (fMRI) (Experiment 2). We confirmed that the unpredictable reminder is the most effective in initiating the labilization-reconsolidation process.Furthermore, only under this condition there was differential left hippocampal activationduring its presentation.We suggest that the left hippocampus is detecting theincongruence between actual and past events and allows the memory to be updated.","palabras_clave":"memory reconsolidation|declarative memory|fMRI|left hippocampus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4340,"idioma_id":4,"titulo":"The dynamic nature of the reconsolidation process and its boundary conditions: Evidence based on human tests","resumen":"The reconsolidation process is the mechanism by which the strength and\/or content of consolidated memories are updated. This process is triggered by the presentation of a reminder (training cues). It is not always possible to trigger the reconsolidation process. For example, memory age and strength are boundary conditions for the reconsolidation process. Here, we investigated the dynamic changes in these conditions. We propose that the boundary conditions of the reconsolidation process are not fixed and vary as a consequence of the interaction between memory features and reminder characteristics. To modify memory properties, participants received a threatening social protocol that improves memory acquisition or a control condition (fake, without social interaction) prior to learning pairs of meaningless syllables. To determine whether a strong young or old declarative memory undergoes the reconsolidationprocess, we used an interference task (a second list of pairs of meaningless syllables) to disrupt memory re-stabilization. To assess whether the older memory could be strengthened, we repeated the triggering of reconsolidation. Strong young or old memories modulated by a threatening experience could be interfered during reconsolidation and updated (strengthened) by reconsolidation. Rather than being fixed, boundary conditions vary according to the memory features (strong memory), which indicates the dynamic nature of the reconsolidation process. Our findings demonstrate that it is possible to modifythese limits by recruiting the reconsolidation process and making it  functionally operative again. This novel scenario opens the possibility to new therapeutically approaches that take into account the reconsolidation process.","palabras_clave":"reconsolidation|boundary conditions|Strengthening function|declarative memory"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4341,"idioma_id":4,"titulo":"Biomimetic fiber mesh scaffolds based on gelatin and hydroxyapatite nano-rods: designing intrinsic skills to attain bone reparation abilities","resumen":"Intrinsic material skills have a deep effect on the mechanical and biological performance of bone sub-stitutes, as well as on its associated biodegradation properties. In this work we have manipulated thepreparation of collagenous derived fiber mesh frameworks to display a specific composition, morphology,open macroporosity, surface roughness and permeability characteristics. Next, the effect of the inducedphysicochemical attributes on the scaffold?s mechanical behavior, bone bonding potential and biodegrad-ability were evaluated. It was found that the scaffold microstructure, their inherent surface roughness,and the compression strength of the gelatin scaffolds can be modulated by the effect of the cross-linkingagent and, essentially, by mimicking the nano-scale size of hydroxyapatite in natural bone. A clear effectof bioactive hydroxyapatite nano-rods on the scaffolds skills can be appreciated and it is greater than theeffect of the cross-linking agent, offering a huge perspective for the upcoming progress of bone implanttechnology.","palabras_clave":"GELATIN|HYDROXYAPATITE|BONE|SCAFFOLDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4342,"idioma_id":4,"titulo":"Avalanche-like metamagnetic transition in (LaNd)CaMnO manganites","resumen":"We investigate the nature of ultrasharp metamagnetic transitions in La5\/8-yNdyCa3\/8MnO3 manganites.These compounds change from a low temperature ferromagnetic metallic state at low Nd doping to acharge-ordered antiferromagnetic insulator for high Nd content. At an intermediate doping a phaseseparatedstate is established. At low temperatures (~2 K), an avalanche-like field-induced metamagnetictransition is observed, where the entire compound changes abruptly from one phase to theother. The behavior of this ultrasharp transition is investigated using magnetization and specific heatmeasurements. We found a first order transition in the specific heat associated with discontinuous jumpsin the magnetization. A strong increase of the sample temperature is simultaneously observed. The resultsare interpreted in terms of latent heat released from the field-induced enhancement of theferromagnetic fraction, triggering the avalanche process.","palabras_clave":"MANGANITE|METAMAGNETIC|MAGNETOCALORIC|AVALANCHE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4344,"idioma_id":4,"titulo":"LAGRANGIAN REDUCTION OF DISCRETE MECHANICAL SYSTEMS BY STAGES","resumen":"In this work we introduce a category of discrete Lagrange{Poincaresystems LPd and study some of its properties. In particular, we show that thediscrete mechanical systems and the discrete dynamical systems obtained bythe Lagrangian reduction of symmetric discrete mechanical systems are objectsin LPd. We introduce a notion of symmetry group for objects of LPd as well asa reduction procedure that is closed in the category LPd. Furthermore, undersome conditions, we show that the reduction in two steps (rst by a closednormal subgroup of the symmetry group and then by the residual symmetrygroup) is isomorphic in LPd to the reduction by the full symmetry group.","palabras_clave":"MECÁNICA GEOMÉTRICA|SISTEMAS DINÁMICOS DISCRETOS|SIMETRÍAS|REDUCCION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4345,"idioma_id":4,"titulo":"First principles and experimental studies of the structural and magnetic ground state of the ternary compound MnFe3N","resumen":"In the present work, we report an extensive theoretical and experimental study in order to understood and determinate the structural and magnetic ground state of the MnFe3N. For such purpose, ab-initio calculations based on the Density Functional Density have been performed, considering the crystal structures Pm-3m (isostructural to g0-Fe4N) and P4\/mmm, and for each crystal structure differentmagnetic state have been regarded. Moreover, several experimental techniques such as X-ray diffraction, M?ossbauer spectroscopy at room temperature and magnetization measurement at 5 K and 100 K were carried out. From the theoretical side, the P4\/mmm crystal structure, where the magnetic moment of the atoms Mn and Fe has an antiparallel alignment, belong to ground state, and is supported by theexperimental results.","palabras_clave":"AB INITIO|ALLOYS|MNFE3N|MÖSSBAUER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4346,"idioma_id":5,"titulo":"ORDENAMIENTO Y CARACTERIZACIÓN FAUNÍSTICA DEL CRETACICOSUPERIOR DEL GRUPO CHUBUT, CUENCA DEL GOLFO  SAN JORGE, ARGENTINA","resumen":"O Grupo Chubut, Patagônia central, Argentina, é caracterizado por um sistema lacustre e fluvio-lacustre, com uma participação variável de cinza vulcânica. Este grupo inclui a Formação Bajo Barrel (Cenomaniano-Turoniano) e uma nova unidade litoestratigráfica recentemente depositada, a Formação Lago Colhué Huapi (Coniaciano-Maastrichtiano). A Formação Lago Colhué Huapi se sobrepõe à Bajo Barreal. Estas unidades sedimentares contém um rico e diversificado registro fóssil de vertebrados que inclui, entre outros, representantes de Crocodylomorpha, Testudines, Pterosauria e abundantes Dinosauria. Entretanto, as posições estratigráficas de diversos destes taxa são historicamente controversas. A origem estratigráfica pouco clara desses táxons, dificulta a interpretação correta das relações de seus fósseis com a fauna de outras bacias patagônicas e sul-americanas. Sendo assim, dentro deste contexto, nós apresentamos um posicionamento estratigráfico detalhado dos vertebrados fósseis das duas formações do Cretácio Superior, bem como discutimos as implicações destes arranjos faunísticos em termos de evolução dos vertebrados e paleobiogeografia. Por fim, o presente estudo amplia nosso conhecimento sobre a fauna fóssil destas unidades e também das assembléias de vertebrados da Patagônia central.","palabras_clave":"CHUBUT GROUP|BAJO BARREAL FORMATION|LAGO COLHUE HUAPI FORMATION|LATE CRETACEOUS|VERTEBRATE ASSEMBLAGES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4347,"idioma_id":5,"titulo":"Aplicaciones del cultivo celular en Odontología","resumen":"Las ciencias básicas, la medicina oral y los nuevos avances en biotecnología y bioinformática constituyen un gran campo de investigación dentro de la odontología actual. En este sentido, dichos avances están proporcionando un nuevo conjunto de estrategias terapéuticas para el manejo clínico de los pacientes con dolencias dentales y craneofaciales.Es importante destacar que las disciplinas relacionadas con las ciencias básicas, la medicina oral, la biotecnología y la bioinformática, han contribuido de manera trascendental al entendimiento de la fisiología y las diversas patologías que afectan las condiciones de normalidad del sistema bucal.La ingeniería tisular se considera como un enfoque prometedor para la odontología regenerativa, con el objetivo final de reemplazar morfológica y funcionalmente los tejidos periodontales y\/o los dientes perdidos a través de la síntesis in vitro de sustitutos análogos tisulares, considerando que el diente y las estructuras periodontales son importantes órganos del complejo craneofacial, los tratamientos utilizados para las enfermedades quelos afectan no lo restauran completamente.La odontología clínica está incursionando en una nueva era en donde el enfoque terapéutico es el uso de terapia génica, terapia celular, ingeniería tisular y la medicina regenerativa, ampliando el arsenal de posibilidades para nuestros pacientes. Una línea de investigación fundamental en ingeniería tisular y medicina regenerativa son las células madres. Como parte de los nuevos avances de la odontología a nivel mundial, científicos e investigadores del mundo aplican la bioingeniería para lograr reconstrucciones maxilofaciales, regeneraciones óseas y reconstrucciones de piezas dentales a partir de células madre como parte de tratamientos innovador.","palabras_clave":"CULTIVOS CELULARES|MEDIOS DE CULTIVO|INGENIERA TISULAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4348,"idioma_id":4,"titulo":"Ultrasonic compatibilization of polyelectrolyte complex based on polysaccharides for biomedical applications","resumen":"In recent years, there has been an increasing interest in the design of biomaterials for cartilage tissue engineering. This type of materials must meet several requirements. In this study, we apply ultrasound to prepare a compatibilized blend of polyelectrolyte complexes (PEC) based on carboxymethyl cellulose (CMC) and chitosan (CHI), in order to improve stability and mechanical properties through the inter-polymer macroradicals coupling produced by sonochemical reaction. We study the kinetic of the sonochemical degradation of each component in order to optimize the experimental conditions for PEC compatibilization. Scaffolds obtained applying this methodology and scaffolds without ultrasound processing were prepared and their morphology (by scanning electron microscopy), polyelectrolyte interactions (by FTIR), stability and mechanical properties were analyzed.  The swelling kinetics was studied and interpreted based on the structural differences between the two kinds of scaffolds. In addition we evaluate the possible in vitro cytotoxicity of the scaffolds using macrophage cells in culture. Our results demonstrate that the ultrasound is a very efficient methodology to compatibilize PEC, exhibiting improved properties compared with the simple mixture of the two polysaccharides. The test with murine macrophage RAW 264.7 cells showed no evince of cytotoxicity, suggesting that PEC biomaterials obtained under ultrasound conditions could be useful in the cartilage tissue engineering field.","palabras_clave":"ULTRASOUND|POLYELECTROLYTE COMPLEXE|SCAFFOLDS|CYTOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4349,"idioma_id":5,"titulo":"Análisis de una asignatura para la educación CTS: Biología, Genética y Sociedad","resumen":"La educación CTS ha logrado permear ampliamente tanto en el discurso de la didáctica de lasciencias como en documentos y lineamientos curriculares alrededor del mundo.Aún así, este tipo de contenido, si bien presente en las asignaturas de ciencia,se ha visto históricamente confinado y marginalizado en las seccionesintroductorias y menos comprometedoras del currículo. Por tal motivo, esteestudio se aboca al análisis de una de las nuevas asignaturas para la escuelasecundaria de la provincia de Buenos Aires (Argentina), llamada Biología,Genética y Sociedad,&nbsp; en busca de laimportancia que en su diseño curricular se le otorga al contenido CTS respectode los contenidos disciplinares más tradicionales. Lamateria propone el abordaje de una serie de estudios de casos distribuídos entres ejes, en donde las relaciones entre la ciencia, tecnología y sociedad sonineludibles. La variedad de temáticas CTS propuesta es tan amplia que nos parecióno sólo necesario evaluar esta presencia, sino también qué tipo de temáticasCTS son abordadas. Se utilizó una metodología mixta, que incluyó lacategorización y análisis de su diseño curricular; ese análisis cuantitativofue complementado con un análisis cualitativo: la realización de entrevistastanto a las autoras del diseño como a distintos funcionarios de la DirecciónGeneral de Cultura y Educación de la provincia. Se detectó una significativapresencia de contenidos CTS a lo largo de todo el diseño curricular, predominandolos de la sociología externa de la ciencia, por sobre los de la sociologíainterna y la epistemología.","palabras_clave":"Educación CTS|Escuela secundaria|Diseño curricular|Controversias sociocientíficas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4350,"idioma_id":5,"titulo":"Las mujeres y la Química","resumen":"Se implementó una actividad donde se estimula a los estudiantes a desempeñar un rol de comunicadores sociales a partir de profundizar su propia alfabetización científica. Tras la lectura del texto ?Las mujeres y la química: una complicidad de género? (Porro, 2011) en el cual se aborda cómo el desarrollo de la química ha afectado la vida de las mujeres y ha contribuido a cambiar el lugar de las mismas en la sociedad; las alumnas actuaron de transmisoras a personas alejadas de estos conocimientos. Se propuso a los estudiantes que eligieran algún tópico de los abordados en el texto y que conversaran con mujeres que no tuvieran conocimientos de química, explicándoles los nuevos conocimientos que estaban incorporando ellos mismos. La actividad tuvo muy buena aceptación y resulto gratificante para todos los actores involucrados. Actividades como la presentada en este trabajo permiten que los aspectos humanos y culturales de la ciencia y la tecnología sean más accesibles y relevantes para los estudiantes. La posibilidad de actuar como comunicadores sociales, no sólo revaloriza los conocimientos aprendidos, sino que también influye en el desarrollo de numerosas habilidades como individuos.","palabras_clave":"ALFABETIZACIÓN CIENTÍFICA|MUJERES Y QUÍMICA|COMUNICADORES SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4351,"idioma_id":4,"titulo":"On the strategies for incorporating nanosilica aqueous dispersion in the synthesis of waterborne polyurethane\/silica nanocomposites: effects on morphology and properties","resumen":"In this work the synthesis of waterborne polyurethane\/nanosilica nanocomposites by using two differentstrategies is presented, starting from a vinyl terminated polyurethane prepolymer (PUP) based onisophorone diisocyanate and polypropylene glycol, and varying the nanosilica content. In one case, thePUP was dispersed in an aqueous solution containing glycerol-functionalized colloidal nanosilica particlesand the dispersion was further polymerized; in the other case, the PUP was dispersed in an aqueousmedia, then colloidal nanosilica was added to the dispersion and then polymerized. A physical mixturewas also prepared for comparative purpose. Films were prepared by casting of the aqueous dispersion.The morphology of the dispersions and films depended on the incorporation route of nanoparticles aswas observed by SAXS, SEM and TEM. While the blends had significantly less hydrogen bonding betweenthe hard segments of the PU and nanosilica particles, samples prepared by the two different methodsproposed in this work showed an strong interaction between both materials in agreement with FTIR andDSC results. Synthesis pathway plays an important role in order to obtain high performance waterbornepolyurethane\/nanosilica composites, since final properties of the films also depended on the nanoparticleincorporation strategy.","palabras_clave":"NANOCOMPOSITES|POLYURETHANE\/NANOSILICA|WATERBORNE DISPERSIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4352,"idioma_id":4,"titulo":"Multivariate classification of honeys from Corrientes (Argentina) according to geographical origin based on physicochemical properties","resumen":"Honey is the natural sweet substance produced by Apis mellifera bees and is studied in different countries and zones because of the interest of consumers in origin and quality of food. The Corrientes Province (Argentina) produces and exports honey from different rural zones; therefore, the aim of this paper was to study the physico-chemical parameters of honeys using multivariate methods in order to classify honeys according to the geographical origin. Nine standard physico-chemical parameters were determined according to the international legislation. The results obtained were in agreement with international regulations. Then, the results obtained were analyzed by principal component analysis and linear discriminant analysis applying forward selection. The chemometric analysis allowed grouping samples according to their geographical origin by using only five parameters.","palabras_clave":"HONEY|GEOGRAPHICAL ORIGIN|CHEMOMETRICS|PCA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4353,"idioma_id":5,"titulo":"Presencia del inca en Yocavil (Catamarca, Argentina). Integración en la diversidad","resumen":"Se ofrece una síntesis sobre las particularidades de la presenciaincaica en el sur de Yocavil (Catamarca, Argentina), ponderando lascaracterísticas de las instalaciones imperiales, la alfarería incaica quecirculó en la región y la relevancia del QhapaqÑan como eje integrador. A los casos expuestos en síntesis previas se sumanueva información sobre instalaciones relevantes como El Calvario de FuerteQuemado y Punta de Balasto. Se reflexiona sobre las características de lasinteracciones entre las sociedades locales y el estado incaico en expansión, desde una óptica en la quese privilegia el estudio de los procesos de cambio a partir de la posición delas sociedades dominadas. Se concluye que la ocupación inca en el sur deYocavil fue intensa, con un reflejo arqueológico distintivo que no se ajustaestrictamente a los rasgos ?típicos? a partir de los cuales suele evaluarse lapresencia imperial en los Andes Meridionales.&nbsp;","palabras_clave":"YOCAVIL|RED VIAL INCAICA|ARQUITECTURA|ALFARERIA INCA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4354,"idioma_id":5,"titulo":"Avances en el tratamiento estadistico de datos gravimetricos de las sierras de san luis y comechingones. Importancia del grillado en procesamientos posteriores","resumen":"Based on gravimetric measurements made by IGSV (Instituto Geofísico Sismológico Volponi) and UNS (UniversidadNacional del Sur) we proceeded to the homogenization of the data in order to make Bouguer anomaly maps. Thesewill serve to analyze the cortical structures in the southern part of the Eastern Sierras Pampeanas of Argentina,between 31º and 33.5º S and 64º and 67.5º W, with a maximum resolution of approximately 2500 m.When working with higher derivatives we noted spurious results due to the Minimum Curvature (MC) method used forgridding the gravity data. This is undoubtedly a product of the irregular spacing of the initial data and a decay in theSignal to Noise Ratio (SNR) value. Therefore Kriging (KG) technique has been proposed and the results obtained bythis procedure have been compared with the previous MC applying differential operators such as total horizontalderivatives of the tilt angle and second derivatives.","palabras_clave":"GRAVIMETRÌA|BOUGUER|KRIGING|GRILLADO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4355,"idioma_id":4,"titulo":"A new brachyrostran with hypertrophied axial structures reveals an unexpected radiation of latest Cretaceous abelisaurids","resumen":"A well preserved skeleton of a new abelisaurid is reported here. The holotype of Viavenator exxoni was found in the outcrops of the Bajo de la Carpa Formation (Santonian, Upper Cretaceous), northwestern Patagonia, Argentina. This new taxon belongs to the South American clade of abelisaurids, the brachyrostrans. The current phylogenetic analysis places it as the basalmost member of a new clade of derived brachyrostrans, named Furileusauria, characterized by distinctive cranial, axial and appendicular anatomical features. The Santonian age of Viavenator allows filling the stratigraphic gap exhibited between the basal brachyrostrans of CenomanianeTuronian age, and the derived forms from the Campanian-Maastrichtian. The evolution of abelisaurids during the Late Cretaceous, faunal replacements, and the adaptive radiation that occurred during that period of time in South America are discussed.","palabras_clave":"Abelisauridae|Brachyrostra|Furileusaira|Bajo de la Carpa|Upper Cretaceous|Neuquén Basin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4356,"idioma_id":4,"titulo":"New record of ornithopod dinosaur from the Plottier Formation (Upper Cretaceous), Patagonia, Argentina","resumen":"Ornithopods are the least known dinosaurs within the Upper Cretaceous record of Argentina. For this reason everynewrecord is very important to know their evolution in South America. Here,we describe a new remain of an indeterminate ornithopod recovered in the Petrobrasaurus quarry of the Puesto Hernández area, northeastern Neuquén province (Argentina), late Coniacian?early Santonian in age. MAU-Pv-PH-458 is the northernmost bone record of an ornithopod in Argentina. This is a fragmentary neural arch from the middle section of the dorsal series of similar size to Macrogryphosaurus gondwanicus. MAU-Pv-PH-458 has typical ornithopod characters such as a lateromedial narrow neural spine and transverse processes dorsoposteriorly to posteriorly oriented. It shares with Macrogryphosaurus the presence of a deep channel between the bases of the postzygapophysis, which is a continuation of the channel that separates the postzygapophyses in posterior view. MAU-Pv-PH-458 increases the ornithopod record from the Plottier Formation.","palabras_clave":"Dinosauria|Ornithopoda|Cretaceous|Coniacian?Santonian"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4357,"idioma_id":4,"titulo":"EARLY CAMBRIAN U-Pb ZIRCON AGE AND Hf-ISOTOPE DATA FROM THE GUASAYÁN PLUTON, SIERRAS PAMPEANAS, ARGENTINA:  IMPLICATIONS FOR THE NORTHWESTERN BOUNDARY OF THE PAMPEAN ARC","resumen":"An Early Cambrian pluton, known as the Guasayán pluton, has been identified in the central area of Sierra de Guasayán, northwestern Argentina. A U?Pb zircon Concordia age of 533 ± 4 Ma was obtained by LA-MC-ICP-MS and represents the first report of robustly dated Early Cambrian magmatism for the northwestern Sierras Pampeanas. The pluton was emplaced in low-grade metasedimentary rocks and its magmatic assemblage consists of K-feldspar (phenocrysts) + plagioclase + quartz + biotite, with zircon, apatite, ilmenite, magnetite and monazite as accessory minerals. Geochemically, the granitic rock is a metaluminous subalkaline felsic granodiorite with SiO2 = 69.24%, Na2O+ K2O = 7.08%, CaO = 2.45%, Na2O\/ K2O = 0.71 and FeO\/MgO = 3.58%. Rare earth element patterns show moderate slope (LaN\/YbN = 8.05) with a slightly negative Eu anomalies (Eu\/Eu* = 0.76). We report the first in situ Hf isotopes data (&#949;Hft = -0.12 to -4.76) from crystallized zircons in the Early Cambrian granites of the Sierras Pampeanas, helping to constrain the magma source and enabling comparison with other Pampean granites. The Guasayán pluton might provide a link between Early Cambrian magmatism of the central Sierras Pampeanas and that of the Eastern Cordillera, contributing to define the western boundary of the Pampean paleo-arc.","palabras_clave":"U-Pb GEOCHRONOLOGY|Hf ISOTOPES|PAMPEAN GRANITES|SIERRA DE GUASAYÁN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4358,"idioma_id":4,"titulo":"Paleogeographic and paleoenviromental variations in the area of the Pueyrred´on, posadas and salitroso lakes, Santa Cruz Province, Argentina, during the Holocene and its relationship with the occupational dynamics","resumen":"This work constitutes an integration of geological, archaeological and palynological data from Santa Cruz Province, Argentina, for assessing the influence of paleogeographic and paleoclimatic changes in relation to the dynamics of humanoccupation during the Holocene. In order to carry out its objectives, we surveyed different archaeological sites, we collected pollen samples from two archaeological sites, and described, sampled, and dated stratigraphic profiles of lake levels. A digital elevation model was also developed to reconstruct old flooded areas. Given the obtained results, we observe the influence that paleogeographic changes couldhave had on human occupational dynamics. These changes may have opened upnew areas for both settlement and mobility. Pollen records suggest a vegetationchange from grass-steppe to shrub-steppe during the middle Holocene, most likelyrelated to climate changes identified at a regional scale.","palabras_clave":"PALEOGEOGRAPHIC  |PALEOENVIRONMENTAL VARIATION|POLLEN RECORDS|OCCUPATIONAL DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4359,"idioma_id":4,"titulo":"Placental programmed cell death: insights into the role of aquaporins","resumen":"STUDY HYPOTHESIS:Are the placental aquaporins (AQPs) involved in the apoptosis of human trophoblast?STUDY FINDING:The general blocking of placental AQPs with HgCl2 and, in particular, the blocking of AQP3 activity with CuSO4 abrogated the apoptotic events of human trophoblast cells.WHAT IS KNOWN ALREADY:Although apoptosis of trophoblast cells is a natural event involved in the normal development of the placenta, it is exacerbated in pathological processes, such as pre-eclampsia, where an abnormal expression and functionality of placental AQPs occur without alterations in the feto-maternal water flux. Furthermore, fluctuations in O2 tension are proposed to be a potent inducer of placental apoptotic changes and, in explants exposed to hypoxia\/reoxygenation (H\/R), transcellular water transport mediated by AQPs was undetectable. This suggests that AQPs might be involved in processes other than water transport, such as apoptosis.STUDY DESIGN, SAMPLES\/MATERIALS, METHODS:Explants from normal term placentas were maintained in culture under conditions of normoxia, hypoxia and H\/R. Cell viability was determined by assessing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide incorporation. For the general or specific inhibition of AQPs, 0.3 mM HgCl2, 5 mM CuSO4, 0.3 mM tetraethylammonium chloride (TEA) or 0.5 mM phloretin were added to the culture medium before explants were exposed to each treatment. Oxidative stress parameters and apoptotic indexes were evaluated in the presence or absence of AQPs blockers. AQP3 expression was confirmed by western blot and immunohistochemistry.MAIN RESULTS AND THE ROLE OF CHANCE:First, we observed that in H\/R treatments cell viability decreased by 20.16 ± 5.73% compared with those explants cultured in normoxia (P = 0.009; n = 7). Hypoxia did not modify cell viability significantly. Both hypoxia and H\/R conditions induced oxidative stress. Spontaneous chemiluminescence and thiobarbituric acid reactive substance levels were significantly increased in explants exposed to hypoxia (n = 6 per group, P = 0.0316 and P = 0.0009, respectively) and H\/R conditions (n = 6 per group, P = 0.0281 and P = 0.0001, respectively) compared with those cultured in normoxia. Regarding apoptosis, H\/R was a more potent inducer of trophoblast apoptosis than hypoxia alone. Bax expression and the number of apoptotic nuclei were significantly higher in explants cultured in H\/R compared with normoxia and hypoxia conditions (n = 12, P = 0.0135 and P = 0.001, respectively). DNA fragmentation was only observed in H\/R and, compared with normoxia and hypoxia, the activity of caspase-3 was highest in explants cultured in H\/R (n = 12, P = 0.0001). In explants exposed to H\/R, steric blocking of AQP activity with HgCl2 showed that DNA degradation was undetectable (n = 12, P = 0.001). Bax expression and caspase-3 activity were drastically reduced (n = 12, P = 0.0146 and P = 0.0001, respectively) compared with explants cultured in H\/R but not treated with HgCl2. Similar results were observed in explants exposed to H\/R when we blocked AQP3 activity with CuSO4. DNA degradation was undetectable and the number of apoptotic nuclei and caspase-3 activity were significantly decreased compared with explants cultured in H\/R but not treated with CuSO4 (n = 12, P = 0.001 and P = 0.0001, respectively). However, TEA and phloretin treatments, to block AQP1\/4 or AQP9, respectively, failed in abrogate apoptosis. In addition, we confirmed the expression and localization of AQP3 in explants exposed to H\/R.LIMITATIONS, REASONS FOR CAUTION:Our studies are limited by the number of experimental conditions tested, which do not fully capture the variability in oxygen levels, duration of exposure and alternating patterns of oxygen seen in vivo.WIDER IMPLICATIONS OF THE FINDINGS:Our results suggest that any alteration in placental AQP expression might disturb the equilibrium of the normal apoptotic events and may be an underlying cause in the pathophysiology of placental gestational disorders such as pre-eclampsia. Furthermore, the dysregulation of placental AQPs may be one of the crucial factors in triggering the clinical manifestations of pre-eclampsia.LARGE SCALE DATA:n\/a.STUDY FUNDING AND COMPETING INTERESTS:This study was supported by UBACyT 20020090200025 and 20020110200207 grants and PIP-CONICET 11220110100561 grant, and the authors have no conflict of interest to declare.","palabras_clave":"apoptosis|trophoblast|aquaporins|human placenta|preeclampsia|hypoxia intermittent"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4360,"idioma_id":4,"titulo":"A spatio-temporal comparative study of the representation of precipitation over South America derived by three gridded data sets","resumen":"In the last years there has been an increasing application of gridded precipitation datasets for studies analyzing seasonal and interannual precipitation variability as well as for performing validation analysis of climate and regional models. Consequently, the purpose of the present study is to analyze the representation of temporal variability and spatial patterns of precipitation in South America for the period 1971-2010, derived by three datasets based exclusively on rain gauge information (Climate Research Unit -CRU-, Global Precipitation Climatology Centre -GPCC, and University of Delaware -UDel-), in order to identify discrepancies and\/or agreements among them. Further analysis is performed for three particular sub-regions within the continent (Southeastern South America - SESA, Central South America-CSA and Southeast Brazil - SEBR). Results show that the three products represent the key spatial features of precipitation analogously. Significant coherence in capturing the temporal variability, associated with the dominant modes of variability, is found in all sub-regions, though they differ largely in some areas, especially in CSA. Overall, depending on the region, the gridded products behave differently, without one particular dataset standing out as an outlier for all the analyzed cases.","palabras_clave":"precipitation|gridded datasets|South America|comparison"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4361,"idioma_id":4,"titulo":"Viral evolution during chronic hepatitis B as revealed by ultra-deep sequencing data","resumen":"Recent studies indicate that viral diversity progressively increases along the course of chronic hepatitis B virus infection (CHB) and that some viral mutations correlate with liver disease severity. Using ultra deep sequencing (UDS) data, we detected such mutations at low frequencies among three immunotolerant patients and at high frequencies in an inactive carrier. None of these patients, the mother and children of one family, had severe liver injuries. Altogether, these data point out the potential of UDS in CHB monitoring. In addition, we performed in-depth evolutionary analyses which revealed that viral clearance seems not to require viral effective population size to decline. A detailed genetic analysis of the viral lineages arisen during and after the clearance suggested that mutations at or close to critical elements of the core promoter (enhancer II, epsilon encapsidation signal, TA2, TA3 and DR1-HRE) might be responsible for a sustained replication. This hypothesis requires viral load declination to be explained by a constant clearance of virus-producing hepatocyte, consistent with the sustained progress towards serious liver conditions experienced by many CHB patients.","palabras_clave":"HBV|pyrosequencing|high performance phylogenetics|virus evolution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4362,"idioma_id":4,"titulo":"State of the art of environmentally friendly sample preparation approaches for determination of PBDEs and metabolites in environmental and biological samples: A critical review","resumen":"Green chemistry principles for developing methodologies have gained attention in analytical chemistry in recent decades. A growing number of analytical techniques have been proposed for determination of organic persistent pollutants in environmental and biological samples. In this light, the current review aims to present state-of-the-art sample preparation approaches based on green analytical principles proposed for the determination of polybrominated diphenyl ethers (PBDEs) and metabolites (OH-PBDEs and MeO-PBDEs) in environmental and biological samples. Approaches to lower the solvent consumption and accelerate the extraction, such as pressurized liquid extraction, microwave-assisted extraction, and ultrasound-assisted extraction, are discussed in this review. Special attention is paid to miniaturized sample preparation methodologies and strategies proposed to reduce organic solvent consumption.Additionally, extraction techniques based on alternative solvents (surfactants, supercritical fluids, or ionic liquids) are also commented in this work, even though these are scarcely used for determination of PBDEs. In addition to liquid-based extraction techniques, solid-based analytical techniques are also addressed. The development of greener, faster and simpler sample preparation approaches has increased in recent years (2003e2013). Among green extraction techniques, those based on the liquid phase predominate over those based on the solid phase (71% vs. 29%, respectively). For solid samples, solvent assisted extraction techniques are preferred for leaching of PBDEs, and liquid phase microextraction techniques are mostly used for liquid samples. Likewise, green characteristics of the instrumental analysis used after the extraction and clean-up steps are briefly discussed.","palabras_clave":"PBDEs|METABOLITES|GREEN CHEMISTRY|REVIEW|SAMPLE PREPARATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4363,"idioma_id":4,"titulo":"First Report of Strawberry polerovirus 1 in Argentina","resumen":"Strawberry (Fragaria × ananassa Duch.) can be infected with multipleviruses in single or mixed infections producing severe symptoms andsignificant yield reduction. Among aphid-borne viruses, Strawberrymild yellow edge virus (SMYEV), Strawberry crinkle virus (SCV), andStrawberry mottle virus (SMoV) have been detected in Argentina.Strawberry plants with dwarfism, general weakness, distorted leaves,occasional chlorosis, few flowers, and poor fruit quality are observedoften in Argentina. The productivity of these affected plants is greatlyreduced. Symptomatic plants from Tucum´an, Argentina (26°559220 S;65°209150 W), were collected from the field and maintained in a greenhouse.Total RNA was extracted from leaves using an RNeasy Plant Mini Kit(QIAGEN, Valencia, CA, USA) according to manufacturer?s instructions,and subjected to next-generation sequencing (NGS) at the Genomics andBioinformatics Platform, INDEAR Inc., Rosario, Argentina, using Roche454 Genome Sequencer FLX+. The sequence reads were quality filteredand assembled into contigs using the Newbler Assembly v2.5.3 software(Roche). Contigs were analyzed using NCBI?s Open Reading Frame(ORF) Finder and BLAST. Two contigs showed high identity withPolerovirus genera. The first 624-nt contig (GenBank Accession No.KR094960) containing an ORF showed 99% identity with coat proteingene, and the second 847-nt contig 98% identity with the RNA-dependentRNA polymerase of Strawberry polerovirus 1 (KM233706.1 and KM233705.1)(Xiang et al. 2015). Based on the partial RNA-dependent RNA polymerasesequence obtained and Strawberry polerovirus 1 sequence at GenBank, fourspecific primers were designed: Polero47Fw (GAGTGTCACCACCCCTCACTAC) \/ Polero2Rv (AGGCCTACACCAGCATCTAACTCA) that amplify afragment of 897 nt, and Polero2Fw (CCAACTAGCCAAGATCCCATCTGAAG) \/ Polero40Rv (CGAGACCTCGACTTTGAAACCCA) that amplify afragment of 1030 nt, both from the polymerase cistron of the virus. Since thereare no commercial antisera available for Strawberry polerovirus 1, virus testingby reverse transcription polymerase chain reaction (RT-PCR) with the primerslisted above was carried out. Fresh leaf tissue samples were collectedindividually from 44 symptomatic strawberry plants and tested using RTPCR.PCR fragments of the expected size were amplified from 10 plantsconfirming the polerovirus presence. Two polerovirus positive strawberryplants, analyzed by RT-PCR using the primers designed, were used forpetiole-insert leaflet grafting to strawberry virus indicator, Fragaria vescavar. semperflorens (Duch.) ?Alpine?. SMYEV, SMoV, and SCV in the firstplant, and SMYEV and SMoV in the second plant were found in addition toStrawberry polerovirus 1 by RT-PCR using specific primers (Perotto et al.2014; Conci et al. 2009; Thompson et al. 2003; Thompson and Jelkmann2004). Symptoms of virus infection were observed 12 days postgrafting and20 days after grafting; all the viruses mentioned were detected by RT-PCR,including the polerovirus. This is the first report of Strawberry polerovirus 1in Argentina.","palabras_clave":"SIN PALABRAS CLAVE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4364,"idioma_id":5,"titulo":"XENOLITOS ULTRAMÁFICOS EN LAVAS MÁFICAS NEÓGENAS DE LA PUNA NORTE","resumen":"En este trabajo se describe por primera vez el hallazgo de xenolitos ultramáficos incluidos en andesitas basálticas y andesitasdel volcanismo monogenético neógeno de la Puna norte. Consisten en fragmentos angulosos de rocas de color verde a negro,de 1 mm a 5 cm de diámetro con textura mayormente adcumular. Corresponden a piroxenitas dado que están constituidos poruna asociación de clinopiroxeno, ortopiroxeno y subordinados porcentajes de olivino, además de espinelo rico en Cr (Cr# ~50) e inclusiones de sulfuros de Fe y Ni. En todos los casos, el número de magnesio [100MgO MOL\/(MgO+FeO)MOL] de los minerales es elevado (Mg# 81 a 87) y las composiciones se solapan con las observadas en los cristales que integran las lavas hospedantes.La aplicación de geotermobarómetros de dos piroxenos indica temperaturas de equilibrio de entre 969 y 1246 °C y presiones máximas de hasta 8 kbar. Se propone que estos xenolitos ultramáficos tienen un origen cumular, formados en reservorios profundos probablemente asociados a zonas calientes de acumulación de magmas primitivos en la corteza inferior a media. A raíz de la preservación del equilibrio entre los cristales de los xenolitos y los líquidos asociados, se interpreta que estos últimos debieron transportarse hasta la superficie de manera muy veloz desde dichos reservorios profundos.","palabras_clave":"XENOLITO|LAVA|MAFICA|PUNA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4365,"idioma_id":4,"titulo":"The Cerro Bitiche Andesitic Field: Petrological diversity and implications for magmatic evolution of mafic volcanic centers from the northern Puna.","resumen":"The Cerro Bitiche Andesitic Field (CBAF) is one ofthe two largest mafic volcanic fields in northern Puna (22?24°S) and is spatially and temporally associated with ignimbriteserupted from some central Andean Altiplano-Puna VolcanicComplex calderas. The CBAF comprises seven scoria conesand widespread high-K calcalkaline lava flows that cover anarea of 200 km2. Although all erupted rocks have a relativelynarrow chemical range (56?62 % SiO2, 3?6 % MgO), there isa broad diversity of mineral compositions and textures. Theleast evolved lavas (&#8764;58?61 % SiO2) are high-Mg andesiteswith scarce (<10 %) microphenocrysts of either olivine ororthopyroxene. The small compositional range and low phenocrystcontent indicate evolution controlled by low percentages(<10 %) of fractional crystallization of olivine andclinopyroxene of magmas similar to the least evolved rocksfrom the field, accompanied by assimilation during rapid ascentthrough the crust. Evolved andesites (&#8764;62 wt% SiO2), onthe other hand, are porphyritic rocks with plagioclase +orthopyroxene + biotite and ubiquitous phenocryst disequilibriumtextures. These magmas were likely stored in crustalreservoirs, where they experienced convection caused by maficmagma underplating, magma mixing, and\/or assimilation.Trace element and mineral compositions of CBAF lava provide evidence for complex evolution of distinct magma batches.","palabras_clave":"ANDESITES|NORTHERN PUNA|CENTRAL ANDES|NEOGENE|ALTIPLANO-PUNA VOLCANIC COMPLEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4366,"idioma_id":4,"titulo":"Synthesis of PtSn nanostructured catalysts supported over TiO2 and Ce-doped TiO2 particles for the electro-oxidation of ethanol","resumen":"PtSn\/TiO2 and PtSn\/Ce-doped TiO2 catalysts were synthesized and evaluated for ethanol electrooxidation in acid media. Titanium dioxide and Ce-doped TiO2 nanoparticleswere prepared by hydrothermal method followed by calcination at 923 K. Bimetallic PtSn catalysts supported on the oxide materials were synthesized by microwave assisted reduction in ethylene glycol (EG). The structural properties of the resulting materials were evaluated via TEM and XRD, and the compositions were assessed by EDX and ICPAES analysis. PtSn nanoparticles of about 3?4 nm were deposited on TiO2 and Ce?TiO2 particles. It was found that the catalyst composition is scarcely influenced by the cerium content in the mixed oxides while the electrochemical surface area per unit mass decreases upon the incorporation of Ce in the anatase lattice.The electrochemical tests pointed out that the electrocatalytic activity for ethanol oxidation decreases markedly as the Ce content increases. The results indicate that the presence of cerium in the titanium dioxide crystalline network induces local structural and electronic  modifications, thereby leading to a reduction of the crystallinity, surface conductivity and the amount of OH species adsorbed on the surfaceof the oxide support.","palabras_clave":"CE-DOPING|TIO2-SUPPORT|PTSN-NANOPARTICLES|ETHANOL-ELECTRO-OXIDATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4367,"idioma_id":4,"titulo":"A Proline Rich N-Terminal Region of the Dengue Virus NS3 Is Crucial for Infectious Particle Production","resumen":"Dengue virus is currently the most important insect-borne viral human pathogen. Viral nonstructural protein 3 (NS3) is a key component of the viral replication machinery that performs multiple functions during viral replication and participates in antiviral evasion. Using dengue virus infectious clones and reporter systems to dissect each step of the viral life cycle, we examined the requirements of different domains of NS3 on viral particle assembly. A thorough site-directed mutagenesis study based on solvent-accessible surface areas of NS3 revealed that, in addition to being essential for RNA replication, different domains of dengue virus NS3 are critically required for production of infectious viral particles. Unexpectedly, point mutations in the protease, interdomain linker, or helicase domain were sufficient to abolish infectious particle formation without affecting translation, polyprotein processing, or RNA replication. In particular, we identified a novel proline-rich N-terminal unstructured region of NS3 that contains several amino acid residues involved in infectious particle formation. We also showed a new role for the interdomain linker of NS3 in virion assembly. In conclusion, we present a comprehensive genetic map of novel NS3 determinants for viral particle assembly. Importantly, our results provide evidence of a central role of NS3 in the coordination of both dengue virus RNA replication and particle formation.","palabras_clave":"DENGUE VIRUS|HELICASE|PARTICLE PRODUCTION|FLAVIVIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4368,"idioma_id":5,"titulo":"MAGMATISMO BÁSICO JURÁSICO-CRETÁCICO EN LOS ANDES FUEGUINOS Y SU RELACIÓN CON LA CUENCA MARGINAL ROCAS VERDES","resumen":"Numerosos filones capa, diques y coladas básicas bordean las ofiolitas de la cuenca marginal Rocas Verdes, afectados por metamorfismoregional de bajo grado. Se distinguen tres variedades según su paragénesis primaria: lavas y filones de plagioclasa- piroxeno, lavas y filones de plagioclasa y filones con hornblenda. Las dos primeras se intercalan en la Formación Lemaire (Jurásico Superior) sin afectar unidades suprayacentes (Formaciones Yahgán y Beauvoir). Muchos filones de estas dos variedades presentan evidencias de emplazamiento en sedimentos no consolidados, indicando contemporaneidad con la Formación Lemaire y coladas basálticas. Las lavas de plagioclasa-piroxeno son semejantes químicamente con sus equivalentes intrusivos y serían co-genéticas. Las variedades de plagioclasa constituyen los bordes de algunos filones de plagioclasa-piroxeno, o bien filones independientes; su geoquímica sugiere que derivan de las variedades de plagioclasa-piroxeno por asimilación cortical. En base a la información de campo y geoquímica, el origen de las variedades de plagioclasa-piroxeno y plagioclasa es atribuido a las etapas iniciales del rifting, previo al desarrollo de la dorsal centro-oceánica, confirmando interpretaciones anteriores. En los sectores alejados del eje de la cuenca, las rocas básicas, excepto variedades con hornblenda, se emplazaron previamentea la depositación de la Formación Yahgán, que caracteriza la etapa más avanzada de la cuenca. Contrariamente, las diabasas con hornblenda cortan a la Formación Yahgán y tienen una petrografía y geoquímica distinta a las demás variedades. Todo indica que se trata de una unidad diferente y más moderna. Se adopta el nombre formacional de Gabro Puente Quemado para esta unidad.","palabras_clave":"Rocas básicas|Estratigrafía|Petrografía|Geoquímica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4369,"idioma_id":4,"titulo":"Evaluation of a coupling interface for solving fluid structure interaction problems","resumen":"This research work evaluates the performance of a Fluid Structure Interaction (FSI) solver, which is created using a generic interface to couple two independent software packages. The basic idea is to combine the advantages of the two independent codes to create a powerful FSI solver for two and three dimensional FSI analysis using the concept of modular programming. A detailed description about the implementation of an interface to couple a three-field system involved in the analysis is given, and this developed interface can be generalized to others codes. Since solving complex FSI problems is very time consuming, the focus of this work is placed on the performance of the coupled solver, for which a FSI benchmark will be solved on a computer cluster in order to measure speed up and efficiency.","palabras_clave":"Scalability|Fluid Structure Interaction|Partitioned Coupling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4371,"idioma_id":4,"titulo":"Small mammal remains recovered from two archaeological sites in northwestern Mendoza (Late Holocene, Argentina): Taxonomic composition, taphonomic issues and paleoenvironmental implications","resumen":"We present the results of the extensive analysis on the taxonomic composition, the taphonomic characteristics, and the possible paleoenvironmental implications of the small mammal assemblages in two archaeological sites located in northwestern Mendoza, Argentina: Vaquería Gruta 1 and Rincon de los Helados. Such sites have been radiocarbon dated for the Late Holocene. The taphonomic characteristics of these remains, which were accumulated in VQG1 and RH by raptor Strigiformes and Falconiformes birds respectively, were analyzed by means of digestive corrosion on the bones, breakage patterns and relative abundances. In order to evaluate the changes in the composition between past and current assemblages, zooarchaeological and current small mammal assemblages were compared. The taxonomic composition and abundance of small mammals in archaeological assemblages suggest the existence of greater environmental heterogeneity than the current during the Late Holocene, as would suggest the presence of Reithrodon auritus and Octomys mimax, currently extinct species in the region. The co-occurrence of these species currently with allopatric distributions, lead us to consider these archaeological sets as nonanaloguecommunities. In relation with the changes detected between past and current assemblages, some factors need to be considered. Among them, the relevance of the anthropic activities that have negatively affected the diversity, richness and abundance of flora and fauna since the XVI century, and the possible mixture of taxa due to the predation by owls in different altitudinal levels of vegetation.","palabras_clave":"Zooarchaeology|Microvertebrates|Pellet raptors|Local extinctions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4372,"idioma_id":4,"titulo":"ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF HYDROALCOHOLIC EXTRACTS FROM ALOYSIA POLYSTACHYA GRISEB MOLDENKE AND LIPPIA TURBINATA GRISEB (VERBENACEAE)","resumen":"Objective: The aims of this study were to determine polyphenols and flavonoids composition, to evaluate the antioxidant and antibacterial activities and possible antibacterial synergistic effects of hydroalcoholic extracts of Aloysia polystachya and Lippia turbinata.Methods: The flavonoids analysis was carried out in ethyl acetate fractions by means of high-performance liquid chromatography coupled to electrospray ionization quadrupole time of flight mass spectrometry (HPLC-ESI-Q-TOF-MS). The phenolic content was measured using Folin Ciocalteu reagent. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical method was used to estimate the antioxidant capacity. The antibacterial activity was determined by theResults: Several flavonoid compounds were detected for the first time in these species, being Lippia turbinata extract the one that presented the highest total phenolic content and antioxidant activity.agar microdilution and broth microdilution methods. The checkerboard assay was carried out to determine possible antibacterial synergism.Extracts from both species would have bactericidal activity against Staphylococcus strains employed in this work. The extract combinations had a synergistic effect against three bacteria, one of them Gram-negative.Conclusion: These extracts have demonstrated antioxidant and antibacterial activity, and its combination showed a synergistic effect against methicillin-resistant Staphylococcus aureus. Biological activities would be attributed to the whole of phytochemical constituents present in the respective crude extracts. However, it could be considered that the found flavonoids play an important role in the development of these activities.","palabras_clave":"Aloysia polystachya |Antibacterial activity| Antioxidant activity|Flavonoids|Lippia turbinata"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4373,"idioma_id":4,"titulo":"THE GEOCHEMICAL SIGNATURE OF SUSPENDED SEDIMENTS IN THE PARANA RIVER BASIN: IMPLICATIONS FOR PROVENANCE, WEATHERING AND SEDIMENTARY RECYCLING","resumen":"The Paraná River basin is one of the largest hydrological systems in South America. The present study focuses on the mineralogical and chemical composition of the suspended sediments exported by the Paraná River to the Atlantic Ocean, with the aim of analyzing their provenance, the chemical weathering signature and the likelihood of sedimentary recycling. The particulate matter of the Middle Paraná River and its main tributaries (i.e., the Paraguay and the Upper Paraná rivers) is mostly composed of quartz, plagioclase, K-feldspar, and clays, such as illite and kaolinite. Different geochemical approaches indicate that the suspended sediments transported by the Paraná River preserve the chemical signature of its sources and its composition is not significantly modified during transport. These sediments are mainly supplied by acidic arc sources located in the Andean headwaters of the Bermejo and Pilcomayo rivers (and transported by the Paraguay River); and by tholeiitic basalts outcropping in the headwaters of the Upper Paraná River. The incomplete mixing of both the main tributaries produces a transverse geochemistry geochemical asymmetry in the particulate material of the Middle Paraná River, which was detected ~32 km downflow the confluence. The suspended load transported by the Paraguay River (which includes the contributions from the Bermejo and Pilcomayo rivers) indicated indicates incipiently to moderately weathering, whereas the particulate matter exported by the Upper Paraná River reveals a higher degree of chemical alteration. This is the result of the different lithology and climatic regimens that prevail in the headwaters of both tributaries. The weathering signature of the Middle Paraná River's suspended load resembles that of the Paraguay River, which in turns supplies most of the particulate matter through the Bermejo River. This work also shows that the suspended load exported by the Paraná River basin has a mixed origin, where the chemical signatures from young materials derived from undifferentiated volcanic rocks, and from recycled materials affected by intracrustal differentiation can be distinguished","palabras_clave":"LARGE RIVERS|SUSPENDED LOAD|CHEMICAL ALTERATION|SEDIMENT SOURCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4374,"idioma_id":4,"titulo":"Contribution of resident and recruited macrophages to the photodynamic intervention of colorectal tumor microenvironment","resumen":"The study of cellular interactions in the tumor microenvironment has become one of the main areas of research in the fight against cancer. Tumor-associated macrophages (TAMs) influence tumor progression and therapy response due to its functional plasticity. Regarding cancer treatment, photodynamic therapy (PDT) is a minimally invasive and clinically approved procedure that involves the administration of a photosensitizer (PS), a nontoxic photosensitizing drug which is selectively retained in neoplastic tissue. Here, we investigated the role of resident and nonresident macrophages in the context of a PDT-treated colorectal tumor by developing a combination of 2-D and three-dimensional (3-D) experimental platform, recreating tumor-stroma interactions in vitro. Enhancement of cytotoxicity of PDT was achieved in the presence of nonresident macrophages which had a strong anti-tumor phenotype mediated by the production of nitric oxide, IL-6, and tumor necrosis factor alpha (TNF-&#945;). On the contrary, tumor resident macrophages induced a pro-tumor phenotype promoting tumor cell migration and endothelial stimulation. Due to their plasticity, tumor-resident or tumor-recruited macrophages can differentially influence the response of tumors to PDT, so their multifactorial roles should be considered in the overall design of anti-tumor therapeutic.","palabras_clave":"Photodynamic therapy | Tumor microenvironment |Macrophages | Colorectal cancer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4375,"idioma_id":4,"titulo":"Dendronization of chitosan films: surface characterization and biological activity","resumen":"An advanced modified chitosan film was designed and one-side-dendronized chitosan was obtained (ChPW).Undendronized chitosan films ChP and ChPWwere characterized in terms of their chemical structure, morphology,and biological properties. Atomic force microscopy (AFM) and optical microscopy analysis were used tocorroborate the topological changes of the modified dendronized side of the ChPW film. Both macrophages(RAW) and fibroblasts (NIH) were cultured and observed on three different extracellular matrices: ChP, ChPW,and cell culture plastic (control groups). ChP and ChPW showed noncytotoxicity toward RAW and NIH cells incontact with these films. The increased arginase activitywith ChPWand ChP films suggests an alternative profileof L-arginine metabolism in RAW, which favors cell proliferation and wound healing. The results indicate that theChPW scaffold is favorable for wound dressing in skin tissue engineering","palabras_clave":"chitosan|dendronization|macrophages|cytokines"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4376,"idioma_id":4,"titulo":"Inventory of primary emissions of selected persistent organic pollutants to the atmosphere in the area of Great Mendoza","resumen":"The setting up of a country or region-based inventory is considered a crucial step toward the elimination of worldwide persistent organic pollutants (POPs) contamination. Moreover, the need of comparable emission inventories at city or region level is widely recognized to develop evidence-based policies accounting for the relation between emissions and institutional, socio-economic and demographic characteristics at small scale level.Due to the low spatial and temporal resolution of the available measurements, highly variable air concentrations of several POPs have been observed in Latin American and Caribbean countries. This paper presents a high resolution spatially disaggregated atmospheric emission inventory for selected POPs in order to assess the environmental fate of some of these compounds in a finer resolution. As study case we estimated releases to air of POPs in a typical mid-size urban conglomeration in Argentina. Inventoried compounds were total polychlorinated biphenyls (PCBs), total polybrominated diphenyl ethers (PBDEs), total dichlorodiphenyltrichloroethane (DDT) on a sum basis, hexachlorobenzene (HCB) and dioxins and furans (PCDD\/Fs), for which emissions were estimated in 0.92 kg\/year, 1.65 kg\/year, 4.2E&#8722;02 kg\/year (total sum of congeners), 0.86 kg\/year and 4.4E&#8722;02 kg\/year respectively, values that are in accordance with the geographic and economic context. Although emitting sources are quite varied, there are very clear trends, particularly in relation to open burning of municipal solid waste and agrochemical use as major contributors. Overall, the inventory provides valuable data for the analysis of the heterogeneity of POP emissions and the necessary inputs for air quality modeling.","palabras_clave":"ATMOSPHERIC EMISSION INVENTORY|POPS|EMISSION FACTOR|OPEN BURNING MUNICIPAL SOLID WASTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4377,"idioma_id":4,"titulo":"Hydrological systems from the Antarctic Peninsula under climate change: James Ross archipelago as study case","resumen":"Multidisciplinary studies are described for several hydrological systems in the ice-free areas of theJames Ross archipelago (NE Antarctic Peninsula). This region provides a unique opportunity forstudying recent environmental changes that can be linked to recent climate change. Lake systemsand ephemeral ponds were developed on different periglacial environments, after ice cap retreatduring the Holocene. Geochemical, hydrological, sedimentological, and magnetic studies werecarried out to characterize and analyze different environments? behavior and dynamics. Moreover, asynthesis based on the observed diatom flora is made in the sampled habitats (lakes, streams andseepage areas, mosses). Based on an analysis of physicochemical characteristics, significantstatistical differences between lakes are observed. These differences can be attributed to differentprocesses (geochemical, diagenetic, biological, etc.) that could take place or particular characteristics of each lake?s catchments and environment (lithological, geomorphological).Marambio Island?s lakes present high dissolved concentrations (total dissolved solids ~3.300 mg L-1) due to the high rate of evaporation in this region, whereas their trace elements show differences inthe lithological source. Meanwhile lakes from Vega and James Ross islands are diluted waters withthe highest values of water?s pH (up to 10.2) as a result of biological photosynthesis. Within VegaIsland, trace elements discriminate sectors with statistical differences. The present study willcontribute to a better understanding of Antarctic lake systems from a multidisciplinary approach,and can be used as a baseline dataset of information.","palabras_clave":"water and sediments geochemistry|magnetic parameters|multivariate statistical|diatoms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4378,"idioma_id":4,"titulo":"Diagenetic analysis of tetrapod from the Upper Triassic, Puesto Viejo Group, Argentina","resumen":"The Puesto Viejo Group crops out in the San Rafael Block, southwest Mendoza, Argentina. This groupis an important unit for the knowledge theTriassic faunas. It is composed of basal Quebrada de los Fósiles Formation(QF) overlying by the Río Seco de la Quebrada Formation (RSQ). In this study, we report tetrapod remains from theRSQ Formation that includes, until now, exclusively therapsids. Non-mammalian therapsids predominated in terrestrialecosystems during the Late Paleozoic and Early Mesozoic. Although cynodonts and dicynodonts have been studied fromseveral perspectives, the relation between taxa and sedimentary context has been scarcely documented. This study isaimed to elucidate the diagenesis of tetrapods from the Puesto Viejo Group and its relation to depositional environment.Diagenetic features of therapsid bones from the RSQ Formation were analyzed through thin sections to interpret thedegree of taphonomic alteration. Diagenetic processes inferred include substitution; fracturing; brittle deformation anddifferent permineralization events. Combined analyses through scanning electron microscopy; energy-dispersive X-rayspectrometry (SEM-EDX); X-ray diffractometry (XRD) and petrographic studies reveals the substitution of hydroxyapatiteby francolite. The presence of fluorine in some samples suggests a link between the elemental composition and depositionalenvironments: floodplain and crevasse splay. Permineralization stages include infilling of vascular canals, trabeculae andfractures with iron oxides and iron carbonate minerals during the burial history. This contribution represents a relevantapproach to elucidate the relation between diagenesis on tetrapods and their paleoenvironments, as exemplified in thiscase study from the Puesto Viejo Group during the Upper Triassic.","palabras_clave":"DIAGENESIS|THERAPSIDS|UPPER TRIASSIC|PUESTO VIEJO GROUP, ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4379,"idioma_id":4,"titulo":"Volcanic ash from Puyehue-Cordón Caulle eruptions affects running performance and body condition of Phymaturus lizards in Patagonia, Argentina","resumen":"The Puyehue-Cordón Caulle eruption of 4 June 2011 dispersed about 100 million tonnes of pyroclastic materials resulting in ash accumulations of 30 cm depth on the Patagonian steppe, an area occupied by several lizard species. Herein we analysed, by experimental trials, the effects of ash and slope on running performance of two endemic and vulnerable species, Phymaturus excelsus and Phymaturus sinervoi, restricted to volcanic rock outcrops in Patagonia. We also determined the effect of ash fall on body condition by comparing the same populations before and after the volcanic eruption. Locomotion of P. excelsus, adapted to rocky and steep outcrops, was more affected in a negative way by ash. In contrast, P. sinervoi, which lives in mixed habitats with flat rocks and sandy substrates, ran more slowly on the inclined surface but was unaffected by ash, suggesting the two species are well adapted to the habitats they occupy. In spite of impacts of ash deposition on locomotion and potentially the feeding, reproduction and dispersal activity of P. excelsus, lizards captured 18 months after ash deposition showed improved body condition. Our study site for P. sinervoi received less ash deposition and hence body condition was similar before and after ash fall. We hypothesize that negative effects of ash on lizards were counteracted by competitive release; ash deposition caused an acute and significant increase in mortality of herbivorous competitors such as hares and sheep that feed upon the same flowers and fruits included in the Phymaturus diet.","palabras_clave":"body condition|disturbance|maximum running speed|Phymaturus excelsus|Phymaturus sinervoi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4380,"idioma_id":4,"titulo":"Weekly and monthly ground water recharge estimation in a rural piedmont environment using the water table fluctuation method","resumen":"La Colacha basin (Córdoba province, Argentina) is a typical piedmont rural area where the unconfined aquifer is used for agricultural activities. The objective of this work is to show the estimation of the recharge (R) rate in the unconfined aquifer, using the water table fluctuation method (WTF). Furthermore, considerations in relation to monthly and weekly recharge rhythms and to the aquifer discharge (D) were performed. The aquifer shows a typical behavior of groundwater recharge areas with an important and quick answer of water table to the arrival of precipitations (P). After that, a recession curve is observed, representing the groundwater discharge to the local base level (the main stream of the basin). The monthly estimation resulted in an annual average R value of 14.3 % of total P. Although the major amounts of recharge occur in full summer, according to the major total amounts of P, the correlation between monthly R and P was low (r2 < 0.2) as a result of the high quantities of rainfall water that are converted into runoff. The regression coefficient is higher (r2 = 0.6) for the end of summer and autumn when rainfalls diminish and have low intensities. This situation provides less water to the aquifer, but the recharge process is more efficient. The ratio R\/D for the 3 year series was positive, which means that the aquifer recharge was dominant. In the weekly recharge analysis, the annual average R is slightly lower than in the monthly one, that is, 12.4 % of the total P. Thus, it may be concluded that, in this case, the change from monthly to a weekly time step, did not much improve the final value. However, the information obtained with the weekly estimation is much more useful to interpret the aquifer detailed behavior.","palabras_clave":"recharge|time step|unconfined aquifer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4381,"idioma_id":4,"titulo":"Antioxidant Japanese plum (Prunus salicina) microparticles with potential for food preservation","resumen":"Fiber microparticles (MPCs) obtained from Japanese plum (Prunus salicina) skin and flesh through ethanolic extraction retained polyphenolic compounds, mainly proanthocyanidins, in 200-170 mg\/100gMPCs levels. Also, phenolics such as anthocyanins (cyanidin 3-galactoside and 3-rutinoside), responsible for red-purple colour, and flavonoids (quercetin derivatives), were found in significant proportions only in the skin MPCs. The MPCs-polyphenolic extract showed antioxidant capability (DPPH and FRAP assays) as well as protective effect against the oxidative stress induced by tert-butylhydroperoxide (t-BOOH) when biologically evaluated on kidney cells, joined to a low citotoxicity (50% cytotoxic concentration > 100 &#956;g\/mL). This is important since health benefits of barely absorbed proanthocyanidins rest on their permanence into the intestine, interfering with oxidative stress implicated in inflammatory disorders. Higher proportions of pectins found in flesh MPCs and of lignin in skin MPCs can provide different fibre functionality. Plum MPCs obtained can be useful as antioxidant natural additives and ingredients for functional food preservation.","palabras_clave":"PLUM FIBER MICROPARTICLES|PECTINS|FLAVONOIDS|PROANTHOCYANIDINS|OXIDATIVE STRESS|ANTIOXIDANT FOOD PRESERVATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4382,"idioma_id":4,"titulo":"In vitro and ex vitro germination of three Handroanthus species (Bignoniaceae)","resumen":"Handroanthus impetiginosus, H. ochraceus and H. lapacho, 'lapachos', are found in the Northwest of Argentina and have germination and conservation problems in their natural habitats. The germination of seeds under controlled conditions is an alternative that ensures the propagation. In this integrated study, in vitro and ex vitro germination, seed characteristics and seedling morphology of three species were analyzed. The length and width of the seeds, the width of the wings, the width and length of the seed body and embryo were measured. The germination capacity of the three species was determined during 12 months after seed harvesting, carrying out ex vitro and in vitro germination trials. The seeds and embryos of H. impetiginosus were significantly larger than those of other species. Handroanthus impetiginosus seeds maintained a high germination capacity over the 12 months (95%). Seed germination capacity of H. ochraceus gradually decreased four months after seed collection (63 to 47%), whereas that of H. lapacho decreased abruptly after eight months. No significant differences were observed between ex vitro and in vitro methods in H. impetiginosus and H. ochraceus. In the three species, the germination capacity is directly proportional to the size of seeds and seedlings.","palabras_clave":"semillas|lapacho|cultivo|morfologia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4383,"idioma_id":4,"titulo":"Geochemistry of a Triassic dyke swarm in the North Patagonian Massif, Argentina. Implications for a post-orogenic event of the Permian Gondwanide Orogeny.","resumen":"Permo-Triassic magmatism is widespread in the eastern North Patagonian Massif and has been related to the Gondwanide orogeny. Although a magmatic arc setting is widely accepted for the Permian plutonic rocks, the origin and geotectonic setting for the Triassic plutonic and volcanic rocks are still unknown. A NW-SE Triassic dyke swarm composed of andesites and latites with minor rhyolites was previously described in the Sierra Grande ? Rincon de Paileman area. The dyke swarm was associated with extensional tectonics which was linked to a postorogenic process. In this paper we present new geochemical data of the rocks that form the swarm. Trachyandesites and rhyolites were separated based on their geochemical characteristics. Both groups may be considered originated from different sources. On the other hand, the content of incompatible elements (LILE and HFSE) indicates a strong relation between the swarm and an active continental margin. The samples also show a transitional signature between continental-arc and postcollisional or anorogenic settings. The new geochemical data on the dyke swarm support the idea of a magmatism that was linked to a postorogenic extensional tectonic regime related to a continental magmatic arc. Such an extension started in the Paleopacific margin of Pangea during the Anisian and might indicate the beginning of the Pangea break-up.","palabras_clave":"PATAGONIA|TRACHYANDESITE|MILDY ALKALINE|EXTENSION|POSTOROGENIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4384,"idioma_id":4,"titulo":"Paleogene Glyptodontidae Propalaehoplophorinae (Mammalia, Xenarthra) in extra-Patagonian areas","resumen":"ABSTRACT. Paleogene records of Cingulata Glyptodontidae are scarce. The only well described comes from the Paleogene of Argentine Patagonia. Two subfamilies have been reported for that period: Glyptatelinae and Propalaehoplophorinae. Until this contribution, the latter taxon was geographically restricted to the locality of El Pajarito (Late Oligocene, Deseadan SALMA), Chubut province, Argentina. Here we present and describe the northernmost record of a Paleogene Propalaehoplophorinae. The material is represented by three associated osteoderms of the dorsal carapace from the Fray Bentos Formation (Late Oligocene, Deseadan SALMA) in the locality of Cueva del Tigre, Chajarí, Entre Ríos province, Argentina. Morphologically, these remains are almost identical to those reported from the late Oligocene of the Patagonian region, showing that during the Paleogene the Propalaehoplophorinae had a larger latitudinal distribution than previously known.","palabras_clave":"DESEADAN|OLIGOCENE|XENARTHRA|ENTRE RIOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4385,"idioma_id":4,"titulo":"Soil Physical Quality and Soybean Yield as Affected by Chiseling and Subsoiling of a No-till Soil","resumen":"The concept of soil physical quality (SPQ) is currently under discussion, and an agreement about which soil physical properties should be included in the SPQ characterization has not been reached. The objectives of this study were to evaluate the ability of SPQ indicators based on static and dynamic soil properties to assess the effects of two loosening treatments (chisel plowing to 0.20 m [ChT] and subsoiling to 0.35 m [DL]) on a soil under NT and to compare the performance of static- and dynamic-based SPQ indicators to define soil proper soil conditions for soybean yield. Soil sampling and field determinations were carried out after crop harvest. Soil water retention curve was determined using a tension table, and field infiltration was measured using a tension disc infiltrometer. Most dynamic SPQ indicators (field saturated hydraulic conductivity, K0, effective macroporosity, &#949;ma, total connectivity and macroporosity indexes [CwTP and Cwmac]) were affected by the studied treatments, and were greater for DL compared to NT and ChT (K0 values were 2.17, 2.55, and 4.37 cm h-1 for NT, ChT, and DL, respectively). However, static SPQ indicators (calculated from the water retention curve) were not capable of distinguishing effects among treatments. Crop yield was significantly lower for the DL treatment (NT: 2,400 kg ha-1; ChT: 2,358 kg ha-1; and DL: 2,105 kg ha 1), in agreement with significantly higher values of the dynamic SPQ indicators, K0, &#949;ma, CwTP, and Cwmac, in this treatment. The results support the idea that SPQ indicators based on static properties are not capable of distinguishing tillage effects and predicting crop yield, whereas dynamic SPQ indicators are useful for distinguishing tillage effects and can explain differences in crop yield when used together with information on weather conditions. However, future studies, monitoring years with different weather conditions, would be useful for increasing knowledge on this topic.","palabras_clave":"Effective porosity|water retention curve|soil loosening|pore connectivity index"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4386,"idioma_id":4,"titulo":"Scale dependency of Liolaemus lizards home range in response to different environmental variables","resumen":"Animal habitat-use patterns cannot be isolated from scale issues. Consequently, multi-scale studies provide a complete characterization of ecological patterns that can further explain the observed variation. Liolaemus constitutes the world´s second most speciose lizard genus. In this study, we assessed the relationships between home range size and environmental variables at 3 different spatial scales. The study at a local and regional scale was focused on the habitat specialist Liolaemus multimaculatus. The lizard´s home range was calculated using the minimum convex polygon method in populations from grassland sites of the coastal sand dunes of the Argentinean Pampas under 2 different conditions, with or without forestations of Acacia longifolia. On the other hand, at a geographical scale we considered the evolutionary implications of 20 species of Liolaemus. Home range size, phylogeny, ecological, environmental, and climatic data were obtained from the literature and remote sensing. L. multimaculatus home range varied from 12.66 to 570.00 m. Regionally, this species had smaller home ranges in forested habitats (X: 94.02m2) compared with the non-forested sites (X: 219.78m2). Habitat structure, vegetation types, and food availability would explain the space use at finer scales. When the 20 species of Liolaemus were considered, high mean air temperature and broad thermal amplitudes showed an inverse relationship with home range size. Neither net primary productivity nor phylogeny was good predictors for home range variation at geographical scale. This study highlights the scale dependence of the explicative capability of a set of environmental and intrinsic variables on home range patterns.","palabras_clave":"Home range|Environmental factors|Liolaemus|Lizard|Scales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4387,"idioma_id":4,"titulo":"Freshly characterization and storability of mini head lettuces at optimal and abusive temperatures","resumen":"Selection of lettuce varieties less sensitive to quality deterioration and more tolerant to abusive temperatures during handling, transportation, and storage is essential to minimize economical and quality losses that affect both producers and consumers. This work was focused on the quality changes of four baby head lettuces (Lactuca sativa L.), two butter (red and green) and two oak-leaf (red and green) types, during storage at 0 _C and 10 _C for 10 days. Lettuce quality was determined by measuring bioactive content (ascorbic acid, total phenolics), physicochemical (total chlorophyll, browning potential), and microbiological indices. At harvest, red varieties presented lower browning potential and higher bioactive compounds but no differences were observed in microbial populations. During storage, ascorbic acid underwent first order degradation for all varieties, with a degradation rate at 10 _C twice faster than at 0 _C. At 0 _C, only the red oak-leaf lettuce exhibited chlorophyll degradation, while at 10 _C all varieties presented degradation. No changes were observed in total phenolics and browning potential of butter lettuces during storage at both temperatures. Microbial population counts were significant affected by the storage temperature. Red butter baby lettuce presented slightly better bioactive content and microbiological characteristics and then better storability.","palabras_clave":"PHENOLICS|CHLOROPHYLLS|COLD STORAGE|FOOD QUALITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4388,"idioma_id":4,"titulo":"Enhancing quality attributes of fiber-enriched strawberry juice by application of vanillin or geraniol","resumen":"In this work, the impact of adding prebiotic fibers (inulin, oligofructose and apple fiber) to strawberry juices on quality properties was investigated. Additionally, the beneficial effects of natural preservatives (vanillin and geraniol) on quality parameters of fiber-enriched juices were studied. Microbiological, physicochemical and sensorial properties were evaluated throughout two weeks of storage at 5 °C. Results showed that the addition of prebiotic fibers significantly increased antioxidant capacity and successfully maintained sensory quality of strawberry juice when compared to fresh control. Vanillin and geraniol treatments had a marked effect in reducing native microflora counts (4?6 log cycles reductions) in comparison to untreated samples. Natural preservatives imparted strong flavor in fiber-enriched strawberry juices, however, it was pleasant for those samples treated with vanillin. Besides, overall visual quality scores in treated samples were maintained in the acceptability level throughout storage. Juices with vanillin showed the highest total phenolic content, indicating high nutritional value of the product. Nevertheless, both preservatives agents exhibited lower antioxidant activity than controls. Thus, prebiotic fibers could enhance nutritional and sensory quality of strawberry juice. Also, geraniol and vanillin could be feasible alternatives to improve microbiological quality with low impact on the organoleptic properties of fiber-enriched strawberry juice.","palabras_clave":"DIETARY FIBERS|NATURAL PRESERVATIVES|QUALITY PARAMETERS|STRAWBERRY JUICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4389,"idioma_id":5,"titulo":"Anatomía vegetativa de Lophophytum mirabile subsp. bolivianum (Balanophoraceae) y el efecto de su parasitismo en la anatomía de las raíces de su hospedante Anadenanthera colubrina","resumen":"The objectives of this work were to study the structure of the vegetative body of Lophophytum mirabile subsp. bolivianum; analyze the change on roots of Anadenanthera colubrina when they are infected by this parasitic plant, and identify the anatomical changes produced by that parasitism. Lophophytum mirabile plants are formed by a spheroidal- narrower underground vegetative body or tuber, externally has a dark warty surface, lacks epidermis, stomata or trichomes. The central matrix of tuber consists of reserving parenchyma and vascular bundles. Parasitic cells located at the level of root cambium initiates the tubers formation, externally visible as exophyte . On the infected root of A. colubrina, the identity of radial and axial growth of the secondary system it is lost. This leads to the formation of xylem loops that affecting the xylem transport and root development, which stops growing in length and develops a woody gall. Infection of L. mirabile causes profound anatomical changes in developing timber of A. colubrina, which favor the successful of the parasite.","palabras_clave":"ANATOMY|ROOT|LOG|PARASITISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4390,"idioma_id":4,"titulo":"Optimization of ultrasound, vanillin and pomegranate extract treatment for shelf-stable unpasteurized strawberry juice","resumen":"Optimum combination of ultrasound, vanillin and pomegranate extract to improve quality of strawberry juice was determined using response surface methodology. Samples were stored at 5 °C for 14 days. The optimal conditions to simultaneously minimize native microflora, maximize nutritional parameters and minimize the impact on sensory quality resulted in: 7.5 min of ultrasound treatment, pomegranate extract concentration of 360 &#956;g\/mL and vanillin concentration of 0.925 mg\/mL. A new batch of strawberry juice was treated at these optimal conditions and stored for validation of the optimization and to evaluate the performance of the optimum treatment on quality parameters throughout storage. Furthermore, a second batch of juice was inoculated with Escherichia coli O157:H7 and processed at optimal conditions to evaluate the effectiveness of the treatment on the pathogen survival. The native microflora of the juice, as well as inoculated pathogen, decreased significantly using the proposed hurdle technologies, with no impact on sensory parameters. Ascorbic acid retention was slightly decreased by the optimum treatment; however, DPPH and polyphenolic compounds were significantly higher than those in untreated sample. Overall, a combination of ultrasound, vanillin and pomegranate extract showed interesting potential to enhance quality and safety of strawberry juice, extending the shelf-life of the product","palabras_clave":"BIOPRESERVATIVES; |SIMULTANEOUS OPTIMIZATION; |HURDLE TECHNOLOGY; |UNPASTEURIZED JUICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4391,"idioma_id":4,"titulo":"Eugenol-loaded LLDPE films with antioxidant activity by supercritical carbon dioxide impregnation","resumen":"In this work, linear low density polyethylene (LLDPE) films were impregnated with eugenol using supercritical CO2 impregnation, as a strategy for obtaining a loaded material to be potentially applied in active food packaging. Eugenol, the main component of clove oil, is well known as a natural antioxidant and antimicrobial agent. Impregnation runs were performed in a lab-scale high pressure stirred cell at 45 °C during 4 h, and the effect of pressure (10, 12 and 15 MPa) and depressurization rate (0.5, 1 and 5 MPa\/min) on impregnation yield was evaluated. Yields of 1-6% (w\/w) were achieved, with higher values at low depressurization rate. Film samples were characterized by infra-red spectroscopy (FTIR) and differential scanning calorimetry (DSC). FTIR analysis suggested that the distribution of eugenol in the films is not totally homogeneous. DSC revealed a decrease in the crystallinity degree in the impregnated samples as well as in films subjected to pressurization with CO2 but not loaded with eugenol. Changes in mechanical properties were also investigated in a texturometer. A decrease of LLDPE Young modulus and ductility was observed after high pressure impregnation, although tensile strength was not significantly affected. Finally, the antioxidant activity of the eugenol-loaded films was assessed via the DPPH inhibition test.","palabras_clave":"SUPERCRITICAL FLUID IMPREGNATION|EUGENOL|LLDPE|ANTIOXIDANT ACTIVITY|ACTIVE PACKAGING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4392,"idioma_id":4,"titulo":"Alkaloids from Chelidonium majus L.: Fractionated supercritical CO2 extraction with co-solvents","resumen":"Chelidonium majus L. is rich in many isoquinoline alkaloids which are responsible for the anti-inflammatory, antitumor and cytotoxic bioactivities known for this plant. The main alkaloids identified in C. majus L. include chelidonine, berberine, coptisine, sanguinarine and chelerythrine, which are present in different parts of the plant. In this work, alkaloids from C. majus aerial and terrestrial parts were extracted using a two step extraction procedure which consisted in a first step (SFE) using only supercritical carbon dioxide (scCO2) as solvent followed by a second step (ESE) using scCO2 and a co-solvent mixture composed by an alcohol (ethanol or isopropanol) and diethylamine (alkaline conditions). The effect of operation temperature, pressure, solvent density and solvent pH on extraction yields, kinetic profiles and alkaloids? selectivity was evaluated. Results showed that SFE presents high selectivity for alkaloids (particularly for chelidonine) at solvent densities in the range 813-850 kg\/m3 and for both the aerial and terrestrial parts of the plant. The highest lipoxygenase inhibition capacity was observed for extracts obtained by SFE, in particular those obtained from the aerial parts of C. majus L. at higher solvent density conditions. The highest alkaloids extraction yield was observed at higher solvent density conditions using the scCO2\/isopropanol\/diethylamine mixture (ESE extraction step). The highest antioxidant capacity was observed for the extracts obtained from the terrestrial parts of C. majus L. using this co-solvent mixture at lower solvent density. Chelidonine was found to be highly soluble in scCO2. Therefore, the fractioned high pressure extraction procedure proposed in this work can be successfully applied to separate enriched chelidonine fractions, while other alkaloids can be obtained using basified isopropanol as co-solvent. This procedure demonstrates that alkaloids fractionation during extraction is an important tool to be used before further purification\/isolation steps.","palabras_clave":"CHELIDONIUM MAJUS|HIGH PRESSURE EXTRACTION|QUATERNARY ALKALOIDS|ANTI-INFLAMMATORY ACTIVITY|ANTIOXIDANT ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4393,"idioma_id":4,"titulo":"Mechanism of action of endosulfan as disruptor of gonadal steroidogenesis in the cichlid fish Cichlasoma dimerus","resumen":"The organochlorine pesticide endosulfan (ES) is used in several countries as awide spectrum insecticide on crops with high commercial value. Due to its high toxicity to non-target animals, its persistence in the environment and its ability to act as an endocrine disrupting compound in fish, ES use is currently banned or restricted in many other countries. Previous studies on the cichlid fish Cichlasoma dimerus have shown that waterborne exposure to ES can lead to both decreased pituitary FSH content and histological alterations of testes. As gonadotropin stimulated sex steroids release from gonads was inhibited by ES in vitro, the aim of the present study was to elucidate possible mechanisms of disruption of ES on gonadal steroidogenesis in C. dimerus, as well as compare the action of the active ingredient (AI) with that of currently used commercial formulations (CF). Testis and ovary fragments were incubated with ES (AI or CF) and\/or steroidogenesis activators or precursors. Testosterone and estradiol levels were measured in the incubation media. By itself, ES did not affect hormone levels. Co-incubation with LH and the adenylate cyclase activator forskolin caused a decrease of the stimulated sex steroids release. When co-incubated with precursors dehydroandrostenedione and 17&#945;hydroxyprogesterone, ES did not affect the increase caused by their addition alone. No differences were observed between the AI andCFs, suggesting that the effect on steroidogenesis disruption is mainly caused by the AI. Results indicate that action of ES takes place downstream of LH-receptor activation and upstream of the studied steroidogenic enzymes","palabras_clave":"endocrine disruption|gonad culture|organochlorine pesticide|commercial 40 formulation|active ingredients"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4394,"idioma_id":4,"titulo":"Effects of atrazine on growth and sex differentiation, in juveniles of the freshwater crayfish Cherax quadricarinatus","resumen":"The effect of the herbicide atrazine was assayed in early juveniles ofthe redclaw crayfish Cheraxquadricarinatus. Four cohorts of juveniles (a total of 280 animals) wereexposed for 4 wk to each one of three atrazine concentrations (0.1, 0.5 and 2.5mg\/L) or a control (0 mg\/L), from a commercial formulation having 90% of activeprinciple. At the end of the exposure, no significant (p&gt;0.05) differencesin either mortality or molting were noted. However, the weight gain and theprotein content of abdominal muscle decreased significantly (p&lt;0.05) in thehighest atrazine concentration as compared to control, indicating that atrazineacted as a relevant stressor, although at a concentration higher than thosereported in the environment. Besides, the proportion of females increasedprogressively as the atrazine concentration increases, being significantly(p&lt;0.05) higher than that of controls at the highest concentration assayed.Both macroscopic and histological analysis revealed a normal architecture ofgonopores and gonads in both control and exposed animals. The obtained resultsstrongly suggest that atrazine could be causing an endocrine disruption on thehormonal system responsible for the sexual differentiation of the studiedspecies, increasing the proportion of female proportion without disturbing thegonad structure.","palabras_clave":"Atrazine|Crayfish|Sex differentiation|Growth"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4395,"idioma_id":4,"titulo":"Electrochemical magnetic microbeads-based biosensor for point-of-care serodiagnosis of infectious diseases","resumen":"Access to appropriate diagnostic tools is an essential component in the evaluation and improvement of global health. Additionally, timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods such as culturing, enzyme linked immunosorbent assay (ELISA) or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments making them not adaptable to point-of-care (PoC) needs at resource-constrained places and primary care settings. Therefore, there is an unmet need to develop portable, simple, rapid, and accurate methods for PoC detection of infections. Here, we present the development and validation of a portable, robust and inexpensive electrochemical magnetic microbeads-based biosensor (EMBIA) platform for PoC serodiagnosis of infectious diseases caused by different types of microorganisms (parasitic protozoa, bacteria and viruses). We demonstrate the potential use of the EMBIA platform for in situ diagnosis of human (Chagas disease and human brucellosis) and animal (bovine brucellosis and foot-and-mouth disease) infections clearly differentiating infected from non-infected individuals or animals. For Chagas disease, a more extensive validation of the test was performed showing that the EMBIA platform displayed an excellent diagnostic performance almost indistinguishable, in terms of specificity and sensitivity, from a fluorescent immunomagnetic assay and the conventional ELISA using the same combination of antigens. This platform technology could potentially be applicable to diagnose other infectious and non-infectious diseases as well as detection and\/or quantification of biomarkers at the POC and primary care settings.","palabras_clave":"Point-of-Care diagnosis|Electrochemical biosensor|Magnetic-beads|Recombinant antigens|Infectious diseases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4396,"idioma_id":5,"titulo":"Efecto nematicida de extractos de ajo, orujo de uva y alperujo de aceituna; sobre Meloidogyne incognita, en vid, cv Chardonnay","resumen":"Meloidogyne incognita afecta significativamente el cultivo de vid en Argentina. Actualmente se dispone de pocas herramientas para su control. El objetivo de este trabajo fue comparar el efecto de extractos acuosos de bulbillos de ajo, de compost inmaduro de alperujo de aceituna, de compost inmaduro de orujo de uva fresco y de compost inmaduro de orujo de uva agotado, sobre el índice de agallamiento (IA), el índice de reproducción de huevos y juveniles J2 IR  la oblación final de hembras de M. incognita (H), en plantas de vid, cv Chardonnay. La experiencia fue realizada en invernáculo, con lanas en macea l nico raamieno e resenó dierencias sinificaias reseco del control fue el extracto de bulbillos de ajo; mientras que los extractos de compost inmaduro de alperujo de aceituna, de compost inmaduro de orujo de uva fresco y de compost inmaduro de orujo de uva agotado, no afectaron los parámetros evaluados. El extracto de bulbillos de ajo disminuyó un 73% el IA, un 80% el IR y un 94% de H, lo que permite esperar que en el futuro pueda desarrollarse a partir de este material vegetal, un nematicida orgánico y de bajo impacto ambiental.","palabras_clave":"PROTECCIÓN VEGETAL|VID|MELOIDOGYNE INCOGNITA |EXTRACTO VEGETAL|NEMATICIDA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4397,"idioma_id":4,"titulo":"Electroosmotic  flow profile distortion due to Laplace pressures at the end reservoirs","resumen":"The build-up of pressure-driven backflows is a known drawback to electroosmotic plug  flow. We have studied the characteristic time constant of these pressure gradients build-up as a function of various geometrical variables of the problem through &#956;PIV measurements. Previous models based on hydrostatic pressure difference (syphoning) cannot explain the dynamics of our experimental results. We have developed a model that relates these pressure-driven backflows to Laplace pressures, due to changes in the interface radii of curvature at the reservoirs. This model leads to an equation which was solved both numerically and, after linearisation, analytically. The characteristic time constants obtained show much better agreement with experimental data. Therefore, this model allows for predicting the time during which an acceptable electroosmotic  flow profile will last for a given microchannel?reservoir design.","palabras_clave":"MICROFLUIDICA|FLUJO ELECTROOSMOTICO|PRESIONES DE LAPLACE|ELECTROCINÉTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4398,"idioma_id":4,"titulo":"An Evolutionary Algorithm Applied to Inventory Control for Natural Gasoline","resumen":"A metaheuristic technique for controller design is explored and its performance is assessed for a real-world case. An evolutionary algorithm for self-tuning systems is proposed to tune the optimal parameter settings in order to achieve the best overall performance to control accurately the volume of natural gasoline in a storage tank. For high-level optimization a flexible iterative technique based on Genetic Algorithms is presented. The algorithm is stable and its architecture is simple, thus becoming easy to implement. Not only heuristics that produce superior solutions are taken into account, but also the computational speed is contemplated as a key factor. The experimental evaluation yields satisfactory fitness values and relatively little computational effort is required.","palabras_clave":"INVENTORY CONTROL|ON-LINE|GENETIC ALGORITHMS|GASOLINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4399,"idioma_id":4,"titulo":"Regulatory parameters of the lung immune response during the early phase of experimental trichinellosis","resumen":"Parasitic infection caused by Trichinella spiralis provokes an early stimulation of the mucosal immune system which causes an allergic inflammatory response in the lungs. The present work was intended to characterize the kinetics of emergence of regulatory parameters in Wistar rat lungs during this early inflammatory response, between days 0 and 13p.i. The presence of regulatory cells such as regulatory T cells (Tregs) and alternatively activated macrophages (AAM) was analyzed in lung cell suspensions. Moreover, a regulatory cytokine (TGF-&#946;) was studied in lung tissue extracts. Considering that newborn larvae (NBL) travel along the pulmonary microvasculature, the ability of this parasite stage to modulate the activation of lung macrophages was evaluated. For this purpose, lung macrophages from non-infected or infected rats (day 6p.i.) were cultured with live or dead NBL. Arginase activity (characteristic of AAM) and nitric oxide (NO produced by iNOS, characteristic of classical activated macrophages) were measured after 48h. Our results revealed a significant increase in the percentage of Tregs on days 6 and 13p.i., arginase activity on day 13p.i. and TGF-&#946; levels on days 6 and 13p.i. Lung macrophages from non-infected rats cultured with live NBL showed a significant increase in arginase activity and NO levels. Live and dead NBL induced a significant increase in arginase activity in lung macrophages from infected rats. Only live NBL significantly increased NO levels in these macrophages. The present work demonstrates for the first time, the emergence of regulatory parameters in the early lung immune response during T. spiralis infection. The immumodulatory properties exerted by NBL during its passage through this organ could be the cause of such regulation. Moreover, we have shown the ability of NBL to activate macrophages from the lung parenchyma by the classical and alternative pathways.","palabras_clave":"Trichinella spiralis|newborn larvae|lung|regulatory T cells|alternatively activated macrophages|TGF-&#946;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4400,"idioma_id":4,"titulo":". Mut S regulates access of the error- prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity","resumen":"Translesion DNA polymerases (Pol) function in the bypass of template lesions to relieve stalled replication forks but also display potentially deleterious mutagenic phenotypes that contribute to antibiotic resistance in bacteria and lead to human disease. Effective activity of these enzymes requires association with ring-shaped processivity factors, which dictate their access to sites of DNA synthesis. Here, we show for the first time that the mismatch repair protein MutS plays a role in regulating access of the conserved Y-family Pol IV to replication sites. Our biochemical data reveals that MutS inhibits the interaction of Pol IV with the &#946; clamp processivity factor by competing for binding to the ring. Moreover, the MutS-&#946; clamp association is critical for controlling Pol IV mutagenic replication under normal growth conditions. Thus, our findings reveal important insights into a non-canonical function of MutS in the regulation of a replication activity.","palabras_clave":"DNA polymerase|Antibiotic resistance|MutS|beta clamp"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4401,"idioma_id":4,"titulo":"Rhizosphere associated bacteria trigger accumulation of terpenes in leaves of Vitis vinifera L. cv. Malbec that protect cells against reactive oxygen species","resumen":"It has been proposed that plant growth promoting rhizobacteria (PGPR) stimulate plant growth and development by inducing the biosynthesis of secondary metabolites, like terpenes, which reduce stress incidence. Three bacteria previously isolated from grapevine roots and adjacent soil (Microbacteriumimperiale Rz19M10, Kocuria erythromyxa Rt5M10 and Terribacillus saccharophilus Rt17M10) were tested as PGPR. After 30 days since root inoculation of in vitro grown Vitis vinifera cv. Malbec plants, the monoterpenes &#945;-pinene, terpinolene and 4-carene, and the sesquiterpene nerolidol were detected only in bacterized-plant leaves. Also, the concentrations of the diterpenes &#945; and &#947;-tocopherol, and the sterols sitosterol and lupeol were significantly enhanced compared to controls. The leaf extracts of bacterized plants showed photoprotective properties since they decreased the oxygen consumption (that is photooxidation)of the amino acid tryptophan in a sensitized solution, thus indicating an increment of the antioxidant capacity of the tissues. In addition, experiments with &#945;-pinene and nerolidol standards showed the capability to intercept reactive oxygen species in the sensitized solution. Moreover, bacterized plants infected with the pathogen Botrytis cinerea showed a reduction in the lesion diameter compared with non-bacterized plants. The results suggest that M. imperial, K. erythromyxa and mainly T.saccharophilus are able to induce a systemic response that trigger increases on monoterpenes, sesquiterpenes, tocopherols and membrane sterols. These compounds enhance the antioxidant capacity in leaf tissues that may help grapevine to cope with stresses.","palabras_clave":"VITIS VINIFERA L|MICROBACTERIUM IMPERIALE|KOCURIA ERYTROMYXA|TERRIBACILLUS SACCHAROPHILUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4402,"idioma_id":4,"titulo":"CO-EXPRESSION OF WILD-TYPE P2X7R WITH GLN460ARG VARIANT ALTERS RECEPTOR FUNCTION","resumen":"The P2X7 receptor is a member of the P2X family of ligand-gated ion channels. A single-nucleotide polymorphism leading to a glutamine (Gln) by arginine (Arg) substitution at codon 460 of the purinergic P2X7 receptor (P2X7R) has been associated with mood disorders. No change in function (loss or gain) has been described for this SNP so far. Here we show that although the P2X7R-Gln460Arg variant per se is not compromised in its function, co-expression of wild-type P2X7R with P2X7R-Gln460Arg impairs receptor function with respect to calcium influx, channel currents and intracellular signaling in vitro. Moreover, co-immunoprecipitation and FRET studies show that the P2X7R-Gln460Arg variant physically interacts with P2X7R-WT. Specific silencing of either the normal or polymorphic variant rescues the heterozygous loss of function phenotype and restores normal function. The described loss of function due to co-expression, unique for mutations in the P2RX7 gene so far, explains the mechanism by which the P2X7R-Gln460Arg variant affects the normal function of the channel and may represent a mechanism of action for other mutations.","palabras_clave":"PX27R|Gln460Arg|Heterodimerization|receptor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4403,"idioma_id":4,"titulo":"SCHEDULE-DEPENDENT ANTIANGIOGENIC and CYTOTOXIC EFFECTS of CHEMOTHERAPY on VASCULAR ENDOTHELIAL and RETINOBLASTOMA CELLS","resumen":"Current treatment of retinoblastoma involves using the maximum dose of chemotherapy that induces tumor control and is tolerated by patients. The impact of dose and schedule on the cytotoxicity of chemotherapy has not been studied. Our aim was to gain insight into the cytotoxic and antiangiogenic effect of the treatment scheme of chemotherapy used in retinoblastoma by means of different in vitro models and to evaluate potential effects on multi-drug resistance proteins. Two commercial and two patient-derived retinoblastoma cell types and two human vascular endothelial cell types were exposed to increasing concentrations of melphalan or topotecan in a conventional (single exposure) or metronomic (7-day continuous exposure) treatment scheme. The concentration of chemotherapy causing a 50% decrease in cell proliferation (IC50) was determined by MTT and induction of apoptosis was evaluated by flow cytometry. Expression of ABCB1, ABCG2 and ABCC1 after conventional or metronomic treatments was assessed by RT-qPCR. We also evaluated the in vivo response to conventional (0.6 mg\/kg once a week for 2 weeks) and metronomic (5 days a week for 2 weeks) topotecan in a retinoblastoma xenograft model. Melphalan and topotecan were cytotoxic to both retinoblastoma and endothelial cells after conventional and metronomic treatments. A significant decrease in the IC50 (median, 13-fold; range: 3-23) was observed following metronomic chemotherapy treatment in retinoblastoma and endothelial cell types compared to conventional treatment (p0.05). In mice, continuous topotecan lead to significantly lower tumor volumes compared to conventional treatment after 14 days of treatment (p<0.05). Continuous exposure to melphalan or topotecan increased the chemosensitivity of retinoblastoma and endothelial cells to both chemotherapy agents with lower IC50 values compared to short-term treatment. These findings were validated in an in vivo model. None of the dosing modalities induced multidrug resistance mechanisms while apoptosis was the mechanism of cell death after both treatment schedules. Metronomic chemotherapy may be a valid option for retinoblastoma treatment allowing reductions of the daily dose.","palabras_clave":"RETINOBLASTOMA|CYTOTOXICITY|METRONOMICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4404,"idioma_id":4,"titulo":"An experimental model of affinity filtration for the isolation of egg white Lysozyme using Cibacron Blue immobilized to yeast cells","resumen":"An experimental model of affinity filtration process was designed using a macroligand composed by Cibacron Blue F3GA immobilized to yeast cells. Its performance was evaluated, at bench scale, throug hthe recovery of egg white Lysozyme. The selective and reversible binding between the Cibacron ligand molecule and the enzyme is described. The separation of Lysozyme from the protein mixture included the application of stages such as affinity adsorption, concentration, diafiltration and elution. A tangential microfiltration system with an inorganic membrane was designed. The main finding was the development of the diafiltration operation, key stage in the enzyme isolation. The macroligand particle kept its integrity along the whole process and the degree of purity of the isolated Lysozyme was significant.","palabras_clave":"AFFINITY FILTRATION|MACROLIGAND|CIBACRON BLUE|LYSOZYME ISOLATION|HEN EGG WHITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4405,"idioma_id":4,"titulo":"XacFhaB adhesin, an important Xanthomonas citri ssp. citri  virulence factor, is recognized as a pathogen-associated  molecular pattern","resumen":"Adhesion to host tissue is one of the key steps of the bacterial pathogenic process. Xanthomonas citri subsp. citri has a non-fimbrial adhesin protein XacFhaB required for bacterial attachment, which we have previously demonstrated to be an important virulence factor for the development of citrus canker. XacFhaB is a 4,753 residue long protein with a predicted &#61538;-helical fold structure, involved in bacterial aggregation, biofilm formation and adhesion to the host. In this work, to further characterize this protein and considering its large size, XacFhaB was dissected into three regions based on bioinformatic and structural analyses, for functional studies. First, the capacity of these protein regions to aggregate bacterial cells was analyzed. Two of these regions were able to form bacterial aggregates, being the most amino-terminal region dispensable for this activity. Moreover, XacFhaB has features resembling pathogen-associated molecular patterns (PAMPs), which are recognized by plants. As PAMPs activate plant basal immune responses, the role of the three XacFhaB regions as elicitors of these responses was investigated. All adhesin regions were able to induce basal immune responses in host and non-host plants, with a stronger activation by the carboxyl-terminal region. Furthermore, pre-infiltration of citrus leaves with XacFhaB regions impaired X. citri subsp. citri growth, confirming the induction of defense responses and restraint of citrus canker. This work reveals that adhesins from plant pathogens trigger plant defense responses, opening new roads for the development of protective strategies for disease control.","palabras_clave":"CITRUS CANKER|NON-FIMBRIAL ADHESIN|XANTHOMONAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4406,"idioma_id":4,"titulo":"Contrasting Phylogenetic and Diversity Patterns in Octodontoid Rodents and a New Definition of the Family Abrocomidae","resumen":"Octodontoidea is the most species-rich cladeamong hystricomorph rodents. Based on a combined parsimonyanalysis of morphological and molecular data of extinctand extant species, we analyze the history of SouthAmerican octodontoids and propose ages of divergence olderthan interpreted so far. Early Abrocomidae are recognized forthe first time, and a new definition of the family is provided.Traditionally accepted fossil-based times of origin for thesouthern clades are reinterpreted as later stages of differentiationmarkedly uncoupled from the origin, differentiation implyingspecializations for open environments as shown in amorphospace of skull variation. Origin of crown groups is alsostrongly uncoupled from origin of clades as a consequence ofextinction of deep lineages. In the resulting diversity pattern ofmodern southern clades of octodontoids, the combination ofgreater disparity, less content of evolutionary history, and lowertaxonomic diversity, compared to their northern counterparts,appears at first counterintuitive.We propose that primarycomponents of diversity derived from evolutionary transformationor anagenesis, on the one hand, and from cladogenesisand extinction, on the other, should not be consideredassociated, or at least not necessarily. Certain patterns of relationships between these distinct components could bedriven by environmental dynamics. Like environments,octodontoid diversity would have been more stable in northernSouth America, whereas in the south, both strong adaptivechange and extinction would have been triggered by emergingderived environments.","palabras_clave":"RODENTIA|SOUTH AMERICAN OCTODONTOIDEA|ABROCOMIDAE|PHYLOGENY|DIVERGENCE TIMES|DIVERSITY PATTERNS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4407,"idioma_id":4,"titulo":"'Development of a Methodology to Estimate Ghg´s Produced by the Beef Chain in Argentina","resumen":"The world is nowadays facing a great challenge: the production of food in harmony with the environment. Air pollution resulting from livestock activities and the existing possible solutions are shown as elements of vital importance. This fact definitely leads us to consider this issue from the global, national and local point of view. Each country faces different situations in terms of emissions which result directly from the production activities circumstances. Livestock in Argentina represents a substantial portion of the agricultural activities. Therefore, emissions produced become critical to any climate change mitigation and adaptation policy.Currently, satellite data can be obtained from an atmosphere scanning with territorial and temporal segregation. This information makes possible to achieve a global worldwide coverage, however, of low accuracy. Although it is not possible to identify sources and specific sumps, data on large areas can be achieved.Within this context, this project5 has been framed mainly focused on the creation of estimates of greenhouse gas emissions (GHG) produced by the beef value chain in Argentine on different production scenarios.This Study has been carried out and promoted by the Ministry of Agriculture, Livestock and Fisheries, managed and coordinated by the National University of Tres de Febrero -UNTREF- and developed under the technical advice of AACREA [Argentine Association of Regional Consortiums for Agricultural Experimentation]. The purpose of this paper is to discuss the development of a model in order to estimate emissions of greenhouse gases in the Argentine beef chain on different scenarios for the 2013-2014 period and to propose, on said basis, production models and functional marketing in line with the environmental objectives.","palabras_clave":"Greenhouse gas emissions|livestock|estimates model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4408,"idioma_id":4,"titulo":"Soil microbial functionality in response to the inclusion of cover crop mixtures in agricultural systems","resumen":"Agricultural systems where monoculture prevails are characterized by fertility losses and reduced contribution to ecosystem services. Including cover crops (CC) as part of an agricultural system is a promising choice in sustainable intensification of those demanding systems. We evaluated soil microbial functionality in cash crops in response to the inclusion of CC by analyzing soil microbial functions at two different periods of the agricultural year (cash crop harvest and CC desiccation) during 2013 and 2014. Three plant species were used as CC: oat (Avena sativa L.), vetch (Vicia sativa L.) and radish (Raphanus sativus L.) which were sown in two different mixtures of species: oat and radish mix (CC1) and oat, radish and vetch mix (CC2), with soybean monoculture and soybean\/corn being the cash crops. The study of community level physiological profiles showed statistical differences in respiration of specific C sources indicating an improvement of catabolic diversity in CC treatments. Soil enzyme activities were also increased with the inclusion of CC mixtures, with values of dehydrogenase activity and fluorescein diacetate hydrolysis up to 38.1% and 35.3% higher than those of the control treatment, respectively. This research evidenced that CC inclusion promotes soil biological quality through a contribution of soil organic carbon, improving the sustainability of agrosystems. The use of a CC mixture of three plant species including the legume vetch increased soil biological processes and catabolic diversity, with no adverse effects on cash crop grain yield. © 2016 INIA.","palabras_clave":"microorganisms|soil functionality|diversification|enzymes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4409,"idioma_id":4,"titulo":"Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3- beta-HSD and ARO but lower 5-alpha-R-1 in male rats","resumen":"dietary phytoestrogens causes obesity and diabetes in adult male rats. Based on the facts that hypothalamic food intake control is disrupted in phytoestrogen-deprived animals and that several steroids affect food intake, we hypothesized that phytoestrogen withdrawal alters the expression of hypothalamic steroidogenic enzymes. Male Wistar rats fed with a high-phytoestrogen diet from conception to adulthood were subjected to phytoestrogen withdrawal by feeding them a low-phytoestrogen diet, or a high phytoestrogen?high fat diet. Withdrawal of dietary phytoestrogens increased 3&#61538;&#61485;HSD and ARO gene expression and decreased those of 5&#61537;R-1. This is a direct effect of the lack of dietary phytoestrogens and not a consequence of obesity, as it was not observed in high-fat fed rats. Phytoestrogen withdrawal and high-fat diet intake reduced hypothalamic expression of ER&#61537;&#61472;correlated with low levels of ER&#61537;-O, ER&#61537;-OS and ER&#61537;-OT transcripts. Variations in gene expression of steroidogenic enzymes may affect the content of neurosteroids. As neurosteroids are related to food intake control, the changes observed may be a novel mechanism in the regulation of energy balance in obese phytoestrogen-deprived animals. In rats, steroidogenesis and estrogen receptor signaling appear to be altered by phytoestrogen withdrawal in the rat.  The ubiquity of phytoestrogens in the diet and changing intakes or withdrawal suggest that aspects of human health could be affected based on the rat and warrant further research.","palabras_clave":"phytoestrogens|obesity|hypothalamus|steroidogenic enzymes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4410,"idioma_id":4,"titulo":"First report of parasitism by Hexametra boddaertii (Nematoda: Ascaridae) in Oxyrhopus guibei (Serpentes: Colubridae)","resumen":"The current study summarizes the postmortem examination of a specimen of Oxyrhopus guibei (Serpentes,Colubridae) collected in Iguazu National Park (Argentina), and found deceased a week following arrival tothe serpentarium of the National Institute of Tropical Medicine (Argentina). Although the snake appearedto be in good health, a necropsy performed following its death identified the presence of a large numberof roundworms in the coelomic cavity, with indications of peritonitis and serosal adherence. Additionalobservations from the necropsy revealed small calcifications in the mesothelium of the coelomic cavity;solid and expressive content in the gallbladder; massive gastrointestinal obstruction due to nematodes;and lung edema and congestion. Histopathological analyses of lung sections also showed proliferativeheterophilic and histiocytic pneumonia. Parasites isolated from both the intestine and coelomic cavitywere identified as Hexametra boddaertii by a combination of light and scanning electron microscopicexamination. Results from this necropsy identify O. guibei as a new host for H. boddaertii, and is thefirst report of a natural infection by Hexametra in Argentina. Since Hexametra parasites may contributeto several pathological conditions in humans, and with the recent availability of O. guibei specimensthrough the illegal pet trade, it is necessary to consider the possibility of zoonotic helminth transmissionof Hexametra from snake to human","palabras_clave":"Oxyrhopus guibei|Colubridae|SERPENTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4411,"idioma_id":4,"titulo":"Extending the duration of treatment with progesterone and treatment with eCG improves fertility in suckled beef cows with low body condition score subjected to timed artificial insemination","resumen":"The objective of this study was to evaluate the effect of an extended progesterone treatment on follicular development and fertility in postpartum, suckled beef cows subjected to timed artificial insemination (TAI). In experiment 1, cows (n = 24) with body condition score (BCS) &#8805;4.5 received either a 2-g progesterone intravaginal device on Day -23 or a 0.558-g progesterone intravaginal device on Day -9. Then, all cows received 2 mg of estradiol benzoate on Day -9; removal of the device, 1-mg estradiol cypionate, and PGF2&#945; on Day -2; and TAI on Day 0. Metabolic status was assessed between Days -9 and -2. Ovarian structures and plasma progesterone were determined weekly from Day -23 to -9, daily from Day -9 to 0, and weekly until Day 28. In experiment 2, cows (n = 302) with BCS &#8805;4.5 received identical treatment to cows in experiment 1, but on Day -2, cows received 400 IU of two different commercial preparations of equine chorionic gonadotropin (eCG). Ovarian structures were determined on Days -23 and -9 on a subset of cows (n = 40). Pregnancy was determined 39 days after TAI. In experiment 3, multiparous cows (n = 244) with BCS <5.0 received identical treatment as cows in experiment 1 initiated on Day -18, and on Day -2, cows received 400 IU of eCG or no treatment. Ovarian structures were determined in a subset of cows (n = 31) on Days -3, -2, -1, 0, 1, and on Day 10. Pregnancy was determined 39 days after TAI. The results indicated that in experiment 1, plasma progesterone was higher in treated than nontreated (control cows) during the first 14 days (P = 0.0001). The extended progesterone treatment increased the size of the largest follicle between Days -23 and Day -5 (Group by Day, P = 0.04) and tended to increase the size of the dominant follicle from Day -5 to Day -1 (Group by Day, P = 0.06). There was no effect of metabolic status or interaction between metabolic status and day on follicular growth. In experiment 2, extended progesterone treatment tended to increase the size of the largest follicle between Day -23 and -9 (P = 0.06). There was no effect of Group, eCG, BCS, and parity on pregnancy per AI. In experiment 3, extended progesterone treatment combined with eCG increased the size of the dominant follicle (P = 0.01). Both extended progesterone treatment (P = 0.02) and eCG (P = 0.03) increased pregnancy per AI. In conclusion, an extended progesterone treatment stimulated follicular growth postpartum and improved fertility only in cows with low BCS.","palabras_clave":"FERTILITY|ECG|PROGESTERONE|BEEF"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4412,"idioma_id":4,"titulo":"Grape pomace and grape pomace extract improve insulin signaling in high-fat-fructose fed rat-induced metabolic syndrome","resumen":"In this study the effect of diet supplementation with grape pomace (GP) and grape pomace extract (GPE) on insulin sensitive tissues (adipose, liver and muscle) was evaluated in an experimental model of metabolic syndrome (MetS). MetS was developed by giving a high-fat-fructose (HFF) diet to Wistar rats. Six weeks of HFF diet induced weight gain, which was partially attenuated by GP (1 g per kg per day) and GPE (300 mg per kg per day) supplementation. HFF diet increased systolic blood pressure, triglycerides, insulin resistance (HOMA:IR) and inflammation (c-reactive protein (CRP)). Supplementation with GP prevented SBP, triglycerides and CRP increased and partially attenuated insulin resistance. On the other hand, GPE partially reduced SBP and triglycerides and significantly prevented insulin resistance and inflammation. Also, HFF diet induced higher triglycerides content and enhanced NADPH oxidase activity in the liver. Also, HFF diet increased the epididymal adipose tissue weight, enlarged adipocyte size, and c-jun N-terminal kinase (JNK) activation, probably contributing to a pro-inflammatory cytokine pattern (higher resistin) and lower adiponectin protein expression. These alterations may result in an impairment of insulin signaling cascade observed in adipose, liver and muscle tissue (IRS1, Akt, and extracellular signal-regulated kinases (ERK1\/2)) from HFF rats. Supplementation with GP and to a greater extent GPE attenuated liver triglyceride content and adiposity and restored adipose, liver and muscle response to insulin. These findings show that supplementation with GP and GPE to a greater extent can counteract adiposity, inflammation, liver damage and impaired insulin signaling associated to MetS, supporting the utilization of winemaking residues in food industry\/human health due to their high amount of bioactive compounds.","palabras_clave":"pomace extract|insulin sensitive tissues|metabolic syndrome|winemaking residues"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4413,"idioma_id":5,"titulo":"Análisis de redes sociales: un caso práctico","resumen":"Las redes sociales son consideradas como nuevos modos de socialización, a partir de ellas se puede tener una fuente de interacción entre las personas posibilitando la contextualización de fenómenos sociales entre los indivíduos y las relaciones inherentes que han surgido. Las diferentes herramientas computacionales junto con las métricas que brindan, sirven como base de conocimiento desde su aplicabilidad práctica sobre un tópico relevante como lo es el Análisis de Redes Sociales (ARS). Haciendo foco en Gephi como una herramienta de ARS, simple de usar y comprender en cuanto a métricas y visualizaciones, analizaremos un caso de estudio práctico en el ámbito educativo donde evaluaremos la dinámica de comunicación en un foro asíncrono dentro del contexto del ARS. Se evidenciará que las métricas del ARS y la visualización de la estructuración de los nodos y de las interacciones son una vía útil y potencialmente efectiva para analizar patrones de interacción en línea. Integrar esta aproximación del ARS al contexto educativo, es un medio que le permite al profesor detectar y diagnosticar el clima social e intervenir de acuerdo a los resultados obtenidos. Finalmente, determinaremos conclusiones acerca de esta metodología y su utilidad en este dominio, como así también las líneas de trabajo futuro.","palabras_clave":"ANÁLISIS DE REDES SOCIALES|HERRAMIENTAS SOFTWARE|MÉTRICAS|REDES SOCIALES|GEPHI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4414,"idioma_id":4,"titulo":"Patterns of resource distribution among conspecific larvae in two fruit fly species: Anastrepha fraterculus and Ceratitis capitata (Diptera: Tephritidae)","resumen":"1 Anastrepha fraterculus (Diptera: Tephritidae) and Ceratitis capitata (Diptera: Tephritidae) are fruit fly pests whose larvae are frequently found sharing a fruit with conspecifics. As larvae are incapable of leaving the fruit in search of non-infested fruit, they are forced to share nutritional resources, and eventually compete for them with other larvae. 2 Here we studied the effect of intraspecific competition on the development of A. fraterculus and C. capitata larvae, and compared the strategies adopted by these species. 3 To this end, newly hatched larvae were transferred into a container with a fixed amount of larval diet at increasing larval densities and several developmental parameters were measured. 4 The two species showed similarities and differences in the way in which they responded to an increasing density. In A. fraterculus pupal weight and the duration of the larval stage decreased as the competition levels increased. Larvae of C. capitata showed a consistent reduction in pupal weight and in larval survival as larval density increased. In A. fraterculus the reduction in pupal weight was heterogeneous, with most pupae showing a marked decrease in weight and only few pupae showing slight negative effects or no effect at all, whereas in C. capitata, the reduction was similar for all the pupae.5 The differences in the way in which these species responded to competition might evidence a different pattern of resource distribution among conspecific larvae. The implication of these findings on oviposition behavior and life history of the two species is discussed.","palabras_clave":"Anastrepha|Ceratitis|Competition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4416,"idioma_id":4,"titulo":"CHARACTERIZATION OF PANETH CELLS IN ALPACAS  (Vicugna pacos, MAMMALIA, CAMELIDAE)","resumen":"Paneth cells are secretory epithelial cells of the innate immune system of the intestine of several mammals, including alpacas. Little is known about them in this species; thus, in the present study we described the morphology and histochemical characteristics of Paneth cells in healthy fetuses, and young and adult alpacas. For this purpose, samples of duodenum, jejunum and ileum were taken from 6 fetuses at different days of pregnancy (between days 221-330), 66offsprings (between 0 and 45-days-old)  and 5 adult alpacas (2-years-old). Samples were fixed in 10% buffered formalin and processed for histological and morphometrical analysis using HE and Masson Trichomic´s technique. Immunohistochemistry was used to identify Paneth cells using anti-lysozyme antibody. In addition, the lectinhistochemichal binding-pattern of Panethcell´s granules was evaluated. Lyzozyme was immunohistochemically detected in the granules ofPaneth cells from day 283 of pregnancy in all the small intestinal sections of the studied fetuses. In newborn alpacas Paneth cells were initially found in the duodenum, but the following days (days 18-21 after birth) they were also found in the ileum. Their size gradually increased after birth, but then no significant differences were found. In adult alpacas the number waslower than offsprings. We suggest that Paneth cells early differentiate in the small intestine of alpacas, and the increase in their number during the first two weeks of life strongly support their possible involvement in the intestinal defensive functions against the enteric diseases that occur during the lactancy stage.","palabras_clave":"Paneth Cells|Alpacas|Morphometry|Immunohistochemistry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4418,"idioma_id":4,"titulo":"Stat3 regulates ErbB-2 expression and co-opts ErbB-2 nuclear function to induce miR-21 expression, PDCD4 downregulation and breast cancer metastasis","resumen":"Membrane overexpression of the receptor tyrosine kinase ErbB-2 (MErbB-2) accounts for a clinically aggressive breast cancer (BC) subtype (ErbB-2-positive) with increased incidence of metastases. We and others demonstrated that nuclear ErbB-2 (NErbB-2) also plays a key role in BC and is a poor prognostic factor in ErbB-2-positive tumors. The signal transducer and activator of transcription 3 (Stat3), another player in BC, has been recognized as a downstream mediator of MErbB-2 action in BC metastasis. Here, we revealed an unanticipated novel direction of the ErbB-2 and Stat3 interaction underlying BC metastasis. We found that Stat3 binds to its response elements (GAS) at the ErbB-2 promoter to upregulate ErbB-2 transcription in metastatic, ErbB-2-positive BC. We validated these results in several BC subtypes displaying metastatic and non-metastatic ability, highlighting Stat3 general role as upstream regulator of ErbB-2 expression in BC. Moreover, we showed that Stat3 co-opts NErbB-2 function by recruiting ErbB-2 as its coactivator at the GAS sites in the promoter of microRNA-21 (miR-21), a metastasis-promoting microRNA (miRNA). Using an ErbB-2 nuclear localization domain mutant and a constitutively activated ErbB-2 variant, we found that NErbB-2 role as a Stat3 coactivator and also its direct role as transcription factor upregulate miR-21 in BC. This reveals a novel function of NErbB-2 as a regulator of miRNAs expression. Increased levels of miR-21, in turn, downregulate the expression of the metastasis-suppressor protein programmed cell death 4 (PDCD4), a validated miR-21 target. Using an in vivo model of metastatic ErbB-2-postive BC, in which we silenced Stat3 and reconstituted ErbB-2 or miR-21 expression, we showed that both are downstream mediators of Stat3-driven metastasis. Supporting the clinical relevance of our results, we found an inverse correlation between ErbB-2\/Stat3 nuclear co-expression and PDCD4 expression in ErbB-2-positive primary invasive BCs. Our findings identify Stat3 and NErbB-2 as novel therapeutic targets to inhibit ErbB-2-positive BC metastasis.","palabras_clave":"BREAST CANCER METASTASIS|NUCLEAR ERBB-2|STAT3|MIR-21"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4419,"idioma_id":4,"titulo":"PKCd inhibition impairs mammary cancer proliferative capacity but selects cancer stem cells, involving autophagy","resumen":"Triple negative breast cancers are poorly differentiated tumors. The resistance to cytotoxicagents and the relapse displayed by this class of mammary malignancies has been attributedto the presence of cancer stem cells (CSC). Protein kinase C (PKC) family regulates diversecellular functions including cell death, proliferation and survival. Recent studies havereported that PKC isoenzymes, in particular PKC&#948;, are involved in apoptosis induction orautophagy. In the present study we focused on how PKC&#948; regulates proliferation andcancer stem cell properties of the hormone- independent mammary cancer cell line LM38-LP, using pharmacological and genetic approaches. We found that pharmacologicalinhibition of PKC&#948; impairs in vitro LM38-LP proliferation through cell cycle arrest andformation of cytoplasmic-vacuoles. By inmunoflorescence we confirmed that Rottlerintreatment induced the apparition of LC3 dots in the cytoplasm, as PKC&#948; inhibitionincreased autophagy flux. PKC&#948; inhibition increased CSC growth rate and self-renewalwhich was associated with the induction of Nanog, Sox2, Slug and Sox9. CSC pre-tretaedwith Rottlerin developed a ?grape-like? morphology, usually associated to a malignantphenotype. The knockdown of PKC&#948; induced autophagy and increased CSC number, sothese effects seem to be exerted through a PKC&#948; dependent pathway. Finally, we couldobserve that an increment in the number of mammospheres was reverted under 3MAtreatment, suggesting that autophagy is necessary for the increased of CSC self-renewal byPKC&#948;. We demonstrated that PKC&#948; exerts a dual role through the autophagy mechanism,decreasing proliferative capacity of mammary tumor cells and regulating tumor stem cellself-renewal.","palabras_clave":"PROTEIN KINASE C DELTA|MAMMARY CANCER|CANCER STEM CELLS|AUTOPHAGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4420,"idioma_id":4,"titulo":"In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus","resumen":"Lectins are glycan-binding proteins that are resistant to digestion in the gastrointestinal tract and enter intact to blood circulation. The aim of this study was to evaluate the influence of edible mushroom Agaricus bisporus lectin (ABL) on innate and adaptive immune responses as well as its effect in two different experimental pathologies that involve the immune system. ABL inhibited in vitro nitric oxide (NO) production by mouse peritoneal macrophages in response to the proinflammatory stimuli lipopolysaccharides (LPS) but did not modify the activity of arginase, showing that while ABL downregulates M1 activation it does not affect M2 activation. ABL also inhibited mononuclear cell proliferation in response to mitogen Con A or in a mixed lymphocyte reaction. During the in vivo studies, oral administration of ABL to BALB\/c mice induced a marked inhibition of NO production by peritoneal macrophages after LPS stimuli. The influence of ABL on tumor growth was studied in BALB\/c mice receiving daily oral doses of ABL and implanted with CT26 tumor cells. ABL treatment induced significantly higher rate of tumor growth when compared with control mice. On the other hand, oral ABL administration in Wistar rats induced a marked diminution of the disease incidence and severity of the clinical signs of experimental autoimmune encephalomyelitis. We can conclude that ABL has an in vivo immunomodulatory effect reducing the innate and adaptive responses. This food lectin shows potential therapeutic application on control of inflammatory autoimmune pathologies.","palabras_clave":"food lectin|autoimmune encephalomyelitis|lymphocytes|immunomodulation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4422,"idioma_id":5,"titulo":"Nuevas determinaciones de velocidades de ondas P y ondas S para la corteza  sísmica del terreno Cuyania en el retroarco andino","resumen":"In this study a crustal model (1D) of P and S wave seismic velocities is independently obtained for the region located between 30°?32.5° S and 67°?68.5° W of the Andean, at about 450 km east from the trench.To determine this crustal model 514 local earthquakes data were used. These earthquakes had local magnitudes between 0.8 &lt; ML&lt; 4.8 and had occurred between July 1996 and January 2014, and were reported by the permanent seismic network catalog of the Argentinean National Institute on Seismic Prevention (INPRES). For these earthquakes, the P and S wave arrival times were manually read in 37 seismic stations. Thus, it was possible to obtain the seismic location of each earthquake using weighing for quality and distance, and a seismic velocity model previously obtained by other studies.","palabras_clave":"basamento|velocidad sísmica|inversión"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4423,"idioma_id":4,"titulo":"Fire as mediator of pine invasion: evidence from Patagonia, Argentina","resumen":"Fire has been found to promote or halt biological invasions. Pine trees (genus Pinus) are highly invasive in the southern hemisphere and the effect of fire on their invasion ability is not clear. An analysis of Pinus spreading after wildfires in Patagonia reveals that there is a high risk of pines becoming invasive if ignition frequency increases in Patagonia. Also plantations could increase fire intensity and\/or frequency in Patagonia, creating a potential positive feedback between invasion and wildfires. The effect of fires on pine invasion was modulated by precipitation. In areas where precipitation was lower than 800 mm fires did not promote pine invasion. However, in areas with higher precipitation there was a strong promotion of pine invasion by fire, after a lag time. These results show that fire, a disturbance more frequent now in the region, can promote pine invasion.","palabras_clave":"PINE PLANTATIONS|SPREADING|TIME LAG|WILDFIRES|PRECIPITATION GRADIENT|TREE INVASIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4425,"idioma_id":5,"titulo":"Immunotoxicity of sublethal synthetic acaricide doses and\/or parasitic infection in nurse honey bees","resumen":"Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apiculturalmatrices. In this work, the effects of parasitosis\/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and\/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate the following: (1) honeybee survival was not affected by any of the treatments; (2) parasite development was not altered by acaricide treatments; (3) coumaphos exposure decreased lysozyme expression; (4) N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.","palabras_clave":"HONEY-BEE|ACARICIDES|INMUNOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4427,"idioma_id":4,"titulo":"All hormone-producing cell types of the pituitary intermediate and anterior lobes derive from Prop1 expressing progenitors","resumen":"Mutations in PROP1, the most common known cause of combined pituitary hormone deficiency in humans, can result in the progressive loss of all hormones of the pituitary anterior lobe. In mice, Prop1 mutations result in the failure to initiate transcription of Pou1f1 (also known as Pit1) and lack somatotropins, lactotropins, and thyrotropins. The basis for this species difference is unknown. We hypothesized that Prop1 is expressed in a progenitor cell that can develop into all anterior lobe cell types, and not just the somatotropes, thyrotropes, and lactotropes, which are collectively known as the PIT1 lineage. To test this idea, we produced a transgenic Prop1-cre mouse line and conducted lineage-tracing experiments of Prop1-expressing cells. The results reveal that all hormone-secreting cell types of both the anterior and intermediate lobes are descended from Prop1-expressing progenitors. The Prop1-cre mice also provide a valuable genetic reagent with a unique spatial and temporal expression for generating tissue-specific gene rearrangements early in pituitary gland development. We also determined that the minimal essential sequences for reliable Prop1 expression lie within 10 kilobases of the mouse gene and demonstrated that human PROP1 can substitute functionally for mouse Prop1. These studies enhance our understanding of the pathophysiology of disease in patients with PROP1 mutations.","palabras_clave":"PROP1|PITUITARY|STEM CELLS|CRE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4428,"idioma_id":4,"titulo":"Noise generator for tinnitus treatment based on look-up tables","resumen":"Abstract. Treatment of tinnitus by means of masking sounds allows to obtain a significant improve of the quality of life of the individual that suffer that condition. In view of that, it is possible to develop noise synthesizers based on random number generators in digital signal processors (DSP), which are used in almost any digital hearing aid devices. DSP architecture have limitations to implement a pseudo random number generator, due to it, the noise statistics can be not as good as expectations. In this paper, a technique to generate additive white gaussian noise (AWGN) or other types of filtered noise using coefficients stored in program memory of the DSP is proposed. Also, an implementation of the technique is carried out on a dsPIC from Microchip. Objective experiments and experimental measurements are performed to analyze the proposed technique. ","palabras_clave":"NOISE GENERATOR|TINNITUS TREATMENT|DSP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4429,"idioma_id":4,"titulo":"Surface Morphological Modification of Cross linked Hydrophilic Co-Polymers by Nanosecond Pulsed Laser Irradiation","resumen":"This work reports an investigation of the surface modifications induced by irradiation with nanosecond laser pulses of ultraviolet and visible wavelengths on crosslinked hydrophilic co-polymeric materials, which have been functionalized with 1-vinylimidazole as a co-monomer. A comparison is made between hydrogels differing in the base co-monomer (N,N-dimethylaminoethyl methacrylate and N-[3-(dimethylamino)propyl] methacrylamide) and in hydration state (both swollen and dried states). Formation of craters is the dominant morphological change observed by ablation in the visible at 532 nm, whereas additional, less aggressive surface modifications, chiefly microfoams and roughness, are developed in the ultraviolet at 266 nm. At both irradiation wavelengths, threshold values of the incident laser fluence for the observation of the various surface modifications are determined under single-pulse laser irradiation conditions. It is shown that multiple-pulse irradiation at 266 nm with a limited number of laser shots can be used alternatively for generating a regular microfoam layer at the surface of dried hydrogels based on N,N-dimethylaminoethyl methacrylate. The observations are rationalized on the basis of currently accepted mechanisms for laser-induced polymer surface modification, with a significant contribution of the laser foaming mechanism. Prospective applications of the laser-foamed hydrogel matrices in biomolecule immobilization are suggested.","palabras_clave":"polymer|laser ablation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4430,"idioma_id":5,"titulo":"CARACTERIZACIÓN DEL ESTRÉS DE NITROGENO EN PAPA PARA INDUSTRIA USANDO EL NDVI","resumen":"La medición de la reflectancia con sensores ópticos tiene el potencial de proporcionar información sobre el estatus de nitrógeno (N) durante el ciclo del cultivo de papa para industria y podría predecir la dosis de N. Los objetivos de este trabajo fueron evaluar el estatus de N utilizando los sensores ópticos, GreenSeeker 506 (GS) y CropCircle ACS 210 (CC), compararlos y proporcionar algoritmos que pueden utilizarse para definir umbrales de suficiencia de N. Se realizaron tres experiencias en el cultivo de papa con dosis de N durante las campañas 2009\/10, 2010\/11 y 2012\/13. El rendimiento de los tubérculos aumentó por efecto de la fertilización nitrogenada y esto varió con la campaña agrícola. La relación entre los valores del índice de vegetación diferencial normalizada (NDVI) de los dos sensores fue significativa a lo largo del ciclo con elevados coeficientes de determinación (R2=0,74-0,99). Las lecturas del NDVI del CC fueron menores a las del GS a partir de los 60 ddp. La relación entre el NDVI relativo (RNDVI) con el N disponible (N a la plantación + dosis de N) se asoció significativamente a partir de los 60 días después de plantación (ddp) para el CC (R2=0,85, 0,91 y 0,65 para los 60, 80 y 100 ddp; respectivamente) y de los 80 ddp para el GS (R2=0,72 y 0,76 para los 80 y 100 ddp; respectivamente). El umbral de suficiencia de N promedio del RNDVI fue de 99,4% y 99,7% para el GS y CC; respectivamente. En general, los modelos de predicción del estrés de N variaron con el sensor y se determinó que el CC fue más sensible que el GS para determinar el estrés de N en etapas intermedias del ciclo del cultivo. ","palabras_clave":"GREENSEEKER 506|CROPCIRCLE ACS 210"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4431,"idioma_id":4,"titulo":"Habitat suitability models for the sand lizard Liolaemus wiegmannii based on landscape characteristics in temperate coastal dunes in Argentina","resumen":"The knowledge of environmental variables associated with the species occurrence allows the recognition of sites which fulfil ecological requirements eventually used for conservation of species. The coastal dunes of Argentina are inhabited by sand lizards. Anthropic activities have severely degraded this ecosystem, affecting the habitat structure at a large scale. In this context, the effects of landscape characteristics on the sand lizard´s (Liolaemus wiegmannii, Liolaemidae) presence were analysed to build habitat suitability maps along the coastal dunes of Argentina. A thematic map of study area was obtained from supervised classification of satellite images to identify landscape characteristics. Surveys were conducted during the lizard activity season, and landscape variables were measured in two spatial units. All information collected was compiled into a Geographic Information System. The relationship between the presence of lizards and landscape variables was evaluated by Generalized Linear Models. The predictions of these models were transferred by using Geographic Information System to habitat suitability maps. Almost all individuals (80%) were observed in semi-fixed dunes. The analysis of landscape metrics in the two spatial extents showed complementary results. The habitat suitability models suggest that: (i) heterogeneous landscapes composed by disaggregated patches of semi-fixed dunes and low or null percentages of active dunes distant from the coastline are the preferred environments, and (ii) human modifications such as urbanizations and forestation of dunes, have a negative impact on species occurrence. Suitable habitats were almost absent in those sectors of coastal dunes with highest level of urbanization, whereas they were distributed almost continuously in those areas without human disturbances.","palabras_clave":"landscape metrics|suitable habitats|sand lizard|GIS|Pampas coastal dunes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4432,"idioma_id":4,"titulo":"The Modulation of Liver Methylome in Liver Diseases","resumen":"Page and co-workers describes the role of DNA hydroxymethylation and Ten-Eleven-Translocation (TET) proteins in liver fibrogenesis. The authors robustly proved their hypothesis by using different approaches, including samples from freshly explanted primary sclerosing cholangitis livers and rat-hepatic stellate cells (rHSC) from experimental models of liver fibrosis, including chemically or bile duct ligation-induced fibrosis, and methionine-choline?deficient (MCD) diet. Based on the whole evidence, the authors concluded that DNA methylation \/ hydroxymethylation is a crucial step in HSC activation and therefore fibrogenesis. The importance of 5-Hydroxymethylcytosine (5-hmC) in the biology of non-cancerous human liver diseases, particularly nonalcoholic fatty liver disease (NAFLD), was recently described for the first time by our group (2). We observed that nonnuclear-5-hmC, which is probably located in  mitochondria, is lower in NAFLD as compared with controls,. Therefore, 5-hmC may be involved in the modulation of the mitochondrial DNA- methylome and gene expression, in particular components of the oxidative phosphorylation-chain, a finding that could explain much of the changes that occur during NAFLD-disease progression, including fibrogenesis. Of note, Page et el. observed that liver fibrosis in a rodent model of NAFLD (MCD) is accompanied by loss of 5-hmC and modulation of DNA (cytosine-5-)-methyltransferases (DNMTs) and TETs; specifically, the authors reported an induction of DNMT3a and DNMT3b during HSC transdifferentiation. In line with these findings, earlier data from our patient-oriented research in NAFLD demonstrated that hepatic expression of DNMT1 is significantly higher in the liver of NASH patients as compared with patients with simple steatosis; in fact, we examined the liver expression levels of the DNMT1 isoform in biopsy-proven NAFLD patients, and we observed that the disease severity is associated with a higher than 1000-fold increase in the DNMT1 transcriptional activity. Finally, Page et. al. observed a decrease in the expression of TET-proteins in both human and rat models tested, suggesting a major role for epigenetic mechanisms in HSC biology. Accordingly, we described the role of genetic variation in TET1 locus in the modulation of programmed liver-cell death during liver fat transformation, a finding that reinforces the concept of a potentially ?TET-mediated fine-tuning? of some of the molecular changes associated with liver injury that precede liver fibrosis. We found that subjects carrying the missense p.Ile1123Met variant (TET1-rs3998860) had significantly lower liver expression levels of TET1 protein in association with increased levels of circulating CK18 (caspase-mediated cleavage of the CK-18) levels (2). In addition, the TET2 locus may be involved in the modulation of the liver PPARGC1A methylation\/demethylation balance, which is altered in NAFLD (4); PPARGC1A is a main transcription factor modulating mitochondrial biogenesis and a sensor of changes in the cellular metabolism.Finally, by targeted deep sequencing of the entire TET2 locus, we found a low frequency missense variant, the rs62621450 (H1778R) (which is rare in Caucasian population, MAF 0.02) in three out of 360 subjects, who developed progressive NAFLD at early age (unpublished data) and also have family history of advanced liver disease. In conclusion, by examining the impact of epigenetic changes, including DNA hydroxymethylation and TET proteins, in the biology of NAFLD, a highly prevalent human liver disease that progress towards liver cirrhosis in a significant proportion of affected patients-, we agree with Page and co-workers that DNMTs and TETs may be potential new targets for treatment of NAFLD disease progression and fibrosis.","palabras_clave":"METHYLOME|TET FAMILY PROTEIN|DNA HYDROXYMETHYLATION|NAFLD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4433,"idioma_id":4,"titulo":"Heat Shock Protein 27 is down-regulated in Ballooned Hepatocytes of Patients with Nonalcoholic Steatohepatitis (NASH)","resumen":"Ballooning degeneration (BD) of hepatocytes is a distinguishing histological feature associated with the progression of nonalcoholic fatty liver disease (NAFLD). Under the assumption that NAFLD severity is associated with metabolic-stress we explored the hypothesis that heat shock 27&#8201;kDa protein 1 (HSP27), a protein chaperone involved in stress resistance and cytoskeletal-remodeling, might be deregulated in ballooned hepatocytes. We observed that fasting plasma glucose (fpG) (p&#8201;=&#8201;0.00002), total cholesterol (p&#8201;=&#8201;0.02) and triglycerides (p&#8201;=&#8201;0.01) levels, and female sex (p&#8201;=&#8201;0.01) were significantly associated with the presence of BD. A logistic regression model showed that BD was independently associated with fpG (p&#8201;=&#8201;0.002); OR per unit of glucose concentration 1.05, 95% confidence interval 1.02-1.09. Furthermore, BD was associated with a significant 2.24-fold decrease in the expression level of HSP27-mRNA in comparison with absence of ballooning, p&#8201;=&#8201;0.002. Ballooned hepatocytes showed very low HSP27 immunoreactivity compared with hepatocyes without ballooning (p&#8201;=&#8201;0.009); HSP27 immunoreactivity was inversely correlated with fpG levels (R: -0.49, p&#8201;=&#8201;0.01). In conclusion, BD is associated with down-regulation of liver HSP27 gene and protein expression, suggesting that ballooned hepatocytes fail to ensure a robust physiological response to metabolic-induced stress.","palabras_clave":"Heat Shock Protein 27|Ballooned Hepatocytes|NAFLD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4434,"idioma_id":4,"titulo":"Metabolite Signatures of Metabolic Risk Factors and their Longitudinal Changes.","resumen":"Context: Metabolic dysregulation underlies key metabolic risk factors?obesity, dyslipidemia, and dysglycemia.Objective: To uncover mechanistic links between metabolomic dysregulation and metabolic risk by testing metabolite associations with risk factors cross-sectionally and with risk factor changes over time.Design: Cross-sectional?discovery samples (N650; age36?69 years) from the Framingham Heart Study (FHS) and replication samples (N670; age61?76 years) from the BioImage Study, both following a factorial design sampled from high versus low strata of body mass index, lipids, and glucose. Longitudinal?FHS articipants (N554) with 5?7 years of follow-up for risk factor changes.Setting: Observational studies.Participants: Cross-sectional samples with or without obesity, dysglycemia, and dyslipidemia, excluding prevalent cardiovascular disease and diabetes or dyslipidemia treatment. Age and sex-matched by group.Interventions: None.Main Outcome Measure(s): Gas chromatography-mass spectrometry detected 119 plasma metabolites. Cross-sectional associations with obesity, dyslipidemia, and dysglycemia were tested in discovery, with external replication of 37 Metabolites. Single- and multi-metabolite markers were tested for association with longitudinal changes in risk factors.Results: Cross-sectional metabolite associations were identified with obesity (n26), dyslipidemia(n21), and dysglycemia (n11) in discovery. Glutamic acid, lactic acid, and sitosterol associated with all three risk factors in meta-analysis (p4.5x10?4). Metabolites associated with longitudinal risk factor changes were enriched for bioactive lipids. Multi-metabolite panels explained 2.5?15.3% of longitudinal changes in metabolic traits.Conclusions: Cross-sectional results implicated dysregulated glutamate cycling and amino acid metabolism in metabolic risk. Certain bioactive lipids were associated with risk factors cross-sectionally and over time, suggesting their upstream role in risk factor progression. Functional studies are needed to validate findings and facilitate translation into treatments or preventive measures.","palabras_clave":"METABOLOMICS|METABOLIC SYNDROME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4435,"idioma_id":4,"titulo":"How safe is Moderate Alcohol Consumption in Overweight and Obese Individuals?","resumen":"The future research agenda is open: More questions than answers The effect of MAC on NAFLD, and its association with obesity or overweightness are not only complex and multifaceted, but also difficult to measure. To strengthen the evidence and translate the results into clinical-decision making, we need answer various questions that would probably delineate a future patient-oriented research agenda. For example, the definition of MAC is arbitrary; so, we need to know exactly how much is safe, and how much is not in patients with NASH and fibrosis; further necessary definitions include the potential effect of drinking patterns and alcohol types, and whether the window between maximum protection and harm is the same in men and women. On the other hand, the potential ?cumulative-effect? of MAC on the modulation of factors involved in the pathogenesis of NAFLD should be carefully addressed; these include the inflammasome, gut microbiota and intestinal permeability, and obesity-related carcinogenesis. An additional challenge concerns the putative synergic effect of MAC and ?endogenous ethanol-synthesis in the gastrointestinal tract?, which could ameliorate the ?benefits? in specific clinical scenarios, such as obese patients with NASH and fibrosis {Engstler, 2015 ENGSTLER2015 \/id}.In summary, the future research agenda still remains open, looking for answers from cohort prospective studies elucidating the exact role of MAC on the disease progression of obese patients with NAFLD. In addition, further mechanistic studies are required to bring robust biological evidence on the long-term effects of MAC on the natural history of NAFLD and MetS.","palabras_clave":"ALCOHOL|OBESITY|NAFLD|MENDELIAN RANDOMIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4436,"idioma_id":4,"titulo":"Pretreatment Combination Reduces Remote Organ Damage Secondary to Intestinal Reperfusion Injury in Mice: Follow-up Study","resumen":"Background. Intestinal ischemia-reperfusion injury occurs after different surgicaltreatments, including intestinal transplantation. This harmful process may have an effectin remote organs, leading to multiple organ dysfunction syndrome and death. Therefore,to establish strategies to attenuate local and remote damage constitutes a challenge forexperimental and clinical surgeons in the intestinal surgical field.Methods. We evaluated the effect of ischemic preconditioning and tacrolimus pretreatmentapplied alone and in combination against local and remote damage caused by prolongedintestinal ischemia-reperfusion injury in a mouse model of warm ischemia.Results. Ischemic preconditioning applied alone and in combination with tacrolimusdecreased histological damage (P &lt; .05), number of apoptotic cells (P &lt; .05), nitrosativestress (P &lt; .01), and serum lactate dehydrogenase activity (P &lt; .05) and lowered uremia(P &lt; .05) compared with untreated post-reperfused intestines. Regarding remote organdamage, combination therapy was the unique condition able to attenuate lung (mainlyneutrophil infiltration and hemorrhage), liver (sinusoidal congestion and hepaticvacuolization), and kidney (acute tubular necrosis and hydropic degeneration)histological alterations (P &lt; .05), compared with the untreated group.Conclusions. These results support the application of these strategies in combination tominimize the impact of ischemia-reperfusion injury in the whole organism as a strategy toprevent multiple organ dysfunction syndromes and minimize the clinical impact.","palabras_clave":"reperfusion injury|remote organ damage|intestinal transplantation|tacrolimus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4437,"idioma_id":4,"titulo":"Growth and consumption of L-malic acid in wine-like medium by acclimated and non-acclimated cultures of Patagonian Oenococcus oeni strains","resumen":"Five Oenococcus oeni strains selected from spontaneous malolactic fermentation  (MLF) of Patagonic Pinot noir wine were assessed for their use as MLF starter  cultures. After the individual evaluation of tolerance to some stress conditions usually found in wine (pH, ethanol, SO2, and lysozyme), the behavior of the strains was analyzed in MLO broth with 14 % ethanol and pH 3.5 in order to test for the synergistic effect of high ethanol level and low pH and, finally, in a wine-like medium. Although the five strains were able to grow in MLO broth under low pH and\/or high ethanol, they must be acclimated to grow in a wine-like medium. Additionally, glycosidase and tannase activities were evaluated, showing differences among the strains. The potential of the strains to ferment citrate was tested and two of the five strains showed the ability to metabolize this substrate. We could not detect the presence of genes encoding histidine and tyrosine descarboxylase and putrescine carbamoyltransferase.  All the strains tested exhibited good growth capacity and ability to consume L-malic acid in a wine-like medium after cell acclimation, and each of them showed a particular enzyme profile that might confer the wine different organoleptic properties.","palabras_clave":"Oenococcus oeni|Patagonian wine|malic acid|wine-like medium"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4438,"idioma_id":5,"titulo":"Fotografía y sentido. Una aproximación pragmático-fenomenológica","resumen":"El presente escrito tiene como fin desplegar ciertas posiciones epistemológicas con el objetivo de indagar cómo nos relacionamos con las imágenes. En ese sentido, presentaremos algunas ideas para pensar una perspectiva que hemos denominado pragmática-fenomenológica, esa línea no sólo nos permitirá pensar algunas respuestas a nuestro interrogante sino también diferentes instancias en el encuentro del espectador ante una imagen. De este modo, nuestra relación con las imágenes lejos está de ser aproblemática, dada por sentada, sino que el sentido de éstas es el resultado de complejas operaciones tanto individuales como sociales.","palabras_clave":"IMAGEN|SENTIDO|FOTOGRAFIA|FENOMENOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4439,"idioma_id":4,"titulo":"A multiproduct batch plant design model incorporating production planning and scheduling decisions under a multiperiod scenario","resumen":"The goal of this work is to propose a multiperiod mixed-integer linear programming model that integrates several decisions about multistage multiproduct batch plants. Usually, plant design was solved without considering operation decisions. The proposed approach considers not only production planning but also scheduling decisions. The time horizon consists of several periods in which deterministic variations in prices, products demand limits, costs, and raw materials availability are taken into account. The plant operates using different production campaigns along each time period. The proposed model allows not only finding the optimal plant structure (unit sizes and its duplication in parallel in each stage), but also the detailed production planning in every time period. In this way, this work enables to assess the trade-offs between the different involved decision levels, taking into account fluctuations along the time horizon.","palabras_clave":"MULTIPERIOD MILP MODEL|MULTIPRODUCT BATCH PALNTS|DESIGN|PRODUCTION PLANNING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4440,"idioma_id":4,"titulo":"Draft whole-genome sequence of Sphingobium sp. 22B,  PAH-degrading bacteria from semi-arid Patagonia, Argentina","resumen":"Sphingobium sp. 22B is a PAH-degrading strain isolated from Patagonia, Argentina, with capabilities to withstand the environmental factors of that semi-arid region. The draft genome shows the presence of genes related with responses to carbon starvation and drying environmental condition.","palabras_clave":"Genome|PAH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4441,"idioma_id":4,"titulo":"Ecological status of a Patagonian mountain river: usefulness of environmental   and biotic metrics for rehabilitation assessment","resumen":"This work evaluates the consequences of anthropogenic pressures at different sections of a Patago-nian mountain river using a set of environmental and bio-logical measures. A map of risk of soil erosion at a basin scale was also produced. The study was conducted at 12 sites along the Percy River system, where physicochemical parameters, riparian ecosystem quality, habitat condition,plants, and macroinvertebrates were investigated. While livestock and wood collection, the dominant activities at upper and mean basin sites resulted in an important loss of the forest cover still the riparian ecosystem remains in arelatively good status of conservation, as do the in-stream habitat conditions and physicochemical features. Besides, most indicators based on macroinvertebrates revealed that both upper and middle basin sections supported similar assemblages, richness, density, and most functional feedinggroup attributes. Instead, the lower urbanized basin showed increases in conductivity and nutrient values, poor quality in the riparian ecosystem, and habitat condition. According to the multivariate analysis, ammonia level, elevation, current velocity, and habitat conditions had explanatorypower on benthos assemblages. Discharge, naturalness of the river channel, flood plain morphology, conservation status, and percent of urban areas were important moder-ators of plant composition. Finally, although the present land use in the basin would not produce a significant risk of soil erosion, unsustainable practices that promotes the substitution of the forest for shrubs would lead to severe consequences. Mitigation efforts should be directed toprotect headwater forest, restore altered riparian ecosys-tem, and to control the incipient eutrophication process","palabras_clave":"Land use|Habitat quality|Riparian ecosystems|Plants|Macroinvertebrates|Forest conservation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4442,"idioma_id":4,"titulo":"Extended and local structural description of a kaolinitic clay, its fired ceramics and intermediates: An XRD and XANES analysis","resumen":"The thermal transformations processes of kaolinite (K) in kaolinitic clays is knownto consist of a dehydroxylationinto metakaolin (MK) at &#8776;600 °C, followed by the formation of a spinel type aluminosilicate (SAS) at&#8776;980 °C,and finally the development of mullite (M) at higher temperatures (1200?1300 °C).The structural characterization of thesematerials is generally based on X-ray diffraction (XRD) studies,where theXRD features of K and M are well defined due to their crystalline nature, but as consequence of the low crystallinityof MK and SAS, the precise characterization of these phases is not possible using this technique.In this study the nature of aluminum atoms in the different materials obtained by thermal treatments of a kaoliniteis investigated using Al K-XANES and compared with other well-known aluminum containing materials.XRD and SEM characterization were also carried out.The results confirm the potentiality of this synchrotron based techniques for the characterization of natural materialsand ceramics especially with low crystallinity. Particularly the mullite aluminums Al K-XANES spectrawere not reported before.","palabras_clave":"CLAY|STRUCTURE|PROPERTIES|XANES|DRX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4443,"idioma_id":4,"titulo":"American Tegumentary Leishmaniasis: T cell differentiation profile of cutaneous and mucosal forms. Co-infection with Trypanosoma cruzi","resumen":"American tegumentary leishmaniasis displays two main clinical forms: cutaneous (CL) and mucosal (ML). ML is more resistant to treatment and displays a more severe and longer evolution. Since both forms are caused by the same Leishmania species, the immunological response of the host may be an important factor determining the evolution of the disease. Herein, we analyzed the differentiation and memory profile of peripheral CD4+ and CD8+ T lymphocytes of patients with CL and ML and their Leishmania?T. cruzi co-infected counterparts. We measured the expression of CD27, CD28, CD45RO, CD127, PD-1 and CD57, together with interferon-&#947; and perforin. A highly differentiated phenotype was reflected on both T subsets in ML and preferentially on CD8+ T cells in CL. A positive trend toward a higher T differentiation profile was found in T. cruzi-infected CL and ML patients as compared with Leishmania single infections. Association between CD8+ T-cell differentiation and illness duration was found within the first year of infection, with progressive increase of highly differentiated markers over time. Follow-up of patients with good response to therapy showed predominance of early differentiated CD8+ T cells and decrease of highly differentiated cells, while patients with frequent relapses presented the opposite pattern. CD8+ T cells showed the most striking changes in their phenotype during leishmaniasis. Patients with long-term infections showed the highest differentiated degree implying a relation between T differentiation and parasite persistence. Distinct patterns of CD8+ T differentiation during follow-up of different clinical outcomes suggest the usefulness of this analysis in the characterization of Leishmania-infected patients.","palabras_clave":"CUTANEOUS LEISHMANIASIS|DIFFERENTIATION AND MEMORY PHENOTYPE|LEISHMANIAT. CRUZI CO-INFECTION|MUCOSAL LEISHMANIASIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4444,"idioma_id":4,"titulo":"Progressive reduction of circulating B lymphocytes in patients with X-linked lymphoproliferative disease (XLP)","resumen":"Inactivating mutations of the SAP\/SH2D1A\/DSHP gene (Nichols et al, 1998) determine the occurrence of human X-linked lymphoproliferative disease (XLP), a condition associated to inappropriate response to Epstein-Barr virus (EBV) infection. XLP patients who survive fulminant infectious mononucleosis and hemophagocytic syndrome, causing 50 % death after initial EBV infection, may develop dysgammaglobulinemia or hypogammaglobulinemia and\/or lymphoproliferative diseases. Inefficient immune response against EBV is unable to control EBV expansion in most cases. Abnormalities in the balance of T helper 1 (Th1)\/ Th2 responses and accumulation of activated CD8+ T lymphocytes have been described (Czar et al, 2001; Belmonte et al, 2007). Concerning B lymphocytes, the percentage of memory B cells (CD27+ B lymphocytes) is reduced in XLP patients surviving primary EBV infection compared to that of normal controls (N) (Malbran et al, 2001). However, somatically mutated IgM+CD27+, but not Ig-subtype switched B cells could be found in XLP despite the absence of germinal centers in secondary lymphoid organs (Coraglia et al, 2010). Likewise, after prolonged culture of peripheral blood mononuclear cells (PBMC), the percentage of residual surviving B lymphocytes having a non-switched memory phenotype (IgM+IgD+CD27+) was higher than N in XLP patients (Belmonte et al, 2009). We have studied the long term (>10 years) evolution of the EBV viral load (EBV VL) and B lymphocyte profile in two surviving hypogammaglobulinemic siblings of an established XLP family (Malbran et al, 2001). One of them (#9) suffered EBV infection at adult age, developed severe EBV-associated disease and was treated with antiviral therapy and with Rituximab (Milone et al, 2005). He recovered from primary EBV infection, became hypogammaglobulinemic and received substitutive monthly intravenous immunoglobulins (IVIg) infusions until 2013, when he presented a bone marrow aplasia and was successfully allotransplanted. The older sibling (#4) developed XLP-related hypogammaglobulinemia after a tonsilar lymphoma at age 4, and remained on gamma globulin treatment for 35 years. Along this time, both patients were periodically evaluated searching for phenotypic and functional changes of both T and B lymphocytes. A predominant terminal effector memory CD8+ T cell phenotype (CD27-CD28-CD8+) was observed in patient #9 when compared to his brother (#4) (Belmonte et al, 2007). In this communication we report the progressive loss of B lymphocytes observed in both patients along the period of study, in coincidence with reduction of the EBV VL. While both patients shared the same genetic defect, their initial encounter with EBV occurred at different ages (childhood in #4 and at adult age #9) and they were treated differently. Patient #9 was 27 years old and had been asymptomatic before his XLP-marker disease (severe acute EBV infection). He had received preventive IgG replacement since he was genetically diagnosed as XLP. His EBV VL was 18164 EBV copies\/106 leukocytes during the acute phase of his disease. At this time he received rituximab treatment and his CD19+ lymphocytes fell to < 0.5 %, coincident with a reduction of the EBV VL to 278. Two years after (Fig 1), the CD19 values started to increase, reaching the normal range six years after rituximab treatment. The EBV VL was still high two years after the acute phase of his disease (8620-8750 EBV copies\/106 leukocytes) and persisted until year five (3969 EBV copies\/106 leukocytes), becoming undetectable after six years (Fig 1). Patient #9 has never had any of the clinical symptoms of chronic active Epstein-Barr virus disease (Cohen et al, 2015). His B lymphocyte values steadily decreased, reaching 3.3-3.4 % with negative EBV VL seven years after his initial EBV-disease. This patient?s clinical condition required an allogeneic bone marrow transplant. This procedure was successfully done in the USA and he recovered his good health. We had no access to study the characteristics of his T and B lymphocytes, since he moved out of Argentina.Patient #4, who had his first XLP related condition at age 2, had low circulating CD19+ cells when we first studied his B lymphocyte profile (2-3 %, 33 years after his XLP-marker disease) (Fig 1). These values decreased further, reaching 0.34 % after 12 years of follow up, when he was 45 years of age. Along this period he continued receiving substitutive monthly IVIg, and his EBV VL remained below 100 EBV copies\/106 leukocytes. As reported before (Malbran et al, 2001), memory CD27+ B lymphocytes were lower than normal in XLP. In both patients this value was relatively stable within the B lymphocyte pool oscillating around 5-14 % of the total B lymphocytes (Fig 1), but when the B lymphocyte percentages fell below 3 % in patient #4, memory B lymphocytes decreased to 0.5 %.  In both patients the absolute B and CD4+ T lymphocyte values were lower (21-95 B lymphocytes\/mm3; 1.60-11.3 memory B lymphocytes\/mm3; 320-500 CD4+ T lymphocytes\/mm3) compared to those of normal controls (72-200 B lymphocytes\/mm3; 28-43 memory B lymphocytes\/mm3; 600-900 CD4+ T lymphocytes\/mm3), while CD8+ T lymphocytes were increased (700-3000 CD8+ T lymphocytes\/mm3 in XLP and 320-700 CD8+ T lymphocytes\/mm3 in N).It is difficult to explain why B lymphocytes were progressively reduced in these XLP patients. Several factors must be taken into account. It is known that germinal center persistence and formation are affected in XLP patients probably because TFH-B contacts are transient in the absence of SAP (Qi et al, 2008). This may lead to impaired B cell memory formation (Malbran et al, 2001; Coraglia et al, 2010). Continuous IgG replacement along many years may alter the balance of circulating B cells and their function. Inhibition of BCR-dependent and BCR-independent antigen presentation by B lymphocytes after IVIg infusion was demonstrated (Paquin Proulx et al, 2010). Also, CD8+ T lymphocytes reacting against EBV infected B lymphocytes could contribute to reduce the B cell count along many years.Replacement treatment with IgG will still be mandatory for the protection of these patients if they do not undergo allogeneic transplantation and recover the balance of their immune system. Anyway, progressive loss of B lymphocytes after many years of XLP evolution must be taken into account as an additional complication of this genetic defect.","palabras_clave":"B LYMPHOCYTES IN XLP|EBV VIRAL LOAD IN XLP|LONG TERM FOLLOW UP IN XLP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4445,"idioma_id":4,"titulo":"Characterization of a double-CRD-mutated Gal-8 recombinant protein that retains co-stimulatory activity on antigen-specific T-cell response","resumen":"Galectins (Gals) constitute a family of mammalian lectins with affinity for &#946;-galactosides, characterized by the presence of conserved CRDs (carbohydrate-recognition domains). We have found previously that Gal-8, from the tandem-repeat group with two linked CRDs, exerts two separate actions on CD4+ T-cells: antigen-independent proliferation and, at lower concentration, antigen-specific co-stimulation. Whereas proliferation can be ascribed to the pro-inflammatory role of Gal-8, the co-stimulatory activity of borderline T-cell-specific responses allows the proposal of Gal-8 as an adjuvant in vaccination. To study the relevance of glycan-lectin interaction to these T-cell activities, we generated a double-mutated protein (Gal-8mut) by replacing canonical arginine residues on each CRD, so as to abolish sugar-binding capacity. As expected, Gal-8mut was unable to bind to lactosyl-Sepharose, confirming that lactose recognition was precluded; however, preservation of lectin activity was still evident since Gal-8mut displayed haemoagglutinatory effects and binding capacity to the T-cell surface. To search for glycan affinity, a glycan microarray analysis was conducted which revealed that Gal-8mut lost most low- and intermediate-, but retained high-, affinity interactions, mainly to polylactosamines and blood group antigens. These findings were supported further by molecular modelling. Regarding biological activity, Gal-8mut was unable to induce T-cell proliferation, but efficiently co-stimulated antigenspecific responses, both in vitro and in vivo. Therefore Gal-8mut represents a useful tool to dissect the specificities of lectin-glycan interactions underlying distinctive Gal-8 activities on T-cell biology. Moreover, given its distinguishing properties, Gal-8mut could be used to enhance borderline immune responses without the non-specific pro-inflammatory activity or other potential adverse effects.","palabras_clave":"GLYCAN-AFFINITY|INFLAMMATION|TCR-COSTIMULATION|T CELL PROLIFERATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4446,"idioma_id":4,"titulo":"Interaction of singlet oxygen with bovine serum albumin and the role of the protein nano-compartmentalization","resumen":"Singlet molecular oxygen(1O2) contributes to protein damage triggering biophysical and biochemical changes that can be related with aging and oxidative stress. Serum albumins,such as bovine serum albumin (BSA), are abundant proteins in blood plasma with different biological functions. This paper presents a kinetic and spectroscopic study of the 1O2-mediated oxidation of BSA using the tris(2,2&#8242;-bipyridine)ruthenium(II) cation [Ru(bpy)3]2þ as sensitizer. BSA quenches efficiently 1O2 with a total(chemicalþphysical interaction) rate constant kt BSA¼7.3(70.4)  108 M1 s1, where the chemical pathway represented 37% of the interaction. This efficient quenching by BSA indicates the participation of several reactive residues. MALDI-TOF MS analysis of intact BSA confirmed that after oxidation by 1O2, the mass protein increased the equivalent of 13 oxygen atoms. Time-resolved emission spectra analysis of BSA established that Trp residues were oxidized to N&#8242;-formylkynurenine, being the solvent-accessibleW134 preferentially oxidized by 1O2 as compared with the buried W213. MS confirmed oxidation of at least two Tyr residues to form dihydroxyphenylalanine, with a global reactivity towards 1O2 six-times lower than for Trp residues. Despite the lack of MS evidences, kinetic and chemical analysis also suggestedthat residues other than Trp and Tyr, e.g. Met, must react with 1O2. Modeling of the 3D-structure of BSA indicated that the oxidation pattern involves a random distribution of 1O2 into BSA; allowing also the interaction of 1O2 with buried residues by its diffusion from the bulk solvent through interconnectedinternal hydrophilic and hydrophobic grooves.","palabras_clave":"TRYPTOPHAN FLUORESCENCE|SINGLET OXYGEN|REACTIVE OXYGEN SPECIES|PROTEIN OXIDATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4447,"idioma_id":4,"titulo":"Temporal and spatial expression of Muc2 and Muc5ac mucins during rat respiratory and digestive tracts development","resumen":"Secreted mucins constitute a crucial part of the gel that protects respiratory and digestive epithelia, being MUC2\/Muc2 the predominant gel-forming mucin of the intestine while MUC5AC\/Muc5ac is one of the gel-forming mucins most expressed at the airways. In this study, we have analyzed Muc2 and Muc5ac during rat development by using immunohistochemistry, Western blotting and RT-PCR. We demonstrated that rat Muc2 was expressed in fetal intestinal goblet cells of surface epithelium of villi and developing Lieberkühn crypts. In neonates and adults, Muc2 was expressed at luminal goblet cells ofsmall and large intestine and at gastric mucous and glandular cells. Muc5ac protein was observed in embryonic gastric and lung samples; expression increased during development and postnatal and adult life. After birth, a low reaction was detected at the tracheal surface epithelium and glands, which increased in adults.","palabras_clave":"Muc2|Muc5ac|rat|embriology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4449,"idioma_id":4,"titulo":"Homogenization and Truncation processes in Inflorescences of Cyperaceae","resumen":"This work presents an analysis of the homogenization and truncation processesin Cyperaceae inflorescences. The truncation process is rare in Cyperaceae.There may be homogenized inflorescences but in general these inflorescences present aterminal spikelet. In the few studied cases of truncated inflorescences in Cyperaceae thetruncation process may affect the terminal spikelet, only on the main axis and on thelong primary branches (species of Cariceae, species of Lipocarpha and Ascolepis), or itmay also affect the short branches on the main axis (some species of Kobresia; Cyperusgiganteus, C. papyrus and C. prolifer) and on the primary long branches (Cyperusgiganteus, C. papyrus and C. prolifer). The homogenized inflorescences can bedisjunct or not disjunct. The disjunct homogenized inflorescences have a distal portionof short branches and a proximal portion of long branches, which lead in turn as shortbranches subunits. Meanhile non-disjunct homogenized inflorescences have only shortor long branches, all with the same branching degree.","palabras_clave":"INFLORESCENCES|TRUNCATION|HOMOGENIZATION|CYPEARCEAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4450,"idioma_id":4,"titulo":"Contrasting Plasticity in Ovariole Number Induced by A Dietary Effect of the Host Plants between Cactophilic Drosophila Species","resumen":"Under the preference-performance hypothesis, natural selection will favor females that choose oviposition sites that optimize the fitness of their offspring. Such a preference-performance relationship may entail important consequences mainly on fitness-related traits. We used the well-characterized cactus-Drosophila system to investigate the reproductive capacity in the pair of sibling species D. buzzatii and D. koepferae reared in two alternative host plants. According to our hypothesis, ovariole number (as a proxy of reproductive capacity) depends on host plant selection. Our results indicate that the capacity of D. buzzatii showed to be mild, only increasing the number of ovarioles by as much as 10% when reared in its preferred host. In contrast, D. koepferae exhibited a similar reproductive capacity across host cacti, even though it showed a preference for its primary host cactus. Our study also revealed that D. buzzatii has a larger genetic variation for phenotypic plasticity than its sibling, although ovariole number did not show clear-cut differences between species. We will discuss the weak preference-performance pattern observed in these cactophilic species in the light of nutritional and toxicological differences found between the natural host plants.","palabras_clave":"phytophagous insects|developmental plasticity|female reproductive capacity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4451,"idioma_id":4,"titulo":"Diacylglycerol levels modulate the cellular distribution  of the nicotinic acetylcholine receptor","resumen":"Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1\/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1\/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30 min-4 h) whereas at longer times (18 h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4 h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.","palabras_clave":"ACETYLCHOLINE RECEPTOR|DIACYLGLYCEROL|LIPID-PROTEIN INTERACTIONS|PROTEIN KINASE C"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4452,"idioma_id":4,"titulo":"Quantitative trait loci for Fusarium and Gibberella ear rot resistance in Argentinian maize germplasm","resumen":"Fusarium verticillioides and F. graminearumcause ear rots in maize (Zea mays L.) thatreduce yield and contaminate the grain with mycotoxinsproduced by the fungi. To map QTLs forresistance to these ear rots, a F5 mapping population,consisting of 298 recombinant inbreds obtained byrandomly selfing of the cross between LP4637 (moderatelyresistant) and L4674 (susceptible), was genotypedwith 250 single nucleotide polymorphismmarkers and phenotyped 2 years for disease severityafter silk inoculation with conidial suspensions of F.verticillioides and F. graminearum. Four QTLs weremapped in chromosomes 2, 3 and 5, bins 2.03, 3.05,3.07 and 5.07, explaining ranges of 11.2?11.8,3.4?5.1, 6.2?7.6 and 3.8?5.0 of phenotypic variances(%), respectively, depending on year and fungus.Additive effects of each QTL ranged from 5.0 to11.9 % of ear area covered by mold and no epistaticinteractions were observed. The four QTLs wereeffective for both Fusarium species and environmentsindicating that LP4637 is a source of broad resistanceto Fusarium stable across environments. These resultsare consistent with previous research reporting QTLsfor ear rot resistance in the same chromosome regionsfrom sources of resistance growing in North America,Africa, Europe and China.","palabras_clave":"maiz|Fusarium verticillioides|Fusarium graminearum|QTL quantitative trait loci"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4453,"idioma_id":4,"titulo":"Understanding the role of nickel on the hydrogen storage capacity of Ni\/MCM-41 materials","resumen":"In this study, we prepared MCM-41 mesoporous materials modified with different Ni contents, as promising materialsfor hydrogen storage. We focused on the role of dispersed nickel as a way of promoting the interaction with hydrogen asan alternative to improve hydrogen storage materials. In addition, the reducibility of Ni\/MCM-41 materials was studied,and the hydrogen adsorption in the reduced materials was evaluated, in order to compare the behaviour of reduced andunreduced Ni\/MCM-41 materials in hydrogen storage at 77 and 293 K. Hydrogen adsorption isotherms were measuredat 77 K and 293 K, and at low and high-pressure conditions respectively. The results obtained show that the amount ofhydrogen stored was highly enhanced by the dispersion of small amounts of Ni nanoparticles both at 77 and 293 K.Experimental techniques such as TPR, XPS, N2 adsorption-desorption and computational methods (DFT) were usedin order to interpret the results and contribute to the understanding of hydrogen adsorption on Ni\/MCM-41 materials.","palabras_clave":"DFT|Nickel|MCM-41|HYDROGEN STORAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4454,"idioma_id":4,"titulo":"Cohesive surface model for fracture based on a two-scale formulation: computational implementation aspects","resumen":"The paper describes the computational aspects and numerical implementation of a two-scale cohesive surface methodology developed for analyzing fracture in heterogeneous materials with complex microstructures. This approach can be categorized as a semi-concurrent model using the Representative Volume Element (RVE) concept.A variational multi-scale formulation of the methodology has been previously presented by the authors. Subsequently, the formulation has been generalized and improved in two aspects: i) cohesive surfaces have been introduced at both scales of analysis, they are modeled with a strong discontinuity kinematics (new equations describing the insertion of the macro-scale strains, into the micro-scale and the posterior homogenization procedure have been considered); ii) the computational procedure and numerical implementation have been adapted for this formulation. The first point has been presented elsewhere, and it is summarized here. Instead, the main objective of this paper is to address a rather detailed presentation of the second point.Finite element techniques for modeling cohesive surfaces at both scales of analysis (FE2 approach) are described: i) finite elements with embedded strong discontinuities (EFEM) are used for the macroscale simulation, and ii) continuum-type finite elements with high aspect ratios, mimicking cohesive surfaces, are adopted for simulating the failure mechanisms at the micro-scale.The methodology is validated through numerical simulation of a quasi-brittle concrete fracture problem. The proposed multi-scale model is capable of unveiling the mechanisms that lead from the material degradation phenomenon at the meso-structural level to the activation and propagation of cohesive surfaces at the structural scale.","palabras_clave":"multi-scale cohesive models|computational homogenization|heterogeneous material failure|Embedded Finite Elements (EFEM)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4455,"idioma_id":4,"titulo":"Influence of heat pre-treatment on BSA tryptic hydrolysis and peptide release","resumen":"In contrast with other food proteins, such as b-lactoglobulin or caseins, intensely studied for bioactivepeptide production, relatively little attention has been paid to serum albumin, the main blood protein,even though blood disposal is a severe problem for meat processors. In this study, serum albumin washydrolysed with trypsin after several heat treatments and using different enzyme concentrations. Thedegree of hydrolysis reached and the peptide sequences released over time were evaluated. Large differencesin enzyme-to-substrate ratios (1:50, 1:100 and 1:200) led to similar degree of hydrolysis values(31.92 ± 1.43%, 31.08 ± 3.09% and 26.21 ± 0.71%), and did not alter the number of peptides released.However, thermal treatment enhanced significantly (p < 0.05) both the degree of hydrolysis (up to50.41 ± 1.90%) and the number and amount of the majority of peptides obtained, all with potential bioactivity(28 peptides in the native hydrolysate, 39 in the thermally treated).","palabras_clave":"BOVINE SERUM ALBUMIN|BIOACTIVE PEPTIDES|HYDROLYSIS|HEAT TREATMENT|MEAT BY-PRODUCTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4456,"idioma_id":4,"titulo":"A quantitative model for oxygen uptake and release in a family of hemeproteins","resumen":"Motivation: Hemeproteins have many diverse functions that largely depend on the rate at which they uptake or release small ligands, like oxygen. These proteins have been extensively studied using either simulations or experiments, albeit only qualitatively  and one or two proteins at a time.Results: We present a physical?chemical model, which uses data obtained exclusively from computer simulations, to describe the uptake  and release of oxygen in a family of hemeproteins, called truncated hemoglobins (trHbs). Through a rigorous statistical analysis we demonstrate that our model successfully recaptures all the reported experimental oxygen association and dissociation kinetic rate constants, thus allowing us to establish the key factors that determine the rates at which these hemeproteins uptake and release oxygen. We found that internal tunnels as well as the distal site water molecules control ligand uptake, whereas oxygen stabilization by distal site residues controls ligand release. Because these rates largely determine the functions of these hemeproteins, these approaches will also be important tools in characterizing the trHbs members with unknown functions.","palabras_clave":"hemeproteins|uptake and release oxygen|truncated hemoglobins |association and  dissociation kinetic rate constants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4457,"idioma_id":4,"titulo":"Properties of Magnetic tongues over a Solar Cycle","resumen":"The photospheric spatial distribution of the main magnetic polaritiesof bipolar active regions (ARs) presents during their emergence deformations are known as magnetic tongues. They are attributed to the presence of twist in the toroidal magnetic flux-tubes that form the ARs. The aim of this article is to study the twist of newly emerged ARs from the evolution of magnetic tongues observed in photospheric line-of-sight magnetograms. We apply the procedure described by Poisson et al. (2015, Solar Phys. 290, 727) to ARs observed over the full Solar Cycle 23 and the beginning of Cycle 24. Our results show that the hemispherical rule obtained using the tongues as a proxy of the twist has a weak sign-dominance (53 % in the southern hemisphere and 58 % in the northern hemisphere). By defining the variation of the tongue angle, we characterize the strength of the magnetic tongues during different phases of the AR emergence. We find that there is a tendency of the tongues to be stronger during the beginning of the emergence and to become weaker as the AR reaches its maximum magnetic flux. We compare this evolution with the emergence of a toroidal flux-rope model with non-uniform twist. The variety of evolution of the tongues in the analyzed ARs can only be reproduced when using a broad range of twist profiles, in particular having a large variety of twist gradient in the direction vertical to the photosphere. Although the analytical model used is a special case, selected to minimize the complexity of the problem, the results obtained set new observational constraints to theoretical models of flux-rope emergence that form bipolar ARs.","palabras_clave":"ACTIVE REGIONS: MAGNETIC FIELDS|CORONA: STRUCTURES|HELICITY: MAGNETIC |HELICITY: OBSERVATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4458,"idioma_id":4,"titulo":"Evolutionary and Functional Relationships in the Truncated Hemoglobin Family","resumen":"Predicting function from sequence is an important goal in current biological research, and although, broad functional assignment is possible when a protein is assigned to a family, predicting functional specificity with accuracy is not straightforward. If function is provided by key structural properties and the relevant properties can be computed using the sequence as the starting point, it should in principle be possible to predict function in detail. The truncated hemoglobin family presents an interesting benchmark study due to their ubiquity, sequence diversity in the context of a conserved fold and the number of characterized members. Their functions are tightly related to O2 affinity and reactivity, as determined by the association and dissociation rate constants, both of which can be predicted and analyzed using in-silico based tools. In the present work we have applied a strategy, which combines homology modeling with molecular based energy calculations, to predict and analyze function of all known truncated hemoglobins in an evolutionary context. Our results show that truncated hemoglobins present conserved family features, but that its structure is flexible enough to allow the switch from high to low affinity in a few evolutionary steps. Most proteins display moderate to high oxygen affinities and multiple ligand migration paths, which, besides some minor trends, show heterogeneous distributions throughout the phylogenetic tree, again suggesting fast functional adaptation. Our data not only deepens our comprehension of the structural basis governing ligand affinity, but they also highlight some interesting functional evolutionary trends.","palabras_clave":"Structure Function Prediction|Molecular Dynamics|Molecular Evolution|Haemoglobin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4459,"idioma_id":4,"titulo":"Frequency thermal response and cooling performance in a microscopic system with a time-dependent perturbation","resumen":"Following the nonequilibrium Green´s function formalism we study the thermaltransport in a composite chain subject to a time-dependent perturbation.The system is formed by two nite linear asymmetric harmonic chains subject toan on-site potential connected together by a time-modulated coupling. The endsof the chains are coupled to two phononic reservoirs at dierent temperatures.We present the relevant equations used to calculate the heat current along eachsegment. We nd that the system presents dierent transport regimes accordingthe driving frequency and temperature gradients. One of the regimes correspondsto a heat pump against thermal gradient, thus a characterization of the coolingperformance of the device is presented.","palabras_clave":"Heat conduction |Transport processes|Quantum refrigeration"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4460,"idioma_id":5,"titulo":"ESTUDIOS PETROGRÁFICOS Y DE CORTES DELGADOS PARA LA IDENTIFICACIÓN DE MATERIAS PRIMAS LÍTICAS EN EL SECTOR NORTE DEL VALLE DE HUALFÍN (CATAMARCA, ARGENTINA)","resumen":"El objetivo del siguiente trabajo se centraen el análisis macroscópico y en estudios petrográficosmicroscópicos realizados sobre materialeslíticos arqueológicos recuperados de los sitios Villavily Villavil 2 (Hualfín, Catamarca, Argentina). Losresultados obtenidos permitieron identificar las materiasprimas utilizadas en la manufactura de artefactosasociados a ocupaciones del periodo de DesarrollosRegionales\/Inka (ca. 900 al 1400 d. C.) y,en base a la información geológica de la zona, serealizaron observaciones acerca de su posible procedencia.Esto permitió discutir las hipótesis planteadascon anterioridad acerca de las prácticas relacionadascon su aprovisionamiento","palabras_clave":"TECNOLOGIA LÍTICA|MATERIAS PRIMAS|ANÁLISIS PETROGRÁFICO|VALLE DE HUALFÍN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4461,"idioma_id":4,"titulo":"Development of innovative biodegradable films based on biomass of Saccharomyces cerevisiae","resumen":"Biodegradable alternatives to petroleum-based polymers are being developed for food packaging. Saccharomyces cerevisiae has been widely used for the production of fermented beverages and leavened foods. In this work, the application of high pressure homogenisation and a thermal treatment to the yeast biomass was studied to develop biodegradable films. The highest dispersibility indexes of protein (84.5 ± 3.3%), carbohydrates (24.3 ± 1.1%), RNA (40.6 ± 0.9%) and soluble solids released were found at high homogenisation pressure (125 MPa). Combinations of one or two homogenisations and a thermal treatment at 90 °C during 20 minutes were applied and dispersions were fully characterised, focusing on their film-forming capacity. The best combination was homogenisation, heat treatment and a further homogenisation, since it produced films that presented good attributes, great continuity and homogeneity without small cracks. However, hydration of films was increased from 0.31 to 0.48 gH2O\/g.d.m with the number of homogenisation processes applied. Results revealed that yeast biomass is a viable source to be used in biodegradable films.","palabras_clave":"HIGH PRESSURE HOMOGENISATION|YEAST|SACCHAROMYCES CEREVISIAE|BIODEGRADABLE FILM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4462,"idioma_id":5,"titulo":"CARACTERIZACIÓN QUÍMICA MINERAL DE LAS ROCAS MÁFICAS DE LA SIERRA DE LA INVERNADA, PRECORDILLERA CENTRAL, SAN JUAN","resumen":"Las rocas máficas de la sierra de la Invernada corresponden a gabros ricos en plagioclasa cálcica (bitownita), clinopiroxeno (augita y pigeonita), escaso anfíbol y minerales secundarios característicos de facies de esquistos verdes. La composición química mineral de los clinopiroxenos permite clasificar estas rocas como basaltos tolehíticos anorogénicos y de fondo oceánico, compartiendo un origen común con rocas máficas y ultramáficas de la Precordillera Occidental, que separan los terrenos Chilenia y Cuyania.","palabras_clave":"PRECORDILLERA OCCIDENTAL|THOLEITAS ANOROGENICAS|GABROS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4463,"idioma_id":4,"titulo":"Mifepristone increases mRNA translation rate, triggers the unfolded protein response, increases autophagic flux, and kills ovarian cancer cells in combination with proteasome or lysosome inhibitors","resumen":"The synthetic steroid mifepristone blocks the growth of ovarian cancer cells, yet the mechanism driving such effect is not entirely understood. Unbiased genomic and proteomic screenings using ovarian cancer cell lines of different genetic backgrounds and sensitivities to platinum led to the identification of two key genes upregulated by mifepristone and involved in the unfolded protein response (UPR): the master chaperone of the endoplasmic reticulum (ER), glucose regulated protein (GRP) of 78 kDa, and the CCAAT\/ enhancer binding protein homologous transcription factor (CHOP). GRP78 and CHOP were upregulated by mifepristone in ovarian cancer cells regardless of p53 status and platinumsensitivity. Further studies revealed that the three UPR-associated pathways, PERK, IRE1a, and ATF6, were activated by mifepristone. Also, the synthetic steroid acutely increased mRNA translation rate, which, if prevented, abrogated the splicing of XBP1 mRNA, a non-translatable readout of IRE1a activation. Moreover, mifepristone increased LC3-II levels due to increased autophagic flux. When the autophagicelysosomal pathway was inhibited with chloroquine, mifepristone was lethal to the cells. Lastly, doses of proteasome inhibitors that are inadequate to block the activity of the proteasomes, caused cell death when combined with mifepristone; this phenotype was accompanied by accumulationof poly-ubiquitinated proteins denoting proteasome inhibition. The stimulation by mifepristone of ER stress and autophagic flux offers a therapeutic opportunity for utilizing this compound to sensitize ovarian cancer cells to proteasome or lysosome inhibitors.","palabras_clave":"OVARIAN CANCER|BORTEZOMIB|MIFEPRISTONE|CHLOROQUINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4464,"idioma_id":4,"titulo":"Effects of 2,4-dichlorophenoxyacetic acid on the ventral prostate of rats during the peri-pubertal, pubertal and adult stage","resumen":"The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is used on a wide variety of terrestrial and aquatic broadleaf weeds. 2,4-D has been shown to produce a wide range of adverse effects on animal and human health. The aim of the current study was to evaluate the effects of pre- and postnatal exposure to 2,4-D on rat ventral prostate (VP). Pregnant rats were exposed daily to oral doses of 70&#8201;mg\/kg\/day of 2,4-D from 16 days of gestation up to 23 days after delivery. Then, the treated groups (n&#8201;=&#8201;8) were fed with a 2,4-D added diet until sacrificed by decapitation on postnatal day (PND) 45, 60, or 90. Morphometric studies were performed and androgen receptor (AR) protein levels in the VP were determined. AR, insulin-like growth factor-I (IGF-1) and insulin-like growth factor-I receptor (IGF-1R) mRNA expression in the VP along with testosterone (T), dihydroxytestosterone (DHT), growth hormone (GH) and IGF-1 serum levels were also determined to ascertain whether these parameters were differentially affected. Results of this study showed that 2,4-D exposure during gestation and until adulthood altered development of the prostate gland in male rats, delaying it at early ages while increasing its size in adults, indicate that 2,4-D could behave as endocrine disruptors (EDs).","palabras_clave":"2,4-dichlorophenoxyacetic acid|prostata|rata|toxicologia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4465,"idioma_id":4,"titulo":"Can We Improve Nitrogen Management for Sunflower?","resumen":"Nitrogen application in sunfl ower would increase seedyield and seed protein concentration without affecting seedoil concentration. Moreover, NA (PPSNT + FN) allowed thedetermination of optimum N availability for seed yield andmaximized seed protein concentration, which would increasethe quality of the fl our and pellets. Meanwhile, the SPADmeasurements have proved to be a tool that could complementsoil N diagnosis and requires further investigation forpractical use.","palabras_clave":"GIRASOL|ACEITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4466,"idioma_id":4,"titulo":"AhR ligand Aminoflavone inhibits &#945;6-integrin expression and breast cancer sphere-initiating capacity.","resumen":"Traditional chemotherapies debulk tumors but fail to produce long-term clinical remissions due to their inability to eradicate tumor-initiating cells (TICs). This necessitates therapy with activity against the TIC niche. &#913;lpha6-integrin (&#945;6-integrin) promotes TIC growth. In contrast, aryl hydrocarbon receptor (AhR) signaling activation impedes the formation of mammospheres (clusters of cells enriched for TICs). We investigated the ability of AhR agonist Aminoflavone (AF) and AF pro-drug (AFP464) to disrupt mammospheres derived from breast cancer cells and a M05 mammary mouse model of breast cancer respectively. We further examined the capacity of AF and AFP464 to exhibit anticancer activity and modulate the expression of 'stemness' genes including &#945;6-integrin using immunofluorescence, flow cytometry and qRT-PCR analysis. AF disrupted mammospheres and prevented secondary mammosphere formation. In contrast, AF did not disrupt mammospheres derived from AhR ligand-unresponsive MCF-7 cells. AFP464 treatment suppressed M05 tumor growth and disrupted corresponding mammospheres. AF and AFP464 reduced the expression and percentage of cells that stained for 'stemness' markers including &#945;6-integrin in vitro and in vivo respectively. These data suggest AFP464 thwarts bulk breast tumor and TIC growth via AhR agonist-mediated &#945;6-integrin inhibition.","palabras_clave":"Alpha integrin 6|Aminoflavone|Aryl hydrocarbon receptor|Mammospheres"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4467,"idioma_id":4,"titulo":"Involvement  of TLR6 in the induction of COX-2, PGE2 and IL-10 in macrophages by lipids from virulent S2P and attenuated R1A Babesia bovis strains","resumen":"Toll like receptors (TLRs) are involved in the modulation of diverse host genes expression through a complexnetwork of signalling events that allow for an appropriate response to a microbial pathogen. In thepresent work we used TLR6KO mice in order to study the role of TLR6 in the immune discriminationof lipids from two Babesia bovis strains, attenuated R1A (LA) and virulent S2P (LV), and the consequentmacrophage activation. We demonstrated that TLR6 is required for lipid body induction in murine peritonealmacrophages by both LA and LV. Interestingly, as regards IL-10 and COX-2\/PGE2 pathway inductionby LA and LV, we observed differences in the biological effects produced by these lipid extracts. Our resultsindicate a role of TLR6 in the down-modulation of these immunoregulators only in the case of LA, whereasthis receptor was not implicated in pro-inflammatory TNF, IL-6 and KC release induced by LA. Remarkably,LV did not exert the down-modulatory effect observed for LA, supporting the notion that LA and LVpossess different lipid composition that could correlate with the polar pathogenic effect of both B. bovisstrains.","palabras_clave":"Lipids| Babesia bovis |TLR6 |Macrophages"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4468,"idioma_id":4,"titulo":"Regulation of RNA binding proteins in trypanosomatid protozoan parasites","resumen":"Posttranscriptional mechanisms have a critical role in the overall outcome of gene expression. These mechanisms are especially relevant in protozoa from the genus Trypanosoma, which is composed by death threatening parasites affecting people in Sub-saharan Africa or in the Americas. In these parasites the classic view of regulation of transcription initiation to modulate the products of a given gene cannot be applied. This is due to the presence of transcription start sites that give rise to long polycistronic units that need to be processed costranscriptionally by trans-splicing and polyadenylation to give mature monocistronic mRNAs. Posttranscriptional mechanisms such as mRNA degradation and translational repression are responsible for the final synthesis of the required protein products. In this context, RNA-binding proteins (RBPs) in trypanosomes have a relevant role as modulators of mRNA abundance and translational repression by associating to the 3' untranslated regions in mRNA. Many different RBPs have been proposed to modulate cohorts of mRNAs in trypanosomes. However, the current understanding of their functions lacks a dynamic view on the different steps at which these RBPs are regulated. Here, we discuss different evidences to propose regulatory events for different RBPs in these parasites. These events vary from regulated developmental expression, to biogenesis of cytoplasmic ribonucleoprotein complexes in the nucleus, and condensation of RBPs and mRNA into large cytoplasmic granules. Finally, we discuss how newly identified posttranslational modifications of RBPs and mRNA metabolism-related proteins could have an enormous impact on the modulation of mRNA abundance. To understand these modifications is especially relevant in these parasites due to the fact that the enzymes involved could be interesting targets for drug therapy.","palabras_clave":"TRYPANOSOMATIDS|RNA|REGULATION|TRANSLATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4469,"idioma_id":4,"titulo":"Antifouling activity of green-synthesized 7-hydroxy-4- methylcoumarin","resumen":"In the search for new environmental-friendly antifoulants for replace metallic biocides, 7-hydroxy-4-methylcoumarin was synthesized according to green chemistry procedures. This compound was characterizedby current organic analysis and its antifouling properties were firstly evaluated on the bivalveMytilus edulis platensis in the laboratory. In the second stage, a soluble matrix antifouling coatingformulated with this compound was assayed in marine environment. Laboratory experiments showedthat 7-hydroxy-4-methylcoumarin was effective in inhibiting both the settlement as well as the byssogenesisof mussels. In addition, after exposure time in the sea, painted panels containing this compoundshowed strong antifouling effect on conspicuous species of the fouling community of Mar el Plata harbor.In conclusion, green-synthesized coumarin could be a suitable antifoulant candidate for marine protectivecoatings.","palabras_clave":"Biofouling|Antifouling coating|Green ChemistryCoumarin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4470,"idioma_id":4,"titulo":"Tungstophosphoric acid supported on core-shell polystyrene-silica microspheres or hollow silica spheres catalyzed trisubstituted imidazole synthesis by multicomponent reaction","resumen":"tMaterials based on tungstophosphoric acid supported on core-shell polystyrene-silica microspheres orhollow silica spheres were prepared, characterized and used as catalysts in the synthesis of trisubstitutedimidazoles by a multicomponent reaction under solvent-free conditions.For the preparation of the two different silica-based structures, silica was synthesized using tetraethy-lorthosilicate (TEOS) as precursor in an ethanol-ammonium solution and polystyrene spheres as templateor core. The formation and growth of the silica layer to obtain the core-shell microspheres were followedby 24 h. Then, the hollow spheres were obtained by calcination of the core-shell material at 500&#9702;C for3 h. Both supports were impregnated with tungstophosphoric acid (TPA) solutions.A smooth appearance of the spheres was observed by scanning electron microscopy (SEM). Mesoporousmaterials were obtained, without important microporosity, as determined from N2adsorption-desorption isotherms. The Fourier transform infrared (FT-IR) spectra confirmed that complete removal ofthe polystyrene core can directly be achieved by calcination at 500&#9702;C, and also that Keggin undegradedTPA species are present in the impregnated solids. The acidic characteristics of the solids were evaluatedby potentiometric titration, showing that they exhibit very strong acid sites. Moreover, peaks assignedto crystalline forms of TPA were observed by XRD.The yields obtained in the solvent-free synthesis of 2,4,5-triphenyl-1H-imidazole and other eighttrisubstituted imidazoles were high, without formation of by-products resulting from competitive reac-tions or decomposition products, so the prepared materials are highly selective catalysts.","palabras_clave":"Hollow silica spheres|Polystyrene-silica spheres|Tungstophosphoric acid|Trisubstituted imidazolesa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4471,"idioma_id":4,"titulo":"Synthesis, characterization and catalytic evaluation of H3PW12O40 included in acrylic acid\/acrylamide polymer for the selective oxidation of sulfides","resumen":"A series of hybrid materials (SAPTPA) based on tungstophosphoric acid H3PW12O40 (TPA) included in acrylic acid\/acrylamide polymer (SAP) were synthetized. The samples were characterized by FT-IR, XRD, 31P MAS-NMR, DTA-TGA, and the acidic properties were determined by means of potentiometric titration with n-butylamine. Materials with 10, 20, 30 and 40% (w\/w) TPA content in the solid (SAPTPA10, SAPTPA20, SAPTPA30 and SAPTPA40, respectively) were prepared by impregnating the polymer with tungstophosphoric acid water solutions. According to FT-IR and 31P MAS-NMR studies, the main species present in the samples is the [PW12O40]3&#8722; anion, which was partially transformed into [P2W21O71]6&#8722; and [PW11O39]7&#8722; anions during the synthesis and drying steps.Taking into account XRD results, these species are highly dispersed in the polymer matrix or appear as noncrystalline phases. The DTA-TGA results show that SAPTPA materials did not undergo any remarkable chemical changes up to 200 °C. Additionally, the SAPTPA materials showed strong acid sites whose number increased as the TPA content in them was higher.The materials obtained by impregnation of TPA onto the polymer matrix present suitable physicochemical properties to be used as catalysts in the selective oxidation of sulfides to the corresponding sulfoxide\/sulfone using acetonitrile as solvent and H2O2 35% w\/v as a clean oxidant.The SAPTPA40 catalyst was appropriate for the selective sulfide oxidation in heterogeneous conditions and can be reused without significant loss of its catalytic activity. A convenient and selective procedure for oxidizing sulfides to sulfoxides or sulfones by varying the H2O2\/substrate ratio and the temperature was found.","palabras_clave":"Keggin heteropolyacid |Superporous polymers|Sulfoxide |Sulfone"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4472,"idioma_id":4,"titulo":"Genetic variation in natural populations of Acacia visco  (Fabaceae) belonging to two sub-regions of Argentina using AFLP.","resumen":"Abstract Acacia visco is a tree native to South Americathat grows in central and northwest region of Argentina,north Chile and Bolivia and has also been introduced toAfrica and naturalized in Europe. Little is known aboutgenetic diversity and genetic structure of this species.Therefore, we studied natural Argentineanpopulations ofA.visco using the AFLPtechnique, by determining the geneticdiversity ofthe species and its genetic structure, consideringthe proportion of the species diversity explained within andbetween the two sub-regions where populations were thisspecies is present in the country. Fourteen of the 445 lociobtained appeared to be under diversifying selection. Theremaining 431 neutral loci showed a mean of 60. 89 % ofpercentage of polymorphic loci. The estimates of geneticdiversity HE were generally high. The FST (0. 126) washighly signi&#64257;cant, providing evidence for genetic structureamong populations. Hierarchical AMOVA indicated thatvariation between sub-regions was 2. 1 % and highly sig-ni&#64257;cant. The higher component of variance was foundwithin populations (77. 4 %). STRUCTURE analysisshowed an optimal number of K = 6. This result wasconsistent with those obtained by UPGMA from Nei? sdistances and Canonical Discriminant Analysis. Since dif-ferentiation of A. visco populations in sub-regions washighly signi&#64257;cant, a suitable management strategy for theuse of this species in restoration programs would be focuson sampling seeds of a high number of individual treeswithin populations and also ensure a comprehensive cov-erage of the entire ecological amplitude of this species.","palabras_clave":"AFLP|AMOVA|GENETIC DIFFERENTIATION|SENEGALIA VISCO|STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4473,"idioma_id":4,"titulo":"Properties and antioxidant activity of soy protein concentrate films incorporated with red grape extract processed by casting and compression molding","resumen":"Self-standing transparent soy protein concentrate (SPC) films plasticized by glycerol (30% w\/w SPC dry basis) and supplemented with red grape extract (RGE) as natural antioxidant (0e10% w\/w SPC dry basis) were prepared by two methods: casting and intensive mixing followed by compression molding. The influence of RGE on key film properties was analyzed at the light of the specific stabilizing interactions of SPC films. The addition of RGE had a favorable effect on moisture content, total soluble matter, water vapor permeability and percentage of elongation of casted films compare with compression molded counterparts. RGE induced a redistribution of hydrogen interactions of casted films replacing protein protein hydrogen interactions by protein-polyphenol ones with no variations in disulfide bridges, while these last interactions were significantly reduced in compression molded films in favor of hydrophobicand hydrogen interactions, as disclosed by differential solubility assays and infrared spectroscopy. The antioxidant activity of the SPC films in terms of scavenging activity of the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power, increased significantlyirrespective of the manufacturing method, being the release of antioxidants from casted films lower than that from compression molded films in accordance with the strong interactions between SPC matrix and polyphenols.","palabras_clave":"ACTIVE PACKAGING|ANTIOXIDANT|BIODEGRADABLE FILMS|BARRIER PROPERTIES|MECHANICAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4474,"idioma_id":4,"titulo":"Effective participation of Li 4 (NH 2 ) 3 BH 4  in the dehydrogenation  pathway of the Mg(NH 2 ) 2 -2LiH composite","resumen":"Lithium fast-ion conductors have shown positive effects on the hydrogen storage properties of the Li-Mg-N-H system. In the present work, Li4(NH2)3BH4 doped Mg(NH2)2-2LiH was formed by milling the 2LiNH2-MgH2-0.2LiBH4 composite and posterior annealing under hydrogen pressure to reduce the kinetic barrier of the Li-Mg-N-H system. The effect of repetitive dehydrogenation\/rehydrogenation cycles on the kinetic and thermodynamic performance was evaluated. The dehydrogenation rate in the doped composite was twice that in the un-doped sample at 200 °C, while hydrogenation was 20 times faster. The activation energy decreases by 9% due to the presence of Li4(NH2)3BH4 compared to the un-doped composite, evidencing its catalytic role. The presence of Li4(NH2)3BH4 in the composite stabilized the hydrogen storage capacity after successive sorption cycles. Thermodynamic studies revealed a variation in the pressure composition isotherm curves between the first dehydrogenation cycle and the subsequent. The Li4(NH2)3BH4 doped composite showed a sloped plateau region at higher equilibrium pressure in regard to the flat plateau of the un-doped composite. Detailed structural investigations revealed the effective influence of Li4(NH2)3BH4 in different reactions: the irreversible dehydrogenation in the presence of MgH2 and the reversible hydrogen release when it reacts with Li2Mg2(NH)3. The role of Li4(NH2)3BH4 in improving the dehydrogenation kinetics is associated with the weakening of the N-H bond and the mobile small ion mass transfer enhancement.","palabras_clave":"HYDROGEN STORAGE|AMIDES|CATALYST|KINETICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4475,"idioma_id":4,"titulo":"Decomposition of cattle manure and colonization by macroinvertebrates in sediment of a wetland of the Middle Paraná River","resumen":"Purpose The study was carried out in a wetland of the MiddleParaná River system, Argentina, in order to evaluate the processesassociated with the decomposition of manure, whichincludes changes in chemical composition, nutrient release ofmanure, and colonization of invertebrates. We also comparedthe invertebrate assemblage that colonized manure with thatpresent in the benthos of the wetland.Materials and methods Nylon bags were filled with fresh cattlemanure and anchored to the littoral zone of the wetland. Sixbags were collected after 1, 2, 6, 14, 21, 28, 33, 55, and79 days: three for invertebrate determination and the otherthree bags for determination of dry mass and chemical analyses.The nutrient content, cellulose, lignin, and total phenoliccompounds of manure were determined. In addition, theleachate of manure was collected for nutrient analyses.Samples of the wetland benthic sediment were collected forbenthic invertebrate determination and particle size analyses.Spearman rank correlation was used to evaluate the relationshipbetween chemical compounds of manure and breakdownrate. Principal component analysis was used to explore invertebrateassemblage composition of manure and sediment duringthe experiment.Results and discussion Limnodrilus, Dero, and Chironomuswere dominant in the manure. Gatherer-collector was the dominant group in the manure, comprising almost 95 % ofthe total density of invertebrates. Breakdown rate was significantlyrelated with nutrients, cellulose and total phenolics ofmanure. An ordination plot showed changes in invertebrateassemblages of manure and sediment samples over time.Conclusions This study provides new insight on the importanceof manure as a substrate for macroinvertebrate colonization.Cattle manure needs to be considered as a potentialsource of nutrients for aquatic systems and a substrate forinvertebrate assemblages.","palabras_clave":"BENTHIC FAUNA|CHEMICAL COMPOUNDS|FUNCTIONAL FEEDING GROUPS|LIVESTOCK"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4476,"idioma_id":4,"titulo":"Unravelling the role of determinism and stochasticity in structuring the phytoplanktonic metacommunity of the Paraná River floodplain","resumen":"One of the ongoing debates around metacommunity ecology is to what extent stochastic and deterministic processes act on community assembly. We explored the influence of both determinism, mediated by environmental filters, and stochasticity, mediated by dispersal and ecological drift, on phytoplankton assembly in floodplains of rivers. A probabilistic co-occurrence model revealed the presence of 94.1% random and 5.9% non-random species pairwise associations. The latter were higher at both hydrologically isolated (4.42%) and connected environments (2.2%). Variation partitioning analysis showed similar significant explanations by the unique environmental (7.7%, Secchi, conductivity, vegetation, phosphorous) and spatial (7.2%, watercourse distance, longitude) components. Temporal variability was poorly represented (2.4%) because we only considered two low-water periods. Species co-occurrence patterns showed that most taxa coexist randomly. The environmental explanation is in line with niche-assembly models (species sorting), but the similar proportion explained by spatial organisation related to random dispersal guides the evidence to both deterministic and stochastic processes. The higher percentage of random co-occurrence and the larger assemblage variability observed in isolated environments suggests that random dispersal, ecological drift, and priority effects could promote stochasticity. We concluded that both groups of processes affect the structure of phytoplankton metacommunities in a floodplain system and suggest the preponderance of stochastic organisation.","palabras_clave":"DISPERSAL|SPATIAL STRUCTURE |CO-OCCURRENCE PATTERNS |PROBABILISTIC MODELS |RANDOM ASSOCIATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4477,"idioma_id":4,"titulo":"Smart, Sustainable, and Ecofriendly Chemical Design of Fully Bio-based Thermally Stable Thermosets Based on Benzoxazine Chemistry","resumen":"A smart synthetic chemical design incorporating furfurylamine, a natural renewable amine, into a partially bio-based coumarin-containing benzoxazine is presented. The versatility of the synthetic approach is shown to be flexible and robust enough to be successful under more ecofriendly reaction conditions by replacing toluene with ethanol as the reaction solvent and even under solventless conditions. The chemical structure of this coumarin-furfurylamine-containing benzoxazine is characterized by FTIR, 1H NMR spectroscopy and two-dimensional 1H?1H nuclear Overhauser effect spectroscopy (2D 1H?1H NOESY). The thermal properties of the resin toward polymerization are characterized by differential scanning calorimetry (DSC) and the thermal stability of the resulting polymers by thermogravimetric analysis (TGA). The results reveal that the furanic moiety induces a co-operative activating effect, thus lowering the polymerization temperature and also contributes to a better thermal stability of the resulting polymers. These results, in addition to those of natural renewable benzoxazine resins reviewed herein, highlight the positive and beneficial Implication of designing novel bio-based polybenzoxazine and possibly other thermosets with desirable and competitive properties.","palabras_clave":"N.A."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4478,"idioma_id":4,"titulo":"Feeding selectivity of the invasive mussel Limnoperna fortunei (Dunker, 1857) on a natural phytoplankton assemblage: what really matters?","resumen":"The aims of this work were to analyse the feeding selectivity of L. fortunei in a natural assemblage of phytoplankton in a short-term microcosm experiment and to assess whether this selectivity is affected by the presence of Rotifera as a secondary, palatable feeding resource. This bivalve preferred Desmidiales, Chlorococcales, Euglenophyceae and Chrysophyceae algae with a maximum linear dimension from 20 to 100 lm. Organisms between 500 and 40 9 103 lm3 belonging to Desmidiales, Chrysophyceae and Euglenophyceae were also positively selected. Volvocales, Cryptophyceae and one group of medium-size Euglenophyceae (Trachelomonas sp.) had a high, negative selectivity index independent of their cell shape or size (Ivlev?s index of feeding selectivity -0.7). The mussel positively selected Rotifera, and this only had a measurable effect on large Euglenophyceae, which increase their selectivity value in the absence of Rotifera. The nonparametric multiplicative regression showed that selectivity is largely explained by a combination of cell shape, biovolume and the phytoplankton taxa offered (R2[0.8). We concluded that the impact on phytoplankton community structure could be severe, considering that the presence of zooplankton does not have an effect on the majority of phytoplankton groups and that the mussel tends to feed on both items to improve its diet. The negative selection of some phytoplankton taxa is possibly related to the morphophysiological characteristics of their cell shells.","palabras_clave":"PHYTOPLANKTON|SELECTIVE FEEDING|INVASIVE MUSSEL SPECIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4479,"idioma_id":4,"titulo":"Influence of macrophyte integrity on zooplankton habitat preference, emphasizing the released phenolic compounds and chromophoric dissolved organic matter","resumen":"Macrophyte health status can influence the composition of their exudates causing different effects on zooplankton behavior and distribution in nature. We hypothesize that: (1) the release of phenolic compounds and chromophoric dissolved organic matter (CDOM) depends on macrophyte species and its health status (broken macrophytes: BM, or healthy macrophytes: HM); (2) the repellency effect depends on zooplankton species, macrophyte species and its health status; and (3) higher concentrations of phenolic compounds and CDOM produce a stronger repellency effect. Phenolic compounds and CDOM were analyzed in exudates of BM and HM of Salvinia sp., Eichhornia crassipes, Pistia stratiotes, Azolla sp. and Ludwigia peploides. Through a flow-through experiment, the repellency produced by these exudates was assessed in two copepods (Notodiaptomus conifer and Argyrodiaptomus falcifer) and one cladoceran (Ceriodaphnia dubia). Our hypotheses were partially validated. The quantity of exudated phenolic compounds and CDOM depended on macrophyte species and, to a lesser extent, on the plant health status. The repellency effect was affected by macrophyte and zooplankton species but not by the health status of plants. Only C. dubia and A. falcifer increased their evasion behavior when phenolic compound and CDOM concentrations increased. In brief, the structuring effect of repellent substances depends on different factors. Under a certain threshold concentration, zooplankton behavior might depend on the information associated with the plant odor (e.g., predation risk, structural complexity) more than on the quantity of the released chemical compounds. Above this threshold, evasion would be the only possible option to avoid damaging effects.","palabras_clave":"AQUATIC PLANTS |CHEMICAL ECOLOGY |EVASION BEHAVIOR |MICROCRUSTACEANS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4480,"idioma_id":4,"titulo":"Dynamic speckle image segmentation using Self-Organizing Maps","resumen":"The aim of this work is to build a computational model able to automatically identify, after training, dynamic speckle pattern regions with similar properties. The process is carried out using a set of descriptors applied to the intensity variations with time in every pixel of a speckle image sequence. An image obtained by projecting a self-organized map is converted into regions of similar activity that can be easily distinguished. We propose a general rocedure that could be pplied to numerous situations. As examples we show different situations: (a) an activity test in a simplified situation; (b) a non-biological example and (c) biological active specimens. The results obtained are ncouraging; they significantly improve upon those obtained using a single descriptor and will eventually permit automatic quantitative assessment.","palabras_clave":"dynamic speckle|optical processing|self-organizing maps|computational intelligence|SOM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4481,"idioma_id":4,"titulo":"Phosphatidylinositol 3-Kinase\/AKT Pathway Inhibition by Doxazosin Promotes Glioblastoma Cells Death, Upregulation of p53 and Triggers Low Neurotoxicity","resumen":"Glioblastoma is the most frequent and malignant brain tumor. Treatment includes chemotherapy with temozolomide concomitant with surgical resection and\/or irradiation. However, a number of cases are resistant to temozolomide, as well as the human glioblastoma cell line U138-MG. We investigated doxazosin's (an antihypertensive drug) activity against glioblastoma cells (C6 and U138-MG) and its neurotoxicity on primary astrocytes and organoptypic hippocampal cultures. For this study, the following methods were used: citotoxicity assays, flow cytometry, western-blotting and confocal microscopy. We showed that doxazosin induces cell death on C6 and U138-MG cells. We observed that doxazosin's effects on the PI3K\/Akt pathway were similar as LY294002 (PI3K specific inhibitor). In glioblastoma cells treated with doxasozin, Akt levels were greatly reduced. Upon examination of activities of proteins downstream of Akt we observed upregulation of GSK-3&#946; and p53. This led to cell proliferation inhibition, cell death induction via caspase-3 activation and cell cycle arrest at G0\/G1 phase in glioblastoma cells. We used in this study Lapatinib, a tyrosine kinase inhibitor, as a comparison with doxazosin because they present similar chemical structure. We also tested the neurocitotoxicity of doxazosin in primary astrocytes and organotypic cultures and observed that doxazosin induced cell death on a small percentage of non-tumor cells. Aggressiveness of glioblastoma tumors and dismal prognosis require development of new treatment agents. This includes less toxic drugs, more selective towards tumor cells, causing less damage to the patient. Therefore, our results confirm the potential of doxazosin as an attractive therapeutic antiglioma agent.","palabras_clave":"PI3\/AKT|Doxasonin|Glioblastoma"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4482,"idioma_id":4,"titulo":"Isolation and Characterization of Ischemia-Derived Astrocytes (IDAs) with Ability to Transactivate Quiescent Astrocytes","resumen":"Reactive gliosis involving activation and proliferation of astrocytes and microglia, is a widespread but largely complex and graded glial response to brain injury. Astroglial population has a previously underestimated high heterogeneity with cells differing in their morphology, gene expression profile, and response to injury. Here, we identified a subset of reactive astrocytes isolated from brain focal ischemic lesions that show several atypical characteristics. Ischemia-derived astrocytes (IDAs) were isolated from early ischemic penumbra and core. IDA did not originate from myeloid precursors, butrather from pre-existing local progenitors. Isolated IDA markedly differ from primary astrocytes, as they proliferate in vitro with high cell division rate, show increased migratory ability, have reduced replicative senescence and grow in the presence of macrophages within the limits imposed by the glial scar. Remarkably, IDA produce a conditioned medium that strongly induced activation on quiescent primary astrocytes and potentiated the neuronal death triggered by oxygen-glucose deprivation. When re-implanted into normal rat brains, eGFP-IDA migrated around the injection site and induced focal reactive gliosis. Inhibition of gamma secretases or culture on quiescent primary astrocytes monolayers facilitated IDA differentiation to astrocytes. We propose that IDA represent an undifferentiated, pro-inflammatory, highly replicative and migratory astroglial subtype emerging from the ischemic microenvironment that may contribute to the expansion of reactive gliosis.","palabras_clave":"REACTIVE GLIOSIS|INFLAMMATION|ISCHEMIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4483,"idioma_id":4,"titulo":"UV-Visible Spectroscopy and Multivariate Classification as a Screening Tool for Determining the Adulteration of Sauces","resumen":"A rapid, simple, and economic multivariate screening methodology based on UV-visible spectroscopy and multivariate classification is proposed to test for adulteration in sauces. Two classification strategies were evaluated to compare their ability to detect food fraud: untargeted modeling (class modeling) and targeted classification (discriminant analysis). As a case study, the possible adulteration of ketchups and barbecue sauces with the banned Sudan I dye was considered. The classification models were built with a new classification tool for class modeling (partial least squares-density modeling, PLS-DM) and with the classical discriminant partial least squares-discriminant analysis (PLS-DA). Very satisfactory classification results were obtained with both strategies: regarding untargeted modeling, only original samples (class 1) were modeled obtaining a 94.5 % of correct classification and regarding targeted classification, two classes were considered (class 1 original samples and class 2 adulterated samples) with an overall classification rate of 97.3 %. The two strategies are useful and adequate as screening tools for monitoring the quality of sauces especially in situations that require quick responses.","palabras_clave":"UV-VISIBLE SPECTROSCOPY|MULTIVARIATE CLASSIFICATION|ADULTERATION|SAUCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4484,"idioma_id":4,"titulo":"Fine-scale spatial genetic structure analysis in two Argentine  populations of Prosopis alba (Mimosoideae) with different levels of ecological disturbance","resumen":"Spatial genetic structure (SGS) in plants is primarily determined by the interaction between pollen and seed dispersal, but it is strongly affected by both evolutionary and ecological processes. SGS studies in forest species also allow evaluating the consequences of human-mediated disturbance on pollen and seed movement and designing strategies of sustainable use of native forest resources. The present paper compares fine-scale SGS between two populations of Prosopis alba: Fernandez-Forres (FF) and Campo Duran (CD), based on the variation of 12 SSR markers. Populations show different history, management, and levels of disturbance. FF is highly disturbed and fragmented by agricultural activity and stock-breeding. Although no significant differences were observed in genetic variability parameters, highly significant genetic differentiation was detected with virtually no admixture between populations. SGS was positive and significant at short distances only in the non-disturbed population of CD. Accordingly, estimated neighborhood size and effective gene dispersal are higher in FF than in CD. This result might be explained by the higher incidence of livestock in seed dispersal and the patchy structure favoring longer pollen movement and artificial thinning and selection in FF. The results are relevant to conservation and breeding programs, suggesting that the distance between seed trees to be sampled should be larger than 22 m, the estimated distance of significant SGS in P. alba.","palabras_clave":"AUTOCORRELATION ANALYSIS|ISOLATION BY DISTANCE| NEIGHBORHOOD SIZE|POLLEN DISPERSAL|SPATIAL GENETIC STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4485,"idioma_id":4,"titulo":"Influence of chitosan coating on magnetic nanoparticles in endothelial cells and acute tissue biodistribution","resumen":"Chitosan coating on magnetic nanoparticles (MNPs) was studied on biological systems as a first step toward the application in the biomedical field as drug-targeted nanosystems. Composition of MNPs consists of magnetite functionalized with oleic acid and coated with the biopolymer chitosan or glutaraldehyde-cross-linked chitosan. The influence of the biopolymeric coating has been evaluated by in vitro and in vivo assays on the effects of these MNPs on rat aortic endothelial cells (ECs) viability and on the random tissue distribution in mice. Results were correlated with the physicochemical properties of the nanoparticles. Nitric oxide (NO) production by ECs was determined, considering that endothelial NO represents one of the major markers of ECs function. Cell viability was studied by MTT assay. Different doses of the MNPs (1, 10 and 100&#8201;&#956;g\/mL) were assayed, revealing that MNPs coated with non-cross-linked chitosan for 6 and 24 h did not affect neither NO production nor cell viability. However, a significant decrease in cell viability was observed after 36 h treatment with the highest dose of this nanocarrier. It was also revealed that the presence and dose of glutaraldehyde in the MNPs structureimpact on the cytotoxicity. The study of the acute tissue distribution was performed acutely in mice after 24 h of an intraperitoneal injection of the MNPs and sub acutely, after 28 days of weekly administration. Both formulations greatly avoided the initial clearance by the reticuloendothelial system (RES) in liver. Biological properties found for N1 and N2 in the performed assays reveal that chitosan coating improves biocompatibility of MNPs turning these magnetic nanosystems as promising devices for targeted drug delivery.","palabras_clave":"MAGNETIC CARRIERS|NANOPARTICLES|CHITOSAN|ENDOTHELIAL CELLS|BIODISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4486,"idioma_id":4,"titulo":"Chitin hydrogel reinforced with  TiO2  nanoparticles  as an Arsenic sorbent","resumen":"In this work chitin hydrogels were reinforced with TiO2 nanoparticles acting as fillers. The mechanical and physicochemical characteristics of these hydrogels were studied by oscillatory rheology, infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Small Angle X-ray Spectroscopy (SAXS). Adsorption showed to be higher at pHs below the TiO2 point of zero charge (pH&asymp;6.9). The equilibrium was achieved within 4h following an Elovich kinetic model. The equilibrium assay showed a maximum capacity of 3.1mg\/g with better fitting for the Langmuir model. Moreover, the homogeneity\/heterogeneity parameters of the Sips, Toth and Redlich-Peterson models were close to a value of 1, indicating a homogeneous sorbent\/sorbate interaction. Typical natural water ions competed with As(V) for the sorption sites, being sulfate the most relevant interference. This interference was also evidenced in As(V) adsorption from natural water samples where the sorption capacity varied depending the anion composition of the sample. The reutilization of the hybrid gel was assessed up to five adsorption cycles.","palabras_clave":"CHITIN|TITANIUM DIOXIDE|ARSENIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4487,"idioma_id":4,"titulo":"Antimicrobial surface functionalization of PVC by a guanidine based antimicrobial polymer","resumen":"Antimicrobial polyvinyl chloride (PVC) plastic was obtained by covalent bonding of poly hexamethylenediamine guanidine hydrochloride (PHMG), a guanidine based antimicrobial polymer. This was achieved by grafting mercaptopropyltrimethoxysilane onto PVC, followed by aminopropyltriethoxysilane. Glutaraldehyde is a bifunctional crosslinker that was bonded to the free amine groups found in the treated PVC on one side and PHMG on the other. The treated PVC samples were characterized by FT-IR and XPS, showing that the PVC samples were successfully modified. Energy Dispersive X-ray spectroscopy showed the spatial distribution of the elements Si and S, indicating that the coatings were homogeneous. The resulting PVC samples showed high antimicrobial activityagainst Gram-positive and Gram-negative bacteria. Furthermore, the biofilm formation was negligible in comparison with the untreated material. The coating elusion assay indicated that its antimicrobial ability was achieved via direct contact rather than a controlled release mechanism.","palabras_clave":"ANTIMICROBIAL COATING|PVC|PHMG|NON-LEACHING COATING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4488,"idioma_id":4,"titulo":"Influence of the addition of Amaranthus mantegazzianus flour on the nutritional and health properties of pasta","resumen":"To improve the nutritional and functional properties of pasta, bread wheat flour was substituted with wholemeal flour from Amaranthus mantegazzianus (WMAF) at 15, 30, 40 and 50% w\/w. Proteins, dietary fibre, glycemic index (GI) and carbohydrate and protein digestibility were determined in the resulting pasta. Squalene content was also measured, in view of the beneficial health properties of this compound.With the addition of 30% of WMAF, where the technological quality of pasta remained acceptable, protein and fibre contents resulted in 23 and 50% higher than control pasta. Pasta presented a significant enrichment of squalene content at all levels of substitution with WMAF. In addition, all samples remained in the range of low GI foods, and protein digestibility values were even higher than control pasta","palabras_clave":"amaranth flour;|Carbohydrate digestibility|Squalene|pasta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4489,"idioma_id":4,"titulo":"Ultraviolet-B radiation, water deficit and abscisic acid: a review of independent and interactive effects on grapevines","resumen":"Viticulture is present around the word in a large diversity of climates. The water availability in the soil and the quality of sunlight are two environmental factors that affect the fruit characteristics for winemaking. Ultraviolet (UV)-B radiation comprises a small fraction of sunlight that reaches the Earth?s surface, but has enough energy to cause large photobiological effects on higher plants. High UV-B increases metabolites with antioxidant properties as phenolic and volatile organic compounds in berries that improve oenological quality although affecting growth and fruit yield. Water restriction is a common cultural practice used in many wine regions to increase berry quality for winemaking and it is well documented that main effects are mediated by abscisic acid (ABA). Generally, ABA is a phytohormone that, besides to control stomatal aperture, regulates many physiological and biochemical processes of acclimation to adverse environmental conditions; and also controls grape berry maturation. Stress conditions and\/or environmental signals generally increase ABA, and a promotive effect by UV-B has been found in grapevines. This review provides an overview of existing literature on the effects of UV-B radiation, moderate water deficit practices and sprayed ABA on grapevines (Vitis vinifera L.). The focus is on the physiological and biochemical aspects affecting growth, yield and quality for winemaking.","palabras_clave":"ABA|DROUGHT|SECONDARY METABOLISM|UV-B|VITIS VINIFERA L."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4490,"idioma_id":4,"titulo":"Palo Blanco: a village in the Late and Inka landscapes in the Hualfín Valley (Catamarca, Argentina)","resumen":"In this paper we analyze archaeological data from Palo Blanco, located in the center of the Hualfín Valley (Dept. of Belén, Catamarca, Argentina) to discuss how a relational concept of landscape provides significant tools to reconstruct links between a local village and different landscapes configured during Late and Inka times (ca. 1400-1500 AD). Also, we present how this approach allows us to develop an alternative scheme to change interpretations of the past in the region. Based on the analysis of spatial, social and temporal dimensions, we analyze archaeological evidence focusing on placement, building, flow of bodies, spatial perception, relationships between people and material culture, and chronology. Finally, we consider Palo Blanco as a typical local defensive village during late pre-Inka times, that probably changed its role during Inka conquest.","palabras_clave":"Valle de Hualfín|Palo Blanco |Período Tardío|Paisaje"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4491,"idioma_id":4,"titulo":"Metal-Organic Frameworks Help Conducting Polymers Optimize the Efficiency of the Oxygen Reduction Reaction in Neutral Solutions","resumen":"The anchoring of an electro-inactive metal-organic framework (ZIF-8 MOF) layer on a conducting polymer-modified electrode enhances its electrocatalytic efficiency toward the ORR in neutral solutions by a selective uptake and preconcentrationof oxygen in the ZIF-8 microporous matrix that ultimately acts as an oxygen reservoir in intimate contact with the electroactive material.","palabras_clave":"METAL ORGANIC FRAMEWORKS|CONDUCTING POLYMERS|OXYGEN REDUCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4492,"idioma_id":4,"titulo":"Recent Developments in the Layer-by-Layer Assembly of Polyaniline and Carbon Nanomaterials for Energy Storage and Sensing Applications. From Synthetic Aspects to Structural and Functional Characterization","resumen":"The construction of hybrid polymer?inorganic nanoarchitectures for electrochemical purposes based on the layer-by-layer assembly of conducting polymers and carbon nanomaterials has become increasingly popular over the last decade. This explosion of interest is primarily related to the increasing mastery in the design of supramolecular constructs using simple wet chemical approaches. Concomitantly, this continuous research activity paved the way to the rapid development of nanocomposites or 'nanoblends' readily integrable into energy storage and sensing devices. In this sense, the layer-by-layer (LbL) assembly technique has allowed us to access three-dimensional (3D) multicomponent carbon-based network nanoarchitectures displaying addressable electrical, electrochemical and transport properties in which conducting polymers, such as polyaniline, and carbon nanomaterials, such as carbon nanotubes or nanographene, play their unique roles without disrupting their inherent functions ? complementary entities coexisting in harmony. Over the last few years the level of functional sophistication reached by LbL-assembled carbon-based 3D network nanoarchitectures, and the level of knowledge related to how to design, fabricate and optimize the properties of these 3D nanoconstructs have advanced enormously. This feature article presents and discusses not only the recent advances but also the emerging challenges in complex hybrid nanoarchitectures that result from the layer-by-layer assembly of polyaniline, a quintessential conducting polymer, and diverse carbon nanomaterials. This is a rapidly developing research area, and this work attempts to provide an overview of the diverse 3D network nanoarchitectures prepared up to now. The importance of materials processing and LbL integration is explored within each section and while the overall emphasis is on energy storage and sensing applications, the most widely-used synthetic strategies and characterization methods for ?nanoblend? formation and performance evaluation are also presented.","palabras_clave":"Layer-by-Layer|Polyaniline|Carbon Nanomaterials"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4493,"idioma_id":4,"titulo":"Constrained Latent Variable Model Predictive Control for trajectory tracking and economic optimization in batch processes","resumen":"A constrained latent variable model predictivecontrol (LV-MPC) technique is proposed for trajectory tracking and economicoptimization in batch processes. The controller allows the incorporation ofconstraints on the process variables and is designed on the basis of multi-wayprincipal component analysis (MPCA) of a batch data array rearranged by meansof a regularized batch-wise unfolding. The main advantages ofLV-MPC over other MPC techniquesare: (i) requirements for the dataset are rather modest(only around 10-20 batch runs are necessary), (ii) nonlinearprocesses can efficiently be handled algebraicallythrough MPCA models, and (iii) the tuning procedure is simple. The LV-MPC for trackingis tested through a benchmark process used in previous LV-MPC formulations. Theextension to economic LV-MPC includes an economic cost and it is based on modeland trajectory updating from batch to batch to drive the process to theeconomic optimal region. A data-driven model validity indicator is used toensure the prediction?s validity while the economic cost drives the process toregions with higher profit. This technique is validated through simulations ina case study.","palabras_clave":"MULTI-WAY PRINCIPAL COMPONENT ANALYSIS|LATENT VARIABLE MODEL|CONSTRAINED PREDICTIVE CONTROL|ECONOMIC COST FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4494,"idioma_id":4,"titulo":"Amine-appended polyaniline as a water dispersible electroactive polyelectrolyte and its integration into functional self-assembled multilayers","resumen":"We present an effective and simple method for the synthesis of poly(3-aminobenzylamine) (PABA) using a chemical oxidation strategy in aqueous solution. The polymer was characterized by NMR, LDI-TOF mass spectrometry, UV-visible, XPS and ATR-FTIR spectroscopies. Stable acidic dispersions were employed for the construction of layer-by-layer assembled films with polyanions, which effectively act as dopants of the electroactive component as revealed by spectroscopic analysis. The assembled films were electroactive in neutral solutions, probably owing to the combination of the doping effect by the polyanions and the self-doping effect of the protonated amino groups of the PABA backbone. These layers showed an electrocatalytic effect on the ascorbic acid oxidation. The advantages of employing PABA instead of polyaniline include improved electroactivity in neutral solution, good processability owing to its higher solubility in acidic solutions and increased interaction with anionic counterparts, which may propel its integration with electroactive biomolecules or conducting nanomaterials for the design of bioelectrochemical devices and energy storage applications.","palabras_clave":"POLYAMINOBENZYLAMINE (PABA)|POLYELECTROLYTE MULTILAYERS|LAYER-BY-LAYER (LBL) ASSEMBLIES|ELECTROACTIVE POLYMERS|POLYANILINE (PANI)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4495,"idioma_id":4,"titulo":"Pulmonary Vascular Morphology as an Imaging Biomarker in Chronic Thromboembolic Pulmonary Hypertension","resumen":"Pulmonary Vascular Morphology as an Imaging Biomarker in Chronic Thromboembolic Pulmonary Hypertension.","palabras_clave":"chronic thromboembolic pulmonary hypertension"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4496,"idioma_id":4,"titulo":"First study of fossil rodent middens as source of paleoparasitological evidences (northwestern Patagonia, Argentina)","resumen":"The present paper reports the first paleoparasitological results obtained fromcoprolites of fossil rodent middensand demonstrates the potential of rodent middens as a source of paleoparasitological evidences in SouthAmerica. Ten fossil rodent middens from northwestern Patagonia, Argentina, were studied. Five coprolites ofeach midden were fully processed, rehydrated, homogenized, subjected to spontaneous sedimentation, and examinedthrough light microscopy. Eight of the 10 examined rodent middens contained parasite eggs. The eggs ofparasites were assigned to Heteroxynema (Cavioxyura) viscaciae Sutton &amp; Hugot, 1989 and Helminthoxys sp.(Nematoda: Oxyuridae), Trichuris sp. (Nematoda: Trichuridae) and one unidentified nematode. Fossil rodentmiddens were assigned to Lagidium viscacia (Caviomorph: Chinchillidae). The excellent preservation of parasiteremains in coprolites from fossil rodent middens provided an opportunity to perform paleoparasitological inferences.The results of this papers demonstrates that fossil rodent middens offer an excellent opportunity for therecovery of parasite remains for future paleoparasitological studies in the southwest of South America.","palabras_clave":"PALEOPARASITOLOGIA|FOSSIL RODENT MIDDENS|COPROLITES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4497,"idioma_id":4,"titulo":"Presence, abundance and bacterivory of the mixotrophic algae Pseudopedinella (Dictyochophyceae) in freshwater environments","resumen":"The genus Pseudopedinella has been described as mixotrophic; however, ecological information about this algal stramenopile (Heterokonta) is unclear. We investigate the environmental conditions that determine the presence, abundance and bacterivory rates of this genus in freshwater systems. To this end we analyzed 54 water bodies with different limnological features distributed along a latitudinal and trophic gradient in northern Patagonia (Argentina) and the Antarctic Peninsula. In addition, 14 grazing experiments were carried out in order to estimate ingestion rates and impact on the bacterioplankton. Our results indicate that this genus is exclusively found in oligotrophic environments, and that it develops well in a wide range of temperatures. Average cell-specific grazing rate was 2.83 bacteria cell?1 h?1, with a maximum value of 6.74 bacteria ml?1 h?1. Interestingly, grazing increased with prey abundance and decreased with increasing nutrient availability. These patterns are common in highly bacterivorous protists that use phagotrophy as a main source of nutrient acquisition. Despite their usually low abundance (avg. 182 cells ml?1), this single genus was responsible for up to 24% (avg. 10%) of the total grazing impact exerted by all phagotrophs in these lakes. Overall, our results support the idea that Pseudopedinella is a highly bacterivorous group of freshwater protists, with the ability to develop well in oligotrophic conditions and with a potentially significant impact on bacterioplankton.","palabras_clave":"PSEUDOPEDINELLA|BACTERIVORY|GRAZING RATE|PATAGONIAN LAKES|ANTARCTIC LAKES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4499,"idioma_id":4,"titulo":"Synthesis and characterization of a novel tungstosilicic acid immobilized on zeolites catalyst for the photodegradation of methyl orange","resumen":"Materials based on tungstosilicic acid (TSA) immobilized on zeolites (NH4Y and NH4ZSM5) were preparedby wet impregnation of the zeolite matrix with TSA aqueous solutions. The concentration was varied inorder to obtain TSA contents of 5%, 10%, 20%, and 30% w\/w in the solid. Catalysts were further character-ized by N2adsorption?desorption isotherms, FT-IR, XRD, DRS-UV?vis and SEM techniques. The resultingmaterials were evaluated in the photodecomposition of azo dye methyl orange. The results revealedthat the materials present suitable properties to be used as catalysts in the photocatalytic treatment ofwastewater containing dyes, and the photodegradation follows a pseudo-first-order kinetics. Reactionparameters such as TSA content, catalysts mass, reuses and pH, were evaluated.","palabras_clave":"Photocatalysis|Zeolites|Tungstosilicic acid|Azoic dye"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4500,"idioma_id":4,"titulo":"Dodecatungstocobaltate supported over ZSM-5 zeolite as novel solid catalyst in selective sulfide oxidation","resumen":"The catalytic behavior of a Keggin type potassium dodecatungstocobaltate (II) salt supported on ZSM-5 zeolite for wet peroxide oxidation of 2-(methylthio)benzothiazole, as a model organic sulfide, was thoroughly studied. Microporous ZSM-5 zeolite was obtained by the hydrothermal crystallization method. The Keggin salt was incorporated in the MFI zeolite matrix by the wet impregnation method followed by calcination at350 ºC. Catalysts were further characterized by X Ray Diffraction, UV-vis Diffuse Reflectance spectroscopy and Scanning Electron Microscopy - EDAX techniques. Supported and bulk Keggin dodecatungstocobaltate (II) were compared with pure HZSM- 5 zeolite as catalysts for the sulfide oxidation. When the reaction was not catalyzed, just a 9 mol% of sulfide conversion was obtained. Reaction parameters, such as nature of the solvent, hydrogen peroxide concentration, reaction time, catalyst mass,substrate initial concentration and reaction temperature, were evaluated to reach the optimum reaction conditions, considering substrate  conversion and sulfoxide and sulfone selectivities. Catalyst stability in several oxidation cycles was also examined.","palabras_clave":"Keggin heteropolycompound|ZSM-5|2-(methylthio)benzothiazole|mild oxidation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4501,"idioma_id":4,"titulo":"CATIONIC AND ANIONIC POLYELECTROLYTE COMPLEXES OF XYLAN AND CHITOSAN. INTERACTION WITH LIGNOCELLULOSIC SURFACES","resumen":"Cationic (CatPECs) and anionic (AnPECs) polyelectrolyte complexes from xylan and chitosan were formed,characterized and adsorbed onto unbleached fibers for improving the papermaking properties. They wereprepared at a level of 30% of neutralization charge ratio by modifying the order of addition of polyelec-trolytes and the ionic strength (0.01 N and 0.1 N NaCl). The charge density, colloidal stability and particlesize of polyelectrolyte complexes (PECs) was measured using polyelectrolyte titration method, Turbis-can and Zetasizer Nano equipments, respectively. All the complexes were stable even after seven daysfrom PEC formation. DRIFT spectra of complexes were also analyzed. The adsorption behavior of themonto cellulose nanofibrils model surfaces was studied using quartz crystal microbalance with dissipationmonitoring, and surface plasmon resonance. It was found that the PEC layers were viscoelastic and highlyhydrated. Finally, it is shown that the adsorbed PECs onto cellulosic fibers markedly improved the tensileand crushing strengths of paper","palabras_clave":"Complex sizes|Order of addition|Ionic strength|Papermaking properties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4502,"idioma_id":4,"titulo":"Human Polymorphisms in Placentally Expressed Genes and Their Association With Susceptibility to Congenital Trypanosoma cruzi Infection","resumen":"Background. It is currently unclear why only a proportion of children born to Trypanosoma cruzi-infected mothers acquire the infection. We have examined the association of 11 single-nucleotide polymorphisms (SNPs) located in genes coding for placental expression enzymes as genetic markers of susceptibility to congenital T. Cruzi infection (hereafter, 'congenital infection'): rs2014683 and rs1048988 in ALPP; rs11244787 and rs1871054 in ADAM12; rs243866, rs243865, rs17859821, rs243864, and rs2285053 in MMP2; and rs3918242 and rs2234681 in MMP9. Methods. Two groups of children born to mothers seropositive for T. Cruzi were compared: 101 had congenital infection, and 116 were uninfected. Novel high-resolution melting and capillary electrophoresis genotyping techniques were designed and used. Results. Logistic regression analysis showed that mutations in rs11244787 and rs1871054 (in ADAM12) and rs243866, rs17859821, and rs2285053 (in MMP2) were associated with susceptibility to congenital infection. Multifactor dimensionality reduction revealed that genotyping results for rs11244787, rs1871054, rs243866, rs17859821 and rs243864 sites would be a good predictor of congenital infection. Conclusions. Our results suggest an important role of human polymorphisms in proteins involved in extracellular matrix remodeling and the immune response during congenital infection. To our knowledge, this is the first study demonstrating the association between mutations in placentally expressed genes and susceptibility to congenital infection.","palabras_clave":"Congenital Chagas|Polymorphims|SNP|Metalloproteinases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4503,"idioma_id":4,"titulo":"Serological based monitoring of a cohort of patients with chronic chagas disease treated with benznidazole in a highly endemic area of Northern Argentina","resumen":"This study aimed to evaluate well-documented diagnostic antigens, named B13, 1F8 and JL7 recombinant proteins, as potential markers of seroconversion in treated chagasic patients. Prospective study, involving 203 patients treated with benznidazole, was conducted from endemic areas of northern Argentina. Follow-up was possible in 107 out of them and blood samples were taken for serology and PCR assays before and 2, 3, 6, 12, 24 and 36 months after treatment initiation. Reactivity against Trypanosoma cruzi lysate and recombinant antigens was measured by ELISA. The rate of decrease of antibody titers showed nonlinear kinetics with an abrupt drop within the first three months after initiation of treatment for all studied antigens, followed by a plateau displaying a low decay until the end of follow-up. At this point, anti-B13, anti-1F8 and anti-JL7 titers were relatively close to the cut-off line, while anti-T. cruzi antibodies still remained positive. At baseline, 60.8% (45\/74) of analysed patients tested positive for parasite DNA by PCR and during the follow-up period in 34 out of 45 positive samples (75.5%) could not be detected T. cruzi DNA. Our results suggest that these antigens might be useful as early markers for monitoring antiparasitic treatment in chronic Chagas disease.","palabras_clave":"CHAGAS DISEASE|SEROLOGY|TRYPANOSOMA CRUZY|TREATMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4504,"idioma_id":4,"titulo":"Extreme roll angles in Argentine sea bass. Could refuge ease posture and buoyancy control of marine coastal fishes?","resumen":"The swim bladder provides a mechanism for buoyancyregulation in teleosts. However, in certain species, its location can result inan unstable body position, with associated energetic costs assumed formaintaining posture in addition to the energetic demands from swimbladdervolume regulation. Direct observations show that some body-compressed, cave-refugingteleosts that nominally operate near neutral buoyancy may adopt unusual bodyattitudes within crevices. We hypothesize that these fishes may relax theirbuoyancy and posture control mechanisms during periods of rest. A predictionderived from this is that resting fish may adopt a wide range of roll angles(i.e., rotation about their longitudinal axis) inside caves. To quantify thisbehaviour and for testing this hypothesis, tri-axial accelerometers weredeployed on free-living, cave-refuging Argentine sea bass Acanthistius patachonicus, and the relationship between roll angleand a proxy for activity (defined as the vectorial dynamic body acceleration, VeDBA),was analyzed. The results were compared with data available for three other speciesof fishes with disparate body forms and life styles: the pelagic whale shark Rhincodon typus, the dorso-ventrallycompressed benthic great sculpin Myoxocephaluspolyacanthocephalus, and the fusiform and demersal Atlantic cod Gadus morhua. Inactive Argentine seabass adopted a wide variety of roll angles, including extreme ones exceeding 80&#730;,but had lower roll angles closer to an upright posture primarily associatedwith higher activity levels. In contrast, the great sculpin and Atlantic codboth rested at a close to upright roll angle but had higher activity levelsassociated with larger roll angles. Whale shark did not rest for the durationof the recorded period and also showed higher activity levels associated withlarger roll angles. We propose that relaxation of buoyancy and posture control mayhelp to reduce the metabolic rate in laterally-compressed, cave-refuging fishesduring periods of rest within crevices.","palabras_clave":"POSTURE AND BOUAYANCY COST|MARINE FISH|SWIM BLADDER|SERRANIDAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4505,"idioma_id":4,"titulo":"Cold birds under pressure: Can thermal substitution ease heat loss in diving penguins?","resumen":"Thermoregulation could represent a significant fraction of the total energy budget ofendotherms under unfavorable environmental conditions. This cost affects severaltraits of the ecology of an organism such as its behaviour, distribution, or life history.Heat produced by muscle contraction during activity can be used to pay for heat loss orthermoregulation in many species (known as 'thermal substitution'). This study seeksto unite the effects of temperature, depth, and activity on the energetic costs ofendotherm divers using the Magellanic Penguin as model species and to evaluate ifpenguins may benefit from thermal substitution. This species operates under highlyvariable temperature and depth conditions along its breeding range and provides anideal natural experiment. A developed thermodynamic model describing foragingactivity predicted that the major element affecting heat loss was depth, exacerbated bytemperature. Birds living in colder waters are predicted to be able to minimize costs byexecuting shallower dives and benefit from thermal substitution by swimming faster,particularly during deeper dives. The model was evaluated in two contrastingscenarios: (i) when birds swim near the surface commuting to the foraging areas and(ii) when birds dive to depth to forage. Activity data from tags on free-living penguinsindicated two of these predictions were apparent; penguins generally travelled fasterwhile commuting at the surface in colder waters while birds from warmer watercolonies dived deeper while foraging. Contrary to predictions, however, penguinsswam slower at deeper depths during both descent and ascent phases of foragingdives. These results suggest that penguins may benefit from thermal substitution byswimming faster when birds perform shallow dives commuting to and back fromforaging areas but they provide no evidence of behavioral response (via swimmingfaster) for thermoregulation when diving to depth to forage. Reasons for this arediscussed and include the relevance of prey abundance in 3-d space and maximizing dive duration by conserving oxygen reserves. The way the bird operates will haveprofound consequences for the energy needed and therefore necessary energyacquisition rates. Expansion of our findings to other diving endotherms might helpexplain both global activity patterns and energy flow in ecosystems","palabras_clave":"THERMAL SUBSTITUTION|METABOLIC HEAT PRODUCTION|HEAT LOSS|DIVING BEHAVIOUR|MAGELLANIC PENGUIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4506,"idioma_id":4,"titulo":"Assessment of the Quality of Chronic Anticoagulation Control With Time in Therapeutic Range in Atrial Fibrillation Patients Treated With Vitamin K Antagonists by Hemostasis Specialists: The TERRA Registry: Tiempo en rango en la Republica Argentina","resumen":"Background: Oral anticoagulation therapy with vitamin K antagonists (VKA) such as warfarin and acenocoumarol is recommended in patients with atrial fibrillation (AF) and risk factors for embolism. The quality of anticoagulation control with VKA may be assessed by the time in therapeutic range (TTR). In our country, there are no data available about the quality of anticoagulation in patients with AF. The primary goal of our study was to assess the level of effective anticoagulation in a multicenter network of anticoagulation clinics in Argentina, which included patients with nonvalvular AF (NVAF) treated with VKA oral anticoagulants. Methods: The TERRA trial is a multicenter, cross-sectional study involving 14 anticoagulation clinics that were invited to participate and recruit 100 consecutive patients with NVAF treated with VKA for more than 1 year. The international normalized ratio (INR) values were retrospectively obtained from patient charts, and TTR was calculated using the Rosendaal method. Results: A total of 1190 patients were included in the analysis. Mean age was 74.9 SD9.9 years, and 52.5% of the patients were male. Median TTR was 67.5% (interquartile interval 54-80). During 55% of the TTR, INR was higher than 3. Interinstitution variability was substantial, with a range of 57.7% SD17% to 87.7% SD17%, P lower than 0.001. The 10th percentile of TTR was 41%, the 20th percentile was 50%, the 30th was 58%, and the 35th percentile was 60%. In 40% of patients, TTR was lower than 70%. Conclusion: In this multicenter study, mean TTR values in patients with AF under VKA were similar to those in international therapeutic clinical trials (55%-65%). Marked variations among institutions were observed and, although average results obtained were high, one third of the patients exhibited a TTR below 60%. This cutoff value is conservative according to current recommendations, and guidelines suggest that when management with VKA cannot be improved, patients should be switched to direct oral anticoagulants. The addition of TTR calculation to clinical practice may help improve the quality of oral anticoagulation in patients with AF, thus improving anticoagulation outcomes.","palabras_clave":"ANTICOAGULANTS|ATRIAL FIBRILLATION|TIME IN THERAPEUTIC RANGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4507,"idioma_id":4,"titulo":"Length-weight relationships for two abundant rocky reef fishes from Northern Patagonia, Argentina: Sebastes oculatus Valenciennes, 1833 and Pinguipes brasilianus Cuvier, 1829","resumen":"The Patagonian rockfish Sebastes oculatus and the Brazilian sandperch Pinguipesbrasilianus are two of the most abundant, conspicuous reef fishes in the northern Patagonian gulfs of Argentina. Here, we provide separate length-weight relationships for males, females and pooled individuals of these species for the North Patagonian region, based on samples collected between 2009 and 2014, throughout the year, between 42°S and 45°S. No length-weight relationships for P. brasilianus are available in the literature. Besides, although one relationship was published for S. oculatus from Chilean waters, the limited number of fish and size range did not allow estimating the model parameters accurately.","palabras_clave":"sebastes oculatus|pinguipes brasilianus|Length-weight relationships|patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4508,"idioma_id":5,"titulo":"Retrocesos desde el Sur Las reformas a la Ley de Servicios de Comunicación Audiovisual en Argentina. Del paradigma de derechos humanos al modelo de negocios.","resumen":"El 22 de noviembre de 2015, el ingeniero Mauricio Macri se impuso en la segunda vuelta electoral de las elecciones presidenciales en Argentina. A partir del día de su asunción, el 10 de diciembre, el Congreso de la Nación entró formalmente en receso hasta el 1° de marzo de 2016. Durante ese período, el Poder Ejecutivo declinó la facultad de convocar a sesiones extraordinarias para tratar sus propios proyectos de ley y avanzó, desde las primeras horas de su mandato, con la firma de numerosos decretos presidenciales.En apenas 5 días hábiles Macri firmó más de 100 decretos. Algunos de ellos referidos a cuestiones cuya regulación por esa vía se encuentra expresamente prohibida por la Constitución Nacional, como ocurre con la legislación vinculada a la defensa y promoción del patrimonio audiovisual nacional, facultad exclusiva del Parlamento. El avance a través de decretos también contradice los estándares fijados por el Sistema Interamericano de Derechos Humanos en la materia. La Relatoría Especial para la Libertad de Expresión de la OEA en su Informe del año 2009 sostuvo que ?la regulación sobre radiodifusión, en tanto puede implicar una limitación al ejercicio del derecho a la libertad de expresión, debe estar establecida en forma previa y de manera expresa, taxativa, precisa y clara en una ley, tanto en el sentido formal como material?. Al respecto, es aplicable la definición de la Corte Interamericana en la Opinión Consultiva OC-6\/86, según la cual, la expresión ?leyes? no significa cualquier norma jurídica, sino ?aquellos actos normativos generales adoptados por el órgano legislativo constitucionalmente previsto y democráticamente elegido, según los procedimientos establecidos en la Constitución, ceñidos al bien común?. Para poder dimensionar las transformaciones dispuestas resulta útil evaluar desde una perspectiva de derechos humanos, aunque de un modo breve, la magnitud de los cambios en comparación con las leyes de Servicios de Comunicación Audiovisual (LSCA) y Argentina Digital (LAD).Mediante un decreto presidencial firmado el primer día de gobierno (Decreto 13\/2015), se creó el Ministerio de Comunicaciones, que absorbió funciones de las autoridades de aplicación de ambas normas. En los dos casos se trataba de entes autárquicos y descentralizados que luego de la promulgación del decreto perdieron características básicas que hacían a su independencia, como la personalidad jurídica propia, la asignación legal de recursos y la capacidad de administrarse a sí mismos, entre otras. Una semana más tarde el edificio de la Autoridad Federal de Servicios de Comunicación Audiovisual (AFSCA) ?autoridad de aplicación de la LSCA-, amaneció rodeado de policías que impedían el acceso de los directores, cuyo mandato se extendía hasta 2017. Ese mismo día se anunció la intervención por medio de otro decreto presidencial del organismo. Lo mismo ocurrió con el directorio de la Autoridad Federal de Tecnologías de la Información y la Comunicación, en los hechos de telecomunicaciones, AFTIC. Luego de la intervención de las autoridades de aplicación, y ya sobre el fin de año, un nuevo Decreto de Necesidad y Urgencia (267\/2015) avanzó con profundas reformas a la normativa vigente y dejó en evidencia que la nueva regulación de los servicios audiovisuales y de telecomunicaciones venía hecha a la medida de sus regulados.","palabras_clave":"Ley de Servicios de Comunicación Audiovisual |Derecho a la comunicación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4509,"idioma_id":5,"titulo":"AUTODEFINIDOS El sector audiovisual y el de telecomunicaciones cada vez más concentrados a partir del decreto 267\/2015","resumen":"Las empresas del sector de telecomunicaciones y de medios audiovisuales, que representan el capital concentrado y que reclaman la oportunidad de avanzar hacia un mercado cada vez más convergente, consiguieron una regulación hecha a su medida: el decreto 267\/2015. La celebración de las nuevas tecnologías, la remanida idea de la convergencia como milagro pluralizador del acceso al debate público y las referencias a internet y el siglo XXI no alcanzan para enmascarar una política pública que, tanto en su forma como en su contenido, violenta la institucionalidad republicana, los estándares internacionales de libertad de expresión y los principios esgrimidos por la mayoría de las fuerzas políticas de nuestro país durante el último lustro. El reciente decreto 267\/2015 firmado por el presidente Mauricio Macri transgrede sobradamente todos esos aspectos y deja en evidencia que la nueva regulación de los servicios audiovisuales y de telecomunicaciones está hecha a la medida de sus regulados, en particular de los representantes del capital concentrado y, puntualmente, de uno de ellos.Para poder dimensionar las transformaciones dispuestas resulta útil evaluar desde una perspectiva de derechos, aunque de un modo breve, la magnitud de los cambios en comparación con las leyes Argentina Digital (LAD) y de Servicios de Comunicación Audiovisual (LSCA).En primer lugar, debemos detenernos en que la Constitución Nacional distingue, en su artículo 75, inciso 19, que es facultad del Congreso Nacional legislar en lo que hace a la defensa y promoción del patrimonio audiovisual nacional. En los considerandos del decreto 267 no se menciona este inciso, por lo que evidentemente la vocación no es ni la defensa ni la promoción. Sin embargo, resulta central el análisis a la luz de los estándares de derechos humanos toda vez que el decreto invoca el inciso 22 de dicho artículo de la Carta Magna. Es decir, el rango constitucional de los tratados internacionales de derechos humanos.No nos detendremos aquí en la más o menos atinada aplicación de la norma en estos años, algo que excedería los objetivos del presente texto, sino que nos limitaremos a su redacción y alteraciones. Valga tan sólo señalar al respecto que entendemos que no pueden considerarse las recientes modificaciones simplemente como el desplazamiento de un péndulo o una inversión de una relación de fuerzas. No hay dos demonios.","palabras_clave":"Ley de servicuis de comuniación audiovisual"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4510,"idioma_id":4,"titulo":"Human Frataxin Folds Via an Intermediate State. Role of the C-Terminal Region","resumen":"The aim of this study is to investigate the folding reaction of human frataxin, whose deficiency causes the neurodegenerative disease Friedreich?s Ataxia (FRDA). The characterization of different conformational states would provide knowledge about how frataxin can be stabilized without altering its functionality. Wild-type human frataxin and a set of mutants, including two highly destabilized FRDA-associated variants were studied by urea-induced folding\/unfolding in a rapid mixing device and followed by circular dichroism. The analysis clearly indicates the existence of an intermediate state (I) in the folding route with significant secondary structure content but relatively low compactness, compared with the native ensemble. However, at high NaCl concentrations I-state gains substantial compaction, and the unfolding barrier is strongly affected, revealing the importance of electrostatics in the folding mechanism. The role of the C-terminal region (CTR), the key determinant of frataxin stability, was also studied. Simulations consistently with experiments revealed that this stretch is essentially unstructured, in the most compact transition state ensemble (TSE2). The complete truncation of the CTR drastically destabilizes the native state without altering TSE2. Results presented here shed light on the folding mechanism of frataxin, opening the possibility of mutating it to generate hyperstable variants without altering their folding kinetics.","palabras_clave":"HUMAN FRATAXIN|FOLDING KINETICS|INTERMEDIATE STATE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4511,"idioma_id":4,"titulo":"Controllability of protein-protein interaction phosphorylation-based networks. Participation of the hub 14-3-3 protein family.","resumen":"Posttranslational regulation of protein function is an ubiquitous mechanism in eukaryotic cells. Here, we analyzed biological properties of nodes and edges of a human protein-protein interaction phosphorylation-based network, especially of those nodes critical for the network controllability. We found that the minimal number of critical nodes needed to control the whole network is 29%, which is considerably lower compared to other real networks. These critical nodes are more regulated by posttranslational modifications and contain more binding domains to these modifications than other kinds of nodes in the network, suggesting an intra-group fast regulation. Also, when we analyzed the edges characteristics that connect critical and non-critical nodes, we found that the former are enriched in domain-to-eukaryotic linear motif interactions, whereas the later are enriched in domain-domain interactions. Our findings suggest a possible structure for protein-protein interaction networks with a densely interconnected and self-regulated central core, composed of critical nodes with a high participation in the controllability of the full network, and less regulated peripheral nodes. Our study offers a deeper understanding of complex network control and bridges the controllability theorems for complex networks and biological protein-protein interaction phosphorylation-based networked systems.","palabras_clave":"14-3-3|networks|controlability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4512,"idioma_id":4,"titulo":"Responses of multiple biomarkers in the fish Hoplosternum littorale after exposure to chromium and lead","resumen":"We studied the effect of chromium (Cr) and lead (Pb) and their combination using the freshwater fish Hoplosternum littorale. After 15 days, we analyzed the responses of multiple biomarkers, such as morphometric and hematological parameters, hepatic and renal enzymes activities (aspartate and alanine aminotransferases, alkaline phosphatase) and oxidative stress markers (antioxidant enzymes activities and lipid peroxidation). Cr caused a decrease in glucose levels and tissue damage in liver and kidney. Pb caused changes in hematological parameters, tissue damage in liver and kidney and induced liver lipid peroxidation. The metal combination modified hematological parameters and increased the hepatic injury biomarkers. According to multivariate statistical analysis, fish exposed to the mixture showed a differential physiologic profile to those exposed to individual metals. Thus, H. littorale has demonstrated to be sensitive for biomarkers response and suitable as potential test species. Our results suggest deleterious effects of sublethal concentrations of heavy metals and support the usefulness of a multi-biomarker approach for the characterization of toxicological mechanisms induced by the exposure to a combination of Cr and Pb.","palabras_clave":"FISH|HEAVY METALS|HEMATOLOGY|OXIDATIVE STRESS|TRANSAMINASES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4513,"idioma_id":4,"titulo":"Alterations in the general condition, biochemical parameters and locomotor activity in Cnesterodon decemmaculatus exposed to commercial formulations of chlorpyrifos, glyphosate and their mixtures","resumen":"The Pampean region, an extensive area of South America is continuously impacted by agricultural activities and the pesticides related to them like chlorpyrifos and glyphosate. Both pesticides have been registered in freshwater bodies of the region. One of the most abundant and widely distributed fish species in Pampean streams is Cnesterodon decemmaculatus, which have to cope with this altered scenario. In the present study the toxicity of Clorfox®and Roundup Max®, the commercial formulations of chlorpyrifos and glyphosate, respectively, and their mixture where evaluated using a set of biomarkers at different biological organization levels in fish exposed to relevant environmentally pesticides con-centrations. Somatic indexes such as the condition factor (K), and the hepato-somatic index (HSI), the locomotor activity through the distance traveled and the average speed, the enzymatic activities of acetyl-cholinesterase (AChE) in brain and muscle, catalase (CAT) in muscle and liver, glutathione-S-transferase(GST) in brain, liver, muscle and gills, aspartate amino-transferase (AST), alanine amino-transferase (ALT),AST\/ALT ratio and alkaline phosphatase (ALP) in liver were measured on C. decemmaculatus. Adult females were exposed during 6 weeks to the following concentrations: 0.0084 ul\/l and 0.00084 ul\/l of Clorfox(CF), 0.2 and 2 mg\/l of Roundup Max (RM) and all the combinations of these concentrations. The CF exposure caused a decrease in the condition factor and in the locomotor activity parameters and induced an increase brain AChE, liver CAT activity and AST\/ALT ratio. On the other hand, the exposure to RM produced a decrease in liver GST, AST\/ALT ratio and ALP activity. Finally, some pesticide combinations decrease general condition and liver GST activities, and increase brain GST and liver ALP activities. Different responses in biomarkers were observed in mixtures treatments, reflecting the complex interactions between these toxics and suggesting a suppressive action of RM on CF effects. Since the concentrations we tested are environmentally relevant and the overall fish health conditionwas affected, the presence of these pesticides in freshwater systems could impose a risk for populations by causing deleterious effects on C. decemmaculatus in Pampean region.","palabras_clave":"Biomarkers|Bioindicator|Fish|Pesticides|Freshwater Quality"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4514,"idioma_id":4,"titulo":"Differential expression and accumulation of 14-3-3 paralogs in 3T3-L1 preadipocytes and differentiated cells","resumen":"The 14-3-3 protein family interacts with more than 2000 different proteins in mammals, as a result of itsspecific phospho-serine\/phospho-threonine binding activity. Seven paralogs are strictly conserved inmammalian species. Here, we show that during adipogenic differentiation of 3T3-L1 preadipocytes, thelevel of each 14-3-3 protein paralog is regulated independently. For instance 14-3-3&#946;, &#947;, and &#951; proteinlevels are increased compared to untreated cells. In contrast, 14-3-3&#949; protein levels decreased afterdifferentiation while others remained constant. In silico analysis of the promoter region of each geneshowed differences that explain the results obtained at mRNA and protein levels.","palabras_clave":"diferenciación|3T3L1|14-3-3"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4515,"idioma_id":4,"titulo":"Allantoin accumulation mediated by allantoinase downregulation and transport by Ureide Permease 5 confers salt stress tolerance to Arabidopsis plants","resumen":"Allantoin, a metabolite generated in the purine degradation pathway, was primarily considered an intermediate for recycling of the abundant nitrogen assimilated in plant purines. More speci&#64257;cally, tropical legumes utilize allantoin and allantoic acid as major nodule-to-shoot nitrogen transport compounds. In other species, an increase in allantoin content was observed under different stress conditions, but the underlying molecular mechanisms remain poorly understood. In this work, Arabidopsis thaliana was used as a model system to investigate the effects of salt stress on allantoin metabolism and to know whether its accumulation results in plant protection. Plant seedlings treated with NaCl at different concentrations showed higher allantoin and lower allantoic acid contents. Treatments with NaCl favored the expression of genes involved in allantoin synthesis, but strongly repressed the unique gene encoding allantoinase (AtALN). Due to the potential regulatory role of this gene for allantoin accumulation, AtALN promoter activity was studied using a reporter system. GUS mediated coloration was found in speci&#64257;c plant tissues and was diminished with increasing salt concentrations. Phenotypic analysis of knockout, knockdown and stress-inducible mutants for AtALN revealed that allantoin accumulation is essential for salt stress tolerance. In addition, the possible role of allantoin transport was investigated. The Ureide Permease 5 (UPS5) is expressed in the cortex and endodermis of roots and its transcription is enhanced by salt treatment. Ups5 knockout plants under salt stress presented a susceptible phenotype and altered allantoin root-to-shoot content ratios. Possible roles of allantoin as a protectant compound in oxidative events or signaling are discussed.","palabras_clave":"Allantoin|Allantoinase|Ureide permease|Salt stress tolerance|Arabidopsis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4516,"idioma_id":4,"titulo":"The nuclear ribonucleoprotein SmD1 interplays with splicing, RNA quality control and post-transcriptional gene silencing in Arabidopsis","resumen":"RNA quality control (RQC) eliminates aberrant RNAs based on their atypical structure, whereas posttranscriptional genesilencing (PTGS) eliminates both aberrant and functional RNAs through the sequence-specific action of short interferingRNAs (siRNAs). The Arabidopsis thaliana mutant smd1b was identified in a genetic screen for PTGS deficiency, revealing theinvolvement of SmD1, a component of the Smith (Sm) complex, in PTGS. The smd1a and smd1b single mutants are viable, butthe smd1a smd1b double mutant is embryo-lethal, indicating that SmD1 function is essential. SmD1b resides in nucleoli andnucleoplasmic speckles, colocalizing with the splicing-related factor SR34. Consistent with this, the smd1b mutant exhibitsintron retention at certain endogenous mRNAs. SmD1 binds to RNAs transcribed from silenced transgenes butnot nonsilenced ones, indicating a direct role in PTGS. Yet, mutations in the RQC factors UPFRAMESHIFT3,EXORIBONUCLEASE2 (XRN2), XRN3, and XRN4 restore PTGS in smd1b, indicating that SmD1 is not essential for butrather facilitates PTGS. Moreover, the smd1b mtr4 double mutant is embryo-lethal, suggesting that SmD1 is essential formRNA TRANSPORT REGULATOR4-dependent RQC. These results indicate that SmD1 interplays with splicing, RQC, andPTGS. We propose that SmD1 facilitates PTGS by protecting transgene-derived aberrant RNAs from degradation by RQC inthe nucleus, allowing sufficient amounts to enter cytoplasmic siRNA bodies to activate PTGS.","palabras_clave":"SPLICING|PTGS|RNA QUALITY CONTROL|SMD1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4517,"idioma_id":4,"titulo":"Put your 3D glasses on: plant chromatin is on show","resumen":"The three-dimensional organization of the eukaryotic nucleus and its chromosomal conformation have emerged as important features in the complex network of mechanisms behind gene activity and genome connectivity dynamics, which can be evidenced in the regionalized chromosomal spatial distribution and the clustering of diverse genomic regions with similar expression patterns. The development of chromatin conformation capture (3C) techniques has permitted the elucidation of commonalities between the eukaryotic phyla, as well as important differences among them. The growing number of studies in the field performed in plants has shed light on the structural and regulatory features of these organisms. For instance, it has been proposed that plant chromatin can be arranged into different conformations such as Rabl, Rosette-like, and Bouquet, and that both short- and long-range chromatin interactions occur in Arabidopsis. In this review, we compile the current knowledge about chromosome architecture characteristics in plants, as well as the molecular events and elements (including long non-coding RNAs, histone and DNA modifications, chromatin remodeling complexes, and transcription factors) shaping the genome three-dimensional conformation. Furthermore, we discuss the developmental outputs of genome topology-mediated gene expression regulation. It is becoming increasingly clear that new tools and techniques with higher resolution need to be developed and implemented in Arabidopsis and other model plants in order to better understand chromosome architecture dynamics, from an integrative perspective with other fields of plant biology such as development, stress biology, and finally agriculture.","palabras_clave":"chormatin|DNA methylation|epigenetics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4518,"idioma_id":4,"titulo":"Approaches to unravel pathways of reactive oxygen species in the photoinactivation of Staphylococcus aureus induced by a dicationic fulleropyrrolidinium derivative","resumen":"The photodynamic mechanism sensitized by N,N-dimethyl-2-[4-(3-N,N,N-trimethylammoniopropoxy)phenyl]fulleropyrrolidinium (DPC602+) was investigated in Staphylococcus aureus cells. Different experimental conditions were used to detect reactive oxygen species (ROS) in S. aureus cell suspensions. First, a photoinactivation of 4 log decrease of S. aureus viability was chosen using 0.5 &#61549;M DPC602+ and 15 min irradiation. An anoxic atmosphere indicated that oxygen was required for an effective photoinactivation. Also, photoprotection was found in the presence of sodium azide, whereas the photocytotoxicity induced by DPC602+ increased in D2O. The addition of diazabicyclo[2.2.2]octane or D-mannitol produced a reduction in the S. aureus photokilling. Moreover, singlet molecular oxygen, O2(1&#61508;g), was detected by the reaction with 9,10-dimethylanthracene into the S. aureus cells. A decrease in the photoinactivation of S. aureus was observed in the presence of &#946;?nicotinamide adenine dinucleotide reduced form, which was dependent on the NADH concentration. Therefore, under aerobic condition the photocytotoxicity activity induced by DPC602+ was mediated by mainly a contribution of type II process. Moreover, photoinactivation of S. aureus was possible with DPC602+ in the presence of azide anions under anoxic condition. However, these conditions were not effective to photoinactivate Escherichia coli. On the other hand, the addition of potassium iodide produced an increase in the photokilling of bacteria, depending on the KI concentration and irradiation times. The formation of reactive iodine species may be contributing to inactivate S. aureus cells photoinduced by DPC602+.","palabras_clave":"FULLERENE|PHOTOSENSITIZER|PHOTODYNAMIC INACTIVATION|ROS|Staphylococcus aureus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4519,"idioma_id":4,"titulo":"The AIMSS Project - III. The stellar populations of compact stellar systems","resumen":"In recent years, a growing zoo of compact stellar systems (CSSs) havebeen found whose physical properties (mass, size, velocity dispersion)place them between classical globular clusters (GCs) and true galaxies,leading to debates about their nature. Here we present results using aso far underutilized discriminant, their stellar population properties.Based on new spectroscopy from 8-10m telescopes, we derive ages,metallicities, and [&#945;\/Fe] of 29 CSSs. These range from GCs withsizes of merely a few parsec to compact ellipticals (cEs) larger thanM32. Together with a literature compilation, this provides a panoramicview of the stellar population characteristics of early-type systems. Wefind that the CSSs are predominantly more metal rich than typicalgalaxies at the same stellar mass. At high mass, the cEs depart from themass-metallicity relation of massive early-type galaxies, which forms acontinuous sequence with dwarf galaxies. At lower mass, the metallicitydistribution of ultracompact dwarfs (UCDs) changes at a few times107 M&#8857;, which roughly coincides with the masswhere luminosity function arguments previously suggested the GCpopulation ends. The highest metallicities in CSSs are paralleled onlyby those of dwarf galaxy nuclei and the central parts of massive earlytypes. These findings can be interpreted as CSSs previously being moremassive and undergoing tidal interactions to obtain their current massand compact size. Such an interpretation is supported by CSSs withdirect evidence for tidal stripping, and by an examination of the CSSinternal escape velocities.","palabras_clave":"galaxies|fundamental parameters|stellar content"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4520,"idioma_id":4,"titulo":"Occurrence of fungicide residues on Argentinean blueberry fruit and juice samples","resumen":"Azoxystrobin, boscalid, cyprodinil, fludioxonil and pyraclostrobin are fungicides commonly used in Argentina against different fungus contaminations in the blueberry field. The presence of these fungicides was investigated in 50 samples of blueberry fruit and 15 samples of blueberry juice purchased in Argentina. Fungicide residues were determined by solid-phase microextraction (SPME) coupled to gas chromatography with micro-electron capture and nitrogen phosphorous detector. The average concentrations of azoxystrobin were 48 &#61549;g\/kg in blueberry fruit. Average of boscalid were 43 and 239 &#61549;g\/kg, of cyprodinil 1581 and 852 &#61549;g\/kg, of fludioxonil 1077 and 2842 g\/kg, and of pyraclostrobin 578 and 3414 &#61549;g\/kg, in fruits and juice respectively. The higher concentrations of fungicides were found in those cases where the time between the application of the compound and the fruit?s commercialization was shorter than the fungicide?s half-life.","palabras_clave":"FUNGICIDES|BLUEBERRIES|Time between fungicide application and harvest|SPME\/GC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4521,"idioma_id":5,"titulo":"Reducción del Contenido de Insecticidas en Musculo de Pescado por Efecto de la Cocción","resumen":"La contaminación de los recursos naturales con pesticidas genera preocupación debido a los efectos que podría traer a la salud de los seres humanos y animales. Compuestos organoclorados, como el &#945;-endosulfan y el aldrin, podrían tener como destino ambiental los cursos de agua y de esta manera contaminar los peces. El armado, Oxyidoras kneri, es un pez común en el río Uruguay. Los objetivos del presente trabajo son la validación de una metodología para la determinación de estos compuestos y la determinación del efecto de la cocción en el contenido inicial de los mismos en el tejido muscular de pescado. El método extractivo empleado para la validación de la metodología analítica fue QuEChERS (QuickEasy Cheap Effective Rugged Safe). Los analitos se determinaron por cromatografía gaseosa con detector de microcaptura de electrones (µ-ECD). La calibración se realizó con muestra adicionada debido a la existencia de efecto matriz. La curva de calibración con matriz adicionada resultó lineal en el rango de 0,01 a 0,5 mg\/l. La linealidad del método es muy buena, con R2 superiores a 0,99. Se evaluó la precisión a 0,1 y 0,5 mg\/l, siendo la desviación estándar relativa (RSD) menor al 10% para n=5. La recuperación se estudió para n=3 a las mismas concentraciones, estando todas entre 95 y 108%. Los límites de detección y cuantificación fueron de 19,14 y 63,79µg g\/kg de muestra para &#945;-endosulfan y de 18,43y 61,44µg g\/kg para aldrin, respectivamente. Una vez finalizada la validación de la metodología, se estudió el efecto de la cocción sobre la concentración inicial de los pesticidas en músculo de pescado.El estudio se llevó a cabo por triplicado, utilizando un horno eléctrico y una termocupla a fin de verificar que la temperatura en el centro térmico de las muestras sea de 71°C. Posteriormente, se determinó la concentración de cada compuesto en las distintas muestras. El porcentaje de destrucción térmica durante el proceso de cocción fue de 45% para el &#945;-endosulfan, pero no se observó ningún efecto sobre el aldrin. El análisis estadístico de los resultados indicó que el método es lineal y presenta alta sensibilidad, exactitud y precisión, siendo útil para la determinación de compuestos organoclorados en músculo de pescado. Se puede concluir que la cocción a 71°C en el centro térmico asegura la eliminación parcial de &#945;-endosulfan, no teniendo efecto sobre aldrin. Se considera relevante continuar la investigación a fin de evaluar el impacto que tendrán condiciones diferentes de cocción, permitiendo optimizar los resultados.","palabras_clave":"FUNGICIDAS CLORADOS|PESCADO|COCCION|QuEChERS\/GC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4522,"idioma_id":4,"titulo":"Exploring nest webs in more detail to improve forest management","resumen":"The interactions between birds that use tree cavities for breeding, roosting and sheltering have been named ?nest webs?. We applied for the first time in nest-web studies some tools developed in network theory, in order to develop conservation and management recommendations of forest biodiversity. We recorded 109 interactions between 15 bird and 11 cavity-bearing tree species, in a subtropical piedmont forest (PF) of northwestern Argentina. Bird species in this nest-web included four woodpecker species, whose cavities were scarcely (9%) used by non-excavator birds, such as parrots, owls, and woodcreepers. Based on the Importance and Strength indices the most important tree species were Calycophyllum mul- tiflorum (Rubiaceae) and Anadenanthera colubrina (Fabaceae). The nest web contained three main inter- action modules: one composed by woodpeckers interacting with both living and standing dead trees; another by non-excavator birds using decay-formed cavities in living trees; and a third small module that had a few birds using woodpecker-excavated cavities in living trees. Important tree species were differ- ent for woodpecker and non-excavator modules. Extinction simulation of the most important tree species tripled the negative impact on cavity-using bird assemblage compared with the random extinction of tree species. In logging operations special consideration should be taken to ensure the maintenance of key tree species for the conservation of all the nest-web components in PF.","palabras_clave":"Cavity-using birds|Interaction networks|Modules|Forest management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4523,"idioma_id":4,"titulo":"IS ANAEROBIC MINERALIZABLE NITROGEN SUITABLE AS SOIL HEALTH INDICATOR?","resumen":"Soil organic matter (SOM) and especially its labile fractions such as particulate organic matter (POM) dynamics are very sensitive to soil use and strongly influence soil ecosystem services. Particulate organic matter has been proposed as a soil quality\/health indicator but its determination is tedious and time consuming (i.e. manhours). Anaerobic mineralizable nitrogen (AN) is closely related to soil organic fraction and is very easily determined. Therefore, we proposed to evaluate AN as soil quality\/health indicator through the assessment of its relationship with SOM, POM, soil aggregate stability (AS), and maize (Zea mays, L.) relative yield (RY) under different long term soil use for cropping al Balcarce, Argentina (37º 45? 14?? S; 58º 17? 52?? W). Soil samples had been taken at two depths (0-5 and 5-20 cm) in the fall of 1998, 2000, 2003, 2006, 2009, and 2012 from a long term tillage system (TS, conventional (CT) and no-tillage (NT)) and nitrogen fertilization (NF, with and without nitrogen as fertilizer) experiment on a complex of Typic and Petrocalcic Argiudolls. Carbon content in SOM (SOC) and in POM (POC), and AN were determined in all soil samples, whereas AS was determined in other soil samples taken in 2006, 2009 and 2012 from the arable layer (0-20 cm). Regardless TS and NF, SOC, POC, and AN decreased with time under cropping at both 5-20 and 0-20 cm. In the uppermost layer (0-5 cm) decreases of all three variables were observed only under CT. Anaerobically mineralized nitrogen variation related to SOC (R2 0.59 - 0.78, P<0.05) and especially POC (R2 0.80 - 0.85, P<0.05) variations. Likewise, changes in maize RY related better (R2 0.92 and 0.95 (P<0.05) for CT and NT, respectively) to variation in AN, than to SOC and POC variations. Besides, change in aggregate mean weigh diameter (&#61508;MWD) acceptably related to AN at 0-20 cm (R2=0.67, P<0.05) and much better at 0-5 cm (R2=0.86, P<0.05). Both coefficients of determination were higher than those obtained relating &#61508;MWD with SOC or POC. Given the easiness of its determination, its sensitiveness, and that it relates to the variation of different key soil parameters and crop behavior, AN could be proposed as an effective soil quality\/health indicator. However, studies should be carried on taking into account a broader range of soil and management situations to validate the trends observed in this work.","palabras_clave":"aggregate stability|anaerobic nitrogen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4525,"idioma_id":4,"titulo":"First Simultaneous Views of the Axial and Lateral Perspectives of a Coronal Mass Ejection","resumen":"The different appearances exhibited by Coronal Mass Ejections (CMEs) are believed to be, in part, the result of different orientations of their main axis of symmetry, consistent with a flux-rope configuration. There are observational reports of CMEs seen along their main axis (axial perspective) and perpendicular to it (lateral perspective), however no simultaneous observations of both perspectives from the same CME have been reported to date. The stereoscopic views of the telescopes aboard the Solar-Terrestrial Relations Observatory (STEREO) twin spacecraft, in combination with the views from the Solar and Heliospheric Observatory (SOHO) and the Solar Dynamics Observatory (SDO), allow us to study the axial and lateral perspectives of a CME simultaneously for the first time. In addition, this study shows that the lateral angular extent (L) increaseslinearly with time, while the angular extent of the axial perspective (D) presents this behavior only from the low corona to &#8776; 5 R , where it slows down. The ratio L\/D &#8776; 1.6 obtained here as the average over several points in time is consistent with measurements of L and D previously performed on events exhibiting only one of the perspectives from the single vantage point provided by SOHO.","palabras_clave":"CMES, INITIATION AND PROPAGATION|CMES, LOW CORONAL SIGNATURES|PROMINENCES, DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4526,"idioma_id":4,"titulo":"Analysis of neuronal nicotinic acetylcholine receptor &#945;4&#946;2 activation at the single-channel level","resumen":"The neuronal nicotinic acetylcholine receptor &#945;4&#946;2 forms pentameric proteins with two alternate stoichiometries. The high-sensitivity receptor is related to (&#945;4)2(&#946;2)3 stoichiometry while the low-sensitivity receptor to (&#945;4)3(&#946;2)2 stoichiometry. Both subtypes share two binding sites at the &#945;4(+)\/&#946;2(-) interface with high affinity for agonists. (&#945;4)3(&#946;2)2 has an additional binding site at the &#945;4(+)\/&#945;4(-) interface with low affinity for agonists. We investigated activation kinetics of both receptor subtypes by patch-clamp recordings of single-channel activity in the presence of several concentrations of acetylcholine (0.5 to 300 µM). We used kinetic software to fit these data with kinetic models. We found that the high-sensitivity subtype correlates with the low-conductance channel (g-70=29 pS) and does not activate with high efficacy. On the contrary, the low-sensitivity subtype correlated with a high-conductance channel (g-70=44 pS) and exhibited higher activation efficacy. Opening events of individual nAChRs at high agonist concentrations occurred in clusters, which allowed us to determine kinetic constants for the activation of the triliganded receptor. Our kinetic modeling identified an intermediate state, between resting and open conformation of the receptor. Binding of the third molecule increases the efficacy of receptor activation by favoring the transition between resting and intermediate state around 18 times. The low rate for this transition in the diliganded receptor explains the action of acetylcholine as partial agonist when it binds to the high-affinity sites. The presence of the third binding site emerges as a potent modulator of nicotinic receptor &#945;4&#946;2 activation which may display different functions depending on agonist concentration.","palabras_clave":"NICOTINIC|RECEPTOR|ALPHA4BETA2|KINETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4527,"idioma_id":4,"titulo":"Progesterone modulates pro-inflammatory cytokine expression profile after spinal cord injury: Implications for neuropathic pain","resumen":"Ver version completa en Full Text","palabras_clave":"CYTOKIN|NMDA RECEPTOR|SPINAL CORD|NEUROSTEROID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4528,"idioma_id":4,"titulo":". Neuroactive steroids, nociception and neuropathic pain: a flashback to go forward.","resumen":"The present review discusses the potential role of neurosteroids \/ neuroactive steroids in the regulation of nociceptive and neuropathic pain, and recapitulates the current knowledge on the main mechanisms involved in the reduction of pain, especially those occurring at the dorsal horn of the spinal cord, a crucial site for nociceptive processing. We will make special focus on progesterone and its derivative allopregnanolone, which have been shown to exert remarkable actions in order to prevent or reverse the maladaptive changes and pain behaviors that arise after nervous system damage in various experimental neuropathic conditions.","palabras_clave":"NEUROPATHIC PAIN|PROGESTERONE|ALLOPREGNANOLONE|THERAPEUTIC STRATEGIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4529,"idioma_id":4,"titulo":"Identification and characterization of contrasting sunflower genotypes to early leaf senescence process combining molecular and physiological studies (Helianthus annuus L.)","resumen":"AbstractLeaf senescence is a complex mechanism ruled by multiple genetic and environmental variables that affect crop yields. It is the last stage in leaf development, is characterized by an active decline in photosynthetic rate, nutrients recycling and cell death. The aim of this work was to identify contrasting sunflower inbred lines differing in leaf senescence and to deepen the study of this process in sunflower. Ten sunflower genotypes, previously selected by physiological analysis from 150 inbred genotypes, were evaluated under field conditions through physiological, cytological and molecular analysis. The physiological measurement allowed the identification of two contrasting senescence inbred lines, R453 and B481-6, with an increase in yield in the senescence delayed genotype. These findings were confirmed by cytological and molecular analysis using TUNEL, genomic DNA gel electrophoresis, flow sorting and gene expression analysis by qPCR. These results allowed the selection of the two most promising contrasting genotypes, which enables future studies and the identification of new biomarkers associated to early senescence in sunflower. In addition, they allowed the tuning of cytological techniques for a non-model species and its integration with molecular variables.","palabras_clave":"HELIANTHUS ANNUUS|LEAF SENESCENCE|FLOW CYTOMETRY|PHYSIOLOGICAL ANALYSYS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4530,"idioma_id":4,"titulo":"Melanism and ontogeny: A case study in lizards of the Liolaemus fitzingerii group (Squamata: Liolaemini)","resumen":"Color polymorphisms in general and melanism in particular have been the focus of many evolutionary adaptation studies. In lizards of the genus Liolaemus, patterns of melanism have been poorly studied, although they have been used as diagnostic characters for identifying and describing new species. We investigated the relationships between melanism, body size, sex and time in a Liolaemus xanthoviridis (Cei and Scolaro 1980), population with extensive ventral melanism. The study took place in Bahía Isla Escondida, Chubut (Argentina) during three summer seasons (2012 to 2014). We tagged each individual, recorded body measurements and sex, and took ventral photographs to estimate the proportion of melanism. Our results showed that ventral melanism increased over time as each individual increased its snout vent length (SVL). Body size explained 44% of the variation in melanism, and males were more melanistic than females. Previous comparative studies of lizards in this species group showed no relationship between melanism and different taxonomic units or with thermal functionality. Here, we present evidence suggesting that melanism might be a character with an ontogenetic origin that is strongly associated with sex and body size. This pattern could be shared among species of this group of lizards, and even more importantly it may be related to variable selection forces occurring throughout ontogeny.","palabras_clave":"LIOLAEMUS XANTHOVIRIDIS|RAWSON  LIZARD|IMAGE ANALYSIS|SEX|SIZE|VENTRAL MELANISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4531,"idioma_id":5,"titulo":"Morfologia lineal y geometrica en un grupo de lagartijas patagonicas del genero Phymaturus (Squamata, Liolaemini)","resumen":"ASDG","palabras_clave":"LIMITES DE ESPECIES|VARIACION|MORFOMETRIA GEOMETRICA|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4532,"idioma_id":4,"titulo":"The fate of memory: Reconsolidation and the case of Prediction Error","resumen":"The ability to make predictions based on stored information is a general coding strategy. A Prediction-Error (PE) is a mismatch between expected and current events. It was proposed as the process by which memories are acquired. But, our memories like ourselves are subject to change. Thus, an acquired memory can become active and update its content or strength by a labilization-reconsolidation process. Within the reconsolidation framework, PE drives the updating of consolidated memories. Moreover, memory features, such as strength and age, are crucial boundary conditions that limit the initiation of the reconsolidation process. In order to disentangle these boundary conditions, we review the role of surprise, classical models of conditioning, and their neural correlates. Several forms of PE were found to be capable of inducing memory labilization-reconsolidation. Notably, many of the PE findings mirror those ofmemory-reconsolidation, suggesting a strong link between these signals and memory process. Altogether, the aim of the present work is to integrate a psychological and neuroscientific analysis of PE into a general framework for memory-reconsolidation.","palabras_clave":"dopamine|expectation|hippocampus|prediction error|memory labilization|memory reactivation|memory reconsolidation|memory strenghening|memory updating|reminder|surprise"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4533,"idioma_id":4,"titulo":"GLYCO-NANO-ONCOLOGY: NOVEL THERAPEUTIC OPPORTUNITIES BY COMBINING SMALL AND SWEET","resumen":"Recent efforts toward defining the molecular features of the tumor microenvironment have revealed dramatic changes in the expression of glycan-related genes including glycosyltransferases and glycosidases. These changes affect glycosylation of proteins and lipids not only in cancer cells themselves, but also in cancer associated-stromal, endothelial and immune cells. These glycan alterations including increased frequency of &#946;1,6-branched N-glycans and bisecting N-glycans, overexpression of tumor-associated mucins, preferred expression of T, Tn and sialyl-Tn antigen and altered surface sialylation, may contribute to tumor progression by masking or unmasking specific ligands for endogenous lectins, including members of the C-type lectin, siglec and galectin families. Differential expression of glycans or glycan-binding proteins could be capitalized for the identification of novel biomarkers and might provide novel opportunities for therapeutic intervention. This review focuses on the biological relevance of lectin-glycan interactions in the tumor microenvironment (mainly illustrated by the immunosuppressive and pro-angiogenic activities of galectin-1) and the design of functionalized nanoparticles for pharmacological delivery of multimeric glycans, lectins or selective inhibitors of lectin-glycan interactions with antitumor activity.","palabras_clave":"GLICOBIOLOGÍA|CANCER|GALECTINA|TERASNÓSTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4534,"idioma_id":4,"titulo":"The mathematical whisker: A review of numerical models of the rat&#1523;s vibrissa biomechanics","resumen":"The vibrissal system of the rat refers to specialized hairs the animal uses for tactile sensory perception. Rats actively move their whiskers in a characteristic way called ?whisking?. Interaction with the environment produces elastic deformation of the whiskers, generating mechanical signals in the whisker?follicle complex. Advances in our understanding of the vibrissal complex biomechanics is of interest not only for the biological research field, but also for biomimetic approaches. The recent development of whisker numerical models has contributed to comprehending its sophisticated movements and its interactions with the follicle. The great diversity of behavioral patterns and complexities of the whisker?follicle ensemble encouraged the creation of many different biomechanical models. This review analyzes most of the whisker biomechanical models that have been developed so far. This review was written so as to render it accessible to readers coming from different research areas.","palabras_clave":"VIBRISSAL SYSTEM|WHISKER NUMERICAL MODEL|BIOMECHANICS|VIBRISSAL SHAFT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4535,"idioma_id":4,"titulo":"Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-&#945;-L-rhamnosyl-&#946;-D-glucosidase active on flavonoids","resumen":"Bacteria represent an underexplored source of diglycosidases. Twenty-five bacterial strains from the genera Actinoplanes, Bacillus, Corynebacterium, Microbacterium and Streptomyces were selected for their ability to grow in diglycosylated flavonoid-based media. The strains Actinoplanes missouriensis and Actinoplanes liguriae exhibited hesperidin deglycosylation activity (6-O-&#945;-L-rhamnosyl-&#946;-D-glucosidase activity, EC 3.2.1.168), which was 3 to 4 orders of magnitude higher than the corresponding monoglycosidase activities. The diglycosidase production was confirmed in A. missouriensis by zymographic assays and NMR analysis of the released disaccharide, rutinose. The gene encoding the 6-O-&#945;-L-rhamnosyl-&#946;-D-glucosidase was identified in the genome sequence of A. missouriensis 431T (GenBank accession number BAL86042.1) and functionally expressed in Escherichia coli. The recombinant protein hydrolyzed hesperidin and hesperidin methylchalcone, but not rutin, which indicates its specificity for 7-O-rutinosylated flavonoids. The protein was classified into the glycoside hydrolase family 55 (GH55) in contrast to the known eukaryotic diglycosidases, which belong to GH1 and GH5. These findings demonstrate that organisms other than plants and filamentous fungi can contribute to an expansion of the diglycosidase toolbox.","palabras_clave":"ALPHA-L-RHAMNOSIDASE|BETA-D-GLUCOSIDASE|INVERTING GLYCOSIDASE|METHYLUMBELLIFERYL-BETA-D-RUTINOSIDE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4536,"idioma_id":4,"titulo":"?Reconsolidation-induced memory persistence: participation of late phase hippocampal ERK activation","resumen":"Persistence is an attribute of long-term memories (LTM) that has recently caught researcher´s attention in search for mechanisms triggered by experience that assure memory perdurability. Up-to-date, scarce evidence of relationship between reconsolidation and persistence has been described. Here, we characterized hippocampal ERK participation in LTM reconsolidation and persistence using an inhibitory avoidance task (IA) at different time points. Intra-dorsalhippocampal(dHIP) administration of an ERK inhibitor (PD098059, PD, 1.0 &#956;g\/hippocampus) 3 h after retrieval did not affect reconsolidation of a strong IA, when tested 24 h apart. However, the same manipulation impaired performance when animals were tested at 7 days, regardless of the training´s strength; and being specific to memory reactivation. To the best of our knowledge, this is the first report showing that persistence might be triggered after memory reactivation involving an ERK\/MAPK-dependent process.","palabras_clave":"ERK\/MAPK|INHIBITORY AVOIDANCE|MEMORY RCONSOLIDATION|MEMORY PERSISTENCE|PD098059|MICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4537,"idioma_id":4,"titulo":"Lovász-Schrijver PSD-operator on Claw-Free Graphs","resumen":"The subject of this work is the study of the Lov´asz-SchrijverPSD-operator LS+ applied to the edge relaxation ESTAB(G) of the stableset polytope STAB(G) of a graph G.We are interested in the problemof characterizing the graphs G for which STAB(G) is achieved in one iterationof the LS+-operator, called LS+-perfect graphs, and to find an appropriatepolyhedral relaxation of STAB(G) that coincides with LS+(G)and STAB(G) if and only if G is LS+-perfect. An according conjecturehas been recently formulated (LS+-Perfect Graph Conjecture); here weverify it for the well-studied class of claw-free graphs.","palabras_clave":"STABLE SET POLYTOPE|N+-PERFECT GRAPHS|CLAW FREE GRAPHS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4538,"idioma_id":4,"titulo":"Morphology of juvenile stages of Kuschelina bergi (Harold) with biological information (Coleoptera, Chrysomelidae, Alticini)","resumen":"Kuschelina bergi (Harold, 1881) is being studied to be evaluated as a natural enemy of Phyla nodiflora var. minor (Hook.) N. O´ Leary & Múlgura (Verbenaceae), an invasive weed in Australia. Eggs, and 1st and 3rd instar larvae are described and illustrated for the first time. The following characters distinguish Kuschelina bergi: presence of two medial setae in prosternum, mesosternum and metasternum, absence of tubercle on sternum I and eight setae in abdominal segment IX. The 3rd instar larvae of K. bergi resemble Kuschelina gibbitarsa (Say) larvae: the body shape and details of mouthparts are similar, but the morphology of the mandible is different, as is the tarsungulus which has a single seta. Differences between K. bergi and other known larvae of Oedionychina are discussed. New biological data based on laboratory rearing and field observation are also presented and discussed.","palabras_clave":"GALERUCINAE|ALTICINI|FLEA BEETLE|BIOLOGICAL CONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4539,"idioma_id":4,"titulo":"Occurrence of Astyanax dissensus Lucena & Thofehrn, 2013 (Teleostei: Characidae) in Argentina","resumen":"The occurrence of Astyanax dissensus in theUruguay River, at Yapeyú, Corrientes, Argentina isreported for the first time in the country.","palabras_clave":"biogeography|Characiformes| species distribution|Uruguay River"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4540,"idioma_id":4,"titulo":"First record of the banjo catfish Bunocephalus doriae Boulenger 1902 (Siluriformes: Aspredinidae) in the Bermejo River basin, Salta, Argentina","resumen":"Intense sampling in the upper Bermejo River basin revealed the presence of specimens of the aspredinid genus Bunocephalus. After detailed morphological and morphometric analyses specimens were identified as Bunocephalus doriae. This is the first record of any member of the Aspredinidae in the upper Bermejo River basin.","palabras_clave":"YUNGAS|WIDE DISTRIBUTION|OSTARIOPHYSI|POPULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4541,"idioma_id":4,"titulo":"Runx1 and Foxp3 interplay regulates expression of breast cancer related genes","resumen":"Runx1 participation in epithelial mammary cells is still under review. Emergingdata indicates that Runx1 could be relevant for breast tumor promotion.However, to date no studies have specifically evaluated the functionalcontribution of Runx1 to control gene expression in mammary epithelial tumorcells. It has been described that Runx1 activity is defined by protein contextinteraction. Interestingly, Foxp3 is a breast tumor suppressor gene. Here weshow that endogenous Runx1 and Foxp3 physically interact in normalmammary cells and this interaction blocks Runx1 transcriptional activity.Furthermore we demonstrate that Runx1 is able to bind to R-spondin 3(RSPO3) and Gap Junction protein Alpha 1 (GJA1) promoters. This bindingupregulates Rspo3 oncogene expression and downregulates GJA1 tumorsuppressor gene expression in a Foxp3-dependent manner. Moreover,reduced Runx1 transcriptional activity decreases tumor cell migrationproperties. Collectively, these data provide evidence of a new mechanism forbreast tumor gene expression regulation, in which Runx1 and Foxp3physically interact to control mammary epithelial cell gene expression fate.Our work suggests for the first time that Runx1 could be involved in breasttumor progression depending on Foxp3 availability.","palabras_clave":"Runx1|RSPO3|Foxp3|breast cancer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4542,"idioma_id":4,"titulo":"Generation of thermal scattering libraries for liquids beyond the Gaussian approximation using molecular dynamics and NJOY\/LEAPR","resumen":"In this paper we review the existing methodologies for the generation of thermal scattering libraries for liquids from molecular dynamics simulations, either by Fourier transform or developing models in the Gaussian approximation. To overcome the limitations of the Gaussian approximation in the quasielastic range, we present a modification introduced to the LEAPR module of NJOY to include a random jump diffusion model, and a methodology to obtain the required parameters. This methodology is applied to light water with good results.","palabras_clave":"Thermal neutron scattering|Nuclear data|Light water"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4543,"idioma_id":4,"titulo":"Heterogeneous hydrogenation of nitroaromatic compounds on gold catalysts: Influence of titanium substitution in MCM-41 mesoporous supports","resumen":"A series of Ti-modified MCM-41 supports and their respective Au catalysts were prepared and characterized to study the effect of titanium into the support and on the characteristics of the Au surface species for heterogeneous hydrogenation of aromatic nitrocompounds. The systems were characterized by X-ray diffraction, Infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), diffuse reflectance UV?vis (DRUV?vis), N2 adsorption?desorption isotherms, ICP-MS and X-ray photoelectron spectroscopy (XPS). Additionally, these catalysts were tested for the hydrogenation of nitrobenzene in a batch-type reactor using ethanol as the solvent at 25 °C. Based on a combined FT-IR and DRUV?vis was possible to conclude that the Ti(IV) is incorporated into the framework of MCM-41 and Ti-containing MCM-41 catalysts were more active than an MCM-41 unmodified catalyst. The Ti(X)-MCM-41 supports prepared with Ti 2.0 wt% incorporation showed the best catalytic performance, which was attributed to an optimal number of isolated Ti sites that increased the Au stability on the support surface. For this system, the effect of the H2 pressure, solvent nature and recyclability of the catalyst was also studied. Finally, a quantitative structure property relationship (QSPR) model was obtained for the catalytic activities of para-substituted nitrobenzenes. The multilinear model considered two parametric descriptors: the sigma constant (&#963;) and the hydrophobic &#960;-constant, which account for the electronic and hydrophobic effect of the substituents.","palabras_clave":"Ti-MCM-41 SUPPORTS|GOLD CATALYST|NITROBENZENE-COMPOUNDS HYDROGENATION|QSPR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4544,"idioma_id":4,"titulo":"Nature of active vanadium nanospecies in MCM-41 type catalysts for olefins oxidation","resumen":"A multi-technique physicochemical investigation including UVeVis-DRS, Raman spectroscopy, XPS, ESR and FTIRS with pyridine adsorption was performed to analyze the nature of different vanadium nanospecies present on MCM-41 type catalysts. By employing a direct hydrothermal synthesis, vanadium species were incorporated into siliceous structure mainly as tetrahedrally coordinated isolated Vdþ ions, which would be located inside the wall and on the wall surface of the mesoporous channels. The coexistence of both vanadium oxidation states V4þ and V5þ was also revealed. Acidity measurements permitted to infer about the majority presence of Lewis acid sites, which increase with vanadium content. The catalytic performance of these materials was evaluated in the reaction of a-pinene oxidation with H2O2. The highest intrinsic activity of the sample with lower V loading was attributed to the high dispersion and efficiency of the isolated Vdþ species that actuate as active sites. A mixture of reactionproducts arising from competitive processes of epoxidation and allylic oxidation was found.","palabras_clave":"AMORPHOUS  MATERIALS|NANOSTRUCTURES|SURFACE PROPERTIES|OXIDATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4545,"idioma_id":4,"titulo":"Individual precocity, temporal persistence, and task specialisation of hygienic bees from selected colonies of Apis mellifera","resumen":"Hygienic behaviour is a complex trait that gives Apis mellifera L. resistance against brooddiseases. Variability in the expression of hygienic behaviour is evidenced at the colonyleveland is explained by the proportion and propensity of individual worker bees thatengage in hygienic activities. We investigated the temporal performance and the dynamicsof task-specialisation of individual bees over time, both in selected hygienic (H) andnon-hygienic (NH) colonies. Then we evaluated the impact of these behavioural aspectson the colony performance. Bees that perform hygienic behaviour (hygienic bees) in ourH colonies were more persistent in the hygienic activities throughout the days of theinvestigation. Such bees were more efficient in the removal of pin-killed brood than hygienicbees in the NH colonies. Hygienic bees in the H colonies were also specialist in thesub-tasks involved in the detection of odour stimulus from dead brood and continuedto perform these activities throughout the days of the investigation (temporal persistence).Age-distribution of hygienic bees in the H colonies was asymmetrical, with a largerproportion of these bees performing hygienic activities early in life. At a colony-level, Hshowed higher efficiency compared to the NH colonies. The present results highlight thefact that individual behaviour may influence the collective dynamics of the hygienic behaviourin honeybee colonies. The results also note that the selection for highly hygieniccolonies would result in changes in individual bees that improve the performance of thebehaviour at the colony level. The relevance of task-partitioning and age-specialisationof hygienic bees on social immunity is discussed","palabras_clave":"honeybees|disease tolerance|social immunity|task-specialisation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4546,"idioma_id":4,"titulo":"Deformation and fracture behavior of physical gelatin gel systems","resumen":"Food scientists usually used biopolymer physical gels as model systems because they are structurally and mechanically similar to many gel-like food products. In this paper, eight gelatin gel systems with different stiffness were prepared by varying gelatin concentration (10e30%w\/w), collagen source (bovine\/porcine) and solvent composition (0\/40%w\/w glycerol\/buffer mixture). The swelling behavior was evaluated and the mechanical response was characterized through puncture tests, uniaxial compression experiments and wire cutting fracture tests. From these tests, apparent gel strength, first order Ogden constitutive parameters (shear modulus, m, and strain hardening capability, a) and fracturetoughness (Gc) were determined. Samples that display apparent gel strength and swelling behaviorconsistent with a more physically cross-linked structure exhibit larger m and Gc and lower a values. It is shown that a and Gc are related with m independently of gelatin concentration, collagen source andglycerol presence. a decreases exponentially with increasing m whereas Gc increases linearly with m. The found experimental trends suggest that in the quasi-static range the overall mechanical behavior of gelatin gel systems is mainly controlled by the initial shear modulus, which is a direct measure of gelstiffness.","palabras_clave":"GELATIN GELS|SHEAR MODULUS|STRAIN HARDENING|FRACTURE TOUGHNESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4548,"idioma_id":4,"titulo":"Thermal response to the surface heat flux in a macrotidal coastal region (Nuevo Gulf, Argentina)","resumen":"At mid-latitudes, sea water temperature shows a strong seasonal cycle forced by the incident surface heat flux. As depth decreases, the heat flux incidence is damped by the horizontal flux, which prevents the indefinite growth of the seasonal temperature range. In the present work, cross-shore transport in the west coast of Nuevo Gulf (Argentina) was analyzed. Processes tending to cool the coastal waters in summer and to warm the coastal waters in winter, were identified through temperature measurements, surface heat flux and tidal height. The simplified models proposed here provide a feedback mechanism that links changes in surface heat flux with changes in the horizontal heat flux during both seasons. On shorter time scales, tide produces significant variations in the height of the water column, therefore influencing temperature fluctuations and the direction of the horizontal flow.","palabras_clave":"CROSS-SHORE EXCHANGE|DIRUNAL TEMPERATURE VARIABILITY|TIDAL CYCLE|SEASONAL CYCLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4549,"idioma_id":4,"titulo":"Novel vanadium-loaded ordered collagen scaffold promotes osteochondral differentiation of bone marrow progenitor cells","resumen":"Bone and cartilage regeneration can be improved by designing a functionalized biomaterial that includes bioactive drugs in a biocompatible and biodegradable scaffold. Based on our previous studies, we designed a vanadium-loaded collagen scaffold for osteochondral tissue engineering. Collagen-vanadium loaded scaffolds were characterized by SEM, FTIR, and permeability studies. Rat bone marrow progenitor cells were plated on collagen or vanadium-loaded membranes to evaluate differences in cell attachment, growth and osteogenic or chondrocytic differentiation. The potential cytotoxicity of the scaffolds was assessed by the MTT assay and by evaluation of morphological changes in cultured RAW 264.7 macrophages. Our results show that loading of VOAsc did not alter the grooved ordered structure of the collagen membrane although it increased membrane permeability, suggesting a more open structure. The VOAsc was released to the media, suggesting diffusion-controlled drug release. Vanadium-loaded membranes proved to be a better substratum than for all evaluated aspects of BMPC biocompatibility (adhesion, growth, and osteoblastic and chondrocytic differentiation). In addition, there was no detectable effect of collagen or vanadium-loaded scaffolds on macrophage viability or cytotoxicity. Based on these findings, we have developed a new ordered collagen scaffold loaded with VOAsc that shows potential for osteochondral tissue engineering.","palabras_clave":"escaffolds colageno|biocompatiblidad|osteoblastos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4550,"idioma_id":4,"titulo":"Exposure to low UVA doses increases KatA and KatB catalase activities and confers cross-protection against subsequent oxidative injuries in Pseudomonas aeruginosa","resumen":"Solar UVA radiation is one of the main environmental stress factors for Pseudomonas aeruginosa. Exposure to high UVA doses produces lethal effects by the action of the reactive oxygen species (ROS) it generates. P. aeruginosa has several enzymes, including KatA and KatB catalases, which provide detoxification of ROS. We have previously demonstrated that KatA is essential in defending P. aeruginosa against high UVA doses. In order to analyse the mechanisms involved in the adaptation of this micro-organism to UVA, we investigated the effect of exposure to low UVA doses on KatA and KatB activities, and the physiological consequences. Exposure to UVA induced total catalase activity; assays with non-denaturing polyacrylamide gels showed that both KatA and KatB activities were increased by radiation. This regulation occurred at the transcriptional level and depended, at least partly, on the increase in H2O2 levels. We demonstrated that exposure to low UVA produced a protective effect against subsequent lethal doses of UVA, sodium hypochlorite and H2O2. Protection against lethal UVA depends on katA, whilst protection against sodium hypochlorite depends on katB, demonstrating that different mechanisms are involved in the defence against these oxidative agents, although both genes can be involved in the global cellular response. Conversely, protection against lethal doses of H2O2 could depend on induction of both genes and\/or (an)other defensive factor(s). A better understanding of the adaptive response of P. aeruginosa to UVA is relevant from an ecological standpoint and for improving disinfection strategies that employ UVA or solar irradiation.","palabras_clave":"UV-A|Pseudomonas |Catalase KAT B |cross-protection"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4551,"idioma_id":5,"titulo":"Alfabetización científica en el ámbito preescolar: primeros conocimientos como herramientas para la promoción de la salud y la prevención de la Enfermedad de Chagas","resumen":"En el presente trabajo se describe una experiencia educativa realizada en cuatro Jardines preescolares(Kindergarden) de un área rural de la provincia de La Rioja (Argentina). Esta zona es endémica para laenfermedad de Chagas, considerada uno de los principales problemas de salud pública de Latinoamérica.La experiencia involucró a un total de 198 alumnos de nivel inicial, de entre de 3 y 5 años de edad.Los niños, por medio de juegos y actividades recreativas, recibieron los primeros conocimientos científicosrelacionados a la forma de transmisión de esta grave enfermedad y pautas de cómo prevenirla. Almismo tiempo, se inculcaron contenidos procedimentales propios del trabajo en ciencia como el manejode lupas binoculares, la comparación, la elaboración de conclusiones en base a indagaciones guiadas.Los contenidos conceptuales consistieron en conocer e identificar a los insectos que transmiten el parásitoTrypanosoma cruzi, el cual es causante de la enfermedad de Chagas. Utilizando prácticas lúdicasse pretende dar un enfoque innovador para la apropiación de pautas de conducta que favorezcan la promociónde la salud y, al mismo tiempo, conseguir el acercamiento de los niños a la ciencia desde el nivelinicial.","palabras_clave":"EDUCACIÓN INICIAL|EDUCACIÓN PARA LA SALUD|ALFABETIZACIÓN CIENTIFICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4552,"idioma_id":4,"titulo":"Calix[n]arenes: active organocatalysts for the synthesis of densely functionalized piperidines by one-pot multicomponent procedure","resumen":"An efficient, suitable and high yielding method has been developed for the synthesis of different densely functionalized piperidine derivatives via pseudo-five component, one-pot domino reaction through a combination of b-ketoesters, aromatic aldehydes, and various amines using p-sulfonic acid calix[n]arenes as catalysts. The reaction was carried out in refluxing methanol, affording very good yields of the expected piperidine. Atomic economy, environmentally benign procedure, reuse of catalysts, and shortreaction time are some of the important features of this protocol.","palabras_clave":"Densely functionalized piperidines|p-Sulfonic acid calix[n]arenes|Heterocyclic|Multicomponent reactions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4553,"idioma_id":4,"titulo":"A very simple solvent-free method for the synthesis of 2- arylchromones using KHSO4 as a recyclable catalyst","resumen":"An efficient and solvent-free procedure for the synthesis of flavones using KHSO4 as a recyclable catalyst is described. The methodology represents an environmentally friendly process in comprehensive consideration compared with other catalytic systems listed in publications. This method provides a clean, simple, solvent-free reaction and useful alternatives to prepare flavones and chromones. The use of KHSO4 catalyst provides excellent yields, also leading to an easy separation and recovery of the catalysts, which allows both low environmental impact and low cost. Other green advantages of the method are the low formation of wastes and the replacement of corrosive, soluble mineral acid.","palabras_clave":"Flavones|Chromones|Solvent-free reaction|Catalytic procedure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4554,"idioma_id":4,"titulo":"p-Sulfonic acid calix[4]arene-functionalized alkylbridged organosilica in esterification reactions","resumen":"Two new p-sulfonic acid calix[4]arene- and p-sulfonic acid calix[6]arene-functionalizedorganosilica have been synthesized using the sol-gel method and applied as heterogeneouscatalysts in esterification reactions. The catalytic performance was evaluated using theesterification of carboxylic acids with ethanol, and good catalytic activity (i.e., 55-88%) wasobserved under the optimum reaction conditions. This study reports the first promisingexample of successful employment of calix[n]arenes as a heterogeneous catalyst forcatalytic esterification. The catalyst was easily separated by filtration and reused five timeswithout any significant loss of activity.","palabras_clave":"p-Sulfonic acid|p-Sulfonic acid calix[4]arene-functionalized alkylbridged organosilica|Esterification reactions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4555,"idioma_id":4,"titulo":"TPRV-1 expression in human preeclamptic placenta","resumen":"Preeclampsia is a multisystem disorder unique to human pregnancy, characterized by abnormal placentation. Although its causes remain unclear, it is known that the expression of several transporters is altered. Transient receptor potential vanilloid 1 (TRPV-1) is a non-selective cation channel, present in human placenta. Here, we evaluated the expression of TRPV-1 in preeclamptic placentas. We observed a deregulation in TRPV-1 expression in these placentas which may explain the impaired Ca2+ homeostasis found in preeclampsia.","palabras_clave":"trophoblast|TRPV-1|preeclampsia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4556,"idioma_id":4,"titulo":"Increased Expression of Aquaporin 9 in Trophoblast From Gestational Diabetic Patients.","resumen":"Vilariño-García T, Pérez-Pérez A, Dietrich V, Fernández-Sánchez M, Guadix P, Dueñas JL, Varone CL, Damiano AE, Sánchez-Margalet VGestational diabetes mellitus is the most frequent pathophysiological alteration in pregnancy, increasing the incidence of complications in both mother and fetus. The macrosomia that occurs in these fetuses may be related with some changes in nutrient transport mechanism in placenta. The presence of aquaporin 9, an aquaglyceroporin, has previously been demonstrated in placenta. We raised the question whether aquaporin 9 expression may be upregulated in placenta from gestational diabetes, thus providing a faster transport of glycerol and water through placenta. We studied 21 placentas (13 controls and 8 gestational diabetes) from cesarean delivery at term. The expression of aquaporin 9 was analyzed by quantitative PCR, immunoblot, and immunohistochemistry. The median values from quantitative PCR were compared by nonparametric tests for independent samples (Mann-Whitney U-test). We have found that trophoblast from gestational diabetes express higher amount of aquaporin 9, which was found statistically significant (p<0.05). The increase in aquaporin 9 expression was confirmed by immunoblot, and localization in the syncytiotrophoblast was checked by immunohistochemistry. The increase in aquaporin 9 expression in placenta from gestational diabetes may contribute to the higher transport rate in this pathology of pregnancy.","palabras_clave":"AQP9|trophoblast|gestational diabetes|placenta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4557,"idioma_id":4,"titulo":"Biotemplated synthesis of mesoporous silica for doxycycline removal. Effect of pH, temperature, ionic strength and Ca2+ concentration on the adsorption behaviour","resumen":"Mesoporous silica (SiO2) materials were synthesized in acidic media, using tetraethyl orthosilicate as silica precursor and the water-soluble biopolymer hydroethyl starch (HES) as template, in order to evaluate the effect of temperature synthesis on the morphology and pore structure of synthesizedmaterials. The results show that the mentioned parameters can be tuned by changing the temperature, i.e., from slices with worm-like mesopores of around 5 nm to microporous lamellas when the temperature synthesis increases from 60ºC to 100ºC, respectively. The changes are attributed to a (partial) degradation of the template that hinders the gel formation and disturbs its interaction with the silica species through H-bonds formations and\/or electrostatic interactions. The synthesized solids were evaluated as adsorbent of the antibiotic doxycycline (DC) at several values of pH, temperature, ionic strength and Ca2+ concentration. The adsorption of DC strongly increases as pH and ionic strength decrease due to electrostatic attractions and H-bond formations between the functional groups of the antibiotic and the silica active sites. The presence of calcium ions strongly increases the adsorption of DC at pH > 4.4 due to the formation of ternary DC-Ca2+-SiO2 complexes by calcium-bridging. However,Ca2+ concentrations higher than 103 M cause the DC precipitation at pH 7 or higher. The analysis of  thermodynamic parameters suggests that the adsorption of DC on SiO2 is exothermic and spontaneous in nature. The effect of the tetracycline structure on the adsorption capacity of the synthesized material was also evaluated and discussed.","palabras_clave":"MESOPOROUS SILICA|HYDROXYETHYL STARCH|SOLID-WATER INTERFACE|DOXYCYCLINE ADSORPTION|CATION BRIDGING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4558,"idioma_id":4,"titulo":"Magnetic mesoporous silica for water remediation: Synthesis, characterization and application as adsorbent of molecules and ions of environmental concern","resumen":"Magnetic mesoporous silicas (NSMSiO2) were synthesized by coating nano-sized magnetite (NSM) particles with mesoporous silica shells. The effects of the NSM loading on the morphology, charge development and adsorption properties of the synthesized composites were investigated. Both the shape and the size of aggregates strongly changed as NSM\/SiO2 molar ratio increased, i.e., from plates to spherical-like particles, in agreement with reduction on the silica shell thickness. The shell acted as a protective agent that minimized alteration of the magnetite structure and avoided magnetite dissolution in acidic media. The composites have a significant adsorption capacity towards a humic acid and the cationic dye malachite green, showing that they can adsorb both, anionic and cationic species. HA seems to enter the pores and bind the NSM cores, whereas malachite green binds mainly the silica shell. The effect of Ca2+ concentration on the adsorption capacity of the synthesized composite was also evaluated and discussed. The magnetic property of the materials facilitates the recuperation of the adsorbent from aqueous environments.","palabras_clave":"MAGNETIC MESOPOROUS SILICA|CORE-SHELL PARTICLES|SOLID-WATER INTERFACE|POLLUTANT ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4559,"idioma_id":4,"titulo":"The CA domain of the respiratory complex I is required for  normal embryogenesis in  Arabidopsis thaliana","resumen":"The NADH-ubiquinone oxidoreductase [complex I (CI), EC 1.6.5.3] of the mitochondrial respiratory chain is the principal entry point of electrons, and vital in maintaining metabolism and the redox balance. In a variety of eukaryotic organisms, except animal and fungi (Opisthokonta), it contains an extra domain composed of putative gamma carbonic anhydrases subunits, named the CA domain, which was proposed to be essential for complex I assembly. There are two kinds of carbonic anhydrase subunits: CAs (of which there are three) and carbonic anhydrase-like proteins (CALs) (of which there are two). In plants, the CA domain has been linked to photorespiration. In this work, we report that Arabidopsis mutant plants affected in two specific CA subunits show a lethal phenotype. Double homozygous knockouts ca1ca2 embryos show a significant developmental delay compared to the non-homozygous embryos, which show a wild-type (WT) phenotype in the same silique. Mutant embryos show impaired mitochondrial membrane potential and mitochondrial reactive oxygen species (ROS) accumulation. The characteristic embryo greening does not take place and fewer but larger oil bodies are present. Although seeds look dark brown and wrinkled, they are able to germinate 12 d later than WT seeds. However, they die immediately, most likely due to oxidative stress.Since the CA domain is required for complex I biogenesis, it is predicted that in ca1ca2 mutants no complex I could be formed, triggering the lethal phenotype. The in vivo composition of a functional CA domain is proposed.","palabras_clave":"EMBRYOGENESIS|COMPLEX I|GAMMA CARBONIC ANHYDRASES|ARABIDOPSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4560,"idioma_id":4,"titulo":"Atmospheric sink of methyl chlorodifluoroacetate and ethyl chlorodifluoroacetate: temperature dependent rate coefficients, product distribution of their reactions with Cl atoms and CF2ClC(O)OH formation","resumen":"Rate coefficients in function of temperature have been measured for the first time for the gas-phase reactions of chlorine atoms with methyl chlorodifluoracetate (k1) and ethyl chlorodifluoroacetate (k2) using the relative rate technique. The experiments were carried out in a 1080 L photoreactor over the temperature range 287?313 K at a total pressure of 1000 ± 10 mbar of synthetic air using in situ FTIR spectroscopy to monitor reactants and products. The following Arrhenius expressions were obtained: k(MCDFA+Cl) = (9.6 ± 5.1) x 10-12 exp [-(1363 ± 79)\/T] and k(ECDFA+Cl) = (64.4 ± 29.7) x 10-12 exp [-(1110 ± 68)\/T]. The kinetic results are compared with previous experimental and theoretical studies.In addition, a product study of the reactions of Cl with methyl chlorodifluoracetate and ethyl chlorodifluoroacetate is reported. The results indicate that in the absence of NOx the main fate of the alkoxy radicals formed after H-atom abstraction by Cl from the ?CH3 group in methyl chlorodifluoroacetate is reaction of the radical with O2 to form the mixed anhydride CF2ClC(O)OC(O)H. In the case of ethyl chlorodifluoroacetate the main fate of the alkoxy fromed via H-atom abstraction by Cl from the ?CH2- entity in the ethyl group is an &#945;-ester rearrangement to produce chlorodifluoroacetic acid and .the corresponding radical.The yields of chlorofluoracetic acid (CF2ClC(O)OH) obtained were as follows: (34 ± 5) % and (86 ± 8) %  for the reactions of Cl with CF2ClC(O)OCH3 and CF2ClC(O)OCH2CH3, respectively. The measured yields are rationalized in terms of mechanisms consisting of competitive reaction channels for the formed in the oxidation, i.e. reaction with O2, &#61537;-ester rearrangement and a decomposition pathway.Atmospheric implications are discussed according to the rate coefficients obtained in function of the temperature and the altitude, and regarding to the formation of chlorofluorocarboxylic acid.","palabras_clave":"CHLOROFLUOROACETATES|TEMPERATURE DEPENDENCES|PRODUCTS STUDIES|CHLORODIFLUOROACETIC ACID FORMATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4561,"idioma_id":4,"titulo":"Kinetics of OH- and Cl-initiated oxidation of CH 2 CHC(O)O(CH 2 ) 2 CH 3 and CH 2 CHCH 2 C(O)O(CH 2 ) 2 CH 3 and fate of the alkoxy radicals formed","resumen":"Rate coefficients of the reactions of OH and Cl radicals with vinyl and allyl butyrate were determined forthe first time at 298 K and 1 atm using the relative method to be (in cm3 molecule1 s1): k1(OH + CH2=CHC(O)O(CH2)2CH3) = (2.61 ± 0.31)  1011, k2(Cl + CH2=CHC(O)O(CH2)2CH3) = (2.48 ± 0.89)  1010,k3(OH + CH2=CHCH2C(O)O(CH2)2CH3) = (2.89 ± 0.31)  1011, and k4(Cl + CH2=CHCH2C(O)O(CH2)2CH3) =(2.25 ± 0.96)  1010. Reactivity trends and atmospheric lifetimes of esters are presented. Additionally, aproduct study shown butyric acid and polifunctional products for the reactions of vinyl and allyl butyrate,respectively and general mechanism is proposed.","palabras_clave":"VINYL BUTYRATE|ALLYL BUTYRATE|TEFLON BAG|PRODUCT IDENTIFICATION ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4562,"idioma_id":4,"titulo":"Arrhenius parameters for the OH-initiated degradation of methyl crotonate, methyl-3,3-dimethyl acrylate, (E)-ethyl tiglate and methyl-3-butenoate over the temperature range of 288-314 K","resumen":"The relative-rate technique has been employed to obtain rates coefficients for the reactions between the OH radical and four unsaturated esters (methyl crotonate (k1), methyl-3,3-dimethyl acrylate (k2), (E)-ethyl tiglate (k3) and methyl-3-butenoate (k4)) between 288-314 K in 760 Torr of synthetic air. The experiments were performed in a multiple-pass environmental chamber with in situ FTIR detection of the esters and reference compounds. The rate coefficients for the reactions displayed a negative dependence with temperature and low pre-exponential factors. The following Arrhenius expressions (in units of cm3 molecule-1 s-1) were obtained: k1 = (3.39 ± 0.78) × 10-12 exp[(750 ± 159) &#8260; T], k2 = (2.95 ± 0.63) × 10-12 exp[(838 ± 182) &#8260; T] , k3 = (1.49 ± 0.56) × 10-12 exp[(522 ± 114) &#8260; T] and k4 = (1.90 ± 1.25) × 10-12 exp[(834 ± 185) &#8260; T]. The kinetic results are in agreement with a mechanism involving mainly OH radical addition to the double with the reversible formation of an OH-adduct. Atmospheric implications are discussed with particular reference to the rate coefficients obtained as a function of the temperature.","palabras_clave":"Arrhenius Parameters|Simulation Chambers|OH radicals|Unsaturated Esters"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4563,"idioma_id":4,"titulo":"- Efficacy and productive performance of moxidectin in feedlot calves infected with nematodes resistant to ivermectin","resumen":"Anthelmintic resistance (AR) of gastrointestinal nematodes to macrocyclic lactones is an increasinglycommon worldwide phenomenon limiting cattle production. This has motivated the search foralternatives, such as new active compounds, added drug synergisms, different doses, and alternate administrationroutes. The aim of this study was the assessment of moxidectin (MXD) performance in feedlotcalves with a history of AR to ivermectin (IVM). Crossbred female calves aged 6?7 months and weighing163 kg (SD = 34 kg) were divided into 3 groups of 35 animals each. They were assigned to the followingantiparasitic treatment groups: IVM group (0.2 mg\/kg IVM); MXD group (0.2 mg\/kg MXD), and ricobendazole+ levamisole (RBZ + LEV) group (7.5 mg\/kg RBZ + 8 mg\/kg LEV). On days 0, 26, and 47, fecal sampleswere taken and the weight of each animal was registered. Anthelmintic efficacy (by fecal egg count reduction),total weight gain (TWG) and average daily weight gain (AWG) were compared between the groups.A mixed SAS procedure was used for statistical analysis. Fecal egg count reduction 26 days post-treatment(PT) was calculated at 28% for the IVM group, 85% for the MXD group, and 99% for the RBZ + LEV group.AWGs (Standard Error) of 1.095 g (56), 1.264 g (49), and 1.340 g (52) were registered for the IVM, MXD,and RBZ + LEV groups, respectively (p < 0.05). Coprocultures revealed that MXD more effectively reducedHaemonchus spp. and Cooperia spp. egg counts than IVM. This resulted in higher AWGs and TWGs for thisgroup; similar results were seen for the RBZ + LEV group as well. In this study, animals treated with MXDgained about 160 more g\/day than animals treated with IVM. This represents a gain of 16 USD per animalover the 47 day trial.","palabras_clave":"Cattle |Macrocyclic lactones |Haemonchus |Cooperia |Average weight gain"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4564,"idioma_id":5,"titulo":"MÉTODOS INDIRECTOS PARA EVALUAR COGNICIONES IMPLÍCITAS HACIA EL ALCOHOL: UNA REVISIÓN CONCEPTUAL","resumen":"Desde su aparición en la década del 70 el Modelo del Doble Procesamiento (MDP) ha ganado una gran popularidad en diversos ámbitos de la psicología, principalmente en el ámbito de las adicciones. Según este modelo existen dos sistemas cognitivos: uno explícito, conciente y controlable; y otro implícito, automático y más ligado a la intuición y al afecto. El MDP se ha empleado para comprender los factores que subyacen a los comportamientos relacionados con el alcohol y en poco tiempo se produjo un crecimiento exponencial de las investigaciones que utilizan medidas implícitas para evaluar cogniciones relacionadas hacia el consumo de alcohol. Si bien estos métodos comparten algunas características básicas, difieren en aspectos importantes (evidencias de validez disponibles, condiciones de administración, etc). Por ello, este trabajo pretende ofrecer un panorama comprensivo de las distintas formas de evaluar la cognición implícita hacia el alcohol. Esperamos que esta revisión resulte de utilidad no solo para los investigadores, sino también para los profesionales que se ocupan de los problemas relacionados con el consumo de alcohol en el ámbito clínico.","palabras_clave":"ALCOHOL |COGNICIONES IMPLÍCITAS|MÉTODOS INDIRECTOS|MODELO DEL DOBLE PROCESAMIENTO|REVISIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4565,"idioma_id":5,"titulo":"Problemas por uso de alcohol en Estudiantes Secundarios y Universitarios","resumen":"El objetivo de este estudio fue caracterizar la presencia de problemas por uso de alcohol en estudiantes de secundaria y universitarios de Mar del Plata, Argentina. Se estimó la frecuencia según la edad, la probabilidad de presentar cada problema, y la prevalencia de búsqueda de ayuda por problemas por uso de alcohol. Se obtuvieron muestras probabilísticas de dos escuelas de educación secundaria (n = 305, 52% varones) y de la Universidad Nacional de Mar del Plata (n = 1427, 41% varones) entre los meses de abril-noviembre de 2014. Los problemas por uso de alcohol se estimaron con el cuestionario AUDIT-P, y la búsqueda de ayuda con una pregunta simple de formato dicotómico. En ambos grupos se hallaron altas prevalencias de problemas por uso de alcohol, en particular el descuido de actividades, los sentimientos de culpa y remordimiento, y el olvido de hechos ocurridos. A pesar de que los estudiantes universitarios en algunos casos duplicaron a los estudiantes de secundaria en la prevalencia de problemas por uso de alcohol, la prevalencia de búsqueda de ayuda fue del 2%. Se discute la repercusión de estos hallazgos en estrategias de prevención y tratamiento de problemas por uso de alcohol.","palabras_clave":"CONSECUENCIAS ADVERSAS|CONSUMO DE ALCOHOL|ESTUDIANTES DE SECUNDARIA|UNIVERSITARIOS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4566,"idioma_id":5,"titulo":"Investigación en intervención breve y consumo de alcohol durante la gestación: productividad e impacto","resumen":"Objetivo: Describir la producción científica de las Intervenciones Breves (IB) para disminuir el consumo de alcohol durante la gestación y cuantificarla mediante indicadores bibliométricos. Método: Se realizó un estudio de investigación secundaria con un diseño de revisión estructurada que consistió en búsquedas sistemáticas en las bases de datos Lilacs, PsycINFO, PubMed, Scielo y Scopus. Para el análisis se accedió al documento completo y se registraron características y procedimientos de aplicación de IB de los documentos seleccionados. Resultados: Se analizaron 45 artículos, la mayoría de los Estados Unidos. La primera publicación fue en 1999 registrándose un incremento progresivo hasta la actualidad. La revista con más publicaciones fue Obstetrics and Gynecology y el idioma predominante el inglés. El 52% de los artículos corresponden a trabajos empíricos, y el resto a trabajos de reflexión y revisiones que en su mayoría, promueven la abstinencia durante la gestación. En los trabajos experimentales se compararon dos grupos, intervención y control, y se registraron dos modalidades: vía web y cara a cara (71%). Para tamizar consumo de alcohol el T-ACE fue el instrumento más recomendado y utilizado, junto con preguntas de cantidad y frecuencia. Conclusiones: La necesidad de contar con estrategias efectivas para evitar el consumo de alcohol durante la gestación está siendo cada vez más reconocida. Se espera que en los próximos años el crecimiento del número de publicaciones impacte en nuestro contexto latinoamericano donde hay escasez de publicaciones, aparentemente nulas en habla hispana, y donde existe una relevante necesidad de evidencia sobre estrategias efectivas en esta población.","palabras_clave":"INTERVENCIONES BREVES|BEBIDAS ALCOHÓLICAS|PROMOCIÓN DE LA SALUD|EMBARAZO|INDICADORES BIBLIOMÉTRICOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4567,"idioma_id":4,"titulo":"Gender and Alcohol Use Disorders Diagnostic Criteria in Emergency Department Patients of Argentina","resumen":"Background: Alcohol consumption and its related consequences are not equal forwomen and men, although related studies do not frequently include gender analysis. Objective: Our aim was to characterize differences in endorsement of ICD-10 and DSM 5 alcohol use disorder (AUD) criteria by gender in an Argentinian emergency department population. Methods: A probability sample of patients (N = 923) from the largest emergency department in the city of Mar del Plata, Argentina (44% were females, aged 16 to 86, M (SD) = 37.31(15.20) was collected. Using a structured questionnaire, diagnostic criteria for alcohol use disorders, alcohol consumption, and socio-demographic variables were obtained. Bivariate and multivariate analyses were used to assess differences in the endorsement ofeach diagnostic criterion by gender. Results: Women were less likely to endorse each of the criteria for each of the diagnostic schemes. Even after controlling alcohol consumption,socio-demographic variables, severity of alcohol use disorders and adjusting for multiple comparisons females had a lower probability than males of endorsing withdrawal and impaired control. Conclusions: gender differences in the endorsement of diagnostic criteria for both the DSM 5 and ICD-10 were found. Some differences in endorsement but not all, might be partially explained by alcohol consumption patterns and socio-demographic factors,and same remained after controlling severity of the AUD. Results also suggest a differential functioning of DSM 5 and ICD-10 AUD criteria for women and men.","palabras_clave":"ALCOHOL|DIAGNOSTIC CRITERIA|DSM & ICD|GENDER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4568,"idioma_id":5,"titulo":"Registro de Información en Pacientes con Trastornos por uso de Alcohol en Mar del Plata","resumen":"Introducción: Los datos que se registran sobre el consumo de alcohol son importantes para el diagnóstico y pronóstico de trastornos por uso de alcohol. Este estudio descriptivo tuvo por objetivo caracterizar las referencias sobre consumo de alcohol y otras variables relacionadas en los registros hospitalarios de pacientes atendidos en una unidad especializada en alcoholismo de un hospital público de la ciudad de Mar del Plata, Argentina. Metodología: Se examinaron los registros de 60 admisiones entre febrero y noviembre de 2011, y se categorizaron extrayendo datos referidos al consumo de alcohol, fuente de la derivación, diagnóstico, tratamiento, internaciones y otras enfermedades, y aspectos del entorno familiar y socio-económico. Posteriormente se realizaron análisis descriptivos teniendo en cuenta el porcentaje de registros en los que se mencionaban estos aspectos. Resultados: Si bien el registro de datos referentes al consumo de alcohol fue alto, por lo general fue incompleto. La información registrada denotó que las condiciones bio-psico-sociales en las que se encuentran estos pacientes son poco favorables para un tratamiento exitoso. Conclusiones: Se discute la necesidad de sistematizar la información, por ejemplo a través de historias clínicas unificadas, junto con formas de prevenir la progresión de trastornos por uso de alcohol.","palabras_clave":"TRASTORNOS POR USO DE ALCOHOL|ATENCION HOSPITALARIA|REGISTROS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4569,"idioma_id":4,"titulo":"Historical records of mercury in southern latitudes over 1600 years: Lake Futalaufquen, Northern Patagonia","resumen":"Mercury is released to the environment from naturaland anthropogenic sources, and through atmospheric transport is distributedglobally. Lake Futalaufquen (42.8°S) is an oligotrophic lake located in LosAlerces National Park (Northern Patagonia), providing a remote and unpollutedstudy system. A lacustrine sedimentary sequence revealed 1600 years of Hgdeposition, identifying natural baselines and marked peaks not correlated withlong range atmospheric transport. Organic matter and catchment erosion were discardedas Hg drivers. Natural background, pre-1300 CE Hg concentrations, rangedbetween 27 and 47 ng g&#8722;1 (accumulation rates from 8 to 15 ìg m&#8722;2 y&#8722;1).From 1300 CE on, the Hg background profile did not follow the generallyincreasing Hg pattern observed in both Southern and Northern Hemisphere sincepre-industrial times. It was not until the last century that a 1.6-foldincrease is observed in the Hg accumulation rate, considered among the lowestincrements in southern South America. Noteworthy local\/regional sources of Hgfor this area, along with global transport, are forest fires and volcanicactivity. Between approx. 1340 and 1510 CE, sharp increase in Hg concentrationand accumulation rate (up to 204 ng g&#8722;1 and 51 ìg m&#8722;2 y&#8722;1,respectively) were clearly associated with extended fire episodes. Furthermore,high Hg peaks during the last 300 years were associated with volcanic eruptionsin northernmost Patagonia together with fairlyirregular fire episodes, caused by anthropogenic burning by settling population in the Andes.","palabras_clave":"Argentina|volcanism|atmospheric depositation|fires|global pollutant"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4570,"idioma_id":4,"titulo":"Elemental composition of Usnea sp lichen from Potter Peninsula, 25 de Mayo (King George) Island, Antarctica","resumen":"Several pollutants, which include metals, are present in the Antarctic atmosphere, snow, marine andterrestrial organisms. This work reports the elements incorporated by Usnea sp thalli in Potter Peninsula,25 de Mayo (King George) Island, South Shetlands, Antarctica. Geological origin was analyzed as possiblesources of elements. For this purpose, correlations were done using a geochemical tracer, principalcomponent analysis and enrichment factors were computed. Lithophile elements from particulate matterwere present in most of the sampling sites. Bromine, Se and Hg showed the highest enrichment factorssuggesting other sources than the particulate matter. Mercury values found in Usnea sp were in the samerange as those reported for Deception Island (South Shetlands) and remote areas from the PatagoniaAndes.","palabras_clave":"Pollution|Marine Airborne|South Shetlands|Biomonitor|Lichen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4571,"idioma_id":4,"titulo":"Bremsstrahlung enhancement in electron probe microanalysis for homogeneous samples using Monte Carlo simulation","resumen":"Fluorescence enhancement in samples irradiated in a scanning electron microscope or an electron microprobe should be appropriately assessed in order not to distort quantitative analyses. Several models have been proposed to take into account this effect and current quantification routines are based on them, many of which have been developed under the assumption that bremsstrahlung fluorescence correction is negligible when compared to characteristic enhancement; however, no concluding arguments have been provided in order to support this assumption. As detectors are unable to discriminate primary from secondary characteristic X-rays, Monte Carlo simulation of radiation transport becomes a determinant tool in the study of this fluorescence enhancement. In this work, bremsstrahlung fluorescence enhancement in electron probe microanalysis has been studied by using the interaction forcing routine offered by PENELOPE 2008 as a variance reduction alternative. The developed software allowed us to show that bremsstrahlung and characteristic fluorescence corrections are in fact comparable in the studied cases. As an extra result, the interaction forcing approach appears as a most efficientmethod, not only in the computation of the continuum enhancement but also for the assessment of the characteristic fluorescence correction.","palabras_clave":"EPMA|FLUORESCENCE ENHANCEMENT|ZAF CORRECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4572,"idioma_id":4,"titulo":"Development of camelid single chain antibodies against Shiga toxin type 2 (Stx2) with therapeutic potential against Hemolytic Uremic Syndrome (HUS)","resumen":"Shiga toxin (Stx)-producing Escherichia coli (STEC) infections are implicated in the development of thelife-threatening Hemolytic Uremic Syndrome (HUS). Despite the magnitude of the social and economicproblems caused by STEC infections, no licensed vaccine or effective therapy is presently availablefor human use. Single chain antibodies (VHH) produced by camelids exhibit several advantages incomparison with conventional antibodies, making them promising tools for diagnosis and therapy.In the present work, the properties of a recently developed immunogen, which induces high affinityand protective antibodies against Stx type 2 (Stx2), were exploited to develop VHHs with therapeuticpotential against HUS. We identified a family of VHHs against the B subunit of Stx2 (Stx2B) thatneutralize Stx2 in vitro at subnanomolar concentrations. One VHH was selected and was engineeredinto a trivalent molecule (two copies of anti-Stx2B VHH and one anti-seroalbumin VHH). The resultingmolecule presented extended in vivo half-life and high therapeutic activity, as demonstrated in threedifferent mouse models of Stx2-toxicity: a single i.v. lethal dose of Stx2, several i.v. incremental doses ofStx2 and intragastrical STEC infection. This simple antitoxin agent should offer new therapeutic optionsfor treating STEC infections to prevent or ameliorate HUS outcome.","palabras_clave":"SHIGA TOXIN|VHH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4573,"idioma_id":4,"titulo":"Induction of neutrophil extracellular traps in Shiga toxin-associated Hemolytic Uremic Syndrome","resumen":"Hemolytic uremic syndrome (HUS), a vascular disease characterized by hemolytic anemia, thrombocytopenia, and acute renal failure, is caused by enterohemorrhagic Shiga toxin (Stx)-producing bacteria, which mainly affect children. Besides Stx, the inflammatory response mediated by neutrophils (PMN) is essential to HUS evolution. PMN can release neutrophil extracellular traps (NET) composed of DNA, histones, and other proteins. Since NET are involved in infectious and inflammatory diseases, the aim of this work was to investigate the contribution of NET to HUS. Plasma from HUS patients contained increased levels of circulating free-DNA and nucleosomes in comparison to plasma from healthy children. Neutrophils from HUS patients exhibited a greater capacity to undergo spontaneous NETosis. NET activated human glomerular endothelial cells, stimulating secretion of the proinflammatory cytokines IL-6 and IL-8. Stx induced PMN activation as judged by its ability to trigger reactive oxygen species production, increase CD11b and CD66b expression, and induce NETosis in PMN from healthy donors. During HUS, NET can contribute to the inflammatory response and thrombosis in the microvasculature and thus to renal failure. Intervention strategies to inhibit inflammatory mechanisms mediated by PMN, such as NETosis, could have a potential therapeutic impact towards amelioration of the severity of HUS.","palabras_clave":"NETs|NEUTROPHILS|SHIGA TOXIN|HUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4574,"idioma_id":4,"titulo":"Heavy metal and trace elements in riparian vegetation and macrophytes associated with lacustrine systems in Northern Patagonia Andean Range","resumen":"Vegetation associated with lacustrine systems inNorthern Patagonia was studied for heavy metal and traceelement contents, regarding their elemental contribution tothese aquatic ecosystems. The research focused on native spe-cies and exotic vascular plantSalixspp. potential for absorb-ing heavy metals and trace elements. The native species stud-ied were riparianAmomyrtus luma, Austrocedrus chilensis,Chusquea culeou,Desfontainia fulgens,Escallonia rubra,Gaultheria mucronata,Lomatia hirsuta,Luma apiculata,Maytenus  boaria,Myrceugenia  exsucca,Nothofagusantarctica,Nothofagus dombeyi,Schinus patagonicus, andWeinmannia trichosperma, and macrophytesHydrocotylechamaemorus,Isöetes  chubutiana,Galiumsp.,Myriophyllum quitense,Nitellasp. (algae),Potamogetonlinguatus,Ranunculussp., andSchoenoplectus californicus.Fresh leaves were analyzed as well as leaves decomposingwithin the aquatic bodies, collected from lakes Futalaufquenand Rivadavia (Los Alerces National Park), and lakes Morenoand Nahuel Huapi (Nahuel Huapi National Park). The ele-ments studied were heavy metals Ag, As, Cd, Hg, and U,major elements Ca, K, and Fe, and trace elements Ba, Br,Co, Cr, Cs, Hf, Na, Rb, Se, Sr, and Zn. Geochemical tracersLa and Sm were also determined to evaluate contamination ofthe biological tissues by geological particulate (sediment, soil,dust) and to implement concentration corrections.","palabras_clave":"freshwater ecosystem|Patagonia native plant|Salix spp|Lake Futalaufquen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4575,"idioma_id":4,"titulo":"Optical Nanoparticle Sorting Elucidates  Synthesis of Plasmonic Nanotriangles","resumen":"We investigate the optical and morphological properties of gold nanoparticles grown by reducing a gold salt with Na2S. Lasers are tuned to the observed plasmon resonances, and the optical forces exerted on the nanoparticles are used to selectively print individual nanoparticles onto a substrate. This enables us to combine dark-field spectroscopy and scanning electron microscopy to compare the optical properties of single nanoparticles with their morphology. By arresting the synthesis at different times, we are able to investigate which type of nanoparticle is responsible for the respective resonances. We find that thin Au nanotriangles are the source of the observed near infrared (NIR) resonance. The initial lateral growth of these triangles causes the plasmon resonance to redshift into the NIR, whereas a subsequent thickening of the triangles and a concomitant truncation lead to a blueshift of the resonance. Furthermore, we find that the nanotriangles produced have extremely narrow line widths (187 ± 23 meV), show nearly isotropic scattering, and are stable for long periods of time. This shows their vast potential for applications such as in vivo imaging and bio(chemical) sensing. The method used here is generally applicable to other syntheses, and shows how complex nanostructures can be built up on substrates by selectively printing NPs of varying plasmonic resonances.","palabras_clave":"OPTICAL PRINTING|GOLD NANOPARTICLES|CANCER THERAPY|NANOSHELLS|PLASMON|NANOPARTICLE SORTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4576,"idioma_id":4,"titulo":"Can elite maize landraces help to improve forage yield and quality? A genetic analysis","resumen":"Broadening the genetic base of maize breeding program is a significant concern for plant breeders, since itrestricts the magnitude of genetic gain. Identification of promissory exotic elite maize (Zea mays L) germplasmwould be useful to increase the genetic variation within typically used heterotic groups and to improve the forageyield and quality. This study is aimed to assess the genetic potential of a group of elite maize landraces to improveforage yield and quality related traits and to broaden the genetic base of three temperate heterotic groups. Meanvalues and landrace general combining ability effects (GCAl) revealed that some landraces could be considered asa valuable genetic resource to broaden the current genetic base through introgression of forage yield and qualityfavorable alleles. When stover and ear dry matter yield were considered, ARZM17035 was the best landrace sinceit produced the best performing landrace × inbred line crosses that also were stable across environments. Additionally,this landrace showed high GCAl. Considering quality traits, ARZM07134 was the most promising sourceof favorable alleles. The use of B73 as tester in a recurrent selection scheme would be the most efficient strategy,since both mentioned landraces crossed to B73, showed the highest yield and quality values. Additionally, evaluatedtraits were mainly controlled by additive effects, so it is expected to obtain a positive response by selection.","palabras_clave":"Recursos genéticos|Mejoramiento vegetal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4577,"idioma_id":4,"titulo":"Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations","resumen":"AbstractThe aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarose-in plug bridge method. Video image sequences were processed applying Shannon´s entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarose-in plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility. © 2016 Elsevier B.V.","palabras_clave":"chemotaxis|bacteria|video processing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4578,"idioma_id":4,"titulo":"New records of late Holocene tephras from Lake Futalaufquen (42.8\u0001S), northern Patagonia","resumen":"In regions with limited knowledge of the historical volcanic record, like remote areas in the AndeanSouthern Volcanic Zone, the definition of reliable age-depth models for lake sequences represents avaluable tool for tephra layers dating. In Lake Futalaufquen (42.8S), Northern Patagonia, a short sedimentarysequence was extracted after the AD 2008 Chaiten eruption with the purpose to analyze therecords of volcanic eruptions at these poorly studied latitudes. The sequence was dated by 210Pb, 137Cs,and 14C techniques. Five tephras were identified for the last 1600 years, restricted to the last 5 centuries.Sedimentology, morphology, and geochemical properties allowed the characterization of the tephras andtheir correlation with tephras recently identified proximal to the sources, mainly from Chaiten andHuequi volcanoes, and Michinmahuida accessory cones, representing the first distal records reported ofthese tephras. Furthermore, tephras modeled ages obtained by the sequence age-depth model shrink theages for the volcanic events, like a potential cycle of activity from Michinmauida accessory cones duringAD 1530 ± 55, one eruption from Huequi volcano at AD 1695 ± 50, and a possible recent eruption fromChaiten at AD 1775 ± 40. Additionally, the work contributes to improve the regional volcanic recordsknowledge, basic for volcanic hazard assessment.","palabras_clave":"tephra|lake sediments|Southern volcanic zone|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4579,"idioma_id":4,"titulo":"Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa","resumen":"Glycosidases may play a role in sperm maturation during epididymal transit. In this work we describe the interaction of these enzymes with bull spermatozoa. We found that &#946;-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-&#946;-D-glucosaminidase and &#946;-glucuronidase with spermatozoa appeared to be stronger. On the other hand, &#945;-mannosidase and &#945;-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-&#946;-D-glucosaminidase, &#946;-galactosidase and &#946;-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent- mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent- mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-&#946;-D-glucosaminidase and &#946;-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent- mannose-6-phosphate receptor, while &#946;-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes.","palabras_clave":"GLICOSIDASES|MANOSE-6-PHOSPHATE RECEPTORS|BULL SPERMATOZOA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4580,"idioma_id":4,"titulo":"EOCENE BASILOSAURID WHALES FROM THE LA MESETA FORMATION, MARAMBIO (SEYMOUR) ISLAND, ANTARCTICA","resumen":"AbstractAbstract. Basal fully aquatic whales, the basilosaurids, are worldwide known from Bartonian?Priabonian localities, indicating that this group was widely distributed during the late middle Eocene. In the Northern Hemisphere, fossils of basilosaurids are abundant, while records in the Southern Hemisphere are scarce and, in some cases (i.e. Antarctica), doubtful. The presence of basilosaurids in Antarctica was, until now, uncertain because most of the records are based on from fragmentary materials that precludes an accurate assignment to known archaeocete taxa. Here we report the findings of mandibles, teeth, and innominate bone remains of a basilosaurid recovered from the La Meseta Formation (TELM 4 Lutetian?Bartonian and; TELM 7 Priabonian), in Marambio (Seymour) Island (James Ross Basin, Antarctic Peninsula). These findings confirm the presence of Basilosauridae in the marine realm of Antarctica, increasing our knowledge of the paleobiogeographic distribution of basilosaurids during the middle?late Eocene. In addition, one of these records is among the oldest occurrences of basilosaurids worldwide, indicating a rapid radiation and dispersal of this group since at least the early middle Eocene.","palabras_clave":"Cetacea,|Basilosauridae|Paleogene|Cheek teeth| Mandibles| Cetacea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4581,"idioma_id":4,"titulo":"The RNA Response to DNA Damage","resumen":"Multicellular organisms must ensure genome integrity to prevent accumulation of mutations, cell death, and cancer. The DNA damage response (DDR) is a complex network that senses, signals, and executes multiple programs including DNA repair, cell cycle arrest, senescence, and apoptosis. This entails regulation of a variety of cellular processes: DNA replication and transcription, RNA processing, mRNA translation and turnover, and post-translational modification, degradation, and relocalization of proteins.Accumulated evidence over the past decades has shown that RNAs and RNA metabolism are both regulators and regulated actors of the DDR. This review aims to present a comprehensive overview of the current knowledge on the many interactions between the DNA damage and RNA fields.","palabras_clave":"Alternative splicing|DNA damage"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4582,"idioma_id":5,"titulo":"Liderazgo, confianza y flexibilidad laboral como predictores de identificación organizacional. Un estudio con trabajadores argentinos","resumen":"Objetivo: examinar el impacto del liderazgo transformador, la confianza en el supervisor y la flexibilidad laboral sobre la identificación del trabajador con su organización (IDO). Método: se realizó un estudio empírico, cuantitativo y transversal, enmarcado en la teoría de la identidad social. Se trabajó con una muestra intencional de 257 empleados de empresas argentinas con regímenes de trabajo flexible. Los participantes completaron las escalas de Identificación organizacional, Liderazgo transformador, Confianza en el supervisor y Flexibilidad laboral. El modelo de mediación moderada postulado fue probado usando análisis de regresión múltiple y análisis de efectos condicionales. Resultados: la confianza en el supervisor medió las relaciones entre liderazgo transformador e IDO, sea por afinidad (&#946; = 0.29, z = 4.26, p < 0.001), sea por imitación (&#946; = 0.31, z = 4.45, p < 0.001). Tales relaciones fueron moderadas por la flexibilidad laboral, siendo más fuertes sobre la IDO por afinidad (&#946;= 0.35, p< 0.05, IC= 0.29-0.41). Conclusiones: los líderes transformadores influyen sobre la IDO de los trabajadores a través de la confianza que generan, en tanto que la flexibilidad laboral amplifica tales relaciones. Se indican las implicancias prácticas de estos hallazgos y se señalan las fortalezas y debilidades del trabajo realizado.","palabras_clave":"IDENTIFICACIÓN ORGANIZACIONAL|CONFIANZA|FLEXIBILIDAD LABORAL|LIDERAZGO TRANSFORMADOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4583,"idioma_id":4,"titulo":"Perceptions of organizational justice and ambivalent sexism: The moderating role of individualism-collectivism","resumen":"The objective of the present study was, on the one hand, to explore the relationships between perceptions of interpersonal\/informational justice and ambivalent sexism among Argentinean employees, and, on the other hand, to examine the possible role that collectivism and individualism play on such relationships. Participants (128 men and 120 women) completed a battery of recognized instruments to measure the variables of interest. Results showed that interpersonal and informational justice perceptions were negatively related to hostile sexism, and that, unexpectedly, informational justice perceptions were positively associated to benevolent sexism. Vertical collectivism and vertical individualism moderated the relationships between interpersonal justice perceptions and hostile sexism. Findings are discussed in light of their theoretical and practical implications. Suggestions for future research are provided.","palabras_clave":"Organizational Justice|Sexism|Individualism|Collectivism|Values"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4584,"idioma_id":4,"titulo":"Fertilization defects in sperm from Cysteine-Rich Secretory Protein 2 (CRISP2) knockout mice: implications for fertility disorders","resumen":"STUDY HYPOTHESIS:We hypothesize that fertility disorders in patients with aberrant expression of Cysteine-RIch Secretory Protein 2 (CRISP2) could be linked to the proposed functional role of this protein in fertilization.STUDY FINDING:Our in vivo and in vitro observations reveal that Crisp2-knockout mice exhibit significant defects in fertility-associated parameters under demanding conditions, as well as deficiencies in sperm fertilizing ability, hyperactivation development and intracellular Ca2+ regulation.WHAT IS KNOWN ALREADY:Testicular CRISP2 is present in mature sperm and has been proposed to participate in gamete fusion in both humans and rodents. Interestingly, evidence in humans shows that aberrant expression of CRISP2 is associated with male infertility.STUDY DESIGN, SAMPLES\/MATERIALS, METHODS:A mouse line carrying a deletion in the sixth exon of the Crisp2 gene was generated. The analyses of the reproductive phenotype of Crisp2-\/- adult males included the evaluation of their fertility before and after being subjected to unilateral vasectomy, in vivo fertilization rates obtained after mating with either estrus or superovulated females, in vitro sperm fertilizing ability and different sperm functional parameters associated with capacitation such as tyrosine phosphorylation (by western blot), acrosome reaction (by Coomassie Blue staining), hyperactivation (by computer-assisted sperm analysis) and intracellular Ca2+ levels (by flow cytometry).MAIN RESULTS AND THE ROLE OF CHANCE:Crisp2-\/- males presented normal fertility and in vivo fertilization rates when mated with estrus females. However, the mutant mice showed clear defects in those reproductive parameters compared with controls under more demanding conditions, i.e. when subjected to unilateral vasectomy to reduce the number of ejaculated sperm (n = 5; P&lt; 0.05), or when mated with hormone-treated females containing a high number of eggs in the ampulla (n &#8805; 5; P&lt; 0.01). In vitro fertilization studies revealed that Crisp2-\/- sperm exhibited deficiencies to penetrate the egg vestments (i.e. cumulus oophorus and zona pellucida) and to fuse with the egg (n &#8805; 6; P&lt; 0.01). Consistent with this, Crisp2-null sperm showed lower levels of hyperactivation (n = 7; P&lt; 0.05), a vigorous motility required for penetration of the egg coats, as well as a dysregulation in intracellular Ca2+ levels associated with capacitation (n = 5; P&lt; 0.001).LIMITATIONS, REASONS FOR CAUTION:The analysis of the possible mechanisms involved in fertility disorders in men with abnormal expression of CRISP2 was carried out in Crisp2 knockout mice due to the ethical and technical problems inherent to the use of human gametes for fertilization studies.WIDER IMPLICATIONS OF THE FINDINGS:Our findings in mice showing that Crisp2-\/- males exhibit fertility and fertilization defects under demanding conditions support fertilization defects in sperm as a mechanism underlying infertility in men with aberrant expression of CRISP2. Moreover, our observations in mice resemble the situation in humans where fertility disorders can or cannot be detected depending on the accumulation of own individual defects or the fertility status of the partner. Finally, the fact that reproductive defects in mice are masked by conventional mating highlights the need of using different experimental approaches to analyze male fertility.STUDY FUNDING AND COMPETING INTERESTS:This study was supported by the World Health Organization (H9\/TSA\/037), the National Research Council of Argentina (PIP 2009-290), the National Agency for Scientific and Technological Promotion of Argentina (PICT 2011, 2023) and the Rene Baron Foundation to P.S.C. and by the MEXT of Japan to M.I. The authors declare that there are no conflicts of interest.","palabras_clave":"CRISP|testes|fertility|sperm|motility|fertilization|hyperactivation|knockout mice"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4585,"idioma_id":4,"titulo":"Acrosome reaction as a preparation for gamete fusion","resumen":"Acrosome reaction is a universal requisite for sperm-egg fusion. However, differently from what has been observed in most animals, eutherian gamete fusion occurs through a specialized region from the acrosome, known as the equatorial segment (ES), that becomes fusogenic only after acrosome reaction is completed. This review focuses on molecular mechanisms involved in the acquisition of fusogenicity of the ES after the acrosome reaction. We describe a group of proteins that have been proposed to be involved in this process by modifying molecules already present in this region or by relocalizing to the ES after the acrosome reaction where they may be involved in gamete fusion.","palabras_clave":"acrosome reaction|proteins|equatorial segment|sperm-egg fusion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4586,"idioma_id":4,"titulo":"The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm","resumen":"Sperm capacitation is required for fertilization. At the molecular level, this process is associated with a fast activation of protein kinase A. Downstream of this event, capacitating conditions lead to an increase in tyrosine phosphorylation. The identity of the tyrosine kinase(s) mediating this process has not been conclusively demonstrated. Recent experiments using stallion and human sperm have suggested a role for PYK2 based on the use of small molecule inhibitors directed against this kinase. However, critical loss-of-function experiments have not been reported. Here, we used both pharmacological inhibitors and genetically modified mice models to investigate the identity of the tyrosine kinase(s) mediating the increase in tyrosine phosphorylation in mouse sperm. Similar to stallion and human, PF431396 blocks the capacitation-associated increase in tyrosine phosphorylation. Yet, sperm from Pyk2?\/? mice displayed normal increase in tyrosine phosphorylation, implying that PYK2 is not responsible for this phosphorylation process. Here we show that PF431396 can also inhibit FER, a tyrosine kinase known to be present in sperm. Sperm from mice targeted with a kinase inactivating mutation in Fer failed to undergo capacitation-associated increases in tyrosine phosphorylation. While these mice are fertile, their sperm displayed a reduced ability to fertilize metaphase-II arrested eggs in vitro.","palabras_clave":"FER|CAPACITATION|TYROSINE PHOSPHORYLATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4587,"idioma_id":4,"titulo":"Li4(NH2)3Cl amide-chloride: a new synthesis route, and hydrogen storage kinetic and thermodynamic properties","resumen":"Amide-halide compounds were identified as possible promoters of the dehydrogenation kinetics of the Li-N-H system. However, reversible hydrogen storage capacities and sorption kinetics of Li4(NH2)3Cl and Li3Mg0.5(NH2)3Cl have not been reported yet. In the present work, Li4(NH2)3Cl was synthesized using a new synthesis route that involves the pre-milling of a LiNH2-LiCl mixture. Attempts to synthesize Li3Mg0.5(NH2)3Cl by applying similar synthesis procedures using LiNH2 and 0.5MgCl2 were unsuccessful; instead, a mixture of Li4(NH2)3Cl-0.5Mg(NH2)2 was obtained. The hydrogen storage properties of the Li4(NH2)3Cl-3LiH and Li4(NH2)3Cl-0.5Mg(NH2)2-3LiH composites were evaluated between 200 °C and 300 °C. The onset of hydrogen release was reduced by 20 °C when Li4(NH2)3Cl-3LiH decomposed in the presence of Mg(NH2)2 (180 °C with respect to 200 °C) and its hydrogen desorption rate increased by 83%. However, no change in the dehydrogenation activation energy was observed for Li4(NH2)3Cl-3LiH decomposition due to minor amounts of Mg(NH2)2. The hydrogen storage capacity under cycling was reduced from about 3.0 wt% to 1.5 wt% at 300 °C, after rehydrogenation at 6.0 MPa. The formation of Li7(NH)3Cl was clearly identified in the dehydrogenated material. Unfortunately, the sloped plateau and the thermodynamic stability of Li4(NH2)3Cl-3LiH precludes its hydrogen storage applicability.","palabras_clave":"HYDROGEN STORAGE|AMIDES-HALIDE|MAGNESIUM|DESTABILIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4588,"idioma_id":4,"titulo":"COMPARATIVE STUDY OF THE PHYSICOCHEMICAL PROPERTIES AND PHOTOCATALYTIC ACTIVITY OF MODIFIED MCM-41 SYNTHESIZED BY DIRECT (ONE-STEP) INCORPORATION AND IMPREGNATION.","resumen":"MCM-41structures were synthesized and modified by direct (one-step) incorporation (DI)and wet impregnation (WI) methods. The solids were characterized by XRF, XRD, N2 adsorptionand UV-Vis DR, and catalytically evaluated in the Acid Orange 7 (AO7) degradation under visiblelight. It was possible to infer the significant influence of the presence, dispersion and location ofthe different Cr species on the photoactivity. Both synthesis methods allow the higher dispersionof the photoactive Cr species for the lower metal content, which enhances the efficiency for dyedegradation. Particularly, after the Ti loading in samples synthesized by DI, the notable AO7degradation increase was associated with the strong heterojunction between Ti and Cr6+ species,which are highly dispersed due to the DI method used.","palabras_clave":"IMPREGNATION|DIRECT INCORPORATION|METALIC SPECIES|PHOTOCATALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4589,"idioma_id":4,"titulo":"Neighborhood and family perceived environments associated with children's physical activity and body mass index","resumen":"Objective. To investigate the association of neighborhood and family perceived environments, use of and distance to public open spaces with leisure-time physical activity (LTPA) and body mass index (BMI) in Argentinean school-aged children.Methods. School-based, cross-sectional study with 1777 children (9 to 11 years) and their parents, in Cordoba city during 2011. Children were asked about LTPA and family perceived environment. Parents were asked about neighborhood perceived environment, children´s use of public open spaces and distance. Weight and height were measured for BMI. We modeled children´s LTPA and BMI z-score with structural equation models with latent variables for built, social and safety neighborhood environments.Results. Parents´ perceived neighborhood environment was not related with children´s LTPA and BMI. Children´s perceived autonomy and family environment were positively associated with LTPA. Use of unstructured open spaces and, indirectly, the distance to these, was associated with LTPA among girls. Greater distance to parks reduced their use by children.Conclusions. Policies to increase children´s LTPA should include access to better public open spaces, increasing options for activity. A family approach should be incorporated, reinforcing its role for healthy development.","palabras_clave":"Built environment|Physical activity|children|body mass index"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4590,"idioma_id":4,"titulo":"IN VIVO ASSESSMENT OF  CLOSANTEL OVICIDAL ACTIVITY IN FASCIOLA HEPATICA EGGS","resumen":"The trematode Fasciola hepatica is a cosmopolitan parasite which causes considerable loss in sheep and cattle production systems all over the world. Chemotherapy is the main tool available to control of the liver flukes. The anthelmintic Closantel, is effective against a wide range of hepatic and intestinal trematodes in a variety of animals. It highly effective against F. hepatica with both an in vivo e in vitro efficacy against mature and immature stages, does not exhibit ovicidal activity in vitro. The ovicidal effects in vivo at present are undetermined. In the present work, modified egg hatch assay (EHA) was used to evaluate the in vivo ovicidal activity of Closantel on fluke eggs. For this purpose the exposition at the anthelmintic Closantel was at the host. At 12, 24 and 36 h post-treatment he eggs collected of the gall bladder was observed and immediately incubated without added drug at the assay. Regarding the morphometric analysis of the eggs, between experimental and control groups there were statistically  significant differences regarding width and shape yet no significant differences were found in length and size. Eggs exposed to the effect of closantel tend to be more elliptical. Significant differences were found in the hatching of eggs between 36 hs post treatment (32, 5%) vs. aproximately 85% in control,12h and 24 h post treatment. This work confirms Closantel affect in vivo the normal development of the eggs. As one of the first effects, this drug affects the performance of the trematode´s reproductive physiology. These results have an impact on the interpretation of the Faecal Egg Counts in ef&#64257;cacy studies and may be useful to further understand the mechanisms underlying the drug activity in target helminthes parasites ","palabras_clave":"FASCIOLA|CLOSANTEL|OVICIDAL ACTIVITY|EGGS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4591,"idioma_id":4,"titulo":"Evolution of the gate current in 32 nm MOSFETs under irradiation","resumen":"Radiation induced currents on single 32 nm MOSFET transistors have been studied using consecutiveruns of 16O at 25 MeV. The main feature is the generation of current peaks ? in the gate and channel currents? due to the collection of the electro?hole pairs generated by the incident radiation runs. It has beenobserved that the incident ions cause damage in the dielectric layer and in the substrate affecting the collectionof carriers, and hence the radiation-induced current peaks. It has been find out a decrease of thecurrent peak due to the increase of the series resistance by non-ionizing energy loss in the semiconductorsubstrate, and an increase of the leakage current due to defects in the gate oxide by ionizing energy loss.For low levels of damage in the gate oxide, the main feature is the shift of the VTH. Hot carriers heatedby the incident radiation in the depletion region and injected in the gate oxide cause the change of theVTH due to electron or hole trapping for n- or p-channel respectively. The overall results illustrate thatthese effects must be taken into consideration for an accurate reliability projection.","palabras_clave":"gate-current|MOSFET|radiation|current_peaks-induced"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4592,"idioma_id":5,"titulo":"La economía argentina y su conflicto distributivo estructural (1930-2015)","resumen":"En este trabajo, brindamos una interpretación del desempeño económico argentino  entre 1930 y 2015, en la que el conflicto distributivo juega un papel protagónico. Nuestro argumento sostiene que el conflicto surge de la inconsistencia entre las aspiraciones económicas arraigadas en la sociedad y las posibilidades productivas de la economía. Presentamos dicha inconsistencia de un modo preciso: como una divergencia entre dos niveles de equilibrio del tipo de cambio real. El tipo de cambio real de equilibrio macroeconómico es aquel que permite a la economía mantener simultáneamente el pleno empleo y un balance de pagos sostenible. El tipo de cambio real de equilibrio social es aquel que emerge cuando los trabajadores plenamente ocupados alcanzan el salario real al que aspiran. Estos niveles de tipo de cambio real pueden no coincidir. Existe un conflicto distributivo estructural cuando el tipo de cambio real de equilibrio macroeconómico es significativamente mayor al de equilibrio social. Desarrollamos un modelo formal que representa de modo estilizado la estructura de la economía argentina y que permite brindar una definición precisa de los dos niveles de equilibrio del tipo de cambio real y del conflicto estructural. Luego utilizamos el modelo para caracterizar sintéticamente la performance y política económica en el período 1930-2015.","palabras_clave":"Argentina|tipo de cambio real|salario real|conflicto"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4593,"idioma_id":4,"titulo":"Comparison between PCR and larvae visualization methods for diagnosis of Strongyloides stercoralis out of endemic area: A proposed algorithm","resumen":"Underdiagnosis of chronic infection with the nematode Strongyloides stercoralis may lead to severe dis-ease in the immunosuppressed. Thus, we have set-up a specific and highly sensitive molecular diagnosisin stool samples. Here, we compared the accuracy of our polymerase chain reaction (PCR)-based methodwith that of conventional diagnostic methods for chronic infection. We also analyzed clinical and epi-demiological predictors of infection to propose an algorithm for the diagnosis of strongyloidiasis usefulfor the clinician. Molecular and gold standard methods were performed to evaluate a cohort of 237 indi-viduals recruited in Buenos Aires, Argentina. Subjects were assigned according to their immunologicalstatus, eosinophilia and\/or history of residence in endemic areas. Diagnosis of strongyloidiasis by PCR onthe first stool sample was achieved in 71\/237 (29.9%) individuals whereas only 35\/237(27.4%) were pos-itive by conventional methods, requiring up to four serial stool samples at weekly intervals. Eosinophiliaand history of residence in endemic areas have been revealed as independent factors as they increase thelikelihood of detecting the parasite according to our study population. Our results underscore the useful-ness of robust molecular tools aimed to diagnose chronic S. stercoralis infection. Evidence also highlightsthe need to survey patients with eosinophilia even when history of an endemic area is absent.© 2016 Elsevier B.V. All rights reserved","palabras_clave":"STONGYLOIDES STERCORALIS|DIAGNOSTICO|EOSINOFILIA|INMUNOCOMPROMISO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4594,"idioma_id":4,"titulo":"First Genome Sequence of Leptospira interrogans Serovar Pomona, Isolated from a Bovine Abortion","resumen":"Leptospirosis is a widespread zoonosis and a re-emergent disease of global distribution with major relevance in veterinary production. Here, we report the whole-genome sequence of Leptospira interrogans serovar Pomona strain AKRFB, isolated from a bovine abortion during a leptospirosis outbreak in Argentina.","palabras_clave":"LEPTOSPIRA INTERROGANS|GENOMICA|SEROVAR POMONA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4595,"idioma_id":4,"titulo":"Carbohydrate production by microbial mats communities in tidal flat from Bah&#305;´a Blanca Estuary (Argentina)","resumen":"The seasonal and spatial distribution of colloidal and capsular extracellular carbohydrates was assessed in three sites in the supratidal flat from Rosales Port (middle zone of Bahía Blanca Estuary, Argentina) where microbial mats were developed. Extracellular carbohydrates, Chlorophyll a, phaeopigments, organic matter and moisture content were determined in surface (0?5 mm depth) and subsurface (5?10 mm depth) sediment layers. No differences in pH, Eh and temperature were observed among sites but seasonal differences were found in Eh and temperature. Organic matter content was statistically different among sites being higher in ST3, which has registered fine sediments. In turn, ST3 differs significantly from ST1 and ST2 in the Chlorophyll a concentrations in the subsurface layer and in colloidal and capsular carbohydrates content. In the three study sites, the extracellular carbohydrates concentration was higher in surface sediment layer than in subsurface layer. In addition, seasonal differences were found for all sites between surface and subsurface sediment layers. Maximum extracellular carbohydrates concentration was registered when the Chlorophyll a concentration was high. The relationship among the physicochemical parameters evaluated, grain size, Chlorophyll a and extracellular carbohydrates concentration allows defining two different areas in the Rosales Port despite the proximity.","palabras_clave":"Collodal Carbohydrates|capsular cabohydrates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4596,"idioma_id":4,"titulo":"B cells inhibits the antitumor immunity against an established murine fibrosarcoma","resumen":"Despite B cell classicrole in favouring immune response, an inhibitory role for B lymphocytes intumor immunity has been recently turned into focus. In the mouse fibrosarcomaMCC, the loss of immunogenicity and the establishment of tolerance areparalleled by systemic immune suppression and the appearance of IL-10-producing B cells in the tumor- draining lymph nodes. The aim of this paper wasto study the role of B cells in the immune evasion and tolerance exhibited byMCC by means of depleting these cells in tumor bearing mice. We found that Bcell depletion, once the tumor is established resulted in lower tumor growthand delayed onset of tolerance. Additionally, B cell absence exacerbated T celldependent- tumor rejection, reduced the regulatory T cell population andincreased cytotoxic CD8+ T cells both in the lymph node and tumor tissue. More,T cell dependent- tumor rejection was increased in B cell- absence. Contrarilyto these signs of immune activation, B cell depletion performed before tumorimplantation resulted in enhanced tumor progression[C1]&nbsp; suggesting theinhibition of the immune antitumor response. In vitro analysis showed a directeffect of B cells upon T cell proliferation partially mediated by IL-10 secretion.These results suggest that B cells contribute to the immunological tolerance ofMCC tumor and that B cell depletion would unmask a pre- existing antitumorresponse. The present findings draw attention to the convenience of modulatingB cells in the development of future and more effective immunotherapies againstcancer.&nbsp;","palabras_clave":"MURINE FIBROSARCOMA|CANCER|B CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4597,"idioma_id":4,"titulo":"Deletion of A44L, A46R and C12L Vaccinia Virus Genes from the MVA Genome Improved the Vector Immunogenicity by Modifying the Innate Immune Response Generating Enhanced and Optimized Specific T-Cell Responses","resumen":"MVA is an attenuated vector that still retains immunomodulatory genes. We havepreviously reported its optimization after deleting the C12L gene, coding for the IL-18 binding-protein.Here, we analyzed the immunogenicity of MVA vectors harboring the simultaneous deletion of A44L, related to steroid synthesis and A46R, a TLR-signaling inhibitor (MVADA44L-A46R); or also including a deletion of C12L (MVADC12L\/DA44L-A46R). The absence of biological activities of the deleted genes in the MVA vectors was demonstrated. Adaptive T-cell responses against VACV epitopes, evaluated in spleen and draining lymph-nodes of C57Bl\/6 mice at acute\/memoryphases, were of higher magnitude in those animals that received deleted MVAs compared to MVAwt. MVADC12L\/DA44L-A46R generated cellular specific memory responses of higher quality characterized by bifunctionality (CD107a\/b+\/IFN- +) and proliferation capacity. Deletion of selected genes from MVA generated innate immune responses with higher levels of determiningcytokines related to T-cell response generation, such as IL-12, IFN- , as well as IL-1 and IFN-.This study describes for the first time that simultaneous deletion of the A44L, A46R and C12L genes from MVA improved its immunogenicity by enhancing the host adaptive and innate immune responses, suggesting that this approach comprises an appropriate strategy to increase the MVA vaccine potential.","palabras_clave":"MVA|VACCINE|T-CELL RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4598,"idioma_id":4,"titulo":"Molecular Magnetism In Closo-Azadodecaborane Supericosahedrons","resumen":"The combination of twelve s = ½ aza-closo-dodecaboran-1-yl radical units (NB11H11.) produces a supericosahedron [(NB11H6.)12](S), being S the total spin of the system. This work describes the study of the low-lying energy spin-projected states of this supericosahedron with two different geometrical arrangements: each Nitrogen atom pointing (i) inwards or (ii) outwards with respect to the radial axis of the supericosahedron. These spin-projected states are fitted into a Heisenberg spin Hamiltonian what allows one the calculation of coupling constants between magnetic sites. The eigenvalues of this model Hamiltonian then predict the ground spin state and the prefereed combinations of spin orientations of the magnetic centers. We show that the energy minimum in the [(Nin\/outB11H6.)12](S) systems corresponds to a high-spin S = 6 state.","palabras_clave":"Boron-Nitrogen compounts|Spin populations|Heisenberg spin Hamiltonians|Heisenberg cupling constants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4599,"idioma_id":4,"titulo":"Erratum to: Toward (car) borane-based molecular magnets","resumen":"In the original publication of the article, misleading fragment labels of (car)borane-based molecular compounds appear in the abstract as well as in the text. In order to build the polyradical carborane systems, we always start with the well-known neutral radical CB11H(.)12. These units are linked through -CH2- bridge units, after removing hydrogen atoms from the carborane cages. The correct description for fragments and formulae of the carborane polyradicals is given in Table 1.","palabras_clave":"-"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4600,"idioma_id":4,"titulo":"Performance of Shannon-Entropy Compacted N-Electron Wave Functions for Configuration Interaction Methods","resumen":"The coefficients of full configuration interaction wave functions (FCI) for N-electron systems expanded in N-electron Slater determinants depend on the orthonormal one-particle basis chosen although the total energy remains invariant . Some bases result in more compact wave functions, i.e. result in fewer determinants with significant expansion coefficients. In this work, the Shannon entropy, as a measure of information content, is evaluated for such wave functions to examine whether there is a relationship between the FCI Shannon entropy of a given basis and the performance of that basis in truncated CI approaches. The results obtained for a set of randomly picked bases are compared to those obtained using the traditional canonical molecular orbitals, natural orbitals, seniority minimising orbitals and a basis that derives from direct minimisation of the Shannon entropy. FCI calculations for selected atomic and molecular systems clearly reflect the influence of the chosen basis. However, it is found that there is no direct relationship between the entropy computed for each basis and truncated CI energies.","palabras_clave":"ENTROPY|CONFIGURATION INTERACTION|SENIORITY|DOCI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4601,"idioma_id":4,"titulo":"SYNTHESIS AND MECHANICAL PROPERTIES OF LAMINATES  BASED ON PHENOLIC RESINS MODIFIED WITH SODIUM LIGNOSULFONATE","resumen":"The reduction of phenol and\/or formaldehyde consumption during phenolic resin synthesis is of great technological and scientific interest because of its economic and environmental implications.In this work, the addition of sodium lignosulfonate as par-tial replacement of phenol in the phenolic resol base resins used for decorative laminates production is experimentally studied. The work involves: i) the characterization and reactivation of lignosulfonate, ii) the industrial synthesis of traditional and modified resoles by replacement of 10%w\/w of phenol, iii) the  industrial impregnation of Kraft-type papers with the produced resins, iv) the pro-duction of laminates at laboratory and industrial scale, and, v) the measurement of their final properties. Tensile, bending and impact strength were evaluated. Modified laminates exhibited mechanical properties statistically comparable with those of traditional laminates.Industrial tests were carried out at Centro S.A, San Francisco, Córdoba.","palabras_clave":"Laminate|Resol|Lignosulfonate|Mechanical Properties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4602,"idioma_id":4,"titulo":"Atom and bond Fukui functions and matrices: A Hirshfeld-I atoms-in-molecule approach","resumen":"The Fukui function is often used in its atom condensed form by isolating it from the molecular Fukui function using a chosen weight function for the atom in the molecule. Recently, Fukui functions and matrices for both atoms and bonds separately were introduced for semiempirical and ab initio levels of theory using Hückel and Mulliken atoms-in-molecule models. In this work, a double partitioning method of the Fukui matrix is proposed within the Hirshfeld-I atoms-inmolecule framework. Diagonalizing the resulting atomic and bond matrices gives eigenvalues and eigenvectors (Fukui orbitals) describing the reactivity of atoms and bonds. The Fukui function is the diagonal element of the Fukui matrix and may be resolved in atom and bond contributions. The extra information contained in the atom and bond resolution of the Fukui matrices and functions is highlighted. The effect of the choice of weight function arising from the Hirshfeld-I approach to obtain atom and bond condensed Fukui functions is studied. A comparison of the results with those generated using the Mulliken atoms-in-molecule approach shows low correlation between the two partitioning schemes.","palabras_clave":"FUKUI FUNCTION|FRONTIER MOLECULAR ORBITAL|ATOMS IN MOLECULES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4603,"idioma_id":4,"titulo":"Promising Efficacy of Benznidazole Nanoparticles in acute Trypanosoma cruzi Murine Model: In-Vitro and In-Vivo Studies","resumen":"The aim of this study was to evaluate the effectiveness of benznidazole nanoparticles (BNZ-nps) ontrypomastigote forms and on intracellular infection in mammalian cells and primary cardiac myocyte cells. Its effectivenesswas also evaluated on acute Trypanosoma cruzi Nicaragua mice infection. Trypomastigotes from culture weretreated with different concentrations of BNZ-nps to determine the drug concentration that lyses 50% of trypomastigotes(LC50). Infected mammalian cells were incubated with different concentrations of BNZ-nps to determine the percentageof amastigote inhibition. C3H\/HeN mice with lethal acute infection were treated with 10, 25, and 50 mg\/kg\/day of BNZnpsfor 30 and 15 days to control the survival rate of animals. BNZ-nps having a mean particle size of 63.3 nm, a sizedistribution of 3.35, and a zeta potential of &#8722;18.30 were successfully prepared using poloxamer 188 as a stabilizer.BNZ-nps 25 and 50 &#956;g\/mL showed no significant differences in the percentage of inhibition of infected mammaliancells. Infected mice treated with BNZ-nps (50, 25, and 10 mg\/kg\/day) for 30 days and with BNZ-nps (50 and 25 mg\/kg\/day)for 15 days presented a 100% survival, whereas the animals treated with 10 mg\/kg\/day for 15 days of BNZ-nps showeda 70% survival rate. The results obtained demonstrate, for the first time, that benznidazole nanoparticles are a usefuland attractive approach to treat Chagas disease in infected mice.","palabras_clave":"MURINE MODEL|TRYPANOSOMA CRUZI|BENZNIDAZOLE|NANOPARTICLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4604,"idioma_id":4,"titulo":"Photodynamic inactivation of Candida albicans by a tetracationic tentacle porphyrin and its analogue without intrinsic charges in presence of fluconazole","resumen":"The photodynamic inactivation mediated by 5,10,15,20-tetrakis[4-(3-N,N-dimethylaminopropoxy)phenyl]porphyrin (TAPP) and 5,10,15,20-tetrakis[4-(3-N,N,N-trimethylaminepropoxy)phenyl]porphyrin (TAPP4+) were compared in Candida albicans cells. Astrong binding affinity was found between these porphyrins and the yeast cells. Photosensitizedinactivation of C. albicans increased with both photosensitizer concentration and irradiation time.After 30 min irradiation, a high photoinactivation (&#8764;5 log) was found for C. albicans treated with 5 Mporphyrin. Also, the photoinactivation of yeast cells was still elevated after two washing steps. However,the photocytotoxicity decreases with an increase in the cell density from 106to 108cells\/mL. The highphotodynamic activity of these porphyrins was also established by growth delay experiments. This C.albicans strain was susceptible to fluconazole with a MIC of 1.0 g\/mL. The effect of photosensitizationand the action of fluconazole were combined to eradicate C. albicans. After a PDI treatment with 1 Mporphyrin and 30 min irradiation, the value of MIC decreased to 0.25 g\/mL. In addition, a completearrest in cell growth was found by combining both effects. TAPP was similarly effective to photoinactivateC. albicans than TAPP4+. This porphyrin without intrinsic positive charges contains basic amino groups,which can be protonated at physiological pH. Moreover, an enhancement in the antifungal action wasfound using both therapies because lower doses of the agents were required to achieve cell death.","palabras_clave":"PORPHYRIN|PHOTOINACTIVATION|ANTIFUNGAL|CANDIDA ALBICANS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4605,"idioma_id":5,"titulo":"Determinación de la humedad de suelo mediante regresión lineal múltiple con datos TerraSAR-X","resumen":"Los primeros 5 centímetros del suelo forman una interfaz donde se producen los principales intercambios de flujos de calor entre la superficie terrestre y la atmósfera. La teledetección ha demostrado ser una excelente fuente de datos espacial y temporalmente distribuidos de las características sobresalientes de la superficie terrestre. Los sensores de microondas activos (SAR) ofrecen la posibilidad de monitorizar la  humedad de suelo (HS) a escala global, regional y local, mediante la modelación de los procesos involucrados. Comúnmente, estos modelos se basan en la física de la retrodispersión de las ondas SAR, muy afectadas por las propiedades del suelo. Hasta el presente se han desarrollado muchas metodologías que estiman  HS a partir de técnicas estadísticas tales como redes neuronales, métodos de inversión y modelos de regresión. Estudios recientes han demostrado que las técnicas simples de regresión múltiple arrojan resultados aceptables. Las variables implicadas en estas metodologías son descriptivas de las estructura del suelo, características de las microondas y cobertura del suelo. Por esto, en este trabajo se propone desarrollar un modelo de regresión lineal múltiple para estimar HS en zonas de llanura combinando datos de la misión satelital TerraSAR-X y datos de una estación meteorológica. La modelación propuesta involucra las variables hidrológicas que caracterizan las zonas de llanura, donde los movimientos verticales de agua en el suelo predominan sobre el escurrimiento horizontal.  Los resultados obtenidos muestran que la selección a priori de las variables a involucrar es clave para determinar la variabilidad de HS en llanuras.","palabras_clave":"humedad del suelo|regresión múltiple|TERRASAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4606,"idioma_id":4,"titulo":"Reprogramming human A375 amelanotic melanoma cells by catalase overexpression: Upregulation of antioxidant genes correlates with regression of melanoma malignancy and with malignant progression when downregulated","resumen":"*These authors contributed equally to this work.Reactive oxygen species (ROS) are implicated in tumor transformation. Theantioxidant system (AOS) protects cells from ROS damage. However, it is alsohijacked by cancers cells to proliferate within the tumor. Thus, identifying proteins altered by redox imbalance in cancer cells is an attractive prognostic and therapeutic tool. Gene expression microarrays in A375 melanoma cells with different ROS levels after overexpressing catalase were performed. Dissimilar phenotypes by differential compensation to hydrogen peroxide scavenging were generated. The melanotic A375-A7 (A7) upregulated TYRP1, CNTN1 and UCHL1 promoting melanogenesis.The metastatic A375-G10 (G10) downregulated MTSS1 and TIAM1, proteins absentin metastasis. Moreover, differential coexpression of AOS genes (EPHX2, GSTM3,MGST1, MSRA, TXNRD3, MGST3 and GSR) was found in A7 and G10. Their increasein A7 improved its AOS ability and therefore, oxidative stress response, resembling less aggressive tumor cells. Meanwhile, their decrease in G10 revealed a disruption in AOS and therefore, enhanced its metastatic capacity.These gene signatures, not only bring new insights into the physiopathology ofmelanoma, but also could be relevant in clinical prognostic to classify between non aggressive and metastatic melanomas.","palabras_clave":"MELANOMA|AOS NETWORK|MELANOGENESIS|METASTASIS|MICROARRAYS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4607,"idioma_id":4,"titulo":"Antibacterial activity of essential oils of Aloysia polystachya and Lippia turbinata   (Verbenaceae)","resumen":"Aloysia polystachya and Lippia turbinata are medicinal and aromatic plants. Nevertheless, there are few reports in literature concerning the biological properties of species that grow in northeastern Argentina. The antibacterial activity and the chemical composition of both essential oils were evaluated in this work. The extraction was performed by steam distillation and their volatile compounds were determined by gas chromatography\/mass spectrometry. The antibacterial activity was evaluated by disc diffusion and broth microdilution assay. The main compounds were carvone (78.9%) and limonene (14.2%) in A. polystachya and carvone (80.77%), limonene (8.73%), &#946;-caryophyllene (2.13%) and 1,8-cineole (1.70%) in L. turbinata. Both essential oils were bactericide against Escherichia coli ATCC 35218 and clinical isolates of Enterobacter cloacae and Klebsiella pneumoniae. Essential oil of A. polystachya was also bactericide against Staphylococcus aureus ATCC 29212, S. aureus ATCC 25923 and clinical strain of S. aureus methicillin susceptible.","palabras_clave":"AROMATIC PLANTS|TERPENE|CARVONE|LIMONENE|BETA-CARYOPHYLLENE|1-8-CINEOLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4608,"idioma_id":5,"titulo":"Respuesta de una población de Solanum berthaultii Hawkes frente a Phytophthora infestans y su relación con la densidad y tipo de tricomas","resumen":"Ciento siete genotipos de Solanum berthaultii (ber)  provenientes del cruzamiento de los genotipos OKA 5880.22 y OKA 5632.11 del Banco de Germoplasma de la EEA Balcarce y los progenitores se evaluaron con el objetivo de determinar la variabilidad de la población frente a P. infestans, el tipo y la densidad de tricomas y la relación entre las variables. Se calculó el área bajo la curva de progreso de la enfermedad (AUDPC) para cada genotipo. Se realizaron recuentos de tricomas en dos estadíos fenológicos, cuando la planta tenía entre cuatro y cinco hojas desarrolladas y en madurez fisiológica, a los 90 días después de plantación. Se observaron diferencias significativas (p<0.05) en el valor de AUDPC entre los genotipos evaluados. Se concluyó que hay variabilidad para la densidad y tipo de tricomas y para el comportamiento frente a tizón tardío, lo cual indica que existe variabilidad fenotípica para estos caracteres en esta población de S.berthaultii. No hay correlación entre densidad y tipo de tricomas y la incidencia al tizón tardío en S. berthaultii. Por ello existe un buen potencial para el uso de esta especie para mejoramiento por estas características.","palabras_clave":"PAPA|TIZON|TRICOMAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4609,"idioma_id":4,"titulo":"Freshwater fishes of Patagonia: conservation and fisheries","resumen":"The absence of much literature on the Patagonian fish fauna in comparison with that of the neotropics, has previously been blamed on its poor species diversity. Knowledge of the fishes of Patagonia, however,rose sharply at the beginning of the present century, allowing for an understanding of the complex biogeographical history that has led to the present diversity and distribution patterns. There are several new and potential threats to biodiversity and conservation of Patagonian fishes, such as the introduction of exotic species, damming, climate change and changes geared to safeguard economic interests, often acting synergistically. A great amount of new information is now available and the aim of the present review is to articulate this knowledge in a comprehensive way in order to aid in the development of tools to face the increasing challenges posed by environmental change and human activity. Knowledge about fishes of Patagonia has grown at the same time as human actions, and presence.","palabras_clave":"Fishes |Lakes|Reseroirs|Rivers|Salmonids|Southern South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4610,"idioma_id":5,"titulo":"La guía ISO 26.000 y su importancia en la protección de la biodiversidad en Argentina","resumen":"LATINDEX CATÁLOGO (cumple requisitos 20 y 21) -  El objetivo del presente trabajo es analizar el aporte especial que podría realizarse desde la aplicación de normas voluntarias, en este caso de acuerdo con la guía de Responsabilidad Social ISO 26.000, específicamente en la protección de la biodiversidad en Argentina. A la vez, nos proponemos detectar aspectos que podrían considerarse de gran relevancia, en tanto que esta norma voluntaria podría complementar algunas lagunas jurídicas. Desde el punto de vista metodológico, se utilizó el método de observación directa y el analítico, y los datos cualitativos recogidos fueron secundarios. El principal aporte de este trabajo puede traducirse en la voluntad de fomentar la continua formación de la ciudadanía, en general, sobre cuestiones vinculadas con la sustentabilidad y la protección del ambiente y de la biodiversidad. Se abordó un enfoque interdisciplinario, ya que se realizó un análisis de tres importantes herramientas: la guía de Responsabilidad Social ISO 26.000, los instrumentos internacionales y las normas jurídicas ambientales locales cuya finalidad es la protección de la biodiversidad y del ambiente.","palabras_clave":"NORMAS|VOLUNTARIAS|RESPONSABILIDAD|SOCIAL|BIODIVERSIDAD|DERECHO|AMBIENTAL|INTERNACIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4611,"idioma_id":4,"titulo":"Atherogenic Lipoproteins in Subclinical Hypothyroidism and Their Relationship with Hepatic Lipase Activity: Response to Replacement Treatment with Levothyroxine","resumen":"BACKGROUND:Qualitative lipoprotein changes, such as an increase in fasting remnants, are reported in subclinical hypothyroidism (SCH). It was hypothesized that such changes are due to reduced hepatic lipase (HL) activity in SCH: HL is an enzyme regulated by thyroid hormones, and is involved in the degradation of triglyceride (TG)-rich remnants. This study aimed to quantify remnant-like lipoproteins (RLP), small dense LDL (sdLDL), and HL activity in women with SCH, and to assess these parameters after levothyroxine replacement therapy.METHODS:This was an observational cross-sectional study with a subsequent longitudinal follow-up. Findings in women with thyrotropin levels >4.5&#8201;mIU\/L (SH group) were compared with age- and body mass index (BMI)-matched euthyroid women (control group). In addition, a subgroup analysis was undertaken in SCH women who chose to receive levothyroxine treatment (0.9&#8201;&#956;g\/kg\/day) for 6 months. RLP was quantified by measuring cholesterol (RLP-C) and triglycerides (RLP-TG) after immunoaffinity chromatography, and sdLDL by automated standardized methods; HL activity was measured in post-heparin plasma.RESULTS:The SCH group included 37 women; 29 women were included in the control group. In addition, 22 women with SCH were included in the subgroup analysis (levothyroxine treatment). Significantly higher RLP values were observed in the SCH group than in the control group: RLP-C (median [range], mg\/dL): 20.3 (5.8-66.8) versus 10.2 (2.7-36.3), p&#8201;=&#8201;0.005; RLP-TG (mg\/dL): 26.3 (3.2-123.3) versus 12.1 (2.5-61.6), p&#8201;=&#8201;0.033. HL activity (mean ± standard deviation [SD], &#956;mol free fatty acid\/mL post-heparin plasma.h)-9.83&#8201;±&#8201;4.25 versus 9.92&#8201;±&#8201;5.20, p&#8201;=&#8201;0.707-and sdLDL levels (mg\/dL)-23.1&#8201;±&#8201;10.7 versus 22.6&#8201;±&#8201;8.4, p&#8201;=&#8201;0.83-were similar. After levothyroxine, RLP-C decreased-21.5 (5.8-66.8) versus 17.2 (4.1-45.6), p&#8201;=&#8201;0.023-and HL increased-9.75&#8201;±&#8201;4.04 versus 11.86&#8201;±&#8201;4.58, p&#8201;=&#8201;0.012-in the subgroup of SCH women. No changes in sdLDL were observed.CONCLUSIONS:Women with SCH have higher RLP levels than matched controls do, but their RLP-C levels decrease significantly following levothyroxine therapy. Furthermore, HL activity also increases after levothyroxine therapy and can be interpreted as a possible explanation for the decrease in RLP-C.","palabras_clave":"SUBCLINICAL HYPOTHYROIDISM|HEPATIC LIPASE|LEVOTHYROXINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4612,"idioma_id":4,"titulo":"SPATIAL STRUCTURE OF SKIN FOLLICLES IN SURI AND HUACAYA ALPACAS","resumen":"The present study aimed at characterizing the type and arrangement of skin follicles of Suri and Huacaya alpaca. Samples (11 Suri and 10 Huacaya) were collected by punch skin biopsy from the midside of alpaca and processed for histological study. Each biopsy was examined using projection microscope. Follicular groups were identified and the position of each secondary and primary follicle was recorded. The ratio of secondary to primary follicles (S\/P ratio) was compared between breeds using the Wilcoxon test. The spatial structure of the follicles was analyzed with Ripley?s K function and the L function. To detect deviations from Complete Spatial Randomness at different spatial scales simulated confidence enveloipes were calculated. The S\/P ratio did not differ between Huacaya (7.1± 0.52) and Suri (7.21 ± 0.62). There is evidence of statistically significant spatial structure of the follicles in both breeds at small spatial scales. However, at a higher spatial scale, the proportion of samples with a clustered spatial structure of follicles was significantly higher in Huacaya. The study of skin follicles spatial pattern opens up new possibilities for improving knowledge of the potential role of skin follicle in alpaca fibre production.","palabras_clave":"FIBRE PRODUCTION|CAMELIDS|POINT PATTERN ANALYSIS|RIPLEY'S K FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4613,"idioma_id":4,"titulo":"Catalyst- and solvent-free hydrophosphination and multicomponent hydrothiophosphination of alkenes and alkynes","resumen":"The hydrophosphination of carbon?carbon multiple bonds has been generally performed under acid, base or metal catalysis in different solvents. Herein, alkyl and alkenyl tertiary phosphines are obtained by the addition of diphenylphosphine to alkenes and alkynes, respectively, in the absence of a solvent and a catalyst. In the presence of elemental sulfur, the corresponding alkyl and alkenyl tertiary phosphine sulfides are synthesized in a three-component process. These simple methods, which meet most of the principles of Green Chemistry, are highly regioselective towards the anti-Markovnikov products and diastereoselective towards the Z alkenyl phosphines. The mechanistic aspects of the reactions are also tackled and the efficiency of the latter is compared with that of the catalytic methods.","palabras_clave":"HYDROPHOSPHINATION|HYDROTHIOPHOSPHINATION|CATALYST AND SOLVENT FREE|ALKENES AND ALKYNES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4614,"idioma_id":5,"titulo":"PROGRAMA DE ESTUDIOS SOBRE FUNDAMENTOS TEÓRICO-METODOLÓGICOS  DEL TRABAJO SOCIAL (PEFTS)","resumen":"La creación del Programa de Estudios sobre Fundamentos Teórico-Metodológicos del Trabajo Social (PEFTS) surge a partir del desarrollo de actividades de investigación, docencia y extensión o transferencia que desde distintos proyectos de investigación radicados en el Departamento de Ciencias Sociales de la UNLu se desarrollaban desde el año 2002. Las líneas de investigación sobre las que se viene trabajando desde el inicio del Programa son: Desarrollo histórico del Trabajo Social (Historia del Trabajo Social); Debate contemporáneo del Trabajo Social; Fundamentos y características de la intervención profesional. Esta última línea de investigación a su vez se desdobla en diversas áreas temáticas como: Políticas Sociales; Salud; Niñez y Familia; Salud Mental y Comunitaria; etc. Estas líneas de trabajo responden a los objetivos que orientan las diversas actividades emprendidas desde el Programa:  - elaborar y desarrollar proyectos de investigación pertinentes a las líneas de investigación propuestas y las demandas sociales del país y la región; - contribuir a la formación de becarios, tesistas e investigadores; - promover la difusión de los resultados de las investigaciones, la producción de publicaciones y la transferencia de conocimientos a la sociedad; - brindar asesoramiento y asistencia técnica dentro del área de competencia; - contribuir con la Secretaría de Investigación del Departamento de Ciencias Sociales al desarrollo de actividades de perfeccionamiento y actualización de los investigadores; - promover la articulación y convenios con equipos de investigación, universidades, centros de estudios y redes nacionales e internacionales.","palabras_clave":"Investigación|Fundamentos|Trabajo Social"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4615,"idioma_id":4,"titulo":"Cholesterol induces surface localization of polyphenols in model membranes thus enhancing vesicle stability against lysozyme, but reduces protection of distant double bonds from reactive-oxygen species","resumen":"The main scope of the present study was to analyze the membrane interaction of members of different classes of polyphenols, i. e. resveratrol, naringenin, epigallocatechin gallate and enterodiol, in model systems of different compositions and phase states. In addition, the possible association between membrane affinity and membrane protection against both lipid oxidation and bilayer-disruptive compounds was studied.Gibbs monolayer experiments indicated that even though polyphenols showed poor surface activity, readily interacted with lipid films. Actually, a preferential interaction with expanded monolayers was observed, while condensed and cholesterol-containing monolayers decreased the affinity of these phenolic compounds. On the other hand, fluorescence anisotropy studies showed that polyphenols were able to modulate membrane order degree, but again this effect was dependent on the cholesterol concentration and membrane phase state. In fact, cholesterol induced a surface rather than deep into the hydrophobic core localization of phenolic compounds in the membranes.In general, the polyphenolic molecules tested had a better antioxidant activity when they were allowed to get inserted into the bilayers, i.e. in cholesterol-free membranes. On the other hand, a membrane protective effect against bilayer permeabilizing activity of lysozyme, particularly in the presence of cholesterol, could be assessed. It can be hypothesized that phenolic compounds may protect membrane integrity by loosely covering the surface of lipid vesicles, once cholesterol push them off from the membrane hydrophobic core. However, this cholesterol-driven distribution may lead to a reduced antioxidant activity of linoleic acid double bonds.","palabras_clave":"POLYPHENOLS|LANGMUIR MONOLAYERS|LIPOSOMES|MEMBRANE ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4616,"idioma_id":4,"titulo":"Acceptability and suitability of Tuta absoluta eggs from irradiated parents to parasitism by Trichogramma nerudai and Trichogramma pretiosum (Hymenoptera: Trichogrammatidae)","resumen":"Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most devastating pests of tomato crops. We studied the acceptability and suitability of eggs laid by irradiated T. absoluta pairs to parasitism by the parasitoids Trichogramma nerudai and Trichogramma pretiosum.Trichogramma absoluta pupae were irradiated with X-radiation (20834 R) and the emerged adults were separated into couples according to the crosses: &#9794;U&#8201;×&#8201;&#9792;U, &#9794;I&#8201;×&#8201;&#9792;U, &#9794;U&#8201;×&#8201;&#9792;I (where ?U? is untreated and ?I? is irradiated). In a no-choice experiment, 40 eggs from each cross were exposed to a female parasitoid for 24&#8201;h.All T. absoluta eggs were accepted for oviposition by T. nerudai and were suitable for its development. However, eggs from irradiated females were significantly less parasitized than those from untreated females. Trichogramma pretiosum showed low parasitism on eggs from all crosses. In a choice experiment, both T. nerudai and T. pretiosum showed no differences in the parasitism of eggs from irradiated (n&#8201;=&#8201;40) and non-irradiated female moths (n&#8201;=&#8201;40).These results indicate that eggs laid by irradiated parents were acceptable for oviposition and suitable for the development of these parasitoids, and also support the idea that the inherited sterility technique could be used in an integrated approach with egg parasitoids to control T. absoluta.","palabras_clave":"BIOLOGICAL CONTROL|EGG PARASITOID|INHERITED STERILITY|NATURAL ENEMIES|TOMATO LEAF MINER|X-RAY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4617,"idioma_id":4,"titulo":"Ultrastructural study of the female gametophyte and the epistase in Cabombaceae and Nymphaeaceae","resumen":"Ultrastructural studies on the female gametophyte arerestricted to species at relatively derived positions in the angiospermphylogenetic tree. Therefore, this topic remains mostly unknown for the early divergentlineages, in which a four-celled megagametophyte is common. Here,ultrastructure of the megagametophyte and micropylar nucellar epidermis wasinvestigated in Cabomba caroliniana A. Gray (Cabombaceae), Nymphaea gardnerianaPlanch. and Victoria cruziana Orb. (Nymphaeaceae). The micropylar nucellarepidermis of the studied species differentiates into an epistase. These cellshave metabolically active cytoplasm and thickened inner tangential walls.Epistase ultrastructure is compatible with a transfer cell specialization. Thistissue may play an adaptive role in the secretion of chemotropic substances todirect the pollen tube growth toward the female gametophyte. The cytologicalcharacteristics of the female germ unit in members of Cabombaceae andNymphaeaceae are generally similar to other angiosperms that develop a typicalseven-celled, eight-nucleate female gametophyte; however, they differ in somespecific points. In V. cruziana and N. gardneriana, the micropylar end of thesynergids develops a rudimentary filiform apparatus with slight inwardprojections. By contrast, the synergids lack a filiform apparatus in C.caroliniana. Unlike most studied angiosperms, the filiform apparatus in theclade Cabombaceae-Nymphaeaceae is underdeveloped or absent, therefore characterstate transformations have occurred within basal angiosperms. The potentialevolutionary shifts of this reproductive feature are highlighted.","palabras_clave":"CABOMBA CAROLINIANA|FILIFORM APPARATUS|NYMPHAEA GARDNERIANA|TRANSFER CELL|ULSTRASTRUCTURE|VICTORIA CRUZIANA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4618,"idioma_id":4,"titulo":"Are the Oxygen isotopic compositions of Fitzroya cupressoides and Nothofagus pumilio cellulose promising proxies for climate reconstructions in northern Patagonia?","resumen":"Tree ring &#948;18O chronologies from two native species (Fitzroya cupressoides and Nothofagus pumilio) in northern Patagonia were developed to assess their potential for paleoclimate reconstructions. The five annually resolved cellulose &#948;18O chronologies (two for F. cupressoides and three for N. pumilio) are located on the Andes along the steep west-to-east precipitation gradient. Over the common 60 years long interval, the five site-&#948;18Ocell chronologies exhibit a strong common signal as indicated by the significant mean intercorrelation (r = 0.61, p<0.05) and the high percentage (65%) of total variance explained by the first empirical orthogonal function. Although correlation analyses reveal that the two mean species-&#948;18Ocell chronologies are mainly modulated by December?May temperature, the N. pumilio chronology shows a greater sensitivity to record temperature variations (r = 0.57, p<0.05). The &#948;18Ocell of N. pumilio contains a regional temperature signal representative of a large area in southern South America under the influence of the Southern Annular Mode. This study indicates that &#948;18Ocell in N. pumilio is a promising proxy to reconstruct past variations in temperature in South America south of 38°S.","palabras_clave":"isotopic climate signal|northern Patagonia|dendrochronology|Fitzroya cupressoides|Nothofagus pumilio"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4619,"idioma_id":5,"titulo":"ESTRUCTURAS DE SEGREGACION VESICULADAS ALOJADAS EN COLADAS BASALTICAS PAHOEHOE DE PAYENIA, PROVINCIAS DE MENDOZA Y LA PAMPA, ARGENTINA: ASPECTOS MORFOGENETICOS Y PETROGRAFICOS","resumen":"Las estructuras de segregación vesiculadas comprenden cuerpos de variada geometría y dimensiones milimétricas a métricas con una vesiculación de entre el 20 y el 40 % alojados en los sectores internos de un flujo lávico. Estas resultan de la segregación y expulsión de un diferenciado residual rico en volátiles generado durante la cristalización de la colada. El residuo migra atravesando una red permeable de cristales gracias al mecanismo de filtrado por presión de gas. Según sus características microtexturales, las estructuras extraídas de coladas basálticas pahoehoe de Payenia fueron agrupadas en tres subtipos: afíricas de grano grueso, afíricas de grano fino y porfíricas a intergranulares. Los términos de grano grueso se asocian a la presencia de iddingsita de alta temperatura en el olivino del basalto hospedante y la textura diktitaxítica es un rasgo petrográfico común de todas las coladas muestreadas. Ambos aspectos constituyen la evidencia de una importante participación de fluidos, posiblementede origen magmático, durante la formación de estas estructuras. Se describieron cuatro tipos de estructuras: mantos de vesículas, láminas de vesículas, cilindros de vesículas y vesículas de segregación, las cuales fueron agrupadas según su morfogénesis, en categorías propuestas en trabajos previos basadas en el espesor del flujo lávico hospedante. El origen de las estructurasmantiformes estaría asociado a la dispersión de cilindros de vesículas debajo de un frente de solidificación. La tasa de enfriamiento de la colada está regulada por el espesor del flujo, lo que a su vez condiciona la permanencia o dispersión total de las estructuras cilíndricas.","palabras_clave":"Cilindros de vesículas|Mantos de vesículas|Vesículas de segregación|Basaltos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4620,"idioma_id":4,"titulo":"Establishment of cell-based transposon-mediated transgenesis in cattle","resumen":"Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes (fluorophore and neomycin resistance). Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transposition is a feasible approach for genetic engineering in the cattle genome.","palabras_clave":"Transposones|Transgénicos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4621,"idioma_id":4,"titulo":"Development of a Non-ionic Azobenzene Amphiphile for Remote Photocontrol of a Model Biomembrane","resumen":"We report the synthesis and characterization of a simple non-ionic Azoamphiphile, C12OazoE3OH which behaves as an optically-controlled molecule alone and in a biomembrane-environment. First, Langmuir monolayer and Brewster angle microscopy (BAM) experiments showed that pure C12OazoE3OH enriched in the (E) isomer was able to form solid-like mesophase even at low surface pressure associated with supramolecular organization of the azobenzene derivative at the interface. On the other hand, pure C12OazoE3OH enriched in the (Z) isomer formed a less solid-like monolayer due to the bent geometry around the azobenzene moiety. Second, C12OazoE3OH is wellmixed in a biological membrane model, Lipoids75TM (up to 20 %mol), and photoisomerization among the lipids proceeded smoothly depending on light conditions. It is proposed that the crosssectionalarea of the hydroxyl triethylenglycol head of C12OazoE3OH inhibits azobenzenes Haggregation in the model membrane, thus the tails conformation change due to photoisomerization is transferred efficiently to the lipid membrane. We showed that the lipid membrane effectively senses the azobenzene geometrical change photo-modulating some properties, like compressibility modulus, transition temperature and morphology. In addition, photomodulation proceeds with acolor change from yellow to orange, providing the possibility to externally monitoring the system. Finally, Gibbs monolayers showed that C12OazoE3OH is able to penetrate the highly packing biomembrane model, thus C12OazoE3OH might be used as photoswitchable molecular probe in real systems.","palabras_clave":"LANGMUIR FILMS|BREWSTER ANGLE MICROSCOPY|DIFFERENTIAL CALORIMETRY|LIPID MEMBRANES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4622,"idioma_id":5,"titulo":"Comunidades autónomas de producción de conocimiento: jerarquías, conflictos y redefiniciones","resumen":"Trabajo que presenta el caso de una comunidad de edición amateur de libros electrónicos, ilustrando la secuencia de creación, crecimiento, crisis y reformulación del proyecto original.","palabras_clave":"comunidades virtuales|produccion de conocimiento|conflicto"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4623,"idioma_id":4,"titulo":"'Gregaria' to 'subrugosa', that is the question: shape changes under laboratory conditions in the pelagic morph of the squat lobster Munida gregaria (Anomura, Munididae)","resumen":"Munida gregaria (Fabricius 1793) is one of the few species of the family Munididae withtwo morphs, one with a post-metamorphic pelagic phase that eventually settles to thesea-bottom and the other being exclusively benthic, known as 'gregaria' and'subrugosa'. This study provides the first information on growth of the pelagic morph ofMunida gregaria and its relation with changes in morphology in southern SouthAmerica. Specifically, we studied growth at molt and duration of the intermolt period inthe laboratory, and analyzed changes in shape through growth by means of geometricmorphometry techniques. Fifty squat lobsters were sampled from shoals in the BeagleChannel and kept in individual flasks during 29 months. The carapace of each exuviaewas photographed and analyzed using 2D geometric morphometry analysis. Changesin shape associated with growth in gregaria were gradual, adjusted to a linear function,and occurred mainly in the anterior zone of the carapace. However, after two yearsand at least 8 molts, individuals of the gregaria morph were still clearly distinguishablefrom a typical individual of the subrugosa morph. These results suggests that once thesexual maturity is attained, pelagic individuals of Munida gregaria do not transformfrom gregaria to subrugosa, and retain the gregaria morphology.","palabras_clave":"Beagle Channel|Decapoda|Geometric morphometry|Swarms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4624,"idioma_id":5,"titulo":"Obtención de recubrimientos porosos de TiO2 por oxidación anódica para aplicaciones fotocatalíticas","resumen":"El TiO2 es uno de los materiales más utilizados en el proceso de fotocatálisis heterogénea debido a su bajo costo, baja toxicidad y elevada actividad fotocatalítica.En el presente trabajo se sintetizaron recubrimientos de TiO2 por oxidación anódica, iniciando a densidad de corriente constante hasta un voltaje de 120 V, y continuando apotencial constante en condiciones de spark discharge. Luego de la oxidación, los recubrimientos se trataron térmicamente. La variable del proceso fue la densidad de corriente inicial. Las curvas de densidad de corriente en función del tiempo mostraron fluctuaciones propias del fenómeno de spark discharge. Las micrografías mostraron un diámetro de poro de aproximadamente 100 nm. Los difractogramas indicaron la presencia de las fases anatasa y rutilo del TiO2, con un incremento en la fracción rutilo al aumentar la densidad de corriente. Los óxidos sintetizados presentaron características favorables como material de potencial aplicación en procesos de fotocatálisis heterogénea.","palabras_clave":"dióxido de titanio|tratamiento de aguas|ácido sulfúrico|fotocatálisis heterogénea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4625,"idioma_id":5,"titulo":"Hongos micorrícico-arbusculares asociados a Chenopodiaceae en dos ambientes salinos de Córdoba","resumen":"Los hongos micorrícico-arbusculares (HMA) colonizan aproximadamente el 82% de las plantas terrestres en cualquier ecosistema, aún en condiciones extremas tales como los suelos salinos. En el presente trabajo se reporta la colonización micorrícica arbuscular (CMA) y su concentración radical en cuatro especies de Chenopodiaceae (Allenrolfea patagonica, Atriplex argentina, Heterostachys ritteriana y Suaeda divaricata) en cinco profundidades del suelo en dos salinas del Centro de Argentina. Todas las plantas mostraron colonización micorrícica que varió significativamente entre especies y sitios para todas las profundidades. Atriplex argentina presentó el mayor porcentaje de CMA en casi todas las profundidades (0-50 cm). En todas las especies, la concentración radical disminuyó a medida que se incrementó la profundidad en ambos sitios. La presencia de raíces micorrizadas en las capas más profundas del suelopara las cuatro especies de Chenopodiaceae, muestran que estos hongos son tolerantes a vivir en los suelos salinos del centro de Argentina.","palabras_clave":"HONGOS MICORRÍCICO ARBUSCULARES|AMBIENTES SALINOS|PERFIL DEL SUELO|DISTRIBUCIÓN VERTICAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4626,"idioma_id":4,"titulo":"Environmental preferences of brachiopods and bivalves across major climatic changes during the late Paleozoic ice age (Pennsylvanian, western Argentina)","resumen":"During the late Palaeozoic ice age (LPIA), ice-proximal marine regional communities record contrasting responses to climate change compared to ice-distal communities. However, there is still much to be understood in distal regions in order to have a comprehensive idea of the palaeobiological consequences of the LPIA. Here, were analyse brachiopod and bivalve environmental preferences along the bathymetric gradient during a major glacial event and the subsequent non glacial interval in western Argentina. Median environmental breadths did not change with the reassembly of communities during the non-glacial interval. Moreover, bivalves and brachiopods immigrants show similar environmental breadths although they tend to have immigrated from different palaeogeographical regions. These patterns reinforce the idea that the worldwide marine fauna was probably culled of stenotopic taxa during the LPIA. On the other hand, the analyses of survivors and immigrants preferred depths sheds light on the substantial modification of the bathymetric diversity gradient. Among different possible explanations, the immigration of taxa with affinities for deep environments is the only one supported. In addition, results underscore that the higher turnover in the offshore was probably driven by immigration rather than extinction. Finally, stability in environmental preferences at regional scale is not mirrored by stability in survivor?s individual preferences, because survivors preferred depth is not correlated during the glacial and non-glacial intervals. Moreover, the amount of change in survivors preferred depth is not related to their environmental breadth, nor to their occupancy. These patterns suggest (1) instability in realized niches and (2) individual responses of survivor genera.","palabras_clave":"ENVIRONMENTAL PREFERENCES|LPIA|CLIMATE|BRACHIOPODS|BIVALVES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4627,"idioma_id":4,"titulo":"Use of a critical phosphorus dilution curve to improve potato crop nutritional management","resumen":"Abstract Plant diagnostic methods to determine P deficienciesneed the definition of a critical concentration value whichrepresents the minimum P concentration required to achievemaximum growth. The critical P concentration (Pc) has notbeen determined for potato. We designed a five years experimentwith the objective of using the relationship between Pconcentrations and total dry matter (TB) yield to determineand validated the Pc, expressed as a function of TB. The Pcwas described by the model: 3.919*TB -0.304 where total biomass(TB) ranged from 1 to 16 Mg ha&#8722;1. The P nutritionalindex (PNI), which is the ratio between the measured wholeplant P concentration and Pc, was associated with the relativetuber yield measured at harvest. The results from this studysupport the use of PNI from 60 to 80 days after planting todetermine potato crop P nutritional status.","palabras_clave":"phosphorus nutrition index|Solanum tuberosum L.|fertilization |total biomass"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4628,"idioma_id":4,"titulo":"Endophytic fungi from selected varieties of soybean (Glycine max L. Merr.) and corn (Zea mays L.) grown in an agricultural area of Argentina","resumen":"Endophytic fungi areubiquitous and live within host plants without causing any noticeable symptomsof disease. Little is known about the diversity and function of fungalendophytes in plants, particularly in economically important species. The aimof this study was to determine the identity and diversity of endophytic fungiin leaves, stems and roots of soy-bean and corn plants and to determine theirinfection frequencies. Plants were collected in six areas of the provinces ofBuenos Aires and Entre Ríos (Argentina) two areas were selected for samplingcorn and four for soybean. Leaf, stem and root samples were surface-sterilized,cut into cm2 pieces using sterile scalpel and aseptically transferred to platescontaining potato dextrose agar plus antibiotics. The species were identifiedusing both morphological and molec-ular data. Fungal endophyte colonization insoybean and corn plants was influenced by tissue type and varieties whereas incorn plants only by tissue type. greater number of endophytes were isolatedfrom stem tissues than from leaves and root tissues in both species of plants.The most frequently isolated species in all soybean cultivars was Fusariumgraminearum and the least isolated one was Scopulariopsis brevicaulis.Furthermore, the most frequently isolated species in corn plants wasAspergillus terreus whereas the least isolated one was Aspergillus flavus.These results could be relevant in the search for endophytic fungi isolatesthat could be of interest in the control of agricultural pests","palabras_clave":"ENDOPHYTIC |FUNGI|SOYBEAN|CORN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4629,"idioma_id":4,"titulo":"First  record  of  Talaromyces  udagawae  in  soil  related to  decomposing  human  remains  in  Argentina","resumen":"The&nbsp; morphologic&nbsp; features&nbsp; of&nbsp; Talaromyces&nbsp; udagawae&nbsp; Stolk&nbsp; and&nbsp; Samson&nbsp; are&nbsp; heredescribed&nbsp; and&nbsp; illustrated.&nbsp; This&nbsp; teleomorphic&nbsp; Ascomycota&nbsp; fungus&nbsp; was&nbsp; isolated&nbsp; from&nbsp; soil&nbsp; obtainedin&nbsp; Buenos&nbsp; Aires&nbsp; province&nbsp; (Argentina)&nbsp; from&nbsp; beneath&nbsp; a&nbsp; human&nbsp; cadaver&nbsp; in&nbsp; an&nbsp; advanced&nbsp; state&nbsp; ofdecomposition.&nbsp; After&nbsp; washing&nbsp; and&nbsp; serial&nbsp; dilution&nbsp; of&nbsp; the&nbsp; soil&nbsp; along&nbsp; with&nbsp; moist-chamber&nbsp; techniques&nbsp; for&nbsp; fungal&nbsp; cultivation,&nbsp; T.&nbsp; udagawae&nbsp; formed&nbsp; very&nbsp; restricted&nbsp; colonies&nbsp; of&nbsp; bright&nbsp; yellowcolor&nbsp; on&nbsp; different&nbsp; growth&nbsp; media&nbsp; with&nbsp; 8-ascospored&nbsp; asci.&nbsp; The&nbsp; ascospores&nbsp; were&nbsp; ellipsoidal&nbsp; andornamented.&nbsp; The&nbsp; anamorphic&nbsp; state&nbsp; was&nbsp; not&nbsp; observed.&nbsp; Molecular-genetic&nbsp; techniques&nbsp; identifiedthe&nbsp; species.&nbsp; The&nbsp; present&nbsp; record&nbsp; is&nbsp; the&nbsp; first&nbsp; of&nbsp; the&nbsp; species&nbsp; in&nbsp; Argentina,&nbsp; pointing&nbsp; it&nbsp; as&nbsp; a&nbsp; tool&nbsp; toidentify&nbsp; soils&nbsp; where&nbsp; cadaver&nbsp; decomposition&nbsp; occurs.","palabras_clave":"Talaromyces udagawae|Hongos  de  suelo|Descomposición cadavérica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4630,"idioma_id":4,"titulo":"FullSSR: Microsatellite Finder and Primer Designer","resumen":"Microsatellites are genomic sequences comprised of tandem repeats of short nucleotide motifs widely used as molecular markersin population genetics. FullSSR is a new bioinformatic tool for microsatellite (SSR) loci detection and primer design usinggenomic data from NGS assay.The software was tested with 2000 sequences of Oryza sativa shotgun sequencing project from theNational Center of Biotechnology Information Trace Archive and with partial genome sequencing with ROCHE 454 fromCaimanlatirostris, Salvatormerianae, Aegla platensis, andZilchiopsis collastinensis. FullSSR performance was compared against other similarSSR search programs. The results of the use of this kind of approach depend on the parameters set by the user. In addition, resultscan be affected by the analyzed sequences because of differences among the genomes. FullSSR simplifies the detection of SSRs andprimer design on a big data set. The command line interface of FullSSR was intended to be used as part of genomic analysis toolspipeline; however, it can be used as a stand-alone program because the results are easily interpreted for a nonexpert user.","palabras_clave":"Microsatelites|SSR|Primer|Secuenciación de alto rendimiento"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4631,"idioma_id":5,"titulo":"Dosis óptima económica de fósforo en el cultivo de papa en el sudeste bonaerense","resumen":"Asegurar una buena disponibilidad de fósforo (P) para el cultivo de papa, es tan importante como ajustar la estrategia de fertilización para producir el máximo rendimiento con la mayor eficiencia económica. El Avail®, aditivo que puede aplicarse como recubrimiento de fertilizantes fosforados, ha demostrado aumentar la eficiencia de uso en diversos cultivos, sin resultados consistentes en papa. Para el cultivo de papa en el sudeste bonaerense, se plantearon como objetivos: 1- Comparar el rendimiento a dosis diferentes de superfosfato triple de calcio sin (SFT) y con agregado del Avail® (SFT+ Avail®) y 2- establecer la dosis de fertilización fosforada que produce el máximo beneficio económico (DOE). Se realizaron nueve ensayos de fertilización fosforada (2005-14). Se determinó contenido de P en el suelo con los extractantes Bray1 y Mehlich3 y el rendimiento de papa. Se calculó la DOE para diferentes relaciones de precios fertilizante\/tubérculo. La fertilización incrementó el rendimiento en todos los ensayos (44 y 52-69 Mg ha-1 para el testigo y los tratamientos fertilizados, respectivamente. No se detectaron diferencias significativas en rendimiento debido al uso de diferentes fuentes de P (SFT o SFT+Avail®), para ninguna de las dosis evaluadas. Esta respuesta fue consistente en todos los ensayos a pesar que tenían contenidos diferentes de P extractable. La DOE determinada a partir de Bray1 o Mehlich3 fueron similares (R2=0,8). Este resultado fue consistenteen todos los sitios con diferente P extraíble antes de la siembra. Para ambos extractantes, la DOE disminuyó con el aumento de la disponibilidad inicial de P según: DOE P (kg ha-1) = 176 -5 *Bray1; 216 -6 *Mehlich3. Si bien la disponibilidad de P en el suelo y la relación de precios afectaron la DOE, la primera fue el factor","palabras_clave":"Avail|Bray-Mehlich"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4632,"idioma_id":4,"titulo":"Cell wall-related genes studies on peach cultivars with differential susceptibility to woolliness: looking for candidates as indicators of chilling tolerance","resumen":"The results obtained indicate that a &#946;-xylosidase gene may act as good indicator of chilling tolerance and provide new insights into the complex issue of peach fruit woolliness. The storage of peaches at low temperatures for prolonged periods can induce a form of chilling injury (CI) called woolliness, characterized by a lack of juiciness and a mealy texture. As this disorder has been associated with abnormal cell wall dismantling, the levels of 12 transcripts encoding proteins involved in cell wall metabolism were analysed in cultivars with contrasting susceptibility to this disorder selected from five melting flesh peach cultivars. The resistant (´Springlady´) and susceptible (´Flordaking´) cultivars displayed differences in the level of expression of some of the selected genes during fruit softening and in woolly versus non-woolly fruits. From these genes, the level of expression of PpXyl, which encodes for a putative &#946;-xylosidase, was the one that presented the highest correlation (negative) with the susceptibility to woolliness. PpXyl expression was also analysed in a cultivar (´Rojo 2´) with intermediate susceptibility to woolliness, reinforcing the conclusion about the correlation of PpXyl expression to the presence of woolliness symptom. Moreover, the level of expression of PpXyl correlated to protein level detected by Western blot. Analyses of the promoter region of the PpXyl gene (1637 bp) isolated from the three cultivars showed no differences suggesting that cis-elements from other regions of the genome and\/or trans elements could be responsible of the differential PpXyl expression patterns. Overall, the results obtained indicate that PpXyl may act as a good indicator of woolliness tolerance and that the regulation of expression of this gene in different cultivars does not depend on sequences upstream the coding sequence.","palabras_clave":"Cell wall metabolism|Chilling injury|Peach|Prunus persica|Woolliness |Xylosidase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4633,"idioma_id":4,"titulo":"Tuning the electronic properties at the surface of BaBiO 3 thin films","resumen":"The presence of 2D electron gases at surfaces or interfaces in oxide thin films remains a hot topic in condensed matter physics. In particular, BaBiO3 appears as a very interesting system as it was theoretically proposed that its (001) surface should become metallic if a Bi-termination is achieved (Vildosola et al., PRL 110, 206805 (2013)). Here we report on the preparation by pulsed laser deposition and characterization of BaBiO3 thin films on silicon. We show that the texture of the films can be tuned by controlling the growth conditions, being possible to stabilize strongly (100)-textured films. We find significant differences on the spectroscopic and transport properties between (100)-textured and non-textured films. We rationalize these experimental results by performing first principles calculations, which indicate the existence of electron doping at the (100) surface. This stabilizes Bi ions in a 3+ state, shortens Bi-O bonds and reduces the electronic band gap, increasing the surface conductivity. Our results emphasize the importance of surface effects on the electronic properties of perovskites, and provide strategies to design novel oxide heterostructures with potential interface-related 2D electron gases.","palabras_clave":"thin films|BaBiO3|electronic effecta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4634,"idioma_id":4,"titulo":"Effects of high density on spacing behaviour and reproduction in Akodon azarae: A fencing experiment","resumen":"We studied the short term spacing behavioural responses of Pampean grassland mouse (males exhibit a fusion spatial response.Akodon azarae) with regard to population density in four 0.25 ha enclosures (two control and two experimental) in the 2011 breeding season. Based on the hypothesis that A. azarae breeding females exhibit spacing behaviour, and breeding males show a fusion spatial response, we tested the following predictions: (1) home range size and intrasexual overlap degree of females are independent of population density values; (2) at high population density, home range size of males decreases and the intrasexual home range overlap degree increases. To determine if female reproductive success decreases at high population density, we analyzed pregnancy rate, size and weight of litters, and period until fecundation in both low and high enclosure population density. We found that both males and females varied their home range size in relation to population density. Although male home ranges were always bigger than those of females in populations with high density, home range sizes of both sexes decreased. Females kept exclusive home ranges independent of density values meanwhile males decreased home range overlap in high breeding density populations. Although females produced litters of similar size in both treatments, weight of litter, pregnant rate and period until fecundation varied in relation to population density. Our results did not support the hypothesis that at high density females of A. azarae exhibit spacing behaviour neither that males exhibit a fusion spatial response.","palabras_clave":"SPACING BEHAVIOUR|DENSITY-EFFECT |NEOTROPICAL RODENTS|REPRODUCTIVE DELAY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4635,"idioma_id":4,"titulo":"Time-Lapse Evaluation of Interactions Between Biodegradable Mg Particles and Cells","resumen":"Mg-based implants have promising applications as biodegradable materials in medicine for orthopedic, dental, and cardiovascular therapies. During wear and degradation microdebris are released. Time-lapse multidimensional microscopy (MM) is proposed here as a suitable tool to follow, in fixed intervalsover 24-h periods, the interaction between cells and particles. Results of MM show interactions of macrophages (J774) with the magnesium particles (MgPa) that led to modifications of cell size and morphology, a decrease induplication rate, and cell damage. Corrosion products were progressively formed on the surface of the particles and turbulence was generated due to hydrogen development. Changes were more significant after treating MgPawith potassium fluoride. In order to complement MM observations, membrane damage as detected by a lactase dehydrogenase (LDH) assay and mitochondrial activity as detected by a WST-1 assay with macrophages and osteoblasts (MC3T3-E1) were compared. A more significant concentration-dependent effect was detected formacrophages exposed to MgPa than for osteoblasts. Accordingly, complementary data showed that viability and cell cycle seem to be more altered in macrophages. In addition, protein profiles and expression of proteinsassociated with the adhesion process changed in the presence of MgPa. These studies revealed that time-lapseMM is a helpful tool for monitoring changes of biodegradable materials and the biological surrounding in real time andin situ. This information is useful in studies related to biodegradable biomaterials.","palabras_clave":"BIOMATERIAL|MAGNESIUM|BIODEGRADATION|CYTOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4636,"idioma_id":4,"titulo":"The effects of trans fatty acids on triacylglycerol regulation in mice depend on dietary unsaturated fatty acids","resumen":"The aim of this study was to investigate the effects of trans-fatty acids (TFA) on liver and serum TAG regulation in mice fed diets containing different proportions of n-3, n-6 and n-9 unsaturated fatty acids (UFA) from olive (O), maize (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) one of these diets. The effects of the partial substitution (1 %, w\/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and fatty acids oxidation were evaluated, as well as their transcription factor expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL rich in TAG (VLDL-TAG) secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot andRt groups, and this effect was associated with an imbalance between lipogenesis and &#946;-oxidation. In the Ot group, exacerbated lipogenesis may be one of the mechanisms responsible for the liver steatosis induced by TFA, whereas in Rt it has been related to a decreased &#946;-oxidation, compared with their respective controls. The enhanced hepatic VLDL-TAG secretion in the Ot and Rt groups was compensated with a differential removal of TAG by LPL enzyme in extrahepatic tissues, leading to unchanged serum TAG levels. In brief, the effects of low levels of TFA on liver and serum TAG regulation in mice depend on the dietary proportions of n-3, n-6 and n-9 UFA.","palabras_clave":"DIETARY FAT|TRIACYLGLYCEROL METABOLISM|LIPOGENESIS|&#914;-OXIDATION|LIPOPROTEIN LIPASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4638,"idioma_id":4,"titulo":"The Tc TASV proteins are novel promising antigens to detect active Trypanosoma cruzi infection in dogs","resumen":"In regions where Chagas disease is endemic, canine Trypanosoma cruzi infection is highly correlated with the risk of transmission of the parasite to humans. Herein we evaluated the novel TcTASV protein family (subfamilies A, B, C), differentially expressed in bloodstream trypomastigotes, for the detection of naturally infected dogs. A gene of each TcTASV subfamily was cloned and expressed. Indirect enzyme-linked immunosorbent assays (ELISA) were developed using recombinant antigens individually or mixed together. Our results showed that dogs with active T. cruzi infection differentially reacted against the TcTASV-C subfamily. The use of both TcTASV-C plus TcTASV-A proteins (Mix A+C-ELISA) enhanced the reactivity of sera from dogs with active infection, detecting 94% of the evaluated samples. These findings agree with our previous observations, where the infected animals exhibited a quick anti-TcTASV-C antibody response, coincident with the beginning of parasitaemia, in a murine model of the disease. Results obtained in the present work prove that the Mix A+C-ELISA is a specific, simple and cheap technique to be applied in endemic areas in screening studies. The Mix A+C-ELISA could help to differentially detect canine hosts with active infection and therefore with high impact in the risk of transmission to humans.","palabras_clave":"TcTASV|Trypanosoma cruzi|Antígenos|Diagnóstico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4639,"idioma_id":4,"titulo":"Photosynthetic performance associated with phosphorus availability in mats of Didymosphenia geminata (Bacillariophyceae) from Patagonia (Argentina and Chile)","resumen":"Recently reported blooms of Didymosphenia geminata from rivers in Argentinean and Chilean Patagonia, together with non-typical blooms reported from lake systems, have the potential to alter community and ecosystem dynamics of aquatic environments in the region. In an effort to quantify key aspects of ecosystem function of D. geminata mats in Patagonia, we describe for the first time photosynthesis?irradiance (P-E) curves of this species, associated P-E parameters (R, Pmax and a) and their relationship to other functional (alkaline phosphatase activity [APA] enzyme activity and chlorophyll a) and environmental variables (soluble reactive and organic phosphorous, electrical conductivity). Mats of D. geminata from lakes and rivers representing east and west sides of the Andes from 408S to 468S (i.e. much of current known distribution) varied mostly in the a and Pmax parameters of P-E curves. Southern systems showed a higher a and lower Pmax parameters than more northern sites on the east slope of the cordillera. None of the P-E curves showed photoinhibition. P-E parameters for the two lakes fell within the range of values for riverine mats; although, soluble reactive P was notably higher in the lakes. We used two different statistical approaches to evaluate associations across environmental, functional or P-E parameters, finding a strong relation between APA andPmax and a weak one between soluble reactive phosphorus, pH, dissolved organic Phosphorus and functional parameters.Our results do not contradict previous observations on the importance of P availability in communities of D. geminata; although the variability in response across P-E parameters suggests a more complex mechanism of regulation than implied in the existing literature.","palabras_clave":"Diatoms|Invasive species|Phosphorus| Photosynthesisirradiance curves"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4640,"idioma_id":4,"titulo":"Stratifi cation and residence time as factors controlling the seasonal variation and the vertical distribution of chlorophyll-a in a subtropical irrigation reservoir","resumen":"Reservoir trophic state is controlled by light and nutrient availability, as well as by hydraulic management and stratification pattern. In arid zone reservoirs, the inflow and outflow discharges have extreme seasonal variations which produce well-defined cycles of filling and draining. Moreover, since stratification often occurs, epilimnion and hypolimnion renewal rates may vary producing different environmental conditions throughout the water column. These variation patterns may affect phytoplankton growth at both temporal and spatial scales. For twohydrological years, we analyzed the influence of light climate, nutrients, residence time (Tw), and stratification on phytoplankton biomass (as chlorophyll-a [Chl-a]) in an irrigation reservoir (276 hm 3 ) in the arid central Andes in Argentina (33°S). The reservoir was turbid (Z Secchi : 1.4 m) with relatively high levels of dissolved nitrogen and phosphorus (60 mgP L À1 and 560 mgN L À1 , respectively). Stratification occurred from mid-spring to late-summer, when hypolimnetic oxygen and pH decreased whereas dissolved nutrients increased. The reservoir wasmesotrophic (Chl-a: 4.6?10.8 mg L À1 ) and showed two semi-annual peaks of Chl-a (summer and winter). Depending on the water column circulation, Chl-a was directly related to Tw for 60 &gt; Tw &gt; 200 and inversely to ammonia, during mixing and stratification periods,&nbsp; respectively.Phytoplankton development was strongly influenced by the seasonal variations of inflow\/outflow and stratification. Chl-a peaked in summer, when inflow plunged into the hypolimnion of the stratified reservoir and in the warm and mixed epilimnion algae proliferated until nutrientsdepletion. Alternatively, the winter Chl-a maximum is likely to be produced by the higher concentration of nutrients combined with the turbulent mixing in the water column which acts as a mechanism that helps to overcome light-limitation. Since stratification modifies the vertical distribution of Tw, the use of a single annual value of this hydraulic parameter to assess its effect on the reservoir trophic state results inadequate. This paper constitutes the first description ofhydrologic effects on Chl-a dynamics in a reservoir in arid central western Argentina.","palabras_clave":"Nutrients| Reservoir|Stratification |Trophic state"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4641,"idioma_id":4,"titulo":"?In vitro and in vivo effects of ulipristal acetate on fertilization and early embryo  development in mice","resumen":"STUDY QUESTION:Does ulipristal acetate (UPA), a selective progesterone receptor modulator used for emergency contraception (EC), interfere with fertilization or early embryo development in vitro and in vivo?SUMMARY ANSWER:At doses similar to those used for EC, UPA does not affect mouse gamete transport, fertilization or embryo development.WHAT IS KNOWN ALREADY:UPA acts as an emergency contraceptive mainly by inhibiting or delaying ovulation. However, there is little information regarding its effects on post-ovulatory events preceding implantation.STUDY DESIGN, SIZE, DURATION:This was an in vitro and in vivo experimental study involving the use of mouse gametes and embryos from at least three animals in each set of experiments.PARTICIPANTS\/MATERIALS, SETTING, METHODS:For in vitro fertilization experiments, mouse epididymal spermatozoa capacitated in the presence of different concentrations of UPA (0-1000 ng\/ml) were used to inseminate cumulus-intact or cumulus-free eggs in the presence or absence of UPA during gamete co-incubation, and the percentage of fertilized eggs was determined. For in vivo fertilization experiments, superovulated females caged with proven fertile males were injected with UPA (40 mg\/kg) or vehicle just before or just after mating and the percentage of fertilized eggs recovered from the ampulla was determined. To investigate the effect of UPA on embryo development, zygotes were recovered from mated females, cultured in the presence of UPA (1000 ng\/ml) for 4 days and the progression of embryo development was monitored daily.MAIN RESULTS AND THE ROLE OF CHANCE:In vitro studies revealed that the presence of UPA during capacitation and\/or gamete co-incubation does not affect fertilization. Whereas the in vivo administration of UPA at the same time as hCG injection produced a decrease in the number of eggs ovulated compared with controls (vehicle injected animals, P < 0.05), no effects on fertilization were observed when UPA was administered shortly before or after mating. No differences were observed in either the percentage of cleaved embryos or the cleavage speed when UPA was present during in vitro embryo culture.LIMITATIONS, REASONS FOR CAUTION:Considering the ethical and technical limitations inherent to the use of human gametes for fertilization studies, the mouse model was used as an approach for exploring the potential effects of UPA on in vivo sperm transport and fertilization. Nevertheless, the extrapolation of these results to humans requires further investigation.WIDER IMPLICATIONS OF THE FINDINGS:This study presents new evidence on the lack of effect of UPA on gamete interaction and embryo development, providing new insights into the mechanism of action of UPA as an emergency contraceptive method with potential clinical implications. These new findings could contribute to increase the acceptability and proper use of UPA as an emergency contraceptive method.STUDY FUNDING\/COMPETING INTERESTS:This study was partially supported by a National Agency of Scientific and Technological Promotion (ANPCyT), Argentina grants PICT 2011-061 to D.J.C. and PICT 2011-2023 to P.S.C. None of the authors has any competing interests to declare.","palabras_clave":"Emergency contraception|Ulipristal Acetate|Sperm|Fertilization|Embryo development"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4642,"idioma_id":4,"titulo":"Misalignment detection in induction motors with flexible coupling by means of estimated torque analysis and MCSA","resumen":"In recent years, progress has been made in developing techniques to detect mechanicalfaults in actuators driven by induction motors. The latest developments show their cap-ability to detect faults from the analysis of the motor electrical variables. The techniquesare based on the analysis of the Motor Current Signature Analysis (MCSA) and the LoadTorque Signature Analysis (LTSA), among others. Thus, failures such as misalignment be-tween the motor and load, progressive gear teeth wear, and mass imbalances have beensuccessfully detected. In case of misalignment between the motor and load, both angularand radial misalignment, the results presented in literature do not consider the char-acteristics of the coupling device. In this work, it is studied a mechanism in which thepower transmission between the motor and load is performed by means of different typesof couplings, mainly those most frequently used in industry. Results show that the con-clusions drawn for a particular coupling are not necessarily applicable to others. Finally,this paper presents data of interest for the development of algorithms or expert systemsfor fault detection and diagnosis","palabras_clave":"Misalignment |Fault detection|Induction motors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4643,"idioma_id":4,"titulo":"Azospirillum brasilense improves in vitro and ex vitro rooting-acclimatization of jojoba","resumen":"Simmondsia chinensis (jojoba) is an important industrial plant of arid regions generally propagated by seeds or cuttings. Seed propagation has the problem that jojoba is a dioecious plant and sex determination can only be done in plants 3?4 years old. Although in vitro and ex vitro culture of jojoba was developed using different methodologies, no comparative studies of rooting-acclimatization systems with and without A. brasilense inoculation were performed. Established in vitro shoots were induced to root with 0, 25, 50 or 75 &#956;M indole-3-butyric acid (IBA) for 0, 4, 6 or 8 days in modified Murashige and Skoog medium and then, transferred to auxin free medium for 50 days. Maximum rooting percentage evaluated by response surface analysis resulted in 67% with 60 &#956;M IBA for 7 days in A. brasilense inoculated treatment compared to 33% using 50 &#956;M IBA for 5 days in the control treatment. In vitro shoots induced with 50 &#956;M IBA for 6 days and ex vitro shoots induced with 29.5 mM IBA for 20 s were transferred to peat-perlite substrate. Nodal segments obtained from field grown plants were used as explant for ex vitro rooting. Shoots of all treatments were acclimatized in a plug tray containing a mix of sterile peat and perlite and exposed to a regime of gradually decreasing humidity. Some shoots of each treatment were inoculated with A. brasilense Az39 (1.5 × 108 UFC mL&#8722;1) at time of transplant and non-inoculated shoots were the controls. In vitro material showed significantly higher (p &#8804; 0.05) rooting percentage in both substrate and in vitro (34% and 37%, respectively) respect to ex vitro source material (9%). A. brasilense Az39 inoculation increased from 33% to 410% the rooting percentage in the different rooting systems. Acclimatization survival rate in plantlets from in vitro rooting system was 63% whereas in plantlets from ex vitro rooting system was 95%. Inoculated treatments significantly decreased jojoba mesophyll thickness and increased cell density compared to uninoculated treatments. Shoots rooted ex vitro showed higher percentage of tannin cells than those rooted in vitro. Foliar epidermis, stomatal density and stomatal index significantly increased in jojoba plants rooted in substrate compared to in vitro treatments. Replacing in vitro by ex vitro rhizogenesis allows simultaneouly to perform rooting and acclimatization which reduces costs of micropropagation and adds efficiency to plant production. In addition, the in vitro plant source allows to reduce roguing and seasonal depence. A. brasilense Az39 increased rooting-acclimatization final yields and produced anatomical features similar to ex vitro plants. So, ex vitro rooting using in vitro plant source and A. brasilense inoculation is recommended.","palabras_clave":"ACCLIMATIZATION\t|LEAF ANATOMY|RHIZOBACTERIA|RHIZOGENESIS|SIMMONDSIA CHINENSIS|STOMATA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4644,"idioma_id":4,"titulo":"Magnetic behavior of iron modified MCM-41 correlated with clustering processes from the wet impregnation method.","resumen":"Magnetic MCM-41 type mesoporous silica materials were synthetized and modified with different iron loadings by the wet impregnation method. The evolution of iron speciation, depending on the metal loading and associated with a particular magnetic behavior was investigated by M vs. H curves, FC-ZFC curves, EPR spectroscopy and other complementary techniques such as SEM, TEM, and chemisorption of pyridine followed by FT-IR studies. A superparamagnetic contribution was larger for the lower loadings suggesting the high dispersion of very small sized iron nanospecies. However, this contribution decreased with increasing metal loading due to the growth of magnetically blocked nanoparticles (hematite) on the outer surface. Finally, a bimodal size distribution for the superparamagnetic nanospecies could be inferred; then the anisotropy constant for this phase and the corresponding nanospecies sizes were estimated.","palabras_clave":"Fe MODIFIED MCM-41|WET IMPREGNATION|SUPERPARAMAGNETISM|ZFC-FC CURVES|BLOCKING TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4645,"idioma_id":5,"titulo":"Llueve, pero hay ?alegría? en la Ciudad: Retrato del acto de lanzamiento de la campaña electoral 2013 de la juventud del Pro de la Ciudad Autónoma de Buenos Aires","resumen":"Este trabajo analiza las experiencias de politicidad en la militancia juvenil del partido Propuesta Republicana (PRO) en la Ciudad Autónoma de Buenos Aires. A partir de cruces entre ciencia política, antropología, sociología política y estudios de juventudes, se busca aportar conocimientos acerca de las formas de participación política juvenil en la Argentina contemporánea. Primero se presenta una mirada por los estudios sobre juventudes y política, para luego abordar la caracterización del espacio juvenil del PRO en la CABA. En segundo lugar, se analizan las experiencias de politicidad en base a registros del acto de lanzamiento de la juventud del PRO durante la campaña de 2013, a partir de los cuales se da cuenta tanto de la imagen juvenil que el partido busca representar, así como de dimensiones de sociabilidad que muestran cierta horizontalidad de las relaciones entre los militantes, que contrastan con la forma que toman los posicionamientos internos.","palabras_clave":"Juventudes|Participación Política|Partidos Políticos|Campaña Electoral|Politicidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4646,"idioma_id":4,"titulo":"Field Capture, Chemical Immobilization, and Morphometrics of a Little-Studied South American Carnivore, the Lesser Grison","resumen":"A total of 23 free-ranging lesser grisons (Galictis cuja) were live trapped and 9 were radio-marked in two areas of the Pampas grasslands of Argentina. We assessed the chemical immobilization and success of trapping devices on this little-known South American mustelid. Both box-traps and rubber-padded leg-hold traps allowed us to capture lesser grisons. However, leg-hold traps had greater capture efficiency. Although the liberation time was longer with tiletamine-zolazepam than ketamine-xylazine, field immobilization of lesser grisons with both drug combinations was effective and safe. The high frequency of thermal regulation problems suggests that body temperatures of chemically-restrained lesser grisons should be routinely monitored. Neither collars nor backpack harnesses were effective methods for attachment of radio transmitters. Trappedindividuals showed male-skewed sexual size dimorphism and sex ratio of trappedindividuals. Capture times suggest that lesser grisons are largely diurnal. Most of the data presented in this study are the first published on the lesser grison and provide baseline information for future research and conservation.","palabras_clave":"ANESTHESIA|BOX-TRAP|CAPTURE RATE|GRASSLANDS|LEG-HOLD TRAP|MUSTELIDAE|RADIOTELEMETRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4647,"idioma_id":4,"titulo":"Biodegradable PLA\/PBAT\/Clay Nanocomposites: Morphological, Rheological and Thermomechanical Behavior","resumen":"Poly(lactic acid)\/poly(butylene adipate-co-terephthalate) (PLA\/PBAT)-based nanocomposites were prepared by melt blending of PLA and PBAT with 5 wt% of unmodified (Cloisite Na) and modified (Cloisite 30B) montmorillonites. X-ray diffraction (XRD) revealed an intercalated structure in both nanocomposites. Theextent of the intercalation was higher for nanocomposites based on modified clays (OMMT) with chemical affinity with the polymer matrix. Rheological measurements have shown an increase in viscosity and a better degree of clay dispersion for nanocomposites containing OMMT. Nanocomposites with OMMT showed lower PBAT separated phase particle size and improvements in thermal stability, mechanical properties and water vapor barrier when compared with the neat blend. Finally, our results showed that the organically modified clay has a higher affinity than natural clay with the studied polymer blend.","palabras_clave":"PLA PBAT|NANOCOMPOSITES|CLAYS|MONTMORILLONITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4648,"idioma_id":5,"titulo":"Registro de cultivar Yavú-UNRC","resumen":"Yavú-UNRC es un cultivar de Triticale (X Triticosecale Wittmack)2n=6x=42, inscripto en el Registro Nacional de Cultivares del Instituto Nacional de Semillas. Expte. INASE Nº 15.191, Resolución INASE Nº 135\/2014 del 04\/06\/2014. Fue obtenido en el Criadero A\/1739 UNINARC, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto. Deriva del cruzamiento original CIMMYT SVHT 02\/BORA\/3\/YOGUI_1\/TARASCA 87_3\/ \/HARE_212. Fue seleccionado para uso forrajero de doble propósito (pasto y grano) a partir de una introducción recibida en F4 formando parte del FWTCL 1995 (Ensayo Internacional de Triticales Forrajeros de Invierno) del CIMMYT. Yavú-UNRC tiene hábito de crecimiento intermedio-largo con porte juvenil semierecto, macollaje abundante y vigoroso crecimiento durante todo el ciclo vegetativo. Ciclo emergencia a floración plena 128 ± 6 días y de 165 ± 5 días a madurez de cosecha en siembras para grano. Ciclo vegetativo similar a Genú-UNRC, más largo que Tizné-UNRC y algo más corto que Quiñé-UNRC. Sensible al fotoperíodo, con moderados requerimientos de frío. La altura de planta a la madurez = 94 ± 17 cm según año y disponibilidad de agua durante el ciclo. Es significativamente más bajo que los testigos Genú y Tizné-UNRC. Cuello ondulado poco piloso; espiga inclinada como Tizné-UNRC y de forma oblonga como Cayú-UNRC.","palabras_clave":"Cultivar|Doble propósito|Triticale"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4649,"idioma_id":4,"titulo":"An ostracod-based calibration function for electrical conductivity reconstruction in lacustrine environments in Patagonia, Southern South America","resumen":"In the Patagonian region (&#8764;37?56°S) E of the Andes, the salinity and solute composition of lakes is strongly related to their location along the marked W-E decreasing precipitation gradient that is one of the main climatic features of the area. A calibration function (n&nbsp;=&nbsp;34) based on 12 ostracod species (Ostracoda, Crustacea) was developed by WA-PLS to quantitatively reconstruct electrical conductivity (EC) values as a salinity proxy. The selected one component model had a r2&nbsp;=&nbsp;0.74 and RMSEP and maximum bias equal to 16% and 31% of the sampled range, respectively, comparable to other published ostracod-based calibration functions. This model was applied to the ostracod record of the closed lake Laguna Cháltel (49°58&#8242;S, 71°07&#8242;W), comprising seven species and dominated by two species of the genus Limnocythere. In order to evaluate the calibration function?s robustness, the obtained EC values were compared with qualitative lake level and salinity variations inferred through a multiproxy hydrological reconstruction of the lake. Both reconstructions show good overall agreement, with reconstructed EC values in the oligo-mesohaline range (average: 11 060&nbsp;±&nbsp;680&nbsp;&#956;S\/cm) between 4570 and 3190&nbsp;cal BP, corresponding to the ephemeral and shallow lake phases, and a marked decrease in EC concurrent with a lake level rise, reaching an average EC of 1140&nbsp;±&nbsp;90&nbsp;&#956;S\/cm during the deep lake phase (1720&nbsp;cal BP to present). The variability in the reconstructed EC values for the ephemeral lake phase showed some inconsistency with the expected trend, which was attributed to time-averaging effects; for its part, the pace of the decrease in EC during the medium-depth phase (3190?1720&nbsp;cal BP) differed from the expected, which could be due to autigenic effects (redissolution of salts) at the onset of this phase. This comparison not only lends support to the adequacy of the calibration function, but also suggests that its application in the context of a multiproxy study can greatly contribute to distinguish between autigenic and climatic-related controls of paleosalinity in closed lakes, allowing performing more accurate paleoenvironmental inferences on the basis of paleohydrological reconstructions.","palabras_clave":"Quantitative salinity reconstruction|Transfer function|Holocene|Ostracoda|Paleohydrology|Closed lakes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4650,"idioma_id":4,"titulo":"Methodology for classification of geographical features with remote sensing images: Application to tidal flats","resumen":"Tidal flats generally exhibit ponds of diverse size, shape,orientation and origin. Studying the genesis, evolution, stability anderosive mechanisms of these geographic features is critical to understandthe dynamics of coastal wetlands. However, monitoring these locationsthrough direct access is hard and expensive, not always feasible, andenvironmentally damaging. Processing remote sensing images is a naturalalternative for the extraction of qualitative and quantitative data dueto their noninvasive nature. In this work, a robust methodology forautomatic classification of ponds and tidal creeks in tidal flats usingGoogle Earth images is proposed. The applicability of our method istested in nine zones with different morphological settings. Each zone isprocessed by a segmentation stage, where ponds and tidal creeks areidentified. Next, each geographical feature is measured and a set ofshape descriptors is calculated. This dataset, together with a-prioriclassification of each geographical feature, is used to define aregression model, which allows a massive automatic classification oflarge volumes of data discriminating ponds and tidal creeks against otherspurious geographical features. In all cases, we identified andautomatically classified different geographic features with an averageaccuracy over 90% (89.7% in the worst case, and 99.4% in the best case).These results show the feasibility of using freely available Google Earthimagery for the automatic identification and classification of complexgeographical features, as ponds and tidal creeks. Also, the presentedmethodology may be easily applied in other wetlands of the world and evenemploying any other kind of remote sensing imagery.","palabras_clave":"PONDS|TIDAL COURSES|OBJECT DETECTION|SHAPE DESCRIPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4651,"idioma_id":4,"titulo":"Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis","resumen":"The aim of this study was to detect, isolate and genetically characterize Toxoplasma gondii from tissues obtained from free range chickens which were breed in farms from patients with toxoplasmic retinochoroiditis in Misiones, Argentina. Thirty three samples of head (refrigerated=18 and frozen=15) from free range chickens were processed.Refrigerated (n=18) chicken central nervous systems (CNS) were bioassay in mice.DNA was obtained from all samples (n=33) and PCR was performed using TOX5 TOX8 T. gondii specific primers. Positive PCR samples were characterized by nested PCR and restriction fragment length polymorphism using the markers SAG2, BTUB,GRA6, SAG3, PK1, L358, C22-8, C29-2 and Apico. T. gondii DNA was amplified in 30.3 % (10\/33) of CNS samples. Isolates were obtained in 27.7% (5\/18) of inoculated CNS samples (TgCk11-9Arg, TgCk13-5Arg, TgCk14-5Arg, TgCk14-6Arg and TgCk14-7Arg). Seven samples showed a restriction pattern to all markers and were identified as atypical with several alleles type III. Genotyping of T. gondii from samples of patients with retinochoroiditis in the same area could improve the understanding of the epidemiology of toxoplasmosis in the region.","palabras_clave":"Toxoplasma gondii|free-range chickens|retichoroiditis|isolates|genotyping"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4652,"idioma_id":10,"titulo":"Sarcocystis masoni, n. sp. (Apicomplexa: Sarcocystidae), and redescription of Sarcocystis aucheniae from llama (Lama glama), guanaco (Lama guanicoe) and alpaca (Vicugna pacos)","resumen":"There is considerable confusion concerning the species of Sarcocystis in South American camelids (SAC). Several speciesnames have been used; however, proper descriptions are lacking. In the present paper, we redescribe the macroscopic sarcocyst forming Sarcocystis aucheniae and describe and propose a new name, Sarcocystis masoni for the microscopic sarcocyst forming species. Muscles samples were obtained from llamas (Lama glama) and guanacos (Lama guanicoe) fromArgentina and from alpacas (Vicugna pacos) and llamas from Peru. Individual sarcocysts were processed by optical andelectron microscopy, and molecular studies. Microscopic sarcocysts of S. masoni were up to 800 µm long and 35?95 µmwide, the sarcocyst wall was 2·5?3·5 µm thick, and had conical to cylindrical villar protrusions (vp) with several microtubules. Each vp had 11 or more rows of knob-like projections. Seven 18S rRNA gene sequences obtained from sarcocystsrevealed 95?96% identity with other Sarcocystis spp. sequences reported in the GenBank. Sarcocysts of S. aucheniae weremacroscopic, up to 1·2 cm long and surrounded by a dense and laminar 50 µm thick secondary cyst wall. The sarcocyst wallwas up to 10 µm thick, and had branched vp, appearing like cauliflower. Comparison of the 11 sequences obtained fromindividual macroscopic cysts evidenced a 98?99% of sequence homology with other S. aucheniae sequences. In conclusion,2 morphologically and molecularly different Sarcocystis species, S. masoni (microscopic cysts) and S. aucheniae (macroscopic cysts), were identified affecting different SAC from Argentina and Peru.","palabras_clave":"SARCOCYSTIS SPP. |SOUTH AMERICAN CAMELIDS|PCR|SEQUENCING|ELECTRON MICROSPY|ARGENTINA|PERU"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4653,"idioma_id":4,"titulo":"Determination of alkaloids in onion nectar by micellar electrokinetic chromatography","resumen":"Nectar is the most important floral reward offered by plants to insects.Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. Considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar byMEKC-UV is herein reported. The extraction of alkaloid compounds in nectar was performed by SPE using a homemade miniaturized column (C18). Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire optimum conditions. Under the proposed conditions, the analytes can be separated within 15 min in a 50 cm effective length capillary (75 m id) at a separation voltage of 20 kV in 20 mmol\/L sodium tretraborate, 100 mmol\/L SDS. The amount of sample requirement was reduced up to 2000 times, when compared to traditional methods, reaching limits of detection as low as 0.0153 ng\/L. For the first time, this study demonstrates that there are marked qualitative and quantitative differences in nectar alkaloids between open pollinated and male sterile lines (MSLs) and also within MSLs.","palabras_clave":"Allium cepa|Male sterility|Nectar|Seed yield"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4654,"idioma_id":4,"titulo":". Phylogenetic relationships among species of the Neotropical genus Graomys (Rodentia, Cricetidae): contrasting patterns of skull morphometric variation and genetic divergence.","resumen":"Subtle external traits differentiation characterizes the rodents of the Neotropical genus Graomys, whereas marked differences in karyotype number were reported among some species. We evaluate here the possible evolutionary forces involved in the evolution of the genus by assessing the degree of intra- and interspecific genetic and morphological variation. The phylogenetic analysis demonstrates the existence of at least three species with high levels of genetic distance (10%), diverged between 1-1.5 million years ago. Neither G. griseoflavus nor G. chacoensis presents marked phylogeographic structure. Regarding morphological characters, these species show shape differences in the skull, which could be attributable to differences in the ecoregions they inhabit, being more marked in G. griseoflavus than in G. chacoensis. The skull shape of G. chacoensis could have evolved under genetic drift, whereas evidence here presented indicates that this character could be under selective pressures in G. griseoflavus. Reconstruction of the ancestral area suggests that G. griseoflavus originated in the central Monte desert and G. chacoensis in the Chaco ecoregion surrounding the austral extreme of the Yungas rainforest; later both species would have undergone demographic and geographic expansions nearly simultaneously, starting around 150,000 - 175,000 years ago. The complex evolutionary history of the genus could, at least in part, be explained by the decoupling of morphological, karyological and molecular characters.","palabras_clave":"Molecular systematic|Phylogeography|Sigmodontinae|Skull geometric morphometrics|Intraspecific variation|Interspecific variation|Species boundaries"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4655,"idioma_id":4,"titulo":"Multivalent sialylation of beta-thio-glycoclusters by Trypanosoma cruzi trans sialidase and analysis by High Performance Anion Exchange Chromatography","resumen":"The synthesis of multivalent sialylated glycoclusters is herein addressed by a chemoenzymatic approach using the trans-sialidase of Trypanosoma cruzi (TcTS). Multivalent &#946;-thio-galactopyranosides and &#946;-thio-lactosides were used as acceptor substrates and 3&#8242;-sialyllactose as the sialic acid donor. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was shown to be an excellent technique for the analysis of the reaction products. Different eluting conditions were optimized to allow the simultaneous resolution of the sialylated species, as well as their neutral precursors. The TcTS efficiently transferred sialyl residues to di, tri, tetra and octa &#946;-thiogalactosides. In the case of an octavalent thiolactoside, up to six polysialylated compounds could be resolved. Preparative sialylation reactions were performed using the tetravalent and octavalent acceptor substrates. The main sialylated derivatives could be unequivocally assigned by MALDI mass spectrometry. Inhibition of the transfer to the natural substrate, N-acetyllactosamine, was also studied. The octalactoside caused 82 % inhibition of sialic acid transfer when we used equimolar concentrations of donor, acceptor and inhibitor.","palabras_clave":"T. cruzi trans-sialidase|beta-galactopyranosides|multivalent glycoclusters|sialic acid|HPAEC|MALDI-TOF|enzymatic sialylation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4656,"idioma_id":4,"titulo":"Relationship between microbial functions and community structure in response to agricultural intensification in Northwestern Argentina","resumen":"Intensification of agricultural systems through the use of intensive agriculture and the advance of deforestation have led to a decrease of soil biological quality. Soil functional and structural microbiota are sensitive parameters to monitor changes caused by agricultural use. Different sites under soybean monoculture (continuous soybean) and soybean\/maize rotation practices were selected. Samples were collected from agricultural soils under different periods of implantation: a 4-year rotation (R4), a 15-year rotation (R15), a 5-year monoculture (M5), and a 24-year monoculture (M24). A site of native vegetation under recently agricultural production (RUA) was also sampled. Native vegetation soil (NV) adjacent to agricultural sites were sampled as a control. In general, the results showed that RUA and M24 had lower enzyme activities, less microbial abundance and low physical and chemical soil quality than those subjected to crop rotation. In contrast, both the bacterial and total microbial biomasses were significantly higher in NV and crop rotation than in soils under monoculture systems. Although we expected that differences in microbial activities would be due to changes in microbial community abundance, our results indicated that changes in soil management produced faster alterations to soil enzyme activities than any modifications induced in the microbial community structure. Consequently, both aspects of microbial diversity, namely function and structure, were affected independently by agricultural intensification.","palabras_clave":"soil |monoculture|rotation|microbial diversity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4657,"idioma_id":5,"titulo":"Estudiantes hoy, entre Facebook, Google y Metacognición. Ideas para innovar en la Educación Superior","resumen":"En los estudios superiores, al igual que en los demás contextos educativos, no basta con adquirir una serie de conocimientos, es indispensable reflexionar sobre los propios procesos cognitivos para tomar decisiones autónomas y fundamentadas respecto de los aprendizajes. Aprender va mucho más allá de las cuestiones cognitivas, las emociones puestas en juego y las posibilidades de reflexionar respecto de los aprendizajes resultan decisivas en los complejos procesos de construcción de conocimientos. El objetivo del estudio es analizar aspectos metacognitivos vinculados al aprendizaje académico de estudiantes de primer año del Profesorado en Educación Inicial de la Universidad Nacional de Río Cuarto y del Instituto Superior María Inmaculada (Argentina). En la primera parte de la investigación, los estudiantes respondieron a un cuestionario general referido a prácticas y hábitos de estudio y a un inventario de patrones de aprendizaje: ILS (Vermunt, 1998). Luego, se realizaron entrevistas grupales con los estudiantes con el propósito de conocer diferentes puntos de vista respecto de las prácticas de estudio, aprendizaje y enseñanza en la educación superior. Los resultados muestran que la mayoría de los participantes requieren de regulaciones externas para desarrollar los procesos de aprendizaje. Se observan ciertas dificultades en la autorregulación de los procesos de lectura, escritura y en el estudio en general. Se propone desarrollar estrategias innovadoras que promuevan en los estudiantes prácticas reflexivas y metacognitivas respecto de los aprendizajes en contextos académicos.","palabras_clave":"METACOGNICIÓN|EMOCIONES|APRENDIZAJE|VIRTUALIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4658,"idioma_id":4,"titulo":"Handshap e recognition for Argentinian Sign Language using ProbSom","resumen":"Automatic sign language recognition is an important topic within the areas of human-computer interaction and machine learning.&nbsp; On the one hand, it poses a complex challenge that requires the intervention of various knowledge areas, such as video processing, image processing, intelligent systems and linguistics. On the other hand, robust recognition of sign language could assist in the translation process and the integration of hearing-impaired people. This paper offers two main contributions: first, the creation of a database of handshapes for the Argentinian Sign Language (LSA), which is a topic that has barely been discussed so far. Secondly, a technique for image processing,&nbsp; feature extraction and subsequent handshape classification using a supervised adaptation of self-organizing maps that is called ProbSom. This technique is compared to others in the state of the art, such as Support Vector Machines (SVM), Random Forests, and Neural Networks. The database that was built contains 800 images with 16 LSA conjurations, and is a first step towards building a comprehensive database of Argentinian signs. The ProbSom-based neural classifier, using the proposed descriptor, achieved an accuracy rate above 90.","palabras_clave":"HANDSHAPE RECOGNITION|SIGN LANGUAGE RECOGNITION|SOM|RADON TRANSFORM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4659,"idioma_id":4,"titulo":"Use effects on the reflection of macro textured surfaces","resumen":"The emergence of led luminaries in street lighting has modified traditional design guidelines.  The highest led energy efficiency allows keeping suitable lighting levels with less installed power. Thus, the present design line of a street lighting installation is closely related to the technological change: kind of led to be used, suitable lens, efficacy relationship regarding high pressure sodium, light colour temperature, spectrum.  The ?impact? associated to with led conversion relegates main aspects of street designs: effect of road reflective properties and installation depreciation. The wear produced by traffic, as well as the environmental conditions gradually modifies the road reflection matrix. In a street lighting installation, this change alters the luminance arrangement on the road together with the perception conditions. This phenomenon is a determining factor within the global concept of depreciation affecting lighting systems in general and also street lighting installations designed by the Luminance Technique.  In the present work changes, due to use, in the shape of street reflection matrices of Argentinean highways are analyzed. The analyzed r-tables were obtained through ?in-situ? measurements, with road reflectometer. A first group corresponds to the initial conditions of macro textured roads which were measured in the period 2001\/2003. The second studied set contains r-tables of the same type, and according to its use the surfaces can be considered in steady state, assessed between 2005 and 2009. The study provides information about the shape of matrixes and its alteration, specularity and scale factor, relating such parameters with the possible effects on the resulting lighting.","palabras_clave":"LED|LIGHTING|ROAD|SURFACE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4660,"idioma_id":4,"titulo":"Drivers of highly diverse planktonic ciliate  assemblages in peat bog pools from Tierra del Fuego (Argentina).","resumen":"Peatlands often encompass shallow pools, wherein dystrophic and colored acid waters host a remarkably diverse biota, with ciliates likely playing a key role in their short trophic webs. In the Sphagnum magellanicum-dominated Rancho Hambre peat bog, a 2-year study was conducted in five pools with different morphometric and trophic characteristics, in order to identify main environmental variables driving ciliate species richness, abundance, biomass, and diversity. Overall species richness (125 taxa) was much higher than in northern Hemisphere counterparts. Deep minerotrophic pools hosted the richest communities, showing similar seasonal abundance patterns and the highest species turnover. Although all pools shared the same dominant ciliates, similarity in taxonomic composition among them was generally low (J = 0.22?0.35). Moreover, IndVal analysis showed that rare and occasional species were highly indicative of different pools. Euryoecious, heterotrophic species, occurred in all sites, while mixotrophs were typical from shallow ombrotrophic pools. Rimostrombidium hyalinum was the most indicative species of a deep ombrotrophic pool. A CCA revealed that the abundances of potential ciliate preys, i.e., picophytoplankton, bacterioplankton, and heterotrophic flagellates, were the most significant regulators of abundances of this group. Therefore, ciliate structure and dynamics were influenced by pool morphometry and physicaland chemical features, but foremost by interactions with other plankton communities.","palabras_clave":"PLANKTONIC CILIATES|DIVERSITY|IND VAL|PEAT BOG POOLS|TIERRA DEL FUEGO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4661,"idioma_id":4,"titulo":"Nitrification and ammonia-oxidizing bacteria shift in response to soil moisture and plant litter quality in arid soils from the Patagonian Monte","resumen":"We aimed to evaluate the effects of both plant litter quality, characteristic of sites with different histories of grazing disturbance, and soil water content on nitrification in soils from an arid ecosystem of Patagonia. To reach this goal, soil microcosms covered by plant litter of different quality and subjected to different soil water conditions were sampled at different times to analyze: (i) the nitrifying enzyme activity; (ii) the concentration of inorganic forms of nitrogen; and (iii) the abundance of bacterial (AOB) and archaeal (AOA) amoA genes. Soil water enhanced nitrifying activity in average 16% during the period of highest nitrification rates, and nitrate concentration in average 733% after 70 days of incubation. Microcosms amended with high litter quality showed the highest ammonium and the lowest phenolics concentrations, and higher or equal nitrification rates than microcosms amended with poor litter quality. After one week of incubation, the combination of both high litter quality and soil water significantly enhanced amoA gene abundance from AOB (p &lt; 0.05). The AOB:AOA amoA genes ratio ranged from 12 to 3,170. Altogether, our results suggest that high soil water and litter quality exerted positive effects over the nitrifying activity and the abundance of AOB but not AOA in these arid soils.","palabras_clave":"NITRIFICATION|ARID SOILS|AMMONIA-OXIDIZING BACTERIA|AMMONIA-OXIDIZING ARCHAEA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4662,"idioma_id":4,"titulo":"The dual role of nitric oxide in guard cells: promoting and attenuating the ABA and phospholipid-derived signals leading to the stomatal closure","resumen":"OVERVIEWOFTHEABA-INDUCEDSIGNALINGLEADINGTOTHEREGULATIONOFSTOMATALMOVEMENTPlants regulatethegasexchangewiththeenvironmentthroughmicroscopicporesformedbyspecializedcellscalledguardcellsthatconstitutethestomata.ThecontrolofwaterlossandCO2 uptakeofplantsreliesonthesizeofthestomatalpore.Abscisicacid(ABA)isthemasterhormone governingtheintricatenetworkofmolecularswitchesandphysiologicalresponsesofguard cellsthatdeterminethedegreeofstomatalaperture.","palabras_clave":"Nitric oxide|Phosphatidic acid|guard cells|stomata"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4663,"idioma_id":4,"titulo":"Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers","resumen":"Bovine mastitis produces economic losses, attributable to the decrease in milk production, reduced milk quality, costs of treatment and replacement of animals. A successful prophylactic vaccine against Staphylococcus aureus should elicit both humoral and cellular immune responses. In a previous report we evaluated the effectiveness of a live vaccine to protect heifers against challenge with a virulent strain. In the present study the immunological response of heifers after combined immunization schedule was investigated. In a first experimental trial, heifers were vaccinated with 3 subcutaneous doses of avirulent mutant S. aureus RC122 before calving and one intramammary dose (IMD) after calving. Antibodies concentration in blood, bactericidal effect of serum from vaccinated animals and lymphocyte proliferation was determined. The levels of total IgG, IgG1 and IgG2 in colostrum and the lymphocyte proliferation index were significantly higher in vaccinated respect to non-vaccinated group throughout the experiment.The second trial, where animals were inoculated with different vaccination schedules, was carriedout to determine the effect of the IMD on the level of antibodies in blood and milk, cytokines (IL-13 and IFN-) concentration and milk?s SCC and bacteriology. The bacterial growth of the S. aureus strains was totally inhibited at 1?3× 106 and 1?3× 103 cfu\/ml, when the strains were mixed with pooled serumdiluted 1\/40. The results shown that IMD has not a significant effect on the features determinate. In conclusion, a vaccination schedule involving three SC doses before calving would be enough to stimulate antibodies production in milk without an IMD. Furthermore, the results showed a bactericidal effect ofserum from vaccinated animals and this provides further evidence about serum functionality. Immune responses, humoral (antigen-specific antibodies and Th2 type cytokines) and cellular (T-lymphocyte proliferation responses and Th1 type cytokines), were augmented by administration of the avirulent mutantwhich represent an antigenic pool.","palabras_clave":"MASTITIS BOVINA|STAPHYLOCOCCUS AUREUS|VACUNA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4664,"idioma_id":4,"titulo":"Fermentation Performance of Selected Microorganisms in Malbec Musts under Two Different Wine Making Conditions","resumen":"This work evaluates the fermentation performance of simultaneous cultures ofselected indigenous Saccharomyces cerevisiae mc2, Kloeckera apiculata mF andOenococcus oeni X2L in Malbec musts. Fermentations (3 L) were carried outunder two conditions: 1-initial pH 5.5, 60.24 mg\/L SO2, 26°C) and 2-initial pH3.8, 125 mg\/L SO2, 28°C), using different culture combivnations and S. cerevisiaeIOC 18 to 2007 as a control. The final wine densities were 0.992 to 0.997 g\/L (10days). Micro-organisms grew in all conditions. Yeasts prevailed, especially S.cerevisiae (108 CFU\/ml), while O. oeni reached a value of 107 CFU\/ml. Totalreducing sugars consumption was >80% and full malate consumption wasobserved in all musts inoculated with O. oeni. Ethanol formation corresponded tothe Malbec variety (310 to 342 mM), while total acidity did not exceed theorganoleptic quality limit (4.6 to 6.49 g\/L). Glycerol production wasapproximately, 84.3 to 95.1 mM. Among the esters tested, ethyl acetate\/2-phenylethyl acetate showed the highest concentration. The sensory analysis showedthe highest scores for visual descriptors (fluency, color intensity and tonality),olfactory intensity, taste descriptors and general harmony. Thus, we proposed aninoculation condition using mixed yeasts-O. oeni culture and an optimalstatistical point to improve the microbial performance in order to obtain qualityMalbec wines, thus, enhancing the body and sensorial properties","palabras_clave":"MALBEC WINE|FERMENTATION|MIXED YEASTS CULTURE|OENOCOCCUS OENI|GLYCEROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4665,"idioma_id":4,"titulo":"Dinoflagellate Cyst Zonation for the middle to upper Eocene in the Austral Basin, Southwestern Atlantic Ocean - Implications for regional and global correlation","resumen":"The well-exposed marine Eocene units from southwestern Patagonia, Argentina hold usefull information to reconstruct regional climate and oceanographic patterns in an area adjacent to the Drake Passage. The aim of this paper is to integrate dinoflagellate cyst data from three sections of the southwestern Austral Basin (Río Turbio Formation) to propose a zonation scheme, which can be applied to other Southwestern Atlantic Ocean sites. The assemblages of organic walled dinoflagellate cysts have been analysed in different cropping out sections and cores showing the high potential of this fossil group as biostratigraphic markers. The comparison of dinoflagellate cyst events of the upper member of Río Turbio Formation with callibrated biostratigraphic ranges in the Paleogene Southern Pacific Ocean allowed us to date and correlate these sedimentary sections. The resulting zonation consists of four dinoflagellate cyst zones labeled RTF 1 to RTF 4, between middle Lutetian to late Priabonian. As a final point, we applied dinoflagellate cyst species with importance as palaeoenvironmental markers to assess long-term climatic and oceanographic evolution for the area. This study shows that the endemic-Antarctic dinoflagellate cyst assemblage are dominant during the middle to late Eocene (RTF 1 to RTF 3), while a significant replacement of these taxa by cosmopolitan species characterizes the upper part of the upper member of the Río Turbio Formation (RTF 4). This turnover seems to be a consequence of the changes in the ocean circulation patterns forced by the deepening of the southern Atlantic gateways (the Dake Passage and the Tasman Gateway). ","palabras_clave":"Biostratigraphy|Palaeoenvironment|Eocene|Austral Basin, Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4666,"idioma_id":4,"titulo":"Electrophoretic deposition of chitosan\/Bioglass® and chitosan\/Bioglass®\/TiO2 composite coatings for bioimplants","resumen":"Electrophoretic deposition (EPD) method has been developed for the fabrication of chitosan\/Bioglass® and chitosan\/Bioglass®\/TiO2 composite coatings on stainless steel substrates for biomedical applications. Microstructure, thickness, and mechanical properties, such as Vickers microhardness, compressive strength, and elastic modulus were determined. The use of chitosan enabled the co-EPD of Bioglass® and TiO2 particles and offered the advantage of room temperature processing typical of EPD. The coating composition was varied by the combination of Bioglass® and TiO2 concentrations in the chitosan solutions used for EPD. The cathodic deposition yield was studied at constant voltage for various deposition times. The coatings were microstructurally characterized by scanning electron microscopy (SEM) and energy dispersive spectroscopy(EDS). Homogeneous chitosan\/Bioglass® coatings on stainless steel substrates were obtained at constant voltage condition using aqueous suspensions based on 0.016 wt% chitosan and 2 wt% Bioglass®. The addition of TiO2 nanopowder to the composite coating was confirmed to improve the hardness, Young´s modulus and compressive strength of the base chitosan\/Bioglass® coating.","palabras_clave":"Electrophoretic deposition|bioimplants|composite coatings"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4667,"idioma_id":4,"titulo":"Combined use of mild heat treatment and refrigeration to extend the postharvest life of organic pepper sticks, as affected by fruit maturity stage","resumen":"Given the proscriptionof using chemicals from synthesis, the alternatives for postharvest managementof organic produce are limited. Consequently, great interest is being devotedto develop and optimize alternative postharvest approaches. In this work we testedmild heat treatments for green and red fresh-cut peppers and evaluated their effecton quality maintenance under normal distribution and retail temperatures (4 °C).Pepper sticks (1 × 5 cm) at red and green ripening stages were heat-treated (HT)by immersion in water at 45, 50 or 55 ºC (1, 3 or 5 minutes) and qualitymaintenance during storage was evaluated. Green peppers were more tolerant toHT than red fruit. Both green and ripe peppers subjected to hot water dips at45 ºC for 3 min showed lower spoilage than the control. The treatments markedlyreduced soft rots (2 and 4 fold for red and green fruit respectively). Hotwater dips also prevented shriveling, weight loss, color changes and contributedto maintain lower fruit respiration during storage. The treatments did not altersugar content, acidity or antioxidant capacity. Despite of the effectivecontrol of soft rots only a slight reduction of microbial counts (&lt;1 log CFUg-1) was found. This suggests that other responses besides biocide effectsor microbial wash-off are involved. The treatments delayed pectin solubilizationand softening and prevented membrane leakage. Short mild heat treatments (45°C, 3 min) may be a simple and appealing non-chemical approach to supplementthe benefits of low temperature management, extending the shelf life of organicfresh-cut green and red peppers. ","palabras_clave":"quality|postharvest|minimally processed|hot water"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4668,"idioma_id":4,"titulo":"Estimation of Live Fuel Moisture Content From MODIS Images for Fire Danger Assessment in Southern Gran Chaco","resumen":"Moisture content oflive fuels (LFMC) is one of the main factors determining fuel flammability and,therefore, a key indicator of fire danger. In this study we modeled therelationship between spectral indices derived from satellite imagery and fieldestimations of LFMC in the Chaco Serrano subregion; then, we analyzed therelationship between fire danger estimations based on LFMC calculations andfire activity. Empirical LFMC models fitted for grasslands, Chaco Serrano forests, and glossy privet forests may beconsidered very accurate (R2 &gt; 0.80), whereas modelscorresponding to shrublands still need to be improved (R2 = 0.57).Monthly maps of fire danger reflected theoccurrence of fires consistently duringyears of both high and low fire activity. Most fires occurred mainly in areaswith high or extreme fire danger, demonstrating a clear relationship betweenLFMC and fire activity in the Chaco Serrano subregion. Our LFMC models may be usefulto assess the spatio-temporaldistribution of fire danger in the Chaco Serrano subregion using remote sensingdata. The associated fire danger maps represent a valuable tool for improving decisionmaking processes to organize early warning and fire suppression activities. ","palabras_clave":"Fires|Risk analysis|Prediction methods|Moisture measurement|Modeling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4669,"idioma_id":4,"titulo":"THE PALEOBOTANICAL RECORD OF THE TRIASSIC CERRO DE LAS CABRAS FORMATION AT ITS TYPE LOCALITY, POTRERILLOS, MENDOZA (USPALLATA GROUP): AN HISTORICAL ACCOUNT AND FIRST RECORD OF FOSSIL FLORA","resumen":"The first fossil macroflora assemblage from the Cerro de Las Cabras Formation at its type locality, in the Potrerillos area, is presented. The area is tectonically altered, which is why there have been several different interpretations about the real thickness and boundaries of the formation. A detailed analysis of the bibliography showed that previous fossil plant taxa referred to the Cerro de Las Cabras Formation belonged to the overlying Potrerillos Formation. However, the recent discovery of a plant-bearing level in stratigraphically constrained sediments of the Cerro de Las Cabras Formation allows the identification of a flora composed of Corystospermales ?Dicroidium odontopteroides (Morris) Gothan; D. lancifolium (Morris) Gothan; D. crassum (Menéndez) Petriella; Zuberia zuberi (Szajnocha) Frengüelli; Z. sp. cf. Z. barrealensis Frengüelli?, Peltaspermales ?Scytophyllum sp. cf. S. neuburgianum Dobruskina; Pachydermophyllum sp.; Peltaspermum sp.?, putative Gnetales ?Gontriglossa sp.?, Osmundales ?Cladophlebis mendozaensis (Geinitz) Frengüelli?, Equisetales, and two taxa of uncertain affinities. Although low in diversity, this assemblage is comparable to coeval assemblages in other basins, particularly the floras from the Agua de los Pajaritos, Barreal and Ischichuca formations, reinforcing the Middle Triassic age assigned by the vertebrate, microfloristic and radiometric dating. Key words. Fossil flora. Cerro de Las Cabras Formation. Cuyana Basin. Middle Triassic. Argentina","palabras_clave":"FOSSIL FLORA|CERRO DE LAS CABRAS FORMATION|CUYANA BASIN|MIDDLE TRIASSIC|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4670,"idioma_id":4,"titulo":"Population dynamics of an invasive tree, Sorbus aucuparia, in the understory of a Patagonian forest","resumen":"Exotic fleshy fruited plants are often dispersed by generalist frugivores into undisturbed or weakly disturbed forests. The establishment and spread of these plants in the understory may pass unnoticed until their abundance and size turns them a nuisance. Sorbus aucuparia is a fleshy fruited tree recently established in temperate forests of northwestern Patagonia. Our aim was to determine the invasion dynamics of S. aucuparia in a Nothofagus pumilio forest. We analyzed age structure, minimum age of reproduction, the time from colonization to effective recruitment (lag time), and demographic parameters to pinpoint critical stages for management. Currently, the population of S. aucuparia is increasing. Large numbers of suppressed, potentially reproductive individuals were found. Lag time was relatively short (25 years) in comparison to other invasive woody plants. The minimum reproductive age was 10 years, and all reproductive individuals were located in high light conditions. The number of trees at the reproductive stage made the most significant contribution to population growth; a 5 % reduction in this number would be enough for the population growth rate to be negatively affected. Thus, the reproductive stage is key to the control of S. aucuparia. Forest disturbance leading to increase light availability may result in higher rates of growth and reproduction among currently suppressed individuals, triggering further expansion events and increasing the number of invasion foci. Management strategies for tree species which present an inconspicuous bank of individuals in the understory, like S. aucuparia, should focus on early detection and eradication of reproductive individuals while avoiding the increase in light availability in the affected environment.","palabras_clave":"EXOTIC TREE|FLESHY FRUIT|LAG TIME|MATRIX DEMOGRAPHIC ANALISYS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4671,"idioma_id":5,"titulo":"¿Los hermanos sean unidos? Competitividad en las primarias municipales en la Provincia de Buenos Aires","resumen":"La discusión en torno al efecto de las elecciones primarias sobre los partidos divide a la literatura entre los que sostienen que sin importar el resultado interno, los votos del candidato perdedor se trasladan masivamente a votar luego por el ganador de la contienda, y quienes destacan que si la disputa al interior del partido\/coalición se traslada a una interna competitiva, el candidato que pierde no juega para su equipo con el consecuente impacto de que el partido encuentra disminuidas las chances de ganar la elección general.Por su propio diseño, la política es territorial en Argentina, y esta afirmación es especialmente relevante en los municipios de la provincia de Buenos Aires por ser el distrito más extenso, poblado y rico del país. El objetivo del presente trabajo es trasladar la discusión teórica acerca del efecto de la primaria en la elección general, a las elecciones de intendentes de la provincia de Buenos Aires. Se sostiene que las divisiones internas de los partidos\/coaliciones se expresan en la primaria de manera negativa, lo que impacta directamente en la posibilidad de ganar la elección municipal. A través de un modelo estadístico, el presente trabajo encuentra que cuanto más competitiva resulta la elección primaria dentro del partido\/coalición, menores son sus chances de ganar la intendencia.","palabras_clave":"COALICIONES|COMPETITIVIDAD|PRIMARIAS|SUBNACIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4672,"idioma_id":4,"titulo":"Biological control of Alphitobius diaperinus with Steinernema rarum CUL and Heterorhabditis bacteriophora SMC and feasibility of application in rice hull","resumen":"Chemical insecticides often are not efficient in reducing Alphitobius diaperinus populations in poultry farms and have induced insect resistance. Entomopathogenic nematodes (EPNs) appear as an alternative to pest biocontrol agents; however, their efficiency on rice hull litter is still unknown. The aim of this study was to evaluate the potential of Steinernema rarum (CUL isolate), Heterorhabditis bacteriophora (SMCisolate) and their symbiotic bacteria as biocontrol agents of A. diaperinus larvae and adults in rice hull litter. Mortality of insect adults and fifth- and seventh-instar larvae was determined in Petri dishes containing filter paper or rice hull. The effect of Xenorhabdus szentirmaii and Photorhabdus luminescens on L7 and adults in rice hull substrate was also evaluated. Moreover, the effectiveness of the EPNs was assessed in bags in an environment simulating the prevailing conditions in a broiler chicken farm. Under laboratory conditions, insect mortality was significantly affected by EPN species, substrate, insect stage and time after application. Mortality of L5 and L7 caused by EPNs in rice hulls reached a peak of 64 and 57.3 %, respectively, whereas symbiotic bacteria applied on rice hull showed low mortality rate at both insect stages. In the simulated poultry farm conditions, S. rarum and H. bacteriophora caused up to 40.8 and 50.4 % mortality of A. diaperinus larvae, respectively, but with adult mortality being low for both species. We conclude that infective juveniles from S. rarum CUL and H. bacteriophora SMC isolates can be used as biological control agents of A. diaperinus larvae in chicken litter containing rice hulls.","palabras_clave":"Entomopathogenic nematode|Alphitobius diaperinus|poultry production|biological control"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4673,"idioma_id":4,"titulo":"Morphological analysis of the females reproductive system of Stenorhynchus seticornis (Brachyura: Inachoididae) and comparisons with other Majoidea","resumen":"We examined the female reproductive system of the yellowline arrow crab Stenorhynchus seticornis by means of histological and histochemical techniques. Mature specimens obtained in the field were kept in the laboratory for mating experiments. After 24 h, newly mated females were dissected, and their reproductive trait routinely processed for embedding in historesin. The specimens examined each possessed a pair of kidney-shaped seminal receptacles (SR), and these we classified as ventral type, based on the location of the oviduct opening. The mesodermal dorsal region (DR) of SR consisted of a stratified epithelium with scaly cells, while the ectodermal ventral region (VR) was composed of a simple epithelium covered by a cuticle. The oviduct opened at the transition region (TR) between DR and VR and had no velum. The simple epithelium of TR had more folds on the face of the oviduct opening. The vagina exhibited the same features as the TR epithelium and was contiguous to VR, anchored by muscles. In the lumen, from one to three strata of sperm packets were observed, the dorsal one containing free sperm, and the most ventral stratum, spermatophores. An acidophilic glycoprotein layer enclosed the strata. Spermatophores in the ventral stratum were enclosed in a voluminous secretion, composed by acid polysaccharides most likely from the last male mated. The ventral-type receptacle, stratified sperm packets, and polyandry, usually observed in females of Majoidea, suggest the occurrence of sperm competition in S. seticornis, favoring the sperm of the last male mated, as its sperm mass is located near the opening of the female oviduct.","palabras_clave":"SEMINAL RECEPTACLES|MAJOIDEA|SPERM COMPETITION|FUNCTIONAL MORPHOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4675,"idioma_id":4,"titulo":"Temporal changes in the expression of the translocator protein TSPO and the steroidogenic enzyme 5\u0002-reductase in the dorsal spinal cord of animals with neuropathic pain: Effects of progesterone administration","resumen":"Neuropathic pain is a frequent complication of spinal cord injury (SCI), still refractory to conventionaltreatment. The presence and biological activity of steroidogenic regulatory proteins and enzymes in thespinal cord suggests that neurosteroids locally generated could modulate pain messages. In this studywe explored temporal changes in the spinal expression of the 18 kDa translocator protein TSPO, thesteroidogenic acute regulatory protein (StAr) and the steroidogenic enzyme 5-reductase (5-RI\/II) inan experimental model of central chronic pain. Male Sprague-Dawley rats were subjected to a SCI andsacrificed at different time points (1, 14 or 28 days). The development of mechanical and cold allodyniawas assessed. Injured animals showed an early increase in the mRNA levels of TSPO and 5-RII, whereasin the chronic phase a significant decrease in the expression of 5-RI and 5-RII was observed, coincidingwith the presence of allodynic behaviors. Furthermore, since we have shown that progesterone (PG)administration may offer a promising perspective in pain modulation, we also evaluated the expressionof steroidogenic proteins and enzymes in injured animals receiving daily injections of the steroid.PG-treated did not develop allodynia and showed a marked increase in the mRNA levels of TSPO, StAR,5-RI and 5-RII 28 days after injury. Our results suggest that in the acute phase after SCI, the increased","palabras_clave":"SPINAL CORD INJURY|STEROIDOGENESIS|PROGESTERONE|NEUROPATHIC PAIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4676,"idioma_id":4,"titulo":"Hsp90-binding immunophilin FKBP51 forms complexes with hTERT enhancing telomerase activity","resumen":"1 Both authors have contributed equally to this work. (Nadia R. Zgajnar AND Mariana I. Lagadari)FK506-binding proteins are members of the immunophilin family of proteins. Those immunophilins associated to the 90-kDa-heat-shock protein, Hsp90, have been proposed as potential modulators of signalling cascade factors chaperoned by Hsp90. FKBP51 and FKBP52 are the best characterized Hsp90-bound immunophilins first described associated to steroid-receptors. The reverse transcriptase subunit of telomerase, hTERT, is also an Hsp90 client-protein and is highly expressed in cancer cells, where it is required to compensate the loss of telomeric DNA after each successive cell division. Because FKBP51 is also a highly expressed protein in cancer tissues, we analyzed its potential association with hTERT·Hsp90 complexes and its possible biological role. In this study it is demonstrated that both immunophilins, FKBP51 and FKBP52, co-immunoprecipitate with hTERT. The Hsp90 inhibitor radicicol disrupts the heterocomplex and favors the partial cytoplasmic relocalization of hTERT in similar manner as the overexpression of the TPR-domain peptide of the immunophilin. While confocal microscopy images show that FKBP51 is primarily localized in mitochondria and hTERT is totally nuclear, upon the onset of oxidative stress, FKBP51 (but not FKBP52) becomes mostly nuclear colocalizing with hTERT, and longer exposure times to peroxide favors hTERT export to mitochondria. Importantly, telomerase activity of hTERT is significantly enhanced by FKBP51. These observations support the emerging role assigned to FKBP51 as antiapoptotic factor in cancer development and progression, and describe for the first time the potential role of this immunophilin favoring the clonal expansion by enhancing telomerase activity.","palabras_clave":"FKBP51|hTERT|hsp90|IMMUNOPHILIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4677,"idioma_id":4,"titulo":"Plasmin and coagulant activities in a minicurd model system. Study of technological parameters","resumen":"The effect of scalding temperature of the curd, the inclusion of a washing step, and the pH at whey drainage on plasmin and coagulant activities were assessed in a minicurd model of young hard cooked cheese. The variables were tested as follows: draining pH was assayed at 3 levels (4.6, 5.6, and 6.4), curd scalding temperature was tested at 50 and 56°C, and washing of the curd was examined at 2 levels (no washing step, and the replacement of the whey by water). Increase in pH at whey drainage and washing of the curd had a positive effect on plasmin activity, which was also evidenced by compatible changes in soluble peptide profiles. No effect of increased cooking temperature was found on plasmin activity. Plasminogen activation was not verified in any treatment. As for coagulant, lower pH values at whey drainage and a decrease in curd cooking temperature increased its activity; washing of the curd showed no influence on coagulant residual activity. These results were consistent with proteolysis described by peptide profiles, electrophoresis, and soluble nitrogen fractions.","palabras_clave":"PLASMIN |COAGULANT|MINICURD MODEL|HARD CHEESE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4678,"idioma_id":4,"titulo":"Algorithmic Pseudorandomness in Quantum Setups","resumen":"Many experimental setups in quantum physics use pseudorandomness in places where the theory requires randomness. In this work we show that the use of pseudorandomness instead of proper randomness in quantum setups has potentially observable consequences. First, we present a new loophole for Bell-like experiments: if some of the parties choose their measurements pseudorandomly, then the computational resources of the local model have to be limited in order to have a proper observation of non-locality. Second, we show that no amount of pseudorandomness is enough to produce a mixed state by computably choosing pure states from some basis.","palabras_clave":"pseudorandom mixtures|Bell loophole|computability|randomness"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4679,"idioma_id":4,"titulo":"Podocyturia is significantly elevated in untreated vs treated Fabry adult patients","resumen":"BACKGROUND:Proteinuria suggests kidney involvement in Fabry disease. We assessed podocyturia, an early biomarker, in controls and patients with and without enzyme therapy, correlating podocyturia with proteinuria and renal function.METHODS:Cross-sectional study (n = 67): controls (Group 1, n = 30) vs. Fabry disease (Group 2, n = 37) subdivided into untreated (2A, n = 19) and treated (2B, n = 18). Variables evaluated: age, gender, creatinine, CKD-EPI, proteinuria, podocyte count\/10 20× microscopy power fields, podocytes\/100 ml urine, podocytes\/g creatininuria (results expressed as median and range).RESULTS:Group 1 vs. 2 did not differ concerning age, gender and CKD-EPI, but differed regarding proteinuria and podocyturia. Group 2A vs. 2B: age: 29 (18-74) vs. 43 (18-65) years (p = ns); gender: males n = 3 (16 %) vs. n = 9 (50 %). Proteinuria was significantly higher in Fabry treated patients, while CKD-EPI and podocyturia were significantly elevated in untreated individuals. Significant correlations: group 2A: age-proteinuria, &#961; = 0.62 (p = 0.0044); age-CKD-EPI, &#961; = -0.84 (p < 0.0001); podocyturia-podocytes\/100 ml urine, &#961; = 0.99 (p = 0.0001); podocyturia-podocytes\/g creatininuria &#961; = 0.86 (p = 0.0003), podocytes\/100 ml urine-podocytes\/g urinary creatinine, &#961; = 0.84 (p = 0.0004); proteinuria-CKD-EPI, &#961; = -0.68 (p = 0.0013). Group 2B: podocyturia-podocytes\/100 ml urine, &#961; = 0.88 (p < 0.0001); podocyturia-podocytes\/g creatininuria, &#961; = 0.84 (p < 0.0001); podocytes\/100 ml urine-podocytes\/g creatininuria, &#961; = 0.94 (p < 0.0001); CKD-EPI-proteinuria, &#961; = -0.66 (p = 0.0028).CONCLUSIONS:Patients with Fabry disease display heavy podocyturia; those untreated present significantly higher podocyturia, lower proteinuria and better renal function than those who are treated, suggesting that therapy may be started at advanced stages. Podocyturia may antedate proteinuria, and enzyme therapy may protect against podocyte loss.","palabras_clave":"PODOCITURIA|FABRY|GL3|CREATININA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4680,"idioma_id":4,"titulo":"PhoB activation in non-limiting phosphate condition by the maintenance of high polyphosphate levels in stationary phase inhibits biofilm formation in Escherichia coli","resumen":"Polyphosphate (polyP) degradation in Escherichia coli stationary phase triggers biofilm formation via the LuxS quorum sensing system. In media containing excess of phosphate (Pi), high polyP levels are maintained in the stationary phase with the consequent inhibition of biofilm formation. The transcriptional-response regulator PhoB, which is activated under Pi limitation, is involved in the inhibition of biofilm formation in several bacterial species. In the current study, we report, for the first time, we believe that E. coli PhoB can be activated in non-limiting Pi conditions, leading to inhibition of biofilm formation. In fact, PhoB was activated when high polyP levels were maintained in the stationary phase, whereas it remained inactive when the polymer was degraded or absent. PhoB activation was mediated by acetyl phosphate with the consequent repression of biofilm formation owing to the downregulation of c-di-GMP synthesis and the inhibition of autoinducer-2 production. These results allowed us to propose a model showing that PhoB is a component in the signal cascade regulating biofilm formation triggered by fluctuations of polyP levels in E. coli cells during stationary phase.","palabras_clave":"POLYPHOSPAHTE|PHOB|BIOFILM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4681,"idioma_id":4,"titulo":"Effects of nitric oxide system and osmotic stress on Aquaporin-1 in the postnatal heart.","resumen":"Aquaporin-1 (AQP1) is expressed in the heart and its relationship with NO system has not been fully explored. The aims of this work were to study the effects of NO system inhibition on AQP1 abundance and localization and evaluate AQP1 S-nitrosylation in a model of water restriction during postnatal growth. Rats aged 25 and 50days (n=15) were divided in: R: water restriction; C: water ad libitum; RL: L-NAME (4mg\/kgday)+water restriction; CL: L-NAME+water ad libitum. AQP1 protein levels, immunohistochemistry and S-nitrosylation (colocalization of AQP1 and S-nitrosylated cysteines by confocal microscopy) were determined in cardiac tissue. We also evaluated the effects of NO donor sodium nitroprusside (SNP) on osmotic water permeability of cardiac membrane vesicles by stopped-flow spectrometry. AQP1 was present in cardiac vascular endothelium and endocardium in C and CL animals of both ages. Cardiac AQP1 levels were increased in R50 and RL50 and appeared in cardiomyocyte plasma membrane. No changes in AQP1 abundance or localization were observed in R25, but RL25 group showed AQP1 presence on cardiomyocyte sarcolemma. AQP1 S-nitrosylation was increased in R25 group, without changes in the 50-day-old group. Cardiac membrane vesicles expressing AQP1 presented a high water permeability coefficient and pretreatment with SNP decreased water transport. Age-related influence of NO system on AQP1 abundance and localization in the heart may affect cardiac water homeostasis during hypovolemic state. Increased AQP1 S-nitrosylation in the youngest group may decrease osmotic water permeability of cardiac membranes, having a negative impact on cardiac water balance.","palabras_clave":"NITRIC OXIDE|OSMOTIC STRESS|AQP1|HEART"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4682,"idioma_id":4,"titulo":"Atmospheric Mercury Concentrations observed at ground-based monitoring sites globally distributed in the framework of the GMOS network","resumen":"Long-term monitoring of data of ambient mercury (Hg) on a global scale to assess its emission, transport, atmospheric chemistry, and deposition processes is vital to understanding the impact of Hg pollution on the environment. The Global Mercury Observation System (GMOS) project was funded by the European Commission (http:\/\/www.gmos.eu) and started in November 2010 with the overall goal to develop a coordinated global observing system to monitor Hg on a global scale, including a large network of ground-based monitoring stations, ad hoc periodic oceanographic cruises and measurement flights in the lower and upper troposphere as well as in the lower stratosphere. To date, more than 40 ground-based monitoring sites constitute the global network covering many regions where little to no observational data were available before GMOS. This work presents atmospheric Hg concentrations recorded worldwide in the framework of the GMOS project (2010-2015), analyzing Hg measurement results in terms of temporal trends, seasonality and comparability within the network. Major findings highlighted in this paper include a clear gradient of Hg concentrations between the Northern and Southern hemispheres, confirming that the gradient observed is mostly driven by local and regional sources, which can be anthropogenic, natural or a combination of both.","palabras_clave":"Atmospheric Mercury|GMOS|Network"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4683,"idioma_id":4,"titulo":"Secretor status Fut 2 gene and ABH antigens in urogenital tumors","resumen":".","palabras_clave":"SECRETOR  STATUS|FUT 2 GENE|ABH ANTIGENS|TUMORS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4684,"idioma_id":5,"titulo":"Estudio comparativo de abrigos rocosos en la costa norte de Santa Cruz (Patagonia)","resumen":"En este trabajo se presenta un estudio comparativo de los abrigos rocosos identificados en la Costa Norte de Santa Cruz (Patagonia Argentina) a partir de un programa sistemático de relevamiento de este tipo de sitios, tanto en la costa, como en la franja intermedia. Mediante una ficha descriptiva se registraron variables contextuales, naturales y arqueológicas para poder analizar y comparar distintos aspectos de interés. De esta forma se relevaron 38 abrigos rocosos, de los cuales 26 brindaron evidencias arqueológicas, entre ellos 5 con manifestaciones rupestres. Los abrigos rocosos se ubican en un sector acotado del área de estudio, comprendido entre la cuenca del río y la ría Deseado y la costa ubicada al sur, asociados a la presencia de afloramientos ignimbríticos. Las evidencias obtenidas hasta el momento reflejan bajas densidades arqueológicas en la mayoría de los sitios. En este sentido, en general se identificaron escasos restos faunísticos y sucesiones de fogones pequeños y discretos, lo que sugiere que los eventos de ocupación de los mismos habrían sido relativamente poco intensivos.","palabras_clave":"Abrigos rocosos|Río Deseado|Patagonia|Cazadores recolectores"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4685,"idioma_id":4,"titulo":"Interspecific interactions between two Tuta absoluta (Lepidoptera: Gelechiidae) larval parasitoids with contrasting life histories","resumen":"partiallyoverlapping host niches, theidiobiont endoparasitoid Dineulophus phthorimaeae De Santis and the koinobiont endoparasitoid Pseudapanteles dignus (Muesebeck), werestudied. T absoluta is an important pest of tomato cropsworldwide, and its management could be improved by understanding thecompetitive interactions and potential coexistence between these twoparasitoids. First, a 15-min fixed time test was conducted in the laboratory toevaluate host search ability of adult D.phthorimaeae wasps on T. absolutalarvae previously exposed to P. dignus.Later, D. phthorimaeae hostdiscrimination against parasitised and non-parasitised hosts, at differentadult female ages, was experimentally examined. D. phthorimaeae wasps spent significantly more timein general searching in the presence of its competitor than in its absence;however, parasitism was only effective by P.dignus. Older D. phthorimaeaewasps discriminated significantly less than young wasps between T. absolutalarvae parasitised or unparasitised by P. dignus,. In the laboratory an interactiontook place by non-concurrent host-feeding. Intra-guild predation of P. dignus larvae by D.phthorimaeae female feeding behaviour might have a minor effect in thissystem. Resultsare discussed in the context of literature supporting diverse evidenceof coexistence in other parasitoid-host systems, with implications for T. absoluta biological control. &nbsp;","palabras_clave":"Dineulophus phthorimaeae|Pseudapanteles dignus|South American tomato moth|parasitoid oviposition behaviour"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4686,"idioma_id":4,"titulo":"Controlled preparation and characterization of Pt-Rh\/Al2O3 bimetallic catalysts for reactions in reducing conditions","resumen":"Pt-Rh bimetallic catalysts on alumina support were prepared according to three different techniques,one leading to random deposition of the two metals at the support?s surface, the two others, namely theorganometallic grafting and the colloidal route, favoring the formation of bimetallic particles with strongPt-Rh interactions. Catalysts were characterized by ICP, H2chemisorption, TPR, FTIR of chemisorbedCO and NO-CO. Then they were evaluated in cyclohexane dehydrogenation and methylcyclopentanehydrogenolysis reactions. Strong interaction between Pt and Rh was demonstrated for catalysts preparedby controlled preparation method, which leads to a strong synergetic effect for methylcyclopentane ring-opening, in terms of activity and selectivity, whereas these interactions do not play a role in cyclohexanedehydrogenation.","palabras_clave":"Bimetallic supported catalysts|Pt-Rh|Cyclohexane dehydrogenation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4687,"idioma_id":4,"titulo":"Antinociceptive, anti-inflammatory effects and safety of Ziziphus mistol fruits","resumen":"Ziziphus mistol Griseb. (Rhamnaceae), popularly known as ?mistol,? is widely distributed throughout Perú, Bolivia, Paraguay and Argentina. Its fruit is consumed in different forms in several argentinean communities. The present study was carried out to investigate the medicinal properties and safety of Ziziphus mistol (mistol) fruits ethanol and aqueous extracts and arrope. Antinociceptive activity was assessed using the formalin, acetic acid-induced writhing and tail-&#64258;ick tests in rats. Anti-in&#64258;ammatory effects were determinated through carrageenan induced edema test and cotton pellet-induced granuloma formation, in rats. The safety was evaluated with test of acute toxicity (48 hs) and sub-chronic toxicity (91 days).All extracts (1,000 mg \/ kg body weight) showed significant inhibition (P <0.05) in the pain and inflammation experimentally induced. In the acute toxicity study, a single dose of 4000 and 8000 mg\/kg b.w., produced no mortality and no clinical signs of disease were observed after 48 h. In the sub-chronic toxicity study the extracts no caused significant visible signs of toxicity, nor mortality for 91 consecutive days of treatment. No significant differences were found in relative organ weights and hematological and biochemical parameters between the treated groups and the control groups. Aqueous extract and arrope of Z. mistol fruits could be good source of antinociceptive and anti-inflamatory agents because of its good activity and safety.","palabras_clave":"ANTINOCICEPTIVE|ANTIINFLAMMATORY|ZIZIPHUS MISTOL|SAFETY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4688,"idioma_id":4,"titulo":"Setting the Agenda: Different strategies of a Mass Media in a model of cultural dissemination","resumen":"Day by day, people exchange opinions about a given new with relatives,friends, and coworkers. In most cases, they get informed about a given issue byreading newspapers, listening to the radio, or watching TV, i.e., through aMass Media (MM). However, the importance of a given new can be stimulated bythe Media by assigning newspaper´s pages or time in TV programs. In this sense,we say that the Media has the power to 'set the agenda', i.e., it decides whichnew is important and which is not. On the other hand, the Media can knowpeople´s concerns through, for instance, websites or blogs where they expresstheir opinions, and then it can use this information in order to be moreappealing to an increasing number of people. In this work, we study differentscenarios in an agent-based model of cultural dissemination, in which a givenMass Media has a specific purpose: To set a particular topic of discussion andimpose its point of view to as many social agents as it can. We model this bymaking the Media has a fixed feature, representing its point of view in thetopic of discussion, while it tries to attract new consumers, by takingadvantage of feedback mechanisms, represented by adaptive features. We exploredifferent strategies that the Media can adopt in order to increase the affinitywith potential consumers and then the probability to be successful in imposingthis particular topic.","palabras_clave":"Agent Based Modeling|Axelrod Model|Mass Media Influence|Emergent Collective Behavior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4689,"idioma_id":4,"titulo":"Halogen bonding. The role of the polarizability of the electron-pair donor","resumen":"The nature of F?Br  X?R interactions (with X = F, Cl, Br, I and R = ?H, ?F) has been investigated through theoretical calculation of molecular potential electrostatic (MEP), molecular polarizability, atoms in molecules (AIM) analysis and energetic decomposition analysis (EDA). A detailed analysis of the MEPs reveals that considering only the static electrostatic interactions is not sufficient to explain the nature of these interactions. The molecular polarizabilities of X?R molecules suggest that the deformation capacity of the electronic cloud of the lone pairs of the X atom plays an important role in the stability of these complexes. The topological analysis of the L(r) function and the detailed analysis of the atomic quadrupole moments reveal that the Br...X interactions are electrostatic in nature. The electron acceptor Br atom causes a polarization of the electronic cloud (electronic induction) on the valence shell of the X atom. Finally, the electrostatic forces and charge transfer play an important role not only in the stabilization of the complex, but also in the determination of the molecular geometry of equilibrium. The dispersive and polarization forces do not influence the equilibrium molecular geometry.","palabras_clave":"Halogen Bond|QTAIM|Laplacian|MEP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4690,"idioma_id":4,"titulo":"Do neophobia and dietary wariness explain ecological flexibility? An analysis with two seed-eating birds of contrasting habits","resumen":"The neophobia threshold hypothesis (NTH) suggests that the acquisition and maintenance of a high behavioral and ecological flexibility in the evolutionary and adaptive history of a species is the consequence of lower levels of neophobia towards new micro-habitats and of dietary wariness of novel foods. To test this idea we assessed the degree of neophobia and dietary wariness in two seed-eating bird species with contrasting degrees of ecological flexibility that inhabit the central Monte desert (Argentina): a grass-seed specialist, the many-colored chaco-finch, and a generalist feeder, the rufous-collared sparrow. We expected that both species would exhibit neophobia and wariness whenfaced with new foraging opportunities but that the rufous-collared sparrow would be less neophobic and less wary than the specialized many-colored chaco-finch. Experimental indicators of neophobia and dietary wariness included willingness to eat near novel objects and willingness to eat novel seeds, respectively. Both species showed similar levels of reluctance to novelty, although the sparrow could be slightly more reluctant than the finch. Contrary to our predictions, the sparrow was neither less hesitant nor faster or greedier than the finch. This experimental evidence does not support a negative relationship between neophobia \/ wariness and ecological flexibility in these two seed eatingbirds and it coincides with the growing evidence that challenges the NTH. Some of our results provide support for the dangerous niche hypothesis, especially as the rufous-collared sparrow, that feeds on more diverse and potentially dangerous food, showed higher levels of neophobia in some cases. Although the idea of neophobia and wariness being plausible causes of ecological specialization sounds attractive, the current situation calls for further research so that the causes of ecological flexibility in granivorous birds can be better understood.","palabras_clave":"Neophobia threshold hypothesis|ecological flexibility|dietary wariness|seed-eating birds"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4691,"idioma_id":5,"titulo":"Implementación de técnica de cultivo para la investigación de Legionella pneumophila en depósitos domiciliarios de agua potable en Resistencia, Chaco","resumen":"Legionella pneumophila es una bacteria ambiental capaz de sobrevivir en un amplio intervalo de condiciones físico-químicas y de colonizar los sistemas de distribución y almacenamiento del agua potable. Es el principal patógeno trasmitido por el agua que produce el 90% de los casos de legionelosis. El objetivo del trabajo fue realizar la puesta a punto de la técnica por cultivopara la vigilancia de L. pneumophila en depósitos domiciliarios de agua potable acorde con la normativa internacional. En las muestras de agua analizadas no se obtuvo desarrollo de L.pneumophila; la cepa utilizada como control positivo, permitió constatar la aptitud de los medios utilizados para la detección de este patógeno en las muestras de agua. La vigilancia de este microorganismo en el agua de consumo humano representa el primer paso en pos de abordar el control de su diseminación hacia huéspedes susceptibles.","palabras_clave":"Legionella|Agua potable|Depósitos domiciliarios"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4692,"idioma_id":5,"titulo":"Los Ursidae fósiles de América del Sur","resumen":"Los osos fósiles de América del sur están representados por la subfamilia Tremartinae, y se registran desde el Ensenadense (Pleistoceno temprano ?Pleistoceno medio) al Lujanense (Pleistoceno Superior- Holoceno Inferior); arribaron a América del sur durante el Gran Intercambio Biótico Americano, donde experimentaron una importante radiación adaptativa. A lo largo de la historia evolutiva de los tremarctinos en América del Sur se aprecia una notable reducción del tamaño corporal asociado a una tendencia a la hipocarnivoría, como posible una respuesta a la complejización del gremio de los carnívoros durante Pleistoceno medio y tardío. Los osos fósiles sudamericanos usaban cuevas excavadas por la megafauna endémica como madrigueras o para buscar alimentos y posiblemente hibernaban. No existen estudios filogenéticos que abarquen toda la diversidad del grupo, por esta razón se están llevando a cabo estudios en la región auditiva a fin de obtener caracteres que permitan un análisis filogenético.","palabras_clave":"Ursidae|Tremarctinae|Evolución |Region auditiva"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4693,"idioma_id":4,"titulo":"Magnetic properties and environment sites in Fe doped SnO2 nanoparticles","resumen":"Sn1&#8722;xFexO2 nanoparticles (x = 0, 0.05, 0.10 and 0.15) were synthesized by co-precipitation. X-ray diffraction and electron diffraction images showed that only rutile type phase was present in the samples. Electron microscopy was used as a tool for revealing morphology distribution size and structure characteristics of the nanopartiles; while Energy Dispersive X-Ray Spectroscopy analysis served to confirm the selected stoichiometry. 57Fe and 119Sn Mössbauer spectroscopy at Room Temperature showed a lattice disorder induced by the incorporation of Fe ions in the structure. No magnetic hyperfine ordering was detected for 57Fe probes and the asymmetry in their quadrupolar splittings is explained as a texture effect at the nanoparticles shells. Quadrupolar splitting at 119Sn probe reached a maximum for x=0.1, being this fact attributable to a highest distorted environment. Magnetic hysteresis loops were measured at different temperatures showing in Fe doped samples a combination of paramagnetism and weak ferromagnetism","palabras_clave":"MAGNETIC NANOPARTICLES|DILUTED MAGNETIC SEMICONDUCTORS|CHEMICAL METHOD|FE DOPED SNO2|MOSSBAUER SPECTROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4694,"idioma_id":5,"titulo":"Síntesis y caracterización de nanopartículas de ferrita de cobalto obtenidas en una plantilla de alúmina porosa bajo condiciones de confinamiento","resumen":"En este trabajo se reporta la síntesis y caracterización de nanopartículas magnéticas de CoFe2O4 que crecen en condiciones de confinamiento. Estas condiciones se logran al infiltrar una solución acuosa con los iones precursores de interés en una plantilla de alúmina anódica nanoporosa. Se estudian la morfología, composición química, estructura cristalina y las propiedades magnéticas del material sintetizado. Los resultados obtenidos revelan la formación de nanopartículas magnéticas aglomeradas con forma elipsoidal dentro y en la superficie del sustrato poroso. Debido al efecto de confinamiento, las fases sintetizadas dentro de los poros son diferentes de aquellas obtenidas en condiciones ambiente. Una comparación cuantitativa entre las anisotropías magnetocristalina y la de forma permite entender la invariancia de las curvas de histéresis respecto a la dirección como se aplica el campo magnético. El comportamiento superparamagnético de los aglomerados de nanopartículas se analiza por medio de curvas M vs. T a diferentes campos aplicados.","palabras_clave":"alúmina anódica|nanoreactor|anisotropía|superparamagnetismo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4695,"idioma_id":5,"titulo":"Fabricación de alúmina anódica porosa de bajo costo: Un estudio comparativo de la morfología producida por uno y dos pasos de anodizado","resumen":"SeSSe describe un método simple y barato para la fabricación de moldes de alúmina nanoporosa, con potenciales aplicaciones para la fabricación de nanohilos. Se utiliza como materia prima un aluminio de grado comercial. Los  moldes  nanoporosos  fabricados  tienen  un  diámetro  promedio  de  poros  de  (20  ±  4)  nm.  El  proceso  de fabricación consta de dos etapas de anodizado. Se detallan ambas etapas de anodizado y se caracteriza el material que se obtiene como resultado de cada una de ellas. Los moldes nanoporosos obtenidos con dos pasos de anodizado tienen la distribución más estrecha de tamaño de poros, mejor circularidad y orden espacial. En dichos  moldes  se  crecen  nanohilos  a  base  de  ferrita  de  cobalto  (CoFe2O4).  Para  hacerlo,  se  usa  un  método simple y un equipamiento sencillo. Mediante microscopía electrónica se hace una caracterización morfológica de las plantillas y de los nanohilos obtenidos","palabras_clave":"Alúmina nanoporosa|sustratos PAA|moldes nanoporosos|nanohilos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4696,"idioma_id":4,"titulo":"Carbon and water vapor balance in a subtropical pine plantation","resumen":"Afforestation has been proposed as an effective tool for protecting primary and\/or secondary forests and for mitigating atmospheric CO2. However, the dynamics of primary productivity differs between plantations and natural forests. The objective of this work was to evaluate the potential for carbon storage of a commercial pine plantation by determining its carbon balance. Measurements started when trees were aged 6 and ended when they were older than 8 years. We measured CO2 and water vapor concentrations using the Eddy covariance method. Gross primary productivity in 2010 and 2011 was 4290 ± 473 g C m-2 and 4015 ± 485 g C m-2, respectively. Ecosystem respiration ranged between 7 and 20 g C m-2 d-1, reaching peaks in all Februaries. Of the 30 months monitored, the plantation acted as carbon source for 21 months and as carbon sink for 6 months, while values close to neutrality were ob- tained during 3 months. The positive balance representing CO2 loss by the sys- tem was most likely due to the cut branches left on the ground following prun- ing activities. The plantation was subjected to pruning in January and Septem- ber 2008 and to sanitary pruning in October 2010. In all cases, cut branches were not removed but remained on the ground. Residue management seems to have a very important impact on carbon balance.","palabras_clave":"afforestation|carbon source|ecosystem respiration |prunning|thinning"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4697,"idioma_id":4,"titulo":"Remote sensing and ground-based measurements of evapotranspiration in an extreme cold Patagonian desert","resumen":"Accurate estimates of seasonal evapotranspiration (ET) at different temporal and spatial scales are essential for understanding thebiological and environmental determinants of ecosystem water balance in arid regions and the patterns of water utilization by thevegetation. For this purpose, remote sensing ET estimates of a Patagonian desert in Southern Argentina were verified with fieldmeasurements of soil evaporation and plant transpiration using an open top chamber. Root distribution and seasonal variation insoil volumetric water content were also analysed. There was a high correlation between remote sensing and field measurementsof ecosystem water fluxes. A substantial amount of the annual ET occurred in spring and early summer (73.4 mm) using winterrain stored in the soil profile and resulting in water content depletion of the upper soil layers. A smaller amount of annual ET wasderived from few rainfall events occurring during the mid or late summer (41.4 mm). According to remote sensing, the 92.9% ofthe mean annual precipitation returns to the atmosphere by transpiration or evaporation from the bare soil and by canopyinterception. Only 7.1% infiltrates to soil layers deeper than 200 cm contributing to the water table recharge. Fourier time seriesanalysis, cross-correlation methods and multiple linear regression models were used to analyse 11 years of remote sensing data toassess determinants of water fluxes. A linear model predicts well the variables that drive complex ecosystem processes such asET. Leaf area index and air temperature were not linearly correlated to ET because of the multiple interaction among variablesresulting in time lags with ET variations and thus these two variables were not included in the linear model. Soil water content,the fraction of photosynthetic active radiation and precipitation explained 86% of the ET monthly variations. The highvolumetric water content and the small seasonal variations at 200-cm depth were probably the result of little water uptake fromdeeper soil horizons by roots with low hydraulic conductivity","palabras_clave":"MODIS|PRECIPITATION PULSES|ROOT DISTRIBUTION|WATER BALANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4698,"idioma_id":4,"titulo":"The significance of specimen displacement definition on the mechanical properties derived from Small Punch Test","resumen":"The different displacements definitions used for describing the Small Punch Tests (SPT) material response were thoroughly reviewed. For that purpose, an instrumented experimental setup allowing determining the specimens top or bottom displacement together with the machine cross head displacement was designed. The effects of the machine and load train compliances on the respective displacements values were carefully evaluated. The significance of the different displacements definitions was demonstrated by proposing two alternative procedures for assessing the specimen thickness evolution and by revising the definition of the load associated with the determination of the material yield stress from SPT. To facilitate the systematic study of the influence of the different parameters that might be affecting the test, a finite element (FEM) analysis tool was developed. The results obtained from FEM simulations show that even using a constitutive model that does not include damage initiation it is possible to adequately reproduce the SPT load vs. displacement response curve to near maximum load and also the diametral profiles of the deformed specimens. The FEM analysis indicate the arbitrary character of the different proposals for determination of the load for yield stress estimation with them corresponding to different degrees of plastic deformation of the specimen.","palabras_clave":"SMALL PUNCH TESTING|REDUCED SIZE SPECIMEN|MECHANICAL CHARACTERIZATION|FINITE ELEMENT METHOD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4699,"idioma_id":4,"titulo":"Use of 454-Pyrosequencing for the Characterization of Sweet Potato Virus C and Sweet Potato Feathery Mottle Virus Isolates from Argentina and Development of a Multiplex One-Step RT-PCR for Their Simultaneous Detection","resumen":"454-pyrosequencing was applied as a tool to identify etiological agentsinvolved in the symptoms observed in a sweet potato sample from Argentina.RNA was purified from symptomatic material and sequenced using aRoche 454 GS-FLX+. BLAST analysis of the viral reads identified the presenceof Sweet potato feathery mottle virus (SPFMV)-O and SPFMV-RC strainsand Sweet potato virus C (SPVC). For SPFMV-O and SPFMV?RC, 10 878and 10 812 nucleotides, respectively, were sequenced, whereas asequence of 10 793 nucleotides was obtained for SPVC. Pairwise comparisonof polyprotein nucleotide sequences of O and RC Argentinian isolates(Arg) showed 99% and 98.4% sequence identities with SPFMV-O andSPFMV-RC-M2-41, respectively, whereas SPVC-Arg showed 94.2%,92.9% and 94.6% sequence identities with SPVC-Il, SPVC-C1 and SPVCBungo,respectively. These results allowed us to develop a one-step multiplexreverse transcription-polymerase chain reaction assay (mRT-PCR) forthe simultaneous detection and differentiation of SPFMV-O-Arg andSPFMV-RC-Arg and SPVC-Arg. Three specific forward primers unique toeach virus and one reverse primer based on a region conserved in all threeviruses were designed and used in the assay. These primers were furtherevaluated using field samples collected from four provinces of Argentina.The results showed that SPVC was the most common virus in samplesanalysed. This study shows the usefulness of deep sequencing not only torapidly identify mixed infections between SPFMV-O and SPFMV-RC andSPVC, but also to develop a reliable mRT-PCR assay for detection of thesesweet potato pathogens, which cannot be discriminated by serologicaltechniques.","palabras_clave":"454-PYROSEQUENCING|MOLECULAR CHARACTERIZATION|MRT-PCR|POTYVIRUS|SWEET POTATO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4700,"idioma_id":4,"titulo":"Hydrolysis of phenyl trifluoroacetate in mixed surfactant systems","resumen":"T","palabras_clave":"mixed surfactant|hydrolysis|micelle structure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4701,"idioma_id":4,"titulo":"Structure characterization of non-crystalline complexes of copper salts with native cyclodextrins","resumen":"The characterization of non-crystalline complexes is particularly difficult when techniques like X-ray diffraction or NMR cannot be used. We propose a simple procedure to characterize the physicochemical properties of amorphous new coordination compounds between cyclodextrins (CD) and Cu2+ salts, by means of the integration of the information provided by several techniques including elemental analysis, flame atomic absorption, TGA, UV-Vis diffuse reflectance, colorimetry, FT-IR and EPR. On the basis of these procedures, we suggest geometrical and structural approximations resulting in an octahedral or distorted octahedral geometry with diverse positions for the metallic centre. According to the EPR spectrum, only one of the complexes may have rhombic symmetry. We also analyzed enthalpy?entropy compensation and the isokinetic effect. In addition, general trends in thermal stability, spectroscopic properties and inclusion in the cavity were analysed. This complete characterization methodology becomes essential for their future application as catalysts.","palabras_clave":"cyclodextrin|metal organic complexes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4702,"idioma_id":5,"titulo":"Primer reporte de Sweet potato leaf curl virus en las provincias de Córdoba y Santiago del Estero de Argentina","resumen":"La superficie con batata en Argentina experimentó notable reducción, causada por virosis que ocasionan daños en todas las regiones productoras, debido al intercambio indiscriminado de material de propagación y consiguiente ingreso de virus antes no citados, tales como los begomovirus de batata (sweepovirus), recientemente caracterizados en Bella Vista, Corrientes.En este trabajo, se analizaron secuencias parciales del genoma de dos aislamientos del Centro de Argentina, con las de uno previamente caracterizado en nuestro país (Bella Vista) y con las de otros begomovirus citados globalmente. Ambos aislamientos mostraron 97-99% de identidad de nucleótidos con Sweet potato leaf curl virus, de Bella Vista (JQ349087.1), por lo que según lo establecido por el International Committee on Virus Taxonomy (ICTV), ambos pertenecen a la misma raza. Sin embargo, es la primera vez que se caracteriza a este patogéno en la provincia de Córdoba y Santiago del Estero, Argentina; por lo que este trabajo es un aporte al esclarecimiento del complejo panorama de virosis en cultivo de batata en nuestro país.","palabras_clave":"IPOMOEA BATATAS|BEGOMOVIRUS|PCR|CLONADO|SECUENCIACIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4703,"idioma_id":4,"titulo":"Spin density wave instabilities in the NbS2 monolayer","resumen":"In the present work, we study the magnetic properties of the NbS 2 monolayer by first-principles calculations.The transition metal dichalcogenides (TMDCs) are a family of laminar materials presenting exciting propertiessuch as charge density waves (CDWs), superconductivity, and metal-insulating transitions. 2H -NbS 2 is aparticular case within the family, because it is the only one that is a superconductor without exhibiting aCDW order. Although no long-range magnetic order was experimentally observed in the TMDCs, we show herethat the single monolayer of NbS 2 is on the verge of a spin density wave (SDW) phase. Our calculations indicatethat a wavelike magnetic order is stabilized in the NbS 2 monolayer in the presence of magnetic defects or withinzigzag nanoribbons, due to the presence of unpaired electrons. We calculate the real part of the bare electronicsusceptibility and the corresponding nesting function of the clean NbS 2 monolayer, showing that there are strongelectronic instabilities at the same wave vector associated with the calculated SDWs, also corresponding with oneof the main nesting vectors of the Fermi surface. We conclude that the physical mechanism behind the spin-waveinstabilities are the nesting properties, accentuated by the quasi-2D character of this system, and the rather strongCoulomb interactions of the 4d band of the Nb atom. We also estimate the amplitude of the spin fluctuations andfind that they are rather large, as expected for a system on the verge of a quantum critical transition.","palabras_clave":"Spin density waves|transition metal dichalcogenides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4704,"idioma_id":4,"titulo":"Lattice specific heat for the RMIn 5 (R =Gd, La, Y; M=Co, Rh) compounds: Non-magnetic contribution subtraction","resumen":"We analyze theoretically a common experimental process used to obtain the magnetic contribution to the specific heat of a given magnetic material. In the procedure, the specific heat of a non-magnetic analog is measured and used to subtract the non-magnetic contributions, which are generally dominated by the lattice degrees of freedom in a wide range of temperatures.We calculate the lattice contribution to the specific heat for the magnetic compounds GdMIn5 (M=Co, Rh) and for the non-magnetic YMIn5 and LaMIn5 (M=Co, Rh), using density functional theory based methods. We find that the best non-magnetic analog for the subtraction depends on the magnetic material and on the range of temperatures. While the phonon specific heat contribution of YRhIn5 is an excellent approximation to the one of GdCoIn5 in the full temperature range, for GdRhIn5 we find a better agreement with LaCoIn5, in both cases, as a result of an optimum compensation effect between masses and volumes. We present measurements of the specific heat of the compounds GdMIn5 (M=Co, Rh) up to room temperature where it surpasses the value expected from the Dulong?Petit law. We obtain a good agreement between theory and experiment when we include anharmonic effects in the calculations.","palabras_clave":"115|COMPOUNDS|PHONONS|DFT|AB INITIO|MAGNETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4705,"idioma_id":4,"titulo":"Three dimensional flow of liquid transfer between a cavity and a  moving roll","resumen":"Gravure printing is one of the most promising technologies for high volume production of printed electronics and microscale films and devices.  The characteristics of the printed pattern, i.e. ink volume, resolution andpattern placement (registration), are directly related to the fluid mechanics of the liquid transfer process from a cell to a substrate wrapped around a rotating roll; the liquid transfer is mainly controlled by free surfaces and dynamic contact lines.  Most of the available analyses are restricted to axisymmetric flows, at which the relative motion between the cavity and the substrate is greatly simplified.  Recent results have shown that the use of the complete description of the relative motion in a roll-to-roll process is critical to obtain accurate results on the amount of liquid that is transferred to the substrate. In this work we present an extension of the model describing liquid transfer from a groove to a substrate in a R2R process in order to consider the liquid transfer from a small individual cell; to this end we solve a full 3D free surface flow with moving contact lines. The results show that the liquid transfer dynamics is governed by two different characteristic time scales, one is associated with the contact line motion and the other with liquid filament breakup.  Both are dependent on the capillary number.  The predictions show how the volume, registration and shape of the printed dot varies with operating conditions and liquid properties. These predictions could be helpful in designing high precision printing operations.","palabras_clave":"gravure printing|liquid transfer|contact line|printed  pattern|finite element method|three-dimensional free surface flow"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4706,"idioma_id":4,"titulo":"ICPMS analysis of proteins separated by Native-PAGE: Evaluation of metaloprotein profiles in human synovial fluid with acute and chronic arthritis","resumen":"The role of trace elements bound to proteins in the etiology and pathogenesis of rheumatoid arthritis (RA) remains unclear. In this sense, the identification and detection of metalloproteins has a strong and growing interest. Metalloprotein studies are currently carried out by polyacrylamide gel electrophoresis (PAGE) associated to inductively coupled plasma mass spectrometry (ICPMS), and despite that complete information can be obtained for metals such as Fe, Cu and Zn, difficulties due to poor sensitivity for other trace elements such as Sn, As, etc, are currently faced. In the present work, a simple and fast method for the determination of trace metals bound to synovial fluid (SF) proteins was optimized. Proteins from SF (long and short-term RA) were separated in ten fractions by native PAGE, then dissolved in nitric acid and peroxide hydrogen, and analyzed by ICPMS. Fifteen metals were determined in each separated protein fraction (band). Adequate calibration of proteins molecular weight allowed stablishing which protein type were bound to different metals.","palabras_clave":"HUMAN SYNOVIAL FLUID PROTEINS|TRACE ELEMENTS|POLIACRILAMIDE GEL ELECTROPHORESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4707,"idioma_id":4,"titulo":"Simultaneous chromate and sulfate removal by Streptomyces sp. MC1. Changes in intracellular protein profile induced by Cr(VI)","resumen":"The purpose of this study was to investigate the influence of increasing sulfate concentrations on chromium removal, to evaluate the effect of the presence of Cr(VI) on sulfate removal by Streptomyces sp. MC1 and to analyze the differential protein expression profile in the presence of this metal for the identification of proteins repressed or overexpressed. In the presence of Cr(VI)but in the absence of sulfate ions, bacterial growth was negligible, showing the Cr(VI) toxicity for this bacterium. However, the sulfate presence stimulated bacterium growth and Cr(VI) removal,regardless of its concentrations. Streptomyces sp. MC1 showed ability to remove chromium and sulfate simultaneously. Also, the sulfate presence favored the decrease of total chromium concentration from supernatants reaching a decrease of 50% at 48 h. In presence of chromium,seven proteins were down-expressed and showed homology to proteins involved in protein biosynthesis, energy production and free radicals detoxification while two proteins involved in oxidation-reduction processes identified as dihydrolipoamide dehydrogenase and S-adenosyl-Lmethioninesynthase were overexpressed.","palabras_clave":"ACTINOBACTERIA|CHROMIUM REMOVAL|SULFATE REMOVAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4710,"idioma_id":4,"titulo":"Differential hormonal and gene expression dynamics in two inbred sunflower lines with contrasting dormancy level","resumen":"Seed germination and dormancy are tightly regulated by hormone metabolism and signaling pathway.We investigated the endogenous content of abscisic acid (ABA), its catabolites, and gibberellins (GAs), as well as the expression level of certain ABA and GAs metabolic and signaling genes in embryo of dry andimbibed cypselas of inbred sunflower (Helianthus annuus L., Asteraceae) lines: B123 (dormant) and B91 (non-dormant). Under our experimental conditions, the expression of RGL2 gene might be related to the ABA peak in B123 line at 3 h of imbibition. Indeed, RGL2 transcripts are absent in dry and early embeddedcypselas of the non-dormant line B91. ABA increase was accompanied by a significant ABA-Glucosyl ester (ABA-GE) and phaseic acid (PA) (two ABA catabolites) decrease in B123 line (3 h) which indicates that ABA metabolism seems to be more active in this line, and that it would be involved in the imposition and maintenance of sunflower seed dormancy, as it has been reported for many species. Finally, an increase of bioactive GAs (GA1 and GA3) occurs at 12 h of imbibition in both lines after a decrease in ABA content.This study shows the first report about the RGL2 tissue-specific gene expression in sunflower inbred lines with contrasting dormancy level. Furthermore, our results provide evidence that ABA and GAs contentand differential expression of metabolism and signaling genes would be interacting in seed dormancy regulation through a mechanism of action related to embryo itself.","palabras_clave":"Dormancy|Gene expression|Germination|Helianthus annuus|Phytohormones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4711,"idioma_id":4,"titulo":"Antioxidant effect of bovine plasma proteins modified via Maillard reaction on n-3 fortified beef patties","resumen":"This work aimed to develop a healthier meat product, by improving nutritional properties related to lipid profile, using a Maillard reaction product (MRP) obtained from recycling blood from local slaughterhouses as antioxidant. Soybean oil was used as partial fat replacer and the lipid profile was modified as a decrease in the n6\/n3 ratio (11.59 to 9.50), and atherogenic and trombogenic index (from 0.42 to 0.27 and 0.94 to 0.56 respectively) was observed. Beef patties were added with two MRP levels (1% w\/w and 3% w\/w). Patties with no antioxidants were used as control and patties containing 0.01% w\/w of butylhydroxyanisole (BHA) were used as reference. Oxidative stability of refrigerated vacuum-packed patties was evaluated during 15 days of storage. Results showed 3% of MRP as the most appropriate concentration to prevent lipid oxidation with inhibition percentages of 75%, and 9 days as the limit time of chilled storage regarding consumers?perceptions.","palabras_clave":"MEAT INDUSTRY WASTE|NATURAL OCCURRING ANTIOXIDANTS|LIPID OXIDATION |HEALTHIER MEAT  COMMODITIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4712,"idioma_id":4,"titulo":"A new Upper Cretaceous titanosaur nesting site from La Rioja (NW Argentina), with implications for titanosaur nesting strategies","resumen":"Cretaceous titanosaur nesting sites are currently known only from Europe, Asia and South America. In the latter, only the Auca Mahuevo and Sanagasta nesting sites have been confidently assigned to this clade of sauropod dinosaurs. Here we report the discovery of the first eggs and egg clutches found at Tama, a new Upper Cretaceous fossilif- erous locality in the Los Llanos Formation, Sierra de Los Lla- nos (La Rioja, NW Argentina). At least five egg clutches, several partially preserved, isolated eggs and many eggshell fragments were discovered in a single outcrop of a sandstone horizon which represents a cumulative palaeosol profile. Although the mechanical and digital preparation of eggs did not reveal any embryonic remains in ovo, the morphology of the eggs and eggshells closely matches that of titanosaur eggs and eggshells found worldwide. The morphology and spatial grouping of the titanosaur eggs from Tama, along withgeological observations support a burrow-nesting strategy for these dinosaurs. Although the Sanagasta and Tama eggs were found in the same stratigraphical unit and share several morphological characters, they clearly differ in shell thickness and egg size. This, coupled with the interpretation of different sedimentary contexts for these nesting sites, strongly suggests that at least two different titanosaur species nested in La Rioja during the Late Cretaceous, using different nest- ing strategies. The occurrence of this new titanosaur nesting site in a semiarid palaeoenvironment represents an interesting case study for the reproductive biology of the titanosaur dinosaurs, particularly their labile nesting behaviour.","palabras_clave":"Tama|dinosaur eggs|burrow-nesting|nesting behaviour"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4713,"idioma_id":4,"titulo":"Genotyping approach for non-invasive fetal RHD detection in an admixed population","resumen":"Background: non-invasive fetal RHD genotyping can predict hemolytic disease of the fetus and the newborn in pregnancies with anti-D alloantibodies and also avoid antenatal anti-D prophylaxis in pregnant women carrying an RHD negative fetus. Considering that Argentineans? genetic background is the result of generations of intermixing between several ethnic groups, we evaluated the diagnostic performance of a non-invasive fetal RHD determination strategy to guide targeted antenatal RhD immunoprophylaxis. This algorithm is based on the analysis of four regions of the RHD gene in cell free fetal DNA in maternal plasma and maternal and paternal RHD genotyping.Materials and methods: DNA from 298 serologically D negative pregnant women between 19-28 weeks? gestation were RHD genotyped. Fetal RHD status by real time PCR was determined in 296 maternal plasma samples. In particular cases, RHD&#936; and RHD-CE-Ds alleles were investigated in paternal DNA. Umbilical cord blood was collected at birth and serological and molecular studies were performed.Results: among the 298 maternal samples, 288 were D-\/RHD- and 10 D-\/RHD+ (2 RHD*DAR; 5 RHD-CE-Ds; 3 RHD&#936;). Plasma from RHD*DAR carriers was not analysed. Real time PCR showed 210 RHD+ and 78 RHD- fetuses and 8 inconclusive results. Regarding this latter group, paternal molecular studies were useful to report a RHD negative status in 5 fetuses while only 3 remained inconclusive. All the results, except one false positive due to a silent allele [RHD(581insG)], agreed with the neonatal typing performed in cord blood.Discussion: the protocol used for noninvasive prenatal RHD genotyping proved to be suitable to determine fetal RHD status in our admixed population. The knowledge of the genetic background of the population under study and maternal and paternal molecular analysis allows reducing the number of inconclusive fetal RHD status results.","palabras_clave":"Prenatal diagnostic |RHD genotyping |immunoprophylaxis |admixed population"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4714,"idioma_id":5,"titulo":"Perfil lipídico y estabilidad oxidativa de hamburguesas de carne con agregado de aceite de soja y antioxidantes de origen natural","resumen":"Se evalúa la mejora del perfil lipídicoen hamburguesas de carne con agregadode aceite de soja como fuente de ácidos grasossaludables, y la estabilidad oxidativa del productoen presencia de antioxidantes de origen naturalque puedan reemplazar a antioxidantes sintéticos.Además se valora el cambio de color producidopor el agregado de los diferentes componentes.","palabras_clave":"ANTIOXIDANTES |ACEITES VEGETALES|ALIMENTOS CÁRNICOS|ÁCIDOS GRASOS N-3"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4716,"idioma_id":4,"titulo":"Intermolecular perturbation in the Self-assembly of melamine","resumen":"A theoretical study has been conducted on a set of supramolecular complexes based on 1,3,5-triazine-2,4,6-triamine, or melamine (M), with cyanuric acid (CA), trithiocyanuric acid (TCA), and two mono-substituted derivatives of CA with chlorine (CACl) and bromine (CABr). The study was carried out on 12 complexes, M\/(CA)n, M\/(TCA)n, M\/(CACl)n, M\/(CABr)n, with n = 1, 2 and 3, by the density functional theory employing the &#969;-B97XD functional with the 6-11++G(d,p) basis set. Information about the intermolecular perturbation over M and the interactions that drive the self-assembly of these species has been obtained from the quantum theory of atoms in molecules and a natural bond orbital analysis. The harmonic oscillator model of aromaticity, the para-delocalization index, the flctuation aromatic index, and two electron charge density descriptors were used to evaluate the aromaticity of M in each complex. Results show that the hydrogen and halogen (XBs) bond interactions, which direct the self-assembly process in these complexes, are anti-cooperative. Binding energies decrease in the following order: M\/(CA)n > M\/(TCA)n > M\/(CABr)n > M\/(CACl)n (for all values of n). Brominated CA arises as a potential compound toself-assembly with M via XBs.","palabras_clave":"Cyanuric acid|Supramolecular|Molecular building blocks|Hydrogen bond|Halogen bond"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4717,"idioma_id":4,"titulo":"Draft Genome Sequence of Acinetobacter bereziniae HPC229, a Carbapenem-Resistant Clinical Strain from Argentina Harboring blaNDM-1","resumen":"We report here the draft genome sequence of an NDM-1-producing Acinetobacter bereziniae clinical strain, HPC229. This strain harbors both plasmid and chromosomal resistance determinants toward different &#946;-lactams and aminoglycosides as well as several types of multidrug efflux pumps, most likely representing an adaptation strategy for survival under different environments.","palabras_clave":"DRAFT GENOME|ACINETOBACTER BEREZINIAE|CARBAPENEM RESISTANT |NDM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4718,"idioma_id":4,"titulo":"Clomipramine kills Trypanosoma brucei by apoptosis","resumen":"Drug repositioning, i.e. use of existing medicals to treat a different illness, is especially rewarding for neglected tropical diseases (NTD), since in this field the pharmaceutical industry is rather reluctant to spend vast investments for drug development. NTDs afflict primarily poor populations in under-developed countries, which minimizes financial profit. Here we investigated the trypanocidal effect of clomipramine, a commercial antipsychotic drug, on Trypanosoma brucei. The data showed that this drug killed the parasite with an IC50 of about 5 &#956;M. Analysis of the involved cell death mechanism revealed furthermore an initial autophagic stress response and finally the induction of apoptosis. The latter was substantiated by a set of respective markers such as phosphatidylserine exposition, DNA degradation, loss of the inner mitochondrial membrane potential and characteristic morphological changes. Clomipramine was described as a trypanothione inhibitor, but as judged from our results it also showed DNA binding capacities and induced substantial morphological changes. We thus consider it likely that the drug induces a multifold adverse interaction with the parasite?s physiology and induces stress in a way that trypanosomes cannot cope with.","palabras_clave":"Apoptosis |Clomipramine|Sleeping sickness"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4719,"idioma_id":4,"titulo":"Analysis of the CFO successive interference cancellation for the OFDMA uplink","resumen":"The uplink of orthogonal frequency division multiple access (OFDMA)or single-carrier frequency division multiple access (SC-FDMA) suffers multipleaccess interference when carrier frequency offset (CFO) is not properly estimatedand compensated. In particular, multicarrier uplink CFO compensation is highlycomplex due to the multiuser context. Successive interference cancellation algorithmsare effectively employed to compensate for the CFO, where the interferenceproduced by each user is handled sequentially through a series of iterations. Themain contribution of this work is the analysis of the CFO compensation performanceof efficient successive cancellation algorithms. We study the mean squaresymbol error, and derive a useful upper-bound of the compensation technique performanceat convergence. This result extends the general convergence results forthe space-alternating generalized expectation-maximization algorithm in the CFOcompensation scenario. Finally, we validate the analysis with numerical simulations.","palabras_clave":"OFDM|SC-FDMA|carrier frequency offset|multiuser interference"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4720,"idioma_id":4,"titulo":"Draft Genome Sequence of Photorhabdus luminescens Strain DSPV002N Isolated from Santa Fe, Argentina","resumen":"Here, we report the draft genome sequence of Photorhabdus luminescens strain DSPV002N, which consists of 177 contig sequences accounting for 5,518,143 bp, with a GC content of 42.3% and 4,701 predicted protein-coding genes (CDSs). From these, 27 CDSs exhibited significant similarity with insecticidal toxin proteins from Photorhabdus luminescens subsp. laumondii TT01.","palabras_clave":"PHOTORHABDUS LUMINESCENS|ENTOMOPATHOGENIC NEMATODES|HETERORHABDITIS BACTERIOPHORA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4721,"idioma_id":5,"titulo":"América Latina como problema y como solución: Robert Triffin, Daniel Cosío Villegas, Víctor Urquidi y Raúl Prebisch antes del Manifiesto Latinoamericano (1944-1946)","resumen":"Robert Triffin (1911-1993), Daniel Cosío Villegas (1898-1976) y Raúl Prebisch (1901-1986) han sido objeto de investigaciones que los tienen a cada uno de ellos como protagonistas. Sin embargo, analizarlos de manera conjunta como aquí proponemos nos permite un abordaje más comprehensivo de, en principio, un problema: la construcción de un debate, sobre un tema considerado de una región en particular, el de la economía Latinoamericana.  Esta aproximación implica pensar la causalidad de cómo y porqué organizaron este equipo de discusión y gestión de política económica, de debate intelectual sobre una región que era a la vez objeto de una teoría económica específica que discutía con, e incidía de forma también disímil en, las políticas domésticas de México, Norteamérica y Argentina.  Los tres compartieron una serie de encuentros propiciados por el Banco de México en 1944 y en 1946, provenían de realidades nacionales muy diferentes y sus trayectorias también fueron disímiles pero coincidieron en una serie de preguntas y posibles respuestas sobre la ?periferia? y el ?subdesarrollo? latinoamericano. En este trabajo inicial -porque pensamos que es realmente un primer acercamiento a un problema muchísimo más vasto- nos proponemos en primer lugar recuperar las trayectorias individuales de cada uno, para entender cómo se produjo la confluencia, interés, admiración y trabajo en conjunto de Prebisch, Triffin y Cosío Villegas (que implicó la participación de otros que en este primer acercamiento no tendremos en cuenta). Nos interesa especialmente la concepción que cada uno de ellos presentó sobre la región latinoamericana, y la forma en que esa idea guió prácticas político- económicas en los años bajo estudio en este trabajo, especialmente referidas a la cuestión de la banca central. Junto a esto, el objetivo es comenzar a recorrer una serie de asesorías económicas que rompieron con el modelo preexistente donde un experto salía por el mundo a sugerir cambios similares en toda la región, para reemplazarlo por un experto con mayor vínculo con las particularidades de los países de América Latina.  El primer encuentro en México que aquí analizamos tuvo lugar entre enero y marzo de 1944, el segundo en 1946. El momento no es casual. El contexto de la guerra imponía mundialmente la necesidad de pensar el mundo económico futuro. En 1944, además, en el mes de julio tuvieron lugar las reuniones de la Conferencia Bretton Woods (donde coincidieron Cosío Villegas y Triffin, por ejemplo), en la que en buena medida se selló el destino económico de posguerra. Ese año, también, tendrá existencia formal el Fondo Monetario Internacional, y bajo ese mismo espíritu se creará en 1948 la Comisión Económica para América Latina a expensas del Consejo Económico y Social de las Naciones Unidas, de igual modo que el Consejo lo había hecho en Europa, y en Asia y Lejano Oriente. Se trataba de crear un orden nuevo para un mundo considerado también ?nuevo?.","palabras_clave":"LATINOAMERICANISMO|RAUL PREBISCH|DANIEL COSIO VILLEGAS|ROBERT TRIFFIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4722,"idioma_id":4,"titulo":"A mixture of kefir-isolated bacteria and yeasts inhibits Shigella flexneri invasion and modulates the pro-inflammatory response on intestinal epithelial cells","resumen":"The aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (MM) to protect intestinal epithelial cells against Shigella flexneri invasion, as well as to analyse the effect on pro-inflammatory response elicited by this pathogen. A significant decrease in S. flexneri strain 72 invasion was observed on both HT-29 and Caco-2 cells pre-incubated with MM. Pre-incubation with the individual strains Saccharomyces cerevisiae CIDCA 8112 or Lactococcus lactis subsp. lactis CIDCA 8221 also reduced the internalisation of S. flexneri into HT-29 cells although in a lesser extent than MM. Interestingly, Lactobacillus plantarum CIDCA 83114 exerted a protective effect on the invasion of Caco-2 and HT-29 cells by S. flexneri. Regarding the pro-inflammatory response on HT-29 cells, S. flexneri infection induced a significant activation of the expression of interleukin 8 (IL-8), chemokine (C-C motif) ligand 20 (CCL20) and tumour necrosis factor alpha (TNF-&#945;) encoding genes (P<0.05), whereas incubation of cells with MM did not induce the expression of any of the mediators assessed. Interestingly, pre-incubation of HT-29 monolayer with MM produced an inhibition of S. flexneri-induced IL-8, CCL20 and TNF-&#945; mRNA expression. In order to gain insight on the effect of MM (or the individual strains) on this pro-inflammatory response, a series of experiments using a HT-29-NF-&#954;B-hrGFP reporter system were performed. Pre-incubation of HT-29-NF-&#954;B-hrGFP cells with MM significantly dampened Shigella-induced activation. Our results showed that the contribution of yeast strain Kluyveromyces marxianus CIDCA 8154 seems to be crucial in the observed effect. In conclusion, results presented in this study demonstrate that pre-treatment with a microbial mixture containing bacteria and yeasts isolated from kefir, resulted in inhibition of S. flexneri internalisation into human intestinal epithelial cells, along with the inhibition of the signalling via NF-&#954;B that in turn led to the attenuation of the inflammatory response","palabras_clave":"kefir microorganisms|Shiguella flexnerii|proinflamatory response"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4723,"idioma_id":4,"titulo":"Selected Lactobacillus strains isolated from sugary and milk kefir reduce Salmonella infection of epithelial cells in vitro","resumen":"AbstractThe isolation of potentially probiotic strains and the subsequent study of their properties are very important steps to gain insight in the health benefits ascribed to sugary and milk kefir. The aim of the present study was to characterise fifteen Lactobacillus strains isolated from these beverages by determining some surface properties and their ability to antagonise enterocyte cell damage after Salmonella infection in vitro. Lactobacillus surface properties were determined by hydrophobicity, autoaggregation, and coaggregation assays with Salmonella. In addition, lactobacilli adhesion to Caco-2\/TC-7 cells and the effect on Salmonella invasion were evaluated. Finally, the disassembly of F-actin cytoskeleton on intestinal epithelial cells was assayed in vitro when Salmonella infection was performed in the presence of selected Lactobacillus strains. Ten out of the 15 strains showed a high adhesion capacity to Caco-2\/TC-7 cells. Most of the strains were hydrophilic and non-autoaggregating. Strains isolated from sugary kefir were non-coaggregating with Salmonella while strains L.paracasei CIDCA 83120, 83121, 83123, 83124, 8339, 83102 isolated from milk kefir were able to coaggregate after 1 hour. L. paracasei CIDCA 8339 and L. kefiri CIDCA 83102 were able to diminish Salmonella invasion to the enterocytes. An antagonistic effect on cytoskeleton disruption elicited by the pathogen was also demonstrated. Our results suggest that both strains isolated from milk kefir could be considered as appropriate probiotic candidates.","palabras_clave":"COAGREGATION|AUTOAGREGATION|HYDROPHOBICITY|ADHESION|INVASION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4724,"idioma_id":4,"titulo":"Experimental set up for the irradiation of biological samples and nuclear track detectors with UV C","resumen":"Aim: In this work we present a methodology to produce an ?imprint? of cells cultivated on a polycarbonate detector by exposure of the detector to UV C radiation.Background: The distribution and concentration of10B atoms in tissue samples coming from BNCT (Boron Neutron Capture Therapy) protocols can be determined through the quantification and analysis of the tracks forming its autoradiography image on a nuclear track detector. The location of boron atoms in the cell structure could be known more accurately by the simultaneous observation of the nuclear tracks and the sample image on the detector.Materials and Methods: A UV C irradiator was constructed. The irradiance was measured along the lamp direction and at different distances. Melanoma cells were cultured on poly-carbonate foils, incubated with borophenylalanine, irradiated with thermal neutrons and exposed to UV C radiation. The samples were chemically attacked with a KOH solution.Results: A uniform irradiation field was established to expose the detector foils to UV C light.Cells could be seeded on the polycarbonate surface. Both imprints from cells and nuclear tracks were obtained after chemical etching.","palabras_clave":"BNCT|UV-C|POLYCARBONATE DETECTORS|CELL IMPRINTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4725,"idioma_id":5,"titulo":"Aderezos a base de vegetales","resumen":"Artículo completo","palabras_clave":"pastas vegetales|formulación |aceptació"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4726,"idioma_id":4,"titulo":"Remote sensing of Nitrogen status in wheat by radiometric response of its canopy","resumen":"xx","palabras_clave":"remote sensing|nitrogen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4727,"idioma_id":5,"titulo":"LA EXPLOTACIÓN DEL HUEMUL (HIPPOCAMELUS BISULCUS, MOLINA 1782) EN LA PATAGONIA A LO LARGO DEL HOLOCENO","resumen":"El objetivo de este trabajo es evaluar la explotación del huemul (Hippocamelus bisulcus) por parte de las poblaciones cazadoras en cuatro contextos ambientales patagónicos: el bosque andino, el ecotono bosqueestepa, el bosque asociado al litoral marino y la estepa. Específicamente, buscamos establecer si existierondiferencias en el lugar que ocupó este cérvido respecto de las otras especies utilizadas por estos cazadores, así como también las formas en que fue aprovechado a lo largo del Holoceno en cada uno de estos contextos.Utilizamos una base de datos de 73 sitios arqueológicos de Argentina y Chile que comprenden 112 registros con restos de la especie en sus secuencias estratigráficas. Los resultados muestran que la importancia del huemulen la subsistencia dependió de la estructura de recursos de cada ambiente y, por lo tanto, consideramos que el papel del huemul estuvo ligado a la historia de uso de los diferentes ambientes patagónicos durante el Holoceno.","palabras_clave":"HIPPOCAMELUS BISULCUS|ZOOARQUEOLOGÍA|CAZADORES-RECOLECTORES|PATAGONIA|HOLOCENO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4728,"idioma_id":4,"titulo":"Knowledge of the General Community in Cordoba, Argentina, on Human Papilloma Virus Infection and its Prevention","resumen":"Background: Most studies of human papilloma virus (HPV) are aimed at the natural history of the infectionand its relation to cancer; however, there are few studies to assess knowledge of the general population. Ouraim was analyze the degree of knowledge of Argentinians about HPV infection and its prevention. Materialsand Methods: We conducted a voluntary, anonymous and non-binding survey with 27 multiple-choice items, intwelve private and public establishments, selected to include a broad population in terms of education, age andgender. The survey consisted of three sections: individual characteristics of the volunteer, HPV infection basicknowledge, its prevention and the virus relationship with other cancers. Results: One thousand two hundredninety seven volunteers aged 18 to 80 participated. The total number of correct answers was 45.1%. The correctanswers for relationship HPV and cervical cancer was 62.1%. Almost 55% did not know about types of HPV thatthe vaccines for protection. Statistical analysis showed that women, single people, workers, the better educated,those who have had a STDs or HPV and receiving information through medical or educational establishmentshad greater knowledge of the topic. Only 0.2% of participants answered all questions correctly. Conclusions:Knowledge plays an important role in health care and the deficiency found in our population could influencethe success of the measures taken in the fight against cervical cancer. In this regard, we believe it would beappropriate, not only to emphasize early diagnosis and vaccine implementation, but also incorporate newcommunication strategies, facilitating reception of accurate and precise information by all strata of society.","palabras_clave":"HPV"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4729,"idioma_id":4,"titulo":"Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum","resumen":"The role of endophytic communities of seeds is still poorly characterised. The purpose of this work was to survey the presence of bacterial endophytes in the seeds of a commercial wheat cultivar widely sown in Argentina and to look for plant growth promotion features and biocontrol abilities against Fusarium graminearum among them. Six isolates were obtained from wheat seeds following a culture-dependent protocol. Four isolates were assignated to Paenibacillus genus according to their 16S rRNA sequencing. The only gammaproteobacteria isolated, presumably an Enterobactereaceae of Pantoea genus, was particularly active as IAA and siderophore producer, and also solubilised phosphate and was the only one that grew on N-free medium. Several of these isolates demonstrated ability to restrain F. graminearum growth on dual culture and in a bioassay using barley and wheat kernels. An outstanding ability to form biofilm on an inert surface was corroborated for those Paenibacillus which displayed greater biocontrol of F. graminearum, and the inoculation with one of these isolates in combination with the Pantoea isolate resulted in greater chlorophyll content in barley seedlings. Our results show a significant ecological potential of some components of the wheat seed endophytic community.","palabras_clave":"barley|bioassays|fusarium|wheat endophytes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4730,"idioma_id":5,"titulo":"GEOLOGÍA DEL SECTOR ORIENTAL DEL COMPLEJO PLUTÓNICO VOLCÁNICO CURACO (TRIÁSICO SUPERIOR), RÍO NEGRO","resumen":"El Complejo plutónico volcánico Curaco (Triásico Superior), se ubica al NE de la localidad de El Cuy, Río Negro, y su sector oriental está constituido por monzogranitos, escasa granodiorita y diques máficos a félsicos, y en menor proporción por sienita, monzonita y diorita. Los monzogranitos son biotíticos con textura porfírica, equigranular y granofírica-gráfica, y exhibencaracterísticas de emplazamiento sub-volcánico. Estas tres facies monzograníticas tienen contactos tectónicos a través de fajas de cizalla frágil-dúctil, y sólo se mapeó un contacto transicional entre la facies porfírica y equigranular. La granodiorita se presenta dentro de las facies monzograníticas como enclaves de contactos netos. Diques sin-magmáticos aplítico-pegmatíticosson consanguíneos del monzogranito porfírico. El análisis modal QAP sugiere una evolución monzogranítica que se enriquece progresivamente en cuarzo hacia los diques consanguíneos, y otra granodiorítica que evoluciona en forma paralela. El Complejo Curaco está truncado por la faja de cizalla dúctil Brusain de orientación NO-SE, compuesta por granitos milonitizados en el centro, y milonitas y ultramilonitas de grado medio en sus bordes. Diques graníticos sin-orogénicos intruyen en forma concordante a la cizalla. Un dato Rb-Sr de 192 Ma indicaría que la edad del cizallamiento dúctil se ubica en el Jurásico inferior.Diques máficos intruyen al monzogranito, mientras que diques riolíticos son post-orogénicos respecto al cizallamiento dúctil y contienen enclaves de diques máficos. Finalmente, fallas transcurrentes E-O de desplazamiento dextral truncan a todo el conjunto. Las características petrológicas y estructurales del complejo Curaco indican que podría correlacionarse con el Granito Calvo del Complejo plutónico volcánico Dos Lomas.","palabras_clave":"Petrologia|Granitoide|Milonita|Macizo norpatagonico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4731,"idioma_id":4,"titulo":"?SPECTROSCOPIC AND THERMODYNAMIC STUDY OF CHRYSIN AND QUERCETIN COMPLEXES WITH Cu(II)?","resumen":"Chrysin and Quercetin are natural polyphenolic compounds which present benefitsin human health because of their biological properties. These flavonoids also have the capacity to complex a wide variety of metallic ions which could affect their bioactivity. In order to assay the complexant capacity of flavonoids, the metallic ion Cu(II) was selected. Formation of two flavonoids-Cu(II) complexes in ethanolic solutions were studied and both complexes presented 2:1 L:M stoichiometries. The apparent formation constants for Quercetin-Cu(II) are higher than those of Chrysin-Cu(II) ones, suggesting that Quercetin may act as a better analytical reagent than Chrysin. A molecular modeling analysis was performed in order to gain insight into the spectroscopic properties of the complexes.","palabras_clave":"QUERCETIN|CRHYSIN|CU(II)|METALLIC COMPLEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4732,"idioma_id":4,"titulo":"Genetic diversity and conservation status of managed vicun?a (Vicugna vicugna) populations in Argentina","resumen":"The vicun?a (Vicugna vicugna) was indiscriminatelyhunted for more than 400 years and, by the end of1960s, it was seriously endangered. At that time, a captivebreeding program was initiated in Argentina by theNational Institute of Agricultural Technology (INTA) withthe aim of preserving the species. Nowadays, vicun?as aremanaged in captivity and in the wild to obtain their valuablefiber. The current genetic status of Argentinean vicun?apopulations is virtually unknown. Using mitochondrialDNA and microsatellite markers, we assessed levels ofgenetic diversity of vicun?a populations managed in thewild and compared it with a captive population fromINTA. Furthermore, we examined levels of genetic structureand evidence for historical bottlenecks. Overall, allpopulations revealed high genetic variability with no signsof inbreeding. Levels of genetic diversity between captiveand wild populations were not significantly different,although the captive population showed the lowest estimatesof allelic richness, number of mitochondrial haplotypes,and haplotype diversity. Significant geneticdifferentiation at microsatellite markers was found betweenfree-living populations from Jujuy and Catamarca provinces.Moreover, microsatellite data also revealed geneticstructure within the Catamarca management area. Geneticsignatures of past bottlenecks were detected in wild populationsby the Garza Williamson test. Results from thisstudy are discussed in relation to the conservation andmanagement of the species.","palabras_clave":"vicuña|conservation|genetic diversity|bottlenecks"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4733,"idioma_id":4,"titulo":"Asymmetric Cancer Hallmarks in Breast Tumors on Different Sides of the Body","resumen":"During the last decades it has been established that breast cancer arises through the accumulation of genetic and epigenetic alterations in different cancer related genes. These alterations confer the tumor oncogenic abilities, which can be resumed as cancer hallmarks (CH). The purpose of this study was to establish the methylation profile of CpG sites located in cancer genes in breast tumors so as to infer their potential impact on 6 CH: i.e. sustained proliferative signaling, evasion of growth suppressors, resistance to cell death, induction of angiogenesis, genome instability and invasion and metastasis. For 51 breast carcinomas, MS-MLPA derived-methylation profiles of 81 CpG sites were converted into 6 CH profiles. CH profiles distribution was tested by different statistical methods and correlated with clinical-pathological data. Unsupervised Hierarchical Cluster Analysis revealed that CH profiles segregate in two main groups (bootstrapping 90-100%), which correlate with breast laterality (p=0.05). For validating these observations, gene expression data was obtained by RealTime-PCR in a different cohort of 25 tumors and converted into CH profiles. This analyses confirmed the same clustering and a tendency of association with breast laterality (p=0.15). In silico analyses on gene expression data from TCGA Breast dataset from left and right breast tumors showed that they differed significantly when data was previously converted into CH profiles (p=0.033). We show here for the first time, that breast carcinomas arising on different sides of the body present differential cancer traits inferred from methylation and expression profiles. Our results indicate that by converting methylation or expression profiles in terms of Cancer Hallmarks, it would allow to uncover veiled associations with clinical features. These results contribute with a new finding to the better understanding of breast tumor behavior, and can moreover serve as proof of principle for other bilateral cancers like lung, testes or kidney.","palabras_clave":"cancer hallmarks|breast cancer|epigenetics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4734,"idioma_id":4,"titulo":"Characterization of a novel Kazal-type serine proteinase inhibitor of Arabidopsis thaliana","resumen":"Many different types of serine proteinase inhibitors have been involved in several kinds of plant physiological processes, including defense mechanisms against phytopathogens. Kazal-type serine proteinase inhibitors, which are included in the serine proteinase inhibitor family, are present in several organisms. These proteins play a regulatory role in processes that involve serine proteinases like trypsin, chymotrypsin, thrombin, elastase and\/or subtilisin. In the present work, we characterized two putative Kazal-type serine proteinase inhibitors from Arabidopsis thaliana, which have a single putative Kazal-type domain. The expression of these inhibitors is transiently induced in response to leaf infection by Botrytis cinerea, suggesting that they play some role in defense against pathogens. We also evaluated the inhibitory specificity of one of the Kazal-type serine proteinase inhibitors, which resulted to be induced during the local response to B. cinerea infection. The recombinant Kazal-type serine proteinase inhibitor displayed high specificity for elastase and subtilisin, but low specificity for trypsin, suggesting differences in its selectivity. In addition, this inhibitor exhibited a strong antifungal activity inhibiting the germination rate of B. cinerea conidia in vitro. Due to the important role of proteinase inhibitors in plant protection against pathogens and pests, the information about Kazal-type proteinase inhibitors described in the present work could contribute to improving current methods for plant protection against pathogens.","palabras_clave":"Kazal-type domain|Serine proteinase inhibitor|Arabidopsis thaliana|Botrytis cinerea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4735,"idioma_id":4,"titulo":"Inhibition of polyamine oxidase activity affects tumor development during the maize-Ustilago maydis interaction","resumen":"Ustilago maydis is a biotrophic plant pathogenic fungus that leads to tumor development in the aerial tissues of its host, Zea mays. These tumors are the result of cell hypertrophy and hyperplasia, and are accompanied by the reprograming of primary and secondary metabolism of infected plants. Up to now, little is known regarding key plant actors and their role in tumor development during the interaction with U. maydis. Polyamines are small aliphatic amines that regulate plant growth, development and stress responses. In a previous study, we found substantial increases of polyamine levels in tumors. In the present work, we describe the maize polyamine oxidase (PAO) gene family, its contribution to hydrogen peroxide (H2O2) production and its possible role in tumor development induced by U. maydis. Histochemical analysis revealed that chlorotic lesions and maize tumors induced by U. maydis accumulate H2O2 to significant levels. Maize plants inoculated with U. maydis and treated with the PAO inhibitor 1,8-diaminooctane exhibit a notable reduction of H2O2 accumulation in infected tissues and a significant drop in PAO activity. This treatment also reduced disease symptoms in infected plants. Finally, among six maize PAO genes only the ZmPAO1, which encodes an extracellular enzyme, is up-regulated in tumors. Our data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction.","palabras_clave":"Biotic stress|1,8-Diaminooctane|Hydrogen peroxide|Polyamine oxidase|plant tumors|Ustilago maydis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4736,"idioma_id":4,"titulo":"Salt effects on functional traits in model and in economically important Lotus species","resumen":"One of the most common stress sources for plants is NaCl-derived soil salinity. The genus Lotus comprises model and economically important species, which have been thoroughly studied regarding their physiological response to salinity. The leaf area ratio (LAR), the root length ratio (RLR) and their components (specific leaf area (SLA) and leaf mass fraction (LMF), and specific root length (SRL) and root mass fraction (RMF), respectively) may be affected by high salinity levels in soil. In this work we characterized L. tenuis, L. corniculatus, L. filicaulis, L. creticus, L. burtii and L. japonicus plants grown under different salt concentrations (0, 50, 100 and 150 mM Na Cl), on the base of their SLA, LMF, SRL and RMF by principal components analysis (PCA). We also assessed the effect of different salt concentrations on LAR and RLR in each species, and explored whether changes in these traits provide a fitness benefit to this group of plants. Salinity (150 mM NaCl) increased LAR in L. burtii and L. corniculatus, but it did not affect the LAR of remaining species. The highest salt concentration caused a decrease of the RLR in L. japonicus Gifu, whereas this trait was not affected by salinity in the remaining Lotus species. Changes in LAR and RLR observed would not be adaptive, according to the adaptiveness analysis performed, with the exception of SLA changes in L. corniculatus. The PCA analysis revealed that under favorable conditions, plants privilege surfaces for light and nutrients acquisition (SLA and SRL), whereas at higher salt concentrations (100 and 150 mM), plants favor carbon allocation to leaves and roots (LMF and RMF) in detriment of their surfaces. PCA analysis also showed that L. creticus plants subjected to saline treatment were distinguished from the remaining Lotus species. Based on our results and previous studies, we suggest that observed augmentated carbon partition to leaves and roots, could constitute a salt-alleviating mechanism by toxic ions dilution.","palabras_clave":"LOTUS SPP|SALINITY|ROOT MORPHOLOGY|LEAF AREA RATIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4737,"idioma_id":4,"titulo":"'Soluble' vs 'Insoluble' Prussian blue based catalysts: Influence on Fenton-type treatment","resumen":"The influence of the synthesis procedure of supported Prussian blue nanoparticles (PBNP) on their activity and stability as a Fenton-type catalyst is studied. Hence, two catalysts are synthesized by adsorbing onto a support of PBNP formed ex situ through the reaction between FeCl3 and K3Fe(CN)6 using H2O2 as reducing agent, and following different washing protocols. A third catalyst is prepared through a two-step impregnation process with FeCl3 and K4[Fe(CN)6] aqueous solutions. The catalysts are tested in the orange G Fenton-type oxidation. The fresh and used catalysts are characterized by BET surface area, SEM, EDS, TEM, M¨ossbauer spectroscopy, total iron content and UV-vis spectrophotometry. It is demonstrated that under the synthesis conditions employed, the ?insoluble? form of Prussian blue is promoted in the ex situ procedure, whereas the two-step impregnation process leads to the ?soluble? Prussian blue formation. The washing of the justprepared catalysts at the reaction temperature helps in eliminating the unreacted species. Those catalysts based on ?insoluble? Prussian blue nanoparticles exhibit better behaviour in terms of stability. Significant removals are attained (100% azo dye, 60% TOC), at pH ¼ 3, 343 K after thirteen successive cycles of 300 min. The best catalyst displays the smallest amount of total ?free? Fe leached without releasing PBNP, ferrocyanide or ferricyanide ions into the reaction media. Reversible adsorption?desorption of organic intermediates avoids the loss of activity due to blockage of sites and\/or pores.","palabras_clave":"Heterogeneous Fenton like reaction|Prussian Blue Nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4738,"idioma_id":4,"titulo":"In situ DRIFT study of Au-Ir\/ceria catalysts: activity and stability for CO oxidation","resumen":"Monometallic gold and iridium, and bimetallicgold?iridium on ceria catalysts were synthesized bydeposition?precipitation with urea. The activity of theseceria supported catalysts for CO oxidation was: Au?Ir  Au[Ir. The bimetallic catalyst was highly activeand stable during the carbon monoxide oxidation reaction,showing synergism between both metals. The catalystswere thoroughly characterized chemical and structurally.High-resolution electron microscopy coupled with energydispersive spectroscopy and X-ray photoelectron spectroscopyshowed evidences of iridium?gold intimacy.In situ infrared spectroscopy in the diffuse reflectancemode was used to investigate the reactivity of the activesites. Concentration-modulation excitation spectroscopyallowed selective identification of intermediates and?spectator? species. CO chemisorbed onto iridium sites(2096, 2060 and 2012 cm-1) was found inactive, whereascarbon monoxide on metallic gold sites (Au0?CO,2110 cm-1) exhibited reactivity for CO oxidation. In thebimetallic catalyst, new active sites (Aud-?CO, 2095 and2026 cm-1) together with Au0?CO species were observedmost likely produced by the isolation and stabilization ofgold atoms by iridium.","palabras_clave":"CO oxidation|Gold|Iridium|Ceria|DRIFT|Modulation excitation espectroscopy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4739,"idioma_id":4,"titulo":"The importance of manganese in the cytoplasmic maturation of cattle oocytes: blastocyst production improvement regardless of cumulus cells presence during in vitro maturation","resumen":"Adequate dietary intake of manganese (Mn) is required for normal reproductive performance in cattle. This study was carried out to investigate the effect of Mn during in vitro maturation of bovine cumulus?oocyte complexes (COC) on apoptosis of cumulus cells, cumulus expansion, and superoxide dismutase (SOD) activity in the COC. The role of cumulus cells on Mn transport and subsequent embryo development was also evaluated. Early apoptosis decreased in cumulus cells matured with Mn compared with medium alone. Cumulus expansion did not show differences in COC matured with or without Mn supplementation. SOD activity was higher in COC matured with 6 ng\/ml Mn thanwith 0 ng\/ml Mn. Cleavage rates were higher in COC and denuded oocytes co-cultured with cumulus cells, either with or without Mn added to in vitro maturation (IVM) medium. Regardless of the presence of cumulus cells during IVM, the blastocyst rates were higher when 6 ng\/ml Mn was supplemented into IVM medium compared with growth in medium alone. Blastocyst quality was enhanced when COC were matured in medium with Mn supplementation. The results of the present study indicated that Mn supplementation to IVM medium enhanced the ?health? of COC, and improved subsequent embryo development and embryo quality.","palabras_clave":"CUMULUS CELLS|CUMULUS EXPANSION|EMBRYO DEVELOPMENT|MANGANESE|SUPEROXIDE DISMUTASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4740,"idioma_id":4,"titulo":"Is seed hydration memory dependent on climate? Testing this hypothesis with Mexican and Argentinian cacti species","resumen":"Seed hydration memory has been observed in some cacti species, but it remains unclear how general this process is. Our hypothesis is that hydration memory of cacti seeds depends on the environmental conditions where the species occur. To test this we used seven species from the Argentinian Cordoba Mountains (mesic environment): Gymnocalycium capillense, Parodia mammulosa, Echinopsis candicans,Gymnocalycium bruchii, Gymnocalycium mostii, Gymnocalycium quehlianum and  Gymnocalycium monvillei, and two species from the Mexican Chihuahuan Desert (dry environment): Echinocactus platyacanthus and Ferocactus pilosus. Four hydration (hours)\/dehydration (days) treatments were applied: T1 ¼ 24 h\/5 days, T2 ¼ 3 consecutive cycles of 24 h\/5 days, T3 ¼ 72 h\/5 days and T4 ¼ untreated seeds. The response variables were final seed germination (%) and mean germination time (t50). The two Mexican species responded to at least one treatment by increasing their germination and decreasing their mean germination time. For the Argentinian species, only G. mostii increased its germination in T1 and T2 while t50 was reduced in three species after hydration-dehydration treatments. For G. monvillei the shortest germination time occurred in T2 and T3, and for G. capillense and G. quehlianum t50 was shortest in T3. Hydration memory seems more common in arid environments, but it is also present in more mesic ones.","palabras_clave":"memoria hidratacion|cactaceae|germinacion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4741,"idioma_id":5,"titulo":"Predictores cognitivos de la capacidad de planificación en niños de 6 y 7 años de edad","resumen":"La planificación implica la habilidad para identificar y organizar secuencias de acciones con el fin de alcanzar metas específicas. En la literatura se observan resultados dispares respecto cuales son los mejores predictores de esta capacidad en niños. El objetivo de este trabajo es identificar en qué medida la voluntad de control, el control inhibitorio perceptual, la memoria de trabajo y la inteligencia fluida explican tal capacidad en niños que inician la Educación Primaria Básica. La muestra estuvo conformada por 289 participantes de entre 6 y 7 años de edad. Los resultados indicaron que únicamente la inteligencia fluida y la memoria de trabajo se asociaban y explicaban la capacidad de planificar. Se discuten las implicaciones de estos resultados para el diseño de futuras investigaciones.","palabras_clave":"PLANIFICACIÓN|NIÑOS|VOLUNTAD DE CONTROL|MEMORIA DE TRABAJO|INTELIGENCIA FLUIDA|CONTROL INHIBITORIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4742,"idioma_id":4,"titulo":"Phylogenetic and molecular analyses of human parainfluenza type 3 virus in Buenos Aires, Argentina, between 2009 and 2013: The emergence of new genetic lineages","resumen":"Despite that human parainfluenza type 3 viruses (HPIV3) are one of the leading causes of acute lower respiratory tract infections in children under five, there is no licensed vaccine and there is limited current information on the molecular characteristics of regional and global circulating strains. The aim of this study was to describe the molecular characterization of HPIV3 circulating in Buenos Aires. We performed a genetic and phylogenetic analysis of the HN glycoprotein gene. Between 2009 and 2013, 124 HPIV3-positive samples taken from hospitalized pediatric patients were analyzed. Four new genetic lineages were described. Among them, C1c and C3d lineages showed local circulation patterns,whereas C3e and C3f comprised sequences from very distant countries. Despite the diversity of the described genotypes, C3a and C3d predominated over the others, the latter was present during the first years of the study and it was progressively replaced by C3a. Molecular analyses showed 28 non-synonymous substitutions; of these, 13were located in potentially predicted B-cell Epitopes. Taken together, the emergence of genetic lineages and the information of the molecular characteristics of HN protein may contribute to the general knowledge of HPIV3 molecular epidemiology for future vaccine development and antiviral therapies.","palabras_clave":"HUMAN_PARAINFLUENZA_VIRUS_3|GENOTYPES|SEASONALITY|EMERGING_LINEAGES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4743,"idioma_id":4,"titulo":"Sixteen years of evolution of human respiratory syncytial virus subgroup A in Buenos Aires, Argentina: GA2 the prevalent genotype through the years","resumen":"Human respiratory syncytial virus (HRSV) is the main viral cause of acute lowerrespiratory tract infections (LRTI) in children worldwide. In recent years, severalpreclinical trials with vaccine candidates have been reported. It is in this sense thatmolecular epidemiological studies become important. Understanding viral dispersionpatterns before and after the implementation of a vaccine can provide insight into theeffectiveness of the control strategies. In this work we analyzed the molecularepidemiology of HRSV-A over a period of sixteen years (1999-2014) in Buenos Aires.By bioinformatic tools we analyzed 169 sequences of the G glycoprotein gene fromhospitalized pediatric patients with LRTI. We found that GA2 was the most prevalentgenotype (73.35%). GA5 genotype co-circulated in our region until 2009 when it wasno longer detected, except in 2011. The recently globally emerging ON1 lineage witha 72-nt duplication increased its frequency to become the only lineage detected inBuenos Aires in 2014. By discrete phylogeographic analysis of global ON1 strains wecould determine that Panama could be the location of the MRCA dated June 20,2010; and this lineage could be introduced in Argentina from Spain in April, 2011.This analysis also showed temporary and geographical clustering of ON1 strainsobserved as phylogenetic clades with strains exclusively associated from a singlecountry, nevertheless among our 44 ON1 strains from three outbreaks (2012-2014)we could also detect posterior reintroductions and circulation from United States,Cuba, South Korea, and Spain. The continuous phylogeographic analysis of onesublineage of Argentine ON1 strains allowed us to establish that there could be alocal clustering of some strains even in neighborhoods. This work shows the potentialof this type of bioinformatic tools in the context of a future vaccine surveillancenetwork to trace the spread of new genetic lineages in human populations","palabras_clave":"HRSV|GENOTYPES|ON1 GENETIC LINEAGE|PHYLOGEOGRAPHY|CIRCULATION PATTERNS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4744,"idioma_id":4,"titulo":"Transcript profiling reveals that Cystein protease inhibitors are up-regulated in tuber sprouts after extended darkness","resumen":"Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the3 tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein4 levels. Potato tubers become a source of carbon and energy until sprouts are capable of independent growth.5 Transcript profiling of sprouts grown under continuous light or dark conditions was performed using the6 TIGR 10K EST Solanaceae microarray. The profiles analyzed show a core of highly expressed transcripts that7 are associated to the reactivation of growth. Under light conditions, the photosynthetic machinery was fully8 activated; the highest up-regulation was observed for the Rubisco activase (RCA), the glyceraldehyde-3-9 phosphate dehydrogenase (GAPDH) and the Photosystem II 22 kDa protein (CP22) genes, among others. On10 the other hand, sprouts exposed to continuous darkness elongate longer, and after extended darkness,11 synthesis of chloroplast components was repressed, the expression of proteases was reduced while genes12 encoding cystein protease inhibitors (CPIs) and metallocarboxypeptidase inhibitors (MPIs) were strongly13 induced. Northern blot and RT-PCR analysis confirmed that MPI levels correlated with the length of the dark14 period, however CPI expression was strong only after longer periods of darkness, suggesting a feedback-loop15 (regulation mechanism) in response to dark-induced senescence. Prevention of cystein protease activity in16 etiolated sprouts exposed to extended darkness could delay senescence until they emerge to light.","palabras_clave":"Solanum tuberosum  |Tuber sprouting|Protease inhibitors|TIGR 10K Microarrays"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4745,"idioma_id":4,"titulo":"Techno-morphological and use-wear analyses of gunflints from two Spanish colonial sites (Patagonia, Argentina)","resumen":"The aim of this paper is to present the results of the analysis of a gunflints sample from two colonial archaeological sites located in Patagonia (Argentina). The questions that guided the analysis related primarily to determine their origin and their functionality and use, as well as the possibility of recycling and reclamation after their discard. We perform techno-morphological, raw materials and a use-wear analysis. The results indicate that, given their form, the raw materials used, an important amount of edge scarring and evidences of contact with metal, all of the pieces were used as gunflints and are of European origin.","palabras_clave":"GUNFLINTS|PATAGONIA ARGENTINA|18TH CENTURY|TECHNO-MORPHOLOGICAL AND USE-WEAR ANALYSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4746,"idioma_id":4,"titulo":"Potential vectors of Xylella fastidiosa: a study of leafhoppers and treehoppers in citrus agroecosystems affected by Citrus Variegated Chlorosis","resumen":"This study investigated the predominant leafhopper and treehopper (Hemiptera: Auchenorrhyncha) species in CVC-affected citrus agroecosystems in Argentina, their seasonal fluctuation, and which of them are potential vectors of X. fastidiosa, using molecular methods for detection. More than 6,000 Auchenorrhyncha were collected from three citrus agroecosystems over a period of three years using yellow sticky traps and entomological net. Cicadellidae and Membracidae were the most abundant families. Of the 43 species identified, five were predominant in citrus orchards, and three were predominant in weeds surrounding citrus plants. All predominant species and another four non-predominant species were positive for X. fastidiosa by PCR and Real Time PCR methods. In a transmission assay, Dechacona missionum, Tapajosa rubromarginata and Cyphonia clavigera transmitted X. fastidiosa successfully. Scaphytopius bolivianus and Frequenamia spiniventris populations increased once (during the summer), possibly due to favorable weather conditions, and Bucephalogonia xanthophis, Molomea lineiceps and Tapajosa rubromarginata populations increased twice a year: once in summer and once in winter, coinciding with the increase in early citrus shoots (flush). Among the X. fastidiosa-positive species, those with the higher population densities during the sprouting period, where trees are highly susceptible to infection, must be considered as most relevant vectors of CVC in the citrus-growing areas in Argentina.","palabras_clave":"Cicadellidae, Membracidae|sweet orange, tangerine|PCR, TR-PCR|seasonal fluctuations, Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4747,"idioma_id":4,"titulo":"Description of the tadpole of Alsodes neuquensis Cei, 1976 and comparison with the sibling species A. gargola Gallardo, 1970","resumen":"This paper describes the external and buccal cavity morphology, chondrocranium, hyobranchial apparatus and cranial muscles of Alsodes neuquensis in comparison with the sibling species A. gargola. The tadpole of A. neuquensis presents a shorter and higher tail, and a relatively smaller oral disc than A. gargola. The external larval morphology of Alsodes may be characterised as follows: (i) tooth row formula 2(2)\/3(1), (ii) single row of marginal papillae with a wide rostral gap, (iii) single row of submarginal papillae in the mental region, (iv) both supra- and infraangular submarginal papillae present. The buccal features do not show marked intrageneric differences. The anterior and\/or posterior pair of infralabial papillae may be simple or forked. All known tadpoles present one prenarial papilla accompanied or not by few pustules, and the lateral ridges of floor can be three to penta-armed. The chondrocranial features shared by the species of the genus Alsodes are (i) tetrapartite cartilage suprarostralis, (ii) chondrified adrostral tissue mass, (iii) processus anterolateralis of the larval crista parotica well developed or larval processus oticus, and (iv) large processus pseudopterygoideus that may be either completely fused to the basi cranii (A. vanzolinii, A. verrucosus and some A. neuquensis) or fused only at the tip (A. barrioi, A. gargola and some A. neuquensis). Larval cranial muscles are for the first time described for the genus Alsodes. They do not show differences between both species studied herein.","palabras_clave":"BUCCAL CAVITY MORPHOLOGY|CRANIAL MUSCLES|CRANIAL SKELETON|EXTERNAL MORPHOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4748,"idioma_id":5,"titulo":"Geología, mineralogía y estructura de la Mina Acazoque (Pb-Ag-Zn), Puna Salteña","resumen":"Mina Acazoque, ubicada en la Puna Salteña (24 ° S), corresponde a un depósito epitermal de baja sulfuración relacionada con la actividad magmática Miocena. Es un yacimiento de Pb-Ag-Zn emplazado en rocas graníticas correspondientes al complejo Eruptivo Oire que posee una corrida de aproximadamente 500 metros y potencia media de 0,60 metros. La mineralización primaria está constituida por galena, calcopirita, pirita, tetraedrita y estannita en ganga de fluorita, baritina, adularia, calcita y cuarzo. La mineralización secundaria está representada por cerusita, covelina, calcosina, azurita, malaquita y limonita. La veta tiene un contacto neto con la roca de caja y muestra textura maciza variando a brechosa.Microsonda electronica de barrido fue usada en este estudio con el fin de determinar la química mineral de los minerales más abundantes en la veta. La composición química de los minerales analizados en la veta  son: adularia: K 0.92 Al 0.89 Si 3.09 O 8, baritina: Ba 0.97 Sr 0.03 SO4, calcita: Ca 1.87 Mn 0.01 (CO3)2, cerusita: Pb 1.47 Ca 0.01  CO3,  galena: Pb 0.97 S1. El control de la mineralización es estructural, emplazándose en planos de falla de rumbo N 310º e inclinaciones de 70º al SO con desplazamiento relativo de bloques oblicuo (normal-dextral). Nuestros datos indican que el emplazamiento de la mineralización fue sin-deformacional, relacionado con  la evolución  de la caldera del cerro Aguas Calientes (10.3 Ma). El campo de esfuerzos local determinado resulta coherente con el regional.","palabras_clave":"Acarzoque|Puna|Pb Ag|cinemática"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4749,"idioma_id":4,"titulo":"Biotransformation of dehydro-epi-androsterone by Aspergillus parasiticus: metabolic evidences of BVMO activity","resumen":"The research on the synthesis of steroids and its derivatives is of high interest due to their clinicalapplications. A particular focus is given to molecules bearing a D-ring lactone like testolactone becauseof its bioactivity. The Aspergillus genus has been used to perform steroid biotransformations since it offersa toolbox of redox enzymes. In this work, the use of growing cells of Aspergillus parasiticus to study thebioconversion of dehydro-epi-androsterone (DHEA) is described, emphasizing the metabolic steps leadingto D-ring lactonization products. It was observed that A. parasiticus is not only capable of transformingbicyclo[3.2.0]hept-2-en-6-one, the standard Baeyer-Villiger monooxygenase (BVMO) substrate, but alsoyielded testololactone and the homo-lactone 3b-hydroxy-17a-oxa-D-homoandrost-5-en-17-one fromDHEA. Moreover, the biocatalyst degraded the lateral chain of cortisone by an oxidative route suggestingthe action of a BVMO, thus providing enough metabolic evidences denoting the presence of BVMO activityin A. parasiticus. Furthermore, since excellent biotransformation rates were observed, A. parasiticus is apromising candidate for the production of bioactive lactone-based compounds of steroidal origin in largerscales","palabras_clave":"Steroids|Dehydro-epi-androsterone|Cortisone|Biotransformation|Aspergillus parasiticus|Baeyer- Villiger monooxygenases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4750,"idioma_id":4,"titulo":"Andean uplift drives diversification of the bothriurid scorpion genus Brachistosternus","resumen":"AimOne of the planet´s most imposing geomorphological features, the Andes, played an important role in the evolution of South America´s flora and fauna. The bothriurid scorpion genus Brachistosternus Pocock, 1893 comprises more than 40 species with high diversity and endemism in the Andes. The present contribution investigates the biogeographical history of this genus using molecular phylogenetics and dating, to determine the role of Andean uplift on the distribution and diversification of its species.LocationSouth America.MethodsA dated species tree was obtained for 55 putative species based on two nuclear and three mitochondrial gene loci. Ancestral ranges and biogeographical events were estimated on the species tree, diversification rates and rate shifts calculated, and areas with high phylogenetic diversity (PD) and evolutionary distinctiveness identified.ResultsBrachistosternus diversified at a steady rate during the main Andean uplift. The central Andean and western slope\/Pacific coastal biogeographical provinces played important roles as ancestral areas. Coastal areas of central Chile and southern Peru exhibit high levels of PD in Brachistosternus, suggesting they experienced a relatively long period of ecological stability, while the Andes continued to rise.Main conclusionsAndean uplift created new habitats and climate regimes, favouring speciation in genera such as Brachistosternus. Coastal areas to the west of the Andes continued to harbour older lineages while accommodating more recently diverged lineages from the nearby Andes.","palabras_clave":"Andes|aridification|Bothriuridae|historical biogeography"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4751,"idioma_id":5,"titulo":"Optimización de cultivos primarios de células de carcinoma renal de células claras como modelo ?in vitro? para estudios metabólicos y determinantes de progresión neoplásica","resumen":"El objetivo del presente estudio fue optimizar la implementación de cultivos primarios a partir de muestras de carcinoma renal de células claras (CRCC) para comprobar la conservación del fenotipo lipogénico contra cortes fijados del mismo origen.Se utilizaron muestras de pacientes con CRCC, evaluándose diversas metodologías y condiciones experimentales de digestión de muestras, adherencia y despegue celular, fenotipo lipogénico, potencial de clonación, proliferación y capacidad de migración. El mayor rendimiento y viabilidad celular se verificó mediante digestión con colagenasa. La adherencia inicial se logró a las 24 hs de incubación, utilizando placas plásticas de cultivo, recubiertas con colágeno comercial y gelatina 0,2% en la mayoría de las muestras analizadas (60% de los casos). Se obtuvieron monocapas, con potencial de migración, en un 40% de los casos, tras 5 ± 1 días de incubación. El promedio de subcultivos fue de 3 ± 1. Este estudio permitió estandarizar cultivos primarios de CRCC comprobándose la conservación de la fenotipia lipogénica, logrando de dicha manera una herramienta importante y útil para el estudio de la biología tumoral y el ensayo de nuevas terapéuticas.Palabras clave: Cultivos primarios tumorales; carcinoma renal; optimización técnica; lipogénesis.","palabras_clave":"OPTIMIZACION|CULTIVOS|CARCINOMA RENAL|PROLIFERACION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4752,"idioma_id":4,"titulo":"Random Amplified Polymorphic DNA PCR in the Teaching of Molecular Epidemiology","resumen":"In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak","palabras_clave":"Microorganismos|Técnicas moleculares|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4753,"idioma_id":5,"titulo":"¿Qué mueve a los estudiantes exitosos? Metas y motivaciones de universitarios en las modalidades presencial y distancia","resumen":"El inicio de la vida universitaria es un periodo difícil de transitar para los estudiantes, tanto en los estudios presenciales como a distancia. No obstante, muchos logran el rendimiento académico esperado. El objetivo de este artículo reside en analizar las metas y motivaciones de un grupo de estudiantes que logró regularizar las asignaturas previstas por el plan de estudios de las carreras que estaban cursando. Participaron del estudio 85 estudiantes de carreras de Ciencias Económicas dictadas en modalidad presencial y 14 sujetos que cursaban en la modalidad virtual. Los datos fueron recogidos mediante la administración de un cuestionario de autoinforme. Los resultados muestran prevalencia de metas de aprendizaje entre los sujetos del grupo presencial en tanto que entre los estudiantes de distancia la meta más destacada es la de evitar el fracaso. La discusión destaca la dimensión contextual de la motivación, sugiriendo acciones capaces de promover la motivación por el aprendizaje.","palabras_clave":"METAS|MOTIVACIÓN|RENDIMIENTO ACADÉMICO|EDUCACIÓN PRESENCIAL|EDUCACIÓN A DISTANCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4754,"idioma_id":4,"titulo":"Erythropoietin attenuates renal and pulmonary injury in polymicrobial induced-sepsis through EPO-R, VEGF and VEGF-R2 modulation","resumen":"Sepsis remains the most important cause of acute kidney injury (AKI) and acute lung injury (ALI) in critically ill patients. The cecal ligation and puncture (CLP) model in experimental mice reproduces most of the clinical features of sepsis. Erythropoietin (EPO) is a well-known cytoprotective multifunctional hormone, which exerts anti-inflammatory, anti-oxidant, anti-apoptotic and pro-angiogenic effects in several tissues. The aim of this study was to evaluate the underlying mechanisms of EPO protection through the expression of the EPO\/EPO receptor (EPO-R) and VEGF\/VEF-R2 systems in kidneys and lungs of mice undergoing CLP-induced sepsis.Male inbred Balb\/c mice were divided in three experimental groups: Sham, CLP, and CLP + EPO (3000 IU\/kg sc). Assessment of renal functional parameters, survival, histological examination, immunohistochemistry and\/or Western blottings of EPO-R, VEGF and VEGF-R2 were performed at 18 h post-surgery.Mice demonstrated AKI by elevation of serum creatinine and renal histologic damage. EPO treatment attenuates renal dysfunction and ameliorates kidney histopathologic changes. Additionally, EPO administration attenuates deleterious septic damage in renal cortex through the overexpression of EPO-R in tubular interstitial cells and the overexpression of the pair VEGF\/VEGF-R2.Similarly CLP- induced ALI, as evidenced by parenchymal lung histopathologic alterations, was ameliorated through pulmonary EPO-R, VEGF and VEGF-R2 over expression suggesting and improvement in endothelial survival and functionality.This study demonstrates that EPO exerts protective effects in kidneys and lungs in mice with CLP-induced sepsis through the expression of EPO-R and the regulation of the VEGF\/VEGF-R2 pair.","palabras_clave":"Cecal ligation and puncture (CLP)  |Acute lung injury (ALI)| Sepsis |Erythropoietin (EPO)|Acute kidney injury (AKI)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4755,"idioma_id":4,"titulo":"FASCIOLA HEPATICA: HISTOLOGICAL CHANGES IN THE SOMATIC AND REPRODUCTIVE TISSUES OF LIVER FLUKE FOLLOWING CLOSANTEL TREATMENT OF EXPERIMENTALLY INFECTED SHEEP","resumen":"Lambs infected with the Cullompton isolate of Fasciola hepatica were treated orally or subcutaneously with 10 mg\/kg of closantel at 16 weeks post-infection. Adult flukes were recovered from the liver of individual animals at 12 h, 24 h, or 36 h post-treatment. The flukes were processed for histological analysis. In general, degenerative changes in the reproductive and somatic tissues were progressive, and were most marked in flukes exposed to closantel in vivo for 36 h. However, flukes from a 12 h subcutaneously-treated lamb showed marked deterioration of the testis, possibly because a portion of the dose has been delivered intravenously. Fewer intact eggs were seen in the uterus of flukes exposed to closantel for longer times (whether administered subcutaneously or orally to the host).The most conspicuous closantel-induced effect in flukes from treated hosts was progressive damage to the tegumental syncytium. While the flukes from 24 h-treated hosts showed relatively minor damage to limited areas of the syncytium,towards the posterior end,the flukes from 36 h-treated hosts (and flukes from the lamb that putatively received intravenous dosage) had lost large areas of the surface syncytium from the posterior end and dorsal surface,although the syncytium over the anterior end and the anterior ventral surface was largely spared. In areas where the syncytium had sloughed, the underlying structures such as the vitelline follicles, gut profiles and testis profiles, showed marked degeneration and breakdown. Other changes included cell depletion and incipient apoptosis in the testis, ovary and vitelline follicles. This study establishes a model for histological changes in closantel-sensitive F. hepatica exposed to closantel in vivo, and may inform the development of a diagnostic test to supplement the faecal egg count reduction test and coproantigen reduction test for closantel resistance in fluke populations.","palabras_clave":"FASCIOLA|CLOSANTEL|FLUKE RESISTANT DIAGNOSIS|FLUKE HISTOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4756,"idioma_id":4,"titulo":"Using remotely sensed data to model suitable habitats for tree species in a desert environment","resumen":"Questions: Can the species?environment relationship be understood using currentremote sensing techniques? Can the derived indicators of remotely senseddata serve as a proxy for variables that affect habitat suitability of plant species?Which remote sensing predictors are best associated with woody species occurrence in a desert environment? How well do models with derived indicators of remotely sensed data predict the occurrence of these species? What are thepotential distributions of Ramorinoa girolae, Prosopis spp. and Bulnesia retama in the study area?Location: Ischigualasto Provincial Park, San Juan province, Argentina.Methods: We selected random field points from a Landsat 8 OLI to determinepresence\/absence of trees species.We calculated Brightness index (BI) using thesame image and used this index to calculate texture measures on a 3 9 3 movingwindow size. We used the following subset of texture measures: (1) firstorder:range, (2) second-order: mean, variance, contrast, entropy, secondmoment and correlation.We also calculated TopographicWetness Index (TWI),slope angle and slope aspect from Global Digital Elevation Model.Results and Conclusion: Second-order mean of BI had an important effect onthe occurrence of target trees species. TWI was an important variable for Prosopis spp. and B. retama, whereas slope angle was important for R. girolae and B. retama.In addition, the occurrence of R. girolae was affected by second-order varianceof BI and slope aspect; and the presence of B. retama was affected bysecond-order contrast of BI. All the variables that had important effects on theoccurrence of tree species provide environmental information about their different habitat requirements; therefore, our findings indicate that the remote sensing data are reliable to derive indicators of tree species presence in our study area.","palabras_clave":"REMOTE SENSING DATA|WOODLANDS|HABITAT SUITABILITY|DESERT ENVIRONMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4757,"idioma_id":4,"titulo":"Delta- pgm , a new live-attenuated vaccine against Brucella suis","resumen":"Brucellosis is one of the most widespread zoonosis in the world affecting many domestic and wild animals including bovines, goats, pigs and dogs. Each species of the Brucella genus has a particular tropism toward different mammals being the most relevant for human health Brucella abortus, Brucella melitensis and Brucella suis that infect bovines, goats\/camelids and swine respectively. Although for B. abortus and B. melitensis there are vaccines available, there is no efficient vaccine to protect swine from B. suis infection so far. We describe here the construction of a novel vaccine strain that confers excellent protection against B. suis in a mouse model of infection. This strain is a clean deletion of the phosphoglucomutase (pgm) gene that codes for a protein that catalyzes the conversion of glucose-6-P to glucose-1-P, which is used as a precursor for the biosynthesis of many polysaccharides. The Delta-pgm strain lacks a complete lipopolysaccharide, is unable to synthesize cyclic beta glucans and is sensitive to several detergents and Polymyxin B. We show that this strain replicates in cultured cells, is completely avirulent in the mouse model of infection but protects against a challenge of the virulent strain inducing the production of pro-inflammatory cytokines. This novel strain could be an excellent candidate for the control of swine brucellosis, a disease of emerging concern in many parts of the world.","palabras_clave":"BRUCELLA SUIS|LIVE VACCINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4758,"idioma_id":4,"titulo":"BigA is a novel adhesin of Brucella that mediates adhesion to epithelial cells","resumen":"Adhesion to cells is the initial step in the infectious cycle of basically all pathogenic bacteria, and to do so, microorganisms have evolved surface molecules that target different cellular receptors. Brucella is an intracellular pathogen that infects a wide range of mammals whose virulence is completely dependent on the capacity to replicate in phagocytes. Although much has been done to elucidate how Brucella multiplies in macrophages, we still do not understand how bacteria invade epithelial cells to perform a replicative cycle or what adhesion molecules are involved in the process. We report the identification in Brucella abortus of a novel adhesin that harbours a bacterial immunoglobulin-like domain and demonstrate that this protein is involved in the adhesion to polarized epithelial cells such as the Caco-2 and Madin-Darby canine kidney models targeting the bacteria to the cell-cell interaction membrane. While deletion of the gene significantly reduced adhesion, over-expression dramatically increased it. Addition of the recombinant protein to cells induced cytoskeleton rearrangements and showed that this adhesin targets proteins of the cell-cell interaction membrane in confluent cultures.","palabras_clave":"Brucella abortus|Adhesin|Cell-cell interaction|Cytoskeleton rearrangements"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4759,"idioma_id":4,"titulo":"VirJ is a Brucella Virulence Factor Involved in the Secretion of Type IV Secreted Substrates","resumen":"The VirB secretion apparatus in Brucella belongs to the type IV secretion systems present in many pathogenic bacteria and is absolutely necessary for the efficient evasion of the Brucella-containing vacuole from the phagocytic route in professional phagocytes. This system is responsible for the secretion of a plethora of effector proteins that alter the biology of the host cell and promote the intracellular replication process. Although many VirB substrates have been identified in Brucella, we still know very little about the secretion mechanism that mediates their translocation across the two membranes and the periplasmic space. In this manuscript, we describe the identification of a gene, virJ, that codes for a protein with periplasmic localization that is involved in the intracellular replication process and virulence in mice. Our analysis revealed that this protein is necessary for the secretion of at least two VirB substrates that have a periplasmic intermediate and that it directly interacts with them. We additionally show that VirJ also associates with the apparatus per se and that its absence affects the assembly of the complex. We hypothesize that VirJ is part of a secretion platform composed of the translocon and several secretion substrates and that it probably coordinates the proper assembly of this macromolecular complex.","palabras_clave":"BRUCELLA ABORTUS|VIRULENCE FACTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4760,"idioma_id":4,"titulo":"Influence of lake trophic conditions on the dominant mixotrophic algal assemblages","resumen":"Mixotrophic protists combine photo-autotrophy and phago-heterotrophy. Even though only in oligotrophic systems mixotrophs overcome strictly autotrophs, they are represented all along the trophic spectrum. We hypothesize that lakes with different chlorophyll a (Chla) and dissolved organic carbon (DOC) concentrations harbor different assem- blages of mixotrophs. We examined the composition of mixotrophs in 24 lakes in two Patagonian regions (Argentina), with a range of conditions of Chla and DOC. The categorization of a predominant type of nutrition in each mixotroph was based on published evidence. Additionally in four lakes we analyzed the bacterivory activity of mixotrophs by performing ingestion experiments. We found potentially mixotrophic taxa in all lakes, however the assemblages varied depending on their trophic-DOC features. Primarily heterotrophic mixotrophs (e.g. chryso- phytes) attained a higher proportion in oligotrophic lakes, declining towards higher trophic status. In contrast, pri- marily autotrophic mixotrophs (e.g. cryptophytes) dominated in eutrophic systems. Mixotrophs actively ingested bacteria in all experiments, however cell-specific grazing rates were higher in oligotrophic lakes. Mixotrophs accounted for more than half of total flagellate grazing in oligo-mesotrophic environments. Our results suggest that there is a replacement of mixotrophic taxa along a gradient of increasing trophic state, from primarily heterotrophic to primarily autotrophic mixotrophs, whereas their relative contribution to total flagellate grazing decreases.","palabras_clave":"mixotrophs|phytoplankton|lakes|trophic status|DOC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4762,"idioma_id":4,"titulo":"MiR-16 mediates trastuzumab and lapatinib response in ErbB-2-positive breast and gastric cancer via its novel targets CCNJ and FUBP1","resumen":"ErbB-2 amplification\/overexpression accounts for an aggressive breast cancer (BC) subtype (ErbB-2-positive). Enhanced ErbB-2 expression was also found in gastric cancer (GC) and has been correlated with poor clinical outcome. The ErbB-2-targeted therapies trastuzumab (TZ), a monoclonal antibody, and lapatinib, a tyrosine kinase inhibitor, have proved highly beneficial. However, resistance to such therapies remains a major clinical challenge. We here revealed a novel mechanism underlying the antiproliferative effects of both agents in ErbB-2-positive BC and GC. TZ and lapatinib ability to block extracellular signal-regulated kinases 1\/2 and phosphatidylinositol-3 kinase (PI3K)\/AKT in sensitive cells inhibits c-Myc activation, which results in upregulation of miR-16. Forced expression of miR-16 inhibited in vitro proliferation in BC and GC cells, both sensitive and resistant to TZ and lapatinib, as well as in a preclinical BC model resistant to these agents. This reveals miR-16 role as tumor suppressor in ErbB-2-positive BC and GC. Using genome-wide expression studies and miRNA target prediction algorithms, we identified cyclin J and far upstream element-binding protein 1 (FUBP1) as novel miR-16 targets, which mediate miR-16 antiproliferative effects. Supporting the clinical relevance of our results, we found that high levels of miR-16 and low or null FUBP1 expression correlate with TZ response in ErbB-2-positive primary BCs. These findings highlight a potential role of miR-16 and FUBP1 as biomarkers of sensitivity to TZ therapy. Furthermore, we revealed miR-16 as an innovative therapeutic agent for TZ- and lapatinib-resistant ErbB-2-positive BC and GC.Oncogene advance online publication, 9 May 2016; doi:10.1038\/onc.2016.151.","palabras_clave":"Trastuzumab|ErbB-2|Breast cancer|MIR-16"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4763,"idioma_id":4,"titulo":"Chemical Constituents and Acetylcholinesterase Inhibition of Senecio ventanensis Cabrera (Asteraceae)","resumen":"Chemical constituents and acetylcholinesterase inhibition was  investigated in both essential oil and dichloromethane  subextract  obtained from the aerial parts  of  Senecio ventanensis,  an  endemic  Asteraceae.  The chemical composition of the oil was determined by  GC and  GC-MS. The major constituents were  a-terpinene (12.2%), limonene (11.9%), a-humulene  (10.5%),  sabinene (9.1%), terpinolene (8.8%),  p-cymene (8.1%) and a-ocimene (7.3%).  The bioassay guided isolation of the constituents of the  active  dichloromethane subextract (IC50  = 361.6 µg\/mL) led us to the isolation of  two diastereoisomers of  a sesquiterpene endoperoxide 3,6-epidioxy-1,10-bisaboladiene (1 and 2), reported here for the first time in  S. ventanensis.  The identification was achieved by comprehensive analyses of its 1H and 13C NMR spectroscopic  data (including 2D experiments)  and mass spectrometric data. Their absolute configuration is proposed on the basis of AM1 calculations and NOESY experiments. This is the first time that compounds 1 and 2 have been separated as well as the first phytochemical investigation of S. ventanensis.","palabras_clave":"SENECIO VENTANENSIS|ESSENTIAL OIL|SESQUITERPENE ENDOPEROXIDE|ACETYLCHOLINESTERASE INHIBITION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4765,"idioma_id":4,"titulo":"Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media","resumen":"To deal with complex systems, microscopic and global approaches become of particular interest. Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar?Parisi?Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively. In both cases, the influence of a non-uniform distribution of the colony constituents was significant. These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells. For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated. For small N, the average radial front velocity and its change with time depend on MC concentration. MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities. Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains. For large N, colony spreading occurs at constant velocity. The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium. Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ. In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation. Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.","palabras_clave":"VERO CELL CULTURE|COLONY HETERNOGENEITIES|CELL MOTILITY|GROWTH MODELS|DYNAMIC SCALING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4766,"idioma_id":4,"titulo":"Adapting Prospective Structural Analysis to strengthen sustainable management and capacity building in Community-Based Natural Resource Management contexts","resumen":"Local communities collectively managing common pool resources can play an important role in sustainable management, but they often lack the skills and context-specific tools required for such management. The complex dynamics of social-ecological systems (SES), the need for management capacities, and communities? limited empowerment and participation skills present challenges for community-based natural resource management (CBNRM) strategies. We analyzed the applicability of prospective structural analysis (PSA), a strategic foresight tool, to support decision making and to foster sustainable management and capacity building in CBNRM contexts and the modifications necessary to use the tool in such contexts. By testing PSA in three SES in Colombia, Mexico, and Argentina, we gathered information regarding the potential of this tool and its adaptation requirements. The results suggest that the tool can be adapted to these contexts and contribute to fostering sustainable management and capacity building. It helped identify the systems? dynamics, thus increasing the communities? knowledge about their SES and informing the decision-making process. Additionally, it drove a learning process that both fostered empowerment and built participation skills. The process demanded both time and effort, and required external monitoring and facilitation, but community members could be trained to master it. Thus, we suggest that the PSA technique has the potential to strengthen CBNRM and that other initiatives could use it, but they must be aware of these requirements.(Thompson Scienfic (ISI) Expanded Science Citation Index; PAIS, CSA, DOAJ, entre otros. Factores de impacto: SJR: 1.93, H Index: 45, ISI 2010 impact factor: 3.310, 5 year impact factor: 4.644).","palabras_clave":"ARGENTINA|COLOMBIA|LATIN AMERICA|LOCAL KNOWLEDGE|MEXICO|PARTICIPATORY TECHNIQUES|SOCIAL-ECOLOGICAL SYSTEM|STRATEGY FORESIGHT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4767,"idioma_id":4,"titulo":"alfa-Hydroxyacids  accelerate the  Diels-Alder  reaction  of  dibutyl vinylboronate  with  cyclopentadiene: experimental  results and mechanistic insights","resumen":"We  have  found  that &#945;-hydroxyacids  accelerate  the Diels-Alder reaction  of  dibutyl  vinylboronate  with  cyclopentadiene.  When stoichiometric  quantities  are  used excellent  yields are  obtained, while catalytic activities are moderate. DFT calculations suggested that activation  of  the  dienophile  occurs  by  ligand  exchange  with both functionalities of the &#945;-hydroxyacid.","palabras_clave":"HYDROXYACID|DIELS-ALER|BORONATE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4768,"idioma_id":4,"titulo":"Broadband resonant cavity inside a two-dimensional sonic crystal","resumen":"A rectangular cavity inside a two-dimensional sonic crystal was theoretically and experimentally characterized by examining its response to a cylindrical source emitting narrow-band filtered noise bursts with central frequencies ranging from 2 to 12 kHz. A broadband intensity resonance was observed for frequencies within the full band-gap region of the sonic crystal (5.5?6.5 kHz). Unlike ordinary resonances, this broadband resonance depends on the reflection properties of the sonic crystal forming the surrounding walls rather than on the geometry of the cavity.","palabras_clave":"Composite material|Band-gap|Broadband resonance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4769,"idioma_id":4,"titulo":"Innate host habitat preference in the generalist parasitoid Diachasmimorpha longicaudata: functional significance and modifications through learning.","resumen":"Parasitoids searching for polyphagous herbivores can find their hosts in a variety of habitats. Under this scenario, chemical cues from the host habitat (not related to the host) represent poor indicators of host location. Hence, it is unlikely that naïve females show a strong response to host habitat cues, which would become important only if the parasitoids learn to associate such cues to the host presence. This concept does not consider that habitats can vary in profitability or host nutritional quality, which according to the optimal foraging theory and the preference-performance hypothesis (respectively) could shape the way in which parasitoids make use of chemical cues from the host habitat. We assessed innate preference in the fruit fly parasitoid Diachasmimorpha longicaudata among chemical cues from four host habitats (apple, fig, orange and peach) using a Y-tube olfactometer. Contrary to what was predicted, we found a hierarchic pattern of preference. The parasitism rate realized on these fruit species and the weight of the host correlates positively, to some extent, with the preference pattern, whereas preference did not correlate with survival and fecundity of the progeny. As expected for a parasitoid foraging for generalist hosts, habitat preference changed markedly depending on their previous experience and the abundance of hosts. These findings suggest that the pattern of preference for host habitats is attributable to differences in encounter rate and host quality. Host habitat preference seems to be, however, quite plastic and easily modified according to the information obtained during foraging.","palabras_clave":"Mosca de los frutos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4770,"idioma_id":4,"titulo":"Foraging behavior interactions between two non-native social wasps, Vespula germanica and V. vulgaris: implications for invasion success?","resumen":"Vespula vulgaris is an invasive scavenging social wasp that has very recently arrived in Patagonia (Argentina), a territory previously invaded -35 years earlier- by another wasp, V. germanica. Although V. vulgaris wasps possess features that could be instrumental in overcoming obstacles through several invasion stages, the presence of pre-established populations of V. germanica could affect their success. We studied the potential role played by V. germanica on the subsequent invasion process of V. vulgaris wasps in Patagonia by focusing on the foraging interaction between both species. This is because food searching and exploitation are likely to overlap strongly among Vespula wasps. We carried out choice tests where two types of baits were presented in a pairwise manner. We found experimental evidence supporting the hypothesis that V. germanica and V. vulgaris have asymmetrical response to baits with stimuli simulating the presences of each other. V. germanica avoided baits with either visual or actory cues indicating the V. vulgaris presence. However, V. vulgaris showed no preference between baits with or lacking V. germanica stimuli. These results suggest that the presence of an established population of V. germanica may not contribute to added biotic resistance to V. vulgaris invasion.","palabras_clave":"Avispas invasotas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4771,"idioma_id":4,"titulo":"Dispersal behaviour of yellowjacket (Vespula germanica) queens.","resumen":"Understanding the factors that affect animal dispersal behavior is important from both fundamental and applied perspectives. Dispersal can have clear evolutionary and ecological consequences, but for nonnative insect pests, dispersal capacity can also help to explain invasion success. Vespula germanica is a social wasp that, in the last century, has successfully invaded several regions of the world, showing one of the highest spread rates reported for a nonnative insect. In contrast with nonsocial wasps, in social species, queens are responsible for population redistribution and spread, as workers are sterile. For V. germanica, it has been observed that queen flight is limited to 2 distinct periods: early autumn, when new queens leave the nest to mate and find sheltered places in which to hibernate, and spring when new colonies are founded. Our aim was to study the flight behavior of V. germanica queens by focusing on the different periods in which dispersal occurs, characterizing as well the potential contribution of queen flight (i.e., distance) to the observed geographical spread. Our results suggest that the distances flown by nonoverwintered queens is greater than that flown by overwintered individuals, suggesting that the main queen dispersal events would occur before queens enter hibernation. This could relate to a behavioral trait of the queens to avoid the inbreeding with related drones. Additionally, given the short distances flown and remarkable geographical spread observed, we provide evidence showing that queen dispersal by flight is likely to contribute proportionately less to population spread than human-aided factors.","palabras_clave":"Vespula spp|avispas invasoras"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4772,"idioma_id":5,"titulo":"editorial","resumen":"El presente número 19-20 de la Revista Argentina de Ciencia Política, está dedicada a la publicación de los trabajos presentados en las Jornadas ?La democracia argentina en el último siglo?, que se realizaron los días 18 y 19 de agosto de 2016 en nuestra ciudad. Organizadas por esta revista y el Honorable Senado de la Nación, las mismas desarrollaron sus actividades, en la primer fecha, en el salón Arturo Illia del Senado y, en la segunda, en el auditorio de la Facultad de Ciencias Sociales de la Universidad de Buenos Aires. Dieron los discursos inaugurales el vicepresidente provisional de la Nación, Federico Pinedo y el Rector de la Universidad de Buenos Aires, Alberto Barbieri. El cierre estuvo a cargo de los responsables de la organización de las jornadas, el Director de la RACP, Julio Pinto, y el Senador Nacional Juan Manuel Abal Medina.","palabras_clave":"editorial"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4773,"idioma_id":4,"titulo":"Host selection by Ibalia leucospoides based on temporal variations of volatiles from the hosts? fungal symbiont","resumen":"Insect parasitoids locate hosts via reliable and predictable cues such asvolatile emissions from hosts and\/or host plants. For insects that dependon mutualistic organisms, such as many wood-boring insects, symbiont-derived semiochemicals may represent a source of such cues to beexploited by natural enemies. Ultimately, exploitation of these signalsmay increase fitness by optimizing foraging efficiency. Female parasitoidsof Ibalia leucospoides use volatiles from the fungal symbiont Amylostereumareolatum of their host Sirex noctlio to find concealed host eggs and younglarvae within the xylem. We hypothesize that the temporal pattern offungal emissions may indicate not only the presence of host larvae butalso be used as a cue that indicates host suitability and age. Such informa-tion would allow female parasitoids to discern more efficiently betweenhosts within ovipositor reach from those already buried too deep into thexylem and out of reach. In this context, we assessed the behaviour ofI. leucospoides females to volatiles of A. areolatum in a ?Y?-tube olfactometerat regular intervals over 30 days. We concurrently examined the fungalvolatiles by headspace sampling through solid-phase microextraction(SPME) followed by gas chromatography mass spectrometry (GC-MS).We observed that females were attracted to volatiles produced by two-week-old fungal cultures, a period that matches when older larvae are stillwithin ovipositor reach. Four chemical compounds were detected: etha-nol, acetone, acetaldehyde and the sesquiterpene 2,2,8-trimethyltricyclo[6.2.2.01,6]dodec-5-ene, with each compounds? relative abundancechanging over time. Results are discussed in the context of parasitoids fit-ness. Future studies involving electrophysiology, different collection tech-niques and further behavioural assays will help in identifying thecompounds that convey temporal information to female parasitoids andhave the potential for being used in integrated pest managementprogrammes.","palabras_clave":"parasitoides de sirex"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4774,"idioma_id":4,"titulo":"Multiple geophysical methods used to examine neotectonic structures in the western foothills of the Sierra de El Maitén (Argentina), North Patagonian Andes","resumen":"A high-resolution geophysical study was carried out in a region of the retroarc of the Patagonian Andes located on the western slope of the Sierra de El Maitén. This structure is characterized by an imbricated west-vergent fault system developed in the orogenic front area of the North Patagonian Andes that has uplifted. Oligocene volcanic rocks (Ventana Formation) affecting Miocene to Quaternary sediments. Even though, neotectonic fault scarps are affecting Quaternary deposits in the foothills of this range, no direct observation of slip in Quaternary strata was determined. The main objective of this study was to determine geometry of recognized neotectonic structures, characterizing them by variations in magnetic susceptibility, density and p-wave velocities. The combined application of different geophysical methods has allowed the characterization of the bedrock geometry and the determination of neotectonic displacements along faults. The potential field model and its integration with a seismic profile shows the accurate geometry of this tectonic zone, which is crucial for seismogenic hazard analysis, in area of northern Patagonia, a highly significant economic zone due to tourism with several towns (EL Maiten, Esquel, S.C. Bariloche) dispersed throughout the area of young tectonic activity.","palabras_clave":"Surface Geophysics|Neotectonic|El Maiten|Chubut"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4775,"idioma_id":4,"titulo":"Tectonic evolution of the North Patagonian Andes (41-44°S) through recognition of syntectonic strata.","resumen":"The North Patagonian fold-thrust belt (41º-44º S) is characterized by a low topography, reduced crustal thickness and a broad lateral development determined by a broken foreland system in the retroarc zone. This particular structural system has not been fully addressed in terms of the age and mechanisms that built this orogenic segment. Here, new field and seismic evidence of syntectonic strata constrain the timing of the main deformational stages, evaluating the prevailing crustal regime for the different mountain domains through time. Growth strata and progressive unconformities, controlled by extensional or compressive structures, were recognized in volcanic and sedimentary rocks from the cordilleran to the extra-Andean domain. These data were used to construct a balanced cross section, whose deep structure was investigated through a thermomechanical model that characterizes the upper plate rheology. Our results indicate two main compressive stages, interrupted by an extensional relaxation period. The first contractional stage in the mid-Cretaceous inverted Jurassic-Lower Cretaceous half graben systems, reactivating the western Cañadón Asfalto rift border ~500 km away from the trench, at a time of arc foreland expansion. For this stage, available thermochronological data reveal forearc cooling episodes, and global tectonic reconstructions indicate mid ocean ridge collisions against the western edge of an upper plate with rapid trenchward displacement. Widespread synextensional volcanism is recognized throughout the Paleogene during plate reorganization; retroarc Paleocene-Eocene flare up activity is interpreted as product of a slab rollback, and fore-to-retroarc Oligocene slab\/asthenospheric derived products as an expression of enhanced extension. The second stage of mountain growth occurred in Miocene time associated with Nazca Plate subduction, reaching nearly the same amplitude than the first compressive stage. Extensional weakening of the upper plate predating the described contractional stages appears as a necessary condition for abnormal lateral propagation of deformation.","palabras_clave":"North Patagonia|fold-thrust belt|syntectonic strata|Andean orogenesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4776,"idioma_id":5,"titulo":"MODELOS GLOBALES DE GRAVEDAD GOCE Y EGM2008: SU UTILIDAD Y COMPLEMENTARIEDAD EN LA EXPLORACIÓN GEOFÍSICA","resumen":"La reciente misión satelital GOCE cuyos objetivos principales son el estudio del campo gravitatorio terrestre y la circulación de las corrientes oceánicas, está empezando a mostrar sus resultados. El instrumental de mayor precisión diseñado específica¬mente a tal fin, como así también su órbita extremadamente baja le otorga ventajas notorias respecto de las misiones anterio¬res. Esto ha permitido generar modelos del campo de gravedad global con una precisión y homogeneidad nunca antes logra¬dos. Por otra parte, modelos como EGM2008 que integran datos de gravedad terrestre con datos satelitales, presenta la mayor resolución espacial lograda hasta el momento. El uso combinado de ambos modelos nos permite obtener información de la li¬tosfera a una escala regional aprovechando la alta precisión del primero y la alta resolución del segundo. A partir de los nuevos modelos globales de gravedad se obtienen cantidades derivadas del potencial anómalo como ser la anomalía de gravedad o el gradiente vertical de la gravedad. Este último, es de gran utilidad para delinear la localización de las estructuras geológicas con gran precisión, ya que su señal resulta centrada principalmente en la fuente. Las ventajas del uso del gradiente vertical de la gra¬vedad y de los nuevos modelos globales de gravedad con resolución y precisión cada vez mayores, se ven potenciadas gracias al desarrollo de nuevas metodologías para la corrección del efecto topográfico. La utilización de prismas esféricos de densidad constante para calcular el efecto topográfico es fundamental cuando se estudian grandes regiones, ya que una aproximación plana introduce un error considerable.","palabras_clave":"GRAVIMETRIA SATELITAL|GRADIENTE VERTICAL DE LA GRAVEDAD|EFECTO TOPOGRAFICO|TESEROIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4777,"idioma_id":5,"titulo":"Prologo","resumen":"Se trata del prólogo que me fue solicitado para abrir un monográfico sobre arte y política de la revista del GESCAL","palabras_clave":"INTEGRACIÓN LATINOAMERICANA|MEDIO AMBIENTE|GÉNERO|DERECHOS HUMANOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4778,"idioma_id":4,"titulo":"Control of infection of tomato fruits by Alternaria and mycotoxin production using plant extracts","resumen":"Tomato fruits are susceptible to infection by Alternaria species.In addition, Alternaria species may contaminate the fruits with mycotoxins.There is thus interest in control systems to minimise pathogenicity and controltoxin production. The objectives of this study were to examine the effect ofplant extracts of Eucalyptus globulus and Calendula officinalis on the growthof strains of Alternaria alternata and A. arborescens, on pathogenicity oftomato fruits and mycotoxin production. The growth bioassays showed that theethanolic and chloroformic fractions of E. globulus were the most effective inreducing growth of A. alternata (66?74 %) and A. arborescens (86?88 %), respectivelyat 2500 &#956;g\/g. The effects of plant extracts on mycotoxin biosynthesis werevariable and strain dependent. The most effective fractions in decreasing mycotoxinaccumulation were the ethanolic and chloroformic extracts of E. globulus, whichreduced tenuazonic acid by 89 %, alternariol by 75?94 % and almost completeinhibition of alternariol monomethyl ether. All the tested fractions reducedpercentage of infected tomato fruits when compared to the controls. Theethanolic and chloroformic fractions of E. globulus completely inhibited growthof A. alternata and A. arborescens on unwounded fruits and reduced theaggressiveness on wounded fruits of strains of both species significantly.","palabras_clave":"TENUAZONIC ACID|ALTERNARIOLS|ANTIFUNGAL|EUCALYPTUS GLOBULUS|CALENDULA OFFICINALIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4779,"idioma_id":4,"titulo":"Simple and novel strategies to achieve shape and size control of magnetite nanoparticles intended for biomedical applications","resumen":"AbstractMonodisperse magnetite nanoparticles (MNPs) with controlled sizes and shapes were prepared. The synthesis was carried out by traditional and inverted co-precipitation method with some modifications such as presence of visiblelight, room temperature and absence of inert atmosphere. Sodium dodecyl sulphate (SDS) was employed as stabilizer. The mentioned experimental parameters were conveniently adjusted to obtain suitable MNPs, to be efficiently employedin biomedical applications. In particular the size, shape, surface charge and magnetic properties were evaluated. MNPs were thoroughly characterized. From characterization data, it emerged that the inverted co-precipitation in presence ofvisible light rendered mainly nanorods. Modifying the SDS concentration the shape was tuned from nanorods to nanospheres and finally, at higher concentrations, nanocubes were obtained. All prepared formulations resulted hydrophilic.This property was justified in terms of the stabilization mechanism of SDS.Stability of MNPs aqueous dispersions was evaluated regarding to the variation of hydrodynamic diameter as a function of the time. In this regard, all the formulations resulted stable during, at least, 30 days. The preparednanosystems exhibited satisfactory magnetic properties with saturation magnetization slightly lower than raw magnetite.The combination of size, shape, surface charge, hidrophilicity and magnetic behaviour make the magnetic nanosystems here obtained highly suitable and promising to diagnostic and therapeutic applications.","palabras_clave":"MAGNETITE|NANOPARTICLES|CO PRECIPITATION|SODIUM DODECYL SLPHATE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4780,"idioma_id":4,"titulo":"Polymatin A from Smallanthus macroscyphus leaves: a safe and promising antidiabetic compound","resumen":"Smallanthus macroscyphus is an herb native to South America whose leaves are a source of anti-diabetic compounds, although complete information about their safe use is not available yet. This study was developed to evaluate the toxicity profile of both 10% decoction and polymatin A from S. macroscyphus leaves through in vitro cytotoxicity assays and in vivo subchronic oral toxicity. Cell viability of Hep-G2, COS1, CHO-K1 and Vero cell lines decreased in a concentration-dependent manner when cells were incubated with 0.4-200.0 µg of dry extract \/mL or 0.12-60.00 µg of polymatin A\/mL. In sub-chronic studies, decoction was orally administered to Wistar rats for 90 days at daily doses of 70, 140 and 280 mg dry extract\/kg, whereas polymatin A was administered in the same way at doses of 7, 14 and 28 mg \/kg. No toxicity signs or deaths were observed. There were no changes in the behavior, body or organ weights, hematological, biochemical or urine parameters of the rats. No histopathological lesions were observed in the examined organs. The results indicate that the 10% decoction and polymatin A from S. macroscyphus leaves may be considered as non-toxic substances at a wide range of doses, including the effective hypoglycemic dose.","palabras_clave":"Smallanthus macroscyphus leaves|polymatin A|in vitro toxicity|subchronic toxicity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4781,"idioma_id":4,"titulo":"Soybean shows an attenuated nitrogen dilution curve irrespective of maturity group and sowing date","resumen":"AbstractIt is generally accepted that the nitrogen (N) dilution curves relatingshoot nitrogen concentration (%N) and shoot biomass (W) are similar amonggenotypes from the same species. However, differences in the biomass ratio betweenmetabolic and structural tissues (Wm:Ws) would change thecurve. In soybean, maturity groups (MG) or sowing dates can lead to differencesin allocation of dry matter and nitrogen, Wm:Ws. andhence the parameters of nitrogen dilution curves.The aims of this study were:(i) to determine the growth and allocation patterns of soybean as affected bymaturity group (MG IV vs II) and sowing dateand (ii) to establish allometricrelations between growth and nitrogen, including N dilution curves. Our workinghypothesis is that the N dilution curve in soybean is similar to the critical Ndilution curve of non-legume C3 crops.Four experiments were performed at Balcarce, Argentina during threegrowing seasons with the aimto generate a wide range of growing conditions. Twosoybean cultivars were evaluated: DM2200 (MG II) and DM4970 (MG IV), sown inoptimum dates for the region (mid-November). DM2200 was also sown late (earlyJanuary). We sampled crops between W &#8776; 1 Mg ha-1and R5.Shootswereseparated in two compartments: lamina and pod (metabolic tissues; Wm)and stem and petiol (structural tissues;&nbsp;Ws).Despite the marked decrease in Wm:Ws withincreasing biomass, the N dilution curve was attenuated in soybean, with nodifferences between maturity groups II and IV, and sowing dates from Novemberto January.We propose that the increase in nitrogen concentration in laminafrom V6 to R1 in early-sown MGIV, the constant concentration in lamina and stembetween R1 and R5, and the increase in the concentration of nitrogen in podswith increasing biomass attenuated the N dilution curve. In addition, theincrease in nitrogen concentration in lamina from V6 to R1 in early-sown MGIV,couldbe the cause of the similarity in the curves across sources of variation inspite of some differences in Wm:Ws.","palabras_clave":"N dilution curve|Soybean"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4783,"idioma_id":4,"titulo":"High temperatures around flowering in maize (Zea mays L.): Effects on photosynthesis and grain yield in three genotypes","resumen":"AbstractTo aid breeding for heat-tolerant germplasm we analyzed the effects of high temperatures on the CO2 exchange rate (CER), crop growth rate (CGR), kernel number (KN), and grain yield (GY) in a 30-d period bracketing flowering. Field experiments, including three maize (Zea mays L.) hybrids with temperate (Te), tropical (Tr) and temperate × tropical (Tx) adaptation were performed in two experiments (Exp. 1 and 2). Hybrids were subjected to high temperatures induced by shelters during a 15-d period before (H1; preflowering) or after silking (H2; postflowering). Crop growth rate was measured during the 30-d period bracketing silking (CGRCP), H1 (CGRPRE), and H2 (CGRPOST). Relative to nonstressed conditions, CER was reduced by 17 and 16% in H1 and H2. Moreover, CER was associated with CGRCP (r = 0.78; p &#8804; 0.001), CGRPRE (r = 0.39; p &#8804; 0.05), CGRPOST (r = 0.51; p &#8804; 0.01), KN (Exp. 1, r = 0.53; p &#8804; 0.01; Exp. 2, r = 0.49; p &#8804; 0.01), and GY (Exp. 1, r = 0.59; p &#8804; 0.01; Exp. 2, r = 0.46; p &#8804; 0.05). As a result of heat stress, CGRCP (H1, &#8722;17%; H2, &#8722;29%), KN (H1, &#8722;7%; H2, &#8722;45%), and GY (H1, &#8722;10%; H2, &#8722;45%) were reduced relative to the control treatment. Stronger reductions for all traits in H2 relative to H1 emphasize the importance of sufficient CER during this period. The effect of high temperature on CER differed among hybrids (Tx &gt; Te = Tr) and is promising for future germplasm screening. © Crop Science Society of America.","palabras_clave":"maize|stress|temperature"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4784,"idioma_id":4,"titulo":"Cardiorenal Involvement in Metabolic Syndrome Induced by Cola Drinking in Rats: Proinflammatory Cytokines and Impaired Antioxidative Protection","resumen":"We report experimental evidence confirming renal histopathology, proinflammatory mediators, and oxidative metabolism induced by cola drinking. Male Wistar rats drank ad libitum regular cola (C, n = 12) or tap water (W, n = 12). Measures. Body weight, nutritional data, plasma glucose, cholesterol fractions, TG, urea, creatinine, coenzyme Q10, SBP, and echocardiograms (0&#8201;mo and 6&#8201;mo). At 6 months euthanasia was performed. Kidneys were processed for histopathology and immunohistochemistry (semiquantitative). Compared with W, C rats showed (I) overweight (+8%, p < 0.05), hyperglycemia (+11%, p < 0.05), hypertriglyceridemia (2-fold, p < 0.001), higher AIP (2-fold, p < 0.01), and lower Q10 level (-55%, p < 0.05); (II) increased LV diastolic diameter (+9%, p < 0.05) and volume (systolic +24%, p < 0.05), posterior wall thinning (-8%, p < 0.05), and larger cardiac output (+24%, p < 0.05); (III) glomerulosclerosis (+21%, p < 0.05), histopathology (+13%, p < 0.05), higher tubular expression of IL-6 (7-fold, p < 0.001), and TNF&#945; (4-fold, p < 0.001). (IV) Correlations were found for LV dimensions with IL-6 (74%, p < 0.001) and TNF&#945; (52%, p < 0.001) and fully abolished after TG and Q10 control. Chronic cola drinking induced cardiac remodeling associated with increase in proinflammatory cytokines and renal damage. Hypertriglyceridemia and oxidative stress were key factors. Hypertriglyceridemic lipotoxicity in the context of defective antioxidant\/anti-inflammatory protection due to low Q10 level might play a key role in cardiorenal disorder induced by chronic cola drinking in rats.","palabras_clave":"METABOLIC SYNDROME|CARDIORENAL INVOLVEMENT|COLA DRINKING|ANTIOXIDATIVE PROTECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4785,"idioma_id":4,"titulo":"exercise ameliorates endocrine pancreas damage induced by chronic cola drinking in rats","resumen":"PURPOSE:This study evaluates whether the daily practice of an exercise routine might protect from endocrine pancreas damage in cola drinking rats.METHODS:Forty-eight Wistar rats were randomly assigned to 4 groups depending on a) beverage consumption ad libitum, water (W) or cola beverage (C), and b) physical activity, sedentary (S) or treadmill running (R). Accordingly, 4 groups were studied: WS (water sedentary), WR (water runner), CS (cola sedentary) and CR (cola runner). Body weight, nutritional data, plasma levels of glucose, creatinine, total cholesterol and cholesterol fractions, and triglycerides (enzymocolorimetry), and systolic blood pressure (plethysmography) were measured. After 6 months, euthanasia was performed (overdose sodium thiopental). Pancreatic tissue was immediately excised and conventionally processed for morphometrical and immunohistochemical determinations.RESULTS:The effects of running and chronic cola drinking on pancreas morphology showed interaction (p<0.001) rather than simple summation. Cola drinking (CS vs WS) reduced median pancreatic islet area (-30%, 1.8 104 &#956;m2 vs 2.58 104 &#956;m2, p<0.0001) and median &#946;-cell mass (-43%, 3.81 mg vs 6.73 mg, p<0.0001), and increased median &#945;\/&#946; ratio (+49%, 0.64 vs 0.43, p< 0.001). In water drinking rats (WR vs WS), running reduced median &#945;-cell mass (-48%, 1.48 mg vs 2.82 mg, p<0.001) and &#945;\/&#946; ratio (-56%, 0.19 vs 0.43, p<0.0001). Differently, in cola drinking rats (CR vs CS), running partially restored median islet area (+15%, 2.06 104 &#956;m2 vs 1.79 104 &#956;m2, p<0.05), increased median &#946;-cell mass (+47%, 5.59 mg vs 3.81 mg, p <0.0001) and reduced median &#945;\/&#946; ratio (-6%, 0.60 vs 0.64, p<0.05).CONCLUSION:This study is likely the first reporting experimental evidence of the beneficial effect of exercise on pancreatic morphology in cola-drinking rats. Presently, the increase of nearly 50% in &#946; cells mass by running in cola drinking rats is by far the most relevant finding. Moderate running, advisably indicated in cola consumers and patients at risk of diabetes, finds here experimental support.","palabras_clave":"EXERCISE|ENDOCRINE PANCREAS DAMAGE|CHRONIC COLA DRINKING|RATS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4786,"idioma_id":4,"titulo":"Base free direct synthesis of alkynyl phosphonates from alkynes and H-phosphonates catalyzed by Cu2O","resumen":"A simple and mild methodology for the direct synthesis of alkynylphosphonates is presented. The reaction of a variety of terminal alkynes with dialkyl phosphites in the presence Cu2O (14 mol %) led to the formation of the corresponding alkynylphosphonates in good to excellent yields. Reactions are performed under air, in acetonitrile as solvent, and in the absence of base or ligand additives. This new methodology is compatible with the presence of a wide variety of functional groups on the starting alkynes and can be scaled up to a gram scale.","palabras_clave":"ALKYNYLPHOSPHONATES|COPPER(I) OXIDE|C-H ACTIVATION |BASE FREE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4787,"idioma_id":4,"titulo":"Biological activities of Zn(II)-S-methyl-cysteine complex as antiradical, inhibitor of  acid phosphatase enzyme and in vivo antidepressant effects","resumen":"The antidepressant-like effect of simple Zn(II) salts hasbeen proved in several animal models of depression. In this study, acoordination metal complex of Zn(II) having a sulfur containing ligand istested as antidepressant for the first time. Forcedswimming test method on male Wistar rats shows a decrease in the immobility and anincrease in the swimming behavior after treatment with [Zn(S-Met)2](S-Met=S-methyl-L-cysteine) being more effective andremarkable than ZnCl2. The thiobarbituric acid and thepyranine consumption (hydroxyland peroxyl radicals, respectively) methodswere applied to evaluate the antioxidant activity of S-Met and [Zn(S-Met)2] showingevidence of attenuation ofhydroxyl but not peroxyl radicals activities. UV-vis studies on the inhibitionof acid phosphatase enzyme (AcP) demonstrated that S-methyl-L-cysteine did notproduce any effect but, in contrast, [Zn(S-Met)2] complex behaved as a moderate agent. Finally, bioavailability studies wereperformed by fluorescence spectroscopy denoting the ability of the albumin totransport the complex. ","palabras_clave":"ANTIDEPRESSANT EFFECT|S-METHYL-CYSTEINE|PHOSPHATASE INHIBITORY EFFECTS|ANTIOXIDANT ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4788,"idioma_id":5,"titulo":"Redescubrimiento  de  dos  especies  de  Toxotrypana ( Diptera:  Tephritidade )  en Argentina","resumen":"Redescubrimiento de dos especies de Toxotrypana (Diptera: Tephritidade) en Argentina","palabras_clave":"Mosca de los frutos|Trampas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4789,"idioma_id":4,"titulo":"B. abortus invasion of osteocyte modulates connexin 43 and integrins expression and induces osteoclastogenesis via RANKL and TNF-&#945; secretion","resumen":"Osteoarticular brucellosis is the most common localization of human active disease. Osteocytes are the most abundant cells ofbone. They secrete factors that regulate the differentiation of both osteoblasts and osteoclasts during bone remodeling. The aimof this study is to determine if Brucella abortus infection modifies osteocyte function. Our results indicate that B. abortus infectioninduced matrix metalloproteinase 2 (MMP-2), receptor activator for NF-B ligand (RANKL), proinflammatory cytokines,and keratinocyte chemoattractant (KC) secretion by osteocytes. In addition, supernatants from B. abortus-infected osteocytesinduced bone marrow-derived monocytes (BMM) to undergo osteoclastogenesis. Using neutralizing antibodies against tumornecrosis factor alpha (TNF-) or osteoprotegerin (OPG), RANKL?s decoy receptor, we determined that TNF- and RANKL areinvolved in osteoclastogenesis induced by supernatants from B. abortus-infected osteocytes. Connexin 43 (Cx43) and the integrinsE11\/gp38, integrin-, integrin-, and CD44 are involved in cell-cell interactions necessary for osteocyte survival. B. abortusinfection inhibited the expression of Cx43 but did not modify the expression of integrins. Yet the expression of both Cx43 andintegrins was inhibited by supernatants from B. abortus-infected macrophages. B. abortus infection was not capable of inducingosteocyte apoptosis. However, supernatants from B. abortus-infected macrophages induced osteocyte apoptosis in a dose-dependentmanner. Taken together, our results indicate that B. abortus infection could alter osteocyte function, contributing tobone damage.","palabras_clave":"Brucella"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4790,"idioma_id":4,"titulo":"SPINICAUDATANS FROM THE UPPER JURASSIC OF ARGENTINA AND THEIR PALEOENVIRONMENTS","resumen":"Spinicaudatans are a diverse and geographically widespread group of small branchiopod crustaceans with an excellent fossil record. They inhabit ephemeral freshwater habitats with warm conditions and pH levels from neutral to alkaline. Many studies have been performed on the systematics of this group, also known as ?conchostracans?, although the paleoecology of these clam shrimp has not been widely analyzed. This paper examines some aspects of the relationship between spinicaudatans and their environments from the Upper Jurassic (Ca?nad´on Asfalto Formation) of Argentina. We used sedimentological analysis to infer paleoenvironmental factors. Spinicaudatan features were also measured, including carapace size, growth band width, and growth lines. The results of this study reveal that the observed spinicaudatan species (Congestheriella rauhuti, Wolfestheria smekali, and Wolfestheria sp.) would have lived in carbonate lacustrine systems characterized by benthic microbial communities dominated by cyanobacteria. However, environmental factors such as oxygenation, pyroclastic deposits, and associated fauna (fish, ostracods, bivalves, and charophytes) were different at each locality. The spinicaudatan carapaces also show varying morphological features such as small or large carapace size, narrow or wide growth bands and low or high density of growth lines. These differences are related to abiotic (volcanic-oxygen) and biotic (fish and microbial) factors regarded as important agents for the development of the spinicaudatan populations.","palabras_clave":"CONCHOSTRACANS|PALEOECOLOGY|CAÑADON ASFALTO FORMATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4791,"idioma_id":4,"titulo":"Glial Cell-Elicited Activation of Brain Microvasculature in Response to Brucella abortus Infection Requires ASC Inflammasome-Dependent IL-1&#946; Production","resumen":"Blood-brain barrier activation and\/or dysfunction are a common feature of human neurobrucellosis, but the underlying pathogenic mechanisms are largely unknown. In this article, we describe an immune mechanism for inflammatory activation of human brain microvascular endothelial cells (HBMEC) in response to infection with Brucella abortus. Infection of HBMEC with B. abortus induced the secretion of IL-6, IL-8, and MCP-1, and the upregulation of CD54 (ICAM-1), consistent with a state of activation. Culture supernatants (CS) from glial cells (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a greater extent. Although B. abortus-infected glial cells secreted IL-1b and TNF-&#945;, activation of HBMEC was dependent on IL-1b because CS from B. abortus-infected astrocytes and microglia deficient in caspase-1 and apoptosis-associated speck-like protein containing a CARD failed to induce HBMEC activation. Consistently, treatment of CS with neutralizing anti-IL-1b inhibited HBMEC activation. Both absent in melanoma 2 and Nod-like receptor containing a pyrin domain 3 are partially required for caspase-1 activation and IL-1b secretion, suggesting that multiple apoptosis-associated speck-like protein containing CARD-dependent inflammasomes contribute to IL-1b-induced activation of the brain microvasculature. Inflammasomemediated IL-1b secretion in glial cells depends on TLR2 and MyD88 adapter-like\/TIRAP. Finally, neutrophil and monocyte migration across HBMEC monolayers was increased by CS from Brucella-infected glial cells in an IL-1b-dependent fashion, and the infiltration of neutrophils into the brain parenchyma upon intracranial injection of B. abortus was diminished in the absence of Nod-like receptor containing a pyrin domain 3 and absent in melanoma 2. Our results indicate that innate immunity of the CNS set in motion by B. abortus contributes to the activation of the blood-brain barrier in neurobrucellosis and IL-1b mediates this phenomenon.","palabras_clave":"BRUCELLA ABORTUS|NEUROBRUCELLOSIS|INFLAMMASOME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4792,"idioma_id":4,"titulo":"Data on polyphenols and biological activity analyses of an Andean tomato collection and their relationships with tomato traits and geographical origin","resumen":"Data provide information about a tomato collection composed of accessions from the Andean Valley,commercial accessions and wild Species. Antioxidant metabolites were measured inmature fruits of this collection,and their biological activities were assessed by both in vitro and invivo methods.In this work,the parameters used to identify and quantify polyphenols compounds in tomato fruit by liquid chromatography coupled to diode array detector and quadrupole time of flight mass spectrometer are described. Moreover,data supporting a procedure to characterize the properties of tomato fruits to revert death by thermal stress in Caenorhabditis elegans are explained in detail. Lastly,principal component analysis and hierarchical cluster analysis of metabolites composition, antioxidant activities(invivo and invitro),tomato traits and geographical origin of the tomatoes collection.","palabras_clave":"tomato|polyphenols|antioxidant|biological activity|Caenorhabditis elegans"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4793,"idioma_id":4,"titulo":"Hydrophilic antioxidants from Andean Tomato Landraces assessed by their bioactivities in vitro and in vivo","resumen":"Potential nutraceutical properties of hydrophilic antioxidants in fruits of tomato landraces collected in Andean valleys were characterised. Antioxidant metabolites were measured by HPLC?DAD?MS\/MS in mature fruits and their biological activities were assessed by in vitro and in vivo methods. In vitro antioxidant capacities were established by TEAC and FRAP methods. For in vivo biological activities we used a procedure based on Caenorhabditis elegans subjected to thermal stress. In addition, Saccharomyces cerevisiae was also used as a rapid screening system to evaluate tomato antioxidant capacity. All tomato accessions displayed significant differences regarding metabolic composition, biological activity and antioxidant capacity. Metabolite composition was associated with geographical origin and fruit size. Antioxidant activities showed significant association with phenolic compounds, such as caffeoylquinic acids, ferulic acid-O-hexosides and rutin. Combination of in vitro and in vivo methods applied here allowed evaluation of the variability in nutraceutical properties of tomato landraces, which could be applied to other fruits or food products.","palabras_clave":"Tomato landraces|Solanum lycopersicum|Phenolic profile|Antioxidant capacity|Caenorhabditis elegans|Saccharomyces cerevisiae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4797,"idioma_id":4,"titulo":"Assessment of the Abundance and Diversity of Calliphoridae and Sarcophagidae (Diptera) in Sites With Different Degrees of Human Impact in the Ibera Wetlands  (Argentina)","resumen":"The aim of the present study was to explore the diversity and describe the spatial distribution of Calliphoridae and Sarcophagidae along an urbanization gradient in the Iberá Wetlands. The flies were collected at 18 sampling points, three per site. In total 3,318 flies were collected (3,077 blow flies and 241 flesh flies), distributed in 13 genera and 33 species. Calliphoridae was the most abundant, comprised 92.74% of all adult flies. Chrysomya albiceps (Wiedeman), Cochliomyia macellaria (F.), Ch. megacephala (F.) and Ch. putoria (Wiedemann 1830) were the most abundant species representing 82.21% of the total sample. The genus Oxysarcodexia Townsend was the most diverse with 10 species represented. The abundance of exotic species represents 62.85% of the total sample, showing a reduction towards less disturbed sites. The results of this study indicated that sites with intermediate impact had higher diversity than those where the disturbances are high or absent. Our findings provide the first assessment of Calliphoridae and Sarcophagidae diversity in the area of the Iberá Wetlands.","palabras_clave":"ANTHROPOGENIC IMPACTS|BIODIVERSITY|CALYPTRATAE|HUMAN IMPACT |NEOTROPICAL REGION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4798,"idioma_id":4,"titulo":"TiO2 Modified with Polyoxotungstates should Induce visible-light Absorption and High Photocatalytic Activity Through the formation of surface complexes","resumen":"TiO2 nanospherical particlesprepared by the sol-gel method were modified with tungstophosphoric (TPA) andtungstosilicic acid (TSA) at 30% (w\/w) and then annealed at 500 ºC for 2 h.Materials were characterized by multitechniques, such as scanning electronmicroscopy with energy dispersive X-ray spectroscopy (SEM-EDX), FT-Ramanspectroscopy, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD),diffuse reflectance spectroscopy (DRS), 31P, 1H and 29Simagic angle spinning nuclear magnetic resonance (MAS-NMR). Acidity andisoelectric point measurements were also performed. DRS results showed evidenceof visible light absorption of TiO2-TPA and TiO2-TSA. MAS-NMR,XPS and FT-Raman characterization revealed evidence of a strong interactionbetween the Keggin anion of TPA or TSA and TiO2 surfaces, possiblypromoted by the formation of surface heteropolyacid-TiO2 complexes; thelatter should be responsible of its visible light absorption. Photocatalyticactivity was evaluated in the degradation of malachite green (MG), a cationictriphenylmethane dye, and 4-chlorophenol (4-CP) aqueous solutions under UV-Aand visible light irradiations. Under UV-A light, TPA-TiO2 andTSA-TiO2 materials showed high photocatalytic activity towards MGdegradation, even higher than that of TiO2 P-25. When 4-CP wasdegraded, TiO2 P-25 exhibited higher photocatalytic activity. Undervisible light irradiation and using the modified photocatalysts, malachitegreen oxidation was achieved, while 4-chlorophenol reached almost 20% ofdegradation. Mechanisms of photocatalytic oxidation either under UV-A orvisible irradiation are discussed.","palabras_clave":"Tungstophosphoric acid|tungstosilicic acid|TiO2 with visible light absorption|heterogeneous photocatalysis|heteropolyacids"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4799,"idioma_id":4,"titulo":"Complex relationship between sex hormones, insulin resistance and leptin in men with and without prostatic disease","resumen":"Objectives: to assess sex hormones, leptin and insulin-resistance in men with Prostate cancer (PCa) and Benign Prostatic Hyperplasia (BPH) and to study associations between androgens and histologic score of prostate tissue in PCa. Subjects and methods: Two hundred ten men older than 45 years selected from 2906 participants of a population screening for PCa were studied: 70 with PCa, 70 with BPH and 70 controls (CG), matched by body mass index and age. Insulin, IGF-1, PSA, leptin, total, free and bioavaliable-testosterone (bT) and estradiol were measured. Each group was subdivided into two subgroups considering the presence of metabolic syndrome (MS); androgens and leptin levels were analyzed in the subgroups. Results: Prostate cancer and BPH patients presented higher total, free and bT levels than CG. IGF-1, insulin and HOMA index were higher in BPH than in the other two groups. PCa presented higher leptin [median (range) 6.5 (1.3?28.0) versus 4.8 (1.1?12.3) ng\/ml; p<0.01] and estradiol [median (range) 37.0 (20?90) versus 29.0 (20?118) pg\/ml; p=0.025] levels than CG. After dividing men considering the presence of MS, leptin was higher and total testosterone was lower in MS patients in all the groups. Conclusions: it was observed a coexistence of an altered hormone profile with increased sex hormones and leptin in PCa patients, in accordance with the new perspective of PCa pathogenesis.","palabras_clave":"SEX HORMONES|LEPTIN| PROSTATIC DISEASES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4800,"idioma_id":5,"titulo":"¿Qué atributos busca el consumidor en prendas de vestir? Un análisis aplicado al mercado de la indumentaria en Argentina","resumen":"El principal objetivo del presente trabajo es analizar las decisiones de compra de los consumidores de productos de la industria textil-confecciones con marca y diseño. Para ello, mediante un abordaje cuantitativo, se encuestó, dentro de locales comerciales de indumentaria, tanto a compradores como a no compradores. El estudio se realizó en la ciudad de Mar del Plata (Argentina). Los principales resultados indican que los factores que inciden en la decisión efectiva de compra corresponden a atributos del producto, tales como calidad y diseño, a los esfuerzos de la firma en la fidelización de los clientes y a características propias del consumidor.","palabras_clave":"DECISIONES DE COMPRA|DISEÑO Y MARCA|SECTOR TEXTIL-CONFECCIONES|REGRESIÓN LOGÍSTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4801,"idioma_id":4,"titulo":"Inferring field performance from drought experiments can be misleading: The case of symbiosis between grasses and Epichloë fungal endophytes","resumen":"No tiene.&nbsp;","palabras_clave":"Plant-fungus interaction|Mutualism|Aridity|Productivity|Mean annual precipitation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4802,"idioma_id":4,"titulo":"Estimating overnight weight loss of corralled yearling steers in semiarid rangeland","resumen":"? A common practice for assessing livestock weight gains from grazing animals on rangelands is to confine animals overnight without feed or water to reduce variation in weight loss and percent shrink.? Advances in remote sensing of vegetation, such as the Normalized Difference Vegetation Index (NDVI) provide opportunities to estimate greenness (an indicator of both the quality and quantity of the plant community) that could be used with air temperature and relative humidity as predictors of percent shrink in grazing animals.? We determined percent shrink losses from crossbred yearling steers at each of four weigh dates for four consecutive years.? Percent overnight shrink by yearling steers grazing semiarid rangeland was influenced positively by air temperature and NDVI values, but not relative humidity.? The prediction equation we developed can provide temporal weight gain data within a grazing season without the logistical difficulties in gathering and holding animals, as well as eliminate associated animal stress from shrinking and regaining gut fill multiple times.","palabras_clave":"air temperature|overnight shrink|relative humidity|remote sensing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4803,"idioma_id":4,"titulo":"Abnormal proplatelet formation and emperipolesis in cultured human megakaryocytes from gray platelet syndrome patients.","resumen":"The Gray Platelet Syndrome (GPS) is a rare inherited bleeding disorder characterized by deficiency ofplatelet &#945;-granules, macrothrombocytopenia and marrow fibrosis. The autosomal recessive form ofGPS is linked to loss of function mutations in NBEAL2, which is predicted to regulate granule traffickingin megakaryocytes, the platelet progenitors. We report the first analysis of cultured megakaryocytesfrom GPS patients with NBEAL2 mutations. Megakaryocytes cultured from peripheral blood or bonemarrow hematopoietic progenitor cells from four patients were used to investigate megakaryopoiesis,megakaryocyte morphology and platelet formation. In vitro differentiation of megakaryocytes wasnormal, whereas we observed deficiency of megakaryocyte &#945;-granule proteins and emperipolesis.Importantly, we first demonstrated that platelet formation by GPS megakaryocytes was severelyaffected, a defect which might be the major cause of thrombocytopenia in patients. These resultsdemonstrate that cultured megakaryocytes from GPS patients provide a valuable model to understandthe pathogenesis of GPS in humans.","palabras_clave":"GRAY PLATELET|NBEAL2|THROMBOPOIESIS|MEGAKARYOCYTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4804,"idioma_id":4,"titulo":"First record of Aphyocharax anisitsi Eigenmann & Kennedy, 1903 in the upper Bermejo River basin, northwestern Argentina","resumen":"AbstractAphyocharax anisitsi is recorded for the first time from the upper Bermejo River, Argentina, in the provinces of Salta and Jujuy. This represents a range extension of the known distribution of approximately 600 km. Also, collection specimens of A. dentatus from this area are cited for first time.ResumenAphyocharax anisitsi es registrado por primera vez en la Cuenca alta del Río Bermejo, Argentina, en las provincias de Salta y Jujuy. Esto representa una extensión de la distribución conocida de aproximadamente 600 km. Además, se cita material de colección de A. dentatus para el área por primera vez.","palabras_clave":"Biogeography|upper Bermejo river Basin|Characidae|Ostariophysi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4805,"idioma_id":4,"titulo":"Modeling human reliability: the underlying cognitive abilities","resumen":"[Journal Indexado por Scimago, Directory of Open Access Journals (DOAJ), Cite Factor, Scopus, entre otros] The innovative inclusion of the Cattell-HornCarroll theory on cognitive abilities and Fleishman?s premises on social and interpersonal skills simultaneously addresses several problems of existing human reliability models. First, it provides a theoretical basis for causal cognitive models of human error with a strong experimental support specifying their interrelationships and dependencies. Derived from psychometric studies, this quantitative theory facilitates the quantification of the parameters required for a quantitative analysis of human reliability while also reducing the subjectivity in the analysis. Herein, the inclusion of cognitive, social and interpersonal skills puts focus on interpersonal differences and their impact on human performance. In other words, an analysis of operators, a new dimension of analysis in human reliability models, is hereby introduced for the first time.","palabras_clave":"HUMAN RELIABILITY ANALYSIS|COGNITIVE ABILITIES|HUMAN PERFORMANCE|HUMAN ERROR|OPERATOR ANALYSIS|JOB ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4806,"idioma_id":4,"titulo":"In Vivo and In Vitro Arsenic Exposition Induces Oxidative Stress in Anterior Pituitary Gland","resumen":"Inorganic arsenic (iAs) is at the top of toxic metalloids. Inorganic arsenic-contaminated water consumption is one of the greatest environmental health threats worldwide. Human iAs exposure has been associated with cancers of several organs, neurological disorders, and reproductive problems. Nevertheless, there are no reports describing how iAs affects the anterior pituitary gland. The aim of this study was to investigate the mechanisms involved in iAs-mediated anterior pituitary toxicity both in vivo and in vitro. We showed that iAs administration (from 5 to 100 ppm) to male rats through drinking water increased messenger RNA expression of several oxidative stress-responsive genes in the anterior pituitary gland. Serum prolactin levels diminished, whereasluteinizing hormone (LH) levels were only affected at the higher dose tested. In anterior pituitary cells in culture, 25 micromol\/L iAssignificantly decreased prolactin release in a time-dependent fashion, whereas LH levels remained unaltered. Cell viability was significantly reduced mainly by apoptosis evidenced by morphological and phosphatidylserine externalization studies. This process is characterized by early depolarization of mitochondrial membrane potential and increased levels of reactive oxygen species. Expression of some key oxidative stress-responsive genes, such as heme oxygenase-1 and metallothionein-1, was also stimulated by iAs exposure. The antioxidant N-acetyl cysteine prevented iAs-induced effects on the expression of oxidative stress markers, prolactin release, and apoptosis. In summary, the present work demonstrates for the first time that iAs reduces prolactin releaseboth in vivo and in vitro and induces apoptosis in anterior pituitary cells, possibly resulting from imbalanced cellular redox status.","palabras_clave":"ARSENIC|OXIDATIVE STRESS|APOPTOSIS|ANTERIOR PITUITARY CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4807,"idioma_id":4,"titulo":"Hydrography of the inner continental shelf along the southwest Buenos Aires Province, Argentina:  Influence of an estuarine plume on coastal waters","resumen":"Estuarine plumes have a significant influence on the characteristics and biology of the adjacent inner shelf. In the sector occupied by the plume several transformations occur to the sediment and nutrients exported from the continent to the oceans. The coastal zone of Pehuén Co (PC) and Monte Hermoso (MH) is located in a highly complex oceanographic and ecological regional system, which creates the basis of one of the most valuable Argentinean habitats for fishing of commercial species.  In this work the physical oceanographic characteristics of the inner shelf of the southwest Buenos Aires Province and the influence of the Bahia Blanca estuarine (BBE) waters are introduced. The in situ data was obtained from oceanographic cruises were vertical profiles of water temperature, salinity and turbidity were made. The spatial variability of the parameters was studied with a multivariate statistical analysis. Temperature and salinity presented high seasonal variability (6.1?22.9 °C; 33.5-36). Maximum turbidity occurs throughout summer (64 NTU) and minimum during spring (24 NTU). The study area was characterized by a significant spatial variability, especially in terms of salinity and turbidity, meanwhile water temperature showed a more homogeneous pattern. Salinity and turbidity values decreased from PC (closer to BBE) to MH (further to BBE) stations, with the highest values located near the mouth of the BBE. PC and MH (in a lesser extent) inner shelves are transitional environments between the estuary and the open beaches of Buenos Aires Province, creating a habitat where benthic and zooplankton estuarine and marine species are found.","palabras_clave":"COASTAL WATERS|TEMPERATURE|SALINITY|TURBIDITY|BAHÍA BLANCA ESTUARY|ESTUARINE PLUME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4808,"idioma_id":4,"titulo":"Congruence of phenotypic and genetic variation at the subspecific level in a Neotropical passerine","resumen":"The increasing availability of molecular phylogenies has highlighted the issue that genotypic and phenotypic patterns of variation are not always congruent, particularly below the species level. This has led to an ongoing discussion on the validity of the subspecies category and on the use of molecular data to help revise traditional classifications based on phenotypic data. We compared patterns of spatial diversification in genotype and phenotype in the Blue-black Grosbeak Cyanocompsa cyanoides, a Neotropical songbird with four recognised subspecies. Variation in phenotype and genotype are partially congruent among the four subspecies. The more genetically divergent subspecies C. c. rothschildii is strongly differentiated from the other subspecies in meristic characters, plumage colo uration and song . W e suggest that this taxon be accorded full species status as C. rothschildii. Regarding the remaining diversity within C. cyanoides, both phenotypic and genetic markers suggests that it could be divided into two subgroups, but the boundaries of genetic lineages do not coincide with those of subspecies defined using phenotypic data. Lack of complete congruence between phenotypic and molecular markers may be expected, since they are subject to different evolutionary processes. The discordance could also be due to a methodological problem, as subspecies ? geographic boundaries were defined on the basis of phenotypic descriptions that were not systematically evaluated. We consider the subspecies to be an informative taxonomic entity , but note that many current subspecific designations for diverse species need extensive reassessment.","palabras_clave":"SUBESPECIES|PLUMAGE COLORATION|SONG|BODY SIZE|MITOCHONDRIAL DNA MARKERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4809,"idioma_id":4,"titulo":"Calibrating the molecular clock beyond cytochrome b: assessing the evolutionary rate of COI in birds","resumen":"Estimating the age of species or their component lineages based onsequence data is crucial for many studies in avian evolutionary biology.Although calibrations of the molecular clock in birds have been performedalmost exclusively using cytochrome b (cyt b), they are commonlyextrapolated to other mitochondrial genes. The existence of a large,standardized cytochrome c oxidase subunit I (COI) library generated as aresult of the DNA barcoding initiative provides the opportunity to obtain acalibration for this mitochondrial gene in birds. In this study we comparethe evolutionary rate of COI relative to cyt b across ten different avianorders. We obtained divergence estimates for both genes from nearly 300phylogenetically independent pairs of species through the analysis ofalmost 5000 public sequences. For each pair of species we calculated thedifference in divergence between COI and cyt b. Our results indicate thatCOI evolves on average 14% slower than cyt b, but also revealconsiderable variation both among and within avian orders, precluding theuse of this value as a standard adjustment for the COI molecular clock forbirds. Our findings suggest that this variation is partially explained by aclear negative relationship between the difference in divergence in thesegenes and the age of species. Distances for cyt b are higher than those forCOI for closely related species, but the values become similar as thedivergence between the species increases. This appears to be the result ofa stronger pattern of negative time-dependency in the rate of cyt b than inthat of COI, a difference that could be related to lower functionalconstraints on a small number of sites in cyt b that allow it to initiallyaccumulate mutations more rapidly than COI.","palabras_clave":"molecular clock|cytochrome c oxidase subunit I (COI)|cytochrome b (cyt b)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4810,"idioma_id":4,"titulo":"Survey and  first  molecular characterization of Echinococcus granulosus sensu   stricto  (G1)   in  Pampas fox (Lycalopex gymnocercus) in Buenos Aires province, Argentina","resumen":"Echinococcosis is a zoonosis caused by tapeworms of the genus Echinococcus. Echinococcus granulosus sensu lato (s. l.) has a world-wide distribution and its transmission is primarily maintained in a synanthropic cycle with dogs as definitive hosts and livestock species as intermediate hosts. However, many wild canids also function as definitive hosts for E. granulosus s. l. Echinococcosis in humans is mainly caused by E. granulosus sensu stricto (s. s.) G1 genotype. In the present work, we expanded the epidemiological study on echinococcosis reported cases in Pampas fox (Lycalopex gymnocercus) to provide a prevalence estimate for rural areas of southern Buenos Aires province, Argentina. Ninety-five whole intestines were analyzed using the sedimentation and counting technique with a result of 83 foxes (87.37%) harboring at least one helminth species. Echinococcus granulosus s. l. adults were found in one Pampas fox (1.05%). These adult helminthes were E. granulosus s. s. (G1) according to the genotyping analysis of a 450-bp region of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene.","palabras_clave":"ECHINOCOCCOSIS|ZOONOSES|COX1|EPIDEMIOLOGY|WILDLIFE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4811,"idioma_id":4,"titulo":"Ancylostoma (Ancylostoma) buckleyi (Nematoda: Ancylostomatidae): new wild host and distribution expansion","resumen":"Here we report the occurrence of Ancylostoma (Ancylostoma) buckleyi Le Roux and Biocca, 1957 (Nematoda:Ancylostomatidae) in the small intestine of Pampas foxes (Lycalopex gymnocercus) (Mammalia: Canidae). This fox is themost abundant native carnivore in southern South America, where it inhabits grasslands, open woodlands and areashighly modified by extensive ranching and agricultural activities. Material from 80 foxes in rural areas of southern BuenosAires province, Argentina was examined. The intestinal tracts were carefully removed from each carcass and subsequentlyisolated by ligatures (pylorus and rectum). Examination of the intestinal content was performed using the sedimentationand counting technique. Four foxes (5%) were found to be parasitized with adult specimens of A. buckleyi. This is thefirst report of Ancylostoma (A.) buckleyi in Argentina and adds L. gymnocercus as new host of this nematode species.","palabras_clave":"LYCALOPEX GYMNOCERCUS|PAMPAS FOX|ANCYLOSTOMA BUCLEYI|ARGENTINA|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4812,"idioma_id":4,"titulo":"Nanofibrillated cellulose (CNF) from eucalyptus sawdust as a dry strength agent of unrefined eucalyptus handsheets","resumen":"Nanofibrillated cellulose has been obtained from the cellulosic fraction of eucalyptus sawdust. The fractionation process involved the partial removal of hemicelluloses and lignin. CNF was obtained using TEMPO oxidation with NaOCl in basic medium followed by mechanical homogenization. The obtained CNF was subsequently used as a dry strength agent on unbleached unrefined eucalyptus pulp. The addition of 3, 6 and 9 wt. % of CNF increased lineally the tensile index of handsheets to about 55 N?m?g-1 at 35 °SR, compatible with papermachine runnability. The other mechanical properties also increased substantially, and porosity decreased moderately. The estimated specific surface and average diameter of these CNF were 60 m2 g-1, and of 41.0 nm, respectively. The addition of 9 wt. % of CNF produced an increase in mechanical strength, equivalent to that produced by PFI refining at 1600 revolutions.","palabras_clave":"BIOREFINERY|EUCALYPTUS SAWDUST|NANOFIBRILLATED CELLULOSE|PAPERMAKING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4813,"idioma_id":4,"titulo":"Small- sized biorefineries as strategy to add value to sugarcane bagasse","resumen":"Small-sizedbiorefineries are not capital intensive and have lower transportation cost,lesser movements of liquid and solid streams, and lower heat transfer problemsthan high-sized ones. In this work, different scenarios were investigated inorder to determine technical and economic viability of different processes toproduce valuable products from sugarcane bagasse by small-sized biorefineries.Different alternatives were evaluated, as furfural and xylitol production fromhemicelluloses, as well as energy generation and medium-density fibreboard(MDF) production from the residual solid after hemicelluloses hydrolysis.Experimental data obtained by the authors in previous works were used forpretreatment steps, and updated literature data was used for the otherprocesses. A simplified kinetic model was developed for the extraction ofxylose, and unit operations processes were selected and simulated. The mostprofitable option was determined for hemicelluloses use. In addition, costs andbenefits that could be obtained exploiting the residual solid feedstock wereestimated. The economic evaluation shows that recovery periods are extensive inboth scenarios. However, optimization of different steps as liquid to solidratio of pretreatment, evaporation, or fermentation, could represent attractiveand innovative alternatives in order to reduce the recovery periods of capitalcosts.","palabras_clave":"BIOREFINERY|SUGARCANE|BAGASSE|XYLOSE|FURFURAL|XYLITOL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4814,"idioma_id":4,"titulo":"Beneficial effect of moderate exercise in kidney of rat after chronic consumption of cola drinks","resumen":"AimThe purpose of this study was to investigate the effect of moderate intensity exercise on kidney in an animal model of high consumption of cola soft drinks.MethodsForty-eight Wistar Kyoto rats (age: 16 weeks; weight: 350 ? 400 g) were assigned to the following groups: WR (water runners) drank water and submitted to aerobic exercise; CR (cola runners) drank cola and submitted to aerobic exercise; WS (water sedentary) and CS (cola sedentary), not exercised groups. The aerobic exercise was performed for 5 days per week throughout the study (24 weeks) and the exercise intensity gradually increased during the first 8 weeks until it reached 20 meters \/ minute for 30 minutes. Body weight, lipid profile, glycemia, plasma creatinine levels, atherogenic index of plasma (AIP) and systolic blood pressure (SBP) were determined. After 6 month rats were sacrificed. A kidney histopathological score was obtained using a semiquantitative scale. Glomerular size and glomerulosclerosis were estimated by point-counting. The oxidative stress and pro-inflammatory status were explored by immunohistochemistry. A one way analysis of variance (ANOVA) with Tukey-Kramer post-hoc test or the Kruskal-Wallis test with Dunn?s post-hoc test were employed for statistics. A value of p < 0.05 was considered significant.ResultsAt 6 month, an increased consumption of cola soft drink were showed in CS and CR compared with water consumers (p<0.0001). While chronic cola consumption was associated with increased plasma triglycerides, AIP, heart rate, histopathological score, glomerulosclerosis, oxidative stress and pro-inflammatory status, moderate exercise prevented these findings. No difference was observed in the body weight, SBP, glycemia, cholesterol and plasma creatinine levels across experimental groups.ConclusionsThis study warns about the consequences of chronic consumption of cola drinks on lipid metabolism, especially regarding renal health. Additionally, these findings emphasize the protective role of exercise training on renal damage.","palabras_clave":"cola drinks|hypertriglyceridemia|renal pathology|redoxin system|moderate exercise|metabolic syndrome"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4815,"idioma_id":4,"titulo":"Mineral and essential element measurements in dolphin bones using two analytical approaches","resumen":"We explored the potential of using energy-dispersive X-ray analysis (EDX), a non-destructive technique, to assess elemental contents in dolphin bones. Specimens were deposited in museum collections, and prepared by different methodologies. Fifty eight Commerson?s dolphins (Cephalorhynchus c. commersonii) chevron bones and 24 Franciscana dolphins (Pontoporia blainvillei) were analysed. The EDX allowed us to detect the following elements: Ca, P, Na, Mg, Fe, K, Zn, S, Cl and Al; and quantify their proportion (weight percent of element). Principal components analysis differentiates two groups according to the cleaning procedures applied, supporting that cleaning methods could influence the chemical integrity of bone. No significant age-dependent increase was found for elements analysed in species, and no significant differences were found between sex and physical maturity stages. Alternative assessment was made through atomic absorption spectrophotometry, providing quantitative information on the principal elements in bones (Ca, P, Mg, Na, Fe and Zn) and allowing comparisons with other studies. A standard protocol for bone cleaning and conditioning is needed to exclude any effect on the mineral integrity of calcified tissue. This would enable future comparative studies on the bone mineral matrix over time housed in natural history museums or other scientific collections.KEYWORDS: Elements, calcified tissue, skeleton, odontocetes, southwestern south Atlantic Ocean","palabras_clave":"ELEMENTS|CALCIFIED TISSUE|SKELETON|ODONTOCETES| SOUTHWESTERN SOUTH ATLANTIC OCEAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4816,"idioma_id":5,"titulo":"'CYSTIC FIBROSIS CLOUD DATABASE': UN SISTEMA INFORMÁTICO PARA EL ALMACENAMIENTO Y MANEJO DE DATOS CLÍNICOS Y MICROBIOLÓGICOS DEL PACIENTE CON FIBROSIS QUÍSTICA","resumen":"The epidemiological and clinical management of cystic fibrosis (CF) patients suffering from acute pulmonary exacerbations or chronic lung infections demands continuous up dating of medical and microbiological processes associated with the constant evolution of pathogens during host colonization. In order to monitor the dynamics of these processes, it is essential to have expert systems capable of storing and subsequently extracting the information generated from different studies of the patients and microorganisms isolated from them. In this work we have designed and developed an on-line database based on an information system that allows to store, manage and visualize data from clinical studies and microbiological analysis of bacteria obtained from the respiratory tract of patients suffering from cysticfibrosis. The information system, named Cystic Fibrosis Cloud database is available on the http:\/\/servoy.infocomsa.com\/cfc database site and is composed of a main database and a web-based interface, which uses Servoy?s product architecture based on Java technology. Although the CFC database system can be implemented as a local program for private use in CF centers, it can also be used, updated and shared by different users who can access the stored information in a systematic, practical and safe manner. The implementation of the CFC database could have a significant impact on the monitoring of respiratory infections, the prevention of exacerbations, the detection of emerging organisms, and the adequacy of control strategies forlung infections in CF patients.","palabras_clave":"FIBROSIS QUÍSTICA|BASE DE DATOS|VISUALIZACIÓN DE DATOS|MONITOREO DE PACIENTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4817,"idioma_id":4,"titulo":"Beak deviations in the skull of Franciscana dolphins Pontoporia blainvillei from Argentina","resumen":"The Franciscana dolphin Pontoporia blainvillei is characterized by a long rostrum,a feature that is shared with the families formerly classified as river dolphins (Pontoporiidae,Platanistidae, Iniidae, Lipotidae). Although there are occasional reports on the existence of beakdeformations, very little published information exists describing this process. The object of thepresent study was to describe and quantify the beak anomalies of Franciscana dolphins from thecoastal waters of Argentina. Of 239 skulls analyzed 12% showed beak deviations (BD), affectingthe premaxillary&#8722;maxillary and dentary bones to different extents. The occurrence of BD in thedentary bone represented 58%, whereas premaxillary&#8722;maxillary BDs were observed in 14% ofthe studied specimens, while the complete rostrum (dentary, premaxillary and maxillary) wasaffected in 28% of the skulls. Dorsoventral axis BD was more frequent than lateral BD (48 and38%, respectively), and double BD was only observed in the dentary bone. Most of the BDobserved in this study could be classified as mild\/moderate, and we assume that it did not affectthe feeding activities of individuals; however, 2 specimens (<1%) showed a severe and complexcurvature that probably did affect them. The cause of these anomalies (natural or anthropogenicorigins) is unknown but may be related to important parasite loads, heavy metal and organic contaminantsand plastic ingestion that could affect the coastal dolphin in different ways. A moredetailed and thorough study of these cranial anomalies is necessary","palabras_clave":"Franciscana dolphin|Skull anomalies|Rostral bones deviations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4818,"idioma_id":4,"titulo":"Reactivation, inversion and basement faulting and thrusting in the Sierras Pampeanas of Córdoba (Argentina) during Andean flat-slab deformation","resumen":"The Sierras Pampeanas of Córdoba are the easternmost uplifted blocks caused by Andean foreland deformation, over 700 km from the Chile trench. This deformation started at c. 340 Ma through basement faults, thrusts and reactivation of normal faults of the Cretaceous rift during the opening of the Atlantic Ocean. Other older faults, major oblique lineaments, were also reactivated. Thermochronological and geothermobarometric data indicate that some topographic relief could have been Palaeozoic-Mesozoic relicts and not only produced by the Andean orogeny. Faults are partially controlled by the early Cambrian S2 metamorphic foliation, coincident with the curved fault traces at map scale. During Pliocene time, two deformation phases post-dating Miocene-Pliocene magmatism are recognized. Shallow seismicity data (c. 25 km depth) indicate that the Sierras de Córdoba accommodate Quaternary displacement. Magnetotelluric studies detect the interface between the Pampia terrane and the Río de la Plata craton. The role of the oblique lineaments in the nucleation and development of the Tertiary faulting has been little considered; they could be correlated with an old pan-Gondwanan trend. During the Cretaceous period these lineaments worked in a transtensive way, producing the uplift of high-grade rocks and segmentation of the mountain chain favouring the diachronous uplift along the ranges. Recently, both the brittle-ductile transition at c. 23 km depth and the crustal thickness have been determined by seismicity analysis. The oblique lineaments displace normally the Mohorovicic discontinuity. Main basement thrusts were probably rooted in the suture between the Pampia terrane and the Río de la Plata craton.","palabras_clave":"thick-skinned style|faulting|Andean deformation|Eastern Sierras Pampeanas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4820,"idioma_id":4,"titulo":"Electrochemical sensor for the determination of enterotoxigenic Escherichia coli in swine feces using glassy carbon electrodes modified with multi-walled carbon nanotubes","resumen":"Enterotoxigenic Escherichia coli F4 (K88) (ETEC F4) is one of the main etiologic agents involved in neonatal and post-weaning diarrhea in pigs,which generates significant economic losses due to high mortality and morbidity. An electrochemical sensor based on glassy carbon electrodes modified with a dispersion of multi-walled carbon nanotubes plus 1% Nafion?is proposed to detect and quantify ETEC F4 in stool samples of pigs. The preparation of samples was made by dilution in phosphate buffer solutions (PBSs), followed by centrifugation and inactivation of the supernatant in autoclave. An irreversible oxidation peak was observed by cyclic voltammetry at a potential close to 0.69 V. The best response was obtained for an optimum pre-concentration time of 10 min under forced convection conditions. The electrochemical response performed by squarewave voltammetrywas obtained in 1\/5000 fecal samples in PBS spiked with ETEC F4. A peak current vs. ETEC F4 concentration plot was constructed from successive additions of suspensions of ETEC F4 inactivated by autoclave. The limit of detection (LOD), the limit of quantitation (LOQ) and the relative standard deviation were 6 × 104 CFU mL&#8722;1, 2 × 105 CFU mL&#8722;1 and 20%, respectively. The developed electrochemical method is simple, fast and economical to quantify ETEC F4 in swine stool samples, making it useful for diagnosis monitoring in production facilities.","palabras_clave":"ESCHERICHIA COLI F4 (K88)|SWINE STOOL SAMPLES|MULTI-WALLED CARBON NANOTUBES|ELECTROANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4821,"idioma_id":4,"titulo":"Electrochemical immunosensor based on gold nanoparticles deposited on a conductive polymer to determine estrone in water samples","resumen":"This paper describes the development of a simple and sensitive electrochemical immunosensor (EI) to quantify estrone (E) in water samples. This EI does not require the sample pre-treatment, to label neither the antigen nor the antibody, and its detection format is based on the fact that E is co-substrate of the horse radish peroxidase (HRP). Therefore, the EI was constructed by immobilization of the anti-E monoclonal antibody (mAbE) on a glassy carbon electrode (GCE) modified with gold nanoparticles (AuNPs) electro-synthesized on a 1-naphtylamine polymer (pNap) film. This format reduced significantly the time of EI preparation. Water samples were spiked with known E concentrations, and then incubated on mAbE-AuNPs-pNap-GCE disk electrode. The electrochemical response was proportional to the amount of pyrocatechol (H2Q), another enzyme co-substrate, and inversely proportional to the amount of E presents in water samples. The immunosensor showed a linear range from 8 × 10&#8722;2 to 2 × 104 pg mL&#8722;1. The limit of detection (LOD) was 0.061 pg mL&#8722;1. Recovery percentages obtained were very good, with values of 98.20, 105.50, and 100.85% for 50, 100 and 200 pg mL&#8722;1, respectively. Tests were also conducted to evaluate the cross-reactive of E with other hormones of similar structure such as 17&#946;-estradiol, progesterone and estriol. The EI showed a high selectivity to determine E in the presence of these hormones. Thus, this EI is an attractive tool to determine E in water samples.","palabras_clave":"Inmunosensor|Estrone|Gold nanoparticles|Poly-1-naphtylamine|Square wave voltammetry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4822,"idioma_id":4,"titulo":"Topographical and Physicochemical Contrast in Photopatterned SU-8 Films for Microfabrication of Multilayer Structures","resumen":"During SU-8 standard photolithography process, a patterned topography is formed with a characteristic height profile produced by the different shrinkage of the UV exposed and masked regions. We study the change of wettability, film solubility, and topographic modifications on SU-8 films of different thicknesses and show its relevance in the formation of spinning-flow arrays on top layers made from positive photoresists. Also, considerable contrast in film solubility and surface energy as observed from contact angle measurements is produced. Interface diffusion of the photoresists was also observed and followed by Rutherford Back Scattering. We discuss the derivations of the mentioned effects concerning the limitations to multilayered microfabrication processes and possibilities to take advantage of the surface profiles obtained.","palabras_clave":"microfabricación|multicapas|epoxi|interdifusión"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4823,"idioma_id":4,"titulo":"ACTH modulates PTP-PEST activity and promotes its interaction with paxillin","resumen":"Adrenocorticotropic hormone (ACTH) treatment has been proven to promotepaxillin dephosphorylation and increase soluble protein tyrosine phosphatase(PTP) activity in rat adrenal zona fasciculata (ZF). Also, in-gel PTP assayshave shown the activation of a 115-kDa PTP (PTP115) by ACTH. In this context,the current work presents evidence that PTP115 is PTP-PEST, a PTP thatrecognizes paxillin as substrate. PTP115 was partially purified from ratadrenal ZF and PTP-PEST was detected through Western blot in bioactive samplestaken in each purification step. Immunohistochemical and RT-PCR studiesrevealed PTP-PEST expression in rat ZF and Y1 adrenocortical cells. Moreover, aPTP-PEST siRNA decreased the expression of this phosphatase. PKAphosphorylation of purified PTP115 isolated from non-ACTH-treated ratsincreased KM and VM. Finally, in-gel PTP assays of immunoprecipitated paxillin from controland ACTH-treated rats suggested a hormone-mediated increase in paxillin?PTP115 interaction, while PTP-PEST and paxillin co-localize in Y1 cells.Taken together, these data demonstrate PTP-PEST expression in adrenal ZF andits regulation by ACTH\/PKA and also suggest an ACTH-induced PTP?PEST?paxillin interaction.","palabras_clave":"ADRENAL ZONA FASCICULATA|ACTH|PROTEIN TYROSINE PHOSPHATASE|PAXILLIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4825,"idioma_id":4,"titulo":"Thinning of the Horcones inferior debris-covered glacier, derived from five ablation seasons by semi-continuous GNSS geodetic surveys (Mt. Aconcagua, Argentina)","resumen":"We report on ice movements changes in the Horcones Inferior Glacier (HIG), a major debris-covered glacier located in the southern sector of Mt. Aconcagua, Central Andes of Argentina. The HIG has been characterized by outstanding surge phenomena in the recent past, with the last episode registered in 2003. After a surge episode, the surface becomes highly unstable, with continuous ice degradation. We studied the response of the glacier surface in the post-surge stagnation period, based on six semi-continuous Global Navigation Satellite System (GNSS) stations distributed along the main axis of the glacier. Kinematic GNSS profiles were acquired over the surface, aiming to strengthen the GNSS measurements of elevation change. Our results show a horizontal surface displacement from 0.4 cmd, and a 0.8 cmd mean elevation reduction during the 2009-2014 period. GNSS profiles also show a velocity increase, ranging from -1.1 cmd in 2012 to -1.8 cmd in 2013. Changing surface velocities in the HIG may be related to the presence of a thick debris cover, in combination with faster glacier degradation due to thickness loss after the 2003 surge, and glacier-climate interaction.","palabras_clave":"GNSS|Debris-covered glacier|Change detection|Mt. Aconcagua."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4826,"idioma_id":4,"titulo":"Numerical simulations of the pseudoelastic effect in CuZnAl shape-memory single crystals considering two successive martensitic transitions","resumen":"Shape memory alloys (SMAs) with double martensitic transitions are potential candidates for highly effective, nonstandard mechanical damping systems. This paper presents a numerical model that can be used to simulate pseudoelasticity in systems with two successive martensitic transformations, such as adequately oriented CuZnAl, CuAlNi and CuAlBe single crystals. The model is based on stress vs. strain data obtained from the tensile test of a CuZnAl single crystal and is able to simulate the mechanical damping of SMAs with two successive martensitic transitions. The numerical model has been implemented as an algorithm and used to assess the mechanical damping capacity of a system based on CuZnAl SMA single crystals, considering the complete &#946;-18R-6R cycle. A numerical model with a single degree of freedom is used and the behavior of the SMA-based damper is analyzed both under free and forced oscillation conditions. The results obtained indicate that the alloy studied is a very effective mechanical damper.","palabras_clave":"NUMERICAL SIMULATIONS|SHAPE-MEMORY EFFECT|MARTENSITIC TRANSFORMATIONS|PSEUDOELASTICITY|CU-ZN-AL|SINGLE CRYSTALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4827,"idioma_id":4,"titulo":"A Birefringent Polarization Modulator: Application to Phase Measurement in Conoscopic Interference Patterns","resumen":"Conoscopic interferometry for crystal characterization is a very well known technique with increasing applications in different fields of technology. The advantage of the scheme proposed here is the introduction of a polarization modulator that allows the recovery of the phase information contained in conoscopic interferograms. This represents a real advantage since the most relevant physical information of the sample under study is usually contained in the phase of the fringe pattern. Moreover, this technique works successfully even when there are no visible fringes.The setup employed is a simple conoscopic interferometer where the elements under study correspond to two birefringent crystal slabs and a commercial mica wave plate. It allows the crystals to be characterized and the wave plate retardance to be measured as a function of the angle of incidence. The modulator itself consists of a single tiltable crystal plate which, by means of phase shifting techniques, permits the recovery of a phase map for each sample.&nbsp; It is inexpensive and it can be easily calibrated, so it works with a wide range of phase shifting interferometry algorithms. We show that our scheme is easily adaptable to algorithms that require either a low or high amount of interferograms.","palabras_clave":"Fringe analysis|Phase measurement|Polarization modulator|Birefringence|uniaxial crystal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4828,"idioma_id":4,"titulo":"Hormonal regulation of metabolism","resumen":"Given the current environment in most developed countries, it is a challenge to maintain a good balance between calories consumed and calories burned, although maintenance of metabolic balance is key to good health. Therefore, understanding how metabolic regulation is achieved and how the dysregulation of metabolism affects health is an area of intense research. Most studies are focused on the hypothalamus, which is a brain area that acts as a key regulator of metabolism. Among the nuclei that integrate the hypothalamus, the arcuate nucleus is one of the major mediators in the regulation of food intake. The regulation of energy balance is also a key factor to ensure the maintenance of any species because of the dependence of reproduction on energy stores. Adequate levels of energy reserves are necessary for proper functioning of the hypothalamus-pituitary-gonadal axis. This review discusses valuable data presented in the 2015edition of the International Workshop of Neuroendocrinology concerning the fundamental nature of the hormonal regulation of the hypothalamus and the impact on energy balance and reproduction.","palabras_clave":"PITUITARY|METABOLISM|SEXUAL DIFFERENTIATION|HORMONES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4829,"idioma_id":4,"titulo":"Ovule and megagametophyte development in selected species of Apeibeae and Grewieae (Malvaceae-Grewioideae) from South America and its systematic implications.","resumen":"Ovule and female gametophyte development in C. argutus, L. divaricata and T. semitriloba was analyzed. The ovules of all species are anatropous, bitegmic and crassinucellate mixed type. The micropyle of the studied species is formed by the outer integument (exostome). The ovule of L. divaricata differs from those of the other two species because the chalazal tissue expands forming a cap, giving rise to a wing in the seed.  All species present one hypostase. The megaspore mother cell gives rise to a linear megaspore triad in C. argutus and L. divaricata, whereas in T. semitriloba, triads and diads can be observed in the same ovule. The chalazal megaspore develops a seven-celled and eight-nucleate female gametophyte corresponding to the Polygonum type. The synergids of L. divaricata have hooks and a conspicuous filiform apparatus. The antipodal cells in C. argutus are persistent, whereas in the other species, they are small and ephemeral. This is the first embryological report of the Grewioideae subfamily, which contributes to the characterization of the studied genera. Then compare to the data known for the family are inferred embryological characters for the group.","palabras_clave":"CALLOSE FEMALE GAMETOPHYTE|CORCHORUS, LUEHEA, TRIUMFETTA|FEMALE GAMETOPHYTE|HYPOSTASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4830,"idioma_id":4,"titulo":"Inflationary gravitational waves in collapse scheme models","resumen":"The inflationary paradigm is an important cornerstone of the concordance cosmological model. However, standard inflation cannot fully address the transition from an early homogeneous and isotropic stage, to another one lacking such symmetries corresponding to our present universe. In previous works, a self-induced collapse of the wave function has been suggested as the missing ingredient of inflation. Most of the analysis regarding the collapse hypothesis has been solely focused on the characteristics of the spectrum associated to scalar perturbations, and within a semiclassical gravity framework. In this Letter, working in terms of a joint metric-matter quantization for inflation, we calculate, for the first time, the tensor power spectrum and the tensor-to-scalar ratio corresponding to the amplitude of primordial gravitational waves resulting from considering a generic self-induced collapse.&nbsp;","palabras_clave":"Cosmology|Inflation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4832,"idioma_id":4,"titulo":"Yerba mate (Ilex paraguariensis) waste and alginate as a matrix for encapsulation of N fertilizer","resumen":"In the present work the recycling of yerba mate (Ilex paraguariensis) industrial by-products into urea containing capsules is described as an environmentally friendly strategy for nitrogen release into the soil.Yerba mate is an infusion traditionally drunk in various countries of South America. An extensive physical characterization of the capsules is reported: Scanning Electronic Microscopy (SEM) and Energy Dispersive X-rays analysis (EDX), water gain and hygroscopicity, texture properties, measurement of waterabsorbency, Differential Scanning Calorimetry (DSC) and Fourier Transform Infrared Spectrometry (FTIR).Capsule matrix had a yerba mate powder content ranging from 30% to 77%. Nitrogen release from the capsule-fertilizer and the capsule degradation were studied using soil (~pH 7.50). All the results indicate that yerba mate powder capsules with urea, as an example of environmental friendly fertilizer, may be expectedto have wide applications for sustainable development of modern agriculture.","palabras_clave":"CAPSULES|YERBA MATE POWDER| UREA|CALCIUM ALGINATE|, BIODEGRADABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4833,"idioma_id":4,"titulo":"Monitoring the biodegradability of bisphenol A and its metabolic intermediates by manometric respirometry tests","resumen":"As a result of its wide usage in the production of polycarbonate plastics and epoxy resins, bisphenol A (BPA) is commonly detected in wastewaters. Recently,BPA became amajor concern because its adverse effects as an endocrine disruptor. In this work, the biodegradation kinetics of BPA and its metabolic intermediates4-hydroxyacetophenone (4HAP), 4-hydroxybenzaldehyde (4HB) and 4-hydroxybenzoic acid (4HBA) by BPA-acclimated activated sludge was studied using manometric respirometry (BOD) tests. For all the tested compounds, BOD curves exhibited two phases. In the first one, a fast increase of the oxygen consumption (OC)due to the active oxidation of the substrate was obtained.Then,when the substrate was exhausted, the endogenous respiration produced a slower increase of OC.A standard Monod-model with biomass growth was used to represent the OC profiles as a function of time. For all the tested compounds, a good agreement between the proposed model and the experimental data was obtained.According to their biodegradation rates, the tested compounds can be ordered as follows: BPA\\4-HAP  4HB\\4HBA. Because the oxidation rate of BPA by BPA-acclimated activated sludge limits the rate of the whole biodegradation pathway, the accumulation of metabolic intermediates 4HAP, 4HB, and 4HBA would be negligible. To calculate the dissolved oxygen (DO) concentration (C) during the BODtests, the oxygen transfer coefficient (kLa) of the BOD bottles was obtainedusing the sulfite method. Finally, a simple procedure to calculate the minimum DO concentration (Cmin) based on BOD data was developed. Calculation results demonstrated that under the tested conditions, C values were high enough as not to be the limiting substrate for the microbial growth.","palabras_clave":"BISPHENOL A  | ENDOCRINE DISRUPTOR   |ACTIVATED SLUDGE  | RESPIROMETRY   |MODELLING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4834,"idioma_id":5,"titulo":"Identificación y caracterización litológica mediante el uso del sensor ETM+ (LANDSAT 7). Caso de estudio: entorno del glaciar Upsala, Argentina","resumen":"El objetivo del presente estudio se enfoca en evaluar y analizar técnicas de procesamiento digital de imágenes aplicadas al sensor Enhanced Thematic Mapper Plus (ETM+), a fin de demarcar y caracterizar las litologías aflorantes en la cuenca del glaciar Upsala, Santa Cruz, República Argentina. El prolífico desarrollo tecnológico-espacial experimentado en las últimas décadas ha dado lugar a la generación, sin precedentes, de sensores remotos capaces de capturar información de la superficie terrestre y también al mejoramiento de las técnicas de procesamiento digital de imágenes; entre las que se destacan las bondadosas posibilidades en la detección y diferenciación de diversas cubiertas presentes en la superficie terrestre como glaciares, volcanes, vegetación, suelos, agua, tipos de rocas aflorantes, etc. Esta situación ha coadyuvado y posibilitado obtener promisorios avances en el mapeo y monitoreo de fenómenos naturales en el campo de las ciencias de La Tierra. La metodología propuesta, incluye correcciones radiométricas; eliminación de coberturas no deseadas; evaluación estadística de la combinación de bandas más óptima; aplicación del índice Z; discretización de la serie estadística mediante una clasificación no supervisada; tareas de vinculación de la clasificación espectral con muestras litológicas y un posterior muestreo de validación. A través del procesamiento digital de imágenes, se proveyeron valiosos resultados que fueron validados y arrojaron un 82% de confiabilidad en la clasificación propuesta, lo cual resulta óptimo teniendo en cuenta la diversidad de cubiertas presentes y la heterogeneidad litológica que compone a cada píxel (30 m).  Además, los resultados fueron validados con mapas geológicos pre-existentes obteniendo buena concordancia.","palabras_clave":"SENSOR ETM+|CLASIFICACIÓN NO SUPERVISADA|SENSORES REMOTOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4835,"idioma_id":4,"titulo":"HmuS and HmuQ of Ensifer\/Sinorhizobium meliloti degrade heme in vitro and participate in heme metabolism in vivo","resumen":"Ensifer meliloti is a nitrogen-fixing symbiont of the alfalfa legume able to use heme as an iron source. The transport mechanism involved in heme acquisition in E. meliloti has been identified and characterized, but the fate of heme once inside the cell is not known. In silico analysis of E. meliloti 1021 genome revealed no canonical heme oxygenases although two genes encoding putative heme degrading enzymes, smc01518 and hmuS, were identified. SMc01518 is similar to HmuQ of Bradyrhizobium japonicum, which is weakly homologous to the Staphylococcus aureus IsdG heme-degrading monooxygenase, whereas HmuS is homolog to Pseudomonas aeruginosa PhuS, a protein reported as a heme chaperone and as a heme degrading enzyme. Recombinant HmuQ and HmuS were able to bind hemin with a 1:1 stoichiometry and displayed a Kd value of 5 and 4 µM, respectively. HmuS degrades heme in vitro to the biliverdin isomers IX-&#946; and IX-&#948; in an equimolar ratio. The HmuQ recombinant protein degrades heme to biliverdin IX-&#948; only. Additionally, in this work we demonstrate that humS and hmuQ gene expression is regulated by iron and heme in a RirA dependent manner and that both proteins are involved in heme metabolism in E. meliloti in vivo.","palabras_clave":"Rhizobia|Heme-oxygenase|Iron Metabolism|Heme-degradation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4836,"idioma_id":4,"titulo":"CHANGES IN RENAL WT-1 EXPRESSION PRECEDING HYPERTENSION DEVELOPMENT","resumen":"Hypertension is a public health problem mostly unknown causes, and where strong hereditary genetic alterations, have not been fully elucidated. However, the use of experimental models, have provided valuable information. Interestingly, recent evidences suggest that alterations in keys nephrogenic factors such Wilms tumor 1 factors (WT-1), could contribute to the development of hypertension. Aims: To evaluate the expression of WT-1 and related genes in the nephrogenic process associated with the development of hypertension as well as the corresponding anatomical and functional correlation. Male spontaneously hypertensive rats (SHR) were evaluated weekly from birth until the eighth week of life. We established the relationship of renal weight\/total body weight without values significantly different. These data were compared with apoptosis, fibrosis, number and size of the glomeruli. The elevation of systolic blood pressure was significant since the sixth week. Biochemical values were slightly modified. Histology showed an increase in deposits of collagen fibers since the 4th week. Additionally, decreased expression of WT-1, heat shock protein 70 (Hsp70) and vitamin D receptors (VDR) since the fourth week in SHR were shown. Finally, we demonstrated ultra-structural damage to mitochondria since the fourth week of life. Our results would suggest an unprecedented link, possibly regulatory mechanistic, of WT-1 on nephrogenic alteration processes and their relationship with hypertension. If so, it could suggest that deregulation in the expression of WT-1 and its impact on the nephrogenesis induction; they could be crucial in understanding the development and maintenance of hypertension.","palabras_clave":"Hipertensión|Nefrogenesis|vitamina D"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4837,"idioma_id":4,"titulo":"Sperm conjugation in mammal reproductive function: Different names for the same phenomenon?","resumen":"SUMMARYIn many mammalian and non-mammalian species, mature sperm interact within the female reproductive tract or inside the epididymal lumen using cohesive forces. This phenomenon, known as ?sperm conjugation,? is sometimes confused with sperm agglutination, which is the result of the interaction of epididymal or ejaculate spermatozoa upon release into culture medium. In addition to ?agglutination,? the terms ?association,? ?rouleaux,? or ?rosettes? are employed interchangeably to describe the conjugation phenomenon, which inevitably causes confusion due to the non-unifying nomenclature. This variety of descriptions is likely due to a poor understanding of the molecular mechanisms involved in such conspicuous cell-cell interaction as well as the different morphologies that result from such interactions among species. Here, we summarize the published data regarding mammalian sperm conjugation, considering the organisms in which sperm interaction was observed; the particular terminology employed; findings regarding the components that enable sperm to adhere; sperm behavior when deposited in the female reproductive tract; and hypotheses formulated to clarify the biological function and, when known, the mechanisms for sperm interaction. We also propose a new classification system for this phenomenon that might clearly unify the criteria used to describe this behavior. Mol. Reprod. Dev. 2016. © 2016 Wiley Periodicals, Inc.","palabras_clave":"Espermatozoides|Conjugados de espermatozoides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4838,"idioma_id":5,"titulo":"ANALISIS POLINICO DE MIELES INMADURAS EN EL SECTOR OESTE DE LAS YUNGAS DE JUJUY, ARGENTINA","resumen":"Con el objetivo de conocer los recursos nectaríferos utilizados por Apis mellifera L. en el sector oeste de las Yungas, se examinaron 14 muestras de miel inmadura y dos de miel madura correspondientes a los períodos apícolas 2011?2012 y 2012?2013, pertenecientes a un apiario de la localidad de Tilquiza (Jujuy). Las muestras fueron procesadas de acuerdo a las técnicas melisopalinológicas convencionales con posterior acetólisis. Se identificaron un total de 53 tipos polínicos pertenecientes a 31 familias botánicas, siendo las familias con mayor riqueza polínica Asteraceae y Fabaceae. Se identificaron como recursos dominantes a Crinodendron tucumanum en setiembre de2011, Blepharocalyx salicifolius en noviembre y diciembre de 2011 y al tipo Scutia\/Condalia en octubre de 2012. Como aportes secundarios se registraron a Allophylus edulis, Zanthoxylum, Parapiptadenia excelsa, Baccharis, Gnaphalium, Eucalyptus y tipo Cissus, destacándose la influencia de la flora nativa de las Yungas. A partir de este estudio se pudo conocer la dinámica del ingreso de néctar a la colmena y valorar aquellos recursos que no se encuentran presentes en las mieles maduras y que son de importancia para el desarrollo de la colmena.","palabras_clave":"NECTAR|MELISOPALINOLOGIA|APIS MELIFERA|YUNGAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4839,"idioma_id":5,"titulo":"Concentración de nitratos en dos tipos comerciales de lechuga (Lactuca sativa L.) cultivada con distintas fuentes nitrogenadas","resumen":"Los nitratos (NO3-) ingeridos por el hombre pueden convertirse a nitritos (NO2-) formando metahemoglobina y nitrosaminas carcinogénicas. La lechuga se encuentra entre las hortalizas con mayor capacidad de acumulación de nitratos. El objetivo del estudio fue estudiar la respuesta del cultivo de lechuga a dosis crecientes de nitrógeno aplicado como urea y al agregado de lombricompuesto, sobre la concentración de nitratos en dos tipos comerciales (hoja suelta y mantecosa). Se realizaron dos ensayos en simultáneo a campo en dos épocas de producción: otoño-invierno y primavera. En el primero se incorporó abono orgánico (24 t ha-1 de lombricompuesto- L1) y se comparó un testigo sin abonar (L0). El segundo ensayo comparó un testigo sin fertilizar (N0) con 75 kg N ha-1  (N1) y 150 kg N ha-1(N2) aplicado como urea. El diseño experimental fue en bloques completamente aleatorizados con cuatro repeticiones. Durante el cultivo y a la cosecha se determinó la concentración de NO3- por colorimetría. Se observó un nivel decreciente de nitratos para ambos ensayos durante el período invernal. El genotipo influyó en la concentración de nitratos, según tratamientos y épocas. Para el tratamiento con lombricompuesto se evidenció una mayor proporción de nitratos para Brisa (hoja suelta) en ambas épocas. En otoño-invierno, Brisa reflejó respuesta a la fertilización con urea mostrando mayores niveles de nitratos a cosecha con respecto a Daguan. En primavera no hubo  respuesta a la fertilización y solo el tratamiento N2 en Brisa presentó mayores niveles de nitratos en hoja a cosecha.","palabras_clave":"fertilización química|urea|lombricompuesto|compuestos carcinogénicos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4840,"idioma_id":5,"titulo":"Sustentabilidad y Derechos Humanos: un abordaje desde las nuevas tecnologías de información y comunicación en la universidad","resumen":"En este trabajo compartimos la experiencia de construcción de un espacio destinado a los Derechos Humanos dentro del portal educativo ?Sustentabilidad en los sistemas agropecuarios y naturales?, abierto al público por la Facultad de Agronomía de laUniversidad de Buenos Aires. El concepto ?desarrollo sustentable? está incluido en la enseñanza de las carreras que ofrece la institución. En la asignatura ?Derechos Humanos? se aborda a través del derecho a un ambiente sano, a la alimentación y altrabajo decente. El aprendizaje se basa en actividades semipresenciales, con acceso a charlas a cargo de expertos y debates en foros virtuales. El Grupo de Estudio y Trabajo en Derechos Humanos, a cargo del curso, consideró que podía y debía difundir los materiales del curso para otro público, lo que pudo hacerse a través del portal. La complejidad de la sustentabilidad, la necesidad de un abordaje interdisciplinario, y su relevancia en la actividad profesional justificaron la tarea realizada.","palabras_clave":"tecnologia de la informacion y comunicacion |educacion universitaria|agronomia|innovacion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4842,"idioma_id":4,"titulo":"EFFECT OF LIGHT ENVIRONMENT ON HARVEST QUALITY AND POSTHARVEST BEHAVIOR OF MINIMALLY PROCESSED SWEET PEPPER","resumen":"The aim of this study was to evaluatethe effect of two light environments on two maturity stages of sweetpepper&nbsp; and the postharvest behavior ofminimally processed product using passive modified atmosphere. The size andfruit morphology were measured at harvest. Overall visual quality, gasconcentration inside the bags, weight loss, soluble solids, firmness,electrolyte leakage, carotenoids and ascorbic acid were determined during thestorage period. Results showed that quality parameters were affected byradiation and processing. Shaded pepper fruitsshowed higher water content and lower size compared to fruits exposed to fullradiation. Fruit nutritional value was maintained, but after six days was notmarketable. Visual quality of intact fruit was not affected without differencesbetween treatments. Shelf life processed red fruits as julienne was reduced,being the worst performing during storage. Post-harvest visual quality was inrelation to gases composition (O2 and CO2) inside thetrays.","palabras_clave":"Capsicum sp,   |radiation,|modified atmosphere,|appearance, |nutrition value."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4844,"idioma_id":4,"titulo":"Coastal aquifer hydrodynamics and salinity in response to the tide. Case study in Lisbon, Portugal","resumen":"The variability in dynamics and salinity of the coastal alluvial aquifer on which the city of Lisbon is located was evaluated. Such an evaluation was based on the analysis of level and groundwater electrical conductivity fluctuations depending on the tide in the Tagus River. The results obtained made it possible to recognize three sectors. First, a littoral sector, where the variations in level and salinity are larger on the coast and decrease towards the innermost sections of the alluvial fan. Then, a sector close to the docks, where there is greater dynamic and salinity variability than in the coastal sectors, as the excavations of the docks favour the tidal propagation towards the aquifer. And finally, a sector located towards the apex of the alluvial fan, associated with the dynamics of the stormwater channel. In this sector, the largest periodical water table fluctuations in the aquifer occur, since the freshwater that cannot drain towards the river enters the aquifer at high tide, causing a slight decrease in salinity content. On the basis of these results, conceptual models of hydrogeological behaviour were used to describe the spatial and temporal variations in the hydrodynamic and salinity characteristics of groundwater.","palabras_clave":"COASTAL AQUIFER|TIDAL HYDRODYNAMICS|SALINITY VARIABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4845,"idioma_id":4,"titulo":"Identification of palaeo-seawater intrusion in groundwater using minor ions in a semi-confined aquifer of the Río de la Plata littoral (Argentina)","resumen":"The hydrochemistry of minor elements and traces such as bromide, lithium, strontium, uranium and selenium, togetherwith the chemical analysis ofmajor ions, has been used in the study of salinization process. This processBoccurs in a semi-confined aquifer that corresponds to a Pliocene?Pleistocene fluvial environment. The semi-confined aquifer is located in the littoral of the cities of Ensenada and Berisso, in the region of the middle Río de la Plata estuary, Argentina. Groundwater salinization was detected in the semi-confined aquifer in the coastal plain area, with salt contents that increase from the loess plain towards the river. The content of major ions that predominate in sea water (Cl&#8722;, Na+ and Mg2+), as well as the Cl&#8722;\/Br&#8722; and U vs. Cl&#8722; ratios, demonstratesthat such salinization is related to seawater,which shows no correspondencewith estuarywater. In the salinized area, Li, Sr and Se enrichments occur, and are used as tracers of the average time that a substance remains in solutionin sea water in the aquifer. The study of suchminor ions togetherwith the geological evolution of the area made it possible to recognize that the saltwater in the semi-confined aquifer corresponds to a palaeo-intrusion ofsea water associated with the Pleistocene?Holocene ingressions caused by the climate changes occurring duringthe Quaternary.","palabras_clave":"SALINIZATION|HYDROGEOCHEMISTRY|PALAEO SEAWATER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4846,"idioma_id":4,"titulo":"Evaluation of the rate of abiotic and biotic degradation of oxodegradable polyethylene","resumen":"The recent introduction of oxo-degradable additive in the Argentinean market has motivated the study of the effect of abiotic (temperature and ultraviolet (UV) radiation) and biotic (aerobic in compost) degradation on the structure and mechanical behavior of films of polyethylene (PE) and oxo-degradablepolyethylene (PE+AD).Physico-chemical tests show that the failure strain and the carbonyl index of degraded PE and PE+AD samples depend on the UV irradiation dose. Furthermore, the additive plays a crucial role in the degradation and subsequent decay of the molecular weight.It was observed that, for the same dose, the most deteriorated material was the one exposed to the lowest irradiance, emphasizing the importance of the time of exposure to UV radiation. The ratio between the irradiance and the critical dose, is a characteristic time associated to the sharp decay on the failure strain. The critical dose decreases significantly when increasing the temperature of the photodegradation assay.PE is more susceptible to thermal degradation than PE+AD; the latter only degrades under thermal aging at the highest temperature.Initially biotic degradation in compost showed an increasing production of carbon dioxide for both previously UV-degraded and untreated PE+AD. It is also remarkable that UV-degraded samples of PE and PE+AD with differences in their abiotic degradation level, reached the same final biotic degradation level. It was observed that although the additive increased the abiotic photodegradation, the molecular weight reduction in compost was not enough to reach the maximum biotic degradation level established by international standards for biodegradable materials.","palabras_clave":"oxodegradables|biodegradación|degradación abiótica|propiedades mecánicas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4847,"idioma_id":4,"titulo":"Synergism in anticorrosive paints","resumen":"The present work depicts synergism anticorrosive behaviour between zinc hypophosphite and zinc phosphatein a commercial pigment mixture. Also, the performance of anticorrosive paints was evaluated. Synergismanticorrosive behaviour was evaluated by corrosion potential and linear polarization measurements in pigment suspensions.The protective layer obtained with this pigment mixture was investigated by scanning electron microscopy(SEM). Then, the anticorrosive properties of the pigment were assessed by incorporating it into alkyd and epoxypaints which were evaluated by salt spray test and electrochemical noise technique. The morphology and the natureof the protective layer grown under the paint film were also studied by SEM. Experimental results showed thatimproved anticorrosion protection is achieved in paints with reduced zinc phosphate contents as a consequence ofthe synergistic interaction between zinc hypophosphite and the other components of the pigment mixture. The electrochemicalnoise technique proved to be adequate to monitor corrosion in painted panels and is able to detectcorrosion under the paint film from very early stages. This paper identified the need to study synergism betweenanticorrosive pigments to try to reduce the phosphate content in anticorrosive paints.","palabras_clave":"PIGMENTS|PAINTS|CORROSION PROTECTION|SYNERGISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4848,"idioma_id":5,"titulo":"El Dios de Leibniz a contrapelo de la distinción entre deber y querer","resumen":"En este artículo, presentamos primero un análisis de la proposición 'Dios elige siempre lo mejor', a fin de indagar qué tipo de necesidad cabe atribuírsele. Dado que Leibniz rechaza la necesidad geométrica en el ámbito de la libre elección divina, examinamos luego qué clase de relación hay entre el entendimiento y el bien, es decir, si corresponde prioritariamente a la razón juzgar sobre el bien o lo mejor. Del mismo modo, analizamos a continuación el vínculo que liga a la voluntad con el bien, por cuanto éste último parece ser, al mismo tiempo, el objeto natural de la voluntad. En ambos casos, el análisis se aplica tanto a Dios como a las criaturas racionales. Una aparente inconsistencia parece suscitarse entre un entendimiento y una voluntad que 'se disputan' el mismo objeto (el bien). Nuestro objetivo es mostrar que, aunque la prioridad de examinar sobre el bien pueda recaer en una u otra facultad, según el contexto escritural, la cuestión carece de sentido al aplicársela a Dios. En suma, la ambivalencia se diluye cuando advertimos que ello resulta de una ficción humana, a saber que hay un hiato entre deber y querer.","palabras_clave":"Principio de lo mejor|Bien real|Bien aparente|Paradoja de las facultades"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4849,"idioma_id":5,"titulo":"Desastres y catástrofes: algunos apuntes para la consideración de políticas públicas","resumen":"El presente artículo intenta dar cuenta de algunas claves para la consideración de políticas públicas a partir de la tarea que realizamos desde una universidad nacional. Además, puntualizaremos algunos aspectos que, desde una perspectiva psicosocial, entendemos hacen al cuidado del trabajador\/a frente a desastres o catástrofes. Lo hacemos como equipo interdisciplinario de trabajo de la Universidad Nacional de Entre Ríos, donde investigamos e intentamos poner en acto nuestra producción y experiencia, acompañando y capacitando a profesionales y voluntarios. Tratamos de interactuar promoviendo una perspectiva crítica y reflexiva que nos permita, a su vez, nutrir el proceso investigativo. De experiencias de extensión, nacen proyectos de investigación, lo cual fortalece ambas funciones,que a su vez se nutren de la docencia y alimentan a esta. ISSN: 2469-2417","palabras_clave":"DESASTRES|POLÍTICAS PÚBLICAS|INTERVENCIÓN|PROFESIONAL|PSICOSOCIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4850,"idioma_id":4,"titulo":"DES-ACYL GHRELIN DIRECTLY TARGETS THE ARCUATE NUCLEUS IN A GHRELIN-  RECEPTOR INDEPENDENT MANNER AND IMPAIRS THE OREXIGENIC EFFECT OF GHRELIN","resumen":"Ghrelin is a stomach-derived octanoylated peptide hormone that plays a variety of well-established biological roles acting via its specific receptor known as growth hormone secretagogue receptor (GHSR). In plasma, a des-octanoylated form of ghrelin, named des-acyl ghrelin (DAG), also exists. DAG is suggested to be a signalling molecule that has specific targets, including the brain, and regulates some physiological functions. However, no specific receptor for DAG has been reported until now, and, consequently, the potential role of DAG as a hormone has remained a matter of debate. In the present study, we show that DAG specifically binds to and acts on a subset of arcuate nucleus (ARC) cells in a GHSR-independent manner. ARC cells labelled by a DAG fluorescent tracer include the neuropeptide Y (NPY) and non-NPY neurones. Given the well-established role of the ARC in appetite regulation, we tested the effect of centrally administered DAG on food intake. We found that DAG failed to affect dark phase feeding, as well as food intake, after a starvation period; however, it impaired the orexigenic actions of peripherally administered ghrelin. Thus, we conclude that DAG directly targets ARC neurones and antagonises the orexigenic effects of peripherally administered ghrelin.","palabras_clave":"DES-ACYL GHRELIN RECEPTOR|HYPOTHALAMUS|ACYL-GHRELIN|FOOD INTAKE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4851,"idioma_id":5,"titulo":"MAPA BASE MORFOPEDOLÓGICO MEDIANTE UN SIG DEL DEPARTAMENTO GENERAL SAN MARTÍN, CÓRDOBA, ARGENTINA","resumen":"Cambios en el uso y manejo de la tierra determinaron en Argentina un fuerte impacto sobre recursos naturales, en especial sobre la Calidad de Suelo. La planificación física y ambiental constituye una de las principales vías para la solución de estos problemas por su función como soporte de las actividades humanas. En el departamento General San Martín, Córdoba no se dispone de una cartografía morfopedológica. El objetivo de este trabajo fue construir el mapa base morfopedológico para evaluar la calidad de suelo y desarrollar una planificación de agroecosistemas con prácticas de manejo ambientalmente sustentable. En base a la metodología mixta de Unidades Integradas -geomorfología, suelos, Imágenes satelitales Landsat 8- y gvSIG 1.12.0 se elaboró un mapa escala 1:50.000. Los resultados indican que el departamento General San Martín presenta tres grandes ambientes, paleoaluvial al norte, fluvial en el sector central (río Ctalamochita y arroyo Cabral) y drenaje en araña al sur (arroyos, cañadas, bañados y lagunas permanentes y temporarias) los cuales constituyen cinco Unidades Ambientales Integradas. Se considera imprescindible continuar con estudios a mayor detalle en las unidades morfopedológicas como base fundamental para una planificación sustentable del departamento General San Martin, Córdoba.","palabras_clave":"UNIDADES AMBIENTALES |GV SIG|CALIDAD DE SUEOS|PLANIFICACION TERRITORIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4853,"idioma_id":4,"titulo":"Diversification patterns of the CES clade (Tribes Cremolobeae, Eudemeae, Schizopetaleae: Brassicaceae) along Andean South America.","resumen":"Dated molecular phylogenies show that the Andean uplift had a major impact on South American biodiversity. For many Andean groups, accelerated diversifications (radiations) have been documented. However, not all Andean lineages appear to have diversified following the model of rapid radiation, particularly in the central and southern Andes. Here, we investigated the diversification patterns for the largest South American-endemic lineage of Brassicaceae, composed of tribes Cremolobeae, Eudemeae, and Schizopetaleae (CES clade). Species of this group inhabit nearly all Andean biomes and adjacent areas such as the Atacama-Sechura desert, the Chilean Matorral, and the Patagonian Steppe. First, we studied diversification times and historical biogeography of the CES clade. Second, we analysed diversification rates through time, lineages, and associated life forms. Results demonstrate that early diversification of the CES clade occurred in the Early-Mid Miocene (around 12?19 Mya) and involved the central Andes, the southern Andes together with the Patagonian Steppe, and the Atacama-Sechura desert. The Chilean Matorral and northern Andes were colonised subsequently in Early Pliocene (4?5 Mya). Diversification of the CES clade was recovered as a gradual process without any evidence for rate shifts or rapid radiation, in contrast to many other Andean groups analysed so far. Diversification time\/rates and biogeographic patterns obtained for the CES clade are discussed and compared with patterns and conclusions reported for other Andean plant lineages.","palabras_clave":"Andes|Atacama desert|biogeography|Chilean Matorral|diversification rates|molecular dating|Patagonian Steppe"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4854,"idioma_id":4,"titulo":"Opposite oleic acid responses to temperature in oils from the seed and mesocarp of the olive fruit","resumen":"Olive oil is mostly extracted from themesocarp (~95%) of the fruit with the seed (endosperm and embryo, ~5%)containing little oil. There arecorrelative and manipulative evidence that temperature modulates fruit oilcontent and fatty acid composition of the oil from the whole fruit (i.e., withno distinction being made between oils derived from each oil-bearing structure)of olive. Notably, oleic acid concentration of olive oil decreases as fruitmean growth temperature increases. This response in the olive fruit is oppositeto that documented in annual oil-seed crops such as sunflower and soybean. The objectives of the present study were: i)compare temperature effects on fatty acid composition of oil derived from seedand from mesocarp ; ii) Analyzed temperature effects on seed and mesocarp dryweights and oil concentrations. To do this, fruiting brancheswere enclosed in transparent plastic chambers with individualized temperaturecontrol. Temperature was manipulated during the seed growth (period A) andduring the second half of mesocarp growth (period B) subphases. &nbsp;In both periods, the oleic acid proportion inmesocarp oil decreased as temperature increased, and was accompanied byincreases of palmitic acid, linoleic and linolenic acids. Mesocarp dry weight didnot respond significantly to temperature, but mesocarp oil concentration fellsignificantly as temperature increased. Seed dry weight, oil concentration andfatty acid composition exhibited responses to temperature during period A only,with seed dry weight increasing&nbsp; between20 and 25°C with a sharp decrease at higher temperature, and oil concentration linearlyfalling 1.2% per °C. In contrast, seed oil oleic acid percentage increasedbetween 20 and 28°C, and fell slightly with higher temperature. Palmitic andstearic acids in seed oil increased sigmoidally with temperature, whilelinoleic acid decreased sigmoidally. Oleic acid percentage showed oppositeresponses in the seed and the fruit mesocarp.&nbsp;The response of the seed was similar to those observed in oil fromembryos of annual oil-seed crops, although the abrupt fall in palmitic andstearic acid with temperature &gt;25 °C seems to be distinctive for olive seedoil.","palabras_clave":"OLIVE OIL|OLEIC ACID|LINOLEIC ACID|STEARIC ACID|INCREASING TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4855,"idioma_id":5,"titulo":"Delimitación de parches de uso del suelo en la zona periurbana de Villa María, Córdoba, Argentina","resumen":"Las zonas periurbanas cuentan con una gran dinámica detransformación entre lo rural y lo urbano, donde se despliegancomplejos fenómenos ecológicos y sociales. El presente trabajoplanteó como objetivo establecer parches según el uso del sueloen la zona periurbana de Villa María, Córdoba, Argentina. Setuvieron en cuenta las condiciones geomorfológicas, ecológicasy normativas a fin de establecer las bases para la generación deindicadores ambientales. La metodología utilizada consistió enel procesamiento de imágenes satelitales mediante el programagvSIG y relevamiento a campo. Se delimitó la zona periurbanarealizando el cruzamiento de datos entre el relevamiento, lasnormativas y el marco teórico de ocupación del territorio. Seobtuvieron 19 parches de uso de suelo y se estableció la predominanciade los mismos.En Villa María, las políticas, planes y programas relacionados aluso del territorio periurbano carecen del conocimiento de lasposibilidades y limitaciones ambientales que presentan estosecosistemas. Por ello, esta investigación generó información valiosapara determinar la calidad ambiental y poder dar soporte anormativas que establezcan el uso del territorio para minimizarlos impactos negativos y considerar los servicios ambientales","palabras_clave":"PERIURBANO|VILLA MARÍA|CALIDAD AMBIENTAL|USO DEL SUELO|PARCHES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4856,"idioma_id":4,"titulo":"Let the music be your master: Power laws and music listening habits","resumen":"Music preferences have long been studied owing to their importance in the fields of psychology and sociology. However, previous efforts seldom focused on people?s deliberate choices of music in everyday life. In this study, we aimed to analyze music listening behaviors using personal records of music listening activity. We obtained the history of songs listened to by 50 different users of the online database system Last.fm, spanning on average five years of activity. With the use of this data set, we are able to confirm that the number of songs reproduced per artist follows a truncated power-law distribution. The scaling parameter of the distribution varies considerably among users, providing a metric that characterizes the way in which different people explore music. We propose that this pattern is consistent with a preferential attachment model, according to which the probability of listening to a given artist at a given time is proportional to the frequency to which the artist was listened to in the past. These results provide new insight regarding the way in which individual music preferences are built.","palabras_clave":"MUSICA|PSICOLOGIA|PREFERENCIAS|DSITRIBUCION POTENCIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4857,"idioma_id":5,"titulo":"Sociedad y ambiente en el territorio periurbano de Villa María","resumen":"El Ambiente es el entorno vital constituido por elementos físicos, biológicos, económicos, sociales, culturales y estéticos que interactúan entre sí, con el individuo y la comunidad, determinando la forma, el carácter, el comportamiento y la supervivencia de ambos.El avance de las urbanizaciones sobre los espacios rurales es un proceso continuo y constante que lleva consigo conflictos socioambientales. Es por ello, que el área periurbana se debería concebir como parte de un socioecosistema, que cuenta con una gran dinámica de transformación. Las políticas, planes y programas relacionados al uso de este territorio de transición no cuentan con el conocimiento de las posibilidades ambientales y las limitaciones que presentan los ecosistemas. Por ello el trabajo tiene como objetivo evaluar la relación entre la sociedad y el ambiente en función de los usos del suelo y su dinámica de interacción en la zona periurbana de la Ciudad de Villa María (Córdoba).La metodología de análisis es a partir del abordaje de la constitución sistémica de objetos y acciones en casos particulares de estudio, tales como, loteos recientes, escuela granja, ladrilleras, entre otros. Los resultados obtenidos hasta el momento permiten establecer que en las áreas consideradas se desarrollan 17 tipos de uso del suelo, los cuales deben ser incorporados en la normativa municipal contemplando las condiciones de los objetos, sus sistemas de acciones y las condiciones ambientales presentes.El estudio de los objetos y los sistemas de acciones de los componentes en la zona periurbana de la Ciudad de Villa María (Córdoba), constituye una herramienta integral que aporta conocimientos desde diferentes disciplinas para el entendimiento y a la generación de normas en el territorio.","palabras_clave":"Viilla María|Periurbano|Desarrollo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4858,"idioma_id":4,"titulo":"Population dynamics of two invasive amphipods in the Southwestern Atlantic: Monocorophium acherusicum and Ericthonius punctatus (Crustacea)","resumen":"Harbours are important sites for the containment and dispersal of invasive species throughout the world, so the study of life history traits of species is important to understand the success of their invasion and their potential effects on the habitat. In recent years several invasive species have been reported in Argentinian harbours; however, studies of the ecology and life history of these species are scarce. We studied the population dynamics and reproductive biology of Monocorophium acherusicum and Ericthonius punctatus, in order to update the published information on the introduced amphipods in Mar del Plata harbour. Both species showed a seasonal pattern characterized by high densities in warmer months, related to the highest reproductive activity and the increase of recruitment in summer and early autumn, and lower densities in the cold season. The sex ratio was always female-biased and the number of eggs carried by females was positively correlated to the size. The present study suggests that both species have colonized Mar del Plata harbour successfully, showing viable populations (cohorts of juveniles, males, females and ovigerous females). This work provides the basis for monitoring the impact generated by introduced amphipods over the existing fauna.","palabras_clave":"harbour|invasive species|amphipods"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4859,"idioma_id":5,"titulo":"Osteología craneal de Liolaemus azarae Avila 2003 (Squamata: Liolaemidae): aportes a la variación morfológica del género","resumen":"Con el objetivo de aportar información sobre la variación de los caracteres osteológicos en el género Liolaemus, examinamos siete individuos de Liolaemus azarae tratados con la técnica de diafanización y tinción diferencial de cartílago y hueso. Describimos el estado de quince caracteres craneales y comparamos ocho elementos óseos con dos especies del grupo wiegmanni: L. occipitalis y L. lutzae. Liolaemus azarae se distingue de estas últimas por siete caracteres","palabras_clave":"CRÁNEO|CARACTERES|VARIACIÓN|LIOLAEMUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4860,"idioma_id":4,"titulo":"The Toarcian Oceanic Anoxic Event (Early Jurassic) in the Neuquen Basin, Argentina: a reassessment of age and carbon-isotope stratigraphy","resumen":"The Toarcian oceanic anoxic event (T-OAE) is recorded by the presence of globally distributed marine organic carbon¨Crich black shales and a negative carbon isotope shift, with ¦Ä13Corg values as low as &#8722;33¡ë, interrupting an overarching positive excursion. Here we present new biostratigraphic data and high-resolution ¦Ä13Corg data from two Southern Hemisphere localities: Arroyo Serrucho in the north and Arroyo Lapa in the south of the Neuqu¨¦n Basin, Argentina. Previous studies at these localities aimed to provide an accurate numerical age for the T-OAE and characterization of its carbon isotope stratigraphy. The new carbon isotope data and ammonite biostratigraphy presented here from Arroyo Serrucho show the T-OAE to be recorded lower in the section than supposed by previous authors, thus calling into question the published age of the T-OAE in this section. A newly investigated exposure at Arroyo Lapa North shows a complex carbon isotope record with at least three high-amplitude fluctuations in the hoelderi zone (equivalent to the serpentinum zone in northwestern Europe), with ¦Ä13Corg values of &lt;&#8722;28¡ë, and two intervening positive isotope excursions, with ¦Ä13Corg values around &#8722;24¡ë. At Arroyo Lapa South, the characteristic major stepped negative carbon isotope excursion is recorded, with ¦Ä13Corg values of &lt;&#8722;30¡ë and total organic-carbon contents increasing to 11%; above this level an erosional surface of a submarine channel truncates the section. These new data are globally correlative and unambiguously illustrate the global reach of the T-OAE.","palabras_clave":"Chimiostratigraphy|Toarcian|TOAE|Neuquen Basin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4861,"idioma_id":5,"titulo":"Desigualdades regionales en planifi caciones desarrollistas. Una revisión del proceso de territorialización de Río Negro 1958?1976","resumen":"El estudio indaga en la dinámica de construcción de fronteras y diferencias al interior de la provincia de Río Negro, tomando como punto de inflexión la provincialización en 1958. Se busca evidenciar cómo se continuó con la fragmentación espacial de los años previos, profundizando y modificando las escisiones al restringir lo provincial a los intereses de ciertos sectores regionales.Para ello abordaremos un conjunto de fuentes escasamente analizadas: la Constitución provincial redactada en 1957 y los planes de desarrollo de los gobiernos entre 1958 y 1976. Avanzaremos en los debates asociados a la construcción jerárquica de las regiones, ejemplificando cómo la transformación espacial habilita, paradójicamente, la asunción del espacio no sólo como neutro, sino como marco justificado de una integración social desigual, que para el año 1973 ya comienza a plantearse en términos de determinismo geográfico, pues las desigualdades se justifican en descripciones del paisaje.","palabras_clave":"Desarrollismo|Planificación|Río Negro|Paisaje|Jerarquía"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4862,"idioma_id":4,"titulo":"'Karyotypes of four species of Xenodontini snakes (Serpentes: Dipsadidae) and implications for taxonomy'","resumen":"The karyotypes of four Xenodontini snake species, Lygophis dilepis, L. meridionalis, L. flavifrenatus and L. anomalus, are here described for the first time. We studied specimens from northeastern Argentina using conventional and silver (Ag-NOR) staining. While the typical ophidian karyotype is 2n = 36, we found that the karyotype of the studied species is 2n= 34, with metacentric and submetacentric chromosome pairs. The Ag-NOR staining revealed that nucleolar organizer regions (NORs) are located on one pair of microchromosomes. In L.dilepis and L. anomalus the 4th chromosome pair is heteromorphic, and we suggest that it might be considered as the ZW sex chromosome pair. The optimization of available karyologicaldata on a molecular phylogenetic tree of the tribe Xenodontini shows that the diploid numbers 2n = 28, 30 and 34 represent putative synapomorphy for Erythrolamprus, Xenodon and Lygophis, respectively. Our results provide new insights which fill gaps in our knowledge on the cytology in the genus Lygophis and identified a possible diagnostic character for the genus.","palabras_clave":"Dipsadidae|karyosystematic|Lygophis|variability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4863,"idioma_id":4,"titulo":"Modified Nucleoside Triphosphates for In-vitro Selection Techniques","resumen":"A guanine-rich DNA oligonucleotide complexed with heminwas used to catalyze controlled oxygen transfer reactions to different sulfidesfor sulfoxide preparation in the presence of H2O2.Comparable activities were obtained when using fully modified L-DNA. Inaddition, oligonucleotide immobilization led to an active catalyst which couldbe successfully recovered and reused without loss of activity. ","palabras_clave":"modified nucleotides|SELEX|aptamers|functional oligonucleotides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4864,"idioma_id":4,"titulo":"Long-term effects of early life stress exposure: role of epigenetic mechanisms","resumen":"Stress is an adaptive response to demands of the environment and thus essentialfor survival. Exposure to stress during the first years of life has been shown to have profound effects on the growth and development of an adult individual. There are evidences demonstrating that stressful experiences during gestation or in early life can lead to enhanced susceptibility to mental disorders. Early-life stress triggers hypothalamic-pituitary-adrenocortical (HPA) axis activation and the associated neurochemical reactions following glucocorticoid release are accompanied by a rapid physiological response. An excessive response may affect the developing brain resulting in neurobehavioral and neurochemical changes later in life. This article reviews the data from experimental studies aimed to investigate hormonal, functional, molecular and epigenetic mechanisms involved in the stress response during early-life programming. We think these studies might prove useful for the identification of novel pharmacological targets for more effective treatments of mental disorders.","palabras_clave":"ESTRÉS|EPIGENÉTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4866,"idioma_id":4,"titulo":"The role of small rodents in the seed dispersal process: Microcavia australis consuming Prosopis flexuosa fruits","resumen":"In the Monte desert, Prosopis produces abundant food, unevenly in time and space, in the form of pods and seeds. Mammals use Prosopis propagules extensively, and they could be predators or dispersers depending on how they handle and where they leave the propagules.  The objectives of this study were: 1) to know how long M. australis  transported propagules; 2) to evaluate what hoarding pattern was used by M. australis (larder and scatterhoarding) in comparison with Sigmodontine rodents; and 3) to analyze in which condition were propagules left.","palabras_clave":"disperser|larderhoarding|predator|rodent"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4867,"idioma_id":4,"titulo":"Defining New Parameters for Green Engineering Design of Treatment Reactors","resumen":"This study proposes a green way to design Plug Flow Reactors (PFR) that use biodegradable polymer solutions, capable of contaminant retaining, for industrial wastewater treatment. Usually, to the design of a reactor, the reaction rate is determined by tests on a Continuous Stirred-Tank (CST), these generate toxic effluents and also increase the cost of the design. In this work, empirical expressions (called ?slip functions?), in terms of the average concentration of the contaminant, were developed through the study of the transport behaviour of CrVI into solutions of xanthan gum. ?In situ? XR&#956;F was selected as a no-invasive micro-technique to determine local concentrations. Slip functions were used with laboratory experiments planned in similar conditions using Plug Flow Reactors, to obtain useful dimensionless parameters for the industrial design.","palabras_clave":"WASTEWATER TREATMENTS|BIODEGRADABLE POLYMERS|HEAVY METALS CONTAMINANTS|POLYMER RETENTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4868,"idioma_id":4,"titulo":"An improvement of the sensitivity of GPS radio occultation data to detect gravity waves through observational and modeling factors","resumen":"There is a mid-latitude region to the East of the Andes Range in the Southern Hemisphere that exhibits ideal conditions for t hegeneration of gravity waves (GW) by topography mainly during winter. The configuration favors the generation of wavefronts that are parallel to the North?South direction. Global Positioning System (GPS) radio occultation (RO) retrievals from the COSMIC (Constellation Observing System for Meteorology, Ionosphere and Climate) mission exhibit in a large proportion of the soundingsan orientation which should be favorable to the detection of these wavefronts. We try to verify if this GW activity surplus on the East with respect to the West in the studied zone in winter emerges clearly in the GPS RO data between years 2007 and 2012. We argue that the orientation of the soundings but also the mathematical model selected to represent the GW energy distribution can affect the possibility of detecting the signatures of the waves. In particular, we explore a new interpretation of the GW energy distribution observed by GPS RO at the lowest values, as they stay below the precision limit of the technique. We suggest to replace that part of the measured distribution by an exponential curve that in general suits the trend of all the other observed energies. In following this alternative it is shown that the calculated mountain wave activity in the studied sector is now even more clearly larger in the East than in the West during winter. Finally, we consider that energy distributions observed with any measurement technique should in general not be considered as the solely contribution from waves, as also other variable phenomena may be adding to the final outcome.","palabras_clave":"GRAVITY WAVES|RADIO OCCULTATION|ENERGY DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4869,"idioma_id":4,"titulo":"ENSO related moisture and temperature anomalies over South America derived from GPS radio occultation profiles","resumen":"El Niño Southern Oscillation- (ENSO) related anomaly patterns of monthly specific humidity (q), precipitablewater content (PW) and temperature (T) over South America are analysed using the FORMOSAT-3\/COSMIC mission data(August 2006?December 2013). The Global Positioning System radio occultation (GPS RO) technique provides high verticalresolution profiles of atmospheric properties (refractivity, density, temperature and water vapour pressure) with a globalcoverage under any weather condition. During the ENSO warm phase (or El Niño), positive PW anomalies are found over theequatorial Pacific Ocean, southeastern Brazil, Bolivia, Paraguay and northern Argentina. Negative anomalies are found in thenorthern part of South America, but covering a smaller area. During the ENSO cold phase (or La Niña), positive anomaliesare found in the northern part of the continent whereas negative anomalies are found over the Amazon Basin region. A Morletcontinuous wavelet transform analysis is applied to deseasonalized PW anomaly time series. Over the areas showing PWanomalies related to ENSO, main modes of oscillation between 35 and 38 months are found, similar to the main oscillationmode of the Oceanic Niño index. Consistent with previous studies, PW anomalies in almost all these areas show a generalbehaviour towards opposite signals during the opposite ENSO phases. q patterns show a linear (in phase with Oceanic NiñoIndex) response to ENSO along the equator below 8 km height. The Andes mountains separate positive (west) from negative(east) anomalies during El Niño (EN) events (reversing during LN). The higher q positive anomalies are found over the equatorialPacific Ocean and over 60&#8728;?50&#8728;W, 35&#8728;?20&#8728;S during EN months. T patterns show positive anomalies in the whole tropicaltroposphere during EN months, with higher values over the equatorial Pacific, west of the Andes between 7 and 15 km height.","palabras_clave":"ENSO|GPS RO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4870,"idioma_id":5,"titulo":"Una propuesta integradora de Mantenimiento Correctivo aplicada al Diseño Web Adaptativo y Accesibilidad Web","resumen":"El incremento de las TIC ha promovido el desarrollo de herramientas informáticas libres y gratuitas, como los Content Management System o Sistemas de Gestión de Contenidos que facilitan la generación eficaz de contenidos digitales. Éstos deben ser evaluados previos a su utilización, a los efectos de producir un producto accesible por todos los usuarios. Se presenta una experiencia de Mantenimiento Correctivo del software aplicada a la Accesibilidad Web y el Diseño Web Adaptativo. La incorporación de estas pautas en el desarrollo de software, permiten impulsar la generación de productos tecnológicos de calidad y fomentan la inclusión digital de los individuos independientemente de sus capacidades","palabras_clave":"MANTENIMIENTO CORRECTIVO|ACCESIBILIDAD WEB|DISEÑO WEB ADAPTATIVO|SISTEMAS GESTORES DE CONTENIDOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4871,"idioma_id":5,"titulo":"Abordaje de tecnología GIS en trabajos de graduación de la Licenciatura en Sistemas de Información","resumen":"La asignatura Trabajo Final de Aplicación (TFA) es el espacio curricular donde se generan las tesinas de la carrera Licenciatura en Sistemas de Información. En el trabajo se sintetizan las producciones tecnológicas diseñadas y desarrolladas integrando los Sistemas de Información Geográfica o GIS para la resolución de problemas de gestión y despliegue de información aplicados a dominios del ámbito local y regional. El objetivo de la ponencia es contribuir desde la Universidad hacia su contexto en un énfasis de promover la vinculación de los actores con responsabilidad social y aportando a la sociedad del conocimiento, en este caso haciendo uso de los GIS","palabras_clave":"TECNOLOGÍAS DE LA INFORMACIÓN Y COMUNICACIÓN|SISTEMAS DE INFORMACIÓN GEOGRÁFICA|TRABAJOS DE GRADUACIÓN|EDUCACIÓN SUPERIOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4872,"idioma_id":4,"titulo":"Identification and characterization of antioxidant peptides obtained by gastrointestinal digestion of amaranth proteins","resumen":"The objective of the present work was to separate and identify antioxidant peptides from a simulated gastrointestinal digest (Id) from Amaranthus mantegazzianus proteins (I), which has previously demonstrated to have this activity. I and Id were separated by preparative RP-HPLC. Fractions were evaluated by the ORAC method and the more active ones were analyzed by LC-MS\/MS. Each fraction presented diverse peptides from different proteins, most of them from the 11S globulin. After grouping of these last ones according to their overlapping sequences, and based on previous information about structure-activity relationships, some sequences (10) were synthesized, evaluating the antioxidant activity of them. Four peptides presented interesting activity: AWEEREQGSR > YLAGKPQQEH ~ IYIEQGNGITGM ~ TEVWDSNEQ. They exhibited some of the structural characteristics previously informed for this activity, presenting all of them at least a bulky aromatic residue. All belonged to little structured, internal or exposed regions of the acid subunit of the 11S globulin.","palabras_clave":"amaranth proteins|peptides|gastrointestinal digestion|antioxidant activity|structure-activity relationship"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4873,"idioma_id":4,"titulo":"The Vertebral Column of the Hourglass Dolphin (Lagenorhynchus cruciger, Quoy and Gaimard, 1824), with Notes on Its Functional Properties in Relation to Its Habitat","resumen":"Fast-swimming dolphins have a relatively stable morphological configuration, explained partially by their vertebral morphology. The hourglass dolphin (Lagenorhynchus cruciger), an oceanic species, is one of the least known species of small odontocetes. The aim of this paper is to describe the osteology of the vertebral column of this species, relating the main morphological characteristics to swimming performance in an oceanic habitat. We also present five new records, with meristics and measurements of the postcranial skeleton in conjunction with an exhaustive characterization of each functional region of the vertebral column through morphometric and graphi- cal interpretations. In this species, the stability of the mid-torso is reinforced by the lumbarization and high number of vertebrae. While the morpho- logical process indicates a mechanical advantage for the swimming muscles, the mid-torso appears to act as an ?oscillatory beam? to store potential energy, working as an elastic spring. Tail displacements are mainly produced by the flexion of the peduncle, which undulates from a stable mid- region. As suggested for other fast-swimming dolphins, morphological adaptations in the hour- glass dolphin fit a typical pelagic mode of life, with a highly stable column that minimizes energy consumption, increasing efficiency for prolonged swimming.","palabras_clave":"Hourglass dolphin|Lagenorhynchus cruciger|Vertebral column|Functional morphology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4874,"idioma_id":5,"titulo":"Parasitosis intestinales y ambiente: un estudio preliminar en la población de horticultores del Parque Pereyra Iraola (Buenos Aires, Argentina)","resumen":"Introducción: En el Parque Pereyra Iraola viven familias de pequeños productores hortícolas que abastecen al mercado regional. El presente trabajo aborda un estudio parasitológico integral, con el fin de evaluar las condiciones sanitarias y su impacto en la salud humana. Materiales y métodos: Se analizaron muestras seriadas de heces humanas y escobillado anal (n=80), heces caninas (n=8), de animales de cría (n=12), y muestras de hortalizas (n=21). Se aplicaron técnicas de concentración por flotación y sedimentación. Mediante encuestas semiestructuradas se relevaron variables socio-ambientales. Resultados: El 84% de la población humana resultó parasitada. Las mayores prevalencias correspondieron a Blastocystis sp. (41,2%), Enterobius vermicularis (37,5%) y Entamoeba coli (33,7%). Se hallaron asociaciones significativas entre especies parásitas. En perros la especie más prevalente fue Trichuris vulpis (37,5%), seguida de Ancylostoma caninum, Uncinaria stenocephala y Toxocara canis (25%). En animales de cría se hallaron Balantidium coli, Trichuris suis, Capillaria sp., Heterakis sp., Trichostrongylus sp, entre otros. En las hortalizas se encontraron quistes de amebas, ooquistes de coccidios, huevos de estrongilídeos y larvas de nematodes de vida libre. La presencia de G. lamblia en la población humana se asoció significativamente con trabajo inestable de la madre, falta de educación paterna y tenencia de huerta.Conclusiones: Los resultados observados en la población y las especies de importancia zoonótica en animales y hortalizas, sugieren profundizar estos estudios y desarrollar medidas de control y saneamiento ambiental, que mejoren la calidad de vida de los horticultores y su actividad productiva.","palabras_clave":"PARASITOSIS INTESTINALES|HORTICULTORES|ANIMALES|HORTALIZAS|PARQUE PEREYRA IRAOLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4875,"idioma_id":4,"titulo":"Trichinella spiralis infection and transplacental passage in human pregnancy","resumen":"Abstract: Infection by Trichinella spp. during pregnancy still arisesmany questions. Worldwide an important number of trichinellosis outbreakshave occurred over time where pregnant women were or might have beeninvolved.Parasitological and immunoserological parameters, clinical background andtransplacental passage of Trichinella spiralis of six pregnant women,from different outbreaks, were studied.Serum samples were collected from patients and their children: atpregnancy, at delivery, newborn and\/or infant (&#8805; 1 year-old). Totalimmunoglobulins (Igs) and isotypes against excretion-secretion productsfrom muscle larvae (ESP-ML) and newborn larvae (NBL) of T. spiralis weredetected by immunoserological techniques. Sera helminthocytotoxicactivity against NBL was evaluated by antibodies dependent cellcytotoxicity assay.Patients presented similar clinical symptoms. Mother´s sera showeddifferences in the presence and titres of Igs, IgE, IgG1 and IgG4 againstNBL surface. All of them showed helminthocytotoxic activity against NBL,four in progesterone dependent manner and the other two by antibodies.Igs anti ESP-ML were present in mother´s sera, with differences in theisotypes detected. Anti-ESP-ML IgE, IgA and\/or IgM in newborn sera wereobserved. Three out of four infants showed specific Igs; one even at 8years-old.The distinct immune response between mothers at pregnancy evidenced bysera helminthocytotoxic activity and immunoserological parameters may beassociated with a different parasite burden exposition. Specific Igsobserved in newborns would be a suggestion of transplacental passage ofthis Igs and\/or their production by the fetus. Specific Igs found ininfants sera suggests that newborn larvae transplacental passage ispossible.","palabras_clave":"TRICHINELLA SPIRALIS|HUMAN PREGNANCY|INMUNOLOGICAL PARAMETERS|HELMINTHOCYTOTOXICITY|TRANSPLACENTAL PASSAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4876,"idioma_id":4,"titulo":"Partial isometries and pseudoinverses in semi-Hilbertian spaces","resumen":"In this article the concepts of partial isometries, normal partial isometries and generalized projections in the context of operators defined on a semi-Hilbertian space are developed. In particular, we analyze the relationship between these operators and different notions of pseudoinverses in semi-Hilbertian spaces. Finally, we apply the results obtained to describe the set of weighted projections into closed subspaces.","palabras_clave":"SEMI-HILBERTIAN SPACES|PARTIAL ISOMETRIES|PSEUDOINVERSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4877,"idioma_id":4,"titulo":"Implicitization of rational hypersurfaces via linear syzygies: a practical overview","resumen":"We unveil in concrete terms the general machinery of the syzygy-based algorithms for the implicitization of rational surfaces in terms of the monomials in the polynomials defining the parametrization, following and expanding our joint article with M. Dohm. These algebraic techniques, based on the theory of approximation complexes due to J. Herzog, A. Simis and W. Vasconcelos, were introduced for the implicitization problem by J.-P. Jouanolou, L.Busé, and M. Chardin. Their work was inspired by the practical method of moving curves, proposed by T. Sederberg and F. Chen, translated into the language of syzygies by D. Cox. Our aim is to express the theoretical results and resulting algorithms into very concrete terms, avoiding the use of the advanced homological commutative algebraic tools which are needed for their proofs.","palabras_clave":"implicitación|superficies racionales|polítopos enteros|algoritmo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4878,"idioma_id":4,"titulo":"Ligand mediated galectin-1 endocytosis prevents intraneural H2O2 production promoting F-actin dynamics reactivation and axonal re-growth.","resumen":"AbstractAxonal growth cone collapse following spinal cord injury (SCI) is promoted by semaphorin3A (Sema3A) signaling via PlexinA4 surface receptor. This interaction triggers intracellular signaling events leading to increased hydrogen peroxide levels which in turn promote filamentous actin (F-actin) destabilization and subsequent inhibition of axonal re-growth. In the current study, we demonstrated that treatment with galectin-1 (Gal-1), in its dimeric form, promotes a decrease in hydrogen peroxide (H2O2) levels and F-actin repolimerization in the growth cone and in the filopodium of neuron surfaces. This effect was dependent on the carbohydrate recognition activity of Gal-1, as it was prevented using a Gal-1 mutant lacking carbohydrate-binding activity. Furthermore, Gal-1 promoted its own active ligand-mediated endocytosis together with the PlexinA4 receptor, through mechanisms involving complex branched N-glycans. In summary, our results suggest that Gal-1, mainly in its dimeric form, promotes re-activation of actin cytoskeleton dynamics via internalization of the PlexinA4\/Gal-1 complex. This mechanism could explain, at least in part, critical events in axonal regeneration including the full axonal re-growth process, de novo formation of synapse clustering, axonal re-myelination and functional recovery of coordinated locomotor activities in an in vivo acute and chronic SCI model.SIGNIFICANCE STATEMENT: Axonal regeneration is a response of injured nerve cells critical for nerve repair in human spinal cord injury. Understanding the molecular mechanisms controlling nerve repair by Galectin-1, may be critical for therapeutic intervention. Our results show that Galectin-1; in its dimeric form, interferes with hydrogen peroxide production triggered by Semaphorin3A. The high levels of this reactive oxygen species (ROS) seem to be the main factor preventing axonal regeneration due to promotion of actin depolymerization at the axonal growth cone. Thus, Galectin-1 administration emerges as a novel therapeutic modality for promoting nerve repair and preventing axonal loss.","palabras_clave":"Galectin-1|endocytosis|intrneural"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4879,"idioma_id":4,"titulo":"A  Bayesian method  foranalysing relaxation spectra","resumen":"The knowledge of electrical and mechanical properties of material, relies on a precise analysis of the relaxation spectra. We explore the ability of a Bayesian method to achieve an accurate estimation of spectral parameters. We implemented a parallel-tempering Markov-chain Monte Carlo algorithm and used it to fit simulated and measured spectra. An exhaustive testing of the code shows that it presents an extremely good performance, accurately fitting complex spectra under strong noise and overlapping components.Weconclude that this technique is quite suitable for relaxation spectra analysis, complementing classical methods.","palabras_clave":"Dielectric spectroscopy|Mechanical spectroscopy| Spectral analysis|Bayesian methods |Monte Carlo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4880,"idioma_id":4,"titulo":"Hepatic and renal metallothionein concentrations in Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, South Atlantic Ocean","resumen":"The Commerson´s dolphin is the most common endemic odontocete of subantarctic waters of Tierra del Fuego, Argentina incidentally caught in fishing nets. The species is classified as ?Data Deficient? by the IUCN. Metallothioneins (MTs) are considered as suitable biomarkers for health and environmental monitoring. The aims of the study were to assess MT concentrations in the liver and kidney of bycaught specimens. Moreover, correlations with Zn, Se, Cd, Ag and Hg, and the molar ratios of MT:metals were estimated to evaluate if there is an indication of their respective protective role against metal toxicity in tissues. Hepatic and renal MT concentrations were similar, ranging from 11.6 to 29.1 nmol·g&#8722; 1 WW, and Kidney\/Liver ratios ranging from 0.73 to 1.93 corresponded to normal ranges. Results suggest that MTs are related to physiological ranges for the species. This information constitutes the first MT report on Commerson´s dolphins and possibly considered as baseline for species´ conservation.","palabras_clave":"Metallothioneins|Trace elements|Subantarctic dolphins|Southwestern South Atlantic Ocean"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4881,"idioma_id":4,"titulo":"Layered double hydroxides-indomethacin nanohybrids: intercalation, pH influence, stability and release properties","resumen":"In this work, three pH values (8, 9 and 10) were studied for the insertion of Indomethacin (Indo) molecules into Layered Double Hydroxides (LDHs). The obtained results showed that the LDH materials have been a good storage for the drug. LDHs provide thermal stability with an increase in the thermal decomposition of the drug around 100°C more. Indo into LDHs exhibited higher photostability to UV light irradiation. In vitro drug release experiments in a phosphate buffer solution (pH = 7.4) have been carried out. The loading amount of intercalated Indo was increased to 66 % at pH 8, and showed a profile of sustained release of 97 % in 8h. The release profiles were fitted by mathematical models, which describe various kinetic models that served to investigate the drug release mechanism, being the Bhaskar kinetics model the most appropriate. The results showed that the nanohybrids can be used as an effective drug delivery system.","palabras_clave":"LAYERED DOUBLE HYDROXIDE|STABILITY PROPERTIES|INDOMETHACIN|DRUG DELIVERY SYSTEM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4882,"idioma_id":4,"titulo":"Photodynamic therapy of HeLa cell cultures by using LED or laser sources","resumen":"The photodynamic therapy (PDT) on HeLa cell cultures was performed utilizing a 637 nm LED lamp with 1.06W power and m-tetrahydroxyphenyl chlorin (m-THPC) as photosensitizer and compared to a laser source emitting at 654 nm with the same power. Intracellular placement of the photosensitizer and the effect of its concentration (CP), its absorption time (TA) and the illuminationtime (TI) were evaluated. It was observed that for CP > 40 &#956;g\/ml and TA = 24 h, m-THPC had toxicity on cells in culture, even in the absence of illumination. For the other tested concentrations, the cells remained viable if not subjected to illumination doses. No effect on cells was observed for CP < 0.05 &#956;g\/ml, TA = 48 h and TI = 10 min and they continued proliferating. For drug concentrations higher than 0.05 &#956;g\/ml, further deterioration is observed with increasing TA and TI. We evaluated the viability of the cells, before and after the treatment, and by supravital dyes, and phase contrast and fluorescence microscopies, evidence of different types of cell death was obtained. Tetrazolium dye assays after PDT during different times yielded similar results for the 637 nm LED lamp with an illuminance three times greater than that of the 654 nm laser source. Results demonstrate the feasibility of using a LED lamp as alternative to laser source. Here the main characteristic is not the light coherence but achieving a certain light fluence of the appropriate wavelength on cell cultures. We conclude that the efficacy was achieved satisfactorily and is essential for convenience, accessibility and safety.","palabras_clave":"PHOTODYNAMIC THERAPY|HELA CULTURES|LED vs LASER|M-THPC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4883,"idioma_id":4,"titulo":"Impact of thermal annealing on wettability and antifouling characteristics of alginate poly-l-lysine polyelectrolyte multilayer films","resumen":"Polyelectrolyte multilayers (PEMs) of poly- l-lysine (PLL) and alginic acid sodium salt (Alg) are fabricated applying the layer by layer technique and annealed at a constant temperature; 37, 50 and 80 °C, for 72 h. Atomic force microscopy reveals changes in the topography of the PEM, which is changing from a fibrillar to a smooth surface. Advancing contact angle in water varies from 36° before annealing to 93°, 77° and 95° after annealing at 37, 50 and 80 °C, respectively. Surface energy changes after annealing were calculated from contact angle measurements performed with organic solvents. Quartz crystal microbalance with dissipation, contact angle and fluorescence spectroscopy measurements show a significant decrease in the adsorption of the bovine serum albumin protein to the PEMs after annealing. Changes in the physical properties of the PEMs are interpreted as a result of the reorganization of the polyelectrolytes in the PEMs from a layered structure into complexes where the interaction of polycations and polyanions is enhanced. This work proposes a simple method to endow bio-PEMs with antifouling characteristics and tune their wettability.","palabras_clave":"POLYELECTROLYTE MULTILAYERS|THERMAL ANNEALING|BIOPOLYMERS|WETTABILITY|ANTIFOULING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4885,"idioma_id":4,"titulo":"Biodegradability and plasticizing effect of yerba mate extract on cassava starch edible films","resumen":"Biodegradable and edible cassava starch-glycerol based films with different concentrations of yerba mate extract (0, 5 and 20 wt.%) were prepared by casting. The plasticizing effect of yerba mate extract when it was incorporated into the matrix as an antioxidant was investigated. Thermal degradation and biodegradability of the obtained biofilms were also studied. Thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), attenuated total reflectance Fourier transform infrared spectroscopy (ATR\/FTIR), X-ray diffraction analysis (XRD), water absorbance, stability in different solutions and biodegradability studies were performed. The clear correlation among the results obtained from the different analysis confirmed the plasticizing effect of yerba mate extract on the starch-glycerol matrix. Also, the extract led to a decrease in the degradation time of the films in soil ensuring their complete biodegradability before two weeks and to films stability in acidic and alkaline media. The plasticizing effect of yerba mate extract makes it an attractive additive for starch films which will be used as packaging or coating; and its contribution to an earlier biodegradability will contribute to waste reduction.","palabras_clave":"Biodegradable edible films|Cassava starch|Yerba mate extract|Plasticizing effect"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4886,"idioma_id":4,"titulo":"A Lyapunov type inequality for indefinite weights and eigenvalue homogenization","resumen":"In this paper we prove a Lyapunov type inequality for quasilinear problems with indefinite weights. We show thatthe first eigenvalue is bounded below in terms of the integral of the weight, instead of the integral of its positive part. We apply this inequality to some eigenvalue homogenization problems with indefinite weights.","palabras_clave":"eigenvalues|homogenization|quasilinear equations|Lyapunov inequalities"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4887,"idioma_id":4,"titulo":"Eigenvalue homogenization for quasilinear elliptic equations with different boundary conditions","resumen":"We study the rate of convergence for (variational) eigenvalues of several non-linear problems involving oscillating weights and subject to different kinds of boundary conditions in bounded domains.","palabras_clave":"eigenvalues|homogenization|order of convergence|Steklov"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4888,"idioma_id":4,"titulo":"ZOOPLANKTON DIVERSITY OF A PROTECTED AND VULNERABLE WETLAND SYSTEM IN SOUTHERN SOUTH AMERICA (LLANCANELO AREA, ARGENTINA)","resumen":"Inarid regions, climatic conditions exert a great control on the aquatic systemspresent, but recent changes in climate have produced an enhanced salinizationof the aquatic environments located there. Consequently, a major reduction inbiodiversity would be expected in those wetlands that were originally freshwater. Salinity is a principal cause of reduced biodiversity particularly inzooplankton because few of those species can adapt to the salt pressure ofsaline environments. Therefore, the aim of this study was to gain essentialinformation on the diversity of aquatic invertebrates in Llancanelo basin byfocussing the analysis on the zooplankton community and exploring seasonal andspatial differences in the zooplankton assemblages of this vulnerable wetlandsystem within an arid region of Argentina. Seasonal samples were taken at 9sites in the basin (a shallow lake, 4 springs, streams, and the MalargüeRiver). A total of 45 species were identified. The zooplankton abundance in thelake displayed a clear seasonal contrast and was higher than that recorded inthe springs and lotic environments.Boeckella poopoensis, Fabrea salina, and Brachionus plicatilis predominated in the lake, indicating theirhalophilia. The presence of the crustaceans Alonasp., Macrocyclops albidus, and Paracyclops fimbriatus was restricted tothe springs; whereas Notholca labisand Notholca squamula were found onlyin running water. The zooplankton species richness in the Llancanelo area islow because of both the salt content in the lake and the irregularity offreshwater entry in all locations during the annual cycle.","palabras_clave":"ZOOPLANKTON|STRUCTURE |WETLAND SYSTEM |SALINIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4889,"idioma_id":4,"titulo":"Adsorption of Nisin on Montmorillonite:  A Concentration Strategy","resumen":"The adsorption of a commercial form of nisin, Nisaplin® (Npl) (Danisco), on montmorillonite (Mt), a natural inexpensive clay mineral accepted as food additive, was assayed. The intention was to develop food grade materials capable of releasing the peptide and avoiding negative interactions that affect nisin antimicrobial activity in food environments. A set of different Mt-Npl relation samples was prepared. The antimicrobial activity and the Total Organic Carbon\/Total Nitrogen (TOC\/TN) content measured in nisin solutions after contact with the clay decreased as the amount of solid increased; thus, indicating the adsorption of the peptide on the support. Also, the Mt-Npl solids presented inhibitory activity against Enterococcus faecium C1. Additionally, Fourier Transform Infrared Attenuated Total Reflectance spectroscopy (FTIR-ATR) analysis of the Mt-Npl lyophilised systems showed that nisin was preferably adsorbed on Mt among Nisaplin® ingredients, suggesting a unique concentration and immobilization method for this antimicrobial peptide that could be applied in food preservation.","palabras_clave":"NISIN|MONTMORILLONITE|FOOD BIOPRESERVATIVES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4890,"idioma_id":4,"titulo":"Climatic thresholds for concentrations of minerals and heavy metals in Argentinean soybeans","resumen":"Minerals affect the nutritional, rheological, and safe features of food products. Soybeans represent a good source of minerals. The objective of this study was to evaluate the effect of the environment on the variability of mineral elements in Argentinean soybeans in field experiments. Climatic variables (maximum, mean, and minimum air temperature, solar radiation, precipitation, and potential evapotranspiration) were recorded daily during the seed filling period; soil properties were also reported. Minerals in soybeans were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Selenium (Se) was determined by hydride generation coupled to atomic absorption spectrophotometer. Molybdenum (Mo) and Se were significantly increased (over 100%) in soybeans grown at higher soil pH with high available Mo. Air temperature was the climatic variable that best predicted changes in the soybean seed mineral composition. Optimum weather conditions (OWC) were defined by thresholds of the climatic variables by regression trees for desirable mineral composition. A maximum and minimum daily air temperatures during the seed filling period (30.1 °C and 17.1 °C, respectively) were the OWC for maximizing calcium (Ca), magnesium (Mg), and manganese (Mn) contents. A maximum daily air temperature over 28.0 °C resulted in higher iron (Fe) and cobalt (Co) (p<0.001). Maximum zinc (Zn) content was observed when solar radiation exceeded 18.1 MJ m-2 during seed filling (p<0.001). Results from this study showed variation in the mineral composition of soybeans. Environmental features during the seed filling period should be considered when desired mineral composition is expected in soybean according to the end uses.","palabras_clave":"SOYBEANS|MINERAL|ENVIROMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4891,"idioma_id":4,"titulo":"A stem falconid bird from the Lower Eocene of Antarctica and the early southern radiation of the falcons","resumen":"Antarctoboenus carlinii gen. et sp. nov. is a large-sized falconiform bird coming from sediments of La Meseta Formation (Lower Eocene) at Seymour (Marambio) Island, West Antarctica. The holotypical tarsometatarsus was originally assigned to Falconidae and its affinities were pointed out with Polyborinae. Detailed osteological and comparative analyses of the Antarctic specimen allowed recognizing the new taxon as a member of the stem group Falconidae, i.e., it is supposed to belong to the early radiation of the falconiform lineage. Antarctoboenus carlinii gen. et sp. nov. distinguishes from members of the crown group Falconidae by having a very shallow sulcus extensorius, a large foramen vasculare distale, an undistinguishable tendinal attachment of the m. adductor digiti II, and short trochleae metatarsi II et IV, among its main diagnostic characters. Purported phylogenetic relationships between A. carlinii and Polyborinae are based on plesiomorphic characters retained in the tarsometatarsus of the latter clade. Our conclusions reinforce the hypothesis about the Neotropical or Austral origin of Falconidae supported by previous molecular phylogenies.","palabras_clave":"FALCONIFORMES|STEM LINEAGE|PALEOGENE|ANTARCTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4892,"idioma_id":4,"titulo":"Variation for Health-Enhancing  Compounds and Traits in Onion  (Allium cepa L.) Germplasm","resumen":"Consumption of onion has been associated with reduced incidence of chronic diseases. Phenolic, organosulfur and carbohydrate compounds present are largely responsible for these effects. This study examined compositional variation for health-enhancing compounds in a genetically diverse collection of onion cultivars. Total antioxidant activity and aroma profiles were characterized. Significant variation in bulb concentration for total and individual phenolic compounds, thiosulfinates, carbohydrates, and total and soluble solids was found. The range of variation was particularly large (>50-fold difference between the cultivars with the highest and lowest content) for fructo-oligosaccharides (FOS) and the polyphenols quercetin, epigallocatechin gallate and epicatechin gallate. Amino acid profiles varied significantly as well with substantial variation (~10 fold) observed in both total and essential amino acids. Total antioxidant activity was positively correlated with polyphenols content, and quercetin in particular (r = 0.83), suggesting a major contribution from phenolic compounds to onion antioxidant properties. Significant positive correlation was also found between solids and thiosulfinates content (r = 0.74) and between solids and FOS (r = 0.81), suggesting a dilution\/concentration effect for organosulfur compounds and FOS in onion bulbs. The present study revealed broad variation for health-enhancing compounds content in onion germplasm, which can be exploited in breeding programs aiming at increasing onion nutraceutical value.","palabras_clave":"ONION| POLYPHENOLS|CARBOHYDRATES|AMINO ACIDS|AROMA PROFILES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4893,"idioma_id":5,"titulo":"La transformación de las funciones de la asociatividad en una cooperativa de cartoneros: de la representación al trabajo productivo","resumen":"En las últimas décadas, los recuperadores urbanos de residuos y los procesos de formación de cooperativas han sido objeto de estudio de numerosos análisis. Sin embargo, no son muchos los trabajos que indaguen en la heterogeneidad de las cooperativas de recuperadores de Buenos Aires y la especificidad de cada proceso de formación. Como un modo de comenzar a plantear esta cuestión, nos proponemos indagar en el proceso de conformación de una cooperativa en el período 2001-2015, identificando sus particularidades y dando cuenta de los efectos que ha tenido en los cartoneros la participación en esta nueva forma de asociatividad. Nos interesa, además, analizar las transformaciones subjetivas de los propios actores en relación con los procesos asociativos. La pregunta de la que partimos será para qué se asociaron, para qué formaron cooperativas.","palabras_clave":"RECUPERADORES URBANOS|COOPERATIVAS|TRABAJO|POLITICAS PUBLICAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4894,"idioma_id":4,"titulo":"DNA barcoding of Phymaturus lizards reveals conflicts in species delimitation within the patagonicus clade","resumen":"Under the DNA Barcode initiative, we used the mitochondrial locus cytochrome c oxidase I (COI) to test if this molecular marker would reliably distinguish among lizard species of the patagonicus clade of Phymaturus. Using 18 described species and two populations of unidenfied species, we calculated intra- and inter-population genetic distances for all operational taxonomic units (OTU) and performed phylogenetic reconstructions using maximum parsimony and maximum likelihood. We identified different species that share the same barcode index number (BIN). Only 12 of the 18 previously described species were recovered, and one candidate species from the new population. By comparing our results with published morphological and molecular phylogenies as well as previous debates, we propose possible explanations for this. In some cases (such as the group with the same BIN formed by P. spurcus, P. spectabilis, P. excelsus, and P. agilis), where the identity of the species was previously debated by other authors, we suggest that the low genetic distances could be attributable to the presence of one species with high polymorphism. On the other hand, in geographically-isolated species such as the group formed by P. payuniae and P. nevadoi, the group formed by P. somuncurensis and P. ceii, and the group formed by P. indistinctus and P. videlai, the topology of the phylogenetic trees indicates that the low genetic distances (also found by other authors analyzing cytochrome b) could be attributable to shared ancestral polymorphism resulting from incomplete lineage sorting.","palabras_clave":"COI|LIOLAEMIDAE|PATAGONIA|POLYMORPHISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4895,"idioma_id":4,"titulo":"Auxin effects on Pb phytoextraction from polluted soils by Tegetes minuta L. and Bidens pilosa L.: Extractive power of their root exudates","resumen":"The principal impediment for Pb uptake by plants is the Casparian strip in roots. It prevents metals reaching the xylem, thereby hampering translocation to the aerial organs. In the root apices, young root cells have thin cell walls and the Casparian strip is not completely developed, which could facilitate Pb uptake by roots at these vulnerable points. However, as the phytotoxic effects of Pb reduce root growth and enhance suberization, entry of Pb into the plant is avoided. We propose that the application of root growth promotors could be an important complement in the phytoextraction of Pb from polluted soils, due to their effects on produced biomass, Pb toxicity, and root exudate production.A greenhouse experiment was carried on to evaluate the auxin application effect on the Pb uptake of Bidens pilosa and Tagetes minuta. These species were sensitive to auxins, but the phytotoxic effect of Pb was not reversed by this treatment. Root exudates capable of extracting Pb were produced only when the species were grown in highly polluted soils, indicating a behavioral response to Pb exposure which is desirable for phytoremediation.","palabras_clave":"Lead soil pollution|Bidens pilosa|Tagetes minuta|Phytohormones|Root exudates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4896,"idioma_id":4,"titulo":"Soil variables that determine lead accumulation in Bidens pilosa L. and Tagetes minuta L. growing in polluted soils","resumen":"Toxic metal soil pollution is one of the most serious environmental problems, with phytoextraction being an ecological and inexpensive potential solution to recuperate soil functionality. This emergent technology has produced good results for many elements, but phytoextraction has not been as successful for lead as for other pollutants. This research aimed to evaluate several factors that could be determinant on Pb uptake by the accumulator plants Bidens pilosa and Tagetes minuta. Topsoils (bulk and ryzospheric) and plants were collected around a former Pb smelter, and sequential extractions of Pb, Cu and Zn, organic matter content, electrical conductivity, pH, and texture were measured in soils. Also, bacterial diversity in soil was determined by the denaturing gradient gel electrophoresis (DGGE) technique. The Pb, Cu, and Zn concentrations in soil extractions and plants (leaves, stem, and root) were quantified by T-XRF in Synchrotron, Brazil. Our results demonstrated that Pb uptake from polluted soils and translocation to aerial tissues by two Asteraceae species are related not only to the traditional soil parameters, but even more importantly to the Zn and Cu concentrations in plant and soils. In addition, the soil bacterial biodiversity also affects the Pb uptake by plants. In this study, we propose the total translocation factor (TTF) to evaluate the phytoextraction efficiency, which is given by the ratio of total extraction of Pb in aerial organs and roots, instead of using the Pb concentrations alone.","palabras_clave":"Soil pollution|Lead|Copper|Zinc| Accumulation|Translocation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4897,"idioma_id":4,"titulo":"Ischemic postconditioning confers cardioprotection and prevents reduction of Trx-1 in young mice, but not in middle-aged and old mice","resumen":"ABSTRACT Thioredoxin-1 (Trx-1) is part of an antioxidant system that maintains the cell redox homeostasis but it is unknown their role on ischemic postconditioning (PostC). The aim of this work was to determine whether Trx-1 participates in the cardioprotective mechanism of PostC in young, middle-aged, and old mice. Male FVB young (Y: 3 months-old), middle-aged (MA: 12 months-old) and old (O: 20 months-old) mice were used. Langendorff-perfused hearts were subjected to 30 minutes of ischemia and 120 minutes of reperfusion (I\/R group). After ischemia, we performed 6 cycles of R\/I (10 seconds each) followed by 120 minutes of reperfusion (PostC group). We measured the infarct size (triphenyltetrazolium); Trx-1, total and phosphorylated Akt and GSK3&#946; expression (western blot); and the GSH\/GSSG ratio (HPLC). PostC reduced the infarct size in young mice (I\/R-Y: 52.3±2.4 vs. PostC-Y: 40.0±1.9 p<0.05), but this protection was abolished in the middle-aged and old mice groups. Trx-1 expression decreased after I\/R, and the PostC prevented the protein degradation in young animals (I\/R-Y: 1.05±0.1 vs. PostC-Y: 0.52±.0.07, p<0.05). These changes were accompanied by an improvement in the GSH\/GSSG ratio (I\/R-Y: 1.25±0.30 vs. PostC-Y: 7.10±2.10, p<0.05). However, no changes were observed in the middle-aged and old groups. Cytosolic Akt and GSK3&#946; phosphorylation increased in the PostC compared with the I\/R group only in young animals. Our results suggest that PostC prevents Trx-1 degradation, decreasing oxidative stress and allowing the activation of Akt and GSK3&#946; to exert its cardioprotective effect. This protection mechanism is not activated in middle-aged and old animals.","palabras_clave":"Ischemic|cardioprotection|reduction|Trx-1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4898,"idioma_id":4,"titulo":"The role of mitochondria in inflammatory syndromes","resumen":"Several authors have addressed the importance of mitochondrial function in inflammatory syndromes,as it may play a role in the genesis of tissue injury. Sepsis and exposition to environmental particles are examples of inflammatory conditions. Sepsis occurs with an exacerbated inflammatory response that damagestissue mitochondria and impairs bioenergetic processes. One of the current hypotheses for the molecular mechanisms underlying the complex condition of sepsis is that enhanced NO production and oxidative stress lead to mitochondrial dysfunction, bioenergetic derangement and organ failure. The mechanism of particulate matter-health effects are believed to involve inflammation and oxidative stress. Components in particles that elicit inflammation have been poorly investigated, although recent research points out to the contribution of compositional elements and particle size. Oxygen metabolism and mitochondrial function appear to be important areas of study in inflammatory conditions for clarifying molecular mechanisms involved.","palabras_clave":"mitochondrial function|bioenergetics|endotoxemia|ambient air particles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4899,"idioma_id":4,"titulo":"Acute exposure to air pollution particulate matter aggravates experimental myocardial infarction in mice by potentiating cytokine secretion from lung macrophages","resumen":"Abstract Clinical, but not experimental evidence hassuggested that air pollution particulate matter (PM)aggravates myocardial infarction (MI). Here, we aimed todescribe mechanisms and consequences of PM exposure inan experimental model of MI. C57BL\/6J mice were challengedwith a PM surrogate (Residual Oil Fly Ash, ROFA)by intranasal installation before MI was induced by permanentligation of the left anterior descending coronaryartery. Histological analysis of the myocardium 7 daysafter MI demonstrated an increase in infarct area andenhanced inflammatory cell recruitment in ROFA-exposedmice. Mechanistically, ROFA exposure increased thelevels of the circulating pro-inflammatory cytokines TNFa,IL-6, and MCP-1, activated myeloid and endothelialcells, and enhanced leukocyte recruitment to the peritonealcavity and the vascular endothelium. Notably, these effectson endothelial cells and circulating leukocytes could bereversed by neutralizing anti-TNF-a treatment. We identifiedalveolar macrophages as the primary source of elevatedcytokine production after PM exposure. Accordingly,in vivo depletion of alveolar macrophages by intranasalclodronate attenuated inflammation and cell recruitment toinfarcted tissue of ROFA-exposed mice. Taken together,our data demonstrate that exposure to environmental PMinduces the release of inflammatory cytokines from alveolarmacrophages which directly worsens the course of MIin mice. These findings uncover a novel link between airpollution PM exposure and inflammatory pathways, highlightingthe importance of environmental factors in cardiovasculardisease.","palabras_clave":"Myocardial infarction  | Particulate matter  |Inflammation |Monocyte"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4901,"idioma_id":4,"titulo":"Short-term  UV-B  exposure  induces  metabolic  and  anatomical changes  in  peel  of  harvested  lemons  contributing  in  fruit  protection against green mold","resumen":"UV-B radiation (UVBR) is a small fraction of the solar spectrum from 280 to 315 nm. Low UVBR produces photomorphogenic acclimation responses in plants, modulating their cellular structure and physiology. Here, changes in peel of harvested lemons after short time exposure to UVBR were analyzed and its potential effects against fungal infection were studied. In the flavedo, UVBR treatment induced variations in the respiratory profiles and increased the phenolic compound contents. Final products of the flavonoid pathway (flavones, flavonols and anthocyanins) increased more markedly than their precursors (flavanones and dihydroflavonols). The increased accumulation of soluble phenolics in flavedo of treated lemons is associated with the high antioxidant activity found in the flavedo of these samples. Supporting the biochemical determinations, anatomical observations showed abundant intravacuolar deposits of phenolic compounds and an increase in the cell wall thickness in UVBR-treated samples. Metabolic and anatomical modifications associated to UVBR improved natural defenses against Penicillium digitatum, causal agent of green mold disease. Our results suggest that mature postharvest lemons exposed to the artificial radiation showed a phenotypic plasticity, allowing an acclimation response to UVBR which confer fruit resistance to pathogens. Thus, combination of UVBR with other treatments could represent an important improvement to control postharvest diseases on citrus.","palabras_clave":"UV-B Radiation |Penicillium digitatum|Postharvest|Citrus |phenolic Compounds"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4902,"idioma_id":5,"titulo":"Prácticas de lectura y escritura en el marco de la enseñanza de la psicología: avances de una investigación interdisciplinaria","resumen":"Este trabajo presenta los resultados obtenidos a partir de la realización de entrevistas a profesores de diez materias consideradas troncales en la carrera de Psicología de una universidad argentina. Se utilizó un cuestionario-base abierto a la interacción, que los interrogó sobre las prácticas de lectura y de escritura que promueven en sus aulas. También se les solicitaron muestras del material bibliográfico y consignas de escritura o de evaluación propuestas como requisitos de aprobación a fin de triangular información. Los resultados arrojaron un primer diagnóstico sobre las dificultades que los profesores tienen para guiar las prácticas de lectura y escritura que sus alumnos deben llevar a cabo. La interacción con los docentes promovió un proceso de reflexión que pudo dar inicio a una incidencia positiva en sus prácticas en el marco de la enseñanza de la carrera y sentar las bases para poner en marcha un programa de lectura y escritura académicas.","palabras_clave":"lecto-escritura|educación superior|estrategias de enseñanza"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4903,"idioma_id":4,"titulo":"Relationship between environmental conditions and host-seeking activity of Ochlerotatus albifasciatus (Diptera: Culicidae) in an agroecosystem and in an urban area in Chubut, Central Patagonia, Argentina","resumen":"Ochlerotatus albifasciatus is associated with aquatic environments that are frequently &#64258;ooded. Awareness of blood hematophagic activity of female mosquitoes is particularly important in species which are vectors of human disease and domestic animals. The study of host-seeking activity allows us to infer the risk of transmission of these parasites. The objective of this work was to study in an agro-ecosystem and urban area of central Argentine Patagonia the relationship between the circadian rhythm of activity of host-seeking of Oc. albifasciatus, and environmental variables. During January 2003, 99 human bait catches were carried out, of which 77.8% registered at least one individual of Oc. albifasciatus. Generalised linear models were used to evaluate the relationship between Oc. albifasciatus biting activity rate and capture time, environmental humidity, air temperature, sunshine degree, wind speed, habitat type and proximity of larval habitat. The model that best explained the variation in biting activity rate included the capture time, environmental humidity, air temperature, sunshine degree and wind speed as predictor variables. Hostseeking activity was higher in the evening than in the morning or afternoon, and it was positively related to mean air temperature and environmental relative humidity (RH), but negatively to sunshine degree and wind speed. The combination of these characteristics (wind speed less than 20 km\/h, environmental temperature greater than 26°C, sunshine less than 40% and (RH) greater than 50%) would provide favourable conditions to allow seeking activity and to feed on the host. The dispersion of this mosquito could be among environmental patches that present these characteristics.","palabras_clave":"Ochlerotatus|patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4904,"idioma_id":4,"titulo":"Addressing the role of conformational diversity in protein structure prediction","resumen":"Computational modeling of tertiary structures has become of standard use to study proteins that lack experimental characterization. Unfortunately, 3D structure prediction methods and model quality assessment programs often overlook that an ensemble of conformers in equilibrium populates the native state of proteins. In this work we collected sets of publicly available protein models and the corresponding target structures experimentally solved and studied how they describe the conformational diversity of the protein. For each protein, we assessed the quality of the models against known conformers by several standard measures and identified those models ranked best. We found that model rankings are defined by both the selected target conformer and the similarity measure used. 70% of the proteins in our datasets show that different models are structurally closest to different conformers of the same protein target. We observed that model building protocols such as template-based or ab initio approaches describe in similar ways the conformational diversity of the protein, although for template-based methods this description may depend on the sequence similarity between target and template sequences. Taken together, our results support the idea that protein structure modeling could help to identify members of the native ensemble, highlight the importance of considering conformational diversity in protein 3D quality evaluations and endorse the study of the variability of the native structure for a meaningful biological analysis.","palabras_clave":"Conformational diversity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4905,"idioma_id":4,"titulo":"CoDNaS 2.0: a comprehensive database of protein conformational diversity in the native state","resumen":"CoDNaS (conformational diversity of the native state) is a protein conformational diversity database. Conformational diversity describes structural differences between conformers that define the native state of proteins. It is a key concept to understand protein function and biological processes related to protein functions. CoDNaS offers a well curated database that is experimentally driven, thoroughly linked, and annotated. CoDNaS facilitates the extraction of key information on small structural differences based on protein movements. CoDNaS enables users to easily relate the degree of conformational diversity with physical, chemical and biological properties derived from experiments on protein structure and biological characteristics. The new version of CoDNaS includes 70% of all available protein structures, and new tools have been added that run sequence searches, display structural flexibility profiles and allow users to browse the database for different structural classes. These tools facilitate the exploration of protein conformational diversity and its role in protein function.","palabras_clave":"PROTEIN|CONFORMATIONS|STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4906,"idioma_id":4,"titulo":"Neural correlates of expression-independent memories in the crab Neohelice","resumen":"AbstractThe neural correlates of memoryhave been usually examined considering that memory retrieval and memoryexpression are interchangeable concepts. However, our studies in the crab Neohelice (Chasmagnathus)granulata and in other memory models haveshown that memory expression is not necessary for memory to bere-activated and become labile.Inorder to examine putative neuralcorrelates of memory in the crab Neohelice,we contrast changes induced by training in both animal´s behavior and neuronalresponses in the medulla terminalisusing in-vivo Ca2+ imaging. Disruption of long-term memory by the amnesic agentsMK-801 and scopolamine (5µg\/g) blocked the learning-induced changes in theCa2+ responses in the medulla terminalis. Conversely, treatments that lead to an unexpressedbut persistent memory (weak training protocol or scopolamine 0.1 µg\/g) do notblock these learning-induced neural changes. The present results reveal a setof changes in the neural activity induced by training that correlates withmemory persistence but not with the probability of this memory to be expressedin the long-term. In addition, the study constitutes the first in-vivo evidencein favor of a role of the medullaterminalis in learning and memory in crustaceans, and provides a physiologicalevidence indicating that memory persistence and the probability of memory to beexpressed might involve separate components of memory traces. &nbsp;&nbsp;","palabras_clave":"Learning|Memory|Calcium Imaging|Invertebrates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4907,"idioma_id":4,"titulo":"Sickness behavior in honey bees","resumen":"During an infection, animals suffer several changes in their normal physiology and behavior which may include lethargy, appetite loss, and reduction in grooming and general movements. This set of alterations is known as sickness behavior and although it has been extensively believed to be orchestrated primarily by the immune system, a relevant role for the central nervous system has also been established. The aim of the present work is to develop a simple animal model to allow studying how the immune and the nervous systems interact coordinately during an infection. We administered a bacterial lipopolysaccharide (LPS) into the thorax of honey bees to mimic a bacterial infection, and then we evaluated a set of stereotyped behaviors of the animals that might be indicative of sickness behavior. First, we show that this immune challenge reduces the locomotor activity of the animals in a narrow time window after LPS injection. Furthermore, bees exhibit a loss of appetite 60 and 90 min after injection, but not 15 h later. We also demonstrate that LPS injection reduces spontaneous antennal movements in harnessed animals, which suggests a reduction in the motivational state of the bees. Finally, we show that the LPS injection diminishes the interaction between animals, a crucial behavior in social insects. To our knowledge these results represent the first systematic description of sickness behavior in honey bees and provide important groundwork for the study of the interaction between the immune and the neural systems in an insect model.","palabras_clave":"HONEY BEES|SICKNESS BEHAVIOR|LIPOPOLYSACCHARIDE|LOCOMOTION|FEEDING|SOCIAL INTERACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4908,"idioma_id":4,"titulo":"Kinship and Phenotypic Divergence in the Ancient Population of the Puna Plateau of Northwestern Argentina","resumen":"The Puna de Jujuy is a characteristic region of Northwest Argentina, and archaeological evidence shows remarkable development in this area during the period of Regional Development (PRD) or Late period. The aim of this paper is to study the genetic structure of the late population of the Puna de Jujuy (Argentina) using cranial metric traits. The sample comprised 302 adult individuals belonging to six different localities: Agua Caliente, Casabindo, Doncellas, Queta, Río Negro and Sorcuyo. The research included 27 cranial metric variables, a discriminant analysis and the calculation of Mahalanobis? D2 distance and the fixation or genetic divergence index. The results showed little phenotypic differentiation between six locations; 1% of the genetic variation explains the differences between groups, and the rest represents variations within the group. The sites of Queta and Sorcuyo have lower observed variance values than expected in the model of balance between genetic drift and gene flow, and Agua Caliente showed higher observed variance values than expected. The remaining sites showed values close to the ones expected in the model. The calculated distances suggest that the differentiation between localities is moderate. These results are compared to FST calculated for other local populations. A differential distribution of morphological variability between sites was observed. Agua Caliente, Casabindo, Doncellas and Río Negro showed higher morphological variability due to higher gene flow values, whereas Queta and Sorcuyo showed lower morphological variability.","palabras_clave":"BIOLOGICAL AFFINITIES|LATE PERIOD|GENETIC DRIFT|GENE FLOW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4909,"idioma_id":4,"titulo":"Post-mortem findings in southern right whales (Eubalaena australis) at Península Valdés, Argentina, 2003 - 2012","resumen":"Between 2003 and 2012, 605 southern right whales (SRW; Eubalaena australis) were found dead along the shores of Península Valdés (PV), Argentina. These deaths included alarmingly high annual losses between 2007 and 2012, a peak number of deaths (116) in 2012, and a significant number of deaths across years in calves-of-the-year (544 of 605 [89.9%]; average = 60.4 yr(-1)). Post-mortem examination and pathogen testing were performed on 212 whales; 208 (98.1%) were calves-of-the-year and 48.0% of these were newborns or neonates. A known or probable cause of death was established in only a small number (6.6%) of cases. These included ship strike in a juvenile and blunt trauma or lacerations (n = 5), pneumonia (n = 4), myocarditis (n = 2), meningitis (n = 1), or myocarditis and meningitis (n = 1) in calves. Ante-mortem gull parasitism was the most common gross finding. It was associated with systemic disease in a single 1-2 mo old calf. Immunohistochemical labeling for canine distemper virus, Toxoplasma gondii and Brucella spp., and PCR for cetacean morbillivirus (CeMV), influenza A, and apicomplexan protozoa were negative on formalin-fixed, paraffin-embedded lung and brain samples from a subset of whales; PCR for Brucella spp. was positive in a newborn\/neonate with pneumonia. Skin samples from whales with gull parasitism were PCR negative for CeMV, poxvirus, and papillomavirus. This is the first long-term study to investigate and summarize notable post-mortem findings in the PV SRW population","palabras_clave":"enfermedades|acuaticos|infeciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4910,"idioma_id":5,"titulo":"Los prociónidos fósiles de América del Sur","resumen":"La familia Procyonidae (Mammalia, Carnivora) se encuentra representada en América del Sur por cinco géneros vivientes (Bassaricyon, Nasuella, Potos, Procyon y Nasua). De ellos, sólo Procyon y Nasua están presentes en los registros fósiles (Pleistoceno Tardío - Holoceno) en Argentina, Brasil y Bolivia. Los prociónidos sudamericanos actuales representan una segunda invasión desde América del Norte o Central durante el Pleistoceno Tardío-Holoceno y no están directamente relacionados con los géneros de prociónidos endémicos y extintos: ?Cyonasua y ?Chapalmalania. Estos últimos corresponderían a un primer episodio de migración (en el marco del Proto GABI) contando con registros en Argentina, Venezuela, Colombia y probablemente en Perú, durante el Mioceno Tardío al Pleistoceno Temprano. Estudios paleobiológicos sugieren diferencias craneodentarias y de tamaño entre los taxones extintos. A pesar de que presentan una morfología dentaria adaptada a dietas omnívoras, ?Cyonasua habría tenido una dieta más carnívora, mientras que ?Chapalmalania hábitos carroñeros. La mayoría de las especies ?Cyonasua son relativamente más grandes de tamaño que los prociónidos actuales (13 a 25 Kg.;  siendo el doble o triple que al de los prociónidos vivientes), mientras que ?Chapalmalania podría haber sido mucho más grande. Estudios de preferencias de sustrato y modos locomotores infieren que ?Cyonasua tendría hábitos terrestres y posiblemente capacidades para trepar.","palabras_clave":"PROCYONIDAE|FÓSIL|EVOLUCIÓN|PALEOBIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4911,"idioma_id":4,"titulo":"Reading shell shape: implications for palaeoenvironmental reconstructions. A case study for bivalves from the marine Quaternary of Argentina (south-western Atlantic)","resumen":"Most research on bivalves from the south-western Atlantic used morphological (shell) characters for taxonomic discriminations. Dominant Veneroids from Argentinian Quaternary coastal deposits exhibit wide morphological variation ?  often making objective discriminations difficult\/impossible, which could be objectively described and compared through geometric morphometrics techniques. This work focuses on comparison of geometric morphometrics methods applied to  fossil and modern shells, to assess inter- and intra-generic variations. Three approaches were considered: landmarks (L), semi-landmarks (SL) and outlines. Shell shape analyses for different time spans (Pleistocene, fossil Holocene and modern) and areas (Patagonia and Bonaerensian) showed that Elliptic Fourier analysis (EFA), Landmarks and Landmarks plus Semilandmarks (L þ SL) can discriminate at generic levels: Mactra, Mulinia (Mactridae) vs. Pitar, Protothaca, Eurhomalea, Clausinella (Veneridae). L and L þ SL are powerful for inter\/intraspecific distinctions of Mactra. Variability of Mactra isabelleana includes the remaining nominal ?species? (transitional morphs). Causal environmental factors of (phenotype) variation could be addressed for modern environments (substrate, salinity and energy). Subtrigonal-inflated shells predominate in muddy, quieter, shallow mixo-polyhaline waters; ovate-elongate-compressed in sandy, poly-euhaline,  deeper habitats. Differential spatial distribution (and abundance) across time responds to Late Quaternary high sea-level  stands: transgressive maxima allowed higher salinity in marginal-marine areas and optimal conditions for Mactra  isabelleana contrasting with scarcer records in the Mar Argentino today.","palabras_clave":"MOLLUSCS|HOLOCENE|PLEISTOCENE|VARIABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4912,"idioma_id":4,"titulo":"Dissecting galaxy triplets in the Sloan Digital Sky Survey Data Release 10 - I. Stellar populations and emission line analysis","resumen":"We identify isolated galaxy triplets in a volume-limited sample from the SloanDigital Sky Survey Data Release 10. Our nal sample has 80 galaxy systems in theredshift range 0.046z60.1, brighter than Mr = &#1048576;20:5 + 5 log h70. Spectral synthe-sis results and WHAN and BPT diagnostic diagrams were employed to classify thegalaxies in these systems as star-forming, active nuclei, or passive\/retired.Our results suggest that the brightest galaxies drive the triplet evolution, as ev-idenced by the strong correlations between properties as mass assembly and meanstellar population age with triplet properties. Galaxies with intermediate luminosityor the faintest one within the triplet seem to play a secondary role. Moreover, therelation between age and stellar mass of galaxies is similar for these galaxies but dif-ferent for the brightest galaxy in the system. Most of the triplet galaxies are passiveor retired, according to the WHAN classication. Low mass triplets present dierentfractions of WHAN classes when compared to higher mass triplets. A census of WHANclass combinations shows the dominance of star-forming galaxies in low mass tripletswhile retired and passive galaxies prevail in high-mass systems. We argue that theseresults suggest that the local environment, through galaxy interactions driven by thebrightest galaxy, is playing a major role in triplet evolution.","palabras_clave":"GALAXIES: EVOLUTION|GALAXIES: STELLAR CONTENT |GALAXIES: STATISTICS|GALAXIES: ACTIVE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4913,"idioma_id":4,"titulo":"Low X-Ray Luminosity Galaxy Clusters: Main goals, sample selection, photometric and spectroscopic observations","resumen":"We present the study of nineteen low X-ray luminosity galaxy clusters (LX &#8764; 0.5?45 × 1043 erg s&#8722;1),selected from the ROSAT Position Sensitive Proportional Counters (PSPC) Pointed Observations (Vikhlinin et al. 1998) and the revised version of Mullis et al. (2003) in the redshift range of 0.16 to 0.7.This is the introductory paper of a series presenting the sample selection, photometric and spectroscopic observations and data reduction. Photometric data in different passbands were taken for eight galaxy clusters at Las Campanas Observatory; three clusters at Cerro Tololo Interamerican Observatory; and eight clusters at the Gemini Observatory. Spectroscopic data were collected for only four galaxy clusters using Gemini telescopes.With the photometry, the galaxies were defined based on the star-galaxy separation taking into account photometric parameters. For each galaxy cluster, the catalogues contain the PSF and aperture magnitudes of galaxies within the 90% completeness limit. They are used together with structural parameters to study the galaxy morphology and to estimate photometric redshifts. With the spectroscopy, the derived galaxy velocity dispersion of our clusters ranged from 507 km s&#8722;1 for [VMF98]022to 775 km s&#8722;1 for [VMF98]097 with signs of substructure.Cluster membership has been extensively discussed taking into account spectroscopic and photometric redshift estimates. In this sense, members are the galaxies within a projected radius of 0.75 Mpc from the X-ray emission peak and with clustercentric velocities smaller than the cluster velocity dispersion or 6000 km s&#8722;1, respectively. These results will be used in forthcoming papers to study, among the main topics, the red cluster sequence, blue cloud and green populations; the galaxy luminosity function and cluster dynamics.","palabras_clave":"LOW|LUMINOSITY|GALAXY|CLUSTERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4914,"idioma_id":4,"titulo":"Distribution of Telomeric Sequences (TTAGGG) n in Rearranged Chromosomes of Phyllotine Rodents (Cricetidae, Sigmodontinae).","resumen":"Phyllotine are sigmodontine rodents endemic to South America with broad genetic variability, being Robertsonian polymorphisms the most frequent. Moreover, this taxon includes a species with multiple sex chromosomes, which is infrequent in mammals. However, molecular cytogenetic techniques were never applied to phyllotines to study its karyotypic evolution. We study the chromosome of four phyllotine species using FISH with a pantelomeric probe (TTAGGG)n. Graomys griseoflavus, Eligmodontia puerulus and E. morgani are polymorphic for Robertsonian translocations; whereas Salinomys delicatus posses XX\/XY1Y2 sex chromosomes. Telomeric signals were detected at both ends of all chromosomes of studied species. In S. delicatus interstitial telomeric sequences (ITS) were observed in the three major chromosome pairs, which are equidistant from one of the telomeres in these chromosomes. These results suggest that ITS are important in the reshuffling of the highly derived karyotype of S. delicatus. Considering the phylogeny of phyllotines, the Robertsonian rearrangements of G. griseoflavus, E. puerulus and E. morgani possibly represent chromosome fusions which have occurred independently. The pericentromeric regions of the biarmed chromosomes of these species do not contain the telomeric sequences characteristic of strict fusions of recent origin, suggesting a common pattern of telomeric repeats loss during the chromosomal evolution of these rodents.","palabras_clave":"TELOMERES|ROBERTSONIAN REARRANGEMENTS|RODENTS|SOUTH AMERICA|PHYLLOTINI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4916,"idioma_id":4,"titulo":"Shock Waves and Commutation Speed of Memristors","resumen":"Progress of silicon-based technology is nearing its physical limit, as the minimum feature size ofcomponents is reaching a mere 10 nm. The resistive switching behavior of transition metal oxides and theassociated memristor device is emerging as a competitive technology for next-generation electronics.Significant progress has already been made in the past decade, and devices are beginning to hit the market;however, this progress has mainly been the result of empirical trial and error. Hence, gaining theoreticalinsight is of the essence. In the present work, we report the striking result of a connection between theresistive switching and shock-wave formation, a classic topic of nonlinear dynamics. We argue that theprofile of oxygen vacancies that migrate during the commutation forms a shock wave that propagatesthrough a highly resistive region of the device.We validate the scenario by means of model simulations andexperiments in a manganese-oxide-based memristor device, and we extend our theory to the case of binaryoxides. The shock-wave scenario brings unprecedented physical insight and enables us to rationalizethe process of oxygen-vacancy-driven resistive change with direct implications for a key technologicalaspect?the commutation speed.","palabras_clave":"memristors|non volatile memory |schock waves"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4917,"idioma_id":4,"titulo":"Failure of a single dose of medroxyprogesterone acetate to induce uterine infertility in postnatally treated domestic cats","resumen":"In mice and sheep, neonatal administration of progesterone or progestins inhibited development of uterine glands. The aims of the present study were (1) to describe uterine gland development on postnatal Days 6 to 8 and (2) to evaluate the effects of a single postnatal administration of a progestin on reproduction and adult uterine glands morphology and function in domestic cats. Necropsy was performed on three 1-week-old female cats which had died unrelated to this study. Ten female kittens were randomly assigned within the first 24 hours of birth to: medroxyprogesterone acetate 10 mg\/animal subcutaneously (MPA; n = 6) or placebo (PLC; n = 4) and followed up until puberty when they were mated. Twenty-four days after the end of estrus, ovulation and pregnancy were diagnosed by serum progesterone measurement and ultrasonography, respectively. Then, all the cats were ovariohysterectomized. After necropsy or surgery, the excised organs were histologically evaluated. Seven queens ovulated (4 of 6 MPA and 3 of 4 PLC; P > 0.1) and were pregnant (P > 0.1). Four MPA cats presented endometrial hyperplasia and one of them developed a pyometra. The 1-week-old females presented uterine glands in the stage of budding and incipient penetration of the glandular epithelium into the underlying stroma. The MPA-treated queens revealed that the area occupied by uterine glands per square-micrometer (0.55 ± 0.2 vs. 0.49 ± 0.2; P > 0.1) and the height of the glandular epithelium (&#956;m; 24.5 ± 6.7 vs. 24.4 ± 7.2; P > 0.1) did not differ from those of the PLC group. Neither significant gross nor microscopical differences were also found for ovaries (P > 0.1). It is concluded that 1-week-old kittens had an incipient stage of uterine gland development and that a single postnatal supraphysiological dose of MPA did not alter uterine adenogenesis in this species. Furthermore, this treatment seemed to predispose to uterine disease without prevention of fertility.","palabras_clave":"FELID|ENDOCRINE DISRUPTOR|GENITAL TRACT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4918,"idioma_id":4,"titulo":"Study of the Eosin-Y\/PAMAM interactions in alkaline aqueous solution","resumen":"The interactionsbetweenthexanthenedyeEosin-Y(Eos)andamino-terminated PAMAM dendrimers of low generations(G0?G3) were studied in alkaline water olution. The effect of concentration and generation of the dendrime r on the photophysics of Eos was evaluated by means of absorption and fluorescence&nbsp; spectroscopies. Theo bserved spectral changes were ascribed to the association dye\/dendrimer.From these data, the Eos\/PAMAM binding constants (Kbind) were determined, which strongly increased with the size of the dendrimer. Stationary fluorescence anisotropy and ime-resolved single photon counting were also used to characterize the association process. Theres triction in the rotational diffusion of the Eos increased as a function of the concentration and generation of PAMAM, as determined by anisotropy measurements. Biexponential fluorescence decays were obtained in the presence of G3, andthe respective lifetimes were ascribed to free and bound Eos species. These esults correlate with Kbind values ands uggest the formation of host\/guest system with larger dendrimers. Therefore, this environmentally-friendly dye\/dendrimer system would be appropriate for potential applications in fields such asdrugsdeliveryandphotopolymerization.","palabras_clave":"PAMAM dendrimers|Eosin-Y|Dye\/dendrimer association|Host\/guest system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4919,"idioma_id":4,"titulo":"Inhalation of growth factors and apo-transferrin to protect and repair the hypoxic-ischemic brain","resumen":"AbstractHypoxic-ischemic brain damage is a major contributor to chronic neurological dysfunction and acute mortality in infants as well as in adults. In this review, we summarize recent publications demonstrating that the intranasal administration (INA) of apo-transferrin (aTf) and different growth factors provides neuroprotection to the mouse and rat brain after a hypoxic-ischemic event. The intranasal delivery of growth factors such as insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) has been found to improve neurological function and reduce infarct size in adult rats after a hypoxic-ischemic event. On the other hand, INA of aTf and epidermal growth factor (EGF) were effective in reducing white matter damage and inflammation and in promoting the proliferation and survival of oligodendroglial progenitor cells (OPCs) in a model of hypoxic-ischemic encephalopathy. Therefore, data summarized in this review suggest that INA of growth factors and aTf can be used in combination in clinical treatment in order to protect and repair the hypoxic-ischemic brain. ","palabras_clave":"APO-TRANSFERRIN|HYPOXIC|ISCHEMIC|BRAIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4920,"idioma_id":4,"titulo":"Kinetic Modeling of Sunflower Grain Filling and Fatty Acid Biosynthesis","resumen":"Grain growth and oil biosynthesis are complex processes that involve various enzymes placed in different sub-cellular compartments of the grain. In order to understand the mechanisms controlling grain weight and composition, we need mathematical models capable of simulating the dynamic behavior of the main components of the grain during the grain filling stage. In this paper, we present a non-structured mechanistic kinetic model developed for sunflower grains. The model was first calibrated for sunflower hybrid ACA855. The calibrated model was able to predict the theoretical amount of carbohydrate equivalents allocated to the grain, grain growth and the dynamics of the oil and non-oil fraction, while considering maintenance requirements and leaf senescence. Incorporating into the model the serial-parallel nature of fatty acid biosynthesis permitted a good representation of the kinetics of palmitic, stearic, oleic, and linoleic acids production. A sensitivity analysis showed that the relative influence of input parameters changed along grain development. Grain growth was mostly affected by the specific growth parameter (&#956;&#8242;) while fatty acid composition strongly depended on their own maximum specific rate parameters. The model was successfully applied to two additional hybrids (MG2 and DK3820). The proposed model can be the first building block toward the development of a more sophisticated model, capable of predicting the effects of environmental conditions on grain weight and composition, in a comprehensive and quantitative way.","palabras_clave":"FATTY ACIDS BIOSYNTHESIS|GRAIN FILLING|KINETIC MODEL|SUNFLOWER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4922,"idioma_id":4,"titulo":"Comparative characterization of Shiga toxin type 2 and Subtilase cytotoxin effects on human renal epithelial and endothelial cells grown in monolayer and bilayer conditions","resumen":"Postdiarrhealhemolytic uremic syndrome (HUS) affects children under 5 years old and is responsiblefor the development of acute and chronic renal failure, particularly inArgentina. This pathology is a complication of Shiga toxin (Stx)-producingEscherichia coli infection and renal damage is attributed to Stx types 1 and 2(Stx1, Stx2) produced by Escherichia coli O157:H7 and many other STECserotypes. It has been reported the production of Subtilase cytotoxin (SubAB)by non-O157 STEC isolated from cases of childhood diarrhea. Therefore, it isproposed that SubAB may contribute to HUS pathogenesis. The human kidney is themost affected organ because very Stx-sensitive cells express high amounts of biologicallyactive receptor. In this study, we investigated the effects of Stx2 and SubABon primary cultures of human glomerular endothelial cells (HGEC) and on a humantubular epithelial cell line (HK-2) in monoculture and coculture conditions. Wehave established the coculture as a human renal proximal tubule model to studywater absorption and cytotoxicity in the presence of Stx2 and SubAB. Weobtained and characterized cocultures of HGEC and HK-2. Under basal conditions,HGEC monolayers exhibited the lowest electrical resistance (TEER) and thehighest water permeability, while the HGEC\/HK-2 bilayers showed the highestTEER and the lowest water permeability. In addition, at times as short as 20?30minutes, Stx2 and SubAB caused the inhibition of water absorption across HK-2and HGEC monolayers and this effect was not related to a decrease in cellviability. However, toxins did not have inhibitory effects on water movementacross HGEC\/HK-2 bilayers. After 72 h, Stx2 inhibited the cell viability ofHGEC and HK-2 monolayers, but these effects were attenuated in HGEC\/HK-2bilayers. On the other hand, SubAB cytotoxicity shows a tendency to beattenuated by the bilayers. Our data provide evidence about the differenteffects of these toxins on the bilayers respect to the monolayers. This invitro model of communication between human renal microvascular endothelial cellsand human proximal tubular epithelial cells is a representative model of thehuman proximal tubule to study the effects of Stx2 and SubAB related to thedevelopment of HUS.","palabras_clave":"SHIGA TOXIN TYPE 2|SUBTILASE CYTOTOXIN|RENAL EPITHELIAL CELLS|RENAL ENDOTHELIAL CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4923,"idioma_id":4,"titulo":"Aminopropyltrimethoxysilane- and aminopropyltrimethoxysilane-silver-modified montmorillonite for the removal of nitrate ions","resumen":"In this work, a natural clay (Mn) modified with &#947;-aminopropyltrimethoxysilane (Mn-S) and silver (Mn-S-Ag) were evaluated as a potential adsorption media for nitrate removal from water. The natural and modified clays were characterised by FTIR, XRD, zeta-potential, TGA\/DTA, SEM and elemental analyses. First, the adsorption capacity of the Mn-S clay for Ag(+) was studied. From the results, the cation adsorption capacity was almost three times greater for Mn-S than for Mn because a metal-amino group complex formation is favored when silver cations and amino groups (-NH2) are contacted. Second, the nitrate adsorption on Mn-S and Mn-S-Ag were studied as a function of contact time, pH, temperature and adsorbent dosage using batch techniques. In the absence of Ag(+), NO3(-) adsorption was high at low pH and the amount of adsorbed nitrate decreased as the pH increased, which indicates the presence of electrostatic interactions between the anion and protonated amine groups. The results showed that both clays (Mn-S and Mn-S-Ag) can retain an important amount of nitrate ions compared with previously reported data. For the Mn-S clay, the following monolayer adsorption capacities were obtained: Qmax=0.80, 0.72 and 0.68 mmol NO3(-)\/g at pH 3, 5, and 7, respectively; whereas for the Mn-S-Ag clay, the values obtained were: Qmax= 0.77, 0.74 and 0.42 mmol NO3(-)\/g at pH 3, 5, and 7, respectively. From the results obtained in this work, NO3(-) adsorption occurs over -NH3(+) surface groups without involving the Ag(+) cation in the adsorption process. This cation is not a promoter of nitrate adsorption. Aminopropyltrimethoxysilane- and aminopropyltrimethoxysilane-silver-modified montmorillonite for the removal of nitrate ions. Available from: https:\/\/www.researchgate.net\/publication\/296624200_Aminopropyltrimethoxysilane-_and_aminopropyltrimethoxysilane-silver-modified_montmorillonite_for_the_removal_of_nitrate_ions [accessed May 4, 2017].","palabras_clave":"Adsorption; |nitrate;|aminopropyltrimethoxysilane|montmorillonite;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4924,"idioma_id":4,"titulo":"Role of amphipathicity and hydrophobicity in the balance between hemolysis and peptide-membrane interactions of three related antimicrobial peptides","resumen":"Cationic antimicrobial peptides (CAMPs) represent important self defense molecules in many organisms, including humans. These peptides have a broad spectrum of activities, killing or neutralizing many Gram-negative and Gram-positive bacteria. The emergence of multidrug resistant microbes has stimulated research on the development of alternative antibiotics. In the search for new antibiotics, cationic antimicrobial peptides (CAMPs) offer a viable alternative to conventional antibiotics, as they physically disrupt the bacterial membranes, leading to lysis of microbial membranes and eventually cell death. In particular, the group of linear &#945;-helical cationic peptides has attracted increasing interest from clinical as well as basic research during the last decade.In this work, we studied the biophysical and microbiological characteristics of three new designed CAMPs. We modified a previously studied CAMP sequence, in order to increase or diminish the hydrophobic face, changing the position of two lysines or replacing three leucines, respectively. These mutations modified the hydrophobic moment of the resulting peptides and allowed us to study the importance of this parameter in the membrane interactions of the peptides. The structural properties of the peptides were also correlated with their membrane-disruptive abilities, antimicrobial activities and hemolysis of human red blood cells.","palabras_clave":"CAMPs|Membrane interaction|Hydrophobicity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4925,"idioma_id":4,"titulo":"Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields","resumen":"Alfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRVArg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV genome of the Manfredi isolate (Manfredi BLRV-Arg) is 5884 nucleotides in length, with a genome organization similar to that already reported for this virus. The complete nucleotide sequence or selected genes were subjected to sequence comparisons. Manfredi BLRV-Arg had more than 96 % nucleotide and amino acid sequence identities, both for complete genome sequence and ORFs, with those of the two full-length genome sequences available in GenBank. The complete CP sequences of Manfredi BLRV-Arg showed an identity percentage over 98 % at nt and deduced aa level when were compared with those of the other CP complete sequences available in GenBank. This is the first evidence for the presence of BLRV in Argentina, its geographical distribution in alfalfa production areas and the complete genome sequence of an isolate fromalfalfa as natural host.","palabras_clave":"MEDICAGO SATIVA|LUTEOVIRUS|COMPLETE SEQUENCE|FIEL DETECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4926,"idioma_id":4,"titulo":"Complete genome sequence of a new enamovirus from Argentina infecting alfalfa plants showing dwarfism symptoms","resumen":"Alfalfa dwarf disease, probably caused by synergistic interactions of mixed virus infections, is a major and emergent disease that threatens alfalfa production in Argentina. Deep sequencing of diseased alfalfa plant samples from the central region of Argentina resulted in the identification of a new virus genome resembling enamoviruses in sequence and genome structure. Phylogenetic analysis suggests that it is a new member of the genus Enamovirus, family Luteoviridae. The virus is tentatively named ??alfalfa enamovirus 1?? (AEV-1). The availability of the AEV-1 genome sequence will make it possible to assess the genetic variability of this virus and to construct an infectious clone to investigate its role in alfalfa dwarfism disease.","palabras_clave":"Enamovirus|Complete genome|Alfalfa|Dwarfism"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4928,"idioma_id":4,"titulo":"Demographic dynamics of Akodon azarae (Cricetidae: Sigmodontinae) in linear habitats of agricultural landscapes of central Argentina","resumen":"Linear habitats are capable of providing essential resources for small mammals within the 22 agroecosystems. We analyzed demographic parameters of Akodon azarae in a linear habitat of 23 central Argentina. We used CMR models to determine temporal variations in survival, recruitment 24 and population growth rate. The effects of temperature, rainfall and vegetation cover as well as of 25 population abundances on the mentioned parameters were assessed in a ten-year study. Survival and 26 growth rate showed seasonal variation, which was greater than the interannual variability observed. 27 Temperature affected only recruitment. Linear habitats would provide resources for long term 28 survival and reproduction of A. azarae.","palabras_clave":"CMR|population growth rate|recruitment|survival|temporal variation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4929,"idioma_id":4,"titulo":"First report of Cowpea mild mottle virus in chia (Salvia hispanica)","resumen":"Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its highconcentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptomswere observed in the principal chia production areas in Argentina. Some plants exhibited yellowing,mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aimof this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collectedfrom nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus(AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV)and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Bego-movirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants,feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 toORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt)sequence of the coat-protein gene (CP e ORF5) of the chia virus isolate was obtained and compared with31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity,which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species dif-ferentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chiaisolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, PuertoRico, Taiwan and USA, but were separated from four others from India. This is the first report of thepresence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it ispossible that the different symptoms observed could be produced by a mixture of viruses becauseCPMMV was found in chia plants with different symptoms.","palabras_clave":"CARLAVIRUS|CPMMV|LAMIACEAE|PLANT VIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4930,"idioma_id":4,"titulo":"Development of Agro-climatic Zoning Model to delimit the potential growing areas for macaw palm (Acrocomia aculeata).","resumen":"The growing biodiesel production requires the use of new technologies and alternative feedstocks to maintain the growing demand of this biofuel. The macaw (Acrocomia aculeata) is a palm native to Argentina whose fruits present highoil content. Due to its tolerance to prolonged drought, it is a promising crop for biodiesel and biokerosene production. The aim of this work was to design an agroclimatic zoning model to define the potential growing areas from macaw in Argentina.To define the agroclimatic suitability to produce oil, it was necessary to identify the requirements, limits, and biometeorological tolerance for this palm. In order to define the agroclimatic fitness of this crop in Argentina, the meteorological data corresponding to the period 1981?2010 were employed. The agroclimatic indices were integrated in a Geographic Information System. Themaps were superimposed and the overlapping regions delineated the agroclimatic zoning. The agroclimatic zonation classified zoneswith homogeneous characteristics responding to bioclimatic requirements of this species, resulting in optimal, very suitable, suitable, and nonsuitable areas for macaw cultivation. The authors designed an agroclimatic zoning model based on bibliography.This model can be used in any part of the world, employing the same agroclimatic indices presented in this work.","palabras_clave":"Acrocomia aculeata|non-traditional bioenergy crop|bioclimatic requirements|agroclimatic zoning model|Geographic Information System|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4931,"idioma_id":4,"titulo":"Gap-junctional channel and hemichannel activity of two recently identified connexin 26 mutants associated with deafness","resumen":"Gap-junction channels (GJCs) are formed by head-to-head association of two hemichannels (HCs, connexin hexamers). HCs and GJCs are permeable to ions and hydrophilic molecules of up to Mr ~1 kDa. Hearing impairment of genetic origin is common, and mutations of connexin 26 (Cx26) are its major cause. We recently identified two novel Cx26 mutations in hearing-impaired subjects, L10P and G109V. L10P forms functional GJCs with slightly altered voltage dependence and HCs with decrease ATP\/cationic dye selectivity. G109V does not form functional GJCs, but forms functional HCs with enhanced extracellular Ca2+ sensitivity and subtle alterations in voltage dependence and ATP\/cationic dye selectivity. Deafness associated with G109V could result from decreased GJCs activity, whereas deafness associated to L10P may have a more complex mechanism that involves changes in HC permeability.","palabras_clave":"DEAFNESS|CONNEXINS|GAP-JUNCTION CHANNELS|MUTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4932,"idioma_id":4,"titulo":"MLPA analysis of an Argentine cohort of patients with dystrophinopathy: Association of intron breakpoints hot spots with STR abundance in DMD gene","resumen":"Dystrophinopathies are X-linked recessive diseases caused by mutations in the DMDgene. Our objective was to identify mutations in this gene by Multiplex Ligation Probe Amplification (MLPA), to confirm the clinical diagnosis and determine the carrier status of at-risk relatives. Also, we aimed to characterize the Dystrophinopathies argentine population and the DMD gene. We analyzed a cohort of 121 individuals (70 affected boys, 11 symptomatic women, 37 at-risk women and 3 male villus samples). The MLPA technique identified 56 mutations (45 deletions, 9 duplications and 2 point mutations). These results allowed confirming the clinical diagnosis in 63% (51\/81) of patients and symptomatic females. We established the carrier status of 54% (20\/37) of females at-risk and 3 male villus samples. We could establish an association between the most frequent deletion intron breakpoints and the abundance of dinucleotide microsatellites loci, despite the underlying mutational molecular mechanism remains to be elucidated. The MLPA demonstrate, again, to be the appropriate first mutation screening methodology for molecular diagnosis of Dystrophinopathies. The reported results permitted to characterize the Dystrophinopathies argentine population and lead to better understanding of the genetic and molecular basis of rearrangements in the DMD gene, useful information for the gene therapies being developed.","palabras_clave":"DUCHENNE MUSCULAR DYSTROPHY|MOLECUALR DIAGNOSIS|CARRIER DETECTION|GENE CHARACTERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4933,"idioma_id":4,"titulo":"Randomness and non-locality","resumen":"The concepts of randomness and non-locality are intimately intertwined outcomes of randomly chosen measurements over entangled systems exhibiting non-local correlations are, if we preclude instantaneous influence between distant measurement choices and outcomes, random. In this paper, we survey some recent advances in the knowledge of the interplay between these two important notions from a quantum information science perspective.","palabras_clave":"QUANTUM INFORMATION|QUANTUM NON-LOCALITY|RANDOMNESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4934,"idioma_id":4,"titulo":"Spin contamination-free N -electron wave functions in the excitation-based configuration interaction treatment","resumen":"This work deals with the spin contamination in N-electron wave functions provided by the excitation-based configuration interaction methods. We propose a procedure to ensure a suitable selection of excited N-electron Slater determinants with respect to a given reference determinant, required in these schemes. The procedure guarantees the construction of N-electron wave functions which are eigenfunctions of the spin-squared operator S^2, avoiding any spin contamination. Our treatment is based on the evaluation of the excitation level of the determinants by means of the expectation value of an excitation operator formulated in terms of spin-free replacement operators. We report numerical determinations of energies and &lt;S^2&gt; expectation values, arising from our proposal as well as from traditional configuration interaction methods, in selected open-shell systems, in order to compare the behavior of these procedures and their computational costs.","palabras_clave":"-"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4936,"idioma_id":4,"titulo":"From Hospital to Home-Care: Domotic environment for eldery and disabled people","resumen":"In this article, we present an assistive home care system specially designed for people with severe brain or spinal injuries and for poststroke survivors who are immobilized or face mobility difficulties. The main objective is to create the easy, low-cost automation of a room or house to provide a friendly environment that enhances the psychological condition of immobilized users","palabras_clave":"ASSISTIVE HOME CARE|DOMOTIC|HUMAN COMPUTER INTERFACE| ELECTROMYOGRAM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4937,"idioma_id":4,"titulo":"Heterogeneous expression pattern of tandem duplicated sHsps genes during fruit ripening in two tomato species","resumen":"The small heat shock proteins (sHSPs) have been found to play a critical role in physiological stress conditions in protecting proteins from irreversible aggregation. To characterize the gene expression profile of four sHsps with a tandem gene structure arrangement in the domesticated Solanum lycopersicum (Heinz 1706) genome and its wild close relative Solanum pimpinellifolium (LA1589), differential gene expression analysis using RNA-Seq was conducted in three ripening stages in both cultivars fruits. Gene promoter analysis was performed to explain the heterogeneous pattern of gene expression found for these tandem duplicated sHsps. In silico analysis results contribute to refocus wet experiment analysis in tomato sHsp family proteins.","palabras_clave":"sHSP|promoters|Solanum lycopersicum|Solanum pimpinellifolium"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4938,"idioma_id":4,"titulo":"Formal modeling of Gene Ontology annotation predictions based on factor graphs","resumen":"Gene Ontology (GO) is a hierarchical vocabulary for gene product annotation. Its synergy with machine learning classification methods has been widely used for the prediction of protein functions. Current classification methods rely on heuristic solutions to check the consistency with some aspects of the underlying GO structure. In this work we formalize the GO is-a relationship through predicate logic. Moreover, an ontology model based on Forney Factor Graph (FFG) is shown on a general fragment of Cellular Component GO.","palabras_clave":"Go ontology|Factor graph|predicate logic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4939,"idioma_id":4,"titulo":"Wearable System for Acquisition and Monitoring of Biological Signals","resumen":"This paper presents a modular, wearable system for acquisition and wireless transmission of biological signals. Configurable slaves for different signals (such as ECG, EMG, inertial sensors, and temperature) based in the ADS1294 Medical Analog Front End are connected to a Master, based in the CC3200 microcontroller, both from Texas Instruments. The slaves are configurable according to the specific application, providing versatility to the wearable system. The battery consumption is reduced, trough a couple of Li-ion batteries and the circuit has also a battery charger. A custom made box was designed and fabricated in a 3D printer, preserving the requirements of low cost, low weight and safety recommendations.","palabras_clave":"WEARABLE SYSTEMS|WIRELESS TRANSMISSION|BIOLOGICAL SIGNALS|EMG ACQUISITION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4940,"idioma_id":4,"titulo":"Recent developments in plant oil based functional materials","resumen":"&amp;amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:Times;panose-1:2 0 5 0 0 0 0 0 0 0;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;mso-bidi-font-size:10.0pt;font-family:'Times New Roman';mso-ascii-font-family:Courier;mso-fareast-font-family:Courier;mso-hansi-font-family:Courier;mso-bidi-font-family:'Times New Roman';}@page Section1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;} \/* List Definitions *\/@list l0{mso-list-id:295376129;mso-list-type:hybrid;mso-list-template-ids:-1125374638 271469648 67698713 67698715 67698703 67698713 67698715 67698703 67698713 67698715;}@list l0:level1{mso-level-start-at:26;mso-level-tab-stop:36.0pt;mso-level-number-position:left;text-indent:-18.0pt;mso-ansi-font-size:12.0pt;font-family:Times;mso-ansi-font-weight:normal;mso-ansi-font-style:normal;}ol{margin-bottom:0cm;}ul{margin-bottom:0cm;}--&amp;amp;gt;&nbsp;Mirna A. Mosiewicki, Mirta I. Aranguren 'Recent Developments in Plant Oil Based Functional Materials'.&nbsp; This is a comissioned review paper.&nbsp; Polymer International 65&nbsp;(1), 2838&nbsp; (January2016)&nbsp; On line: 27 Oct 2015&nbsp;&nbsp;&nbsp; doi: 10.1002\/pi.5033&lt;!-- \/* Font Definitions *\/@font-face{font-family:'Cambria Math';panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;mso-bidi-font-size:10.0pt;font-family:Courier;mso-fareast-font-family:Courier;mso-bidi-font-family:'Times New Roman';}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Courier;mso-ascii-font-family:Courier;mso-fareast-font-family:Courier;mso-hansi-font-family:Courier;}@page WordSection1{size:612.0pt 792.0pt;margin:72.0pt 72.0pt 72.0pt 72.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&gt;&nbsp;Theincreasing interest of academic and industrial sectors in the use of bio-based materialsmirrors the overwhelming need for replacing, as much as possible, petroleumderived chemicals, reducing the negative environmental impact derived from theirusage. Vegetable oils fulfill extremely well this goal, because of their worldwideavailability, large volume production at comparatively low prices and versatilityof the modifications and reactions in which they can participate to produce alarge variety of different monomers and polymer precursors. Further reactionsof these chemicals can lead to very different types of final materials with variedapplications. It is because of this remarkable versatility that many review articleshave appeared during the last years; many of them have dealt with the various routesfor vegetable oil modification and options for polymer synthesis, while others werededicated to the analysis of the properties of the derived materials, generallyfocusing on structural properties. In this review, we have focused on thecapabilities of vegetable oils to be modified and\/ or reacted to obtainmaterials with functional properties suitable to be used in coatings,conductive or insulating materials, biomedical, shape memory, self-healing andthermoreversible materials as well as other special functional applications.","palabras_clave":"VEGETABLE OILS|BIO-RESINS|FUNCTIONAL PROPERTIES|BIO-BASED POLYMERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4941,"idioma_id":4,"titulo":"Magnetism and Structure of Nanocomposites Made From Magnetite and Vegetable Oil Based Polymeric Matrices","resumen":"The aim of this work is to study the influence of the polymericmatrix composition on particle aggregation, magnetic interparticleinteractions and nanoparticle surface effects, which affect the magneticand structural properties of different ultra-diluted magnetitenanocomposites (MNCPs). Bio-based matrices were selected as a possibleresponse to the increasing demand for renewable materials. To investigatethe influence of different bio-based polymeric matrices on the magneticbehavior, three different bio-based polymers were used to prepare MNCPswith 1 wt.% of magnetite nanoparticles (MNPs). One of them was preparedusing a tung oil (TO)\/styrene (St) weight ratio of 70\/30, a second onewas prepared by replacing the styrene with methylester (green comonomerobtained from tung oil, ME, 70TO\/30ME) and a third one that incorporateda green modifier, acrylated epoxidized soybean oil (AESO), using a tungoil\/AESO weight ratio of 90\/10. Structural features as nanoparticleaggregation state, nanoparticle and cluster sizes, and fractal dimensionwere studied and determined from small-angle X-ray Scattering (SAXS). Theexperimental SAXS data were analyzed by means of fractal aggregate model.Results indicate differences in nanoparticle arrangement depending of thecontaining matrix. The magnetic characterization of these materialsindicates that the matrix strongly affects the physical and chemicalproperties of the MNCPs. All samples display superparamagnetic behaviorat room temperature, but the blocking temperature varies from 75 K (tungoil\/styrene with 1 wt.% MNPs) to 126 K (tung oil\/AESO 1 wt.% MNPs).Furthermore, the temperature dependence of the coercive field changes forall samples, suggesting a strong influence of the polymer properties onthe magnetic properties of the MNCPs.","palabras_clave":"MAGNETIC NANOCOMPOSITES|BIO-BASED POLYMERS|SAXS TECHNIQUE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4942,"idioma_id":4,"titulo":"Antimicrobial Activity of Starch Hydrogel Incorporated with Copper  Nanoparticles","resumen":"In order to obtain an antimicrobial gel, a starch-based hydrogel reinforced withsilica-coated copper nanoparticles (Cu NPs) was developed. Cu NPs were synthesized by use of a copper salt and hydrazine as a reducing agent. In order to enhance Cu NP stability over time, they were synthesized in a starch medium followed by a silica coating. The starch hydrogel was prepared by use of urea and water as plasticizers and it was treated with different concentrations of silica-coated copper nanoparticles (Si&#8722;Cu NPs). The obtained materials were characterized by Fourier transform infrared (FT-IR) spectroscopy, electron paramagnetic resonance (EPR) spectroscopy, scanning electron microscopy (SEM), and rheometry. FT-IR and EPR spectra were used for characterization of Cu NPs and Si&#8722;Cu NPs, confirming that a starch cap was formed around the Cu NP and demonstrating the stability of the copper nanoparticle after the silica coating step. SEM images showed Cu NP, Si&#8722;Cu NP, and hydrogel morphology. The particle size was polydisperse and the structure of the gels changed along with particle concentration. Increased NP content led to larger pores in starch structure. These results were in accordance with the rheological behavior, where reinforcement by the Si&#8722;Cu NP was seen. Antimicrobial activity was evaluated against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcusaureus) bacterial species. The hydrogels were demonstrated to maintain antimicrobial activity for at least four cycles of use. A dermal acute toxicity test showed that the material could be scored as slightly irritant, proving its biocompatibility. With these advantages, it is believed that the designed Si&#8722;Cu NP loaded hydrogel may show high potential for applications in various clinicalfields, such as wound dressings and fillers.","palabras_clave":"starch hydrogel|copper nanoparticles|antimicrobial material|reinforcement"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4943,"idioma_id":4,"titulo":"Physicochemical Properties and Proposed Mechanism in the Obtainment of 4-Hidroxycoumarin Conjugated Polymers Using MALDI-T of Analysis","resumen":"The mechanism  for  the poly-condensation event of conjugated polymers with ending 4-hydroxycoumarin has been proposed. It happened under H2SO4 acidic conditions only using enolic-coumarins without any substituent at  third position.  It was  studied using Matrix  assisted  laser Desorption\/ ionization  time-of-flight  (MALDI-TOF) mass spectrometry. Besides, some physicochemical properties were analyzed using Thermo-gravimetric (TGA), X-ray and UV-Vis analysis.","palabras_clave":"SELF-CONDENSATION MECHANISM|MALDI TOF|X RAY|TELECHELIC POLYMER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4944,"idioma_id":4,"titulo":"A differential bialgebra associated to a set theoretical solution of the Yang-Baxter equation","resumen":"For a set theoretical solution of the Yang-Baxter equation (X, &#963;), we define a d.g.bialgebra B = B(X, &#963;), containing the semigroup algebra A = k{X}\/ xy = zt :&#963;(x, y) = (z, t) , such that k &#8855;A B &#8855;A k and HomA&#8722;A (B, k) are respectively the homologyand cohomology complexes computing biquandle homology and cohomology defined in[CEGN, CJKS] and other generalizations of cohomology of rack-quanlde case (for ex-ample defined in [CES2]). This algebraic structure allow us to show the existence ofan associative product in the cohomology of biquandles, and a comparison map withHochschild (co)homology of the algebra A.","palabras_clave":"Yang Baxter equation|rack, quandles|biquandles biracks|cohomology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4945,"idioma_id":4,"titulo":"Iterated non-linear model predictive control based on tubes and contractive constraints","resumen":"This paper presents a predictive control algorithm for non-linear systems based on successive linearizations of the non-linear dynamic around a given trajectory. A linear time varying model is obtained and the non-convex constrained optimization problem is transformed into a sequence of locally convex ones. The robustness of the proposed algorithm is addressed adding a convex contractive constraint. To account for linearization errors and to obtain more accurate results an inner iteration loop is added to the algorithm. A simple methodology to obtain an outer bounding-tube for state trajectories is also presented. The convergence of the iterative process and the stability of the closed-loop system are analyzed. The simulation results show the effectiveness of the proposed algorithm in controlling a quadcopter type unmanned aerial vehicle.","palabras_clave":"CONTRACTIVE CONSTRAINT|ITERATIVE PROCESS|NON-LINEAR MODEL PREDICTIVE CONTROL|ROBUST MODEL PREDICTIVE CONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4946,"idioma_id":4,"titulo":"Arbuscular Mycorrhizal Pecan Seedlings Alleviate Effect of Restricted Water Supply","resumen":"Pecan [Carya illinoinensis (Wangenheim) K. Koch] seedlings were inoculated with the arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis to evaluate if these organisms establish a symbiotic association and if such an association exerts an influence on restricted water supply response by the plant. After fungal inoculation, plants were grown in a growth chamber. All plants were effectively mycorrhized, with an average infection of 32% for each root. Mycorrhizal colonization led to increased stem height in pecan plants. On restricted water supply, mycorrhizal pecan seedlings presented a higher number of lateral roots and more stem height and diameter than nonmycorrhizal ones. Besides, nonmycorrhizal plants showed an increase in proline content with regard to mycorrhizal ones. Depending on the results, pecan seedlings can be efficiently colonized with the mycorrhizal fungus R. irregularis, under controlled conditions proposed in this work, whereas the symbiotic association with the fungus mitigates restricted water supply.","palabras_clave":"CARYA ILLINOINENSIS |RHIZOPHAGUS IRREGULARIS|MYCORRHIZAL|LATERAL ROOT|PROLINE|STEM HEIGHT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4947,"idioma_id":4,"titulo":"Angular distribution of cosmic rays from an individual source in a turbulent magnetic field","resumen":"We obtain the angular distribution of the cosmic rays reaching an observer from an individual source and after propagation through a turbulent magnetic field, for different ratios between the source distance and the diffusion length. We study both the high-energy quasirectilinear regime as well as the transition towards the diffusive regime at lower energies where the deflections become large. We consider the impact of energy losses, showing that they tend to enhance the anisotropy of the source at a given energy. We also discuss lensing effects, in particular, those that could result from the regular Galactic magnetic field component and show that the effect of the turbulent extragalactic magnetic fields can smooth out the divergent magnification peaks that will result for pointlike sources in the limit of no turbulent deflections.","palabras_clave":"Ultra high energy cosmic rays|Anisotropy|Diffusion|Magnetic fields"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4948,"idioma_id":5,"titulo":"Efectos del disturbio antrópico sobre las poblaciones de  Leprolochus birabeni (Araneae, Zodariidae) en el Chaco Seco del noroeste de Argentina","resumen":"Little is known about the biology and ecology of the myrmecophagic species Leprolochus birabeni Mello-Leitão, 1942 (Zodariidae), the only representative of this genus in Argentina. This species is typical of arid environments of the north of Argentina, and their study in the Chaco Seco is relevant, because is a highly disturbed ecoregion and one of the few with dry subtropical forests in the World. The aim of this work is to study the demography of this species, considering its seasonal variation in native environments, and if the livestock grazing and the agriculture, two of the most common anthropogenic activities of the Chaco Seco ecoregion, produce changes in their populations in the province of Salta (Argentina). We consider three type of environments: native (NA); aff ected by cattle and goats (NP); and aff ected by agriculture (NC). The latter corresponds to patches of native vegetation in an agricultural matrix. Three sampling sites were selected on each type of environment, where seasonal samplings using pitfall traps were performed during autumn, winter and spring (2006) and summer (2007). We collected 549 spiders of L. birabeni, with the juveniles reaching until the 64.12% of total abundance, followed by males (26.41%) and females (9.47%). At landscape level, spring season showed the highest number of individuals, reaching 44.99% of the total abundance, while autumn reported the lowest percentage (7.83%). The juveniles increase observed during the winter would indicate an adaptation to environmental conditions. The same pattern was observed in NA and NC environments, but not in NP ones. Thelater environments showed a decrease in the adult abundances across the seasons, while the NC populations showed an increase of male abundances during spring season with a sharp reduction of females and juveniles throughout the year. The abundance of L. birabeni in NA environments was higher (41.06% of total abundance) and the least value was reported in NC environments (24.67%). The two most common anthropogenic activities affecting the Chaco Seco of the province of Salta modify both the seasonal changes as the representation of the diff erent developmental stages and sexes of thenative populations of L. birabeni, being the most damaging factor the modifi cation of the native environments by the agriculture.","palabras_clave":"Spiders|Demography|Seasonal variation|population changes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4950,"idioma_id":4,"titulo":"Voltage unbalance and harmonic distortion effects on induction motor power, torque and vibrations","resumen":"Voltage unbalance and harmonic voltage distortion affect the power, torque and vibrations on induction motors (IM). In this paper, we compute the frequency of the oscillations of the power, torque and vibrations due to voltage unbalance and harmonics and we present laboratory results to verify the calculations. We also show that typical values of harmonic distortion combined with the maximum levels of voltage unbalance allowed by standards are sufficient to introduce vibrations levels under which continuous operation of IMs is not recommended. This result has important implications for root-cause analysis of IM trip due to vibrations because protection engineers can rule out mechanical problems by verifying that vibrations are only present during high levels of voltage unbalance and harmonic distortion.","palabras_clave":"POWER QUALITY|HARMONIC DISTORTION|VOLTAGE UNBALANCE|VIBRATIONS|INDUCTION MOTORS|FAULT DIAGNOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4951,"idioma_id":4,"titulo":"Evidence for a Role of Glycoprotein M6a in Dendritic Spine Formation and Synaptogenesis","resumen":"Neuronal glycoprotein M6a belongs to the tetraspan proteolipidprotein (PLP) family. Mutations in GPM6A gene have been related to mentaldisorders like schizophrenia, bipolar disorders and claustrophobia. M6ais expressed mainly in neuronal cells of the central nervous system andit has been extensively related to neuronal plasticity. M6a inducesneuritogenesis and axon\/filopodium outgrowth; however its mechanism ofaction is still unresolved. We recently reported that the integrity ofthe transmembrane domains (TMDs) 2 and 4 are critical for M6a filopodiainduction. There is also experimental data suggesting that M6a might beinvolved in synaptogenesis. In this regard, we have previously determinedthat M6a is involved in filopodia motility, a process that is describedin the first step of the filopodial model for synaptogenesis. In thiswork we analyzed the possible involvement of M6a in synaptogenesis andspinogenesis, and evaluated the effect of two non-synonymous SNPs presentin the coding region of TMD2-GPM6A in these processes. The results showedthat endogenous M6a colocalized with both, pre-synaptic (synaptophysin)and post-synaptic (NMDA-R1), markers along of neuronal soma anddendrites. M6a-overexpressing neurons displayed an increased number ofsynaptophysin and NMDA-R1 puncta and, also, an increased number ofcolocalization puncta between both markers. Conversely, the number ofsynaptic puncta markers in neurons expressing nsSNP variants was similarto those of control neurons. Expression of M6a is accompanied by anincrement in spine density, particularly in mature spines, as comparedwith neurons expressing mGFP or GPM6A nsSNP variants. Taken together,these results suggest that M6a contributes positively to spine and,likely, synapse formation","palabras_clave":"PLP family|Transmembrane domains|Synapses|Dendritic arborization| nsSNPs variants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4952,"idioma_id":4,"titulo":"Current-sensor fault detection and isolation for induction-motor drives using a geometric approach","resumen":"This work presents the design of a current-sensor fault detection and isolation system for induction-motor drives. A differential geometric approach is addressed to determine if faults can be detected and isolated in drives with two line current sensors by using a model based strategy. A set of subsystems is obtained based on the observability co-distribution, whose outputs are decoupled from the load torque (detectability) and only affected by one of the sensors (isolability). A bank of observers is designed for these subsystems in order to obtain residuals for the fault detection and isolation. It is demonstrated that the proposed strategy allows detecting single and multiple sensor faults, including disconnection, offset and gain faults. Experimental results validate the proposal.","palabras_clave":"induction motor drive|fault detection and isolation|nonlinear observer|sensor|geometric approach"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4953,"idioma_id":5,"titulo":"ANALISIS ORGANIZACIONAL DEL INSTITUTO TECNOLOGICO","resumen":"Se llevó adelante un análisis organizacional de una institución dedicada a la enseñanza tec-nológica de la provincia de Buenos Aires, República Argentina. La perspectiva desarrollada se enmarca en la propuesta de análisis organizacional de Krieger (2015) donde se plantea el debate de sociología de las organizaciones en un campo de estudio interdisciplinar. Se analizaron el estrés laboral, la motivación, el funcionamiento en equipo, medioambiente, ética organizacional, comunicación, cultura, liderazgo y la tecnología. Se evidencia una organización con pocos años de funcionamiento, que propicia el clima institucional acorde a sus objetivos y a las posibilidades de sus miembros, con un nivel bajo de violencia y conflictividad, con un cum-plimiento de los objetivos institucionales. Las autoridades entrevistadas no visualizan conflictos y muestran un desempeño en una zona de confort, ligada a la primarización de los vínculos.","palabras_clave":"Sociología de las organizaciones|Instituciones|Interdisciplinar |Instituto tecnológico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4954,"idioma_id":4,"titulo":"The Knowns Unknowns: Exploring the Homologous Recombination Repair Pathway in Toxoplasma gondii","resumen":"Toxoplasma gondiiis an apicomplexan parasite of medical and veterinary importance which causes toxoplasmosisin humans. Great effort is currently being devoted towards the identificationof novel drugs capable of targeting such illness. In this context, we believethat the thorough understanding of the life cycle of this model parasite will facilitatethe identification of new druggable targets in T. gondii. It is important to exploit the available knowledge of pathwayswhich could modulate the sensitivity of the parasite to DNA damaging agents.The homologous recombination repair (HRR) pathway may be of particular interestin this regard as its inactivation sensitizes other cellular models such ashuman cancer to targeted therapy. Herein we discuss the information availableon T. gondii´s HRR pathway from theperspective of its conservation with respect to yeast and humans. Specialattention was devoted to BRCT domain-containing and end-resection associated proteinsin T. gondii as in other experimentalmodels such proteins have crucial roles in early\/late steps or HRR and in the pathwaychoice for double strand break resolution.We conclude that T. gondii HRR pathway is a source of several lines of investigationthat allow to &nbsp;to comprehend the extentof diversification of HRR in T. gondii.Such an effort will serve to determine if HRR could represent a potentialtarger for the treatment of toxoplasmosis. ","palabras_clave":"Toxoplasma|recombinación homóloga|daño de ADN|replicación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4955,"idioma_id":4,"titulo":"Agricultural impact assessment and management after three widespread tephra falls in Patagonia, South America","resumen":"Agriculturalproduction is often concentrated in volcanically active or previously activeareas where weathered volcanic products form fertile soils. However, this proximitymeans agriculture is exposed to tephra fall hazards. The type and severity of impactsto agricultural systems from tephra fall are dependent on both the hazardintensity metrics (tephra fall characteristics, such as thickness, grain size)and the vulnerability characteristics of the exposed agricultural system(s).Understanding the relationship between significant intensity metrics of tephrafall hazard and farm-scale and region-scale vulnerabilities is key to impactassessment and informing management and recovery strategies. Several largesilicic eruptions have occurred over the past 20 years in the Patagonian regionof South America, including the 1991 Hudson, 2008 Chaiten, and 2011 CordonCaulle eruptions. These events deposited varying thicknesses of tephra onthousands of farms distributed across a variety of climates and productionstyles. Drawing on impact assessment data collected from interviews undertakenon post-event impact assessment reconnaissance trips, and other reports, thisstudy evaluates the importance of tephra thickness as a hazard intensitymetric, and vulnerability characteristics, when assessing impacts in the shortand long term and, compares the effectiveness of response and recoverystrategies. Whilst tephra thickness was the best single indicator ofagricultural production losses, other factors, notably climate, farm type, andaccess to mitigation measures such as irrigation and\/or cultivation, were alsoimportant indicators of damage. The climatic zone and associated precipitationlevel was found to be one of the most important characteristics ofvulnerability, with higher damage occurring at lower tephra thicknesses in thesemi-arid regions compared to farms in the temperate zone.","palabras_clave":"TEPHRA FALL|AGRICULTURE |IMPACT ASSESSMENT |CORDÓN CAULLE-VOLCANIC COMPLEX|CHAITÉN 2008 ERUPTION|HUDSON 1991 ERUPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4956,"idioma_id":4,"titulo":"Impacts to agriculture and critical infrastructure in Argentina after ashfall from the 2011 eruption of the Cordón Caulle volcanic complex: an assessment of published damage and function thresholds","resumen":"The 2011 Cordón Caulle (Chile) was a large silicic eruption that dispersed ashfall over 75,000 km2 of land in Central Argentina, affecting large parts of the Neuquén, Río Negro, and Chubut provinces, including the urban areas of Villa la Angostura, Bariloche and Jacobacci. These regions all received damage and disruption to critical infrastructure and agriculture due to the ashfall. We describe these impacts and classify them according to published damage\/disruption states (DDS). DDS for infrastructure and agriculture were also assigned to each area using the tephra thickness thresholds suggested by previous studies reported in the volcanological literature. The objective of this study was to evaluate whether the impacts were as expected based on the DDS suggested thresholds, and to determine whether other factors, apart from ashfall thickness, played a part. DDS thresholds based on tephra thickness were a good predictor of the impacts that occurred in the semi-arid steppe area around Jacobacci. This was unexpected as the more severe impacts were related to the challenging environmental conditions (low precipitation levels, high levels of wind erosion) and the daily wind remobilisation of ash that occurred, rather than the ashfall thicknesses received. The temperate region, including Villa la Angostura and Bariloche, performed better than the DDS assigned by ashfall thickness suggested. Despite deposits as thick as 300 mm, full recovery occurred within months of the ashfall event. The DDS scales need to incorporate a wider range of system characteristics, and environmental and vulnerability factors, as we propose here.","palabras_clave":"VOLCANIC ASH|PUYEHUE CORDON CAULLE|ASHFALL IMPACTS|TEPHRA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4957,"idioma_id":4,"titulo":"Subaqueous landslides at the distal basin of Lago Nahuel Huapi (Argentina): Towards a tsunami hazard evaluation in Northern Patagonian lakes","resumen":"TheMay 22nd, 1960 Valdivia earthquake, Chile (Mw9.5) triggered a series of subaqueous mass-wasting processes (debris flows and slides) in Lago Nahuel Huapi (Argentina), generating a tsunami-like wave that hit the coasts of San Carlos de Bariloche. Aiming to provide a first preliminary insight into tsunami hazards for the lakeshore communities, in this paper we identify and characterize the subaqueous landslides at the populated distal basin of the lake. Swath bathymetric and seismic profiling surveys were carried out and high-resolution digital elevation models were derived fromthese data to perform a landslide inventory map. A series of morphometricalparameters (including the landslide area, the volume of displaced materials and the run-out distance, among others) were estimated upon selected events. The results indicated that landslide activity at the distal basin of Lago Nahuel Huapi has been concentrated in the vicinity of Bariloche (massive landslide triggered by the 1960 earthquake) and within steep delta fronts where the slope failures typically initiate at shallow waters (9-11 m depth). The sliding mass frequently travels basinward along a great distance (&#8805;1000 m). At the delta fronts, the volume of material removed by landslides can reach ~40 × 104 m3, leaving scar areas of up to 13 m thick. The periodic occurrence of rotational?translational mass movements initiating at the upper edge of the deltafronts, with vertical displacements of the mobilized materials reaching ~200 m, probably represents a potential tsunami hazard for the nearby populated coasts.","palabras_clave":"NAHUEL HUAPI|SUBAQUEOUS LANDSLIDES|LACUSTRINE DELTAS|TSUNAMI HAZARD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4958,"idioma_id":4,"titulo":"GBStools: A Statistical Method for Estimating Allelic Dropout in Reduced Representation Sequencing Data","resumen":"Reduced representation sequencing methods such as genotyping-by- Sequencing (GBS)enable low-cost measurement of genetic variation without the need for a reference genome assembly. These methods are widely used in genetic mapping and population genetics studies, especially with non-model organisms. Variant calling error rates, however, are higher in GBS than in standard sequencing, in particular due to restriction site polymorphisms,and few computational tools exist that specifically model and correct these errors. We developed a statistical method to remove errors caused by restriction site polymorphisms,implemented in the software package GBStools. We evaluated it in several simulateddata sets, varying in number of samples, mean coverage and population mutation rate, and in two empirical human data sets (N = 8 and N = 63 samples). In our simulations, GBStools improved genotype accuracy more than commonly used filters such as Hardy-Weinberg equilibrium p-values. GBStools is most effective at removing genotype errors in data sets over 100 samples when coverage is 40X or higher, and the improvement is mostpronounced in species with high genomic diversity. We also demonstrate the utility of GBS and GBStools for human population genetic inference in Argentine populations and reveal widely varying individual ancestry proportions and an excess of singletons, consistent with recent population growth.","palabras_clave":"Genomics|Argentina |1000 Genomes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4959,"idioma_id":4,"titulo":"Pleistocene climate changes affected species ranges and evolutionary processes in the Atlantic Forest","resumen":"The effects of global glaciations on the distribution of organisms is an essential element of many diversification models. However, the empirical evidence supporting this idea is mixed, in particular with respect to explaining tropical forest evolution. In the present study, we evaluated the impacts of range shifts associated with Pleistocene global glacial cycles on the evolution of tropical forests. In particular, we tested the predictions: (1) that population genetic structure increases with fragmentation variation between the present and the Last Glacial Maximum (LGM) and also (2) with geographical range instability; and (3) that genetic diversity increases with range stability and (4) decreases with fragmentation variation between periods. To address our predictions, we studied population genetic structures and modelled present and past distributions of 15 Atlantic Forest (AF) endemic birds. Afterwards, we evaluated the relationship of population genetic parameters with metrics of species range shifts between the present and the LGM. We found that geographical ranges of AF birds changed in concert with Pleistocene glacial cycles but, unexpectedly, our findings suggest the novel idea that ranges during glacial maxima were slightly larger on average, as well as equally fragmented and displaced from the interglacial ranges. Our findings suggest that range shifts over the late Pleistocene impacted on the diversification of forest organisms, although they did not show that those range shifts had a strong effect. We found that a combination of fragmentation variation across time, small current range size, and range stability increased population genetic structure. However, neither fragmentation, nor range stability affected genetic diversity. Our study showed that evolutionary responses to range shifts across AF birds have a high variance, which could explain the mixed support given by single-species studies to the action of Pleistocene range shifts on population evolution. © 2016 The Linnean Society of London, Biological Journal of the Linnean Society, 2016, 00, 000?000.","palabras_clave":"Atlantic Forest|birds|population genetic structure|Pleistocene glaciations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4960,"idioma_id":4,"titulo":"The expansion of adult stem\/progenitor cells and their marker expression fluctuations are linked with pituitary plastic adaptation during gestation and lactancy","resumen":"Extensiveevidence has revealed variations in the number of hormone-producing cells inthe pituitary gland, which occur under physiological conditions such asgestation and lactancy. It has been proposed that new hormone producing cellsdifferentiate from stem cells. However, exactly how and when this takes placeis not clear. In this work, we used immunoelectron-microscopy to identify adultpituitary stem\/progenitor cells (SC\/P) localized in the marginal zone (MZ) andadditionally, we detected GFRa2, Sox2 and Sox9 positive cells in theadenoparenchyma (AP) by fluorescence microscopy. Then, we evaluatedfluctuations of SC\/P mRNA and protein level markers in MZ and AP duringgestation and lactancy. An up-regulation in stemness markers was shown at termof gestation (AT) in MZ, while, there was more progenitor cell marker at middleof gestation and active lactancy. Concerning committed cell marker, we detecteda rise in AP at beginning of lactancy (d1L). We performed a BrdU-uptakeanalysis in MZ and AP cells. The highest level of BrdU-uptake was observed inMZ AT cells whereas, in AP, this was detected in d1L, followed by a decrease inboth the MZ and AP. Finally, we detected double-immunostaining for BrdU-GFRa2in MZ AT cells and BrdU-Sox9 in the AP d1L cells. Taken together, wehypothesize that the expansion of the SC\/P niche took place mainly in MZ frompituitary rats in AT and d1L. These results suggest that the SC niche activelyparticipates in pituitary plasticity during these reproductive states, contributingto the origin of hormone cell populations.","palabras_clave":"Pituitary gland|Stem cell\/progenitor markers|Marginal zone|Gestation and Lactancy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4961,"idioma_id":4,"titulo":"Mechanics of polycarbonate in biaxial impact loading","resumen":"We investigate the deformation and failure behavior of Polycarbonate (PC) under low-velocity biaxial impact loading. For this, we have conducted experiments and numerical simulations of the falling weight impact test (FWT) for different testing configurations. To model PC behavior in dynamic situations, we employed a 3D thermomechanical constitutive model that captures the strain rate, pressure and temperature dependence of deformation response. The finite element model also incorporates adiabatic heating and contact friction effects. Numerical predictions of the FWT force?displacement curves are in good agreement with experimental observations. An assessment of the developed stress?strain fields indicates that the effective plastic stretch &#955;p is the invariant that most suitably represents the point of material failure in all different test configurations. We examine the relation of a maximum &#955;p fracture criterion with existing criteria for PC on the grounds of the triaxiality conditions specific to the biaxial impact test. Finally, we propose an extension of these criteria to enhance their capability of predicting failure in part geometries that may become subjected to more complex multiaxial loading scenarios.","palabras_clave":"Biaxial impact|Polycarbonate|Constitutive modeling|Finite element analysis|Failure criteria"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4962,"idioma_id":4,"titulo":"Genome-Wide Analyses of Subtilisin-Like Serine Proteases on Solanum tuberosum","resumen":"Las serin proteasas tipo subtilisinas o subtilasas constituyen una gran subfamilia de genes en papa. En este trabajo se identificaron 82 subtilasas codificadas por 74 genes. Todas las subtilasas presentan un dominio S08 característico, y la mayoría de ellas poseen un dominio asociado a proteasa, un dominio inhibidor I9 y un péptido señal que consiste en una señal que conduce a la vía secretora. En los estudios filogenéticos se describen cinco grupos de subtilasas nombrados de StSBT1 a StSBT5. La expansión en la subfamilia de subtilasas se ve reflejada en dos grupos,  StSBT1 y StSBT4, caracterizados por la presencia de genes sin intrones con disposición en tándem localizados en el cromosoma I y VIII. Veintinueve de las subtilasas identificadas co-localizan con seis de los 24 metaQTLs de resistencia a tizón tardío previamente descriptos en papa. Estos metaQTLs incluyen genes de subtilasas con regulación positiva en ensayos de hojas de papa desprendidas inoculadas con P. infestans, algunos de los cuáles son  homologos a genes de subtilasas p69 descriptos en tomate.","palabras_clave":"Subtilases |Potato |Phytophthora_infestans Meta QTLs|intronless genes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4963,"idioma_id":4,"titulo":"Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability","resumen":"Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.","palabras_clave":"WHITE ROT FUNGI|TRAMETES|LACCASE|MANGANESE PEROXIDASE|CU+2 RESPONSE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4964,"idioma_id":4,"titulo":"Genome analysis of a clinical isolate of Shewanella sp. uncovered an active hybrid integrative and conjugative element (ICE) carrying an integron platform inserted in a novel genomic locus","resumen":"Shewanella spp. are currently considered emerging pathogens that can code for a blaOXA carbapenemase in their chromosome. Complete genome analysis of the clinical isolate Shewanella sp. Sh95 revealed that this strain is a novel species that shares a lineage with marine isolates. Characterization of its resistome showed that it codes for genes drfA15, qacH and blaOXA-48. We propose that Shewanella sp. Sh95 acts as reservoir of blaOXA-48. Moreover, mobilome analysis showed that it contains a novel ICE, named ICESh95. Comparative analysis between the close relatives ICESpuPO1 from Shewanella sp. W3-18-1 and ICE SXTMO10 from Vibrio cholerae showed that ICESh95 encompassed two new regions, a type III restriction modification system and a multidrug resistance integron. The integron platform contained a novel arrangement formed by gene cassettes drfA15 and qacH and a class C-attC group II intron. Furthermore, insertion of ICESh95 occurred at a unique target site, which correlates with the presence of a different xis\/int module. Mobility of ICESh95 was assessed showing its ability to self-transfer at high levels to different bacteria species. Our results show that ICESh95 is a self-transmissible mobile element that can contribute to the dissemination of antimicrobial resistance, which is a clear threat when natural bacteria from water ecosystems, such as Shewanella, act as vector in its propagation.","palabras_clave":"Shewanella spp. |Integrative and conjugative element (ICE) |blaOXA carbapenemase|horizontal lateral transfer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4965,"idioma_id":4,"titulo":"GH10 XynA is the main xylanase identified in the crude enzymatic extract of Paenibacillus sp. A59 when grown on xylan or lignocellulosic biomass","resumen":"A novel bacterial isolate with polysaccharides degrading activity was identified as Paenibacillus sp., andnamed Paenibacillus sp. A59. Even though it is a strict mesophile, optimal xylanase activity of the crudeenzymatic extract was achieved between 50&#9702;C and 70&#9702;C and more than 60% of the activity was retainedafter incubation for 48 h at 50&#9702;C, indicating thermotolerance of the enzymes involved. The extract wasalso active on pre-treated sugarcane residue (SCR) and wheat straw, releasing xylobiose and xylose asthe main products, therefore confirming its predominantly xylanolytic activity. By zymograms and massspectrometry of crude enzymatic extracts of xylan or SCR cultures, a 32 kDa GH10 beta- 1,4- endoxylanasewith xylanase and no CMCase activity was identified. We named this enzyme XynA and it was the onlyxylanase identified under both conditions assayed, suggesting that it is a good candidate for recombinantexpression and evaluation in hemicelluloses deconstruction applications. Also, a protein with two S-layerhomology domains (SLH) and a large uncharacterized C-terminal domain as well as an ABC substratebinding protein were identified in crude extracts of SCR cultures. We propose that Paenibacillus sp. A59uses a system similar to anaerobic and other Gram positive bacteria, with SLH-domain proteins anchoringpolysaccharide-degrading enzymes close to the membrane and the substrate binding protein assistingtranslocation of simple sugars to the cell interior.","palabras_clave":"Paenibacillus|Xylanase|GH10|SLH domain|Lignocellulosic biomass"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4966,"idioma_id":4,"titulo":"A Fishtail Projectile Point from the Southern Pampean Hills, Characato, Córdoba, Argentina","resumen":"Here we report a newly discovered Fishtail projectile point, or Fell 1 projectile-point type, from Characato valley in the southern Pampean Hills of central Argentina. It is a fluted basal stem fragment on a non-local dark red volcanic rock. Fishtail points are rare, and archaeological sites dating to the Pleistocene-Holocene transition particularly scarce, in this region; hence, this new evidence importantly establishes early human occupation through material culture.","palabras_clave":"Fishtail projectile point|Córdoba|Argentina|Surface"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4967,"idioma_id":4,"titulo":"Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion","resumen":"Amaranth protein isolate was obtained and subjected to simulated gastrointestinal digestion to evaluate its potential antithrombotic activity. The protein isolate did not present fibrin clotting inhibition at the concentrations studied, whereas the hydrolysate (DH%&#8201;=&#8201;51.1&#8201;±&#8201;3.8%) exhibited inhibition of fibrin coagulation, showing a dose?response behaviour (IC50&#8201;=&#8201;0.23&#8201;±&#8201;0.02&#8201;mg\/mL), confirming that the enzymatic treatment was able to release bioactive peptides from amaranth proteins. A fraction with high antithrombotic activity was obtained from this hydrolysate, and resulted to be three times more potent than its original sample (IC50&#8201;=&#8201;0.07&#8201;±&#8201;0.01&#8201;mg\/mL). The absorption of this active fraction was studied with an in vitro peptides transport assay through intestinal epithelium and it was observed that some peptides are able to cross the Caco2-TC7 cell monolayer. Potentially bioactive peptides were found after sequencing them, and informatics tools allowed us to select and locate in their native molecules those peptides prone to inhibit thrombin activity.","palabras_clave":"AMARANTH PROTEIN ISOLATE |GASTROINTESTINAL DIGESTION |ANTITHROMBOTIC ACTIVITY |ABSORPTION BY CACO 2 CELL CULTURE |BIOACTIVE PEPTIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4968,"idioma_id":4,"titulo":"Macroparasites of silversides (Atherinopsidae, Odontesthes) in Argentina","resumen":"This study presents new geographical distribution records for the macroparasites of the marine Odontesthes nigricans (Richardson, 1848) (89wild specimens), and for the freshwater silversides Odontesthes bonariensis (Valenciennes, 1835) (43 wild and 108 cultured specimens), andOdontesthes hatcheri (Eigenmann, 1909) (183 wild specimens) from Argentina. These data represent records of 12 parasite taxa for O. nigricans,8 for O. bonariensis, and 19 for O. hatcheri, which include digeneans, monogeneans, cestodes, nematodes, acantocephalans, mollusks, copepods,and branchiurans. For cultured O. bonariensis, the records of the present study correspond to the first reports of parasites in the literature. Thiswork provides also data on site of infection, parasite load, and development stages for the parasite species.All Rights Reserved © 2016 Universidad Nacional Autónoma de México, Instituto de Biología. This is an open access item distributed under theCreative Commons CC License BY-NC-ND 4.0.","palabras_clave":"Odontesthes nigricans|Odontesthes bonariensis|Odontesthes hatcheri|parasites"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4970,"idioma_id":4,"titulo":"Prenatal hyperandrogenism induces alterations that affect liver lipid metabolism","resumen":"AbstractPrenatal hyperandrogenism is hypothesized as one of the main factors contributingto the development of polycystic ovary syndrome (PCOS). PCOS patients have highrisk of developing fatty liver and steatosis. This study aimed to evaluate the role ofprenatal hyperandrogenism in liver lipid metabolism and fatty liver development.Pregnant rats were hyperandrogenized with testosterone. At pubertal age, theprenatally hyperandrogenized (PH) female offspring displayed both ovulatory (PHov)and anovulatory (PHanov) phenotypes that mimic human PCOS features. We evaluatedhepatic transferases, liver lipid content, the balance between lipogenesis and fatty acidoxidation pathway, oxidant\/antioxidant balance and proinflammatory status. We alsoevaluated the general metabolic status through growth rate curve, basal glucose andinsulin levels, glucose tolerance test, HOMA-IR index and serum lipid profile. Althoughneither PH group showed signs of liver lipid content, the lipogenesis and fatty oxidationpathways were altered. The PH groups also showed impaired oxidant\/antioxidantbalance, a decrease in the proinflammatory pathway (measured by prostaglandin E2 andcyclooxygenase-2 levels), decreased glucose tolerance, imbalance of circulating lipidsand increased risk of metabolic syndrome. We conclude that prenatal hyperandrogenismgenerates both PHov and PHanov phenotypes with signs of liver alterations, imbalancein lipid metabolism and increased risk of developing metabolic syndrome. Theanovulatory phenotype showed more alterations in liver lipogenesis and a moreimpaired balance of insulin and glucose metabolism, being more susceptible to thedevelopment of steatosis.","palabras_clave":"POLYCYSTIC OVARY SYNDROME  |FATTY LIVER|PRENATAL HYPERANDROGENISM |LIPID METABOLISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4971,"idioma_id":4,"titulo":"Gelatin based films capable of modifying its color against environmental pH changes","resumen":"The aim of this work was to develop biodegradable protein-based films capable of sense pH changes. These protein films were prepared by casting from aqueous solutions of bovine gelatin, glycerol and three acid-base indicators: methyl orange (MO), neutral red (NR) and bromocresol green (BCG), at pH 2, 6 and 11. All resulting protein films were homogeneous, thin and had different colors depending on pH and the indicator used. The response of these materials was evaluated simulating their contact with liquid and semisolid media, and with a container headspace at acid and alkaline pH. In all tests, developed protein films could modify their color after being in contact with media of different pH. The physicochemical properties of films were also affected differently by the presence of each acid-base indicator. While the addition of BCG did not significantly modify the properties of control gelatin films, except its color; the incorporation of MO and NR into film-forming solutions significantly improved mechanical properties and decreased the water solubility and moisture content of the resulting protein films without affecting their water vapor permeability.","palabras_clave":"Smart packaging|Protein film|pH indicators|Gelatin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4972,"idioma_id":4,"titulo":"Was there a Process of Regionalization in Northeastern Patagonia during the Late Holocene?","resumen":"The objective of this article is to evaluate if changes in the social organization of hunter-gatherer groups from northeastern Patagonia (Argentina)during the Final Late Holocene (ca. 1000?250 years BP) maybe understood as a regionalization process. A portion of this region,the lower course of the Colorado River, is taken as a case study. Thisarid\/semi-arid area is part of an ecotone between the Pampas and northeastern Patagonia situated beside the Atlantic coast. Most of theevidence for occupation is concentrated in the Late Holocene, more precisely from 1000 to 250 BP. The archaeological record of this periodindicates increased human population, along with a greater intensityof site occupation, the first systematic evidence of coastal occupation, reduced mobility and territoriality, diversification and intensification of subsistence practices, changes in the procurement and use of exotic raw materials, more complex funerary practices, and decreased circulationof specific images in portable artifacts. Based on these data, it is proposed that in some sectors of northeastern Patagonia during the Initial Late Holocene (3000?1000 BP) relatively open social networks operated,while for the Final Late Holocene (1000?250 BP) relatively closed social networks took their place within a framework of increasing regionalization. Factors relating to the latter are likely to have included demographic packing, spatial circumscription, territorial behaviors, and changes in population dynamics that were accompanied by social differentiation and more intense and competitive social interaction networks.","palabras_clave":"hunter gatherer groups|Late Holocene|social differentiation|Northeastern Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4973,"idioma_id":5,"titulo":"Estimación de la biomasa fúngica en un suelo del sudoeste de la provincia de Buenos Aires (Argentina) con una tinción directa con blanco de calcoflúor","resumen":"Los microorganismos del suelo son vitales para el correcto funcionamiento de los ecosistemas, principalmente por su rol en el ciclado de nutrientes. La intensificación del uso del suelo y las prácticas agrícolas alteran negativamente la actividad microbiana. La biomasa fúngica es uno de los parámetros más utilizados para estudiar el impacto de las actividades agrícolas en la estructura y funcionamiento del suelo. El objetivo del presente trabajo fue estimar la biomasa fúngica en un suelo del sudoeste bonaerense con el fin de obtener valores de referencia que permitan usar este parámetro como un indicador de cambios en el ecosistema y por otro lado, demostrar que la metodología empleada es sensible a las variaciones en las condiciones climáticas. Se colectaron muestras de suelos durante dos años consecutivos. Se prepararon frotis de suelo y se tiñeron con soluciones de distintas concentraciones de blanco de calcoflúor y luego se estimó la biomasa fúngica observando los frotis con microscopio de epifluorescencia. Los valores de biomasa fúngica estimados variaron entre 2,23 y 26,89 µg Cfúngico\/g de suelo y estuvieron dentro del rango esperable para el tipo de suelo estudiado. La biomasa fúngica mostró una relación positiva con la temperatura y las precipitaciones. La metodología empleada resultó ser confiable, repetible, y sensible a cambios en las condiciones climáticas. Los resultados podrían usarse como valores de referencia para estudiar en la biomasa fúngica de suelos bajo distintas condiciones y emplearse como indicadores del impacto de las distintas prácticas agrícolas sobre el ecosistema.","palabras_clave":"Biomasa |Hongos|Calcofluor|Suelo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4974,"idioma_id":5,"titulo":"BANCO DE SEMILLAS DEL SUELO EN UN ÁREA DE BOSQUES NATIVOS SUJETA A CAMBIO  EN EL USO DE LA TIERRA (ENTRE RIOS, ARGENTINA)","resumen":"El objetivo es analizar el banco de semillas del suelo (BSS) en un área de bosques nativos sujetos un cambio de uso de la tierra, y evaluar su rol en la regeneración de estos ecosistemas. Se evaluaron cuatro tratamientos: bosque nativo (BN), bosque nativo con manejo ganadero (BNM), bosque en Regeneración (BR) y agricultura (AGR). La mayor densidad del BSS (11.917 semillas\/m2) se registra en BNM, correspondiendo a un AGR el menor valor (3.142 semillas\/m2). Vachellia Caven resultó La única especie arbórea representada en el banco, con densidades relativas inferiores al 1%. Las especies invasoras dominaron en todos los tratamientos, a excepción del BNM, que presentó mayor Contribución de las gramíneas forrajeras. El 85% de las malezas determinadas en AGR fueron registrados-das en los otros tratamientos. El alto grado de fragmentación de los bosques nativos de la Cuenca,asociado al avance de la frontera agrícola, facilitaría el Flujo de semillas de especies invasoras desde áreas agrícolas hacia los bosques circundantes, constituyendo otro de los impactos negativos de la deforestación. (","palabras_clave":"AGROECOSISTEMAS BOSCOSOS|REGENERACION|RESERVORIO DE PROPAGULOS|SUCESION VEGETAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4975,"idioma_id":4,"titulo":"Early LPS-induced ERK activation in retinal pigment epithelium cells is dependent on PIP2-PLC","resumen":"This article presents additional data regarding the study ?The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium? [1]. The new data presented here show that short exposure of RPE cells to lipopolysaccharide (LPS) induces an early and transient activation of the extracellular signal-regulated kinase (ERK1\/2). This early ERK1\/2 activation is dependent on phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC). On the contrary, neither the phospholipase D 1 (PLD1) nor the PLD2 inhibition is able to modulate the early ERK1\/2 activation induced by LPS in RPE cells.","palabras_clave":"RETINAL PIGMENT EPITHELIUM|INFLAMMATION|PIP2-PLC|PLD|ERK|LPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4976,"idioma_id":4,"titulo":"Factors that influence the beta-diversity of spider communities in northwestern Argentinean Grasslands","resumen":"Beta-diversity, defined as spatial replacement in species composition, is crucial to the understanding of how local communities assemble. These changes can be driven by environmental or geographic factors (such as geographic distance), or a combination of the two. Spiders have been shown to be good indicators of environmental quality. Accordingly, spiders are used in this work as model taxa to establish whether there is a decrease in community similarity that corresponds to geographic distance in the grasslands of the Campos & Malezales ecoregion (Corrientes). Furthermore, the influence of climactic factors and local vegetation heterogeneity (environmental factors) on assemblage composition was evaluated. Finally, this study evaluated whether the differential dispersal capacity of spider families is a factor that influences their community structure at a regional scale. Spiders were collected with a G-Vac from vegetation in six grassland sites in the Campos & Malezales ecoregion that were separated by a minimum of 13 km. With this data, the impact of alpha-diversity and different environmental variables on the beta-diversity of spider communities was analysed. Likewise, the importance of species replacement and nesting on betadiversity and their contribution to the regional diversity of spider families with different dispersion capacities was evaluated. The regional and site-specific inventories obtained were complete. The similarity between spider communities declined as the geographic distance between sites increased. Environmental variables also influenced communitycomposition; stochastic events and abiotic forces were the principal intervening factors in assembly structure. The differential dispersal capacity of spider groups also influenced community structure at a regional scale. The regional beta-diversity, as well as species replacement, was greater in high and intermediate vagility spiders; while nesting was greater in spiders with low dispersion capacity. Geographic distance, among other factors (climate, and active and passive dispersion capacity), explains assembly structure and the decrease spider community similarity between geographically distant sites. Spiders with the highest dispersal capacity showed greater species replacement. This may be due to the discontinuity (both natural and anthropic) of the grasslands in this ecoregion, which limits the dispersal capacity of these spiders, and their close dependence on microhabitats. The dispersal capacity of the least vagile spiders is limited by geographic distance and biotic factors, such as competition, which could explain the nesting observed between their communities.","palabras_clave":"GEOGRAPHIC DISTANCE|ENVIRONMENTAL HETEROGENEITY|CLIMATE|CORRIENTES-ARGENTINA|DISPERSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4977,"idioma_id":4,"titulo":"Predators of bird nests in the  Atlantic forest of Argentina and Paraguay","resumen":"ABSTRACT.?Predation is the major cause of avian nest failure, and an important source of natural selection on life history traits and reproductive behavior. However, little is known about the identity of nest predators in much of the world, including the Neotropics. To identify some of the nest predators exerting selection pressure on birds of the subtropical Atlantic forest, we present observations of animals depredating bird nests in Argentina and Paraguay. We recorded depredations (destruction or removal of eggs or nestlings) at 33 nests of 25 species of birds, confirming as predators ten species of birds (Squirrel Cuckoo Piaya cayana, White-eared Puffbird Nystalus chacuru, Toco Toucan Ramphastos toco, Red-breasted Toucan Ramphastos dicolorus, Saffron Toucanet Pteroglossus baillonii, Chestnut-eared Aracari Pteroglossus castanotis, Planalto Woodcreeper Dendrocolaptes platyrostris, White-throated Woodcreeper Xiphocolaptes albicollis, Buff-browed Foliage-gleaner Syndactyla rufosuperciliata, and Plush-crested Jay Cyanocorax chrysops) and two species of medium-sized mammals (White-eared Opossum Didelphis albiventris and Crab-eating Fox Cerdocyon thous), and inferring two additional mammal species (Black Capuchin Monkey Sapajus nigritus and Southern Tigrina Leopardus guttulus). Fifty-five percent of these nests were depredated by toucans or aracaris (Ramphastidae), which destroyed eggs and nestlings at cup-, closed- and cavity-nests. Red-breasted Toucans destroyed nests 1.6?22 m high, in habitats ranging from primary forest to a backyard. Mammals and jays depredated nests from ground-level to midstory, whereas woodcreepers and aracaris depredated nests from the midstory to canopy. We did not record snakes at any bird nests, in strong contrast to studies from other Neotropical forests. Further studies should examine trade-offs among nest concealment, physical protection, and parental defense behavior as means of reducing nest predation, and use camera traps to quantify nest predation rates by predator species.","palabras_clave":"Atlantic forest|Neotropical|Nest fate|Nest predator|nest site|nest usurpation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4978,"idioma_id":4,"titulo":"Dissociative Adsorption of H2 on PtRu Bimetallic Surfaces","resumen":"We present a theoretical study of the dissociative adsorption of molecular hydrogen on PtRu bimetallic surfaces based on density functional theory (DFT) calculations. We focus on the reactivity of a pseudomorphic Pt monolayer deposited on Ru(0001), Pt1ML\/Ru(0001), for which we have obtained a minimum activation energy barrier for H2 dissociation, Eb = 0.32 eV, i.e., &#8764;0.3 and 0.26 eV higher than on Ru(0001) and Pt(111), respectively. Accordingly, the initial sticking probability for low energy impinging molecules (Ei &#8818; 0.1 eV) derived from classical trajectory calculations is various orders of magnitude smaller than on Ru(0001) in apparent contradiction with available experimental data. However, undercoordinated Pt atoms in the borders of Pt pseudomorphic islands and isolated Ru atoms or small Ru aggregates in a Pt-rich two-dimensional PtxRu1?x surface alloy provide active sites allowing nonactivated H2 dissociative adsorption. These two possible defects in a full pseudomorphic Pt monolayer deposited on Ru(0001) might be responsible for the relatively high initial sticking probabilities obtained experimentally for &#8764;1.0?1.2 ML of Pt evaporated over Ru(0001), only &#8764;30?65% smaller than on Ru(0001).","palabras_clave":"BIMETALLIC SURFACES|DISSOCIATIVE ADSORPTION|REACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4979,"idioma_id":4,"titulo":"Protective effects of retinoid X receptors on retina pigment epithelium cells","resumen":"Age-related macular degeneration (AMD) is among the main pathologies leading to blindness in adults and has currently no cure or effective treatment. Selective apoptosis of retina pigment epithelial (RPE) cells results in the progressive loss of photoreceptor neurons, with the consequent gradual vision loss. Oxidative stress plays an important role in this process. We have previously determined that activation of RXRs protects rat photoreceptor neurons from oxidative stress- induced apoptosis. In this study we investigated whether RXR ligands prevented apoptosis in an RPE cell line, D407 cells, exposed to hydrogen peroxide (H2O2). H2O2 induced apoptosis of D407 cells, promoting p65NF&#954;B nuclear translocation, increasing Bax mRNA expression, activating caspase-3 and altering cell morphology. We show, for the first time, that HX630, a RXR pan-agonist, protected D407 cells from H2O2-induced apoptosis, preventing p65NF&#954;B nuclear translocation, increasing Bclxl and PPAR&#947; mRNA levels and simultaneously decreasing Bax mRNA levels and caspase-3 activation. Pretreatment with a RXR antagonist blocked HX630 protection. LG100754, which binds RXRs but only activates heterodimers and is an antagonist of RXR homodimers, also had a protective effect. In addition, only agonists known to bind to RXR\/PPAR&#947; were protective. As a whole, our results suggest that RXR activation protects RPE cells from oxidative stress-induced apoptosis and this protection might involve signaling through a heterodimeric receptor, such as RXR\/PPAR&#947;. These data also imply that RXR agonists might provide potential pharmacological tools for treating retina degenerative diseases.","palabras_clave":"PIGMENT EPITHELIUM|RETINOID X RECEPTORS|OXIDATIVE STRESS|PREOXISOME PROLIFERATOR ACTIVATED RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4980,"idioma_id":5,"titulo":"Sistema reproductivo de Trichloris (Poaceae, Chloridoideae, Chlorideae)","resumen":"Trichloris crinita y T. pluriflora constituyen importantes forrajeras nativas con potencialidadesde ser domesticadas e introducidas al cultivo, pero es escasa la información básica sobre su biologíareproductiva. El objetivo de este trabajo fue determinar el sistema reproductivo de ambas especiesen poblaciones provenientes del centro-norte de Argentina. Se propuso: 1) establecer el sistemareproductivo a través de la relación fruto\/flor en polinizaciones controladas, 2) cuantificar el peso de losfrutos producidos, por población, bajo diferentes tratamientos y, 3) confirmar o descartar la presencia deapomixis a través de observaciones embriológicas. En T. crinita, la relación fruto\/flor promedio fue de92,4 ± 6,6%, mientras que en T. pluriflora, fue de 70,7 ± 9,9%. Estos resultados se corresponden conespecies autocompatibles, preferentemente autógamas. El desarrollo del saco embrionario indica origensexual de las semillas en ambas especies. Se hallaron diferencias significativas en el peso promedio decien cariopsis entre poblaciones y entre tratamientos, en ambas especies. Los frutos fueron más livianosen autopolinización. La información que se presenta es un aporte a las tareas de selección e introducciónal cultivo como también a la conservación y regeneración del germoplasma recolectado.","palabras_clave":"TRICHLORIS|ÉXITO REPRODUCTIVO|AUTOPOLINIZACIÓN|PESO DE SEMILLAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4981,"idioma_id":4,"titulo":"The use of fluctuating asymmetry as a measure of farming practice effects in rodents: A species-specific response","resumen":"The effects of agricultural intensification on vertebrate populations could vary depending on whether species are habitat specialists or habitat generalists. Organic farming practices are generally considered to be less intensive and more environmental friendly than conventional farming practices and, as a result, these two managements may impact on habitat specialists and habitat generalists in different ways. The effect of environmental and\/or genetic stress on  populations can be assessed using fluctuating asymmetry (FA) and body condition of animals. We predicted that populations of a specialist species, the Pampean grassland mouse (Akodon azarae) would have higher levels of FA and poorer body condition on conventional farms compared to populations of A. azarae on organic farms. In contrast, we predicted that populations of generalist species, the corn mouse (Calomys musculinus) and the small vesper mouse (C.alomys laucha) would not show differences in FA or body condition between conventional and organic farms. We examined the expression of FA in the hind foot and used the scaled mass index as a surrogate for body condition. As predicted, we found higher FA in the habitat specialist (A. azarae) on conventional farms compared to organic farms, and found no differences in FA among the two generalist species (C. musculinus and C. laucha). However, we found no differences in body condition for the three studied species between the two managements. Our results suggest that the effect of farming practices on small mammals varies between habitat specialists and habitat generalists. The results of this study provide important insights for the study of asymmetries, both from biological and methodological perspectives. Our results support the idea that the level of FA may be used as an index to assess the effects of farming practices on vertebrate populations.","palabras_clave":"Organic vs. conventional farm|Development instabilit|Specialist species|Generalist species|Small mammals|Stress indicator"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4982,"idioma_id":4,"titulo":"Electroretinography: A biopotential to assess the function \/  dysfunction of the retina.","resumen":"The Electroretinography (ERG) is a noninvasive technique that allows the assessment of functional integrity of the retina. The ERG recordings are biopotencials acquired in the corneal surface and as a response of retinal tissue against controlled light stimuli. In clinical ophthalmology ERG is not commonly used but nowadays, because of the high incidence of degenerative diseases of the retina (RD), their use should be increased. Like other biopotentials as electrocardiography (ECG), electroencephalogram (EEG) and electromyography (EMG), is a low amplitude signal, in this case a few hundred of microvolts (µV), which must be fitted and processed. The ERG signals are affected in morphology in the presence of pathologies that affects the integrity of the different retinal cell groups, for example due to some RD. In advanced cases of RD recordings can be abolished in the time domain; and yet in them it is believed that there is relevant clinical information making the ERG a great potential diagnostic tool.","palabras_clave":"Electrorretinografía|Degeneración retinal|Procesamiento de señales biomédicas|función retinal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4983,"idioma_id":4,"titulo":"Cranial Anatomy and Palaeoneurology of the Archosaur Riojasuchus tenuisceps from the Los Colorados Formation, La Rioja, Argentina","resumen":"Riojasuchus tenuisceps Bonaparte 1967 is currently known from four specimens, includingtwo complete skulls, collected in the late 1960s from the upper levels of the Los ColoradosFormation (Late Triassic), La Rioja, Argentina. Computed tomography (CT) scans of theskulls of the holotype and a referred specimen of Riojasuchus tenuisceps and the repreparationof the latter allows recognition of new features for a detailed analysis of its cranialanatomy and its comparison with a wide variety of other archosauriform taxa. The diagnosisof Riojasuchus tenuisceps is emended and two autapomorphies are identified on the skull:(1) a deep antorbital fossa with its anterior and ventral edges almost coinciding with thesame edges of the maxilla itself and (2) a suborbital fenestra equal in size to the palatinepterygoidfenestra. Also, the first digital 3D reconstruction of the encephalon of Riojasuchustenuisceps was carried out to study its neuroanatomy, showing a shape and cranial nervedisposition consistent to that of other pseudosuchians.","palabras_clave":"Archosuria|Ornithosuchia|anatomy|paleoneuology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4984,"idioma_id":4,"titulo":"In vivo xenoestrogenic actions of cadmium and arsenic in anterior pituitary and uterus","resumen":"Cadmium (Cd) and arsenic (iAs) are toxic metals ubiquitously present in the environment. Both pollutants exert nonmonotonic dose responses, being mostly cytotoxic at high concentrations but mimicking estrogen (E2) effects at low doses. Xenoestrogenic activity of Cd and iAs has been demonstrated in different hormone-dependent tumor cell lines; however, their actions in vivo remain largely unknown. Here, we investigated whether in vivo administration of low doses of Cd and iAs through drinking water would display xenoestrogenic effects in the anterior pituitary gland and uterus of ovariectomized rats. Cd (1ppm) and iAs (0.1ppm) exposure increased the wet weight of anterior pituitary gland and uterus and induced proestrus- and estrus-like vaginal smears. Both metals stimulate cell proliferation of these tissues as they increased the expression of proliferation markers. More importantly, they augmented soluble guanylyl cyclase &#945;1 subunit expression, which has been linked to hormone-dependent tumor progression. Also, Cd and iAs modified protein levels of full-length estrogen receptor &#945; and its truncated variants in an E2-like manner. Anterior pituitary hormone secretion was differentially affected by both metals. Luteinizing hormone synthesis and release were strongly diminished after Cd exposure and only mildly reduced by iAs. Both metals were able to increase prolactin synthesis, although only iAs augmented serum prolactin levels. This study shows for the first time that Cd and iAs exert strong xenoestrogenic effects on anterior pituitary gland at low doses. The differences between Cd and iAs E2-like behavior indicate that other Cd- and iAs-specific mechanisms could be involved. Altogether, these results contribute to the knowledge of reproductive disorders associated with Cd and iAs environmental contamination.","palabras_clave":"CADMIUM|ARSENIC|XENOESTROGENIC-ACTION|UTERUS|ANTERIOR-PITUITARY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4985,"idioma_id":5,"titulo":"Las normativas del CONICET y la UNLP sobre la mención de la afiliación institucional en las publicaciones científicas. Problemas recurrentes y nuevas tensiones en el sistema científico y tecnológico argentino","resumen":"Recientes normativas sobre el contenido y forma de mencionarla afiliación institucional de los investigadores de dos de las principales instituciones públicas de investigación de Argentina (la Universidad Nacional de La Plata y el Consejo Nacional de Investigaciones Científicas y Técnicas) obligan a reflexionar sobre problemas recurrentes y nuevas tensiones en torno a las relaciones institucionales del sistema científico­tecnológico argentino. Los objetivos de esta contribución son: (1) analizar las normativas de ambas instituciones&#894; (2) señalar sus principales virtudes y falencias&#894; y (3) alertar sobre los peligros de promulgar reglamentaciones no consensuadas. Las diferentes y hasta contradictorias normativas referidas a cómo los científicos deben registrar su afiliación institucional en las publicaciones están basadas en miradas aisladas, que colocan a numerosos investigadores con doble dependencia institucional en una encrucijadacuando deben cumplirlas. Los ejemplos analizados exponen la falta de coordinación interinstitucional y la necesidad de tener una mirada que contémplelas relaciones de cooperación nacional, regional e internacional en un escenario científico y tecnológico cada vez más globalizado. No parece razonable promulgar normativas que pongan a las instituciones estatales en una suerte de competencia por apropiarse de la producción científica de los investigadores, canibalizándose unas a otras. A contramano de la historia y del espíritu de colaboración que ha primado dentro del sistema argentino de ciencia y tecnología, estas visiones sesgadas ponen en serio riesgo el lugar privilegiado que ha logrado la Argentina entre los principales países productores de ciencia de América Latina, así como el reconocimiento internacional de sus instituciones científico­tecnológicas.","palabras_clave":"Política científico­-tecnológica|Cienciometría|Investigación científica|Afiliación institucional|Publicaciones científicas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4986,"idioma_id":4,"titulo":"Taxonomy of the archosaur Ornithosuchus: reassessing Ornithosuchus woodwardi Newton 1894 and Dasygnathoides longidens (Huxley 1877)","resumen":"The pseudosuchian archosaur Ornithosuchus, from the Lossiemouth Sandstone Formation (Late Triassic), Scotland, was the first ornithosuchid to be discovered and the only one recorded in the northern hemisphere. The fossil record of ?Ornithosuchus longidens? is mainly based on natural moulds and, in a few cases, three-dimensional bony elements, complicating the interpretation of its anatomy. The taxonomy of this species has changed several times since the late 1800s and here we revisit its current status. The synonymy of ?Dasygnathoides longidens? and Ornithosuchus woodwardi proposed by Walker (1964) is rejected based on new interpretations of the hypodigm of ?Dasygnathoides longidens?. This last species is here considered as a nomen dubium because it lacks diagnostic features and cannot be identified beyond Pseudosuchia. As a result, Ornithosuchus woodwardi is resurrected as a valid species and its diagnosis is emended. The body size range of Ornithosuchus woodwardi is reduced to about a half, because EM 1, previously considered the largest specimen of the genus, can no longer be referred to it. ?Dasygnathoides longidens? cannot be assigned to any of the known archosauriforms from the Lossiemouth Sandstone Formation, but it still represents the largest predator currently known for its fauna.","palabras_clave":"TAXONOMY|PSEUDOSUCHIA|SCOTLAND|TRIASSIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4987,"idioma_id":4,"titulo":"Azimuthal asymmetry in the risetime of the surface detector signals of the Pierre Auger Observatory","resumen":"The azimuthal asymmetry in the risetime of signals in Auger surface detector stations is a source of information on shower development. The azimuthal asymmetry is due to a combination of the longitudinal evolution of the shower and geometrical effects related to the angles of incidence of the particles into the detectors. The magnitude of the effect depends upon the zenith angle and state of development of the shower and thus provides a novel observable, (sec &#952;)_max , sensitive to the mass composition of cosmic rays above 3 × 10^18 eV. By comparing measurements with predictions from shower simulations, we find for both of our adopted models of hadronic physics (QGSJETII-04 and EPOS-LHC) an indication that the mean cosmic-ray mass increases slowly with energy, as has been inferred from other studies. However, the mass estimates are dependent on the shower model and on the range of distance from the shower core selected. Thus the method has uncovered further deficiencies in our understanding of shower modeling that must be resolved before the mass composition can be inferred from (sec&#952;)_max.","palabras_clave":"PIERRE AUGER OBSERVATORY|COSMIC RAYS|SURFACE DETECTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4988,"idioma_id":5,"titulo":"República Checa: una estadía en el corazón de Europa","resumen":"Una estancia corta en República Checa en el marco de un proyecto bilateral con la Republica Argentina, nos permitió conocer acerca de la producción, investigación y cultura de ese país.","palabras_clave":"Brassica|Centro de Investigacion|Republica Checa|Mejoramiento"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4989,"idioma_id":5,"titulo":"Ambiente y Plancton en la Zona Común de Pesca Argentino-Uruguaya en un escenario de cambio climático (marzo, 2014)","resumen":"En este trabajo se presentan resultados de una campaña ambiental multidisciplinaria realizada en la Zona Común de Pesca (ZCP) en el BIP 'Cap. Oca Balda' (26 de Marzo al 1 de Abril de 2014). Los muestreos se distribuyeron en dos secciones en la plataforma continental desde el sector costero (COS) hasta trasponer el talud continental (TAL), a las latitudes de Mar del Plata (Argentina) y La Paloma (Uruguay), denominándose COSTAL I y COSTAL II, respectivamente. En cada una se ubicaron seis estaciones,  en las que se siguió una misma secuencia de actividades comenzando con la adquisición de los perfiles de temperatura, salinidad, fluorescencia y penetración de luz. Luego, se tomaron muestras de agua con botellas 'Niskin', las cuales se utilizaron para analizar nutrientes, oxígeno disuelto, alcalinidad total - carbono inorgánico disuelto, absorción del material particulado y del orgánico disuelto coloreado (CDOM), concentración de clorofila a total (ClaT) y la debida a la fracción del fitoplancton menor a 5 µm (Cla<5), diversidad de pigmentos, abundancia del bacterioplancton, búsqueda y recuento de microorganismos enteropatógenos, bacterias degradadoras de hidrocarburos (BDH) y de todas las fracciones del fitoplancton. Finalmente, se realizaron muestreos con diferentes redes de plancton: mini-Bongo (67 y 200 µm), Bongo (300 µm) y Nackthai (400 µm).En las dos secciones las temperaturas fueron menores a la media de verano, destacándose la COSTAL I por presentar una situación de comienzos del otoño, señalada además por la mayor disponibilidad de nitrato. En cuanto a la distribución de masas de agua, se destacaron en la COSTAL II las Aguas del Río de la Plata extendidas hacia el este hasta unos 200 km, posiblemente explicado por el régimen de vientos presentes. La zona eufótica se profundizó desde la costa hacia el talud en la COSTAL II, mientras que en la otra sección se mantuvo alrededor de los 40 m. Los valores del coeficiente de absorción específico del fitoplancton estuvieron directamente relacionados con la dominancia de los componentes del ultrafitoplancton. La absorción del CDOM fue significativamente alta en la COSTAL II y mostró una fuerte relación inversa con la salinidad. En la COSTAL II, la concentración de ClaT fue mayor en la zona costera y disminuyó progresivamente hacia el talud, mientras que en la COSTAL I valores altos de ClaT persistieron a lo largo de la plataforma en superficie. En ambas secciones la concentración Cla<5 fue alta. En cuanto a la diversidad pigmentaria, la COSTAL II se distinguió por las altas concentraciones de zeaxantina en las estaciones más costeras y el registro de DV-Cl a que señala la presencia de Prochlorococcus en la estación 6U en aguas tropicales. En la COSTAL I es notoria la presencia de 19´hexanoiloxifucoxantina y Cl c3 indicando la importancia de las haptofitas en el área, y MV-Cl c3, sólo en la estación 4 coincidiendo con la presencia de Emiliania huxleyi. Si bien los valores de biomasa de fitoplancton expresados en valores de carbono fueron similares en ambas secciones con un rango de ~10?68 mg C m-3, la distribución espacial presentó particularidades en cada una de ellas: en la COSTAL II, las mayores concentraciones se limitaron a las estaciones costeras (1U-3U), mientras que en la COSTAL I se ubicaron en las estaciones 2 y 4 en capas profundas y en superficie en la estación 6. Considerando las tres fracciones analizadas (micro, nano y ultrafitoplancton) en toda el área prevaleció la fracción más pequeña, aunque mostrando diferencias cualitativas. El bacterioplancton alcanzó concentraciones similares en ambas secciones con valores máximos de ~45 mg C m-3 con una distribución diferencial, ubicándose en profundidad en las estaciones más costeras en la COSTAL II y en aguas externas en la COSTAL I (estación 5). Se registraron BDH en el sector costero de las dos secciones  y se confirmó la presencia de Vibrio parahaemolyticus, pero sólo en las estaciones más costeras de la COSTAL II. El ultra y el microprotozooplancton  alcanzaron sus máximas biomasas (~8 mg C m-3) en aguas superficiales de las estaciones de plataforma externa en la COSTAL I y las del sector costero en la COSTAL II respectivamente. De los componentes del zooplancton se analizaron particularmente todos los estadios de desarrollo de los copépodos, siendo los mejor representados las nauplii y los copepoditos que, en conjunto constituyeron el 78,71% de la comunidad, particularmente los pertenecientes a los órdenes Calanoida (< 1 mm) y Cyclopoida (< 1 mm). La máximas abundancias (~47000 ind. m-3) se registraron en la estación 2U. Las nauplii sólo alcanzaron porcentajes elevados (50%) en la estación 2U de la COSTAL II, representando hasta el 70% en las estaciones 1 - 5 y 6 de la COSTAL I. En cuanto a los copepoditos, tuvieron una distribución diferencial en ambas secciones, alcanzando una elevada representación en la COSTAL II, tanto los Cyclopoida (entre 20-65%) como los Calanoida, mientras que en la COSTAL I se distinguió este último grupo con porcentajes entre 80 y 50% en las estaciones 2 a 4. En cuanto a la distribución y abundancia de huevos de anchoíta, las mayores densidades se observaron en la estación 3 de la COSTAL I (600 huevos m-2), mientras que fue muy pobre en la COSTAL II. Se detectó la presencia de larvas de anchoíta en 8 de las 12 estaciones estudiadas destacándose las estaciones 5U (222 ind. m-2) de la COSTAL II y la 3 (430 larvas m-2) de la COSTAL I. Se estudió la condición nutricional de las larvas, evidenciándose por la relación ADN\/ARN y la performance de crecimiento, que se encontraban en una buena condición nutricional. En general, la estación 6U mostró características particulares en las variables consideradas debido a la presencia de aguas tropicales, distinguiéndose como un sistema completamente diferente al resto de las estaciones.Todos estos resultados nos permiten señalar que, a pesar de las diferencias en las condiciones ambientales, en ambas secciones se desarrollaron tramas tróficas microbianas en las cuales los productores primarios son principalmente células pequeñas (<5 µm). Este tipo de tramas planctónicas tienen la más alta eficiencia trófica debido al fuerte acoplamiento entre los niveles tróficos del plancton, por lo que canalizan la mayor parte del carbono a la producción de peces pelágicos, y por ende hay una limitada exportación de carbono biogénico desde el sistema pelágico. A pesar de la uniformidad en el tipo de trama trófica, se evidenció una distribución diferencial cuali-cuantitativa de los diversos niveles tróficos analizados en relación con el ambiente físico-químico y lumínico.Considerando el escenario de cambio climático en que se encuentra hoy nuestro planeta, con algunos efectos ya registrados en el océano mundial, es muy importante contar con observaciones continuas del ambiente marino. Esta información debe provenir no sólo de sensores remotos y boyas, sino también de mediciones in situ de variables ambientales y principalmente de la biología del océano, comprendiendo la taxonomía y fisiología del plancton, la estructura de las comunidades, dinámica de las tramas tróficas y procesos mesopelágicos.El presente estudio multidisciplinario en la ZCP constituye el primero de estas características en el área y aporta un conocimiento fundamental para la comprensión del circuito de la materia en este sistema tan complejo. Considerando que esta información es limitada a una época del año, sería muy importante poder repetir este tipo de estudios con periodicidad estacional.","palabras_clave":"OCEANOGRAFÍA|FITOPLANCTON|ZOOPLANCTON|ICTIOPLANCTON|CONDICIÓN NUTRICIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4990,"idioma_id":4,"titulo":"MicroRNA discovery in the human parasite Echinococcus multilocularis from genome-wide data (IF 2.284)","resumen":"The cestode parasite Echinococcus multilocularis is the aetiological agent of alveolar echinococcosis, responsible for considerable human morbidity and mortality. This disease is a worldwide zoonosis of major public health concern, considered a neglected disease by the World Health Organisation. The motivation for this work is the discovery of new miRNAs based on non target genomics data only, in order to make sense the unexplored genomic data currently available. We have extracted the most commonly used structural features from the parasite genome folded sequences: and used them to train a novel deep architecture of self-organizing maps. We discovered 886 candidates to pre-miRNAs and 23 were experimentally validated. The methodology used can be easily adapted and applied on any draft genomes, which are actually the most interesting ones since most of non model organism have this kind of status and have a real biological and sanitary relevance.","palabras_clave":"GENOME-WIDE DATA|SELF ORGANIZING MAPS|MIRNA|HIGH CLASS IMBALANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4991,"idioma_id":4,"titulo":"Diversity control for improving the analysis of consensus clustering (IF 4.038)","resumen":"Consensus clustering has emerged as a powerful technique for obtaining betterclustering results, where a set of data partitions (ensemble) are generated,which are then combined to obtain a consolidated solution (consensus partition)that outperforms all of the members of the input set. The diversityof ensemble partitions has been found to be a key aspect for obtaining goodresults, but the conclusions of previous studies are contradictory. Therefore,ensemble diversity analysis is currently an important issue because thereare no methods for smoothly changing the diversity of an ensemble, whichmakes it very dicult to study the impact of ensemble diversity on consensusresults. Indeed, ensembles with similar diversity can have very dierent properties,thereby producing a consensus function with unpredictable behavior.In this study, we propose a novel method for increasing and decreasing thediversity of data partitions in a smooth manner by adjusting a single parameter,thereby achieving ne-grained control of ensemble diversity. The resultsobtained using well-known data sets indicate that the proposed method iseective for controlling the dissimilarity among ensemble members to obtaina consensus function with smooth behavior. This method is importantfor facilitating the analysis of the impact of ensemble diversity in consensusclustering.","palabras_clave":"cluster ensembles|consensus clustering|diversity analysis|diversity control"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4992,"idioma_id":4,"titulo":"Biological substrates: green alternatives in trace elemental preconcentration and speciation analysis","resumen":"Biological substrates have been introduced in Analytical Chemistry to encourage the development of environment-friendly methodologies. This kind of substrates offers advantages such as low cost, very simple production and biodegradability. Moreover, they are considered highly efficient materials for extraction and separation of elemental species.The current article reviews the applications of biological substrates in elemental preconcentration and speciation analysis, with emphasis in the latest analytical methodologies developed in this field. Batch and on-line microextraction techniques, based on biological substrates are presented and discussed. The applications of immobilized biological substrates are also commented in this work. Special attention is given to the novel immobilization of these substrates on nanomaterials and nanoparticles to develop solid phase extraction techniques. A comparison of methods using biological substrates in terms of analytical performance is provided. Finally, future trends, developments and challenges related to the use of biological substrates for trace metal determination are discussed.","palabras_clave":"BIOLOGICAL SUBSTRATES|BIOSORPTION|PRECONCENTRATION|SPECIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4993,"idioma_id":4,"titulo":"Hypercarnivorous apex predator could provide ecosystem services by dispersing seeds","resumen":"Large ?hypercarnivorous? felids are recognized for their role as apex predators and hence as key elements in food webs and ecosystem functioning through competition and depredation. Here we show that cougars (Puma concolor), one of the largest and the most widely ranging apex felid predators with an strictly carnivorous diet, could be also effective secondary long distance seed dispersers, potentially establishing direct and non-herbivore mediated interactions with plant species at the bottom of the food web. Cougars accidently ingest and disseminate large amounts of seeds (31,678 seeds in 123 scats) of plant species initially consumed by their main prey, the Eared Dove Zenaida auriculata. The germination potential of seeds for three of the weedy\/invasive plant species more abundantly found in cougar scats (19,570 seeds) was not significantly different from that observed in seeds obtained from dove gizzards, indicating that seed passage through cougar guts did not affect seed germination. Considering the estimated cougar density in our study area, dispersal of seeds by cougars could allow a mean, annual seed spread of ~5,000 seeds per km2. Our results demonstrate that strictly carnivorous, felid predators could have broad and overlooked ecological functions related to ecosystem structuring and functioning.","palabras_clave":"Secondary seed dispersal|Apex predators|Large felids|Cougar"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4994,"idioma_id":5,"titulo":"Inicios de la Psicología en Córdoba, Argentina: Psicología Experimental, Clínica y Social (1895-1918)","resumen":"Este trabajo explora las primeras discusiones sobre psicología (1895-1918) en la ciudad de Córdoba,Argentina. Para ello se reconstruyeron series documentales a partir de tesis de medicina, publicacionesen revistas médicas y científicas de la Universidad Nacional de Córdoba, así como manuales detoxicomanía y medicina legal. El discurso psicológico tuvo su evolución en el marco del de las cienciashumanas, tras un período de gran influencia del naturalismo positivista, por lo que es factor relevanteen la clínica psiquiátrica, la criminología y la sociología. El trabajo evidencia la presencia de la psicologíafrancesa, las discusiones en torno a su cientificidad y la influencia de la filosofía y psicologías ligadas alvitalismo español. Las fuentes relevadas se abordaron desde una perspectiva histórica crítica, ligada a lahistoria intelectual y los estudios de recepción","palabras_clave":"HISTORIA|PSICOLOGIA|CORDOBA|CIENCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4995,"idioma_id":5,"titulo":"Evaluación académica y relevancia socioproductiva: los Proyectos de Desarrollo Tecnológico y Social (PDTS) como política científica","resumen":"En este artículopresentamos los primeros resultados de la investigación que estamos llevando acabo en torno a la recepción y el desarrollo de una iniciativade política científica argentina, los Proyectos de Desarrollo Tecnológico ySocial (PDTS) tendiente a orientar la investigación científica hacia formas deconocimiento que contribuyan a la solución de problemas sociales, a lasdemandas socio-productivas, a prioridades definidas por los organismos deciencia y tecnología o al aprovechamiento de una oportunidad estratégica demercado. La iniciativa comenzó a gestarse en 2012 y su implementación a partirde 2013. Este instrumento de política científica coordinado y articulado por elMinisterio de Ciencia, Tecnología e Innovación Productiva de la NaciónArgentina pero propuesto y puesto en marcha por varios organismos, se encuadraen otras medidas y pautas que buscaban orientar el viejo y afianzado complejode ciencia y tecnología hacia nuevas funciones y misiones o a ampliarlas (OTEIZA,1992). Nos proponemosenmarcar dicho análisis en algunos de los nuevos conceptos estelares(BRASLAVSKY, 1999) que impactan en la toma de decisiones y las orientaciones delas acciones de dichos organismos aunque los orígenes de las mismas no seansiempre locales, tales como los constituyen las categorías de movilización delconocimiento y de innovación social, la innovación para la inclusión social yla innovación sustentable.","palabras_clave":"POLITICA CIENTIFICA|PROYECTOS DE DESARROLLO TECNOLOGICO Y SOCIAL|RELEVANCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4996,"idioma_id":4,"titulo":"Follicular Cysts: a Single Sign and Different Diseases. A View from  Comparative Medicine","resumen":"Ovarian cystic follicles are the sign of important causes of reproductive failure in numerous species. In this review, some morphological, endocrinological and clinical aspects of cystic follicles in women, cows, mares, sows and bitches are discussed. Follicular cysts are the consequence of the failure of a mature follicle to ovulate at the appointed time of ovulation in the estrous cycle. Although the etiology of follicular cysts remains unknown, this review examines the evidence about the role of endocrine signaling systems in the specific disease or syndrome in each of the species mentioned above. This review also describes, the changes in the pathways of endocrine mechanisms that would trigger disturbances in the intraovarian component underlying the aberrant persistence of follicular cysts. The knowledge of the morphological and endocrinological nature of cystic follicles in different species can provide relevant information to better understand specific diseases when it is integrally analyzed from the comparative medicine viewpoint.","palabras_clave":"follicular cyst|polycystic ovarian syndrome|cystic ovarian disease|cow|sow|mare|bitch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4997,"idioma_id":5,"titulo":"Participación del sistema IGF en la patogenia de la enfermedad quística  ovárica bovina","resumen":"Laenfermedad quística ovárica (COD) es una de las principales causas de fallareproductiva y de infertilidad, y constituye uno de los de los trastornosreproductivos más frecuentes en vacas lecheras de alta producción. Eldesarrollo de esta enfermedad está asociado a un desequilibrio en el ejehipotalámico-hipofisario-gonadal en el cual factores endocrinos, entre ellos elsistema de factores de crecimiento análogos a insulina (IGF), participan en eldesarrollo folicular, diferenciación celular y la secreción de hormonasováricas. Considerando al sistema IGF como un importante regulador delcrecimiento y funcionalidad folicular, evaluamos la participación decomponentes del sistema IGF en el desarrollo de la COD. Las alteraciones endiferentes componentes del sistema IGF que podrían afectar al normalfuncionamiento del ovario participando en el desarrollo de la COD en bovinos.","palabras_clave":"SISTEMA DE FACTORES DE CRECIMIENTO ANÁLOGOS A INSULINA|ENFERMEDAD QUÍSTICA OVÁRICA|BOVINOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4998,"idioma_id":4,"titulo":"Colonization and Succession of Zooplankton After a Drought: Influence of Hydrology and Free-Floating Plant Dynamics in a Floodplain Lake","resumen":"We analyzedzooplankton colonization and succession following a temporary drought in avegetated lake from a floodplain wetland of the Lower Paraná River (Argentina).We took samples of zooplankton and phytoplankton at four habitats of the lakecharacterized by the presence-absence of emergent or free-floating macrophytesalong a 18-month period after the drought. Microzooplankton dominated along thestudy and macrozooplankton showed seasonal increments in cold seasons. Temporalpatterns of broad taxonomic zooplankton groups were associated tohydrology-driven factors, like changes in water depth, conductivity and ediblephytoplankton and seasonal changes and not related with free floating plants.Nevertheless, spatial and temporal patterns of species composition were closelylinked to the dynamics of free-floating plants. A clear shift from pelagic tolittoral zooplankton taxa was registered when free-floating plants colonized. Thisstudy expands the knowledge on the composition and dynamics of zooplankton fromfloodplain lakes after the disturbance caused by a severe drought. Our resultshighlight the influence of water level fluctuations as drivers of zooplankton abundanceand the joint effect of seasonal changes and free-floating plant dynamics onzooplankton species composition and richness.","palabras_clave":"COMMUNITY COMPOSITION|DISTURBANCE|HABITAT HETEROGENEITY|LITTORAL AND PELAGIC SPECIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":4999,"idioma_id":4,"titulo":"Circular structures of Bajada del Diablo (Argentina): Geophysical Signatures","resumen":"Bajada del Diablo is located in the Northern Patagonian Massif, Chubut, Argentina. The study area includes several circular structures found in Miocene olivine basalts of the Quiñelaf Eruptive Complex and in the Late Pliocene\/Early Pleistocene Pampa Sastre conglomerates. An impact origin has been proposed for these circular structures. With the aim of further investigate the proposed impact origin, topographic, gravimetric, magnetic, resistivity, paleomagnetic and electromagnetic surveys in two circular structures (?8? and ?G?) located in Pampa Sastre conglomerates and in basalts of the Quiñelaf Eruptive Complex were carried out. The new geophysical results support the hypothesis of an impact origin. However, the confirmation of such an origin through the findings of shock metamorphism evidences and\/or the recovery of meteorites remains elusive.","palabras_clave":"CIRCULAR STRUCTURES|IMPACT ORIGIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5000,"idioma_id":4,"titulo":"Response of fish assemblage structure to changing flood and flow pulses in a large subtropical river","resumen":"FishassemblagesrecordedfromAugust2010toJuly2013intwoloticandlenticenvironmentsoftheMiddle ´ periodofstudy,eachwithadifferentmagnitude,durationandtiming.Insteadfourflowpulseswererecordedduringthis period.Thevariedhydrologicalconditionshadaninfluenceontheichthyofauna.Thefishspeciesrichnessinbothlentic and lotic habitatwas negatively correlatedwith themaximumwater level and fishassemblageswere spatially and temporallystructuredaccordingtotheirdifferentreproductivestrategies.Long-andshort-distancemigratoryspecies weredominantafterthefloodpulseofthehighestintensityandlongestduration,whereassedentaryfishandinternal fertilisationprevailedduringflowpulsesandlowerfloodpulses.Fishassemblagesimilaritywasgenerallylowbetween habitatsduringfloodandflowpulses,butincreasedafterthoseeventsdependingontheirmagnitude,durationandtiming. Apparently, flow pulses would also function like floods, as mechanisms of spatio-temporal structuring of fish Parana Riverfloodplainwerestudiedinthelightoffloodandflowpulsevariations.Threefloodpulsesoccurredduringthe assemblages.","palabras_clave":"FLOODPLAIN|ICHTHYOFAUNA|PARANÁ RIVER|TEMPORAL?SPATIAL EFFECTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5001,"idioma_id":4,"titulo":"Local scale variation in  the reproductive pattern of the southern geoduck, Panopea abbreviata (Bivalvia:  Hiatellidae), in Patagonia","resumen":"During 2007 was carried out studies to explore local scale spatial variability in the reproductive cycle of Panopea abbreviata in three sites of the northern Patagonian gulfs (El Sótano and Puerto Lobos, in San Matías Gulf, and Punta Conos in San José Gulf). Previous reports have revealed that P. abbreviata exhibits a reproductive pattern characterized, in both sexes, by continuous proliferation of gametes and spawning throughout the year. However, the use of quantitative indicators for females has shown a slight seasonality to this pattern. Local spatial variability in the reproductive cycle of P. abbreviata was observed at three sites and related to variation in the annual temperature regime at each locality. The females of El Sótano and Puerto Lobos showed similar seasonality in the reproductive pattern: proliferation and maturation phases prevailed over spawning stages when temperature decreased during autumn (March-May); this pattern was reversed when temperatures began to increase during spring (September-November). At Punta Conos this pattern is more irregular. However, at all localities, mature individuals of both sexes were present throughout the year, without a resting period during the reproductive cycle. The reproductive pattern is similar in the three sites with many small pulses in reproductive activity that are difficult to detect and characterized by year-round spawning. The same pattern was found in other localities of P. abbreviata and is unique in the genus Panopea.","palabras_clave":"GEODUCK|PANOPEA ABBREVIATA|GAMETOGENESIS|REPRODUCTIVE CYCLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5002,"idioma_id":4,"titulo":"Antithrombotic and Antioxidant Activity of Amaranth Hydrolysate Obtained by Activation of an Endogenous Protease","resumen":"Abstract Ingestion of diets with antithrombotic and antioxidantcomponents offer a convenient and effective way to preventand reduce the incidence of cardiovascular diseases. Theaimof the present work was to obtain an amaranth hydrolysateby the activation of an endogenous aspartic protease, to establishadequate experimental conditions, and to evaluate its antithromboticand antioxidant activity in order to assess its potentialapplication as an ingredient in functional foods. Theresults obtained not only confirmed the presence of anendogenous protease in the amaranth isolate, but alsoallowed us to select an adequate incubation conditions(pH 2, 40 °C, 16 h). The hydrolysate obtained (degreeof hydrolysis 5.3 ± 0.4 %) showed potential antithromboticactivity (IC50 = 5.9 ± 0.1 mg soluble protein\/mL) andhad more antioxidant activity than the isolate, indicatingthat the activation of the protease released bioactive peptidesfrom amaranth proteins. Decreasing the pH is a simpleand cheap process and is another way to obtain potentialfunctional ingredients with bioactive compounds.","palabras_clave":"Amaranth proteins|Endogenous aspartic protease|Bioactive peptides|Antithrombotic and antioxidant activity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5003,"idioma_id":4,"titulo":"Computational study of basis set and electron correlation effects on anapole magnetizabilities of chiral molecules","resumen":"In the presence of a static, nonhomogeneous magnetic field, represented by the axial vector B at the origin of the coordinate system and by the polar vector C=&#8711;Ã?B, assumed to be spatially uniform, the chiral molecules investigated in this paper carry an orbital electronic anapole, described by the polar vector A. The electronic interaction energy of these molecules in nonordered media is a cross term, coupling B and C via &#257;, one third of the trace of the anapole magnetizability a&#945;&#946; tensor, that is, WBC=-&#257;B·C. Both A and WBC have opposite sign in the two enantiomeric forms, a fact quite remarkable from the conceptual point of view. The magnitude of &#257; predicted in the present computational investigation for five chiral molecules is very small and significantly biased by electron correlation contributions, estimated at the density functional level via three different functionals.","palabras_clave":"ANAPOLOS|GRADIENTE DE CAMPO MAGNETICO|CORRELACION|QUIRALIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5005,"idioma_id":4,"titulo":"Habitat use by armadillos in agroecosystems of central Argentina: does plot identity matter?","resumen":"In agroecosystems, the use of cultivated plots by mammals depends on habitat structure as well as on the degree of species specialization. In the Pampas of Argentina, during the last 2?3 decades, there has been a continuing expansion of cropland. The aim of this study was to analyze how the identity and characteristics of agricultural plots may have affected the activity of 2 species of armadillos (Chaetophractus villosus and Dasypus hybridus) in a rural landscape. We carried out 4 sampling sessions between December 2011 and June 2013, surveying 175 plots during spring?summer and 194 during fall at 25 different sites in the Pampas of Buenos Aires province, Argentina. In each plot, we surveyed for signs (burrows and holes) along a 600×6 m transect in order to assess armadillo activity and measured 4 habitat structural variables. As expected, there were structural differences between types of agricultural plots. Across all plots, a total of 6,654 armadillo signs were found (C. villosus = 5,009, D. hybridus = 1,645). Generalized linear mixed models revealed that during both seasons, the type of plot explained much of the variation in armadillo activity, except during spring?summer for D. hybridus. C. villosus had higher use of plots with a longer history of non-tillage (e.g., soybean), while there was more evidence of D. hybridus activity in plots with less human intervention (e.g., grasslands), especially during fall. Overall, D. hybridus and C. villosus were more sensitive to the particular structural features of plots rather than to the kind of land use practiced (crop field or rangeland). These results have important implications for future management decisions in the Pampas region because during the last decades, farming of soybeans has expanded at the expense of grasslands, and this change appears to have differentially affected these 2 species of armadillos.","palabras_clave":"Chaetophractus|crop fields|Dasypus|perturbation|rangeland|soybean"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5006,"idioma_id":4,"titulo":"Inhibition of Hyaluronic Acid Synthesis Suppresses Angiogenesis in Developing Endometriotic Lesions","resumen":"AbstractBACKGROUND:The development and long-term survival of endometriotic lesions is crucially dependent on an adequate vascularization. Hyaluronic acid (HA) through its receptor CD44 has been described to be involved in the process of angiogenesis.OBJECTIVE:To study the effect of HA synthesis inhibition using non-toxic doses of 4-methylumbelliferone (4-MU) on endometriosis-related angiogenesis.MATERIALS AND METHODS:The cytotoxicity of different in vitro doses of 4-MU on endothelial cells was firstly tested by means of a lactate dehydrogenase assay. The anti-angiogenic action of non-cytotoxic doses of 4-MU was then assessed by a rat aortic ring assay. In addition, endometriotic lesions were induced in dorsal skinfold chambers of female BALB\/c mice, which were daily treated with an intraperitoneal injection of 0.9% NaCl (vehicle group; n = 6), 20mg\/kg 4-MU (n = 8) or 80mg\/kg 4-MU (n = 7) throughout an observation period of 14 days. The effect of 4-MU on their vascularization, survival and growth were studied by intravital fluorescence microscopy, histology and immunohistochemistry.MAIN RESULTS:Non-cytotoxic doses of 4-MU effectively inhibited vascular sprout formation in the rat aortic ring assay. Endometriotic lesions in dorsal skinfold chambers of 4-MU-treated mice dose-dependently exhibited a significantly smaller vascularized area and lower functional microvessel density when compared to vehicle-treated controls. Histological analyses revealed a downregulation of HA expression in 4-MU-treated lesions. This was associated with a reduced density of CD31-positive microvessels within the lesions. In contrast, numbers of PCNA-positive proliferating and cleaved caspase-3-positive apoptotic cells did not differ between 4-MU-treated and control lesions.CONCLUSIONS:The present study demonstrates for the first time that targeting the synthesis of HA suppresses angiogenesis in developing endometriotic lesions. Further studies have to clarify now whether in the future this anti-angiogenic effect can be used beneficially for the treatment of endometriosis.","palabras_clave":"ANGIOGENESIS|ENDOMETRIOSIS|HIALURONIC ACID|MOUSE MODEL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5007,"idioma_id":4,"titulo":"The clam Neilonella sulculata in San Jorge Gulf (Argentina, Southwest Atlantic): spatial distribution pattern and inverse relationship between size and density","resumen":"Neilonellasulculata is adominant bivalve on muddy bottoms of the continental shelf and gulfs offArgentina (Southwest Atlantic). Two benthic surveys performed in 2007 and 2009 in San Jorge Gulf, adepositional area characterized by silt\/clay sediments, gave us the opportunityto test whether the population of this clam displays a significant spatial structure and to analyse if there isan inverse relationship between size and density. Density, biomass and size?frequency distribution displayed significant spatial structure, being positively autocorrelatedat the smallest spatial scales (c. 1.7?14\/17 km). Biomass also showed spatial contagion atscales of 25?33 km. A substantial increase in density andbiomass occurred between 2007 and 2009. Empty valves in the sediment showedthat the maximum size attained by the species may exhibit considerablevariation at relatively short temporal scales. Regardless of temporal changesin density and biomass, the spatial structure of these variables remainedstable. In densely populated areas, shell size decreased with increasing density,suggesting a density-dependent control of growth. A clear north?south increase in density and biomass was detected,which might be related to a gradient in food availability caused by athermohaline frontal system associated with wind-related upwelling.","palabras_clave":"AUTOCORRELATION|BENTHIC INFAUNA|BIOMASS|MANTEL TEST|MORAN'S I|SIZE STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5008,"idioma_id":4,"titulo":"FLEXIBLE POLYURETHANE FOAMS MODIFIED WITH BIO-BASED POLYOLS: SYNTHESIS AND PHYSICAL\/ CHEMICAL CHARACTERIZATION","resumen":"A series of flexible polyurethane foams with different polyol compositions were synthesized through the replacement of a portion of the petroleum-based polyether polyol with biobased polyols, namely, glycerol (GLY) and hydroxylated methyl esters (HMETO). HMETO was synthesized by the alkaline transesterification of tung oil (TO; obtaining GLY as a byproduct) and the subsequent hydroxylation of the obtained methyl esters with performic acid generated in situ. FTIR spectroscopy, 1H-NMR, and different analytical procedures indicated that the hydroxyl content increased significantly and the molecular weight decreased with respect to those of the TO after the two reaction steps. The characterization of the obtained foams, achieved through the measurement of the characteristic reaction times, thermal and dynamic mechanical analysis, scanning electronic microscopy, and density measurements, is reported and discussed. The most important changes in the modified foams were found with the addition of GLY to the formulation; this led to an increased foam density and storage rubbery modulus, which were associated with a higher crosslinking density because of the decrease in the chain length between crosslinking points.","palabras_clave":"flexible polyurethane|biobased polyols|tung oil"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5009,"idioma_id":4,"titulo":"Relationship between intracellular calcium and morphologic changes in rabbit erythrocytes: Effects of the acylated and unacylated forms of E. coli alpha-hemolysin","resumen":"Alpha-hemolysin (HlyA) is a hemolytic and cytotoxic protein secreted by uropathogenic Escherichia coli strains, whose expression correlates with the severity of the infections produced. HlyA is synthesized as a protoxin, ProHlyA, that becomes matured to the active form in the cytosol by hemolysin-C directed fatty acylation at the &#949;-amino residues of Lys 564 and Lys 690, before export from the toxin-producing bacteria. This posttranslational modification is remarkable because the nature of the protein is changed by the lipidic moiety from a benign protein to a frank toxin. In the present work, we demonstrated for the first time that, despite being  hemolitically inactive, ProHlyA induced cellular morphologic changes in rabbit erythrocytes. A discocyte-to-echinocyte transformation was triggered by the protoxin in the absence of any accompanying increase in intracellular-Ca2+ levels. In addition, the Ca2+ -influx kinetics in HlyA-treated erythrocytes indicated that the active toxin induced an elevation in intraerythrocyte-Ca2+ content in a biphasic manner, with an initial rise in Ca2+ that depended on the association of the protein with the target membrane and a second increment corresponding to an activation of purinergic channels. The first increase was sufficient to trigger adiscocyte-echinocyte-spherocyte transition in erythrocyte shape, though the subsequent rise mediated by purinergic signalling was essential for the occurrence of hemolysis. The results presented here provide new insights into the mechanism of action of this toxin","palabras_clave":"HLYA|CALCIUM|RABBIT ERYTHROCYTES|EXOVESICLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5010,"idioma_id":4,"titulo":"Physiological differences between two overlapped breeding Antarctic penguins in a global change perspective","resumen":"Global change has affected the Antarctic Peninsula influencing the abundance of krill, one of the main prey of penguins. In areas where breeding penguin populations overlap, species with a more diverse diet have generally been less affected than krill-specialist species, which have shown population declines. Human activities can add to these changes, as penguins are sensitive to anthropic impacts such as contamination. Our objective was to assess whether selected physiological parameters of Adélie and Gentoo penguins reflect their contrasting population trends in a colony located at Punta Stranger (25 de Mayo Island\/King George, South Shetland Islands) where they breed sympatrically. During 2012 we assessed the leukocyte profile, heterophil to lymphocyte ratio (H\/L), erythrocytic nuclear abnormalities (ENAs), hematocrit, biochemical profile and a measure of immune function (bacterial agglutination) in adults and chicks of both species. Higher values of ENAs, indicative of genotoxic damage caused by contaminants, are in accordance with a greater sensitivity to ongoing global changes by Adélie penguins. Levels of cholesterol and triglycerides strengthen this idea since individuals could be investing more resources in energy reserves to successfully cope with challenging environmental conditions during the breeding season. The remaining physiological parameters did not provide a clear picture. Furthermore, some results could be related to differences in diet. Gentoo show greater prey diversity than Adélie penguins, incorporating a richer parasite fauna, which could explain their higher heterophils and H\/L. The physiological parameters measured here serve as baseline for a sustained monitoring of these rapidly changing populations. Further physiological variables, including stress hormone and indices of oxidative stress, remain to be assessed as potential indicators of population susceptibility to global change in this system.","palabras_clave":"ANTARCTICA|GLOBAL WARMING| PHYSIOLOGY|PYGOSCELIS PENGUIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5011,"idioma_id":4,"titulo":"Mineral Phosphate Solubilization in  Burkholderia tropica Involves an Inducible  PQQ-Glucose Dehydrogenase","resumen":"Aims: The objective of this work was to provide knowledgement about the mechanism and regulation of the mineral phosphate solubilization in Burkholderia. tropica. To this end, the expression of the direct extracellular oxidative pathway in B. tropica was studied using different culture approaches.Study Design: Plate assays and batch cultures in flasks and bioreactor were carried out in this study with B. tropica Mto-293 like target organism. The experiments were achieved at least three times with two repetitions per time.Place and Duration of Study: Departamento de Química, Centro de Investigación y Desarrollo en Fermentaciones Industriales, UNLP, CCT-La Plata-CONICET, between November 2014-2015.Methodology: Qualitative plate assays with different Carbon sources were carried out for the evaluation of Mineral Phosphate Solubilization phenotype. Batch cultures in flasks were carried out with different Carbon, Phosphorus and Nitrogen sources to determine quantitatively soluble phosphorus, gluconic acid and other ketoacids in the supernatants, and also PQQ-linked glucose and gluconate dehydrogenase activities in whole cells. Cultures with some of the conditions mentioned before were carried out in bioreactor specifically to control pH.Results: This organism was able to produce significant amounts of gluconic acid via the expression of a PQQ-GDH and also showed a significant activity of GaDH. However, the direct oxidative pathway was only observed under conditions of Phosphorus starvation and\/or Nitrogen fixation.Conclusion: The Mineral Phosphate Solubilization phenotype for B. tropica can be ascribed to the expression of the direct oxidative pathway which involves the expression of an active PQQ- linked glucose dehydrogenase. Nevertheless, this pathway is not expressed constitutively in this bacterium. Environmental conditions, like low P and N availability, led to an active extracellular glucose oxidation. Therefore, mineral phosphate solubilization in B. tropica involves an inducible pyrroloquinoline quinone-linked glucose dehydrogenase. These findings may contribute to the use of this bacterium as plant growth promoting bacteria reducing the dependence on chemical fertilizer.","palabras_clave":"Burkholderia tropica|P-solubilization|PQQ|glucose dehydrogenase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5012,"idioma_id":5,"titulo":"Lenguaje racional versus 'calculus ratiocinator' ¿Se aplica esta distinción a Leibniz?","resumen":"El presente trabajo aborda la cuestión acerca de si la distinción o diferencia que establece Frege entre una lingua universales y un calculus ratiocinator puede ser atribuida a Leibniz. La conclusión de nuestros análisis  es que la distinción no puede sostenerse desde un punto de vista terminológico, dado que Leibniz utiliza las expresiones ?characteristica universalis? y ?calculus ratiocinator? con significados que, aunque no sean necesariamente idénticos, se hallan muy próximos entre sí. Sin embargo, puede encontrarse esta diferencia desde un punto de vista conceptual, independientemente de las consideraciones terminológicas. En efecto, cuando se consideran los diversos  modos en que Leibniz define la tarea de la característica como instrumento de pensamiento, surge la necesidad de postular una diferencia entre una característica universal y una característica general. Así, la característica universal corresponde en términos aproximados a lo que Frege denomina ?lingua rationalis? o ?lingua characterica?, mientras que la característica general pertenece al dominio de lo que Frege sostuvo acerca de un ?calculus ratiocinator?, aunque en la concepción de Leibniz la característica general tiene un alcance mucho mayor que la de un cálculo diseñado como un formalismo para nuestras inferencias.","palabras_clave":"Leibniz|Lógica|Lenguaje"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5013,"idioma_id":4,"titulo":"Homogenization and Mass Identity vs. Individual Identity. An Analysis in the Light of the Theory of the ?Three Dimensional Spiral of Sense","resumen":"This study tests some hypotheses included in the psycho-social-communicational paradigm, which emphasizes the cognitive effects of the media and the role of the psychosocial subject as the recipient: the hypothesis of 'agenda-setting' (Cobb y Elder, 1971; McLeod-Becker, 1974; McCombs y Shaw, 1976). It also conforms a new systemic theory of the author called 'The Three Dimensional Spiral of Sense' (2015 d), applied at the area of Media. It is the first study in Latin America. The approach was macro-micro-meso-macro, micro, not quite common yet. It consists of a kind of sui generis systemism which recovers relationships (links, back and forth) between individuals and contexts, without overlooking neither the former nor the latter, thus, avoiding any type of reductionism. Individuals, organizations and frameworks interplay and feedback themselves. The four main objectives were: 1. Determining the degree of impact of the press in the mental patterns et detecting levels de homogenization en university graduates; 2. Analyzing the relation between institutional ideology, political ideology and media choise graduates make (traditional vs more progressive media); in other words, the meso, macro and micro interplay. 3. Ascertaining the personality factors that condition differential receptivity ('Filtering' of the news), related to the social-evaluative context. Methodology used included qualitative and quantitative techniques. The results show: a) a high level of influence of the media on the problems which have been prioritized by graduates; b) a high level of coincidence of the topics prioritized by Faculties (prevailing 'ideologies'); c) individual and institutional homogenization which gets feedback within a macro context of homogenization of the news and of globalization. All this impacts on institutional and individual identities. ","palabras_clave":"Homogenization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5014,"idioma_id":4,"titulo":"Vegetation productivity trends in response to urban dynamics","resumen":"Urbanization is a global phenomenon with still unknown consequences for vegetation dynamics of urban ecosystems, especiallyin subtropical areas of developing countries. In this paper we analyze the vegetation productivity trend associatedto urban densification and urban expansion during the last decade, in twelve cities of northern Argentina. We used timeseries analysis of MODIS-NDVI images to reconstruct the phenological patterns to retrieve a productivity trend underthree spatial classes of urban dynamics: (1) urban, (2) expansion and (3) periphery. Our results show that trends in vegetationproductivity are more associated to the environmental characteristics (basal productivity and climate) than to theland cover class. The average trend in productivity in urban areas ranged between &#8722;2.54% year&#8722;1 (Metán) and &#8722;0.22%year&#8722;1 (Concepción). In contrast, the range was much tighter between classes; it was &#8722;1.37% year&#8722;1 in urban areas and&#8722;1.21% in the periphery. In this sense we found significant differences between cities, but no significant differences wereobserved between classes. Urban growth and urban expansion patterns found in our study suggest the system dynamicsis dominated by sprawl patterns rather than by a homogeneous densification. Related to this phenomenon, our resultsdismissed the idea of urban expansion as the main factor affecting vegetation phenology and supported the hypothesisof regional warming as an explanation for the decrease in vegetation productivity, probably due to the decrease of waterbalance in arid regions.","palabras_clave":"MODIS|TIME SERIES ANALYSIS|URBAN EXPANSION|VEGETATION PRODUCTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5015,"idioma_id":4,"titulo":"Evolution of hepatitis C virus in HIV coinfected patients under antiretroviral therapy","resumen":"Five patients (P) were followed-up for an average of 7.73years after highly active antiretroviral therapy (HAART) initiation. Patients´ immune and virological status were determined by periodical CD4+T-cell counts and HIV and HCV viral load. HCV populations were studied using longitudinal high throughput sequence data obtained in parallel by virological and immunological parameters. Two patients (P7, P28) with sub-optimal responses to HAART presented HCV viral loads significantly higher than those recorded for two patients (P1, P18) that achieved good responses to HAART. Interestingly, HCV populations from P7 and P28 displayed a stable phylogenetic structure, whereas HCV populations from P1 and P18showeda significant increase in their phylogenetic structure, followed by a decrease after achieving acceptable CD4+T-cell counts (>500 cell\/&#956;l). The fifth patient (P25) presented high HCV viral loads, preserved CD4+T-cell counts from baseline and all along the follow-up, and displayed a constant viral phylogenetic structure. These results strongly suggest that HAART-induced immune recovery induces a decrease in HCV viral load and an increase in the HCV population phylogenetic structure likely reflecting the virus diversification in response to the afresh immune response. The relatively low HCV viral load observed in the HAART responder patients suggests that once HCV is adapted it reaches a maximum number of haplotypes higher than that achieved during the initial stages of the immune response as inferred from the two recovering patients. Future studies using larger number of patients are needed to corroborate these hypotheses.","palabras_clave":"Hepatitis C|HIV|Evolution|HAART"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5016,"idioma_id":4,"titulo":"Fluorescent responsive chlorophyllide-hydrogel for carbon dioxide detection","resumen":"The chlorophyllide b derivatized polyallylamine (PAAChl) was synthesized. The resulting fluorescent polymer presents a high emission quantum yield, very stable in time. The polymer formed a hydrogel when treated with carbon dioxide that was characterized by Raman spectroscopy and solid-state NMR (ss-NMR). The adsorption of CO2 takes place through the formation of carbamate. Absorbance and fluorescence based analytical studies were performed. The UV-vis spectra of PAAChl, after additions of CO2, show a growing absorption peak at 250 nm corresponding to carbamate formation. The emission of fluorescence at 664 nm as function of carbon dioxide concentrations shows a linear regression with a detection limit of 2 ppm and a quantification limit of 5 ppm. The capacity of a chemical sensor was described by sensibility, selectivity, stability, response time and regeneration were also analyzed.","palabras_clave":"CO2|FLUORESCENCE|CHLOROPHYLLIDE|SENSOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5017,"idioma_id":4,"titulo":"Global Meteorological Drought:  A Synthesis of Current Understanding with a Focus on SST Drivers of Precipitation Deficits","resumen":"p { margin-bottom: 0.1in; direction: ltr; line-height: 120%; text-align: left; }a:link { color: rgb(0, 0, 255); }a.western:link {  }a.cjk:link {  }a.ctl:link { font-family: 'Times New Roman'; }Drought affects virtually every region of the world, and potential shifts in its character in a changing climate are a major concern. This article presents a synthesis of current understanding of meteorological drought, with a focus on the large-scale controls on precipitation afforded by sea surface temperature (SST) anomalies, land surface feedbacks, and radiative forcings. The synthesis is primarily based on regionally focused articles submitted to the Global Drought Information System (GDIS) collection together with new results from a suite of atmospheric general circulation model experiments intended to integrate those studies into a coherent view of drought worldwide. On interannual time scales, the preeminence of ENSO as a driver of meteorological drought throughout much of the Americas, eastern Asia, Australia, and the Maritime Continent is now well established, whereas in other regions (e.g., Europe, Africa, and India), the response to ENSO is more ephemeral or nonexistent. Northern Eurasia, central Europe, and central and eastern Canada stand out as regions with few SST-forced impacts on precipitation on interannual time scales. Decadal changes in SST appear to be a major factor in the occurrence of long-term drought, as highlighted by apparent impacts on precipitation of the late 1990s ?climate shifts? in the Pacific and Atlantic SST. Key remaining research challenges include (i) better quantification of unforced and forced atmospheric variability as well as land?atmosphere feedbacks, (ii) better understanding of the physical basis for the leading modes of climate variability and their predictability, and (iii) quantification of the relative contributions of internal decadal SST variability and forced climate change to long-term drought.","palabras_clave":"ATM\/OCEAN STRUCTURE\/ PHENOMENA|DROUGHT|PRECIPITATION|SEA SURFACE TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5018,"idioma_id":4,"titulo":"Evaluation of WRF Model Forecasts and Their Use for Hydroclimate Monitoring over Southern South America","resumen":"Weather forecasting and monitoring systems based on regional models are becoming increasingly relevant for decision support in agriculture and water management. This work evaluates the predictive and monitoring capabilities of a system based on WRF model simulations at 15 km grid spacing over La Plata Basin (LPB) in southern South America, where agriculture and water resources are essential. The model´s skill up to a lead-time of 7 days is evaluated with daily precipitation and 2m temperature in-situ observations for the 2-year period 01 August 2012 to 31 July 2014. Results show high prediction performance with 7 days lead-time through-out the domain and particularly over LPB, where about 70% of rain and no-rain days are correctly predicted. Also, the probability of detection of rain days is above 80% in humid regions. Temperature observations and forecasts are highly correlated (r&gt;0.80) while mean absolute errors, even at the maximum lead-time, remain below 2.7°C for minimum and mean temperatures and below 3.7°C for maximum temperatures. The usefulness of WRF products for hydro-climate monitoring was tested for an unprecedented drought in southern Brazil and for a slightly above normal precipitation season in northeastern Argentina. In both cases the model products reproduce the observed precipitation conditions with consistent impacts on soil moisture, evapotranspiration and runoff. This evaluation validates the model´s usefulness to forecast weather up to one week and to monitor climate conditions in real time. The scores suggest that the forecast&nbsp; lead-time can be extended into week two, while bias correction methods can reduce part of the systematic errors.","palabras_clave":"WEATHER FORECASTS|FORECASTS VERIFICATION|HYDROCLIMATE MONITORING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5019,"idioma_id":5,"titulo":"COMPOSICIÓN QUÍMICA PROXIMAL Y PERFIL DE ÁCIDOS GRASOS DEL MEJILLÓN (Mytilus edulis) PROVENIENTES DE CULTIVOS Y BANCOS NATURALES EN EL GOLFO SAN JORGE, ARGENTINA.","resumen":"Se analizó el contenido de carne, la composición química proximal y el perfil de ácidos grasos de la carne de mejillones recolectados en bancos naturales infralitorales y en cultivos localizados en el Golfo San Jorge, Argentina. Se comparó el contenido de humedad, cenizas, lípidos, proteínas y glúcidos. El perfil de ácidos grasos se determinó por cromatografíagaseosa y fue empleado para calcular el índice aterogénico (IA) y el índice trombótico (IT). Los mejillones del banco natural tienen un menor contenido relativo de carne que los ejemplares de cultivo. Se obtuvieron diferencias significativas en la  composición química proximal de los mejillones. La carne de mejillón presentó un alto contenido de humedad, siendo ésta mayor en mejillones de bancos. Las proteínas son el componente bioquímico con mayor porcentaje, seguido por los hidratos de carbono y en menor cantidad por los lípidos. Independientemente del origen de los animales, la carne de mejillón se caracterizó por una mayor cantidad de ácidos grasos polinsaturados que ácidos grasos monoinsaturados y saturados. El IA en mejillones de banco fue levemente menor que en los ejemplares de cultivo, mientras que el IT fue el mismo en ambos casos. Las diferencias observadas en la composición química proximal de los mejillones de cultivos y bancos naturales se deberían al hábitat que ocupan, el cual determina diferencias en la calidad y cantidad de alimentos y diferencias en la energía destinada a mantenimiento.","palabras_clave":"COMPOSICIÓN QUÍMICA|MYTILUS|ACIDOS GRASOS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5020,"idioma_id":4,"titulo":"Effects of chronic forced circadian desynchronization on body weight and metabolism in male mice","resumen":"Metabolic functions are synchronized by the circadian clock setting daily patternsof food intake, nutrient delivery, and behavioral activity. Here, we studythe impact of chronic jet-lag (CJL) on metabolism, and test manipulationsaimed to overcome potential alterations. We recorded weight gain in C57Bl\/6mice under chronic 6 h advances or delays of the light?dark cycle every2 days (ChrA and ChrD, respectively). We have previously reported ChrA, butnot ChrD, to induce forced desynchronization of locomotor activity rhythmsin mice (Casiraghi et al. 2012). Body weight was rapidly increased underChrA, with animals tripling the mean weight gain observed in controls by day10, and doubling it by day 30 (6% vs. 2%, and 15% vs. 7%, respectively). Significantincreases in retroperitoneal and epidydimal adipose tissue masses(172% and 61%, respectively), adipocytes size (28%), and circulating triglycerides(39%) were also detected. Daily patterns of food and water intake wereabolished under ChrA. In contrast, ChrD had no effect on body weight.Wheel-running, housing of animals in groups, and restriction of food availabilityto hours of darkness prevented abnormal increase in body weightunder ChrA. Our findings suggest that the observed alterations under ChrAmay arise either from a direct effect of circadian disruption on metabolism,from desynchronization between feeding and metabolic rhythms, or both.Direction of shifts, timing of feeding episodes, and other reinforcing signalsdeeply affect the outcome of metabolic function under CJL. Such featuresshould be taken into account in further studies of shift working schedules inhumans.","palabras_clave":"Chronic jet-lag,    |circadian disruption,|restricted feeding,|shift work,|wheel-running"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5021,"idioma_id":5,"titulo":"Geología y Geofísica del extremo norte de la sierra de Villa Corral (31º08?LS-69º24?LO), Precordillera occidental de San Juan.","resumen":"En esta contribución se presenta la geología y geofísica del extremo norte de la sierra de Villa Corral. La zona de estudio se desarrolla íntegramente en el ámbito de la Precordillera Occidental de San Juan (31º08´LS - 69º24´LO). Se comparan los aspectos estructurales y estratigráficos de una sección NE-SO levantada a lo largo de la Quebrada del Tigre, con datos aeromagnéticos y de sensoramiento remoto. Se aplicaron cocientes entre bandas en imágenes satelitales Landsat7 ETM+ los cuales resultaron de gran utilidad en las posteriores determinaciones estratigráficas y estructurales. La estructura general de la sierra de Villa Corral está definida por un pliegue sinclinal con eje buzante al SE. Dos sistemas de corrimientos (Andinos y Pre-Andinos) segmentan parcialmente ambos flancos del pliegue principal. Estos corrimientos pueden ser agrupados en función de la orientación, inclinación de los planos, edad relativa y vergencia de la deformación. Los corrimientos Pre-Andinos se orientan NO-SE con planos inclinados al NE y movimiento de techo al SO, mientras que los corrimientos Andinos se disponen N-S con planos inclinados al O y movimiento de techo al E. Las unidades involucradas por ambos sistemas de corrimientos incluyen rocas que varían desde el Eopaleozoico hasta el Neógeno lo cual pone de manifiesto que todas las estructuras han sufrido activaciones tectónicas durante la orogenia Andina. Finalmente, el diseño de las cartas de anomalía magnética residual, señal analítica, Tilt y gradiente horizontal del Tilt guarda estrecha relación con los aspectos estructurales y estratigráficos observados en superficie, lo cual permite inferir de manera ajustada la disposición de las estructuras y unidades geológicas en subsuelo. El perfil de señal analítica muestra la importancia de los contrastes magnéticos producidos por los corrimientos principales al mismo tiempo que permite ajustar la distribución de unidades en subsuelo de acuerdo al desplazamiento relativo de los bloques de falla.","palabras_clave":"ANOMALIA MAGNETICA|SENSORES REMOTOS|GEOLOGIA ESTRUCTURAL|EOPALEOZICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5022,"idioma_id":4,"titulo":"Pericarp-Imposed Dormancy in Sunflower: Physiological Basis, Impact on Crop Emergence, and Removal at an Industrial Scale","resumen":"Sunflower (Helianthus annuus L.) achenes oftendisplay pericarp-imposed dormancy, whichis long-lasting and causes serious problemsto crop production and the seed industry.For this study we assessed an extensivelyused sunflower inbred line that has this typeof dormancy. Our goals were (i) to determinethe effect of pericarp on germination and toevaluate its impact on crop field emergence, (ii)to provide insight into the physiological basis ofpericarp-imposed dormancy by determining theeffects of abscisic acid (ABA) accumulation inthe embryo and the embryo sensitivity to ABAduring incubation at different temperatures, (iii)to assess the effect of oxidant agents and othercompounds on dormancy termination, and(iv) to evaluate the feasibility of using oxidantsto remove dormancy at an industrial scale.Incubation at high temperatures (i.e., 25 to 30°C)allowed the expression of dormancy, which wasimposed by the pericarp and was accompaniedby an increase in embryo sensitivity to ABA,but not in ABA concentration. Treated acheneswith sodium hypochlorite, or their incubation inpresence of an ethylene precursor or gibberellinsovercame dormancy. ABA concentrationdecreased during incubation when treatedwith sodium hypochlorite. Application ofsodium hypochlorite on a commercial seed lot(i.e., washing with 3 and 7%, after additionalchemicals used by the industry were applied)resulted in higher germination compared withdormant non-treated controls. Field trialsshowed that pericarp-imposed dormancyreduced crop emergence in the inbred linetested herein. However, treating achenes withsodium hypochlorite using described industrialprocedures improved field emergence.","palabras_clave":"Heliantus  annuus|dormancy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5023,"idioma_id":4,"titulo":"Phylogenetic relationships of toads of the Rhinella granulosa group (Anura: Bufonidae): a molecular perspective with comments on hybridization and introgression","resumen":"The Rhinella granulosa group consists of 13 species of toads distributed throughout open areas of South America and Panama.In this paper we perform a phylogenetic analysis considering all but one species of the group, employing five nuclear andfour mitochondrial genes, for up to 7910 bp per specimen. Separate phylogenetic analyses under direct optimization (DO) ofnuclear and mitochondrial sequences recovered the R. granulosa group as monophyletic and revealed topological incongruencethat can be explained mainly by multiple events of hybridization and introgression, both mitochondrial and nuclear. The DOcombined analysis, after the exclusion of putatively introgressed or heterozygous genomes, resulted in a phylogenetic hypothesisfor the R. granulosa group in which most of the species are recovered as monophyletic, but with interspecific relationshipspoorly supported. The optimization of morphological (adult and larval), chromosomal, and behavioural characters resulted in12 putative phenotypic synapomorphies for this species group and some other synapomorphies for internal clades. Our resultsindicate the need for additional population genetic studies on R. dorbignyi and R. fernandezae to corroborate the taxonomic statusof both taxa. Finally, we discuss biological and genetic characteristics of Bufonidae, as possible explanations for the commonoccurrence of hybridization and introgression observed in some lineages of this family.","palabras_clave":"Anura|Bufonidae|Rhinella"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5024,"idioma_id":4,"titulo":"Sheep grazing and soil bacterial diversity in shrublands of the Patagonian Monte, Argentina","resumen":"Our objective was to assess whether long-term continuous sheep grazing was associated with the bacterial community of the Patagonian Monte soils. We randomly extracted soil samples from 5 plant covered patches (PCP) and the nearest inter-canopy areas (IC) at sites with low (L), moderate (M) andheavy (H) grazing intensity. Bacterial communities were evaluated by denaturing gradient gel electrophoresis (DGGE) and dominant bands were sequenced. Dendrograms showed a different association among grazing sites in PCP compared to IC areas. In PCP, M and H sites were clustered and DGGE revealeda high similarity in the bacterial community regardless of grazing intensity. In IC areas, analyses clustered L and M sites. Band sequencing revealed that members of these communities belonged to Sphingobacteria,Solibacteres, Gammaproteobacteria, Betaproteobacteria and Bacilli. Some of the band sequencesrelated to Nitrosococcus oceani and Nitrosococcus watsonii which are restricted to marine environments and salt lakes. Microcoleus vaginatus and diatom chloroplast-related sequences were mainly detected in IC areas from L and M. Heavy grazing could alter autotrophic bacteria\/diatom composition in IC areaspossibly due to physical disturbance of biological crusts by trampling, threatening the potential of crust re-formation. Grazing intensity affected soil bacterial communities differently in PCP and IC areas with soil crust, highlighting the importance of improving our understanding of microbial diversity responses to land use.","palabras_clave":"ARID ECOSYSTEMS|GRAZING|BACTERIA|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5025,"idioma_id":4,"titulo":"Anatomical study of the auditory region of Arctotherium tarijense (Ursidae, Tremarctinae), an extinct short-faced bear from the Pleistocene of South America","resumen":"Here we present the most detailed morphological study of the auditory region of a tremarctinae bear,Arctotherium tarijense Ameghino. In addition, we provide new anatomical information of the Tremarctinaeinner ear, such as coplanarity and deviation from orthogonality of the semicircular canals, as an approachto infer the head movements which encountered the extinct forms in locomotion. Based on morphologicalcomparisons, A. tarijense exhibits the following particular features: the cavum tympani presents the highestrelative volume compared with other ursids; the processus paraoccipitalis has a foramen that is absent inother tremarctines; there is only one (ventral) recess in the anterior region of the cavum tympani; and therecessus epytimpanicus is the smallest for all ursids studied. In relation to the inner ear, A. tarijense showsthe lowest values of orthogonality deviation and highest scores of locomotor agility. Based on this, ispossible to make a preliminary proposal that this species had a relative high vestibular sensibility andtherefore a better ability to explore different kind of habitats. However, this hypothesis might becontrasted among bears taking into account the orientation of each semicircular canal in a phylogeneticframework.","palabras_clave":"Arctotherium|morphology|auditory region|locomotor agility"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5026,"idioma_id":4,"titulo":"Approximate expressions of a SBR for wastewater treatment: Comparison with numeric solutions and application to predict the biomass concentration in real cases","resumen":"Most industrial wastewater treatment systems often operate under transient conditions, causing several operational problems. An effective solution is the use of Sequencing Batch Reactors (SBR). In general, a great number of simulations are necessary to solve SBRs mathematical models in order to evaluate the effect of the operational conditions on the performance of the reactor. In this work, a set of analytical equations that represent the effect of the operational parameters on the performance of a SBR was developed. The obtained equations adequately represent the change of the organic substrate, ammonia, biomass, oxygen and soluble microbial products as a function of time within a single operation cycle of the SBR. The equations also predict the steady-state concentrations as a function of several operational parameters, avoiding the problem of performing a great number of simulations. Based on real SBR data, the biomass growth yield and the decay factor for two synthetic wastewaters were obtained. Using these coefficients, the proposed equations adequately predicted the biomass concentration in real cases.","palabras_clave":"SEQUENCING BATCH REACTOR|MODELLING|STEADY-STATE|CHEESE WHEY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5027,"idioma_id":4,"titulo":"Rab GTPases and the Autophagy Pathway: Bacterial Targets for a Suitable Biogenesis and Trafficking of Their Own Vacuoles","resumen":"Autophagy is an intracellular process that comprises degradation of damaged organelles, protein aggregates and intracellular pathogens, having an important role in controlling the fate of invading microorganisms. Intracellular pathogens are internalized by professional and non-professional phagocytes, localizing in compartments called phagosomes. To degrade the internalized microorganism, the microbial phagosome matures by fusion events with early and late endosomal compartments and lysosomes, a process that is regulated by Rab GTPases. Interestingly, in order to survive and replicate in the phagosome, some pathogens employ different strategies to manipulate vesicular traffic, inhibiting phagolysosomal biogenesis (e.g., Staphylococcus aureus and Mycobacterium tuberculosis) or surviving in acidic compartments and forming replicative vacuoles (e.g., Coxiella burnetti and Legionella pneumophila). The bacteria described in this review often use secretion systems to control the host?s response and thus disseminate. To date, eight types of secretion systems (Type I to Type VIII) are known. Some of these systems are used by bacteria to translocate pathogenic proteins into the host cell and regulate replicative vacuole formation, apoptosis, cytokine responses, and autophagy. Herein, we have focused on how bacteria manipulate small Rab GTPases to control many of these processes. The growing knowledge in this field may facilitate the development of new treatments or contribute to the prevention of these types of bacterial infections.","palabras_clave":"RAB GTPASES|AUTOPHAGY|BACTERIAL PATHOGENS|INTRACELLULAR BACETRIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5028,"idioma_id":5,"titulo":"Condiciones metamórficas en la faja máfica-ultramáfica de Precordillera: nuevas perspectivas en el vínculo entre Precordillera y Cordillera Frontal","resumen":"LA FAJA MÁFICA-ULTRAMÁFICA DE LA PRECORDILLERA PUEDE DIVIDIRSE EN DOS SECTORES:CENTRO-NORTE Y SUR. ESTE ÚLTIMO SE LOCALIZA EN LA PRECORDILLERA MENDOCINA Y SE CARACTERIZA POR LA PRESENCIA DE SERPENTINITAS Y GRANULITAS MÁFICAS RETROGRADADAS, EN CONTACTO CON METASEDIMENTITAS Y CUERPOS METABASÁLTICOS. LAS GRANULITAS MÁFICAS DEL SECTOR SUR DESCRIBEN UNA TRAYECTORIA METAMÓRFICA ANTIHORARIA: HAN SIDO SOMETIDAS A UN METAMORFISMO DE ALTA PRESIÓN Y TEMPERATURA, Y LUEGO RETROGRADADAS A FACIES ESQUISTOS VERDES. EN TANTO, LAS METASEDIMENTITAS Y METABASALTOS ALCANZARON LA FACIES DE ESQUISTOS VERDES DE ALTA PRESIÓN.ALGUNOS AUTORES CONSIDERAN AL ÁREA DE GUARGUARÁZ, CORDILLERA FRONTAL, COMO LA CULMINACIÓN AUSTRAL DE ESTA FAJA. ADEMÁS DE LAS SIMILITUDES ENTRE EL SECTOR SUR DE LA MISMA Y ESTA ÁREA EN CUANTO A LA ASOCIAICÓN MÁFICA-ULTRAMÁFICA, MAGMATISMO Y EDADES, SE DESTACAN SEMEJANZAS EN LAS CONDICIONES DE ALTA PRESIÓN, TRAYECTORIAS ANTIHORARIAS Y GRADIENTES METAMÓRFICOS. ESTO PERMITE RATIFICAR UNA CORRELACIÓN ASOCIADA A LA EVOLUCIÓN DE CUYANIA Y CHILENIA.","palabras_clave":"GRANULITAS|ESQUISTOS VERDES|ALTA PRESIÓN|CUYANIA|CHILENIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5029,"idioma_id":5,"titulo":"ASPECTOS ESTRATIGRÁFICOS DE  LA FORMACIÓN ALCAPARROSA EN  LA PRECORDILLERA OCCIDENTAL  SANJUANINA (31o20?LS -69o20?LO).  INTEGRACIÓN DE IMÁGENES  SATELITALES Y GEOLOGÍA DE  CAMPO","resumen":"Se presentan los resultados del estudio geológico de la faja de rocas máficasy metasedimentarias asociadas (Formación Alcaparrosa), de la Precordillera Occidental sanjuanina (31º20?LS -69º20?LO). La aplicación de sensores remotos ha permitido caracterizar la distribución areal de los diferentes cuerpos de roca que integran a esta unidad y ordenarlos estratigráficamente en función de la posición que ocupan dentro de la estructura geológica general. La estratigrafíade la Formación Alcaparrosa puede ser dividida en dos miembros que de base a techo son: Miembro Arenoso, representado por una alternancia de bancos de areniscamedia y fina que se habrían depositado en un paleoambiente marino de talud externo de profundidad moderada; Miembro Lutítico, caracterizado por intercalaciones de lutitas verdes y moradas que representa la sedimentaciónhemipelágica. Diques gábricos y filones capa de diabasa, se alojan exclusivamente en los niveles del Miembro Arenosos,mientras que, lavas almohadilladas se intercalan entre las hemipelagitas del Miembro Lutítico.","palabras_clave":"Precordillera Occidental|Sensores remotos|Faja ofiolítica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5030,"idioma_id":4,"titulo":"Continuous moderate grazing management promotes biomass production in Patagonian arid rangelands","resumen":"Domestic grazing effects on primary productivity and community structure are controversial in rangelandecology and frequently misunderstood. Although directly related with secondary production,biomass stock and biomass production at species level (biomass composition) has been relegated in fieldstudies, especially in arid rangelands co-dominated by woody species. We estimated grazing effects onaboveground biomass in a temperate mixed grass-shrub steppe of Patagonia. We compared exclusion ofsheep with two levels of continuous grazing: moderate (light) and intensive sheep grazing in an averageprecipitation year. Total green biomass (productivity) was twice as high in moderately grazed paddocksas in those without grazing and intensively grazed pastures, while standing dead grass biomass stockonly decreased in intensive grazing. Shrub biomass was not modified by grazing management. Inaddition, grazing modified grass specific biomass composition, thus diminishing biomass quality inintensively grazed areas. This work provides evidence that in arid rangelands, continuous moderategrazing management could be an effective tool to increase productivity compared to grazing exclusion.Furthermore, moderate grazing would not cause major undesired changes in species composition.However, a potential risk of land use intensification exists because intensive grazing could decreasebiomass production as well as promote negative composition changes.","palabras_clave":"Domestic herbivores|Grasses|Grazing optimization process|Primary productivity|Shrubs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5031,"idioma_id":4,"titulo":"High Risk of Metabolic and Adipose Tissue Dysfunctions in Adult Male Progeny, Due to Prenatal and Adulthood Malnutrition Induced by Fructose Rich Diet","resumen":"The aim of this work was to determine the effect of a fructose rich diet (FRD) consumed by the pregnant mother on the endocrine-metabolic and in vivo and in vitro adipose tissue (AT) functions of the male offspring in adulthood. At 60 days of age, rats born to FRD-fed mothers (F) showed impaired glucose tolerance after glucose overload and high circulating levels of leptin (LEP). Despite the diminished mass of retroperitoneal AT, this tissue was characterized by enhanced LEP gene expression, and hypertrophic adipocytes secreting in vitro larger amounts of LEP. Analyses of stromal vascular fraction composition by flow cytometry revealed a reduced number of adipocyte precursor cells. Additionally, 60 day-old control (C) and F male rats were subjected to control diet (CC and FC animals) or FRD (CF and FF rats) for three weeks. FF animals were heavier and consumed more calories. Their metabolic-endocrine parameters were aggravated; they developed severe hyperglycemia, hypertriglyceridemia, hyperleptinemia and augmented AT mass with hypertrophic adipocytes. Our study highlights that manipulation of maternal diet induced an offspring phenotype mainly imprinted with a severely unhealthy adipogenic process with undesirable endocrine-metabolic consequences, putting them at high risk for developing a diabetic state.","palabras_clave":"metabolic programming;    |metabolic syndrome|adipocyte precursor cells;|dysfunctional adipose tissue;|altered glucose tolerance;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5032,"idioma_id":4,"titulo":"Towards efficient biocatalysts: photo-immobilization of a lipase on novel lysozyme amyloid-like nanofibrils","resumen":"Herein, we report the preparation and characterization of a new biocatalyst based on the photo-immobilization of a lipase onto hybrid protein:sugar nanofibrils. The nanofibrils are obtained through the aggregation of hen white egg lysozyme induced by the highly sulfated glycosaminoglycan heparin. The new hybrid nanomaterial could be easily functionalized using photochemical reaction to attach lipases through dityrosine covalent bonds. Compared to the free enzyme, the photo-immobilized lipase has better thermostability and enhanced resistence to non-conventional environment. Structural and morphological characterization of the nanofibrils shows that they are compatible with amyloid-like aggregates. In addition, the supramolecular arrangement of heparin and lysozyme within the building unit of the nanofibrils is also proposed. The procedure reported herein could be useful to design a new generation of insoluble biocatalyst by a singlephoto-click step in a way that appears cleaner and faster than conventional chemical cross-linked procedures.","palabras_clave":"nanofibril|photoimmobilization|biocatalysis|phospholipase|biodiesel"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5033,"idioma_id":4,"titulo":"Long-Term Fructose Intake Increases Adipogenic Potential: Evidence of Direct Effects of Fructose on Adipocyte Precursor Cells","resumen":"Abstract: Wehavepreviouslyaddressedthatfructoserichdiet(FRD)intakeforthreeweeksincreases the adipogenic potential of stromal vascular fraction cells from the retroperitoneal adipose tissue (RPAT). We have now evaluated the effect of prolonged FRD intake (eight weeks) on metabolic parameters, number of adipocyte precursor cells (APCs) and in vitro adipogenic potential from control (CTR) and FRD adult male rats. Additionally, we have examined the direct fructose effects on the adipogenic capacity of normal APCs. FRD fed rats had increased plasma levels of insulin, triglyceride and leptin, and RPAT mass and adipocyte size. FACS studies showed higher APCs number and adipogenic potential in FRD RPAT pads; data is supported by high mRNA levels of competency markers: PPAR&#947;2 and Zfp423. Complementary in vitro experiments indicate that fructose-exposed normal APCs displayed an overall increased adipogenic capacity. We conclude that the RPAT mass expansion observed in eight week-FRD fed rats depends on combined accelerated adipogenesis and adipocyte hypertrophy, partially due to a direct effect of fructose on APCs.","palabras_clave":"retroperitoneal adipose tissue;\t\t\t|adipogenesis;|SVF cells;|precursor cell competency"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5034,"idioma_id":4,"titulo":"Characterization of novel human papillomavirus types 157, 158 and 205 from healthy skin and recombination analysis in genus &#947;-Papillomavirus","resumen":"Gammapapillomavirus (&#947;-PV) is a diverse and rapidly expanding genus, currently consisting of 79 fully characterized human PV (HPV) types. In this study, three novel types, HPV157, HPV158 and HPV205, obtained from healthy sun-exposed skin of two immunocompetent individuals, were amplified by the ?Hanging droplet? long PCR technique, cloned, sequenced and characterized. HPV157, HPV158 and HPV205 genomes comprise 7154-bp, 7192-bp and 7298-bp, respectively, and contain four early (E1, E2, E6 and E7) and two late genes (L1 and L2). Phylogenetic analysis of the L1 ORF placed all novel types within the &#947;-PV genus: HPV157 was classified as a new member of species &#947;-12 while HPV158 and HPV205 belong to species &#947;-1. We then explored potential recombination events in genus &#947;-PV with the RDP4 program in a dataset of 74 viruses (71 HPV types with available full-length genomes and the 3 novel types). Two events, both located in the E1 ORF, met the inclusion criterion (p-values b0.05 with at least four methods) and persisted in different ORF combinations: an inter-species recombination in species &#947;-8 (major and minor parents: species &#947;-24 and &#947;-11, respectively), and an intraspecies recombination in species &#947;-7 (recombinant strain: HPV170; major and minor parents: HPV-109 and HPV-149, respectively). These findings were confirmed by phylogenetic tree incongruence analysis. An additional incongruence was found in members of species &#947;-9 but it was not detected by the RDP4. This report expands our knowledge of the family Papillomaviridae and provides for the first time in silico evidence of recombination in genus &#947;-PV.","palabras_clave":"NOVEL HUMAN PAPILLOMAVIRUS|HPV157|HPV158|HPV205|GENUS GAMMA-PV|RECOMBINATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5035,"idioma_id":4,"titulo":"Evaluation of food grade antioxidant formulation for sustained antifungal, antiaflatoxigenic and insecticidal activities on peanut conditioned at different water activities","resumen":"The aim of this study was to investigate antifungal and insecticidal activity of two microencapsulated antioxidants: 2(3)-tert-butyl-4 hydroxyanisole (BHA) and 2,6-di(tert-butyl)-p-cresol (BHT) against Aspergillus section Flavi and Oryzaephilus surinamensis (L.), a vector carrier of aflatoxigenic fungi on stored peanuts. Susceptibility of Aspergillus section Flavi, insects, and aflatoxin B1 accumulation in sterile peanut kernels conditioned at two different water activities (aw) (0.83 aw and 0.95 aw) was determined with different doses of antioxidant formulations (10, 20 and 30 mM) during 45 days. Moreover, Aspergillus section Flavi isolation frequency from live and dead insects was evaluated. The BHA formulation completely inhibited Aspergillus section Flavi development regardless of aw and doses assayed. Antifungal effect of microencapsulated BHT was highly dependent on aw, with 86-100% fungal inhibition at 20 and 30 mM, at the lowest aw (0.83 aw) and at the end of the experiment. No aflatoxin accumulation was detected in samples treated with the BHA formulation. In general, low levels of Aspergillus section Flavi were detected in dead insects. Our results show efficacy for 45 days, in addition microencapsulated BHT could be an alternative to control peanut pests in dry kernels.","palabras_clave":"Aspergillus section Flavi|Aflatoxin B1|Butylated hydroxyanisole (BHA)|Butylated hydroxytoluene (BHT)|Oryzaephilus surinamensis (L.)|Peanut"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5036,"idioma_id":5,"titulo":"Políticas en Salud Mental y constitución de subjetividad en la niñez.","resumen":"En la ponencia se presentaron los resultados de la investigación realizada en el marco del Programa Postdoctoral. El objetivo de la misma es analizar el impacto de las Políticas en Salud Mental, las legislaciones y las prácticas de los actores sociales en la producción de subjetividad en la niñez.Las preguntas que guiaron la investigación fueron ¿Cuáles son los efectos que las políticas y los nuevos marcos normativos tienen en las prácticas institucionales que abordan los problemas de salud mental en la niñez? y ¿De qué modo éstos impactan en los proceso de constitución subjetiva de los niños y las niñas?Se trata de una investigación que se llevó a cabo a través de un diseño exploratorio descriptivo, enmarcado en un abordaje de tipo cualitativo. Los procedimientos para la recolección de datos comprendieron un estudio documental y revisión exhaustiva de la información sobre la temática, la realización de entrevistas a informantes claves y en profundidad a profesionales que trabajan en las instituciones de salud y en los organismos de protección de derechos. Se centró en el período temporal establecido entre 2010 y 2015 en la Ciudad de Buenos Aires, Argentina.El aporte que se propone la investigación está dado por el desarrollo de un análisis conceptual volcado a la visibilización de las problemáticas de salud mental en la niñez; y la producción de conocimientos que puedan facilitar la toma de decisiones y mejorar los procesos de implementación de nuevas políticas y prácticas en Salud Mental, orientadas a evitar las desigualdades y mejorar la calidad de las intervenciones y las condiciones de vida en la niñez.Entre los resultados más relevantes se detectaron importantes tensiones entre los marcos normativos con enfoque de derechos y las prácticas institucionales decuidados de salud mental. La dificultad para replantear políticas en el campo de la Salud favoreció, por parte de las instituciones sanitarias, la profundización de los procesos de medicalización y patologización de niños y niñas con sufrimiento psicosocial que habitan en condiciones adversas.Los discursos institucionales vehiculizados a través de los actores sociales produjeron formas específicas de prácticas en Salud Mental y establecieron un modo particular de constituir subjetividades y crear identidades desacreditadas y estigmatizadas.La estructura de poder de la autoridad médica y discursos psi. se instalaron conviolencia sobre los cuerpos de niñas y niños y el valor performativo de los diagnósticos dejó afuera condiciones reales de existencia.Se visibilizó una creciente institucionalización psiquiátrica en la niñez, y lanaturalización de las violencias en dichas instituciones. Violencias que atraviesan las vivencias de los niños y niñas en el marco de instituciones totales donde se implementan contenciones físicas y químicas, salas de aislamiento, restricciones arbitrarias al contacto con familiares y uso excesivo y\/o indebido de la medicación. En este marco, la complicidad estructural de los servicios, contribuyó a reproducir las desigualdades sociales, mientras que distintas voces de niños y niñas de diferentes modos reclamaban por sus derechos eran silenciadas.","palabras_clave":"Niñez|Derechos|Políticas |Subjetividades"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5037,"idioma_id":5,"titulo":"PAUTAS DE DISEÑO PARA DISMINUIR LAS TEMPERATURAS URBANAS EN REGIONES CON ALTA PRODUCTIVIDAD SOLAR. PARÁMETROS MORFOLÓGICOS Y MATERIALES","resumen":"El presente estudio examinó el potencial de las características del perfil urbano morfología y materialidad para disminuir las temperaturas urbanas. El objetivo es identificar pautas de diseño y rehabilitación urbana, que permitan densificar áreas residenciales de la ciudad de Mendoza, Argentina. Para ello fueron simulados 32 escenarios con el programa ENVI-met 3.1, combinando distintos niveles de reflectancia solar (albedo) de los materiales que conforman las envolventes, morfologías de perfil urbano, y la presencia o no de forestación dentro del canal vial, variando su orientación E-O y N-S. A partir de los resultados obtenidos de las simulaciones, se infiere que aquellos canales viales que presentan un comportamiento térmico más eficiente, están vegetados, y su perfil urbano morfológicamente es continuo sobre línea municipal. En cuanto a la materialidad de sus envolventes presentan niveles de albedo altos (0.7) en techos y pavimentos, y niveles de albedo bajo (0.3) en fachadas.","palabras_clave":"Diseño Urbano|Fachadas|Materiales|Morfología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5038,"idioma_id":4,"titulo":"Synthesis of enantiomeric polyhydroxyalkylpyrrolidines from 1,3-dipolar cycloadducts. Evaluation as inhibitors of a  &#946;-galactofuranosidase","resumen":"Enantiomeric 2,3,4-tris(hydroxyalkyl)-5-phenylpyrrolidines have been synthesized from the major cycloadducts obtained by the 1,3-dipolar cycloaddition of sugar enones with azomethine ylides derived from natural amino acids. Reduction of the ketone carbonyl group of the cycloadducts, which possess a basic structure of bicyclic 6-menthyloxyhexahydropyrano[4,3-c]pyrrol-7(6H)one, afforded a number of pyrrolidine-based bicyclic systems. A sequence of reactions, which involved hydrolysis of the menthyloxy substituent, reduction, N-protection and degradative oxidation, afforded varied pyrrolidine structures having diverse configurations and patterns of substitution, particularly polyhydroxylated derivatives have been obtained. The unprotected products were isolated as pyrrolidinium trifluoroacetates. Because of the furanose-like nature of the target trihydroxyalkyl pyrrolidines, these molecules have been evaluated as inhibitors of the beta-galactofuranosidase from Penicillium fellutanum. The compounds showed practically no inhibitory activity for concentration of pyrrolidines in the range of 0.1 to 1.6 mM.","palabras_clave":"CARBOHYDRATE|PYRROLIDINE|CYCLOADDITION|GLYCOMIMETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5039,"idioma_id":4,"titulo":"Nano-supramolecular complex synthesis: switch on\/off enhanced fluorescence control an  molecular release using a simple chemistry reaction","resumen":"A nanosensor based on &#946;-cyclodextrin (&#946;CD) macrocycles linked to gold nanoparticles for rhodamine B (RhB)sensing was developed applying the metal-enhanced fluorescence effect (MEF). Hence, we have developed many ways to control the distance of supramolecular systems to nanoparticle surface with different bioconjugation strategies in order to optimize signal detection. Different PEG spacer arm lengths were used to cover the nanoparticle surface with molecular spacers. This type of molecular shell is biocompatible, enabling to switch on\/off the MEF effect using a dithiane linker by a simple reduction reaction. In the presence of the nanosensor obtained, an increase was observed in RhB fluorescence emission depending on molecular length, that is a characteristic effect ofMEF. Themajor increasemeasuredwas 60% comparedwith RhB emission in buffer at 1 nMlevel, for a spacer length of 3.58 nm and an 80% increase as compared with that in the presence of &#946;CD. These differences are ascribed to the fact that, in the presence of macrocycle, we can observe a well-known quenching effect that is overcome by the presence of the metallic core. Even at shorter spacer distances, with PEG lengths of 1.2 and 2.17nm, increases of 25 and 47% respectively allowthe analyte detection by the RhB complexation with &#946;CD. The optimal MEF enhancement was measured with the maximal emission signal stabilized after 50 min due to plasmonic effects based on inter nanoparticle interactions. Moreover, the emission increase, in the presence of the metallic core, was accompanied with a diminution in the fluorescence lifetime´s decay value averages, characteristic of MEF. This fact shows that the excited state is protected from the non-radiative emission decays enhancing the analytical signal.","palabras_clave":"Nanosupramolecular|Luminiscence|Switch on\/off|Drug delivery"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5041,"idioma_id":4,"titulo":"Broad resistance to acetohydroxyacid-synthase-inhibiting herbicides in feral radish (Raphanus sativus L.) populations from Argentina","resumen":"BACKGROUND: Soon after the commercial release of sunflower cultivars resistant to imidazolinone herbicides, several uncontrolled feral radish (Raphanus sativus L.) populations were found in south-eastern Buenos Aires, Argentina. These populations were studied in field, glasshouse and laboratory experiments aiming to characterise their resistance profile and to developmanagement tools.RESULTS: Three feral radish accessions were highly resistant to ten active ingredients of five families of acetohydroxyacid synthase (AHAS)-inhibiting herbicides. Sequence analysis of the AHAS gene detected a Trp574Leu mutation in all resistant accessions. One accession with an intermediate level of resistance was heterozygous for this mutation, probably owing to gene exchange with a susceptible subpopulation located in the field margin. Herbicide-resistant and herbicide-susceptible radish could be controlled in sunflower by alternative herbicides.CONCLUSION: This is the first report of feral radish with resistance to herbicides belonging to all the AHAS-inhibiting herbicide families, conferred by Trp574Leu mutation in theAHASgene.An appropriate herbicide rotation with alternative herbicides such as fluorochloridone or aclonifenandanincrease inthediversityof cropping systems are important for minimising the prevalence of these biotypes.","palabras_clave":"RAPHANUS SATIVUS|FERAL RADISH|HERBICIDE-RESISTANCE|AHAS|IMAZETHAPYR|METSULFURON"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5042,"idioma_id":4,"titulo":"Evaluation of different heterologous prime-boost immunization strategies against Babesia bovis using viral vectored and protein-adjuvant vaccines based on a chimeric multi-antigen","resumen":"Protection against the intraerythrocytic bovine parasite Babesia bovis requires both humoral and cellular immune responses. Therefore, tailored combinations of immunogens targeted at both arms of the immune system are strategies of choice to pursue sterilizing immunity. In this study, different heterologous prime-boost vaccination schemes were evaluated in mice to compare the immunogenicity induced by a recombinant adenovirus, a modified vaccinia Ankara vector or a subunit vaccine all expressing a chimeric multi-antigen. This multi-antigen includes the immunodominant B and T cell epitopes of three B. bovis proteins: Merozoite Surface Antigen - 2c (MSA-2c), Rhoptry Associated Protein - 1 (RAP-1) and Heat Shock Protein 20 (HSP20). Both priming with the adenovirus or recombinant multi-antigen and boosting with the modified vaccinia Ankara vector achieved a high degree of activation of TNF&#945; and IFN&#947;-secreting CD4(+) and CD8(+) specific T cells 60days after the first immunization. High titers of specific IgG antibodies were also detected at the same time point and lasted up to day 120 of the first immunization. Only the adenovirus - MVA combination triggered a marked isotype skew for the IgG2a antibody subclass meanwhile for the other immune traits analyzed here, both vaccination schemes showed similar performances. The immunological characterization in the murine model of these rationally designed immunogens led us to propose that adenoviruses as well as the bacterially expressed multi-antigen are highly reliable primer candidates to be considered in future experiments in cattle to test protection against bovine babesiosis.","palabras_clave":"MVA|Adenovirus|Vacuna|Babesia bovis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5043,"idioma_id":4,"titulo":"Namuncura´ Marine Protected Area: an oceanic hot spot of benthic biodiversity at Burdwood Bank, Argentina","resumen":"The first open-sea (non-coastal) Marine Protected Area (MPA) in Argentina was created in 2013 (and named ?Namuncurá?), at Burdwood Bank, an undersea plateau located about 200 km south from Malvinas\/Falkland Islands, SW Atlantic Ocean. This contribution represents the most recent and complete checklist of benthic organisms (mainly mega and macrofauna) that summarized 240 taxa collected at three sites in the mentioned area. The benthic richness here documented largely exceeds the values coming from other productive areas in Argentinean waters. Faunistic composition was different among the sites, although several species were shared. Bryozoans presented the highest richness compared to the other groups, followed by mollusks and sponges. These data represent valuable information and a very helpful starting point for incoming research, conservation efforts and management. It is essential to establish the spatial distribution of indicator taxa, like corals, sponges and bryozoans, to better attempt at the protection of benthic biodiversity in this rich Argentinean marine system. This is a key issue considering the notable extension of the MPA compared to other areas in Argentina, the bathymetric variations, particular oceanographic conditions and different fishing circumstances within the area.","palabras_clave":"Hot spot|Benthic communities|New Marine Protected Area|SW Atlantic Ocean"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5044,"idioma_id":4,"titulo":"Scleractinian corals recorded in the Argentinean Antarctic expeditions between 2012 and 2014, with comments on Flabellum (Flabellum) areum Cairns, 1982","resumen":"In this contribution, we provide a list of the scleractinian corals recorded during the Argentinean Antarctic expeditions on board the oceanographic vessel Puerto Deseado (Argentina) in the austral summers in 2012, 2013 and 2014. Theidentified taxa consist of six solitary species (Flabellum impensum, F. flexuosum, F. areum, Caryophyllia antarctica, Paraconotrochus antarcticus and Javania antarctica), recorded from 19 sampling sites located off the Antarctic Peninsula and South Shetland and South Orkney islands.We also update the information of F. areum, previously known only from south-west Atlantic waters, extending itsdistribution range to Antarctic waters and its upper bathymetric range to 218 m","palabras_clave":"COLD WATER CORALS|ANTARCTICA|SCLERACTINIA|DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5045,"idioma_id":5,"titulo":"Diversidad de las comunidades bentónicas en las regiones de reclutamiento de la merluza común Merluccius hubbsi Marini, 1933","resumen":"Las características ambientales de las áreas de reclutamiento y cría de peces juegan un rol clave en su supervivencia. En este contexto, el objetivo del trabajo fue caracterizar la diversidad de las comunidades macrobentónicas asociadas a los prerreclutas de la merluza común en la región del Golfo San Jorge (?Plataforma Patagónica?, PP) y en la Zona Común de Pesca Argentino-Uruguaya (ZCPAU). Se utilizó información proveniente de la captura incidental de las campañas de evaluación del INIDEP (2005- 2011). Se encontraron 147 taxones en la PP y 165 en la ZCPAU, dominados por crustáceos, moluscos y equinodermos. Los estimadores de riqueza asintótica total para cada región variaron entre 154-184 (PP) y 182-214 (ZCPAU). PP presentó una densidad de especies más baja que ZCPAU, así como una riqueza menor para todos los valores de individuos considerados. Sin embargo, varias de sus asociaciones bentónicas se caracterizaron por una mayor presencia de especies que aumentan la complejidad del sustrato, incrementando el refugio de los prerreclutas ante la depredación, y por ende una mayor supervivencia. Esta hipótesis deberá ser evaluada a largo plazo con estudios dirigidos específicamente al bentos, aprovechando la línea de base generada a partir de los muestreos de captura incidental.","palabras_clave":"MERLUZA COMUN|PESQUERIAS|CAPTURA INCIDENTAL|DIVERSIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5046,"idioma_id":4,"titulo":"Contrasting effects of fire frequency on plant traits of three dominant perennial herbs from Chaco Serrano","resumen":"Fire frequencies are currently increasing inmany regions across the world as a result of anthropic activities,affecting ecological processes and plant population dynamics. Fire can generate important changes in soil properties,altering nutrient dynamics and thereby plant growth. Here we analyse fire frequency effects on soil quality and planttraits of three native perennial herbaceous plants (Cologania broussonetii, Desmodium uncinatum and Rhynchosia edulis;Fabaceae) with the capacity for biologicalN2 fixation that resprouts and is abundant after fire in Chaco Serrano forests.Based on 22-year fire history, we assessed plant traits in sites with low and high fire frequencies along with unburnedscenarios. We found significantly lower water content, nitrates and electrical conductivity in frequently burned soils.As a result, the three species showed consistently lower leaf area and specific leaf area in both fire frequencies, implyinglower growth rates in comparison to unburned sites. However, total leaf biomass was not affected by fire, leaf phosphorusconcentration was higher in R. edulis in high fire frequency and leaf N concentration was twice as large in plantsgrowing in sites of high fire frequency in C. broussonetii and R. edulis. Such an increase in N and phosphorus concentrationsis likely a result of both their conservative use of resources and their biologicalN2 fixation capacity. To our knowledge,this is the first record of such contrasting fire effects observed consistently in three co-occurring species: whileplant growth decreased with fire frequency, leaf nutritional traits remain unchanged or increased in frequently burnedsites. Quality-depleted and drier soils that result fromincreased fire frequenciesmay not only affect trait variation at theintraspecific level but can also drive to a homogenization of the plant community, selecting species with particular combinationsof morphological and functional traits.","palabras_clave":"FIRE REGIME|BIOLOGICAL N2 FIXATION|LEAF NUTRIENT|SPECIFIC LEAF AREA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5047,"idioma_id":4,"titulo":"Simple Synthesis of Aldehyde and Carboxylic Acid -Terminated Methoxypoly(ethylene glycol)","resumen":"mPEG-aldehydes and activated mPEG-carboxylic acids on which the polymer and the reactive functional group are separated by aliphatic chains of different lengths, are well known reagents of widespread application in PEGylation technology. The syntheses of these functional polymers are not easy and usually the preparation of each of these compounds requires a very specific set of reagents and experimental conditions. In fact, a general method for their preparation is, still nowadays, unavailable. In this work we develop a simple synthetic method for preparation of mPEG-aldehydes and carboxylic acids with excellent yields. The key step of the method involves a substitution reaction of mPEG-mesylate with mono-alkoxides of symmetrical diols to afford hydroxyl terminated polymers, which are converted to the aldehydes and acids by mild oxidation reactions.","palabras_clave":"PEGylation|Bioconjugation|Activated Polymers|PEG aldehydes and Acids"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5048,"idioma_id":4,"titulo":"Palynological evidence of the geoecological belts dynamics from Eastern Cordillera of NW Argentina (23° S) during the Pre-Last Glacial Maximum","resumen":"This paper presents the first palynologycal evidences that provide paleoenvironmental data of the geoecological belts of the Eastern Cordillera in Northwestern Argentina during the millennia prior to the Last Glacial Maximum (LGM). The study was carried out on a 5-m deep sedimentary core raised at Laguna Blanca (23°09?S, 65°12?W; 4256 masl), province of Jujuy. The geochronology was based on three C14 dating by AMS method. Results show the sensitive response of the geoecological belts to temperature and effective moisture variations from ca. 29,000 cal years BP to ca. 25,000 cal years BP. The High Andean belt probably suffered an altitudinal descent forced by intense cold climate conditions ca. 29,000 cal years BP, and may have reached a distribution comparable to the present one on 26,300 cal years BP, favored by a less cold and dry climate than the former phase. The Puna belt did not suffer great changes, since it remained with low representation in the pollen spectrum. A noteworthy regional biogeographic aspect is the presence of high pollen percentages of the Yungas arboreal species ca. 29,000 cal years BP. Probably this is a response to the intensification of the anabatic winds that ascend the slope of the Sierras Subandinas towards the Eastern Cordillera. These results represent an important contribution to the paleoenvironmental discussion of the Upper Pleistocene for the Argentine Northwestern region, where the available information on vegetation history is scarce.","palabras_clave":"Eastern Cordillera|Geoecologycal belts.|Pre Last Glacial Maximum|Palynology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5049,"idioma_id":4,"titulo":"Regarding the real diversity of Glyptodontidae (Mammalia, Xenarthra) in the late Pliocene (Chapadmalalan Age\/Stage) of Argentina","resumen":"A large diversity of Glyptodontidae has been proposed as characterizing the Chapadmalalan Age (Pliocene). Most of these taxa were recognized on the basis of partial dorsal carapaces and\/or caudal tubes, whereas the main diagnostic character is a particular morphology of the exposed surface of the osteoderms. From a biostratigraphic view point some species have importance. The Upper Chapadmalalan is based on the Paraglyptodon chapadmalensis biozone. Both the re-evaluation of the type and referred materials and new significant findings from the Chapadmalal and El Polvorín Formations indicate that the diversity of Pliocene Glyptodontidae is more limited than previously supposed. The particular morphology of the exposed surface of the osteoderms that characterizes some of the species actually corresponds to a taphonomic alteration, which results in a non-real ornamentation pattern. Thus, the Glyptodontinae P. chapadmalensis must be replaced as a fossil guide because this species is not well characterized, as well as the species included in the genera Urotherium, Trachycalyptus and Lomaphorus. Taking into account the diversity of Glyptodontidae for this lapse, the Glyptodontinae are very scarce (a situation that contrasts with its records in the Pleistocene), whereas Eosclerocalyptus, ?Plohophorini? (Plohophorus) and Doedicurinae (cf. Eleutherocercus antiquus) are among the most recorded taxa","palabras_clave":"Diversity|Glyptodonts|Osteoderms|Pliocene"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5050,"idioma_id":4,"titulo":"Eigenvalue homogenisation problem with indefinite weights","resumen":"In this work we study the homogenization problem for nonlinear elliptic equations  involving $p-$Laplacian type operators with sign changing weights. We study the  asymptotic behavior of variational eigenvalues,  which consist on a double sequence of eigenvalues. We show that the $k-$th positive eigenvalue goes to infinity when the average of the weight is nonpositive, and converge to the $k-$th variational eigenvalue of the limit problem when the average is positive for any $kge 1$.","palabras_clave":"eigenvalues|homegenization|quasilinear problems|indefinite weights"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5051,"idioma_id":4,"titulo":"Out of the forest: past and present range expansion of a parthenogenetic weevil pest, or how to colonise the world successfully","resumen":"Previous research revealed complex diversification patterns in the parthenogenetic weevil Naupactus cervinus. To understand the origin of clonal diversity and successful spreading of this weevil, we investigated its geographic origin and possible dispersal routes and whether parthenogens can persist in habitats under unsuitable environmental conditions. This study is based on samples taken throughout a broad area of the species? range. We used both mitochondrial and nuclear markers and applied phylogenetic and network analyses to infer possible relationships between haplotypes. Bayesian phylogeographic analyses and ecological niche modeling were used to investigate the processes that shaped genetic diversity and enabled the colonization of new geographic areas.Southeastern Brazil emerges as the original distribution area of N. cervinus. Wedetected two range expansions, one along natural corridors during the Pleis-tocene and the other in countries outside South America during recent times.Isolation due to climate shifts during the early Pleistocene led to diversification in two divergent clades, which probably survived in different refugia of the Paranaense Forest and the Parana River delta. The origin of the clonal diversity was probably a complex process including mutational diversification, hybridization, and secondary colonization. The establishment of N. cervinus in areas outside its native range may indicate adaptation to drier and cooler conditions. Parthenogenesis would be advantageous for the colonization of new environments by preventing the breakup of successful gene combinations. As in other insect pests, the present distribution of N. cervinus results from both its evolutionary history and its recent history related to human activities.","palabras_clave":"ECOLOGICAL NICHE MODELING|INVASIVE WEEVILS|NAUPACTUS CERVINUS|PARTHENOGENESIS|PLEISTOCENE REFUGIA|RANGE EXPANSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5052,"idioma_id":4,"titulo":"An inhomogeneous singular perturbation problem for the $p(x)$-Laplacian","resumen":"In this paper we study the following singular perturbation problem for the $p_\\ep(x)$-Laplacian:\\begin{equation}\\label{eq}\\tag{$P_\\ep(\\fep, p_\\ep)$}\\Delta_{p_\\ep(x)}\\uep:=\\mbox{div}(|\\nabla \\uep(x)|^{p_\\ep(x)-2}\\nabla\\uep)={\\beta}_{\\varepsilon}(\\uep)+\\fep, \\quad u^{\\ep}\\geq 0,\\end{equation} where $\\ep>0$,${\\beta}_{\\varepsilon}(s)={1 \\over \\varepsilon} \\beta({s \\over\\varepsilon})$, with $\\beta$  a  Lipschitz  function satisfying$\\beta>0$ in $(0,1)$, $\\beta\\equiv 0$ outside $(0,1)$ and $\\int\\beta(s)\\, ds=M$. The functions $\\uep$, $\\fep$ and $p_\\ep$ are uniformly bounded.We prove uniform Lipschitz regularity, we pass to the limit $(\\ep\\to 0)$ and we show that, under  suitable assumptions, limit functions are weak solutions to the free boundary problem: $u\\ge0$ and\\begin{equation}\\label{fbp-px}\\tag{$P(f,p,{\\lambda}^*)$}\\begin{cases}\\Delta_{p(x)}u= f & \\mbox{in }\\{u>0\\}\\\\u=0,\\ |\\nabla u| = \\lambda^*(x) & \\mbox{on }\\partial\\{u>0\\}\\end{cases}\\end{equation}with $\\lambda^*(x)=\\Big(\\frac{p(x)}{p(x)-1}\\,M\\Big)^{1\/p(x)}$,$p=\\lim p_\\ep$ and $f=\\lim \\fep$.In \\cite{LW4} we prove that the free boundary of a weak solution is a $C^{1,\\alpha}$ surface near flat free boundary points. This result applies, in particular, to the limit functions studied in this paper.","palabras_clave":"FREE BOUNDARY PROBLEM|VARIABLE EXPONENT SPACES|SINGULAR PERTURBATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5053,"idioma_id":4,"titulo":"Bone shell microstructure of Condorchelys antiqua Sterli, 2008, a stem turtle from the Jurassic of Patagonia","resumen":"tThe histology of the turtle shell has proven to be a valuable source of characters for thestudy of development and shell origin, as well as early turtles? lifestyles and systematics.Here we describe and discuss the shell bone microanatomy and histology of Condorchelysantiqua, a stem turtle from the Lower Jurassic of Patagonia. Examination of several elements(including costals, neurals, peripherals and plastral plates) reveals that the shell bones con-sist of a diploe structure in which the interior area of cancellous bone is framed by externaland internal cortices. Whereas the external cortex is mostly composed of structural fiberbundles, histological composition of the internal cortex is more variable, exhibiting struc-tural fiber bundles and\/or parallel fibered bone. This histological variation in the internalcortex allows discerning between different types of plates. The cancellous bone is mostlywell developed and consists of fine trabeculae composed of secondary lamellar bone tissue.Predominance of structural fiber bundles in the shell bones suggests that metaplasia playsan important role during the development of the shell in this taxon. Comparison with otherstem Testudines (including Proganochelys quenstedti, Proterochersis robusta, Kayentachelyssp., Eileanchelys waldmani, and Heckerochelys romani) reveals that the shell histology ofC. antiqua resembles more H. romani and E. waldmani than the other stem taxa. The obtainedvalues of compactness of C. antiqua shell bones suggest an aquatic lifestyle for this taxon.","palabras_clave":"Stem Testudines|Microanatomy|Shell Histology|Lifestyle"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5054,"idioma_id":4,"titulo":"Auxiliary Inductances Design of a Bidirectional Three-Port DC-DC Converter","resumen":"This paper proposes a methodology to design the auxiliary inductances of a bidirectional three-port DC-DC converter, which allows minimize the currents through the high frequency transformer and switches. In this way, RMS current and also the current values at the switching instants, as function of auxiliary inductances, are analyzed considering the different operating conditions. From this analysis, design considerations for these inductances are determined. Finally, experimental results are presented to validate the theory.","palabras_clave":"THREE PORT DC-DC CONVERTER|HYBRID ELECTRIC SYSTEM|POWER LOSSES |AUXILIARY INDUCTANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5055,"idioma_id":4,"titulo":"A Gamma convergence approach to the critical Sobolev embedding in variable exponent spaces","resumen":"In this paper, we study the critical Sobolev embeddingsW1,p(x)(&#937;)&#8834;Lp&#8727;(x)(&#937;) for variable exponent Sobolevspaces from the point of view of the &#915;-convergence. More precisely wedetermine the &#915;-limit of subcritical approximation of the best constantassociated with this embedding. As an application we provide a sufficientcondition for the existence of extremals for the best constant.","palabras_clave":"gamma convergence |variable exponents |sobolev embeddings"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5056,"idioma_id":5,"titulo":"Al rescate de la niñez indígena. Reflexiones antropológicas a partir de una campaña de UNICEF Argentina","resumen":"En este trabajo nos proponemos analizar, desde una perspectiva antropológica, la ?Campaña por los derechos de la niñez y la adolescencia indígena? lanzada por UNICEF Argentina en 2009, destinada a hacer visibles 'las vulneraciones específicas de derechos que sufre esta población en Argentina' y a 'sensibilizar y lograr la movilización ciudadana'. Distanciándonos de concepciones ontológicas sobre los derechos humanos, abordamos esta Campaña en tanto dispositivo cuyo efecto es la construcción de los niños indígenas como sujetos de derechos de maneras específicas. Para ello, indagamos en el modo en que fue ésta elaborada, así como en sus contenidos, procurando explicar qué derechos y qué representaciones de los niños indígenas se visibilizaron a través de ella y las tensiones suscitadas en su proceso de armado y presentación.","palabras_clave":"DERECHOS HUMANOS|NIÑEZ|PUEBLOS INDÍGENAS|ORGANISMOS INTERNACIONALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5057,"idioma_id":4,"titulo":"Molecular View of the Interaction of S-Methyl Methanethiosulfonate with DPPC Bilayer","resumen":"We presentmolecular dynamics (MD) simulation studies of the interaction of a chemopreventive and protective agent, S-methyl methanethiosulfonate (MMTS), with amodel bilayer of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine(DPPC). We analyzed and compared its diffusion mechanisms with the relatedmolecule dimethyl sulfoxide (DMSO). We obtainedspatially resolved free energy profiles of MMTS partition into a DPPC bilayerin the liquid-crystalline phase through Potential of Mean Force (PMF)calculations using an umbrella sampling technique. These profiles showed aminimum for MMTS close to the carbonyl region of DPPC. The location of MMTSmolecules in the DPPC bilayer observed in the MD were confirmed by previousSERS studies.1 We decomposed PMF profiles into entropic andenthalpic contributions. These results showed that the driving force for thepartitioning of MMTS into the upper region of DPPC is driven by a favorableentropy change while partitioning into the acyl chains is driven by enthalpy. Onthe other hand, the partition of DMSO into the membrane is not favored, and isdriven by entropy instead of enthalpy. Free diffusion MD simulations using allatom and coarse grained (CG) models of DPPC in presence of MMTS were used toanalyze the effect of DPPC-MMTS interaction. Density profiles showed that MMTSlocates preferentially in the carbonyl region, as expected according to the PMFprofile and the experimental evidence. MMTSpresented two differential effects over the packing of DPPChydrocarbonate chains at low or at high molar ratios. An ordering effect wasobserved when a CG MMTS model was used. Finally, free diffusion MD and PMFdecomposition for DMSO were used for comparison. ","palabras_clave":"S-METHYL METHANETHIOSULFONATE|MOLECULAR DYNAMICS SIMULATION|DPPC|PMF"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5058,"idioma_id":4,"titulo":"Layered Transition Metal Nitroprussides - Their Preparation, Crystal Structure, and Magnetic Properties","resumen":"Divalent transition metal (T) nitroprussides form a series of insoluble coordination compounds, which generally crystallize in 3D frameworks, except for a layered (2D) phase of copper. In this contribution, the preparation of layered phases for T = Mn, Fe, Co, Ni, Zn, and Cd is reported. The incorporation of imidazole (Im) during their preparation by the precipitation method inhibits the coordination of the axial CN groups to the metal T, and layered (2D) structures of formula unit T(Im)2[Fe(CN)5NO] result. The metal T is found coordinated to the N end of the equatorial CN groups. Imidazole molecules occupy its axial coordination sites. In the interlayer region, imidazole molecules from neighboring layers interact through their dipole and quadrupole moments. The stacking of the aromatic rings from neighboring molecules deviates from the sandwich-type configuration, which in turn weakens the magnetic interactions between T2+ cations located in neighboring layers. Axial unbridged CN and NO groups play a steric hindrance role, thus causing a deviation from the sandwich configuration for the aromatic rings. This series of 2D transition metal nitroprussides can be exfoliated by using appropriate solvents, for example, 1-methyl-2-pyrrolidone.","palabras_clave":"LAYERED COMPOUNDS|INTERMOLECULAR INTERACTIONS|MAGNETIC PROPERTIES|COORDINATION MODES|METAL NITROPRUSSIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5059,"idioma_id":4,"titulo":"Layered vanadyl (IV) nitroprusside: Magnetic interaction through a network of hydrogen bonds","resumen":"The hydrogen bond and &#960;-&#960; stacking are two non-covalent interactions able to support cooperative magnetic ordering between paramagnetic centers. This contribution reports the crystal structure and related magnetic properties for VO[Fe(CN)5NO]·2H2O, which has a layered structure. This solid crystallizes with an orthorhombic unit cell, in the Pna21 space group, with cell parameters a=14.1804(2), b=10.4935(1), c=7.1722(8) Å and four molecules per unit cell (Z=4). Its crystal structure was solved and refined from powder X-ray diffraction data. Neighboring layers remain linked through a network of hydrogen bonds involving a water molecule coordinated to the axial position for the V atom and the unbridged axial NO and CN ligands. An uncoordinated water molecule is found forming a triple bridge between these last two ligands and the coordinated water molecule. The magnetic measurements, recorded down to 2 K, shows a ferromagnetic interaction between V atoms located at neighboring layers, with a Curie-Weiss constant of 3.14 K. Such ferromagnetic behavior was interpreted as resulting from a superexchange interaction through the network of strong OH····OH2O, OH····NCN, and OH····ONO hydrogen bonds that connects neighboring layers. The interaction within the layer must be of antiferromagnetic nature and it was detected close to 2 K.","palabras_clave":"NITROPRUSSIDE|MOLECULAR MAGNETISM|HYDROGEN BONDS|LAYERED SOLID|VANADYL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5060,"idioma_id":5,"titulo":"Los Crocodylia (Mesoeucrocodylia, Neosuchia) de la Argentina: estado actual de su registro fósil","resumen":"Herewe present an update of the fossil record of Crocodylia of Argentina, which is representedby Caimaninae alligatorids and Gavialoidea.Less diverse, gavialoids are recorded by a single species, Gryposuchusneogaeus, from the late Miocene of Northeast Argentina. On the other hand, alligatoroids recorded two important moments in its naturalhistory, one at the beginning of the Paleogene (Late Paleocene?Middle Eocene)and the other in the Neogene (Late Miocene). The most ancient record of alligatoroids in South America comesfrom the Early Paleocene of Patagonia. It includes basal forms of caimaninessuch as Necrosuchus ionensis, Eocaiman palaeocenicus, Eocaimancavernensis, and probably a new species of alligatoroid, which provide keymorphological information on the evolutionary and biogeographic history ofthese crocodylians. Anotherimportant moment of the evolutionary history of caimanines is thediversification of the lineage observed during the Miocene. Although there issome isolated cranial material of thisgroup from the Late Miocene of northwestern Argentina, the most abundant anddiverse fossil material comes fromthe northeast, from a level called ?Conglomerado Osífero?. Two genera ofcaimanines are known from this stratigraphic level (Caiman and Mourasuchus)with at least five valid species. ","palabras_clave":"CROCODYLIA|ALLIGATOROIDEA|GAVIALOIDEA|CENOZOICO|ARGENTINA|AMERICA DEL SUR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5061,"idioma_id":4,"titulo":"Agrobacterium-mediated insertional mutagenesis  in the mycorrhizal fungus Laccaria bicolor","resumen":"Agrobacterium-mediated gene transfer (AMT) is extensively employed as a tool in fungal functional genomics and accordingly, in previous studies we used AMT on a dikaryotic strain of the ectomycorrhizal basidiomycete Laccaria bicolor. The interest in this fungus derives from its capacity to establish a symbiosis with tree roots, thereby playing a major role in nutrient cycling of forest ecosystems. The ectomycorrhizal symbiosis is a highly complex interaction involving many genes from both partners. To advance in the functional characterization of fungal genes, AMT was used on a monokaryotic L. bicolor. A collection of over 1200 transgenic strains was produced, of which 200 randomly selected strains were analyzed for their genomic T-DNA insertion patterns. By means of insertional mutagenesis, a number of transgenic strains were obtained displaying differential growth features. Moreover, mating with a compatible strain resulted in dikaryons that retained altered phenotypic features of the transgenic monokaryon. The analysis of the T-DNA integration pattern revealed mostly similar results to those reported in earlier studies, confirming the usefulness of AMT on different genetic backgrounds of L. bicolor. Taken together, our studies display the great versatility and potentiality of AMT as a tool for the genetic characterization of L. bicolor.","palabras_clave":"Agrobacterium|Laccaria|Mycorrhiza|Insertional mutagenesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5062,"idioma_id":4,"titulo":"Thiazine dyes: evaluation of monomeric and aggregate forms","resumen":"The aggregation phenomenon of Azure B, monobrominated Azure B, Thionine and Methylene Blue was studied by UV?Visible spectrophotometry in different media as a function of dye concentration and temperature variations. The tests carried out in organic solvents allowed the identification of monomeric species of these compounds, which have not been reported in literature and have been wrongly assigned for years. The results obtained in water allowed demonstrating that different kinds of aggregates are present in this medium. In addition, the aggregation tendency of these dyes in organic solvent and aqueous media was established. Several parameters such as lipophilicity, effect of bulky substituents and interactions with media were considered to interpret the aggregation behavior of thiazine dyes.","palabras_clave":"Monomer|Dimer |Azure B  |Monobrominated Azure B"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5063,"idioma_id":4,"titulo":"In-situ high-pressure x-ray diffraction study of zinc ferrite nanoparticles","resumen":"We have studied the high-pressure structural behavior of zinc ferrite (ZnFe2O4) nanoparticles by powder X-ray diffraction measurements up to 47 GPa. We found that the cubic spinel structure of ZnFe2O4 remains up to 33 GPa and a phase transition is induced beyond this pressure. The high-pressure phase is indexed to an orthorhombic CaMn2O4-type structure. Upon decompression the low- and high-pressure phases coexist. The  Compressibility of both structures was also investigated. We have observed that the lattice parameters of the high-pressure phase behave anisotropically upon compression. Further, we predict possible phase transition around 55 GPa. For comparison, we also studied the compression behavior of magnetite (Fe3O4) nanoparticles by X-ray diffraction up to 23 GPa. Spinel-type ZnFe2O4 and Fe3O4 nanoparticles have a bulk modulus of 172(20) GPa and 152(9) GPa, respectively. This indicates that in both cases the nanoparticles do not undergo a Hall-Petch strengthening.","palabras_clave":"OXIDES|NANOSTRUCTURE|X-RAY DIFFRACTION|CRYSTAL STRUCTURE|PHASE TRANSITIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5064,"idioma_id":4,"titulo":"The role of metallothioneins, selenium and transfer to offspring in mercury detoxification in Franciscana dolphins (Pontoporia blainvillei)","resumen":"The concentration of mercury (Hg), selenium (Se) and metallothioneins (MT) were evaluated in fetuses, calves, juveniles and adults of the endangered coastal Franciscana dolphin (Pontoporia blainvillei) from Argentina. Mercury concentrations varied among analyzed tissues (liver, kidney, muscle and brain), with liver showing the higher concentrations in all specimens. An age-dependent accumulation was found in liver, kidney and brain. No significant relationship between Hg and MT concentrations was found for all tissues analyzed. Hepatic Hg molar concentrations were positively correlated with those of Se, indicating a great affinity between these two elements. Furthermore, dark granules of HgSe were observed in Kupffer cells in the liver by electron microscopy, suggesting the role of this macrophage in the detoxification of Hg. A transfer of Hg through placenta was proved. The presence of Hg in brain in all age classes did not show concentrations associated with neurotoxicity.","palabras_clave":"MERCURY DETOXIFICATION|PONTOPORIA BLAINVILLEI|METALLOTHIONEINS|SELENIUM|KUPPFER CELL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5065,"idioma_id":4,"titulo":"Nitrification and aerobic denitrification in anoxic-aerobic sequencing batch reactor","resumen":"The aim of this study was to evaluate the feasibility of achieving nitrogen (N) removal using a lab-scale sequencing batch reactor (SBR) exposed to anoxic\/aerobic (AN\/OX) phases, focusing to achieve aerobic denitrification. This process will minimize emissions of N2O greenhouse gas. The effects of different operating parameters on the reactor performance were studied: cycle duration, AN\/OX ratio, pH, dissolved oxygen concentration (DOC), and organic load. The highest inorganic N removal (NiR), close to 70%, was obtained at pH = 7.5, low organic load (440 mgCOD\/(L day)) and high aeration given by 12 h cycle,AN\/OX ratio = 0.5:1.0 and DOC higher than 4.0 mgO2\/L. Nitrification followed by high-rate aerobic denitrification took place during the aerobic phase. Aerobic denitrification could be attributed to Tetrad forming organisms (TFOs) with phenotype of glycogen accumulating organisms using polyhydroxyalkanoate and\/or glycogen storage. The proposed AN\/OX system constitutes an eco-friendly N removal process providing N2 as the end product.","palabras_clave":"NITROGEN|SEQUENCING BATCH REACTOR|ACTIVATED SLUDGE|NITRIFICATION|DENITRIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5066,"idioma_id":4,"titulo":"A quantitative model for lateral flow assays","resumen":"A simple mathematical model that quantitatively describes the dynamics of analyte capture in lateral flow assays is presented. The formulation accounts for the capillary-driven flow through the porous membrane, the advective transport of analyte, and the immunoreactions that takes place in the detection line. Model predictions match the numerical results obtained by computer simulations of the full advection-diffusion-reaction problem in the operating regime of lateral flow assays. The main system parameters were condensed into two dimensionless numbers, namely the relative fluid velocity and the relative analyte concentration. The system is then completely characterized in the space of these criticalnumbers. The model is also able to describe the time evolution of analyte binding by using alternative time scalings, which discriminate different experimental conditions. The equations reported are practical tools for the design and optimization lateral flow tests, enabling informed decisions on basic questions such as the appropriate flow rate, sample volume, or assay time. Beyond lateral flow assays, the work offers an improved understanding of the underlying physico-chemical processes involved in paper-based microfluidics.","palabras_clave":"LATERAL FLOW ANALYSIS|OPTIMAL DESIGN|IMMUNOASSAYS|NUMERICAL SIMULATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5067,"idioma_id":4,"titulo":"In vitro propagation of Lippia integrifolia (Griseb.) Hier. and detection of genetic instability through ISSR markers of in vitro-cultured plants","resumen":"Aromatic and medicinal plants have been traditionally harvested from the wild and, in Argentina, they have beenexploited without any major limitation. In vitro plant propagation is an easy and inexpensive method to obtainhuge amounts of plants in a short period. This practice is relevant for the propagation of valuable and endangeredspecies, facilitating their conservation and germplasm breeding. Since tissue culture can induce genetic andphenotypic variations, collectively termed ?somaclonal variation?, molecular markers have been used to determinethe genetic variability induced. In this work, successful propagation of Lippia integrifolia (Gr.) Hie (?incayuyo?)was achieved with 2,2 &#956;M of bencylaminopurine added to the Murashige-Skoog medium. Shoots were recoveredfrom the development of axillary meristems and through de novo shoots regeneration from organogenic calluses.Regenerated plants were taken to an experimental trial nearby their natural habitat for their evaluation in anagricultural environment. From the moment phenotypic variants started to appear in the ex vitro regeneratedplants, the genetic variability of both kinds of recovered materials was studied by intersimple sequence repeats.Thirteen markers were used, detecting polymorphisms with all the primers tested in both types of recoveredplants. The existence of polymorphisms implies that the genetic stability must be evaluated in all the ex vitrorecovered plants. The protocol developed here is the first step to be applied in biotechnological techniquesemployed to improve the quality of ?incayuyo?. Moreover, this is the first work employing molecular markers in L.integrifolia","palabras_clave":"Tissue culture|BAP|ISSR|somaclonal variation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5068,"idioma_id":4,"titulo":"High-resolution images above the Pampean flat slab of Argentina (31-32oS) from local receiver functions: Implications on regional tectonics","resumen":"In the flat slab region of the South Central Andes (~31-32oS),geological observations suggest that the regional crustal structure isinherited from the accretion of different terranes during the Ordovician.These structures were later reactivated, first in extension during theTriassic and later in compression during the Andean uplift since theMiocene. Seismological observations confirmed that those fault structuresextend to depth with décollement levels that accommodate current crustalshortening in the region. In order to get better insight on the regionaltectonics we computed higher frequency receiver functions from local slabseismicity of intermediate ~100 km depth. Using a common conversion point(CCP) stacking method we obtained cross sections showing high verticalresolution crustal structure at the transition between the Precordilleraand the Frontal Cordillera. In addition we performed a joint inversion ofour high frequency receiver functions with surface wave data from ambientnoise tomography allowing us to constrain absolute seismic wavevelocities. Our higher resolution images reveal more structural detailsdown to a depth of 80 km and laterally over the flat slab in goodagreement with previous studies. Our results help to better identify veryshallow discontinuities in seismic velocities. Recent petrologicalanalyses combined with our RF high-resolution structure correlates with acrustal mafic composition and partial eclogitization in the lower crust.We observe a shift in the crustal structure between the Precordillera andthe Frontal Cordillera. Regional seismicity and previously determinedfocal mechanisms superimposed over our images indicate this shifting is athrust structure extending down to a depth of 40 km. Our results combinedwith petrological analyses suggest the presence of a master décollementbetween the Cuyania (Western Precordillera) and Chilenia (FrontalCordillera) terranes that probably accommodates the deformation in thePampean flat slab region since the Late Ordovician.","palabras_clave":"Terrane|andean retroarc|Body waves|Surface waves"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5069,"idioma_id":4,"titulo":"Combined effect of larval and pupal parasitoid use for Anastrepha fraterculus (Diptera: Tephritidae) control","resumen":"Anastrepha fraterculus (Wiedemann) is a serious pest of commercial fruit production in Argentina. Consequently, biological control is being taken into consideration as a key component of ongoing area-wide fruit fly management strategies. Two parasitoid species are currently considered for mass production and augmentative releases against pest species in the genus Anastrepha in the Americas: the braconid Diachasmimorpha longicaudata (Ashmead), a larval-prepupal koinobiont endoparasitoid, and the diapriid Coptera haywardi (Ogloblin), a native idiobiont pupal endoparasitoid. The prediction that the combined use of D. longicaudata and C. haywardi would be more efficient at suppressing A. fraterculus populations than using them individually was tested under natural environmental conditions. Particularly, the efficacy of both parasitoid species to kill their host, the proportion of superparasitism and theeffect of intrinsic competition on effectiveness of host control were determined. Females of both parasitoids were singly and sequentially released inside a field cage. Peaches artificially inoculated with A. fraterculus larvae were exposed to D. longicaudata, whereas A. fraterculus pupae inside Petri dishes were exposed to C. haywardi. While used separately, effectiveness rates of D. longicaudata and C. haywardi were around 75% and 56%, respectively.However, the total efficacy increased to 93% when they were used sequentially. Coptera haywardi was able to attack hosts that had escaped to D. longicaudata parasitism, contributing by around 19% of the total parasitism in A. fraterculus. Both parasitoid species would induce host mortality through superparasitism. The simultaneous use of both parasitoids in fruit-growing regions for the biological control of A. fraterculus in Argentina is recommended.","palabras_clave":"SOUTH AMERICAN FRUIT FLY|DIACHASMIMORPHA LONGICAUDATA|COPTERA HAYWARDI|MULTIPARASITISM|HOST MORTALITY|ADDITIVE EFFECT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5070,"idioma_id":4,"titulo":"Changes in Diachasmimorpha longicaudata (Hymenoptera: Braconidae) functional response as a consequence of host density choice","resumen":"Most predator and parasitoid functional response studies have been carried out by using experimental designswhere insects are con&#64257;ned to an arena and subsequently exposed to different host densities, which are evaluatedindividually. In the case of a parasitoid that looks for pro&#64257;table patches, this design forces it to use the singlehost density patch available, and therefore the possibility of selection by the parasitoid is not considered atall. A selective functional response, in which the host is distributed in discrete patches at different densities,could be a solution to avoid such a limitation. However, the disadvantage of this design is that it does not meetthe independence assumption required to perform a parametric statistical analysis. Nevertheless, the use ofnonparametric analyses such as GAM and GAMM models allows the performance of this kind of design, makingthe relationship between the response and the explanatory variable more &#64258;exible, looking for general behavioralpatterns. The behavior of the fruit &#64258;y parasitoid Diachasmimorpha longicaudata (Ashmead) in apatched condition was assessed in order to demonstrate that nonparametric analyses are useful tools whenstudying the selective functional response. Results showed that the functional response changed from a ?sigmoidcurve? to a ?bell-shape curve? when the parasitoid had the chance to choose freely among different hostdensities. The female parasitoid distributed their ovarian load among the eight host densities. The present studysuggests that the bell-shape curve displays a general behavior pattern of the parasitoid population.","palabras_clave":"Functional response|Diachasmimorpha longicaudata|Density host preference"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5071,"idioma_id":4,"titulo":"THE CAPILLA DEL MONTE PLUTON, SIERRAS DE CÓRDOBA, ARGENTINA: THE EASTERNMOST EARLY CARBONIFEROUS MAGMATISM IN THE PRE&#8209;ANDEAN SW GONDWANA MARGIN","resumen":"New geochronological, geochemical, and isotopic data are reported for the Capilla del Monte two-mica granite pluton in the northeastern Sierras de Córdoba. An Early Carboniferous age is established by a U?Pb zircon  concordia (336 ± 3 Ma) and a Rb?Sr whole-rock isochron (337 ± 2 Ma). Zircon saturation geothermometry indicates relatively high temperatures (735?800 °C). The granites have high average SiO2 (74.2 %), Na2O + K2O (7.8 %), and high field-strength elements, high K2O\/Na2O (1.7) and FeO\/MgO ratios (5.1), with low CaO content (0.71 %). REE patterns with marked negative Eu anomalies (Eu\/Eu* 0.14?0.56) indicate crystal fractionation, dominantly of plagioclase and K-feldspar, from a peraluminous magma enriched in F. Isotope data (87Sr\/86Srinitial = 0.7086, &#949;Nd336 = &#8722;5.5 to &#8722;4.4 with TDM = 1.5 Ga, zircon &#949;Hf336 +0.8 to &#8722;6.1; mean  TDM = 1.5 Ga) suggest a Mesoproterozoic continental source, albeit with some younger or more juvenile material indicated by the Hf data. The pluton is the easternmost member of a Carboniferous A-type magmatic suite which  shows an increase in juvenile input toward the west in this part of the pre-Andean margin. The petrological and geochemical data strongly suggest a similar intraplate geodynamic setting to that of the nearby but much larger, Late  Devonian, Achala batholith, although Hf isotope signatures of zircon suggest a more uniformly crustal origin for the latter. Further studies are required to understand whether these bodies represent two independent magmatic episodes  or more continuous activity.","palabras_clave":"Geochronological|Carboniferous A-type granites|Pre-Andean marginGeochemistry · Isotopes|Geochemistry|Isotopes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5072,"idioma_id":4,"titulo":"Communication interference in sympatrically occurring moth species","resumen":"In moth species, females emit a species-specific sex pheromone that is perceived over long distance by conspecific males. The species-specificity in the chemical communication channel is achieved by a combination of unique components in specific ratios and sometimes also by interspecific behavioural antagonists to deter sympatrically occurring heterospecific males. In this study, we determined possible antagonistic effects in Helicoverpa gelotopoeon Dyar (Lepidoptera: Noctuidae) males to the major sex pheromone component of sympatrically occurring heliothine moths, Z11-16:Ald, as well as to the sex pheromone of the sympatrically occurring Heliothis virescens (Fabricius) (Lepidoptera: Noctuidae)(Z11-16:Ald and Z9-14:Ald). We also explored whether other co-occurring species are attracted to these pheromone blends. Our field experiments showed that the addition of Z11-16:Ald alone or in combination with Z9-14:Ald inhibited trap catches of H. gelotopoeon males and that this inhibition depended on the concentration of these compounds. In addition, other moth species wereattracted to the blends. Together, our results confirm the antagonistic effect of heterospecific sex pheromone compounds of H. virescens to H. gelotopoeon.","palabras_clave":"Helicoverpa gelotopoeon |Heliothis virescens|Neotuerta platensis|heliothines| sex pheromone|antagonist|attraction|inhibition|Lepidoptera|Noctuidae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5073,"idioma_id":4,"titulo":"Evaluation of oil-in-water emulsions with cationic ? anionic surfactants mixtures for potential use in the oil industry","resumen":"tThe emulsifier properties of sodium oleate (NaOl)-hexadecyltrimethylammonium bromide (HTAB) aque-ous mixtures were studied using different proportions of the surfactants. The formation of O\/W andW\/O emulsions was explored and their properties (viscosity, stability and droplets size distribution)were determined. The mixture with 0.75 mole fraction of HTAB without considering the solvent formedvery stable and concentrated O\/W emulsions, which were destroyed via heterocoagulation by quartzitesand. Thus, these mixtures have high potential applicability in the asphalt emulsification for pavementproduction or sand fixation.","palabras_clave":"Petroleum emulsions|Catanionic emulsifier|Sodium oleate|oleateHexadecyltrimethylammonium bromide|bromideMixed surfactantsa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5074,"idioma_id":4,"titulo":"Improving bone cement toughness and contrast agent confinement  by using acrylic branched polymers","resumen":"A new biomedical material to be used as part of acrylic bone cement formulations is described. This new material is tough, its Young's Modulus is similar to the one of poly (methylmethacrylate) and the contrast agent, usually employed in acrylic bone cements, is homogeneously distributed among the polymeric matrix. Additionally, its wear coefficient is 66% lower than the one measured in poly(methyl methacrylate). The developed material is a branched polymer with polyisoprene backbone and poly(methyl methacrylate) side chains, which are capable of retaining barium sulphate nanoparticles thus avoiding their aggregation. The grafting reaction was carried out in presence of the nanoparticles, using methyl methacrylate as solvent. From the 1H-NMR spectra it was possible to determine the average number of MMA units per unit of isoprene (3.75:1). The ability to retain nanoparticles (about 8 wt.%), attributed to their interaction with the polymer branches, was determined by thermogravimetric analysis and confirmed by FTIR and microscopy techniques. By SEM microscopy it was also possible to determine the homogeneous spatial distribution of the barium sulphate nanoparticles along the polymer matrix.","palabras_clave":"Branched polymer|Poly(methyl methacrylate)|Natural rubber|Polyisoprene|Barium sulphate nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5075,"idioma_id":4,"titulo":"The phacopid trilobite Echidnops taphomimus n. sp. from the Lower Devonian of Argentina: insights into infaunal molting, eye structure and geographic distribution","resumen":"Some Silurian-Devonian Argentinian trilobites characterized by infaunal behavior during molting are considered. After a taxonomic reappraisal, a species previously referred to a lineage of the phacopid Paciphacops is proposed as Echidnops taphomimus new species, from the Lower Devonian (probably late Lochkovian) of the Talacasto Formation, Argentine Precordillera. The visual surface of E. taphomimus indicates that a nearly irregular pattern of lens arrangement, typical of early phacopids such as the Ordovician Ormathops, can also be recognized in more derived Devonian relatives, providing new insights on some evolutionary aspects of visual development. Echidnops is recognized in Australia and Argentina, recording an unusual distribution pattern in trilobites from the Lower Devonian of southern South America, otherwise mostly linked to faunas of related Gondwanan, austral circum-polar, Devonian basins of the Malvinokaffric Realm. In accordance with proposed increasing predation pressure of durofagous predators in the context of the Mid Paleozoic Marine Revolution, evidence indicates that the infaunal molting behavior, as defensive strategy, arose in several trilobite groups during Silurian-Devonian times, rather than in a single endemic lineage of Paciphacops species.","palabras_clave":"TRILOBITA|DEVONIAN|MOULTING|SCHIZOCHROAL EYE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5076,"idioma_id":4,"titulo":"Enhanced spectrophotometric detection of Hg in water samples by surface plasmon resonance of Au nanoparticles after preconcentration with vortex-assisted liquid-liquid microextraction","resumen":"This article presents an efficient, simple, and cost-effective method for the determination of trace amounts of Hg by vortex-assisted liquid-liquid microextraction (VALLME) coupled to microvolume UV?Vis spectrophotometry. This method correlates changes in the intensity of localized surface plasmon resonance (LSPR) of tetraoctylammonium bromide (TOABr) coated Au nanoparticles (NPs) after interaction with Hg2+ ion. Spectroscopic measurements of the TOABr-coated Au NPs phase with particular absorption properties (strong andwelldefined absorption bands) after analyte extraction by VALLME, provide an accurate and sensitive determination of Hg in water samples, comparablewith measurements obtained by atomic absorption spectrometry (AAS). Different variables including sample volume, extraction time, and TOABr-coated Au NPs dispersion volume were carefully studied; final experimental conditions were 5 mL, 120 &#956;L and 5 min respectively. The limit of detection (LOD) was 0.8 ng mL&#8722;1. The calibration curve was linear at concentrations between the limit of quantification (LOQ) (4.9 ng mL&#8722;1) and up to at least 120 ng mL&#8722;1 of Hg. The relative standard deviation for six replicate determinations of 20 ng mL&#8722;1 of Hg was 4.7%. This method exhibited an excellent analytical performance in terms of selectivity and sensitivity and it was finally applied for Hg determination in spiked tap and mineral water samples.","palabras_clave":"nanoparticles|microextraction|surface plasmon resonance|mercury"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5077,"idioma_id":4,"titulo":"Internal seed dispersal by parrots: an overview of a neglected mutualism","resumen":"Despite the fact that parrots (Psitacifformes) are generalist apex frugivores, they have largely been considered plant antagonists and thus neglected as seed dispersers of their food plants. Internal dispersal was investigated by searching for seeds in faeces opportunistically collected at communal roosts, foraging sites and nests of eleven parrot species in different habitats and biomes in the Neotropics. Multiple intact seeds of seven plant species of five families were found in a variable proportion of faeces from four parrot species. The mean number of seeds of each plant species per dropping ranged between one and about sixty, with a maximum of almost five hundred seeds from the cacti Pilosocereus pachycladus in a single dropping of Lear?s Macaw (Anodorhynchus leari). All seeds retrieved were small (","palabras_clave":"SEED DISPERSAL|ENDOZOO|MUTUALISM|ENDOZOOCHORY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5078,"idioma_id":4,"titulo":"Large-scale impacts of multiple co-occurring invaders on monkey puzzle forest regeneration, native seed predators and their ecological interactions","resumen":"Most ecosystems of the world are being increasingly invaded by a variety of alien species. However, little is known about the combined ecological impacts of multiple co-occurring invaders. We assessed the impact of a community of exotic mammals (five domestic and four wild) on forests of monkey puzzle (Araucaria araucana), a globally endangered tree restricted to ca 400 km on the slopes of the Andes in Chile and Argentina. Seeds of monkey puzzles provide food during winter to a small community of native mice and Austral parakeets (Enicognathus ferrugineus). We recorded the number of uneaten seeds and the number of young seedlings at the end of winter under 516 female monkey puzzle trees located across the species? distribution, and identified the signals of native and exotic species that visited the under-canopy of each tree. Moreover, we studied the diet and foraging behavior of Austral parakeets to explore the potential indirect effects of exotic mammals through the disruption of a key ecosystem service (seed dispersal) supposedly provided by parakeets. All but one tree were visited by at least one seed predator species. Austral parakeets and mice predated seeds from 85% and at least 45% of the trees, respectively, and both the number of remaining seeds and seedlings were significantly larger when only parakeets or mice predated seeds than when exotic mammals also visited the trees. At least 90% of trees were visited by one or more exotic species, and the number of seeds and seedlings dropped drastically when at least two and four exotic species visited the tree, respectively. Austral parakeets mostly foraged on monkey puzzle trees during the winter period and dispersed their seeds in most feeding instances once seeds fell to the ground. The proliferation of exotic mammals may reduce the populations of native seed-predators in the long-term as well as the regeneration of monkey puzzle forests, directly through a reduction of seed availability and seedling survival, and indirectly through the disruption of dispersal processes. Our results show how strategies based on protecting areas may assure the survival of individuals of this long-lived (up to 1300 yr) tree species. However, forest regeneration and the community of native seed-predators and related ecological processes further depend on the proper management of exotic mammal invasions.","palabras_clave":"Biological invasions|Ecological feed-backs|Ecological impacts; |Mutualism interactions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5079,"idioma_id":4,"titulo":"Movement decisions in natural catastrophes: how a flying scavenger deals with a volcanic eruption","resumen":"Understanding how organisms react to natural catastrophes is of special interest for ecologists and managers since they may drive changes in species distributions and abundance, and indeed cause extinctions. Particularly, explosive volcanic eruptions promote major changes in the environment forcing organisms to make decisions, first allowing them survive and then, to obtain the best balance between the costs and benefits derived from such changes. Our objective was to study the movement decisions of a large scavenger bird, the Andean condor (Vultur gryphus), facing a volcanic eruption that injected millions of tons of abrasive ash into the atmosphere causing pronounced changes in the environments where these birds reside. By using a large dataset of GPS-based locations obtained before, during and after the eruption, we explored how the ash fall and subsequent changes in livestock mortality patterns affected the movement behaviour of the studied species. Interestingly, we found that the birds: (1) did not show significant changes in their home ranges; (2) reacted to the ash plume only a very short distances from it and; (3) did not change their foraging habitat preferences in accordance with the patterns of livestock mortality caused by the eruption. Our work discusses the various trade-offs that free-ranging animals perceive under conditions of natural catastrophe and how movement decisions may eventually alter fitness-related traits","palabras_clave":"Andean condor|Movement behavior|Volcanic ash|Vultur gryphus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5080,"idioma_id":4,"titulo":"Overexpression of bromodomain factor 3 in Trypanosoma cruzi (Tc BDF3) affects differentiation of the parasite and protects it against bromodomain inhibitors","resumen":"The bromodomain is the only protein domain known to bind acetylated lysine. In the last few years many bromodomain inhibitors have been developed in order to treat diseases such as cancer caused by aberrant acetylation of lysine residues. We have previously characterized Trypanosoma cruzi bromodomain factor 3 (TcBDF3), a bromodomain with an atypical localization that binds acetylated &#945;-tubulin. In the present work we show that parasites overexpressing TcBDF3 exhibit altered differentiation patterns and are less susceptible to treatment with bromodomain inhibitors. We also demonstrate that recombinant TcBDF3 is able to bind to these inhibitors in vitro in a concentration-dependant manner. In parallel, the overexpression of a mutated version of TcBDF3 negatively affects growth of epimastigotes. Recent results, including the ones presented here, suggest that bromodomain inhibitors can be conceived as a new type of anti-parasitic drug against trypanosomiasis.","palabras_clave":"Enfermedad de Chagas|Bromodominios|citoesqueleto"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5082,"idioma_id":4,"titulo":"Potential problems of removing one invasive species at a time: a meta-analysis of the interactions between invasive vertebrates and unexpected effects of removal programs","resumen":"Although the co-occurrence of nonnative vertebrates is a ubiquitous global phenomenon, the study of interactions between invaders is poorly represented in the literature. Limited understanding of the interactions between co-occurring vertebrates can be problematic for predicting how the removal of only one invasive?a common management scenario?will affect native communities. We suggest a trophic food web framework for predicting the effects of single-species management on native biodiversity. We used a literature search and meta-analysis to assess current understanding of how the removal of one invasive vertebrate affects native biodiversity relative to when two invasives are present. The majority of studies focused on the removal of carnivores, mainly within aquatic systems, which highlights a critical knowledge gap in our understanding of co-occurring invasive vertebrates. We found that removal of one invasive vertebrate caused a significant negative effect on native species compared to when two invasive vertebrates were present. These unexpected results could arise because of the positioning and hierarchy of the co-occurring invasives in the food web (e.g., carnivore?carnivore or carnivore?herbivore). We consider that there are important knowledge gaps to determinate the effects of multiple co-existing invaders on native ecosystems, and this information could be precious for management.","palabras_clave":"animal behavior|biodiversity|conservation biology|ecology|zoology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5083,"idioma_id":4,"titulo":"Transcriptome profiling of Diachasmimorpha longicaudata towards useful molecular tools for population management","resumen":"Background: Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a solitary parasitoid of Tephritidae(Diptera) fruit flies of economic importance currently being mass-reared in bio-factories and successfully usedworldwide. A peculiar biological aspect of Hymenoptera is its haplo-diploid life cycle, where females (diploid)develop from fertilized eggs and males (haploid) from unfertilized eggs. Diploid males were described in manyspecies and recently evidenced in D. longicaudata by mean of inbreeding studies. Sex determination in thisparasitoid is based on the Complementary Sex Determination (CSD) system, with alleles from at least one locusinvolved in early steps of this pathway. Since limited information is available about genetics of this parasitoidspecies, a deeper analysis on D. longicaudata?s genomics is required to provide molecular tools for achieving amore cost effective production under artificial rearing conditions.Results: We report here the first transcriptome analysis of male-larvae, adult females and adult males of D.longicaudata using 454-pyrosequencing. A total of 469766 reads were analyzed and 8483 high-quality isotigs wereassembled. After functional annotation, a total of 51686 unigenes were produced, from which, 7021 isotigs and20227 singletons had at least one BLAST hit against the NCBI non-redundant protein database. A preliminarycomparison of adult female and male evidenced that 98 transcripts showed differential expression profiles, with atleast a 10-fold difference. Among the functionally annotated transcripts we detected four sequences potentiallyinvolved in sex determination and three homologues to two known genes involved in the sex determinationcascade. Finally, a total of 4674SimpleSequence Repeats (SSRs) were in silico identified and characterized.Conclusion: The information obtained here will significantly contribute to the development of D. longicaudatafunctional genomics, genetics and population-based genome studies. Thousands of new microsatellite markerswere identified as toolkits for population genetics analysis. The transcriptome characterized here is the startingpoint to elucidate the molecular bases of the sex determination mechanism in this species.","palabras_clave":"Transcriptome|Genomics|Diachasmimorpha longicaudata|Sex determination"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5084,"idioma_id":4,"titulo":"Reproductive strategy of the copepod Acartia tonsa in a hypersaline estuary of Southwestern Atlantic. Seasonal morphology of eggs and ecological implications","resumen":"This study investigates the seasonal variation of the production, morphology and hatching success of Acartia tonsa eggs taking into account the variations of female?s size, population abundance and environmental factors in a turbid and hypersaline estuary. Sampling were performed considering the favorable (18-23°C and 32-36 salinity) and unfavorable periods (5-7ºC; 32-38) for this species in the Bahía Blanca Estuary (BBE). Incubation of field females were performed in laboratory simulating in situ condition and specimens from fixed samples were counted. Observations of egg?s morphology were made by SEM. A positive relationship of egg production with increasing temperature and salinity was revealed. An inverse relationship of the sizes of females and eggs with decreasing temperature was also detected. Acartia tonsa displayed a marked seasonality in its reproductive strategy which enabled it to the maintenance in the water column all year round in the BBE. To ensure high offspring during favorable period, this species invested its energy in the production of subitaneous eggs (12.95±2.38 eggs f-1d-1) with smooth appearance and high percentage of viable eggs. Otherwise, during unfavorable period, A. tonsa invests energy in body mass as well as in the production of three egg morphotypes (6.56±3.2 eggs f-1d-1) which respond to a phenotypic evolution to ensure its survival. Despite of differences in the length and shape of the egg s ´ spines, these three types of eggs had the same delayed hatching behavior and the eggs with shorter spines would integrate the resting egg bank in the bottom sediments of the estuary.","palabras_clave":"ACARTIA TONSA|SEASONAL FECUNDITY|REPRODUCTIVE PLASTICITY|EGGS MORPHOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5086,"idioma_id":4,"titulo":"Development of phytotoxicity indexes and their correlation with  ecotoxicological, stability and physicochemical parameters during  passive composting of poultry manure","resumen":"Both raw and composted poultry manure is applied as soil amendment. The aims of this study were: (1) to develop phytotoxicity indexes for organic wastes and composts, and (2) to assess the correlation among phytotoxicity indexes, ecotoxicological endpoints and stability and physicochemical parameters during passive composting of poultry manure. Six 2-m(3) composting piles were constructed and four parameter groups (physicochemical and microbiological parameters, ecotoxicological endpoints, and biological activity) were determined at four sampling times during 92days. Extracts were used to carry out acute toxicity tests on Daphnia magna, Lactuca sativa and Raphanus sativus. Composting decreased average toxicity 22.8% for the 3 species and D. magna was the most sensitive species. The static respiration index decreased from 1.12 to 0.46mgO2gOMh(-1) whilst organic matter reduced by 64.1% at the end of the process. Escherichia coli colonies remained higher than values recommended by international guidelines. The D. magna immobilization test allowed the assessment of possible leachate or run-off toxicity. The new phytotoxicity indexes (RGIC0.8 and GIC80%), proposed in this study, as well as salinity, proved to be good maturity indicators. Hence, these phytotoxicity indexes could be implemented in monitoring strategies as useful ecotoxicological tools. Multivariate analyses demonstrated positive correlations between ecotoxicological endpoints (low toxicity) and biological activity (stability). These two parameter groups were associated at the final sampling time and showed negative correlations with several physicochemical parameters (organic and inorganic contents). The final poultry manure compost was rendered stable, but immature and, thus, unsuitable for soil amending.","palabras_clave":"Daphnia magna|lactuca sativa|maturity|phytotoxicity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5087,"idioma_id":4,"titulo":"An oxidative burst and its attenuation by bacterial peroxidase activity is required for optimal  establishment of  the Arachis hypogaea-Bradyrhizobium sp symbiosis","resumen":"The main purpose of this study was to determine whether the Arachishypogaea L. root oxidative burst, produced at early stages of its symbiotic interaction with Bradyrhizobium sp. SEMIA 6144, and the bacterial antioxidant system are required for the successful development of this interaction.Methods and Results: Pharmacological approaches were used to reduce bothplant oxidative burst and bacterial peroxidase enzyme activity. In plants whose H2O2 levels were decreased, a low nodule number, a reduction in theproportion of red nodules (%) and an increase in the bacteroid density were found. The symbiotic phenotype of plants inoculated with a Bradyrhizobium sp. SEMIA 6144 culture showing decreased peroxidase activity was also affected, since the biomass production, nodule number and percentage of red nodules in these plants were lower than in plants inoculated with Bradyrhizobium sp. control cultures.Conclusions: We demonstrated for the first time that the oxidative bursttriggered at the early events of the symbiotic interaction in peanut, is a prerequisite for the efficient development of root nodules, and that the antioxidant system of bradyrhizobial peanut symbionts, particularly the activity of peroxidases, is counteracting this oxidative burst for the successful establishment of the symbiosis.Significance and Impact of the Study: Our results provide new insights into the mechanisms involved in the development of the symbiotic interaction established in A. hypogaea L. a legume infected in an intercellular way","palabras_clave":"ANTIOXIDANT SYSTEM|ARACHIS HYPOGAEA L.|BRADYRHIZOBIUM SP|HYDROGEN PEROXIDE|OXIDATIVE BURST|SYMBIOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5088,"idioma_id":4,"titulo":"Sea level anomaly on the Patagonian continental shelf: Trends, annual patterns and geostrophic flows","resumen":"We study the annual patterns and linear trend of satellite sea level anomaly (SLA) over the southwest South Atlantic continental shelf (SWACS) between 54oS and 36oS. Results show that south of 42°S the thermal steric effect explains nearly 100% of the annual amplitude of the SLA, while north of 42°S it explains less than 60%. This difference is due to the halosteric contribution. The annual wind variability plays a minor role over the whole continental shelf. The temporal linear trend in SLA ranges between 1 and 5 mm\/yr (95% confidence level). The largest linear trends are found north of 39°S, at 42°S and at 50°S. We propose that in the northern region the large positive linear trends are associated with local changes in the density field caused by advective effects in response to a southward displacement of the South Atlantic High. The causes of the relative large SLA trends in two southern coastal regions are discussed as a function meridional wind stress and river discharge. Finally, we combined the annual cycle of SLA with the mean dynamic topography to estimate the absolute geostrophic velocities. This approach provides the first comprehensive description of the seasonal component of SWACS circulation based on satellite observations. The general circulation of the SWACS is northeastward with stronger\/weaker geostrophic currents in austral summer\/winter. At all latitudes, geostrophic velocities are larger (up to 20 cm\/s) close to the shelf-break and decrease towards the coast. This spatio-temporal pattern is more intense north of 45°S.","palabras_clave":"SEA LEVEL |SEASONAL CYCLE|CURRENTS|TRENDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5089,"idioma_id":4,"titulo":"Malvinas Current variability from Argo floats and satellite altimetry","resumen":"The Malvinas Current (MC) is an offshoot of the Antarctic Circumpolar Current (ACC). Downstream of Drake Passage, the northern fronts of the ACC veer northward, cross over the North Scotia Ridge (NSR) and the Malvinas Plateau and enter the Argentine Basin. We investigate the variations of the MC circulation between the NSR and 41°S and their possible relations with the ACC circulation using data from Argo floats and satellite altimetry. The data depict meandering and eddy-shedding of the northern ACC jets as they cross the NSR. The altimetry fields show that these eddies are trapped, break down and dissipate over the Malvinas Plateau, suggesting that this region is a hot spot for dissipation of mesoscale variability. Variations of sea level anomalies (SLA) across the NSR do not impact the MC further north, except for intra-seasonal variability associated with coastal trapped waves. Altimetry and float trajectories show events during which a large fraction of the MC is cut off from the ACC. Blocking events at around 48.5°S are a recurrent feature of the MC circulation. Over the 23 year altimetry record, we detected 26 events during which the MC surface transport at 48.5°S was reduced to less than half its long term mean. Blocking events last from 10 to 35 days and do not present any significant trend. These events were tracked back to positive SLA that built up over the Argentine Abyssal Plain. Future work is needed to understand the processes responsible for these blocking events. This article is protected by copyright. All rights reserved.","palabras_clave":"satellite altimetry|argo|malvinas current"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5091,"idioma_id":4,"titulo":"Exploitation of faunal resources by hunter-gatherers in the center of the pampa grasslands during the Holocene. The archaeofauna of the Laguna Cabeza de Buey 2 site (San Carlos de Bolivar, Buenos Aires, Argentina)","resumen":"The purpose of this paper is to present a detailed zooarchaeological study conducted on the boneassemblage from the Laguna Cabeza de Buey 2 site, located on the margins of a small shallow lake in thecenter of the Pampa grasslands (province of Buenos Aires, Argentina). Taxonomic structure, taphonomicinformation, site formation processes, and subsistence strategies for different occupation events aredescribed and discussed. At least three events of pre-Hispanic occupation (two in the Middle Holocene[6800 and 4150 14C years BP] and one in the Late Holocene) and one in Historical moments wereidentified. Human occupations are developed in relation to a shallow lake under a climate more aridduring the Middle Holocene, which decreased up to the current environmental conditions; humidtemperate. The camelid Lama guanicoe (guanaco) was always the most important economic resourceduring the three events of human occupation in the Holocene, followed by Ozotoceros bezoarticus(pampean deer) and, to a lesser extent, by three species of small armadillos (Chaetophractus sp., Zaedyuspichiy, and Tolypeutes matacus). In Historical times, modern domestic faunal remains (Equus caballus andBos taurus) were exploited. A subsistence strategy focused on artiodactyls, mainly guanaco, allowssuggesting certain specialization through time in the exploitation of animal resources by hunter-gatherergroups in the center of the Pampa grasslands, strategy that contrasts with what is recorded in other areasof the Pampean region.","palabras_clave":"Hunter-gatherers|Zooarchaeology|Subsistence|Pampa grasslands|Middle\/Late Holocene"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5092,"idioma_id":4,"titulo":"TUMOR BIOLOGY OF NON-METASTATIC STAGES OF CLEAR CELL RENAL CELL CARCINOMA; OVER EXPRESSION OF STEAROYL DESATURASE-1, EPO\/EPO-R SYSTEM AND HYPOXIA-RELATED PROTEINS","resumen":"Clear cell renal cell carcinoma (ccRCC) is the most common subtype of renal carcinomas. There is great interest to know the molecular basis of the tumor biology of ccRCC that might contribute to a better understanding of the aggressive biological behavior of this cancer and to identify early biomarkers of disease. This study describes the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (hypoxia-inducible factor (HIF)-1&#945;, erythropoietin (EPO), vascular endothelial growth factor (VEGF)), their receptors (EPO-R, VEGFR-2), and stearoyl desaturase-1 (SCD-1) in early stages of ccRCC. Tissue samples were obtained at the Urology Unit of the J.R. Vidal Hospital (Corrientes, Argentina), from patients who underwent radical nephrectomy for renal cancer between 2011 and 2014. Four experimental groups according to pathological stage and nuclear grade were organized: T1G1 (n = 6), T2G1 (n = 4), T1G2 (n = 7), and T2G2 (n = 7). The expression of HIF-1&#945;, EPO, EPO-R, VEGF, VEGFR-2, Bcl-xL, and SCD-1 were evaluated by immunohistochemistry, Western blotting, and\/or RT-PCR. Apoptosis was assessed by the TUNEL in situ assay, and tumor proliferation was determined by Ki-67 immunohistochemistry. Data revealed that HIF-1&#945;, EPO, EPO-R, VEGF, and VEGF-R2 were overexpressed in most samples. The T1G1 group showed the highest EPO levels, approximately 200 % compared with distal renal tissue. Bcl-xL overexpression was concomitant with the enhancement of proliferative indexes. SCD-1 expression increased with the tumor size and nuclear grade. Moreover, the direct correlations observed between SCD-1\/HIF-1&#945; and SCD-1\/Ki-67 increments suggest a link among these molecules, which would determine tumor progression in early stages of ccRCC. Our results demonstrate the relationship among proliferation, survival, and apoptosis with the expression of key molecules related to tumoral hypoxia (HIF-1&#945;, EPO, VEGF), their receptors (EPO-R, VEGFR-2), and SCD-1 in early stages of ccRCC.","palabras_clave":"RENAL CARCINOMA|HYPOXIA INDUCIBLE FACTOR|ERYTHROPOIETIN|STEAROYL DESATURASE-1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5093,"idioma_id":4,"titulo":"Carbon and Nitrogen Sources Influence Tricalcium Phosphate Solubilization and Extracellular Phosphatase Activity by Talaromyces flavus","resumen":"The aim of this work was to study phosphate (P) solubilization (and the processes involved in this event) by Talaromyces flavus (BAFC 3125) as a function of carbon and\/or nitrogen sources. P solubilization was evaluatedin NBRIP media supplemented with different carbon (glucose, sorbitol, sucrose, and fructose) and nitrogen (L-asparagine, urea, ammonium sulfate (AS), and ammonium nitrate (AN) combinations. The highest P solubilization was related to the highest organic acid production (especially gluconic acid) and pH drop for those treatments where glucose was present. Also P solubilization was higher when an inorganic nitrogen source was supplemented to the media when compared to an organic one. Although not being present an organic P source, phosphataseactivity was observed. This shows that P mineralization and P solubilization can occur simultaneously, and that P mineralization is not induced by the enzymesubstrate. The combination that showed highest P solubilization was for AN-glucose. The highest acid phosphatase activity was for AS-fructose, while for alkaline phosphatase were for AS-fructose and AN-fructose. Acid phosphatase activity was higher than alkaline. P solubilization and phosphatase activity (acid and alkaline) were influenced by the different carbon?nitrogen combinations. A better understanding of phosphate-solubilizing fungi could bring a better use of soil P.","palabras_clave":"phosphate-solubilizing fungi|Talaromyces flavus|phosphatase|organic acids"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5094,"idioma_id":4,"titulo":"Synthesis and antifungal activity of bile acid-derived oxazoles","resumen":"Peracetylated bile acids (1a?g) were used as starting materials for the preparation of fourteen new derivatives bearing an oxazole moiety in their side chain (6a?g, 8a?g). The key step for the synthetic path was a Dakin?West reaction followed by a Robinson?Gabriel cyclodehydration. A simpler model oxazole (12) was also synthesized. The antifungal activity of the new compounds (6a?g) as well as their starting bile acids (1a?g) was tested against Candida albicans. Compounds 6e and 6g showed the highest percentages of inhibition (63.84% and 61.40% at 250 &#956;g\/mL respectively). Deacetylation of compounds 6a?g, led to compounds 8a?g which showed lower activities than the acetylated derivatives.","palabras_clave":"BILE ACIDS|CANDIDA ALBICANS|OXAZOLES|DAKIN-WEST REACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5095,"idioma_id":4,"titulo":"Fruit selection by Andean forest birds: influence of fruit functional traits and their temporal variation","resumen":"Fruit selection, i.e., the consumption of fruits disproportionately to their availability, results from the interaction between diet preferences and ecological factors that modify them. We assessed the importance of functional fruit traits to explain fruit selection by birds in Andean subtropical forests, taking into account temporal variation in trait distribution in the assembly of available fruits. During 2 yr, we measured the abundance of ripe fruits and their consumption by birds in a 6-ha plot during 11 bimonthly samplings, and we used 17 phenological, morphological, and nutritional traits to characterize fruits selected by four bird species. Fruit selection was pervasive year-round, highly variable over time and across bird species. Fruit species were selected over time periods shorter than their ripening phenology, and the selection of fruits with particular traits was specific to the fruit-eating species. Maximization in pulp reward per consumed fruit seems to be the main driving force behind fruit selection, indicating that birds select fruits with traits that directly affect net energy gain. Our results can be interpreted in a framework of a hierarchy of foraging decisions, under which the spatiotemporal context of the fruiting environment modifies the relative intake rates of a particular fruit, while the ability to discriminate fruit contents becomes increasingly important on a smaller dimension. We show that fruit-selection properties are contingent on specific fruit traits and particular spatiotemporal conditions, which modify the structure of mutualistic interactions.","palabras_clave":"ANDEAN FORESTS|FEEDING SELECTION|FRUIT SHAPE|FRUIT-EATING BIRDS|FUNCTIONAL TRAIT|NEOTROPICS|NUTRIENT CONTENT|PULP-TO-SEED RATIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5097,"idioma_id":4,"titulo":"Validity in a dialetheist framework","resumen":"In this paper, we develop two theories of validity in a dialetheist framework,  both based on Meadows (2014). The first one, LPV* , has LP´s consequence relation but the validity predicate of Meadows? fixed point construction. The second theory, DT (the one we favour), is defined in terms of its validity predicate. Therefore, in DT, the validity predicate and the consequence relation coincide. Moreover, this theory, unlike Meadows? VAL, is reflexive.","palabras_clave":"VALIDITY|PARADOX|DIALETHEISM|SELF-REFERENCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5098,"idioma_id":4,"titulo":"Protection and functionalization of AISI 316 L for orthopedic implants: hybrid coating and sol gel glasses by spray to promote bioactivity","resumen":"This work presents the synthesis and deposition of hybrid sol-gel coating applied on AISI 316Lstainless steel by dipping with the aim of providing corrosion protection to the substrate. On the top of this coating a potentially bioactive glass (58S) made also by sol gel method with tetraethoxysilane (TEOS), triethyl phosphate (TEP) and calcium nitrate as precursors, was applied by spray technique. This method allows the creation of a wide distribution of particles sizes on the target. Coatings were characterized by profilometry and optical and electronic microscopy. The surface integrity and corrosion resistance of the coatings in time is analyzed by electrochemical tests in simulated body fluid (SBF).It is observed some kind of detrimental effect regarding the corrosion behavior between the bioactive dots and the underneath coating. Nevertheless the entire system results promising for further investigation since the addition of 58S bioactive particles by spray method results appealing as it is a simple way to generate bioactive points on the implant surface.","palabras_clave":"SOL-GEL|COATINGS|BIOACTIVE GLASS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5099,"idioma_id":4,"titulo":"Whey permeate containing galacto-oligosaccharides as a medium for biomass production and spray drying of Lactobacillus plantarum CIDCA 83114","resumen":"Whey permeate (WP) is a low-cost waste product that can be used as a growth media of probiotic bacteria and as a source of galacto-oligosaccharides (GOS) being an excellent alternative to obtain probiotic biomass in a more economical way. The aim of this work was to evaluate the suitability of usingWP and WP enriched with GOS (WP-GOS) as a culture broth and as a carrier for probiotic Lactobacillus plantarum CIDCA 83114 to obtain viable dehydrated bacteria using spray drying. This strain was able togrow satisfactorily in unsupplemented WP showing a similar behavior in WP and WP-GOS. It alsoperformed well in spray drying. Viability of dehydrated lactobacilli was monitored throughout thestorage of powders at 20 C for 10 weeks. Survival during storage of L. plantarum grown and dehydrated in WP-GOS was significant higher than strain grown and dehydrated in WP at the end of storage time.Strain grown in WP increased their tolerance to acid conditions and the presence of GOS increased significantly its survival at low pH environment in dehydrated condition. L. plantarum CIDCA 83114 grown and dehydrated in WP-GOS constitute a low-cost spray-dried preparation containing high concentration of viable bacteria with enhanced gastrointestinal passage resistance..","palabras_clave":"Whey permeate  |Lactobacillus plantarum|Galacto-oligosaccharides|Spray drying"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5100,"idioma_id":4,"titulo":"Production in stirred-tank bioreactor of recombinant bovine chymosin B by a high-level expression transformant clone of 'Pichia pastoris'","resumen":"An intense screening of P. pastoris clones transformed with the gene of bovine chymosin under methanol-inducible AOX1 promoter was performed, obtaining a transformant clone with a higher milk-clotting activity value in comparison with our previous studies. The scaling of recombinant-chymosin production was carried out by a fed-batch strategy in a stirred-tank bioreactor using biodiesel-byproduct crude glycerol as the carbon source and pure methanol for the induction of chymosin expression, achieving a biomass concentration of 158 g DCW\/L and a maximum coagulant activity of 192 IMCU\/ml after 120 h of methanol induction. Recombinant bovine chymosin was purified from bioreactor-fermentation culture by a procedure including anion-exchange chromatography which allowed obtaining heterologous chymosin with high level of purity and activity; suggesting that this downstream step could be scaled up in a successful manner for chymosin purification. Thermoestability assay permitted to establish that unformulated recombinant chymosin could be stored at 5 °C without decrease of enzyme activity throughout at least 120 days. Finally, reiterative methanol-inductions of recombinant chymosin expression in bioreactor demonstrated that the reutilization of cell biomass overcame the low enzyme productivity usually reached by P. pastoris system.","palabras_clave":"Bovine chymosin|Pichia pastoris|Methanol-inducible AOX1 promoter|Biodiesel-derived crude glycerol|Fed-batch fermentation|Protein purification|Reiterative methanol-inductions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5101,"idioma_id":4,"titulo":"Adsorption and characterization of MCPA on DDTMA- and raw-montmorillonite: Surface sites involved","resumen":"The 4-chloro-2-methylphenoxy acid (MCPA) is an herbicide widely used in agriculture, which generates a great concern about contamination of surface water and serious consequences for human health and the environment. In this work, the adsorption of MCPA on an Argentine montmorillonite (MMT) and its organo-montmorillonite product (OMMT) with different dodecyl trimethyl ammonium loading was investigated. MCPA adsorption on OMMT increases at least 3 times, with respect to the amount determined for MMT. X-ray diffraction and zeta potential analyses indicated the inner (interlayer) and outer surface participate as adsorption sites. Changes in surface electric charge and also interlayer expansion suggest that dimethyl amine (MCPA counterion) was also surface-adsorbed. The larger aggregates of OMMT, without and with MCPA, obtained compared to those of MMT samples, generate an improvement in the coagulation efficiency. This property, particularly after MCPA retention, allows an easier separation of the solids from the solution and enables a simple technological process application.","palabras_clave":"Adsorption|MCPA|characterization|organo-montmorillonite"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5102,"idioma_id":4,"titulo":"Alterations in triglyceride rich lipoproteins are related to endothelial dysfunction in metabolic syndrome","resumen":"Our aim was to analyze the effect of circulating triglyceride rich lipoprotein (TRL) on endothelial function in metabolic syndrome (MetS).METHODS: We studied 40 patients with MetS (ATPIII), divided into those presenting normal endothelial function (n=19) and those with endothelial dysfunction (n=21) by means of the evaluation of pulse wave velocity, before and after brachial artery ischemia. In fasting serum we measured lipid and lipoprotein profile, insulin and glucose (HOMA-IR). Moreover, isolated TRL (d<1006g\/l) were chemically characterized. In parallel, using randomly selected TRL from MetS patients with endothelial dysfunction (n=6) and MetS patients with normal endothelial function (n=6), the ability of TRL to inhibit ACh-induced vasorelaxation (10-9-10-5mM) on aortic rings previously pre-contracted by noradrenaline (10-8mM) was evaluated.RESULTS: Interestingly, TRL isolated from MetS patients presenting endothelial dysfunction showed triglyceride over-enrichment (59.1±4.8 vs. 54.1±4.7%; p=0.04), even after adjusting by potential confounders (p=0.05). In addition, while TRL resulting from both MetS groups significantly inhibited endothelium dependent vasorelaxation (p<0.001), TRL from MetS patients with endothelial dysfunction showed a strong tendency to a greater inhibition of vasorelaxation (p=0.06). Moreover, TRL-triglyceride (%) showed a strong tendency to correlate with the grade of vasorelaxation inhibition exerted by TRL (r=0.60; p=0.05).CONCLUSION: These results, taken together, would allow inferring for the first time that the predominance of triglyceride over-enriched TRL in circulation in MetS would induce endothelial dysfunction, contributing to the inherent cardiovascular risk of MetS.","palabras_clave":"Endothelial dysfunction|Endothelium|Metabolic syndrome|Triglyceride rich lipoprotein"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5103,"idioma_id":4,"titulo":"Role of protein phosphorylation and tyrosine phosphatases in the adrenal regulation of steroid synthesis and mitochondrial function","resumen":"In adrenocortical cells, adrenocorticotropin (ACTH) promotes the activation of several protein kinases. The action of these kinases is linked to steroid production, mainly through steroidogenic acute regulatory protein (StAR), whose expression and activity are dependent on protein phosphorylation events at genomic and non-genomic levels. Hormone-dependent mitochondrial dynamics and cell proliferation are functions also associated with protein kinases. On the other hand, protein tyrosine dephosphorylation is an additional component of the ACTH signaling pathway, which involves the 'classical' protein tyrosine phosphatases (PTPs), such as Src homology domain (SH) 2-containing PTP (SHP2c), and members of the MAP kinase phosphatase (MKP) family, such as MKP-1. PTPs are rapidly activated by posttranslational mechanisms and participate in hormone-stimulated steroid production. In this process, the SHP2 tyrosine phosphatase plays a crucial role in a mechanism that includes an acyl-CoA synthetase-4 (Acsl4), arachidonic acid (AA) release and StAR induction. In contrast, MKPs in steroidogenic cells have a role in the turn-off of the hormonal signal in ERK-dependent processes such as steroid synthesis and, perhaps, cell proliferation. This review analyzes the participation of these tyrosine phosphates in the ACTH signaling pathway and the action of kinases and phosphatases in the regulation of mitochondrial dynamics and steroid production. In addition, the participation of kinases and phosphatases in the signal cascade triggered by different stimuli in other steroidogenic tissues is also compared to adrenocortical cell\/ACTH and discussed.","palabras_clave":"PKA|PTPs|ERK1\/2|SHP2|MITOCHONDRIAL DYNAMICS|MKP-1|ACSL4"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5104,"idioma_id":5,"titulo":"Uso de atmósfera controlada y 1-MCP en kiwis ´Hayward´ producidos en Argentina: efectos sobre la maduración","resumen":"Seestudió el efecto del uso de la atmósfera controlada (AC), 1-metilciclopropeno(1-MCP) y su combinación, sobre la maduración del kiwi ?Hayward?. Frutoscosechados con 16,6% de materia seca y 9% de sólidos solubles totales (SST)fueron enfriados y tratados con 1000 ppb de 1-MCP (SmartFresh) durante 24h opermanecieron sin tratar con 1-MCP (controles). Posteriormente, frutascontroles y tratadas con 1-MCP fueron almacenadas en cámara de fríoconvencional (FC, 0°C y 90%HR) o en cámara de atmósfera controlada (AC, 2%O2y 5%CO2, 0°C; 90%HR) durante 5 meses (5m). Posteriormente, muestrasde todas las combinaciones de tratamiento permanecieron durante un mesadicional en FC (5m + 1m). A la cosecha, a los 5m y 5m + 1m y después de laposcámara (7 días a 20°C) se midieron el índice de color (en base a L*, a*, b*de CIE) y la firmeza de la pulpa (N), el SST (%) y la acidez titulable (AT, %).También se evaluó la dureza de columela al final de la poscámara, medianteanálisis sensorial. Los tratamientos afectaron fundamentalmente la firmeza dela pulpa y de la columela. Los frutos almacenados en AC resultaronsignificativamente más firmes que los almacenados en FC, tanto a los 5m como alos 5m + 1m, evidenciando el efecto residual de dicha tecnología. Igualresultado se encontró para dureza de columela. El uso de 1-MCP en combinacióncon la AC también mostró un efecto sinérgico sobre la firmeza a los 5m + 1m,permitiendo prolongar el periodo de almacenamiento de los frutos.","palabras_clave":"Actinidia deliciosa|ablandamiento|maduración"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5106,"idioma_id":4,"titulo":"Assessment of the root system of Brassica juncea (L.) czern. and Bidens pilosa L. exposed to lead polluted soils using rhizobox systems","resumen":"The purpose of this study was to compare the behaviorof the root system of one of the most frequently cited species inphytoremediation Indian mustard [Brassicajuncea (L.) Czern.] and a representative perennial herb (Bidens pilosa L.) which is a nativespecies in Argentina, and globally distributed, for different concentrations oflead in soils through chemical and visualization techniques of the rhizosphere.Lead polluted soils (from 1.85 to 981 µg g-1 Pb) from the vicinityof a lead recycling plant in the locality of Bouwer, were used in juxtaposedrhizobox systems planted with seedlings of B.juncea and B. pilosa with homogeneousand heterogeneous soil treatments. Root development,pH changes in the rhizosphere, dry weight of roots and aerial parts, leadcontent of root and aerial parts and potential extraction of lead byrhizosphere exudates were determined. In both species leadwas mainly accumulated in roots. However, although B. juncea accumulated significantly more lead than B. pilosa at elevated concentrations insoils, the latter achieved greater root and aerial development. No changes inthe pH of the rhizosphere associated to lead were observed, despite differentextractive potentials of lead in the exudates of the species analyzed beingobserved. Our results indicated that Indian mustard did not behave as ahyperaccumulator. ","palabras_clave":"RHIZOSPHERE|PB|PHYTOREMEDIATION|RHIZOBOX|BRASSICA JUNCEA|BRASSICA PILOSA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5107,"idioma_id":5,"titulo":"Eficiencia de uso de los recursos, productividad y producción en maíz según diferentes cultivos antecesores","resumen":"La producción de materia seca y grano depende de la habilidad de los cultivos en capturar los recursos. La intensificación sustentable involucra un incremento en la productividad del sistema a través de un uso más intenso de los mismos. Este trabajo pretendió aportar resultados regionales en cuanto a alternativas de secuencias agrícolas sobre eficiencia de uso de los recursos y productividad y producción del maíz. El ensayo se realizó durante 2014 y 2015 en Esperanza (Santa Fe). Se implantaron tres cultivos invernales y luego maíz de segunda con dos niveles de fertilización nitrogenada. La fertilización nitrogenada mejoró la materia seca total, el rendimiento y la eficiencia de uso de agua; pero, provocó una caída en la eficiencia de uso de N. La falta de respuesta del maíz con respecto a los cultivos antecesores fue atribuida a las características favorables del año y a la escasa productividad de la vicia.","palabras_clave":"ZEA MAYS L.|EFICIENCIA DE USO DE NITRÓGENO|EFICIENCIA DE USO DE AGUA|RENDIMIENTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5108,"idioma_id":4,"titulo":"The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity","resumen":"TaNAM transcription factors play an important role in controlling senescence, which in turn, influences the delivery of nitrogen, iron and other elements to the grain of wheat (Triticum aestivum) plants, thus contributing to grain nutritional value. While lack or diminished expression of TaNAMs determines a stay-green phenotype, the precise effect of these factors on chloroplast structure has not been studied. In this work we focused on the events undergone by chloroplasts in two wheat lines having either control or diminished TaNAM expression due to RNA interference (RNAi). It was found that in RNAi plants maintenance of chlorophyll levels and maximal photochemical efficiency of photosystem II were associated with lack of chloroplast dismantling. Flow cytometer studies and electron microscope analysis showed that RNAi plants conserved organelle ultrastructure and complexity. It was also found that senescence in control plants was accompanied by a low leaf enzymatic antioxidant activity. Lack of chloroplast dismantling in RNAi plants was associated with maintenance of protein and iron concentration in the flag leaf, the opposite being observed in control plants. These data provide a structural basis for the observation that down regulation of TaNAMs confers a functional stay-green phenotype and indicate that the low export of iron and nitrogen from the flag leaf of these plants is concomitant, within the developmental window studied, with lack of chloroplast degradation and high enzymatic antioxidant activity.","palabras_clave":"CHLOROPLAST|WHEAT|NAM|STAY GREEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5109,"idioma_id":4,"titulo":"Juvenile exposure to a high fat diet promotes behavioral and limbic alterations in the absence of obesity","resumen":"The incidence of metabolic disorders including obesity, type 2 diabetes and metabolic syndrome haveseriously increased in the last decades. These diseases ? with growing impact in modern societies ?constitute major risk factors for neurodegenerative disorders such as Alzheimer?s disease (AD), sharinginsulin resistance, inflammation and associated cognitive impairment. However, cerebral cellular andmolecular pathways involved are not yet clearly understood. Thus, our aim was to study the impact ofa non-severe high fat diet (HFD) that resembles western-like alimentary habits, particularly involvingjuvenile stages where the brain physiology and connectivity are in plain maturation. To this end, onemonth-oldC57BL\/6J male mice were given either a control diet or HFD during 4 months. Exposure to HFDproduced metabolic alterations along with changes in behavioral and central parameters, in the absenceof obesity. Two-month-old HFD mice showed increased glycemia and plasmatic IL1 but these valuesnormalized at the end of the HFD protocol at 5 months of age, probably representing an acute responsethat is compensated at later stages. After four months of HFD exposure, mice presented dyslipidemia,increased Lipoprotein-associated phospholipase A2 (Lp-PLA2) activity, hepatic insulin resistance andinflammation. Alterations in the behavioral profile of the HFD group were shown by the impediment innest building behavior, deficiencies in short and mid-term spatial memories, anxious and depressivelikebehavior. Regarding the latter disruptions in emotional processing, we found an increased neuralactivity in the amygdala, shown by a greater number of c-Fos+ nuclei. We found that hippocampal adultneurogenesis was decreased in HFD mice, showing diminished cell proliferation measured as Ki67+ cellsand neuronal differentiation in SGZ by doublecortin labeling. These phenomena were accompanied by aneuroinflammatory and insulin-resistant state in the hippocampus, depicted by a reactive phenotype inIba1+ microglia cells (increased in number and soma size) and an impaired response to insulin given bydecreased phosphorylated Akt levels and increased levels of inhibitory phosphorylation of IRS1. Our dataportray a set of alterations in behavioral and neural parameters as a consequence of an early-life exposureto a quite moderate high fat diet, many of which can resemble AD-related features. These results highlyemphasize the need to study how metabolic and neurodegenerative disorders are interrelated in deep,thus allowing the finding of successful preventive and therapeutic approaches.","palabras_clave":"Diet|Hippocampus|Cognition|Insulin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5110,"idioma_id":4,"titulo":"Computational chemistry to the rescue: modern toolboxes for the assignment of complex molecules by GIAO NMR calculations","resumen":"The calculation of NMR properties of molecules usingquantum chemical methods have deeply impacted severalbranches of organic chemistry. They are particularly important instructural or stereochemical assignments of organic compounds,with implications in total synthesis, stereoselective reactions andnatural products chemistry. In studying the evolution of thestrategies developed to support (or reject) a structural proposal,it comes clear that the most effective and accurate ones involvesophisticated procedures to correlate experimental andcomputational data. Owing to their relatively high mathematicalcomplexity, such calculations (CP3, DP4, ANN-PRA) are oftencarried out using additional computational resources provided bythe authors (such as applets or Excel files). This minireview willcover the state of the art of these toolboxes in the assignment oforganic molecules, including mathematical definitions, updatesand discussion of relevant examples.","palabras_clave":"NMR|GIAO|DFT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5111,"idioma_id":4,"titulo":"The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase","resumen":"Hypoxanthine phosphoribosyl transferase from Trypanosoma cruzi (TcHPRT) is a critical enzyme for the survival of the parasite. This work demonstrates that the full-length form in solution adopts a stable and enzymatically active tetrameric form, exhibiting large inter-subunit surfaces. Although this protein irreversibly aggregates during unfolding, oligomerization is reversible and can be modulated by low concentrations of urea. When the C-terminal region, which is predicted as a disordered stretch, is excised by proteolysis, TcHPRT adopts a dimeric state, suggesting that the C-terminal region acts as a main guide for the quaternary arrangement. These results are in agreement with X-ray crystallographic data presented in this work. On the other hand, the C-terminal region exhibits a modulatory role on the enzyme, as attested by the enhanced activity observed for the dimeric form. Bisphosphonates act as substrate-mimetics, uncovering long-range communications among the active sites. All in all, this work contributes to establish new ways applicable to the design of novel inhibitors that could eventually result in new drugs against parasitic diseases.","palabras_clave":"Quaternary structure| Enzymatic activity modulation|Stability|Proteolysis|Reversible oligomerization|Disorder C-terminal region|Bisphosphonates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5112,"idioma_id":4,"titulo":"Formation of volatile compounds, peptidolysis and carbohydrate fermentation by mesophilic lactobacilli and streptoccocci cultures in a cheese extract","resumen":"Many studies about the influence of Lactococcus lactis on cheese flavour have been reported, while the impact of mesophilic lactobacilli and Streptococcus thermophilus, either as single or mixed cultures, have been less studied. The ability of Lactobacillus paracasei 90, Lb. casei 72 (INLAIN collection) and S. thermophilus 2 (commercial strain) to produce flavour-related biochemical changes, as single or mixed (lactobacilli + streptococci) cultures, was assessed in a cheese model. These three strains, with different activities of glutamate dehydrogenase (GDH) and aminotransferases (AT), were incubated (14d\/37 °C) alone or in mixed cultures in a cheese model consisting of a sterile extract of a fresh cheese. Mesophilic lactobacilli showed fermentation of carbohydrates, an increase of peptidolysis and production of volatile compounds that were correlated with their AT activities. In addition, these strains also produced completely different profiles of the amino acceptor compounds: pyruvate and &#945;-ketoglutarate. S. thermophilus 2 increased the level of &#945;-ketoglutarate due to its high GDH activity, but its contribution to flavour compound production was negligible; small additional changes existed when S. thermophilus 2 was mixed with the lactobacilli. Biochemical changes leading to flavour formation were mainly due to the activity of lactobacilli. Our results suggest that the AT profile and the peptidolytic activity of each strain influenced the volatilome of the extracts during incubation. So, both lactobacilli strains could be used as adjunct cultures in cheeses to increase\/diversify the flavour, but more studies are needed to deepen the knowledge about the potential of S. thermophilus 2 for the production of flavour compounds.","palabras_clave":"STREPTOCOCCI|MESOPHILIC LACTOBACILLI|VOLATILE COMPOUNDS|CHEESE EXTRACT|CHEESE FLAVOUR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5113,"idioma_id":4,"titulo":"Influence of lactose hydrolysis on galacto -oligosaccharides, lactose, volatile profile and physicochemical parameters of different yogurt varieties","resumen":"BACKGROUND: Different types of reduced-lactose yogurt, obtained by lactose hydrolysis using &#946;-galactosidase enzyme, are commercially available. The breakdown of lactose modifies the carbohydrate profile, including the production of prebiotic galacto-oligosaccharides (GOS), which could affect the survival and activity of starter and probiotic cultures and the parameters of yogurt quality. The extension of thesechanges is dependent on the yogurt matrix composition. This study aimed to evaluate the influence of lactose hydrolysis on GOS, lactose, volatile profile and physicochemical parameters of different yogurt varieties during storage.RESULTS: The presence of &#946;-galactosidase enzyme did not affect neither the global composition nor the survival of cultures. Overall, the hydrolyzed products had lower acidity than traditional ones. GOS were found at similar levels in fresh hydrolyzed yogurts, whereas in traditional yogurts were not detected. The proportion of ketones,acids and aldehydes seems to be more dependent on yogurt variety than the addition of the enzyme. Likewise, the storage period affected the volatile fraction in different degree; the increase in acid compounds was more pronounced in hydrolyzed than traditional yogurts.CONCLUSION: This work shows that it is possible to obtain different varieties of reduced-lactose yogurt, some of them with additional benefits to health such as reduced-fat, reduced-calories, added with probiotic\/inulin and enriched in GOS, with similar characteristics to traditional products.","palabras_clave":"HYDROLYZED-LACTOSE YOGURTS|GALACTO-OLIGOSACCHARIDES|COMPOSITIONAL PARAMETERS|VOLATILE COMPOUND PROFILE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5114,"idioma_id":4,"titulo":"Swimming performance of Bradyrhizobium diazoefficiens is an emergent property of its two flagellar systems","resumen":"Many bacterial species use flagella for self-propulsion in aqueous media. In the soil, which is a complex and structured environment, water is found in microscopic channels where viscosity and water potential depend on the composition of the soil solution and the degree of soil water saturation. Therefore, the motility of soil bacteria might have special requirements. An important soil bacterial genus is Bradyrhizobium, with species that possess one flagellar system and others with two different flagellar systems. Among the latter is B. diazoefficiens, which may express its subpolar and lateral flagella simultaneously in liquid medium, although its swimming behaviour was not described yet. These two flagellar systems were observed here as functionally integrated in a swimming performance that emerged as an epistatic interaction between those appendages. In addition, each flagellum seemed engaged in a particular task that might be required for swimming oriented toward chemoattractants near the soil inner surfaces at viscosities that may occur after the loss of soil gravitational water. Because the possession of two flagellar systems is not general in Bradyrhizobium or in related genera that coexist in the same environment, there may be an adaptive tradeoff betweenenergetic costs and ecological benefits among these different species.","palabras_clave":"Bradyrhizobium|diazoefficiens|maldi tof|mass spectrometry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5115,"idioma_id":4,"titulo":"New kinetic insight into the spontaneous oxidation process of lithium in air by EPMA","resumen":"The exposure of metallic lithium to ambient air produces a fast corrosion reaction of the surface that continues to the bulk of the material. This spontaneous process was explored by means of X-ray Photoelectron Spectroscopy (XPS) and Electron Probe Micro-Analysis (EPMA). The combination of these techniques and Monte Carlo simulations allowed observing the formation of a film of lithium hydroxide (LiOH) and studying the growth within the material during the first 300 seconds. The corrosion is mainly due to the reaction of lithium with water and the diffusion of water through the growing LiOH film is the rate determining step. On the basis of a kinetic analysis we studied the mechanism of reaction and inferred a diffusion coefficient of D = (5.1±0.4)×10-12 cm2\/s in agreement with results from the literature obtained using different techniques.","palabras_clave":"Lithium corrosion|Electron Probe Micro-Analysis|Oxide growth kinetics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5116,"idioma_id":4,"titulo":"ASSESSING GROUNDWATER AGE IN CONFINED AQUIFERS FROM THE CENTRAL PAMPEAN PLAIN OF CORDOBA, ARGENTINA","resumen":"In the central-South region of Córdoba province, Argentina, people use mainly groundwater from confined aquifers systems (CAS) for different activities (agricultural, livestock farming, urban). Therefore it is necessary to conduct comprehensive studies for the planning of a more sustainable use considering groundwater renewal times can be of several thousands of years in the different aquifer systems. The objective of this research is to estimate groundwater age based on hydraulic, geochemical and isotopical methods. The CAS present variable extension, are multilayered and formed by thin (4-6m) sand-pebble lenses (K&#61566;1 m\/d to 30 m\/d). They are all linked to Neogene fluvial palaeosystems. These layers are situated at different depths (120- 400 m) and are interbedded with thick clay strata which generate different confinement grades. The interpretations made from 2H,18O and 3H results, as well as hydraulic calculations in the CAS, suggest that groundwater is old and that there is an age gradient increasing with depth and from W to SE. The obtained 14C ages for CAS A2 (3,700-1,100 BP) and CAS C (10,800 BP) indicate groundwater recharged during Holocene cold periods, between the 'Little Ice Age' (Isotope Stage 1, IS 1) and the ending of 'Holocene Climatic Optimum' (IS 2) and during the last glaciation, respectively. CAS D (46,100-40,500 BP) presents palaeowaters that could have been recharged during the Pleistocene (IS 3).","palabras_clave":"GROUNDWATER|AGE|ISOTOPIC|GEOCHEMICAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5117,"idioma_id":4,"titulo":"Association of thoracic aorta calcium and Non cardiac vascular events in cardiac disease-free individuals","resumen":"Objective- Thoracic aorta calcium (TAC) is measurable on the same computed tomography (CT) scan as coronary artery calcium (CAC) but has still unclear clinical value. We assessed TAC and CAC relations with non-cardiac vascular events history in a cohort of subjects at risk for cardiovascular disease.Methods- We analyzed retrospectively 1,000 consecutive subjects having undergone CAC detection by non-contrast multi-slice CT with measurement field longer than usual in order to measure total TAC including aortic arch calcium. We also determined partial TAC restricted to ascending and descending thoracic aorta sites by removing arch calcium from total TAC. Calcium deposits were measured with a custom made software using Agatston score. Results- Compared with the rest of the cohort, the 30 subjects with non-cardiac vascular event history had higher median values [95% CI] of total TAC (282 [28-1809] vs 39 [0-333], p&lt;0.01) and partial TAC (4 [0-284] vs 0 [0-5], p&lt;0.01) but no different value of CAC (73 [0-284] vs 16 [0-148]). Odds ratio [95% CI] of having non-cardiac vascular event per 1-SD increase in log-transformed calcium value was significant for total TAC but not for CAC, if total TAC and CAC were entered separately (1.56 [1.12-2.24], p&lt;0.01 and 1.13 [0.86-1.50], respectively) or together (1.57 [1.10-2.32], p&lt;0.01 and 0.98 [0.73-1.32], respectively) in the logistic adjusted model.Conclusion- TAC assessment simultaneous with CAC detection provides complementary information on the extra coronary component of cardiovascular risk beyond CAC's coronary risk prediction. Further studies are required to prospectively confirm this result.","palabras_clave":"CARDIOVASCULAR RISK|CEREBROVASCULAR DISEASE|CORONARY ARTERY CALCIUM|VASCULAR CALCIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5118,"idioma_id":4,"titulo":"Urinary bladder matrix scaffolds strengthen esophageal hiatus repair","resumen":"AbstractBACKGROUND:Laparoscopic repair of the hiatal hernia is associated with a recurrence rate between 12% and 42% depending on the defect size. Although the impact of hiatal reinforcement on long-term recurrence remains controversial, the main limitation of this approach has been the risk of adverse events related with the use of synthetic materials in the vicinity of the esophagus.METHODS:A total of 14 female domestic pigs underwent laparoscopic primary hiatal hernia repair of a simulated defect in the esophageal hiatus. Seven of the hiatal repairs were reinforced with an extracellular matrix (ECM) scaffold, whereas the remaining seven served as primary repair controls. Animals were survived for 8 wk. At necropsy, after gross morphologic evaluation, samples were sent for mechanical testing and histology.RESULTS:The repaired defect site reinforced with ECM scaffolds showed a robust closure of the crura in all cases with a smooth peritoneal-like structure covering the entire repair. Average load at failure of the treated group was found to be significantly stronger than that of the controls (185.8 ± 149.7 g versus 57.5 ± 57.5 g, P < 0.05). Similarly, the stiffness was significantly higher in the treated animals (57.5 ± 26.9 g\/mm versus 19.1 ± 17.5 g\/mm; P < 0.01). Interestingly, there was no difference in elongation at failure (7.62 ± 2.02 mm versus 7.87 ± 3.28 mm; P = 0.44).CONCLUSIONS:In our animal survival model, we have provided evidence that the addition of an ECM to augment a primary hiatal repair leads to tissue characteristics that may decrease the possibility of early failure of the repair. This may translate to decreased recurrence rates. Further study is necessary.","palabras_clave":"Hiatal hernia|Extracellular matrix|Hiatoplasty|Biologic scaffold"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5119,"idioma_id":4,"titulo":"ASSESSING INTERACTIONS BETWEEN GROUNDWATER AND SURFACE WATER IN A PAMPEAN STREAM","resumen":"In the south of Córdoba (Argentina), groundwater from the unconfined aquifer, presents scarce suitability due to high values of salts, sulfates, arsenic and fluorides. This situation makes it necessary to explore other possibilities, for example, conjunctive use of groundwater and surface water. The objective of this work was to describe the dynamic and geochemical relationships between surface water and groundwater in the medium-low basin of the Ají stream. The conceptual model was supported by the use of stable 18O\/16O and 2H\/1H isotopes and it was validated through numerical modeling and 222Rn activity results. The stream water is sodium bicarbonate fresh type (460?1,400 &#956;S\/cm). The aquifer presents fresh to salt water (720?14,000 &#956;S\/cm), from sodium bicarbonate to sodium chloride type. The isotopic results show that there is a straight mixing line between surface water and groundwater samples. The geochemical numerical modeling revealed that in the mixing models between stream and aquifer, for hydraulically linked samples, the aquifer only provides 2% of the total stream flow. This was validated through 222Rn results, which led us to the estimation of about 2% of groundwater supply in summer.","palabras_clave":"GROUNDWATER SURFICIAL WATER INTERACTION|ISOTOPE GEOCHEMISTRY|GEOCHEMICAL MODELLING|RADON"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5120,"idioma_id":4,"titulo":"Straightforward High-Pressure Synthesis and characterization of Indium-based Thio-Spinels: Photocatalytic potential for hydrogen production","resumen":"Ternary chalcogenides (AB2X4) based on the spinel structure are gaining a great deal of attention because of the possibility of tuning their magnetic and optoelectronic properties not only by changing chemical composition but also by altering their degree of inversion. Here we report a rapid high-pressure synthetic method for the synthesis of MIn2S4 powders starting from commercially available solid sulfides. We prove the versatility of our method by reporting the synthesis of six members of the MIn2S4 family (M = Mn, Fe, Co, Ni, Zn, and Cd) under high-pressure conditions (3.5 GPa); these compounds show complete to moderate degrees of inversion. Furthermore, this family covers a spectral region that includes visible band gaps. Interestingly, the structural refinement carried out by X-ray and neutron diffraction allows one to establish positive correlations between the gap and different parameters, including the degree of inversion. Finally, as a proof-of-concept, these ternary chalcogenides show moderate photocatalytic hydrogen production from aqueous solutions.","palabras_clave":"Synthetic methods|High-pressure chemistry|Photocatalysis|Dihydrogen|Chalcogens"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5121,"idioma_id":4,"titulo":"International perspectives on the effects of climate change on inland fisheries.","resumen":"Not available","palabras_clave":"Lakes and reservoirs|Ecology|Riparian and stream|Habtat modification|Fisheries|Management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5122,"idioma_id":4,"titulo":"BDNF isoforms: a round trip ticket between neurogenesis and serotonin?","resumen":"The Brain-Derived Neurotrophic Factor, BDNF, was discovered more than 30 years ago and, as the other members of the neurotrophin family, this neuropeptide is synthetized as a proneurotrophin, the proBDNF, which is further cleaved to yield mature BDNF. The myriad of actions of these two BDNF isoforms in the central nervous system is constantly increasing and requires the development of sophisticated tools and animal models to refine our understanding. This review is focused upon BDNF isoforms, their participation in the process of neurogenesis that takes place in the hippocampus of adult mammals, and the modulation of their expression by serotonergic agents. Interestingly, around this triumvirate of BDNF, serotonin and neurogenesis, there have been a series of recent research with apparently counterintuitive results that calls for an exhaustive analysis of the data published so far, and encourages thorough work in the quest for new hypothesis to the field.","palabras_clave":"BDNF isoforms|adult hippocampal neurogenesis|serotonergic system|rodents"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5123,"idioma_id":4,"titulo":"A new species of Adoxoplatys Breddin (Heteroptera: Pentatomidae: Discocephalinae) from Argentina","resumen":"The genus Adoxoplatys is distributed from Panama to Argentina. The eight included species are knownfrom a few specimens. In this contribution, a new species from Misiones Province, Argentina, Adoxoplatyssingularis sp. nov., is described and illustrated; and a key to identify the species of the genus is provided.","palabras_clave":"Adoxoplatys singularis|Misiones Province|new species|Ochlerini"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5124,"idioma_id":4,"titulo":"Species distribution models and local ecological knowledge in marine protected areas: The case of Os Miñarzos (Spain)","resumen":"Habitat suitability (HS) of target species is assessed in the coastal region of Seno de Corcubión, including Os Miñarzos Marine Protected Area (MPA) in west Galicia, NW of Spain. Current MPA was outlined using local environmental knowledge (LEK). Our objective is to test whether maximization of HS of 12 species would select the same MPA as fishers' LEK. A detailed capture database and 13 layers summarizing ecogeographic variables (derived from bathymetry, satellite data and sea bottom classification map) have been used and several Ecological Niche Factor Analysis (ENFA) Species Distribution Models (SDM) have been tested to find the best HS prediction (according to a nonparametric index due to Boyce). Our SDM results show that the LEK defined MPA (comprising 20% of the total area) is over 70% more suitable thanthe rest of the region for all species but one (European seabass, only 40%). We conclude that stakeholders LEK methodology correctly identifies local species habitats, and that SDM, and in particular ENFA models, can be used to validate and keep track of the MPA boundaries.","palabras_clave":"SPECIES DISTRIBUTION MODELS|ECOLOGICAL NICHE FACTOR ANALYSIS|MARINE PROTECTED AREAS|LOCAL ENVIRONMENTAL KNOWLEDGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5125,"idioma_id":4,"titulo":"Serum aminotransferases in nonalcoholic fatty liver disease are a signature of liver metabolic perturbations at the amino acid and Krebs cycle level","resumen":"BACKGROUND: Extensive epidemiologic studies have shown that cardiovascular disease and the metabolic syndrome (MetS) are associated with serum concentrations of liver enzymes; however, fundamental characteristics of this relation are currently unknown.OBJECTIVE: We aimed to explore the role of liver aminotransferases in nonalcoholic fatty liver disease (NAFLD) and MetS.DESIGN: Liver gene- and protein-expression changes of aminotransferases, including their corresponding isoforms, were evaluated in a case-control study of patients with NAFLD (n = 42), which was proven through a biopsy (control subjects: n = 10). We also carried out a serum targeted metabolite profiling to the glycolysis, gluconeogenesis, and Krebs cycle (n = 48) and an exploration by the next-generation sequencing of aminotransferase genes (n = 96). An in vitro study to provide a biological explanation of changes in the transcriptional level and enzymatic activity of aminotransferases was included.RESULTS: Fatty liver was associated with a deregulated liver expression of aminotransferases, which was unrelated to the disease severity. Metabolite profiling showed that serum aminotransferase concentrations are a signature of liver metabolic perturbations, particularly at the amino acid metabolism and Krebs cycle level. A significant and positive association between systolic hypertension and liver expression levels of glutamic-oxaloacetic transaminase 2 (GOT2) messenger RNA (Spearman R = 0.42, P = 0.03) was observed. The rs6993 located in the 3´ untranslated region of the GOT2 locus was significantly associated with features of the MetS, including arterial hypertension [P = 0.028; OR: 2.285 (95% CI: 1.024, 5.09); adjusted by NAFLD severity] and plasma lipid concentrations.CONCLUSIONS: ","palabras_clave":"NAFLD|aminotransferases|genes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5126,"idioma_id":4,"titulo":"Cover system for roofs manufactured with recycled polyethylene and rubber","resumen":"This Journal is indexed by Elsevier, SCOPUS, Ei Compendex (CPX), Cambridge Scientific Abstracts (CSA) Chemical Abstracts (CA), Google y Google Scholar google.com, ISI (ISTP, CPCI, Web of Science), Institución de Ingenieros Eléctricos Engineers (IEE), and Editor Publishing: Thomas Wohlbier, TTP EE.UU.  Abstract: The intense waste generation moved by the urban and industrial activity and the wasteful consumption of natural resources, encourage the development of new technologies for the utilization of wastes and its reuse in new industrial processes, which decreases the environmental pollution and the removal of virgin materials. In this sense we work in the development of new materials and components from urban and industrial waste prepared by different techniques and with various applications.  We work on the development of a composite material made with recycled rubber from tires out of use (NFU) and recycled polyethylene, linked through the fusion of the plastic and the subsequent pressing of the material. In this article we present the partial results obtained in the physical and mechanical testing of the roof tiles, and the burning behavior of the components manufactured with flame retardants additives available in the market, to comply with the regulations in order to use these housing components. These components have as main advantages the high resistance to flexion and the hard impact, low thermal conductivity, and low water absorption by immersion in water. Despite the fact that the components do not meet all the points of the reference Norm in regard to its behavior when exposed to fire, further amendments in the formulation and the manufacture process have shown interesting results, with which roof tiles could comply with the regulation for the use proposed.  We developed a sustainable covering system for roof using two types of wastes in great abundance in all urban sites, which involve a simple manufacture process. Besides, due to its high flexibility and resistance to impact hard is presented as a good constructive alternative for housing covering in areas affected by the fall of hail or by the risk of blowing up and deterioration by strong winds.","palabras_clave":"COVER SYSTEM|ROOF TILE|RECYCLED RUBBER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5127,"idioma_id":4,"titulo":"Inertial and dimensional effects on the instability of a thin film","resumen":"We consider here the effects of inertia on the instability of a flat liquid film under the effects of capillary and intermolecular forces (van der Waals interaction). Firstly, we perform the linear stability analysis within the long wave approximation, which shows that the inclusion of inertia does not produce new regions of instability other than the one previously known from the usual lubrication case. The wavelength, $lambda_m$, corresponding to he maximum growth, $omega_m$, and the critical (marginal) wavelength do not change at all. The most affected feature of the instability under an increase of the Laplace number is the noticeable decrease of the growth rates of the unstable modes. In order to put in evidence the effects of the bidimensional aspects of the flow(neglected in the long wave approximation), we also calculate the dispersion relation of the instability from the linearized version of the complete Navier-Stokes (N--S) equation. Unlike the long wave approximation, the bidimensional model shows that $lambda_m$ can vary significantly with inertia when the aspect ratio of the film is not sufficiently small. We also perform numerical simulations of the nonlinear N--S equations and analyze to which extent the linear predictions can be applied depending on both the amount ofinertia involved and the aspect ratio of the film.","palabras_clave":"INTERFACIAL FLOWS|LOW REYNOLDS NUMBERS FLOWS|LUBRICATION THEORY|THIN FILMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5128,"idioma_id":4,"titulo":"Metallic-thin-film instability with spatially correlated thermal noise","resumen":"We study the effects of stochastic thermal fluctuations on the instability of the free surface of a flat liquid metallic film upon a solid substrate. These fluctuations are represented by a stochastic noise term added to the deterministic equation for the film thickness within the long wave&nbsp; approximation. Unlike the case of polymeric films, we find that this noise, while remaining white in time, must be colored in space at least in some regimes. The corresponding noise term is characterized by a nonzero correlation length, $ell_c$, which combined with the size of the system, leads to a dimensionless parameter $eta$ that accounts for the relative importance of the spatial correlation ($eta sim ell_c^{-1}$). We perform the linear stability analysis (LSA) of the film both with and without the noise term, and find that for $ell_c$ larger than some critical value (depending on the system size), the wavelength of the peak of the spectrum is larger than that corresponding to the deterministic case, while for smaller $ell_c$ this peak corresponds to smaller wavelength than the latter. Interestingly, whatever the value of $ell_c$, the peak always approaches the deterministic one for larger times. We compare LSA results with the numerical simulations of the complete non-linear problem and find a good agreement in the power spectra for early times at different values of $eta$. For late times, we find that the stochastic LSA predicts well the position of the dominant wavelength, showing that nonlinear interactions do not modify the trends of the early linear stages. Finally, we fit the theoretical spectra to experimental data from a nanometric laser-melted copper film, and find that at later times, the adjustment requires smaller values of $eta$ (larger space correlations)","palabras_clave":"MICRO AND NANOSCALE FLOW|FLOW INSTABILITIES|STOCHASTIC ANALYSIS METHODS|FLUCTUATION PHENOMENA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5129,"idioma_id":4,"titulo":"Drops with non-circular footprints","resumen":"In this paper we study the morphology of drops formed on partially wetting substrates, whose footprint is not circular. These drops are&nbsp; consequence of the breakup processes occurring in thin films when anisotropic contact line motions take place. The anisotropy is basically due to the hysteresis of the contact angle since there is a wetting process in some parts of the contact line, while a deweting occurs in other parts. Here, we obtain a characteristic drop shape from the rupture of a long liquid filament sitting on a solid substrate. We analyze its shape and contact angles by means of goniometric and refractive techniques. We also find a non?trivial steady state solution for the drop shape within the long wave approximation (lubrication theory), and we compare most of itsfeatures with experimental data. This solution is presented both in&nbsp; Cartesian and polar coordinates, whose constants must be determined by a certain group of measured parameters. Besides, we obtain the dynamics of the drop generation from numerical simulations of the full Navier-Stokes equation, where we emulate the hysteretic effects with an appropriate spatial distribution of the static contact angle over the substrat.","palabras_clave":"DROPS|CONTACT ANGLE HYSTERESIS|THIN FILM FLOWS|WETTABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5130,"idioma_id":4,"titulo":"Frequency of Neospora caninum infections in beef cow-calf operations under extensive management","resumen":"Frequency of Neospora caninum infections in beefcow-calf operations under extensive management","palabras_clave":"NEOSPORA CANINUM|MANAGEMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5131,"idioma_id":4,"titulo":"Cyclooxygenase-2 expression in hepatocytes attenuates non-alcoholic steatohepatitis and liver fibrosis in mice","resumen":"p { margin-bottom: 0.25cm; line-height: 120%; }Cyclooxygenase-2(COX-2) is involved in different liver diseases but little is knownabout the significance of COX-2 in the development and progression ofnon-alcoholic steatohepatitis (NASH). This study was designed toelucidate the role of COX-2 expression in hepatocytes in thepathogenesis of steatohepatitis and hepatic fibrosis. In the presentwork, hepatocyte-specific COX-2 transgenic mice (hCOX-2-Tg) and theirwild-type (Wt) littermates were either fed methionine-and-cholinedeficient (MCD) diet to establish an experimental non-alcoholicsteatohepatitis (NASH) model or injected with carbon tetrachloride(CCl4) to induce liver fibrosis. In our animal model, hCOX-2-Tg micefed MCD diet showed lower grades of steatosis, ballooning andinflammation than Wt mice, in part by reduced recruitment andinfiltration of hepatic macrophages, with a corresponding decrease inserum levels of pro-inflammatory cytokines. Furthermore, hCOX-2-Tgmice showed a significant attenuation of the MCD diet-inducedincrease in oxidative stress and hepatic apoptosis observed in Wtmice. Even more, hCOX-2-Tg mice treated with CCl4 had significantlylower stages of fibrosis and less hepatic content of collagen,hydroxyproline and pro-fibrogenic markers than Wt controls.Collectively, our data indicates that constitutive hepatocyte COX-2expression ameliorates NASH and liver fibrosis development in mice byreducing inflammation, oxidative stress and apoptosis and bymodulating activation of hepatic stellate cells, respectively,suggesting a possible protective role for COX-2 induction inNASH\/NAFLD progression.p { margin-bottom: 0.25cm; line-height: 120%; Cyclooxygenase-2(COX-2) is involved in different liver diseases but little is knownabout the significance of COX-2 in the development and progression ofnon-alcoholic steatohepatitis (NASH). This study was designed toelucidate the role of COX-2 expression in hepatocytes in thepathogenesis of steatohepatitis and hepatic fibrosis. In the presentwork, hepatocyte-specific COX-2 transgenic mice (hCOX-2-Tg) and theirwild-type (Wt) littermates were either fed methionine-and-cholinedeficient (MCD) diet to establish an experimental non-alcoholicsteatohepatitis (NASH) model or injected with carbon tetrachloride(CCl4) to induce liver fibrosis. In our animal model,hCOX-2-Tg mice fed MCD diet showed lower grades of steatosis,ballooning and inflammation than Wt mice, in part by reducedrecruitment and infiltration of hepatic macrophages, with acorresponding decrease in serum levels of pro-inflammatory cytokines.Furthermore, hCOX-2-Tg mice showed a significant attenuation of theMCD diet-induced increase in oxidative stress and hepatic apoptosisobserved in Wt mice. Even more, hCOX-2-Tg mice treated with CCl4had significantly lower stages of fibrosis and less hepatic contentof collagen, hydroxyproline and pro-fibrogenic markers than Wtcontrols. Collectively, our data indicates that constitutivehepatocyte COX-2 expression ameliorates NASH and liver fibrosisdevelopment in mice by reducing inflammation, oxidative stress andapoptosis and by modulating activation of hepatic stellate cells,respectively, suggesting a possible protective role for COX-2induction in NASH\/NAFLD progression.&lt;select&gt;&lt;option value='af'&gt;Afrikaans&lt;\/option&gt;&lt;option value='sq'&gt;Albanian&lt;\/option&gt;&lt;option value='ar'&gt;Arabic&lt;\/option&gt;&lt;option value='hy'&gt;Armenian&lt;\/option&gt;&lt;option value='az'&gt;Azerbaijani&lt;\/option&gt;&lt;option value='eu'&gt;Basque&lt;\/option&gt;&lt;option value='be'&gt;Belarusian&lt;\/option&gt;&lt;option value='bg'&gt;Bulgarian&lt;\/option&gt;&lt;option value='ca'&gt;Catalan&lt;\/option&gt;&lt;option value='zh-CN'&gt;Chinese (Simplified)&lt;\/option&gt;&lt;option value='zh-TW'&gt;Chinese (Traditional)&lt;\/option&gt;&lt;option value='hr'&gt;Croatian&lt;\/option&gt;&lt;option value='cs'&gt;Czech&lt;\/option&gt;&lt;option value='da'&gt;Danish&lt;\/option&gt;&lt;option selected='da' value='auto'&gt;Detect language&lt;\/option&gt;&lt;option value='nl'&gt;Dutch&lt;\/option&gt;&lt;option value='en'&gt;English&lt;\/option&gt;&lt;option value='et'&gt;Estonian&lt;\/option&gt;&lt;option value='tl'&gt;Filipino&lt;\/option&gt;&lt;option value='fi'&gt;Finnish&lt;\/option&gt;&lt;option value='fr'&gt;French&lt;\/option&gt;&lt;option value='gl'&gt;Galician&lt;\/option&gt;&lt;option value='ka'&gt;Georgian&lt;\/option&gt;&lt;option value='de'&gt;German&lt;\/option&gt;&lt;option value='el'&gt;Greek&lt;\/option&gt;&lt;option value='ht'&gt;Haitian Creole&lt;\/option&gt;&lt;option value='iw'&gt;Hebrew&lt;\/option&gt;&lt;option value='hi'&gt;Hindi&lt;\/option&gt;&lt;option value='hu'&gt;Hungarian&lt;\/option&gt;&lt;option value='is'&gt;Icelandic&lt;\/option&gt;&lt;option value='id'&gt;Indonesian&lt;\/option&gt;&lt;option value='ga'&gt;Irish&lt;\/option&gt;&lt;option value='it'&gt;Italian&lt;\/option&gt;&lt;option value='ja'&gt;Japanese&lt;\/option&gt;&lt;option value='ko'&gt;Korean&lt;\/option&gt;&lt;option value='la'&gt;Latin&lt;\/option&gt;&lt;option value='lv'&gt;Latvian&lt;\/option&gt;&lt;option value='lt'&gt;Lithuanian&lt;\/option&gt;&lt;option value='mk'&gt;Macedonian&lt;\/option&gt;&lt;option value='ms'&gt;Malay&lt;\/option&gt;&lt;option value='mt'&gt;Maltese&lt;\/option&gt;&lt;option value='no'&gt;Norwegian&lt;\/option&gt;&lt;option value='fa'&gt;Persian&lt;\/option&gt;&lt;option value='pl'&gt;Polish&lt;\/option&gt;&lt;option value='pt'&gt;Portuguese&lt;\/option&gt;&lt;option value='ro'&gt;Romanian&lt;\/option&gt;&lt;option value='ru'&gt;Russian&lt;\/option&gt;&lt;option value='sr'&gt;Serbian&lt;\/option&gt;&lt;option value='sk'&gt;Slovak&lt;\/option&gt;&lt;option value='sl'&gt;Slovenian&lt;\/option&gt;&lt;option value='es'&gt;Spanish&lt;\/option&gt;&lt;option value='sw'&gt;Swahili&lt;\/option&gt;&lt;option value='sv'&gt;Swedish&lt;\/option&gt;&lt;option value='th'&gt;Thai&lt;\/option&gt;&lt;option value='tr'&gt;Turkish&lt;\/option&gt;&lt;option value='uk'&gt;Ukrainian&lt;\/option&gt;&lt;option value='ur'&gt;Urdu&lt;\/option&gt;&lt;option value='vi'&gt;Vietnamese&lt;\/option&gt;&lt;option value='cy'&gt;Welsh&lt;\/option&gt;&lt;option value='yi'&gt;Yiddish&lt;\/option&gt;&lt;\/select&gt;&lt;span style='font-weight: bold; cursor: pointer; color: lightgrey;'&gt;&#8644;&lt;\/span&gt;&lt;select&gt;&lt;option value='af'&gt;Afrikaans&lt;\/option&gt;&lt;option value='sq'&gt;Albanian&lt;\/option&gt;&lt;option value='ar'&gt;Arabic&lt;\/option&gt;&lt;option value='hy'&gt;Armenian&lt;\/option&gt;&lt;option value='az'&gt;Azerbaijani&lt;\/option&gt;&lt;option value='eu'&gt;Basque&lt;\/option&gt;&lt;option value='be'&gt;Belarusian&lt;\/option&gt;&lt;option value='bg'&gt;Bulgarian&lt;\/option&gt;&lt;option value='ca'&gt;Catalan&lt;\/option&gt;&lt;option value='zh-CN'&gt;Chinese (Simplified)&lt;\/option&gt;&lt;option value='zh-TW'&gt;Chinese (Traditional)&lt;\/option&gt;&lt;option value='hr'&gt;Croatian&lt;\/option&gt;&lt;option value='cs'&gt;Czech&lt;\/option&gt;&lt;option value='da'&gt;Danish&lt;\/option&gt;&lt;option value='nl'&gt;Dutch&lt;\/option&gt;&lt;option value='en'&gt;English&lt;\/option&gt;&lt;option value='et'&gt;Estonian&lt;\/option&gt;&lt;option value='tl'&gt;Filipino&lt;\/option&gt;&lt;option value='fi'&gt;Finnish&lt;\/option&gt;&lt;option value='fr'&gt;French&lt;\/option&gt;&lt;option value='gl'&gt;Galician&lt;\/option&gt;&lt;option value='ka'&gt;Georgian&lt;\/option&gt;&lt;option value='de'&gt;German&lt;\/option&gt;&lt;option value='el'&gt;Greek&lt;\/option&gt;&lt;option value='ht'&gt;Haitian Creole&lt;\/option&gt;&lt;option value='iw'&gt;Hebrew&lt;\/option&gt;&lt;option value='hi'&gt;Hindi&lt;\/option&gt;&lt;option selected='hi' value='hu'&gt;Hungarian&lt;\/option&gt;&lt;option value='is'&gt;Icelandic&lt;\/option&gt;&lt;option value='id'&gt;Indonesian&lt;\/option&gt;&lt;option value='ga'&gt;Irish&lt;\/option&gt;&lt;option value='it'&gt;Italian&lt;\/option&gt;&lt;option value='ja'&gt;Japanese&lt;\/option&gt;&lt;option value='ko'&gt;Korean&lt;\/option&gt;&lt;option value='la'&gt;Latin&lt;\/option&gt;&lt;option value='lv'&gt;Latvian&lt;\/option&gt;&lt;option value='lt'&gt;Lithuanian&lt;\/option&gt;&lt;option value='mk'&gt;Macedonian&lt;\/option&gt;&lt;option value='ms'&gt;Malay&lt;\/option&gt;&lt;option value='mt'&gt;Maltese&lt;\/option&gt;&lt;option value='no'&gt;Norwegian&lt;\/option&gt;&lt;option value='fa'&gt;Persian&lt;\/option&gt;&lt;option value='pl'&gt;Polish&lt;\/option&gt;&lt;option value='pt'&gt;Portuguese&lt;\/option&gt;&lt;option value='ro'&gt;Romanian&lt;\/option&gt;&lt;option value='ru'&gt;Russian&lt;\/option&gt;&lt;option value='sr'&gt;Serbian&lt;\/option&gt;&lt;option value='sk'&gt;Slovak&lt;\/option&gt;&lt;option value='sl'&gt;Slovenian&lt;\/option&gt;&lt;option value='es'&gt;Spanish&lt;\/option&gt;&lt;option value='sw'&gt;Swahili&lt;\/option&gt;&lt;option value='sv'&gt;Swedish&lt;\/option&gt;&lt;option value='th'&gt;Thai&lt;\/option&gt;&lt;option value='tr'&gt;Turkish&lt;\/option&gt;&lt;option value='uk'&gt;Ukrainian&lt;\/option&gt;&lt;option value='ur'&gt;Urdu&lt;\/option&gt;&lt;option value='vi'&gt;Vietnamese&lt;\/option&gt;&lt;option value='cy'&gt;Welsh&lt;\/option&gt;&lt;option value='yi'&gt;Yiddish&lt;\/option&gt;&lt;\/select&gt;&lt;div style='text-align: left; background-color: rgb(235, 239, 249);'&gt;Detect language » Hungarian&lt;\/div&gt;&lt;div style='width: 444px; max-width: 444px; padding: 2px; min-height: 80px; border-width: 1px; border-style: solid; border-color: grey; background-color: rgb(255, 255, 255); text-align: justify;' \/&gt;&lt;\/div&gt;&lt;\/body&gt;&lt;\/html&gt;&lt;select&gt;&lt;option value='af'&gt;Afrikaans&lt;\/option&gt;&lt;option value='sq'&gt;Albanian&lt;\/option&gt;&lt;option value='ar'&gt;Arabic&lt;\/option&gt;&lt;option value='hy'&gt;Armenian&lt;\/option&gt;&lt;option value='az'&gt;Azerbaijani&lt;\/option&gt;&lt;option value='eu'&gt;Basque&lt;\/option&gt;&lt;option value='be'&gt;Belarusian&lt;\/option&gt;&lt;option value='bg'&gt;Bulgarian&lt;\/option&gt;&lt;option value='ca'&gt;Catalan&lt;\/option&gt;&lt;option value='zh-CN'&gt;Chinese (Simplified)&lt;\/option&gt;&lt;option value='zh-TW'&gt;Chinese (Traditional)&lt;\/option&gt;&lt;option value='hr'&gt;Croatian&lt;\/option&gt;&lt;option value='cs'&gt;Czech&lt;\/option&gt;&lt;option value='da'&gt;Danish&lt;\/option&gt;&lt;option selected='da' value='auto'&gt;Detect language&lt;\/option&gt;&lt;option value='nl'&gt;Dutch&lt;\/option&gt;&lt;option value='en'&gt;English&lt;\/option&gt;&lt;option value='et'&gt;Estonian&lt;\/option&gt;&lt;option value='tl'&gt;Filipino&lt;\/option&gt;&lt;option value='fi'&gt;Finnish&lt;\/option&gt;&lt;option value='fr'&gt;French&lt;\/option&gt;&lt;option value='gl'&gt;Galician&lt;\/option&gt;&lt;option value='ka'&gt;Georgian&lt;\/option&gt;&lt;option value='de'&gt;German&lt;\/option&gt;&lt;option value='el'&gt;Greek&lt;\/option&gt;&lt;option value='ht'&gt;Haitian Creole&lt;\/option&gt;&lt;option value='iw'&gt;Hebrew&lt;\/option&gt;&lt;option value='hi'&gt;Hindi&lt;\/option&gt;&lt;option value='hu'&gt;Hungarian&lt;\/option&gt;&lt;option value='is'&gt;Icelandic&lt;\/option&gt;&lt;option value='id'&gt;Indonesian&lt;\/option&gt;&lt;option value='ga'&gt;Irish&lt;\/option&gt;&lt;option value='it'&gt;Italian&lt;\/option&gt;&lt;option value='ja'&gt;Japanese&lt;\/option&gt;&lt;option value='ko'&gt;Korean&lt;\/option&gt;&lt;option value='la'&gt;Latin&lt;\/option&gt;&lt;option value='lv'&gt;Latvian&lt;\/option&gt;&lt;option value='lt'&gt;Lithuanian&lt;\/option&gt;&lt;option value='mk'&gt;Macedonian&lt;\/option&gt;&lt;option value='ms'&gt;Malay&lt;\/option&gt;&lt;option value='mt'&gt;Maltese&lt;\/option&gt;&lt;option value='no'&gt;Norwegian&lt;\/option&gt;&lt;option value='fa'&gt;Persian&lt;\/option&gt;&lt;option value='pl'&gt;Polish&lt;\/option&gt;&lt;option value='pt'&gt;Portuguese&lt;\/option&gt;&lt;option value='ro'&gt;Romanian&lt;\/option&gt;&lt;option value='ru'&gt;Russian&lt;\/option&gt;&lt;option value='sr'&gt;Serbian&lt;\/option&gt;&lt;option value='sk'&gt;Slovak&lt;\/option&gt;&lt;option value='sl'&gt;Slovenian&lt;\/option&gt;&lt;option value='es'&gt;Spanish&lt;\/option&gt;&lt;option value='sw'&gt;Swahili&lt;\/option&gt;&lt;option value='sv'&gt;Swedish&lt;\/option&gt;&lt;option value='th'&gt;Thai&lt;\/option&gt;&lt;option value='tr'&gt;Turkish&lt;\/option&gt;&lt;option value='uk'&gt;Ukrainian&lt;\/option&gt;&lt;option value='ur'&gt;Urdu&lt;\/option&gt;&lt;option value='vi'&gt;Vietnamese&lt;\/option&gt;&lt;option value='cy'&gt;Welsh&lt;\/option&gt;&lt;option value='yi'&gt;Yiddish&lt;\/option&gt;&lt;\/select&gt;&lt;span style='font-weight: bold; cursor: pointer; color: lightgrey;'&gt;&#8644;&lt;\/span&gt;&lt;select&gt;&lt;option value='af'&gt;Afrikaans&lt;\/option&gt;&lt;option value='sq'&gt;Albanian&lt;\/option&gt;&lt;option value='ar'&gt;Arabic&lt;\/option&gt;&lt;option value='hy'&gt;Armenian&lt;\/option&gt;&lt;option value='az'&gt;Azerbaijani&lt;\/option&gt;&lt;option value='eu'&gt;Basque&lt;\/option&gt;&lt;option value='be'&gt;Belarusian&lt;\/option&gt;&lt;option value='bg'&gt;Bulgarian&lt;\/option&gt;&lt;option value='ca'&gt;Catalan&lt;\/option&gt;&lt;option value='zh-CN'&gt;Chinese (Simplified)&lt;\/option&gt;&lt;option value='zh-TW'&gt;Chinese (Traditional)&lt;\/option&gt;&lt;option value='hr'&gt;Croatian&lt;\/option&gt;&lt;option value='cs'&gt;Czech&lt;\/option&gt;&lt;option value='da'&gt;Danish&lt;\/option&gt;&lt;option value='nl'&gt;Dutch&lt;\/option&gt;&lt;option value='en'&gt;English&lt;\/option&gt;&lt;option value='et'&gt;Estonian&lt;\/option&gt;&lt;option value='tl'&gt;Filipino&lt;\/option&gt;&lt;option value='fi'&gt;Finnish&lt;\/option&gt;&lt;option value='fr'&gt;French&lt;\/option&gt;&lt;option value='gl'&gt;Galician&lt;\/option&gt;&lt;option value='ka'&gt;Georgian&lt;\/option&gt;&lt;option value='de'&gt;German&lt;\/option&gt;&lt;option value='el'&gt;Greek&lt;\/option&gt;&lt;option value='ht'&gt;Haitian Creole&lt;\/option&gt;&lt;option value='iw'&gt;Hebrew&lt;\/option&gt;&lt;option value='hi'&gt;Hindi&lt;\/option&gt;&lt;option selected='hi' value='hu'&gt;Hungarian&lt;\/option&gt;&lt;option value='is'&gt;Icelandic&lt;\/option&gt;&lt;option value='id'&gt;Indonesian&lt;\/option&gt;&lt;option value='ga'&gt;Irish&lt;\/option&gt;&lt;option value='it'&gt;Italian&lt;\/option&gt;&lt;option value='ja'&gt;Japanese&lt;\/option&gt;&lt;option value='ko'&gt;Korean&lt;\/option&gt;&lt;option value='la'&gt;Latin&lt;\/option&gt;&lt;option value='lv'&gt;Latvian&lt;\/option&gt;&lt;option value='lt'&gt;Lithuanian&lt;\/option&gt;&lt;option value='mk'&gt;Macedonian&lt;\/option&gt;&lt;option value='ms'&gt;Malay&lt;\/option&gt;&lt;option value='mt'&gt;Maltese&lt;\/option&gt;&lt;option value='no'&gt;Norwegian&lt;\/option&gt;&lt;option value='fa'&gt;Persian&lt;\/option&gt;&lt;option value='pl'&gt;Polish&lt;\/option&gt;&lt;option value='pt'&gt;Portuguese&lt;\/option&gt;&lt;option value='ro'&gt;Romanian&lt;\/option&gt;&lt;option value='ru'&gt;Russian&lt;\/option&gt;&lt;option value='sr'&gt;Serbian&lt;\/option&gt;&lt;option value='sk'&gt;Slovak&lt;\/option&gt;&lt;option value='sl'&gt;Slovenian&lt;\/option&gt;&lt;option value='es'&gt;Spanish&lt;\/option&gt;&lt;option value='sw'&gt;Swahili&lt;\/option&gt;&lt;option value='sv'&gt;Swedish&lt;\/option&gt;&lt;option value='th'&gt;Thai&lt;\/option&gt;&lt;option value='tr'&gt;Turkish&lt;\/option&gt;&lt;option value='uk'&gt;Ukrainian&lt;\/option&gt;&lt;option value='ur'&gt;Urdu&lt;\/option&gt;&lt;option value='vi'&gt;Vietnamese&lt;\/option&gt;&lt;option value='cy'&gt;Welsh&lt;\/option&gt;&lt;option value='yi'&gt;Yiddish&lt;\/option&gt;&lt;\/select&gt;&lt;div style='text-align: left; background-color: rgb(235, 239, 249);'&gt;Detect language » Hungarian&lt;\/div&gt;&lt;div style='width: 444px; max-width: 444px; padding: 2px; min-height: 80px; border-width: 1px; border-style: solid; border-color: grey; background-color: rgb(255, 255, 255); text-align: justify;' \/&gt;&lt;\/div&gt;&lt;\/body&gt;&lt;\/html&gt;AfrikaansAlbanianArabicArmenianAzerbaijaniBasqueBelarusianBulgarianCatalanChinese (Simplified)Chinese (Traditional)CroatianCzechDanishDetect languageDutchEnglishEstonianFilipinoFinnishFrenchGalicianGeorgianGermanGreekHaitian CreoleHebrewHindiHungarianIcelandicIndonesianIrishItalianJapaneseKoreanLatinLatvianLithuanianMacedonianMalayMalteseNorwegianPersianPolishPortugueseRomanianRussianSerbianSlovakSlovenianSpanishSwahiliSwedishThaiTurkishUkrainianUrduVietnameseWelshYiddish&#8644;AfrikaansAlbanianArabicArmenianAzerbaijaniBasqueBelarusianBulgarianCatalanChinese (Simplified)Chinese (Traditional)CroatianCzechDanishDutchEnglishEstonianFilipinoFinnishFrenchGalicianGeorgianGermanGreekHaitian CreoleHebrewHindiHungarianIcelandicIndonesianIrishItalianJapaneseKoreanLatinLatvianLithuanianMacedonianMalayMalteseNorwegianPersianPolishPortugueseRomanianRussianSerbianSlovakSlovenianSpanishSwahiliSwedishThaiTurkishUkrainianUrduVietnameseWelshYiddishDetect language » Hungarian","palabras_clave":"COX-2|Fibrosis|Inflammation|Liver|Steatohepatitis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5132,"idioma_id":4,"titulo":"Neo-sex Chromosomes in the Maculipennis Species Group  (Dichroplus: Acrididae, Melanoplinae): The Cases of D. maculipennis and D. vittigerum","resumen":"South American melanopline grasshoppers display a disproportionate number of derived karyotypes including many cases of neo-sex chromosome systems. This is especially true of the genus Dichroplus and its Maculipennis species group. We analyzed the karyotype and neo-sex chromosomes in mitosis and meiosis of Dichroplus maculipennis and D.vittigerum from Argentina using conventional and fluorescent cytogenetic protocols in order to elucidate the behavior and origin of these neo-XY systems in relation to the current phylogeny of this group. Our results showed that D. maculipennis (2n= 22&#9794;\/22&#9792;; neoXY\/neoXX) and D. Vittigerum, whose karyotype is described for the first time (2n=18&#9794;\/18&#9792;; neoXY\/neoXX) show highly evolved neo-XY systems although with significant differences between them. Furthermore, both species differ for two autosomalfixed Robertsonian fusions present in D. vittigerum. Analysis of karyotypic character state optimization strongly suggests the independent origin and evolution of neo-sex systems within this species group.","palabras_clave":"DICHROPLUS MACULIPENNIS|DICHROPLUS VITTIGERUM|GRASSHOPPER|KARYOTYPE CHARACTER STATE OPTIMIZATION|MELANOPLINAE|SEX CHROMOSOMES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5133,"idioma_id":4,"titulo":"Functional VO2 considerations on young tennis players","resumen":"Las características intermitentes del Tenis, caracterizado por esfuerzos intensos de corta y muy corta duración, aunque repetidos durante varias horas de juego, junto a las pausas reglamentarias fijas entre tantos y al final de cada set, justifican la importancia del estudio de la cinética del consumo de oxígeno (VO2) para describir acontecimientos funcionales y metabólicos que ocurren durante un partido. El objetivo fué describir la cinética del VO2 de tenistas juveniles de elite, en  laboratorio, y en una situación de juego, observando la relación entre los valores máximos y los utilizados en campo. Se evaluaron 10 jugadores de tenis masculinos de 12 a 22 años, en una prueba máxima de laboratorio, y en una situación de real de juego (primer set, 6 games). En laboratorio, se determinó: consumo máximo de oxígeno (VO2 max),  producción de dióxido de carbono (VCO2 max),  ventilación (VE), cociente respiratorio (RER), frecuencia cardíaca (FC) y umbral anaeróbico (AT). En campo, se midió el VO2, el VCO2  y la FC de manera contínua; y se calcularon distintos indicadores relacionando los datos de laboratorio con los de campo. Durante el  juego, el porcentaje de VO2 max promedio comprometido fue del 77,75% (SD±4,5), el cual se relacionó con el valor del umbral anaeróbico (95,56% (SD±6,4)). Se concluye sobre la importancia de la aptitud aeróbica en el tenis, caracterizado por situaciones anaeróbicas pero repetidas a lo largo de varias horas de juego. Se recomienda que en la preparación física, se desarrolle adecuadamente la condición aeróbica.","palabras_clave":"Consumo de oxígeno|Tenistas juveniles|VO2 peak|Evaluación en partido"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5134,"idioma_id":4,"titulo":"Characterization of biodegradable films based on Salvia hispanica L protein and mucilage","resumen":"Biodegradable films of chia by-products (mucilage and protein-rich fraction (PF)) incorporated with clove essential oil (CEO) were obtained and characterized. The effects of polymer concentration (PC; 1.0?3.0 %,w\/v) and CEO concentration (0.1?1.0 %,v\/v) were evaluated as well as the pH (7?10), using a 2^3 factorial design with four central points. The films exhibited moisture values between 11.6 and 52.1 % (d.b.), which decreased (p&lt; 0.05) with increasing PCand CEO. The thickness of the films increased (p&lt; 0.05) with increasing PC. PC and pH influenced (p&lt; 0.05) the lightness (L) and variation in color between red and green (a). Theorientation of the color to yellow-blue hues (b) decreased significantly (p&lt; 0.05) with increasing PC. Transparency was significantly lower and higher (p&lt; 0.05) than PC and CEO, respectively. The film surface morphology was evaluated using atomic force miscrocope images, and thermogravimetric analysis (TGA) was performed to study the thermal stability of the films. The displacement and tensile strength were significantly lower (p&lt; 0.05) at higher concentrations of CEO, this variable being the only one with a significant effect. The chemical composition of the films was confirmed utilizing Fourier transform infrared (FTIR) spectroscopy. The proportion of CEO added to the films had a significant influence on antimicrobial activity, inhibiting the growth of both Escherichia coli and Staphylococcus aureus.","palabras_clave":"BIODEGRADABLE FILMS|PHYSICAL CHARACTERIZATION|MECHANICAL PROPERTIES|ANTIBACTERIAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5135,"idioma_id":4,"titulo":"Stability of oil-in-water (O\/W) emulsions with chia (Salvia hispanica L.) mucilage","resumen":"The stability of oil-in-water (O\/W) emulsions prepared with different concentrations of chia mucilage extracted by two different methods was evaluated as a function of refrigerated storage time. O\/W dispersions (0.25, 0.50, 0.75 and 1.00% wt\/wt) and 0.1% wt\/wt of Tween 80 were stored at 4 ± 1 C for 120 days, evaluating their stability periodically. An emulsion without added mucilage was also prepared as a control. The emulsions were characterized determining the flow behavior, the evolution of the backscattering profiles (%BS), particlesize distribution, and microscopic observations. Viscosity increased as increasing mucilage concentration, revealing a pseudoplastic flow behavior. The most stable emulsions during storage were those with 0.75% chia mucilage concentration, and those with mucilage obtained by Method II (which presents ahigher level of purity than MI mucilage). All the emulsions prepared with mucilage obtained by Method II presented smaller droplet diameters ([D4,3]) than those prepared with mucilage obtained by Method I. Particle size distribution did not show important variations as a function of time, concentration and typeof mucilage, presenting in general a unimodal behavior. The results suggested that the addition of chia mucilage (0.75%) to O\/W emulsions improved their stability against gravitational phase separation by increasing the viscosity of the aqueous phase, limiting the mobility of the oil droplets in the emulsions.","palabras_clave":"Salvia hispanica L.|Chia mucilage |O\/W emulsions |Physicochemical stability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5136,"idioma_id":4,"titulo":"The embedded clusters DBS 77, 78, 102 and 106-161 and their link with the interstellar medium","resumen":"Aims. We report a study of the global properties of some embedded clusters placed in the fourth quadrant of the Milky Way to clarify some issues related with their location into de Galaxy and their stellar formation processes.Methods. We performed BVI photometric observations in the region of DBS 77, 78, 102, 160, and 161 clusters and infrared spectroscopy in DBS 77 region. They were complemented with JHK data from VVV survey combined with 2MASS catalogue, and used mid-infrared information from GLIMPSE catalogue. We also searched for HI data from SGPS and PMN radio surveys, and previous spectroscopic stellar classification. The spectroscopic and photometric information allowed us to estimate the spectral classification of the brightest stars of each studied region. On the other hand, we used the radio data to investigate the interstellar material parameters and the continuum sources probably associated with the respective stellar components.Results. We estimated the basic physical parameters of the clusters (reddening, distance, age, and initial mass function).We searched for HII regions located near to the studied clusters and we analyzed the possible link between them. In the particular case of DBS 160&#1048576;161 clusters, we identified the HI bubble B332.5-0.1-42 located around them.We found that the mechanical energy injected to the interstellar medium by the more massive stars of this couple of clusters was enough to generate the bubble.","palabras_clave":"Stars: early-type|Stars: pre-main sequence|Stars: formation|ISM: structure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5137,"idioma_id":4,"titulo":"EFFECT OF ACTIVATION ATMOSPHERE IN THE FISCHER?TROPSCH SYNTHESIS USING A ??QUASI-MODEL? CATALYST OF &#61543;-Fe2O3 NANOPARTICLES SUPPORTED ON SBA-15","resumen":"The effect of different activation atmospheres (CO:H2 or pure H2) on activity and selectivity of ironnanoparticles supported on SBA-15 in Fischer?Tropsch Synthesis, has been studied. To achieve thisaim a new preparation method, using monodisperse pre-synthesized c-Fe2O3 nanoparticles of 3 nm size,supported on SBA-15, was used. These catalysts have structural properties between real and modelcatalysts. Therefore, they were named ??quasi-model? catalysts. Nanoparticles and catalysts werecharacterized with several techniques: XRD, N2 adsorption at 77 K, magnetic measurements, electrontransmission microscopy, Mössbauer spectroscopy between 298 and 13 K in air and controlledatmosphere and thermal gravimetric analysis. Catalytic tests showed clearly that activation with pure H2leads to a catalyst more active and less selective to methane than that activated with CO:H2.To explain these results, different reduction steps were proposed. These different sequences would producea diverse number of active sites.","palabras_clave":"'QUASI-MODEL' CATALYSTS|IRON OXIDES NANOPARTICLES|FISHER-TROPSCH SYNTHESIS|FE\/SBA-15 SYSTEM|ACTIVATION ATMOSPHERE EFFECT|MOSSBAUER SPECTROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5138,"idioma_id":10,"titulo":"Novel cruzipain inhibitors for the chemotherapy of chronic Chagas disease","resumen":"In spite of current efforts worldwide to develop new medications against Chagas disease, only two drugs are available, nifurtimox and benznidazole. Both drugs require prolonged treatment, have multiple side effects and limited efficacy on adult patients chronically infected with Trypanosoma cruzi. Recently, computer-guided drug repositioning led to discovery of the trypanocidal effects of clofazimine and benidipine. These compounds showed inhibitory effects on  cruzipain, the major cysteine protease of the parasite, on different T. cruzi stages and in a mouse model of acute Chagas disease. The aim of this work was to test the efficacy of these novel cruzipain inhibitors when administered to a model of chronic Chagas disease. Benidipine and clofazimine were able to reduce the parasite burden in cardiac and skeletal muscle of chronically infected mice compared to the untreated ones, and diminished the inflammatory process on these tissues. Further studies should be performed to study the synergism with benznidazole and nifurtimox in view of combined therapies.","palabras_clave":"CHAGAS DISEASE|TRYPANOSOMA CRUZI|CHRONIC PHASE|DRUG REPOSITIONING|BENIDIPINE|CLOFAZIMINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5139,"idioma_id":4,"titulo":"A SELECTIVE GENETIC ALGORITHM FOR MULTIOBJECTIVE OPTIMIZATION OF CROSS SECTIONS IN 3D TRUSSED STRUCTURES BASED ON A SPATIAL SENSITIVITY ANALYSIS","resumen":"There are many problems in civil or mechanical engineering related to structural design. In such a case the solution techniques which lead to deterministic results are no longer valid due to the heuristic nature of design problems. In this article a computational tool based on genetic algorithms, applied to the optimal design of cross sections (solid tubes) of 3D truss structures is proposed. The main feature of this genetic algorithm approach is the introduction of a selective-smart method developed in order to improve the convergence rate of large optimization problems. This selective genetic algorithm is based on a preliminary sensitivity analysis performed over each variable, in order to reduce the search space of the evolutionary process. In order to account for the optimization of the total weight, the displacement (of a specific section) and the internal stresses distribution of the structure a multiobjective optimization function was proposed. The numerical results presented in this article show a significant improvement in the convergence rate as well as an important reduction in the relative error, compared to the exact solution.","palabras_clave":"GENETIC ALGORITHM|MULTIOJECTIVE OPTIMIZACION|SENSITIVITY ANALYSIS|TRUSS STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5140,"idioma_id":4,"titulo":"Incidence of Sperm Surface Autoantibodies and Relationship with Routine Semen Parameters and Sperm Kinematics","resumen":"Incidence of sperm surface autoantibodies and relationship with routine semen parameters and sperm kinematics. Verón GL, Molina RI, Tissera AD, Estofan GM, Marín Briggiler CI*, Vazquez-Levin MH*. (*IGUAL CONTRIBUCION). ABSTRACT PROBLEM: AntiSperm Antibodies (ASA) are associated with male subfertility. However, results on sperm surface-bound antibodies are controversial, the relationship between ASA and semen parameters (WHO 2010) is unknown, and data on ASA and sperm kinematics are scarce. METHOD OF STUDY: A retrospective study done in men undergoing routine semen analysis (WHO 2010), ASA evaluation (direct SpermMARTM (IgG) Test) and Computer-Assisted Sperm Analysis (CASA).RESULTS: A 2.6% and a 5.9% incidence of ASA-positive cases was found (cut-off 50% and 10%, respectively; n=7492). ASA-positive samples had lower (P<0.0001) sperm concentration, count, motility and Hypo-Osmotic Swelling (HOS) Test score. HOS scores did not correlate with sperm vitality in normozoospermic samples with high ASA levels. The presence of ASA (cut-off 50%) was related to decreased sperm kinematics (VSL, VAP, LIN, ALH, STR, BCF, WOB). In contrast, normozoospermic samples showed comparable CASA results in ASA-positive and negative subgroups. CONCLUSIONS: ASA evaluation is highly relevant in full semen assessments.","palabras_clave":"antisperm antibody|semen parameters|CASA|hypo-osmotic test"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5141,"idioma_id":5,"titulo":"De la vida, la muerte y otras trivialidades","resumen":"En este ensayo se analizaron tres diferentes modelos de prácticas rituales alrededor de la muerte en producciones audiovisuales contemporáneas. Las autoras plantearon como punto común la estructura ficcional de la realidad, o sea entenderla como la construcción de una narración alrededor de una verdad indecible como en este caso es la de la muerte. Los casos de estudio elegidos fueron tres tematizaciones diferentes ?que se transforman en prácticas performativas? acerca de la relación del hombre con la finitud: los velorios simbólicos efectuados por los hijos de desaparecidos que nunca recuperaron los cuerpos de sus padres, los dos films de Cesar González Diagnóstico esperanza (2013) y ¿Qué puede un cuerpo? (2014) en donde se analiza los ritos en torno a la muerte. Y por último, en el capítulo siete del anime Kanamemo (2009), se estudió el ritual de conmemoración de los muertos a través de la celebración del Obon, una costumbre de origen budista que se ha arraigado en la cultura japonesa. Las tres autoras realizan un análisis comparativo en búsqueda de regularidades y rupturas que den cuenta del entramado de relaciones e influencias culturales, políticas, religiosas y el papel de las industrias culturales en las representaciones de la muerte.","palabras_clave":"MUERTE|REPRESENTACIONES|PRÁCTICAS RITUALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5142,"idioma_id":5,"titulo":"Petrografía y geoquímica del cuerpo ígneo El Renegado, Sierra Grande de San Luis","resumen":"El Renegado es un cuerpo ígneo situado en el sector Sur de la faja derocas máficasultramáficas La Jovita-Las Águilas emplazada en el faldeo orientalde la Sierra Grande de San Luis, la cual se extiende aproximadamente 100 km conrumbo NNE, con un ancho que oscila entre 3 y 5 km. Si bien este cuerpo carecede estudios previos a la presente contribución, se encuentra próximo a diversoscuerpos máficos-ultramáficos ampliamente estudiados tales como Las Águilas, LasHigueras, Virorco, Escuela Las Pircas y El Fierro, los cuales son de graninterés por ser portadores de una mineralización de sulfuros de Fe-Ni-Cu-Co yminerales del grupo del platino. El Renegado es un cuerpo de composiciónmáfica, presentando en diversos sectores textura magmática primaria de tipoestratificada modal. Los resultados de los análisis geoquímicos de elementosmayoritarios, elementos traza y elementos de tierras raras, permitieron definirpara El Renegado un proceso de evolución magmática por cristalizaciónfraccionada, con un grado de evolución que aumenta de este a oeste. Cuandoestos resultados son comparados mediante diagramas de elementos mayoritarios,elementos traza y elementos de tierras raras con los cuerposmáficosultramáficos Virorco, Las Águilas y Las Higueras, es posible determinarque todos ellos pertenecen a una misma secuencia magmática controlada porcristalización fraccionada, en la cual las rocas aflorantes en El Renegadorepresentarían las de mayor grado de evolución dentro de esta secuenciaestratigráfica ígnea.","palabras_clave":"Máficas-ultramáficas|estratificación magmática|Cristalización fraccionada|Sierras Pampeanas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5143,"idioma_id":4,"titulo":"Valorization of Rhizoclonium sp. algae via pyrolysis and catalytic pyrolysis","resumen":"The valorization of Rhizoclonium sp. algae through pyrolysis for obtaining bio-oils is studied in this work.The reaction is carried out at 400 C, at high contact time. The bio-oil has a practical yield of 35% and isrich in phytol. Besides, it is simpler than the corresponding to lignocellulosic biomass due to the absenceof phenolic compounds. This property leads to a bio-oil relatively stable to storage. In addition,heterogeneous catalysts (Al?Fe\/MCM-41, SBA-15 and Cu\/SBA-15), in contact with algae during pyrolysis,are analyzed. The general trend is that the catalysts decrease the concentration of fatty alcohols and otherhigh molecular weight products, since their mild acidity sites promote degradation reactions. Thus, theamount of light products increases upon the use of the catalysts. Particularly, acetol concentration inthe bio-oils obtained from the catalytic pyrolysis with SBA-15 and Cu\/SBA-15 is notably high.","palabras_clave":"Algal biomass|Phyto|Catalytic pyrolysis|Bio-oils"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5144,"idioma_id":4,"titulo":"Combined high hydrostatic pressure and thermal treatments fully inactivate trypsin inhibitors and lipoxygenase and improve protein solubility and physical stability of calcium-added soymilk","resumen":"The objective of this work was to assess the possibility of obtaining calcium-added soymilk with acceptable characteristics regarding protein solubility and physical stability, and inactivation of trypsin inhibitors and lipoxygenase, through a combined thermal-high hydrostatic pressure treatment. A Doehlert design was applied to study the effect of combining pressure levels (500?700 MPa), initial temperatures (45?65 °C) and CaCl2 concentrations (5?15 mmol L&#8722;1). Results showed that protein solubility was a function of CaCl2 concentration (p < 0.005), and that inactivation of trypsin inhibitors was a function of pressure level and temperature (p < 0.005). Lipoxygenase activity was fully inactivated in most of the conditions tested. Physical stability was improved by the combined treatments: depending on calcium concentration, either no settling was detected in a 5-day period or a less conspicuous phase separation was observed. Our results indicate that some combined thermal-high hydrostatic pressure treatments allow the preparation of calcium-enriched soymilks with improved physical stability without additives such as chelating agents, and acceptable in terms of full inactivation of trypsin inhibitors and lipoxygenase. Industrial relevance: An analysis of the effect of the combination of high hydrostatic and thermal treatments on calcium-added soymilk is introduced in the present study. Some processing conditions were found to allow the obtention of a product with improved protein solubility and physical stability, and with inactivated trypsin inhibitors and lipoxygenase enzyme. This information could be bene&#64257;cial considering the reduction of process time and energetic costs, because the assayed combination of thermal and high pressure treatments takes advantage of the instantaneous compression heating. Thus, the results provided in the present work could be useful to prepare an acceptable calcium-added soymilk.","palabras_clave":"HIGH HYDROSTATIC PRESSURE|CALCIUM|SOLUBILITY|SOYMILK"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5145,"idioma_id":4,"titulo":"Comparative study on the in vitro replication and genomic variability of Argentinean field isolates of bovine herpesvirus type 4 (BoHV-4)","resumen":"Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus, belonging to the Rhadinovirus genus, which is increasingly associated with various problems of the reproductive tract of cattle. In Argentina, analysis of BoHV-4 strains isolated from cervico-vaginal mucus of aborted cows revealed a high genetic divergence among strains, which could be classified in three different groups: Genotype 1 comprises Movar-like strains (European prototype), Genotype 2 includes DN599-like strains (American prototype) and Genotype 3 corresponds to a novel genotype group. Understanding the replication behavior in cell cultures and the molecular characteristics of this pathogen of cattle is critical for the rational design of in vitro experiments. The aim of this work was to quantitatively evaluate the replication properties of different Argentinean BoHV-4 strains and to characterize their phylogenetic relationships. Significant differences were evident among the virus titers of the different BoHV-4 isolates in vitro. The most conserved gene was the major capsid protein (ORF25). The glycoprotein B (gB), glycoprotein H (gH), and thymidine kinsase (TK) genes displayed both synonymous and non-synonymous substitutions, with the highest diversity observed for gB, which displayed amino acid substitutions in 24 out of the 178 positions examined. Strains 09\/759, 12\/512, and 07\/568 presented a deletion encompassing amino acid position 27 to 35, whereas strains 07\/435 and 09\/227 had a deletion from position 28 to 35. Two strains, 07\/435 and 09\/227, also displayed the highest divergence compared to the other strains analyzed. This study provides information about the in vitro replication and behavior of nine field isolates of BoHV-4. These findings are relevant since available information on the in vitro growth characteristics of BoHV-4 strains is scarce. The results from this study may also be useful for establishing comparisons with other related viruses.","palabras_clave":"Gammaherpesvirus|In vitro replication|Genome variability|Argentinean field strains"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5146,"idioma_id":4,"titulo":"Involvement of toll-like receptors 3 and 7\/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5","resumen":"Bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) are closely related alpha-herpesviruses. BoHV-5 is the causal agent of non-suppurative meningoencephalitis in calves. BoHV-1 causes respiratory disease, abortions, genital disorders and, occasionally, encephalitis in cattle. Both viruses are neurotropic and they share similar biological properties. Nevertheless, they differ in their ability to cause neurological disease. Toll-like receptors (TLRs) are involved in the innate immune response to pathogens. In this study, the variations in the expression levels of TLRs were evaluated in different regions of the bovine central nervous system during the acute infection and reactivation of BoHV-1 and BoHV-5- infected cattle. With the exception of TLR9, significant up-regulation of all TLRs was detected following primary infection of neural tissues by both bovine alpha-herpesviruses. Furthermore, the stages of acute infection and reactivation were characterized by a distinguishable TLR expression pattern. Important differences in TLR expression upon infection of the central nervous system by BoHV-1 or BoHV-5 were not detected. The striking differences in TLR mRNA levels during acute infection and reactivation provide evidence that the innate immune response may be involved in the clinical outcomes observed at each stage. Further research is required to analyze the mechanisms that initiate TLR activation and the signaling cascade mediated by each TLR to elucidate the precise role these receptors play in bovine herpesvirus encephalitis.","palabras_clave":"Bovine herpesvirus|Toll like receptors|Neuropathology|Innate immunity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5147,"idioma_id":5,"titulo":"Método para la cría en laboratorio de Chironomidae (Diptera) de ambientes lóticos","resumen":"La taxonomía de Chironomidae se apoya fuertemente en la integración de las características morfológicas de todas las fases del ciclode vida, las cuales se pueden obtener mediante la cría en laboratorio. Este estudio desarrolló un método de cría en laboratorio delarvas de Chironomidae de ambientes lóticos. El método consta de una circulación constante de agua y distintos ítems alimenticios.Esta metodología ha permitido obtener asociaciones de los estados de larva, pupa y adulto para la identificación de especiespresentes en una región de Argentina en la que el estado de conocimiento de la familia Chironomidae es incipiente.","palabras_clave":"Arroyos|Insectos acuaticos|Ritron"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5148,"idioma_id":4,"titulo":"Holocene compression in the Acequion valley (Andes Precordillera, San Juan province, Argentina): Geomorphic, tectonic, and paleoseismic evidence","resumen":"The Matagusanos-Maradona-Acequion Valley sits within the Andes Precordillera fold-thrust belt ofwestern Argentina. It is an elongated topographic depression bounded by the roughly NeS trendingPrecordillera Central and Oriental in the San Juan Province. Moreover, it is not a piggy-back basin as wecould have expected between two ranges belonging to a fold-thrust belt, but a very active tectoniccorridor coinciding with a thick-skinned triangular zone, squeezed between two different tectonic domains.The two domains converge, where the Precordillera Oriental has been incorporated to the SierrasPampeanas province, becoming the western leading edge of the west-verging broken foreland SierrasPampeanas domain. This latter province has been in turn incorporated into the active deformationframework of the Andes back-arc at these latitudes as a result of enhanced coupling between theconverging plates due to the subduction of the Juan Fernandez ridge that flattens the Nazca slab underthe South American continent.This study focuses on the neotectonics of the southern tip of this NeS elongated depression, known asAcequion (from the homonym river that crosses the area), between the Del Agua and Los Pozos rivers.This depression dies out against the transversely oriented Precordillera Sur, which exhibits a similartectonic style as Precordillera Occidental and Central (east-verging fold-thrust belt). This contributionbrings supporting evidence of the ongoing deformation during the Late Pleistocene and Holocene of thetriangular zone bounded between the two leading and converging edges of Precordillera Central andOriental thrust fronts, recorded in a multi-episodic lake sequence of the Acequion and Nikes rivers. Theherein gathered evidence comprise Late PleistoceneeHolocene landforms of active thrusting, fault kinematics(micro-tectonic) data and outcrop-scale (meso-tectonic) faulting and folding of recent lake andalluvial sequences. In addition, seismically-induced effects already reported in the literature by thisworking team further support the tectonic activity of neighboring faults in the Holocene. As a concludingremark we could state that the ongoing deformation in the region under study is driven by acompressional regime whose maximum horizontal stress in the late PleistoceneeHolocene is roughlyeast-west oriented. This is further supported by focal mechanism solutions.","palabras_clave":"NEOTECTONICS|SEISMOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5149,"idioma_id":4,"titulo":"Seismically-induced soft-sediment deformation structures associated with the Magallanes-Fagnano Fault System (Isla Grande de Tierra del Fuego, Argentina)","resumen":"In this paper, evidence of paleoearthquake-induced soft-sediment deformation structures associated with the Magallanes?Fagnano Fault Systemin the Isla Grande de Tierra del Fuego, southern Argentina, has been identified.Well-preserved soft-sediment deformation structures were found in a Holocene sequence of the Udaeta pond.These structures were analyzed in terms of their geometrical characteristics, deformation mechanism, driving force system and possible trigger agent. They were also grouped in different morphological types: sand dykes,convolute lamination, load structures and faulted soft-sediment deformation features. Udaeta, a small pond in Argentina Tierra del Fuego, is considered a Quaternary pull-apart basin related to the Magallanes?FagnanoFault System. The recognition of these seismically-induced features is an essential tool for paleoseismic studies.Since the three main urban centers in the Tierra del Fuego province of Argentina (Ushuaia, Río Grande and Tolhuin) have undergone an explosive growth in recent years, the results of this study will hopefully contribute to future analyses of the seismic risk of the region.","palabras_clave":"SOFT SEDIMENT STRUCTURE|PALEOEARTHQUAKE|UDAETA POND|FAGNANO LAKE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5151,"idioma_id":4,"titulo":"Fourteen-year status report of fatal illnesses in captive chinchilla (Chinchilla lanigera)","resumen":"Fur ranching has been a prosperous industry since decades. Despite its worldwide distribution, little published information is available regarding the relative or absolute importance of the various causes of death in chinchilla (Chinchilla lanigera). In the period 1999 to 2013, 698 captive chinchillas coming from different commercial ranches were presented for necropsy. Two-hundred and forty-four animals (34.9%) had classical enteritis, 198 (28.4%) had pneumonia, 63 (9.0%) had other infections, 40 (5.8%) had traumatic injuries, 109 (15.6%) had miscellaneous conditions, meanwhile 44 (6.3%) had no significant lesions. Although some disease processes may be underrepresented (i.e., heat stroke and the shock syndrome), the data were collected from a field wide enough and over a sufficient period to give a reliable overview of the fatal problems of this rodent in captivity.","palabras_clave":"Causes of death|Chinchilla lanigera|Fur ranching industry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5152,"idioma_id":4,"titulo":"On the complexity of the {k}-packing function problem","resumen":"Given a positive integer $k$, the ${k}$-emph{packing function problem} (${k}$PF)  is to find in a given graph $G$, a function  $f$ of maximum weight that assigns a non-negative integer to the vertices of $G$ in such a way that the sum of $f(v)$ over each closedneighborhood is at most $k$.  It is known that ${k}$PF is linear time solvable in strongly chordal graphs and in  graphs with clique-width bounded by a constant. Nevertheless, up to now the complexity of ${k}$PF for a general graph is not known. In this paper we prove that ${k}$PF is NP-complete, even when restricted to chordal graphs which constitute a superclass of strongly chordal graphs.Looking for other subclasses of chordal graphs where ${k}$PF is tractable, we prove that it is linear time solvable for doubly chordal graphs, by proving that it is so in the superclass of dually chordal graphs, which are graphs that have a maximum neighborhood ordering.","palabras_clave":"CHORDAL GRAPH|NP-COMPLETENESS|POLYNOMIAL INSTANCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5153,"idioma_id":4,"titulo":"Role of Thioredoxin-1 in ischemic preconditioning, postconditioning and aged ischemic hearts","resumen":"ABSTRACTThioredoxin (Trx)  is one of the most important cellular antioxidant systems known to date, and is responsible of maintaining the reduced state of the intracellular space. Trx-1 is a small cytosolic protein whose transcription is induced by stress. Therefore it is possible that this antioxidant plays a protective role against the oxidative stress caused by an increase of reactive oxygen species concentration, as occurs during the reperfusion after an ischemic episode. However, in addition to its antioxidant properties, it is able to activate other cytoplasmic and nuclear mediators that confer cardioprotection. It is remarkable that Trx-1 also participates in myocardial protection mechanisms such as ischemic preconditioning and postconditioning, activating proteins related to cellular survival. However, comorbidities such as aging can modify this powerful cellular defense, leading to decrease cardioprotection, and even ischemic preconditioning and postconditioning induced in aged animal models failed to decrease infarct size. Therefore, the lack of success of antioxidants therapies to treat ischemic heart disease could be solved, at least in part, avoiding the damage of Trx system","palabras_clave":"PRECONDITIONING|POSTCONDITIONIG|ISCHEMIA|AGING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5154,"idioma_id":4,"titulo":"Thioredoxin-1 attenuates ventricular and mitochondrial post-ischemic dysfunction in the stunned myocardium of transgenic mice","resumen":"ABSTRACTAim: We evaluated the effect of Trx-1 system on post-ischemic ventricular and mitochondrial dysfunction using transgenic mice overexpressing cardiac Trx-1 and a dominant negative (DN-Trx-1) mutant (C32S\/C35S) of Trx-1. Langendorff-perfused hearts were subjected to 15 min of ischemia followed by 30 min of reperfusion (R). We measured left ventricular developed pressure (LVDP, mmHg), end diastolic pressure (LVEDP) and t63 (relaxation index). Mitochondrial respiration, SERCA2a, phospholamban (PLB), and p-PLB (Thr 17) expression (Western blot) were also evaluated. Results: At 30 min of reperfusion Trx-1 improved contractile state (LVDP: Trx-1: 57.4±4.9 vs. Wt: 27.1±6.3 and DN-Trx-1: 29.2±7.1 mmHg, p <0.05); decreased myocardial stiffness (LVEDP: Wt: 24.5±4.8 vs. Trx-1: 11.8±2.9 mmHg, p<0.05) and improved the isovolumic relaxation (t63: Wt: 63.3±3.2 vs. Trx-1: 51.4±1.9, p<0.05). DN-Trx-1 mice aggravated the myocardial stiffness and isovolumic relaxation. Only the expression of p-PLB Thr17 increased at 1.5 min R in Wt and DN-Trx-1 groups. At 30 min of reperfusion state 3 mitochondrial O2 consumption was impaired by 13%) in Wt and by 33% in DN-Trx-1. ADP\/O ratios for Wt and DN-Trx-1 decrease 25% and 28% respectively; whereas the Trx-1 does not change after I\/R. Interestingly, baseline values of complex I activity were increased in Trx-1 mice, they resulted 24% and 47% higher than in Wt and DN-Trx-1 mice, respectively (p<0.01). Innovation and Conclusion: These results strongly suggest that Trx-1 ameliorates the myocardial effects of I\/R by improving the free radical mediated damage in cardiac and mitochondrial function, opening the possibility of new therapeutic strategies in coronary artery disease.","palabras_clave":"Isquemia|Miocardio|Tioredoxina|Ratones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5155,"idioma_id":4,"titulo":"Kinetic study of regional agro-industrial wastes pyrolysis using non-isothermal tga analysis","resumen":"Pyrolysis characteristics and kinetic study of six regional lignocellulosic wastes (sawdust, marcs, stalks, peach plum and olive pits) at different heating rates was investigated using thermogravimetric analysis. The pyrolysis could be divided into three stages, drying, active and passive pyrolysis. The speci&#64257;c location where the thermal degradation reaction are occurring is called active pyrolysis zone. The samples have the highest residual weight equal to 35?40% at 603 K, approximately during this stage.To describe the active pyrolysis behavior, the Coats Redfern and Sharp methods were used. The con-traction geometry?s model describe this phenomenon, indicating that the degradation rate is controlled by the resulting reaction interface progress toward the center of the solid.The activation energy values for the active pyrolysis stage are between 38.96 and 68.99 kJ\/mol for all studied agro-industrial wastes. This parameter increases with the heating rate. This variation can be due to the kinetic rate of active pyrolysis is controlled by the occurrence of physical transformation the temperature-dependent which is not mass dependent. The calculated values of pre-exponential factor were about 2.01 &#8260; 107 and 2.51 &#8260; 1010 s&#1048577;1. These parameters indicate that the pyrolysis of the studied wastes similar to other lignocellulosic materials, proving the feasibility of their valorization by pyrolysis.","palabras_clave":"thermogravimetry analysis|agro-industrial wastes|inert atmosphere|kinetic models"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5157,"idioma_id":4,"titulo":"The impact of moisture sorption properties on the color and bioactives concentrations of black currant-yerba mate instant drinks","resumen":"BACKGROUND: Black currant (BC)and yerba mate (YM) have high contents of polyphenolic antioxidants beneficial for health.Obtaining freeze-dried BC\/YM instant drinks can be a means for providing their advantages to consumers. However, their high sugar contentsmake them very hygroscopic causing undesirable changes in color and bioactives concentration.OBJECTIVE:To solve this problem it is necessary to determine the powder´s sorption properties and the temperature\/relative humidity?s(RH) influence on their color and polyphenol, ascorbic acid and anthocyanins´s concentrations. METHODS:We analyzed the sorption isotherms of freeze-dried YMI\/BC\/maltodextrin\/sugar powder at 10\/20\/40ºC and compared them with those from YM\/maltodextrin.RESULTS: Of all models tested (Caurie, GAB, Halsey, Oswin) GAB was the best. Monolayer moisture values (Wm)were&#8804;0.1 kg H2O(kg d.m)-1 indicating good stability. Due to its higher sugar content, BC\/YM powders were more hygroscopic and with higher exothermic isosteric sorption heat (Qst) than YM powders. Qst and differential entropy decreased exponentially with increasing moisture levels. Within the experimental conditions, isokinetic theory indicated that the whole sorption process was enthalpy controlled. Temperature and RH strongly modified BC\/YM´s color and ascorbic acid and monomeric anthocyanins concentrations. At all temperatures, optimum levels of these properties required RH&#8804;33%. To achieve maximum physicochemical quality and stability the powder?s moisture content must be&#8804;Wm, in this case, RH dropped to 9% (10°C) and 11.3% (20°C\/40°C).","palabras_clave":"BLACK CURRANT|YERBA MATE|SORPTION PROPERTIES|ANTHOCYANINS |ASCORBIC ACID|COLOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5158,"idioma_id":4,"titulo":"Effects of salicylic acid-induced wine rich in anthocyanins on metabolic parameters and adipose insulin signaling in high-fructose fed rats","resumen":"We evaluated the effects of Syrah red wine treated with salicylic acid (RW SA) and its control red wine (RW) on metabolic parameters, systolic blood pressure and adipose tissue insulin signaling in high-fructose (F) fed rats. Grape treated with SA increased the anthocyanin (ANTs) levels in RW. F induced increased systolic blood pressure, dislipidemia and insulin resistance (HOMA:IR). F rats treated with RW significantly prevented these alterations while RW SA partially attenuated triglycerides levels and HOMA:IR without modifications in HDL cholesterol levels. F impaired the adipose tissue response to insulin. Supplementation with RW and RW SA partially attenuated these alterations. Rats supplemented with RW SA had lesser beneficial effects on metabolic alterations than control RW, while both RW and RW SA attenuated altered adipose response to insulin. More studies are necessary to deeply evaluate the effect on SA-induced RW rich in ANTs levels on metabolic alterations associated to MetS.","palabras_clave":"Salicylic acid|red wine anthocyanins|adipose tissue|high-fructose diet"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5159,"idioma_id":4,"titulo":"Ecotoxicological evaluation of foundry sands and cosmetic sludges using new earthworm biomarkers","resumen":"The management and final disposal of industrialwastes are a matter of considerable human concern. Thepresent study evaluates the cyto\/genotoxic effects andchanges of the coelomic cell formulas exerted by aqueousleachates and solid waste (SW) of two industrial residuesusing coelomocytes extruded from Eisenia fetida. Theassayed wastes corresponded to industrial foundry and cosmeticactivities. After 14 days of exposure, we obtained agroup of endpoints that reflect the toxicity\/genotoxicity,coelomocyte formula and indexes; and the mortality classicalvalue (LC50-14d). Among the variables measured, totalcoelomocytes formula (eleocytes ? amebocytes ? granulocytes)appears as a single and easy parameter to assess thetoxicity of eluates at short exposure times. We applied a setof assays using earthworms as test organism that wouldallow evaluating SW as well as its aqueous leachates. It iseasy to run trials combining exposures of 1 h to 14 days,which can be integrated into the implementation of thetraditional test for evaluating acute toxicity","palabras_clave":"GENOTOXICITY|EARTHWORM|HAZARDOUS WASTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5160,"idioma_id":4,"titulo":"Study of Cu-Zn and Au\/TiO2 Catalysts on Anodized Aluminum Monoliths for Hydrogen Generation and Purification","resumen":"This work reports the preparation of Cu-Zn and Au\/TiO2 catalysts on anodized aluminum monoliths (AAM). The structured catalysts were studied for the generation of H2 by methanol steam reforming (MSR) and its purification by preferential oxidation of CO (CO-PrOx). Initially, it was possible to generate a surface with whiskers and larger surface area by hydrothermal treatment of the AAM. Subsequently, the structured catalysts were synthesized by incipient wetness impregnation (IWI) and hydrothermal synthesis (HS). IWI synthesis allowed for the deposition of a larger amount of catalytic material than HS, with very good adhesion. The TiO2-IWI structured catalyst presented a homogeneous catalytic coating, with the presence of agglomerated particles. On the other hand, Cu-Zn-IWI showed good dispersion of the deposited particles with a homogeneous surface coating. EDX analysis corroborated the presence of Ti, Cu and Zn in all the catalytic surfaces. The incorporation of Au over TiO2-IWI structured catalysts was successfully performed by IWI using a colloidal solution of gold nanoparticles. MSR was studied over the developed metallic monoliths functionalized with Cu-Zn by the IWI method. The samples showed promising results in terms of activity, selectivity, and stability. Both diluted and concentrated methanol&#8201;+&#8201;water feeds were assayed. Complete methanol conversion was achieved for the diluted feed. Maximum methanol conversions of 55&#8198;% with 60&#8198;% H2 yield were measured when the concentrated feed was selected. Promising results were also achieved for the Au-based structured catalysts in the CO-PrOx in an H2-rich atmosphere. Although CO conversions of approximately 60&#8198;% were achieved, operating with higher catalyst loadings would be recommended to reach the high CO conversions required for PrOx catalysts.","palabras_clave":"Structured catalysts|Anodized aluminum|Gold|Copper|Zinc"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5161,"idioma_id":4,"titulo":"Discovery of novel polyamine analogs with anti-protozoal activity by computer guided drug repositioning","resumen":"Chagas disease is a parasitic infection caused by the protozoa Trypanosoma cruzi that affects about 6 million people in Latin America. Despite its sanitary importance, there are currently only two drugs available for treatment: benznidazole and nifurtimox, both exhibiting serious adverse effects and limited efficacy in the chronic stage of the disease. Polyamines are ubiquitous to all living organisms where they participate in multiple basic functions such as biosynthesis of nucleic acids and proteins, proliferation and cell differentiation. T. cruzi is auxotroph for polyamines, which are taken up from the extracellular medium by efficient transporters and, to a large extent, incorporated into trypanothione (bis-glutathionylspermidine), the major redox cosubstrate of trypanosomatids. From a 268-compound database containing polyamine analogs with and without inhibitory effect on T. cruzi we have inferred classificatory models that were later applied in a virtual screening campaign to identify anti-trypanosomal compounds among drugs already used for other therapeutic indications (i.e. computer-guided drug repositioning) compiled in the DrugBank and Sweetlead databases. Five of the candidates identified with this strategy were evaluated in cellular models from different pathogenic trypanosomatids (T. cruzi wt, T. cruzi PAT12, T. brucei and Leishmania infantum), and in vitro models of aminoacid\/polyamine transport assays and trypanothione synthetase inhibition assay. Triclabendazole, sertaconazole and paroxetine displayed inhibitory effects on the proliferation of T. cruzi (epimastigotes) and the uptake of putrescine by the parasite. They also interfered with the uptake of others aminoacids and the proliferation of infective T. brucei and L. infantum (promastigotes). Trypanothione synthetase was ruled out as molecular target for the anti-parasitic activity of these compounds.","palabras_clave":"CHAGAS DISEASE|DRUG REPOSITIONING|PAROXETINE|POLYAMINES|TRICLABENDAZOLE|TRYPANOSOMATIDS VIRTUAL SCREENING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5162,"idioma_id":5,"titulo":"Relación entre tratamiento con hormona de crecimiento y perfiles de personalidad en mujeres con diagnóstico de Síndrome de Turner","resumen":"El objetivo del presente trabajo fue identificar la existencia de perfiles distintivos en las escalas de personalidad en relación con la aplicación o no de hormona de crecimiento en 73 mujeres adultas con diagnóstico de Síndrome de Turner de Latinoamérica. Para ello se administró, en formato online, el Inventario Clínico Multiaxial de Millon II y un cuestionario clínico para recabar datos sobre la realización o no de tratamiento con hormona de crecimiento durante el desarrollo con el fin de comparar ambos grupos. Los resultados encontrados muestran que el grupo que no realizó tratamiento con hormona de crecimiento obtiene medias más altas en casi la totalidad de las escalas de personalidad básicas y patológicas en comparación con el grupo que si realizó tratamiento, siendo dicha diferencia significativa en las escalas esquizoide y esquizotípica. Estas escalas se asocian con necesidades afectivas mínimas, aislamiento social y apego empobrecido. Estos resultados podrían dar cuenta que el tratamiento con hormona de crecimiento es un factor positivo para esta población, favoreciendo el desarrollo de características de personalidad más adaptativas en relación a lo social.","palabras_clave":"HORMONA DE CRECIMIENTO|PERSONALIDAD|SÍNDROME DE TURNER|MCMI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5164,"idioma_id":4,"titulo":"Coupled mechanical and fluid flow analysis in fractured saturated porous media using the XFEM","resumen":"In this paper, we study the fluid flow in a deformable porous linear elastic media with a single crack $Gamma$. Fluid exchange between the crack and the surrounding porous media is taken into account through the definition of appropriate boundary conditions on $Gamma$ obtained by applying an averaging process of the Darcy flow within the crack. Two models are considered and compared: a semianalytical one which solves the general potential solution of the singular integral equation modelling the steady state flow in an infinite porous media with one linear crack, obtained by applying the complex potential method, and a numerical one based on the Extended Finite Element Method (XFEM) of the governing equations. The XFEM we apply employs the standard enriched basis functions represented by the Heaviside function on $Gamma$ to describe the discontinuity jump of the displacement field across the crack, the distance function to $Gamma$ to describe the non differentiability of the pressure field across $Gamma$ and the singular functions describing the $sqrt{r}$-singularity at the crack tip of the stress and pressure field, where $r$ is the distance to the crack tip. The semianalytical model is used to verify the application of the XFEM. We include then the coupling with the mechanical response of the body, which is analyzed by using only the XFEM. Several numerical experiments are then carried out which illustrate the variation of the hydro-mechanical quantities around the crack and within the crack.","palabras_clave":"FRACTURED POROUS MEDIA|BIOT-COUSSY MODEL|SINGULAR INTEGRAL EQUATION|EXTENDED FINITE ELEMENT METHOD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5165,"idioma_id":4,"titulo":"Effects  of   Pb  in Tagetes minuta L. (Asteraceae) leaves and its relationship  with volatile  compounds","resumen":"traditionally been the disposal site for most heavy metal waste. Since metals can not be degraded,soil remediation requires their removal with phytoextraction, being a cost effective alternative in whichmetal accumulator plants are used to remove toxic metals from soil. Aromatic crops, used for the productionof essential oils, may be a suitable alternative to be used in agricultural soils contaminated withheavy metals, since after removal, a marketable product is left. The present study investigated the effectsthat high concentrations of lead (Pb) have on the volatile compounds in leaves of Tagetes minuta L., growingnear a battery recycling plant and also determined whether Pb was present in its essential oil. Inthis way, it could be evaluated whether the use of this species for Pb phytoremediation supports safeproduction. To carry this out, it was determined whether the essential oil obtained by hydrodistillationcontained Pb, which was also checked for each individual of T. minuta, by measuring the amount of thismetal in leaves using the technique of X-ray fluorescence and calculating the percentages of volatiles bythe HS-SPME technique.The essential oils extracted by hydrodistillation did not have any detectable Pb, there by demonstratingthat it is not transferred to the essential oil. Regarding the volatiles, the highest concentrations encounteredwere for cis-tagetone, dihydrotagetone and verberone. Furthermore, the compounds -ocimeneand -thujone correlated significantly with the Pb concentration in leaves, indicating that the increaseof Pb in T. minuta may favor synthesis of these compounds. In conclusion, T. minuta is a resistant plantthat can grow in Pb polluted soils and accumulate the contaminant in aerial tissues, while providing aneconomic return through the production of essential oil free of Pb","palabras_clave":"TAGETES MINUTA L.|LEAD|PHYTOEXTRACTION|VOLATILE COMPOUNDS|ESSENTIAL OIL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5166,"idioma_id":4,"titulo":"MANDARIN ESSENTIAL OILS INHIBIT QUORUM SENSING AND VIRULENCE FACTORS OF PSEUDOMONAS AERUGINOSA","resumen":"Pseudomonas aeruginosa produces biofilm and several virulence factors coordinated by quorum sensing (QS) in food. The interruption of QS is a target to control the bacterial virulence. Chemical preservatives used to control biofilm give rise to several food safety problems. For this reason, essential oils (EOs),generally recognized as safe products, are a hopeful alternative. The aim of this work was to determine the chemical composition of mandarin EOs obtained by cold-pressing (EOP) and cold-pressing followed by steam distillation (EOPD) and their antipathogenic properties against P. aeruginosa. Both EOs containedthe highest quantities of monoterpene hydrocarbons, mainly limonene, followed by g-terpinene, myrcene and a-pinene. Although the EOs were not able to inhibit the bacterial growth at 4 mg\/mL, EOP and EOPD significantly inhibited the P. aeruginosa biofilm formation at 0.1 mg\/mL. In addition to the EOsinhibited biofilm cell viability (41%), AHL production (33%) and resulted in higher than 75% reduction in elastase enzyme activity. In conclusion, Citrus reticulata EOs are suitable alternatives to chemical additives for general use in the food industry.","palabras_clave":"Citrus reticulata|ESSENTIAL OILS|BIOFILMS|Pseudomonas aeruginosa |ANTIPATHOGENIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5167,"idioma_id":4,"titulo":"Inhibition kinetics during the oxidation of binary mixtures of phenol with catechol, resorcinol and hydroquinone by phenol acclimated activated sludge","resumen":"In this work the aerobic degradation of phenol (PH), catechol (CA),resorcinol (RE), hydroquinone (HY) and the binary mixtures PH+CA, PH+RE, PH+HYby phenol-acclimated activated sludge was studied. Single substrate experimentsshow a Haldane-type dependence of the respiration rate on PH, RE and HY whilethe Monod model corresponded to CA. Binary substrate experiments demonstratedthat the presence of a second substrate only affected the kinetics, but not thestoichiometry of the oxidation of the tested compounds. While CA inhibited theoxidation of PH, PH inhibited the oxidation of RE and HY. A mathematical modelwas developed to represent the aerobic biodegradation of the tested phenoliccompounds. The agreement between the proposed model and the experimental data indicatesthat the proposed model can be useful for predicting substrates and dissolvedoxygen concentrations in bioreactors treating phenolic wastewaters. ","palabras_clave":"Phenolic compounds|Biodegradation|Respirometry|Activated sludge|Mathematical model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5168,"idioma_id":5,"titulo":"REALIDAD DE LA RESPONSABILIDAD SOCIAL DE LAS ORGANIZACIONES (RSO) EN VILLA MARÍA","resumen":"La responsabilidad social es una realidad mundial que se materializa día tras día a partir del accionar humano, pero ¿cómo se expande a toda la sociedad? Conscientes de que el motor de la sociedad son las organizaciones, el desafío de las personas consiste en transformarlas en agentes socialmente responsables e iniciar de esta manera el círculo virtuoso de ?ganar-ganar? con sustentabilidad.Una conducta organizacional responsable amerita el análisis de diferentes aspectos de la gestión organizacional, y no tan sólo de la mera obligación, ni de causas filantrópicas. Una gestión en materia de responsabilidad social, significa ampliar la mirada hacia los impactos que genera laorganización, y a través de los mismos, ir considerando acciones que puedan disminuir la incidencia en los impactos negativos que hubiera, y además planificar acciones tendiente a un desarrollo social y ambiental.Como habitantes de Villa María, provincia de Córdoba, nos ha interesado conocer cómo se encuentran las organizaciones de Villa María en materia de Responsabilidad Social.","palabras_clave":"RESPONSABILIDAD|SOCIAL|FILANTROPIA|RESPONSABILIDAD ORGANIZACIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5169,"idioma_id":4,"titulo":"Uncontrolled Citrus psorosis virus infection in Citrus sinensis transgenic plants expressing a viral 24K-derived hairpin that does not trigger RNA silencing","resumen":"Citrus psorosis virus (CPsV) is the causal agent of psorosis disease of citrus. Pineapple sweet orange plants were transformed with a hairpin construct derived from the viral 24k gene (lines ihp24K). Contrary to expectations, these lines did not trigger efficient RNA silencing, and when infected with CPsV they showed a phenotype of exacerbated symptoms with a persistent and homogeneous infection without the recovery observed in non-transgenic plants. Ihp24K lines did not behave similarly when challenged with Citrus tristeza virus. All these results indicate that hypersusceptibility is likely related to the specific action of 24K-derived hairpin over CPsV multiplication.","palabras_clave":"CITRUS PSOROSIS VIRUS|HAIRPIN-RNA|TRANSGENIC SWEET ORANGE|HYPERSUSCEPTIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5170,"idioma_id":4,"titulo":"Coarse grained study of pluronic F127: Comparison with shorter co-polymers in its interaction with lipid bilayers and self-aggregation in water","resumen":"The aim of this work is to understand the interactions of the poloxamer Pluronic F127, with lipid bilayersand its ability to self-associate in an aqueous environment. Molecular dynamics simulations at thecoarse-grain scale were performed to address the behavior of single Pluronic F127 and shorter poloxamersunimers in palmitoyl-oleoyl-phosphatidyl-choline model membranes. According to the initialconditions and the poly-ethylene oxide\/poly-propylene oxide composition, in water phase the unimerchain collapses into a coil conformation or adopts an interphacial U-shaped e or membrane spanning edistribution. A combination of poly-propylene oxide length, and the poly-ethylene oxide ability to coverpoly-propylene oxide, is determinant for the conformation adopted by the unimer in each phase. Resultsof the simulations showed molecular evidence of strong interaction between Pluronic F127 and modelmembranes both in stable U-shaped and span conformations. The knowledge of this interaction couldcontribute to improve drug permeation. Additionally, we investigated the aggregation of one hundredPluronic F127 unimers in water forming a micelle-like structure, suitable to be used as drug deliverysystem models.","palabras_clave":"POLOXAMERS|MOLECULAR DYNAMICS|LIPID BILAYERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5171,"idioma_id":4,"titulo":"Second-order magnetic critical points at finite magnetic fields: Revisiting Arrott plots","resumen":"The so-called Arrott plot, which consists in plotting H\/M against M2, with H the applied magnetic field and M the magnetization, is used to extract valuable information in second-order magnetic phase transitions. Besides, it is widely accepted that a negative slope in the Arrott plot is indicative of a first-order magnetic transition. This is known as the Banerjee criterion. In consequence, the zero-field transition temperature T* is reported as the characteristic first-order transition temperature. By carefully analyzing the mean?field Landau model used for studying first-order magnetic transitions, we show in this work that T* corresponds in fact to a triple point where three first-order lines meet. More importantly, this analysis reveals the existence of two symmetrical second-order critical points at finite magnetic field (Tc,±Hc). We then show that a modified Arrott plot can be used to obtain information about these second-order critical points. To support this idea we analyze experimental data on La2\/3Ca1\/3MnO3 and discuss an estimate for the location of the triple point and the second-order critical points.","palabras_clave":"ARROTT PLOT|PHASE TRANSITION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5172,"idioma_id":4,"titulo":"A quantum-mechanics molecular-mechanics  scheme for extended systems","resumen":"We introduce and discuss a hybrid quantum-mechanics molecular-mechanics (QM-MM)approach for Car?Parrinello DFT simulations with pseudopotentials and planewaves basis,designed for the treatment of periodic systems. In this implementation the MM atoms areconsidered as additional QM ions having fractional charges of either sign, which providesconceptual and computational simplicity by exploiting the machinery already existing inplanewave codes to deal with electrostatics in periodic boundary conditions. With this strategy,both the QM and MM regions are contained in the same supercell, which determines theperiodicity for the whole system. Thus, while this method is not meant to compete with non-periodic QM-MM schemes able to handle extremely large but finite MM regions, it is shownthat for periodic systems of a few hundred atoms, our approach provides substantial savingsin computational times by treating classically a fraction of the particles. The performance andaccuracy of the method is assessed through the study of energetic, structural, and dynamicalaspects of the water dimer and of the aqueous bulk phase. Finally, the QM-MM scheme isapplied to the computation of the vibrational spectra of water layers adsorbed at the TiO2anatase (1 0 1) solid?liquid interface. This investigation suggests that the inclusion of a secondmonolayer of H2O molecules is sufficient to induce on the first adsorbed layer, a vibrationaldynamics similar to that taking place in the presence of an aqueous environment. The presentQM-MM scheme appears as a very interesting tool to efficiently perform molecular dynamicssimulations of complex condensed matter systems, from solutions to nanoconfined fluids todifferent kind of interfaces.","palabras_clave":"QM-MM|molecular dynamics|DFT|interfaces"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5173,"idioma_id":4,"titulo":"Epipelon dynamics in a shallow lake through a turbid-and a clear- water regime","resumen":"In Pampean lakes, very little information is available on epipelon structure and dynamics or the effect of environmental variables on those parameters. We anticipate that light may have the greatest influence on this community and that the nutrient concentration and substrate instability may exert secondary effects. Our objective was to analyze variations in descriptive parameters and in the algal species composition of the epipelon in the Pampean Lake Lacombe over space and time to establish the most influential environmental forces (light climate, wave action, nutrients). Sediment was collected from the benthos with a 3.5-cm-diameter corer in a year-round sampling every month at four sites in the lake that differed from each other in depth, distance from the shore, and the presence of emergent and submerged macrophytes. Although Lacombe is a shallow lake, differences occurred in community descriptive variables and in the epipelon composition (especially diatoms) over space and time. In particular, differences between the site nearest to the shore and the rest of the sites were found with respect to environmental variables (sediment-particle size, total phosphorus, organic matter content, light climate), epipelon descriptive parameters (abundance, chlorophyll a and Margalef?s index) and epipelon composition (especially specific biovolume and diatom-size classes). The epipelon also reflected, though slightly, the change from the turbid- to clear-water states in the lake. Sediment erosion or deposition was evidenced by differences in the sediment-particle composition and the predominant particle-size classes at each site. Statistical analyses indicated linear correlations between the epipelon parameters and variables related to the light climate in the water column. Conductivity and sediment total phosphorus and density were also correlated, but to a lesser extent, with epipelon composition. The epipelon variations in Lacombe Lake would exemplify the reference communities developed during clear or turbid regimes in Pampean shallow lakes. We found evidence for the influence of light climate and wave action (through instability of the substrate) on this community, but the effect of nutrients was negligible. We emphasize that the close relationships maintained by the epipelon with other communities such as the phytoplankton and the epiphyton may superimpose the patterns of variation to those communities onto the epipelon dynamics.","palabras_clave":"EPIPELON|COMMUNITY  VARIATIONS|PAMPEAN SEEPAGE LAKE|ARGENTINA|SHALLOW LAKE REGIMES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5174,"idioma_id":5,"titulo":"EL PERIFITON COMO INDICADOR DE CONTAMINACIÓN DIFUSA: EL CASO DE LA CUENCA SUPERIOR DEL RÍO RECONQUISTA (BUENOS AIRES, ARGENTINA)","resumen":"Se estudió el perifiton de la cuenca superior del río Reconquista con el objetivo de identificar las especies que lo componen y analizar las posibles modificaciones en su estructura debidas a la contaminación difusa provocada por las actividades agropecuarias. Se realizaron entre diciembre de 2006 y mayo de 2008 seis muestreos a intervalos de tres meses en ocho tramos adyacentes a campos donde se desarrollan actividades agrícolas y ganaderas. Aplicando análisis de similitud entre comunidades se encontraron diferencias significativas entre ambos tipos de actividad siendo más alta la concentración de clorofila-a y menores los índices de autotrofia en zonas agrícolas. Se contabilizaron un total de 158 especies con una riqueza específica muy variable (mínimo 10 especies y máximo 67 especies) por tramo. No se encontró una marcada dominancia por parte de alguna de las especies y ni en la diversidad estimada por el índice de Shannon en todos los sitios. Se concluye en cuanto a la composición específica que la presencia de Ulnaria ulna y Gomphonema parvulum se asocia a sitios agrícolas mientras que Nitzschia palea, Raphidiopsis mediterranea y Trachelomonas sp3 a sitios ganaderos.","palabras_clave":"Perifiton|Contaminación difusa|Río Rconquista|Agricultura |Ganadería"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5175,"idioma_id":4,"titulo":"Geochemistry in Argentina: from        pioneers to the present","resumen":"ArgentineGeochemistry evolved during the 19th century hand in hand with other sciences.The Scotsman John J. Kyle was the first chemist to arrive in Argentina in 1862, contributingduring his lifetime reports that expanded the geochemical knowledge of localnatural resources. After visiting the USAand Europe (1868), Argentina?sPresident Sarmiento requested Hermann Burmeister (a prestigious biologist) toengage European scientists to foster the teaching and research of NaturalSciences (sensu lato) in Argentina.The first to arrive, in August 1871 at the National Academy of Sciences and theuniversity in Córdoba, was Max Siewert, a chemist from the German Martin Luther University.Siewert set up a state-of-the-art laboratory and analysed, as Kyle had a fewyears before, a range of materials from waters and minerals to natural salts andbiological materials. Some years later, Adolf Döring replaced Siewert. In the20th century, Gustavo Fester is the personality to highlight as achemist\/geochemist because he accomplished a vast task as teacher andresearcher at the Universidad Nacional delLitoral and other institutions. During the 1950?s and 1960?s ArgentineGeochemistry experienced slow but sustained growth, promoted by competentuniversity professors such as Félix González Bonorino, José Catoggio, MarioTeruggi and Carlos Gordillo, The first Geochemistry curriculum was initiated in1958 at the Universidad Nacional de LaPlata as a result of the bold initiative of Catoggio and Teruggi. Nowadays,Geochemistry is solidly established in Earth Sciences curricula and Argentinegeochemical papers are found in all the international journals of the specialty.&nbsp;","palabras_clave":"Earth Sciences|Chemistry|Geology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5176,"idioma_id":4,"titulo":"Mafic rocks of the Ordovician Famatinian magmatic arc (NW Argentina): New insights into the mantle contribution","resumen":"We studied the petrogenesis of mafic igneous rocks in the Famatinian arc in the western Sierra Famatina (NW Argentina), an Early Ordovician middle-crustal section in the proto-Andean margin of Gondwana. Mafic rock types consist of amphibolite, metagabbro, and gabbro, as well as pod- and dike-like bodies of gabbro to diorite composition. Field relations together with geochemical and isotopic data for the mafic rocks of the western Sierra de Famatina (at 29&deg;S) define two contrasting suites, which can be correlated with similar assemblages noted in other parts of the orogen. Amphibolite, metagabbro, and gabbro bodies are mostly the oldest intrusive rocks (older than 480 Ma), with the host tonalite and post-tonalite mafic dikes being slightly younger. The older mafic suite is tholeiitic to calc-alkaline and isotopically evolved, except for most of the amphibolite samples. The younger suite is calc-alkaline, typically displaying subduction-related geochemical signatures, and it is isotopically more juvenile. Whole-rock chemical composition and isotopic analyses are compatible with a progressive mixing of different isotopic reservoirs. Pyroxenite (&plusmn;garnet) was likely the dominant source of the older gabbroic magmas, whereas peridotite dominated in the source of the younger suite, implying that the mafic magma experienced a progressive shift toward more juvenile compositions though time (over 20 m.y.). Pyroxenitederived melts could have been generated by lithospheric foundering followed by upwelling of primitive melts by adiabatic decompression of mantle wedge peridotite.","palabras_clave":"PETROGENESIS|MAFIC ROCKS|FAMATINIAN ARC|MANTLE CONTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5177,"idioma_id":4,"titulo":"What drives the use of natural ´ products for medicinal purposes in contexts of cultural pluralism?","resumen":"Introduction: What are the drivers of health seeking behaviors in a culturally diverse scenario? A contextof medical and cultural pluralism with native and migrant people in NE Brazil was chosen to study the useof complementary and alternative medicine (CAM) and official medicine (OM) products and their drivers.Methods: A one-year therapeutic recall was used to obtain information on individual health problems,products to treat their problems and access to health services. A generalized linear model (GLM (binomialerrors) was employed to indicate the variables that better explained therapeutic choices.Results: Most people use both CAM and OM products and most illnesses are treated by both systems. TheGLM indicated that women, migrants and people that turns to primary health-care services (PHCs) in alower extent are more likely to exclusively chose CAM products and are also more likely to prioritizethem. Frequentation of the local health center also leads to a proportionally higher use of CAM productsthan the frequentation of other PHCs from the surroundings.Conclusions: Women, migrants and people that do not frequent PHCs are the main supporters of CAMproducts. In the case of migrant people, their high adhesion to CAM products (especially medicinalplants) can turn them into new depositors of local (traditional) knowledge, although this knowledge willnot be dissoluble from other types of medicinal plant knowledge.","palabras_clave":"complementary and alternative medicine|health seeking behaviour|multiculturalism|traditional medicine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5178,"idioma_id":4,"titulo":"Trypanosoma cruzi polyamine transporter: its role on parasite growth and survival under stress conditions","resumen":"Paper seleccionado y comentado en Atlas of Science (http:\/\/atlasofscience.org\/polyamine-transport-increases-drug-resistance-in-the-parasite-trypanosoma-cruzi\/)Abstract Trypanosoma cruzi is the etiological agent of Chagas disease, a major health problem in Latin America. Polyamines are polycationic compounds that play a critical role as regulators of cell growth and differentiation. In contrast with other protozoa, T. cruzi is auxotrophic for polyamines because of its inability to synthesize putrescine due to the lack of both, arginine and ornithine decarboxylase; therefore, the intracellular availability of polyamines depends exclusively on transport processes. In this work, the polyamine transporter TcPAT12 was over-expressed in T. cruzi epimastigotes demonstrating that growth rates at different concentrations of polyamines strongly depend on the regulation of the polyamine transport. In addition, parasites overexpressing TcPAT12 showed a highly increased resistance to hydrogen peroxide and the trypanocidal drugs nifurtimox and benznidazole, which act by oxidative stress and interfering the synthesis of polyamine derivatives, respectively. Finally, the presence of putative polyamine transporters was analyzed in T. Trypanosoma brucei, and Leishmania major genomes identifying 3?6 genes in these trypanosomatids.","palabras_clave":"TRYPANOSOMA CRUZI|POLYAMINES|PERMEASES|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5179,"idioma_id":4,"titulo":"Neuronal Filopodium Formation Induced by the Membrane Glycoprotein M6a (Gpm6a) is Facilitated by Coronin-1a, Rac1 and p21-Activated Kinase 1 (Pak1).","resumen":"Stress-responsive neuronal membrane glycoprotein M6a (Gpm6a) functions in neurite extension, filopodium and spine formation and synaptogenesis. The mechanisms of Gpm6a action in these processes are incompletely understood. Previously, we identified the actin regulator Coronin-1a (Coro1a) as a putative Gpm6a interacting partner. Here, we used coimmunoprecipitation assays with the anti-Coro1a antibody to show that Coro1a associates with Gpm6a in rat hippocampal neurons. By immunofluorescence microscopy we demonstrated that in hippocampal neurons Coro1a localizes in F-actin enriched regions and some of Coro1a spots colocalize with Gpm6a labeling. Notably, the overexpression of a dominant-negative form of Coro1a as well as its downregulation by siRNA interfered with Gpm6a-induced filopodium formation. Coro1a is known to regulate the plasma membrane translocation and activation of small GTPase Rac1. We show that Coro1a coimmunoprecipitates with Rac1 together with Gpm6a. Pharmacological inhibition of Rac1 resulted in a significant decrease of filopodium formation by Gpm6a. The same was observed upon the coexpression of Gpm6a with the inactive GDP-bound form of Rac1. In this case the elevated membrane recruitment of GDP-bound Rac1was detected as well. Moreover, the kinase activity of the p21-activated kinase 1 (Pak1), a main downstream effector of Rac1 that acts downstream of Coro1a, was required for Gpm6a-induced filopodium formation. Taken together, our results provide evidence that a signaling pathway including Coro1a, Rac1, and Pak1 facilitates Gpm6a-induced filopodium formation.","palabras_clave":"Coronin-1a|Ras-related C3 botulinum toxin substrate 1 (Rac1)|filopodium|hippocampal neuron|membrane glycoprotein M6a|rat"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5180,"idioma_id":4,"titulo":"Noncoding RNAs in smooth muscle cell homeostasis: implications in phenotypic switch and vascular disorders","resumen":"Vascular smooth muscle cells (SMC) are a highly specialized cell type that exhibit extraordinary plasticity in adult animals in response to a number of environmental cues. Upon vascular injury, SMC undergo phenotypic switch from a contractile-differentiated to a proliferative\/migratory-dedifferentiated phenotype. This process plays a major role in vascular lesion formation and during the development of vascular remodeling. Vascular remodeling comprises the accumulation of dedifferentiated SMC in the intima of arteries and is central to a number of vascular diseases such as arteriosclerosis, chronic obstructive pulmonary disease or pulmonary hypertension. Therefore, it is critical to understand the molecular mechanisms that govern SMC phenotype. In the last decade, a number of new classes of noncoding RNAs have been described. These molecules have emerged as key factors controlling tissue homeostasis during physiological and pathological conditions. In this review, we will discuss the role of noncoding RNAs, including microRNAs and long noncoding RNAs, in the regulation of SMC plasticity.","palabras_clave":"SMOOTH MUSCLE CELLS|PHENOTYPIC CHANGE|NONCODING RNA|VASCULAR REMODELING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5181,"idioma_id":4,"titulo":"Synthesis of a Small Library of Imidazolidin-2-ones using Gold Catalysis on Solid Phase","resumen":"An efficient and high-yielding solid phase synthesisof a small library of imidazolidin-2-ones and imidazol-2-ones was carried outemploying a high chemo- and regio-selective gold-catalyzed cycloisomerizationas a key step. Polymer-supported amino acids derivatized with several alkynesfunctionalities combined with tosyl- and phenyl-ureas have been subjected togold-catalysis exhibiting exclusively C?N bond formation. The present workproves the potential of solid phase synthesis and homogeneous gold catalysis asan efficient and powerful synthetic tool for the generation of drug-likeheterocycles.","palabras_clave":"Gold catalysis|Solid-Phase Synthesis|C-N bond formation|Propargylureas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5182,"idioma_id":4,"titulo":"A Spectrophotometric Study of Plumage Color in the Eared Dove (Zenaida auriculata), the Most Abundant South American Columbiforme","resumen":"For birds, plumage color perception is critical in social interactions such as courtship, in both monochromatic and dichromatic species. In the Eared Dove (Zenaida auriculata), perhaps the most abundant South American Columbiforme, the plumage of males and females looks alike and both sexes share the same melanistic coloration with gray and pink tones. The aim of this study was therefore to determine whether evident sexual dichromatism exists in the plumage of the Eared Dove using a spectrophotometry technique in the avian-visible range (300-700 nm). The results of the classic colorimetric variables analysis (hue, chroma and brightness) show that males are in general brighter and have higher UV chroma values than females. The avian visual model points to differences in achromatic and chromatic levels between males and females in body regions possibly involved in sexual selection (e.g. the crown).The model also indicates chromatic or achromatic differences in body regions not subject to sexual selection such as the black spots on the wing coverts and white tail bands, both of which may be involved in intra- or inter-gender-specific communication.","palabras_clave":"ZENAIDA AURICULATA|PLUMAGE COLOR|SEXUAL SELECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5183,"idioma_id":4,"titulo":"Drift effects on the multivariate floral phenotype of Calceolaria polyrhiza during a post-glacial expansion in Patagonia","resumen":"Quaternary environmental changes substantially impacted the landscape and promoted rapid evolutionary changes in many species; however, analyses of adaptive phenotypic variation in plants have usually neglected the underlying historical context. Here we associate phylogeography and phenotypic evolution by analyzing the divergence of Calceolaria polyrhiza multivariate floral phenotype after a Pleistocene postglacial expansion in Patagonia. Phenotypic matrix (P) properties (size, shape, orientation and phenotypic integration) of six refugium and six recent populations from two different phylogroups werecompared following different approaches. We found that P-matrix shape and orientation remained stable despite the strong phylogeographic footprint of postglacial expansion.However, average proportional reductions in matrix size supported the expectation that drift had a significant effect on the floral phenotype in the northern phylogroup. When phylogeographic history was not included in the analyses, results overestimated phenotypic differences, whereas under explicit phylogeographic control, drift appeared as the best explanation for matrix differences. In general, recent populations showed a larger henotypicdivergence among them but a lower overall phenotypic variation than refugium populations. Selection skewers analyses indicated a lower potential response to selection in recently colonized populations than in refugium populations. We discuss that the combination of phylogeographic analyses with geographic distribution of functional phenotypic (genotypic) variation is critical not only to understand how historical effects influence adaptive evolution,but also to improve field comparisons in evolutionary ecology studies.","palabras_clave":"COMMON PRINCIPAL COMPONENTS ANALYSIS|GENETIC DRIFT|PATAGONIA|PHENOTYPIC INTEGRATION|PLEISTOCENE RADIATIONS|P-MATRIX|RANDOM SKEWERS|VARIANCE-COVARIANCE STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5184,"idioma_id":4,"titulo":"New recommendations for measuring collagen solubility","resumen":"The heat-solubility of intramuscular collagen is usually conducted in 1\/4 Ringer´s solution at pH 7.4, despite this ionic strength and pH being inappropriate for post-rigor meat. The current work studied the percentage of soluble collagen and hydrothermal isometric tension characteristics of perimysial strips on bovine semitendinosus muscles in either 1\/4 Ringer´s solution, distilled water, PBS, or a solution of the same salt concentration as 1\/4 Ringer´s but at pH 5.6. Values of % soluble collagen were lower at pH 7.4 than 5.6. Increasing ionic strength reduced % soluble collagen. The maximum perimysial isometric tension was independent of the bathing medium, but the percent relaxationwas higher at pH 7.4 than at pH 5.6, and increasedwith ionic strength of themedia. It is recommended that future measurements of collagen solubility and tests on connective tissue components ofpost-rigor meat should be carried out in a solution of concentrations NaCl and KCl equivalent to those in 1\/4 Ringer´s, but at pH 5.6, a pH relevant to post-rigor meat.","palabras_clave":"colagen solubility|meat"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5185,"idioma_id":4,"titulo":"The herbicide glyphosate is a weak inhibitor of acetylcholinesterase in rats","resumen":"The current work evaluated the inhibitory potency of the herbicide glyphosate (GLP) on acetylcholinesterase (AChE) activity in male and female rat tissues. The AChE activity in brain was higher (p < 0.05)than those observed in kidney (females: 2.2-fold; males: 1.9-fold), liver (females: 6-fold; males:6.9-fold) and plasma (females: 14.7-fold; males: 25.3-fold). Enzyme activities were higher in presence of 10 mM GLP compared to those measured at an equimolar concentration of the potent AChE inhibitor dichlorvos (DDVP). Moreover, IC50s for GLP resulted between 6 × 104- and 6.8 × 105-fold higher than those observed for DDVP. In conclusion, GLP is a weak inhibitor of AChE in rats","palabras_clave":"glyphosate|acetylcholinesterase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5186,"idioma_id":4,"titulo":"Electrical Oscillations in Two-Dimensional Microtubular Structures","resumen":"Microtubules (MTs) are unique components of the cytoskeleton formed by hollow cylindrical structures of &#945;&#946; tubulin dimeric units. The structural wall of the MT is interspersed by nanopores formed by the lateral arrangement of its subunits. MTs are also highly charged polar polyelectrolytes, capable ofamplifying electrical signals. The actual nature of these electrodynamic capabilities remains largely unknown. Herein we applied the patch clamp technique to two-dimensional MT sheets, to characterize their electrical properties. Voltage-clamped MT sheets generated cation-selective oscillatoryelectrical currents whose magnitude depended on both the holding potential, and ionic strength and composition. The oscillations progressed through various modes including single and double periodic regimes and more complex behaviours, being prominent a fundamental frequency at 29 Hz. In physiological K+ (140 mM), oscillations represented in average a 640% change in conductance that was also affected by the prevalent anion. Current injection induced voltage oscillations, thus showing excitability akin with action potentials. The electrical oscillations were entirely blocked by taxol, with pseudo Michaelis-Menten kinetics and a KD of ~1.29 &#956;M. The findings suggest a functional role of the nanopores in the MT wall on the genesis of electrical oscillations that offer new insights into the nonlinear behaviour of the cytoskeleton.","palabras_clave":"Microtubules|Currents|Oscillator|Nanopores|Taxol"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5188,"idioma_id":4,"titulo":"The eye and the skin in endocrine metabolic diseases","resumen":"The eye and skin may offer critical clues to the diagnosis of a varied spectrum of metabolic diseases from endocrine origin and their different stages of severity, such as diabetes mellitus and Graves disease. On the other hand, such entities may compromise the eye and visual function severely, and awareness of these possible associations is an important step in their diagnosis and management. A large number of less common endocrine diseases may also have significant ocular\/visual or skin involvement. Often the etiologic relationship between the endocrine metabolic disease and the ocular compromise is unknown, but diverse pathogenetic mechanisms may act through a common pathologic pathway producing ocular damage, as occur in diabetic retinopathy. This review emphasizes the ocular and skin manifestations of different metabolic diseases of endocrine origi","palabras_clave":"METABOLIC DISEASES|ENDOCRINE DISEASES|EYE|SKIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5189,"idioma_id":4,"titulo":"Vascular Tone Regulation Induced by C-Type Natriuretic Peptide: Differences in Endothelium-Dependent and -Independent Mechanisms Involved in Normotensive and Spontaneously Hypertensive Rats","resumen":"Given that the role of C-type natriuretic peptide (CNP) in the regulation of vascular tone in hypertensive states is unclear, we hypothesized that impaired response of the nitric oxide system to CNP in spontaneously hypertensive rats (SHR) could affect vascular relaxation induced by the peptide in this model of hypertension, and that other endothelial systems or potassium channels opening could also be involved. We examined the effect of CNP on isolated SHR aortas, and the hindlimb vascular resistance (HVR) in response to CNP administration compared to normotensive rats. Aortas were mounted in an isometric organ bath and contracted with phenylephrine. CNP relaxed arteries in a concentration-dependent manner but was less potent in inducing relaxation in SHR. The action of CNP was diminished by removal of the endothelium, inhibition of nitric oxide synthase by N&#969;-nitro-L-arginine methyl ester, and inhibition of soluble guanylyl cyclase by 1H-[1,2,4]oxadiazolo[4,3-alpha]quinoxalin-1-one in both groups. In contrast, blockade of cyclooxygenase or subtype 2 bradykinin receptor increased CNP potency only in SHR. In both Wistar and SHR, CNP relaxation was blunted by tetraethylammonium and partially inhibited by BaCl2 and iberiotoxin, indicating that it was due to opening of the Kir and BKCa channels. However, SHR seem to be more sensitive to Kir channel blockade and less sensitive to BKCa channel blockade than normotensive rats. In addition, CNP decreases HVR in Wistar and SHR, but the effect of CNP increasing blood flow was more marked in SHR. We conclude that CNP induces aorta relaxation by activation of the nitric oxide system and opening of potassium channels, but the response to the peptide is impaired in conductance vessel of hypertensive rats.","palabras_clave":"HYPERTENSION|NATRIURETIC PEPTIDES|ENDOTHELIUM|VASCULAR FUNCTION|NITRIC OXIDE|POTASSIUM CHANNELS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5190,"idioma_id":4,"titulo":"The eye and the skin in nonendocrine metabolic disorders","resumen":"As metabolism is controlled by the input of genes and the environment, metabolic disorders result from some disturbance in the interaction between genes and environmental factors. Many metabolic disorders consist in congenital enzyme deficiencies, also known as 'inborn errors of metabolism,' that may be disabling or cause severe illness and death and are predominantly inherited in an autosomal recessive fashion. The deposit in cells and tissues of storage substances from errors in metabolic processes may produce a wide variety of disorders affecting different organs and functions, with different degrees of severity, and often present around the time of birth or early childhood. Distinctive ocular and skin manifestations accompany many metabolic diseases and may provide clues for their diagnosis and evolution.","palabras_clave":"METABOLIC DISEASES|EYE|SKIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5191,"idioma_id":4,"titulo":"Associations between disease activity markers of HDL functionality and arterial stiffness in patients with rheumatoid arthritis","resumen":"Background and aims: Rheumatoid arthritis (RA) is a chronic, inflammatorydisease associated with increased risk of cardiovascular disease (CVD). Measures of HDL metabolism\/function were shown to be altered in RA patients with high disease activity. We aimed at evaluating the effect of HDL characteristics on arterial stiffness in RA patients classified according to the inflammatory disease activity.Methods: RA patients were classified according to disease activity (DAS-28) into active RA (n=27; DAS-28>3.2) and inactive RA patients (n=17; DAS-28<3.2). A control group of healthy individuals was also studied (n=33). Clinical and biochemical characteristics, cholesteryl ester transfer protein (CETP) andparaoxonase 1 (phenylacetate and paraoxonase) activities and carotid-femoralpulse wave velocity (cf-PWV) were determined.Results: Anthropometric characteristics were similar in all groups. In accordance with the inflammatory status, active RA patients presented elevated hsCRP levels (p <0.001). There were no differences in the lipid profile between groups. Similarly, features of insulin resistance were absent in RA patients (p=non-significant). Active RA patients presented higher CETP activity than the other two groups (p=0.026). Phenylacetate and paraoxonase activities were altered in active RA patients compared with the other groups (p=0.034 and p=0.041, respectively). Cf-PWV was significantly higher in active RA patients in comparison with controls, following adjustment by age (p=0.030). Age (&#946;st=0.468, p=0.013) and apo A-I levels (&#946;st=-0.405, p=0.029) were independent predictors of cf-PWV in a model including hsCRP, HOMA-IR, and phenylacetate activity (r253 =0.42).Conclusions: High DAS-28 identifies patients with alterations in HDLcharacteristics. Plasma levels of apo A-I can be used as a marker of arterialstiffness in RA.","palabras_clave":"RHEUMATOID ARTHRITIS|HDL|PARAOXONASE|CHOLESTERYL ESTER TRANSFER PROTEIN|PULSE WAVE VELOCITY|ARTERIAL RIGIDITY|ATHEROSCLEROSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5192,"idioma_id":4,"titulo":"Adiabatic response and quantum thermoelectrics for ac-driven quantum systems","resumen":"We generalize the theory of thermoelectrics to include coherent electron systems under adiabatic ac driving, accounting for quantum pumping of charge and heat, as well as for the work exchanged bettween the electron system and driving potentials. We derive the relevant response coefficients in the adiabatic regime and show that  they  obey  generalized  Onsager  reciprocity  relations.  We  analyze  the  consequences  of  our  generalized thermoelectric framework for quantum motors, generators, heat engines, and heat pumps, characterizing them in terms of efficiencies and figures of merit. We illustrate these concepts in a model for a quantum pump.","palabras_clave":"QUANTUM TRANSPORT|THERMOELECTRICS|ADIABATIC MOTORS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5193,"idioma_id":4,"titulo":"Interaction of bovine viral diarrhea virus (BVDV) with bovine cumulus-oocyte complex during in vitro maturation: Detection in permissive cells","resumen":"Structural changes in the zona pellucida (ZP) of bovine oocytes seem to modulate their interaction with various viral agents, facilitating the viral infection in in vitro production systems. To evaluate the susceptibility of bovine oocytes to non-cytopathogenic bovine viral diarrhea virus (ncp-BVDV), cumulus-oocyte complexes were exposed to 107 TCID50\/ml of a ncp-BVDV strain during in vitro maturation. After that, cumulus cells and the ZP were removed by hyaluronidase and pronase treatment, respectively and the percentages of oocytes with polar body were analysed as a sign of nuclear maturation. After passage through cell culture, the virus was isolated from granulosa cells, ZP-free mature oocytes and ZP-intact mature oocytes. These results were confirmed by rtPCR. After consecutive washes, the virus remained associated to ZP-free oocytes, maintaining its replication and infectivity in permissive cells. Based on these findings it is concluded that the classical viral isolation procedure has a predictive value to detect BVDV associated to ZP-free oocytes and that it was novelty demonstrated that both washing and trypsin treatment of oocytes were ineffective to remove BVDV infection.","palabras_clave":"BOVINE VIRAL DIARRHEA VIRUS|BOVINE CUMULUS-OOCYTE COMPLEX|IN VITRO MATURATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5194,"idioma_id":4,"titulo":"Electronic properties and photofragmentation mechanisms of pyrosulfuryl chloride, ClSO2OSO2Cl","resumen":"The first ionization potential of ClSO2OSO2Cl, was determined by photoelectron spectroscopy at 12.25 eV. The photoelectron spectrum was interpreted, with the aid of DFT calculations, as composed by twelveionizations of non-bonding electrons localized on the oxygen and chlorine atoms. Several resonant transitions of inner electrons to LUMOs were detected in the Total Ion Yield spectra taken with tuneable synchrotron radiation. Photofragmentation mechanisms of pyrosulfuryl chloride after single and double ionization were studied by means of coincidence techniques (PEPICO, Photoelectron-Photoion-Coincidence and PEPIPICO, Photoelectron-Photoion-Photoion-Coincidence). The main fragmentationmechanism in the valence energy region leads to the formation of ClSO2OSO2+ fragment and a chlorine radical. Other fragments, like ClSO2+, SO2+, ClO+ (arising from an atomic rearrangement) and SO3+ wereobserved to appear as the energy of the synchrotron light increases. The Fragmentation channels after double ionization processes were inferred from the comparison of the shapes and experimental slopes of the coincidence islands for two ionic fragments originating from the same molecule in the PEPIPICO spectra, with the theoretical slopes calculated by the Eland?s formalism. The mechanisms were independent of the incident radiation energy, revealing the lack of site-specific processes. Allmechanisms were proposed to start from the rupture of either ClS or OS single bonds for a species which comprises and originates a number of relevant environmental and atmospheric processes and photoevolutions.","palabras_clave":"Photoionization|Photofragmentation|Photoelectron Spectroscopy|Synchrotron"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5195,"idioma_id":4,"titulo":"Structural Analysis of Perfluoropropanoyl Fluoride in the Gas, Liquid and Solid Phases","resumen":"The coexistence of two conformers in perfluoropropanoyl fluoride, CF3CF2C(O)F, differing in the CC-CF dihedral angle (gauche 85(10)% and anti 15(10)%), has been determined by means of gas-phase electron diffraction (GED). Quantum-chemical calculations performed at the MP2 and B3LYP approximations and cc-pVTZ basis sets reproduce the experimental values with confidence. By contrast, FTIR spectra give no clear evidence for the anti-conformer in the gas phase. Information on this less abundant but stable rotamer is obtained from matrix-isolation\/FTIR spectroscopy and liquid Raman spectroscopy. In situ crystallization and single-crystal X-ray diffraction (XRD) data reveal the presence of solely the gauche-conformation in the solid state. A set of intermolecular interactions including C=O&#8943;C=O, C-F&#8943;F-C, and F&#8943;C=O is detected. The nature of bonding and the relative stabilities of gauche- and anti-conformers are explored using natural bond orbitals.","palabras_clave":"Chemical bonds|Dihedral angle|Fluorine compounds|Gases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5196,"idioma_id":4,"titulo":"Vibrational spectra, conformational properties and argon matrix photochemistry of diacetyl diselenide, CH3C(O)Se2C(O)CH3","resumen":"By oxidation of selenoacetic acid, CH3C(O)SeH, we have prepared the hitherto unknown diacetyl diselenide, [CH3C(O)Se]2. Its vibrational properties were studied experimentally, both in neat liquid (Fourier transform infrared spectroscopy and Raman) with the molecule isolated in argon matrix at low temperatures and theoretically using MP2 and B3LYP methods in combination with 6-31+G(d), 6-311++G(3df,3dp) or aug-cc-pvDZ basis sets. Analysis of Ar matrix spectra reveals a conformational equilibrium in gas phase at room temperature, which was interpreted by considering only two of the three stable rotamers predicted by our three-dimensional potential energy surface scans. Energetic properties of three minima found theoretically were further studied in terms of donor&ndash;acceptor interactions by using natural bond orbital calculations at the same levels of theory. From this, it was possible to rationalize the conformational stability order and molecular structures by means of vicinal hyperconjugative delocalizations involving lone pairs on selenium or oxygen atoms and C=O, C&ndash;C or Se&ndash;C antibonding orbitals. The photochemistry of the compound in the argon matrix in the range between 200 and 800 nm was also investigated, revealing only one photochemical path to produce ketene, H2C=C=O, methylselane, CH3SeH, and carbonyl selenide, OCSe. Copyright &copy; 2016 John Wiley &amp; Sons, Ltd.","palabras_clave":"diselenide| hyperconjugation|matrix photochemistry|photodecomposition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5197,"idioma_id":4,"titulo":"ABOVEGROUND NET PRIMARY PRODUCTIVITY AND COVER OF VEGETATION EXPOSED TO VARIOUS DISTURBANCES IN ARID ARGENTINA","resumen":"We contrasted total herbaceous ANPP and cover between various disturbance types (Control, Burning, Shrub Control, Old Field 1, Old Field 2, Overgrazing) at a functional group (CSPG= cool-season perennial grasses; WSPG= warm-season perennial grasses; CSAG + D= cool-season annual grasses + dicots) and a species scale during 1984-1992. Results showed that (1) precipitation appeared to be more important than disturbance type in determining total ANPP (CSPG + WSPG + CSAG + Dicots); (2) CSPG made the greatest (P0.05) ANPP most of the times during 1984-1992; (4) Total plant cover of CSAG+D was most often greater (P<0.05) than that of WSPG at the Old Field 1 and Overgrazing disturbances.","palabras_clave":"aboveground net primary productivity|plant cover|disturbances|late- and early-seral plant species"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5198,"idioma_id":4,"titulo":"Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells","resumen":"Phosphatidylcholine (PC) is the main constituent of mammalian cell membranes. Consequently, preservation ofmembrane PC content and composition ? PC homeostasis ? is crucial to maintain cellular life. PC biosyntheticpathway is generally controlled by CTP:phosphocholine cytidylyltransferase (CCT), which is considered therate-limiting enzyme. CCTá is an amphitropic protein, whose enzymatic activity is commonly associated withendoplasmic reticulum redistribution. However, most of the enzyme is located inside the nuclei. Here, we demonstratethat CCTá is the most abundant isoform in renal collecting duct cells, and its redistribution is dependenton endogenous prostaglandins. Previously we have demonstrated that PC synthesis was inhibited by indomethacin(Indo) treatment, and this effectwas reverted by exogenous PGD2. In this work we found that Indo inducedCCTá distribution into intranuclear Lamin A\/C foci. Exogenous PGD2 reverted this effect by inducing CCTá redistributionto nuclear envelope, suggesting that PGD2 maintains PC synthesis by CCTá mobilization. Interestingly,we found that the effect of PGD2 was dependent on ERK1\/2 activation. In conclusion, our previous observationsand the present results lead us to suggest that papillary cells possess the ability to maintain their structural integritythrough the synthesis of their own survival molecule, PGD2, by modulating CCTá intracellular location","palabras_clave":"CTP:PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE ALPHA|PROSTAGLANDIN D2|ENZYME DISTRIBUTION CHANGES|NUCLEAR LOCALIZATION|ERK1\/2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5199,"idioma_id":4,"titulo":"Pathogenicity of Fusarium graminearum and F. meridionale on soybean pod blight and trichothecene accumulation","resumen":"Soybean (Glycine max) is the most important crop in Argentina. At present Fusarium graminearum is recognized as a primary pathogen of soybean in several countries in the Americas, mainly causing seed and root rot and pre- and post-emergence damping off. However, no information about infections at later growth stages of soybean development and pathogenicity of F. graminearum species complex is available. Therefore, the objectives of this study were to comparethe pathogenicity of F. graminearum and F. meridionale isolates towards soybean under field conditions and to evaluate the degree of pathogenicity and trichothecene production of these two phylogenetic species that express Different chemotypes. Six isolates of F. graminearum and F. meridionale were evaluated during 2012\/13 and 2013\/14 soybean growing seasons for pod blight severity, percentage of seed infected in pods and kernel weight reduction. The results showed a higher aggressiveness of both F. graminearum and F. meridionale species during the 2013\/14 season. However, the differences in pathogenicity observed between the seasons were not reflected in a distinct trichothecene con-centration in soybean seeds at maturity. Fusarium meridionale isolates showed similar pathogenicity to F. graminearum isolates but they were not able to produce this toxin in planta during the two field trials.","palabras_clave":"CHEMOTYPES|FUSARIUM GRAMINEARUM|SOYBEAN|PATHOGENICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5200,"idioma_id":4,"titulo":"Seed dormancy QTL identification across a Sorghum bicolor","resumen":"Pre-harvest sprouting (PHS) in Sorghum bicolor is one of the main constrains for its production in the central region of Argentina, as grain maturation often coincides with rainy or high environmental humidity conditions. The obtention of more dormant genotypes with higher PHS resistance has always been a desirable trait for breeders but the typical quantitative nature of seed dormancy makes its manipulation difficult through classical breeding. Dissecting this quantitative variability into quantitative trait loci (QTL) is a main concern especially in cereal species.In this work, a sorghum segregating population including 190 families was genotyped with microsatellite markers and the SbABI5 candidate gene. A genetic map encompassing 96 markers and a total length of 1331 cM was built. Seed dormancy was phenotyped in F3 and F4 panicles in two contrasting Argentinean environments (Castelar and Manfredi). Six seed dormancy QTL for mature grains were identified (qGI-1, qGI-3, qGI-4, qGI-6, qGI-7 and qGI-9) with the aid of QTL Cartographer and QTLNetwork, three of them (qGI-3, qGI-7 and qGI-9) being co-localised by both approaches. No epistasis was detected for the identified QTL but QTL-byenvironmentinteraction was significant for qGI-7 and qGI-9. Interestingly, seed dormancy candidate genes SbABI3\/VP1 and SbGA20ox3 were located within qGI-3, which makes them noteworthy candidate genes for this QTL.","palabras_clave":"Pre-harvest sprouting|QTL|Seed|Sorghum bicolor|SSRs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5201,"idioma_id":5,"titulo":"Zitrio la evolución en implantes de Zirconio","resumen":"En la Facultad de Odontología de La Plata se está trabajando comparativamente con los implantes de titanio evaluando los beneficios a la hora de la oseointegración de implantes de Zirconio mejorado con Itrio (Zitrio) mediante el estudio en superficie del implante. Las investigaciones actuales, van dirigidas a la obtención  de una cerámica que sea capaz de soportar las fuerzas oclusales, con una óptima estética y excelente biocompatibilidad.","palabras_clave":"ZITRIO|EVOLUCION|IMPLANTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5202,"idioma_id":4,"titulo":"Influence of the incorporation of fibers in biscuit dough on proton mobility characterized by time domain NMR","resumen":"The effect of fiber addition on the distribution and mobility of protons in biscuits is studied by using low resolution time domain nuclear magnetic resonance (TD-NMR). The proportion of flour is reduced in order to incorporate inulin and oat fiber. NMR temperature dependent experiments are carried out in order to gain insight on the processes occurring in biscuit baking. Proton populations were identified measuring spin?spin relaxation times (T2). The major change in the relaxation profiles upon incorporation of fibers corresponds to mobile water molecules, which appear to be related to dough spreading behavior and biscuit quality. Biscuit samples baked in a commercial oven were studied by two dimensional spin?lattice\/spin?spin (T1?T2) relaxation maps. The T1\/T2 ratio is used as an indicator of the population mobility, where changes in the mobility of water in contact with flour components as starch, proteins and pentosans are observed.","palabras_clave":"LOW RESOLUTIONPROTON NUCLEAR MAGNETIC RESONANCE|T1T2 CORRELATION MAPS|BISCUITS DOUGH|DIETARY FIBERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5203,"idioma_id":4,"titulo":"Use of enzymes to minimize the rheological dough problems caused by high levels of damaged starch in starch-gluten systems","resumen":"BACKGROUND: During wheat milling, starch granules can experience mechanical damage, producing damaged starch. High levels of damaged starch modify the physicochemical properties of wheat flour, negatively affecting the dough behavior as well as the flour quality and cookie and bread making quality. The aim of this work was to evaluate the effect of &#945;-amylase, maltogenic amylase and amyloglucosidase on dough rheology in order to propose alternatives to reduce the issues related to high levels of damaged starch.RESULTS: The dough with a high level of damaged starch became more viscous and resistant to deformations as well as less elastic and extensible. The soluble fraction of the doughs influenced the rheological behavior of the systems. The &#945;-amylase and amyloglucosidase reduced the negative effects of high damaged starch contents, improving the dough rheological properties modified by damaged starch. The rheological behavior of dough with the higher damaged-starch content was related to a more open gluten network arrangement as a result of the large size of the swollen damaged starch granules.CONCLUSION: We can conclude that the dough rheological properties of systems with high damaged starch content changed positively as a result of enzyme action, particularly &#945;-amylase and amyloglucosidase additions, allowing the use of these amylases and mixtures of them as corrective additives. Little information was reported about amyloglucosidase activity alone or combined with &#945;-amylase. The combinations of these two enzymes are promising to minimize the negative effects caused by high levels of damaged starch on product quality. More research needs to be done on bread quality combining these two enzymes. © 2015 Society of Chemical Industry.","palabras_clave":"enzymes|damged starch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5204,"idioma_id":4,"titulo":"Enhanced cyclooxygenase-2 expression levels and metalloproteinase 2  and 9 activation by Hexachlorobenzene in human endometrial stromal  cells","resumen":"Hexachlorobenzene (HCB) is an organochlorine pesticide that induces toxic reproductive effects in labo- ratory animals. It is a dioxin-like compound and a weak ligand of the aryl hydrocarbon receptor (AhR). Endometriosis is characterized by the presence of functional endometrial tissues outside the uterine cav- ity. Experimental studies indicate that exposure to organochlorines can interfere with both hormonal regulation and immune function to promote endometriosis. Altered expression of metalloproteinases (MMPs) in patients with endometriosis, suggests that MMPs may play a critical role. In the endometriotic lesions, prostaglandin E2 (PGE2) produced by cyclooxygenase-2 (COX-2), binds to its EP4 receptor (EP4), and via c-Src kinase induces MMPs activation, promoting endometriosis. We examined the HCB action on MMP-2 and MMP-9 activities and expression, COX-2 levels, PGE2 signaling, and the AhR involvement in HCB-induced effects. We have used different in vitro models: (1) human endometrial stromal cell line T-HESC, (2) primary cultures of Human Uterine Fibroblast (HUF), and (3) primary cultures of endometrial stromal cells from eutopic endometrium of control (CESC) and subjects with endometriosis (EESC). Our results show that HCB enhances MMP-2 and MMP-9 activities in T-HESC, HUF and ESC cells. The MMP-9 levels were elevated in all models, while the MMP-2 expression only increased in ESC cells. HCB enhanced COX-2 and EP4 expression, PGE2 secretion and the c-Src kinase activation in T-HESC. Besides, we observed that AhR is implicated in these HCB-induced effects. In conclusion, our results show that HCB exposure could contribute to endometriosis development, affecting inflammation and invasion parameters of human endometrial cells.","palabras_clave":"HEXACHLOROBENZENE|ENDOMETRIOSIS|METALLOPROTEINASE 2 AND 9|COX-2|PROSTAGLANDIN E2|EP2-EP4|c-Src KINASE|ARYL HYDROCARBON RECEPTOR|T-HESC HUMAN ENDOMETRIAL STROMAL CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5205,"idioma_id":4,"titulo":"Sensitivity of different soil quality indicators to assess sustainable land management: Influence of site features and seasonality","resumen":"The turnover rate of labile organic fractions varies continuously due to different soil uses and managements, weather conditions and sampling time. The aim of this study was to quantify the effect of different agricultural management, season and soil type on soil organic carbon (SOC) and its different fractions. The study was conducted on four sites located in the Argentinean Pampas. In each site, three treatments were defined: Good Agricultural Practices (GAP), Poor Agricultural Practices (PAP) and Natural Environment (NE). During two consecutive years (2010 and 2011) and at two different times (February and September) undisturbed soil samples were taken at 0-20 cm depth. Variables assessed included: SOC and its organic fractions: coarse (POCc) and fine (POCf) particulate organic carbon, SOC associated with a mineral fraction (MOC), total (CHt) and soluble (CHs) carbohydrates, bulk density (BD), and large pores (P>30). Also, indices associated with soil and management variables were determined. SOC reductions caused by agricultural practices were mainly from POCc. This fraction represented 34-52% and 50-74% for PAP and GAP, respectively, of the observed in NE. The carbon pool index (CPI) shows that agricultural treatments induced greater variations in all the labile organic fractions compared with SOC and MOC. In turn, the magnitude of variability was different among fractions, where temporal fluctuations increased according to the following order MOC 30), and indices (SOC: clay, structural index and intensification sequence index) were the variables that best explained the total variance of information of the four sites. Therefore, these indicators\/indices should be included in any minimum data set for evaluating the agricultural soil quality under no-tillage in the studied area.","palabras_clave":"Soil organic carbon|Organic fractions|Multivariate analysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5206,"idioma_id":4,"titulo":"Maximizing Information on the Environment by Dynamically Controlled Qubit Probes","resumen":"We explore the ability of a qubit probe to characterize unknown parameters of its environment. By resorting to the quantum estimation theory, we analytically find the ultimate bound on the precision of estimating key parameters of a broad class of ubiquitous environmental noises (?baths?) which the qubit may probe. These include the probe-bath coupling strength, the correlation time of generic types of bath spectra, and the power laws governing these spectra, as well as their dephasing times T2. Our central result is that by optimizing the dynamical control on the probe under realistic constraints one may attain the maximal accuracy bound on the estimation of these parameters by the least number of measurements possible. Applications of this protocol that combines dynamical control and estimation theory tools to quantum sensing are illustrated for a nitrogen-vacancy center in diamond used as a probe.","palabras_clave":"Quantum Information|Quantum sensing|Decoherence|Parameter estimation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5207,"idioma_id":5,"titulo":"LAS CLASES TEÓRICAS EN LA ENSEÑANZA DE LA HISTOLOGÍA Y EMBRIOLOGÍA BUCODENTAL: VALORACIÓN Y RENDIMIENTO.","resumen":"La clase teórica se define como una ?modalidad organizativa de la enseñanza cuya estrategia didáctica es la exposición verbal por parte del profesor de los contenidos sobre la materia objeto de estudio'.  Se  caracteriza por su unidireccionalidad, a la vez que facilita el abordaje de  mucha información (teoría) a un gran  número de alumnos. Si bien esta metodología se asocia a la enseñanza  tradicional donde el conocimiento está centrado en el profesor y los alumnos deben escuchar y repetir, puede ser utilizada con diversos fines didácticos tales como exponer los contenidos básicos relacionados con el tema de estudio, explicar la relación entre contenidos para facilitar su comprensión y aplicación, efectuar demostraciones (de casos, resolución de problemas), ilustrar la aplicación práctica de los contenidos. El objetivo del trabajo fue  relacionar la valoración que los estudiantes hicieron sobre las clases teóricas dictadas en  la Asignatura Histología y Embriología Bucodental con el rendimiento académico. Para evaluar el rendimiento académico se aplicó una prueba escrita que contenía preguntas conceptuales generales e imágenes para el reconocimiento e interpretación de estructuras histológicas, similares a las mostradas en las clases. Se utilizó la técnica de diferencial semántico mediante escalas de calificación gráfica de 5 grados para valorar la actitud de los alumnos en relación a las clases teóricas mediante un cuestionario anónimo aplicado a 220 cursantes de la materia. El 60% de los estudiantes estuvo de acuerdo con que se dicten  teóricos, el 67%  los consideró importantes para aprobar exámenes y facilitadores de la lectura de los libros; el 60% (aproximadamente) consideró que ayudan a la interpretación de imágenes y la organización del estudio. Menos de la mitad de los alumnos consideraron que las clases teóricas fueron interesantes (43,8%) resultando el porcentaje más bajo de las valoraciones positivas. El porcentaje general de valoraciones positivas para las clases teóricas fue del 59% mientras que las negativas, del 18%. De 161 estudiantes evaluados, el porcentaje de aprobados fue del 38,5% y desaprobados el 61,5%. Concluimos que  la clase teórica constituye un recurso didáctico  que facilita la  comunicación de conceptos pero debe complementarse con otras modalidades de enseñanza para mejorar el rendimiento.","palabras_clave":"CLASES TEÓRICAS |RENDIMIENTO ACADÉMICO|ENSEÑANZA|HISTOLOGÍA|EMBRIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5208,"idioma_id":4,"titulo":"Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines","resumen":"AbstractBackgroundAnaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species.MethodsSamples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis.ResultsAnaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca\/anteater isolate and the bovine group.ConclusionsIn the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology.","palabras_clave":"ANAPLASMA MARGINALE|WILD SPECIES|TARUCA|GIANT ANTIANTER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5209,"idioma_id":5,"titulo":"CITOTOXICIDAD DE UNA ASPARTIL PEPTIDASA DE Salpichroa origanifolia FRENTE A LA INFECCIÓN CAUSADA POR Phytophthora capsici EN ZAPALLITOS VERDES (Cucurbita maxima, var. Zapallito)","resumen":"Phytophthora capsici es un oomicete patógeno que causa importantes pérdidas en la producción de cultivos de interés agroeconómico. Los agroquímicos utilizados tradicionalmente para combatir estos fitopatógenos causan efectos adversos sobre la salud y el medio ambiente. Las plantas de la flora autóctona representan una fuente alternativa de metabolitos antifúngicos naturales. En nuestro laboratorio hemos estudiado la especie Salpichroa origanifolia, una hierba perenne autóctona del Norte y Centro de Argentina cuyos frutos, en forma de baya ovoide, son comestibles. A partir del extracto crudo de los frutos maduros de esta especie se purificó por cromatografía de intercambio iónico mediante un proceso en batch, una aspartil peptidasa a la cual denominamos salpichroína. El objetivo de este trabajo fue estudiar el efecto antifúngico de la enzima sobre el patógeno P. capsici. Salpichroína presentó un elevado efecto citotóxico sobre P. capsici en los ensayos in vitro, con un valor de CIM de 1,2 µmol L-1. Para evaluar el efecto de la enzima sobre el desarrollo de P. capsici en vegetales, se realizaron bioensayos de inoculación controlada de zapallitos verdes (Cucurbita máxima var Zapallito), observándose que los zapallitos inoculados con salpichroína junto con el fitopatógeno permanecieron asintomáticos durante siete días.","palabras_clave":"fungicidas|organismos patógenos|peptidasas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5210,"idioma_id":4,"titulo":"Aminotransferase and glutamate dehydrogenase activities in lactobacilli and streptococci","resumen":"Aminotransferases (ATs) and glutamate dehydrogenase (GDH) are two main types of enzymes involved in the initial steps of amino acid catabolism, which plays a key role in the cheese flavor development. In the present work, GDH and AT activities were screened in 21 strains of lactic acid bacteria of dairy interest, either cheese-isolated or commercial starters, including 15 mesophilic lactobacilli, four thermophilic lactobacilli, and two streptococci. The strains of Streptococcus thermophilus showed the highest GDH activity, which was significantly elevated compared with the lactobacilli. Aspartate AT prevailed in most strains tested, while the levels and specificity of other ATs were highly strain- and species-dependent. The knowledge of enzymatic profiles of these starter and cheese-isolated cultures is helpful in proposing appropriate combinations of strains for improved or increased cheese flavor.","palabras_clave":"AMINOTRANSFERASE ACTIVITY|GLUTAMATE DEHYDROGENASE ACTIVITY|LACTOBACILLI|STREPTOCOCCI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5211,"idioma_id":5,"titulo":"Agrandamiento gingival inducido por fármacos","resumen":"Se describe el Agrandamiento Gingival producidos por Fármacos. En este caso el Fármaco indicado es la Fenitoína, un anticonvulsivante frecuentemente indicado en distintos casos de Epilepsia. El caso clínico muestra a un joven paciente que después de ser operado de un Tumor Cerebral  ( Ependinoma) , presentó convulsiones que hicieron necesaria la indicación de esta droga. Como consecuencia de estose generó un importante Agrandamiento Gingival que fue derivado por el Medico Neurólogo tratante a nuestra Asignatura en la FOLP. Se describe el tratamiento quirúrgico efectuado y los controles en el tiempo. Un año y tres años.","palabras_clave":"Hiperplasia gingival|Fenitoína|Gingivectomía"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5212,"idioma_id":4,"titulo":"A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium Sinorhizobium meliloti","resumen":"Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixingsymbioses between rhizobia and their host legumes. Except for valuable molecular-biological studieson different rhizobia, no consolidated models have been formulated to describe the central physiologicchanges that occur in acid-stressed bacteria. We present here an integrated analysis entailing the maincultural, metabolic, and molecular responses of the model bacterium Sinorhizobium meliloti growingunder controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture mediumhad indicated that S. meliloti stopped growing at ca. pH 6.0?6.1. Under such a stress the rhizobiaincreased the O2 consumption per cell by more than 5-fold. This phenotype, together with an increase inthe transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acidstressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlatespecific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphatepathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses shouldbe focused on the time course of the observed changes, its associated intracellular signaling, and onthe comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) inrhizobia","palabras_clave":"Rhizobium|acid stress|omic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5213,"idioma_id":4,"titulo":"Spatial and temporal patterns of herbaceous primary production in semi-arid shrublands: a remote sensing approach","resumen":"Questions: Can herbaceous above-ground net primary production (ANPP) beestimated from remote sensing when woody and herbaceous plants are intermingled?How does herbaceous ANPP change in space and time in an ecosystemdominated by woody species? What are the main controls of herbaceous ANPPto paddock scale?Location: Native plant communities and buffelgrass roller chopped pastures of the Arid Chaco, western Argentina (28?32° S, 64?67° W; area: 100 000 km2).Methods: We decomposed normalized difference vegetation index (NDVI)data from MODIS (pixel size: 250 m 9 250 m) into woody (W) and herbaceous(H) components. We calibrated the relationship between field estimatesof herbaceous ANPP and the H component of NDVI using linearregression. The regression model fitted was applied to a 10-yr MODIS database for four paddocks to estimate herbaceous ANPP. We analysed the relationship between herbaceous ANPP and watering point distance andgrowing season precipitation.Results: The annual integral of NDVI 9 proportion of the herbaceous component [H\/(H + W)] explained 71% and 91% of herbaceous ANPP variationin native plant communities and buffelgrass roller chopped pastures,respectively. The regression model fitted, however, differed (P < 0.05)between the two types of system. The NDVI annual integral explained ahigher proportion of herbaceous ANPP variations than the NDVI annualpeak or the growing season (December?April) integral. For native plantcommunities, herbaceous production increased significantly (P < 0.05) with watering point distance, and marginally significantly (P < 0.10) with growing season precipitation. For buffelgrass roller chopped pastures, the herbaceous production increased significantly (P < 0.05) with growing seasonprecipitation.Conclusion: Our model was able to estimate herbaceous ANPP from the decomposition of an NDVI time series that included woody components. Thus, the model provides the basis for more accurate monitoring of spatial and temporal variability of herbaceous ANPP in areas where herbaceous and woody plant components co-exist. Applying our models, we detected clear spatial and temporal patterns of herbaceous ANPP. The possibility of describing in a spatially explicit way the past 14 yrs of herbaceous ANPP allows designing livestock management strategies and devise alternatives to control degradation processes in the Arid Chaco.","palabras_clave":"Above-ground net primary production|Grasses|Normalized difference vegetation index|Shrublands"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5214,"idioma_id":4,"titulo":"Lactobacillus fermentum CRL1446 ameliorates oxidative  and metabolic parameters by increasing intestinal  feruloyl esterase activity and modulating microbiota in caloric-restricted mice","resumen":"The purposeof this study was to determine whether the administration of the feruloylesterase (FE)-producing strain Lactobacillusfermentum CRL1446 enhances metabolic and oxidative parameters incaloric-restricted (CR) mice. Balb\/c male mice were divided into ad libitum fedGroup (ALF Group), CR diet Group (CR Group) and CR diet plus L. fermentum Group (CR-Lf Group). CRdiet was administered during 45 days and CRL1446 strain was given in the doseof 108 cells\/mL\/day\/mouse. FE activity was determined in intestinalmucosa and content at Day 1, 20 and 45. Triglyceride, total cholesterol, glucose,thiobarbituric acid reactive substances (TBARS) levels and glutathionereductase activity were determined in plasma. Gut microbiota was evaluated byhigh-throughput sequencing of 16S rRNA gene amplicons. At Day 45, totalintestinal FE activity in CR-Lf Group was higher (p = 0.020) than in CR and ALFgroups and an improvement in both metabolic (reductions in triglyceride (p = 0.0025), total cholesterol (p = 0.005) and glucose (p &lt; 0.0001) levels) and oxidative(decrease of TBARS levels and increase of plasmatic glutathione reductase activity(p = 0.006)) parameterswas observed, compared to ALF Group. CR diet increased abundance ofBacteroidetes and CRL1446 administration increased abundance of Bifidobacterium and Lactobacillus genus. L.fermentun CRL1446 exerted a bifidogenic effect under CR conditions.","palabras_clave":"CALORIC RESTRICTION DIET|LACTOBACILLUS FERMENTUM|INTESTINAL FERULOYL ESTERASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5215,"idioma_id":4,"titulo":"Anti-inflammatory and osteoprotective effects of cannabinoid-2 receptor agonist HU-308 in a rat model of lipopolysaccharide-induced periodontitis","resumen":"BACKGROUND:Anti-inflammatory and immunologic properties of cannabinoids have been reported in several tissues. Expression of cannabinoid receptor Type 2 was reported in osteoblasts and osteoclasts, suggesting a key role in bone metabolism. The aim of this study is to assess the effect of treatment with cannabinoid-2 receptor agonist HU-308 in the oral health of rats subjected to lipopolysaccharide (LPS)-induced periodontitis.METHODS:Twenty-four rats were distributed in four groups (six rats per group): 1) control rats; 2) sham rats; 3) rats submitted to experimental periodontitis (LPS); and 4) rats submitted to experimental periodontitis and treated with HU-308 (LPS+HU). In groups LPS and LPS+HU, periodontitis was induced by LPS (1 mg\/mL) injected into the gingival tissue (GT) of maxillary and mandibular first molars and into the interdental space between the first and second molars, 3 days per week for 6 weeks. In group LPS+HU, HU-308 (500 ng\/mL) was applied topically to the GT daily.RESULTS:Alveolar bone loss resulting from LPS-induced periodontitis was significantly attenuated with HU-308 treatment (LPS+HU), measured by macroscopic and histologic examination. Treatment also reduced gingival production of inflammatory mediators augmented in LPS-injected rats, such as: 1) inducible nitric oxide (iNOS) activity (LPS: 90.18 ± 36.51 pmol\/minute\/mg protein versus LPS+HU: 16.37 ± 4.73 pmol\/minute\/mg protein; P <0.05); 2) tumor necrosis factor alpha (LPS: 185.70 ± 25.63 pg\/mg protein versus LPS+HU: 95.89 ± 17.47 pg\/mg protein; P <0.05); and 3) prostaglandin E2 (PGE2) (LPS: 159.20 ± 38.70 pg\/mg wet weight versus LPS+HU: 71.25 ± 17.75 pg\/mg wet weight; P <0.05). Additionally, HU-308 treatment prevented the inhibitory effect of LPS-induced periodontitis on the salivary secretory response to pilocarpine. Moreover, iNOS activity and PGE2 content, which were increased by LPS-induced periodontitis in the submandibular gland, returned to control values after HU-308 treatment.CONCLUSION:This study demonstrates anti-inflammatory, osteoprotective, and prohomeostatic effects of HU-308 in oral tissues of rats with LPS-induced periodontitis.","palabras_clave":"ANTI-INFLAMMATORY AGENTS|CANNABINOID RECEPTOR AGONISTS|LIPOPOLYSACCHARIDE|PERIODONTITIS|SALIVA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5216,"idioma_id":4,"titulo":"Martensitic transformation strain and stability of Ni50-x-Ti50-Cox (x = 3, 4) strips obtained by twin-roll casting and standard processing techniques","resumen":"The martensitic transformation properties (maximum strain, residual strain and transformation stability) of Ni50&#8722;x?Ti50?Cox strips produced by twin-roll casting (TRC) and standard casting (SC) were compared. A complete microstructural characterization was carried out on both samples using optical microscopy, transmission electron microscopy (TEM), electron backscattering diffraction (EBSD-SEM), energy dispersive X-ray spectroscopy (EDS-SEM), X-Ray diffraction and differential scanning calorimetry (DSC). According to the results obtained by DSC and load-biased thermal-cycling measurements, the TRC strip is more stable and has lower residual strain than the SC strip for loads below 90 MPa. Using the austenitic texture of each strip, the recoverable strain upper bounds of the martensitic transformation (Sachs´ bound) were calculated. A comparison between the measured maximum recoverable strain and the Sachs´ bound, allows us to discuss how the particular microstructures produced by the two production techniques affect the strip´s shape memory properties.","palabras_clave":"NiTi ALLOYS|SHAPE MEMORY|TWIN-ROLL CASTING|MECHANICAL PROPERTIES|TEXTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5217,"idioma_id":4,"titulo":"Emerging Hubs in Plant Light and Temperature Signaling","resumen":"Due to their nature as sessile organisms, plants must accurately sense their surroundings and then translate this information into efficient acclimation responses to maximize development. Light and temperature are two major stimuli that provide immediate cues regarding energy availability, daylength, proximity of other species and seasonal changes. Both cues are sensed by complex systems and the integration of these signals is of very high value to properly respond to environmental changes without being disguised by random changes. For instance a cold day has a different significance if it occurs during the illuminated phase of the day or during the night, or when days are shortening during the fall instead of a long-day in spring. Here, we summarize recent advances in the nature of signaling components that operate as connectors of light and temperature signaling, with emphasis on the emerging hubs. Despite the nature of the thermosensors is still in its infancy compared to an important body of knowledge about plant sensory photoreceptors, the interaction of both types of signaling will not only bring clues of how plants integrate environmental information, but also will help in leading research in the nature of the thermosensors themselves.","palabras_clave":"Plant|Light|Temperature"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5218,"idioma_id":4,"titulo":"Chlorophyll fluorescence, photochemical reflective index and normalized difference vegetative index during plant senescence","resumen":"tThe relationship between the Photochemical Reflectance Index (PRI), Normalized Difference VegetationIndex (NDVI) and chlorophyll fluorescence along senescence was investigated in this work. Reflectanceand radiance measurements were performed at canopy level in grass species presenting different pho-tosynthetic metabolism: Avena sativa (C3) and Setaria italica (C4), at different stages of the naturalsenescence process. Sun induced-chlorophyll fluorescence at 760 nm (SIF760) and the apparent fluo-rescence yield (SIF760\/a, with a = irradiance at time of measurement) were extracted from the radiancespectra of canopies using the Fraunhofer Line Discrimination-method. The photosynthetic parametersderived from Kautsky kinetics and pigment content were also calculated at leaf level.Whilst stand level NDVI patterns were related to changes in the structure of canopies and not inpigment content, stand level PRI patterns suggested changes both in terms of canopy and of pigmentcontent in leaves. Both SIF760\/a and PSIIdecreased progressively along senescence in both species. Astrong increment in NPQ was evident in A. sativa while in S. italica NPQ values were lower. Our mostimportant finding was that two chlorophyll fluorescence signals, PSIIand SIF760\/a, correlated with thecanopy PRI values in the two grasses assessed, even when tissues at different ontogenic stages werepresent. Even though significant changes occurred in the Total Chlr\/Car ratio along senescence in bothstudied species, significant correlations between PRI and chlorophyll fluorescence signals might indicatethe usefulness of this reflectance index as a proxy of photosynthetic RUE, at least under the conditions ofthis study. The relationships between stand level PRI and the fluorescence estimators (PSIIand SIF760\/a)were positive in both cases. Therefore, an increase in PRI values as in the fluorescence parameters wouldindicate higher RUE.","palabras_clave":"PHOTOCHEMICAL REFLECTANCE INDEX|NORMALIZED DIFFERENCE VEGETATION INDEX|SUN-INDUCED CHLOROPHYLL FLUORESCENCE |RADIATION USE EFFICIENCY |PHOTOSYNTHETHIC PARAMETERS |SENESCENCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5219,"idioma_id":4,"titulo":"Geometric evolution of the Horcones Inferior Glacier (Mount Aconcagua, Central Andes) during the 2002-2006 surge","resumen":"The Central Andes of Chile and Argentina (31?35°S) contain a large number and variety of ice masses, but only two surging glaciers have been studied in this region. We analyzed the 2002?2006 surge of the Horcones Inferior Glacier, Mount Aconcagua, Argentina, based on medium spatial resolution (15?30m) satellite images and digital elevation models. During the buildup phase the glacier was stagnant, with velocities lower than 0.1 m\/d. In the active-phase velocities reached 14 m\/d and the glacier front advanced 3.1 km. At the peak of the active phase (2003?2004), the area-averaged elevation change was &#8722;42 m in the reservoir zone (2.53 km2) and +30 m in the receiving zone (3.31 km2). The estimated ice flux through a cross section located at 4175 meter above sea level was 108 m3 during a period of 391 days, a flux that suggests a mean glacier thickness at this location of ~90 m. The depletion phase showed a recovery of the reservoir zone elevation, the down wasting of the receiving zone (&#8722;17 m, 2007?2014), and a return to quiescent velocities. The short active phase, the abrupt change in the velocities, and the high level of the proglacial stream indicate a hydrological switch (Alaska type) trigger. The 2002?2006 and 1984?1990 surges of Horcones Inferior were synchronous with the surges of nearby Grande del Nevado Glacier. These events occurred after periods of positive mass balance, so we hypothesize a climate driver.","palabras_clave":"HORCONES INFERIOR|GLACIER SURGE|ASTER|CENTRAL ANDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5220,"idioma_id":4,"titulo":"Reconstructing the annual mass balance of the Echaurren Norte glacier (Central Andes, 33.5° S) using local and regional hydroclimatic data","resumen":"Despite the great numberand variety of glaciers in southern South America,in situ glacier mass balance recordsare extremely scarce and glacier-climate relationships are still poorlyunderstood in this region. Here we use the longest (&gt;35 yrs) and most completein situ mass balance record, availablefor glaciar Echaurren Norte in the Andes at ~33.5°S, to develop a minimal glaciersurface mass balance model that relies on nearby monthly precipitation and airtemperature data as forcing. This basic model is able to explain 78% of the variancein the annual glacier mass balance record over the 1978-2013 calibration period.An attribution assessment identified precipitation variability as the dominantforcing modulating annual mass balances at ECH, with temperature variationslikely playing a secondary role. A regionally-averaged series of mean annualstreamflow records from both sides of the Andesbetween ~30° and 37°S is then used to estimate, through simple linearregression, this glacier?s annual mass balance variations since 1909. Thereconstruction model captures 68% of the observed glacier mass balancevariability and shows three periods of sustained positive mass balances embeddedin an overall negative trend over the past 105 years. The three periods of sustainedpositive mass balances (centered in the 1920s-30s, in the 1980s and in thefirst decade of the 21st century) coincide with several documented glacieradvances in this region. Similar trends observed in other shorter glacier massbalance series suggest that the glaciar Echaurren Norte reconstruction is representativeof larger-scale conditions and could be useful for more detailed glaciological,hydrological and climatological assessments in this portion of the Andes.","palabras_clave":"MASS BALANCE|ECHAURREN NORTE|HYDRO-CLIMATIC DATA|MASS BALANCE MODEL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5221,"idioma_id":4,"titulo":"Neglected Tropical Protozoan Diseases: Drug Repositioning as a Rational Option","resumen":"Abstract: Neglected tropical diseases represent a major sanitary problem and a huge economic burden to endemic countries, and are currently expanding to non-endemic countries owing to migration currents. Though long abandoned in the past, recent research on novel therapeutics has already started to show results. Drug repositioning is one of the prominent, more successful strategies to approach the development of new treatments for these diseases. Here we present an overview on the limitations of the current available medications to treat African trypanosomiasis, Chagas disease and Leishmaniasis, along with a review on drug candidates presently undergoing clinical trials and drug candidates identified through drug repositioning initiatives.","palabras_clave":"DRUG|REPOSITIONING|PROTOZOAN|DISEASES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5222,"idioma_id":4,"titulo":"Influence of raw material moisture on the synthesis of black tea production process","resumen":"Tea industry is one of the main activities in the northeast of Argentina. It presents some particularities respect of tea production in other regions of the world, mainly in the high level of automation in tea shoots harvesting. Besides, the factory configurations in terms of the equipment used in tea production are different and it is not clear if they are efficient or not. The objective of this work is to study the effects of the moisture in the raw material (tea shoots) in the optimal design of a tea manufacturing plant not only in the investment but also in the operation costs. A superstructure that includes all the equipment involved in black tea manufacturing is proposed and a Generalized Disjunctive Programming model (GDP) is formulated to find the optimal flow sheet to produce black tea minimizing investment and operation cost, considering different wet contents in the tea shoots. A comparisonwas also made among the optimal plant and typical factories configurations to analyze their performance. From the results obtained can be observed that if the moisture of raw material is below of 4.5 kg of water per kg of dry matter the optimal plant configuration does not change; above this value more investment is needed to extract the extra water content. A similar conclusion can be obtained from the analysis of the operation costs. The optimal configuration is formed by conventional preservation chambers, continuous withering belts, Rotorvane crusher, continuous fermenting machines and fluid bed dryers. Comparing other typical configurations against the optimal one, the operative unit cost is about 20% - 60% greater.","palabras_clave":"Black tea production|Raw material moisture|Process system optimization|Disjunctive Programming"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5223,"idioma_id":4,"titulo":"Efficient and selective N-, S- and O-acetylation in TEAA ionic liquid as green solvent. Applications in synthetic carbohydrate chemistry","resumen":"he ionic liquid triethylammonium acetate (TEAA) was found to be an efficient solvent in the acetylation of alcohols, amines, oximes and thiols to their corresponding acetyl compounds using only a 10% excess of acetic anhydride under mild conditions. Moreover TEAA is not only an inexpensive and recyclable solvent but also an anomeric selective catalyst in the per-Oacetylation of sugar moieties.","palabras_clave":"ACYLATION|CARBOHYDRATES|GREEN CHEMISTRY|TEAA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5224,"idioma_id":4,"titulo":"Release of outer membrane vesicles in Pseudomonas putida as a response to stress caused by cationic surfactants","resumen":"Pseudomonas putida A (ATCC 12633), a degrader of cationic surfactants, releases outer membrane vesicles (OMVs) when grows with tetradecyltrimethylammonium bromide (TTAB) as sole carbon, nitrogen and energy source. The OMVs exhibit a bilayer structure and were found to be composed of lipopolysaccharide, proteins and phospholipids such as cardiolipin, phosphatidylcholine, phosphatidic acid and phosphatidylglycerol. The OMVs showed a marked increase in the phosphatidylglicerol content, approximately 43% higher than the amount registered in the parent cells from which the vesicles derived. After growth of P. putida with TTAB, the amount lipoprotein covalently cross-linked to the peptidoglycan showed a 2-fold decrease when compared with values found after growth without the surfactant (16± 2 and 28± 3 µg (mg cell envelope protein)-1, respectively). This decrease in the amount of lipoprotein can be related to areas of loss of contact between the outer membrane and the peptidoglycan and, therefore, to OMVs production. In addition, due to its amphiphilic nature, TTAB can contribute with the OMVs biogenesis, through a physical mechanism, by induction of the curvature of the membrane. Taking into account that OVMs were produced when the cells were grown under the external stress, caused by the surfactant, and that TTAB was detected in the vesicles (48 nmol TTAB nmol phospholipids-1), we conclude that this system of TTAB elimination is a mechanism that P. putida A (ATCC 12633) would utilize for alleviating stress caused by cationic surfactants","palabras_clave":"Vesicles|Pseudomonas putida|stress|TTAB"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5225,"idioma_id":4,"titulo":"The chronic toxicity of pani-NPS to the larvae stage of Rhinella arenarum","resumen":"The interaction between live organisms and nanosized materials has become a current focus in toxicology. The aim of this study was to establish the chronic effects of PANI-Nps in different dispersant on Rhinella arenarum toad larvae. We propose the use of organism´s models to understand the toxic mechanistic modes of action that could occur by exposure to Nps. AMPHITOX is proving to be a quick, cheap, and facile model to conservatively assess toxicity of nanomaterials. The toxicity of both: PANI-Np1 (PANI-Np dispersed in PVP) and PANI-Np2 (PANI-Np dispersed in PVP+PNIPAM) was time- and concentration-dependent. The range of concentrations used were 182-980 mg\/L for PANI-Np1 or 250-1000 mg\/L for PANI-Np2 and the larval stage used was S.25 (closed branchiae). PANI-Np2 was more toxic than PANI-Np1 based on the chronic toxicity results. At stage S.25, no alterations in the larvae skin were observe while nanoparticle precipitations were detected into the digestive tract; suggesting that PANI nanoparticles are probaly unable to enter through the oral route.","palabras_clave":"CHRONIC TEST|AMPHIBIAN LARVAE|POLYANILINE NANOPARTICLES|ENVIRONMENTAL POLLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5226,"idioma_id":4,"titulo":"Rheological characterization of full-fat and low-fat glaze materials for foods","resumen":"The objective of this work was to perform the rheological characterization of full-fat and low-fat glaze materials used as film-forming fluids. Glaze materials were elaborated with icing sugar, skim milk &nbsp;&nbsp; powder, water, and different contents (0, 3, 6, and 9%) of vegetable fat (sunflower oil and soy lecithin) or microparticulated whey protein as fat mimetic. Rotational rheometry, creep tests, and dynamical rheometry were performed. Yield stress was obtained by direct and indirect methods. Steady shear viscosity was analyzed applying the HerscheleBulkley model. Complex moduli were modeled with a power law equation and the application of Cox-Merz rule was considered. Images were obtained by optical microscopy. Average film thickness values were estimated using a mechanistic mathematical model for a dip-coating process during the draining stage. The results indicated that it may be possible to obtain low-fat glaze materials with rheological properties, microstructure, and film thickness-forming capacities similar to full-fat formulations.","palabras_clave":"RHEOLOGY|GLAZE MATERIAL|FOOD COATING|FAT MIMETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5227,"idioma_id":4,"titulo":"Herbivory and the success of Ligustrum lucidum: evidence from a comparison between native and novel ranges","resumen":"Invasive plant species may benefit from a reduction in herbivory in their introduced range. The reducedherbivory may cause a reallocation of resources from defence to fitness. Here, we evaluated leaf herbivory of an invasivetree species (Ligustrum lucidum Aiton) in its native and novel ranges, and determined the potential changes in leaf traitsthat may be associated with the patterns of herbivory. We measured forest structure, damage by herbivores and leaf traitsin novel and native ranges, and on the basis of the literature, we identified the common natural herbivores of L. lucidum.We also performed an experiment offering leaves from both ranges to a generalist herbivore (Spodoptera frugiperda).L. lucidum was more abundant and experienced significantly less foliar damage in the novel than in the native range, in spiteof the occurrence of several natural herbivores. The reduced lignin content and lower lignin :N ratio in novel leaves,together with the higher herbivore preference for leaves of this origin in the laboratory experiment, indicated lower herbivoreresistance in novel than in native populations. The reduced damage by herbivores is not the only factor explaining invasionsuccess, but it may be an important cause that enhances the invasiveness of L. lucidum.","palabras_clave":"biological invasions|invasive woody species|natural enemies release hypothesis|EICA hypothesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5229,"idioma_id":4,"titulo":"Surface modification of multiwall carbon nanotubes by sulfonitric treatment","resumen":"Carbon nanotubes are widely used for electronic, mechanical, and optical devices due to their unique structural and quantum characteristics. The species generated by oxidation on the surface of these materials permit binding new reaction chains, which improves the dispersibility, processing and compatibility with other materials. Even though different acid treatments and applications of these CNT have been reported, relatively few research studies have focused on the relationship between the acid treatment and the formation of nanodefects, specific oxidized species or CNT surface defects. In this work, multiwall carbon nanotube (MWCNT) oxidation at 90°C was characterized in order to determine the acid treatment effect on the surface. It was found that oxidized species are already present in MWCNT without an acid treatment, but there are not enough to cause water-based dispersion. The species were identified and quantified by infrared spectroscopy and X-ray photoelectron spectroscopy. Also, transmission electron microscopy observations showed not only modifications of the oxidized species, but also morphological damage on the surfaces of MWCNT after being subjected to the acid treatment. This effect was also confirmed by Raman spectroscopy. The acid treatment generates higher oxidized species, decreasing the zeta potential in the whole pH range.","palabras_clave":"ACID TREATMENT|CARBON NANOTUBES|SURFACE MODIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5230,"idioma_id":4,"titulo":"Testing alien plant distribution and habitat invasibility in mountain ecosystems: growth form matters","resumen":"Most studies focused on understanding habitat invasibility use the current levels of invasion as a direct proxy of habitat invasibility. This has shown to be biased by the influence of propagule pressure and climate. We suggest that plant growth forms need to be considered as an extra factor, as habitat preferences might not be equal for all potential invaders. We test the influence of propagule pressure, climate and habitat characteristics on the current level of invasion and habitat invasibility, specifically addressing whether an analysis focused on growth forms evidence different patterns than the total pool of alien species. We used 499 floristic vegetation plots located in Córdoba Mountains. We used proportional alien richness of the total pool and for each growth form as response variables. We identified models that best explained current levels of invasion. We used the residuals of the models with propagule pressure and climate as the response variable. Then, we performed linear models to test the relationship between habitat characteristics and the residuals of the models. We found different drivers of current alien distribution patterns for the total pool and each growth form. Habitat invasibility was not equal when quantified for the total pool or growth forms. Shrublands and outcrops were recorded as less susceptible to woody invasion, while grasslands and native woodlands were resistant to the invasion of grasses and none habitat type was resistant to the invasion of forbs. We highlight that the current level of invasion and habitat invasibility are highly growth form dependent.","palabras_clave":"ALIEN SPECIES|CLIMATE|EXOTIC|HABITAT CHARACTERISTICS|PROPAGULE PRESSURE|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5231,"idioma_id":4,"titulo":"Response to Vergara et al. (2015)?Fruiting phenology as a ??triggering attribute?? of invasion process: Do invasive species take advantage of seed dispersal service provided by native birds?","resumen":"ES UNA NOTA SIN RESUMEN.(...) In summary, while fully recognizing the value of Vergara et al.?s (2015) results in documenting and explaining present patterns of bird-plant interactions, we believe they do not reject the TA model (sensu Gurvich et al. 2005) as claimed by the authors. In the case of P. angustifolia, the empirical findings actually support the model. In the case of P. coccinea, they highlight that another  attribute might be triggering invasive success, which could be true although moreresearch is needed to confirm this assertion.","palabras_clave":"TRIGGERING ATTRIBUTE|PYRACANTHA ANGUSTIFOLIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5232,"idioma_id":4,"titulo":"The Role of Endothelin System in Renal Structure and Function during the Postnatal Development of the Rat Kidney","resumen":"Renal development in rodents, unlike in humans, continues during early postnatal period.We aimed to evaluate whether the pharmacological inhibition of Endothelin system duringthis period affects renal development, both at structural and functional level in male andfemale rats. Newborn rats were treated orally from postnatal day 1 to 20 with vehicle orbosentan (Actelion, 20 mg\/kg\/day), a dual endothelin receptor antagonist (ERA). The animals were divided in 4 groups: control males, control females, ERA males and ERAfemales. At day 21, we evaluated renal function, determined the glomerular number by amaceration method and by morphometric analysis and evaluated possible structural renalalterations by three methods:halphai-Smooth muscle actin (&#945;-SMA) immunohistochemistry, Masson´s trichrome and Sirius red staining. The pharmacological inhibition of Endothelin system with a dual ERA during the early postnatal period of the rat did not leads to renaldamage in the kidneys of male and female rats. However, ERA administration decreasedthe number of glomeruli, the juxtamedullary filtration surface area and the glomerular filtration rate and increased the proteinuria. These effects could predispose to hypertension or renal diseases in the adulthood. On the other hand, these effects were more pronounced inmale rats, suggesting that there are sex differences that could be greater later in life. Theseresults provide evidence that Endothelin has an important role in rat renal postnatal development. However these results do not imply that the same could happen in humans, since human renal development is complete at birth.","palabras_clave":"ENDOTHELIN|RENAL POSTNATAL DEVELOPMENT|ENDOTHELIN RECEPTOR ANTAGONIST|BOSENTAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5233,"idioma_id":4,"titulo":"A generalized effective anisotropic poroelastic model for periodically  layered media accounting for both Biot's global and interlayer flows","resumen":"We present a generalized effective poroelastic model for periodically layered media in the mesoscopic scale range, which accounts for both Biot?s global and interlayer wave-induced fluid flow, as well as for the anisotropy associated with the layering. Correspondingly, it correctly predicts the existence of the fast and slow P-waves as well as quasi and pure S-waves. The proposed analytical model is validated through comparisons of the P-wave and S-wave phase velocity dispersion and attenuation characteristics with those inferred from a one-dimensional numerical solution of Biot?s poroelastic equations of motion. We also compare our model with the classical mesoscopic model of White for a range of scenarios. The results demonstrate that accounting for both wave-induced fluid flow mechanisms is essential when Biot?s global flow prevails at frequencies that are comparable or smallerwith respect to those governing interlayer flow. This is likely to be the case in media of high permeability, such as, for example, unconsolidated sediments, clean sandstones, karstic carbonates, or fractured rocks. Conversely, when interlayer flow occurs at smaller frequencies with respect to Biot?s global flow, the predictions of this model are in agreement with White?s model, which is based on quasi-static poroelasticity.","palabras_clave":"ROCK PHYSICS|MATHEMATICAL FORMULATION|ATTENUATION|ANISOTROPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5234,"idioma_id":4,"titulo":"Agricultural  expansion  in  the  Semiarid  Chaco:  Poorly  selective contagious  advance","resumen":"Clearance  for  agriculture  or  cattle  ranching  was  the  dominant  land-cover  change  during  the  last  two decades  in  the  South  American  Dry  Chaco.  The  Argentinean  portion  has  been  particularly  affected,  presenting  greater  deforestation  rates  than  the  continental  and  global  averages.  Little  is  known  on  the  control factors  of  the  location  and  the  spatial  clearance  patterns.  In  this  article  we  studied  (a)  deforestation dynamics  in  the  Argentinean  Dry  Chaco  and  the  factors  determining  land  clearing  locations  for  the  last 25  years;  (b)  changes  in  the  relative  impact  of  those  factors  through  time  and  space;  and  (c)  the  effect of  regulations  aimed  to  control  the  location  and  magnitude  of  land  transformation.  We  also  tested  the ?expansion  of  the  agricultural  frontier?  hypothesis  for  the  Argentinean  Chaco.  To  identify  the  factors  that defined  agricultural  expansion  we  used  binomial  logistic  models  that  were  fitted  to  a  set  of  independent variables  (bio-physical,  infrastructure  and  political  factors)  that  could eventually  influence  the  distribution  of  new  agricultural  areas.  Results  indicate  that  the  Forest  Law  devised  by  the  Argentinean  federal government  to  control  the  clearing  process  was  insufficient  to  restrict  both  the  area  transformed  per  year and  clearance  locations.  Agriculture  is  expanding  over  marginal  areas  and  land  clearing  dynamics  have been  increasingly  associated  to  the  proximity  to  already  cleared  areas,  defining  a  frontier?advancement pattern  which  gives  the  idea  of  a  contagion  process.  According  to  our  results,  the  relative  importance  ofthe  anthropic  factors  associated  to  the  agricultural  expansion  in  the  region  increased  through  time.","palabras_clave":"Deforestation|Land  use  change Models|Dry  Chaco|Remote  sensing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5235,"idioma_id":4,"titulo":"PLEIOTROPIC EFFECTS OF 5-AMINOLEVULINIC ACID IN MICE BRAIN","resumen":"5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg\/kg body mass) or multiple doses of ALA (40 mg\/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage.","palabras_clave":"5-AMINOLEVULINIC ACID|HEME METABOLISM|ANTIOXIDANT DEFENSE SYSTEM|CHOLINERGIC SYSTEM|NITRIC OXIDE SYNTHASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5237,"idioma_id":4,"titulo":"Role of Actin Cytoskeleton During Mammalian Sperm Acrosomal Exocytosis","resumen":"Mammalian sperm require to undergo an exocytotic process called acrosomal exocytosis in order to be able to fuse with the oocyte. This ability is acquired during the course of sperm capacitation. This review is focused on one aspect related to this acquisition: the role of the actin cytoskeleton. Evidence from different laboratories indicates that actin polymerization occurs during capacitation, and the detection of several actin-related proteins suggests that the cytoskeleton is involved in important sperm functions. In other mammalian cells, the cortical actin network acts as a dominant negative clamp which blocks constitutive exocytosis but, at the same time, is necessary to prepare the cell to undergo regulated exocytosis. Thus, F-actin stabilizes structures generated by exocytosis and supports the physiological progression of this process. Is this also the case in mammalian sperm? This review summarizes what is currently known about actin and its related proteins in the male gamete, with particular emphasis on their role in acrosomal exocytosis.","palabras_clave":"SPERM|ACTIN|CYTOSKELETON|ACROSOMAL EXOCYTOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5238,"idioma_id":4,"titulo":"Dopaminergic drugs in type 2 diabetes and glucose homeostasis","resumen":"The importance of dopamine in central nervous system function is well known, but its effects on glucose homeostasis and pancreatic &#946; cell function are beginning to be unraveled. Mutant mice lacking dopamine type 2 receptors (D2R) are glucose intolerant and have abnormal insulin secretion. In humans administration of neuroleptic drugs, which block dopamine receptors, may cause hyperinsulinemia, increased weight gain and glucose intolerance. Conversely, treatment with the dopamine precursor L-DOPA in patients with Parkinson?s disease reduces insulin secretion upon oral glucose tolerance test, and bromocriptine improves glycemic control and glucose tolerance in obese type 2 diabetic patients as well as in non diabetic obese animals and humans. The actions of dopamine on glucose homeostasis and food intake impact both the autonomic nervous system and the endocrine system. Different central actions of the dopamine system may mediate its metabolic effects such as: i) regulation of hypothalamic noradrenaline output, ii) participation in appetite control, and iii) maintenance of the biological clock in the suprachiasmatic nucleus. On the other hand, dopamine inhibits prolactin, which has metabolic functions; and, at the pancreatic beta cell dopamine D2 receptors inhibit insulin secretion. We review the evidence obtained in animal models and clinical studies that posited dopamine receptors as key elements in glucose homeostasis and ultimately led to the FDA approval of bromocriptine in adults with type 2 diabetes to improve glycemic control. Furthermore we discuss the metabolic consequences of treatment with neuroleptics which target the D2R, that should be monitored in psychiatric patients to prevent the development in diabetes, weight gain, and hypertriglyceridemia.","palabras_clave":"DOPAMINE|OBESITY|DIABETES|PSYCHIATRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5239,"idioma_id":4,"titulo":"Transition Metal-free Photoorganocatalytic Fluoroalkylation Reactions of Organic Compounds","resumen":"10.2174\/1385272820666160614080432The generation of perfluoroalkyl radicals (RF?) by means of photocatalysis is a more suitable route than the established methods employing chemical initiators, like azo or peroxide compounds or the use of transition metals. The photocatalytic reaction conditions can tolerate a wide range of functional groups and, therefore, can be applied to the late-stage modification of bioactive molecules. Organic photocatalysts (POC), in the absence of transition metals, can suffer electron-exchange reactions, yielding RF radicals. RF radical production without the assistance of transition metals through the employment of POC will be described, applying this methodology to studying classical examples.","palabras_clave":"photocatalysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5240,"idioma_id":4,"titulo":"Env-specific IgA from Viremic HIV-infected Subjects Compromises Antibody-Dependent Cellular Cytotoxicity","resumen":"Elucidating thefactors that modulate HIV-specific antibody-dependent cellular cytotoxicity(ADCC) will help to understand its role in HIV immunity. The aim of this studywas to determine whether IgA couldmodify ADCC magnitude in HIV infection, abrogatingits protective role. Plasma from 20 HIV+ subjects enrolledduring primary HIV infection (PHI), 10 chronically infected subjects (Chronics)and 7 Elite Controllers (EC) was used. ADCC was determined using a fluorometricADCC assay, before and after plasma IgA removal. Data was analyzed usingnon-parametric statistics. ADCC was documented in 80% of PHI enrollmentsamples and in 100% of PHI 12-month samples, Chronics and ECs; it peaked afteracute infection, reached a plateau in chronic infection and decreased after ARTinitiation. Significant associations between ADCC and disease progression wereonly found after IgA plasma removal from 12-month PHI samples: ADCC magnitudenot only increased after IgA removal but also correlated with CD4+T-cellpreservation. This work provides evidence that gp120-specific IgA was capableof modifying ADCC responses during natural HIV infection for the first time andadds to similar evidence provided in other settings. Furthermore, it underscoresthe complexity of ADCC phenomenon and will help to understand its underlyingmechanisms.  ","palabras_clave":"HIV|ADCC|IgA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5241,"idioma_id":4,"titulo":"Modification of the HIV-specific CD8+ T cell response in an HIV Elite Controller after Chikungunya virus infection","resumen":"Objective: To evaluate the impact of Chikungunya virus (CHIKV) infection on thequality of the HIV-specific CD8+T-cell (CTL) response inan HIV Elite Controller (EC).Design: Three blood samples were obtained from an EC at 27 days (EC-CHIKV,Sample1, S1); 41 days (S2) and one year (S3) after CHIKV infection. Additionally,samples from other nine ECs and nine viremic Chronics were obtained.Methods: CD4+ T-cell counts, viral load (VL) and immune activationwere recorded. NK cells and HIV-specific CTL quality were evaluated. Data wasanalysed using non-parametric statistics.Results: A male HIV EC was confirmed for CHIKV infection. At S1, he presented211 CD4+ T-cells\/&#956;l, a HIV VL blip (145 copies\/ml) and high T-cell activation.NK cell percentage and activation were higher at S2. All parameters wererecovered by S3. CTLs at S1 were exclusively monofunctional with a highproportion (>80%) of degranulating CTLs. By S3, CTL polyfunctionality was more similarto that of typical EC. The distribution of CD8+T-cell memory subsetsalso displayed altered profiles. Conclusions: The results showed that the phenotype and function of HIV-specific CTLs weremodified in temporal association with an HIV VL blip that followed CHIKVinfection. This might have help to control the transient HIV rebound. Additionally,NK cells could have been involved in this control. These results provide usefulinformation to help understand how EC maintain their status, control HIVinfection and alert about the negative impact to the immune function ofHIV-infected subjects living in CHIKV endemic areas.","palabras_clave":"CD8+ T cell response| Chikungunya virus|Elite Controller|HIV"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5242,"idioma_id":4,"titulo":"Ecological processes and biogeochemical cycling in salt marshes: synthesis of studies in the Bahia Blanca estuary (Argentina)","resumen":"Here we offer an integrative review of biogeochemical cycling of carbon, nitrogen, phosphorus,and several metals in salt marshes of the Bahía Blanca Estuary, located in South America, which is a region underrepresented in the literature. The dominant species, Spartina alterniflora and Sarcocornia perennis, have low net aboveground primary productivity but play substantial and contrasting roles in the biogeochemical cycling of elements. S. perennis was more efficient at metal sequestration, whereas S. alterniflora was important in the immobilization of  phosphorus. Because of the differences in net aboveground primary productivity between high and low marsh, plant position should be considered to evaluate the role of S. alterniflora on biogeochemical cycles. Some elements were also in high concentrations in belowground tissues but, based on our data, we could not accurately estimate net belowground primary productivity, a key process to evaluate elemental cycling in salt marshes. In spite of uncertainties in the estimations, the slower decomposition rates in S. alterniflora would be indicative of a higher contribution to the long-term storage of nutrients and metals within the marsh. Regardless shortcomings, our work represents a valuable tool for comparisons with salt marshes worldwide","palabras_clave":"CARBON|NITROGEN|PHOSPHORUS|METALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5243,"idioma_id":4,"titulo":"Long-legged caracaras: terrestrial habitat and hind limb morphology","resumen":"The hindlimb of the Polyborinae has always been characterized as long when itwas compared with other diurnal raptors; however, no empirical work has beendone to support such a traditional well-known fact. The objective of this work is to analyze the femur, tibiotarsus and tarsometatarsus of this group to corroborate whether this assumption has a significant statistical support. The proportions of these bones were analyzed with a one-way Kruskal?Wallis test and Dunn?s test as post hoc comparisons. The allometric relationships between the long bones and body mass or the total length of the hindlimb were carried out with the reduced major axis method. In addition, the osteological study is complemented with a myological dissection of the hindlimb of Milvago chimachima and Caracara plancus.The three long bones of the limb of Polyborinae show significant differenceswith those from Falconinae. The presence of a long tarsometatarsus is a typicalfeature of terrestrial birds, it produces an increase in length stride allowing an effective locomotion on land. Among the myological differences, the most prominent are the presence of the musculus flexor cruris lateralis (absent in other Falconidae) and a well-developed musculus fibularis longus. These osseous and myological features are related to the terrestrial locomotion present in this subfamily","palabras_clave":"Falconidae|osteology|myology|femur"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5244,"idioma_id":4,"titulo":"The effects of trans-fatty acids on TAG regulation in mice depend on dietary unsaturated fatty acids","resumen":"The aim of this study was to investigate the effects of trans-fatty acids (TFA) on liver and serum TAG regulation in mice fed diets containing different proportions of n-3, n-6 and n-9 unsaturated fatty acids (UFA) from olive (O), maize (C) or rapeseed (R) oils partially substituted or not with TFA (Ot, Ct and Rt, respectively). Male CF1 mice were fed (30 d) one of these diets. The effects of the partial substitution (1 %, w\/w) of different UFA with TFA on the activity and expression of hepatic enzymes involved in lipogenesis and fatty acids oxidation were evaluated, as well as their transcription factor expressions. Some of the mechanisms involved in the serum TAG regulation, hepatic VLDL rich in TAG (VLDL-TAG) secretion rate and lipoprotein lipase (LPL) activity were assessed. In liver, TFA induced an increase in TAG content in the Ot and Rt groups, and this effect was associated with an imbalance between lipogenesis and &#946;-oxidation. In the Ot group, exacerbated lipogenesis may be one of the mechanisms responsible for the liver steatosis induced by TFA, whereas in Rt it has been related to a decreased &#946;-oxidation, compared with their respective controls. The enhanced hepatic VLDL-TAG secretion in the Ot and Rt groups was compensated with a differential removal of TAG by LPL enzyme in extrahepatic tissues, leading to unchanged serum TAG levels. In brief, the effects of low levels of TFA on liver and serum TAG regulation in mice depend on the dietary proportions of n-3, n-6 and n-9 UFA.","palabras_clave":"Dietary fat|TAG metabolism|Lipogenesis|b-oxidation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5245,"idioma_id":4,"titulo":"'A highly nutritive bread, developed and gamma irradiated to serve in disaster relief or as an emergency ration''","resumen":"A shelf stable highly nutritive bread (HNB), formulated to cover nutritional requirements of people in need of an emergency staple food, was gamma irradiated to improve its sanitary quality and extend shelf life. Packed in polyethylene film and gamma irradiated at 6 kGy, it attained at least a six times shelf life extension, being stored during 43 days at room temperature while maintaining its sensory quality. This HNB remained microbiologically safe for at least nine months","palabras_clave":"IRRADIATION|BREAD|SHELF LIFE|SENSORY QUALITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5247,"idioma_id":4,"titulo":"Binding of the substrate UDP-glucuronic acid induces conformational changes in the xanthan gum glucuronosyltransferase","resumen":"GumK is a membrane-associated glucuronosyltransferase of Xanthomonas campestris that is involved in xanthan gum biosynthesis. GumK belongs to the inverting GT-B superfamily and catalyzes the transfer of a glucuronic acid (GlcA) residue from uridine diphosphate (UDP)-GlcA (UDP-GlcA) to a lipid-PP-trisaccharide embedded in the membrane of the bacteria. The structure of GumK was previously described in its apo- and UDP-bound forms, with no significant conformational differences being observed. Here, we study the behavior of GumK toward its donor substrate UDP-GlcA. Turbidity measurements revealed that the interaction of GumK with UDP-GlcA produces aggregation of protein molecules under specific conditions. Moreover, limited proteolysis assays demonstrated protection of enzymatic digestion when UDP-GlcA is present, and this protection is promoted by substrate binding. Circular dichroism spectroscopy also revealed changes in the GumK tertiary structure after UDP-GlcA addition. According to the obtained emission fluorescence results, we suggest the possibility of exposure of hydrophobic residues upon UDP-GlcA binding. We present in silico-built models of GumK complexed with UDP-GlcA as well as its analogs UDP-glucose and UDP-galacturonic acid. Through molecular dynamics simulations, we also show that a relative movement between the domains appears to be specific and to be triggered by UDP-GlcA. The results presented here strongly suggest that GumK undergoes a conformational change upon donor substrate binding, likely bringing the two Rossmann fold domains closer together and triggering a change in the N-terminal domain, with consequent generation of the acceptor substrate binding site.","palabras_clave":"conformational change|glycosyltransferase|membrane monotopic protein|xanthan"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5248,"idioma_id":4,"titulo":"Comparative bioaugmentation with a consortium and a single strain in a phenanthrene-contaminated soil: Impact on the bacterial community and biodegradation","resumen":"The efficiency of two inoculation strategies, using a consortium (CON) or an isolated strain (AM), on phenanthrene-contaminated soil was determined with special concern on the study of the bacterial community composition by PCR-DGGE and pyrosequencing of 16S rRNA gene fragments. Both strategies stimulated the phenanthrene degradation, increasing the cultivable heterotrophic bacteria number and biological activity. At the end of the treatments, the microcosms inoculated with AM reached the lowest values of phenanthrene but also the lowest dehydrogenase activity. In DGGE patterns a reduction in number of bands in the contaminated and inoculated microcosms was observed, being the most significant differences attributed to inoculation with AM. The pyrosequencing technique yielded results that correlated with the fingerprint, showing that the bacterial community composition based on relative abundance was significantly modified by treatments. Sphingomonadales and Burkholderiales were highly stimulated by phenanthrene contamination and inoculation. In the Phe microcosm, the higher increase in Actinomycetales (mainly Arthrobacter) was observed. Effectively, the use of the strain AM as inoculant became the best strategy to remediate the soil mainly based on the degradation efficiency, however it caused more drastic changes in microbial community than inoculation with CON, what can be compromising the ulterior functionality of the soil.","palabras_clave":"Bioaugmentation|Bacterial consortium|Community composition|Pyrosequencing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5249,"idioma_id":4,"titulo":"Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing","resumen":"The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on Actinomycetales and Sphingomonadales orders, while inoculation caused a relative decline of Actinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of Sphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between those genera, our results suggest that this would not be directly related to PAH degradation.","palabras_clave":"Bioaugmentation|Sphingobium sp. AM genome|Soil microbiome|Inoculant establishment|Community diversity|Pyrosequencing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5250,"idioma_id":4,"titulo":"Application of the WRF-LETKF Data Assimilation System over Southern South  America: Sensitivity to model physics","resumen":"Improving the initial conditions of short-range numerical weather prediction (NWP) models is one of the main goals of the meteorological community. Development of data assimilation and ensemble forecast systems is essential in any national weather service (NWS). In this sense, the local ensemble transform Kalman filter (LETKF) is a methodology that can satisfy both requirements in an efficient manner. The Weather Research and Forecasting (WRF) Model coupled with the LETKF, developed at the University of Maryland, College Park, have been implemented experimentally at the NWS of Argentina [Servicio Meteorológico Nacional (SMN)], but at a somewhat lower resolution (40 km) than the operational Global Forecast System (GFS) at that time (27 km). The purpose of this work is not to show that the system presented herein is better than the higher-resolution GFS, but that its performance is reasonably comparable, and to provide the basis for a continued improved development of an independent regional data assimilation and forecasting system. The WRF-LETKF system is tested during the spring of 2012, using the prepared or quality controlled data in Binary Universal Form for Representation of Meteorological Data (PREPBUFR) observations from the National Centers for Environmental Prediction (NCEP) and lateral boundary conditions from the GFS. To assess the effect of model error, a single-model LETKF system (LETKF-single) is compared with a multischeme implementation (LETKF-multi), which uses different boundary layer and cumulus convection schemes for the generation of the ensemble of forecasts. The performance of both experiments during the test period shows that the LETKF-multi usually outperforms the LETKF-single, evidencing the advantages of the use of the multischeme approach. Both data assimilation systems are slightly worse than the GFS in terms of the synoptic environment representation, as could be expected given their lower resolution. Results from a case study of a strong convective system suggest that the LETKF-multi improves the location of the most intense area of precipitation with respect to the LETKF-single, although both systems show an underestimation of the total accumulated precipitation. These preliminary results encourage continuing the development of an operational data assimilation system based on WRF-LETKF at the SMN.","palabras_clave":"MATHEMATICAL AND STATISTICAL TECHNIQUES|KALMAN FILTERS|FORECASTING|NUMERICAL WEATHER PREDICTION\/FORECASTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5252,"idioma_id":4,"titulo":"Genomics of high molecular weight plasmids isolated from an on-farm biopurification system","resumen":"The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.","palabras_clave":"biopurification systems |plasmids"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5253,"idioma_id":4,"titulo":"Effect of the addition of phytosterols and tocopherols on Streptococcus thermophilus robustness during industrial manufacture and ripening of a functional cheese as evaluated by qPCR and RT-qPCR","resumen":"The quality of functional food products designed for the prevention of degenerative diseases can be affected by the incorporation of bioactive compounds. In many types of cheese, the performance of starter microorganisms is critical for optimal elaboration and for providing potential probiotic benefits. Phytosterols are plant lipophilic triterpenes that have been used for the design of functional dairy products because of their ability to lower serum cholesterol levels in humans. However, their effect on the starter culture behavior during cheesemaking has not yet been studied. Here, we followed DNA and RNA kinetics of the bacterium Streptococcus thermophilus, an extensively used dairy starter with probiotic potential, during industrial production of a functional, semi-soft, reduced-fat cheese containing phytosterol esters and alpha-tocopherol as bioactive compounds. For this purpose, real-time quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) assays were optimized and applied to samples obtained during the manufacture and ripening of functional and control cheeses. An experimental set-up was used to evaluate the detection threshold of free nucleic acids for extraction protocols based on pelleted microorganisms. To our knowledge, this straight-forward approach provides the first experimental evidence indicating that DNA is not a reliable marker of cell integrity, whereas RNA may constitute a more accurate molecular signature to estimate both bacterial viability and metabolic activity. Compositional analysis revealed that the bioactive molecules were effectively incorporated into the cheese matrix, at levels considered optimal to exert their biological action. The starter S. thermophilus was detected by qPCR and RT-qPCR during cheese production at the industrial level, from at least 30 min after its inoculation until 81 days of ripening, supporting the possible role of this species in shaping organoleptic profiles. We also showed for the first time that the addition of phytosterols at functional concentrations, not only did not affect starter performance but also correlated with a significant increase in target DNA and cDNA levels in most of the time points evaluated throughout cheesemaking. Therefore, these findings suggest that the growth and metabolism of S. thermophilus may be enhanced by the incorporation of these biologically active molecules during cheese production, providing important information for the industrial design of novel fermented foods.","palabras_clave":"Cheese|Starter|Lab|PCR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5254,"idioma_id":4,"titulo":"Enzymatic tandem systems engineered from mesoporous thin films: Synergy leading to efficient starch-electricity conversion","resumen":"The synergetic combination of nanomaterials with biomolecules, which is inherent to natural systems is a challenging and thriving field. In Nature, a wide variety of enzymes work together in multi-step reactions into sophisticated subcellular compartments. Mesoporous Oxide Thin Films (MOTF) permit to divide the space in highly ordered functional domains, providing a promising multiscale scaffold to host enzymes. However, the emergent properties of MOTF in which enzymes are hierarchically ordered and coupled together have not been explored yet. Here, we demonstrate how the confined cavities of an organized multilayer of MOTF, in resemblance to living cell compartments, can be used to host two different enzymes to activate a cascade of enzyme reactions that do not proceed under free enzyme conditions. Transformation of complex carbohydrates such as starch to electricity is widely recognized to be an important technological objective. The MOTF multilayer was used as a bionanofactory, achieving a starch-fuelled biofuel cell that yields the best performance obtained so far, jointly with reduced operating fuel requirement. This development demonstrates that enzymatic pathways can be engineered from mesoporous thin film architectures, opening the way for evolving bio-active surfaces in packaging, sensors, prosthetics, or bioMEMS among other exciting applications.","palabras_clave":"MESOPOROUS MATERIALS|ENZYMATIC TANDEM|BIOFUEL CELLS|THIN FILMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5255,"idioma_id":4,"titulo":"Antimicrobial Surfaces from Incorporated Nano-agents","resumen":"Biofilm development is a survival strategy for majority of bacteria to adapt to their living environment. These biofilms are prevalent on most surfaces in nature. Microbial cells in biofilm exhibit significantly enhanced tolerance and resistance to antimicrobial challenge and host defenses. Therefore, the control and eradication of biofilm-associated diseases present a great challenge. Recently, there is considerable biomedical incentive in the development of nanoparticles with self-antibacterial activity or as antibiotic carriers. The advantages of coatings incorporating different types of nano-agents for antimicrobial surface generation are here reviewed. Although the main coatings currently used or investigated and the associated synthesis processes are individually described, emphasis ismade on silver-loaded mesoporous thin coatings. Recent developments suggest that mesoporous oxide thin films (especially titania) incorporating metal nanoparticles is becoming a prime candidate for antimicrobial coatings.","palabras_clave":"Antimicrobial coating|Self-antibacterial nanoparticles|Antibiotic carriers|Nanocomposite|Mesoporous films|Biofilms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5256,"idioma_id":4,"titulo":"Influence of Incorporation of Starch Nanoparticles in PBAT\/TPS Films","resumen":"Ver adjunto","palabras_clave":"PBAT|TPS|starch nanoparticles|thermal properties|biodegradability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5257,"idioma_id":4,"titulo":"Data of thermal degradation and dynamic mechanical properties of starch-glycerol based films with citric acid as crosslinking agent","resumen":"Interest in biodegradable edible films as packaging or coating hasincreased because their beneficial effects on foods.In particular,food products are highly dependents on thermal stability,integrityand transition process temperatures of the packaging.The presentwork describes a complete data of the thermal degradation anddynamic mechanical properties of starch?glycerol based films withcitric acid(CA) as crosslinking agent described in the article titled:?Biodegradable and non-retrogradable eco-films based on starch?glycerol with citric acid as crosslinking agent? González Seligraet al.(2016)[1].Data describes thermogravimetric and dynamicalmechanical experiences and provides the figures of weight lossand loss tangent of the films as a function of the temperature.","palabras_clave":"Starch|Citric acid|Biodegradable edible films|Thermal degradation|Dynamic mechanical propieties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5258,"idioma_id":4,"titulo":"Distribution of bovine herpesvirus type 1 in the nervous system of experimentally infected calves","resumen":"Bovine herpesvirus type 1 (BoHV-1) is responsible for respiratory and genital disease in cattle. BoHV-1 encephalitis is only occasionally reported. However, several cases of neurological disease have been recently attributed to BoHV-1. In this study, the distribution and pathological alterations caused by two BoHV-1 strains in the nervous system of experimentally infected calves during acute infection and reactivation are described. Calves were inoculated intranasally with BoHV-1 Los Angeles (BoHV-1.LA) or Cooper (BoHV-1.Cooper) strains. Acutely infected calves were euthanased at 6 days (BoHV-1.Cooper, n&#8201;=&#8201;2) and 7 days post-inoculation (BoHV-1.LA, n&#8201;=&#8201;2). Latently infected calves that were given dexamethasone to induce reactivation were euthanased at 2 days (BoHV-1.Cooper, n&#8201;=&#8201;2) or 5 days (BoHV-1.LA, n&#8201;=&#8201;2) after dexamethasone administration. Both BoHV-1 strains were isolated from the brains of acutely infected calves. Distribution of viral DNA in the neural tissues was similar for both strains. During reactivation, neither BoHV-1.LA nor BoHV-1.Cooper was isolated from any brain section or trigeminal ganglia in infected calves. Macroscopic lesions were not evident in any group. In BoHV-1.LA infected calves, microscopic lesions were found in the brain but not in the trigeminal ganglia. Microscopic lesions in the brain of BoHV-1.Cooper infected calves were not as evident as in BoHV-1.LA infected animals. However, mononuclear infiltrates and neuronophagia were present in trigeminal ganglia. The results of this study demonstrated that respiratory BoHV-1 strains are able to replicate and disseminate within the bovine nervous tissue and provide evidence of the neuroinvasiveness of BoHV-1 strains.","palabras_clave":"BOVINE HERPESVIRUS TYPE 1|NEUROINVASIVENESS|ENCEPHALITIS|MICROSCOPIC LESIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5259,"idioma_id":5,"titulo":"?Tratamiento de reagudizaciones post terapia endodóntica?","resumen":"RESUMENDespués del tratamiento del conducto radicular puede presentarse un leve dolor incluso cuando el tratamiento se realizó bajo estándares clínicos adecuados, sin embargo, durante la terapia, algunos pacientes pueden experimentar una exacerbación , esta se expresa con síntomas clínicos como dolor espontáneo,a la masticación e hinchazón de los tejidos blandos de cara y mucosa oral en el área del diente tratado, y requiere atención odontológica inmediata. Las exacerbaciones son descriptas como un fenómeno polietiológico. El propósito deeste trabajo fue mostrar la frecuencia con que ocurren las reagudizaciones,evaluar los factores que intervienen en su aparición y determinar las distintas alternativas de tratamiento. Se presentó un caso clínico de un paciente al que se le realizaron 5 tratamientos endodónticos con diferentes diagnósticos,una sola sesión operatoria. Luego de realizados los tratamientos, se produjo una reagudización en el elemento 14. Se optó como tratamiento la terapia antibiótica. Los factores etiológicos en ocasiones no se pueden determinar con precisión, muchos de los tratamientos son en función de la prevención o del alivio de los síntomas durante la terapia endodóntica.ABSTRACTAfter root canal treatment there may be a slight pain even when the treatment was carried out under appropriate clinical standards, however, during therapy,some patients may experience an exacerbation, this is expressed with clinical symptoms such as spontaneous pain, chewing and swelling of the soft tissues of the face and oral mucosa in the area of the treated tooth, and requires immediate dental care. Exacerbations are described as a phenomenon polietiológico. The purpose of this study was to show the frequency of occurrence of exacerbations, evaluate the factors involved in its appearance and determine the various treatment alternatives. A case of a patient who performed five endodontic treatments with different diagnoses, a single operative session was presented. After the treatments performed, an exacerbation occurred in the element 14. antibiotic therapy was chosen as a treatment. Etiologicalfactors sometimes can not be determined accurately, many treatments are based on the prevention or relief of symptoms during endodontic therapy.","palabras_clave":"TRATAMIENTO ENDODÓNTICO|FLARE UP|EXCACERVACION AGUDA|DOLOR POSTOPERATORIO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5260,"idioma_id":4,"titulo":"Molecular evidence of hybrid zones of Cedrela (Meliaceae) in the Yungas of Northwestern Argentina","resumen":"In the Yungas of Northwestern Argentina, three endangered species of Cedrela (C. angustifolia, C. saltensis,and C. balansae) follow altitudinal gradients of distribution with contact zones between them. We sampled210 individuals from 20 populations that spanned most of Cedrela?s geographical range in theYungas, and used Amplified Fragment Length Polymorphism (AFLP) markers and DNA sequences of thenuclear Internal Transcribed Spacer (ITS) to investigate hybrid zones. Data analyses employed an arrayof complementary methods, including principal coordinate analyses, Bayesian clustering analyses, maximumlikelihood tree-building, and network techniques. Both nuclear molecular systems ? AFLP and ITS ?provided insights into the evolutionary history of Cedrela in the Yungas in a congruent manner. Weuncovered strong support for the occurrence of natural hybridization between C. balansae and C. saltensis.Additionally, we identified hybrid zones in areas of sympatry (at both the Calilegua National Park and theSan Andrés farm) and in transition zones from 820 to 1100 meters above sea level (localities of Pintascayoand Acambuco). There was no evidence for hybridization of either C. balansae or C. saltensis with C. angustifolia.The role of hybrid populations in conservation and use of genetic resources in the Yungas werediscussed.","palabras_clave":"Amplified Fragment Length Polymorphism (AFLP) |Cedrela |Genetic structure Hybrid zones |Internal Transcribed Spacer (ITS)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5261,"idioma_id":5,"titulo":"Reparación apical posterior al tratamiento endodóntico","resumen":"RESUMENEl proceso de cicatrización involucra una serie de eventos que ocurren en respuesta a una lesión producida por agentes injuriantes. Su finalidad es lograr que ese tejido dañado sane.El objetivo de este trabajo fue describir cada una de las fases de la reparación, así como también los sistemas celulares pertenecientes a los tejidos involucrados en el proceso reparativo postendodoncia. Al conocer íntimamente estos mecanismos, se logrará analizar la variabilidad de respuesta del organismo para poder así predecir las posibilidades de éxito o fracaso en la terapia endodóntica. A partir de criterios histológicos, radiológicos y clínicos se podrá evaluar la respuesta biológica de cada paciente.ABSTRACTThe healing process involves a series of events that occur in response to injury caused by harmful agents. It seeks to heal the damaged tissue. The aim of this study was to describe each of the stages of repair, as well as cellular systems belonging to the tissues involved in the repair process postendodoncia. To intimately understand these mechanisms, it will be possible to analyze thevariability of response of the body to well predict the chances of success or failure in endodontic therapy. From histological, radiological and clinical criteria can evaluate the biological response of each patient.","palabras_clave":"CIERRE APICAL|HISTOLOGÍA|REPARACIÓN APICAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5263,"idioma_id":4,"titulo":"Potent inhibition of Western Equine Encephalitis virus by a fraction rich in flavonoids and phenolic acids obtained from Achyrocline satureioides","resumen":"Achyrocline satureioides (Lam.) DC. Asteraceae, ?marcela del campo?, possess several pharmacological properties. Previously we reported antiviral activity of an aqueous extract of A. satureioides against an alphavirus, Western Equine Encephalitis virus. Alphaviruses are highly virulent pathogens which cause encephalitis in humans and equines. There are no effective antiviral to treat its infections. The aim of this study was to evaluate in vitro cytotoxic and antiviral activities against Western Equine Encephalitis virus of five water extract chromatographic fractions from A. satureioides and identify the main compounds of the bioactive fraction. Also, it was to assess in vivo cytogenotoxic ability of the active fraction. Cytotoxicity studies revealed low toxicity of the most of fractions in Vero and in equine peripheral blood mononuclear cells. Antiviral studies showed that the water crude extract-Sephadex LH 20-fraction 3 MeOH-H2O (Fraction 3) was active against Western Equine Encephalitis virus with Effective Concentration 50% = 5µ g\/ml. Selectivity Indices were 126.0 on Vero and 133.6 on peripheral blood mononuclear cells, four times higher than aqueous extract selectivity index. Regarding the mechanism of action we demonstrated that F3 exerted its action in intracellular replication stages. Further, fraction 3 showed important virucidal action. Fraction 3 contains, in order of highest to lowest: chlorogenic acid, luteolin, 5,7,8-trimethoxyflavone, 3-O-methylquercetin and caffeic acid. Fraction 3 did not induce in vivo toxic nor mutagenic effect. Therefore, it is safe its application as antiviral potential. Further studies of antiviral activity in vivo will be developed using a murine model.","palabras_clave":"ACHYROCLINE SATUREIOIDES|ANTIVIRAL ACTIVITY|CYTOTOXICITY|GENOTOXICITY|WESTERN EQUINE ENCEPHALITIS VIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5264,"idioma_id":4,"titulo":"The antitripanocide benznidazole promotes adaptive response to oxidative injury: Involvement of the nuclear factor-erythroid 2-related factor-2 (Nrf2) and multidrug resistance associated protein 2 (MRP2)","resumen":"Oxidative stress is a frequent cause underlying drug-induced hepatotoxicity. Benznidazole (BZL) is the only antitripanocide agent available for treatment of Chagas disease in endemic areas. Its use is associated with side effects, including increases in biomarkers of hepatotoxicity. However, BZL potential to cause oxidative stress has been poorly investigated. Here, we evaluated the effect of a pharmacologically relevant BZL concentration (200&#956;M) at different time points on redox status and the counteracting mechanisms in the human hepatic cell line HepG2. BZL increased reactive oxygen species (ROS) after 1 and 3h of exposure, returning to normality at 24h. Additionally, BZL increased glutathione peroxidase activity at 12h and the oxidized glutathione\/total glutathione (GSSG\/GSSG+GSH) ratio that reached a peak at 24h. Thus, an enhanced detoxification of peroxide and GSSG formation could account for ROS normalization. GSSG\/GSSG+GSH returned to control values at 48h. Expression of the multidrug resistance-associated protein 2 (MRP2) and GSSG efflux via MRP2 were induced by BZL at 24 and 48h, explaining normalization of GSSG\/GSSG+GSH. BZL activated the nuclear erythroid 2-related factor 2 (Nrf2), already shown to modulate MRP2 expression in response to oxidative stress. Nrf2 participation was confirmed using Nrf2-knockout mice in which MRP2 mRNA expression was not affected by BZL. In summary, we demonstrated a ROS increase by BZL in HepG2 cells and a glutathione peroxidase- and MRP2 driven counteracting mechanism, being Nrf2 a key modulator of this response. Our results could explain hepatic alterations associated with BZL therapy.","palabras_clave":"BENZNIDAZOLE|MRP2|NRF2|HÍGADO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5265,"idioma_id":4,"titulo":"Toll-like receptors, IFN-&#947; and IL-12 expression in bovine leukemia virus-infected animals with low or high proviral load","resumen":"Bovine leukemia virus (BLV) infection iswidespread mainly in dairy cattle and 5?10% of infected animalswill die due to lymphosarcoma; most cattle remain asymptomatic but 30% develop persistent lymphocytosis (PL). BLV transmission depends on infected cell exchange and thus, proviral load is determinant. Understanding the mechanismswhich govern the control of viral disseminationwill be desirable for the design of effective therapeutic or preventive strategies for BLV. The development of high proviral load (HPL) or low proviral load (LPL) might be associated to genetic factors and humoral immune responses, however cellular responses are not fully described. We aimed to characterize cytokines and toll-like receptors (TLR) expression related to the proviral load profiles. IFN-&#947; and IL-12 mRNA expression levelwas significantly higher in PBMC frominfected cattle (LPL n=6 and HPL n=7) compared to uninfected animals (n=5). While no significant differenceswere observed in IL-12 expression between LPL and HPL group, IFN-&#947; expression was significantly higher in LPL animals. Infected cattle exhibited higher expression levels of TLR3, 7?9. Animals with HPL had significantly higher expression of TLR7\/8 than uninfected cattle. TLR8 and TLR9 were up-regulated in HPL group, and TLR3 was up-regulated in LPL group. This is the first report related to TLR gene expression in BLV infected cattle and represents evidence of the involvement of these receptors in BLV recognition. Further studies on different subpopulations of immune cells may help clarify their role in response to BLV and its consequences on viral dissemination.","palabras_clave":"BOVINE LEUKEMIA VIRUS|PROVIRAL LOAD|TOLL-LIKE RECEPTORS|IFN-&#947;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5266,"idioma_id":4,"titulo":"?REDUCTION OF CANDIDA TROPICALIS BIOFILM BY PHOTOACTIVATION OF A HETEROPHYLLAEA  PUSTULATA EXTRACT","resumen":"Context: Biofilm formation is an important problem, since this growth mode confers resistance to drugs usually used in therapeutics. Objective: In vitro antifungal activity of extracts obtained from Heterophyllaea pustulata Hook f. (Rubiaceae) were studied against Candida tropicalis biofilms, evaluating the effect of irradiation and the oxidative and nitrosative stresses as possible mechanisms of action. Materials and methods: Hexane, benzene, ethyl acetate and ethanol extracts were evaluated at three concentrations (0.2, 0.1 and 0.05mg\/mL) over mature biofilm, under darkness and irradiation. After 48 h of incubation, biofilm quantitation was performed by the O?Toole and Kolter method. Reactive oxygen species (ROS) was measured by nitro-blue tetrazolium (NBT) reaction and reactive nitrogen intermediates (RNI) by the Griess reagent. Superoxide dismutase activation (SOD, NBT assay) and total antioxidant system (FRAP test) were studied. Results: Only the benzene extract at 0.2mg\/mL reduced the biofilms formation. The slight decrease achieved in darkness (17.06 ± 2.80% reduction) was increased by light action (39.31 ± 3.50% reduction), clearly observing a photostimulation. This great reduction was confirmed by confocal microscopy. In darkness, biofilm reduction was mediated by an increase in RNI, whereas under irradiation, the ROS action was most important. Although no SOD activation was observed, a strong stimulation of the total antioxidant system was detected. HPLC analysis established a high content of several anthraquinones in this extract. Discussion and conclusion: Biofilm reduction by benzene extract was mainly mediated by oxidative stress triggered under light action, confirming a photodynamic sensitization, which could be attributed to its high content of photosensitizing anthraquinones","palabras_clave":"CANDIDA BIOFILMS|HPLC ANALYSIS|PHOTOSENSITIZATION|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5267,"idioma_id":4,"titulo":"Cathelicidin Enhances the LPS Inducing Synthesis of Toll-like Receptors 4 inthe Colonic Epithelium","resumen":"Cathelicidin Enhances the LPS Inducing Synthesis of Toll-like Receptors 4 inthe Colonic Epithelium","palabras_clave":"INNATE IMMUNE RESPONCE|CATHELICIDIN|TOLL LIKE RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5268,"idioma_id":4,"titulo":"Phylogeographic analysis of the 2000 2002 foot-and-mouth disease epidemic in Argentina","resumen":"Phylogeographic analysis of the 2000-2002 foot-and-mouth disease epidemic in Argentina","palabras_clave":"Foot-and-mouth disease|Viral transmission|Molecular epidemiology|Phylogeography"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5269,"idioma_id":4,"titulo":"Dendritic cell maturation and cross-presentation: timing matters!","resumen":"As a population, dendritic cells (DCs) appear to be the best cross-presenters of internalized antigens on major histocompatibility complex class I molecules in the mouse. To do this, DCs have developed a number of unique and dedicated means to control their endocytic and phagocytic pathways: among them, the capacity to limit acidification of their phagosomes, to prevent proteolytic degradation, to delay fusion of phagosomes to lysosomes, to recruit ER proteins to phagosomes, and to export phagocytosed antigens to the cytosol. The regulation of phagocytic functions, and thereby of antigen processing and presentation by innate signaling, represents a critical level of integration of adaptive and innate immune responses. Understanding how innate signals control antigen cross-presentation is critical to define effective vaccination strategies for CD8(+) T-cell responses.","palabras_clave":"CROSS-PRESENTATION|DENDRITIC CELL|DENDRITIC CELL MATURATION|PHAGOCYTOSIS|TOLL-LIKE RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5270,"idioma_id":4,"titulo":"Data supporting the cardiac mitochondria calcium handling in female normotensive and spontaneously hypertensive rats","resumen":"In associated with the published article ¨Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels¨ in Mitochondrion (Pardo et al., 2015) [1], this data article contains information about calcium handling of cardiac mitochondria isolated from female of both rats strains (WKY and SHR). Dataset of mitochondrial permeability transition pore (mPTP) resistance to opening Ca2+-mediated, Ca2+ retention capacity (CRC), time constants and mitochondrial membrane potential (delta&#936;m) are showed.","palabras_clave":"MITOCHONDRIA|CALCIUM|FEMALE|WKY, SHR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5271,"idioma_id":4,"titulo":"Cyclosporine-A mimicked the ischemic pre and postconditioning-mediated cardioprotection in hypertensive rats: role of PKCepsilon","resumen":"Our aim was to assess the action of Cyclosporine-A (CsA) against reperfusion injury in spontaneously hypertensive rats (SHR) compared to the effects of ischemic pre (IP)- and postconditioning (IPC),examining the role played by PKCepsilon.  Isolated hearts were submitted to the following protocols: IC: 45 min global ischemia (GI) and 1 hour reperfusion (R); IP: a cycle of 5 min GI and 10 minutes of R prior to 45 min-GI; IPC: three cycles of 30 sec-GI\/ 30 sec-R at the start of R. Other hearts of IC, IP and IPC groups received CsA (mitochondrial permeability transition pore inhibitor) or chelerythrine (Che, non-selective PKC inhibitor). Infarct size (IS) was assessed. TBARS and reduced glutathione (GSH) content- as parameters of oxidative damage-, the expression of P-Akt, P-GSK-3&#946;, P-PKCepsilon and cytochrome c (Cyc) release- as an index of mitochondrial permeability- and the response of isolated mitochondria to Ca2+ were also measured. IS similarly decreased in preconditioned, postconditioned and CsA treated heart showing the highest values in the combinations IP+CsA and IPC + CsA. TBARS decreased and GSH was partially preserved after all interventions. The content of P-Akt, P-GSK-3&#946; and P-PKCepsilon increased in cytosol and decreased in mitochondria after IP and IPC. In CsA treated hearts these enzymes increased in both fractions reaching the highest values. Cyc release was attenuated and the response of mitochondria to Ca2+ was improved by the interventions. The beneficial effects of IP and IPC were annulled when PKC was inhibited with Che. A PKCepsilon\/VDAC association was also detected. These data show that, in SHR, the CsA treatment mimicked and reinforced the cardioprotective action afforded by IP and IPC in which PKCepsilon- mediated attenuation of mitochondrial permeability appears as the main mechanism involved.","palabras_clave":"CYCLOSPORINE A|SHR|ISCHEMIC PRECONDITIONING|ISCHEMIC POSTCONDITIONING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5272,"idioma_id":4,"titulo":"Ex vivo treatment with a polyphenol-enriched cocoa extract ameliorates myocardial infarct and postischemic mitochondrial injury in normotensive and hypertensive rats","resumen":"Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3&#946;, and P-eNOS were assessed. In isolated mitochondria, the Ca2+-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (&#916;&#968;m), and superoxide production were determined. PCE decreased infarct size, partly preserved GSH, increased the P-Akt, P-GSK-3&#946;, and P-eNOS contents, improved mPTP response to Ca2+, decreased the superoxide production, and restored &#916;&#968;m. These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest that Akt\/GSK-3&#946;\/eNOS dependent pathways are involved.","palabras_clave":"infarct size|mitochondria|polyphenols"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5273,"idioma_id":5,"titulo":"'Los recursos humanos locales como personal de expedición en el mercado de cruceros antárticos'.","resumen":"El trabajo tiene por objetivo conocer la inserción de los recursos humanos locales (residentes en Ushuaia y Argentina) como miembros del staff de los cruceros de expedición de turismo antártico. Para tal fin se llevó adelante un análisis de los despachos de los buques con el fin de identificar los recursos humanos locales involucrados, a quienes se les solicitó que contestaran un cuestionario vía electrónica y, por último, se realizaron entrevistas a actores clave vinculados con el reclutamiento del staff. Los resultados demuestran una importante participación de los recursos humanos locales en el mercado del turismo antártico y permiten conocer los principales factores que favorecen y que también afectan dicha inserción, ayudando a pensar estrategias para logar mayores posibilidades laborales en el futuro.","palabras_clave":"Recursos humanos|staff|cruceros antárticos de expedición|turismo antártico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5274,"idioma_id":4,"titulo":"Uterine blood flow evaluation in bitches suffering from cystic endometrial hyperplasia (CEH) and CEH-pyometra complex","resumen":"Uterine blood flow evaluation in bitches suffering fromcystic endometrial hyperplasia (CEH) and CEH-pyometracomplexP.R. Batista a,b,*, C. Gobello b,c, A. Rubea, Y.A. Corrada b,d, M. Tórtora a,P.G. Blanco a,b,ca Cardiology Laboratory, Clinics Department, Faculty of Veterinary Sciences, National University of La Plata (FVS-NULP), La Plata,Argentinab CONICET, Argentinac Laboratory of Reproductive Physiology, Basic Sciences Department, FVS-NULP, La Plata, Argentinad Teaching Hospital, Clinics Department, FVS-NULP, La Plata, Argentinaa r t i c l e i n f oArticle history:Received 24 May 2015Received in revised form 17 October 2015Accepted 14 December 2015Keywords:Cystic endometrial hyperplasiaPyometraDoppler ultrasoundCaninea b s t r a c tDoppler ultrasound is a useful diagnosis tool to evaluate uterine blood flow in differentcanine reproductive states. The aim of this study was to describe and compare uterineblood flow in bitches suffering from cystic endometrial hyperplasia (CEH) and CEHpyometracomplex (CEH-P). Ninety diestrous bitches were clinically, hematologically,and ultrasonographically classified into four groups: (1) clinical signs, leukocytosis, CEH,and uterine luminal contents (CEH-P, n ¼ 31); (2) asymptomatic bitches with CEH anduterine contents (CEH-C, n ¼ 15); (3) asymptomatic bitches with CEH without uterinecontents (CEH, n ¼ 16); and (4) normal diestrous bitches (ND, n ¼ 28). The widest crosssectionaldiameter and uterine wall thickness (W) of uterine horns were measured usingtwo-dimensional ultrasound. Peak systolic velocity and end diastolic velocity of uterinearteries were measured by Doppler ultrasound in all the bitches. Peak systolic velocity andend diastolic velocity were higher in CEH-P than in CEH-C, CEH, and ND (P  0.1). Conversely, resistance index (RI) reported lower values in CEH-P than that in theother three groups (P  0.1). When all the bitches were considered, correlationbetween RI and cross-sectional diameter was r ¼ 0.69 (P  0.1). It is concluded that the uterine arteryblood flow velocity of bitches suffering from pyometra was higher, not only from normalbitches, but also from females with endometrial hyperplasia. Furthermore, endometrialhyperplasia, accompanied or not by luminal contents, had a higher blood flow velocitythan normal uterus. Hemodynamic parameters appear as useful markers to differentiateuterine pathologic conditions. 2016 Elsevier Inc. All rights reserved.1. IntroductionThe cystic endometrial hyperplasia-pyometra complex(CEH-P) is one of the most prevalent uterine diseases in thefemale dog [1]. Middle-aged to old, diestrous bitches arefrequently affected [1]. Cystic endometrial hyperplasia is a* Corresponding author. Tel.: þ54 221 423 6663; fax: þ54 221 4277378.E-mail address: pbatista@fcv.unlp.edu.ar (P.R. Batista).Contents lists available at ScienceDirectTheriogenologyjournal homepage: www.theriojournal.com0093-691X\/$ ? see front matter  2016 Elsevier Inc. All rights reserved.http:\/\/dx.doi.org\/10.1016\/j.theriogenology.2015.12.008Theriogenology 85 (2016) 1258?1261","palabras_clave":"UTERINE|BLOOD|FLOW|BITCHES|CYSTIC|ENDOMETRIAL|HYPERPLASIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5275,"idioma_id":4,"titulo":"Tetrapod Tracks Taphonomy in Eolian Facies from the Permian of Argentina","resumen":"Ancient desert deposits preserve a copious ichnofossil record, particularly Permian-age deposits wherethe record of tetrapod footprints is present and abundant in almost all desert settings. We propose to analyze, from ataphonomic perspective, Permian footprints preserved in eolian deposits from Argentina with a detailed sedimentological study of the trackway-bearing levels, in order to find evidence of processes that may have enhanced their preservation. We defined four taphonomic modes based on preservation quality, and the morphological and extra-morphological features of the footprints.Mode 1 includes footprints with detailed impressions of the palm, digits and claws. Mode 2 includes tracks with palm and digit impressions associated with small bulbous-shape marginal rims. Mode 3 includes tracks characterized by large, bulbous, marginal rims and randomly preserved palm impressions. Mode 4 includes footprints with shallow digit and palm impressions associated with sand-crescentmarginal rims. The Los Reyunos footprints suggest preservation in: (1) dry sand, evidenced by sediment slipping down-slope structures and (2) subsurface damp sand, evidenced by digit impressions and claw drag traces. Also, we found vertical water content variations along the dune foresets, evidenced by a varying amount of sediment slipping down-slope in the same trackway. Moreover, differences in the time of entombment are suggested by the morphology of rims (bulbous-shape or sand-crescent). The stratigraphic genetic framework resulting from the Los Reyunos taphonomic analysis supports changes in the interstitial subsurface water and rapid entombment of the tracking surface due to a high rate of sediment supply as the main factor for footprint preservation.","palabras_clave":"Taphonomy|Eolian environment|Tetrapod footprints|Permian"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5276,"idioma_id":4,"titulo":"A green-analytical chemistry method for agrochemical-residue analysis in vegetables","resumen":"Tenagrochemicals, including fungicides, insecticides, herbicides and a plant growth regulator, were quantifiedat part per billion levels in complex matrices using a green-analyticalchemistry (GAC) method. Liquid chromatography with dual UV\/diode array (DAD)and fluorescence (FLD) detections was carried out in a single run, and thesecond-order DAD-elution time and FLD-elution time data obtained were treatedwith MCR-ALS (multivariate curve resolution\/alternating least-squares)algorithm. In this way, while analytes are measured through their more appropriate (absorbance and\/orfluorescence) signals, chemometric treatment of the corresponding matricesallows the resolution oftotal or partial overlapped bands, and to overcome the presence ofinterferences in real samples. In this work, FLD-elution time second-order datawere obtained for the first time at two excitation wavelengths, improving thesensitivity of fluorescent analytes. The approach was successfully applied to in land cultivated vegetables, includingmushroom, lettuce, alfalfasprout, cucumber, and celery.","palabras_clave":"UV FLUORESCENCE LIQUID CHROMATOGRAPHY|SECOND ORDER CALIBRATION|AGROCHEMICALS|VEGETABLE SAMPLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5277,"idioma_id":4,"titulo":"Enantiomeric analysis of overlapped chromatographic profiles in thepresence of interferences. Determination of ibuprofen in apharmaceutical formulation containing homatropine","resumen":"In this work, we studied the combination of chemometric methods with chromatographic separations as astrategy applied to the analysis of enantiomers when complete enantioseparation is difficult or requireslong analysis times and, in addition, the target signals have interference from the matrix. We presentthe determination of ibuprofen enantiomers in pharmaceutical formulations containing homatropineas interference by chiral HPLC-DAD detection in combination with partial least-squares algorithms. Themethod has been applied to samples containing enantiomeric ratios from 95:5 to 99.5:0.5 and coelutionof interferents. The results were validated using univariate calibration and without homatropine. Relativeerror of the method was less than 4.0%, for both enantiomers. Limits of detection (LOD) and quantifica-tion (LOQ) for (S)-(+)-ibuprofen were 4.96 × 10&#8722;10and 1.50 × 10&#8722;9mol, respectively. LOD and LOQ forthe R-(&#8722;)-ibuprofen were LOD = 1.60 × 10&#8722;11mol and LOQ = 4.85 × 10&#8722;11mol, respectively. Finally, thechemometric method was applied to the determination of enantiomeric purity of commercial pharma-ceuticals. The ultimate goal of this research was the development of rapid, reliable, and robust methodsfor assessing enantiomeric purity by conventional diode array detector assisted by chemometric tools.","palabras_clave":"Química Analítica|Cromatografía|Quimiometría"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5278,"idioma_id":4,"titulo":"IL-6 improves the nitric oxide-induced cytotoxic CD8+ T cell dysfunction in human chagas disease","resumen":"Reactive oxygen and nitrogen species are important microbicidal agents and are also involved in lymphocyte unresponsiveness during experimental infections. Many of the biological effects attributed to nitric oxide are mediated by peroxynitrites, which induce the nitration of immune cells, among others. Our group has demonstrated that nitric oxide is involved in the suppressive activity of myeloid-derived suppressor cells in Trypanosoma cruzi-infected mice, with a higher number of CD8+ T cells suffering surface-nitration compared to uninfected controls. Studying the functional and phenotypic features of peripheral CD8+ T cells from chagasic patients and human cells experimentally infected with T. cruzi, we found that different regulatory mechanisms impaired the effector functions of T cytotoxic population from seropositive patients. Peripheral leukocytes from chagasic patients showed increased nitric oxide production concomitant with increased tyrosine nitration of CD8+ T cells. Additionally, this cytotoxic population exhibited increased apoptotic rate, loss of the TCR&zeta;-chain, and lower levels of CD107a, a marker of degranulation. Strikingly, IL-6 stimulation of in vitro-infected peripheral blood mononuclear cells obtained from healthy donors, blunted T. cruzi-induced nitration of CD3+CD8+ cells, and increased their survival. Furthermore, the treatment of these cultures with an IL-6 neutralizing antibody increased the percentage of T. cruzi-induced CD8+ T cell nitration and raised the release of nitric oxide. The results suggest that the under-responsiveness of cytotoxic T cell population observed in the setting of long-term constant activation of the immune system could be reverted by the pleiotropic actions of IL-6, since this cytokine improves its survival and effector functions.","palabras_clave":"CD73|CD39|TRYPANOSOMA CRUZI INFECTION|OXIDATIVE STRESS|PEROXINITRITE|TYROSINE NITRATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5279,"idioma_id":4,"titulo":"Effect of the preparation method on the structure of linseed oil-filled poly(urea-formaldehyde) microcapsules","resumen":"Linseed oil was satisfactorily encapsulated in urea-formaldehyde (UF) shell by one acid stage polymerization.This was confirmed by thermal analyses (TGA) and FTIR studies. Microcapsules produced by one acid stage were strong enough to bear the preparation circumstances, and were dried into a free flowing powder, which remained stable under storage for 6 months at ambient laboratory conditions. Undesirableformation of UF particles in suspension that do not contribute to the shell growth was inhibited by selecting a proper amount of poly vinyl alcohol (PVA) and sodium dodecyl benzene sulfonate (SDBS), and adjusting the pH of the process.The surface morphology of the microcapsules was particularly sensitive to the pH of the reaction medium. At low pH values (&#8764;1.7) the UF nanoparticles deposited onto the microcapsule surface, thereby producing a strong shell. Conversely, microcapsules prepared at pH 3.5, did not maintain their mechanical integrity after drying because of the weak wall shell. Spherical microcapsules with diameters in the range of 50?200 mm were obtained under mechanical agitation at 600 rpm. The size was reduced to 1?20 mm using an ultrasonic homogenizer. Linseed oil could not be encapsulated by UF resin using the two-stage method. Under basic pH, UF nanoparticles remained in suspension and, consequently, the linseed oil was not completely microencapsulated. Results obtained in this research highlight the role of the pH of the reaction medium on the microencapsulation process of linseed oil by UF resins.","palabras_clave":"linseed oil|microcapsules|urea-formaldehyde"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5280,"idioma_id":4,"titulo":"SPARC gene deletion protects against toxic liver injury and is associated to an enhanced proliferative capacity and reduced oxidative stress response","resumen":"SPARC, also known as osteonectin and BM-40, is a matricellular protein witha number of biological functions. Hepatic SPARC expression is induced in responseto thioacetamide, bile-duct ligation, and acute injuries such as concanavalin A andlipopolysacharide (LPS)\/D-galactosamine. We have previously demonstrated that thetherapeutic inhibition of SPARC or SPARC gene deletion protected mice against liverinjury. We investigated the mechanisms involved in the protective effect of SPARCinhibition in mice. We performed a proteome analysis of livers from SPARC andSPARC mice chronically treated with thioacetamide. Catalase activity, carbonylationlevels, oxidative stress response, and mitochondrial function were studied. Genomicanalysis revealed that SPARC&#8722;\/&#8722; mice had an increased expression of cell proliferationgenes. Proteins involved in detoxification of reactive oxygen species such as catalase,peroxirredoxine-1, and glutathione-S-transferase P1 and Mu1 were highly expressedas evidenced by proteome analysis; hepatic catalase activity was increased inSPARC&#8722;\/&#8722;&#8722;\/&#8722; mice. Oxidative stress response and carbonylation levels were lower in liversfrom SPARC mice. Hepatic mitochondria showed lower levels of nitrogen reactivespecies in the SPARC&#8722;\/&#8722; concanavalin A-treated mice. Mitochondrial morphology waspreserved, and its complex activity reduced in SPARC&#8722;\/&#8722; mice. In conclusion, our datasuggest that the protection associated with SPARC gene deletion may be partially due &#8722;\/&#8722;to a higher proliferative capacity of hepatocytes and an enhanced oxidative stress defense in SPARCINTRODUCTION &#8722;\/&#8722; mice after liver injur","palabras_clave":"sparc|hepatocyte rpoliferation|reactive oxygen species"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5282,"idioma_id":5,"titulo":"Especies de Ulva del golfo San Jorge, Patagonia Argentina: variaciones bioquímicas estacionales - espaciales y su relación con la producción de metabolitos bioactivos","resumen":"Objetivos. Investigar especies de Ulva del Golfo San Jorge, Patagonia Argentina, integrando aspectosbioquímicos, actividad biológica y variaciones estacionales\/habitacionales que permitan determinar supotencial aplicación en Salud.Métodos. Las algas se colectaron estacionalmente en playas con y sin contaminación antrópica; se secaron,molieron y extrajeron con etanol. Los extractos se fraccionaron mediante solventes de distintapolaridad. Cada fracción fue caracterizada mediante reacciones químicas cualitativas y cromatografía.Las fracciones ricas en flavonoides se analizaron por HPLC-DAD y los lípidos, por TLC. La actividadbiológica se ensayó mediante el test de citotoxicidad de Artemia salina, inhibición de la elongación radicular,inhibición de la xantino oxidasa, actividad antioxidante y antibacteriana.Resultados. Se identificaron Ulva rigida y Ulva lactuca. Ambas evidenciaron flavonoides, taninos, quinonas,lípidos, carbohidratos, esteroides. Los flavonoides resultaron mayoritarios en U. rigida (otoño einvierno) y en U. lactuca (primavera e invierno), destacándose kayaflavona. Se detectaron alcaloides enUlva rigida (verano). Los lípidos fueron mayoritarios en verano; U. lactuca en invierno mostró monogalactosildiacilglicéridoy en otoño, digalactosilacilglicérido y fosfatidilcolina. La actividad inhibitoria dela elongación radicular, que presume actividad antitumoral, fue importante y dosis dependiente, destacándoseU. rigida de otoño y primavera (DE50= 480 y 520 µg\/ml, respectivamente). Las demás bioactividadesresultaron escasas o negativas.Conclusiones. Las variaciones metabólicas observadas muestran la importancia de considerar las condicionesespacio - temporales al planificar una colecta de ejemplares para su estudio y\/o aplicación. Esteprimer análisis integral de especies de Ulva de la Patagonia Argentina, evidencia su potencialidad comoantitumoral.","palabras_clave":"ULVA|FENOLES |ANTITUMORAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5283,"idioma_id":4,"titulo":"Two Aquaporins, multiple ways of assembly","resumen":"Protein oligomerization is a biological relevant event that may provide functional advantages to biological systems1. The association of aquaporin (AQP) protomers to form hetero-oligomeric assemblies is a current challenging area of research. PIP1 and PIP2, members of the plant AQP subfamily named PIP (for plant plasma membrane intrinsic proteins), have been intensively studied in the recent years particularly due to their ability to hetero-oligomerize.All AQPs share a common tetrameric structure, where each protomer has six membrane-spanning alpha helices (from 1 to 6) connected by five loops (from A to D), and loops B and E fold as half helices inserted in the membrane to form an individual pore; in this way each AQP protomer in the tetramer has a single active pore2. Members of PIP1 and PIP2 subgroups not only have the conserved tridimensional structure of the AQP family but also display a high degree of amino acid similarity between them. However, while most PIP1 protein is retained in the endoplasmic reticulum unless co-expressed with PIP2, PIP2 is effectively transported to the plasma membrane3-6. The routing modification of PIP1 from the endoplasmatic reticulum to the plasma membrane after its co-expression with PIP2 is due to PIP1-PIP2 physical interaction3,5 and although this interaction is well supported by experimental evidences, the biophysical aspects and the molecular mechanisms of this event have been identified only recently.7,8We investigated the full characterization of the biological and biophysical properties of the different hetero-oligomeric configurations formed by red beet PIP1 and PIP2 subunits. Our results showed that PIP1 and PIP2 protomers have the ability to assemble with multiple stoichiometries, giving rise to 3:1, 1:3 or 2:2 heterotetramers, and all these tetramers are localized at the plasma membrane7. A variable stoichiometry has also been proposed for strawberry PIPs4 and, recently, a similar result was confirmed for maize PIPs8. In the case of red beet PIPs, we showed that although all stoichiometric assemblies can be formed, which one prevails depends on PIP1 and PIP2 relative expression. If one PIP monomer (PIP1 or PIP2) outnumbers the other, the assembly of 1:3 (or 3:1) heterotetramers is favored and even homotetramers of PIP1 or PIP2 can be the predominant species in the cell.It must be stressed that even though the association of PIP protomers occurs with random stoichiometry, their precise interactions are controlled by specific protomer contacts. The absence of these contacts can affect their biological activity8 and even preclude their packing within heterotetramers6. In this regard, there are many lines of evidence that minor but critical changes in loop A, loop E and transmembrane domains, i.e. single amino acid mutations, are sufficient to produce changes in protomer interaction and, as a consequence of structural rearrangements, modifications in the functional proerties of the PIP tetramers occur.Interestingly, in the case of red beet PIPs, we found that the water permeability behavior of all PIP1-PIP2 heterotetrameric species is the same. Nevertheless, PIP1-PIP2 heterotetramers display major differences compared to homotetramer activity: i- the water transport of heterotetramers is twice the contribution of PIP2 homotetramers, while the contribution of the PIP1 homotetramer is null as they are not able to reach the plasma membrane, ii- all PIP1-PIP2 heterotetrameric species show the same Pf (osmotic water permeability coefficient) inhibition by intracellular pH, but a clear differential regulation in comparison with PIP2 homotetramers, as the closure of PIP2 homotetramers occurs at more acidic values. The only characteristic shared between PIP1-PIP2 heterotetramers and PIP2 homotetramers is the degree of cooperativity for proton sensing: all red beet PIP tetramers display not only a positive cooperativity response, but also a sigmoidal behavior for pH induced Pf block with a similar extent of cooperativity (Figure 1).Figure 1. Biological properties of all possible PIP stoichiometric tetramers. Arrows represent the expression level of PIP1\/PIP2; &#952;, PIP tetramer stoichiometry; &#937; (10&#8722;4 cm s&#8722;1 ng&#8722;1), Pf contribution of each tetrameric species at the plasma membrane; pH0.5, pH at which the Pf change is half maximal; n, Hill coefficient; and &#945;, cellular localization of tetramers. PM corresponds to plasma membrane and IN indicates intracellular localization. Disclosure of potential conflicts of interest: No potential conflicts of interest were disclosed.The results obtained for PIP1 and PIP2 interactions show that the mixture in plasma membrane of different amounts of PIP2 homotetramers and\/or PIP1-PIP2 heterotetramers allow variable water permeability and different apparent cooperativity for the opening and closing of these channels. Remarkably, only two PIP aquaporins can give rise to a wide spectrum of water transport modes. In this way, the membrane water permeability can be modulated by both the relative amounts of each kind of PIP tetramer confined in the membrane and the intracellular proton concentration. These findings represent a novel regulatory mechanism to adjust water transport across the plant plasma membrane. Moreover it confirms that the biophysical studies of intersubunit protein interactions may shed light on how quaternary structures endow the protomers with specific biological properties in oligomeric channels.","palabras_clave":"OLIGOMERIZATION|AQUAPORINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5285,"idioma_id":4,"titulo":"Detection of plant growth enhancing features in psychrotolerant yeasts from Patagonia (Argentina)","resumen":"This study explores the biotechnological potential for plant production of twelve psychrotolerant yeasts strains from Northwest-Patagonia. These strains were isolated from different substrates associated with Nothofagus sp. in native forests and Vaccinium sp. in a commercial plantation. Yeasts characterization was performed using in-vitro assays to evaluate the production of auxin-like compounds and siderophores, ability to solubilize inorganic phosphate and to reduce common plant pathogen growth. Strain YF8.3 identified as Aureobasidium pullullans was the main producer of auxin-like and siderophores compounds. Phosphate solubilization was a characteristic observed by strains L8.12 and CRUB1775 identified as Holtermaniella takashimae and Candida maritima, respectively. Different yeast strains were able to inhibit the growth of Verticillium dahliae PPRI5569 and Pythium aphanidermatum PPRI 9009, but they all failed to inhibit the growth of Fusarium oxysporum PPRI5457. The present study suggests that yeasts present in different environments in Northwestern-Patagonian have physiological in-vitro features which may influence plant growth. These results are promising for the developing of biological products based on Patagonian yeasts for plant production in cold-temperate regions.","palabras_clave":"auxin|siderophores|phosphate solubilization|phytopathogens inhibition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5286,"idioma_id":4,"titulo":"Dilution effects in spin 7\/2 systems. The case of the antiferromagnet GdRhIn5","resumen":"We report the structural and magnetic characterization of La-substituted Gd 1 &#8722; x La x RhIn 5 ( x &#8804; 0.50 ) anti-ferromagnetic (AFM) compounds. The magnetic responses of pure GdRhIn 5 are well described by a S=7\/2 Heisenberg model. When Gd 3+ ions are substituted by La 3+ , the maximum of the susceptibility and the inflection point of the magnetic specific heat are systematically shifted to lower temperatures accompanied by a broadening of the transition. The data is qualitatively explained by a phenomenological model which incorporates a distribution of magnetic regions with different transition temperatures (T N ). The universal behaviour of the low temperature specific heat is found for La (vacancies) concentrations below x=0.40 which is consistent with spin wave excitations. For x=0.5 this universal behaviour is lost.The sharp second order transition of GdRhIn 5 is destroyed, as seen in the specific heat data, contrary to what is expected for a Heisenberg model. The results are discussed in the context of the magnetic behaviour observed for the La-substituted (Ce,Tb,Nd)RhIn 5 compounds.","palabras_clave":"Magnetism|Dilution effects|Heisenberg model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5287,"idioma_id":4,"titulo":"Transcriptome response mediated by cold stress in Lotus japonicus","resumen":"Members of the Lotus genus are important as agricultural forage sources under marginal environmental conditions given their high nutritional value and tolerance of various abiotic stresses. However, their dry matter production is drastically reduced in cooler seasons, while their response to such conditions is not well studied. This paper analyzes cold acclimation of the genus by studying Lotus japonicus over a stress period of 24 h. High-throughput RNA sequencing was used to identify and classify 1077 differentially expressed genes, of which 713 were up-regulated and 364 were down-regulated. Up-regulated genes were principally related to lipid, cell wall, phenylpropanoid, sugar, and proline regulation, while down-regulated genes affected the photosynthetic process and chloroplast development. Together, a total of 41 cold-inducible transcription factors were identified, including members of the AP2\/ERF, NAC, MYB, and WRKY families; two of them were described as putative novel transcription factors. Finally, DREB1\/CBFs were described with respect to their cold stress expression profiles. This is the first transcriptome profiling of the model legume L. japonicus under cold stress. Data obtained may be useful in identifying candidate genes for breeding modified species of forage legumes that more readily acclimate to low temperatures","palabras_clave":"LEGUME|LOTUS SPP|COLD STRESS|TRANSCRIPTOME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5288,"idioma_id":4,"titulo":"Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature","resumen":"Lotus species are important forage legumes due to their high nutritional value and adaptability to marginal conditions. However, the dry matter production and regrowth rate of cultivable Lotus spp. is drastically reduced during colder seasons. In this work, we evaluated the chilling response of L. japonicus ecotypes MG-1 and MG-20. No significant increases were observed in reactive oxygen species and nitric oxide production or in lipid peroxidation, although a chilling-induced redox imbalance was suggested through NADPH\/NADP+ ratio alterations. Antioxidant enzyme catalase, ascorbate peroxidase, and superoxide dismutase activities were also measured. Superoxide dismutase, in particular the chloroplastic isoform, showed different activity for different ecotypes and treatments. Stress-induced photoinhibition also differentially influenced both ecotypes, with MG-1 more affected than MG-20. Data showed that the D2 PSII subunit was more affected than D1 after 1 d of low temperature exposure, although its protein levels recovered over the course of the experiment. Interestingly, D2 recovery was accompanied by improvements in photosynthetic parameters (Asat and Fv\/Fm) and the NADPH\/NADP+ ratio. Our results suggest that the D2 protein is involved in the acclimation response of L. japonicus to low temperature. This may provide a deeper insight into the chilling tolerance mechanisms of the Lotus genus.","palabras_clave":"CHILLING|D2 PROTEIN|PHOTOINHIBITION|ROS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5289,"idioma_id":4,"titulo":"Soil water regime of grassland communities along subtle topographic gradient in the Flooding Pampa (Argentina)","resumen":"Three plant communities positioned along a subtle topographic gradient, referred to as upland,intermediate and lowland positions, characterize the landscape of the Flooding Pampa Grasslands of Argentina. Although it is believed that the structure and functioning of plant communities at each position are in close relationship with their hydric regime, this has never been quantified. More than 800 measurements of soil water content during four years, along with soil water retention curves, and physical and chemical parameters of soils were assessed at each position. Results showed that water availability along the year varied among positions in accordance to differences in hydrological balance and soil water retention capacity of each of them. Water retention increased in relation to clay and organic matter content from the upland to the lowland position. The upland position, with more soil sand content, registered severe drought events during late spring and summer, without flooding periods in any season. The intermediate and lowland positions, with more soil clay content, remained flooded for several weeks during winter and spring, and they manifested less severe summer droughtsthan upland position. Moreover, the lowland position resulted more hydromorphic than the intermediate one. These spatial and temporal variations of water regime and soil parameters characterizing the upland, intermediate and lowland positions concur with different plant communities associated to each of them.","palabras_clave":"DROUGHT|FLOODING|SOIL WATER RETENTION CURVES|PAMPA GRASSLANDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5290,"idioma_id":5,"titulo":"¿A quiénes le enseñamos y qué contenidos elegimos?.. Repensando la contextualización y la producción de conocimiento en el aula desde -y para- la enseñanza de la antropología","resumen":"Este artículo presenta reflexiones sobre una dimensión que consideramos central en el desarrollo delquehacer docente y, a la vez, se liga a nuestra formación disciplinar: la importancia de la contextualizaciónpara la enseñanza de la antropología. Para ello, recuperamos algunas cuestiones registradas entorno al tema desde nuestras experiencias docentes en una materia que, dentro de la carrera de Antropología,se encarga curricularmente de la formación de profesores. Desde la perspectiva del enfoquesocioantropológico argumentamos la importancia de incorporar el trabajo etnográfico como parte delproceso que conlleva la elaboración de una propuesta pedagógica y donde la contextualización aparececomo un ejercicio complejo. En este sentido, afirmamos que la producción de cualquier planificacióndidáctica debe contemplar un acercamiento etnográfico al contexto formativo para documentar lasrelaciones sociales que se despliegan entre los distintos sujetos y los saberes, con el propósito de conoceren su complejidad los procesos de producción de conocimiento en el aula. Asimismo, analizamosuna experiencia posible de programación desarrollada en el marco de nuestra materia, para dar cuentade las dimensiones involucradas en el ejercicio de contextualizar, sin dejar de lado las dificultadesencontradas en la construcción de prácticas de la enseñanza, muchas veces ligadas a una tendenciapor asociar contextualización a ?diagnóstico? proveniente del campo de la pedagogía. Por tanto, aquípretendemos brindar aportes sobre formas de enseñar específicamente situadas que, en sintonía con elquehacer etnográfico, recuperan las voces y prácticas de los sujetos buscando antropologizar la didácticay trascender modelos prescriptivos que tipifican.","palabras_clave":"CONTEXTUALIZACIÓN|PRÁCTICAS DE LA ENSEÑANZA|PRODUCCIÓN DE CONOCIMIENTO|ENFOQUE SOCIOANTROPOLOGICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5291,"idioma_id":4,"titulo":"Phase transformation and shape memory effect of a Cu-Al-Ni-Mn-Nb high temperature shape memory alloy","resumen":"The microstructure, phase transformation and shape memory effect of the high temperature shape memory alloy Cu-11.4Al-3.2Ni-3Mn-0.5 Nb (wt%) were investigated for the first time. The shape recovery ratio can achieve 73% when submitted to 690 MPa and the compressive stress of fracture of the alloy is 1560 MPa.","palabras_clave":"Cooper Alloys|Shape Memory Alloys|Phase Transformation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5292,"idioma_id":4,"titulo":"Effect of Al addition to Rapidly Solidified Mg-Cu-Rare Earth Alloys","resumen":"Rapidly solidified Mg based alloys are of interest for industrial applications as a structural material and for hydrogen storage. Mg-Cu-Rare Earth alloys have shown high glass forming ability; full amorphous structure with thickness of mm size can be obtained within these systems. However, their brittle behavior limits their industrial applications. In the present work, the Al effect in substitution of Cu in the Mg65Cu25MM10 (at%, MM: mischmetal) was studied. Samples up to 15at% Al were prepared by splat cooling and their microstructure, stability and mechanical properties were characterised.The crystallization temperature increases with the Al addition; the amorphous phase with different Al content has a Young?s modulus of ~55GPa; the microhardness increases with the Al content in the amorphous and crystallized samples and the fracture of the alloy containing 10at% Al showed ductile vein patterns characteristics of ductile metallic glasses. The partial Cu substitution by Al can improve the stability and mechanical properties of the amorphous Mg65Cu25MM10 alloy.","palabras_clave":"Magnesium|Rapid Solidification|Amorphous"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5293,"idioma_id":4,"titulo":"Effect of alloying elements in melt spun Mg-alloys for hydrogen storage","resumen":"In this paper we report the effect of alloying elements on hydrogen storage properties of melt spun Mg-based alloys. The base alloys Mg90Si10, Mg90Cu10, Mg65Cu35 (at%) were studied. We also investigated the effect of rare earths (using MM: mischmetal) and Al in Mg65Cu25Al10, Mg65Cu25MM10 and Mg65Cu10Al15MM10 alloys. All the melt-spun alloys without MM show a crystalline structure, and the Mg65Cu25MM10 and Mg65Cu10Al15MM10 alloys showed an amorphous and partially amorphous structure respectively. At 350&#730;C all the alloys had a crystalline structure during the hydrogen absorption-desorption tests. It was observed that Si and Cu in the binaries alloys hindered completely the activation of the hydrogen absorption.The partial substitution of Cu by MM or Al allowed activation. The combined substitution of Cu by MM and Al showed the best results with the fastest absorption and desorption kinetics, which suggests that this combination can be used for new Mg-alloys to improve hydrogen storage properties.","palabras_clave":"Hydrogen Storage|Magnesium|Rapid Solidification|Nanomaterials"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5295,"idioma_id":4,"titulo":"Environmental variables associated with hantavirus reservoirs and other small rodent species in two national parks in the Paraná delta, Argentina. Implications for disease prevention","resumen":"Hantavirus pulmonary syndrome (HPS) is a severe zoonotic disease caused by hantaviruses hosted in variousrodentsspecies. In Argentina, its transmission to humans has been associated to exposure during activities such as farming, recreation, and tourism which are carried out in wild and rural areas. The aim of this study was to analyze the macro- and micro-habitat use and spatio?temporal variation of small sylvan rodents in Pre Delta and Islas de Santa Fe national parks,located in an HPS-endemic area of Argentina. Rodent communities were studied at six sites: two islands, a riparian forest, an inland forest, a marsh, and the margins of a pond. A total of 453 individuals of &#64257;ve species were captured with a trapping effort of 9471trap-nights. Maximum species richness was found at the marsh and the pond margin sites. Abundance of rodents was in&#64258;uenced by &#64258;ooding events. Two hantavirus reservoirs, Oligoryzomys &#64258;avescens and Akodon azarae, were identi&#64257;ed in the area. O. &#64258;avescens was captured in every habitat, but it was dominant in Islas de Santa Fe National Park where its abundance was strongly in&#64258;uenced by &#64258;ooding. A. azarae was captured in every habitat except on the islands. A. azarae behaved as a generalist species at a micro-habitat scale in every habitat of PreDelta National Park except for the marsh where it selected patches with low vegetation height. Based on these results, several disease prevention measures, including the use of rodent-proof containers for food,and keeping the grass short in the campsite, are proposed in order to reduce the risk to visitors and residents of contracting HPS.","palabras_clave":"HANTAVIRUS|ENVIRONMENTAL FACTORS|HABITAT USE|NATIONAL PARKS|RESERVOIRS|RODENTS|ECOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5296,"idioma_id":4,"titulo":"CHRONIC ADULT T-CELL LEUKEMIA IN A YOUNG MALE AFTER BLOOD TRANSFUSION AS A NEWBORN","resumen":"Human T-cell Lymphotropic virus type 1 (HTLV-1) is the etiological agent of Adult T-cell Leukemia\/Lymphoma (ATLL) and HTLV-1 Associated Myelopathy\/Tropical Spastic Paraparesis (HTM\/TSP). Areas of extremely high HTLV-1 prevalence are surrounded by areas of middle or very low prevalence. ATLL is an aggressive lymphoproliferative malignancy of peripheral T cells, with an incidence of less than 5% in HTLV-1-infected individuals. ATLL develops in the majority of cases in individuals who were infected with HTLV-1 by their mothers due to prolonged breastfeeding. In non-endemic areas, ATLL is usually limited to immigrants from endemic regions. Very few cases of ATLL have been diagnosed in recipient patients few years after an organ transplantation or blood transfusion worldwide. Achieving an accurate and fast diagnosis of ATLL can be challenging due to the lack of professional experience, delayed consultation and difficulty in its subclassification. We present a case of a delayed onset of a chronic ATLL in an 18-years-old male that was transfused with blood components as a premature newborn in Buenos Aires, a non-endemic city of South America.","palabras_clave":"ATLL|transfusion|newborn|chronic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5297,"idioma_id":4,"titulo":"DEVELOPMENT AND FIELD EVALUATION OF LIQUID INOCULANTS WITH NATIVE Bradyrhizobial STRAINS FOR PEANUT PRODUCTION","resumen":"A critical process in the leguminous crops cycles is biological nitrogen fixation (BNF). Application of inoculantswith N fixing bacteria is economically and environmentally favourable. The aim of this work was to selectcompetitive native peanut microsymbionts, evaluate their survival in inoculant support and assess their impacton peanut (Arachis hypogaea L.) production under field conditions at Córdoba province in Argentina. Theefficient N fixing Bradyrhizobium sp. J-81 and Bradyrhizobium sp. J-237, previously obtained from peanutnodules in the region of Cordoba, Argentina, were evaluated. In microcosm assays, plants inoculated with theseisolates demonstrated better symbiotic parameters than those inoculated with reference strains. Different bacterialgrowth media and inoculant stabiliser solutions were evaluated. Balanced medium and arabic gum stabilisingsolution had optimal bacterial growth and the highest bacterial concentration and viability, respectively. Inoculationwith either inoculants resulted in 44% greater peanut pod yield at Pizarro compared to the non-inoculated plants,although no significant differences were found with respect to commercial inoculants treatments.","palabras_clave":"INOCULANTS|PEANUT|BRADYRHIZOBIUM|FIELD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5298,"idioma_id":4,"titulo":"Role of  bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp.  S119","resumen":"The aim of this study was to analyze if cofactor pyrroquinoline quinone from Serratia sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain Serratia sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant Serratia sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within Serratia strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from Serratia sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of Serratia sp. S119 but is not required for growth promotion of peanut and maize plants.","palabras_clave":"PQQ cofactor|Serratia sp|PHOSPHATE SOLUBILIZATION|PLANT GROWTH PROMOTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5299,"idioma_id":4,"titulo":"Removal of Uranium (VI) with Iron Nanoparticles","resumen":"In this work, the removal efficiency of U(VI) from water using commercial nanoparticles of zerovalent iron(nZVI) (NANOFER 25, NANO IRON s.r.o.) and magnetite (nM) (NanoFe®, Nanotek SA) was evaluated. Batchexperiments were carried out in a jacketed reactor with a vertical paddle stirrer, using UO2(NO3)2 solutions([U(VI)]0 = 0.25 mM &#8801; 59.5 mg L-1) at pH 5.3. The nanoparticles (initially suspended in water) were added to the U(VI) solution to achieve different Fe:U(VI) molar ratios (MR) in the range of 1 to 100. U(VI) removal with nZVI and nM at MR 4 and dissolved oxygen (DO) levels higher than 0.1 mg L-1 were rather efficient, reaching in both cases a final removal of 65 %. Under these conditions, uranium removal strongly depends on the presence of DO, decreasing with increasing DO. When 40 and 100 MR were used, a complete U(VI) removal in the first 15 min of treatment was observed, and oxygen was consumed reaching negligible DO levels (below 0.1 mg L-1). With MR = 4 and DO levels below 0.1 mg L-1 (achieved by N2 bubbling), removal of U(VI) was complete in 60 min of reaction for both types of nanoparticles. Although the trend of the removal curves was similar, the advantage of nM is that very low levels of iron in solution (as Fe(total)), below 1 mg L-1, were observed during the whole reaction time, while it was 5 mg L-1 for nZVI at the end of the run, mainly as Fe(II). Analysis of the final solids by XANES and Raman spectroscopies revealed the presence of uranium, probably as UO2.","palabras_clave":"URANIUM REMOVAL|IRON NANOPARTICLES|CATALYSIS|XANES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5301,"idioma_id":4,"titulo":"Genotype and Nitrogen Effects over Maize Kernel Hardness and Endosperm Zein Profiles","resumen":"Maize (Zea mays L.) kernel hardness is of utmost importance for dry-milling processors. Zeins, maize prolamins, are known to be key proteins affecting this trait. We investigated the response of kernel zein profiles to N fertilization in maize hybrids with contrasting kernel hardness (measured as test weight, vitreousness, kernel density and floaters percentage). A field experiment was done during two seasons, with three N fertilization treatments and four commercial hybrids with different hardness (two flint and two dent kernel types). We also measured yield, kernel protein concentration, and zein profiles (Z2, or &#947; and &#946;, and Z1, or &#945; and &#948;). Flint kernel type always yielded less and showed higher kernel hardness indicators when compared to dents (P<0.01). N fertilization helped increase yield and kernel hardness in both kernel types. Flint kernel type had consistently more Z2 than dents, while only in one year Z1 was higher in flints. Dent kernel type had a Z1\/Z2 ratio similar to or higher than flints. Increasing N resulted in increased concentration of both zein types, but the effect was more pronounced on Z1. Significant correlations were observed between the different zein types (Z1 and Z2) and hardness indicators and total protein concentration, but Z2 showed the highest correlations with all kernel hardness traits. Our results expand previous knowledge on genotype and N fertilization effects over zein profiles and their involvement in kernel physical characteristics relevant for dry milling.","palabras_clave":"Zea mays L.|kernel quality|zein profile|kernel hardness|flint|dent"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5302,"idioma_id":5,"titulo":"Prácticas deformatorias en el sitio Tres Cruces I (Salta, Argentina) durante el período Formativo Superior: caracterización y análisis","resumen":"Este trabajo ofrece información inédita referida a los tipos deformatorios documentados a la fecha para la Quebrada del Toro (Salta, Argentina) durante el período Formativo Superior. La muestra analizada proviene de la excavación de la Estructura 3 del sitio Tres Cruces I y se trata de los únicos contextos bioantropológicos recuperados y estudiados sistemáticamente para el área con esta cronología (400 - 900 dC.). Se observa una alta presencia de la práctica y la asociación de ciertos tipos con el sexo de los individuos. Por otra parte, no se registra un vínculo entre el tipo deformatorio y el tratamiento mortuorio diferencial de los individuos. Se establecen semejanzas y variaciones en la presencia y frecuencia de los tipos deformatoriosdocumentados respecto de los observados en muestras procedentes de sectores geográficos con los cuales se registran evidencias de interacción. Además, de su comparación con otros contextos documentados para el área, con fechados más tempranos o posteriores, surge que existen cambios en la manera en que los tipos se distribuyen en el tiempo.","palabras_clave":"DEFORMACION CRANEANA|FORMATIVO|QUEBRADA DEL TORO|VARIACIONES TEMPORALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5303,"idioma_id":5,"titulo":"Análisis de preservación ósea y dentaria en dos grupos etarios: su importancia en el estudio de conjuntos esqueléticos","resumen":"Se presentan aquí los resultados del análisis de preservación de restos óseos y dentarios humanos de individuos adultos y subadultos provenientes de un cementerio contemporáneo. La representatividad, completitud y preservación de los elementos óseos y dentarios son fundamentales en estudios bioantropológicos, dado que dichos atributos posibilitan la definición de estrategias de investigación que conduzcan a interpretaciones demográficas y sociales. La condición y composición de los conjuntos osteológicos debe ser analizada teniendo en cuenta la relación entre factores intrínsecos y extrínsecos o tafonómicos que afectan su preservación. Para el análisis de la preservación, se tuvieron en cuenta tres dimensiones: la representación ósea y dentaria, el grado de fragmentación y el grado de deterioro y completitud del hueso. Para ello, se utilizaron el Índice de Representación Ósea (IRO), el Índice de Representación Dentaria (IRD), el Índice de Fragmentación Ósea (IFO), el Índice de Preservación Anatómica (IPA) y el Índice de Preservación del Hueso Cortical (IPC). Los resultados obtenidos mostraron diferencias significativas en la preservación entre individuos adultos y subadultos, presentando el IRO, IFO, IPA e IPC valores mayores en adultos lo que se explicaría por diferencias en las características de los tejidos esqueléticos en diferentes momentos del desarrollo.","palabras_clave":"COLECCION OSTEOLOGICA|PRESERVACION|ADULTO|SUBADULTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5304,"idioma_id":5,"titulo":"Caracterizacion de la lana de ovejas criollas argentinas en cuatro ambientes diferentes","resumen":"La raza ovina Criolla es la fundadora del patrimonio ovino argentino y está distribuida en casi todas las provincias. Son pocos los trabajos destinados a estudiar la calidad lanera de este germoplasma. El objetivo fue caracterizar la lana de ovejas Criollas Argentinas, utilizando las principales medidas que determinan su calidad y su valor comercial. El muestreo se realizó sobre majadas ubicadas en cuatro provincias: Salta (SA) (n= 44); Santiago del Estero (SE) (n= 60); Corrientes (CO) (n= 40) y Buenos Aires (BA) (n= 59). Se tomaron muestrasde lana de cada oveja y se determinaron las siguientes variables: diámetro medio de fibra (DMF); desvío estándar del diámetro medio de fibra (DE_DMF); curvaturade ondulación (CU); factor de confort (FC) y largo de mecha (LM). El análisis estadístico incluyó análisis de varianzas (ANOVA), análisis de componentes principales (ACP) y de conglomerados (ACO). Las ovejas SA se destacaron del resto de las regiones por su menor DMF= 26,1 ± 2,5µm y DE_DMF=6,2 ± 1,5µm, su mayor CU= 78,5 ± 13,5º\/mm y su mayor FC= 78,8 ± 12,7%, mientras que las de BA presentaron un LM= 123,0 ± 36,6mm, ampliamente superior a las otras regiones y CO presentó mayor DMF= 35,9 ± 4,1µm. El ACP identificó 2 componentes que Explicaron el 90% de la variabilidad. La primera diferencia SA de BA y muestrasuperposición entre SE y CO. La segunda diferencia BA del resto de las regiones. Del ACO surge que los ovinos de SE y CO forman un solo grupo definiéndose tres regiones: SA, BA y SE-CO. Ninguno de los tres grupos respondió a la categoría carpet-wool o Criola correspondiente a la clasificación lanera oficial, por eso se considera necesario construir un mapa lanero Criollo de la República Argentina.","palabras_clave":"Ovinos Criollos. | Fibras. | Calidad. | Característica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5305,"idioma_id":4,"titulo":"Performance of recycled concretes exposed to sulphate soil for 10 years","resumen":"Recycled aggregate concretes, particularly those made with crushed concrete in replacement of the natural coarse aggregate, have been increasingly used in the last decades. Although compressive strength is not affected by the replacement of conventional coarse aggregates by recycled coarse aggregates (RCA), there is still some disagreement regarding their durable performance, particularly when they are exposed to severe conditions.This study evaluates the performance of recycled concretes made with different RCA contents (25%, 50%, 75% and 100%) exposed to sulphate soil for more than 10 years. Concretes designed with and without provisions for durability to sulphate attack were exposed. The specimens were half buried in sulphate soil and their condition was periodically assessed by visual inspection, weight loss and dynamic modulus of elasticity. Additionally, the extent of the attack on mortar?coarse aggregates interfaces was studied by stereomicroscopy and optical microscopy. Results to date indicate similar performances for recycled aggregate concretes and natural aggregate concretes with the same compressive strength level, even for a 100% RCA content.","palabras_clave":"RECYCLED CONCRETE|DURABILITY|SULPHATE SOIL|SULPHATE ATTACK|SERVICE CONDITIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5306,"idioma_id":4,"titulo":"Dietary (-)-epicatechin mitigates oxidative stress, NO metabolism alterations, and inflammation in renal cortex from fructose-fed rats","resumen":"High fructose consumption has been associated to deleterious metabolic conditions. In the kidney, high fructose causes renal alterations that contribute to the development of chronic kidney disease. Evidence suggests that dietary flavonoids have the ability to prevent\/attenuate risk factors of chronic diseases. This work investigated the capacity of (-)-epicatechin to prevent the renal damage induced by high fructose consumption in rats. Male Sprague Dawley rats received 10% (w\/v) fructose in the drinking water for 8 weeks, with or without supplementation with (-)-epicatechin (20 mg\/kg body weight\/d) in the rat chow diet. Results showed that, in the presence of mild proteinuria, the renal cortex from fructose-fed rats exhibited fibrosis and decreases in nephrin, synaptopodin, and WT1, all indicators of podocyte function in association with: (i) increased markers of oxidative stress; (ii) modifications in the determinants of NO bioavailability, i.e., NO synthase (NOS) activity and expression; and (iii) development of a pro-inflammatory condition, manifested as NF-&#954;B activation, and associated with high expression of TNF&#945;, iNOS, and IL-6. Dietary supplementation with (-)-epicatechin prevented or ameliorated the adverse effects of high fructose consumption. These results suggest that (-)-epicatechin ingestion would benefit when renal alterations occur associated with inflammation or metabolic diseases.","palabras_clave":"ANTIOXIDANTS|FLAVONOIDS|HYPERTENSION|POLYPHENOLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5307,"idioma_id":5,"titulo":"Calibración de datos observados de contenido de agua en el suelo con sensores capacitivos","resumen":"Soil moisture or soil water content is an important variable for Meteorology, Agronomy, Hydrology and Soil Science. In Argentina, measurements of soil moisture are both spatially and temporally sparsely distributed. There are different methodologies of measuring soil moisture content like the gravimetric, neutron and dielectric methods, among others. During the growing season 2012-2013, soil moisture measurements were performed in a soybean crop field. The aim of this paper is to calibrate observed capacitive data with gravimetric and neutron methods as well as to build a high temporal resolution soil moisture database. Linear functions were used to calibrate data for each depth of measurement with the exception of the two deepest levels where retrieved functions have a poor data fitting. The quality of the observed data and the calibration functions have direct impact on the capacitive sensor errors. Gravimetric, neutron and capacitive soil moisture measurements were interpolated with the Kriging method to construct the high resolution database. Variability of soil water content was observed at different soil depths due to precipitation and plant water uptake.","palabras_clave":"Argiudol Típico|Sonda de neutrones|Función lineal de calibración"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5308,"idioma_id":4,"titulo":"Bovine viral diarrhea virus infects monocyte-derived bovine dendritic cells by an E2-mediated mechanism and transiently impairs antigen presentation","resumen":"Infection of professional antigen presenting cells by viruses can have a marked effect on these cells and important consequences for the generation of the subsequent immune responses. Here we demonstrate that different strains of bovine viral diarrhea virus (BVDV) infect bovine dendritic cells differentiated from non-adherent peripheral monocytes (moDC). BVDV did not cause apoptosis in these cells. Infection of moDC was prevented by incubating the virus with anti-E2 antibodies or by pre-treating the cells with recombinant E2 protein prior to BVDV contact, suggesting that BVDV infects moDC through an E2-mediated mechanism. Virus entry was not reduced by incubating moDC with Mannan or EDTA prior to infection, suggesting that Ca(2+) and mannose receptor dependent pathways are not mediating BVDV entry to moDC. Infected moDC did not completely up-regulate maturation surface markers. Infection, but not treatment with inactivated virus, prevented moDC to present a third party antigen to primed CD4+ T cells within the first 24 hours post infection (hpi). Antigen presenting capacity was recovered when viral replication diminished at 48 hpi, suggesting that active infection may interfere with moDC maturation. Altogether, our results suggest an important role of infected dendritic cells in BVDV-induced immunopathogenesis.","palabras_clave":"BOVINE VIRAL DIARRHEA VIRUS|DENDRITIC CELLS|VIRUS ENTRY|ANTIGEN PRESENTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5309,"idioma_id":4,"titulo":"Electrochemical sensor for amino acids and glucose based on glassy carbon electrodes modified with multi-walled carbon nanotubes and copper microparticles dispersed in polyethylenimine","resumen":"This work reports the analytical performance of glassycarbon electrodes (GCE) modified with multi-walled carbon nanotubes (CNT) andcopper microparticles dispersed in polyethylenimine (PEI) (GCE\/CNT-PEI-Cu) forthe quantification of amino acids, albumin and glucose. The best analyticalperformance was obtained with CNT-PEI-Cu prepared by sonicating for 15.0 min amixture of 1.0 mgmL-1 PEI, 1.0 mgmL-1 CNT and 3.0 mgmL-1copper. In the case of amino acids and albumin, the analytical signal wasobtained from the increase of copper oxidation signal produced as a consequenceof the complex formation between Cu(II) and the amino acids. The sensor allowedthe highly sensitive (submicromolar levels) and reproducible (3.9 %) amperometricquantification of hystidine, serine and cysteine at very low potentials (0.000V) and pH 7.40. Albumin was quantified by Square Wave Voltammetry after 10.0min interaction at -0.100 V with detection limits of 1.2 mg mL-1.GCE\/CNT-PEI-Cu was also used for the quantification ofglucose by amperometry at 0.700 V in a 0.100 M sodium hydroxide solutionthrough the known catalytic activity of copper towards the oxidation of glucose,with highly competitive detection limits (182 nM). GCE\/CNT-PEI-Cu wassuccessfully used for the quantification of amino acids and albumin in pharmaceuticalproducts and carbohydrates in beverages.","palabras_clave":"CARBON NANOTUBES|POLYETHILENIMINE|COOPER|AMINO ACID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5310,"idioma_id":4,"titulo":"Gaining flexibility in the design of microstructure, texture and shape memory properties of an Fe-Mn-Si-Cr-Ni alloy processed by ECAE and annealing","resumen":"The effect of ECAE on the properties of an Fe-15Mn-5Si-9Cr-5Ni shape memory alloy (SMA) was investigated. Phases, texture, microstructure, critical temperatures, mechanical and SM properties after severe deformation, and the effect of annealing were analysed. One ECAE pass develops a shear texture. There is a large &#12296;101&#12297; component in the extrusion direction, which is near the ideal &#12296;414&#12297; for martensitic transformation activation. The pressing did not refine the previous as cast structure but introduced dislocations and &#949;-martensite. Thus, the stress to induce the &#947;&nbsp;&#8594;&nbsp;&#949; martensitic transformation, &#963;&#947;&nbsp;&#8594;&nbsp;&#949;, reached 620&nbsp;MPa, and the quantity of &#949;-martensite formed after compression was limited. The degree of shape recovery was reduced, while the reverse transformation temperatures increased. Heat treatments above 800&nbsp;°C activated recrystallization. As annealing temperature increased, new grains nucleated and grew, reaching a size of 53&nbsp;&#956;m after heating to 1000&nbsp;°C. For this condition, &#963;&#947;&nbsp;&#8594;&nbsp;&#949; was 430&nbsp;MPa, and the best shape recovery was achieved, 64%.","palabras_clave":"FERROUS SHAPE MEMORY ALLOYS|ECAE|MARTENSITIC TRANSFORMATION|MICROSTRUCTURE|X-RAY DIFFRACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5311,"idioma_id":4,"titulo":"A new Eocene dasypodid with caniniforms (Mammalia, Xenarthra, Cingulata) from Northwest Argentina","resumen":"Abstract The aim of this work is to present a new genus and species of Dasypodidae from the Lumbrera Formation(lower Lumbrera), early-middle Eocene of Salta Province,northwest Argentina. The new taxon, documented by one specimen, consists of an incomplete skull and jaw with teeth housed in their alveoli, postcranial remains, and isolated osteoderms. Lumbreratherium oblitum, gen. et sp. nov., is characterized by heterodont dentition, with a caniniform as the first tooth, diastema between caniniform and first molariform, teeth with closed roots, and a peculiar morphology of the osteoderms. A phylogenetic analysis indicates that Lumbreratherium oblitum, gen. et sp. nov., and Pucatheriumparvum belong to a monophyletic clade in a basal position within the Cingulata. The singularity of the morphological characteristics of these Paleogene armadillos of northwest Argentina reinforces the hypothesis of an intertropical origin of mammal clades different from those of the Paleogene in more austral regions of Argentina.","palabras_clave":"CINGULATA|FORMACION LUMBRERA|DASYPODIDAE|NOROESTE ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5312,"idioma_id":4,"titulo":"Host suitability of peppers to the false root-knot nematode Nacobbus aberrans","resumen":"The false root-knot nematode, Nacobbus aberrans, causes severe damage to field and greenhouse pepper crops in several localities of the American continent. No commercial peppers (Capsicum annuum) resistant to this nematode are available up to the present. Host suitability of 6 experimental and 3 commercial peppers (some of them carrying Me1 and Me7 resistance genes to Meloidogyne spp.) to two N. aberrans populations were evaluated under greenhouse experiments. None of the peppers was found to be resistant to the nematode. The evaluated parameters exhibited significant differences among some peppers tested within a single population and between populations for a single plant material. Somepeppers carrying resistance showed higher nematode reproduction than some lines that did not possess resistance genes. These results confirmed that the genes conferring resistance to Meloidogyne spp. do not provide protection against this species of root-galling nematode. Host suitability of pepper lines carrying Me1 or Me7 resistance genes against N. aberrans is evaluated for the first time. Search for resistant genes against this nematode in wild peppers growing in areas where this nematode is indigenous should be promoted.","palabras_clave":"Capsicum annuum|False root-knot nematode|resistance genes|Nematode reproduction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5313,"idioma_id":4,"titulo":"Alpha6-beta\f4- Integrin Genetic Variations (A380T and R1281W) and Breast Cancer Risk in an Argentinian Population","resumen":"The 64 integrin is composed of the 6 and 4 subunits that are encoded by the ITGa6 and the ITGb4 genes, respectively. The 64 main function is to intervene in lamination and epithelia integrity maintenance by cell-matrix interactions. This integrin appears to have importance in breast cancer malignancy, as well as other epithelial carcinomas. The aim of this work was to investigate thepotential role of ITGa6 (A380T) and ITGb4 (R1281W) genetic variations in breast cancer susceptibility, in a female population from the northeast region of Argentina (Misiones). We performed a case-control study of 85 breast cancer patients and 113 cancer-free controls. Genotyping was performed by RFLP-PCR. For ITGa6 (A380T) single nucleotide polymorphism, a high frequency of heterozygousgenotype GA in cases compared to controls was observed, achieving values of 48% and 49%, respectively. No association between the A380T SNP and breast cancer development was found (Odds Ratio = 0.92; 95% Confidence Interval = 0.52?1.63; p = 0.884). In conclusion, we did not find evidence of an association between A380T (ITGa6) and the risk of developing  breast cancer.The results represent the first report of these genetic variations in breast cancer; therefore, they are an important contribution to the literature.","palabras_clave":"BREAST CANCER|GENETIC VARIATION|INTEGRINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5314,"idioma_id":4,"titulo":"Kernel setting at the apical and sub-apical ear of older and newer Argentinean maize hybrids","resumen":"Rainfed maize (Zea mays, L.) crops in temperate semi-arid regions with high inter-annual variation of summer precipitation, are commonly cultivated at low population densities. During seasons with favorable conditions for plant growth (e.g., summer rainfalls above normal records), the number of kernels of sub-apical ear can contribute significantly to total kernel number per plant (TKN). However, there is no information of the determinant traits of kernel setting at sub-apical ear, or the effects of breeding on these traits. We used a crop physiology model with an individual plant approach in attempt to describe genotypic differences in those traits related to kernel setting at the apical (KNE1) and the sub-apical ear (KNE2) of older and newer Argentinean maize hybrids. Four representative hybrids of the decades of 80&#8242;, 90&#8242;, 00&#8242; and 10&#8242; were cultivated during two growing seasons at three densities (4, 8 and 12 pl m&#8722;2). Nondestructive techniques were used to estimate the growth rate of individual plant (PGRPC), apical (E1GRCP) and sub-apical (E2GRCP) ear during the period bracketing silking (critical period), biomass partitioning to reproductive sinks (E1GRCP PGRCP&#8722;1 and E2GRCP PGRCP&#8722;1) and the efficiency to set kernels of the plant (TKN PGRCP&#8722;1) and the ears (KNE1 E1GRCP&#8722;1 and KNE2 E2GRCP&#8722;1). Differences among tested hybrids in KNE1 (ca. 400?600 k ear&#8722;1) were mainly determined by KNE1 E1GRCP&#8722;1 (ca. 230?280 k d&#8722;1 g&#8722;1) while newer hybrids had the highest E1GRCP PGRCP&#8722;1 (>0.50). By contrast, hybrids had similar KNE2 (ca. 124 k ear&#8722;1) due to their similar E2GRCP PGRCP&#8722;1 (ca. 0.08) and KNE2 E2GRCP&#8722;1 (ca. 93 k d&#8722;1 g&#8722;1). These results suggest that breeding effect on TKN was mainly determined by KNE1 E1GRCP&#8722;1 and for the newest hybrid this trait did not interact with plant density. Hence, the newest hybrid exhibited a positive linear response of TKN to PGRPC (r2 = 0.82) reaching the highest TKN among hybrids at low, mid and high density. A better performance of maize crops at low densities could be obtained by breeding for E2GRCP PGRCP&#8722;1.","palabras_clave":"MAIZE|KERNEL NUMBER|APICAL EAR|SUB-APICAL EAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5315,"idioma_id":4,"titulo":"Heat Stress during Grain Filling Modifies Kernel Protein Composition in Field-Grown Maize","resumen":"Episodes of extremely high temperatures (>35ºC) may cause a premature cessation of maize kernel growth (i.e. heat stress), depressing crop grain yield. However, little is known about the influence of this constraint on chemical composition of maize kernels, a key trait for end-use related attributes. Four maize genotypes (flint, popcorn, temperate semi-dent and temperate×tropical semi-dent) with distinctive endosperm types were grown at heated and non-heated temperature regimes during early or late stages of the effective grain-filling period. Heat stress during early stages decreased both protein and starch contents of kernels, but the impact on the former was lower (up to -42%) than on the latter (up to -50%), resulting in increases of kernel protein concentration (up to +15%). The flint and popcorn hybrids, with hard endosperm type, tended to be less vulnerable to such effects due to an enhanced capacity to sustain kernel growth. For all hybrids, heat stress during late stages of kernel growth, reduced similarly protein and starch contents (up to -38%), without affecting kernel protein concentration. Heat stress always altered endosperm protein composition by increasing the relative abundance of glutelins, and &#946;- plus &#947;-zeins, at the expense of that of &#945;-zeins. The significant environment × endosperm-type interaction for kernel chemical compounds should be linked to quality parameters for different maize end-uses.","palabras_clave":"MAIZE|HEAT STRESS|PROTEIN|ZEINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5316,"idioma_id":4,"titulo":"Influence of the rearing system on yolk corticosterone concentration in captive Greater Rheas (Rhea americana)","resumen":"Many environmental conditions elevate plasmacorticosterone in laying birds, leading to elevated hormone accumulation in theegg. We investigated whether maternal yolk corticosterone levels in GreaterRheas differ between fresh eggs collected from an intensive (IRS) and asemi-extensive (SRS) rearing system. After HPLC validation, yolk corticosteronewas measured using a corticosterone 125I radio-immunoassay kit. Results(mean ± SE) showed that eggs collected from the IRS exhibited a significantly highercorticosterone concentration than eggs from SRS (89.88 ± 8.93 vs. 45.41 ± 5.48ng\/g yolk, respectively). Our findings suggest that rearing conditions under anintensive scheme (e.g. small pens with bare ground, no direct foraging and handling)might be perceived as more stressful for Greater Rhea females than semi-extensiverearing conditions (e.g. low animal density distributed in extensive areas anddirect foraging), which would result in the transfer of higher yolkcorticosterone levels. A better understanding of environmental conditions andfemale traits that affect yolk corticosterone deposition provides a background for futures studies concerning the roles ofmaternal corticosterone on offspring development. ","palabras_clave":"corticosterone|avian physiology|huevo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5317,"idioma_id":4,"titulo":"'High resolution, week-long, locomotion time series from Japanese quail in a home-box environment'","resumen":"Temporal and spatial patterns of locomotion reflect both resting periods and the movement from one place to another to satisfy physiological and behavioural needs. Locomotion is studied in diverse areas of biology such as chronobiology and physiology, as well as in biomathematics. Herein, the locomotion of 24 visually-isolated Japanese quails in their home-box environment was recorded continuously over a 6.5 days at a 0.5&#8201;s sampling rate. Three time series are presented for each bird: (1) locomotor activity, (2) distance ambulated, and (3) zone of the box where the bird is located. These high resolution, week-long, time series consisting of 1.07×106 data points represent, to our knowledge, a unique data set in animal behavior, and are publically available on FigShare. The data obtained can be used for analyzing dynamic changes of daily or several day locomotion patterns, or for comparison with existing or future data sets or mathematical models across different taxa.","palabras_clave":"animal behavior|power law|wavelet analysis|time series"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5318,"idioma_id":4,"titulo":"Genome-wide association mapping of quantitative traits in a breeding population of sugarcane","resumen":"Background: Molecular markers associated with relevant agronomic traits could significantly reduce the time andcost involved in developing new sugarcane varieties. Previous sugarcane genome-wide association analyses (GWAS)have found few molecular markers associated with relevant traits at plant-cane stage. The aim of this study was toestablish an appropriate GWAS to find molecular markers associated with yield related traits consistent acrossharvesting seasons in a breeding population. Sugarcane clones were genotyped with DArT (Diversity ArrayTechnology) and TRAP (Target Region Amplified Polymorphism) markers, and evaluated for cane yield (CY) andsugar content (SC) at two locations during three successive crop cycles. GWAS mapping was applied within a novelmixed-model framework accounting for population structure with Principal Component Analysis scores as randomcomponent.Results: A total of 43 markers significantly associated with CY in plant-cane, 42 in first ratoon, and 41 in secondratoon were detected. Out of these markers, 20 were associated with CY in 2 years. Additionally, 38 significantassociations for SC were detected in plant-cane, 34 in first ratoon, and 47 in second ratoon. For SC, one marker-traitassociation was found significant for the 3 years of the study, while twelve markers presented association for2 years. In the multi-QTL model several markers with large allelic substitution effect were found. Sequences of fourDArT markers showed high similitude and e-value with coding sequences of Sorghum bicolor, confirming the highgene microlinearity between sorghum and sugarcane.Conclusions: In contrast with other sugarcane GWAS studies reported earlier, the novel methodology to analyzemulti-QTLs through successive crop cycles used in the present study allowed us to find several markers associatedwith relevant traits. Combining existing phenotypic trial data and genotypic DArT and TRAP markercharacterizations within a GWAS approach including population structure as random covariates may prove to behighly successful. Moreover, sequences of DArT marker associated with the traits of interest were aligned inchromosomal regions where sorghum QTLs has previously been reported. This approach could be a valuable toolto assist the improvement of sugarcane and better supply sugarcane demand that has been projected for theupcoming decades.","palabras_clave":"Biomass|Linkage disequilibrium|Population structure|Quantitative trait loci (QTL)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5319,"idioma_id":4,"titulo":"Use of Molecular Markers to Improve the Agro-Industrial Productivity in the North West of Argentina","resumen":"Genetic improvement of crop plants through conventionalbreeding has been the cornerstone to improve global agriculturalproduction. Nowadays, different molecular biology techniques havebecome available to supplement and assist conventional breeding,among them the utilization of molecular markers. The generation ofmolecular, or DNA, markers are based on two basic molecular biologymethods in order to be able to detect polymorphism: Southern blotting,a nucleic acid hybridization technique [1], and the amplification in vitroin vitroof specific DNA segments by the Polymerase Chain Reaction (PCR)technique [2]. By using these two methods and several variations of thebasic technologies, many different kind of molecular markers have beendeveloped during the last decades.","palabras_clave":"MOLECULAR MARKERS|PLANT BREEDING|CROP PRODUCTIVITY|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5320,"idioma_id":4,"titulo":"ENCRUSTING AND BORING BARNACLES THROUGH THE CRETACEOUS\/PALEOGENE BOUNDARY IN NORTHERN PATAGONIA (ARGENTINA)","resumen":"Information about barnacles as fossil components of hard substrate communities from middle latitudes in the Southern Hemisphere is scarce. Changes in these barnacle communities during episodes of extinction such as occurred during the Cretaceous\/Paleogene (K-Pg) boundary are almost unknown. We describe encrusting and boring barnacles associated with Maastrichtian and Danian oysters, evaluate taphonomic processes involved, and report changes in their frequencies over time. A total of 1,174 valves belonging to nine oyster species, collected from the Jagüel and Roca formations of the Neuquén Basin, were analyzed. Presence\/absence of barnacles or their bioerosional traces were recorded, frequencies of host incrustation and bioerosion were calculated, and taphonomic and statistical analyses were performed. Encrusting barnacles identified on the oyster shells were assigned to Verruca rocana Steinmann and their traces assigned to the ichnospecies Centrichnus concentricus Bromley and Martinell. Boring barnacles are represented by traces assigned to the ichnogenus Rogerella De Saint-Seine. A preliminary taphonomic analysis indicated that late Maastrichtian and early Danian shells showed fair-poor condition by abrasion and fragmentation as taphonomic attributes, while late Danian shells exhibited mostly good-fair or mixed condition for both attributes. Verruca rocana shows no records during late Maastrichtian but high frequencies of encrusted valves after the K-Pg boundary. Boring barnacles represented by the trace Rogerella exhibited a decline in abundance during early Danian but an increase at late Danian. Both encrusting and boring barnacles associated with oysters presented an increase in theirfrequencies during late Danian.","palabras_clave":"Encrusting|Borings|Barnacle|Cretaceous\/Paleogene"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5321,"idioma_id":4,"titulo":"Genetic Evidence of Expansion by Passive Transport of Aedes (Stegomyia) aegypti in Eastern Argentina","resumen":"Ver archivo adjunto","palabras_clave":"Aedes aegypti|Dispersal|Dengue|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5322,"idioma_id":4,"titulo":"In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots","resumen":"Azospirillum brasilense is a soil bacterium capable of promoting plant growth. Several surface components were previously reported to be involved in the attachment of A. brasilense to root plants. Among these components are the exopolysaccharide (EPS), lipopolysaccharide (LPS) and the polar flagellum. Flagellin from polar flagellum is glycosylated and it was suggested that genes involved in such a posttranslational modification are the same ones involved in the biosynthesis of sugars present in the O-antigen of the LPS. In this work, we report on the characterization of two homologs present in A. brasilense Cd, to the well characterized flagellin modification genes, flmA and flmB, from Aeromonas caviae. We show that mutations in either flmA or flmB genes of A. brasilense resulted in non-motile cells due to alterations in the polar flagellum assembly. Moreover, these mutations also affected the capability of A. brasilense cells to adsorb to maize roots and to produce LPS and EPS. By generating a mutant containing the polar flagellum affected in their rotation, we show the importance of the bacterial motility for the early colonization of maize roots.","palabras_clave":"Motility|Root-adsorption|Lipopolysaccharides|Exopolysaccharides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5323,"idioma_id":4,"titulo":"Long-term follow-up of essential thrombocythemia patients treated with anagrelide: subgroup analysis according to JAK2\/CALR\/MPL mutational status.","resumen":"BACKGROUND:Anagrelide represents a treatment option for essential thrombocythemia, although its place in therapy remains controversial.AIM:To assess the impact of mutational status in response rates and development of adverse events during long-term use of anagrelide.METHODS:We retrospectively evaluated 67 patients with essential thrombocythemia treated with anagrelide during 68 (4-176) months.RESULTS:Mutational frequencies were 46.3%, 28.3% and 1.5% for JAK2V617F, CALR and MPL mutations. Anagrelide yielded a high rate of hematologic responses, which were complete in 49.25% and partial in 46.25%, without differences among molecular subsets. The rate of thrombosis during treatment was 1 per 100 patient-years, without excess bleeding. Anemia was the major adverse event, 30.3% at 5-year follow-up, being more frequent in CALR+ (p60 months were at higher risk, OR (95%CI) 9.32 (1.1-78.5), p<0.01, indicating the need for bone marrow monitoring during prolonged treatment.CONCLUSION:Although CALR+ patients were at higher risk of developing anemia, anagrelide proved effective among all molecular subsets, indicating that mutational status does not seem to represent a major determinant of choice of cytoreductive treatment among essential","palabras_clave":"ANAGRELIDE|JAK2|CALR|TROMBOCITEMIA ESENCIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5324,"idioma_id":4,"titulo":"Tectonic models for the Patagonian orogenic curve (southernmost Andes): an appraisal based on analog experiments from the Fuegian thrust-fold belt","resumen":"Tectonic models for the evolution of the Patagonian orogenic curve were evaluated using analog experimentsthat considered either a rotational or a non-rotational orogenic backstop, combinedwith a basement promontoryon the foreland cratonic margin. Five different kinematic configurations were used, aiming to evaluate theinfluence of the Río Chico Arc as a rigid obstacle on the evolution of the Fuegian thrust?fold belt. Rotations, strainsand displacement fields obtained from each analog experiment were compared with the structural geologyknown from the Fuegian thrust?fold belt, in order to appraise the tectonic models that are more consistentwith the natural structure. The push of a counterclockwise rotational backstop, combined with the buttressingeffect of a foreland promontory, seem of major importance in controlling the final structure and map-viewshape of the thrust wedge.","palabras_clave":"Analog modeling|Patagonian orocline|Fuegian Andes|Rotational backstop|Basement promontory|Buttressing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5325,"idioma_id":4,"titulo":"Evolutionary history of the little fire ant Wasmannia auropunctata before global invasion: inferring dispersal patterns, niche requirements and past and present distribution within its native range","resumen":"The evolutionary history of invasive species within their native range mayinvolve key processes that allow them to colonize new habitats. Therefore,phylogeographic studies of invasive species within their native ranges areuseful to understand invasion biology in an evolutionary context. Here weintegrated classical and Bayesian phylogeographic methods using mitochondrialand nuclear DNA markers with a palaeodistribution modellingapproach, to infer the phylogeographic history of the invasive ant Wasmanniaauropunctata across its native distribution in South America. We discussour results in the context of the recent establishment of this mostly tropicalspecies in the Mediterranean region. Our Bayesian phylogeographic analysissuggests that the common ancestor of the two main clades of W. auropunctataoccurred in central Brazil during the Pliocene. Clade A would have differentiated northward and clade B southward, followed by a secondarycontact beginning about 380 000 years ago in central South America. Therewere differences in the most suitable habitats among clades when consideringthree distinct climatic periods, suggesting that genetic differentiation wasaccompanied by changes in niche requirements, clade A being a tropical lineageand clade B a subtropical and temperate lineage. Only clade B reachedmore southern latitudes, with a colder climate than that of northern SouthAmerica. This is concordant with the adaptation of this originally tropicalant species to temperate climates prior to its successful establishment in theMediterranean region. This study highlights the usefulness of exploring theevolutionary history of invasive species within their native ranges to betterunderstand biological invasions.","palabras_clave":"Bayesian phylogeography|biological invasion|invasive ant|mitochondrial DNA|Pleistocene refugia|range expansion|South America|species distribution model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5326,"idioma_id":4,"titulo":"Detection of pathogenic leptospiral DNA traces in canine sera serum samples by Loop Mediated Isothermal Amplification (LAMP)","resumen":"Leptospirosis is a worldwide distributed zoonosis, caused by pathogenic strains of spirochetes belonging to the genus Leptospira spp. So far, the only gold standard diagnostic method for this disease has been the Micro-Agglutination Test (MAT). The purpose of this study was to test whether a correlation exists between serological diagnosis by MAT and the presence of Leptospira spp. DNA traces in serum. Anti-Leptospira spp. antibody titers were determined in dog serum samples obtained at a canine shelter from Buenos Aires Province, Argentina (n= 80) by MAT using ten Leptospira spp. serovars. DNA extracted from these samples using Chelex-100 resin was used as template for Leptospira spp. specific PCR and loop-mediated isothermal amplification (LAMP) tests. In the latter case, product detection by incubation with malachite green dye was set up, obtaining optimal results at a dye concentration of 0.02% (w(v)) and a pH of 6.1. PCR and LAMP showed the same sensitivity, detecting leptospiral DNA in 12 out of the 48 MAT- positive sera. No detection was attained by either method in MAT-negative samples. These results show that it is possible to use LAMP for the detection of leptospirosis in non-human mammals. It also shows that, in the case of serum samples, MAT has higher diagnostic sensitivity than the currently available PCR and LAMP tests.","palabras_clave":"Leptospirosis|Serum|LAMP|Diagnostics|Canine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5327,"idioma_id":5,"titulo":"Aborto por Leptospira en una yegua en Salta, Argentina","resumen":"La leptospirosis en equinos es generalmente asintomática, aunque existen regiones donde la enfermedad constituye un problema. Aquí se describe un caso de aborto en yegua debido a la infección por este agente. Se efectuó la necropsia completa de un feto a término donde se colectaron muestras para inmunofluorescencia directa (IFD), histopatología y microbiología. Además se efectuó un estudio serológico de la tropilla y se evaluó seroconversión en la yegua abortada. El feto evidenció hepatomegalia, esplenomegalia e ictericia. Microscópicamente se apreció hepatitis mononuclear con disociación de los hepatocitos, esplenitis aguda y glomérulo-nefritis. Aunque el microrganismo no pudo ser aislado, la enfermedad se confirmó por la seroconversión observada en la yegua abortada, y debido a la identificación del agente mediante IFD en la impronta renal. Este caso demuestra la presencia del agente localmente y evidencia que la enfermedad puede ser un problema para la producción ecuestre","palabras_clave":"leptospirosis |pérdidas reproductivas|aborto equino"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5328,"idioma_id":4,"titulo":"Covalent functionalization of single-walled carbon nanotubes with polytyrosine: Characterization and analytical applications for the sensitive quantification of polyphenols","resumen":"This work reports the synthesis and characterization of single-walled carbon nanotubes (SWCNT) covalently functionalized with polytyrosine (Polytyr); the critical analysis of the experimental conditions to obtain the efficient dispersion of the modified carbon nanotubes; and the analytical performance of glassy carbon electrodes (GCE) modified with the dispersion (GCE\/SWCNT-Polytyr) for the highly sensitive quantification of polyphenols. Under the optimal conditions, the calibration plot for the amperometric response of gallic acid (GA) shows a linear range between 5.0 × 10-7 and 1.7 × 10-4 M, with a sensitivity of (518 ± 5) m AM-1 cm-2, and a detection limit of 8.8 nM. The proposed sensor was successfully used for the determination of total polyphenolic content in tea extracts.","palabras_clave":"SINGLE-WALLED CARBON NANOTUBES|COVALENT FUNCTIONALIZATION|POLYTYROSINE|GALLIC ACID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5329,"idioma_id":4,"titulo":"Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors","resumen":"Wereport for the first timethe use of glassy carbon electrodes (GCE)modified by drop-coating with multi-walled carbon nanotubes (MWCNTs) dispersed in cytochrome c as platformfor the construction of bienzymatic biosensors, using the glycoenzyme glucose oxidase (GOx) as model. The sensitivity of the resulting biosensor at &#8722;0.050 V towards glucose using hydroquinone (H2Q) as redox mediator was (96 ± 2) &#956;A M&#8722;1, with a linearrange between 1.00 × 10&#8722;4 M and 1.00 × 10&#8722;3 M and a detection limit of 8 &#956;M. The biosensor demonstratedto be highly reproducible with RSD. values of 6.1% for five different bioelectrodes. The combination of the excellent dispersing properties of Cyt c, the stability ofMWCNTs-Cyt c dispersion and the intimate interaction between Cyt c, MWCNTs and GOx, allowed the construction of an efficient glucose biosensor successfully used to determine glucose in commercial beverages.","palabras_clave":"MULTI-WALLED CARBON NANOTUBES|CYTOCHROME|CNT FUNCTIONALIZATION|GLUCOSE OXIDASE|ELECTROCHEMICAL BIOSENSOR|BIENZYMATIC BIOSENSOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5330,"idioma_id":4,"titulo":"Single-walled carbon nanotubes covalently functionalized with polytyrosine:  a new material for the development of NADH-based biosensors","resumen":"We report for the first time the use of single-walled carbon nanotubes (SWCNT) covalently functionalized with polytyrosine (Polytyr) (SWCNT-Polytyr) as a new electrode material for the development of nicotinamide adenine dinucleotide (NADH)-based biosensors. The oxidation of glassy carbon electrodes (GCE) modified with SWCNT-Polytyr at potentials high enough to oxidize the tyrosine residues have allowed the electrooxidation of NADH at low potentials due to the catalytic activity of the quinones generated from the primary oxidation of tyrosine without any additional redox mediator. The amperometric detection of NADH at 0.200 V showed a sensitivity of (217 ± 3) &#956;A mM-1 cm-2 and a detection limit of 7.9 nM. The excellent electrocatalytic activity of SWCNT-Polytyr towards NADH oxidation has also made possible the development of a sensitive ethanol biosensor through the immobilization of alcohol dehydrogenase (ADH) via Nafion entrapment, with excellent analytical characteristics (sensitivity of (5.8 ± 0.1) &#956;A mM-1 cm-2, detection limit of 0.67 &#956;M) and very successful application for the quantification of ethanol in different commercial beverages.","palabras_clave":"COVALENTLY FUNCTIONALIZED CARBON NANOTUBES|POLYTYROSINE|ELECTROCHEMICAL ETHANOL BIOSENSOR|ELECTROCHEMICAL NADH SENSOR|NADH ELECTROCATALYTIC OXIDATION|ALCOHOL DEHYDROGENASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5331,"idioma_id":4,"titulo":"Nanoparticles and capillary electrophoresis: a marriage with  environmental impact","resumen":"The impact of nanomaterials in the environment and human health is a cause of big concern and even though intensive studies are currently being carried out, there is still a lot to elucidate. The development of validated methods for the characterization and quantification of nanomaterials and their impact on the environment should be encouraged to achieve a proper, safe and sustainable use of nanoparticles. Recently, capillary electrophoresis emerged as a well-adapted technique for the analysis of environmental samples. This review presents the application of nanoparticles together with capillary electrophoresis systems forenvironmental pollutants analysis, as well as the application of capillary electrophoresis techniques for the analysis of various types of nanoparticles.","palabras_clave":"Nanoparticles|capillary electrophoresis|environmental impact"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5332,"idioma_id":4,"titulo":"Iron buffer system in the water column and partitioning in the sediments of the naturally acidic Lake Caviahue, Neuquén, Argentina","resumen":"Sedimentary iron partitioning was studied for five sediment strata (16 cm depth) at three sampling sites of the naturally-occurring acidic Lake Caviahue (Patagonia, Argentina). Additionally, water column iron was modeled based on five-year period input loadings to study a possible iron buffer system. The partition coefficient between thewater column and the total iron content of the sedimentswas also addressed. Sedimentary ironwas found to be distributed, on average, in the following forms: exchangeable (6%), iron oxides (4%), pyrite and reactive organic matter (38%) and residual (non-andesitic) materials with a high content of humic acids (52%). Furthermore,we found that the dissolved iron in the lake was nearly constant throughout the five year period we studied. This is consistent with the existence of an iron buffer system in the lake at pH between 2.0 and 3.0, which may cause differential iron precipitation at the delta of the volcanic river with respect to the deeper northern and southern arms. Sedimentary iron measurements taken at the delta further support the existence of a buffer system, where it was found that the iron content in the sub-superficial stratum (2 cm) was double that of the remainder of the vertical profile at the same site.","palabras_clave":"ACID LAKE|IRON PARTITIONING|IRON MODELING|HUMIC ACIDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5333,"idioma_id":4,"titulo":"Volatile profile evolution of Reggianito cheese during ripening under different temperature-time combinations","resumen":"Using solid-phase microextraction and gaschromatography-mass spectrometry (SPME-GC-MS) procedure, volatile compoundswere studied during ripening of Reggianito cheeses stored under differenttemperature-time combinations. Volatile profile was characterised for cheesesripened under standard conditions at 0, 61, 124 and 180 days of ripening, andchanges occurring between 61 and 180 days were evaluated for cheeses in whichan elevated temperature of 20 °C was applied within the first month ofmaturation. A total of 41 volatile compounds including acids, ketones,aldehydes, esters, alcohols and hydrocarbons were identified. Despite thepresence of other compounds of potential relevance for cheese flavour, carboxylicacids clearly dominated the volatile profile of Reggianito cheeses. &nbsp;The more notable variations in the volatilefraction of Reggianito cheeses occurred during the first 2 months of ripeningand were mostly explained by changes observed for the predominant acid fraction.Significant changes in the quantities for some volatile compounds were observedfor the different temperature-time combinations applied, though the balancebetween the different groups of compounds was not considerably affected andsmall changes were observed for total chromatographic area values. In this work,a first description of changes occurring during ripening for the differentvolatile compounds present in traditional Reggianito cheese was provided.","palabras_clave":"REGGIANITO CHEESE|SOLID-PHASE MICROEXTRACTION AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY (SPME-GC-MS)|VOLATILE COMPOUNDS|RIPENING|ELEVATED TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5334,"idioma_id":4,"titulo":"Whey protein hydrolysis with free and immobilized Alcalase®: effects of operating parameters on the modulation of peptide profiles obtained","resumen":"In this work, the effects of operating parameterson the modulation of peptide profiles obtained during enzymatic hydrolysis of wheyprotein concentrates (WPC) with a commercial enzyme (Alcalase 2.4L AF) were analyzed.In this way, hydrolyzates of WPC were obtained working with free andimmobilized enzyme and evaluating different operating conditions (initialsubstrate concentrations, time, temperature and pH). Characteristicsof hydrolyzates obtained were evaluated by the hydrolysis degree obtained (DH) andthe peptide profile by RP-HPLC. Moreover, a quantitativedescriptive sensory analysis was performed to compare different preparations of commercial dessertsprepared using as ingredients milk, WPC and WPC hydrolyzates. The DHvalues increased very quickly during the first 10 min. followed by a slow increaseup to 25 min. For the range of conditions tested, we proposed to operate attemperatures close to 50&#730;C, pH between 8 and 9 and initial substrateconcentration from 7% as good reactions conditions. Finally, whey proteinhydrolyzates obtained with Alcalase showed very interesting sensorialcharacteristics to be incorporated in desserts commercial preparations","palabras_clave":"WHEY PROTEIN|ENZYMATIC HYDROLYSIS|ALCALASE|PEPTIDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5335,"idioma_id":4,"titulo":"Heterologous recombinant protein with decapacitating activity prevents and reverts cryodamage in ram sperm: An emerging biotechnological tool for cryobiology","resumen":"During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the freezing extenders and explore the beneficial and detrimental effects of seminal plasma or seminal plasma components in cryopreservation. Seminal plasma is known to contain stabilizing proteins, thereby this is a good start point to study the maintenance of membrane stability based on the basic knowledge of sperm physiology. However, seminal plasma composition is variable among rams and also the introduction of exogenous seminal plasma or its fractions to commercial semen can be associated with the transmission of viral diseases. Our work shows that a mouse protein, called SPINK3 (Serine Protease Inhibitor Kazal type 3) with decapacitating activity interacts with heterologous ram sperm when it is produced as a recombinant molecule. By immunocytochemistry assays we demonstrate that this protein (naturally expressed by mouse seminal vesicle under androgenic control) binds to the apical portion of both fresh and frozen ram sperm, the same localization described in mouse homologous sperm. Furthermore, it significantly improves sperm progressive motility compared to non-treated samples when it is added to freezing extenders and to dilution media after thawing. On the contrary, addition of SPINK3 does not modify sperm viability. The percentage of sperm with intact acrosome after ionophore induction was also significantly higher in sperm frozen in the presence of SPINK3 compared to control samples and the addition of SPINK3 after thawing significantly reduced both induced and non induced acrosomal loss, indicating that heterologous SPINK3 might act as a calcium inhibitor transport as described in mouse. Based on our results SPINK3 may find a place as a desirable biotechnological tool to achieve a higher proportion of competent sperm to fertilize.","palabras_clave":"RECOMBINANT SPINK3 |CRYOPRESERVATION|BIOTECHNOLOGY|RAM SPERMATOZOA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5337,"idioma_id":4,"titulo":"Hydrocarbons from bio-oils. Performance of the matrix in FCC catalysts in the immediate catalytic upgrading of different raw bio-oils","resumen":"Matrices of FCC catalysts with different content of alumina and the compound catalysts prepared with them including Y zeolite were used to upgrade bio-oils from pine wood sawdust and soybean shell. The biomasses were selected according to their different lignin content which results in very different proportions of phenolic compounds (coke precursors) in the respective bio-oils. The bio-oils were produced by fast pyrolysis and the vapours were immediately upgraded over a fixed bed of catalyst at550 C using a mass catalyst\/bio-oil relationship of 3.5. In terms of hydrocarbon yield, the matrices were more effective in deoxygenating pine wood sawdust bio-oil and the compound catalysts in deoxygenating soybean shell bio-oil. These differences can be the consequence of the different compositions of the bio-oils which, in the case of pine wood sawdust, includes a significant concentration of phenolic ethers which form coke on the matrix and the external surface of the zeolite, thus preventing lighter compounds from accessing the micropore system of the compound catalysts. Pine sawdust bio-oil produced more coke than soybean shell bio-oil; in the case of compound catalysts, coke deposited preferentially on the catalyst matrix, thus decreasing its mesopore specific surface area up to 65 %.","palabras_clave":"BIO-OIL|SILICA-ALUMINA|FUELS|FCC \u0002 |BIOMASS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5338,"idioma_id":5,"titulo":"Classification-based Mining of Reusable  Components on Software Product Lines (Indexed  SCI,  IF  JCR2014: 0.326)","resumen":"Software Product Lines and Component-based systems can be combined to maximize reuse in a predictable and opportunistic manner. When a product line is built for a certain subdomain within a more generic domain, future needs from a closely subdomain may be fulfilled by mining the line´s internal components to build a new product line. In this work, we present an approach to classify internal and external (third party) reusable components into a repository, by applying a K-Nearest Neighbors strategy, as a support for building new product lines. Natural language techniques and the WordNet lexical database are also used to process information from software components. We validate the approach with an experiment based in a dataset of external third-party components and reusable components from a product line that we built in the geographic subdomain of marine ecology.","palabras_clave":"Software Product Lines|Software Components|Software Reuse|K-Nearest Neighbors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5339,"idioma_id":4,"titulo":"DIVERSITY OF NECROPHAGOUS BLOWFLY (DIPTERA: CALLIPHORIDAE) OF MEDICAL AND VETERINARY IMPORTANCE IN URBAN ENVIRONMENTS IN CÓRDOBA (ARGENTINA)","resumen":"The complex nature of urban environments can have different effects on speciesdiversity and composition. The aim of this work was to characterize the assemblageof Calliphoridae regarding its richness, abundance, and synanthropy in CórdobaCity, Argentina. Three sampling sites differing in their distance to the border of thecity and degree of urbanization were selected. In each site, collections were carriedout with 12 traps baited with cow liver (200 g per trap) that were operated for fiveconsecutive days during three different times of the year, in April, June and August2013. A total of 341 adult calliphorids from nine species, Lucilia sericata (Meigen), L.eximia (Wiedemann), L. cuprina (Wiedemann), L. cluvia (Walker), Calliphora vicinaRobineau-Desvoidy, Sarconesia chlorogaster (Wiedemann), Chrysomya albiceps(Wiedemann), C. megacephala (Fabricius) and C. chloropyga (Wiedemann) werecollected. Lucilia sericata was the most abundant species followed by C. vicina.Species diversity, composition and abundance changed between sites, richness beinglowest at the most urbanized site. All species are cosmopolitan except Sarconesiachlorogaster, whose distribution is restricted to South America. These results areconsistent with a homogenization of the fauna in urban environments.","palabras_clave":"Diptera|Calliphoridae|Urban Landscape|Diversity."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5340,"idioma_id":4,"titulo":"High resolution images above the Pampean flat slab of Argentina (31-32ºS) from local receiver functions: Implications on regional tectonics","resumen":"In the flat slab region of the South Central Andes (~31-32ºS), geological observations suggest that the regional crustal structure is inherited from the accretion of different terranes during the Ordovician. These structures were later reactivated, first in extension during the Triassic and later in compression during the Andean uplift since the Miocene. Seismological observations confirmed that those fault structures extend to depth with décollement levels that accommodate current crustal shortening in the region. In order to get better insight on the regional tectonics we computed higher frequency receiver functions from local slab seismicity of intermediate ~100 km depth. Using a common conversion point (CCP) stacking method we obtained cross sections showing high vertical resolution crustal structure at the transition between the Precordillera and the Frontal Cordillera. In addition we performed a joint inversion of our high frequency receiver functions with surface wave data from ambient noise tomography allowing us to constrain absolute seismic wave velocities. Our higher resolution images reveal more structural details down to a depth of 80 km and laterally over the flat slab in good agreement with previous studies. Our results help to better identify very shallow discontinuities in seismic velocities. Recent petrological analyses combined with our RF high-resolution structure correlates with a crustal mafic composition and partial eclogitization in the lower crust. We observe a shift in the crustal structure between the Precordillera and the Frontal Cordillera. Regional seismicity and previously determined focal mechanisms superimposed over our images indicate this shifting is a thrust structure extending down to a depth of 40 km. Our results combined with petrological analyses suggest the presence of a master décollement between the Cuyania (Western Precordillera) and Chilenia (Frontal Cordillera) terranes that probably accommodates the deformation in the Pampean flat slab region since the Late Ordovician.","palabras_clave":"Terrane|Andean retroarc|Subduction|Body waves|Surface waves|Eclogite|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5341,"idioma_id":4,"titulo":"The role of propionibacteria in the volatile profile of Pategrás cheeses","resumen":"Pategrás is a typical semi-hard cheese of Argentina. Over the years, successive technological changes have been applied to its manufacture, being the addition of propionibacteria (PAB) one of the most relevant. The aims of the present work were to determine some physicochemical parameters and volatile composition of commercial samples and evaluate the effect of PAB on volatile profile during ripening, by comparing cheeses made at pilot scale, with or without added PAB. Commercial samples displayed some differences in terms of rough composition, mainly ripening degree. Acids, especially short-chain fatty acids (C2 to C4), were the most representative group of volatile compounds, being propanoic acid the most abundant. Alcohols and ketones were other chemical groups that prevailed in the volatile profiles whereas esters and aldehydes constituted a minority fraction. For cheeses made at pilot scale, the levels of several compounds such as diacetyl, acetoin, primary alcohols, ethyl esters, branched-chain aldehydes, branched-chain alcohols, branched-chain acids, and short-chain fatty acids were significantly influenced by the presence of PAB. Most of the changes observed in the volatile fraction during ripening and the differences found between cheeses made with and without added PAB were in agreement with published data. This work provides detailed information about the volatile composition of Pategrás cheeses made with the inclusion of PAB, which contribute to the characterization of the most traditional Argentinean semi-hard cheese.","palabras_clave":"Volatile profile|Physicochemical composition|Propionibacteria |Pategrás cheese"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5342,"idioma_id":4,"titulo":"PROP1 triggers epithelial-mesenchymal transition-like process in pituitary stem cells","resumen":"Mutations in PROP1 are the most common cause of hypopituitarism in humans; therefore, unraveling its mechanism of action is highly relevant from a therapeutic perspective. Our current understanding of the role of PROP1 in the pituitary gland is limited to the repression and activation of the pituitary transcription factor genes Hesx1 and Pou1f1, respectively. To elucidate the comprehensive PROP1-dependent gene regulatory network, we conducted genome wide analysis of PROP1 DNA binding and effects on gene expression in mutant mice, mouse isolated stem cells and engineered mouse cell lines. We determined that PROP1 is essential for stimulating stem cells to undergo an epithelial to mesenchymal transition-like process necessary for cell migration and differentiation. Genomic profiling reveals that PROP1 binds to genes expressed in epithelial cells like Claudin 23, and to EMT inducer genes like Zeb2, Notch2 and Gli2. Zeb2 activation appears to be a key step in the EMT process. Our findings identify PROP1 as a central transcriptional component of pituitary stem cell differentiation.","palabras_clave":"PROP1|STEM CELLS|EMT|HIPOPITUITARISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5343,"idioma_id":4,"titulo":"B-esterase determination and organophosphate insecticide inhibitory effects in JEG-3 trophoblasts","resumen":"The placenta and trophoblasts express several B-esterases. This family includes acethylcholinesterase (AChE), carboxylesterase (CES) and butyrylcholinesterase (BChE), which are important targets of organophosphate insecticide (OP) toxicity. To better understand OP effects on trophoblasts, B-esterase basal activity and kinetic behavior were studied in JEG-3 choriocarcinoma cell cultures. Effects of the OP azinphos-methyl (Am) and chlorpyrifos (Cp) on cellular enzyme activity were also evaluated.JEG-3 cells showed measurable activity levels of AChE and CES, while BChE was undetected. Recorded Km for AChE and CESwere 0.33 and 0.26mMrespectively. Native gel electrophoresis and RT-PCR analysis demonstrated CES1 and CES2 isoform expression. Cells exposed for 4 and 24 h to the OP Amor Cp, showed a differential CES and AChE inhibition profiles. Am inhibited CES and AChE at 4 h treatment while Cp showed the highest inhibition profile at 24 h. Interestingly, both insecticides differentially affected CES1 and CES2 activities. Results demonstrated that JEG-3 trophoblasts express AChE, CES1 and CES2. B-esterase enzymes were inhibited by in vitro OP exposure, indicating that JEG-3 cells metabolization capabilities include phase I enzymes, able to bioactivate OP. In addition, since CES enzymes are important for medicinal drug activation\/deactivation, OP exposure may interfere with trophoblast CES metabolization, probably being relevant in a co-exposure scenario during pregnancy.","palabras_clave":"ORGANOPHOSPHATE PESTICIDES|CHLORPYRIFOS|AZINPHOS-METHYL|ACETYLCHOLINESTERASE|CARBOXYLESTERASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5345,"idioma_id":4,"titulo":"Genetic Characterization and Gene Expression of Bile Salt Hydrolase (bsh ) from Lactobacillus Reuteri Crl 1098,a Probiotic Strain","resumen":"Intestinal microbes containing the bile salt hydrolase (BSH) enzyme, releases free BA plus aminoacids from conjugated BA.BSH activity triggerscholesterol consumption in liver to synthesize BA de novo leading to consequential cholesterol lowering.Lactobacillus(L.)reuteriCRL 1098 is a probiotic bacterium with a proven hypocholesterolemic effect associated to its ability tohydrolyze BA. In this work we characterized the bile salt hydrolase (bsh)operonof CRL 1098 strain as a single open readingframe of 978 nucleotides that encodesa predicted protein of 325 amino acids, with a calculated mass of 36098.1 Daand a theoretical pI of 4.81. Moreover, deduced BSH protein had high similarity with BSHs of other L. reuteri strain andalso exhibited similarity to the Pencillin V amidases of Listeria and Bacillus strains. Five catalytically important amino acidswere highly conserved in Lactobacillus, Enterococcus and Bifido bacterium strains while four amino acid motifs aroundthese active sites, were only partially conserved. After the bsh gene product was expressed in the heterologous host LactococcuslactisNZ9000.The activity was specific towards bile acids but not against alternative substrates. Finally, a significant upregulationof the bsh gene was observed at pH 5.2 (optimal pH of BSH activity).Our studies suggest that BSHs would have an important but so far unknown role in the physiology and lifestyle of L. reuteristrains. The present work would be useful to unravel the ecological role of the BSH and to deepen their influence in thereduction of blood cholesterol levels.","palabras_clave":"LACTOBACILLUS REUTERI|BILE SALT HYDROLASE|MOLECULAR CLONING|GENE EXPRESSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5346,"idioma_id":4,"titulo":"Antioxidant, anticancer activities and mechanistic studies of the flavone glycoside diosmin and its oxidovanadium(IV) complex. Interactions with bovine serum albumin","resumen":"The naturalantioxidant flavonoid diosmin, found in citric fruits, showed low antioxidantproperties among other flavonoids due to its structural characteristics and lowcytotoxicity against lung (A549) and breast (T47D, SKBR3 and MDAMB231) cancercell lines. The anticancer behavior has been improved by the metal complexgenerated with the flavonoid and the oxidovanadium(IV) ion. This new complex, [VO(dios)(OH)3]Na5.6H2O(VOdios), &nbsp;has been synthesized andcharacterized both in solid and solution states. The interaction of the metalion through the sugar moiety of diosmin precluded the improvement of theantioxidant effects. However, the cell-killing effects tested in human lungA549 and breast T47D, SKBR3 and MDAMB231 cancer cell lines, were enhanced bycomplexation. The anti-proliferative effects on the human lung cancer cell linewere accompanied by cellular ROS generation and an increase in cytoplasmcondensation. The breast cancer cell lines did not produce caspase3\/7activation, mitochondrial potential reduction and ROS generation. Therefore, a non-apoptoticform of cell death in a caspase- and oxidative stress- independent manner hasbeen proposed. The proteinbinding ability has been monitored by the quenching of tryptophan emission inthe presence of the compounds using bovine serum albumin (BSA) as a modelprotein. Both compounds could be distributed and transported in vivo and the complex displayed stronger binding affinity and higher contributionsto the hydrogen bond and van der Waals forces.","palabras_clave":"Diosmin|Oxidovanadium(IV) complexes|Antioxidant|Anticancer|BSA binding"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5347,"idioma_id":4,"titulo":"'PROPOSED MECHANISMS FOR OLIGONUCLEOTIDE IMT504 INDUCED DIABETES REVERSION IN A MOUSE MODEL OF IMMUNODEPENDENT DIABETES'","resumen":"Type 1 diabetes (T1D) originates from autoimmune &#946;-cell destruction. IMT504 is an immunomodulatory oligonucleotide that increases mesenchymal stem cells cloning capacity and reverts toxic diabetes in rats. Here we evaluated long-term (20 doses) and short-term (2-6 doses) effects of IMT504 (20 mg\/kg\/day, sc) in an immunodependent diabetes model: multiple low dose streptozotocin-injected BALB\/c mice (40 mg\/kg\/day, i.p. for 5 consecutive days). We determined blood glucose, glucose tolerance, serum insulin, islet morphology, islet infiltration, serum cytokines, progenitor cell markers, immunomodulatory proteins, proliferation, apoptosis, and islet gene expression. Long-term results: IMT504 reduced glycemia, induced &#946;-cell recovery and impaired islet infiltration. Short-term analysis: IMT504 induced early blood glucose decrease, infiltration inhibition, increased &#946;-cell proliferation and decreased apoptosis, increased islet indoleamine 2,3-dioxygenase (IDO) expression, increased serum tumor necrosis factor and interleukin-6 (IL-6). IMT504 affected islet gene expression: Preproinsulin-2, Proglucagon, Somatostatin, Nestin, Regenerating gene-1 and C-X-C motif ligand-1 cytokine (Cxcl1) increased in islets from diabetic mice and were decreased by IMT504. IMT504 downregulated Platelet endothelial cell adhesion molecule-1 (Pecam1) in islets from control and diabetic mice, whereas it increased Regenerating gene-2 (Reg2) in islets of diabetic mice. The IMT504-induced increase in IL-6 and islet IDO expression, and decreased islet Pecam1 and Cxcl1 mRNA expression could participate in keeping leukocyte infiltration at bay, whereas upregulation of Reg2 may mediate &#946;-cell regeneration. We conclude that IMT504 effectively reversed immunodependent diabetes in mice. Corroboration of these effects in a model of autoimmune diabetes more similar to human T1D could provide promising results for the treatment of this disease.","palabras_clave":"TYPE 1 DIABETES|OLIGONUCLEOTIDES|IMT504|BETA CELL REGENERATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5348,"idioma_id":4,"titulo":"Effect of the protein corona on the colloidal stability and reactivity of LDH-based nanocarriers","resumen":"The physicochemical properties of drug nanocarriers such as layered double hydroxide nanoparticles (LDH-NPs) determine their circulation times in biological media and their interaction with the targeted cells. Nevertheless, the components of the biological fluid, and particularly the formation of a protein corona, change the properties of as-prepared nanocarriers. Here, we discuss the effect of the protein corona formation on the colloidal stability and reactivity of LDH-NPs intercalated with chloride (LDH?Cl), carbonate (LDH?CO3) or dodecylsulfate (LDH?DS). These solids present model physicochemical properties (hydrophillic character, surface charge, and exchange capacity) that can be obtained depending on the interaction of drugs with LDH layers. The colloidal stability of LDH-NPs was determined in simulated biological fluids at high ionic strength and\/or the presence of albumin (the main protein of human blood plasma), whereas the reactivity was evaluated by dissolution kinetics in acidic media, compatible with the environment of cell internalized nanocarriers. The protein corona increased the colloidal stability of the nanocarriers by steric hindrance at high ionic strength, reverted the positive zeta potential of as-prepared LDH-NPs and protected them from dissolution at low pHs. The properties of the anionic cargo of LDH-NPs strongly affected the protein corona and hence the fate of NPs in biological fluids. Drug nano- carriers with interfacial properties similar to those of LDH?Cl and LDH?CO3 seem to be more promising than LDH?DS in forming a protein corona. Then, LDH?Cl and LDH?CO3 would enable long circulation times due to their size, colloidal stability and low protein damage. Our results indicate that LDH-NPs preserve and even improve their properties as drug nanocarriers after interacting with the biological media, particularly their ability to reach the site of therapeutic action from the injection place.Introduction","palabras_clave":"Layered double hydroxides|Protein Corona|Particle size|Adsorption"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5349,"idioma_id":4,"titulo":"A social-ecological network analysis of Argentinean Andes transhumant pastoralism","resumen":"Many mountainous regions worldwide arecharacterized by harsh environments, scarce infrastructure,and extreme contrasts between mountains and neighboringplateaus and plains. Transhumance is a social adaptation tohandle geomorphological heterogeneity such as lowlandsand highlands, and to cope with environmental variability(seasonal and regional rainfall and snowfall). We studiedthe regional transhumant system with a network approachin the Andes of North Patagonia, Argentina. We measuredthe connectivity promoted by the seasonal movements ofherds and people (relationships) among different ecosystems(nodes), defined as winter and summer lands. Weidentified 238 networks. The highest frequencies correspondedto small network structures (dyads and triads),suggesting that landscape management is highly decentralized.Network complexity was positively related toecological richness and diversity of connected nodes.However, most networks were dependent upon a centralnode, suggesting vulnerable situations regarding","palabras_clave":"CONNECTIVITY|MOBILITY|RESILIENCE|VARIABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5350,"idioma_id":4,"titulo":"Expression of a repressor form of the Arabidopsis thaliana transcription factor TCP16 induces the formation of ectopic meristems","resumen":"Plants that express a fusion of the Arabidopsis thaliana class I TCP transcription factor TCP16 to the EAR repressor domain develop several phenotypic alterations, including rounder leaves, short petioles and pedicels, and delayed elongation of sepals, petals and anthers. In addition, these plants develop lobed cotyledons and ectopic meristems. Ectopic meristems are formed on the adaxial side of cotyledon petioles and arise from a cleft that is formed at this site. Analysis of the expression of reporter genes indicated that meristem genes are reactivated at the site of emergence of ectopic meristems, located near the bifurcation of cotyledon veins. The plants also show increased transcript levels of the boundary-specific CUP-SHAPED COTYLEDON (CUC) genes. The results suggest that TCP16 is able to modulate the induction of meristematic programs and the differentiation state of plant cells.","palabras_clave":"TCP|meristema|genes CUC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5351,"idioma_id":5,"titulo":"Bases fisiológicas del crecimiento diferencial en la fase inicial del desarrollo de dos progenies de Pinus taeda bajo condiciones de campo, en Misiones Argentina","resumen":"Para contribuir al conocimiento de caracteres ecofisiológicos útiles en la selección de genotipos aptos para ciertas áreas ecológicas, en el contexto de cambio climático, el objetivo del presente trabajo fue evaluar variables morfo-fisiológicas responsables del crecimiento diferencial de plantines de Pinus taeda bajo condiciones ambientales subtropicales. Se evaluaron en medio-hermanos provenientes de una madre de crecimiento rápido (CR) y una de crecimiento lento (CL) las variables: incremento en altura, diámetro a la altura del cuello (DAC), conductancia estomática (gs), potencial hídrico (&#936;) mínimo y máximo, fotosíntesis neta a saturación de radiación fotosintéticamente activa (Asat), conductancia (Kh) y conductividad hidráulica específica (ks) y foliar específica (kl) de ramas, y asignación de biomasa aérea. Los genotipos CR presentaron mayor incremento en altura y DAC y mayor producción de biomasa aérea. Los patrones de gs, &#936; y Asat fueron similares entre genotipos. En contraste, los genotipos CR presentaron mayor eficiencia de conducción de agua por unidad de área foliar en condiciones de alta disponibilidad de agua, aunque su ks fue más afectada bajocondiciones de déficit hídrico que en los CL. Las diferencias observadas en asignación de biomasa aérea, que modifican la arquitectura hidráulica de la planta, serían parcialmente responsables de las tasas diferenciales de crecimiento. Asimismo, la tendencia de menor regulación estomática en los genotipos CR, reflejada en mayor grado de anisohidrismo y mayor caída en ks en períodos secos, sugiere que estos podrían ser más susceptibles al déficit hídrico que los CL, requiriéndose más estudios para poner a prueba esta hipótesis.","palabras_clave":"Variación intraespecífica|Asignación de biomasa aérea|Incremento|Arquitectura hidráulica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5352,"idioma_id":4,"titulo":"Maxiclavella gen. nov., comb. nov. and Isaclavella nasalis gen. nov. sp. nov (Siphonostomatoida: Lernaepodidae) parasites of Isacia conceptionis (Perciformes, Haemulidae) collected in northern Chile, with a key for Clavella branch genera","resumen":"A new genus, Maxiclavella n. gen., is propose to accommodate Clavella simplex which presents more simple characteristics than other Clavella sp. including the armature and segmentation of antennules, the endopod armature of antenna, and diminutive bulla. A new combination is thus proposed: Maxiclavella simplex. In addition, another new genus and species for the Clavella branch is described herein as Isaclavella nasalis, this inhabits the olfactory sacs, no other genus in the Clavella branch colonizes such a particular site. Several characteristics differentiate I. nasalis from all other Clavella branch genera, including antennae with exopods shorter than endopods, a large lobular projection and smaller subcircular projection at the base of the cephalothorax, a subspherical and flat bulla, mandibles without secondary dentition, and a suboval male body type. Males of both genera fit well in the Clavella branch male type, although they differ from each other in several ways (e.g. the position of buccal area, oriented ventrally in Maxiclavella while distally in Isaclavella, this last with caudal rami by the other hand absent in Maxiclavella). Molecular data confirm the validity of the new genera, which are presented in a new key to the Clavella branch genera. The Clavella branch include now 20 genera.","palabras_clave":"Maxiclavella|Chille|Isaclavella|key"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5354,"idioma_id":4,"titulo":"Electrochemical sensing of guanine, adenine and 8-hydroxy-2&#8242;-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine","resumen":"This work reports the synthesis and characterization of single-walled carbon nanotubes (SWCNT) covalently functionalized with l-lysine (Lys) and the analytical performance of glassy carbon electrodes (GCE) modified with a dispersion of SWCNT-Lys (GCE\/SWCNT-Lys) for the highly sensitive quantification of guanine, adenine and 8-hydroxy-2&#8242;-deoxyguanosine. Detection limits of 75, 195 and 97 nM were obtained for guanine, adenine and 8-hydroxy-2&#8242;-deoxyguanosine, respectively by voltammetric adsorptive stripping with medium exchange. GCE\/SWCNT-Lys was successfully used for the detection of adenine and guanine oxidation after adsorption of salmon sperm-double stranded DNA. A clear definition of 8-hydroxy-2&#8242;-deoxyguanosine oxidation signal is observed even in the presence of large excess of guanine, adenine or salmon sperm-double stranded DNA.","palabras_clave":"XX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5355,"idioma_id":4,"titulo":"Effects of different energy sources on cell adhesion and bioleaching of a chalcopyrite concentrate by extremophilic archaeon Acidianus copahuensis","resumen":"Bioleaching is an alternative technology for recovering metals from mineral ores; thermophilic microorganisms instead of mesophiles can greatly improve metal solubilization, particularly from refractory mineral species. A. copahuensis is a new species of thermophilic archaea, recently isolated in our laboratory. The effect of culture growth history on the attachment of A. copahuensis to a chalcopyrite concentrate was investigated in shake flasks at 65 °C. Cells adapted to growth with chalcopyrite as energy source showed higher attachment to the mineral concentrate. A. copahuensis cells reached 100 % of copper extraction in the bioleaching of chalcopyrite concentrate carried out in shake flasks cultures incubated at 65 °C and pH 2.0. This high bioleaching yield was achieved even at that not so low initial pH value probably because this archaeal species is able form sufficient amounts of ferric but keeping low redox potential (Eh) values. This work also describes the effect of the addition of other energy sources on the bioleaching activity. A. copahuensis achieved the best copper extraction without any addition but copper extraction decreased when alternative substrates were added.","palabras_clave":"BIOLEACHING|ACIDIANUS|COPPER|CHALCOPYRITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5356,"idioma_id":4,"titulo":"Biofilm formation and interspecies interactions in mixed cultures of thermo-acidophilic archaea Acidianus spp. and Sulfolobus metallicus","resumen":"The understanding of biofilm formation by metal-oxidizingmicroorganisms is of great importance for influencing mineral dissolutionrates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaeasuch as Acidianus, Sulfolobus and Metallosphaera are of special interestdue to their ability to perform leaching processes at high temperatures,thereby enhancing leaching rates. In this work, leaching experiments andvisualization by microscopy of cell attachment and biofilm formationpatterns of the crenarchaeotes Sulfolobus metallicus DSM 6482T and theAcidianus isolates DSM 29038 and DSM 29099 in pure and mixed cultures onsulfur or pyrite were studied. Confocal laser scanning microscopy (CLSM)combined with cell fluorescent dyes as well as fluorescently labeledlectins were used to visualize different components (e.g. DNA, proteinsor glycoconjugates) of the aforementioned species. The data indicate thatcell attachment and the subsequently formed biofilms were species- andsubstrate dependent. Pyrite leaching experiments coupled to precolonizationand further inoculation with a second species suggest thatboth species may negatively influence each other during pyrite leachingwith respect to initial attachment and pyrite dissolution rates. Inaddition, the investigation of binary biofilms on pyrite showed that bothspecies were heterogeneously distributed on pyrite surfaces in the formof individual cells or microcolonies. Physical contact between bothspecies seem to occur, as revealed by specific lectins able tospecifically bind single species within mixed cultures.","palabras_clave":"ACIDIANUS|SULFOLOBUS|BIOFILM|BIOLEACHING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5357,"idioma_id":4,"titulo":"LATE STAGE TRIFLUOROMETHYLTHIOLATION STRATEGIES FOR ORGANIC COMPOUNDS","resumen":"doi: 10.1039\/C6OB00763ESubstitution by the CF3S group allows for an increase in lipophilicity and electron-withdrawing properties along with an improvement in the bioavailability of medicinal targets; consequently, the late stage introduction of CF3S moieties into medicinal targets is a sought-after strategy in synthetic organic chemistry. Methods to accomplish such substitutions can be nucleophilic and electrophilic in nature. Different newly-developed electrophilic and nucleophilic reagents are used to effect the trifluoromethylthiolation of (hetero)aromatic compounds, aliphatic compounds (alkyl, alkenyl, alkynyl substrates), the trifluoromethylthiolation at the &#945;- and &#946;-carbonyl positions, and heteroatoms (N- and S-). Such reactions can involve homolytic substitutions, or functional-group substitutions (ipso). Addition reactions of electrophilic reagents to double and triple bonds followed by ring-cyclizations will be shown to yield relevant CF3S-substituted heteroaromatic compounds with relevant pharmacological action.","palabras_clave":"trifluoromethylthiolation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5358,"idioma_id":5,"titulo":"Hacia un aprendizaje significativo del Algebra Lineal mediante actividades vinculadas a proyectos extra aúlicos","resumen":"RESUMEN En el presente trabajo se presenta una propuesta de actividades integradas, donde se definen conceptos dentro de un ambiente contextual y se aborda una problemática de interés profesional, con el objetivo de favorecer el aprendizaje significativo del Algebra Lineal en alumnos universitarios. La propuesta trabaja el tema 'Reconocimiento de patrones de imágenes' de interés general para la Informática, Geofísica y Astronomía. La propuesta se pone en práctica, mediante una experiencia piloto con alumnos de la UNSJ, mostrando resultados alentadores y gratificantes tanto para los docentes como para los alumnos. ","palabras_clave":"aprendizaje significativo|algebra lineal|imágenes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5359,"idioma_id":5,"titulo":"Desarrollo embrionario-larval y metamorfosis de Physalaemus albonotatus (Anura: Leptodactylidae)","resumen":"Los anuros poseen una gran plasticidad fenotípica y constituyen un grupo de referencia para estudios de desarrollo ontogenético abarcando aspectos morfo-fisiológicos así como también enfoques ecológicos, filogenéticos y evolutivos. Existe abundante información sobre la morfología larval de numerosas especies de anuros, sin embargo, el desarrollo embrionario y larval ha sido escasamente explorado en Leptodactylidae. En el presente trabajo, se describen y caracterizan los principales cambios en la morfología externa de varios órganos así como también del sistema digestivo durante los estadios embrionarios, larvales y metamórficos de Physalaemus albonotatus. Para esto, se coleccionaron embriones y larvas en ambientes naturales que fueron criados en condiciones de laboratorio y fijados periódicamente en formol 10%. Las observaciones se realizaron bajo lupa estereoscópica y microscopio electrónico de barrido. El desarrollo embrionario y larval siguió el patrón característico de los anuros con fase larval acuática obligada. La organización de los principales órganos ocurrió durante los estadios embrionarios y primeros larvales, abarcando el periodo comprendido entre el estadio 17 y 25. Algunos órganos transitorios como las branquias externas y las papilas adhesivas desaparecen tempranamente durante el desarrollo, mientras que otras estructuras como  la cámara opercular, el espiráculo, el disco oral, el tubo proctodeal, la cola y las aletas caudales se conservan hasta el final del periodo larval, y se pierden recién durante la metamorfosis. En esta última etapa se evidencian cambios bruscos en la anatomía de la mayoría de los sistemas de órganos y se configura el diseño corporal característico del individuo juvenil. Las caracterizaciones morfológicas de estructuras embrionarias y larvales de Physalaemus albonotatus contribuirán con información de referencia aplicable a otros miembros de la familia Leptodactylidae.","palabras_clave":"ANFIBIOS|ONTOGENIA|ESTADIOS EMBRIONARIOS|MORFOLOGIA LARVAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5360,"idioma_id":4,"titulo":"High hydrostatic pressure assisted enzymatic hydrolysis of whey proteins","resumen":"Whey proteins, due to their high nutritional value, are generally hydrolyzed to reduce the allergenicity and used as ingredients in many special products, such as infant formulae, geriatric products, highly energetic supplements or dietetic foods or in foods produced to prevent nutrition related diseases, like food intolerances and allergies. The aimof this work was to assess the applicability of innovative technologies, such as high hydrostatic pressure (HHP) processes, to assist the enzymatic hydrolysis of target proteins, namely whey protein concentrate (WPC-80), in order to modify their antigenicity. Experiments were carried out to verify the effectiveness of HHP technology to accelerate whey protein hydrolysis reaction with selected enzymes (&#945;-chymotrypsin, bromelain),and to affect the protein allergenic power. To this purpose, different HHP treatments were carried out at several pressure levels (100, 200, 300 and 400 MPa) and the untreated whey protein samples were used as control. A de&#64257;ned enzyme\/substrate ratio of 1\/10 w\/w was used in the experiments, while the treatment time was changed from 0 to 30 min (0, 5, 15, or 30 min). The experimental data demonstrated that High Hydrostatic Pressure (HHP) induced WPC-80 unfolding at the highest value of the pressure applied (400 MPa) as indicated by the higher exposure of free sulfhydryl groups. When HHP was used in combination with enzymatic hydrolysis, the degree of hydrolysis increased not only with the pressure level applied but also with the processing time. These results suggested that, even if the exposure of hidden epitopes upon protein unfolding increased the antigenicity of whey proteins, further peptide bonds cleavage also took place after hydrolysis. This effect could modify whey proteins antigenic sequences, and thus their antigenic power.","palabras_clave":"HIGH HYDROSTATIC PRESSURE|ENZYMATIC HYDROLYSIS|FOOD ALLERGENS|WHEY PROTEINS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5361,"idioma_id":4,"titulo":"Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms","resumen":"Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5\/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8\/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.","palabras_clave":"cap|icaAD|CAPSULE|ADHESIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5362,"idioma_id":4,"titulo":"Effects of probiotics in swines growth performance: A meta-analysis of randomized controlled trials","resumen":"The objective of this meta-analysis was to assess effects of probiotics on the growth performance of pigs (Average Daily Gain (ADG) and Feed Efficiency (FE)). Data bases (ie. PubMed, ScienceDirect, and Scopus) were searched from 1980 to 2015 unrestricted by language. The inclusion criteria were: randomised and controlled experiments using pigs without apparent disease and published in peer reviewed journals. Sixty-seven and 60 experiments were included to assess probiotic effects on ADG and FE, respectively. LAB supplementation increased ADG (difference in mean (DM) = 29.930 g\/day, 95% confidence interval (CI) 17.617 to 42.261) and improve feed efficiency (DM = &#8722;0.096 kg feed\/kg body weight, 95%CI &#8722;0.120-0.071), considering the source of heterogeneity. There were no evidence of publication biases. The meta-analysis showed that application of probiotics during the first stage of pig grown and in the finishing period resulted in greater ADG and FE. The effect was not related to the use of mono-strain or multi-strain probiotics, although it may depend on the strain used. The breeds (especially F1 and three-breed-rotational crossbreeding) and the characteristic of these breeds (maternal breeds) included in the experiments had an impact on the outcomes. These results might be used to define the guidelines to standardize the experimental designs of future trials and to include the impact of each covariate on the differences in the estimated effect sizes.","palabras_clave":"PROBIOTIC|LACTIC ACID BACTERIA|SWINE|META-ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5363,"idioma_id":4,"titulo":"Poultry blood from slaughterhouses: development of a biopreservation system to improve microbiological quality prior to transforming it into by-products","resumen":"1. The aim of this study was to investigate the use of indigenous lactic acid bacteria (LAB) with specific additives as a Biopreservation System (BS) for poultry blood during its storage in slaughterhouses. 2. The BS consisted of two LAB (Enterococcus faecalis DSPV 008SA or Lactobacillus salivarius DSPV 032SA) with 4 additives (lactose 2 g\/l, yeast extract 0.4 g\/l, ammonium citrate 0.4 g\/l and NaCl 1 g\/l). After 24 h storage at 30oC, lower counts of enterobacteria, coliforms, Pseudomonas spp. and Staphylococcus aureus were evident in blood treated with the BS than in untreated blood.3. The ability of LAB to prevent haemolysis was evident. A decrease in pH was associated with control of spoilage microorganisms but it needed to be regulated to prevent coagulation of proteins.4. On the basis of these results it is recommended to supplement blood with a BS to avoid undesirable changes during blood storage before processing.","palabras_clave":"Poultry blood|biopreservation|microbiological quality|by-products"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5364,"idioma_id":4,"titulo":"Noise on resistive switching: a Fokker-Planck approach","resumen":"We study the effect of internal and external noise on the phenomenon of resistive switching. We consider a non-harmonic external driving signal and provide a theoretical framework to explain the observed behavior in terms of the related Fokker?Planck equations. It is found that internal noise causes an enhancement of the resistive contrast and that noise proves to be advantageous when considering short driving pulses. In the case of external noise, however, noise only has the effect of degrading the resistive contrast. Furthermore, we find a relationship between the noise amplitude and the driving signal pulsewidth that constrains the persistence of the resistive state. In particular, results suggest that strong and short driving pulses favor a longer persistence time, an observation that may find applications in the field of high-integration high-speed resistive memory devices.","palabras_clave":"stochastic processes (theory)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5365,"idioma_id":4,"titulo":"Evolution of morphologies of a PE-b-PEO block copolymer in an epoxy solvent induced by polymerization followed by crystallization-driven self-assembly of PE blocks during cooling","resumen":"Polymerization-induced nanostructuration combined with crystallization-driven self-assembly was used togenerate complex nanostructures in an epoxy network. A PE-b-PEO block copolymer (Mn ¼ 1400; 50 wt%PEO), was dispersed in diglycidylether of bisphenol A (DGEBA) and homopolymerization initiated bya tertiary amine was carried out at 120 C (above the melting temperature of PE). The plasticizationproduced by the miscible PEO blocks decreased the Tg of the cured matrix to values located below thecrystallization temperature of PE. Therefore, crystallization-driven self-assembly of PE blocks took placeduring the cooling step through the rubbery region of the epoxy network. Depending on the initialamount of PE-b-PEO dispersed in DGEBA, a variety of nanostructures could be generated, such asa dispersion of disk-like micelles (6.7 nm in thickness), a concentrated dispersion of short nanoribbons(50?200 nm in length and 6.7 nm in thickness), partially stacked and oriented in space, and complexspherulitic structures composed of large stacked nanoribbons. The thickness of micellar objects wasclose to the theoretical value of fully extended PE chains of the block copolymer. IR spectroscopyconfirmed the all-trans conformation of PE chains. Therefore, crystals were formed by interdigitated PEchains, with PEO blocks tethered at both planar interfaces in an alternating way. The way in which thesecomplex nanostructures affect the fracture resistance or functional properties (such as shape memory)of the resulting epoxy networks has yet to be analyzed.","palabras_clave":"Block copolymer|Epoxy|Self-assembly"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5366,"idioma_id":5,"titulo":"Un problema social. La trama del flagelo","resumen":"El consumo de drogas resulta una temática compleja cuyo análisis supone considerar la multiplicidad de formas que pueden asumir las significaciones sociales que se ponen en juego, las prácticas individuales y colectivas, sujetos, sustancias, y contextos socioeconómicos, políticos, culturales e ideológicos en los que dicho consumo se lleva a cabo. Para abordar este tema partimos de una perspectiva relacional, es decir, 'la droga' no existe como algo independiente de las variadas formas de su uso, las cuales no siempre responden al estereotipo que circula en los discursos formales (como el jurídico y el sanitario) y en los del sentido común (el mediático, por ejemplo). Hay diversos modos de consumo, que comprenden las frecuencias, cantidades y sustancias, pero también el tipo de compromiso en el uso definido por un sistema de relaciones y sus rituales alrededor de la toma del producto.","palabras_clave":"CONSUMOS PROBLEMÁTICOS DE DROGAS|VULNERABILIDAD SOCIAL|JUVENTUDES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5367,"idioma_id":4,"titulo":"The amphiphilic alkyl ester derivatives of L-ascorbic acid induce reorganization of phospholipid vesicles","resumen":"L-ascorbic acid alkyl esters (ASCn) are lipophilic forms of vitamin C, which maintain some of its antioxidant power. Those properties make this drug family attractive to be used in pharmacological preparations protecting other redox-sensible drugs or designed to reduce possible toxic oxidative processes. In this work, we tested the ability of L-ascorbic acid alkyl esters (ASCn) to modulate the structure, permeability and rheological properties of phospholipid bilayers. The ASCn studied here (ASC16, ASC14 and ASC12) alter the structural integrity as well as the rheological properties of phospholipid membranes without showing any evident detergent activity. ASC14 appeared as the most efficient drug in destabilize the membrane structure of nano- and micro-size phospholipid liposomes inducing vesicle content leakage and shape elongation on giant unilamellar vesicles. It also was the most potent enhancer of membrane microviscosity and surface water structuring. Only ASC16 induced the formation of drug-enriched condensed domains after its incorporation into the lipid bilayer, while ASC12 appeared as the less membrane-disturbing compound, likely because of its poor, and more superficial, partition into the membrane. We also found that incorporation of ASCn into the lipid bilayers enhanced the reduction of membrane components, compared with soluble Vitamin C. Our study shows that ASCn compounds, which vary in the length of the acyl chain, show different effects on phospholipid vesicles used as biomembrane models. Those variances may account for subtly differences in the effectiveness on their pharmacological applications.","palabras_clave":"GUVs|LAURDAN GP|DPH ANISOTROPY|CONFOCAL MICROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5368,"idioma_id":5,"titulo":"DETERMINACIÓN DE LA CONCENTRACIÓN DE GLIFOSATO EN AGUA MEDIANTE  LA TÉCNICA DE INMUNOABSORCIÓN LIGADA A ENZIMAS (ELISA)","resumen":"Los herbicidas constituyen fuentes potenciales decontaminación, siendo el agua uno de los componentes del ambiente másvulnerables. El glifosato (N-fosfonometil glicina) es el herbicida másutilizado a escala mundial. Actualmente, la información referida a su efectosobre el agua resulta insuficiente, dado fundamentalmente por los altos costosde los análisis de laboratorio. Los objetivos del presente trabajo fueron evaluarel empleo de la técnica de ELISA como una herramienta rápida para el monitoreo de la calidad deaguas y cuantificar la concentración de glifosato por medio de la técnica deHPLC-MS\/MS. Los resultados muestran que cuando la técnica de ELISA registra la presenciade glifosato en muestras de agua, se requiere la cuantificación de dichaconcentración mediante la técnica de HPLC-MS\/MS, para determinar la concentración exacta de glifosato endichas muestras. Los resultados sugierenque la técnica de ELISA, destacada por su simplicidad, bajocosto y sensibilidad, es factible de serimplementada en programas de monitoreo de aguas, constituyendo un métodoexploratorio que permite detectar cualitativamente la presencia o ausencia delherbicida glifosato. En todos los casos, cuando esta técnica detecta glifosato,se requiere determinar su concentración por HPLC-MS\/MS, como técnica deestándar de oro.","palabras_clave":"CONTAMINACION|AGUA|GLIFOSATO|ELISA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5369,"idioma_id":4,"titulo":"An ILP based heuristic for a generalization of the post-enrollment course timetabling problem","resumen":"We consider a new timetabling problem arising from a real-world application in a private university in Buenos Aires, Argentina. In this paper we describe the problem in detail, which generalizes the Post-Enrollment Course Timetabling Problem (PECTP), propose an ILP model and a heuristic approach based on this formulation. This algorithm has been implemented and tested on instances obtained from real data, showing that the approach is feasible in practice and produces good quality solutions.","palabras_clave":"INTEGER PROGRAMMING|MATHEURISTIC|UNIVERSITY TIMETABLING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5370,"idioma_id":4,"titulo":"Cohesive properties of (Cu,Ni)-(In,Sn) intermetallics: Database, electron-density correlations and interpretation of bonding trends","resumen":"Thispaper presents a systematic and comparative study of the composition and volumedependence of the cohesive properties for a large group of Me-X intermetallicphases (IPs) with Me=Cu,Ni and X=In,Sn, which are of interest in relation withthe design of lead-free soldering (LFS) alloys. The work relies upon a databasewith total-energy versus volumeinformation developed by using projected augmented waves (PAW) calculations. Inprevious papers by the current authors it was shown that these results accountsatisfactorily for the direct and indirect experimental data available. In thepresent work, the database is further expanded to investigate the compositiondependence of the volume (Vo), and the composition and volumedependence of the bulk modulus (Bo) and cohesive energy (Ecoh).On these bases, ananalysis is performed of the systematic effects of replacing Cu by Ni inseveral Me-X phases (Me=Cu,Ni and X=In,Sn) reported as stable and metastable,as well as various hypothetical compounds involved in the thermodynamicmodeling of IPs using the Compound-Energy Formalism. Moreover, it is shown thatthe cohesion-related quantities (Bo\/Vo)½ and(Ecoh½\/Vo) can be correlated with a parameterexpressing the number of valence electrons per unit volume. These findings arecompared in detail with related relations based on the Miedema empiricalelectron density at the boundary of the Wigner-Seitz cell. In view of theco-variation of the cohesive properties, Ecoh is selected as a keyproperty and its composition and structure dependence is examined in terms of atheoretical view of the bonding which involves the hybridization of the d-states of Cu or Ni with the s and p-states of In or Sn, for this class of compounds. In particular, acomparative analysis is performed of the DOS of various representative,iso-structural Me-X compounds. Various effects of relevance to understand theconsequences of replacing Cu by Ni in LFS alloys are highlighted and explainedmicroscopically for the first time.","palabras_clave":"INTERMETALLICS COMPOUNDS|AB INITIO CALCULATIONS|ELECTRONIC STRUCUTRE|THERMODYNAMIC PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5371,"idioma_id":4,"titulo":"Engineers as teachers. Notes to think about teacher training in the engineering context","resumen":"Integration of teachers into the institutional culture of each establishment is a bit worked area by the management of each The renewed labour market demands, with the requirements arising from the procedures of accreditation of undergraduate, locate professionals who serve as university professors, in the difficult crossroad to update their teaching profile without neglecting their disciplinal knowledge. On the other hand, the selection of professionals to integrate the university teaching staff rarely consider teaching skills as a criterion, although different programs and proposals aimed at meeting this demand are evident. It is known that the handling of disciplinary knowledge by teachers not guarantee results in student learning. Often teaching tasks are performed intuitively through the repetition of a model recovered from the evocation of personal experience as a student or trying to avoid in teaching activity those experiences that are meant as negative in their own learning. The training of engineers is not exempt from this issue, so this proposal aims to analyze the characteristics of teacher training at the Faculty of Engineering of the National University of San Juan","palabras_clave":"Teacher training|Engineering|Teaching"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5372,"idioma_id":4,"titulo":"Petrology and mineralogy of the La Peña igneous complex, Mendoza, Argentina: An alkaline occurrence in the Miocene magmatism of the Southern Central Andes","resumen":"The La Pe&ntilde;a alkaline igneous complex (LPC) is located in the Precordillera (32&deg;41´34&Prime; S - 68&deg;59´48&Prime; W) of Mendoza province, Argentina, above the southern boundary of the present-day flat-slab segment. It is a 19 km2 and 5 km diameter subcircular massif emplaced during the Miocene (19 Ma) in the Silurian-Devonian Villavicencio Fm. The LPC is composed of several plutonic and subvolcanic intrusions represented by: a cumulate of clinopyroxenite intruded by mafic dikes and pegmatitic gabbroic dikes, isolated bodies of malignite, a central intrusive syenite that develops a wide magmatic breccia in the contact with clinopyroxenite, syenitic and trachytic porphyries, a system of radial and ring dikes of different compositions (trachyte, syenite, phonolite, alkaline lamprophyre, tephrite), and late mafic breccias. The main minerals that form the LPC, ordered according to their abundance, are: pyroxene (diopside, hedenbergite), calcium amphibole (pargasite, ferro-pargasite, potassic-ferro-pargasite, potassic-hastingsite, magnesio-hastingsite, hastingsite, potassic-ferro-ferri-sadanagaite), trioctahedral micas (annite-phlogopite series), plagioclase (bytownite to oligoclase), K-feldspar (sanidine and orthoclase), nepheline, sodalite, apatite group minerals (fluorapatite, hydroxylapatite), andradite, titanite, magnetite, spinel, ilmenite, and several Cu-Fe sulfides. Late hydrothermal minerals are represented by zeolites (scolecite, thomsonite-Ca), epidote, calcite and chlorite.The trace element patterns, coupled with published data on Sr-Nd-Pb isotopes, suggest that the primary magma of the LPC was generated in an initially depleted but later enriched lithospheric mantle formed mainly by a metasomatized spinel lherzolite, and that this magmatism has a subduction-related signature. The trace elements pattern of these alkaline rocks is similar to other Miocene calc-alkaline occurrences from the magmatic arc of the Southern Central Andes.Mineral and whole-rock chemical compositions support the interpretation that a first batch of a tephritic magma produced a cumulate of clinopyroxenite (clinopyroxene + magnetite + apatite) and a residual melt that crystallized as malignite at a shallow emplacement level (&lt;5 km). Fractional crystallization in a deep chamber, coupled with rock assimilation, produced successive magma pulses that gave the composite central syenite, the syenitic and trachitic porphyries, and trachytic dikes. The latest rocks (phonolites) reflect an extreme fractionation with Ca, Al and K removal by feldspars. Mingling relationships between tephrites-basanites and trachytes-phonolites, plus compositional variations linked to the reabsorption surface observed in the K-feldspar from the phonolitic dikes, suggest recharge events with local mixing in the late-stages of the LPC evolution.The emplacement of the LPC melts at a shallow crustal level was favored by the aperture of extensional NNW-SSE fractures genetically linked to a local brittle shear zone, active at 18-19 Ma during a strong compressional event, that initiated the regime of flat-slab subduction in this part of the Andes.","palabras_clave":"Alkaline |Potassic|Miocene|Central Andes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5373,"idioma_id":4,"titulo":"PETROGRAPHY, MINERALOGY AND ORIGIN OF THE SYENITE PEGMATITIC SEGREGATION VEINS FROM LA PEÑA ALKALINE COMPLEX, MENDOZA, ARGENTINA","resumen":"The syenite pegmatitic segregation veins (SPSV) from the Early Miocene La Peña alkaline complex (LPC), Mendoza, Argentina, are emplaced in the internal unit of a malignite body. They occur as veins, parallel layers and small elliptical bodies 1 to 6 cm wide and 0.2 to 1 m in length. The veins with a pegmatitic to micropegmatitic texture have a thin dark border composed of andradite, potassic-hastingsite and dark micas and a thicker internal zone of poikilitic Ba+Sr-poor K-feldspar (Or90.3Ab8.9An0.8), nepheline (Ne74.9Ks19.4Qz5.7 to Ne78.7Ks20.0Qz1.3), potassic-hastingsite, dark mica, andradite, scarce clinopyroxene (diopside-hedenbergite) and locally zoned microphenocrysts of Ba+Sr rich K-feldspar (Or75.6Ab21.7An2.7). Accessory fluorapatite, titanite, interstitial sodalite and magnetite and secondary abundant Na- and K-rich zeolites, calcite, chlorite-group minerals and possibly cancrinite were identified.The textural and mineralogical characteristics of the SPSV show genetic links with the host malignite that belongs to the potassic alkaline series. The SPSV were formed from a residual syenitic melt (malignite) extracted from a parental tephritic magma. In the residual malignite, the concentration of primary crystals (apatite, clinopyroxene, Ba+Sr2 rich K-feldspar and nepheline) formed a crystal network (mush) with the open spaces occupied by small amounts of residual melt enriched in Al, Na, K and volatiles and depleted in Ba and Sr. The deformation (by shear movements plus magmatic pressures) produced microfractures and fractures, which were occupied by residual interstitial melt. Its in situ crystallization within these fractures resulted in the formation of the SPSV.","palabras_clave":"pegmatitic segregation|malignite|potassic alkaline rocks|interstitial melt|La Peña complex|Mendoza|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5374,"idioma_id":4,"titulo":"Extreme events in La Plata basin: A retrospective analysis of what we have learned during CLARIS-LPB project","resumen":"Extreme climate events over the La Plata basin (LPB) can produce significant impacts due to the importance of the regional agriculture and hydroelectric power production for the local economy. Progress on describing, projecting and understanding extremes in LPB, in the framework of the CLARIS-LPB Europe-South America Network for Climate Change Assessment and Impact Studies in La Plata Basin Project, are reviewed. The paper is based on recent studies and  publications, as well as some new diagnostics as indicators of works in progress, and can be considered as an update for the LPB region of previous reviews by Cavalcanti et al. (2015; J Hydrol 523:211?230) and Rusticucci (2012; Atmos Res 106:1?17). Despite the significant advances on regional extremes, somegaps have been identified, and many challenges remain. Much of the recent progress considers temperature and precipitation extremes on timescales varying from synoptic to long-term variability and climate change, essential for impact and vulnerability assessments. Research lines on extremes requiring further efforts include the relative roles of local versus remote forcings, the impact of land use and land management changes, the specific role of soil moisture and land-atmosphere feedbacks as catalysts for heat waves, the impact of the local inhomogeneities in soil moisture, feedback and uncertainties in projections of extremes, as well as seasonal forecast and climate change attribution studies. We suggest combining intensive monitoring and modelling to address these issues.","palabras_clave":"TEMPERATURE EXTREMES|PRECIPITATION EXTREMES|LA PLATA BASIN|CLIMATE MODELS|REGIONAL CLIMATE CHANGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5375,"idioma_id":4,"titulo":"Analysis of the coding-complete genomic sequence of groundnut ringspot virus suggests a common ancestor with tomato chlorotic spot virus","resumen":"Groundnut ringspot virus (GRSV) and tomato chlorotic spot virus (TCSV) share biological and serological properties, so their identification is carried out by molecular methods. Their genomes consist of three segmented RNAs: L, M and S. The finding of a reassortant between these two viruses may complicate correct virus identification and requires the characterization of the complete genome. Therefore, we present for the first time the complete sequences of all the genes encoded by a GRSV isolate. The high level of sequence similarity between GRSV and TCSV (over 90 % identity) observed in the genes and proteins encoded in the M RNA support previous results indicating that these viruses probably have a common ancestor","palabras_clave":"Groundnut ringspot virus|Complete sequence|Virus|Peanut"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5376,"idioma_id":4,"titulo":"Institutional culture and new teachers' integration. Report of an experience at High School","resumen":"Integration of teachers into the institutional culture of each establishment is a bit worked area by the management of each institution. It is known that when a new member comes in the institution, in general regulations are distributed, but there are certain practical rituals and knowledge, which the new teacher must learn through the gaps that are generated in everyday life. These processes usually happen spontaneously and are rarely formalized by institutional management. In teacher training, institutional culture is a widely discussed content; however, it is possible to notice a gap between the training approach and practical dilemmas that new teachers must meet in their first work experiences within educational institutions. This report attempts to describe and analyze the process of integration into the institutional culture of teachers at a High School institution in San Juan (Argentina), recovering the historical perspective and the current situation. Some strategies and alternatives for the integration of new teachers at institutional culture are also presented","palabras_clave":"Teacher training|Institutional culture|High school"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5377,"idioma_id":4,"titulo":"A cluster-first route-second approach for the swap body vehicle routing problem","resumen":"The swap body vehicle routing problem (SB-VRP) is a generalization of the classical vehicle routing problem where a particular structure as well as several operational aspects for the trucks composing the fleet are considered. This research has been motivated by the VeRoLog Solver Challenge 2014, organized together by VeRoLog and PTV group, aiming to motivate the study of real-world logistic problems. A truck can carry either only one swap body or, in addition, an extra trailer with an extra swap body. For the latter, special depots, called swap locations, can be used to drop and pickup the swap bodies. These operations may affect the feasibility and the cost of a route, and therefore the overall operational cost. In this paper, we propose a cluster-first route-second heuristic for the SB-VRP. Computational experiments are conducted over the benchmark instances proposed for the competition, simulating a practical environment by considering limited resources and execution time. The results obtained are of very good quality, where our approach ended as runner-up in the final set of instances and performs similarly to the other algorithms in the remaining cases, showing its potential to be applied in practice.","palabras_clave":"SWAP'BODY VRP|ITERATED LOCAL SEARCH|GRASP|CLUSTER-FIRST ROUTE-SECOND"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5378,"idioma_id":4,"titulo":"Generalized error-dependent prediction uncertainty in multivariate calibration","resumen":"Most of the current expressions used to calculate figures of merit in multivariate calibration have beenderived assuming independent and identically distributed (iid) measurement errors. However, it is wellknown that this condition is not always valid for real data sets, where the existence of many externalfactors can lead to correlated and\/or heteroscedastic noise structures. In this report, the influence of thedeviations from the classical iid paradigm is analyzed in the context of error propagation theory. Newexpressions have been derived to calculate sample dependent prediction standard errors under differentscenarios. These expressions allow for a quantitative study of the influence of the different sources ofinstrumental error affecting the system under analysis. Significant differences are observed when theprediction error is estimated in each of the studied scenarios using the most popular first-ordermultivariate algorithms, under both simulated and experimental conditions.","palabras_clave":"Multivariate analysis|Error structure|Prediction uncertainty|Partial least squares"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5379,"idioma_id":4,"titulo":"A new and consistent parameter for measuring the quality of multivariate analytical methods: generalized analytical sensitivity","resumen":"Generalized analytical sensitivity (g) is proposed as a new figure of merit, which can be estimated from a multivariate calibration data set. It can be confidently applied to compare different calibration methodologies, and helps to solve literature inconsistencies on the relationship between classical sensitivity and prediction error. In contrast to the classical plain sensitivity, g incorporates the noise properties in its definition, and its inverse is well correlated with root mean square errors of prediction in the presence of general noise structures. The proposal is supported by studying simulated and experimental first-order multivariate calibration systems with various models, namely multiple linear regression, principal component regression (PCR) and maximum likelihood PCR (MLPCR). The simulations included instrumental noise of different types: independently and identically distributed (iid), correlated (pink) and proportional noise, while the experimental data carried noise which is clearly non-iid.&nbsp;","palabras_clave":"Química Analítica|Espectroscopía|Quimiometría"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5380,"idioma_id":4,"titulo":"Assesment and academic trajectories in engineers training","resumen":"Assesment is an important part of the curriculum processes that strains the educational system. Assesment practices at university are a problem that is crossed by different factors among it highlights the institutional context and the pedagogical-didactic teachers? decisions. This work constitutes the progress of an ongoing descriptive and exploratory investigation, which focuses its attention on understanding the impact of the assesment system of learning in the academic careers of engineering students from the National University of San Juan. The conclusions, provisional yet since this is an ongoing investigation, are a valuable input for curriculum design and development at the School of Engineering, as a starting point for strengthening assesment processes, enabling joint practices, economy of efforts and quality assurance assesment at the institutional level. It tries also to be a contribution to making decisions, widely discussed in academic and institutional context, by providing a survey of the experiences around assesment processes, that teachers of the School of Engineering are making through their pedagogical practices in different degree courses. This research will also contribute to improve the quality of training of engineers that develops in the institutional context, as it could affect in increasing student retention rate and shortening the actual duration of the careers, in which assesment regimes have an important role.","palabras_clave":"Assessment|Curriculum|Engineers training"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5381,"idioma_id":5,"titulo":"Residuos de plaguicidas organofosforados y carbamatos en aguas subterráneas de bebida en las zonas rurales de Plottier y Senillosa, Patagonia Norte, Argentina","resumen":"Plottier y Senillosa son dos localidades contiguas que forman parte del área de producción agrícola del Alto Valle deRío Negro y Neuquén, Patagonia Norte. Entre los plaguicidas más utilizados predominan las familias de neonicotinoides, carbamatosy organofosforados. El conocimiento de los niveles de estos compuestos en aguas superficiales y subterráneas se haconvertido en un tema de interés social debido a su posible impacto en el ambiente y la salud. El objetivo del presente trabajo fuedeterminar niveles de residuos de plaguicidas en agua subterránea utilizada para bebida en zonas rurales de Plottier y Senillosa.Se analizaron carbamatos: propoxur, carbofuran, pirimicarb, carbaril y organofosforados: clorpirifos, dimetoato, metidation, fenaminfos,triazofos, fosmet y metil azinfos, por cromatografía gaseosa (GC), en seis sitios de muestreo, durante la época de aplicacióny no aplicación de insecticidas. No se detectaron residuos de los plaguicidas estudiados por el método analítico utilizado.El límite de detección fue 0,010 µg\/L para organofosforados y 0,030 µg\/L para carbamatos. Estos resultados indican que lasbuenas prácticas agrícolas aplicadas en la zona así como las características fisicoquímicas del suelo, su espesor y el contenidode materia orgánica y las propiedades fisicoquímicas de los plaguicidas estudiados son factores que contribuyen favorablementea la calidad del agua de bebida de la población rural.","palabras_clave":"PLAGUICIDAS|ORGANOFOSFORADOS|AGUAS SUBTERRANEAS|PATAGONIA NORTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5382,"idioma_id":4,"titulo":"Neopterin acts as an endogenous cognitive enhancer","resumen":"Neopterin is found at increased levels in biological fluids from individuals with inflammatory disorders. The biological role of this pteridine remains undefined; however, due to its capacity to increase hemeoxygenase-1 content, it has been proposed as a protective agent during cellular stress. Therefore, we investigated the effects of neopterin on motor, emotional and memory functions. To address this question, neopterin (0.4 and\/or 4pmol) was injected intracerebroventricularly before or after the training sessions of step-down inhibitory avoidance and fear conditioning tasks, respectively. Memory-related behaviors were assessed in Swiss and C57BL\/6 mice, as well as in Wistar rats. Moreover, the putative effects of neopterin on motor and anxiety-related parameters were addressed in the open field and elevated plus-maze tasks. The effects of neopterin on cognitive performance were also investigated after intraperitoneal lipopolysaccharide (LPS) administration (0.33mg\/kg) in interleukin-10 knockout mice (IL-10(-\/-)). It was consistently observed across rodent species that neopterin facilitated aversive memory acquisition by increasing the latency to step-down in the inhibitory avoidance task. This effect was related to a reduced threshold to generate the hippocampal long-term potentiation (LTP) process, and reduced IL-6 brain levels after the LPS challenge. However, neopterin administration after acquisition did not alter the consolidation of fear memories, neither motor nor anxiety-related parameters. Altogether, neopterin facilitated cognitive processes, probably by inducing an antioxidant\/anti-inflammatory state, and by facilitating LTP generation. To our knowledge, this is the first evidence showing the cognitive enhancer property of neopterin","palabras_clave":"memory|neopterin|learning|neuroinflammation|hippocampus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5383,"idioma_id":4,"titulo":"Immunomodulatory effect of baculovirus in chickens: How it modifies the immune response against infectious bursal disease virus","resumen":"Several reports have shown that baculoviruses (BVs) have strong adjuvant properties on the mammalian immune system. Recent studies of our group demonstrated the ability of BV to stimulate the innate immunity in chickens. In this investigation, we aimed to assess the potential antiviral effect of BV given both, before and after infectious bursal disease virus (IBDV). In the first case, specific pathogen free chickens were intravenously inoculated with 5 × 107 pfu of Autographa californica nuclear polyhedrosis virus and 3 h later were orally administered 2.5 × 105 egg infectious doses 50 of IBDV. In the second case, chickens received IBDV 3 h before BV inoculation. Five days later, chickens were bled and euthanized. RNA from the bursa was analyzed for cytokine production. Also, bursae were used for virus recovery, and processed for lymphocyte isolation. The results showed that the administration of BV 3 h after the inoculation with IBDV produced important changes in the effect that IBDV causes in the bursa. BV reduced the infiltration of T lymphocytes, decreased the expression pattern of IL-6 and IFN-&#947; and inhibited IBDV replication. The results herein presented demonstrate that this Lepidopteran virus shows antiviral activity in chickens under experimental conditions. Investigations under field conditions have to be done to probe this strategy as a valuable sanitary tool for the treatment and prevention of chicken diseases.","palabras_clave":"AVIAN INNATE IMMUNITY |BACULOVIRUS|INFECTIOUS BURSAL DISEASE VIRUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5384,"idioma_id":4,"titulo":"Distribution of high Zn concentration in unvegetated and Typha domingensis vegetated sediments","resumen":"An experiment was carried out to determine if the presence of Typha domingensis affects the accumulation and speciation of high concentrations of Zn in sediments of aquatic systems. Reactors containing sediment and 2 plants of T. domingensis were disposed by triplicate. The treatments were: 1) 100 mg L-1 Zn (Zn100); 2) 500 mg L-1 Zn (Zn500) and 3) Control, without Zn. The same treatments without plants were disposed. The experiment lasted 30 days. Zn was efficiently removed from water in all treatments (greater than 75%). In Zn500 treatments, Zn removal was significantly high in vegetated reactors. Zn accumulation was significantly high in the superficial sediment layer (0-3 cm). T. domingensis influenced Zn accumulation and its distribution in sediments. The unvegetated sediment accumulated higher Zn concentrations than vegetated sediments. Zn was accumulated in less available fractions in unvegetated than vegetated sediments. Submerged parts of leaves accumulated higher Zn concentration than the other plant organs. Plants did not show toxic symptoms, so it could be assumed that they would continue taking up Zn. In the occasion of a dump of high Zinc concentration, constructed wetlands could retain it, minimizing environmental impact. Further investigation is needed to evaluate the role of macrophytes in the sustainability of Zn retention in constructed wetlands.","palabras_clave":"Phytoremediation|Speciation|Macrophytes|Sediment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5385,"idioma_id":4,"titulo":"First report of Papaya Ring Spot virus infecting  Carica Papaya in Argentina","resumen":"Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5?-TCCAAGAATGAAGCTGTGGACGCTGGTT-3? and BoCP_Rev 5?-TYAGTTGCGCATACCCAGGAGAGAGT-3?. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.","palabras_clave":"Papaya ringspot virus|Carica papaya"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5386,"idioma_id":4,"titulo":"The ecohydrological imprint of deforestation in the semiarid Chaco: insights from the last forest remnants of a highly cultivated landscape","resumen":"The semiarid Chaco plains present one of the highest rates of forest clearing and agricultural expansion of the world. In other semiarid plains, such massive vegetation replacements initiated a groundwater recharge and salt mobilization process that, after decades, raised regional water tables and salts to the surface, degrading agricultural and natural ecosystems. Indirect evidence suggests that this process (known as dryland salinity) began in the Chaco plains. Multiple approaches (deep soil profiles, geoelectric surveys and monitoring of groundwater salinity, level and isotopic composition) were combined to assess the dryland salinity status in one of the oldest and most active agricultural hotspots of the region, where isolated forest remnants occupy an extremely flat cultivated matrix. Full vadose moisture and chloride profiles from paired agriculture-forest stands (17 profiles, six sites) revealed the following: a generalized onset of deep drainage with cultivation (32 to >87 mm year&#8722;1), full leaching of native chloride pools (13.7 ± 2.5 kg m&#8722;2) down to the water table after >40 years following clearing and differential groundwater table rises (0.7 to 2 m shallower water tables under agriculture than under neighbouring forests). Continuous level monitoring showed abrupt water table rises under annual crops (up to 2.6 m in 15 days) not seen under forests or pastures. Varying deep drainage rates and groundwater isotopic composition under agricultural plots suggest that these pulses are strongly modulated by crop choices and sequences. In contrast to other dryland salinity-affected areas of the world, forest remnants in the study area (10?20% of the area) are not only surviving the observed hydrological shifts but also sustaining active salty groundwater transpirative discharge, as evidenced by continuous water table records. The overall impact of these forest remnants on lowering neighbouring water tables would be limited by the low hydraulic conductivity of the sediments. As highly cultivated areas of the Chaco evolve to new hydrological conditions of shallower saline water tables, innovative crop rotations that minimize recharge, enhance transpirative discharge and tolerate salinity will be needed.","palabras_clave":"DRYLAND SALINITY|DEEP DRAINAGE|GROUNDWATER RECHARGE|LAND CLEARING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5388,"idioma_id":4,"titulo":"EVOLUTION OF THE HYDROGEN-BONDING MOTIF IN THE MELAMINE-CYANURIC ACID CO-CRYSTAL. A TOPOLOGICAL STUDY","resumen":"The melamine (M)\/cyanuric acid (CA) supramolecular system is perhaps one of the most exploited in the field of self-assembly because of the high complementarity of the components. However, it is necessary to investigate further the factors involved in the assembly process. In this study, we analyzed a set of 13 Mn\/CAm clusters (with n , m = 1, 2, 3), taken from crystallographic data, to characterize the nature of the hydrogen bonds involved in the self-assembly of these components as well as to provide greater understanding of the phenomenon. The calculations were performed at the B3LYP\/6-311++G(d,p) and &#969;-B97XD (single point) levels of theory, and the interactions were analyzed within the framework of the quantum theory of atoms in molecules and by means of molecular electrostatic potential maps. Our results show that the stablest structure is the rosette-type motif and the aggregation mechanism is governed by a combination of cooperative and anticooperative effects. Our topological resultsexplain the polymorphism in the self-assembly of coadsorbed monolayers of M and CA.","palabras_clave":"Supramolecular|Self-assembly|Rosette motif|Hydrogen bonds|Negative cooperativity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5391,"idioma_id":4,"titulo":"Silver-functionalized methyl-silica hybrid materials as antibacterial coatings on surgical-grade stainless steel","resumen":"Development of new materials enriched with noble metal ions, such as silver, cobalt or copper, is of great interest in the field of commercial and medical products due to its action against fungus and bacteria. Although a large number of products are chemically active against microorganisms, they are usually limited to fabrics for medical bandages and sportswear in order to avoid, respectively, infections and odors. On the other hand, sol-gel technology becomes the preparation of hybrid materials possible with introduction of different components, as inorganic salts or organic additives within its structure bringing up the possibility to develop functional materials for different purposes.In this work, the development of an antibacterial coating for metallic substrates is presented. The coating consists in a hybrid organic-inorganic sol-gel material doped with silver. The relation between the structure of the hybrid matrix, aggregation state of silver ions, and biocide behavior is analyzed as a function of the densifying thermal treatment. Sol-gel materials were synthesized through hydrolytic condensation, in acidic conditions, of tetraethoxysilane (TEOS) and methyl-triethoxysilane (MTES). Silica nanoparticles were added in order to give a mechanical reinforcement and silver nitrate as the supplier of Ag+ ions. Coatings were deposited on 316L stainless steel and microscope glass slides by the dip-coating process at a constant withdrawal rate and densified at 50, 150 and 450 °C. X-ray photoelectron spectroscopy, small angle X-ray spectroscopy and electrochemical impedance spectroscopy were used as characterization techniques in order to elucidate silver aggregation and its influence on the structural evolution of the hybrid matrix, as a function of the thermal treatment. At the same time, the biocide behavior, as a function of silver state, particles size and thermal treatment, was studied by inhibitory halo identification on Escherichia-coli cultures in agar diffusion tests.","palabras_clave":"SOL-GEL|SILVER NANOPÂRTICLES|EIS |SYNCHROTRON SAXS |XPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5392,"idioma_id":4,"titulo":"New Windows based Color Morphological Operators for Biomedical Image Processing","resumen":"Morphological image processing is well known as an efficient methodology for image processing and computer vision. With the wide use of color in many areas, the interest on the color perception and processing has been growing rapidly. Many models have been proposed to extend morphological operators to the field of color images, dealing with some new problems not present previously in the binary and gray level contexts. These solutions usually deal with the lattice structure of the color space, or provide it with total orders, to be able to define basic operators with required properties. In this work we propose a new locally defined ordering, in the context of window based morphological operators, for the definition of erosions-like and dilation-like operators, which provides the same desired properties expected from color morphology, avoiding some of the drawbacks of the prior approaches. Experimental results show that the proposed color operators can be efficiently used for color image processing.","palabras_clave":"COLOR MATHEMATICAL MORPHOLOGY|BIOMEDICAL IMAGE|ORDER|COMPLETE LATTICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5393,"idioma_id":4,"titulo":"Processing of microCT implant-bone systems images using Fuzzy Mathematical Morphology","resumen":"The relationship between a metallic implant and the existing bone in a surgical permanent prosthesis is of great importance since the fixation and osseointegration of the system leads to the failure or success of the surgery. Micro Computed Tomography is a technique that helps to visualize the structure of the bone. In this study, the microCT is used to analyze implant-bone systems images. However, one of the problems presented in the reconstruction of these images is the effect of the iron based implants, with a halo or fluorescence scattering distorting the micro CT image and leading to bad 3D reconstructions. In this work we introduce an automatic method for eliminate the effect of AISI 316L iron materials in the implant-bone system based on the application of Compensatory Fuzzy Mathematical Morphology for future investigate about the structural and mechanical properties of bone and cancellous materials.","palabras_clave":"METALLIC IMPLANT|MICRO CT IMAGE|MATHEMATICAL MORPHOLOGY|3D RECONSTRUCTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5394,"idioma_id":4,"titulo":"Classical nThermodynamics from quasi-probabilities","resumen":"The basic idea of a microscopic understanding of thermodynamics is to derive its main features from a microscopic probability distribution. In such a vein, we investigate the thermal statistics of quasi-probabilities?s semiclassical analogs in phase space for the important case of quadratic Hamiltonians, focusing attention in the three more important instances, i.e. those of Wigner, P- and Husimi distributions. Introduction of aneffective temperature permits one to obtain a unified thermodynamic description that encompasses and unifies the three different quasi-probability distributions. This unified description turns out to be classical.","palabras_clave":"SEMICLASSICAL|PHYSICS|P-FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5395,"idioma_id":4,"titulo":"q-Gamow states as continuous linear functionals on analytical test functions","resumen":"We define here q-Gamow states corresponding to Tsallis? q-statistics. We compute for them their norm, mean energy value andthe q-analogue of the Breit?Wigner distribution (a q-Breit?Wigner).","palabras_clave":"GAMOW|STATES|q-GAMOW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5396,"idioma_id":4,"titulo":"Statistical complexity, virial expansion, and van der Waals equation","resumen":"We investigate the notion of LMC statistical complexity with regards to a real gas and in terms of the second virial coefficient. The ensuing results are applied to the van der Waals equation. Interestingly enough, one finds a complexity-interpretation for the associated phase transition.","palabras_clave":"STATISTICAL|COMPLEXITY|REAL|GAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5397,"idioma_id":4,"titulo":"Physical peculiarities of divergences emerging in q-deformed statistics","resumen":"It was found in [A. Plastino, M.C. Rocca, Europhys. Lett. 104, 60003 (2013)] that classical Tsallis theory exhibits poles in the partition function Z and the mean energy U. These occur at a countably set of the q-line. We give here, via a simple procedure, a mathematical account of them. Further,by focusing attention upon the pole-physics, we encounter interesting effects. In particular, for the specific heat, we uncover hidden gravitational effects.","palabras_clave":"PHYSICAL|PECULIARITIES|q-DEFORMED"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5398,"idioma_id":4,"titulo":"Hypergeometric foundations of Fokker-Planck like equations","resumen":"We discover a deep connection between the Fokker?Planckequation and the hypergeometricdifferential equation. The same applies to a non linear generalization of such equation.","palabras_clave":"NONLINEAR|FOKKER|PLANCK|EQUATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5399,"idioma_id":4,"titulo":"Ion transfer of weak acids across liquid | liquid interfaces","resumen":"The main purpose of this paper is to develop a model for the ion transfer ofweak acids across liquid|liquid interfacesincluding ion pairing and non-ideal electrolyte solutions to calculate the half-wave potential. The mainequation derived in this model allows the simulation of different chemical systems, comprising multiple acidbaseequilibria, hydrophilic and hydrophobic neutral weak acids, and different anions, including ion-pair formationin the organic phase. Hence, several representative chemical systems are analysed in detail in order to demonstratethe usefulness of the model. In addition, simple criteria to evaluate the presence of ion-pairs in theorganic phase as well as the calculation of the apparent ion-pair formation constants are included","palabras_clave":"Ion transfer|Weak acid|Liquid|liquid interface|Half-wave potential|Ion pairing|Non ideal electrolyte solutions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5400,"idioma_id":4,"titulo":"Land use impact on chemical and spectroscopical characteristics of soil organic matter in an arid ecosystem","resumen":"Soil organic matter (SOM) storage and composition in ecosystems may undergo change as a result of long-term livestock and soil cultivation, particularly in arid environments. In this work, we evaluated the alterations produced in both the quantity and quality of SOM due to productive management systems. The impact of land use change on SOM, dissolved and hot water-extractable carbon (DOC and HWC), humic substances (HS), humic acids (HA), fulvic acids (FA) and the infrared and visible spectroscopy of HS were studied at three productive sites: total and selective clearings with livestock (TC livestock and SC livestock), total clearing with irrigated agriculture (TC agriculture), and an undisturbed site located in central-western Argentina. The SOM content was higher at the undisturbed and TC agriculture sites. DOC varied among the study sites only during the dry season, while HWC decreased during the wet season, clearly indicating the lability of this fraction. The concentrations of HS, HA, and FA were reduced (50?75 %) by land use change, with the HS composition determined by infrared spectroscopy reflecting a high quantity of polysaccharides in TC agriculture, while the E 4\/E 6 ratio (UV?vis) presented low values at the undisturbed site, indicating a high degree of condensation of aromatic substances. In conclusion, (a) the conversion of native woodlands to livestock systems favored soil C losses, (b) the highest SOM storage recorded in TC agriculture may reflect a greater residue accumulation at the soil surface and (c) the combination of different techniques provided a very good insight into the status of soil degradation.","palabras_clave":"Land Use|Soil Organic Matter|Spectroscopical characteristics|arid ecosystem Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5401,"idioma_id":4,"titulo":"Cytogenetic characterization and B chromosome diversity in direct-developing frogs of the genus Oreobates (Brachycephaloidea, Craugastoridae)","resumen":"Oreobates Jiménez de la Espada, 1872 is a large group of South American frogs with terrestrial reproduction and direct development, located in the superfamily Brachycephaloidea. About 260 brachycephaloidean species have been cytogenetically studied so far, at least with standard techniques. However, this information represents fewer than 17% species of the family Craugastoridae Hedges, Duellman &amp; Heinicke, 2008, where the genus Oreobates is included. In the present work, using a diversity of standard and molecular techniques, we describe the karyotype of O. barituensis Vaira &amp; Ferrari, 2008, O. berdemenos Pereyra, Cardozo, Baldo &amp; Baldo, 2014 and O. discoidalis (Peracca, 1895), from northwestern Argentina. The three species analyzed showed a diploid karyotype with 2n = 22 biarmed chromosomes, fundamental number (FN) = 44, nucleolus organizer regions (NORs) located pericentromerically on pair 7, and a centromeric and pericentromeric C-banding pattern. We observed variations in the chromosomenumber in O. barituensis due the presence of two morphs of B chromosomes, one mediumsizedtelocentric (BT) and another subtelocentric and smaller (Bst). Both B chromosomes are mitotically stable and were recorded in all somatic and germinal cells analyzed. The BT chromosome occurred at a maximum of one per individual (2n = 22+BT), and the other one was observed single (2n = 22 + Bst) or as a pair in two doses (2n = 22 + 2BT). We additionally observed other supernumerary chromosomes in the three species analyzed, all of them euchromatic, small, dot-shaped and with instability during mitoses, showing a frequency of occurrence below 50% in studied specimens. The occurrence of polymorphic and spontaneous chromosomal rearrangements and supernumerary chromosomes is a recurrent feature reported in frogs with terrestrial habits (Brachycephaloidea and Hemiphractidae Peters, 1862), which suggests that Brachycephaloidea may be a promising group for studying the origin and maintenance of B chromosomes in anurans.","palabras_clave":"CYTOGENETICS|ACCESSORY ELEMENTS|RIBOSOMAL DNA|ANURA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5402,"idioma_id":4,"titulo":"Diurnal? Calling activity patterns reveal nocturnal habits in the aposematic toad, Melanophryniscus rubriventris","resumen":"In anuran species with short breeding seasons, an extension of diel activity to the night hours could be favoured to maximize mating opportunities but individuals must deal with physiological and behavioural constraints. We tested this hypothesis in the Yungas red-belly toad, Melanophryniscus rubriventris (Vellard, 1947). We registered diel pattern of male calling activity in two localities using automated recording systems, and related it to abiotic factors as temperature, relative air humidity and precipitation. The diel pattern of vocalization was mainly diurnal. Interestingly, though, nocturnal calling activity was a common feature, representing between 40% and 43% of call records in both localities. Vocal activity was significantly influenced by time of the day and presence of rainfall. Calling males showed high plasticity, with activity in the entire environmental range of relative air humidity and temperature. Nocturnal calling seems to play an important role in the mating strategy of males, and it is probably more frequent in the genus Melanophryniscus than currently assumed. We discuss the implications of our findings in relation to different aspects of ecology of the species and suggest that bright coloration in M. rubriventris might result from a compromise between several non-conflicting functions (e.g. aposematism and thermoregulation).","palabras_clave":"MELANOPHRYNISCUS RUBRIVENTRIS|YUNGAS RED-BELLY TOAD|BREEDING BEHAVIOUR|ADVERTISEMENT CALL|DIEL PATTERN|NOCTURNALITY|APOSEMATISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5403,"idioma_id":4,"titulo":"Caviomorph rodents.main features of their evolution","resumen":"The rich fossil record of caviomorph rodents provides good basis fod understanding the mayor trends of their evolution&nbsp; in southern of South America. The most important features of their evolutionary history are here analyzed since their entrance during the middle Eocene by rafting from Africa. The initial radiation of caviomorphs was complex,with the differentiation of taxa that cannot be assignedto any of the mayor clades (Octodoitoidea, erethizontoidea, Cavioidea, and Chinchilloidea). The evolutionary history occurredthrough pulses of radiation, being the most important those of the middle? Eocene-early Oligocene, late Oligocene and middle to late Miocene.","palabras_clave":"Caviomorpha |Rodentia|Morfología|Evolucion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5404,"idioma_id":4,"titulo":"Quantitative study of FORC diagrams in thermally corrected Stoner Wohlfarth nanoparticles systems","resumen":"The use of FORC diagrams is becoming increasingly popular among researchers devoted tomagnetism and magnetic materials. However, a thorough interpretation of this kind ofdiagrams, in order to achieve quantitative information, requires an appropriate model of thestudied system. For that reason most of the FORC studies are used for a qualitativeanalysis. In magnetic systems thermal fluctuations 'blur' the signatures of the anisotropy,volume and particle interactions distributions, therefore thermal effects in nanoparticlessystems conspire against a proper interpretation and analysis of these diagrams. Motivatedby this fact, we have quantitatively studied the degree of accuracy of the informationextracted from FORC diagrams for the special case of single-domain thermal correctedStoner-Wolhfarth (easy axes along the external field orientation) nanoparticles systems. Inthis work, the starting point is an analytical model that describes the behavior of a magneticnanoparticles system as a function of field, anisotropy, temperature and measurement time.In order to study the quantitative degree of accuracy of our model, we built FORC diagramsfor different archetypical cases of magnetic nanoparticles. Our results show that from thequantitative information obtained from the diagrams, under the hypotheses of the proposedmodel, is possible to recover the features of the original system with accuracy above 95%.This accuracy is improved at low temperatures and also it is possible to access to theanisotropy distribution directly from the FORC coercive field profile. Indeed, oursimulations predict that the volume distribution plays a secondary role being the meanvalue and its deviation the only important parameters. Therefore it is possible to obtain anaccurate result for the inversion and interaction fields despite the features of the volumedistribution.","palabras_clave":"FORC|NANOPARTICLES|ANISOTROPY DISTRIBUTION|FORC PROFILE DISTRIBURTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5405,"idioma_id":4,"titulo":"Adsorption of peroxidase from Raphanus sativus L onto alginate guar gum matrix: kinetic, equilibrium and thermodynamic analysis.","resumen":"This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum - alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature were studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first order rate kinetics and the pseudo-second order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.","palabras_clave":"ALGINATE|GUAR GUM|POLYELECTROLYTE|PEROXIDASE|ADSORPTION|ISOTHERMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5406,"idioma_id":4,"titulo":"The rheological properties of beta amyloid Langmuir monolayers: Comparative studies with melittin peptide","resumen":"We determined the rheological propertiesof b-amyloid Langmuir films at the air\/waterinterface, a peptide whose interfacial structure is extended b-sheet, and compared them with those offilms composed of Melittin (Mel), which adopts an a-helicalconformation at neutral pH. To determine the dilatational and shear moduli weevaluated the response of pure peptide monolayers to an oscillatory anisotropiccompressive work. Additionally, a micro-rheological characterization wasperformed by tracking the diffusion of micrometer sized latex beads onto theinterface. This technique allowed us the detection of different rheologicalbehaviour between monolayers presenting a low shear response. Monolayers of theb-sheet structure-adopting peptides, suchas b-amyloid peptides, exhibited a markedshear (elastic) modulus even at low surface pressures. In contrast, Melmonolayers exhibited negligible shear modulus and the micro-rheological shearresponse was markedly lower than that observed for either Ab1-40 or Ab1-42 amyloid peptides.When Mel monolayers where formed at the interface of an aqueous solution at pH11, we observed an increase in both the lateral stability and film viscosity asdetected by a slower diffusion of the latex beads, in keeping with an increasein b-sheet structure at this high pH (verifiedby ATR and FT-IR measurements). We suggest that the interactions responsiblefor the marked response upon shear observed for b-amyloidpeptide monolayers are the hydrogen bonds of the b-sheetstructure that can form an infinite planar network at the interface.Conversely, a-helical Mel peptide lack of theseinter-molecular interactions and, therefore the shear contribution was negligible.We propose that the secondary structure isimportant for modulating the rheological behavior ofshort peptide monolayers regardless of the mass density or surfacecharge at the surface. ","palabras_clave":"ELASTIC SHEAR|MEMBRANE DISRUPTING PEPTIDES|SECONDARY STRUCTURE|RHEOLOGICAL BEHAVIOR|PEPTIDE LANGMUIR MONOLAYERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5407,"idioma_id":4,"titulo":"Extracellular histones reduce survival and angiogenic responses of late outgrowth progenitor and mature endothelial cells","resumen":"ESSENTIALS: Extracellular histones are highly augmented in sites of neovessel formation, such as regeneration tissues. We studied histone effect on survival and angiogenic activity of mature and progenitor endothelial cells. Extracellular histones trigger apoptosis and pyroptosis and reduce angiogenesis in vivo and in vitro. Histone blockade can be useful as a therapeutic strategy to improve angiogenesis and tissue regeneration.BACKGROUND:Extracellular histones are highly augmented in sites of neovessel formation, like regeneration tissues. Their cytotoxic effect has been studied in endothelial cells, although the mechanism involved and their action on endothelial colony-forming cells (ECFCs) remain unknown.OBJECTIVE:To study the effect of histones on ECFC survival and angiogenic functions and compare it with mature endothelial cells.METHODS AND RESULTS:Nuclear morphology analysis showed that each human recombinant histone triggered both apoptotic-like and necrotic-like cell deaths in both mature and progenitor endothelial cells. While H1 and H2A exerted a weak toxicity, H2B, H3 and H4 were the most powerful. The percentage of apoptosis correlated with the percentage of ECFCs exhibiting caspase-3 activation and was zeroed by the pan-caspase inhibitor Z-VAD-FMK. Necrotic-like cell death was also suppressed by this compound and the caspase-1 inhibitor Ac-YVAD-CMK, indicating that histones triggered ECFC pyroptosis. All histones, at non-cytotoxic concentrations, reduced migration and H2B, H3 and H4 induced cell cycle arrest and impaired tubulogenesis via p38 activation. Neutrophil-derived histones exerted similar effects. In vivo blood vessel formation in the quail chorioallantoic membrane was also reduced by H2B, H3 and H4. Their cytotoxic and antiangiogenic effects were suppressed by unfractioned and low-molecular-weight heparins and the combination of TLR2 and TLR4 blocking antibodies.CONCLUSIONS:Histones trigger both apoptosis and pyroptosis of ECFCs and inhibit their angiogenic functions. Their cytotoxic and antiangiogenic effects are similar in mature endothelial cells and disappear after heparin addition or TLR2\/TLR4 blockade, suggesting both as therapeutic strategies to improve tissue regeneration.","palabras_clave":"angiogenesis|histones|endothelial cells|p38"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5408,"idioma_id":5,"titulo":"Zooarqueología y tafonomía en pastores actuales  del sur de Mendoza","resumen":"Los puesteros del centro oeste de la Argentina son pastores que viven durante el invierno en las planicies y en verano practican la trashumancia hacia sectores altos de la cordillera de los Andes. En este trabajo se presenta un acercamiento al consumo de fauna por parte de los puesteros, desde una perspectiva zooarqueológica y tafonómica, según la cual se analiza si existe variabilidad entre los distintos asentamientos en cuanto al con-sumo de fauna silvestre, fauna doméstica criada en los puestos o fauna obtenida en el mercado. Se postula que habría una relación entre el consumo de los distintos tipos de fauna, el grado de movilidad residencial y el grado de contacto con las economías urbanas. Para una mejor comprensión de los procesos involucrados, se muestrearon puestos con distinto grado de sedentarismo. En este trabajo presentamos los resultados de las primeras muestras recolectadas. Estos análisis son acompañados por un enfoque tafonómico, de acuerdo con el cual evaluamos si existen procesos posdepositacionales diferenciales en cada uno de los conjuntos analizados que estuvieran condicionando los resultados obtenidos.","palabras_clave":"Puesteros|Trashumancia|Fauna silvestre|Tafonomía"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5409,"idioma_id":4,"titulo":"The phytoestrogen genistein enhances multidrug resistance in breast cancer cell lines by translational regulation of ABC transporters","resumen":"Breast cancer is the most frequent malignancy in women. Multidrug resistance due to overexpression of ABC drug transporters is a common cause of chemotherapy failure and disease recurrence. Genistein (GNT) is a phytoestrogen present in soybeans and hormone supplements. We investigated the effect of GNT on the expression and function of ABC transporters in MCF-7 and MDA-MB-231 breast cancer cell lines. Results demonstrated an induction at the protein level of ABCC1 and ABCG2 and of ABCC1 in MCF-7 and MDA-MB-231, respectively. MCF-7 cells showed a concomitant increase in doxorubicin and mitoxantrone efflux and resistance, dependent on ABCG2 activity. ABCC1 induction by GNT in MDA-MB-231 cells modified neither drug efflux nor chemoresistance due to simultaneous acute inhibition of the transporter activity by GNT. All inductions took place at the translational level, as no increment in mRNA was observed and protein increase was prevented by cycloheximide. miR-181a, already demonstrated to inhibit ABCG2 translation, was down-regulated by GNT, explaining translational induction. Effects were independent of classical estrogen receptors. Results suggest potential nutrient-drug interactions that could threaten chemotherapy efficacy, especially in ABCG2-expressing tumors treated with substrates of this transporter.","palabras_clave":"GENISTEIN|BREAST CANCER CELLS|MULTIDRUG RESISTANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5410,"idioma_id":4,"titulo":"Modulation of Expression and Activity of ABC Transporters by the Phytoestrogen Genistein. Impact on Drug Disposition","resumen":"ATP binding cassette (ABC) transporters are involved in drug absorption, distribution and elimination. They also mediate multidrug resistance in cancer cells. Isoflavones,such as genistein (GNT), belong to a class of naturally-occurring compounds found at high concentrations in commonly consumed soya based-foods and dietary supplements. GNT and its metabolites interact with ABC transporters as substrates, inhibitors and\/or modulators of their expression.This review compiles information about regulation of ABC transporters by GNT with special emphasis on the three major groups of ABC transporters involved in excretion of endo- and xenobiotics as follows: Pglycoprotein (MDR1, ABCB1), a group of multidrug resistance associated proteins (MRPs, ABCC subfamily) and ABCG2 (BCRP), an ABC half-transporter. The impact of these regulations on potential GNT-drug interactions is further considered.","palabras_clave":"ATP BINDING CASSETTE TRANSPORTERS|BREAST CANCER RESISTANCE PROTEIN|GENISTEIN|NUTRIENT-DRUG INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5411,"idioma_id":4,"titulo":"Enhanced gauge symmetry and winding modes in Double Field  Theory","resumen":"We provide an explicit example of how the string winding modes can be incorporatedin double &#64257;eld theory. Our guiding case is the closed bosonic string compacti&#64257;ed on acircle of radius close to the self-dual point, where some modes with non-zero winding or discrete momentum number become massless and enhance the U(1) × U(1) symmetry to SU(2) × SU(2). We compute three-point string scattering amplitudes of massless and slightly massive states, and extract the corresponding e&#64256;ective low energy gauge&#64257;eld theory. The enhanced gauge symmetry at the self-dual point and the Higgs-likemechanism arising when changing the compacti&#64257;cation radius are examined in detail. Theextra massless &#64257;elds associated to the enhancement are incorporated into a generalized frame with O(d+3,d+3) O(d+3)×O(d+3) structure, where d is the number of non-compact dimensions. We devise a consistent double &#64257;eld theory action that reproduces the low energy stringe&#64256;ective action with enhanced gauge symmetry. The construction requires a truly nongeometric frame which explicitly depends on both the compact coordinate along the circleand its dual.","palabras_clave":"STRING THEORY|DUALITY|COMPACTIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5412,"idioma_id":4,"titulo":"Innovative Immobilization Matrices","resumen":"We present a brief survey of some of the recent work of Professor Luis E. Diaz, performed together with his students and collaborators at the University of Buenos Aires. Dr Luis E. Diaz has been involved in research on biochemical and pharmaceutical sciences solving scientific and industry problems for over 40 years until he passed away. Prof. Diaz scientific interests included various topics from NMR spectroscopy to biomedicine but fundamentally he focused in various aspects of chemistry (analytical, organic, inorganic and environmental). This is not a complete survey but a sampling of prominent projects with a focus on some of his recent publications","palabras_clave":"sol-gel|matrices|silica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5413,"idioma_id":4,"titulo":"Screening and characterization of molecules that modulate the biological activity of IFNs-I","resumen":"Type I Interferons (IFNs-I) are species-specific glycoproteins which play an important role as primary defence against viral infections and that can also modulate the adaptive immune system. In some autoimmune diseases, interferons (IFNs) are over-produced. IFNs are widely used as biopharmaceuticals for a variety of cancer indications, chronic viral diseases, and for their immuno-modulatory action in patients with multiple sclerosis; therefore, increasing their therapeutic efficiency and decreasing their side effects is of high clinical value. In this sense, it is interesting to find molecules that can modulate the activity of IFNs. In order to achieve that, it was necessary to establish a simple, fast and robust assay to analyze numerous compounds simultaneously. We developed four reporter gene assays (RGAs) to identify IFN activity modulator compounds by using WISH Mx2\/EGFP, HeLa-Mx2\/EGFP, A549-Mx2\/EGFP, and HEp2 Mx2\/EGFP reporter cell lines (RCLs). All of them present a Z? factor higher than 0.7. By using these RGAs, natural and synthetic compounds were analyzed simultaneously. A total of 442 compounds were studied by the Low Throughput Screening (LTS) assay using the four RCLs to discriminate between their inhibitory or enhancing effects on IFN activity. Some of them were characterized and 15 leads were identified. Finally, one promising candidate with enhancing effect on IFN-&#945; activity and five compounds with inhibitory effect were described.","palabras_clave":"Cell-based RGA|hIFN-I|Natural and synthetic compounds|Low Throughput Screening|Mx2\/EGFP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5414,"idioma_id":5,"titulo":"Emociones, toma de decisiones y consumo de alcohol en jóvenes universitarios","resumen":"Según  la  hipótesis  del  marcador  somático,  la  toma  de  decisiones  resulta  adaptativa  cuandolos  procesos  emocionales  se  integran  a  la  actividad  cognitiva.  La  primacía  del  sistema  afectivo  sobre  el  control  cognitivo  en  los  jóvenes  se  relaciona  con  una  toma  de  decisiones  enfunción  de  la  gratificación  inmediata,  sin  tener  en  cuenta  posibles  consecuencias  perjudiciales  futuras.  El  consumo  intensivo  de  alcohol,  muy  frecuente  en  los  jóvenes,  se  asociaa  una  acrecentada  toma  de  decisiones  desadaptativa.  El  objetivo  del  presente  estudio  fueevaluar  la  toma  de  decisiones  en  jóvenes  de  ambos  sexos  consumidores  moderados  e  intensivos  de  alcohol,  según  el  tipo  de  inducción  emocional  a  la  que  fueron  expuestos.  Medianteun  dise? no  factorial  con  pre-  y  postest,  se  estudió  la  toma  de  decisiones  en  79  participantes  de  18  a  29  a? nos,  antes  y  después  de  la  inducción  emocional  positiva  o  negativa.  Paraevaluar  la  toma  de  decisiones,  se  administraron  las  pruebas  Iowa  Gambling  Task  y  CognitiveBias  Task.  Para  inducir  emociones  se  utilizaron  fragmentos  de  películas.  Además,  se  registró  la  frecuencia  cardíaca  durante  la  experimentación.  Como  resultado,  se  encontró  que  lasmujeres  inducidas  negativamente  y  los  hombres  inducidos  positivamente  obtuvieron  peorrendimiento  en  la  Iowa  Gambling  Task.  La  frecuencia  cardíaca  disminuyó  durante  las  sesiones  experimentales  respecto  del  periodo  basal.  Los  consumidores  intensivos  tuvieron  peorrendimiento  en  la  Cognitive  Bias  Task  respecto  de  los  consumidores  moderados.  Se  concluyeque  la  experiencia  emocional  y  el  tipo  de  consumo  de  alcohol  influyen  sobre  la  toma  dedecisiones  en  los  jóvenes  universitarios.","palabras_clave":"Emociones|Toma de Decisiones|Consumo de Alcohol|Juventud"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5415,"idioma_id":5,"titulo":"Diversity of cingulate xenarthrans in the middle-late Eocene of Northwestern Argentina","resumen":"The study of Paleogene mammals of intermediate and low latitudes has increased in the last decades and has been clearly demonstrated their importance in the comprehension of the evolution and faunistic changes outside Patagonia. The study of these faunas permits establishing new comparisons among contemporaneous faunistic associations, completing the distributional patterns, and evaluating evolutionary changes in the lineages in relation to the climatic conditions prevailing in each of the different regions. In this work we study the diversity of Dasypodidae recovered from the Geste Formation (Northwestern Argentina). Bearing levels of Geste Formation were referred alternatively to a Barrancan subage of Casamayoran SALMA (middle Eocene, Lutetian?Bartonian) or a Mustersan SALMA (middle?late Eocene, Bartonian?Priabonian) on faunistic comparations with their equivalent in Patagonia. Although, absolute isotopic data indicates ca. 37?35 Ma (late Eocene, Priabonian). We described the following taxa of Dasypodidae: (i) Dasypodinae Astegotheriini: cf. Astegotherium sp., Prostegotherium sp. nov.?, Parastegosimpsonia cf. P. peruana; (ii) Dasypodinae indet.; (iii) Euphractinae Euphractini: Parutaetus punaensis sp. nov.; (iv) Dasypodidae incertae sedis: Pucatherium parvum, Punatherium catamarcensis gen. et sp. nov. In comparison with other beds bearing Eocene cingulate faunas from Northwestern Argentina, Geste Formation presents the greatest diversity of dasypodids. This association is consistent with a late Eocene age and shows a taxonomic and biogeographic relevant features given by a unique specific composition: (i) It differs from that known for contemporaneous faunas form Southern latitudes and younger associations from more tropical areas; (ii) it includes genera with close affinities to those distant areas; (iii) it presents unique taxa typical from Eocene units exposed at Northwestern Argentina. This highlight the evolutionary and biogeographic meaning of the cingulate of the Geste Formation and supports the idea that the faunistic regionalization probably obeyed to latitudinal than to temporal factors.","palabras_clave":"Mammalia|Cingulata|Dasypodidae| armadillo|low latitudes|Palaeogene|Central Andean puna|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5416,"idioma_id":4,"titulo":"Cold Stress Tolerance during Early Growth Stages of Naturalized Helianthus petiolaris Populations","resumen":"In the last 10 years the sunflower crop area of Argentina has shifted towards the Southwest as a result of the expansion of soybean cultivation. Early sowings require greater tolerance to low temperatures in order to optimize the use of water resources by the crop, especially in the seedling stage, when cold stress limits growth and productivity. Helianthus petiolaris is a wild genetic resource that has contributed to the production of sunflower cultivars. The goal of this study was to evaluate the tolerance to low temperatures in H. petiolaris plants. Morphological traits (plant height, leaf width and leaf length) and physiological traits (electrolyte leakage, chlorophyll content, and glucose content) were compared in eight accessions having an early germination in the field and one with normal life cycle. Acclimated and non-acclimated plants were subjected to cold stress followed by a recovery period. Physiological traits during early growth stages showed differences in cold tolerance and for acclimation ability between accessions, suggesting the existence of heritable components. Four accessions were the most cold-tolerant among the studied populations, suggesting a natural adaptation related to the geographical site. Results point out that H. petiolaris is a useful genetic resource to contribute to sunflower breeding in the search of cold tolerant cultivars.","palabras_clave":"morphological traits|glucose content|electrolyte leakage| chlorophyll content"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5417,"idioma_id":4,"titulo":"On the properties of compact groups identified in different photometric bands","resumen":"Historically, compact group catalogues vary not only in their identification algorithms and selection functions, but also in their photometric bands. Differences between compact group catalogues have been reported. However, it is difficult to assess the impact of the photometric band in these differences given the variety of identification algorithms. We used the mock lightcone built by Henriques et al. (2012) to identify and compare compact groups in three different photometric bands: K, r, and u. We applied the same selection functions in the three bands, and found that compact groups in the u-band look the smallest in projection, the difference between the two brightest galaxies is the largest in the K-band, while compact groups in the r-band present the lowest compactness. We also investigated the differences between samples when galaxies are selected only in one particular band (pure compact groups) and those that exist regardless the band in which galaxies were observed (common compact groups). We found that the differences between the total samples are magnified, but also some others arise: pure-r compact groups are the largest in projection; pure-u compact groups have the brightest first ranked galaxies, and the most similar two first ranked galaxies; pure-K compact groups have the highest compactness and the most different two first ranked galaxies; and common compact groups show the largest percentage of physically dense groups. Therefore, without a careful selection and identification of the samples, the characteristic features of group properties in a particular photometric band could be overshadowed.","palabras_clave":"GALAXIES:CLUSTERS:GENERAL|METHODS:STATISTICAL|METHODS:DATA ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5418,"idioma_id":4,"titulo":"Sarcocystosis in wild red deer (Cervus elaphus) in Patagonia, Argentina","resumen":"Sarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62% (38\/62) of heart, and in 22% (3\/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (&#8804; 1 µm), with scarce and separated ribbon-like protrusions. A total of 3 partial and 1 full 18S rRNA gene sequences were obtained, and showed the highest identity (&#8805; 99%) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.","palabras_clave":"SARCOCYSTIS TAENIATA|RED DEER|ARGENTINEAN NONNATIVE UNGULATE|TEM|SEQUENCING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5419,"idioma_id":4,"titulo":"TP53 codon 72 polymorphism predicts chronic myeloid leukemia susceptibility and treatment outcome","resumen":"BCR-ABL1 gene is a key molecular marker of chronic myeloid leukemia (CML), but it is still unclear which molecular factors may influence CML risk or lead to variable responses to tyrosine kinase inhibitors (TKIs). The aim of this study was to investigate the impact of TP53 c.213 G N C(Arg72Pro; rs1042522) polymorphism on CML risk and its correlation with clinical outcome. Peripheral blood samples from 141 treated CML patients and 141 sex- and age-matched healthy individuals were genotyped by PCR-RFLP. Standard genetic models for disease penetrance were evaluated by logistic regression analysis and Kaplan-Meier method was performed to estimate survival curves. Our study suggests that TP53 c.213 G N C polymorphism may be involved in CML development considering a recessive model (p= 0.01; OR: 0.19; CI: 0.06?0.68). In addition, a non homogenous distribution was found for this polymorphism in males and patients youngers than 50 years (p=0.02). According to clinical response, TP53-GG genotype was associated with higher levels of BCR-ABL1 transcripts (p = 0.04) and shorter event free survival (p=0.04). Moreover, a trend toward significance was found for failure free survival (p=0.06) and time to imatinib failure (p=0.08). In conclusion, our data suggest that a; TP53 c.213 G NCmaybe a potential biomarker of CML susceptibility and clinical outcome.","palabras_clave":"LMC|Polimorfismo|PT53"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5420,"idioma_id":5,"titulo":"VERIFICACIÓN DEL PH DE DIFERENTES SOLUCIONES DE HIPOCLORITO DE SODIO","resumen":"La solución de hipoclorito de sodio, en sus diferentes concentraciones, constituye la primera elección mundial como sustancia irrigadora de los conductos radiculares.Es por esto la importancia de mantener el pH de la solución para obtener una buena desinfección, y con eso aumentar las posibilidades de alcanzar el éxito endodóntico.El objetivo de este estudio es verificar el pH en diferentes marcas comerciales de hipoclorito de sodio.Se seleccionaron diversas marcas comerciales de diferentes concentraciones de hipoclorito de sodio encontradas a la venta. Todas las soluciones analizadas se encontraban en envases cerradosPara el análisis del potencial hidrogénico (pH) se utilizó un medidor potenciométrico de pH. De acuerdo al análisis de los datos obtenidos, las soluciones de hipoclorito de sodio presentarABSTRACTThe solution of sodium hypochlorite at different concentrations, is the first global election as substance irrigating root canals.That is why the importance of maintaining the pH of the solution to obtain a good disinfection, and thereby increase the chances of reaching the endodontic success.Therefore the aim of this study is to check the pH in different brands of sodium hypochlorite.Materials and Methods: For this study several shopping and different concentrations of sodium hypochlorite found for sale brands were selected. All solutions were analyzed in closed containersHidrogénico for analyzing potential (pH) a potentiometric pH meter was used. According to the analysis of the data obtained, the sodium hypochlorite solutions showed an alkaline pH. Greater control of quality of sodium hypochlorite solutions found for sale and used as irrigating during endodontic therapy is suggested.on un pH alcalino.","palabras_clave":"HIPOCLORITO DE SODIO|PH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5421,"idioma_id":4,"titulo":"Effects of chlorpyrifos on enzymatic systems of Cydia pomonella (Lepidoptera: Tortricidae) adults","resumen":"The control program of codling moth (Cydia pomonella L.) in the R´&#305;o Negroand Neuqu´en Valley is intended to neonate larvae. However, adults may be subjected to sublethal pesticide concentrations generating stress which might enhance both mutation rates and activity of the detoxification system. This study assessed the exposure effects of chlorpyrifos on target enzyme and, both detoxifying and antioxidant systems of surviving adults from both a laboratory susceptible strain (LSS) and a field population (FP). The results showed that the FP was as susceptible to chlorpyrifos as the LSS and, both exhibiteda similar chlorpyrifos-inhibitory concentration 50 (IC50) of acetylcholinesterase (AChE).The FP displayed higher carboxylesterase (CarE) and 7-ethoxycoumarine O-deethylase (ECOD) activities than LSS. Both LSS and FP showed an increase on CarE activity after the exposure to low-chlorpyrifos concentrations, followed by enzyme inhibition at higher concentrations. There were no significant differences neither in the activities of glutathione S-transferases (GST), catalase (CAT) and superoxide dismutase (SOD) nor in the reduced glutathione (GSH) content between LSS and FP. Moreover, these enzymes were unaffected by chlorpyrifos. In conclusion, control adults from the FP exhibited higherCarE and ECOD activities than control adults from the LSS. AChE and CarE activities were the most affected by chlorpyrifos. Control strategies used for C. pomonella, such as rotations of insecticides with different modes of action, will probably delay the evolution of insecticide resistance in FPs from the study area.","palabras_clave":"acetylcholinesterase|antioxidant system|chlorpyrifos|codling moth"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5422,"idioma_id":4,"titulo":"Thermodynamic conditions during growth determine the magnetic anisotropy in epitaxial thin-films of La0.7Sr0.3MnO3","resumen":"The suitability of a particular material for use in magnetic devices is determined by the process of magnetization reversal\/relaxation, which in turn depends on the magnetic anisotropy. Therefore, designing new ways to control magnetic anisotropy in technologically important materials is highly desirable. Here we show that magnetic anisotropy of epitaxial thin-films of half-metallic ferromagnet La0.7Sr0.3MnO3 (LSMO) is determined by the proximity to thermodynamic equilibrium conditions during growth. We performed a seriesof x-ray diffraction and ferromagnetic resonance (FMR) experiments in two different sets of samples: the first corresponds to LSMO thin-films deposited under tensile strain on (0 0 1) SrTiO3 by pulsed laser deposition (PLD; far from thermodynamic equilibrium); the second were deposited by a slow chemical solution deposition (CSD) method, under quasi-equilibrium conditions. Thin films prepared by PLD show fourfold in-plane magnetic anisotropy, with an overimposed uniaxial term. However, the uniaxial anisotropy is completely suppressed in the CSD films. This change is due to a different rotation pattern of MnO6 octahedra to accomodate epitaxial strain, which depends not only on the amplitude of tensile stress imposed by the STO substrate, but also on the growth conditions. Our results demonstrate that the nature and magnitude of the magnetic anisotropy in LSMO can be tuned by the thermodynamic parameters during thin-film deposition.","palabras_clave":"MAGNETIC ANISOTROPY|THIN FILM DEPOSITION|MAGNETIC RESONANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5423,"idioma_id":4,"titulo":"Structures and vibrational spectra of nitro-benzo[a]pyrene and nitro-benzo[e]pyrene isomers: a density functional study","resumen":"Toxicity and reactivity of nitro polycyclics have been correlated with their molecular structure and electronic properties, in particular with the nitro substituent orientation. Experimental data are scarce for mononitro derivatives of benzo[a]pyrene (BaP) and benzo[e]pyrene (BeP), so density functional B3LYP\/6-311++G(d,p) calculations have been performed on all of the isomers of nitro-BaP and nitro-BeP in their singlet and triplet states. The most stable configurations are planar ones in which NO2 interacts with the aromatic &#960;-electron system, although the majority of structures are non-planar. Nitro substitution in the bay region of both BaP and BeP results in two stable singlet structures with very different geometries; the higher energy states show a strong O?C interaction that induces sp3 hybridization in the aromatic carbon atom. The calculated IR spectra display differences between planar and non-planar structures that can be used to predict the nitro group orientation and thereby the potential toxicity of each isomer.","palabras_clave":"Benzo[a]pyrene|nitro-aromatic|Benzo[e]pyrene| toxicity prediction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5424,"idioma_id":5,"titulo":"Vegetación y Flora de afloramientos basálticos del centro de Argentina","resumen":"En este trabajo se relevó, caracterizó la flora y exploró la variación composicional de la vegetación en afloramientos de basaltos del centro de Argentina y se determinaron las asociaciones entre la composición florística y diferentes variables climáticas representativas de la variación a escala regional. Se relevaron cuatro complejos de afloramientos desde los 400 hasta 900 m snm, estimándose abundancia-cobertura de todas las plantas vasculares presentes en 48 censos. Los atributos composicionales estructurales de la vegetación revelaron diferencias significativas entre afloramientos de latitudes diferentes. La composición florística regional está asociada a procesos biogeográficos-climáticos. Se confirma la importancia de estas variables en la estructuración del hábitat y filtrado abiótico de especies y la importancia de su conocimiento para establecer prioridades en su conservación. Los afloramientos basálticos, aún no explotados, constituyen hábitats importantes para la conservación de la biodiversidad, como refugios de especies endémicas y especies sobreutilizadas.","palabras_clave":"afloramientos basálticos|clima|Flora|comunidades vegetales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5426,"idioma_id":4,"titulo":"Critical thickness for stripe domain formation in FePt thin films: dependence on residual stress and film thickness","resumen":"Magnetically soft FePt thin films of varying thickness (20 nmd100 nm) were sputter-depositedat different Ar pressures in order to systematically modify the residual stress and hence the magnetic anisotropy. The magnetic domain structure of FePt thin films showed a transition from planar to nearly parallel stripes above a critical thickness, dcr, which was found to depend on an anisotropy contribution perpendicular to the film plane, originated essentially in magnetoelastic effects. A careful structural characterization was made in order to obtain the strain and the stress induced magnetic anisotropy in the samples. Vibrating sample magnetometry and magnetic force microscopy were used to investigate the changes occurring in the magnetic domain structure and the critical thickness of each set of films. Joining together structural and magnetic results, we have been able to construct a phase diagram that divided regions of different domain structures, either by changing the film thickness or the perpendicular magnetic anisotropy. The experimental resultscould be satisfactorily explained by using a model developed by Murayama. The observed dependence of the magnetic properties of soft FePt thin films on the fabrication conditions opens the possibility to tune the magnetic domain configuration from planar to stripe-like domains by changing the argon sputtering pressure used during film deposition.","palabras_clave":"FePt|Magnetic Thin Films|Stripe Domains|Magnetic Anisotropy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5427,"idioma_id":4,"titulo":"High performance electronic device for the measurement of the inverse spin Hall effect","resumen":"We have developed a high performance analog electronic device that can be used for the measurement of the inverse spin Hall effect (ISHE) as a function of the applied magnetic field. The electronic circuit is based on the synchronous detection technique with a careful selection of the active components in order to optimize the response in this application. The electronic accessory was adapted for the simultaneous measurement of the ISHE signal and the microwave absorption in an electron spin resonance spectrometer and tested with a bilayer sample of 5 nm of permalloy (Ni80Fe20) and 5 nm of tantalum. The response of the electronic device was characterized as a function of the microwave power, the amplitude and frequency of the modulation signal, and the relative phase between signal and reference. This last characterization reveals a simple method to put in phase the signal with the reference. The maximum signal to noise ratio was achieved for a modulation frequency between 6 and 12 kHz, for the largest possible values of field modulation amplitude and microwave power.","palabras_clave":"Inverse Spin Hall Effect|Magnetic Thin Films|Spintronics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5428,"idioma_id":4,"titulo":"Effects of melatonin and gonadal androgens on cell proliferation in the pituitary of viscachas (Lagostomus maximus maximus)","resumen":"Objectives: Melatonin and androgens are involved in the regulation of cell proliferation. However, the effect of these hormones on pituitary pars distalis (PD) of male viscachas is not fully understood. In the present study, we  analyzed the melatonin and gonadal androgens effects on proliferating cell nuclear antigen (PCNA) expression. Materials and methods: Pituitary glands from fetus, immature, prepubertal, and adult viscachas during their reproductive cycle, after melatonin administration and after castration were used. PCNA-ircells were detected by immunocytochemistry and morphometrically quantified by image analysis. Results: the total percentage of PCNA-ir cells varied seasonally in the adult pituitary, with maximum values during the reproductive period and minimum values during the gonadal regression period. The percentage of PCNA-ir cells increased after melatonin administration, whereas it decreased after castration. The caudal end and ventral region were the PD zones which were most affected by seasonal variations and castration. The PCNA expression was highest in fetal pituitary from midpregnancy. The number of PCNA-ir cells decreased during sexual maturity. Conclusions: Our results demonstrated the effect of gonadal androgens on cell proliferation during the reproductive period and sexual maturity of animals. Exogenous melatonin increased PD cell proliferation in adults. Thus, these hormones might be involved in different mechanisms that regulate the cell renewal of PD in this seasonally breedingrodent.","palabras_clave":"LAGOSTOMUS|PITUITARY|PCNA|MELATONIN-ANDROGEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5429,"idioma_id":4,"titulo":"Toxicity assessment of four insecticides with different modes of action on pupae and adults of Eriopis connexa (Coleoptera: Coccinellidae), a relevant predator of the Neotropical Region","resumen":"Pesticides can be toxic to nontarget organisms including the natural enemies of agricultural pests, thus reducing the biodiversity of agroecosystems. The lethal and sublethal effects of four insecticides with different modes of action-pyriproxyfen, teflubenzuron, acetamiprid, and cypermethrin-were evaluated on pupae and adults of Eriopis connexa, an effective predator in horticultural crops. Pupal survival was reduced by pyriproxyfen (26 %) and cypermethrin (41 %). Malformations in adults emerged from treated pupae were observed after acetamiprid (82.7 and 100 % for 100 and 200 mg a.i.\/l, respectively), pyriproxyfen (48.6 %), and cypermethrin (13.3 %) treatments. A longer mean oviposition time was also observed in adults emerged from pupae treated with cypermethrin. Moreover, the latter insecticide as well as teflubenzuron did not reduce reproductive parameters, whereas females emerged from pyriproxyfen-treated pupae were not be able to lay eggs even when females showed large abdomens. Upon exposure of adults, survival was reduced to approximately 90 % by acetamiprid, but no reduction occurred with pyriproxyfen, teflubenzuron, or cypermethrin though the fecundity at fifth oviposition time of the female survivors was reduced. Pyriproxyfen decreased the hatching at all the oviposition times tested, whereas fertility was reduced in the fourth and fifth ovipositions by teflubenzuron and in the first and third ovipositions by cypermethrin. In conclusion, all four insecticides tested exhibited lethal or sublethal effects, or both, on E. connexa. The neurotoxic insecticides were more harmful than the insect-growth regulators, and pupae were more susceptible than adults. The toxicity of insecticides on the conservation of predators in agroecosystems of the Neotropical Region is discussed.","palabras_clave":"Eriopis connexa|Lethal effects|Sublethal effects| Insecticides selectivity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5430,"idioma_id":4,"titulo":"The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas","resumen":"Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia.Objective: To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationshipwith Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined inMVMand BMfrom normal and preeclamptic placentas and in detergent resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance.Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry.Cav-1 was significantly reduced inMVMfrom preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium.Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesiclesmay disrupt the ability of SMto assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.","palabras_clave":"TROPHOBLAST|HUMAN PLACENTA|CAVEOLIN 1|LIPID COMPOSITION|PREECLAMPSIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5431,"idioma_id":4,"titulo":"Compensatory responses in plant-herbivore interactions: Impacts of insects on leaf water relations","resumen":"Herbivore damage to leaveshas been typically evaluated in terms of fractions of area removed; however morpho-physiologicalchanges in the remaining tissues can occur in response to removal. We assessedthe effects of partial removal of the leaf mesophyll by Caliroa cerasi (Hymenoptera) on leaf hydraulic conductance (Kleaf),vascular architecture, water relations and leaf size of three Prunus avium cultivars. The insect feedson the leaf mesophyll leaving the vein network intact (skeletonization). Withineach cultivar there were trees without infestations and trees chronicallyinfested, at least over the last three years. Leaf size of intact leaves tendedto be similar during leaf expansion before herbivore attack occurs across infestedand non-infested trees. However, after herbivore attack and when the leaves were fully expanded, damagedleaves were smaller than leaves from non-infested trees. Damaged area variedbetween 21 to 31% depending on cultivar. The non-disruption of the vascularsystem together with either vein density or capacitance increased in damagedleaves resulted in similar Kleaf and stomatal conductance ininfested and non-infested trees. Non-stomatal water loss from repeated leafdamage resulted in lower leaf water potentials in two of the infestedcultivars. &nbsp;Lower osmotic potentials wereobserved in infested plants of these two cultivars. We suggest that carbon andwater costs associated to partial mesophyll removal, such as the increase in activesolutes and loss of water from damaged areas may be accountable for the smallerleaf size in all cultivars experiencing recurrent infestation. Our results showthat skeletonization resulted in compensatory changes in terms of waterrelations and hydraulics traits and in cultivar-specific physiological changesin phylogenetic related P. avium. Partialremoval of mesophyll leaving the veins intact can be used as an experimental treatmentto assess the effects of modifying the extra-vascular pathways of water movementwithin the leaves. &nbsp;","palabras_clave":"CALIROA CERASI|LEAF HYDRAULIC CONDUCTANCE|LEAF SIZE|OSMOTIC ADJUSTMENT|PRUNUS AVIUM|VASCULAR ARCHITECTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5432,"idioma_id":4,"titulo":"Improving the antidepressant action of sertraline by co-crystallization with the antioxidant agent coumarin 3-carboxylic acid. Structural determinations","resumen":"To improve the antidepressant action of sertraline a new salt with coumarin-3-carboxylate anion (SerHCCA)has been synthesized by two different methods and characterized by FTIR spectroscopy andstructural determinations by X-ray diffraction methods. The new salt is stabilized by strong intermolecularH-bonds involving the protonated amine group of SerH and the deprotonated carboxylate groupof CCA. These findings can be correlated with the interpretation of the infrared spectrum. The salt,sertraline (SerHCl) and the sodium salt of coumarin-3-carboxylate (NaCCA) were orally administeredmale Wistar rats (10 mg\/kg, based on sertraline). Rats were evaluated in separate groups by means of theforced swimming (FST). SerH-CCA produced antidepressant effects in a magnitude that exceeded SerHClindividual effects. None of these treatments affected activity levels by the open field OFT tests. We havealso determined that the ion pair also improve the binding to bovine serum albumin (BSA) of the drugbut retain its antimicrobial activity. It is reasonable to conclude that the replacement of chloride anion bya large organic anion in sertraline strengthens the pharmacological action of the native drug, binding toBSA with higher activity and retaining the antimicrobial activity of the antidepressant compound.","palabras_clave":"Antidepressants|Sertraline-coumarin-3-carboxylate|Structural determination|Wistar rat tests|BSA binding"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5433,"idioma_id":4,"titulo":"BMP2,  4,  6  and  BMPR1B  are  altered  from  early  stages  of  bovine  cystic  ovarian  disease  development","resumen":"Cystic ovarian disease (COD) is an important cause ofsubfertility in dairy cattle. Bone Morphogenetic Proteins (BMPs), mainly BMP2,BMP4, and BMP6, play a key role in female fertility. In this study, we hypothesizedthat an altered BMP system is associated with ovarian alterations contributingto COD pathogenesis. Therefore, we examined the expression of BMP2, BMP4, BMP6and BMP receptor 1B (BMPR1B) in the ovaries of animals with spontaneous orACTH-induced COD, as well as during the development of the disease, in a modelof follicular persistence induced by low doses of progesterone (at 5, 10 and 15days of follicular persistence). Results showed changes in BMP2, BMP4 and BMP6 expressionduring folliculogenesis, in granulosa and theca cells in the COD groups, aswell as at the different stages of follicular persistence. Results also showedchanges in BMPR1B expression in developing follicles in animals with COD, and atthe initial stages of follicular persistence (P5). Comparison between groupsshowed significant differences, mainly in BMP4 and BMP6 expression, ingranulosa and theca cells of different follicular categories. The expression ofthese BMPs was also increased in cystic and persistent follicles, in relationto antral follicles of the control group. BMPR1B showed high expression incystic follicles. Together, these results may indicate an alteration in BMPs,especially in BMP4 and BMP6, as well as in BMPR1B, which occurs early infolliculogenesis and incipiently during the development of COD, and which couldbe a major cause of recurrence of this disease in cattle.","palabras_clave":"CYSTIC OVARIAN DISEASE|BMP SYSTEM|OVARY|COW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5434,"idioma_id":4,"titulo":"Performance of a PCR assay for the rapid identi fi cation of the Klebsiella pneumoniae ST258 epidemic clone in Latin American clinical isolates","resumen":"The worldwide dissemination of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae ST258 demands a rapid PCR-based typing method to detect unique genes of the ST258 clone. This study evaluates a PCR developed by Adler et al. (2014) for the detection of ST258 in K. pneumoniae clinical isolates centered on the identification of the pilv-I and prp genes. We tested 143 clinical isolates from Argentina (n = 109), Chile (n = 1), Colombia (n = 1), Costa Rica (n = 2), Ecuador (n = 5), El Salvador (n = 2), Nicaragua (n = 5), Panamá (n = 2), Paraguay (n = 2), Perú (n = 3) and Trinidad and Tobago (n = 11) recovered from 2006 to 2015. blaKPC, pilv-l and prp genes were detected by PCR and sequenced by standard procedures. ST258 and non-ST258 were defined by PFGE and\/or MLST. Isolates were grouped according to PFGE patterns: 58 were compatible with ST258 (group 1) and 85 with non-ST258 (group 2). MLST study was done on an arbitrary selection of isolates. The pilv-l gene was present only in ST258 isolates, regardless of the presence of the blaKPC gene. Results for the prp gene were variable. Its presence did not define ST258. The pilv-I PCR had a sensitivity and specificity of 100%, respectively, for the detection of ST258 in the isolates under investigation. Given our findings, the pilv-I PCR could replace more time and resource consuming methods, allowing for more rapid detection of the circulating high risk K. pneumoniae clone ST258 in Latin American (LA) countries.","palabras_clave":"KPC|ST258|KLEBSIELLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5435,"idioma_id":5,"titulo":"ESTIMACIÓN DEL ÁREA CUBIERTA DE NIEVE CON DATOS SAR EN BANDA L EN UN SECTOR CORDILLERANO DE LA PATAGONIA ARGENTINA","resumen":"La estimación del área cubierta de nieve en cuencas andinas es importante para estudios hidrológicos y para una adecuada planificación del recurso agua en distintos usos como la generación hidroeléctrica, estudios de riesgo de inundación y atenuación de crecidas. Los sensores remotos ópticos e infrarrojos son una herramienta operativa para el monitoreo de la nieve en condicionesde adquisición con ausencia de nubosidad. Sin embargo, sólo los satélites que trabajan en el rango de las microondas son capaces de obtener datos de la superficie independientemente de las condiciones climáticas y de iluminación solar. En el presente trabajo se propone el desarrollo de una metodología para la generación de productos cartográficos de cuantificación del área cubierta de nieve y diferenciación de nieve húmeda y seca a partir de la utilización de técnicas de teleobservación óptica y de radar. Para ello se utilizaron datos del satélite de radar ALOS PALSAR en banda L y Landsat 5 TM y 7 ETM+ en un sector de la región cordillerana del norte de la Patagonia argentina que corresponde a la alta cuenca del río Aluminé. Los resultados se validaron con datos provenientes de sensores ópticos como también a partir de datos de temperatura de superficie.Utilizando los datos de radar se estimó la presencia de nieve en proceso de fusión pero no se pudo discriminar la presencia de nieve seca, dato que sí pudo ser obtenido con imágenes ópticas.","palabras_clave":"MONITOREO DE NIEVE|IMÁGENES DE RADAR|CUENCAS ANDINAS|TEMPERATURA DE SUPERFICIE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5436,"idioma_id":4,"titulo":"Spatio-temporal nitrogen fertilizer response in maize: field study and modeling approach","resumen":"Maize (Zea mays L.) yield and its response to nitrogen (N) are aff ected by the spatial variability of the interaction between weather, management, and soil properties. Th e objectives of this study were (i) to evaluate the response of spatial variability of maize yield by homogeneous zones (HZs) to diff erent N fertilizer rates under rainfed conditions, (ii) to test the ability of the SALUS (System Approach to Land Use Sustainability) model to simulate the eff ects of N rates on maize yield under rainfed and irrigated conditions, and (iii) to estimate spatial and temporal N fertilizer response risk in maize through the use of long-term simulations. In two fi eld experiments in Parana, Argentina (&#8722;31.8333°, &#8722;60.5167°) in 2011 (Field 1) and 2012 (Field 2), four fertilization treatments (0, 70, 140, and 210 kg ha&#8722;1) were evaluated in diff erent HZs. Th e SALUS model was used to evaluate spatial variability in yield, N response, and net revenue over the long-term period (1971?2012). Results showed that yield was signifi cantly aff ected by N rate (p < 0.01) in both fi elds and by HZ in Field 2 (p < 0.05), whereas N response was only aff ected by N rate. Simulated yield was signifi cantly aff ected by N. Th e model accounted for the spatial variability, showing HZ eff ect (p < 0.001) and a signifi cant HZ × N interaction (p < 0.0001). Th e optimal economic return N rate diff ered between HZs in both fi elds. Our procedure demonstrated the ability to improve N management by the selection of appropriate N rates across the fi eld, thereby improving N use effi ciency and growers? profi ts and reducing the potential for negative environmental impacts.","palabras_clave":"Maize|Nitrogen|Modeling|Homogenous zone"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5437,"idioma_id":4,"titulo":"Does urbanization affect the seasonal dynamics of bird communities in urban parks?","resumen":"The environmental factors affecting the spatial dynamics of bird communities in urban parks are well understood, but much less attention has been paid to the seasonal dynamics of bird communities. Since migrant and resident human commensal birds might have contrasting responses to environmental factors of urban parks, we expected different seasonal dynamics among parks. On the other hand, because bird species can have different habitat relationships throughout the year, we also expected different responses of bird richness to environmental variables between breeding and non-breeding seasons. Bird surveys were conducted in 14 small urban parks (1?4 Ha) of Mar del Plata city (Argentina) for one full annual cycle. Bird richness changed between seasons, but bird abundance remained constant. Bird community composition did not vary between seasons, but urban parks near the urban center, with the highest pedestrian traffic and isolation to other green areas had the least seasonal change of composition. During the breeding season, bird richness was negatively affected by the percentage cover of high buildings surrounding the immediate limits of parks, whereas during the non-breeding season bird richness was not related with any environmental variable. Bird composition variation among parks was affected by the distance to the urban center during both seasons. Results showed that urbanization promotes a seasonal homogenization of bird communities in urban parks, probably by affecting the presence of migrant species and promoting the temporal stability of human commensal species.","palabras_clave":"BIRD ABUNDANCE  |BIRD RICHNESS|BIRD COMPOSITION|BREEDING SEASON"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5438,"idioma_id":4,"titulo":"High-resolution images above the Pampean &#64258;at slab of Argentina (31?32&#9702;S) from local receiver functions: Implications on regional tectonics","resumen":"In the flat slab region of the South Central Andes (&#8764;31?32°S), geological observations suggest that the regional crustal structure is inherited from the accretion of different terranes during the Ordovician. These structures were later reactivated, first in extension during the Triassic and later in compression during the Andean uplift since the Miocene. Seismological observations confirmed that those fault structures extend to depth with décollement levels that accommodate current crustal shortening in the region. In order to get better insight on the regional tectonics we computed higher frequency receiver functions (RF) from local slab seismicity of intermediate &#8764;100 km depth. Using a common conversion point (CCP) stacking method we obtained cross sections showing high vertical resolution crustal structure at the transition between the Precordillera and the Frontal Cordillera. In addition we performed a joint inversion of our high frequency RFs with surface wave data from ambient noise tomography allowing us to constrain absolute seismic wave velocities. Our higher resolution images reveal more structural details down to a depth of 80 km and laterally over the flat slab in good agreement with previous studies. Our results help to better identify very shallow discontinuities in seismic velocities. Recent petrological analyses combined with our high-resolution RF structure correlates with a crustal mafic composition and partial eclogitization in the lower crust. We observe a shift in the crustal structure between the Precordillera (east) and the Frontal Cordillera (west). Regional seismicity and previously determined focal mechanisms superimposed over our images indicate this shifting is a thrust structure extending down to a depth of 40 km. Our results suggest the presence of a master fault between the Cuyania (Western Precordillera) and Chilenia (Frontal Cordillera) terranes that probably accommodates the crustal deformation in the Pampean flat slab region since the Late Ordovician.","palabras_clave":"ANDEAN RETROARC|SBDUCTION|BODY WAVES|ECLOGITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5439,"idioma_id":4,"titulo":"Megatherioidea (Mammalia, Xenarthra, Tardigrada) from the Pinturas Formation (Early Miocene), Santa Cruz Province (Argentina) and their chronological implications","resumen":"Pyroclastic and epiclastic continental sediments bearing the ??fauna Astrapothericulense?? from the Pinturas Formation of Ameghino crop out mainly at several localities at the upper valley of the Pinturas River and its tributaries, northwestern Santa Cruz Province, Argentina. These continental sediments are referred to the Burdigalian Stage and constitute the basis for the recognition of the Pinturas Formation. The fauna recorded in the Pinturas Formation mainly consists of mammals, specially rodents,native ungulates, xenarthrans and primates. Here wedescribe the first association of Megatherioidea (Mammalia, Xenarthra, Tardigrada) from different localities of the Pinturas Formation with accurate geographic and stratigraphic provenance. The Megatherioidea from the Pinturas Formation presented herein are represented by (1) Schismotherium cf. binum; (2) Hapalops sp.; and (3) a Megatherioidea indet. In addition, the holotypes of Schismotherium binum (MACN A 11750), Hapalops curvus (MACN A 11140), and Pelecyodon arcuatus were collected from the ??fauna Astrapothericulense?? of Ameghino; unfortunately, it is not easy to determine if they were collected from the Pinturas Formation since they are part of Ameghino?s Collection (MACN), but were collected a time when the Pinturas Formation had not yet been proposed. The presence of a species of Schismotherium andof Hapalops in the Pinturas Formation represent accurate early records for these genera, but not necessarily the earliest. The age of the Pinturas Formation could overlap, partially or totally, with the age of the Santa Cruz Formation at the Atlantic coast, where most Early Miocene Megatherioidea were collected.","palabras_clave":"DIVERSITY|NEOGENE|GROUND SLOTHS|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5440,"idioma_id":4,"titulo":"Analytical and rheological studies of modified gel dosimeters exposed to X-ray beams","resumen":"The need to know the dose of X-rays to be applied to patients suffering diseases such as cancerrequires accurate and stable dosimetric devices. Currently, the use of gelatin-based dosimetershas yielded excellent results but lack adequate thermal stability. In this paper a chemicalmodification of the gelatin (at concentrations typically used for the preparation of dosimeters)using glutaraldehyde as a cross-linking agent is proposed. Through rheological studies it wasfound that modified gelatin with glutaraldehyde concentrations between 0.15 and 0.50 % w \/ vshow better thermal stability with an increase in elastic modulus of up to 100 times at 37°C andconvenient reaction times for the preparation of the dosimeters. Subsequently, a mathematicalmodel to easily predict the elastic modulus of materials prepared with different concentrationsof gelatin and glutaraldehyde was proposed. The analytical response of modified andunmodified materials was evaluated and no significant alteration of the dosimetric sensitivitywas found in the dosimeters (based on itaconic acid and N, N´-methylenebisacrylamide) whenan X-ray irradiation dose from 0 to 300 Gy was applied. It was found that the best thermal  stability of dosimeters prepared with modified gelatin would decrease the loss of informationbetween the irradiation process and the absorbance reading, thereby improving the stability andlinear correlation of data.Overall, the results indicated that the dosimeters could be modified as proposed and achievesignificant improvements regarding to their thermal stability, without changing significantly theusual preparation process.","palabras_clave":"Dosimetry |Glutaraldehyde |X-Ray |Cross-Linking |Rheology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5441,"idioma_id":4,"titulo":"Tree age and bark thickness as traits linked to frost ring probability on Araucaria araucana trees in northern Patagonia","resumen":"Frost events may damage the cambium and consequently the newly produced tracheids whose cell walls have not yet completed their lignifications, leading to the formation of frost rings. This study deals with the presence of frost rings in Araucaria araucana trees according to cambial age and bark thickness,under the assumption that these factors may be involved in physical or physiological mechanisms that increase resistance to freezing temperatures that impact the cambial tissue. The study was conducted in northern Patagonia at two sites of contrasting geomorphology, and therefore potentially associated witha differential degree of exposure to extreme cold. Wood plus bark cores were extracted from main stems at two heights from the ground and from each of the four cardinal point directions for 30 individuals per site. A Linear Mixed Model and a Generalized Linear Mixed Model were applied in order to relatethe bark thickness and the frequency of frost rings in accordance with the different sampling points on the stem. It was observed that as bark becomes thicker with cambial age, the frequency of frost rings decreases, indicating a possible thermal-induced mechanism of bark protection. Consequently, there isan increase in the presence of frost rings at the younger stages of tree life. Although the mechanisms of cold hardiness in trees can be complex, including aspects of the tree physiology, our data indicated that as tree age increases, the thickness of the bark is higher, resulting in a potential effect of isolation and passive protection against the harmful effects of frosts. This mechanism may be relevant in the ecology, conservation and management of forests faced with extreme variability in future climate and changing scenarios.","palabras_clave":"Araucaria araucana |Bark |Cambial age| Frost ring|Thermal protection | Vascular cambium"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5442,"idioma_id":4,"titulo":"Preclinical platform of retinoblastoma xenografts recapitulating human disease and molecular markers of dissemination","resumen":"Translational research in retinoblastoma - a pediatric tumor that originates during the development of the retina - would be improved by the creation of new patient-derived models. Using tumor samples from enucleated eyes we established a new battery of preclinical models that grow in vitro in serum-free medium and in vivo in immunodeficient mice. To examine whether the new xenografts recapitulate human disease and disseminate from the retina to the central nervous system, we evaluated their histology and the presence of molecular markers of dissemination that are used in the clinical setting to detect extraocular metastases. We evaluated GD2 synthase and CRX as such markers and generated a Taqman real-time quantitative PCR method to measure CRX mRNA for rapid, sensitive and specific quantification of local and metastatic tumor burden. This approach was able to detect 1 human retinoblastoma cell in 100.000 mouse brain cells. Our research adds novel preclinical tools for the discovery of new retinoblastoma treatments for clinical translation.","palabras_clave":"CRX|METASTASIS| RETINOBLASTOMA|XENOGRAFT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5443,"idioma_id":4,"titulo":"Variation in camelid d13C and d15N values in relation to geography and climate: Holocene patterns and archaeological implications in central western Argentina","resumen":"Camelids are among the largest wild and domestic faunas in South America and represent one of the most important taxa to pre-hispanic South America human populations. Stable isotope data from these animals play an important role in improving our understanding of human paleodiet, past human-animal interactions, Holocene environmental change, and modern camelid management. This paper resentsd13 C andd 15 N values taken from 91 camelid specimens distributed across western Argentina between 30 and 37 S. These samples come from three desert environments (Andean, Patagonia, and Monte) and include both modern and prehistoric samples. Camelidd 13 C values range between20.3?and10.7?, whiled15 N values vary between 2?and 10.2?. Mean isotope values differ by environmental context, with significant difference ind 13 C andd 15 N between Patagonian and Monte or Andean deserts. Camelid isotope values also vary with latitude, altitude and longitude, though differences in d 15 N are weak, and these geographic differences are tied to climatic variables such as annual mean temperature, annual precipitation, and season of precipitation. When comparing camelidd 13 C values from Central-western Argentina with those from Northwest Argentina and Patagonia, we see a latitudinal trend of decreasingd 13 C values, with the most negative values occurring in southern Patagonia and the mostpositive values in Northwest Argentina. Variation in camelid stable isotope values and their association with particular environmental contexts shows their value as a geographic marker and possibly as a paleoecological proxy. These results highlight the need to consider the geographic origin of camelidisotope values when using them to reconstruct human diet.","palabras_clave":"camelids|stable isotopes|central-western argentina|Holocene ecology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5444,"idioma_id":4,"titulo":"SN-38-loaded nanofiber matrices for local control of pediatric solid tumors after subtotal resection surgery","resumen":"In addition to surgery, local tumor control in pediatric oncology requires new treatments as an alternative to radiotherapy. SN-38 is an anticancer drug with proved activity against several pediatric solid tumors including neuroblastoma, rhabdomyosarcoma and Ewing sarcoma. Taking advantage of the extremely low aqueous solubility of SN-38, we have developed a novel drug delivery system (DDS) consisting of matrices made of poly(lactic acid) electrospun polymer nanofibers loaded with SN-38 microcrystals for local release in difficult-to-treat pediatric solid tumors. To model the clinical scenario, we conducted extensive preclinical experiments to characterize the biodistribution of the released SN-38 using microdialysis sampling in vivo. We observed that the drug achieves high concentrations in the virtual space of the surgical bed and penetrates a maximum distance of 2 mm within the tumor bulk. Subsequently, we developed a model of subtotal tumor resection in clinically relevant pediatric patient-derived xenografts and used such models to provide evidence of the activity of the SN-38 DDS to inhibit tumor regrowth. We propose that this novel DDS could represent a potential future strategy to avoid harmful radiation therapy as a primary tumor control together with surgery.","palabras_clave":"Pediatric|Oncology|SN-38|Irinotecan"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5445,"idioma_id":4,"titulo":"ROLE OF LANDSCAPE ELEMENTS ON RECENT DISTRIBUTIONAL EXPANSION OF EUROPEAN STARLINGS (STURNUS VULGARIS) IN AGROECOSYSTEMS OF THE PAMPAS, ARGENTINA","resumen":"Previous studies of European Starlings in Argentina have focused on identifying biological aspects correlated with establishment of new populations in urban and suburban areas. Starlings have recently invaded rural areas in the Pampas. To understand the factors involved in the recent expansion of European Starlings into these rural habitats, we investigated how expansion patterns were associated with season, proportion of crop fields, distance to woodlots, and distance to small and big urban centers in agroecoystems of the Pampas. We surveyed 392 fields during 2011?2013 to collect data on presence of starlings and landscape features. We found that the range of European Starlings has expanded by a total area of ,65,000 km2 since 2005, at a linear range expansion rate of 22.2 km per year. Generalized linear mixed model analysis revealed that presence of European Starlings was significantly related with reduced distances to nearest small urban area. Our findings indicate that range expansion of European Starlings into rural areas of Argentina may follow a neighborhood diffusion pattern, by which well-established populations act as sources of individuals that disperse short distances into nearby favorable areas. In absence of human control, this species is expected to continue its spread and population increase.","palabras_clave":"AGRICULTURE|BIOGEOGRAPHY|BIOLOGICAL INVASIONS|NEOTROPICS|STURNIDAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5446,"idioma_id":4,"titulo":"Sensing membrane thickness: Lessons learned from cold stress","resumen":"The lipid bilayer component of biological membranes is important for the distribution, organization, and function of bilayer spanning proteins. These physical barriers are subjected to bilayer perturbations. As a consequence, nature has evolved proteins that are able to sense changes in the bilayer properties and transform these lipid-mediated stimuli into intracellular signals. A structural feature that most signal-transducing membrane-embedded proteins have in common is one or more &#945;-helices that traverse the lipid bilayer. Because of the interaction with the surrounding lipids, the organization of these transmembrane helices will be sensitive to membrane properties, like hydrophobic thickness. The helices may adapt to the lipids in different ways, which in turn can influence the structure and function of the intact membrane proteins. We review recent insights into the molecular basis of thermosensing via changes in membrane thickness and consider examples in which the hydrophobic matching can be demonstrated using reconstituted membrane systems. This article is part of a Special Issue entitled: The cellular lipid landscape edited by Tim P. Levine and Anant K. Menon.","palabras_clave":"Membrane thickness|Transmembrane signaling|Thermosensing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5447,"idioma_id":4,"titulo":"Distribution of rDNA and polyploidy in Deschampsia antarctica E. Desv. in Antarctic and Patagonic populations","resumen":"Unlike the Arctic flora, with many flowering plant species offering opportunities to study evolutionary processes, the Antarctic flora offers only two. One of them is the Antarctic grass Deschampsia antarctica E. Desv., whose distribution spans from northern Patagonia (ca. 38°S) down to Alamode Island (ca. 68°S), in the west side of the Antarctic Peninsula. While some aspects of Antarctic plants have been extensively studied (e.g., anatomy, physiology, genetics), little is known about the related Patagonian populations. Particularly in cytogenetics, no single study has focused on continental populations and its relationships with the Antarctic plants. The combination of traditional fluorescent in situ hybridization (FISH) with a phylogenetic framework highlights the importance of cytogenetics in plant evolutionary studies, by allowing comparison of chromosome characters in phylogenetically related individuals. Most used characters for this purpose are the chromosome number, karyotype morphology and patterns of repetitive DNA. These were used to compare distant populations of D. antarctica in a phylogenetic framework, to obtain a first view of the cytogenetic structure of the species along its distribution. Patagonian populations have greater variability in the chromosomal and molecular characters, while Antarctic populations are very alike, hinting at a South American origin hypothesis. A polyploid population is reported for the first time, located on Central Patagonia populations, close to the northern limit of distribution range. Cytogenetic characteristics suggest that hybridization processes could have played an important role in the evolution of the genome of D. antarctica.","palabras_clave":"Deschampsia antarctica|Karyotype|FISH |Highly repetitive DNA|Polyploidy |Phylogeny"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5448,"idioma_id":4,"titulo":"Dry reforming of Methane on a highly-active Ni-CeO2 catalyst: Effects of metal-support interactions on C-H bond breaking","resumen":"Ni-CeO2 is a highly efficient, stable and non-expensive catalyst for methane dry reforming at relative low temperatures (700 K). The active phase of the catalyst consists of small nanoparticles of nickel dispersed on partially reduced ceria. Experiments of ambient pressure XPS indicate that methane dissociates on Ni\/CeO2 at temperatures as low as 300 K, generating CHx and COx species on the surface of the catalyst. Strong metal-support interactions activate Ni for the dissociation of methane. The results of density-functional calculations show a drop in the effective barrier for methane activation from 0.9 eV on Ni(111) to only 0.15 eV on Ni\/CeO1-x(111). At 700 K, under methane dry reforming conditions, no signals for adsorbed CHx or C species are detected on the C 1s XPS region. The reforming of methane proceeds in a clean and efficient way.","palabras_clave":"ceria|density functional theory|methane dissociation|nickel|X-ray photoelectron spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5449,"idioma_id":4,"titulo":"Dynamic regulation of cell volume and extracellular ATP of human erythrocytes","resumen":"IntroductionThe peptide mastoparan 7 (MST7) triggered in human erythrocytes (rbcs) the release of ATP and swelling. Since swelling is a well-known inducer of ATP release, and extracellular (ATPe), interacting with P (purinergic) receptors, can affect cell volume (Vr), we explored the dynamic regulation between Vr and ATPe.Methods and TreatmentsWe made a quantitative assessment of MST7-dependent kinetics of Vr and of [ATPe], both in the absence and presence of blockers of ATP efflux, swelling and P receptors.ResultsIn rbcs 10 &#956;M MST7 promoted acute, strongly correlated changes in [ATPe] and Vr. Whereas MST7 induced increases of 10% in Vr and 190 nM in [ATPe], blocking swelling in a hyperosmotic medium + MST7 reduced [ATPe] by 40%. Pre-incubation of rbcs with 10 &#956;M of either carbenoxolone or probenecid, two inhibitors of the ATP conduit pannexin 1, reduced [ATPe] by 40?50% and swelling by 40?60%, while in the presence of 80 U\/mL apyrase, an ATPe scavenger, cell swelling was prevented. While exposure to 10 &#956;M NF110, a blocker of ATP-P2X receptors mediating sodium influx, reduced [ATPe] by 48%, and swelling by 80%, incubation of cells in sodium free medium reduced swelling by 92%.Analysis and DiscussionResults were analyzed by means of a mathematical model where ATPe kinetics and Vr kinetics were mutually regulated. Model dependent fit to experimental data showed that, upon MST7 exposure, ATP efflux required a fast 1960-fold increase of ATP permeability, mediated by two kinetically different conduits, both of which were activated by swelling and inactivated by time. Both experimental and theoretical results suggest that, following MST7exposure, ATP is released via two conduits, one of which is mediated by pannexin 1. The accumulated ATPe activates P2X receptors, followed by sodium influx, resulting in cell swelling, which in turn further activates ATP release. Thus swelling and P2X receptors constitute essential components of a positive feedback loop underlying ATP-induced ATP release of rbcs.","palabras_clave":"Hematocrit|Permeability|Red blood cells|Mathematical models|Anions|Simulation and modeling|Capillary electrophoresis|Cholines"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5450,"idioma_id":4,"titulo":"From Modern Push-Button Hospital-beds to 20th Century Mechatronic Beds: A Review","resumen":"The aim of this work is to present the different aspects of modern high complexity electric beds of the period 1940 until 2000 exclusively. The chronology of the product has been strictly divided into three big stages: electric and semi-electric beds (until the 90?s), mechatronic beds (90?s until 2000) and, mechatronic intelligent beds of the last 15 years. The latter are not considered in this work due to the extension for its analysis. The justification for classifying the product is presented under the concepts of medical, assistive and mobility devices. Relevant aspects of common immobility problems of the different types of patients for which the beds are mainly addressed are shown in detail. The basic functioning of the patient?s movement generator and the implementation of actuators, together with IT programs, specific accessories and connectivity means and network-communication shown in this work, were those that gave origin to current mechatronic beds. We present the historical evolution of high complexity electric beds by illustrating cases extracted from a meticulous time line, based on patents, inventions and publications in newspapers and magazines of the world. The criteria adopted to evaluate the innovation were: characteristics of controls; accessories (mattresses, lighting, siderails, etc.), aesthetic and morphologic properties and outstanding functionalities.","palabras_clave":"PUSH-BUTTON BED|MECHATRONIC BED|MEDICAL DEVICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5451,"idioma_id":4,"titulo":"Polyelectrolytes multilayers to modulate cell adhesion: A study of  the influence of film composition and polyelectrolyte interdigitation on the adhesion of the A549 cell line","resumen":"Polyelectrolyte multilayers (PEMs) with different polycation\/polyanion pairs are fabricated by the layer-by-layer technique employing synthetic, natural, and both types of polyelectrolytes. The impact of the chemical composition of PEMs on cell adhesion is assessed by studying cell shape, spreading area, focal contacts, and cell proliferation for the A549 cell line. Cells exhibit good adhesion on PEMs containing natural polycations and poly(sodium 4-styrenesulfonate) (PSS) as polyanion, but limited adhesion is observed on PEMs fabricated from both natural polyelectrolytes. PEMs are then assembled, depositing a block of natural polyelectrolytes on top of a stiffer block with PSS as polyanion. Cell adhesion is enhanced on top of the diblock PEMs compared to purely natural PEMs. This fact could be explained by the interdigitation between polyelectrolytes from the two blocks. Diblock PEM assembly provides a simple means to tune cell adhesion on biocompatible PEMs. Biocompatible surfaces with improved cell adhesion are obtained by diblock polyelectrolyte multilayers (PEMs). On the basis of cell adhesion studied on a large number of PEMs made of natural and synthetic polyelectrolytes, a diblock PEM is fabricated by combining a block of soft natural polyelectrolytes on the top of a stiffer block containing poly(sodium 4-styrenesulfonate) as polyanion.","palabras_clave":"xx"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5452,"idioma_id":4,"titulo":"Invasion of rural houses by wild Triatominae in the arid Chaco","resumen":"Triatomines are the vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease, the main endemic disease affecting five to seven million people in Latin America. Besides Triatoma infestans, the most important T. cruzi vector in the Gran Chaco region, other triatomine species associated with sylvatic birds and mammals are responsible for the maintenance of the wild cycle of T. cruzi. The present study aimed at evaluating the house invasion by sylvatic triatomine species in rural communities of the Los Llanos region (La Rioja, Argentina) and its association with environmental variables. House invasion by flying adult triatomines was recorded by trained collectors that surveyed over 377 houses distributed over 73 localities in a 56,600 km2 study region, between October, 2014 and February, 2015. The result of the study showed the frequent house invasion by adult triatomines: 26.3% houses were infested in 53% of the localities. Seven sylvatic triatomine species were collected, with T. guasayana and T. garciabesi among the most abundant. House invasion by triatomine species showed no spatial aggregation and was not associated with temperature, precipitation, or vegetation cover at the spatial scale considered in the present study. House invasion by the epidemiologically important T. infestans is a concern of rural communities. Besides constituting a latent, although low, risk, the presence of these species negatively interferes with the vigilance activities of the provincial Chagas disease program. Journal of Vector Ecology 41 (1): 97-102. 2016.","palabras_clave":"TRIATOMA GUASAYANA|TRIATOMA GARCIABESI|ENVIRONMENTAL VARIBLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5453,"idioma_id":4,"titulo":"Preparation and characterization of soy lecithin-modified bentonites","resumen":"In this work, a study of exchange of soy lecithin, a natural product, in bentonite was performed in order to synthesizebio-organoclays. The effects of initial amount of modifier and reaction time were studied at a fixed reactiontemperature. Organoclays thus obtainedwere characterized by means of X-ray diffraction (XRD) analysis, Xrayfluorescence spectrometry (XRF), thermogravimetric analysis (TGA), Fourier transforminfrared spectroscopy(FTIR) and water absorption tests. An effective intercalation of soy lecithin between the clay layerswas obtained.The ionic exchange reaction was completed at short times whereas variations in the initial amount of modifierproduced organoclayswith different final properties. At lowratios of soy lecithin to bentonite, a slight incrementin basal spacing of organoclays was observed due to intercalation of the organic modifier between the clay layersand a significant diminution on water absorption was achieved. When the organic content increased, the interlayerspacing increased but thermal stability of organoclays decreased compared to the sampleswith loworganiccontent, whereas the water absorption was not affected. The obtained bio-organoclays are potentialenvironmental-friendly fillers for the development of clay\/biopolymer nanocomposites.","palabras_clave":"Organoclay|Soy lecithin|Ionic exchange|Characterization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5454,"idioma_id":5,"titulo":"Avances en las inmunoterapias para alergias alimentarias","resumen":"&nbsp;Lasenfermedades alérgicas son las inmunopatologías que con mayor preva­lencia sepresentan en el mundo. Pueden o no estar mediadas por anticuer­pos IgE, sinembargo estas últimas son las que más intensamente se han estudiado por elriesgo que presentan para la vida del paciente. Si bien el único tratamientoque logra revertir estos mecanismos es la no exposición al alergeno, esto nosiempre es posible. Por esta razón, y a partir del mayor conocimiento alcanzadodel sistema inmune de mucosas junto al desarrollo de modelos animales dealergia, existe un marcado interés en la especiali­dad para el desarrollo deinmunoterapias que controlen y reviertan el estado de alergia. A partir de losensayos pre-clínicos en animales y la aplicación de protocolos terapéuticos enensayos clínicos, se han desarrollado tera­pias mucosales que logran inducirmecanismos de tolerancia específicos del alergeno, los cuales son capaces derevertir la sensibilización alérgica. Dado que el principal escollo siguensiendo las reacciones adversas inducidas durante el tratamiento, se requiereprofundizar los estudios para desarrollar protocolos terapéuticos más seguros.En este punto la medicina traslacional encuentra un campo próspero parafortalecer las interacciones entre la cien­cia básica, la aplicada y la clínica","palabras_clave":"alergia|inmunoterapias|inmunidad de mucosas|IgE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5455,"idioma_id":4,"titulo":"Copper(II) complexes with saccharinate and glutamate as antitumor agents. Cyto- and genotoxicity in human osteosarcoma cells","resumen":"We report herein the antitumor actions of three copper (II) complexes on MG-63 human osteosarcoma cells. The three complexes: Cu-sac, Cu-gln and Cu-sac-gln (sac= saccharinate, gln= glutamine) caused a decline in cell viability. The half-maximal inhibitory concentration in MG-63 cells for Cu-sac-gln is 170 &#956;M, showing the strongest antiproliferative effect. Moreover, only Cu-sac-gln caused a decrease in the mitochondrial activity from 100 &#956;M. Our results indicate that the copper (II) complexes studied here produce DNA damage and suggest that the rise of reactive oxygen species (ROS) is the central mechanism action. Genotoxicity studied by the Cytokinesis-block micronucleus (MN) assay and the Single cell gel electrophoresis (comet assay) could be observed in MG-63 cells treated with Cu-sac-gln from 100 and 50 &#956;M, respectively. Cu-sac and Cu-gln also induced DNA damage; however their effect was definitively weaker. The generation of reactive oxygen species increased from 50 &#956;M of Cu-sac-gln and Cu-sac and only from 250 &#956;M of Cu-gln, as well as a reduction of the GSH\/GSSG ratio from 50 &#956;M. When cells were treated with several concentrations of the complexes in addition to a combination of 50 &#956;M of vitamin C plus 50 &#956;M of vitamin E, a total recovery in cell survival was obtained for Cu-gln in the whole range of tested concentrations while only a partial viability recovery was obtained from 250 &#956;M of Cu-sac and Cu-sac-gln. Overall, our results point to a differential cyto-and genotoxicity of the three copper (II) complexes and demonstrate that the complexation with both ligands confers the most potent antitumor action in human osteosarcoma cells.","palabras_clave":"COPPER(II) COMPLEXES|ANTITUMOR DRUGS|CYTOTOXICITY|GENOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5456,"idioma_id":4,"titulo":"Hydrophobic channels produced by micelle-structured CTAB inside MCM-41 mesopores: A unique trap for the hazardous hormone ethinyl estradiol","resumen":"In this work, mesoporous hydrophobic cavities produced by a micelle structured CTAB (cetyl trimethylammonium bromide) trapped inside MCM-41 pores were used for the efficient removal of the hazardous hormone contaminant ethinyl estradiol (EE) present in water. The MCM-41 structure was prepared using different CTAB\/Si molar ratios, i.e. 0.07, 0.12, 0.17, and 0.27. TG, SEM, TEM, BET, IR, elemental analysis, contact angle and positron annihilation spectroscopy showed that the MCM-41 silica structure is completely filled with trapped CTAB molecules (47?58 wt%) which results in no porosity and very low surface areas of 1?5 m2 g 1. These materials showed high efficiency to remove EE from aqueous solution whereas pure MCM-41 (without CTAB) with high porosity and a surface area of 1333 m2 g 1 showed no adsorption. These results are discussed in terms of EE sorption into the hydrophobic environment created by the CTAB trapped molecules inside the mesoporous cavities of MCM-41.","palabras_clave":"MCM-41|HYDROPHOBIC ENVIRONMENT|TRAP FOR ETHINYL ESTRADIOL|MESOPOROUS CAVITIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5457,"idioma_id":4,"titulo":"New insights on estimating pore size distribution of latex particles: Statistical mechanics approach and modeling","resumen":"New statistical mechanics approach for the pore size distribution applied in a wide relative pressure range is proposed. The new proposed model was applied to nitrogen adsorption-desorption isotherms at 77K onto five functionalized polystyrene latices. Results showed that the proposed model can reproduce all results found by traditional methods such as NLDFT, BJH and VBS where some of them can be applied only for a specific range of pore size. A segmentation procedure is adopted and it is shown that the corresponding algorithm can be successfully applied for determining pore size distributions over a wide range of pore size. When this method is applied isotherms Type II and III (materials with larger mesopores and\/or macropores) gives additional information that is not obtained with the other methods. The obtained results showed that the copolymerization plays an important role in the porosity and the specific surface area, whereas, the high polydispersity index, PDI, can reduce the porosity. The samples studied within the present work present small and large mesopores and even macropores as it is suggested by the new proposed model, and part of this porosity could be related to the interparticle and also to the intraparticle porosity.","palabras_clave":"STATISTICAL MECHANICS APPROACH|PORE SIZE DISTRIBUTION|SORPTION MODELING|NITROGEN ADSORPTION-DESORPTION ISOTHERMS|LATEX PARTICLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5458,"idioma_id":4,"titulo":"An ordered mesoporous carbon modified electrochemical sensor for solid-phase microextraction and determination of triclosan in environmental samples","resumen":"A simple and sensitive electrochemical sensor was performed with an ordered mesoporous carbon modified screen-printed carbon electrode (SPCE) to detect triclosan (TCS) in river water samples. A nanostructured mesoporous carbon CMK-3 type was successfully obtained from ordered mesoporous silica SBA-15 type used as hard-templated and sucrose as carbon precursor. Due to its high specific surface area (1125 m2 g-1), large pore volume (1.16 cm3 g-1), uniform mesostructure (4.4 nm), good conductivity and excellent electrochemical activity, this porous carbon material provides selectivity and sensitivity forthe electrochemical determination. CMK-3 was immobilized onto a SPCE using chitosan (CH) as a binder. CMK-3\/CH\/SPCE was characterized by cyclic voltammetry, transmission electron microscopy, scanning electron microscopy, X-ray diffraction, energy dispersive spectrometry and N2 adsorption-desorption isotherms.CMK-3\/CH was used for solid-phase microextraction, since it has an excellent extraction selectivity towards organic aromatic compounds. The electrochemical behavior of TCS showed an irreversible oxidation peak measured by square wave voltammetry. The detection limit of this electrochemical sensor was 0.24 ng mL-1 with a wide linear range from 0.8 ng mL-1 to 40 ng mL-1, the intra- and inter-assay coefficients of variation were below 4%. This electrochemical platform offered a useful tool for on-site TCS determination in environmental samples.","palabras_clave":"ELECTROCHEMICAL SENSOR|SOLID-PHASE MICROEXTRACTION|ORDERED MESOPOROUS CARBON|TRICLOSAN|ENVIRONMENTAL MONITORING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5459,"idioma_id":4,"titulo":"Influence of different factors on the particle size distribution and solid fat content of water-in-oil emulsions","resumen":"The influence of the variation of differentparameters on the particle size distribution and solid fatcontent (SFC) of water-in-oil emulsions was studied. Theuse of solid fat instead of liquid oil, higher polyglycerolpolyricinoleate (PGPR) concentration, or higher homogenizationenergy led to smaller mean particle sizes. Thedecrease of the emulsifier\/water ratio turned the particlesize distribution of the emulsions from bimodal to trimodal.The increase of PGPR concentration increased the SFC ofthe fat in the absence of water, but it did not produce thesame effect in the emulsion. This result suggested that thepresence of dispersed aqueous phase prevented the modifyingaction of the emulsifier on the crystallization of thecontinuous lipid phase. The experimentation indicatedthat the adsorption of the surfactant at the interface wouldreduce its availability to affect crystallization in bulk fat, asthe SFC in lipid phase decreased with increasing interfacialarea.","palabras_clave":"W\/O EMULSIONS |PARTICLE SIZE DISTRIBUTION|SOLID FAT CONTENT |PGPR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5460,"idioma_id":4,"titulo":"The 1H NMR spectrum of pyrazole in a nematic phase","resumen":"The experimental 1H nuclear magnetic resonance (NMR) spectrum of 1H-pyrazole was recorded in thermotropic nematic liquid crystal N-(p-ethoxybenzylidene)-p-butylaniline (EBBA) within the temperature range of 299?308 K. Two of three observable dipolar DHH-couplings appeared to be equal at each temperature because of fast prototropic tautomerism. Analysis of the Saupe orientational order parameters using fixed geometry determined by computations and experimental dipolar couplings results in a situation in which the molecular orientation relative to the magnetic field (and the liquid crystal director) can be described exceptionally by a single parameter.","palabras_clave":"D couplings|1H-pyrazole|DFT calculations|tautomerism|orientational order"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5461,"idioma_id":5,"titulo":"Primeras aproximaciones a la identificación de técnicas de procesamiento en carporrestos de Zea mays L.: detección de granos hervidos en restos arqueobotánicos secos y carbonizados","resumen":"Restos arqueológicos de maíz (Zea mays L.) han sido encontrados en diversos contextos arqueológicos, ya seaen forma de macro- o de microrrestos, a lo largo de gran parte de la historia prehispánica del Noroeste argentino.Diversos estudios experimentales y arqueobotánicos se han realizado procurando detectar procesamientoen este tipo de restos. A pesar de lo anterior, no se cuenta, para el caso del maíz, con estudios que abordenlas alteraciones que el procesamiento ocasiona en la anatomía de los granos, tanto en su nivel tisular, comocelular y subcelular. El objetivo del presente trabajo es contribuir a la identificación de una técnica de procesamiento,el hervido de granos enteros, mediante estudios experimentales y el análisis de caracteres cualitativosy cuantitativos a nivel anatómico y morfológico que puedan ser aplicados al estudio de restos arqueológicossecos y carbonizados. Como resultado se presentan una serie de rasgos que se asocian al hervido de los granosy su aplicación a un caso arqueológico. Finalmente, se discute el alcance de los estudios llevados a cabocomo parte de una primera aproximación a esta línea de análisis que se avizora como muy prometedora parael estudio de prácticas culinarias pasadas.","palabras_clave":"Maiz|Procesamiento|Experimentación|Arqueobotanica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5462,"idioma_id":4,"titulo":"Total Polyphenol Extraction From Red Sorghum Grain And Effects On The Morphological Structure Of Starch Granules","resumen":"The aim of this study was to propose a treatment to reduce the content of total polyphenols (TPP) and to obtain flour without significant morphological changes in the starch granules. The response surface methodology (RSM) was used to predict the influence of temperature and time on TPP extraction. Research work was conducted using the central composite design methodology. Results showed that the effect of temperature was more significant than that of time. A quadratic fit of data regarding temperature and linear data adjustment as to time was obtained. A scanning electron microscopy analysis of starch showed no morphological change in the granules at the temperature studied. Starch granules presented a size distribution ranging from 6 to 25 &#956;m being 35% of granules 15 &#956;m in size, respectively.","palabras_clave":"ANTIOXIDANT CAPACITY|FLOUR|NUTRIENT UTILIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5463,"idioma_id":4,"titulo":"Biomechanics and Biotensegrity: Study Method and Frequency Response of the Simplex and 3-bar-SVD Tensegrity Configurations","resumen":"The purpose of this work is to study the frequency response of 3D tensegrity structures. These are structures that have been used, since the 80?s, to model biological systems of different scales. This fact led to the origin of the field of biotensegrity, which includes biomechanics as a natural field of application. In this work: a) A simple method for the analysis of frequency response of different nodes in 3D tensegrity structures was set up and tuned. This method is based on a video-analysis algorithm, which was applied to the structures, as they were vibrated along their axis of symmetry, at frequencies from 1 Hz to 60 Hz. b) Frequency-response analyses were performed, for the simplest 3D structure, the Simplex module, as well as for two towers, formed by stacking two and three Simplex modules, respectively. Resonant frequencies were detected for the Simplex module at (19.2± 0.1) Hz and (50.2±0.1) Hz (the latter being an average of frequencies between homologous nodes). For the towers with two and three modules, each selected node presented a characteristic frequency response, modulated by their spatial placement in each model. Resonances for the two-stage tower were found at: (12 ±0.1) Hz; (16.2±0.1) Hz; (29.4±0.1) Hz and (37.2 ± 0.1) Hz. For the tower with three Simplex modules, the main resonant frequencies were found at (12.0± 0.1) Hz and (21.0± 0.1) Hz. Results show that the proposed method is adequate for the study (2D) of any 3D tensegrity structure, with the potential of being generalized to the study of oscillations in three dimensions. A growing complexity and variability in the frequency response of the nodes was observed, as modules were added to the structures. These findings were compared to those found in the available literature.","palabras_clave":"BIOMECHANICS|BIOTENSEGRITY|FREQUENCY|SIMPLEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5464,"idioma_id":4,"titulo":"Phenolic acid protects of renal damage induced by ochratoxin A in a 28-days-oral treatment in rats","resumen":"The present study aimed to characterize the chlorogenic acid (ChlA) capacity to reverse the toxic effects induced by ochratoxin A (OTA) in a subacute toxicity test in rats. Male Wistar rats were fed orally by gavage for 28 days with OTA (0.4 mg\/kg bw\/day), ChlA (5 mg\/kg bw\/day) or the combination OTA (0.4 mg\/kg bw\/day) + ChlA (5 mg\/kg bw\/day). No deaths, no decrease in feed intake or body weightin any experimental group were recorded. The negative control group and the animals treated with ChlA alone showed no changes in any parameters evaluated. In OTA-treated group significant changes such as decrease in urine volume, proteinuria, occult blood, increase in serum creatinine values; decrease in absolute and relative kidney weight and characteristics histopathological lesions thatindicated kidney damage were observed. However, limited effect on oxidative stress parameters were detected in kidneys of OTA-treated group. Animals treated with the combination OTA+ ChlA were showed as negative control group in the evaluation of several parameters of toxicity. In conclusion, ChlA, at given concentration, improved biochemical parameters altered in urine and serum and pathological damages in kidneys induced by OTA exposure, showing a good protective activity, but not by an apparent antioxidant mechanism.","palabras_clave":"OCHRATOXIN A |KIDNEY DAMAGE |CHLOROGENIC ACID |ORAL TREATMENT |PROTECTIVE EFFECTS |OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5466,"idioma_id":4,"titulo":"Discrete Kalman Filter based Sensor Fusion for Robust Accessibility Interfaces","resumen":"Human-machine interfaces have evolved, benefiting from the growing access to devices with superior, embedded signal-processing capabilities, as well as through new sensors that allow the estimation of movements and gestures, resulting in increasingly intuitive interfaces. In this context, sensor fusion for the estimation of the spatial orientation of body segments allows to achieve more robust solutions, overcoming specific disadvantages derived from the use of isolated sensors, such as the sensitivity of magnetic-field sensors to external influences, when used in uncontrolled environments. In this work, a method for the combination of image-processing data and angular-velocity registers from a 3D MEMS gyroscope, through a Discrete-time Kalman Filter, is proposed and deployed as an alternate user interface for mobile devices, in which an on-screen pointer is controlled with head movements. Results concerning general performance of the method are presented, as well as a comparative analysis, under a dedicated test application, with results from a previous version of this system, in which the relative-orientation information was acquired directly from MEMS sensors (3D magnetometer-accelerometer). These results show an improved response for this new version of the pointer, both in terms of precision and response time, while keeping many of the benefits that were highlighted for its predecessor, giving place to a complementary method for signal acquisition that can be used as an alternative-input device, as well as for accessibility solutions.","palabras_clave":"DISCRETE-KALMAN FILTER|SENSOR FUSION|MEMS|GYROSCOPE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5467,"idioma_id":4,"titulo":"Analysis of the genetic ancestry of patients with oral clefts from South American admixed populations","resumen":"Increased susceptibility to cleft lip, with or without cleft palate (CL±P) has been observed in South America, as related to Amerindian ancestry, using epidemiological data, uniparental markers, and blood groups. In this study, it was evaluated whether this increased risk remains when Amerindian ancestry is estimated using autosomal markers and considered in the predictive model. Ancestry was estimated through genotyping 62 insertion and deletion (INDEL) markers in sample sets of patients with CL±P, patients with cleft palate (CP), and controls, from Patagonia in southern Argentina and Belém in northern Brazil. The Amerindian ancestry in patients from Patagonia with CL±P was greater than in controls although it did not reach statistical significance. The European ancestry in patients with CL±P from Belém and in patients with CP from Belém and Patagonia was higher than in controls and statistically significant for patients with CP who were from Belém. This high contribution of European genetic ancestry among patients with CP who were from Belém has not been previously observed in American populations. Our results do not corroborate the currently accepted risks for CL±P and CP estimated by epidemiological studies in the North American populations and probably reflect the higher admixture found in South American ethnic groups when compared with the same ethnic groups from the North American populations.","palabras_clave":"INDEL|South American populations|cleft lip and palate; cleft palate|genetic ancestry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5469,"idioma_id":5,"titulo":"Relevamiento de Plagas en el Cinturón Hortícola de Bahía Blanca.","resumen":"El Cinturón Hortícola de Bahía Blanca, está localizado en el valle inferior del río Sauce Chico. Posee una superficie de 700 has y está integrado por distintos núcleos y su producción se comercializa en la ciudad de Bahía Blanca y su zona de influencia. La lechuga, Lactuca sativa, ocupa un lugar preponderante dentro de este cinturón hortícola con un área productiva de aproximadamente 60 Ha. Dada la importancia de esta hortaliza en la región, el conocimiento de los factores adversos que afectan su calidad y rendimiento son prioritarios. Dentro de los factores bióticos diversos insectos plaga desvalorizan al producto en el momento de la comercialización. En este contexto, resultó de interés registrar durante el período 2010-2012 la abundancia y la riqueza específica de los insectos plaga presentes en el cultivo de lechuga de diferentes establecimientos del cinturón hortícola. Durante el periodo 2010-2011 las especies más preponderantes fueron Myzus persicae, Nasonobia ribisnigri e Hyperomyzus lactucae. Durante el periodo 2011-2012 se observó la presencia de Myzus persicae, Macrosiphum euphorbiae, Hyperomyzus lactucae, Nasonobia ribisnigri y Uroleucon sonchi. Este estudio constituye un importante aporte en cuanto a la presencia y momento de aparición de las especies plaga presentes en el cultivo de Lactuca sativa en el cinturón hortícola de Bahía Blanca.","palabras_clave":"AFIDOS  |LACTUCA SATIVA|CINTURON HORTICOLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5470,"idioma_id":4,"titulo":"Non-constant ponderomotive energy in above threshold ionization by intense short laser pulses","resumen":"We analyze the contribution of the quiver kinetic energy acquired by an electron in an oscillating electric field of a short laser pulse to the energy balance in atomic ionization processes. Due to the time dependence of this additional kinetic energy, a temporal average is assumed to preserve a stationary energy conservation rule, which is used to predict the position of the energy peaks observed in the photoelectron (PE) spectra. For a plane wave and a flattop pulse, the mean value of the quiver energy over the whole pulse leads to the concept of ponderomotive energy Up. However, for a short pulse with a fast changing intensity, the stationary approximation loses its validity. We check these concepts by studying first the PE spectrum within the semiclassical model(SCM) for multiple-step pulses. The SCM offers the possibility to establish a connection between emission times and the PE spectrum in the energy domain.We show that PE substructures stem from ionization at different times mapping the pulse envelope. We also analyze the PE spectrum for a realistic sine-squared envelope within the Coulomb-Volkov and ab initio calculations solving the time-dependent Schrödinger equation. We found that the electron emission amplitudes produced at different times interfere with each other producing, in this way, a new additional pattern that modulates the above-threshold ionization (ATI) peaks.","palabras_clave":"PONDEROMOTIVE|LASER|PHOTOIONIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5471,"idioma_id":4,"titulo":"Red-crested Cardinals use color and width as cues to reject Shiny Cowbird eggs","resumen":"As part of the coevolutionary process between brood parasites and their hosts, the latter have developed different strategies to discriminate and reject parasitic eggs. This recognition?rejection process is the primary host defense against costly brood parasitism. The Red-crested Cardinal (Paroaria coronata) is an occasional host of the generalist Shiny Cowbird (Molothrus bonariensis) that successfully rejects all parasitic eggs. We studied the cues used by Redcrested Cardinals to recognize and reject foreign eggs by experimentally adding real parasite and host eggs painted as mimetic or nonmimetic of host eggs and analyzing whether eggshell coloration and\/or shape were used as cues for egg rejection. Rejection rates, mostly through egg ejection, were high for all nonmimetic eggs (95% for unpainted cowbird eggs and 100% for painted nonmimetic cowbird and host eggs). On the contrary, they were low for mimetic host eggs (6% for unpainted host eggs and 20% for painted mimetic host eggs), but intermediate for painted mimetic cowbird eggs (55%). We also found that egg width significantly affected the probability of rejection, with wider parasitic eggs (i.e. more different from host eggs) more frequently rejected. We report for the first time that egg width is an important cue for recognition and ejection of cowbird eggs in an open-cup-nesting host. Our results show that coloration is a reliable cue used by Red-crested Cardinals to discriminate and reject parasitic eggs, but when coloration alone does not allow discrimination of foreign eggs, this host uses egg width as an additional cue.","palabras_clave":"antiparasitic defenses|brood parasitism|egg rejection|Molothrus bonariensis|Paroaria coronata|visual cues"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5472,"idioma_id":4,"titulo":"Density functional studies of small Au clusters adsorbed on &alpha;-FeOOH: Structural and electronic properties","resumen":"We report a density functional theory (DFT) investigation on the interaction of tiny Aun (n = 1-5) clusters with the bare and hydroxylated (110) surfaces of goethite (alpha-FeOOH). Both adsorption and atom-by-atom nucleation processes were modeled. The adsorption is shown to be strong on the bare surface and takes place preferentially through the interaction of Au atoms with unsaturated surface oxygen anions, accompanied with an electronic charge transfer from the metal to the support. Au3, Au4 and Au5 planar structures resulted to be particularly stable due to polarization effects; indeed, Coulombic repulsion between basal Au atoms and surface oxygen anions promotes the displacement of the electronic density toward terminal Au atoms producing a Au+(basal)\/Au-(terminal) polarization. On the hydroxylated surface, Au clusters adsorb more weakly with respect to the bare surface, mainly through monocoordinated surface hydroxyl groups and tricoordinated oxygen ions. Concerning the nucleation mechanism, while on the hydroxylated surface the nucleation energy is governed by the spin of the interacting systems, on the bare surface polarization effects seems to play a predominant role.","palabras_clave":"ADSORPTION|DENSITY FUNCTIONAL CALCULATIONS|GOETHITE|POLARIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5473,"idioma_id":4,"titulo":"Acetylation of bacterial cellulose catalyzed by citric acid: use of reaction conditions for tailoring the esterification extent","resumen":"Bacterial cellulose (BC) nanoribbons were partially acetylated by a simple direct solvent-free route cat-alyzed by citric acid. The assay of reaction conditions within chosen intervals (i.e. esterification time(0.5?7 h), catalyst content (0.08?1.01 mmol\/mmol AGU), and temperature (90?140&#9702;C)), illustrated theflexibility of the methodology proposed, with reaction variables which can be conveniently manipulatedto acetylate BC to the required degree of substitution (DS) within the 0.20?0.73 interval. Within this DSinterval, characterization results indicated a surface-only process in which acetylated bacterial cellulosewith tunable DS, preserved fibrous structure and increased hydrophobicity could be easily obtained. Thefeasibility of reusing the catalyst\/excess acylant in view of potential scale-up was also illustrated.","palabras_clave":"BACTERIAL CELLULOSE|ACETYLATION|CITRIC ACID|REACTION CONDITIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5474,"idioma_id":4,"titulo":"A galectin-specific signature  in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders","resumen":"Inflammatory bowel diseases (IBD) are idiopathic, chronic and relapsing inflammatory conditions of the gastrointestinal tract. Galectins, a family of animal lectins defined by shared consensus amino acid sequence and affinity for &#946;-galactosides containing oligosaccharides, are polyfunctional proteins in a wide range of biological processes, and its role in IBD has not been completely explored. In this work we analyzed the expression of Galectin (Gal)-1, Gal-3, Gal-4 and Gal-9 in intestinal biopsies, and proposed an integrated analysis of the expression of these four galectins as novel mucosal markers for IBD. Colonic biopsies were obtained in 73 IBD patients (24 Crohn´s disease-CD, and 49 ulcerative colitis-UC), 9 patients with rejection after gut transplantation, 8 adult patients with celiac disease and 32 non-IBD donors. The study of galectin expression was performed by mRNA isolation, RT-PCR and qPCR using specific primers for individual galectins and &#946;-actin. A linear discriminant analysis (LDA) was used to analyze the expression of the four galectins in individual biological samples.We found that galectin expression was dysregulated and showed a specific profile in inflamed tissues from IBD, whereas non-inflamed IBD or control samples shared the same pattern expression. LDA could discern between different inflammation grades in active areas of IBD, and showed that remission IBD samples were clusterized with control samples. CD was not distinguished from UC, even with the addition of transcription factors that have been associated to IBD. Furthermore, inflamed IBD was discriminated from inflamed tissue of rejected gut in transplanted patients and duodenum of celiac patients, which were not discriminated from control duodenum samples.","palabras_clave":"IBD|intestinal inflammation|galectins|gene expression"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5475,"idioma_id":4,"titulo":"Relationship Between the Level of Bovine Leukemia Virus Antibody and Provirus in Blood and Milk of Cows from a Naturally Infected Herd","resumen":"We explored the relationship between the level of bovine leukemia virus antibodies and provirus load during natural infection. For that purpose, a set of 50 blood and milk paired samples were analyzed for the presence of bovine leukemia virus provirus and antibodies. Additionally, provirus load and antibody titers were measured and the relationship between these variables was investigated. Bovine leukemia provirus was detected in 59% of milk samples and a negative correlation was observed between the level of milk provirus load and milk antibody titers. By the consumption of raw milk, calves might be exposed to bovine leukemia virus favoring the perinatal transmission of this disease.","palabras_clave":"BLV|milk|colostrum|infectivity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5476,"idioma_id":4,"titulo":"Effect of sucrose concentration on the composition of enzymatically synthesized short-chain fructo-oligosaccharides as determined by FTIR and multivariate analysis","resumen":"Fructo-oligosaccharides (FOS) are mixtures of oligosaccharides composed of fructose and glucose units. As their composition is determined by the synthesis conditions, the goals of this work were: (a) to engineer FOS of different composition by adjusting the sucrose concentration used as initial substrate; (b) to define partial least square (PLS) based-models to quantify all the sugars present in the reaction medium directly from the FTIR spectra. The yield of each reaction was calculated as the percentage of initial sucrose converted to each oligosaccharide, as monitored by HPLC. In parallel, the reactions were followed by FTIR. Six different PLS models aiming to determine the concentration of each carbohydrate present in the reaction medium were calibrated and independently validated. The means of predicted values fitted well to those obtained by HPLC. Determining FOS composition directly from the FTIR spectra represents a useful tool to monitor enzymatic synthesis, with strong impact at both an academic and an industrial level.","palabras_clave":"FOS|FTIR|PLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5477,"idioma_id":5,"titulo":"EL ROL DEL ESTADO FRENTE A LOS DERECHOS SOCIALES PARA LOS ADULTOS MAYORES","resumen":"Abordamosen este trabajo un análisis del rol del Estado frente a la desigualdad socialen una sociedad de y en riesgo, en particular respecto de los adultos mayores.Desigualdad que no es resultado de una única causa sino que es consecuencia dela convergencia de diversos factores: culturales, sociales, económicos,etarios, políticos... Bajo este enfoque y desde la perspectiva de la protecciónsocial, indagamos respecto del desafío de integrar a sectores vulnerados,avanzando hacia una reformulación de las funciones de los diferentes niveles degobierno; poniendo énfasis en la función que le corresponde al Estado, comoactor encargado de brindar servicios sociales y eliminar progresivamente laexistencia de ciudadanías diferenciales. Es decir, avanzar hacia laconceptualización de la protección social, desde un enfoque de derechos deciudadanía.Finalmente, y como parte inescindibledel reconocimiento de esos derechos de todo adulto mayor, nos abocamos alanálisis del acceso a la justicia bajo los lineamientos de la Corte Suprema deJusticia de la Naciónque ha entendido que el derecho consagrado en elartículo 18 de la Constitución Nacional requiere de una efectiva tutelajudicial que resulte oportuna y posea la virtualidad de resolverdefinitivamente la cuestión sometida a su conocimiento.","palabras_clave":"DERECHOS SOCIALES|CIUDADANÍA|ADULTOS MAYORES|PROTECCION SOCIAL-ACCESO A LA JUSTICIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5478,"idioma_id":4,"titulo":"In vitro antagonistic activity of native bacteria isolated from soils of the argentine Pampas against Fusarium tucumaniae and Fusarium virguliforme","resumen":"The aim of the present study was to evaluate indigenous PGPR (Plant growth promoting rhizobacteria) previously isolated from Argentina´s soybean fields for their in vitro antagonistic effects on the control of Fusarium tucumaniae and F. virguliforme, in two separated in vitro assays. In assay 1, the bacteria that showed the highest  significant (P <  0.05) F.  tucumaniae mycelial growth inhibition  were  strains Bacillus subtilis 54 (70%), B. cereus 13 (44%), B. cereus 7 (44%) and Chryseobacterium vietnamense 110 (42%). Despite their antagonistic activity, the strains identified as Stenotrophomonas malthophilia and B. cereus were not included in any further experiments, because of their potential hazard. In assay 2, strains 54, 110 and Pseudomonas fluorescens 9 and 115 were tested against F. tucumaniae and F. virguliforme. In this study, native bacterial strains isolated from Argentine Pampas were tested for the first time against these pathogens. All four bacterial strains significantly inhibited mycelial growth of F. virguliforme. Further studies on the effects of these strains on the growth of soybean plants and on the Sudden Death Syndrome (SDS) control will uncover the mechanisms and in vitro antagonism potential of these bacterial isolates.","palabras_clave":"Antagonism|Fusarium|Plant growth promoting rhizobacteria (PGPR)|Bacillus|Pseudomonas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5479,"idioma_id":5,"titulo":"RELACIONES PALEOFLORISTICAS DEL MIOCENO-PLIOCENO DEL NORTE ARGENTINO","resumen":"Los objetivos de este trabajo son los de relacionar las paleofloras del Mioceno-Plioceno del Norte de la Argentina que caracterizan a cada Formación y a cada período, comparar las halladas en el noroeste con las del noreste a  fin de establecer sus diferencias o semejanzas e inferir la evolución florística y paleoambiental a través del intervalo estratigráfico considerado. Las formaciones seleccionadas son las de Paraná e Ituzaingó en el noreste y las de Anta, San José, Chiquimil, Palo Pintado, Aloformación Playa del Zorro, Andalhuala, del Buey y pozo YPF.SE.x-2 Los Horcones en el noroeste. Se reconocieron 430 especies fósiles que  fueron volcadas como presencia-ausencia en una base de datos en la que se consignaron para cada uno de ellos, la familia, formaciones, tipo de fósil, hábito, comunidad vegetal y representante actual más cercano al fósil. A la misma se aplicó el análisis de agrupamiento simultáneo en modo Q para las formaciones y en modo R para las especies, utilizando la medida de Distancia de Sorensen. Las paleofloras analizadas comparten el 15% del total de las especies relevadas. El análisis integral comparativo permitió señalar una marcada diferenciación paleovegetacional y paleoambiental entre ambas regiones. En el noroeste, desde el Mioceno temprano al Plioceno se registraron estepas graminosas, bosques ribereños y bosques secos con estacionalidad y sabanas herbáceas, es decir una manifiesta alternancia de cambios ambientales y vegetacionales relacionados al clima. Mientras que en el noreste, prevalecen los bosques ribereños y bosques secos con estacionalidad de moderados cambios ambientales.","palabras_clave":"PALEOVEGETACION|NEÓGENA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5480,"idioma_id":4,"titulo":"A lattice discrete element method to model the falling-weight impact test of PMMA specimens","resumen":"It is introduced in this paper a LatticeDiscrete Element Method (LDEM) for modelling the fallingweighttest of polymethyl-methacrylate (PMMA)specimens. The method exploits the inherent characteristicsof discrete methods to model crack initiationand propagation by simply breaking the links between theirdiscrete components. It results in a flexiblemodelling tool that is implemented using Abaqus\/Explicit.Numerical results are validated by comparisonwith experimental tests. The results are compared in termsof the time evolution of the striker forceand velocity and the specimen crack patterns. The LDEM simulationsare, in every case, of predictive nature.Material properties are neither left open for calibrationnor used to adjust the numerical results.There is a good agreement between experimental and numericalresults. It is shown that the proposed LDEMhas the capability to capture all the main features ofthe sequence of events that occur during theexperiment: the elastic specimen loading prior to the crackinitiation, the nucleation and propagation ofradial cracks as the test progresses, and the final failure afterthe rapid propagation of a circular crackthat joins the radial cracks together. The effects of the variabilityof the material fracture toughness on thetest results are studied using a series of models with randomdistribution of the fracture energy.","palabras_clave":"IMPACT TEST|IMPACT OF POLYMERS|LATTICE DISCRETE ELEMENT METHOD|ABAQUS|EXPLICIT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5481,"idioma_id":4,"titulo":"Relationships among invertebrate communities and groundwater properties in an unconfined aquifer in Argentine","resumen":"The paper evaluates the relationships among invertebrate communities, land uses and chemical and microbiological groundwater properties, in a loessic unconfined aquifer in Argentina. Two surveys were conducted and seven wells were selected based on land use and unsaturated zone (USZ) thickness. Groundwater was characterized mainly as freshwater of sodium bicarbonate type. Invertebrates collected belong to Crustacea, Acari, Insecta, Collembola, Oligochaeta, Collembola, Pauropoda, and Nematoda. Crustacea was the most abundant group. The wells differed in terms of invertebrate composition and abundance. The lowest abundance was observed at sites with thickest USZ. No direct relationship was found between invertebrate abundance and any particular physicochemical parameter. In the only well where bacteria were detected, total invertebrate abundance, especially of copepods, was high in both surveys. Both USZ thickness and land use have a significant influence on abundance and composition of invertebrate communities in terms of organic matter inputs into the aquifer","palabras_clave":"Groundwater| Ecosystem| Crustacea|Land-use"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5482,"idioma_id":5,"titulo":"DERMATITIS CERCARIAL EN BAÑISTAS DEL NORTE DE LA PATAGONIA ARGENTINA","resumen":"La dermatitis cercarial es una enfermedad cutánea, zoonótica y estival, causada por una larva nadadorade trematodes de la familia Schistosomatidae, llamada cercaria. Esta larva penetra accidentalmentela piel de personas que se encuentran en el agua, generando una reacción alérgica que generalmente nose informa en centros de salud, dificultando el registro de su incidencia. Se han informado casos en todo el mundo,incluyendo a la Argentina, aunque los estudios epidemiológicos se han realizado solamente en el HemisferioNorte. En este trabajo se estudió la epidemiología de la dermatitis cercarial en tres balnearios patagónicos de loslagos Pellegrini, Mari Menuco y Puelo, donde se realizaron encuestas a visitantes durante los veranos de 2012-2013 y 2013-2014. Se analizaron los factores de riesgo, que fueron agrupados en ?características personales?,?uso del recurso? y ?medidas de prevención?. En el lago Pellegrini fue la sensibilización de la persona incluidaen 'características personales' el único factor determinante para la ocurrencia de esta zoonosis, mientras queen el lago Mari Menuco la ocurrencia se asoció con el tiempo y la distancia a la costa y el uso de cremas. Enel lago Puelo no se registraron casos durante el período de estudio.","palabras_clave":"DERMATITIS CERCARIAL|SCHISTOSOMATIDAE|EPIDEMIOLOGIA|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5484,"idioma_id":4,"titulo":"Cytotoxic effects induced by interferon-w gene lipofection through ROS generation and mitochondrial membrane potential disruption in feline mammary carcinoma cells","resumen":"Progress in comparative oncology promises advances in clinical cancer treatments for both companionanimals and humans. In this context, feline mammary carcinoma (FMC) cells have been proposed as asuitable model to study human breast cancer. Based on our previous data about the advantages of usingtype I interferon gene therapy over the respective recombinant DNA derived protein, the present workexplored the effects of feline interferon-x gene (fIFNx) transfer on FMC cells. Three different cell variantsderived from a single spontaneous highly aggressive FMC tumor were successfully established and characterized.Lipofection of the fIFNx gene displayed a significant cytotoxic effect on the three cell variants.The extent of the response was proportional to ROS generation, mitochondrial membrane potential disruptionand calcium uptake. Moreover, a lower sensitivity to the treatment correlated with a highermalignant phenotype. Our results suggest that fIFNx lipofection could offer an alternative approach inveterinary oncology with equal or superior outcome and with less adverse effects than recombinantfIFNx therapy.","palabras_clave":"FELINE MAMMARY CARCINOMA|INTERFERON-OMEGA|PLASMID LIPOFECTION|ROS|GENE THERAPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5485,"idioma_id":5,"titulo":"Fecha de siembra de maíz en Cañada de Gómez y su relación con las precipitaciones","resumen":"El objetivo del trabajo fue evaluar cómo la distribución de las precipitaciones (PP) en la localidad de Cañada de Gómez incidió en la fecha de siembra (FS) y en el rendimiento (R) del cultivo de maíz.","palabras_clave":"FECHA DE SIEMBRA|RENDIMIENTO|DISTRIBUCIÓN DE PRECIPITACIONES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5486,"idioma_id":5,"titulo":"Cambio climático, breve historia y tendencias en la Región Húmeda","resumen":"El estudio del clima es un campo de investigación complejo y en rápida evolución. Debido a la gran cantidad de factores que intervienen, el clima de la Tierra nunca ha sido estático. Como consecuencia de alteraciones en el balance energético, el clima está sometido a variaciones en todas las escalas temporales, desde decenios a miles y millones de años (Ministerio de Agricultura y Alimentación de Medio Ambiente de España, 2015).El Grupo Intergubernamental de Expertos sobre Cambio Climático (IPCC) define al Cambio Climático como ?Importante variación estadística en el estado medio del clima o en su variabilidad, que persiste durante un período prolongado (normalmente decenios o incluso más). El Cambio Climático se puede deber a procesos naturales internos o a cambios del forzamiento externo, o bien a cambios persistentes antropogénicos en la composición de la atmósfera o en el uso de las tierras?.Se debe tener en cuenta que la Convención Marco de las Naciones Unidas sobre el Cambio Climático (CMCC), define al Cambio Climático como: ?un cambio de clima atribuido directa o indirectamente a la actividad humana que altera la composición de la atmósfera mundial y que se suma a la variabilidad natural del clima observada durante períodos de tiempo comparables?. La CMCC distingue entre ?Cambio Climático? atribuido a actividades humanas que alteran la composición atmosférica y ?variabilidad climática? atribuida a causas naturales.La variabilidad del clima, según el IPCC, se refiere a las variaciones en el estado medio y otros datos estadísticos (como las desviaciones típicas, la ocurrencia de fenómenos extremos, etc.) del clima en todas las escalas temporales y espaciales, más allá de fenómenos meteorológicos determinados. La variabilidad se puede deber a procesos internos naturales dentro del sistema climático (variabilidad interna), o a variaciones en los forzamientos externos antropogénicos (variabilidad externa).","palabras_clave":"CAMBIO CLIMÁTICO|TENDENCIAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5487,"idioma_id":4,"titulo":"Linking the hierarchical decision-making process of fruit choice and the phenotypic selection strength on fruit traits by birds","resumen":"AimsAnimals in search of fleshy fruits forage mostly according to the number of available fruits and then select individual fruits based on reward quality or advertised subtle traits. This hierarchical pattern of fruit choice would be translated into patterns of selection strength mediated by frugivores on fruit display traits. Thus, frugivores would exert higher selection pressures on fruit crop size and lower selection pressures on within-plant variation of phenotypic traits (infructescence, fruit and seed size). However, no attempt to link this behavioral mechanism of hierarchical trait selection to natural selection patterns has been made. Therefore, we sought to determine the relationship between the hierarchical decision-making process of fruit choice and patterns of natural selection on fruit traits.MethodsWe recorded bird visits and measured fruit-related traits (fruit crop size,  fruit diameter and seed weight) in a natural population of Psychotria carthagenensis, a bird-dispersed treelet, in a Yungas forest from Argentina. To assess phenotypic selection patterns on fruit display traits, we performed multivariate selection analysis, and to explicitly identify a hierarchy of fruit trait choice we used a classification tree as a predictive model.Important FindingsSelection patterns on fruit display traits were in agreement with a hierarchical process of fruit choice made by birds. The strength of directional selection on the total number of fruits in a plant (i.e. fruit crop size) was nearly two times higher than on fruit size, and the classification tree analysis supported this hierarchical pattern. Our results support previous evidence that seed dispersers shape fruit crop size with higher intensity than subindividual fruit traits. Also, high  levels  of  subindividual  phenotypic  variation  of  fruit  display traits may be explained by relaxed selection pressures exerted by frugivores. Empirical studies also show that this pattern may constitute a general phenomenon among other plant?animal interactions.","palabras_clave":"PLANT-ANIMAL INTERACTIONS|SEED DISPERSAL|SELECTION GRADIENTS|SUBINDIVIDUAL VARIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5488,"idioma_id":4,"titulo":"Biochemical and microstructural assessment of minimally processed peaches subjected to high pressure processing: implications on the freshness condition","resumen":"Since the condition of freshness is closely linked to the definition of minimally processed fruits, it is generally assumed that they should be constituted by living tissues. High-pressure processing (HPP) has been proven to be successful in maintaining the freshness of foods, although the integrity of some tissues could be altered. The aim of this work was to assess different biochemical and microstructural changes experimented by the HPP-treated tissues. The following determinations were conducted in control and treated diced peaches: viability assay, microstructure analysis, relative expression of RNAm, and enzyme activities related to browning and anaerobic metabolism. Results showed that although HPP-treated tissues were not viable, changes in the expression of the RNAs of the enzymes evidenced that some metabolic processes were still active. In turn, the microstructure remained rather unaltered, while the enzymes tested were significantly inhibited. Then, although some characteristics of living tissues were modified in the HPP-treated fruits, they could be considered as fresh in appearance. Industrial relevance It is well known that conventional thermal treatments applied to preserve fruit products usually cause important changes in flavor, color, texture, and consequently the loss of fresh appearance. However, in the case of novel technologies such as high-pressure processing (HPP), this causality is not straightforward. Interestingly, the claim of ?freshness? in foods has become a controversial topic, since some regulations indicate that for a food to be regarded as fresh, it should not have been subjected to any preservation processing. However, a list of exceptions has been considered for processes such as irradiation at low doses, or application of edible coatings. This list of exception is expected to increase, accordingly to the raise in successful experiences with the application of novel technologies such as HPP. This work provides information on the effect of HPP on different biochemical and structural aspects of treated peaches, with the aim to contribute toward the commercial application of HPP to minimally processed fruits.","palabras_clave":"Freshness|High pressure processing|Microstructure|Peaches|Viability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5489,"idioma_id":4,"titulo":"Social Learning in Vespula Germanica Wasps: Do They Use Collective Foraging Strategies?","resumen":"Vespula germanica is a social wasp that has become established outside its native range in many regions of the world, becoming a major pest in the invaded areas. In the present work we analyze social communication processes used by V. germanica when exploiting un-depleted food sources. For this purpose, we investigated the arrival pattern of wasps at a protein bait and evaluated whether a forager recruited conspecifics in three different situations: foragers were able to return to the nest (full communication), foragers were removed on arrival (communication impeded), or only one forager was allowed to return to the nest (local enhancement restricted). Results demonstrated the existence of recruitment in V. germanica, given that very different patterns of wasp arrivals and a higher frequency of wasp visits to the resource were observed when communication flow between experienced and naive foragers was allowed. Our findings showed that recruitment takes place at a distance from the food source, in addition to local enhancement. When both local enhancement and distant recruitment were occurring simultaneously, the pattern of wasp arrival was exponential. When recruitment occurred only distant from the feeder, the arrival pattern was linear, but the number of wasps arriving was twice as many as when neither communication nor local enhancement was allowed. Moreover, when return to the nest was impeded, wasp arrival at the bait was regular and constant, indicating that naive wasps forage individually and are not spatially aggregated. In conclusion, this is the first study to demonstrate recruitment in V. germanica at a distance from the food source by modelling wasps? arrival to a protein-based resource. In addition, the existence of correlations when communication was allowed and reflected in tandem arrivals indicates that we were not in the presence of random processes.","palabras_clave":"social communication|learning|behavior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5490,"idioma_id":4,"titulo":"RWEQ - Wind erosion predictions for variable soil roughness conditions","resumen":"The soil surface roughness is a main factor in all wind erosion prediction models, including the Revised Wind Erosion Equation (RWEQ). The objective of this study was to test the erodibility of two typical soils of the semiarid Argentinean Pampas under three different tillage conditions (compared to a flat surface) at three wind velocities using a wind tunnel and to evaluate the performance of the RWEQ model. Results showed that all rough surfaces were less eroded by wind than a flat surface (FS) in both soils and all wind velocities. An exception was LB (lister-bedder) in the Haplustoll that showed similar erosion than FS. Wind erosion increased rapidly above 16.5 m s&#8722;1 wind velocity in all tillage conditions. The relative wind erosion (RE) calculated with the RWEQ (K&#8242; factor) fitted well with measured RE, except for K&#8242; < 0.1 (rougher surface) where the measured RE were much higher than the predicted. More than 70% of RE variability was explained by the oriented roughness (Kr) in both soils. The aforementioned indicates that Kr can be used instead of K&#8242; (a value that contains both, Kr and the random roughness ? Crr factors) to predict wind erosion with RWEQ in the studied soils. Absolute wind erosion amounts predicted with RWEQ fitted well with measured data only for DT, mainly at low wind velocity. For the other tillage tools, the model did not apply well as it underestimated the erosion for the rougher soil surface condition (LB) and overestimated it for the less rough surface (DH).","palabras_clave":"SOIL SURFACE ROUGHNESS|K' FACTOR|WIND EROSION|RWEQ MODEL|WIND TUNNEL SIMULATIONS|LOESS SOILS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5491,"idioma_id":5,"titulo":"Expertos, burocracias y política de masas en Argentina","resumen":"Introducción al dossier que reúne artículos de Ricardo Salvatore, Ignacio Allevi y Andrés Stagnaro con el propósito de refl exionar sobre la renovación en los estudios de la historia del Estado y, en particular, explorar la cuestión del vínculo entre los expertos, las burocracias y la política de masas en el amplio y turbulento período que media desde la consolidación de la Argentina moderna hasta el peronismo clásico.El trabajo realiza un recorrido por los principales aportes de la historia del Estado de los últimos años, las contribuciones de los autores del dossier y plantea nuevos interrogantes y perspectivas que ayudan a profundizar nuestro conocimiento sobre estas problemáticas.","palabras_clave":"ESTADO|BUROCRACIAS|POLITICA DE MASAS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5493,"idioma_id":4,"titulo":"Development of polymer-supported chiral aminoalcohols derived from biomass and their application to asymmetric alkylation","resumen":"A synthetic strategy has been developed for the preparation of immobilized 1,2-aminoalcohols starting from easily available and renewable chiral building blocks. They were tested as chiral ligands for the asymmetric diethylzinc addition to carbonyl compounds. Enantioselectivities were comparable to those observed for non-immobilized analogs. These results provide strong evidence for the flexibility of our approach to generate highly valuable supported chiral ligands derived from cellulose-rich materials","palabras_clave":"RECICLADO|BIOMASA|LEVOGLUCOSENONA|CATALIZADORES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5494,"idioma_id":4,"titulo":"Arsenic adsorption onto aluminium-substituted goethite","resumen":"Aluminium and iron oxy(hydr)oxides in nature are often partially substituted by the other elements and strongly influence the mobility of arsenic in the environment. Since Because goethite is commonly found in soils, and the oxide is easily substituted, in the presentis work, the adsorption of AsV onto several Al-substituted goethites was explored in order to determine how the substitution affects the adsorption process. Three samples with increasing Al-content (GAl0, GAl3.78 and GAl7.61) were prepared and fully characterised. The variations in AsV adsorption at under different conditions, as well as the variations of the particle surface charge (ZP), were also analysed. The results showed that the removal capacity of Al-goethites is determined by the Al-content. The adsorption maxima per gram followed the trend GAl0> GAl3.78> GAl7.61, indicating that Al-incorporation decreases AsV sorption. The Adsorption per surface area decreased in the order GAl3.78> GAl0> GAl7.61, implying that a small incorporations of Al enhances the adsorption properties of the surface. The stoichiometry of the probable surface complexes formed with the contaminant at different pH values is proposed, by means of the analysis of all the experimental results obtained before and after AsV adsorption. These surface complexes were used to fit the experimental data with a good agreement, and the formation and acidity c constants were also estimated.","palabras_clave":"AL-GOETHITES|ZETA POTENTIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5495,"idioma_id":4,"titulo":"Demographic history inferred from genome-wide data reveals two lineages of sheldgeese endemic to a glacial refugium in the southern Atlantic","resumen":"Aim The Malvinas\/Falkland Islands (MFI) constitute the largest archipelago in the southern Atlantic, and harbour endemic lineages that presumably evolved after sea-level rise, associated with glacial periods, isolated ancestral populations. We investigate the role of the MFI in isolating populations from continental counterparts of two highly vagile species: the sheldgeese Chloephaga picta and Chloephaga rubidiceps.Location Patagonia and the Malvinas\/Falkland Islands.Methods We sampled C. picta and C. rubidiceps on the continent and MFI. Using a reduced-representation genomic approach, we quantified the genetic differentiation between insular and continental populations of both species, and used coalescent-based analyses to model their demography.Results The MFI harbour independently evolving lineages of C. picta and C. rubidiceps, which diverged from their continental counterparts during the Middle-Late Pleistocene and have since experienced negligible gene flow.Main conclusions The c. 450 km that separate the archipelago from the continent are sufficient to isolate populations of these putatively highly vagile species. Ancestral lineages may have reached the MFI refugium during glacial cycles. Without conservation measures, the drastic decline of the morphologically, behaviourally and ecologically distinct continental population of C. rubidiceps, to < 1000 individuals, may lead to the extinction of an independently evolving taxon.","palabras_clave":"Chloephaga|Conservation genetics|demographic modelling|endangered species|island endemism"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5497,"idioma_id":4,"titulo":"A TRANSIENT FAULT-VALVE MECHANISM OPERATING IN UPPER CRUSTAL LEVEL, SIERRAS PAMPEANAS, ARGENTINA","resumen":"Located in the Sierras Pampeanas (the broken-foreland of the Pampean flat slab segment in the southern Central Andes), the Cerro Tiporco volcanic field shows Neogene hydrothermal activity linked to migration of arc-magmatism into the foreland. Late Neogene deposits comprise epithermal vein systems emplaced in Precambrian&mdash;Early Palaeozoic igneous-metamorphic basement, Late Miocene sedimentary rocks and Early Pliocene volcaniclastic rocks. Mineralization consists of calcareous onyx, aragonite and calcite veins as well as travertine deposits. Onyx and aragonite occur as fill of low-displacement nearly vertical reverse-sinistral faults striking NW, and nearly horizontal dilatant fractures. The latter consist of load-removal induced fractures affecting the igneous-metamorphic rocks, as well as bedding planes in the Late Miocene sediments. The presence of veins recording multiple fracture episodes and crack-and-seal growth of veins suggests relatively low differential stress and supralithostatic fluid pressure, as well as cyclic changes in pore pressure and high mineral-deposition\/fracture-opening ratio. These conditions support a mechanism of fault-valve behaviour during onyx and aragonite vein emplacement. The fault-valve mechanism involves fractures associated with impermeable barriers between environments with different fluid pressure. Faulting generated an appreciable directional permeability triggering fluid migration from the highest to the lowest pressure region, with subsequent deposition and sealing that started a new pressurization-faulting-sealing cycle. Late aragonite and calcite veins suggest a change in kinematics indicating the onset of tectonic-load conditions.","palabras_clave":"FAULT-VALVE BEHAVIOUR|CRACK AND SEAL VEIN GROWTH|TERTIARY VOLCANIC BELT|SIERRAS DE SAN LUIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5498,"idioma_id":4,"titulo":"Bioremediation of heavily hydrocarbon-contaminated drilling wastes by composting","resumen":"Oil-based drilling cuttings comprise a large and hazardous waste stream generated by oil and gas wells drilling operations. Oil-based cuttings are muddy materials with high contents of salts and hydrocarbons. Composting strategies have shown to be effective in the biodegradation of petroleum hydrocarbons, and it offers numerous advantages in comparison with other bioremediation methods. In order to assess the effectiveness of drilling cuttings bioremediation by composting with food and garden wastes, an experiment was conducted in 60-L reactors for 151 days. Four treatments were carried out: only oil-based cuttings, two proportions (in a volume basis) of organic wastes to drilling cuttings (33 and 75 %) and only organic wastes (as a traditional composting reference), with pine-tree woodchips as bulking agent. High degradation percentages of total hydrocarbons (&#8776;82 %), n-alkanes (&#8776;96 %) and the 16 USEPA-listed polycyclic aromatic hydrocarbons (&#8776;93 %) were reached in the treatment with 75 % of organic wastes, and applying 33 % of organic wastes was not more effective than not applying organic wastes for the drilling cuttings hydrocarbons biodegradation. Furthermore, in the treatment with 75 % of organic wastes, alkanes half-life and polycyclic aromatic hydrocarbons half-life were about 10 times and four times lower, respectively, than those in the treatment with 33 % of organic wastes. Possibly, lower hydrocarbons and salts initial concentrations (i.e., lower toxicity), higher microbial counts, adequate nutrient proportions and water content supported a high biological activity with a consequent elevated biodegradation rate in the treatment with 75 % of organic wastes.","palabras_clave":"Drilling wastes treatment|Hydrocarbons biodegradation|Oil-based drilling cuttings|Organic Wastes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5499,"idioma_id":4,"titulo":"Photodegradation alleviates the lignin bottleneck for carbon turnover in terrestrial ecosystems","resumen":"A mechanistic understanding of the controls on carbon storage andlosses is essential for our capacity to predict and mitigate humanimpacts on the global carbon cycle. Plant litter decomposition is animportant first step for carbon and nutrient turnover, and litter inputsand losses are essential in determining soil organic matter pools andthe carbon balance in terrestrial ecosystems. Photodegradation, thephotochemical mineralization of organic matter, has been recentlyidentified as a mechanism for previously unexplained high rates oflitter mass loss in arid lands; however, the global significance of thisprocess as a control on carbon cycling in terrestrial ecosystems is notknown. Here we show that, across a wide range of plant species,photodegradation enhanced subsequent biotic degradation of leaflitter. Moreover, we demonstrate that the mechanism for thisenhancement involves increased accessibility to plant litter carbohydratesfor microbial enzymes. Photodegradation of plant litter, drivenby UV radiation, and especially visible (blue?green) light, reduced thestructural and chemical bottleneck imposed by lignin in secondary cellwalls. In leaf litter from woody species, specific interactions with UVradiation obscured facilitative effects of solar radiation on biotic decomposition. The generalized effect of sunlight exposure on subsequentmicrobial activity, mediated by increased accessibility to cellwall polysaccharides, suggests that photodegradation is quantitativelyimportant in determining rates of mass loss, nutrient release,and the carbon balance in a broad range of terrestrial ecosystems.","palabras_clave":"Lignin|Sunlight|Decomposition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5500,"idioma_id":4,"titulo":"Extraction of dynamic speckle activity information from digital holograms","resumen":"In this work we show how dynamic speckle information can be extracted directly from digital holograms. This allows improving the analysis and characterization of dynamic phenomena by combining dynamic speckle with digital holographic interferometry measurements. We have studied the drying process of paint coatings, which is a typical study case in the field of dynamic speckle characterization, since the speckle activity (SA) of drying coatings is known to decay smoothly as a function of time. We recorded both holograms and speckle images during the process. In this way, we could compare the evolution of global SA calculated from speckle images by a standard method with the evolution of speckle correlation extracted directly from the holograms (i.e. without using holographic imagereconstruction algorithms). The results obtained from both methods have shown to be in good agreement.","palabras_clave":"DYNAMIC SPECKLE|SPECKLE CORRELATION|DIGITAL HOLOGRAPHIC INTERFEROMETRY|DYNAMIC PHENOMENA|CHARACTERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5501,"idioma_id":4,"titulo":"In vitro selection of Staphylococcus aureus mutants resistant to tigecycline with intermediate susceptibility to vancomycin","resumen":"Background: Tigecycline (TIG) is an antibiotic belonging to the glycylcyclines class and appears to be a good choice to fight infections caused by Staphylococcus aureus. To date, TIG exhibits good activity against this microorganism.The aim of this work was to obtain in vitro mutants of S. aureus resistant to TIG and evaluate possible changes in their susceptibility patterns to other antibiotics.Results: Two mutants of S. aureus resistant to TIG (MIC = 16 µg\/mL) were  selected in vitro from clinical isolates of methicillin-resistant S. aureus. In both mutants, corresponding to different lineage (ST5 and ST239), an increase ofefflux activity against TIG was detected. One mutant also showed a reduced susceptibility to vancomycin, corresponding to the VISA phenotype (MIC = 4 µg\/mL), with a loss of functionality of the agr locus. The emergence of theVISA phenotype was accompanied by an increase in oxacillin and cefoxitin MICs.Conclusions: This study demonstrates that, under selective pressure, the increase of efflux activity in S. aureus is one of the mechanisms that may be involved in the emergence of tigecycline resistance. The emergence of this phenotype may eventually be associated to changes in susceptibility.","palabras_clave":"STAPHYLOCOCCUS AUREUS|TIGECICLINA|VANCOMICINA|EFLUJO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5502,"idioma_id":4,"titulo":".    Increase in IS256 transposition in invasive vancomycin heteroresistant        Staphylococcus aureus isolate belonging to ST100 and its derived VISA mutants","resumen":"In Staphylococcus aureus, transposition of IS256 has been described to play an important role in biofilm formation and antibiotic resistance. This study describes the molecular characterization of two clinical heterogeneous vancomycin-intermediate S. aureus (hVISA) isolates recovered from the same patient (before and after antibiotic treatment) and two VISA derivatives obtained by serial passages in the presence of vancomycin. Our results showed that antibiotic treatment (in vivo and in vitro) could enhance IS256 transposition, being responsible for the eventual loss of agr function. As far as we know this is the first study that reports the increase of IS256 transposition in isogenic strains after antibiotic treatment in a clinical setting.","palabras_clave":"S. aureus|IS256 transposition|hVISA\/VISA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5503,"idioma_id":4,"titulo":"Design of chitosan-based nanoparticles functionalized with gallic acid","resumen":"Active nanoparticles based on chitosan could be applied as a support for the modulation of gallic acid delivery. In this sense, these nanostructures could be employed in different fields such as food, packaging, and pharmaceutical areas. The design parameters of chitosan-based nanoparticles functionalized with gallic acid (GA) were optimized through RSM by means of the analysis of zeta potential (ZP) and percentage encapsulation efficiency (PEE). The nanoparticles were prepared by ionotropic gelation using tripolyphosphate (TPP), at different combinations of chitosan (CH) concentration, CH:TPP ratio and GA. Global desirability methodology allowed finding the optimum formulation that included CH 0.76% (w\/w), CH:TPP ratio of 5 and 37mgGA\/gCH leading to ZP of +50mV and 82% of PEE. Analysis through QuickScan and turbidity demonstrated that the most stable nanoparticle suspensions were achieved combining concentrations of chitosan ranging between 0.5 and 0.75% with CH:TPP ratios higher than 3. These suspensions had high stability confirmed by means ZP and transmittance values which were higher than +25mV and 0.21 on average, respectively, as well as nanoparticle diameters of about 140nm. FTIR revealed the occurrence of both hydrogen bond and ionic interactions of CH-TPP which allowed the encapsulation and the improvement of the stability of the active agent.","palabras_clave":"Central composite design|Zeta potential|Percentage encapsulation efficiency|QuickScan|Ionotropic gelation|RSM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5504,"idioma_id":4,"titulo":"Second-order capillary electrophoresis diode array detector data modeled with the Tucker3 algorithm. A novel strategy for Argentinean white wine discrimination respect to grape variety","resumen":"Data obtained by capillary electrophoresis with diode array detection (CE-DAD) were modeled with the purpose to discriminate Argentinean white wines samples produced from three grape varieties (Torrontés, Chardonnay, and Sauvignon blanc). Thirty eight samples of commercial white wine from four wine-producing provinces of Argentina (Mendoza, San Juan, Salta, and Rio Negro) were analyzed. CE-DAD matrices with dimensions of 421 elution times (from 1.17 to 7.39 minutes) × 71 wavelengths (from 227 to 367 nm) were joined in a three way data array and decomposed by Tucker3 method under non-negativity constraint, employing 18, 18 and 6 factors in the modes 1, 2 and 3, respectively. Using the scores of Tucker model, it was possible to discriminate samples of Argentinean white wine by linear discriminant analysis and Kernel linear discriminant analysis. Core element analysis of the Tucker3 model allows identifying the loading profiles in spectral mode related to Argentinean white wine samples","palabras_clave":"ARGENTINEAN WHITE WINE |CAPILLARY ELECTROPHORESIS |TUCKER3|LDA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5505,"idioma_id":4,"titulo":"Distribution of megafaunal species in the Southwestern Atlantic: key ecological areas and opportunities for marine conservation","resumen":"During the last centuries, populations of marine megafauna?such as seabirds, turtles, andmammals?were intensively exploited. At present, other threats such as bycatch and pollution affect these species, which play key ecological roles in marine ecosystems as apex consumers and\/or nutrient transporters. This study analyses the distribution of six megafaunal species (Chelonia mydas, Caretta caretta, Dermochelys coriacea, Thalassarchemelanophris, Otaria flavescens, and Arctocephalus australis) coexisting in the Southwestern Atlantic to discuss their protection in terms of currentmanagement strategies in the region. Through the prediction of the species potential distributions and their relation to bathymetry, sea temperature and oceanographic fronts, key ecological areas are defined from a multi-taxa perspective. Information on the distribution of 70 individuals (18 sea turtles, 19 albatrosses, and 33 otariids) was obtained through satellite tracking conducted during 2007?2013 and analysed using a Geographic Information System and maximum entropy models. During the autumn?winter period, megafaunal species were distributed over the continental shelves of Argentina, Uruguay, and Brazil, mainly over the Argentine Exclusive Economic Zone and the Argentina-Uruguay Common Fishing Zone. Despite some differences, all megafaunal species seems to have similar environmental requirements during the autumn?winter period. Mostly waters shallower than 50 m were identified as key ecological areas, with the R&#305;´o de la Plata as the habitat with the highest suitability for all the species. This area is highly productive and sustains the main coastal fisheries of Uruguay and Argentina, yet its role as a key ecological area for megafaunal species has been underestimated until now. This approach provides a basis to analyse the effect of anthropic activities on megafaunal species through risk maps and, ultimately, to generate knowledge to improve national and bi-national management plans between Argentina and Uruguay","palabras_clave":"Predadores tope|Distribución oceanica|Conservación y manejo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5507,"idioma_id":4,"titulo":"Is the infectiousness of dogs naturally infected with Trypanosoma cruzi associated with poly-parasitism?","resumen":"Interactions among different species of parasites co-infecting the same host could be synergistic or antagonistic. These interactions may modify both the frequency of infected hosts and their infectiousness, and therefore impact on transmission dynamics.This study determined the infectiousness of Trypanosoma cruzi-seropositive dogs (using xenodiagnosis) and their parasite load (quantified by qPCR), and tested the association between both variables and the presence of concomitant endoparasites. A cross-sectional serosurvey conducted in eight rural villages from Pampa del Indio and neighboring municipalities (northeastern Argentina) detected 32 T. cruzi-seropositive dogs out of 217 individuals examined for infection. Both the infectiousness to the vector Triatoma infestans and parasite load of T. cruzi-seropositive dogs examined were heterogeneous. A statistically significant, nine-fold higher mean infectiousness was registered in T. cruzi-seropositive dogs co-infected with Ancylostoma caninum and a trematode than in T. cruzi-seropositive dogs without these infections. The median parasite load of T. cruzi was also significantly higher in dogs co-infected with these helminths. An opposite trend was observed in T. cruzi-seropositive dogs that were serologically positive to Toxoplasma gondii or Neospora caninum relative to dogs seronegative for these parasites. Using multiple logistic regression analysis with random effects, we found a positive and significant association between the infectiousness of T. cruzi-seropositive dogs and co-infections with A.45 caninum and a trematode. Our results suggest that co-infections may be a modifier of46 host infectiousness in dogs naturally infected with T. cruzi.","palabras_clave":"Trypanosoma cruzi|reservorios animales|Enfermedad de Chagas|infecciosidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5508,"idioma_id":4,"titulo":"Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356","resumen":"In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high salt conditions (0.6M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high salt conditions it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell-wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-Slayer-producing species such as L. casei. Also, the stationary phase and growth in high salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity.","palabras_clave":"S-layer protein|gene expression|osmotic stress|Lactobacillus acidophilus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5509,"idioma_id":4,"titulo":"A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis","resumen":"AbstractBackground: Bovine leukemia virus (BLV) is a member of retroviridae family, together with human T cell leukemia virus types 1 and 2 (HTLV-1 and -2) belonging to the genes deltaretrovirus, and infects cattle worldwide. Previous studes have classified the env sequences of BLV provirus from different geographic locations into eight genetic groups. To investigate the genetic variability of BLV in South America, we performed phylogenetic analyses of whole genome and partial env gp51 sequences of BLV strains isolated from Peru, Paraguay and Bolivia, for which no the molecular characteristics of BLV have previously been published, and discovered a novel BLV genotype, genotype-9, in Bolivia.Results: In Peru and Paraguay, 42.3 % (139\/328) and over 50 % (76\/139) of samples, respectively, were BLV positive. InBolivia, the BLV infection rate was up to 30 % (156\/507) at the individual level. In Argentina, 325\/420 samples were BLV positive, with a BLV prevalence of 77.4 % at the individual level and up to 90.9 % at herd level. By contrast, relatively few BLV positive samples were detected in Chile, with a maximum of 29.1 % BLV infection at the individual level. We performed phylogenetic analyses using two different approaches, maximum likelihood (ML) tree and Bayesian infer&#8209;ence, using 35 distinct partial env gp51 sequences from BLV strains isolated from Peru, Paraguay, and Bolivia, and 74 known BLV strains, representing eight different BLV genotypes from various geographical locations worldwide. Theresults indicated that Peruvian and Paraguayan BLV strains were grouped into genotypes-1, -2, and -6, while those  from Bolivia were clustered into genotypes-1, -2, and -6, and a new genotype, genotype-9. Interestingly, these results were confirmed using ML phylogenetic analysis of whole genome sequences obtained by next generation sequencing of 25 BLV strains, assigned to four different genotypes (genotypes-1, -2, -6, and -9) from Peru, Paraguay, and Bolivia. Comparative analyses of complete genome sequences clearly showed some specific substitutions, in both structural and non-structural BLV genes, distinguishing the novel genotype-9 from known genotypes.Conclusions: Our results demonstrate widespread BLV infection in South American cattle and the existence of a new BLV genotype-9 in Bolivia. We conclude that at least seven BLV genotypes (genotypes-1, -2, -4, -5, -6, -7, and -9) arecirculating in South America.","palabras_clave":"Bovine leukemia virus|Next generation sequencing|BLV whole genome analysis|gp51 env sequencing|Phylogenetic analysis|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5510,"idioma_id":4,"titulo":"Molecular diversi&#64257;cation of Trichuris spp. from Sigmodontinae (Cricetidae) rodents from Argentina based on mitochondrial DNA sequences","resumen":"A molecular phylogenetic hypothesis is presented for the genus Trichuris based on sequence data from mitochondrial cytochrome c oxidase 1 (cox1) and cytochrome b (cob). The taxa consisted of nine populations of whipworm from five species of Sigmodontinae rodents from Argentina. Bayesian Inference, Maximum Parsimony and Maximum Likelihood methods were used to infer phylogenies for each gene separately but also for the combined mitochondrial data and the combined mitochondrial and nuclear dataset. Phylogenetic results based on cox1 and cob mitochondrial DNA (mtDNA) revealed three clades strongly resolved corresponding to three different species (Trichuris navonae, Trichuris bainae and Trichuris pardinasi) showing phylogeographic variation, but relationship among Trichuris species were poorly resolved. Phylogenetic reconstruction based on concatenated sequences had greater phylogenetic resolution for delimiting species and populations intra-specific of Trichuris that those based on partitioned genes. Thus, populations of T. bainae and T. pardinasi could be affected by geographical factors and co-divergence parasite-host.","palabras_clave":"Argentina.|Sigmodontinae. |Trichuri"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5511,"idioma_id":5,"titulo":": ?Políticas migratorias y dictadura militar en Argentina (1976-1983): La construcción de un modelo migratorio?","resumen":"En el presente estudio se analiza el modelo migratorio instalado durante la última dictadura militar en la argentina (1976-1983) con la intención de evidenciar los cimientos a partir de los cuales se ?gestionó? la migración por más de 26 años. El interés se debe a que la disposición y continuidad de esta política pública generó una forma de tratar la migración limítrofe que hoy en día, a pesar del cambio de política dado en el año 2003, sigue repercutiendo en la práctica cotidiana, tanto de los organismos públicos como de la población en general. Para establecer las relaciones propuestas, se trabajó mediante un diseño metodológico cualitativo que combina el análisis socio-jurídico y documental de la legislación y reglamentaciones y el método histórico comparativo","palabras_clave":"Politicas Migratorias|Proyecto de nación |Dictadura|Estado"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5512,"idioma_id":4,"titulo":"An algorithm based on generalized procrustes analysis to find the consensus of several configurations of individuals connected by common checks","resumen":"In field evaluation of large germplasm collections, the experimental material must be divided into manageable experimental trials. In each trial, a set of entries is evaluated by several descriptors. The sets of individuals are different between trials, but an important characteristic of this design is the presence of a certain number of entries involved in all trials, these ones are used as connections between them. The information obtained from these trials is presented by incomplete matrices, because not all individuals are evaluated in all the conditions (trials). Considering this problem, the present algorithm is based on performing a Generalized Procrustes Analysis on the individuals thatare common to all conditions, considering the rest of the individuals ineach condition as supplementary individuals. This algorithm allows to obtain a consensus configuration when the initial matrices are not complete but are connected by a group of individuals in common. The algorithm was illustrated by a simulation of the experimental situation with three conditions and 16 Individuals.","palabras_clave":"GERMPLASM EVALUATION|MULTIWAY MATRICES|SUPPLEMENTARY INDIVIDUALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5513,"idioma_id":4,"titulo":"High frequency of Chlamydia trachomatis mixed infections detected by microarray assay in South American samples","resumen":"AbstractChlamydia trachomatis is one of the most common sexually transmitted infections worldwide. Based on sequence variation in the ompA gene encoding the major outer membrane protein, the genotyping scheme distinguishes 17 recognized genotypes, i.e. A, B, Ba, C, D, Da, E, F, G, H, I, Ia, J, K, L1, L2, and L3. Genotyping is an important tool for epidemiological tracking of C. trachomatis infections, including the revelation of transmission pathways and association with tissue tropism and pathogenicity. Moreover, genotyping can be useful for clinicians to establish the correct treatment when LGV strains are detected. Recently a microarray assay was described that offers several advantages, such as rapidity, ease of standardization and detection of mixed infections. The aim of this study was to evaluate the performance of the DNA microarray-based assay for C. trachomatis genotyping of clinical samples already typed by PCR-RFLP from South America. The agreement between both typing techniques was 90.05% and the overall genotype distribution obtained with both techniques was similar. Detection of mixed-genotype infections was significantly higher using the microarray assay (8.4% of cases) compared to PCR-RFLP (0.5%). Among 178 samples, the microarray assay identified 10 ompA genotypes, i.e. D, Da, E, F, G, H, I, J, K and L2. The most predominant type was genotype E, followed by D and F.  Figures                                                                                                                                                                ","palabras_clave":"Chlamydia trachomatis|Mixed Infections|South American|Microarray Assay"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5514,"idioma_id":4,"titulo":"Comparison of multilocus sequence typing and multilocus typing microarray of Chlamydia trachomatis strains from Argentina and Chile","resumen":"This study compared conventional ompA genotyping of Chlamydia trachomatis with multilocus sequence typing (MLST)and multilocus typing (MLT) DNA microarray.DNA extracts of 104 C. trachomatis positive specimens were analyzed by ompA sequencing and MLST and of these76 by MLT array. Obtained MLST sequence types (STs) were compared to sequences in the database http:\/\/mlstdb.uu.se. The resolution obtained for MLST (35 STs) was 2.1 higher than for ompA sequencing (17 variants) and 1.3 higherthan MLT array (27 MLT groups). Among the 104 samples the predominant genotype E could be divided into 5 ompAvariants and 23 STs of which 16 had not been reported in previous studies. The most common STs, ST3 and ST56, wereidentified as founders and are common in several countries on a global scale. The MLST and the MLT array providedsimilar strain discrimination capacity and showed considerably higher resolution than conventional ompA sequencing.","palabras_clave":"Chlamydia trachomatis|DNA array|genotyping|MLST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5515,"idioma_id":5,"titulo":"Enseñar en la escuela secundaria. Condiciones de trabajo en la Argentina de comienzos del siglo XXI","resumen":"En Argentina, los primeros años del nuevo siglo han sido escenario de experiencias de rebelión popular que plantearon profundos cuestionamientos al consenso neoliberal hegemónico de décadas anteriores. Como estrategia de recomposición de la legitimidad institucional, los gobiernos kirchneristas apelaron a la canalización de algunas expectativas encumbradas en los tiempos de la crisis, pero desarticulando, también, otras que se mostraron tributarias de una perspectiva de radicalización profunda de la sociedad. En el plano de la olítica educacional, el dimensionamiento de los impactos regresivos de las reformas de los 90 operó como el telón de fondo de un nuevo marco de regulaciones. Desde esta caracterización, nos proponemos reconstruir las directrices que el nuevo marco legal nacional le confiere a las políticas destinadas a regular el trabajo de los profesores de educación secundaria, haciendo especial hincapié en el análisis de los cambios introducidos en lascondiciones laborales y en los dispositivos de formación.","palabras_clave":"Educación secundaria|condiciones laborales|trabajo en la escuela secudnaria"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5516,"idioma_id":4,"titulo":"Viewing geometry affects sexual dichormatism and conspicuousness of noniridescent plumage of Swallow Tanagers (Tersina viridis)","resumen":"Some types of plumage color are difficult to characterize spectrophotometrically because the properties of the reflected light change with viewing geometry (i.e. the relative positions of the light source and the observer, and the orientation of the feather). This is the case for the non iridescent plumage coloration of the Swallow Tanager ( Tersina viridis), which seems to change from a human perspective as the angle between the light source and the observer varies. In this study, we measured plumage reflectance with different angles of illumination and\/or observation, and used avian visual models to evaluate the change in sexual dichromatism and conspicuousness with viewing geometry from a bird?s perspective. We also calculated different color parameters to assess how these changed with viewing conditions. Sexual dichromatism showed large changes, with its maximum coinciding with the angle combination between illuminant and observer that produced both the highest conspicuousness for males and the highest crypsis for females. The conspicuousness of males also varied with viewing geometry, and was consistently less when viewed by the visual system of a potential avianpredator (VS) than by that of a conspecific (UVS). The change in perceived coloration was mainly related to large variation in hue and chroma in the plumage of males as the relative angle between the illumination and observation probes changed. Our results show that viewing geometry can alter color perception, even for non iridescent plumage coloration. Therefore, the relative position of the light source and the observer should be considered in studies of avian visual communication, particularly for species with plumage coloration similar to that of Swallow Tanagers.","palabras_clave":"PLUMAGE COLORATION|SEXUAL DICHORMATISM|CONSPICUOUSNESS|VIEWING GEOMETRY|SWALLOW TANAGER|TERSINA VIRIDIS|DOUBLE SCATTERING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5517,"idioma_id":4,"titulo":"First documented record of the Rufous-tailed Attila (Tyrannidae) for Corrientes, Argentina","resumen":"We report on the capture of an adult male Rufous-tailed Attila (Attila phoenicurus) in Mburucuyá National Park, Corrientes, Argentina. This is the first record for this protected area, and also the first record for the province. Records for this species are scarce in Argentina, where it had been registered previously only in Misiones province. Populations of this species are believed tobe declining, and it is considered a ?threatened species? in Argentina. If confirmed, the regular presence and perhaps even breeding of the Rufous-tailed Attila in Mburucuyá National Park would extend the species? distribution range approximately 200 km to the southwest, implying that this threatened species occurs in at least one large protected area of Argentina.","palabras_clave":"Attila phoenicurus|documented record|Mburucuyá National Park"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5518,"idioma_id":4,"titulo":"Energy estimation of cosmic rays with the Engineering Radio Array of the Pierre Auger Observatory","resumen":"The Auger Engineering Radio Array (AERA) is part of the Pierre Auger Observatory and is used todetect the radio emission of cosmic-ray air showers. These observations are compared to the data of thesurface detector stations of the Observatory, which provide well-calibrated information on the cosmic-rayenergies and arrival directions. The response of the radio stations in the 30?80 MHz regime has beenthoroughly calibrated to enable the reconstruction of the incoming electric field. For the latter, the energydeposit per area is determined from the radio pulses at each observer position and is interpolated using atwo-dimensional function that takes into account signal asymmetries due to interference between thegeomagnetic and charge-excess emission components. The spatial integral over the signal distribution givesa direct measurement of the energy transferred from the primary cosmic ray into radio emission in theAERA frequency range. We measure 15.8 MeV of radiation energy for a 1 EeV air shower arrivingperpendicularly to the geomagnetic field. This radiation energy?corrected for geometrical effects?is usedas a cosmic-ray energy estimator. Performing an absolute energy calibration against the surface-detectorinformation, we observe that this radio-energy estimator scales quadratically with the cosmic-ray energy asexpected for coherent emission. We find an energy resolution of the radio reconstruction of 22% for thedata set and 17% for a high-quality subset containing only events with at least five radio stations withsignal.","palabras_clave":"AUGER ENGINEERING RADIO ARRAY|ULTRA HIGH ENERGY COSMIC RAYS|COSMIC RAYS ENERGY CALLIBRATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5519,"idioma_id":4,"titulo":"Measurement of the Radiation Energy in the Radio Signal of Extensive Air Showers as a Universal Estimator of Cosmic-Ray Energy","resumen":"We measure the energy emitted by extensive air showers in the form of radio emission in the frequency range from 30 to 80 MHz. Exploiting the accurate energy scale of the Pierre Auger Observatory, we obtain a radiation energy of 15.8 Æ 0.7ðstatÞ Æ 6.7ðsystÞ MeV for cosmic rays with an energy of 1 EeV arriving perpendicularly to a geomagnetic field of 0.24 G, scaling quadratically with the cosmic-ray energy. A comparison with predictions from state-of-the-art first-principles calculations shows agreement with our measurement. The radiation energy provides direct access to the calorimetric energy in the electromagnetic cascade of extensive air showers. Comparison with our result thus allows the directcalibration of any cosmic-ray radio detector against the well-established energy scale of the Pierre Auger Observatory.","palabras_clave":"Cosmic Rays|Radio Technique"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5520,"idioma_id":4,"titulo":"Fluidized-bed Melt Granulation: Coating and Agglomeration Kinetics and Growth Regime Prediction","resumen":"Mass and population balance equations are used to describe the spray-on melt granulation process in a batch fluidized bedwhere coating and agglomeration can occur simultaneously. Two different models are considered to describe the agglomerationkinetics: the Equipartition Kinetic Energy (EKE) and Size Independent (SI) kernels, while the coating kinetics isrepresented by a power law. Based on experimental data obtained under different operating conditions (melt and air atomizationflowrates, air fluidization velocity, seed diameter and bed temperature), the coating and agglomeration kineticparameters are fitted to correctly predict the final particle size distributions. It is concluded that the SI kernel and the zeroordercoating kinetics best describe the agglomeration and coating mechanisms, respectively. A correlation to predict theSI kernel from macroscopic variables and properties of the system is proposed. This tool can be used to provide startingkernel factors for solving the PBEs governing spray-on melt granulation in fluidized-bed granulators. Finally, a criterionto predict the limit of agglomeration occurrence is formulated. The criterion, which is developed in terms of dimensionlessnumbers that depend on process conditions and take into account mass, heat and momentum transfer phenomena, isfound to be appropriate to predict the growth regime for fluidized-bed spray-on melt granulation of urea (i.e., the specificstudied system).","palabras_clave":"MELT GRANULATION|FLUIDIZED BED|UREA|COATING|AGGLOMERATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5521,"idioma_id":4,"titulo":"OPTIMIZATION OF THE PRODUCTION OF DIETARY FIBER CONCENTRATES FROM BY-PRODUCTS OF PAPAYA (CARICA PAPAYA L. VAR. FORMOSA) WITH MICROWAVE ASSISTANCE. EVALUATION OF ITS PHYSICOCHEMICAL AND FUNCTIONAL CHARACTERISTICS","resumen":"A process involving an ethanol extraction step and microwave assisted dehydration was designed to produce dietary fiber concentrates (DFC) from papaya (Carica papaya L. var. Formosa) by-products. We conclude that a 15 min extraction with 2.9 mL of ethanol\/g of papaya followed by drying performed at 40 °C, produced DFC with optimal values for functional properties (hydration properties, oil holding capacity, specific volume, water soluble fraction) as well as b* color parameter and content of phenolic compounds. The DFC obtained from papaya peel using the same conditions, showed a higher content of cell wall polysaccharides and higher glass transition temperature (38 °C) than the one obtained from pulp (8 °C) and the polyphenol content doubled the one of pulp. Both DFCs showed potential to be used for nutritional purposes as well as for technological applications as antioxidants and\/or thickeners.","palabras_clave":"FIBER|CARICA PAPAYA|FUNCTIONAL PROPERTIES|COLOR|PHENOLICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5522,"idioma_id":4,"titulo":"Impact of different factors on the yield and properties of fractions enriched in dietary fiber isolated from peach (Prunus persica L.) residues","resumen":"The industrialization of peaches gives origin to leftover remaining from fruit canning, juice or jam pro- duction. The transformation of leftover to dietary fiber (DF) concentrates represents a strategy that can be incor- porated in productive processes tending to optimize raw material use. In the present research, DF was obtained from peach (Prunus persica L.) using an ethanol treatment followed by dehydration. The influence of four factors: time and temperature of ethanol treatment prior to drying step, ratio of ethanol to sample and drying temperature using microwave or convective drying on different prop- erties (yield, hydration properties, oil holding capacity and apparent density) of the concentrate obtained, were studied through a two level orthogonal design. Yield ranged between 4.38-7.27 g\/100g for all the conditions explored. Time and temperature of the ethanol extraction affected properties when a subsequent convective drying was employed. The effect of the ethanol\/sample ratio and of the temperature of drying on oil holding capacity and hydration properties was mainly detected on samples dried by microwave technique. The results obtained provide insight into the effect of processing of plant residues on the properties of DF concentrates obtained and on their on their potential performance as ingredients or addi- tives for the food industry. It could be concluded that the drying technique is a key factor in relation to the proper- ties of dietary fiber enriched fractions isolated from peach. Microwave drying allowed to produce fractions with func- tional properties that can be modulated through the use of different relations of ethanol to sample ratio and drying temperatures. ","palabras_clave":"Dietary fiber enriched fractions|Convective drying|Microwaves|Functional properties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5523,"idioma_id":4,"titulo":"Expression analysis of cannabinoid receptors 1 and 2 in B cells during pregnancy and their role on cytokine production","resumen":"The endocannabinoid system consists in a family of lipids that binds to and activates cannabinoid receptors. There are two receptors so far described, the cannabinoid receptor 1 (CB1) and 2 (CB2). In the context of pregnancy, the endocannabinoid system was shown participates in different key aspects of reproductive events. B-lymphocytes are pleiotropic cells belonging to the adaptive arm of the immune system. Besides immunoglobulin production, B-lymphocytes were recently shown to be actively involved in antigen presentation as well as cytokine production, thus playing a central role in immunity. In this study we first aimed to characterize the expression of CB1 and CB2 receptors in B cells during pregnancy and then analyze the impact of their activation in term of cytokine production by B cells from pregnant and non-pregnant mice. We observed that the expression of CB1 and CB2 receptors in B-lymphocytes is differentially regulated during pregnancy. While CB2 expression is down regulated CB1 is augmented in B-lymphocytes of pregnant mice. Additionally, the treatment of activated B-lymphocytes with specific CB1 and CB2 agonists, showed a different response in term of cytokine production. Particularly, CB1 against boosted the production of the anti-inflammatory cytokine IL-10 by activated B-lymphocytes from pregnant mice.","palabras_clave":"B cells|Pregnancy|Endocannabinoid system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5524,"idioma_id":4,"titulo":"Influence of spanwise twisting and bending on lift generation in MAV-like flapping wings","resumen":"A numerical-simulation tool is developed that is well suited for modeling the unsteady and nonlinear aerodynamics of flying insects and small birds as well as biologically inspired flapping-wing micro air vehicles. The tool consists of a combination of: i) an aerodynamic model, which is an extension of the widely used three-dimensional general unsteady vortex-lattice model and ii) a general kinematic model that is capable of describing multiple deformation patterns of lifting surfaces, such as spanwise twisting, in-plane and out-of-plane bending, and any combination of these. Moreover, the present tool offers an attractive compromise between computational cost and fidelity and is ideally suited to be combined with computational structural dynamics to perform aeroelastic analyses. The present tool was successfully validated by comparing some of the present results with those obtained from existing numerical models based on both Euler equations and vortex-lattice codes as well as with some experimental data. By using the numerical framework developed and for the deformation mechanisms analyzed here, it was found two distinctly different effects: the wingspan?s twisting and in-plane bending affect the lift in specific zones of the stroke cycle (called ?local behavior?); and the wingspan?s out-of-plane bending affects the lift throughout the stroke cycle (called ?global behavior?). In addition, the results found show that wing?s flexibility certainly affect the lift production, at least for some flights at small scales. These findings definitely suggest the strong likelihood that the unsteady vortex-lattice method along with a general kinematic model could be a very accurate and efficient tool for future aeroelastic studies.","palabras_clave":"FLAPPING WINGS|MICRO-AIR VEHICLES|UNSTEADY AEROELASTICITY|UNSTEADY AERODYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5526,"idioma_id":5,"titulo":"Experiencias en transposición didáctica y socialización del conocimiento antropológico en el contexto museográfico de Tecnópolis","resumen":"Se realiza una reflexión crítica acerca de la comunicación pública y pedagógica del conocimiento antropológico en la Feria de Ciencias de Tecnópolis. La experiencia en ese lugar se transformó en una suerte de práctica etnográfica del público visitante, en cuyos discursos sobre la diversidad cultural fue percibida la reproducción de conceptos esencialistas y estereotipados, herederos de ideologías evolucionistas y etnocentristas.","palabras_clave":"COMUNICACIÓN PÚBLICA DE LA CIENCIA|EXTENSIÓN UNIVERSITARIA|MUSEOGRAFÍA|APRENDIZAJE SIGNIFICATIVO|ETNOGRAFÍA|ANTROPOLOGÍA|DESNATURALIZACIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5527,"idioma_id":5,"titulo":"Lo que nos contaron las plantas en los ultimos 30 años: Arqueobotánica relatada del Noroeste argentino","resumen":"Se repasa la historia de los últimos 30 años de la disciplina en el ámbito de las plantas domesticadas reconocidas en el registro arqueológico, mencionando los temas que han despertado la curiosidad de los investigadores  y sintetizando los resultados obtenidos acerca de los principales grupos de plantas utilizadas en el pasado prehispánico de la región.","palabras_clave":"ARQUEOBOTÁNICA|HISTORIOGRAFÍA DE LAS PLANTAS DOMESTICADAS|NOROESTE ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5528,"idioma_id":5,"titulo":"Historizando la Producción de Quinua en el valle de El Bolsón (Departamento Belén, Provincia de Catamarca).","resumen":"En este trabajo proponemos una interpretación histórica del cultivo de quinua en el Valle de El Bolsón. Para ello, recurrimos metodológicamente al análisis múltiple de microfósiles, a través del cual logramos reconocer tanto taxones cultivados como también las técnicas agrícolas implicadas en su producción. Así, en campos de cultivo formativosidentificamos microalmidones observados en C. quinoa spp., asociados en todos los casos al cultivo de Zea mays, ambos producidos a secano, con quema de rastrojos y labranza de campos. La continuidad en el tiempo del cultivo de quinua aparece sugerida a partir de su registro en un sitio del período de Desarrollos Regionales, nuevamente asociada al maíz pero con indicadores de riego y aportes de guano como abono. En algún momento de la historia, el cultivo de esta planta se interrumpió dado que hasta hace pocos años era desconocida tanto ella como su nombre entre los pobladores actuales. En el año 2010 vuelve a ser sembrada en el marco de un proyecto de reintroducción de cultivos andinos del INTA. Esta trayectoria histórica local, nos presenta distintos escenarios temporales en los cuales se habría planteado la relación entre los campesinos del valle y la quinua, relación que en la actualidad adquiere matices particulares en los cuales la quinua pasa a ser resignificada como cultivo novedoso, casi exótico.","palabras_clave":"quinoa|agricultura prehispanica|valle de el bolson"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5530,"idioma_id":4,"titulo":"Administration of Three Autochthonous Bacillus subtilis Strains Induce Early Appearance of Gastric Glands and Vestiges of Pylorus in Piaractus mesopotamicus Larvae","resumen":"Autochthonous microorganisms as probiotics are a novel alternative to antibiotics. The genus Bacillus is highly used by its ability to survive on adverse conditions. Piaractus mesopotamicus, a native fish, is the most produced specie in Argentinian aquaculture. The low level of knowledge, high stress conditions and demand of animals led to a deficit in the number of larvae and fry. The aim of this study was to evaluate the probiotic effect of previously selected autochthonous Bacillus on biometrical parameters of P. mesopotamicus larvae when administered together in a combined suspension in different doses and stages. Results showed that the bacterial administration did not cause significant effects on the variables evaluated. However, the dose containing 6 × 107 CFU L-1 of each strain showed the highest average values of P. mesopotamicus biological parameters, regardless the stage of administration. Histological evaluations of animals administered with this dose did not showed adverse effects and indicated the capability of this dose, administered in a particularly period of time, to stimulate the early appearance of gastric glands, vestiges of what would be the pylorus and a higher development of liver. Thus, we proposed the safe use of this dose in a combined probiotic mixture to be studied in further assays.","palabras_clave":"BACILLUS SUBTILIS|PIARACTUS MESOPOTAMICUS|PROBIOTICS|AQUACULTURE|LARVAE|BENEFICIAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5531,"idioma_id":4,"titulo":"In vivo evaluation of indigenous enterococci strains on biometrical parameters of Piaractus mesopotamicus embryos and larvae","resumen":"Abstract The use of autochthonous microorganisms as probiotics is a novel and promising alternative for the preventive application of antibiotics. Lactic acid bacteria (LAB) are one of the most successfully microorganisms used as probiotics and applied in different niches. The aquaculture of Piaractus mesopotamicus in Northern Argentina has increased exponentially, but the use of this native species in production systems led to a deficit in the availability of larvae for breeding and fattening. The objective of the present study was to evaluate the effect of the addition of two different formulae containing autochthonous beneficial LAB strains on the survival, mean weight and biomass of larvae under laboratory intensive breeding. Results indicate that the bacterial administration does not cause any significant effect on the variables evaluated. However, statistical analysis showed a tendency of the 6x107 CFUL-1 dose of one of the formula to stimulate an increase of mean weight and biomass. On the other hand, the average values of biometrical parameters obtained with the second formula, indicate that the optimum dose is 6x104 CFU L-1. These results and the histological confirmation of the harmfulness of the strains, allowed us to select these doses for their use in a combined probioticmixture to be tested in future assays.","palabras_clave":"Beneficial effects|Enterococcus faecium|Piaractus mesopotamicus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5532,"idioma_id":4,"titulo":"BRAINCASE AND NEUROANATOMY OF PSEUDOCHAMPSA ISCHIGUALASTENSIS AND TROPIDOSUCHUS ROMERI (ARCHOSAURIFORMES, PROTEROCHAMPSIA)","resumen":"Proterochampsids form a clade of archosauriforms endemic to South America, interpreted as the sister-taxon of the crown-group Archosauria. Because of this, aspects of&nbsp; the skull osteology in this group are particularly important to understand the evolutionary&nbsp; radiation of Archosauriformes. The braincase anatomy of archosauriforms was described&nbsp; for several groups, including few proterochampsids. However, paleoneurological studies on&nbsp; basal archosauriforms are scarce, and these were never conducted on proterochampsids,&nbsp; except for few preliminary analyses of Tropidosuchus romeri and Pseudochampsa&nbsp; ischigualastensis. Here we add descriptive information on the braincase and partial&nbsp; endocranial morphology of the type-specimens of the proterochampsids Pseudochampsa&nbsp; ischigualastensis and Tropidosuchus romeri based on CT scans. Reptile Encephalazation&nbsp; Quotient (REQ) and olfactory ratios were calculated for the first the time for a&nbsp; proterochampsid, indicating a high olfactory acuity for Tropidosuchus romeri, and&nbsp; suggesting a lifestyle similar to that of living crocodylians. The neurocranial information&nbsp; presented here is important due the scarcity of such data for Proterochampsidae.&nbsp;&nbsp; ","palabras_clave":"BRAINCASE|CRANIAL ENDOCAST|PROTEROCHAMPSID|EQ|OLFACTORY ACUITY|TRIASSIC|SOUTH AMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5533,"idioma_id":4,"titulo":"Genetic Linkage Of Capsid Protein-Encoding RNA Segments In Group A Equine Rotaviruses.","resumen":"Rotavirus virions are formed by a three concentric protein layers that enclose the 11 dsRNA genome segments and the viral proteins VP1 and VP3. Interactions amongst the capsid proteins (VP2, VP6, VP7 and VP4) have been described to play a major role in viral &#64257;tness, whilst restricting the reassortment of the genomic segments during co-infection with different rotavirus strains. In this work we describe and characterize the linkage between VP6 and VP7 proteins based on structural and genomic analyses of group A rotavirus strains circulating in Argentinean horses. Strains with the VP7 genotype G3 showed a strong association with the VP6 genotype I6, whilst strains with G14 were associated with the I2 genotype. Most of the differences on the VP6 and VP7 proteins were observed in interactive regions between the two proteins, suggesting that VP6:VP7 interactions may drive the co-evolution and co-segregation of their respective gene segments.","palabras_clave":"GENETIC LINKAGE|ROTAVIRUS|STRUCTURAL BIOLOGY|EQUINE DIARRHEA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5534,"idioma_id":4,"titulo":"Antiviral agents against equid alphaherpesviruses: Current status and perspectives","resumen":"Equid herpesvirus infections cause respiratory, neurological and reproductive syndromes. Despite preventive and control measures and the availability of vaccines and immunostimulants, herpesvirus infections still constitute a major threat to equine health and for the equine industry worldwide. Antiviral drugs, particularly nucleoside analogues and foscarnet, are successfully used for the treatment of human alphaherpesvirus infections. In equine medicine, the use of antiviral medications in alphaherpesvirus infections would decrease the excretion of virus and diminish the risk of contagion and the convalescent time in affected horses, and would also improve the clinical outcome of equine herpesvirus myeloencephalopathy. The combined use of antiviral compounds, along with vaccines, immune modulators, and effective preventive and control measures, might be beneficial in diminishing the negative impact of alphaherpesvirus infections in horses. The purpose of this review is to analyse the available information regarding the use of antiviral agents against alphaherpesviruses, with particular emphasis on equine alphaherpesvirus infections.","palabras_clave":"Antiviral agents|Alphaherpesviruses|Equine medicine|Equine herpesvirus diseases"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5535,"idioma_id":4,"titulo":"Palynology of the Late Triassic Ischigualasto Formation, Argentina: Paleoecological and paleogeographic implications","resumen":"In this study we describe and compare the palynological assemblages from the Carnian Ischigualasto Formation of westernmost Gondwana to those of eastern Gondwana, which have been referred to the Onslow and Ipswich phytogeographic provinces. The palynofloras studied contain many taxa common to both regions, as well as several species that are widely recorded in European Carnian successions and less frequently recovered in the Carnian of the Southern Hemisphere. For the first time in Argentinean assemblages, we recognize Anapiculatisporites spiniger, Cadargasporites granulatus, Cycadopites stonei, Ellipsovelatisporites plicatus, Enzonalasporites vigens, Ovalipollis pseudoalatus, O. ovalis, Patinasporites densus, Quadraeculina anellaeformis, Samaropollenites speciosus, Staurosaccites quadrifidus, ?Rimaesporites? aquilonalis, Vallasporites ignacii and Zonalasporites cinctus. The new evidence extends the distribution of the Onslow palynoflora to include Carnian associations formerly assigned to the Ipswich Province. The affinities of Argentinean palynofloras seem to indicate the existence of a similar flora extending from the northern Australian margin (West Timor), through the western Tethys coasts, to westernmost Gondwana during the Carnian. Moreover, the new data reinforce the close relationship between some diagnostic species and humid environmental conditions.","palabras_clave":"Carnian|Argentina|Ischigualasto|Palynology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5536,"idioma_id":4,"titulo":"Efficient edition of the bovine PRNP prion gene in somatic cells and IVF embryos using the CRISPR\/Cas9 system","resumen":"The recently developed engineered nucleases, such as zinc-finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeat (CRISPR)\/CRISPR-associated nuclease (Cas) 9, provide new opportunities for gene editing in a straightforward manner. However, few reports are available regarding CRISPR application and efficiency in cattle. Here, the CRISPR\/Cas9 system was used with the aim of inducing knockout and knock-in alleles of the bovine PRNP gene, responsible for mad cow disease, both in bovine fetal fibroblasts and in IVF embryos. Five single-guide RNAs were designed to target 875 bp of PRNP exon 3, and all five were codelivered with Cas9. The feasibility of inducing homologous recombination (HR) was evaluated with a reporter vector carrying EGFP flanked by 1 kbp PRNP regions (pHRegfp). For somatic cells, plasmids coding for Cas9 and for each of the five single-guide RNAs (pCMVCas9 and pSPgRNAs) were transfected under two different conditions (1X and 2X). For IVF zygotes, cytoplasmic injection was conducted with either plasmids or mRNA. For plasmid injection groups, 1 pg pCMVCas9 + 0.1 pg of each pSPgRNA (DNA2X) was used per zygote. In the case of RNA, two amounts (RNA1X and RNA2X) were compared. To assess the occurrence of HR, a group additionally cotransfected or coinjected with pHRegfp plasmid was included. Somatic cell lysates were analyzed by polymerase chain reaction and surveyor assay. In the case of embryos, the in vitro development and the genotype of blastocysts were evaluated by polymerase chain reaction and sequencing. In somatic cells, 2X transfection resulted in indels and large deletions of the targeted PRNP region. Regarding embryo injection, higher blastocyst rates were obtained for RNA injected groups (46\/103 [44.6%] and 55\/116 [47.4%] for RNA1X and RNA2X) than for the DNA2X group (26\/140 [18.6%], P < 0.05). In 46% (26\/56) of the total sequenced blastocysts, specific gene editing was detected. The total number of genetic modifications (29) was higher than the total number of gene-edited embryos, as three blastocysts from the group RNA2X reported more than one type of modification. The modifications included indels (10\/56; 17.9%) and large deletions (19\/56; 33.9%). Moreover, it was possible to detect HR in 1\/8 (12.5%) embryos treated with RNA2X. These results report that the CRISPR\/Cas9 system can be applied for site-specific edition of the bovine genome, which could have a great impact on the development of large animals resistant to important zoonotic diseases.","palabras_clave":"CATTLE|RUMINANT|BOVINE SPONGIFORM ENCEPHALOPATHY|MAD COW DISEASE|ENGINEERED NUCLEASE|GENE TARGETING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5537,"idioma_id":4,"titulo":"Weighted embedding theorems for radial Besov and Triebel-Lizorkin spaces","resumen":"We study the continuity and compactness of embeddings for radial Besov andTriebel-Lizorkin spaces with weights in the Muckenhoupt class A&#8734;. Themain tool is a discretization in terms of an almost orthogonal waveletexpansion adapted to the radial situation.","palabras_clave":"weighted embedding |besov |triebel-Lizorkin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5538,"idioma_id":4,"titulo":"The archaeological landscape of Late Prehispanic mixed foraging and cultivation economy (Sierras of Córdoba, Argentina)","resumen":"A short review of current data regarding the landscape use in the Sierras of Córdoba Late Prehispanic Period (ca. 1500?300 BP) is presented in this article. Resulting expectation about residential mobility and subsistence are analyzed in light of new evidence and interpretative framework. The lack of substantialmiddens, few evidences of year-round residence and the low investment in farming fields support the inadequacies of the assumption that the agriculture led to the sudden dependence of crops and to the sedentary way of life in pit-house villages. Other archaeological indicators as the intensity of landscapeuse, the taxonomic richness of food residues, the abundance of projectile point-types and isotopic evidence reinforce these arguments. However, the evidence suggests that the Late Prehispanic peoples showed flexible subsistence and mobility patterns as one of their defining traits, where nuclear familiesmoved around the landscape to take advantage of both agricultural and wild resources as available. Thus, farming was a fluctuating component in a mixed foraging and cultivation economy where wild animals and plants were intensively exploited through seasonal co-residential group fission?fusion mechanismsthat follow the pre-existing Middle Holocene forager lifeways. Nevertheless, many variables can affect this equation, including regional population densities, social boundaries or annual variation in foraging opportunities and a mosaic of strategies combination is also predicted.","palabras_clave":"Sierras of Córdoba|Late Prehispanic Period|Subsistence|Residential Mobility|Pit-House Village|Wild Resources|Farming"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5539,"idioma_id":4,"titulo":"Benthic fluxes and nitrate reduction activity in a marine park (Northern San Jorge Gulf) from Patagonia Argentina","resumen":"The Northern San Jorge Gulf (NSJG) wasdeclared Coastal Marine Park in 2008 with the goal ofpreserving its biodiversity. In situ benthic fluxes experimentswere performed using an opaque chamber in SaraCreek (SC) and Malaspina Creek (MC). Moreover, ex situnitrate reduction activity was assessed using a continuousflow-through system. Benthic fluxes in MC, showed aconsumption of oxygen (794 ± 196 mg m-2 h-1)and nitrate (103 ± 68 lmol m-2 h-1) by sediment andrelease of ammonium (175 ± 60 lmol m-2 h-1), phosphate(66 ± 10 lmol m-2 h-1) and silicate (116 ±66 lmol m-2 h-1) towards the water column. In SC, thesame pattern was observed and the fluxes values were375 ± 132, 128 ± 1, 76 ± 12, 39 ± 24 and 133 ± 81,respectively. Only, ammonium and oxygen fluxes showedsignificant differences between sites, due to the highestorganic matter content and the particular hydrodynamicconditions in MC. These are the first benthic flux measurementsmade within the protected area. Nitrate uptakerate, based on Michaelis?Menten kinetic, was Km:155.3 lM and Vmax: 0.053 lM g-1 h-1 in SC and Km:131.2 lM and Vmax: 0.039 lM g-1 h-1 in MC. Consideringthe nitrate dynamic we concluded that the sedimentsboth SC and MC act as a sink of nitrate. This ion could beprincipally used as a terminal electron acceptor during theoxidation process of organic compounds in sediments ofNSJG.","palabras_clave":"BENTHIC FLUX CHAMBER|SEDIMENT-WATER INTERFASE|NITRATE REDUCTION|CONTINUOS FLOW-THROUGH SYSTEM|MARINE PARK|ARGENTINEAN PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5540,"idioma_id":4,"titulo":"Antifungal effect of kefir fermented milk and shelf life improvement of corn arepas","resumen":"Fungal contamination negatively affects the production of cereal foods such as arepa loaf, an ancient corn bread consumeddaily in several countries of Latin-America. Chemical preservatives such as potassium sorbate are applied in orderto improve the arepa´s shelf life and to reduce the health risks. The use of natural preservatives such as natural fermentedproducts in food commodities is a common demand among the consumers. Kefir is a milk fermented beverage obtainedby fermentation of kefir grains. Its antibacterial and probiotic activity has been exhaustively demonstrated.Our objectives were to determine the antifungal effect of kefir fermented milk on Aspergillus flavus AFUNL5 in vitroand to study if the addition of kefir fermented milk to arepas could produce shelf life improvement.We determined the antifungal effect on solid medium of kefir cell-free supernatants (CFS) obtained under differentfermentation conditions. Additionally, we compared the antifungal effect of kefir CFS with that obtained with unfermentedmilk artificially acidified with lactic plus acetic acids (lactic and acetic acids at the same concentration determinedin kefir CFS) or with hydrochloric acid. Finally, kefir was added to the corn products either in the loaf recipe (kefir-bakedarepas) or sprayed onto the baked-loaf surface (kefir-sprayed arepas). The loaves´ resistance to natural and artificial fungalcontamination and their organoleptic profiles were studied.The highest fungal inhibition on solid medium was achieved with kefir CFS produced by kefir grains CIDCA AGK1at 100 g\/L, incubated at 30 °C and fermented until pH 3.3. Other CFS obtained from different fermentation conditionsachieved less antifungal activity than that mentioned above. However, CFS of milk fermented with kefir grains, untilpH 4.5 caused an increase of growth rates. Additionally, CFS produced by kefir grains CIDCA AGK1 at 100 g\/L, incubatedat 30 °C and fermented until pH 3.3 achieved higher antifungal activity than CFS from artificially acidified milkwith organic acids (CFS L + A) at the same concentration of kefir CFS. Besides, CFS from milk acidified with hydrochloricacid (CFS HCl) showed no fungal inhibition. On the other hand, kefir-baked arepas exhibited significant resistanceto natural and artificial fungal contamination. Finally, both kefir-baked and kefir-sprayed arepas retained the organolepticcharacteristics of the traditional corn product, but with certain tastes imparted by the kefir fermentation. This workconstitutes the first study on fungal inhibition by kefir-fermented milk extending to the protection of corn products ofmass-consumption and the possible application as a food preservative.","palabras_clave":"CORN AREPAS|KEFIR|ANTIFUNGAL EFFECT|PROBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5541,"idioma_id":4,"titulo":"Prevalence and antibiotic susceptibility of coagulase-negative Staphylococcus species from bovine subclinical mastitis in dairy herds in the central region of Argentina","resumen":"Coagulase-negative staphylococci (CNS) are a common cause of bovine subclinical mastitis (SCM). The prevalence of CNS species causing SCM identified by genotyping varies among countries. Overall, the antimicrobial resistance in this group of organisms is increasing worldwide; however, little information exists about a CNS species resistant to antibiotics. The aim of the present study was to genotypically characterize CNS at species level and to determine the prevalence and antibiotic resistance profiles of CNS species isolated from bovine SCM in 51 dairy herds located in the central region of the province of Cordoba, Argentina. In this study, we identified 219 CNS isolates at species level by PCR-restriction fragment length polymorphism of the groEL gene. Staphylococcus chromogenes (46.6%) and Staphylococcus haemolyticus (32%) were the most prevalent species. A minimum of three different CNS species were present in 41.2% of the herds. S. chromogenes was isolated from most of the herds (86.3%), whereas S. haemolyticus was isolated from 66.7% of them. The broth microdilution method was used to test in vitro antimicrobial susceptibility. Resistance to a single compound or two related compounds was expressed in 43.8% of the isolates. S. chromogenes and S. haemolyticus showed a very high proportion of isolates resistant to penicillin. Resistance to two or more non-related antimicrobials was found in 30.6% of all CNS. S. haemolyticus exhibited a higher frequency of resistance to two or more non-related antimicrobials than S. chromogenes.","palabras_clave":"Antimicrobial resistance|Coagulase-negative staphylococci species|Bovines|Subclinical mastitis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5542,"idioma_id":4,"titulo":"Personality-aware followee recommendation algorithms: An empirical analysis (Indexed SCI, IF JCR2014=2.207)","resumen":"As the popularity of micro-blogging sites, expressed as the number of active users and volume of online activities, increases, the difficulty of deciding who to follow also increases. Such decision might not depend on a unique factor as users usually have several reasons for choosing whom to follow. However, most recommendation systems almost exclusively rely on only two traditional factors: graph topology and user-generated content, disregarding the effect of psychological and behavioural characteristics, such as personality, over the followee selection process. Due to its effect over people´s reactions and interactions with other individuals, personality is considered as one of the primary factors that influence human behaviour. This study aims at assessing the impact of personality in the accurate prediction of followees, beyond simple topological and content-based factors. It analyses whether user personality could condition followee selection by combining personality traits with the most commonly used followee predictive factors. Results showed that an accurate appreciation of such predictive factors tied to a quantitative analysis of personality is crucial for guiding the search of potential followees, and thus, enhance recommendations. © 2016 Elsevier Ltd. All rights reserved.","palabras_clave":"FOLLOWEE RECOMMENDATION|TWITTER|PERSONALITY TRAITS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5543,"idioma_id":4,"titulo":"Myology of the head, neck and thoracic region of the lesser grison (Galictis cuja) in comparison with the red panda (Ailurus fulgens) and other carnivorans: phylogenetic and functional implications","resumen":"The lesser grison (Galictiscuja) and the red panda (Ailurusfulgens) represent two opposed morpho-functional musteloid extremes. Themid-sized lesser grison is primarily terrestrial, a frequent burrow-dweller, and carnivorous, while the larger, scansorial red pandaeats bamboo. This study documents the axial myology of these species,including muscle descriptions, weights, and optimizations. Muscle maps are alsoprovided for the lesser grison, representing the first axial maps for awild-caught carnivoran. The functional analyses revealed that G. cuja, contrary to A. fulgens, possesses longer, stronger,and subdivided neck muscles. It also possesses a thoraco-lumbar iliocostalissystem that is more developed than the longissimus complex; and numerous, robust,and laterally inserted deep bellies of the cervical and thoracictransversospinalis systems. These adaptations allow powerful neck movementduring hunting and transport of heavy prey as well as axial flexibility,facilitating boundinggaits and lateral movements while navigating subterranean galleries. Some myological traits of the red panda differfrom those expected in a highly herbivorous taxon (e.g., m. sternocephalicus,m. masseter),and may reflect its depredatory ancestry. The optimizationanalysis revealed phylogenetically informative traits across Carnivora,including the absence of m. longissimus capitis in Mephitidae, the absence ofspinous thoracic origins for m. biventer cervicis in Musteloidea, and thepresence of a relatively lateral insertion of m. rectus dorsalis capitisintermedius in the clade Ictonychinae+Lutrinae+Mustelinae. This study revealskey associations between axial myological and osteological adaptations thatwill prove useful for future studies of carnivorans.","palabras_clave":"AILURUS|CARNIVORA|GALICTIS|AXIAL MYOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5544,"idioma_id":4,"titulo":"Functional Thioredoxin Reductase from Pathogenic and Free-living Leptospira spp.","resumen":"Low molecular mass thiols and antioxidant enzymes have essential functions to detoxify reactive oxygen and nitrogen species maintaining cellular redox balance. The metabolic pathways for redox homeostasis in pathogenic (Leptospira interrogans) and free-living (Leptospira bi&#64258;exa) leptospires species were not functionally characterized. We performed biochemical studies on recombinantly produced proteins to depth analyze kinetic and structural properties of thioredoxin reductase (LinTrxR) and thioredoxin (LinTrx) from L. interrogans, and two TrxRs (LbiTrxR1 and LbiTrxR2) from L. bi&#64258;exa. All the TrxRs were characterized as homodimeric &#64258;avoproteins, with LinTrxR and LbiTrxR1 catalyzing the NADPH dependent reduction of LinTrx and DTNB. The thioredoxin system from L. interrogans was able to use glutathione disul&#64257;de, lipoamide disul&#64257;de, cystine and bis&#947;-glutamyl cysteine and homologous peroxiredoxin substrates. Classic TrxR activity of LinTrxR2 had not been evidenced in vitro, but recombinant Escherichia coli cells overexpressing LbiTrxR2 showed high tolerance to oxidative stress. The enzymatic systemsherein characterized could play a key role for the maintenance of redox homeostasis and the function defense mechanisms against reactive oxidant species in Leptospira spp. Our results contribute to the general knowledge about redox biochemistry in these bacteria, positioning TrxR as a critical molecular target for the development of new anti-leptospiral drugs.","palabras_clave":"Leptospira|Thioredoxin reductase|Peroxiredoxin|Redox metabolism"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5545,"idioma_id":4,"titulo":"Improved model for the thermal performance calculation of non-planar window frames for building simulation programs","resumen":"Windows are a key factor for designing energy efficient buildings, particularly the frame area that can produce high thermal bridging. This paper deals with accurately estimating heat transfer through window frames under fluctuating film coefficients. The one-dimensional frame conductance model traditionally used by building simulation programs is analyzed and an alternative model is proposed, which takes into account the non-planar morphology of studied frames. This model shows a positive agreement with the results obtained from a two-dimensional heattransfer simulation program, demonstrating that the thermal performance of highconductance non-planar frames strongly depends on the ratio between the boundary surface area and the projected frame area. According to the results, the traditional conductance model seems to be suitable for all frames with a thermaltransmittance lower than 5 W\/m2K; however, frames with a U-factor higher than 6.2 W\/m2K need an alternative conductance model that better reflects the 2D nature of frame sections.","palabras_clave":"THERMAL CONDUCTANCE|WINDOW FRAME|FILM COEFFICIENT|FRAME COMPACTNESS|SIMULATION|U-FACTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5546,"idioma_id":4,"titulo":"The MeSsI (Merging Systems Identification) Algorithm & Catalogue","resumen":"Merging galaxy systems provide observational evidence of the existence of dark matter and constraints on its properties. Therefore, statistically uniform samples of merging systems would be a powerful tool for several studies. In this paper, we present a new methodology for the identification of merging systems and the results of its application to galaxy redshift surveys.We use as a starting point amock catalogue of galaxy systems, identified using friendsof- friends algorithms, that have experienced a major merger, as indicated by its merger tree. By applying machine learning techniques in this training sample, and using several features computed from the observable properties of galaxy members, it is possible to select galaxy groups that have a high probability of having experienced a major merger. Next, we apply a mixture of Gaussian techniques on galaxy members in order to reconstruct the properties of the haloes involved in such mergers. This methodology provides a highly reliable sample of merging systems with low contamination and precisely recovered properties. We apply our techniques to samples of galaxy systems obtained from the Sloan Digital Sky Survey Data Release 7, theWide-Field Nearby Galaxy-Cluster Survey (WINGS) and the Hectospec Cluster Survey (HeCS). Our results recover previously known merging systems and provide several new candidates. We present their measured properties and discuss future analysis on current and forthcoming samples.","palabras_clave":"DARK MATTER|GALAXIES: CLUSTERS|GALAXIES: KINEmATICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5547,"idioma_id":4,"titulo":"Evaluation and synthesis of AZT prodrugs with optimized chemical stabilities. Experimental and theoretical analyses","resumen":"The design of prodrugs of nucleoside reverse transcriptase inhibitors (NRTIs) constitutes a promising strategy to overcome several suboptimal pharmacotherapeutic properties of these kinds of drugs, among which zidovudine (AZT) is the most studied example. The chemical stability of prodrugs is a critical issue in the context of their pharmacotherapeutic performance since it constitutes a key event in the reconversion of the bioactive NRTIs. In this study, five prodrugs of AZT and lamivudine (3TC) were studied by means of DFT and classical molecular dynamics (MD) strategies in order to model the reaction coordinates involved in their chemical hydrolysis, and extend these conclusions to further structure rationalization of prodrugs. Therefore, the inclusion of explicit water molecules was found to be of great relevance to the mentioned reaction coordinates since it allowed a very good correlation between calculated reaction energy values and the corresponding hydrolysis constants. Based on these findings, three ester based prodrugs of AZT were designed, synthesized and evaluated regarding their chemical stability. These prodrugs exhibited optimized chemical stabilities when compared to the previously reported AZT prodrugs, which may result in an enhanced pharmacotherapeutic performance. In conclusion, the developed theoretical model provides a valuable assistance in the design and development of novel prodrugs of NTRIs with rationalized chemical stabilities","palabras_clave":"AZT|Produgs|chemical stabilities|theoretical analyses"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5548,"idioma_id":4,"titulo":"Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively","resumen":"Entomopathogens play a significant role in regulating insect populations in the field, and their use in pest management is the subject of active research. A variety of classical and molecular techniques is currently available to elucidate the etiology of diseases and to better understand pathogenesis. Here, we performed a modified Azan trichrome stain for light microscopic visualization of pathogens in the larval tissues of two Neotropical lepidopterans. Epinotia aporema larvae exhibiting symptoms of microsporidiosis were fortuitously detected in a rearing facility (IMyZA-INTA, Buenos Aires, Argentina), while Helicoverpa gelotopoeon specimens were experimentally infected with an Argentinean isolate of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Caterpillars were fixed in Duboscq-Brasil and embedded in paraffin. After cutting with a microtome, 5 &#956;m-thick sections were deparaffinized and stained according to Hamm2. Observations were made at 4X - 100X in a Nikon Eclipse 80i microscope equipped with a Nikon DS-U3 digital camera. Diseased E. aporema samples revealed the occurrence of microsporidians occupying a large portion of the body cavity. Typical oval spores were stained bright red. No microsporidians were identified in the healthy controls (not shown). This confirmed preliminary diagnosis and is, to our knowledge, the first report of a microsporidian infection in E. aporema. These microbial pathogens are widespread among Lepidoptera, and their ability to undergo both vertical and horizontal transmission can cause a serious problem for insect mass rearing3. Regarding H. gelotopoeon, cytological examinations evidenced the presence of numerous polyhedral occlusion bodies, also displaying a bright red color, within the nuclei of HearNPV-infected cells. Several larval tissues were shown to be colonized, including epidermis, fat bodies and tracheal system, thus indicating the polyorganotropic nature of the virus isolate. No polyhedra were found in the negative controls, where staining ranged from yellowish-green (with darker green nuclei) to blue, depending on the tissue. Our findings are complementary to a previous work on this new baculovirus isolate, in which we described the ultrastructure of viral particles and occlusion bodies by electron microscopy1. Overall, the Azan staining technique applied to microtome sections proved to be a useful accessory tool to identify and localize different types of pathogens within lepidopteran larvae, as part of broader characterization studies.","palabras_clave":"Light microscopy|Microsporidia|Nucleopolyhedrovirus|Lepidoptera"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5549,"idioma_id":4,"titulo":"First record of the nasal mite Rallinyssus caudistigmus Strandtmann (Acari: Rhinonyssidae) from Argentina","resumen":"During an examination of the parasite fauna of the Red-gartered Coot Fulica armillata from the Mari Menuco lake in the Northwestern Argentinean Patagonia, nasal mite specimens belonging to Rallinyssus caudistigmus were found. This is the southernmost record of R. caudistigmus in F. armillata as a new host.","palabras_clave":"Nasal mites|Fulica armillata|Rallinyssus|Taxonomy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5550,"idioma_id":4,"titulo":"Micropropagation of Handroanthus heptaphyllus (Vell.)  Mattos from seedling explants","resumen":"Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidal properties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfection protocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 µM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots without shoot tip necrosis compared with culture on semisolid medium. Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of Handroanthus heptaphyllus.","palabras_clave":"Seeds,     |adventitious shoot, |uncoated seeds,|cotyledon,|stem nodes,|axillary shoots,|black lapacho."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5551,"idioma_id":4,"titulo":"Decoding the chemokine network that links leukocytes with decidual cells and the trophoblast during early implantation","resumen":"Chemokine network is central to the innate and adaptive immunity and entails a variety of proteins and membrane receptors that control physiological processes such as wound healing, angiogenesis, embryo growth and development. During early pregnancy, the chemokine network coordinates not only the recruitment of different leukocyte populations to generate the maternal-plancental interface, but also constitutes an additional checkpoint for tissue homeostasis maintenance. The normal switch from a pro-inflammatory to an anti-inflammatory predominant microenvironment characteristic of the post-implantation stage requires redundant immune tolerance circuits triggered by key master regulators. In this review we will focus on the recruitment and conditioning of maternal immune cells to the uterus at the early implantation period with special interest on high plasticity macrophages and dendritic cells and their ability to induce regulatory T cells. We will also point to putative immunomodulatory polypeptides involved in immune homeostasis maintenance at the maternal-placental interface.","palabras_clave":"pregnancy|maternal tolerance|chemokines"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5552,"idioma_id":4,"titulo":"Population genetic structure and historical dispersal  patterns in the subterranean rodent Ctenomys 'chasiquensis' from the  southeastern Pampas region, Argentina.","resumen":"Gene flow plays an essential role in the evolutionary history of the organisms and helps toidentify those historical landscape features that most likely had affected the dispersal patterns.In this work, we assess, using mitochondrial control region DNA sequences, the historicalmigration patterns and population structure in C. ?chasiquensis?, a highly, vulnerable andendemic subterranean rodent distributed in a very small area from the central part of Argentina.We used Bayesian and maximum likelihood approaches to evaluate the effects of historical geneflow among populations. Moreover, we used Bayesian skyline plots, tests of neutrality andmismatch distributions to assess the potential changes in population size through time. Ouranalyses show that populations of C. ?chasiquensis? are moderate structured at regional leveland this population pattern is probably the result of an asymmetric historical gene flowessentially from the South-West to the North-West, further of a recent demographic populationexpansion in the North-West, in conjunction with an important degree of isolation in somepopulations over its eastern geographical range. Evidently, historical gene flow seems to havebeen more frequently on the West. Finally, a close relationship appears to exist between themajor climatic episodes occurred during the Late Quaternary in the central region of Argentinaand the main historical demographic changes inferred for C. ?chasiquensis?. The currentdistribution of C. ?chasiquensis? appear to be a relicts of a more extended historical distributionin the Argentinean Pampas in the Late Pleistocene, with a perceptible population decline at thebeginning of the Holocene. In this context, Bayesian demographic inferences showed a smallbut constant increment of population expansion of this species from approximately 90.000 to11,000 years BP, after which a period of decrease in population size (that started in the earlyHolocene and continues nowadays) was observed.","palabras_clave":"Ctenomys chasiquensis|POPULATION GENETICS|HISTORICAL DISPERSAL PATTERNS|SUBTERRANEAN RODENTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5554,"idioma_id":5,"titulo":"Migración e identidad: El caso de Tierra del Fuego","resumen":"La hipótesis que intentamos demostrar en este trabajo, a partir de los datos recopilados, es que en Tierra del Fuego, uno de los rasgos distintivos a partir de los cuales se establece un límite entre un nosotros y un otro, está fuertemente marcado por la permanencia. Para ello partiremos de las siguientes preguntas: ¿Quién es, o puede ser, considerado ?fueguino?? ¿Cuáles son los elementos que identifican ?y son identificados por- los grupos sociales dentro de la comunidad? ¿Cómo están conformados esos límites sociales? ¿De qué manera estos límites involucran, generan o sostienen diferenciales de poder?En este trabajo nos proponemos aproximarnos a las formas particulares que toman en Tierra del Fuego ciertas categorías de identidad, clasificación y jerarquización social que aparecen en distintos discursos y prácticas de los habitantes de la provincia.","palabras_clave":"Tierra del Fuego|migración, |poder, |configuración cultural."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5555,"idioma_id":4,"titulo":"Cryptosporidium varanii infection in leopard geckos (Eublepharis macularius) in Argentina","resumen":"Cryptosporidiosis is observed in reptiles with high morbidity and considerable mortality. The objective of this study was to achieve the molecular identification of Cryptosporidium spp. in pet leopard geckos (Eublepharis macularius) from a breeder colony in Buenos Aires, Argentina. Oocysts comparable to those of Cryptosporidium spp. were detected in three geckos with a history of diarrhea, anorexia and cachexia. Molecular identification methods confirmed thepresence of Cryptosporidium varanii (syn. C. saurophilum). This agent was considered to be the primary cause of the observed clinical disease. This is the first description of C. varanii infection in pet reptiles in Argentina.","palabras_clave":"ARGENTINA|CRYPTOSPORIDIUM SPP.|LEOPARD GECKO|PCR|SEQUENCING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5556,"idioma_id":4,"titulo":"Vasoactive Intestinal Peptide modulates trophoblast-derived cell line function and interaction with phagocytic cells through autocrine pathways","resumen":"Trophoblast cells migrate and invade the decidual stroma in a tightly regulated process to maintainimmune homeostasis at the maternal-placental interface during the first weeks of pregnancy. Locallysynthesized factors modulate trophoblast cell function and their interaction with maternal leukocytesto promote the silent clearance of apoptotic cells. The vasoactive intestinal peptide (VIP) is a pleiotropicpolypeptide with trophic and anti-inflammatory effects in murine pregnancy models. We exploredthe effect of VIP on two human first trimester trophoblast cell lines, particularly on their migration,invasiveness and interaction with phagocytic cells, and the signalling and regulatory pathwaysinvolved. We found that VIP enhanced trophoblast cell migration and invasion through the activationof high affinity VPAC receptors and PKA-CRE signalling pathways. VIP knocked-down trophoblast cellsshowed reduced migration in basal and leukemic inhibitor factor (LIF)-elicited conditions. In parallel,VIP-silenced trophoblast cells failed to induce the phagocytosis of apoptotic bodies and the expressionof immunosuppressant markers by human monocytes. Our results suggest that VIP-mediated autocrinepathways regulate trophoblast cell function and contribute to immune homeostasis maintenance atplacentation and may provide new clues for therapeutic intervention in pregnancies complicated bydefective deep placentation.","palabras_clave":"VIP|Phagocytosis|trophoblast cells"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5557,"idioma_id":4,"titulo":"Bacterial diversity in microbial mats and sediments from the Atacama Desert","resumen":"The Atacama Desert has extreme environmental conditions that allow the development of unique microbial communities. The present paper reports the bacterial diversity of microbial mats and sediments and its mineralogical components. Some physicochemical conditions of the water surrounding these ecosystems have also been studied trying to determine their influence on the diversity of these communities. In that way, mats and sediments distributed among different hypersaline lakes located in salt flats of the Atacama Desert were subjected to massive parallel sequencing of the V4 region of the 16S rRNA genes of Bacteria. A higher diversity in sediment than in mat samples have been found. Lakes that harbor microbial mats have higher salinity than lakes where mats are absent. Proteobacteria and\/or Bacteroidetes are the major phyla represented in all samples. An interesting item is the finding of a low proportion or absence of Cyanobacteria sequences in the ecosystems studied, suggesting the possibility that other groups may be playing an essential role as primary producers in these extreme environments. Additionally, the large proportion of 16S rRNA gene sequences that could not be classified at the level of phylum indicates potential new phyla present in these ecosystems.","palabras_clave":"MICROBIAL DIVERSITY|ATACAMA DESERT|MICROBIAL MATS|SEDIMENTS|HYPERSALINE LAKES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5558,"idioma_id":4,"titulo":"Draft Genome Sequence of Achromobacter sp. Strain AR476-2, Isolated from a Cellulolytic Consortium","resumen":"Achromobacter sp. AR476-2 is a noncellulolytic strain previously isolated from a cellulolytic consortium selected from samplesof insect gut. Its genome sequence could contribute to the unraveling of the complex interaction of microorganisms and enzymesinvolved in the biodegradation of lignocellulosic biomass in nature.","palabras_clave":"Achromobacter sp.|Cellulolytic consortium|Genome"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5559,"idioma_id":4,"titulo":"Indirect facilitation becomes stronger with seedling age in a degraded seasonally dry forest","resumen":"In seasonally dry forests direct facilitation by woodyspecies due to amelioration of harsh abiotic conditions could be important during germination and earlyestablishment of tree seedlings, and under some species but not others. Recentresearch suggests that at later stages facilitation by woody species may be indirectdue to protection of saplings from herbivores, implying that under absence ofherbivores reforestation programs may plant saplings in unprotected open sites.We used the native tree Lithraea molleoides from central Argentina as a model species to testthis hypothesis. We performed a seeding and planting experiment simulatingearly and late establishment respectively, which included 234 study plotssituated in herbaceous, shrub and tree patches ofdiffering species composition and under two herbivore treatments (grazed andungrazed) and replicated at three sites. Seedling counts averaged 0.82% of the sown seeds after6 months, were highest under shrubs and lowest in open patches, and wereinfluenced by woody species composition only in tree patches (all P values&lt;0.05).At seedling stages we detected no influence of herbivory (P=0.4) nor of indirectfacilitation due to herbivory (herbivory×patch type P=0.7). Survival of plantedsaplings was 53% after 3 years and over winter dieback affected 76% of thesaplings. At saplingstages we found an increasing importance of indirect facilitation throughprotection from herbivores, as we recorded the highest saplingsurvival and growth at tree and shrub patches and the lowest in open patches(all P values&lt;0.001), and a negative effect of livestock (P&lt;0.001) mainlyon the open patches (herbivory×patch type P=0.07 and P=0.001 for survival andgrowth, respectively). We found nosignificant influence of woody species composition on sapling survival andgrowth (all P values&gt;0.05). We conclude that direct facilitation is involved at allstudied stages while indirect facilitation becomes increasingly important atthe sapling stage. ","palabras_clave":"Facilitation|Vegetation patch types|Livestock|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5560,"idioma_id":4,"titulo":"Climate change and the distribution and conservation of the world's highest elevation woodlands in the South American Altiplano.","resumen":"Climate change is becoming an increasing threat to biodiversity. Consequently,the methods for delineation, establishment and management of protected areasmust consider the species? future distribution in response to future climateconditions. Biodiversity in high altitude semiarid regions may be particularlythreatened by future climate change. In this study we assess the mainenvironmental variables that best explain present day presence of the world?s highest elevation woodlands in theSouth American Altiplano, and model how climate change may affect the future distribution of this uniqueecosystem under climate change scenarios. These woodlands are dominatedby Polylepis tarapacana trees, aframework species which forms unique biological communities with importantconservation value. Our results indicate that five environmental variables areresponsible for 91% and 90.3% of present and future P. tarapacana distribution models respectively, and suggest that atthe end of the 21st century there will be a reduction of 56% in the potential habitatof this species due to more arid conditions. Since it is predicted that P. tarapacana?s potential distributionarea will be severely reduced in the future, we propose a new network ofnational protected areas across this species distribution area to insure thefuture conservation of this unique ecosystem. Based on an extensive literaturereview we identify research topics and recommendations for the on-ground conservationand management of the P. tarapacanawoodlands.","palabras_clave":"Polylepis tarapacana|models|potential distribution|MaxEnt"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5561,"idioma_id":5,"titulo":"Interlaboratory test: Isolation of Mycobacterium bovis from granulomatous lesions in bovine","resumen":"Mycobacterium bovis is the causative agent of bovine tuberculosis. The diagnostic laboratory confirmation is made through bacterial isolation. The aim of interlaboratory tests is to assess the performance of each participant in comparison with other of similar capacities. The test objective was to determine the efficiency of isolation of M. bovis. Four laboratories were part of the test and processed 25 blind tissue samples from granulomatous lesions and with previous M. bovis isolation. The laboratory that had the highest proportion of isolates was A (68%), followed by C (60%) and then B and D (both with 52%). The greatest concordance was observed between B-D and B-C laboratories (68%). The differences could be due to specific factors in each laboratory procedures. This type of interlaboratory tests highlights errors in the bacteriology and identifies critical points in the process to detect M. bovis accurately.","palabras_clave":"Aislamiento; |Isolation; |Tuberculosis bovina|Mycobacterium bovis;"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5562,"idioma_id":4,"titulo":"Genome Sequence of Acidovorax avenae Strain T10_61 Associated with Sugarcane Red Stripe in Argentina","resumen":"Red stripe of sugarcane in Argentina is a bacterial disease caused by Acidovorax avenae. The genome sequence from the first isolate of this bacterium in Argentina is presented here. The draft genome of the A. avenae T10_61 strain contains 5,646,552 bp and has a GC content of 68.6 mol%.","palabras_clave":"Acidovorax avenae|sugarcane redstripe"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5563,"idioma_id":4,"titulo":"Biodiversity and technological-functional potential of lactic acid bacteria isolated from spontaneously fermented quinoa sourdoughs","resumen":"AIMS:To analyse lactic acid bacteria (LAB) diversity and technological-functional and safety properties of strains present during spontaneous fermented quinoa sourdoughs.METHODS AND RESULTS:Fermentation was performed by daily backslopping at 30°C for 10 days. Autochthonous LAB microbiota was monitored by a biphasic approach combining random amplified polymorphic DNA (RAPD)-PCR and rRNA gene sequencing with PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Identification and intraspecies differentiation allowed to group isolates within nine LAB species belonging to four genera. A succession of LAB species occurred during 10-days backslopping; Lactobacillus plantarum and Lactobacillus brevis were detected as dominant species in the consortium. The characterization of 15 representative LAB strains was performed based on the acidifying capacity, starch and protein hydrolysis, &#947;-aminobutyric acid and exopolysaccharides production, antimicrobial activity and antibiotic resistance.CONCLUSION:Strains characterization led to the selection of Lact. plantarum CRL1905 and Leuconostoc mesenteroides CRL1907 as candidates to be assayed as functional starter culture for the gluten-free (GF) quinoa fermented products.SIGNIFICANCE AND IMPACT OF THE STUDY:Results on native LAB microbiota present during quinoa sourdough fermentation will allow the selection of strains with appropriate technological properties to be used as a novel functional starter culture for GF-fermented products","palabras_clave":"quinoa sourdough|lactic acid bacteria|gluten free"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5564,"idioma_id":4,"titulo":". Biodiversity and technological potential of lactic acid bacteria isolated from spontaneously fermented amaranth sourdough.","resumen":"Signi&#64257;cance and Impact of the Study: Nowadays, there is an increasing interest in ancient noncerealgluten-free (GF) crops such as amaranth, due to their reported nutritional and health bene&#64257;ts. How-ever, the use of these grains is still limited to traditional foods and bread making processes that are notyet well standardized. Results on the dynamics of autochthonous lactic acid bacteria (LAB) microbiotaduring laboratory spontaneous amaranth sourdoughs (AS) fermentation will contribute to overcomechallenges for GF-fermented products development. In addition, knowledge about LAB diversity involv-ing Enterococcus, Pediococcus and Lactobacillus species, with Lactobacillus plantarum predominatingduring AS fermentation, and their technological and functional properties provides the basis for theselection of autochthonous strains as starters cultures for novel gluten-free bakery products withenhanced nutritional, sensory and\/or safety quality.","palabras_clave":"LACTIC ACID BACTERIA|SOURDOUGH|AMARANTH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5565,"idioma_id":4,"titulo":"Calpain-dependent truncated form of TrkB-FL increases in neurodegenerative processes","resumen":"Recent findings indicate that the mechanisms that drive reshaping of the nervous system are aberrantly activated in epilepsy and several neurodegenerative diseases. The recurrent seizures in epilepsy, particularly in the condition called status epilepticus, can cause permanent neurological damage, resulting in cognitive dysfunction and other serious neurological conditions. In this study, we used an in vitro model of status epilepticus to examine the role of calpain in the degeneration of hippocampal neurons. We grew neurons on a culture system that allowed studying the dendritic and axonal domains separately from the cell bodies. We found that a recently characterized calpain substrate, the neurotrophin receptor TrkB, is cleaved in the dendritic and axonal domain of neurons committed to die, and this constitutes an early step in the neuronal degeneration process. While the full-length TrkB (TrkB-FL) levels decreased, the truncated form of TrkB (Tc TrkB-FL) concurrently increased, leading to a TrkB-FL\/Tc TrkB-FL imbalance, which is thought to be causally linked to neurodegeneration. We further show that the treatment with N-acetyl-Leu-Leu-norleucinal, a specific calpain activity blocker, fully protects the neuronal processes from degeneration, prevents the TrkB-FL\/Tc TrkB-FL imbalance, and provides full neuroprotection. Moreover, the use of the TrkB antagonist ANA 12 at the time when the levels of TrkB-FL were significantly decreased, totally blocked neuronal death, suggesting that Tc TrkB-FL may have a role in neuronal death. These results indicate that the imbalance of these neurotrophins receptors plays a key role in neurite degeneration induced by seizures.","palabras_clave":"status epilepticus|neuronal death|TrkB-FL|calpain"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5566,"idioma_id":4,"titulo":"Alterations of hemorheological parameters and tubulin content in erythrocytes from diabetic subjects","resumen":"Treatment of human erythrocytes with high glucose concentrations altered the content and distributions of three tubulin isotypes, with consequent reduction of erythrocyte deformability and osmotic resistance. In erythrocytes from diabetic subjects (D erythrocytes), (i) tubulin in the membrane-associated fraction (Mem-Tub) was increased and tubulin in the sedimentable fraction (Sed-Tub) was decreased, (ii) deformability was lower than in erythrocytes from normal subjects (N erythrocytes), and (iii) detyrosinated\/acetylated tubulin content was higher in the Mem-Tub fraction and tyrosinated\/acetylated tubulin content was higher in the Sed-Tub fraction, in comparison with N erythrocytes. Similar properties were observed for human N erythrocytes treated with high glucose concentrations, and for erythrocytes from rats with streptozotocin-induced diabetes. In N erythrocytes, high-glucose treatment caused translocation of tubulin from the Sed-Tub to Mem-Tub fraction, thereby reducing deformability and inducing acetylation\/tyrosination in the Sed-Tub fraction. The increased tubulin acetylation in these cells resulted from inhibition of deacetylase enzymes. Increased tubulin acetylation and translocation of this acetylated tubulin to the Mem-Tub fraction were both correlated with reduced osmotic resistance. Our findings suggest that (i) high glucose concentrations promote tubulin acetylation and translocation of this tubulin to the membrane, and (ii) this tubulin is involved in regulation of erythrocyte deformability and osmotic fragility.","palabras_clave":"Tubulin|Diabetes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5567,"idioma_id":4,"titulo":"Evaluation of the antimicrobial efficacy of the Minthostachys verticillata essential oil and one of its main compounds, limonene, against Streptococcus uberis strains isolated from bovine mastitis","resumen":"La mastitis bovina es una enfermedad que causa grandes pérdidas económicas pora?no, Streptococcus uberis es el principal patógeno ambiental involucrado. El objetivo del pre-sente estudio fue determinar la concentración inhibitoria mínima (CIM) y la concentraciónbactericida mínima (CBM) del aceite esencial de Minthostachys verticillata y del limonenosobre cepas de S. uberis aisladas de mastitis bovina. Además, se analizó el efecto del aceiteesencial y el limoneno en la CIM determinada en caso sobre la formación de biofilm de estascepas. Los valores de CIM del aceite esencial oscilaron entre 14,3 y 114,5 mg\/ml (1,56%-12,5%v\/v) y los de CBM entre 114,5 y 229 mg\/ml (12,5%-25% v\/v). Las CIM del limoneno oscilaronentre 3,3 y 52,5 mg\/ml (0,39% - 6,25% v\/v) y la CBM fue de 210 mg\/ml (25% v\/v). Ambos compuestos mostraron actividad antibacteriana y afectaron la formación de biofilm de la mayoríade las cepas. En conclusión, estos compuestos podrían ser utilizados como terapia alternativa o complementaria para la mastitis bovina causada por S. uberis.","palabras_clave":"STREPTOCOCCUS UBERIS|MINTHOSTACHYS VERTICILLATA|ESSENTIAL OIL|LIMONENE|BOVINE MASTITIS|ANTIBACTERIAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5568,"idioma_id":4,"titulo":"A spectral envelope approach towards e ff ective SVM-RFE on infrared data","resumen":"Infrared spectroscopy data is characterized by the presence of a huge number of variables. Applications of infrared spectroscopy in the mid-infrared (MIR) and near-infrared (NIR) bands are of widespread use in many fields. To effectively handle this type of data, suitable dimensionality reduction methods are required. In this paper, a dimensionality reduction method designed to enable effective Support Vector Machine Recursive Feature Elimination (SVM-RFE) on NIR\/MIR datasets is presented. The method exploits the information content at peaks of the spectral envelope functions which characterize NIR\/MIR spectra datasets. Experimental evaluation across different NIR\/MIR application domains shows that the proposed method is useful for the induction of compact and accurate SVM classifiers for qualitative NIR\/MIR applications involving stringent interpretability or time processing requirements.","palabras_clave":"Spectral envelope|SVM-RFE|Infrared spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5569,"idioma_id":4,"titulo":"A Factor Graph Approach to Automated GO Annotation","resumen":"As volume of genomic data grows, computational methods become essential for providing a first glimpse onto gene annotations. Automated Gene Ontology (GO) annotation methods based on hierarchical ensemble classification techniques are particularly interesting when interpretability of annotation results is a main concern. In these methods, raw GO-term predictions computed by base binary classifiers are leveraged by checking the consistency of predefined GO relationships. Both formal leveraging strategies, with main focus on annotation precision, and heuristic alternatives, with main focus on scalability issues, have been described in literature. In this contribution, a factor graph approach to the hierarchical ensemble formulation of the automated GO annotation problem is presented. In this formal framework, a core factor graph is first built based on the GO structure and then enriched to take into account the noisy nature of GO-term predictions. Hence, starting from raw GO-term predictions, an iterative message passing algorithm between nodes of the factor graph is used to compute marginal probabilities of target GO-terms. Evaluations on Saccharomyces cerevisiae, Arabidopsis thaliana and Drosophila melanogaster protein sequences from the GO Molecular Function domain showed significant improvements over competing approaches, even when protein sequences were naively characterized by their physicochemical and secondary structure properties or when loose noisy annotation datasets were considered. Based on these promising results and using Arabidopsis thaliana annotation data, we extend our approach to the identification of most promising molecular function annotations for a set of proteins of unknown function in Solanum lycopersicum.","palabras_clave":"GO annotation|Factor Graph|Hierarchical Classifiers"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5571,"idioma_id":4,"titulo":"A high temperature study on the structure, linear expansion, thermodynamic stability and electrical properties of the BaCe0.8Pr0.2O3-&#948; perovskite","resumen":"Single phase BaCe0.8Pr0.2O3&#8722;&#948; perovkite was obtained by the modified Pechini method followed by self-combustion and sintering at 1350&#9702;C. Crystal structure, thermodynamic stability, linear expansion and electrical properties were characterized as a function of temperature, under oxidizing and reducing atmospheres. BaCe0.8Pr0.2O3&#8722;&#948; presents phase transitions under both atmospheres, which do not compromise the transport properties. The electrical conductivity showed a mixed character, dominated by electronic and oxygen vacancies transport in O2-containing atmosphere. Pr+4 reduction was observed above 400&#9702;C under H2-containing atmosphere. This reduction increases the oxygen non-stoichiometry and OH? charge carriers concentration, unit cell volume and thermal expansion coefficients (TECs). Pr+4 reduction suppresses the electronic conduction and hence the transport number for OH? would be dominant in wet reducing atmosphere.","palabras_clave":"BaCeO3|Proton conductor|SOFC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5572,"idioma_id":4,"titulo":"Preparation and characterization of poly  (vinylalcohol) \/ bentonite hydrogels for potential  wound dressings .","resumen":"During the last decade researchers have been working to find effective wound dressing materials. The materials have to be designed to hold moisture in the surface of the wound, providing the ideal environment for cleaning the wound, absorbing the exudates, eliminating the odour and promoting the healing process. In this scenario, hydrogels emerge as excellent options for that. However, due to poor mechanical and antimicrobial properties of many conventional hydrogels, composite hydrogels are now designed in order to improve mechanical stiffness and durability. For this purpose, nanocomposite based on poly(vinyl alcohol) (PVOH) and different concentration of bentonite (0-7 wt.%) were obtained by the freezing-thawing technique and characterized by means of morphological, physical, thermal, mechanical, barrier and antimicrobial properties. Herein it was developed a non-expensive, eco-friendly and a facile method to obtain nanocomposite hydrogels based on PVOH with reasonable mechanical properties (Young Modulus of 0.5-0.8 MPa), good microbial barrier properties, adequate water vapour transmission rates and excellent swelling behaviour (195-336%). Moreover, it was found that the porous sizes of the samples can be controlled by the addition of the clay. All obtained results indicate that the PVOH\/ 3% bentonite hydrogels show potential to be used as wound healing.","palabras_clave":"Wound dressing|Nanocomposites|Composite hydrogel|Bentonite"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5573,"idioma_id":4,"titulo":"Antilisterial peptides from Spanish dry-cured hams: Purification and identification","resumen":"The typical Spanish dry-cured ham has a particular sensory quality that makes it a distinctive food, highly appreciated for consumers worldwide. Its particular physicochemical properties, such as high salt content and reduced water activity contribute to their shelf-stability. However, post-processing actionscarried out for the commercialization of these products such as slicing may increase the risk of development of pathogenic microorganisms as Listeria monocytogenes. During ripening, muscle proteins are hydrolyzed by muscle peptidases releasing peptides and free amino acids. Some of these peptides havebeen described to exert biological activities such as antioxidant and ACE-inhibition. In this study, a peptidomic strategy using mass spectrometry techniques has been used to identify and sequence those naturally generated peptides showing antilisterial activity. One hundred and five peptides have beenidentified in active fractions and some synthesized and their MIC calculated. Ten peptides were able to inhibit the growth of L. monocytogenes, being the pentapeptide RHGYM the most effective showing a MIC value of 6.25 mM. This study proves for the first time the potential antimicrobial action againstL. monocytogenes of certain naturally generated peptides obtained from Spanish dry-cured ham.","palabras_clave":"antilisterial peptides|dry-cured ham|Lactic acid bacteria"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5574,"idioma_id":4,"titulo":"The MUSCLES Treasury Survey. I. Motivation and Overview","resumen":"Ground- and space-based planet searches employing radial velocity techniques and transit photometry have detectedthousands of planet-hosting stars in the Milky Way. With so many planets discovered, the next step towardidentifying potentially habitable planets is atmospheric characterization. While the Sun?Earth system provides a goodframework for understanding the atmospheric chemistry of Earth-like planets around solar-type stars, theobservational and theoretical constraints on the atmospheres of rocky planets in the habitable zones (HZs) aroundlow-mass stars (K and M dwarfs) are relatively few. The chemistry of these atmospheres is controlled by the shapeand absolute flux of the stellar spectral energy distribution (SED), however, flux distributions of relatively inactivelow-mass stars are poorly understood at present. To address this issue, we have executed a panchromatic (X-ray tomid-IR) study of the SEDs of 11 nearby planet-hosting stars, the Measurements of the Ultraviolet SpectralCharacteristics of Low-mass Exoplanetary Systems (MUSCLES) Treasury Survey. The MUSCLES program consistsvisible observations from Hubble and ground-based observatories. Infrared and astrophysically inaccessiblewavelengths (EUV and Ly&#945;) are reconstructed using stellar model spectra to fill in gaps in the observational data. Inthis overview and the companion papers describing the MUSCLES survey, we show that energetic radiation (X-rayand ultraviolet) is present from magnetically active stellar atmospheres at all times for stars as late as M6. Theemission line luminosities of C IV and Mg II are strongly correlated with band-integrated luminosities and we presentempirical relations that can be used to estimate broadband FUV and XUV (&#8801;X-ray + EUV) fluxes from individualstellar emission line measurements. We find that while the slope of the SED, FUV\/NUV, increases by approximatelytwo orders of magnitude form early K to late M dwarfs (&#8776;0.01?1), the absolute FUV and XUV flux levels at theircorresponding HZ distances are constant to within factors of a few, spanning the range 10?70 erg cm&#8722;2 s&#8722;1 in the HZ.Despite the lack of strong stellar activity indicators in their optical spectra, several of the M dwarfs in our sampleshow spectacular UV flare emission in their light curves. We present an example with flare\/quiescent ultraviolet fluxratios of the order of 100:1 where the transition region energy output during the flare is comparable to the totalquiescent luminosity of the star Eflare(UV) &#8764; 0.3 L*&#916;t (&#916;t = 1 s). Finally, we interpret enhanced L(line)\/LBol ratiosfor C IV and N V as tentative observational evidence for the interaction of planets with large planetary mass-to-orbitaldistance ratios (Mplan\/aplan) with the transition regions of their host stars.","palabras_clave":"PLANETARY SYSTEMS|ACTIVITY|LOW MASS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5575,"idioma_id":8,"titulo":"Virus evolution during chronic hepatitis B virus infection as revealed by ultradeep sequencing data","resumen":"Despite chronic hepatitis B virus (HBV) infection (CHB) being a leading cause of liver cirrhosis and cancer, HBV evolution during CHB is not fully understood. Recent studies have indicated that virus diversity progressively increases along the course of CHB and that some virus mutations correlate with severe liver conditions such as chronic hepatitis, cirrhosis and hepatocellular carcinoma. Using ultra-deep sequencing (UDS) data from an intrafamilial case, we detected such mutations at low frequencies among three immunotolerant patients and at high frequencies in an inactive carrier. Furthermore, our analyses indicated that the HBV population from the seroconverter patient underwent many genetic changes in response to virus clearance.Together, these data indicate a potential use of UDS for developing non-invasive biomarkers for monitoring disease changes over time or in response to specific therapies. In addition, our analyses revealed that virus clearance seemed not to require the virus effective population size to decline. A detailed genetic analysis of the viral lineages arising during and after the clearance suggested that mutations at or close to critical elements of the core promoter (enhancer II,epsilon encapsidation signal, TA2, TA3 and direct repeat 1-hormone response element) might be responsible for a sustained replication. This hypothesis requires the decline in virus load to be explained by constant clearance of virus-producing hepatocytes, consistent with the sustained progress towards serious liver conditions experienced by many CHB patients.","palabras_clave":"hepatitis B virus |evolución viral|Ultradeep sequencing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5576,"idioma_id":4,"titulo":"Staphylococcus aureus Induces Shedding of IL-1RII in Monocytes and Neutrophils","resumen":"Interleukin 1 (IL-1) &#946; is a critical cytokine that orchestrates host defenses against Staphylococcus aureus and is crucial for the eradication of bacteria. The production and action of IL-1&#946; are regulated by multiple control pathways. Among them, IL-1RII (the type II IL-1 receptor) acts as a decoy receptor and has been shown to regulate the biological effects of IL-1&#946;. High levels of soluble IL-1RII are present in septic patients; however, the stimuli that regulate the expression and release of IL-1RII in pathological conditions are incompletely elucidated. In the present study, we demonstrated the ability of S. aureus and protein A to induce IL-1RII shedding in myeloid cells. The positive modulation of IL-1RII expression and cleavage was associated with the failure to detect IL-1&#946; in response to S. aureus both in vitro and in vivo, suggesting that the soluble form of the receptor could be masking the availability of IL-1&#946;. The absence of detectable IL-1&#946; was associated with low levels of inflammatory cytokines and chemokines known to be regulated by IL-1&#946; and with increased bacterial persistence. Modulation of decoy receptors during systemic S. aureus infection is proposed as a new strategy used by this bacterium to evade the immune response","palabras_clave":"STAPHYLOCOCCUS AUREUS|NEUTROPHILS|MONOCYTES|INTERLEUKIN 1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5577,"idioma_id":4,"titulo":"Nest survival and predation in blue-fronted parrots: effects of nest initiation, stage of the nesting cycle, and cavity characteristics","resumen":"In this work we identify factors affecting nest survival in Blue-fronted Parrots breeding in the Chaco region of northern Argentina. We combined temporal and environmental variables in order to determine which best explain variation in (a) nest survival and (b) nest survival in relation to predation exclusively. We predicted that high and deep cavities (inaccessible for snakes and other predators) would have high daily survival rates. We compared the relative effects on nest survival of: (1) time of the breeding season, (2) stage of the nesting cycle, and (3) different characteristics of the nest cavity. We modeled daily survival rate for all observed nests (DSR), and daily survival rate for only nests that had been predated (daily survival rate in relation to predation exclusively, DSpR). Nest predation accounted for 50% of nest failures. DSR was on average 0.989, translating into an apparent hatching success of 0.39. DSR decreased with time of breeding and increased with nest age. DSpR decreased with time of breeding and inclination of the nest cavity entrance hole and increased with height from the ground of the entrance hole. There was a quadratic effect of nest age on DSpR, such that daily survival was highest during incubation, lowest at hatching and the early nestling stage but increasing throughout the remainder of the nestling stage. This effect became more pronounced as the breeding season advanced. Year and observer effects did not affect the probability of survival. Temporal patterns and characteristics of the nest cavity explained most of the variation in nest survival in Blue-fronted Parrots, which highlights the relevance of including these covariates in future studies of nest survival in parrots, and has important management and conservation implications.","palabras_clave":"Amazona aestiva|MARK|Nest survival|Blue-fronted Parrot"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5578,"idioma_id":4,"titulo":"New flow injection method for quality control of dietary supplements containing L-carnitine using extraction mediated by sodium taurodeoxycholate coacervate coupled to molecular fluorescence","resumen":"Carnitine (CAR) is an essential component of the tissue of animals, higher plants and many microorganisms; it plays an important role during the metabolism of the human body. CAR transfers fatty acids to the place of burning-mitochondria and aids in the transformation of fats into energy and in this way supports overweight reduction and immediate physical performance, increases resistance from physical load and protects heart from overload. In this study, a new methodology for the determination of L-CAR by molecular fluorescence is described, after derivatization with a fluorescence active substance, 9-fluorenylmethyl chloroformate. An on-line coacervation process was used in order to isolate the analyte of the complex matrix. The developed methodology was successfully applied to the control of carnitine content in dietary supplements. The detection limit and quantification obtained were 2.4 × 10&#8722; 8 mol L&#8722; 1 and 7.30 × 10&#8722; 8 mol L&#8722; 1; respectively. Recoveries between 82 and 103% were obtained. At optimized working conditions, the sample throughput was 30 samples h&#8722; 1 (without considering the required time for L-CAR derivatization). Thus short analysis and very low running costs compared with the LC\/MS conventional methodology can be cited as the main advantages of the on-line coacervation with fluorescence detection proposed methodology.","palabras_clave":"FLOW INJECTION ANALYSIS|FLUORESCENCE DETECTION|BILE SALT COACERVATION|L-CARNITINE|PHARMACEUTICAL QUALITY CONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5579,"idioma_id":4,"titulo":"THE ISOKINETIC BEHAVIOR IN DIFFUSION CONTROLLED GROWTH PROCESSES","resumen":"The isokinetic behaviour of crystallization processes controlled by diffusion is analysed under both isothermal and continuous cooling\/heating conditions. The kinetic function is computed employing an approximate expression for the so called exponential integral. Diffusion controlled 3D growth of homogeneously nucleating crystalline grains is an isokinetic reaction if the nucleation and diffusion activation energies are identical. In the no isokinetic range, the kinetic functions in both continuous heating and isothermal transformations are the same except for a factor which depends on the nucleation and diffusion activation energies rate. This development is applied to the crystallization of AgGeSe glasses. The primary crystallization kinetic of glasses with compositions (Ge 25 Se 75 ) 100-y Ag y (with y=10, 15, 20 and 25 at. %) was studied in a previous work using differential scanning calorimetry and X-ray diffraction. The analysis is grounded on the Kolmogorov-Johnson- Mehl-Avrami model generalized to account for the compositional changes of the parent phase, responsible for the decreasing of both the nucleation frequency and the growth rate of the primary grains. The kinetic study of the crystallization process from continuous heating calorimetric data has been performed applying the master curve method. The primary crystallization product is the ternary phase g-Ag 8 GeSe 6 . The values of apparent activation energy Ea , obtained are in the range: 2.0 eV< E a < 2.6 eV\/at. A model of diffusion controlled 3D growth with decreasing homogeneous nucleation and soft impingement has been developed that reproduces the rate of transformation obtained experimentally for the studied alloys.","palabras_clave":"Calorimetría|Difusión|Cinetica de cristalizacion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5581,"idioma_id":4,"titulo":". Immunological findings associated with Argentinean strains of Mycobacterium avium subsp. paratuberculosis in bovine models.","resumen":"tMycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of ruminant paratubercu-losis. The aim of this study was to evaluate the biological behavior of different Argentinean strains of MAPin two bovine infection models: macrophage (in vitro) and calf (in vivo) through the evaluation of earlyimmune responses at the peripheral and local levels. Two MAP strains (A and C) were selected taking intoaccount the different patterns of TNF- and IL-10 secretion displayed by infected bovine macrophagesin vitro. Two groups of calves were infected with 250 mg of total wet weight live MAP: strain A infectedgroup (MA, n = 3), strain C infected group (MC, n = 2). Another group of animals was mock-infected (MI,n = 3). Infection was confirmed by MAP culture of feces and microscopic observation of granulomatouslesions in the gut tissue. All infected calves showed positive results in the DTH skin test. A significantincrease in peripheral CD4CD25+cells in MC group on day 150 was detected. The specific cellular immuneresponse developed allowed the identification of the infection as early as 30 days in the MA group. How-ever, the percentage of CD8CD25+cells was significantly increased on day 120 in MC group. Significantdifferences between groups in proliferation and cellular responses were also detected in ileocecal lymphnode samples. In summary, the strains of MAP employed herein induced differential immune responsesin peripheral cells, in the proliferative responses and in cell functionality at the local level. Our findingssupport the hypotheses that the in vitro behavior displayed by macrophages could be a tool to identifydifferences among MAP strains infecting bovines and that the host-pathogen interactions occurring uponinfection are dependent on the strain of MAP involved.","palabras_clave":"Immunological |paratuberculosis|calves|argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5582,"idioma_id":5,"titulo":"Análisis de la preservación ósea y dentaria en dos grupos etarios. Su importancia en el estudio de conjuntos esqueléticos","resumen":"Se presentan aquí los resultados del análisis de preservación de restos óseos y dentarios humanos de individuos adultos y subadultos provenientes de un cementerio contemporáneo.La representatividad, completitud y preservación de los elementos óseos y dentarios son fundamentales en estudios bioantropológicos, dado que dichos atributos posibilitan ladefinición de estrategias de investigación que conduzcan a interpretaciones demográficas y sociales. La condición y composición de los conjuntos osteológicos debe ser analizada teniendo en cuenta la relación entre factores intrínsecos y extrínsecos o tafonómicos que afectan su preservación. Para el análisis de la preservación, se tuvieron en cuenta tres dimensiones: la representación ósea y dentaria, el grado de fragmentación y el grado de deterioro y completitud del hueso. Para ello, se utilizaron el Índice de Representación Ósea (IRO), el Índice de Representación Dentaria (IRD), el Índice de Fragmentación Ósea (IFO), el Índice de Preservación Anatómica (IPA) y el Índice de Preservación del Hueso Cortical (IPC). Los resultados obtenidos mostraron diferencias significativas en la preservación entre adultos lo que se explicaría por diferencias en las características de los tejidos esqueléticos en diferentes momentos del desarrollo.","palabras_clave":"COLECCION OSTEOLOGICA |PRESERVACION |ADULTO|SUBADULTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5583,"idioma_id":4,"titulo":"The role of halogen C-X1---X2-C contact on the preferred conformation of 2-perhalomethylchromones in solid state","resumen":"The solid state of 2-chlorodifluoromethyl-3-methylchromone (1) and 3-bromomethyl-2-chlorodifluoromethylchromone (2) was studied by vibrational spectroscopy (IR, Raman) and X-ray diffraction methods. The analysis was extended to solution phase using UV?Vis, NMR ( 1 H, 13 C and 19 F) and fluorescence spectroscopy with the assistance of theoretical calculations. The crystal structure of 2 shows chlorine and bromine atoms of the ?CF 2 Cl and ?CH 2 Br groups on the same side of the molecular plane, while in the most stable conformer predicted by theoretical calculations, these halogen atoms are opposite. These differences were interpreted in terms of intra- and intermolecular interactions in the crystal packing. Particularly, the weak non-covalent halogen?halogen contact plays a decisive role in the preferred conformation adopted in solid phase. In addition, results of fluorescence spectroscopy are in accordance with the X-ray spectroscopy, since the proposed association due to intermolecular interactions was also found in solution when the concentration of 2 was increased.","palabras_clave":"2-Chlorodifluoromethylchromones|Halogenhalogen contacts|Single crystal X-ray diffraction|Spectroscopic properties"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5584,"idioma_id":4,"titulo":"Novel biopreservatives to enhance the safety and quality of strawberry juice","resumen":"Abstract Pomegranate extract, vanillin and geraniol werestudied as natural antimicrobials on strawberry juice.Strawberry juice was treated with each agent at two concentrations:pomegranate extract at 180 and 360 &#956;g\/mL; vanillinat 2.5 and 5 mg\/mL; and geraniol at 0.6 and 1.2 &#956;L\/mL. Afterbeing treated, juices were stored at 5 °C and microbiological,physicochemical and sensory studies were carried out. Also, asecond batch of juice was inoculated with Escherichia coliO157:H7 (105 CFU\/mL) before being treated, to safety study.Geraniol and vanillin, at both concentrations tested, werehighly effective in reducing the native microflora on strawberryjuice (more than 3 log cycles), extending the microbiologicalshelf-life of the product. Moreover, both antimicrobialsimproved the product safety by reducing inoculated E. coliO157:H7. Furthermore, vanillin showed a significant increasein polyphenol content compared to untreated juice. On theother hand, pomegranate extract applied at the highest concentrationshowed important reductions on mesophilic andpsychrophilic bacteria, but no effect on yeast and molds andinoculated E. coli. Even though vanillin and geraniol incorporationon strawberry juice had a negative effect on its sensoryquality, pomegranate extract had no impact on the sensoryattributes evaluated. Combinations of the biopreservativescould be studied in order to decrease the concentration of theantimicrobials, reducing the effects on strawberry juice sensorycharacteristics.","palabras_clave":"Pomegranate extract, Vanillin . Geraniol|Escherichia coli O157:H7|Antioxidant capacity |Native microflora ."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5585,"idioma_id":4,"titulo":"Molecular tracking of individual host use in the Shiny Cowbird, a generalist brood parasite","resumen":"Generalist parasites exploit multiple host species at the population level, but the individual parasite?s strategy may be either itself a generalist or a specialist pattern of host species use. Here, we studied the relationship between host availability and host use in the individual parasitism patterns of the Shiny Cowbird Molothrus bonariensis, a generalist avian obligate brood parasite that parasitizes an extreme range of hosts. Using five microsatellite markers and an 1120-bp fragment of the mtDNA control region, we reconstructed full-sibling groups from 359 cowbird eggs and chicks found in nests of the two most frequent hosts in our study area, the Chalk-browed Mockingbird Mimus saturninus and the House Wren Troglodytes aedon. We were able to infer the laying behavior of 17 different females a posteriori and found that they were mostly faithful to a particular laying area and host species along the entire reproductive season and did not avoid using previously parasitized nests (multiple parasitism) even when other nests were available for parasitism. Moreover, we found females using the same host nest more than once (repeated parasitism), which had not been previously reported for this species. We also found few females parasitizing more than one host species. The use of an alternative host was not related to the main hosts? nest availability. Overall, female shiny cowbirds use a spatially structured and host species specific approach for parasitism, but they do so nonexclusively, resulting in both detectable levels of multiple parasitism and generalism at the level of individual parasites.","palabras_clave":"host preference|molecular markers|Molothrus bonariensis|nest-use strategies"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5586,"idioma_id":4,"titulo":"Vascular plants of Sierra de Famatina (La Rioja, Argentina): an analysis of its biodiversity","resumen":"The Sierra de Famatina System is one of the main areas of endemism within the Southern Cone, as well as one of the main diversity hotspots of Argentina. We present here the first checklist for Sierra de Famatina, which includes 909 taxa (species, subspecies, varieties and forms) of vascular plants from 77 families and 358 genera. Sixty-eight are new records for La Rioja province and 28 are endemic species. Voucher specimens, locality information and distribution are given for each taxon, and a brief analysis of the diversity, phytogeography of the flora and the type specimens is presented. Illustrations of the important endemics and type specimens from Sierra de Famatina are provided. Our results emphasize the necessity to give high priority to the Sierra de Famatina region in terms of the conservation of its flora.","palabras_clave":"Central Argentina|botanical exploration|high mountains|endemisms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5587,"idioma_id":4,"titulo":"Self-organization of gliadin in aqueous media under physiological digestive pHs","resumen":"Here we showed that gliadin, a complex protein system related to celiac disease and other human dis-eases, is spontaneously self-organized in a very dilute solution at pH 3.0 and 7.0 in water under low ionicstrength (10 mM NaCl). The spontaneous self-organization at pH 3.0 increases the apparent solubilitydue to the formation of finite sized aggregates, such as those formed in the micellization of amphiphilicmolecules. Switching the pH from 3.0 to 7.0 lead to a phase separation, however part of the nano-particlesare stable remaining disperse in water after centrifugation. Also, beside the pH change led to changesin protein composition and concentration, we determined that the secondary structure of both systemis the same. Moreover, Tyrs are slightly more buried and Trps are slightly more exposed to water at pH7.0 than those at pH 3.0. Electron microscopy techniques showed that both gliadin systems are com-posed of nanostructures and in the case of pH 7.0 amorphous microaggregates were found, too. Onlynanostructures at pH 3.0 showed a micromolar binding affinity to Nile red probe, suggesting the pres-ence of accessible hydrophobic patches which are not more accessible at pH 7.0. All our results suggestthat gliadin is able to self-organized at pH 3.0 forming protein micelles type nanostructures ( = + 13,42 ± 1.55 mV), meanwhile at 7.0 the decrease of superficial charge to  of +4, 78 ± 0.48 mV led to theformation of stable colloidal nanoparticles, unable to interact with Nile red probe. Our findings may opennew perspectives for the understanding of gliadin ability to avoid proteolysis, to reach and cross theintestinal lumen and to trigger different immunological disorders.","palabras_clave":"Gliadin nanostructures|Electron microscopy|Gliadin related disorders"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5588,"idioma_id":5,"titulo":"Novedades para la flora de la Argentina","resumen":"Con motivo de estar preparando la Flora de Córdoba, han surgido numerosas novedades para la flora argentina, a raíz de haberse intensificado los viajes de campo y la revisión de las colecciones de los principales herbarios del país. En este trabajo se dan a conocer tres nuevas citas para la flora nativa y 17 para la flora introducida de la Argentina (especies casuales y naturalizadas). Se describen e ilustran los nuevos registros y se realiza una lectotipificación. Además, se documenta formalmente por primera vez la presencia de 10 especies introducidas.","palabras_clave":"nuevos registros|flora introducida|flora nativa|lectotipificación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5589,"idioma_id":4,"titulo":"Freezing resistance in Patagonian woody shrubs: the role of cell wall elasticity and stem vessel size","resumen":"Freezing resistance through avoidance or tolerance of extracellular ice nucleation is important for plant survival in habitats withfrequent subzero temperatures. However, the role of cell walls in leaf freezing resistance and the coordination between leaf andstem physiological processes under subzero temperatures are not well understood. We studied leaf and stem responses to freezingtemperatures, leaf and stem supercooling, leaf bulk elastic modulus and stem xylem vessel size of six Patagonian shrubspecies from two sites (plateau and low elevation sites) with different elevation and minimum temperatures. Ice seeding wasinitiated in the stem and quickly spread to leaves, but two species from the plateau site had barriers against rapid spread of ice.Shrubs with xylem vessels smaller in diameter had greater stem supercooling capacity, i.e., ice nucleated at lower subzero temperatures.Only one species with the lowest ice nucleation temperature among all species studied exhibited freezing avoidanceby substantial supercooling, while the rest were able to tolerate extracellular freezing from &#8722;11.3 to &#8722;20 °C. Leaves of specieswith more rigid cell walls (higher bulk elastic modulus) could survive freezing to lower subzero temperatures, suggesting thatrigid cell walls potentially reduce the degree of physical injury to cell membranes during the extracellular freezing and\/or thawprocesses. In conclusion, our results reveal the temporal?spatial ice spreading pattern (from stem to leaves) in Patagonian shrubs,and indicate the role of xylem vessel size in determining supercooling capacity and the role of cell wall elasticity in determiningleaf tolerance of extracellular ice formation.","palabras_clave":"COLD RESISTANCE|STEPPE|ICE NUCLEATION TEMPERATURE|LETHAL DAMAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5590,"idioma_id":4,"titulo":"Remote sensing and open top chamber field measurements of seasonal patterns of evapotranspiration in an extreme cold Patagonian desert","resumen":"Accurate estimates of seasonal evapotranspiration (ET) at different temporal and spatial scales are essential for understanding the biological and environmental determinants of ecosystem water balance in arid regions and the patterns of water utilization by the vegetation. For this purpose, remote sensing ET estimates of a Patagonian desert in Southern Argentina were verified with field measurements of soil evaporation and plant transpiration using an open top chamber. Root distribution and seasonal variation in soil volumetric water content was also analyzed. There was a high correlation between remote sensing and field measurements of ecosystem water fluxes. A substantial amount of the annual ET occurred in spring and early summer (73.4 mm) using winter rain stored in the soil profile and resulting in water content depletion of the upper soil layers. A smaller amount of annual ET was derived from few rainfall events occurring during the mid or late summer (41.4 mm). According to remote sensing, the 92.9 % of the mean annual precipitation returns to the atmosphere by transpiration or evaporation from the bare soil and by canopy interception. Only 7.1 % infiltrates to soil layers deeper than 200 cm contributing to the water table recharge. Fourier time series analysis, cross-correlation methods and multiple linear regression models were used to analyze 11 years of remote sensing data to assess determinants of water fluxes. A linear model predicts well the variables that drive complex ecosystem processes such as ET. Leaf area index and air temperature were not linearly correlated to ET because of the multiple interaction among variables resulting in time lags with ET variations and thus these two variables were not included in the linear model. Soil water content, the fraction of photosynthetic active radiation and precipitation explained 86% of the ET monthly variations. The high volumetric water content and the small seasonal variations at 200 cm depth were probably the result of little water uptake from deeper soil horizons by roots with low hydraulic conductivity.","palabras_clave":"MODIS|precipitation pulses|root distribution|soil water content"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5591,"idioma_id":4,"titulo":"Modulation of the default-mode network and the attentional network by self-referential processes in patients with disorder of consciousness","resumen":"Disorders of consciousness (DOC) are related to an altered capacity of the brain to successfully integrate and segregate information. Alterations in brain functional networks structure has been found in fMRI studies, which could account for the impossibility of the brain to efficiently manage internally and externally generated information. Here we assess the modulation of neural activity in areas of the networks related to active introspective or extrospective processing in patients with DOC and controls with fMRI. In addition, we assess the functional connectivity between those areas in resting state. General modulation of the brain in response to the task improves with the level of consciousness, denoting an integral recovery of the brain to be engaged in cognitive processes. In addition, functional connectivity is altered within and between the Default Mode and Attentional networks in opposite directions. Our results help to further understand the neural underpins of disorders of consciousness.","palabras_clave":"CONSCIOUSNESS DISORDERS|FUNCTIONAL MAGNETIC RESONANCE IMAGING|FUNCTIONAL CONNECTIVITY|SELF REFLECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5592,"idioma_id":4,"titulo":"Phylogeography of Cnesterodon decemmaculatus (Cyprinodontiformes: poeciilidae) in Southern Pampas, Argentina: ancient versus recent patterns in freshwater fishes","resumen":"The Southern Pampean Area includes the southernmost limit of the Brazilian subregion and represents an interesting model to study how evolution of the landscape has affected the distribution of freshwater fishes in time and space. The hydrogeographic pattern of the region results from a combination of factors including climatic and sea level changes occurred since the Miocene and extending into the Quaternary. The hydrologic basins of this region are isolated, and have a similar ichthyological composition among them. Here we use a phylogeographic approach to infer how paleoclimatic changes affected the distribution of genetic variation in the populations of freshwater fishes. We analyzed the control region of mtDNA of Cnesterodon decemmaculatus and compared it with published data for the species Jenynsia multidentata and Corydoras paleatus in the same area. Cnesterodon decemmaculatus showed two main haplotypes with scarce divergence and wide geographical distribution within the area. The low divergence found between the Cnesterodon decemmaculatus haplotypes and the results obtained in the demographic analyses could suggest that the presence of this species in the area have a recent demographic history. These results are in contrast with the pattern reported for Jenynsia multidentata and Corydoras paleatus, where data suggest an ancient history in the area. The incongruence in the observed phylogeographical patterns could be due to the different ecological requirements of each species, and to the various responses of them to the environmental conditions resulting from geologic and paleoclimatic changes occurred in the region during the Late Quaternary.","palabras_clave":"BRAZILIAN SUBREGION|COMPARATIVE PHYLOGEOGRAPHY|DEMOGRAPHIC HISTORY|SOUTHERN PAMPEAN AREA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5593,"idioma_id":4,"titulo":"Evaluation of serological response to foot-and-mouth disease vaccination in BLV infected cows","resumen":"Background: Bovine Leukemia Virus (BLV) produces disorders on the immune system in naturally infected animals,which may counteract the development of immunity after vaccination. The aim of this study was to investigatewhether healthy and BLV infected cattle elicited similar humoral responses after foot and mouth disease (FMD)immunization. In a field study, 35 Holstein heifers were selected based on their BLV serological status and immunizedwith a single dose of a commercial bivalent oil-based FMD vaccine. Serum samples were collected at 0, 15, 60, 165and 300 days post vaccination (dpv).Results: Total anti-A24\/Cruzeiro antibodies, IgM, IgG1, IgG2 titers and avidity index of specific antibodies weredetermined by ELISA. Although only marginally significant differences were found between groups in terms of totalantibodies, anti-FMD IgM and IgG1 titers were significantly lower in heifers infected with BLV at the 15 dpv (p < 0.01).Animals that became infected during the study did not show differences to the BLV negative group.Conclusions: Cattle infected with BLV at the time of immunization may elicit a low-magnitude serological response toa commercial Foot-and-mouth disease vaccine","palabras_clave":"RESPUESTA INMUNE|BLV|SEROLOGIA|VACUNA ANTI AFTOSA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5594,"idioma_id":4,"titulo":"Intrinsic Disorder to Order Transitions in the Scaffold Phosphoprotein P from the Respiratory Syncytial Virus RNA Polymerase Complex","resumen":"Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which consists of a four-helix bundle tetramerization domain flanked by two domains predicted to be intrinsically disordered. Because intrinsic disorder cannot be reduced to a defined atomic structure, we tackled the experimental dissection of the disorder&#8722;order transitions of P by a domain fragmentation approach. P remains as a tetramer above 70 °C but shows a pronounced reversible secondary structure transition between 10 and 60 °C. While the N-terminal module behaves as a random coil-like IDP in a manner independent of tetramerization, the isolated C-terminal module displays a cooperative and reversible metastable transition. When linked to the tetramerization domain, the C-terminal module becomes markedly more structured and stable, with strong ANS binding. Therefore, the tertiary structure in the C-terminal module is not compact, conferring ?late? molten globule-like IDP properties, stabilized by interactions favored by tetramerization. The presence of a folded structure highly sensitive to temperature, reversibly and almost instantly formed and broken, suggests a temperature sensing activity. The marginal stability allows for exposure of protein binding sites, offering a thermodynamic and kinetic fine-tuning in order&#8722;disorder transitions, essential for the assembly and function of the RSV RNA polymerase complex.","palabras_clave":"RESPIRATORY SINCYTIAL VIRUS|P PROTEIN|INTRINSIC DISORDER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5595,"idioma_id":4,"titulo":"'CtBP1 associates metabolic syndrome and breast carcinogenesis targeting multiple miRNAs'","resumen":"Metabolic syndrome (MeS) has been identified as a risk factor for breast cancer.C-terminal binding protein 1 (CtBP1) is a co-repressor of tumor suppressor genesthat is activated by low NAD+\/NADH ratio. High fat diet (HFD) increases intracellular NADH. We investigated the effect of CtBP1 hyperactivation by HFD intake on mouse breast carcinogenesis. We generated a MeS-like disease in female mice by chronically feeding animals with HFD. MeS increased postnatal mammary gland development and generated prominent duct patterns with markedly increased CtBP1 and Cyclin D1 expression. CtBP1 induced breast cancer cells proliferation. Serum from animals with MeS enriched the stem-like\/progenitor cell population from breast cancer cells. CtBP1 increased breast tumor growth in MeS mice modulating multiple genes and miRNA expression implicated in cell proliferation, progenitor cells phenotype, epithelial to mesenchymal transition, mammary development and cell communication in the xenografts. These results define a novel function for CtBP1 in breast carcinogenesis.","palabras_clave":"CtBP1|metabolic syndrome|high fat diet|breast cancer|miRNAs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5596,"idioma_id":4,"titulo":"Structural, physical and chemical properties of nanostructured nickel-substituted ceria oxides under reducing and oxidizing conditions","resumen":"Thiswork reports the synthesis of nanostructured Ce1-xNixO2-d(x = 0.05, 0.1, 0.15 and 0.2) oxides prepared bycation complexation route and with the main objective of studying their redoxproperties using a combination of electron microscopy, synchrotron radiationX-ray diffraction (SR-XRD) and X-ray absorption near-edge spectroscopy (XANES).The Ce1-xNixO2-d series of nanopowders maintain the cubiccrystal structure (Fm3m space group) of pure ceria, with an average crystallitesize of 5-7 nm indicated by XRD patterns and confirmed by transmission electronmicroscopy. In situ SR-XRD and XANEScarried out under reducing (5% H2\/He; 5% CO\/He) and oxidizing (21%O2\/N2)atmospheres at temperatures up to 500 °C show a Ni solubility limit close to 15at.% in air at room temperature, decreasing to about 10 at.% after exposure to5% H2\/He atmosphere at 500 °C. At room temperature in air, theeffect of Ni on the lattice parameter of Ce1-xNixO2-d is negligible, whereas a marked expansion ofthe lattice is observed at 500 °C in reducing conditions. This is shown byXANES to be correlated with the reduction of up to 25% of Ce4+cations to the much larger Ce3+, possibly accompanied by theformation of oxygen vacancies. The redox ability of the Ce4+\/Ce3+couple in nanocrystalline Ni-doped ceria is greatly enhanced in comparison topure ceria or achieved by using other dopants (e.g. Gd, Tb or Pr), where it islimited to lessthan5% of Ce cations.","palabras_clave":"Ceria|Metales de Transcición|XRD|XAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5597,"idioma_id":4,"titulo":"HDN of indole over Ir-modified Ti-SBA-15","resumen":"tThe hydrodenitrogenation of indole has been studied over a series of Ti-modified SBA-15 supports andtheir respective Ir-catalysts. Ti-SBA-15 was synthesized using sol-gel method and Ti was added duringthe synthesis using Tetraethyl Orthotitanate. Iridium was added by wetness impregnation. The acidityof Ti-SBA-15 was modified adding F or Al in order to improve the catalytic activity. The catalysts pre-pared were extensively characterized by X-ray diffraction (XRD), N2adsorption isotherms, UV?vis-DRS,Raman spectroscopy,27Al-MAS-NMR spectroscopy, XPS, TEM-EDS, Py-FTIR, H2-Chemisorption and TPR.UV?vis-DRS and Raman spectroscopy confirmed that Ti was incorporated in tetrahedral coordination inthe framework of the SBA-15. The analysis showed that the mesoporous structure was maintained afterF or Al incorporation.27Al-MAS-NMR spectroscopy indicated that aluminum was incorporated mainly inTd positions. The results showed that Ti incorporation helps to reduce significantly the size of iridiumcrystallites and improves its dispersion considerably. Ir\/Ti-SBA-15 modified with Al, with Bronsted acid-ity, was the most active catalyst for indole HDN, in mild conditions in a Batch reactor. The experimentaldata for indole hydrodenitrogenation was quantitatively represented by a Langmuir?Hinshelwood typerate equation","palabras_clave":"HYDRODENITROGENATION|IRIDIUM|TITANIUM|KINETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5598,"idioma_id":4,"titulo":"Optimization of the synthesis of SBA-3 mesoporous materials by experimental design","resumen":"SBA-3 mesoporous materials are characterized by hexagonal regular arrangements of channels with diameters >2 nm, high specific surface areas and high specific pore volumes. In the work reported herein, experimental design-response surface methodology (RSM) is used to model and optimize the synthesis conditions for SBA-3 mesoporous materials. In this study, we evaluate the influences of surfactant\/silica source molar ratios, aging times, temperature and pH on the synthesis of SBA-3 mesoporous materials by analyzing the XRD intensities pertaining to the [100] signal. Response surfaces were obtained using the BoxeBehnken design, and the combination of reaction parameters was optimized. By applying statistical methodology, higher levels of the objective function (XRD intensities pertaining to the [100] signal) were obtained using cetyltrimethylammonium bromide (CTAB)\/tetraethyl orthosilicate (TEOS) molar ratios of 0.07 and 0.16, HCl\/TEOS molar ratios of 8 and 11, reaction temperatures of 35 and 45  C and aging times of 12e24 h. The mesoporous SBA-3 samples obtained were characterized using small-angle X-ray powder diffraction (XRD), BET, FTIR and 29Si NMR-MAS, scanning electron microscopy (SEM) and transmission electron microscopy (TEM).","palabras_clave":"SBA-3|Synthesis|design-response surface|optimal synthesis conditions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5599,"idioma_id":4,"titulo":"Vanadium and titanium oxide supported on mesoporous CMK-3 as new catalysts for oxidative desulfurization","resumen":"Vanadium supported-CMK-3 catalysts with vanadium loading of 1-7 wt.% were studied in the oxidative desulfurization(ODS) of dibenzothiophene as a model sulfur compound. The activity was compared with titanium supported-CMK-3.Structural and textural characterization of the catalysts was performed by means of N2adsorption, XRD, UV-vis, DRS,Raman spectroscopy, XPS, TEM and TPR. The dispersion and the nature of the vanadium species depend on the V loading,so does the catalyst activity. Vanadium supported-CMK-3 with 7 wt.% of vanadium loading was the most activecatalyst for ODS of DBT using hydrogen peroxide (H2O2) as oxidant and acetonitrile as solvent. 100% of DBT elimination was attained at short time in mild conditions. Carbon ordered mesoporous CMK-3 with high surface area and high pore volume promotes a very good anchorage of metallic oxides in the carbons framework reaching high active sites distribution and more stable nanoclusters. The reusability of the catalyst indicates that V-CMK-3 is a potential catalyst forthe ODS of dibenzothiophene.","palabras_clave":"ODS|Ti-V supported CMK catalysts|Dibenzothiophene|Ultra low sulfur diesel|Deactivation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5600,"idioma_id":4,"titulo":"Sulfur elimination by oxidative desulfurization with titanium-modified SBA-16","resumen":"TiO2-modified mesoporous SBA-16 and titanium-substituted mesoporous SBA-16 were developed and tested in the oxidative desulfurization (ODS) of dibenzothiophene prevailing in liquid fuel. Pure TiO2 was used as reference. The titania-based catalysts were characterized by chemical analysis, XRD, EDX and TEM. The titanium state as tetrahedral (in Ti-SBA-16 sample) or octahedral (in TiO2\/SBA-16 sample) coordination surrounding in the silicate matrix was determined by XPS, UV?vis DRS, FTIR, Raman and XANES. We assessed the impact exerted on performance of different reaction variables, including (nature and amount of the active catalytic species, phase system, molar ratio of oxidant H2O2 and DBT, reaction temperature, nature of the substrate and reuse of catalysts). In addition, we carried out a kinetic study and the activation energy was determined. We achieved 90% of S removal from a 0.2&nbsp;wt.% dibenzothiophene solution at 60&nbsp;°C in less than 1&nbsp;h of reaction. The best catalytic results are obtained with high exposed surface of nanometric TiO2 species of TiO2\/SBA-16 sample. The activated catalyst is very active in ODS reaction and can be reused four times with no loss in activity.","palabras_clave":"ODS|Ti-SBA-16|TITANIA| Reaction Kinetics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5601,"idioma_id":4,"titulo":"Co-Rh modified natural zeolites as new catalytic materials to oxidize propane and naphthalene from emission sources","resumen":"Natural zeolites as a raw material to prepare catalytic precursors for the oxidation reaction of linear and poly-aromatic hydrocarbons are reported in this work. The process consisted in the formation of mono- and bi-metallic species containing Co and Co-Rh on natural zeolite tuffs. The materials are analyzed by different physicochemical techniques and used as catalysts for propane and naphthalene oxidation in emissions sources. Comparatively, Rh-zeolites are the most active catalysts for propane conversion. In this case, the formation of mixed oxides seems to be conditioned by surface properties. It could also be suggested that the Rh incorporation on a non-active phase in bimetallic catalysts impacts the effectiveness of the system. In addition, the NO presence increases the activity of bimetallic materials. Rh-Co zeolite systems markedly influence the naphthalene combustion temperature. Whereas in the absence of a catalyst a conversion rate of 50% and 100% is reached at 430 oC and 485 oC, respectively. It is interesting to observe that for RhCoCli-Mor and RhCoCli catalyst the 100% conversion is reached at 250 oC.","palabras_clave":"NATURAL ZEOLITES |COBALT|RHODIUM|HYDROCARBONS COMBUSTION|CATALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5602,"idioma_id":4,"titulo":"Experimental Design Optimization of the Tetralin Hydrogenation over Ir-Pt-SBA-15","resumen":"Experiment design-response surface methodology (RSM) is used in this work to modeland optimize two responses in the hydrogenation of tetralin to decalin using bimetallicIr-Pt-SBA-15 catalyst. In this study, we analyze the influence of the nature of thecatalyst (metal molar fraction and metal loading), the catalyst\/substrate ratio and thetemperature of the reactionas factors for the design. The responses analyzed wereconversion at 3 h and at 5 h of reaction time. The response surfaces were obtained withthe Box?Behnken Design, finding the best combination between the reaction parametersthat allowed optimizing the process. By applying the statistic methodology, the higherlevels of the two objective functions were obtained employing the catalyst with 1wt.%of iridium and 0.7-0.8wt.% of platinum; the optimal ratio between mass of catalyst andmole of tetralin was 17-19 and Temperature between 200-220°C.","palabras_clave":"hydrogenation|tetralin|experimental design|decalin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5603,"idioma_id":4,"titulo":"Synthesis and characterization of 2D-hexagonal, 3D-hexagonal and cubic mesoporous materials using CTAB and silica gel","resumen":"Highly ordered mesoporous materials SBA family (Santa Barbara Amorphous), with symmetry of two-dimensional (2D)-hexagonal SBA-3, three-dimensional (3D)-hexagonal P63\/mmc (SBA-7) and cubic Pm3n (SBA-1) were synthesized by a simple and easy procedure, designed using silica gel as a silicon source, CTAB (cetyltrimethylammonium bromide) as a structure-directing agent, in highly acidic conditions. The effect of the variation of temperature and reaction time allowed us to obtain the optimum condition to prepare SBA-1, SBA-3 and SBA-7materials. The sodiumsilicate generated ?in situ? by dissolution of the silica gel, at different concentrations of NaOH and the variation of the synthesis temperature and reaction time are the three factors which allow obtaining different SBA phases. Thus, the best SBA-3, SBA-1 and SBA-7 materials with well-ordered mesoporous systemcan be obtained in the range of 0.8, 0.9 and 1.0 NaOHconcentration and 30 and 25 °C and 45, 2880, 1440 min (for SBA-3, SBA-1 and SBA-7 respectively).","palabras_clave":"mesoporous|SBA-3|SBA-7|Silica gel"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5604,"idioma_id":4,"titulo":"Surface plasmons induced in Al spherical nanoparticles by Auger effect","resumen":"In this work we study the surface plasmon generation by electron-hole interaction in an aluminum sphericalnano-particle due to the Auger electron-hole interaction by means of the Hamiltonian formalism.","palabras_clave":"nanosphere|plasmon"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5605,"idioma_id":4,"titulo":"Meaningful traits for grouping plant species across arid ecosystems","resumen":"Grouping species may provide some degree of simplification to understand the ecological functionof plants on key ecosystem processes. We asked whether groups of plant species based on morpho-chemical traits associated with plant persistence and stress\/disturbance resistance reflect dominant plant growth forms in arid ecosystems. We selected twelve sites across an aridity gradient in northern Patagonia. At each site, we identified modal size plants of each dominant species and assessed specific leaf area (SLA), plant height, seed mass, N and soluble phenol concentration in green and senesced leaves at each plant. Plant species were grouped according with plant growth forms (perennial grasses, evergreen shrubs and deciduousshrubs) and plant morphological and\/or chemical traits using cluster analysis. We calculated mean values of each plant trait for each species group and plant growth form. Plant growth forms significantly differed among them in most of the morpho-chemical traits. Evergreen shrubs were tall plants with the highest seed mass and soluble phenols in leaves, deciduous shrubs were also tall plants with high SLA and the highest N in leaves, and perennial grasses were short plants with high SLA and low concentration of N and soluble phenols in leaves. Grouping species by the combination of morpho-chemical traits yielded 4 groups in which species from one growth form prevailed. These species groups differed in soluble phenol concentration in senesced leaves and plant height. These traits were highly correlated. We concluded that (1) plant height is a relevant synthetic variable, (2) growth forms adequately summarize ecological strategies of species in arid ecosystems, and (3) the inclusion of plant morphological and chemical traits related to defenses against environmental stresses and herbivory enhanced the potential of species grouping, particularly within shrubby growth forms","palabras_clave":"DESERT PLANTS |PLANT HEIGHT |SEED MASS |SOLUBLE PHENOLS |SPECIFIC LEAF AREA|N CONCENTRATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5606,"idioma_id":4,"titulo":"Silica core-shell particles for the dual delivery of gentamicin and rifamycin antibiotics.","resumen":"Increasing bacterial resistance calls for the simultaneous delivery of multiple antibiotics. One strategy is todesign a unique pharmaceutical carrier that is able to incorporate several drugs with different physicochemicalproperties. This is highly challenging as it may require the development of compartmentalizationapproaches. Here we have prepared core?shell silica particles allowing for the dual delivery of gentamicinand rifamycin. The effect of silica particle surface functionalization on antibiotic sorption was first studied,enlightening the role of electrostatic and hydrophobic interactions. This in turn dictates the chemicalconditions for shell deposition and further sorption of these antibiotics. In particular, the silica shelldeposition was favored by the positively charged layer of gentamicin coating on the core particlesurface. Shell modification by thiol groups finally allowed for rifamycin sorption. The antibacterial activityof the core?shell particles against Staphylococcus aureus and Pseudomonas aeruginosa demonstratedthe dual release and action of the two antibiotics.","palabras_clave":"drug delivery|antibiotics|Silica coreshell particles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5607,"idioma_id":4,"titulo":"Silver Nanoparticles: Biosynthesis Using an ATCC Reference Strain of Pseudomonas aeruginosa and Activity as Broad Spectrum Clinical Antibacterial Agents","resumen":"Currently, the biosynthesis of silver-based nanomaterials attracts enormous attention owing to the documented antimicrobial properties of these ones. This study reports the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using a Pseudomonas aeruginosa strain from a reference culture collection. A greenish culture supernatant of P. aeruginosa incubated at 37 °C with a silver nitrate solution for 24 h changed to a yellowish brown color, indicating the formation of Ag-NPs, which was confirmed by UV-vis spectroscopy, transmission electron microscopy, and X-ray diffraction. TEM analysis showed spherical and pseudospherical nanoparticles with a distributed size mainly between 25-45 nm, and the XRD pattern revealed the crystalline nature of Ag-NPs.  Also, it provides an evaluation of the antimicrobial activity of the biosynthesized Ag-NPs against human pathogenic and opportunistic microorganisms, namely, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Proteus mirabilis, Acinetobacter baumannii, Escherichia coli, P. aeruginosa, and Klebsiella pneumonia. Ag-NPs were found to be bioactive at picomolar concentration levels showing bactericidal effects against both Gram-positive and Gram-negative bacterial strains. This work demonstrates the first helpful use of biosynthesized Ag-NPs as broad spectrum bactericidal agents for clinical strains of pathogenic multidrug-resistant bacteria such as methicillin-resistant S. aureus, A. baumannii, and E. coli. In addition, these Ag-NPs showed negligible cytotoxic effect in human neutrophils suggesting low toxicity to the host.","palabras_clave":"SILVER NANOPARTICLES|BIOSYNTHESIS|ANTIBACTERIAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5608,"idioma_id":4,"titulo":"Synergistic Effect of Simple Sugars and Carboxymethyl Cellulose on the Production of a Cellulolytic Cocktail from Bacillus sp. AR03 and Enzyme Activity Characterization","resumen":"AbstractA cellulase-producing bacterium isolated from pulp and paper feedstock, Bacillus sp. AR03, was evaluated by means of a factorial design showing that peptone and carbohydrates were the main variables affecting enzyme production. Simple sugars, individually and combined with carboxymethyl cellulose (CMC), were further examined for their influence on cellulase production by strain AR03. Most of the mono and disaccharides assayed presented a synergistic effect with CMC. As a result, a peptone-based broth supplemented with 10 g\/L sucrose and 10 g\/L CMC maximized enzyme production after 96 h of cultivation. This medium was used to produce endoglucanases in a 1-L stirred tank reactor in batch mode at 30 °C, which reduced the fermentation period to 48 h and reaching 3.12&#8201;±&#8201;0.02 IU\/mL of enzyme activity. Bacillus sp. AR03 endoglucanases showed an optimum temperature of 60 °C and a pH of 6.0 with a wide range of pH stability. Furthermore, presence of 10 mM Mn2+ and 5 mM Co2+ produced an increase of enzyme activity (246.7 and 183.7 %, respectively), and remarkable tolerance to NaCl, Tween 80, and EDTA was also observed. According to our results, the properties of the cellulolytic cocktail from Bacillus sp. AR03 offer promising features in view of potential biorefinery applications.","palabras_clave":"BACILLUS SP.|FACTORIAL DESIGN|ENDOGLUCANASE PRODUCTION|ENZIME ACTIVITY|BIOREFINERY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5609,"idioma_id":4,"titulo":"Metabolic and behavioral responses of the reef fish Patagonotothen cornucola to ultraviolet radiation: Influence of the diet","resumen":"Most of the studies devoted to assess the effects of ultraviolet radiation (UVR) on fishes have been done with early life stages (i.e., eggs and larvae) as they are the most vulnerable to these wavelengths; however, the effects of UVR on juveniles or adults are less clear. This study evaluated the effects of UVR and diet (i.e., rich and poor in ultraviolet-absorbing compounds; UVAC) on the metabolic rates (respiratory frequency) and behavior (prey capture time) of juveniles of the reef fish Patagonotothen cornucola, a common species inhabiting the intertidal areas of the Patagonian coast. UVR was a signi ficant stress factor for P. cornucola by increasing its respiratory frequency, which could be related to the costs of repairing any cellular components damaged during the exposure. Fish exposed to UVR took more time to detect and capture their prey, which could lead to a reduction in the prey capture rates, with the concomitant effects on growth. In addition, juveniles feeding on rich-UVAC diet had a signi ficant lower respiratory frequency than those feeding on poor-UVAC diet. This differential response was not evident in the prey capture time. In their natural environment, P. cornucola could feed on a varied diet and could also partially avoid the exposure to UVR by hiding under the rocks or macroalgae. Future studies should consider the broad variety of diets that could be found in rocky intertidal areas, and how their qualities (in terms of UVAC content) could help to counteract the UVR effects on juvenile fish.","palabras_clave":"ULTRAVIOLET|FISH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5610,"idioma_id":4,"titulo":"-\tThe PKA regulatory subunit from yeast forms a homotetramer: low-resolution structure of the N-terminal oligomerization domain","resumen":"The cAMP dependent protein kinase (PKA) is a key enzyme involved in many cellular processes in eukaryotes. In mammals, the regulatory (R) subunit localizes the catalytic (C) subunit to specific subcellular sites through the interaction of its N-terminal homodimeric docking and dimerization (D\/D) domain with specific scaffold proteins. The structure of the D\/D domain has been extensively studied in mammals, but there is little information from non-mammalian species. In this work, we present the structural analysis of the D\/D domain of Bcy1, the R subunit of PKA from Saccharomyces cerevisiae. Using chemical crosslinking experiments and static light scattering measurements we found that this R subunit forms a tetramer in solution, unlike its dimeric mammalian counterparts. We determined that the D\/D domain is responsible for this unusual oligomeric state. Using biophysical techniques including size-exclusion chromatography, sucrose gradient sedimentation, small angle X-ray scattering (SAXS), and circular dichroism, we performed a detailed structural characterization of the tetrameric D\/D domain of Bcy1. We used homology modelling in combination with computer-aided docking methods and ab initio SAXS modelling methods to develop structural models for the D\/D domain tetramer. The models consist of two homodimers with a canonical D\/D domain fold that generate a dimer of dimers with novel putative interaction surfaces. These findings indicate that the oligomerization states of PKA R subunits is more diverse than previously thought, and suggest that this might allow some forms of PKA to interact with a wide range of intracellular partners.","palabras_clave":"PKA|Saccharomyces cerevisiae|BCY1|DD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5611,"idioma_id":5,"titulo":"Colectivos Inclusivos y Colectivos Reivindicativos. Propuesta conceptual para el abordaje de la cotidianeidad laboral","resumen":"El objetivo de este artículo es la presentación de las nociones de colectivos inclusivos y colectivos reivindicativos como herramientas para el abordaje de las relaciones propias de la cotidianeidad laboral ? entendida como el nivel de análisis distintivo del enfoque socioantropológico. Ambas nociones permiten entablar un diálogo crítico con tesis e investigaciones sobre el accionar obrero provenientes de diferentes campos disciplinares. En primer lugar, se presenta el proceso de delimitación empírica de los colectivos inclusivos y reivindicativos. En segundo término, se explicita el entramado teórico que sustenta las nociones propuestas. Finalmente, se desarrollan las categorías propuestas y se proponen hipótesis para el abordaje las prácticas de los trabajadores.","palabras_clave":"RELACIONES LABORALES|COTIDIANEIDAD|TRABAJADORES INDUSTRIALES|ACCIONAR SINDICAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5612,"idioma_id":4,"titulo":"A green method for the quantification of plastics-derived endocrine disruptors in beverages by chemometrics-assisted liquid chromatography with simultaneous diode array and fluorescent detection","resumen":"The aim of this study was to develop a novel analytical method for the determination of bisphenol A, nonylphenol, octylphenol, diethyl phthalate, dibutyl phthalate and diethylhexyl phthalate, compounds known for their endocrine-disruptor properties, based on liquid chromatography with simultaneous diode array and fluorescent detection. Following the principles of green analytical chemistry, solvent consumption and chromatographic run time were minimized. To deal with the resulting incomplete resolution in the chromatograms, a second-order calibration was proposed. Second-order data (elution time-absorbance wavelength and elution time-fluorescence emission wavelength matrices) were obtained and processed by multivariate curve resolution-alternating least-squares (MCR-ALS). Applying MCR-ALS allowed quantification of the analytes even in the presence of partially overlapped chromatographic and spectral bands among these compounds and the potential interferents. The obtained results from the analysis of beer, wine, soda, juice, water and distilled beverage samples were compared with gas chromatography-mass spectrometry (GC-MS). Limits of detection (LODs) in the range 0.04?0.38&nbsp;ng&nbsp;mL&#8722;1 were estimated in real samples after a very simple solid-phase extraction. All the samples were found to contain at least three EDs, in concentrations as high as 334&nbsp;ng&nbsp;mL&#8722;1.","palabras_clave":"ENDOCRINE DISRUPTORS    |LIQUID CHROMATOGRAPHY|SECOND-ORDER CALIBRATION|BEREVAGES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5613,"idioma_id":4,"titulo":"Synthesis and characterization of molecularly imprinted polymer nanoparticles for coenzyme Q10 dispersive micro solid phase extraction","resumen":"Molecularly imprinted polymer nanoparticles (MIPNPs) with the ability to recognize coenzyme Q10 (CoQ10) were synthesised in order to be employed as sorbent in a dispersive micro-solid phase extraction (DMSPE) for the determination of CoQ10 in a liver extract. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier, shuttling electrons from complex I (NADH-ubiquinone oxidoreductase) and II (succinate-ubiquinone oxidoreductase) to complex III (ubiquinol-cytochrome c reductase), for the production of cellular energy. The MIPNPs were synthesised by precipitation polymerization using coenzyme Q0 as the dummy template, methacrylic acid as the functional monomer, an acetonitrile: water mixture as the porogen, ethylene glycol dimethacrylate as the crosslinker and potassium persulfate as initiator. The nanoparticles were characterized by microscopy, capillary electrophoresis, dynamic light scattering, N2 adsorption?desorption isotherms, and infrared spectroscopy. The MIPNPs demonstrated the presence of selective cavities complementary to the quinone nucleus of CoQ10, leading to a specific recognition of CoQ10 compared with related compounds. In the liver extract the relative CoQ10 peak area (CoQ10 area\/total peak area) increased from 4.6% to 25.4% after the DMSPE procedure. The recovery percentage of CoQ10 from the liver matrix was between 70.5% and 83.7% quantified against CoQ10 standard processed under the same conditions. The DMSPE procedure allows the elution of almost all the CoQ10 retained (99.4%) in a small volume (200 &#956;L), allowing the sample to be concentrated 2.5 times (LOD: 1.1 &#956;g g&#8722;1 and LOQ: 3.7 &#956;g g&#8722;1 of tissue). The resulted clean up of the sample, the improvement in peak shape and baseline and the reduction of interferences, evidence that the MIPNPs could potentially be applied as sorbent in a DMSPE with satisfactory results and with a minimum amount of sorbent (1 mg).","palabras_clave":"Coenzyme Q10|Molecularly imprinted polymer nanoparticles| Dispersive micro solid phase extraction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5614,"idioma_id":4,"titulo":"Sinorhizobium meliloti low molecular mass phosphotyrosine phosphatase SMc02309 modifies activity of the UDP-glucose pyrophosphorylase ExoN involved in succinoglycan biosynthesis","resumen":"In Gram-negative bacteria, tyrosine phosphorylation has been shown to play a role in the control of exopolysaccharide (EPS) production. This report demonstrates that the chromosomal open reading frame SMc02309 from Sinorhizobium meliloti 2011, encodes a protein with significant sequence similarity to low molecular weight protein tyrosine phosphatases (LMW-PTPs), such as the Escherichia coli Wzb. Unlike other well-characterized EPS biosynthesis gene clusters, which contain neighboring LMW-PTPs and kinase, the S. meliloti succinoglycan (EPS I) gene cluster located on megaplasmid pSymB does not encode a phosphatase. Biochemical assays revealed that the SMc02309 protein hydrolyzes p-nitrophenyl phosphate (p-NPP) with kinetic parameters similar to other bacterial LMW-PTPs. Furthermore, we show evidence that SMc02309 is not the LMW-PTP of the bacterial tyrosine-kinase (BY-kinase) ExoP. Nevertheless ExoN, a UDP-glucose pyrophosphorylase involved in the first stages of EPS I biosynthesis, is phosphorylated at tyrosine residues and constitutes an endogenous substrate of the SMc02309 protein. Additionally, we show that the UDP-glucose pyrophosphorylase activity is modulated by SMc02309-mediated tyrosine dephosphorylation. Moreover, a mutation in the SMc02309 gene decreases EPS I production and delays nodulation on M. sativa roots.","palabras_clave":"PHOSPHOTYROSINE-PROTEIN PHOSPHATASE|EXOPOLYSACCHARIDES|BACTERIAL PROTEIN PHOSPHORYLATION|SYMBIOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5615,"idioma_id":4,"titulo":"First Record of Drosophila buzzatii (Patterson & Wheeler) (Diptera: Drosophilidae) Emerging from a Non-Cactus Host","resumen":"Drosophila buzzatii (Patterson & Wheeler), a typical cactophilic species ofthe repleta group, is registered for the first time emerging from Melon(Cucumis melo) in western Argentina. The analysis of inversion polymorphismandgenetic diversity ofmitochondrial cytochrome oxidase subunit Igene (mtCOI) provided additional evidence that corroborated the presenceof a high proportion of D. buzzatii among the flies emerged frommelon. This finding set the scenario for a broader range of possible hostsand host-related distribution and dispersion for this widespread species.","palabras_clave":"CACTOPHILIC FLIES|INVERSION CHROMOSOME POLYMORPHISM|HOST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5616,"idioma_id":4,"titulo":"Tissue discrimination in magnetic resonance imaging of the rotator cuff","resumen":"Evaluation and diagnosis of diseases of the muscles within the rotator cuff can be done using different modalities, being the Magnetic Resonance the method more widely used. There are criteria to evaluate the degree of fat infiltration and muscle atrophy, but these have low accuracy and show great variability inter and intra observer. In this paper, an analysis of the texture features of the rotator cuff muscles is performed to classify them and other tissues. A general supervised classification approach was used, combining forward-search as feature selection method with kNN as classification rule. Sections of Magnetic Resonance Images of the tissues of interest were selected by specialist doctors and they were considered as Gold Standard. Accuracies obtained were of 93% for T1-weighted images and 92% for T2-weighted images. As an immediate future work, the combination of both sequences of images will be considered, expecting to improve the results, as well as the use of other sequences of Magnetic Resonance Images. This work represents an initial point for the classification and quantification of fat infiltration and muscle atrophy degree. From this initial point, it is expected to make an accurate and objective system which will result in benefits for future research and for patients' health.","palabras_clave":"Magnetic Renosance|Rotator cuff|Texture|Image Segmentation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5617,"idioma_id":4,"titulo":"Large-scale model of the axisymmetric kinematic dynamo","resumen":"A formulation of a kinematic dynamo is presented, based on a previously derived selfconsistentprocedure for obtaining large-scale models for complex system of equations. Themodel has only a small number of parametrized variables: the small-scale magnetic diffusivity,the scale of the large-scale fields, and a factor in the explicit expression of the&#945;&#981;&#981; component of the &#945; tensor. Explicit expressions of what corresponds to the other componentsof the &#945; tensor and of the diffusivity tensor are derived in terms of the large-scalemeridional flow and of the differential rotation law, without introducing additional parameters.A very simple simulation of a solar-like dynamo, employing the model without meridionalflow shows reasonable magnetic field evolution, with a cycle duration of about 2\/3 that of theSun, shift of the magnetic field from mid-latitudes towards the equator, poleward migrationof the radial field at high latitudes, and correct phase relation between radial and azimuthalcomponents.","palabras_clave":"dynamo|magnetic fields|MHD|turbulence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5618,"idioma_id":4,"titulo":"Addition of vasopressin synthetic analogue [V4Q5]dDAVP to standard chemotherapy enhances tumour growth inhibition and impairs metastatic spread in aggressive breast tumour models","resumen":"[V4Q5]dDAVP is a novel 2nd generation vasopressin analogue with robust antitumour activity against metastatic breast cancer. We recently reported that, by acting on vasopressin V2r membrane receptor present in tumour cells and microvascular endothelium, [V4Q5]dDAVP inhibits angiogenesis and metastatic progression of the disease without overt toxicity. Despite chemotherapy remaining as a primary therapeutic option for aggressive breast cancer, its use is limited by low selectivity and associated adverse effects. In this regard, we evaluated potential combinational benefits by adding [V4Q5]dDAVP to standard-of-care chemotherapy. In vitro, combination of [V4Q5]dDAVP with sub-IC50 concentrations of paclitaxel or carmustine resulted in a cooperative inhibition of breast cancer cell growth in comparison to single-agent therapy. In vivo antitumour efficacy of [V4Q5]dDAVP addition to chemotherapy was first evaluated using the triple-negative MDA-MB-231 breast cancer xenograft model. Tumour-bearing mice were treated with i.v. injections of [V4Q5]dDAVP (0.3 &#956;g\/kg, thrice weekly) in combination with weekly cycles of paclitaxel (10 mg\/kg i.p.). After 6 weeks of treatment, combination regimen resulted in greater tumour growth inhibition compared to monotherapy. [V4Q5]dDAVP addition was also associated with reduction of local aggressiveness, and impairment of tumour invasion and infiltration of the skin. Benefits of combined therapy were confirmed in the hormone-independent and metastatic F3II breast cancer model by combining [V4Q5]dDAVP with carmustine (25 mg\/kg i.p.). Interestingly, [V4Q5]dDAVP plus cytotoxic agents severely impaired colony forming ability of tumour cells and inhibited breast cancer metastasis to lung. The present study shows that [V4Q5]dDAVP may complement conventional chemotherapy by modulating metastatic progression and early stages of microtumour establishment, and thus supports further preclinical testing of the compound for the management of aggressive breast cancer.","palabras_clave":"ANTIMETASTATIC|CHEMOTHERAPY|COMBINATIONAL THERAPY|HORMONE-INDEPENDIENT BREAST CANCER|SYNTHETIC PEPTIDE DERIVATIVE|V2R AGONIST|VASOPRESSIN ANALOGUE|[V4Q5]DDAVP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5619,"idioma_id":4,"titulo":"CIGB-300, an anti-CK2 peptide, inhibits angiogenesis, tumor cell invasion and metastasis in lung cancer models","resumen":"Casein kinase 2 (CK2) is overexpressed in several types of cancer. It has more than 300 sub-strates mainly involved in DNA reparation and replication, chromatin remodeling and cellular growth. Inrecent years CK2 became an interesting target for anticancer drug development. CIGB-300 is a peptidicinhibitor of CK2 activity, designed to bind to the phospho-acceptor domain of CK2 substrates, impairingthe correct phosphorylation by the enzyme. The aim of this work was to explore the antitumor effects ofthis inhibitor in preclinical lung cancer models.Materials and methods: Human H125 and murine 3LL Lewis lung carcinoma cell lines were used to evaluatethe effect of CIGB-300 treatment in vitro. For this purpose, adhesion, migration and invasion capabilitiesof cancer cells were tested. Proteolytic activity of tumor cell-secreted uPA and MMP after CIGB-300incubation was also analyzed.In vivo anticancer efficacy of the peptide was evaluated using experimental and spontaneous lungcolonization assays in C57BL\/6 mice. Finally, in order to test the effect of CIGB-300 on tumor cell-inducedangiogenesis, a modified Matrigel plug assay was conducted.Results and conclusion: We demonstrate that treatment with low micromolar concentrations of CIGB-300caused a drastic reduction of adhesion, migration and invasion of lung cancer cells. Reduced invasivenessafter CIGB-300 incubation was associated with decreased proteolytic activity of tumor cell-conditionedmedium.In vivo, intravenous administration of CIGB-300 (10 mg\/kg) markly decreased lung colonization andmetastasis development of 3LL cells. Interestingly, after 5 days of systemic treatment with CIGB-300,tumor cell-driven neovascularization was significantly reduced in comparison to control group. Alto-gether our data suggest an important role of CK2 in lung tumor development, suggesting a potential useof CIGB-300 as a novel therapeutic agent against lung cancer.","palabras_clave":"CIGB-300|CK2|METASTASIS|LUNG CANCER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5620,"idioma_id":4,"titulo":"Vestiges of Ent3p\/Ent5p function in the giardial epsin homolog","resumen":"An accurate way to characterize the functional potential of a protein is to analyze recognized protein domains encoded by the genes in a given group. The epsin N-terminal homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated trafficking. In this work, we investigate the function of the single ENTH-containing protein from the protist Giardia lamblia by testing its function in Saccharomyces cerevisiae. This protein, named GlENTHp (for G. lamblia ENTH protein), is involved in Giardia in endocytosis and in protein trafficking from the ER to the vacuoles, fulfilling the function of the ENTH proteins epsin and epsinR, respectively. There are two orthologs of epsin, Ent1p and Ent2p, and two orthologs of epsinR, Ent3p and Ent5p in S. cerevisiae. Although the expression of GlENTHp neither complemented growth in the ent1&#916;ent2&#916; mutant nor restored the GFP-Cps1 vacuolar trafficking defect in ent3&#916;ent5&#916;, it interfered with the normal function of Ent3\/5 in the wild-type strain. The phenotype observed is linked to a defect in Cps1 localization and &#945;-factor mating pheromone maturation. The finding that GlENTHp acts as dominant negative epsinR in yeast cells reinforces the phylogenetic data showing that GlENTHp belongs to the epsinR subfamily present in eukaryotes prior to their evolution into different taxa.","palabras_clave":"Endosomal Traffic|Giardia Lamblia|saccharomyces cerevisiae|epsin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5621,"idioma_id":4,"titulo":"?Groundwater chemical baseline values to assess the Recovery Plan in the Matanza-Riachuelo River basin, Argentina?","resumen":"The two most exploited aquifers in the Matanza-Riachuelo River basin are being monitored in the framework of the Integrated Environmental Sanitation Plan that implements the Basin Authority, Autoridad de Cuenca Matanza Riachuelo. In this context, this work identifies the groundwater chemical types and the natural processes behind them; determines spatial and temporal changes; establishes ranges of variation for chemical components, and proposes concentration values for the upper limit of the natural chemical background. A total of 1007 samples from three aquifer-layers (Upper Aquifer, top and bottom of Puelche Aquifer) have been studied. As concrete guidelines for practical determination of baseline values are not available in the region, the methodology used follows the proposals of European projects which assessed European water directives. The groundwater composition is very stable in terms of both chemical facies and mineralization degree, and the changes observed in the dry and wet periods analysed are subtle in general. Most of the groundwater is Na-HCO3 type, except a fewsamples that are Ca-HCO3, Na-ClSO4 and Na-Cl types. The Ca-HCO3 waters are the result of calcium carbonate dissolution, Na-HCO3 waters result from cation exchange and carbonate dissolution, while in the Na-ClSO4 and Na-Cl waters, mixing with connate and with encroached old marine water from the underlying and overlying sediments are the most relevant processes. The proposed values for the upper limit of the natural background consider the influence of geology and Holocene marine ingressions in the baseline of coastal groundwater. This study allowed to know the initial chemical conditions of the groundwater system of the Matanza-Riachuelo River basin and to establish the reference from which Basin Authority can start to evaluate trends and monitor the recovery plan. At the same time, it sets a precedent for future studies in the region.","palabras_clave":"Groundwater baseline|Matanza-Riachuelo River basin|Pampean Sediments|Puelche Aquifer"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5622,"idioma_id":4,"titulo":"Fine Sediment Input and Benthic Fauna Interactions at the Confluence of Two Large Rivers","resumen":"Several studies suggest that invertebrate abundance and richness are disrupted and reset at confluences. Thus, junctions contribute disproportionately to the overall aquatic biodiversity of the river. In general terms, authors have reported high abundance and diversity due to the major physical heterogeneity at junctions. However, data are still scarce and uncertainties are plentiful. The impact of a great input of fine sediments on the distribution patterns of benthic invertebrates at a river confluence was quantitatively analyzed herein. The junction of the subtropical Bermejo River (high suspended sediment load) with the large Paraguay River is the selected study area to achieve this aim. While diversity increased slightly downstream the junction (from 0.21 to 0.36), density and richness of the macroinvertebrate assemblage significantly diminished downstream the confluence (from 29050 to 410 ind\/m2; p< 0.05) due to the input of fine sediment from the Bermejo River (mean fine sediment increased downstream from 6.3 to 10.2 mg\/L), causing a negatively impact on invertebrate assemblage. This study highlights the ecological importance of the sediment input effects on benthic invertebrates, a topic still poorly explored in river ecology. It is speculated that the spatial extent of the impact would be dependent upon the hydrological and sedimentological context, highly unequal between both rivers. New hypotheses should be tested through new studies considering different hydrological stages.","palabras_clave":"BENTHIC INVERTEBRATE|FINE SEDIMENT LOAD|CHANNEL CONFLUENCE|LARGE RIVER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5623,"idioma_id":4,"titulo":"A multi-analytical investigation of the materials and painting technique of a wall painting from the church of Copacabana de Andamarca (Bolivia)","resumen":"The Andean church of Our Lady of Copacabana de Andamarca in Bolivia was built in 1723. Its walls are made of adobe bricks and are decoratedwith paintings dated frommid 18th century. Before a restoration process involving the governments of Bolivia and the Federal Republic of Germany, seven microsamples were extracted from representative colors of one of the wall paintings inside the church. The aim of our research was to characterize the chromatic palette and investigate the painting technique of this Andean colonial wall painting. To approach these goals, an integrated investigation comprising Raman and FTIR-ATR spectroscopy, optical microscopy (OM), scanning electron microscopy with X-ray microanalysis (SEM?EDS), gas chromatography (GC), and gas chromatography mass spectrometry (GC?MS) was carried out.The results indicated the use of a mixture of two basic copper sulfates of mineral origin, brochantite and antlerite, as the green pigment, adding relevant information to the palette of green pigments in colonial art. Yellow, orange and red ochre, abundant pigments in the Andean region,were also characterized. Observation by optical microscopy of cross-sections of the embedded microsamples and analysis of organic binders by GC and GC?MS pointed to a secco technique and the use of a mixture of egg and vegetable oil (?tempera grassa?) as the pigment binder.In conclusion, our results allowed to establish the pigment palette and offered new insights into the painting technique of Andean wall paintings.","palabras_clave":"SEM-EDS|RAMAN AND IR SPECTROSCOPY|GAS CHROMATOGRAPHYMASS SPECTROMETRY|WALL PAINTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5624,"idioma_id":4,"titulo":"Noise exposure and oxidative balance in auditory and extra-auditory structures in adult and developing animals. Pharmacological approaches aimed to minimize its effects","resumen":"Noise coming from urban traffic, household appliances or discotheques might be as hazardous to the health of exposed people as occupational noise, because may likewise cause hearing loss, changes in hormonal, cardiovascular and immune systems and behavioral alterations. Besides, noise can affect sleep, work performance and productivity as well as communication skills. Moreover, exposure to noise can trigger an oxidative imbalance between reactive oxygen species (ROS) and the activity of antioxidant enzymes in different structures, which can contribute to tissue damage. In this review we systematized the information from reports concerning noise effects on cell oxidative balance in different tissues, focusing on auditory and non-auditory structures. We paid specific attention to in vivo studies, including results obtained in adult and developing subjects. Finally, we discussed the pharmacological strategies tested by different authors aimed to minimize the damaging effects of noise on living beings.","palabras_clave":"NOISE|AUDITORY SYSTEM|OXIDATIVE BALANCE|ROS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5625,"idioma_id":4,"titulo":"Albumin-folate-conjugates for drug-targeting in photodynamic therapy","resumen":"Photodynamic therapy (PDT) is based on thecytotoxicity of photosensitizers in the presence of light. Increasedselectivity and effectivity of the treatment is expected if a specific uptakeof the photosensitizers into the target cells, often tumor cells, can beachieved. An attractive transporter for that purpose is the folic acid receptor a (FRa), which is over-expressed onthe surface of many tumor cells and mediates an endocytotic uptake. Here, wedescribe the synthesis and photobiological characterization of polar b-carboline derivatives asphotosensitizers covalently linked to folate-tagged albumin as carrier system.The particles were taken up by KB (humannasopharyngeal carcinoma) cells within &lt; 90 min and then co-localized with alysosomal marker. FRa antibodies prevented the uptake and also thecorresponding conjugate without folate was not taken up. Accordingly,a folate-albumin-b-carbolinium conjugate provedto be phototoxic, while the corresponding albumin-b-carbolinium conjugateswithout FA were non-toxic, both with and without irradiation. An excess of freefolate as competitor for the FRa-mediateduptake completely inhibited the photocytotoxicity. Interestingly, the albuminconjugates are devoid of photodynamic activity under cell-free conditions, asshown for DNA as a target. This indicates that the reactive species generatedby photoexcitation of the chromophore are efficiently scavenged by the attachedalbumin so that phototoxiciy requires cellular uptake and lysosomal degradationof the conjugates.","palabras_clave":"photosensitization|photodynamic therapy|b-carbolines|albumin conjugates|drug delivery"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5626,"idioma_id":4,"titulo":"Electrochemical conversion of sulfur dioxide with a rotating cylinder electrode working as anode or cathode","resumen":"BACKGROUND: A batch electrochemical reactor with a  rotating cylinder electrode is analysed for the transformation of sulphur  dioxide into either sulphuric acid or colloidal sulphur.     RESULTS: Potentiostatic experiments  carried out at 30 °C  and 500 rpm with 5 g L-1   SO2   in 0.5 mol L-1   H2SO4    conclude that -0.7 V represents an appropriate potential for the sulphur  production at a 316L stainless steel cathode. The figures of merit were: 0.15  kg m-3   h-1   space time yield and 39.7 kWh kg-1    for specific energy consumption. Galvanostatic experiments at 30 °C and 1000 rpm with  three-dimensional electrodes identify graphite felt as a promising anodic  material. Using a gas phase of 5% SO2   in nitrogen and 0.5 mol L-1    H2SO4   as supporting electrolyte, a macrokinetic current  density of 100 mA cm-1    represents an appropriate value, being the space time yield 7.58 kg m-3    h-1   with 2.86 kWh kg-1   specific energy consumption.     CONCLUSION: An electrochemical  reactor with a rotating cylinder electrode showed a good performance for the  production of colloidal sulphur. Sulphur  dioxide was also converted into sulphuric acid including a separator in a  reactor with a three-dimensional rotating cylinder anode and co-current gas and  liquid flows","palabras_clave":"SULFUR DIOXIDE|ELECTROCHEMICAL REACTOR|ROTATING CYLINDER ELECTRODE|SULFUR PRODUCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5627,"idioma_id":4,"titulo":"Electropolimerization of Functionalizaed Carbazole End-Capped Dendrimers. Formation of Conductive Films","resumen":"A series of peripherally carbazole functionalized dendrimeric starburst monomers were synthesized and characterized. The electrochemical polymerization over Pt and indium tin oxide electrodes of fully p-conjugated dendrimers allows the generation of electro-active polymeric films. Dendrimers were design with and without electroactive central core (triphenylamine) connected to peripherals moieties, which influence the physicochemical characteristics of the electrogenerated polymers. The versatile electropolymerization methodology allows the generation of thin films of hyperbranched macro-molecules with good electrical conductivity, reversible electrochemical processes and electrochromic properties.","palabras_clave":"DENDRIMERIC POLYMERS|ELECTRODEPOSITION|OPTOELECTRONIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5628,"idioma_id":4,"titulo":"Experimental and theoretical insights in alkene-arene intramolecular &#960;-stacking interaction","resumen":"Chiral acrylic esters derived from biomass were developed as models to have a better insight in the aryl-vinyl pi-stacking interactions. Quantum chemical calculations, NMR studies and experimental evidences demonstrate the presence of an equilibrium of at least four different conformations: pi-stacked and face-to-edge, each of them in a s-cis\/s-trans conformation. The results show that the stabilization produced by the pi-pi interaction makes the pi-stacked conformation predominant in solution and this stabilization is slightly affected by the electron density of the aromatic counterpart.","palabras_clave":"PI-STACKING INTERACTION|ASYMMETRIC SYNTHESIS|BIOMASS|ACRYLIC ESTERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5629,"idioma_id":4,"titulo":"Firm-level determinants of SME´s financing decisions. Evidence from Argentina","resumen":"This paper studies the determinants of small and medium enterprises (SMEs) financing decisions, which we characterise through three cases: trade-off behaviour, pecking order and extreme aversion to debt.  We test our hypotheses using a dataset of firms from Bahía Blanca (Argentina) for two years: 2006 and 2010. We find that firm characteristics related to information asymmetries, such as firm age, size and legal form, and personal factors, as owner?s age and education, and perception of emotional bankruptcy costs, are relevant variables in SME? financing behaviour. The recognition of extreme aversion to debt leads to reconsider the underleverage problem of SMEs","palabras_clave":"Aversion to debt; |financing decisions|pecking order;|small firms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5630,"idioma_id":5,"titulo":"Early cardiovascular changes in young people with normal and normal-high blood pressure","resumen":"Introducción: El incremento de la masa ventricular izquierda y la disminución de ladistensibilidad arterial (DA) constituyen factores de riesgo cardiovascularindependientes. Objetivos: analizar en jóvenes con presión arterial óptima(PAO), normal (PAN) y normal alta (PANA) aspectos estructurales y funcionalesdel corazón y la aorta torácica con el fin de evaluar la existencia deanomalías precoces relacionadas con los niveles de presión arterial. Material y métodos: se registró lapresión arterial y se evaluó la masa y función del ventrículo izquierdo (VI) yla DA por ecocardiografía. Resultados:se estudiaron 754 jóvenes (271 varones; edad: 20.47±1.35 años), 54% conPAO, 32% PAN y 14% PANA. EI&nbsp; índice demasa VI fue mayor en PAN y PANA, respecto a PAO (28.49 ± 0.29; 30.90 ± 0.44;31.26 ± 0.73 g\/m2.7respectivamente;- p&lt;0.05). El acortamientomedio ventricular corregido fue similar entre PAO (99.8±0.8%) y PAN (99.2±1.1%-NS), mientras que menor en PANA (95.4±1.9%-p&lt;0.05 vs. PAO). La relacióne´\/a´ indicadora de la función diastólica del VI fue mayor en PAO (2.18±0.03)que en PANA (2.03±0.06-p&lt;0.03); mientras que los sujetos con presiónarterial PAN tuvieron valores intermedios (2.10±0.05-NS). La DA fue menor en PANAque en PAN y PAO (1.41±0.05; 1.22±0.02 y 1.14±0.01 mmHg\/cm3\/m2,respectivamente; p&lt; 0.01). Conclusiones:En jóvenes no hipertensos pero con valores de presión arterial superiores alos óptimos se detectaron alteraciones cardiovasculares estructurales yfuncionales que posiblemente permitan identificar tempranamente a una poblacióncon mayor riesgo cardiovascular.","palabras_clave":"HIPERTROFIA|DISTENSIBILIDAD ARTERIAL|HIPERTENSION|RIESGO CARDIOVASCULAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5631,"idioma_id":4,"titulo":"Long-term study of the life cycle of the freshwater snail Heleobia parchappii (Mollusca: Cochliopidae) in a lentic environment in Argentina","resumen":"The gastropod Heleobia parchappii is distributed in lentic and lotic environments in the Pampas region of Argentina and is ahost for at least 24 species of digeneans. Because ecoparasitological investigations of snail-digenean associations frequentlylack knowledge of the structure and dynamics of the snail host population, the purpose of this study is a long-term analysisof the basic aspects of the H. parchappii life cycle as a preliminary step of an environmental parasitology study. Monthlycollections were made in Nahuel Rucá Lagoon from August 2010 to February 2013, and a total of 24 931 individuals ofH. parchappii were measured and size-frequency distributions were constructed. The von Bertalanffy growth equation andthe von Bertalanffy equation with seasonal oscillation growth equations were fit to shell length data. The size-frequencydistributions of H. parchappii fit a polymodal distribution, and 14 age classes were identified. The breeding periods of H.parchappii in 2011 were in summer (February and March), autumn (June) and spring (October). However, in 2012 the periodsof recruitment were in autumn (May), winter (July) and spring (October). The von Bertalanffy models best fit the data, and thelikelihood ratio test comparison showed significant differences in the estimated parameters between cohorts. This is the firststudy in which three cohorts of H. parchappii were followed from recruitment until death, and a life cycle of approximately20.33 months was estimated. The observed differences in the recruitment periods and growth parameters could be a strategyby the snail to prevent the negative effects on reproductive success caused by digeneans. We suggest that long-term studies thatinclude a larger number of environmental and biological factors are needed to fully understand the dynamics of the recruitmentand growth of juveniles of this species across its distribution range.","palabras_clave":"LIFE CYCLE|SHELL SIZE|VON BERTALANFFY GROWTH MODEL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5632,"idioma_id":4,"titulo":"Fossil forests in the Austral Basin (Argentina) marking a Cenomanian heterogeneous forced regressive surface","resumen":"The mid-Cretaceous greenhouse period in the southern Patagonia Argentina (Austral Basin) is represented by the Mata Amarilla Formation. It is composed of three informal sections which were deposited in littoral and continental environments. The boundary between the lower and middle sections of the Mata Amarilla Formation shows a drastic reduction in accommodation\/sediment supply (A\/S) ratio, interpreted as a forced regressive surface. This surface is characterized by a well-developed palaeosol, associated with the extensive preservation of a podocarp-dominated fossil forest over a vast area (more than 5400 km2). Sedimentological and palaeopedological analyses, in conjunction with forest structure, tree density and growth-ring analyses, indicate that the mid-Cenomanian forced regression can be distinguished as a non-uniform surface developed over a short period of time. This sequence boundary is recognized through a heterogeneous regional surface, delimited in the western part of the study area by an erosional surface generated by a large lateral channel migration recorded by sheet-like channel deposits with transported logs. By comparison, towards the eastern part of the study area, it appears as a paraconformity bounded by a very mature vertic Alfisol, which may have taken 40?100 ky to develop, and the preservation of a fossil forest in life position with a minimum age of 337 years. It is concluded that the extensive presence of these fossil forests at the same stratigraphic level in a vast region respond to changes in A\/S ratio in a forced regressive surface at basin scale.","palabras_clave":"FOSSIL FOREST|CRETACEOUS|MATA AMARILLA FORMATION|AUSTRAL BASIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5633,"idioma_id":4,"titulo":"Moderate exercise prevents functional remodeling of the anterior pituitary gland in diet-induced insulin resistance in rats: role of oxidative stress and autophagy","resumen":"A sustained elevation of glucocorticoid production, associated with the establishment of insulin resistance (IR) could add to the deleterious effects of the IR state. The aim of this study is to analyze the consequences of long-term feeding with a sucrose-rich diet (SRD) on Pomc\/ACTH production, define the underlying cellular processes, and determine the effects of moderate exercise (ME) on these parameters. Animals fed a standard chow with or without 30% sucrose in the drinking water were subjected to ME. Circulating hormone levels were determined, and pituitary tissues were processed and analyzed by immunobloting and quantitative real-time PCR. Parameters of oxidative stress (OxS), endoplasmic reticulum stress, and autophagy were also determined. Rats fed SRD developed a decrease in pituitary Pomc\/ACTH expression levels, increased expression of antioxidant enzymes, and induction of endoplasmic reticulum stress and autophagy. ME prevented pituitary dysfunction as well as induction of antioxidant enzymes and autophagy. Reporter assays were performed in AtT-20 corticotroph cells incubated in the presence of palmitic acid. Pomc transcription was inhibited by palmitic acid-dependent induction of OxS and autophagy, as judged by the effect of activators and inhibitors of both processes. Long-term feeding with SRD triggers the generation of OxS and autophagy in the pituitary gland, which could lead to a decline in Pomc\/ACTH\/glucocorticoid production. These effects could be attributed to an increase in fatty acids availability to the pituitary gland. ME was able to prevent these alterations, suggesting additional beneficial effects of ME as a therapeutic strategy in the management of IR.","palabras_clave":"PITUITARY|STRESS|EXERCISE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5634,"idioma_id":4,"titulo":"Role of CREB on Heme Oxygenase-1 Induction in Adrenal Cells. Involvement of the PI3K Pathway","resumen":"In addition to the well-known function of ACTH as the main regulator of adrenal steroidogenesis, we have previously demonstrated its effect on the transcriptional stimulation of HO-1 expression, a component of the cellular antioxidant defense system. In agreement, we hereby demonstrate that, in adrenocortical Y1 cells, HO-1 induction correlates with a significant prevention of the generation of reactive oxygen species induced by H2O2\/Fe(2+) ACTH\/cAMP-dependent activation of redox-imbalanced related factors such as NRF2 or NF&#954;B and the participation of MAPKs in this mechanism was, however, discarded based on results with specific inhibitors and reporter plasmids. We suggest the involvement of CREB in HO-1 induction by ACTH\/cAMP, as transfection of cells with a dominant-negative isoform of CREB (DN-CREB-M1) decreased, while overexpression of CREB increased HO-1 protein levels. Sequence screening of the murine HO-1 promoter revealed CRE-like sites located at -146 and -37 of the transcription start site and ChIP studies indicated that this region recruits phosphorylated CREB (pCREB) upon cAMP stimulation in Y1 cells. In agreement, H89 (PKA inhibitor) or cotransfection with DN-CREB-M1 prevented the 8Br-cAMP-dependent increase in luciferase activity in cells transfected with pHO-1[-295\/+74].LUC. ACTH and cAMP treatment induced the activation of the PI3K\/Akt signaling pathway in a PKA-independent mechanism. Inhibition of this pathway prevented the cAMP-dependent increase in HO-1 protein levels and luciferase activity in cells transfected with pHO-1[-295\/+74].LUC. Finally, here we show a crosstalk between the cAMP\/PKA and PI3K pathways that affects the binding of p-CREB to its cognate element in the murine promoter of the Hmox1 gene.","palabras_clave":"CREB|Hemoxiganasa 1|AKT|steroidogenesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5636,"idioma_id":4,"titulo":"Serologically silent, occult equine infectious anemia virus (EIAV) infections in horses","resumen":"Molecular and serological techniques for Equine Infectious Anemia Virus (EIAV) diagnosis were compared using samples from 59 clinically normal horses stabled on five farms in the Santa Fe Province of Argentina. Of these 26 (44.1%) were positive in official AGID tests and\/or gp45\/gp90-based ELISA. Surprisingly 18 of the 33 seronegative horses were positive in a PCR against viral sequences encoding gp45 (PCR-positive\/AGID-negative) with all but one remaining EIAV-antibody negative throughout a two year observation period. The gp45 PCR results are supported by fact that 7\/18 of these horses were positive in the Office International des Epizooties (OIE) recommended EIAV gag gene specific PCR plus 2 of this 7 also reacted in a PCR directed predominantly against the 50 untranslated region of the viral genome. Furthermore sufficient quantities of serum were available from 8 of these horses to verify their seronegative status in sensitive Western Blot tests and demonstrate by ELISA the absence of EIAV-specific antibodies was not attributable to abnormalities in total IgG concentration. Studies involving 7 of the PCR-positive\/AGID-negative horses to measure lymphocyte proliferation in the presence of PHA showed no significant differences between this group and control animals. In addition, lymphocytes from 2 of these 7 horses responded to peptides derived from gp90 and gp45. Together these results demonstrate that apparently clinically normal horses with no gross signs of immunodeficiency in terms of total IgG  Concentration or T helper-cell function can remain seronegative for at least 24 months while harboring EIAV specific nucleic acid sequences.","palabras_clave":"Equine infectious anemia virus |Horse |AGID |PCR |Western blot |Absence of seroconversion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5637,"idioma_id":4,"titulo":"Comparison of the performance of ultrafiltration and nanofiltration membranes for recovery and recycle of tannins in the leather industry","resumen":"The tanning industries are characterized by generating large volumes of wastewater. The largest volume of effluents comes from the tanning stage and has a high concentration of tannins and a low ratio tannin\/non-tannin (T\/NT). Membrane processes are appropriate technologies for treating these effluents. The aim of this study was to evaluate the performance of polymeric membranes in the treatment of exhausted vegetable tannin liquor from a tanning industry for its reuse. Different ultrafiltration (UF) and nanofiltration (NF) membranes were tested. Permeate flux decline, fouling resistances, fouling index, rejection for tannin (T) and non-tannin (NT) and increasing of the T\/NT ratio in the retentate stream were evaluated. NF membranes had better characteristics than those of UF. The MPF-34 membrane showed the best separation properties with an increasing of the T\/NT ratio from 1.68 to 1.80, for a volume reduction factor of 1.04. DK presented the best permeation properties and the lowest fouling, with an average permeate flux of 17.17 L\/(m2 h) and a fouling index (If) of 27.3%. The results were promising, so this clean technology could be implemented to minimize the negative environmental impact caused by tanning industry.","palabras_clave":"Leather industry|Wastewater treatment|Clean technology|Membrane"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5638,"idioma_id":4,"titulo":"Proteolysis, Texture and Microstructure of Goat Cheese","resumen":"Changes in the structure of cheese are mostly due to changes in the protein matrix, mainly because of the degradation of &#945;- and &#946;- and k-casein. Therefore, the objective of this work was to study the effect of proteolysis on the microstructure and texture of goat cheese during ripening. The cheeses were made using Creole goat milk from the Quebrada de Humahuaca in Jujuy and ripened at 10 °C and 90% RH. Samples were taken after 5 hours of preparation and after 10, 20, 30, 40, 60 and 80 days of ripening. Proteolysis was studied by the evolution of the major fractions of casein (&#945;, &#946; and para-&#954;) determined by HPLC and soluble nitrogen, allowing the calculation of the rate of maturation. The texture profile was determined using a texture analyzer QTS 25. The changes in the protein matrix of the cheese were observed by scanning electron microscopy using a JEOL JSM-6480 LV. We found that the &#945;-casein was hydrolyzed at a low speed at the beginning and until 30 days, between 30 and 40 days of ripening, &#945;-casein was hydrolyzed faster. After this time, this fraction content became stable until the end of the ripening. The rate of hydrolysis of para-&#954;-casein increased starting from 30 days up to 60 days of ripening, when it became stable. It was observed that the initial matrix of cheese protein was formed by free large cavities with a heterogeneous dispersion of casein particles. During ripening, the size of the cavities decreased and the cheese protein matrix became more compact. The size of the holes was reduced and the globular characteristic of the micelles was lost after 40 days of maturation, coinciding with accentuated hydrolysis &#945;-caseins. The soluble nitrogen at pH 4.6, increased significantly until 30 days. After that, it remained statistically unchanged for 80 days. The velocity of maturation determined as soluble nitrogen in TCA, rose steadily until 60 days of ripening. Hardness, gumminess, adhesiveness and chewiness increased sharply at 40 days of maturation. After this time, these parameters increased slowly until the end of the sampling period, when the changes in the microstructure of the cheeses revealed the highest compaction of the matrix. This may be related to the formation of soluble nitrogen and degradation of &#945;-caseins during ripening.","palabras_clave":"Casein|ripening,|structure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5639,"idioma_id":4,"titulo":"Antigenic variation in the intestinal parasite Giardia lamblia","resumen":"Giardia lamblia trophozoites undergo antigenic variation, where one member of the Variant-specific Surface Protein (VSP) family is expressed on the surface of proliferating trophozoites and periodically replaced by another one. Two main questions have challenged researchers since antigenic switching was discovered in Giardia: What are the mechanisms involved? How are they influenced by other cellular processes or by the environment? Two molecular mechanisms have been proposed, both involving small non-coding RNAs. Here we postulate that (a) chromatin remodeling, triggered by environmental factors, also plays an important role in selecting the VSP that will be expressed and (b) the particular VSP structure may not only protect the parasite in the small intestine but also signal the need to exchange the existing VSP for another.","palabras_clave":"PARASITE|ANTIGENIC VARIATION|PROTEIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5640,"idioma_id":4,"titulo":"A Straightforward Synthesis of 5-Methylaaptamine from Eugenol, Employing a 6&#960;-Electrocyclization Reaction of a 1-Azatriene","resumen":"Aaptamine, isolated from tropical marine sponges of the Demospongiae class, is the most prominent member of a growing family of natural products. Many aaptaminoids have been shown to have interesting biological activity. The efficient access to 5-methylaaptamine, an unnatural analogue of aaptamine, was achieved by using economic and naturally-occurring eugenol as the starting material. The synthesis involved the preparation of a 5-aminoeugenol derivative through successive nitration, O-methylation, and nitro group reduction reactions. An Elderfield?Johnson sequence was employed to synthesize the N-tosyl-5-allyl-7,8-dimethoxydihydro-1H-quinolin-4-one ring system. A catalytic double-bond isomerization followed by a carbonyl methoximation and 6-&#960; electrocyclization of the 1-azatriene motif afforded the 2,3-dihydro-1H-benzo[de][1,6]naphthyridine tricyclic intermediate, which underwent a reductive desulfonylation and catalytic dehydrogenation to afford the target product.","palabras_clave":"NATURAL PRODUCTS|TOTAL SYNTHESIS|CYCLIZATION|NITROGEN HETEROCYCLES|ISOMERIZATION|DEHYDROGENATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5641,"idioma_id":4,"titulo":"Dynamics of gelation, textural and microstructural properties of gelatin gels in the presence of casein glycomacropeptide","resumen":"The aim of this work was to study the interaction between gelatin and casein glycomacropeptide (CMP) in the dynamic of gelation and the textural and microstructural properties of the mixed gels. Size particle, dynamic of gelation and textural and microstructural properties of CMP, gelatin and CMP-gelatin systems at pH 3.5 and pH 6.5 were determined. Size particle of gelatin increased by decreasing temperature from 35 °C to 5 °C, while no differences were observed in the size particle of CMP. At pH 6.5 the critical gelling concentration of gelatin was 1.5% and CMP did not gel, but the behavior of mixed systems was similar to gelatin. The more relevant result was observed at pH 3.5 since at concentrations in which CMP and gelatin did not gel on its own, the mixed systems gelled suggesting a synergistic effect.","palabras_clave":"GELATIN|CASEIN GLYCOMACROPEPTIDE|INTERACTION|TEXTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5642,"idioma_id":4,"titulo":"First report of Toxocara cati in the domestic land snail Rumina decollata","resumen":"Toxocarosis is a highly prevalent parasitic infection that affects cats and dogs worldwide. As a zoonotic agent, the presence of Toxocara spp. eggs in the environment is a risk for definite, paratenic and human hosts. The land snail Rumina decollata might be a potential parasite reservoir because of its coprophagy behavior. The prospective role of R. decollata as a potential paratenic host of Toxocara cati for domestic cats was studied. R. decollata specimens and samples of cats? faeces were collected from the open spaces of a public institution of Buenos Aires city, inhabited by a stray cat population. Cats? faeces were processed and snails were digested by artificial digestion method. Morphological and molecular analyses were performed to identify the larval stage. T. cati larval eggs were recovered from 23, 5 % (4\/17) of the sampled faeces. Twenty percent of snail pools (5\/25) were confirmed to be positive for Toxocara spp. third larval stage (L3) by PCR. The mean value of total larvae recovered per gram of snail in all positive pools was 5,1 (IC 95%: - 2,5 a 12,7), total larvae per pool was 8,4 (IC 95%: -8,8 a 25,6), with a maximum 33 L3\/pool. This is the first report of T. cati infective larvae in R. decollata domestic snail as a paratenic host, since the relationship between infection in snails and in cats? faeces could be demonstrated in a common environment.","palabras_clave":"Toxocara cati|Rumina decollata|Paratenic host"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5643,"idioma_id":4,"titulo":"Cranial Morphology of the Late Oligocene Patagonian Notohippid Rhynchippus equinus Ameghino, 1897 (Mammalia, Notoungulata) with Emphases in Basicranial and Auditory Region","resumen":"Notohippidae is a probably paraphyletic family of medium sized notoungulates with complete dentition and early tendency to hypsodonty. They have been recorded from early Eocene to early Miocene, being particularly diverse by the late Oligocene. Although Rhynchippus equinus Ameghino is one of the most frequent notohippids in the fossil record, there are scarce data about cranial osteology other than the classical descriptions which date back to the early last century. In this context, we describe the exceptionally preserved specimen MPEF PV 695 (based on CT scanning technique and 3D reconstruction) with the aim of improving our knowledge of the species, especially regarding auditory region (petrosal, tympanic and surrounding elements), sphenoidal and occipital complexes. Besides a modular description of the whole skull, osteological correlates identified on the basicranium are used to infer some soft-tissue elements, especially those associated with vessels that supply the head, mainly intracranially. One of the most informative elements was the petrosal bone, whose general morphology matches that expected for a toxodont. The endocranial surface, together with the surrounding parietal, basisphenoid, occipital, and squamosal, enabled us to propose the location and communication of main venous sinuses of the lateral head wall (temporal, inferior and sigmoid sinuses), whereas the tympanic aspect and the identification of a posterior carotid artery canal provided strong evidence in support of an intratympanic course of the internal carotid artery, a controversial issue among notoungulates. Regarding the arrangement of tympanic and paratympanic spaces, the preservation of the specimen allowed us to appreciate the three connected spaces that constitute a heavily pneumatized middle ear; the epitympanic sinus, the tympanic cavity itself, and the ventral expansion of the tympanic cavity through the notably inflated bullae. We hope this study stimulates further inquires and provides potentially informative data for future research involving other representatives of the order.","palabras_clave":"NOTOUNGULATA|NOTOHIPPIDAE|RHYNCHIPPUS EQUINUS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5644,"idioma_id":4,"titulo":"Composites of Polyindole nanowires within Silicate and Aluminosilicate hosts with distinct conductive properties","resumen":"Nanostructured silicate SBA-15 and aluminosilicate AlSBA-15 were synthesized inorder to prepare polyindole composites. The Silica mesoporous materials were prepared by solgelmethod and alumination by post-synthesis technique and analyzed by different methods(XRD, BET, TEM, and FTIR). Polyindole\/host composites were prepared by an in situ oxidativepolymerization of pre-adsorbed indole, employing Cl3Fe as an oxidant. TG, FTIR, BET, XRD,SEM and TEM were used to characterize the resulting composites. These studies show that afterpolymerization, the porous structures of the materials are preserved and the Polyindole is foundwithin the porous channels. The composites have an electrical conductivity range between valueshigher than those of the pure chemically synthesized Polyindole, close to those of the electricalconductivity of pure electrochemically synthesized polymer and lower than those of the purechemically synthesized polymer, in the order of 10-8 S\/cm.","palabras_clave":"Composites|Nanowire|Polyindol|Host"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5645,"idioma_id":4,"titulo":"Dynamics of Exposure to Rickettsia parkeri in Cattle in the Paran\u0002a River Delta, Argentina","resumen":"Several cases of human rickettsiosis caused by Rickettsia parkeri were recently documented in the Paraná River delta of Argentina, where the tick vector is Amblyomma triste Koch. As cattle suffer recurrent A. triste infestations, they are at risk of becoming infected with R. parkeri. Herein we investigated the dynamics of R. parkeri and its A. triste vector in a herd of beef cattle. Cattle were followed for 18mo and samples were analyzed for the presence of antibodies against four Rickettsia species (R. parkeri, Rickettsia bellii, Rickettsia amblyommii, and Rickettsia felis) and also for the presence of rickettsial DNA. Additionally, cattle were examined for attached ticks and questing adult ticks were collected. All ticks were analyzed for the presence of rickettsial DNA. No evidence of rickettsemia was found in any cow, but the high R. parkeri infection rate documented in A. triste both questing in the study area (13.9%) and feeding on cattle (19.8%) and the identification of antibodies against R. parkeri antigen in 90% of cattle are evidence that infection is taking place. Altogether, our data suggest that A. triste ticks are capable of naturally exposing cattle to R. parkeri. However, the progress of R. parkeri infection and its impact on bovine health and production remain to be established.","palabras_clave":"RICKETTSIA PARKERI|AMBLYOMMA TRISTE|CATTLE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5646,"idioma_id":4,"titulo":"Tolerance of DNA mismatches in Dmc1 recombinase-mediated DNA strand exchange","resumen":"Recombination between homologous chromosomes is required for the faithful meiotic segregation of chromosomes and leads to the generation of genetic diversity. The conserved meiosis-specific Dmc1 recombinase catalyzes homologous recombination triggered by DNA double strand breaks through the exchange of parental DNA sequences. Although providing an efficient rate of DNA strand exchange between polymorphic alleles, Dmc1 must also guard against recombination between divergent sequences. How DNA mismatches affect Dmc1-mediated DNA strand exchange is not understood. We have used fluorescence resonance energy transfer to study the mechanism of Dmc1-mediated strand exchange between DNA oligonucleotides with different degrees of heterology. The efficiency of strand exchange is highly sensitive to the location, type, and distribution of mismatches. Mismatches near the 3' end of the initiating DNA strand have a small effect, whereas most mismatches near the 5' end impede strand exchange dramatically. The Hop2-Mnd1 protein complex stimulates Dmc1-catalyzed strand exchange on homologous DNA or containing a single mismatch. We observed that Dmc1 can reject divergent DNA sequences while bypassing a few mismatches in the DNA sequence. Our findings have important implications in understanding meiotic recombination. First, Dmc1 acts as an initial barrier for heterologous recombination, with the mismatch repair system providing a second level of proofreading, to ensure that ectopic sequences are not recombined. Second, Dmc1 stepping over infrequent mismatches is likely critical for allowing recombination between the polymorphic sequences of homologous chromosomes, thus contributing to gene conversion and genetic diversity.","palabras_clave":"DNA Mismatch|Dmc1|Recombination|meiosis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5647,"idioma_id":4,"titulo":"The nematode Caenorhabditis elegans as an integrated toxicological tool to assess water quality and pollution","resumen":"Determination of water quality status inrivers is critical to establish a sustainable water management policy. For thisreason, over the last decades it has been recommended to perform integrated waterassessments that include water quantities and physicochemical, ecological andtoxicological tests. However, sometimes resources are limited and it is notpossible to perform large-scale chemical determinations of pollutants orconduct numerous ecotoxicological tests. To overcome this problem we use and measurethe growth, as a response parameter, of the soil nematode Caenorhabditis elegans to assess water quality in rivers. The C. elegans is a ubiquitous organism that has emerged as animportant model organism in aquatic and soil toxicology research. The Tunuyán River Basin(Province of Mendoza, Argentina) has been selected as arepresentative traditional water monitoring system to test the applicability ofthe C. elegans toxicological bioassay to generate anintegrated water quality evaluation. Jointly with the C. elegans toxic assays, physicochemicaland bacteriological parameters were determined for each monitoring site. C. elegans bioassays help to identifydifferent water qualities in the river basin. Multivariate statistical analysis(PCA and linear regression models) has allowed us to confirm that traditional water quality studies do notpredict potential toxic effects on living organisms. On the contrary,physicochemical and bacteriological analyzes explain &lt;62% of the C. elegans growthresponse variability, showing that ecotoxicological bioassays are important toobtain a realistic scenario of water quality threats. Our results confirm that the C. elegans bioassay is a sensible andsuitable tool to assess toxicity and should be implemented in routine waterquality monitoring","palabras_clave":"la publicación no tiene|-"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5648,"idioma_id":4,"titulo":"Interaction of atomic hydrogen with anthracene and polyacene from density functional theory","resumen":"tThe interaction of atomic hydrogen with two linear polycyclic aromatic hydrocarbons (PAHs), anthraceneand polyacene (the polymer of benzene), was studied within the density functional theory (DFT). Usinga proper dispersion-corrected method (DFT-D) the preferential physisorption sites were explored. Theactivation barrier for the bond formation between a peripheral C and the incoming H was calculated tobe 58.5 and 34.1 meV with pure DFT on anthracene and polyacene at its antiferromagnetic ground state,respectively. DFT-D, although improves the description of the physisorbed state, tends to underestimatethe chemisorption barriers due an artifact arising from the dispersion correction.","palabras_clave":"---"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5649,"idioma_id":5,"titulo":"Espacios para la memoria: Narrativas sobre los sucesos violentos de la división de Alemania, el bombardeo sobre Japón y la última dictadura militar en Argentina","resumen":"En este trabajo centramos nuestro interés en diversos ?espacios para la memoria? surgidos como recordatorios de un pasado violento. Entre ellos consideramos los sitios donde se da cuenta de la antigua división de Alemania (representados por el tratamiento brindado a los restos del Muro de Berlín); los espacios para la memoria del bombardeo atómico sobre Japón (incluyendo los museos que refieren al tema en Hiroshima y Estados Unidos); los sitios para la reflexión sobre la última dictadura militar en Argentina (contando los ex centros clandestinos de detención, parques, plazoletas, monolitos, etc. en Buenos Aires). Nuestro objetivo reside en discutir la diversidad contenida en los proyectos ensayados. Para ello proponemos abordar la materialidad de los espacios construidos, las ideas y sensaciones a los que apelan, y las narrativas que pretenden construir. El enfoque permite comprender las tensiones que comúnmente involucra la construcción de la memoria en el presente.","palabras_clave":"espacios para la memoria|narrativa|sucesos violentos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5650,"idioma_id":4,"titulo":"Strengthening mechanisms in an Al-Fe-Cr-Ti nano-quasicrystalline alloy and composites","resumen":"We report a study of the structure-processing-property relationships in a high strength Al93Fe3Cr2Ti2 nano-quasicrystalline alloy and composites containing 10 and 20 vol.% ductilising pure Al fibres. The superimposed contributions of several different strengthening mechanisms have been modelled analytically using data obtained from systematic characterisation of the monolithic alloy bar. An observed yield strength of 544 MPa has been substantiated from a combination of solid solution strengthening, work hardening, precipitation hardening and Hall-Petch grain size dependent effects. These materials have been shown by other authors in previous published work to be highly sensitive to the size distribution of particles in the powder from which they are made, and the subsequent thermomechanical processing conditions. The processing condition employed in this study provided micron-sized grains with a strong [111] preferential orientation along the extrusion direction and a bimodal size distribution of the icosahedral nano-quasicrystalline precipitates. Both were deemed to be a significant contributor to the high yield strength observed. The addition of pure Al fibres was found to decrease the yield strength linearly with increasing Al content, and to augment the ductility of the composites.","palabras_clave":"quasicrystals|mechanical properties|strengthening mechanisnms|fibre composites"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5651,"idioma_id":4,"titulo":"Spatial spread of dengue in a non-endemic tropical city in northern Argentina","resumen":"After more than eighty years dengue reemerged in Argentina in 1997. Since then, the largest epidemic in terms of geographical extent, magnitude and mortality, was recorded in 2009. In this report we analyzed the DEN-1 epidemic spread in Orán, a mid-size city in a non-endemic tropical area in Northern Argentina, and its correlation with demographic and socioeconomic factors. Cases were diagnosed by ELISA between January and June 2009. We applied a space-time and spatial scan statistic under a Poisson model. Possible association between dengue incidence and socio-economic variables was studied with the Spearman correlation test. The epidemic started from an imported case from Bolivia and space-time analysis detected two clusters: one on February and other in April (in the south and the northeast of the city respectively) with risk ratios of 25.24 and 4.07 (p &lt;0.01). Subsequent cases spread widely around the city without significant space-temporal clustering. Maximum values of the entomological indices were observed in January, at the beginning of the epidemic (B = 21.96; LH = 8.39). No statistically significant association between socioeconomic variables and dengue incidence was found but positive correlation between population size and the number of cases (p &lt; 0.05) was detected. Two mechanisms may explain the observed pattern of epidemic spread in this non-endemic tropical city: a) Short range dispersal of mosquitoes and people generates clusters of cases and b) long-distance (within the city) human movement contributes to a quasi-random distribution of cases.","palabras_clave":"Dengue|Aedes aegypti|Spatial analysis|Larval indexes|Non-endemic city"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5652,"idioma_id":4,"titulo":"The palynology of the La Deheza Formation (Carboniferous?Permian; Upper Palaeozoic), Paganzo Basin, San Juan Province, Argentina","resumen":"We present the first palynological study of the La Deheza Formation in Paganzo Basin, San Juan Province, Argentina. A total of 18 samples were studied and 116 palynomorph species were recorded. A multivariate statistical (R) analysis confirmed the occurrence of three associations. Assemblages 1 (240 m), 2 (230 m) and 3 (220 m) were referred to biozones already registered in the Paganzo Basin, i.e. Raistrickia densaConvolutispora muriornata (DM), Pakhapites fususVittatina subsaccata (FS) and LueckisporitesWeylandites (LW) biozones. These assemblages can be assigned a PennsylvanianCisularian age based on previous stratigraphic records of the identified species","palabras_clave":"palynology;|La Deheza Formation|Upper Palaeozoic|San Juan|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5653,"idioma_id":4,"titulo":"Long-Lived Binding of Sox2 to DNA Predicts Cell Fate in the Four-Cell Mouse Embryo","resumen":"Transcription factor (TF) binding to DNA is fundamental for gene regulation.However, it remains unknown how the dynamics of TF?DNA interactions change during cell fate determination in vivo. Here, we use photo-activatable FCS to quantify TF?DNA binding in single cells of developing mouse embryos.In blastocysts, the TFs Oct4 and Sox2, which control pluripotency, bind DNA more stably in pluripotent than extraembryonic cells. By contrast, in the 4-cell embryo, Sox2 engages in more long-lived interactions than Oct4. Sox2 long-lived binding varies between blastomeres and is regulated by H3R26 methylation. Live-cell tracking demonstrates that those blastomeres with more long-lived binding contribute more pluripotent progeny, and reducing H3R26 methylation decreases long-lived binding, Sox2 target expression andpluripotent cell numbers. Therefore, Sox2?DNA binding predicts mammalian cell fate as early as the 4-cell stage. More generally, we reveal the dynamic repartitioning of TFs between DNA sites driven by physiological epigenetic changes.","palabras_clave":"TRANSCRIPTION FACTORS|DNA BINDING|EARLY EMBRYO|SOX-2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5654,"idioma_id":4,"titulo":"Draft Genome Sequence of Lactobacillus plantarum CRL1506, an Immunomodulatory Strain Isolated from Goat Milk","resumen":"This report describes a draft genome sequence of Lactobacillus plantarum CRL1506, that was sequenced using a WGS strategy on an Illumina MiSeq sequencer. In total, 5,675,109 paired-end sequenced reads with lengths of 150 bp were obtained, which yielded 1.04 Gb of total sequenced bases with 527-fold coverage. Quality filtered reads were assembled using the Ngen (DNASTAR) assembler giving 14 contigs. The longest contig was 1,048,781 bp and the shortest 22.273 bp. The functional annotation of predicted genes was achieved using the RAST server and the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). tRNAs and rRNAs were identified by ARAGORN and RNAmmer, respectively.","palabras_clave":"Draft Genome Sequence |Lactobacillus plantarum CRL1506|immunomodulatory strain|antiviral activity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5655,"idioma_id":4,"titulo":"Isolation of lactic acid bacteria from swine milk and characterization of potential probiotic strains with antagonistic effects against swine-associated gastrointestinal pathogens","resumen":"Probiotics are usually isolated from the gastrointestinal tract of humans and animals. The search of probiotics in human milk is a recent field of research, as the existence of the human milk microbiome was discovered only about a decade ago. To our knowledge, no reports regarding the potential probiotic effect of bacteria from swine milk were published. In this work, we isolated several lactic acid bacteria from swine milk and evaluated them for them potential as probiotics. Among the isolated strains, Lactobacillus curvatus TUCO-5E showed antagonistic effects against swine-associated gastrointestinal pathogens. TUCO-5E was able to reduce the growth of enterotoxigenic and enterohemorragic E. coli strains as well as pathogenic Salmonella. In vitro exclusion and displacement assays in intestinal epithelial cells showed a remarkable antagonistic effect for L. curvatus TUCO-5E against Salmonella TUCO-I7 and S. enterica ATCC 13096. Moreover, by using a mice model of Salmonella infection we were able to demonstrated that L. curvatus TUCO-5E preventive administration during 5 consecutive days was capable of decreasing the number of S. typhimurium in the liver and spleen of treated mice when compared to controls, and avoided dissemination of the pathogen to the blood stream. Then, we demonstrated here that swine milk is an interesting source for finding beneficial bacteria. In addition, the results of this work suggest that L. curvatus TUCO-5E is a good candidate for in vivo studying the protective effect of probiotics against intestinal infection and damage induced by Salmonella infection in the porcine host.","palabras_clave":"probiotics|swine milk| Salmonella|Lactobacillus curvatus TUCO-5E|intestinal infection"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5656,"idioma_id":4,"titulo":"Immunobiotic Bifidobacteria Strains Modulate Rotavirus Immune Response in Porcine Intestinal Epitheliocytes via Pattern Recognition Receptor Signaling","resumen":"In this work, we aimed to characterize the antiviral response of an originally established porcine intestinal epithelial cell line (PIE cells) by evaluating the molecular innate immune response to rotavirus (RVs). In addition, we aimed to select immunomodulatory bacteria with antiviral capabilities. PIE cells were inoculated with RVs isolated from different host species and the infective titers and the molecular innate immune response were evaluated. In addition, the protection against RVs infection and the modulation of immune response by different lactic acid bacteria (LAB) strains was studied. The RVs strains OSU (porcine) and UK (bovine) effectively infected PIE cells. Our results also showed that RVs infection in PIE cells triggered TLR3-, RIG-I- and MDA-5-mediated immune responses with activation of IRF3 and NF-&#954;B, induction of IFN-&#946; and up-regulation of the interferon stimulated genes MxA and RNase L. Among the LAB strains tested, Bifidobacterium infantis MCC12 and B. breve MCC1274 significantly reduced RVs titers in infected PIE cells. The beneficial effects of both bifidobacteria were associated with reduction of A20 expression, and improvements of IRF-3 activation, IFN-&#946; production, and MxA and RNase L expressions. These results indicate the value of PIE cells for studying RVs molecular innate immune response in pigs and for the selection of beneficial bacteria with antiviral capabilities.","palabras_clave":"immunobiotics|bifidobacteria|rotavirus|PIE cells|TLR3"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5657,"idioma_id":4,"titulo":"Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells","resumen":"Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer?s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. Objective: In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Results: studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1&#61538;, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Conclusions: Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.","palabras_clave":"Lactobacillus jensenii TL2937|antigen presenting cells|phagocytosis|immunobiotics|porcine immunity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5658,"idioma_id":4,"titulo":"Immunobiotic Lactobacillus strains reduce small intestinal injury induced by intraepithelial lymphocytes after Toll-like receptor 3 activation","resumen":"Objective: Intestinal intraepithelial lymphocytes (IELs) play critical roles in disrupting epithelial homeostasis after Toll-like receptor (TLR)-3 activation with genomic rotavirus dsRNA or the synthetic dsRNA analog poly(I:C). The capacity of immunobiotic Lactobacillus rhamnosus CRL1505 (Lr1505) or Lactobacillus plantarum CRL1506 (Lp1506) to beneficially modulate IELs response after TLR3 activation was investigated in vivo using a mice model. Results: Intraperitoneal administration of poly(I:C) induced inflammatory-mediated intestinal tissue damage through the increase of inflammatory cells (CD3+NK1.1+, CD3+CD8&#945;&#945;+, CD8&#945;&#945;+NKG2D+) and pro-inflammatory mediators (TNF-&#945;, IL-1&#946;, IFN-&#947;, IL-15, RAE1, IL-8). Increased expression of intestinal TLR3, MDA5, and RIG-I was also observed after poly(I:C) challenge. Treatment with Lr1505 or Lp1506 prior to TLR3 activation significantly reduced the levels of TNF-&#945;, IL-15, RAE1, and increased serum and intestinal IL-10. Moreover, CD3+NK1.1+, CD3+CD8&#945;&#945;+, and CD8&#945;&#945;+NKG2D+ cells were lower in lactobacilli-treated mice when compared to controls. The immunomodulatory capacities of lactobacilli allowed a significant reduction of intestinal tissue damage. Conclusions: This work demonstrates the reduction of TLR3-mediated intestinal tissue injury by immunobiotic lactobacilli through the modulation of intraepithelial lymphocytes response. It is a step forward in the understanding of the cellular mechanisms involved in the antiviral capabilities of immunobiotic strains.","palabras_clave":"LACTOBACILLI|TLR3|INTESTINAL DAMAGE|INTRAEPITHELIAL LYMPHOCYTES|POLY(I:C)|IMMMUNOBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5660,"idioma_id":4,"titulo":"Temperature-driven self-assembly of self-limiting uniform supraparticles from non-uniform unimolecular micelles","resumen":"In this work, the self-assembly of non-uniform unimolecular micelles constituted of a hyperbranched polyester core decorated with a corona of thermoresponsive poly(N-isopropylacrylamide) (PNIPAm) chains has been studied. As revealed by dynamic light scattering (DLS), transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS), these unimicelles form uniform supraparticles through a thermally-induced self-limited process, as well as exhibit molecular features commonly observed in PNIPAm-based gels. We believe that these results provide new insights into the application of stimuliresponsive polymeric materials as versatile building blocks to build up soft supraparticles displaying well-defined dimensional characteristics.","palabras_clave":"SELF-ASSEMBLY|SUPRALATICES|HYPERBRANCHED POLYMERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5662,"idioma_id":4,"titulo":"Ionic Conductance of Polyelectrolyte-Modified Nanochannels: Nanoconfinement Effects on the Coupled Protonation Equilibria of Polyprotic Brushes","resumen":"A theoretical methodology is introduced to calculate the low-bias conductance, structure, and composition of long polyelectrolyte-modified nanochannels of arbitrary geometry. This methodology is applied to explore the coupling between acid?base equilibrium and geometry in cylindrical, conical, and trumpet-shaped nanochannels modified by end-grafted layers of poly(2-(methacryloyloxy)ethyl-phosphate) (PMEP), a diprotic polyacid. The ionic conductance and speciation curves (i.e., the fraction of deprotonated, monoprotonated, and diprotonated acid segments) for this system were predicted as a function of the solution pH. The apparent pKa?s and widths of the transitions between the different acid?base states determined from the speciation curves depend on the diameter and shape of the nanochannel and the bulk salt concentration. In the limit of wide channels, the apparent pKa?s and widths can be estimated by a simplified analytical model derived from the more general molecular theory. Both the general and the simplified theory predicts that, due to charge-regulation effects, the first acid?base transition (0\/?1 transition) is wider than the second one (&#8722;1\/?2), and both transitions are wider than the ideal one expected for an isolated acid?base group in the bulk. It is also shown that the inflection points of the conductance versus pH curves provide a very good estimation of the apparent pKa?s of the polyelectrolyte for cylindrical channels, but the quality of the estimation decreases for noncylindrical geometries.","palabras_clave":"Solid-state nanopores|Polymer brushes|Ionic rectification|Acid-Base Equilibrium"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5663,"idioma_id":4,"titulo":"Recognition of movements through dynamic electromyographic signals","resumen":"The recognition of movements through electromyographic (EMG) signals is critical for myoelectric control systems. Performance of these systems depend on processing methods  and protocols used to extract the EMG signals. The aim of this study is to evaluate the performance of classification of a kinematic recognition system based on dynamic EMG signals. For this, a correlation analysis between dynamic EMG signals and kinematic features of movements is realized, and then, a kinematic recognition system based on dynamic EMG signals is implemented. Dynamic EMG signals from forearm muscles during finger flexion movements were recorded and analyzed by using an amplitude estimator. Linear and no-linear correlations between EMG amplitudes and kinematic features were found. Then, a step of classification based on discriminant analysis was implemented to categorize the finger movements in multiple kinematic states. The accuracy of classifications were 95%, 88%, 81% and 76% for two, three, four and five states respectively, and by using a simple-channel recording and an EMG amplitude estimator. The results of this study demonstrate that it is possible to improve aspects of ?intuitiveness? through dynamic EMG evoked by natural and more intuitive movements.","palabras_clave":"EMG|root mean square|kinematic features|discriminant analysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5664,"idioma_id":4,"titulo":"Organocatalytic route for the synthesis of propionylated starch","resumen":"In recent years acetylated,propionylated and butyrylated starches have received special attention due to their capacity to deliver speci&#64257;c short chain fattyacids (SCFAs) to the colon in a sustained and predictable manner, and thus contribute to maintaining the normal physiologic function of the large bowel and preventing speci&#64257;c diseases. In the current contribution a non-conventional organocatalytic solventless route for the eco-friendly propionylation of corn starch is proposed. The catalyst used inthe acylation is a naturally occurring hydroxy acid (l-tartaric acid). Propionylated starches with degree of substitution (DS) in the 0.05-1.59 interval were obtained and characterized in terms of chemical structure, morphology, crystallinity, thermal stability and hydrophilicity. Results showed that by the proposed methodology propionylated starch with the DS requiredfor clinical use (i.e. 0.2-0.3) could be obtained within 2-3 h of reaction. Characterization results evidenced the progressive loss of crystallinity of starch granules as higher substitution levels","palabras_clave":"STARCH |PROPIONYLATION |HUMAN COLON HEALTH|ORGANOCATALYTIC SOLVENTLESS ROUTE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5665,"idioma_id":5,"titulo":"Cambios en la partición de flujos de agua en el Chaco Árido al reemplazar bosques por pasturas","resumen":"La vegetación cumple un papel muy importante regulando los flujos de agua de los ecosistemas, especialmente en regiones áridas y semiáridas donde la mayor parte de la precipitación es evapotranspirada. En este trabajo se exploró cómo el reemplazo de los bosques secos nativos por pasturas, orientado a aumentar la productividad ganadera, afecta la partición de flujos de agua en las llanuras del Chaco Árido. Se caracterizó la distribución de tamaño e intensidad de eventos de precipitación y se midieron los flujos de intercepción directa, escurrimiento superficial, drenaje profundo y evaporación potencial en parcelas pareadas de bosques y pasturas en San Luis, Argentina. Las pasturas presentaron menor intercepción directa (0,35 ± 0,13 vs. 1,51 ± 0,50 mm\/evento de precipitación), mayor escurrimiento superficial (~28 vs. 0% de escurrimiento en eventos grandes e intensos) y mayor evaporación potencial a nivel del suelo (7,0 ± 0,9 vs. 4,4 ± 0,9 mm\/día). El drenaje profundo resultó nulo en ambas coberturas, aunque bajo las pasturas se encontró un desplazamiento vertical de las sales del perfil hasta los 2 metros de profundidad, no registrado en el bosque. Luego de cada evento de precipitación, las pasturas presentaron una distribución más homogénea de la humedad edáfica como producto de una menor redistribución de agua por el canopeo y la superficie del terreno. Estos resultados sugieren que el establecimiento de pasturas, en sitios originalmente cubiertos por bosques, reduciría la fracción de la precipitación potencialmente transpirable y aumentaría el riesgo potencial de erosión hídrica. La importancia que los flujos de redistribución tienen sobre el balance hídrico y el funcionamiento ecohidrológico del sistema, resalta la necesidad de caracterizar mejor la dinámica del agua a escala de parche en estos paisajes llanos","palabras_clave":"BALANCE DE AGUA|CHACO SECO|DESMONTE|GANADERÍA EXTENSIVA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5666,"idioma_id":4,"titulo":"Effects of Methotrexate on Vascular Inflammation in a Rat Model of Metabolic Syndrome","resumen":"BACKGROUND: In this study, we used methotrexate (Mtx) to examine the role of immunomodulation on the activation of IL-6 and VCAM-1, which could generate a microenvironment that supports cardiovascular remodelling.METHODS: Male WKY and SHR rats were separated into five groups: Control, FFR: WKY rats receiving a 10% (w\/v) fructose solution during all 12 weeks, SHR, FFHR: SHR receiving a 10% (w\/v) fructose solution during all 12 weeks and FFHR+Mtx (0,3 mg\/kg intraperitoneal one injection per day week for 6 weeks) (n=8 per group). Metabolic variables and systolic blood pressure were measured. Cardiac and vascular remodelling was also evaluated. To assess this, IL-6R and VCAM-1 immunostaining techniques were used.RESULTS: The FFHR experimental model developed metabolic syndrome, vascular and cardiac remodelling, and vascular inflammation because of increased expression of IL-6 and VCAM-1. Chronic treatment with Mtx completely or partiality reversed the variables studied.CONCLUSIONS: The results demonstrated an impact on immunomodulation after mtx treatment, which included a reduction in vascular inflammation and a favourable reduction in metabolic and structural parameters.","palabras_clave":"citoquinas|remodelado vascular|inflamación vascular|Sindrome Metabolico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5667,"idioma_id":4,"titulo":"Effects of the presence of nutrients in the removal of high concentrations of Cr (III) by Typha domingensis","resumen":"The aim of this work was to study the influence of nutrients on the tolerance, removal efficiency and accumulation of high concentration of Cr(III) by Typha domingensis. This species was exposed to the following Cr(III) treatments, arranged in triplicate: 200 mg L&#8722;1 Cr; 600 mg L&#8722;1 Cr; 200 mg L&#8722;1 Cr + 50 mg L&#8722;1 P + 50 mg L&#8722;1 N; 600 mg L&#8722;1 Cr + 50 mg L&#8722;1 P + 50 mg L&#8722;1 N; without metal or nutrient additions (control 1); 50 mg L&#8722;1 P + 50 mg L&#8722;1 N, without metal addition (control 2). In order to simulate extreme events, the concentrations studied were higher than the concentrations commonly found in constructed wetlands. Cr and nutrient concentrations in water decreased in all treatments along time. Nutrient addition did not affect Cr removal for the two concentrations studied. In tissues, the highest Cr concentrations were observed in roots in all treatments. The mass balance showed that sediment showed the highest accumulation of Cr. Metals caused growth inhibition and a decrease in chlorophyll concentration. Despite these sublethal effects, T. domingensis demonstrated that it could accumulate Cr. These results confirm the high tolerance of this species ensuring the survival plant and wetland efficiency in time.","palabras_clave":"NUTRIENTS|METALS|MACROPHYTES|EFFLUENTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5668,"idioma_id":5,"titulo":"Modelo de acumulación y evolución secuencial del intervalo cuspidal de la Formación Quintuco en su área tipo: Implicancias para las reconstrucciones paleogeográficas del margen austral de la cuenca Neuquina durante el Valanginiano","resumen":"La Formación Quintuco (Berriasiano-Valanginiano temprano) aflorante en el centro de la provincia de Neuquén comprende depósitos marinos, transicionales y continentales. Estos depósitos fueron incluidos en diversos trabajos estratigráficos regionales, recibiendo tan sólo una interpretación paleoambiental  generalizada. La comprensión del estadio evolutivo correspondiente al tramo cuspidal de la Formación Quintuco, y su comparación con los depósitos basales de la Formación Mulichinco, es clave para estimar la magnitud del cambio asociado al evento de reconfiguración de cuenca ocurrido durante el Valanginiano temprano más tardío. Con el objetivo de reconstruir los últimos estadíos evolutivos de la Formación Quintuco, se presenta un análisis facial, arquitectural y secuencial del intervalo cuspidal de la unidad que aflora en la Sierra de la Vaca Muerta (sector centro-sur de la Cuenca Neuquina). El estudio consistió en el análisis sedimentológico de perfiles de detalle en combinación con el estudio arquitectural de intervalos claves. Con posterioridad, se analizó la distribución espacial de las diferentes asociaciones de facies reconocidas y se identificaron superficies claves que representan cambios significativos en los ambientes de acumulación. En este contexto, se abordó el análisis secuencial del intervalo estudiado y se discuten patrones de apilamiento de las sucesiones reconocidas y la evolución vertical de dicho intervalo. Este análisis permitió la identificación de 7 asociaciones de facies que representan la acumulación en subambientes de prodelta (AF 1), frente deltaico (AF 2 y AF 3), planicie deltaica distal (AF 4 y AF 5) y planicie deltaica proximal (AF 6 y AF 7).  La Formación Quintuco habría evolucionado en sus estadios finales como un sistema deltaico mixto, caracterizado por un dominio de los procesos fluviales y una influencia de olas en los sectores laterales a la zona de descarga. Los sectores proximales del sistema se localizaban hacia el SO, mientras que los sectores distales se distribuían hacia el E. El sistema deltaico se construyó mediante el apilamiento vertical de sucesiones somerizantes (15-40 m) que se delimitan por superficies transgresivas de gran escala. Estas sucesiones comprenden diferentes estadios de evolución del sistema de acumulación y se apilan verticalmente con un patrón progradacional de largo término. La configuración paleogeográfica para los últimos estadios evolutivos de la Formación Quintuco indica un área fuente localizada hacia el O-SO de la Sierra de la Vaca Muerta. Esta reconstrucción constituye una nueva contribución para la comprensión de las configuraciones paleogeográficas que tuvieron lugar en el margen austral de la cuenca durante el Valanginiano.","palabras_clave":"CUENCA NEUQUINA|FORMACIÓN QUINTUCO|VALANGINIANO TEMPRANO|SISTEMA DELTAICO MIXTO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5670,"idioma_id":4,"titulo":"A new Approach for Time Series Forecasting: Bayesian Enhanced by Fractional Brownian Motion with Application to Rainfall Series","resumen":"A new predictor algorithm based on Bayesian enhanced approach (BEA) for long-term chaotic time series using artificial neural networks (ANN) is presented. The technique based on stochastic models uses Bayesian inference by means of Fractional Brownian Motion as model data and Beta model as prior information. However, the need of experimental data for specifying and estimating causal models has not changed. Indeed, Bayes method provides another way to incorporate prior knowledge in forecasting models; the simplest representations of prior knowledge in forecasting models are hard to beat in many forecasting situations, either because prior knowledge is insufficient to improve on models or because prior knowledge leads to the conclusion that the situation is stable.This work contributes with long-term time series prediction, to give forecast horizons up to 18 steps ahead. Thus, the forecasted values and validation data are presented by solutions of benchmark chaotic series such as Mackey-Glass, Lorenz, Henon, Logistic, Rössler, Ikeda, Quadratic one-dimensional map series and monthly cumulative rainfall collected from Despeñaderos, Cordoba, Argentina. The computational results are evaluated against several non-linear ANN predictors proposed before on high roughness series that shows a better performance of Bayesian Enhanced approach in long-term forecasting.","palabras_clave":"long-term prediction|neural networks|Bayesian inference|Fractional Brownian Motion|Hurst parameter"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5671,"idioma_id":4,"titulo":"Prediction of Non-Genotoxic Carcinogenicity Based on Genetic Profiles of Short Term Exposure Assays","resumen":"Non-genotoxic carcinogens are substances that induce tumorigenesis by non-mutagenic mechanisms andlong term rodent bioassays are required to identify them. Recent studies have shown that transcription profilingcan be applied to develop early identifiers for long term phenotypes. In this study, we used rat liverexpression profiles from the NTP (National Toxicology Program, Research Triangle Park, USA) DrugMatrixDatabase to construct a gene classifier that can distinguish between non-genotoxic carcinogens and otherchemicals. The model was based on short term exposure assays (3 days) and the training was limited to oxidativestressors, peroxisome proliferators and hormone modulators. Validation of the predictor was performedon independent toxicogenomic data (TG-GATEs, Toxicogenomics Project-Genomics AssistedToxicity Evaluation System, Osaka, Japan). To build our model we performed Random Forests together witha recursive elimination algorithm (VarSelRF). Gene set enrichment analysis was employed for functionalinterpretation. A total of 770 microarrays comprising 96 different compounds were analyzed and a predictorof 54 genes was built. Prediction accuracy was 0.85 in the training set, 0.87 in the test set and increased withincreasing concentration in the validation set: 0.6 at low dose, 0.7 at medium doses and 0.81 at high doses.Pathway analysis revealed gene prominence of cellular respiration, energy production and lipoprotein metabolism.The biggest target of toxicogenomics is accurately predict the toxicity of unknown drugs. In this analysis,we presented a classifier that can predict non-genotoxic carcinogenicity by using short term exposureassays. In this approach, dose level is critical when evaluating chemicals at early time points.","palabras_clave":"toxicología|carcinogenicidad|perfiles genéticos|líneas celulares"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5672,"idioma_id":4,"titulo":"EGFR participates downstream of ER&#945; in estradiol-17ß-D-glucuronide induced impairment of Abcc2 function in isolated rat hepatocyte couplets","resumen":"Estradiol-17&#946;-D-glucuronide (E17G) induces acute endocytic internalization of canalicular transporters, including multidrug resistance-associated protein 2 (Abcc2) in rat, generating cholestasis. Several proteins organized in at least two different signaling pathways are involved in E17G cholestasis: one pathway involves estrogen receptor alpha (ER&#945;), Ca2+-dependent protein kinase C and p38-mitogen activated protein kinase, and the other pathway involves GPR30, PKA, phosphoinositide 3-kinase\/AKT and extracellular signal-related kinase 1\/2. EGF receptor (EGFR) can potentially participate in both pathways since it interacts with GPR30 and ER&#945;. Hence, the aim of this study was to analyze the potential role of this receptor and its downstream effectors, members of the Src family kinases in E17G-induced cholestasis. In vitro, EGFR inhibition by Tyrphostin (Tyr), Cl-387785 or its knockdown with siRNA strongly prevented E17G-induced impairment of Abcc2 function and localization. Activation of EGFR was necessary but not sufficient to impair the canalicular transporter function, whereas the simultaneous activation of EGFR and GPR30 could impair Abcc2 transport. The protection of Tyr was not additive to that produced by the ER&#945; inhibitor ICI neither with that produced by Src kinase inhibitors, suggesting that EGFR shared the signaling pathway of ER&#945; and Src. Further analysis of ER&#945;, EGFR and Src activations induced by E17G, demonstrated that ER&#945; activation precedes that of EGFR and EGFR activation precedes that of Src. In conclusion, activation of EGFR is a key factor in the alteration of canalicular transporter function and localization induced by E17G and it occurs before that&nbsp;of Src and after&nbsp;that of ER&#945;.","palabras_clave":"Mrp2|Src kinase|Cholestasis|ABC transporters"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5673,"idioma_id":4,"titulo":"Fasciola hepatica infection in humans: overcoming problems for the diagnosis","resumen":"Fascioliasis is a zoonosis actually considered as a foodborne trematode disease priority by the World Health Organization. Our study presents three cases of F. hepatica infection diagnosed by direct, indirect and\/or imaging diagnostic techniques, showing the need of the combined use of them. In order to overcome some difficulties of the presently available methods we show for the first time the application of molecular tools to improve human fascioliasis diagnosis by employing a PCR protocol based on a repetitive element as target sequence. In conclusion, diagnosis of human fascioliasis has to be carried out by the combination of diagnostic techniques that allow the detection of infection in different disease phases, different epidemiological situations and known\/new transmission patterns in the actual scenario.","palabras_clave":"Fascioliasis|enzyme-linked immunosorbent assay| polymerase chain reaction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5674,"idioma_id":4,"titulo":"Mullite (3Al2O3.2SiO2) ceramics obtained by reaction sintering of rice husk ash and alumina, phase evolution, sintering and microstructure","resumen":"The use of industrial waste (by-products) as raw materials in the ceramicindustry has been under study for decades due to the economical, energy, taxand environmental advantages. The specificity of the waste requires a basiccharacterization and technology thereof.The applicability of rice husk ash (RHA), as silica (SiO2) source, in refractory andporous materials with potential structural, insulating and\/or filteringapplications was carried out by characterizing the ceramic behavior ofstoichiometric mixtures of calcined alumina (Al2O3) and RHA. A reactionsinteringframework can be defined in the (Al2O3-SiO2) system. The sinterabilityand conversion during the reaction sintering processes were studied in order toobtain mullite (3Al2O3.2SiO2) ceramics. Also some microstructural features ofthe developed materials were studied in the 1100°-1600°C range. Themullitization was studied quantitatively.Partial densification was achieved (30%) and highly converted materials wereobtained. The developed microstructure consisted in a dense ceramic matrixwith homogenous interconnected porosity, with a narrow pore size distributionbelow 20 &#956;m. The developed material gives enough information for designingmullite ceramics materials with either porous or dense microstructures withstructural, insulating or filtering applications employing RHA as silica sourceand calcined alumina as the only other raw materia","palabras_clave":"mullite|rice husk|sintering"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5675,"idioma_id":5,"titulo":"Primera aproximación al estudio de fuentes secundarias de materias primas (área intercuencas río Avilés - río Chico, Tierra del Fuego, Argentina)","resumen":"En esta contribución se presentan los primeros resultados de un muestreo dirigido que permitió caracterizar cuantitativa y cualitativamente las potenciales fuentes secundarias de materias primas líticas utilizadas para la manufactura de artefactos en el sitio arqueológico Herradura 1. Este sitio se localiza en el noreste de Tierra del Fuego y fue ocupado por grupos cazadores-recolectores durante el Holoceno tardío. Para describir y comparar las fuentes secundarias se seleccionaron unidades de análisis donde las materias primas -que consisten en clastos sueltos- estuvieran dispuestas sobre la superficie. Los clastos fueron caracterizados de acuerdo con su génesis, tamaño de grano, homogeneidad del tamaño de grano, presencia de rasgos planares y número de fracturas. Los resultados obtenidos permiten afirmar que existen numerosas fuentes secundarias inmediatamente disponibles que contienen rocas de calidad irregular. En contraste, el análisis de los artefactos líticos recuperados en Herradura 1 indica una selección de materias primas de buena calidad para la talla, ya que se utilizaron rocas de grano fino, con tamaños de grano homogéneos y un bajo porcentaje de fracturas y rasgos planares.","palabras_clave":"TIERRA DEL FUEGO|LATE HOLOCENE| LITHIC RAW MATERIALS|SECONDARY LITHIC DEPOSITS|HUNTER-GATHERERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5676,"idioma_id":4,"titulo":"Coproparasitological study of European starling nestlings (Sturnus vulgaris) in Argentina","resumen":"We examined 71 fecal samples of European starling nestlings from Buenos Aires Province, Argentina, in the reproductive seasons of 2012 and 2013 to detect the presence of intestinal parasites and determine their prevalence. Total parasite prevalence was 26.8%. We found one coccidial species (Isospora sp.) with a prevalence of 25.35% and one cestode species with a prevalence of 1.4%.These values are lower than those reported for starlings from Europe and North America. Our results suggest that a reduction in the diversity of parasite species was likely to have occurred during the introduction process of this bird in Argentina, thus contributing to its invasive success.","palabras_clave":"Argentina|cestode|Isospora|starling nestling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5677,"idioma_id":4,"titulo":"The cardiac electrogenic sodium\/bicarbonate cotransporter (NBCe1) is activated by aldosterone through the G protein-coupled receptor 30 (GPR 30)","resumen":"The sodium\/bicarbonate cotransporter (NBC) transports extracellular Na+ and HCO3- into the cytoplasm upon intracellular acidosis, restoring the acidic pHi to near neutral values. Two different NBC isoforms have been described in the heart, the electroneutral NBCn1 (1Na+:1HCO3-) and the electrogenic NBCe1 (1Na+:2HCO3-). Certain non-genomic effects of aldosterone (Ald) were due toan orphan G protein-couple receptor 30 (GPR30). We have recently demonstrated that Ald activates GPR30 in adult rat ventricular myocytes, which transactivates the epidermal growth factor receptor (EGFR) and in turn triggers a reactive oxygen species (ROS)- and PI3K\/AKTdependent pathway, leading to the stimulation of NBC. The aim of this study was to investigate the NBC isoform involved in the Ald\/GPR30-induced NBC activation. Using specific NBCe1 inhibitory antibodies (a-L3) we demonstrated that Ald does not affect NBCn1 activity. Ald was ableto increase NBCe1 activity recorded in isolation. Using immunofluorescence and confocal microscopy analysis we showed in this work that both NBCe1 and GPR30 are localized in t-tubules. In conclusion, we have demonstrated that NBCe1 is the NBC isoform activated by Ald in the heart.","palabras_clave":"ELECTROGENIC SODIUM\/ BICARBONATE COTRANSPORTER|ALDOSTERONE|GPR30"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5678,"idioma_id":4,"titulo":"Multiple abiotic stresses on maize grain yield determination: additive vs multiplicative effects","resumen":"xxx","palabras_clave":"maize|multiple abiotic stresses|reproductive development|kernel number"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5679,"idioma_id":4,"titulo":"Multiscale model of the human cardiovascular system: description of heart failure and comparison of contractility indices","resumen":"AbstractA multiscale model of the cardiovascular system is presented. Hemodynamics is described by a lumped parameter model, while heart contraction is described at the cellular scale. An electrophysiological model and a mechanical model were coupled and adjusted so that the pressure and volume of both ventricles are linked to the force and length of a half-sarcomere. Particular attention was paid to the extremal values of the sarcomere length, which must keep physiological values. This model is able to reproduce healthy behavior, preload variations experiments, and ventricular failure. It also allows to compare the relevance of standard cardiac contractility indices. In this work we show that the theoreticalgold standard for assessing cardiac contractility, namely the end-systolic elastance, is actually load-dependent and therefore not a reliable index of cardiac contractility.","palabras_clave":"Mathematical models|Physiological models|Multiscale models|Cardiovascular system"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5680,"idioma_id":4,"titulo":"Simultaneous Study of Cholesterol and GM1 Ganglioside by Specific Probes: Lipid Distribution during Maturation, Capacitation and the Acrosome Reaction","resumen":"Lipid distribution has been extensively studied in the plasma membrane of the male gamete due to its relevantrole in sperm physiology. However, there are controversial reports about their distribution. Because of differentreports about this topic, the aim of this work was define cholesterol and GM1 ganglioside location by simultaneousdetection during sperm maturation, capacitation and the acrosome reaction in CF1 mouse sperm. We observed thatthroughout the epididymis, cholesterol detected by filipin III or complex and GM1 ganglioside by cholera toxin, colocalizedin the acrosomal domain of the head and midpiece of the flagellum. During sperm capacitation, a decreaseof cholesterol can be observed like diffuse pattern on the whole head, while GM1 ganglioside was restricted to theapical acrosome. Capacitated sperm incubated with acrosome reaction inducers showed a diffuse location onthe head with both probes after acrosomal exocytosis. Also, both probes did not show different locations on theflagellum. These findings indicate that cholesterol and GM1 ganglioside have a dynamic behaviour during the lifeof the sperm, which could indicate that a continuous assembly or disassembly of the lipid raft could be involved inthe responsiveness of the sperm to different environments. Thus, during sperm capacitation and acrosome reactionthere is a redistribution of the lipids. However, there are not apparent changes during sperm maturation. Cholesterolefflux, a characteristic event of sperm capacitation, could be analyzed through diffuse pattern described by filipin,and GM1 ganglioside patterns observed by the cholera toxin could be interesting when it is needed to evaluatesperm capacitation and the acrosome reaction in fixed sperm.","palabras_clave":"cholesterol|GM1 ganglioside|lipid|spermatozoa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5681,"idioma_id":4,"titulo":"Comparative study of biological activity of fluorinated 5- aminopyrazoles on Spodoptera frugiperda","resumen":"Several derivatives aminopyrazoles, which are a new class of synthetic insecticide agents, have shownbiological activity against lepidopteran, such as Spodoptera frugiperda. The aim of this work was to assess the biologicalactivity of these synthesized compounds. For this purpose, derivatives of 5-amino-1-aryl-1H-pyrazole-4-carbonitriles weresynthesized from commercially available aryl hydrazines and (ethoxymethylene)malononitrile. Three compounds (A, B, C)were tested for in vitro biological activity; two of them, A [5-amino-1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-1H-pyrazole-4-carbonitrile] and B [5-amino-1-(perfluorophenyl)-1H-pyrazole-4-carbonitrile] showed promising activity, particularly thecompound A.","palabras_clave":"AMINO PIRAZOLES|INSECTICIDAS|SPODOPTERA FRUGIPERDA|POLYPHAGOUS LEPIDOPTERAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5682,"idioma_id":4,"titulo":"A simple and inexpensive method for evaluating the photovoltaic potential. Its validation in Buenos Aires and Antarctica","resumen":"The use of renewable energies requires a precise and detailed quantification of the resource available. Because of the cost of solar stations or limited availability of skilled human resources, in most emerging countries, this assessment is made only on a few points scattered over large areas. We report here a simple and inexpensive method to evaluate the photovoltaic (PV) potential for a specific geographic region and a given PV capture technology. The system allows for a direct evaluation of the energy actually obtainable by scaling the measurement array of photovoltaic cells. The proposed measurement system can be installed as a stand-alone unit, or as part of a measurement network, connected to a more sophisticated central hub. The measurement station consists of said PV array (or similar PV array), a resistor, and a portable data logger. The system is calibrated with a device composed of a small array of PV cells, a resistor load bank, and two multimeters. Due to its low cost, this system can be replicated as many times as required with minimal investment. This would make it possible to evaluate the available photovoltaic potential of large regions with accurate and detailed data. Measurements carried out in Buenos Aires and in Antarctica confirm the consistency of the method.","palabras_clave":"Solar Energy|Photovoltaic potential"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5683,"idioma_id":4,"titulo":"Sensing electroadsorption reactions and surface mobility of electroadsorbed species by scanning electrochemical induced desorption","resumen":"A theoretical analysis of electro-adsorption reactions and of the surface diffusion of electro-adsorbed species based on scanning electrochemical microscopy (SECM) in the feedback mode, usually known as scanning electrochemical induced desorption (SECMID), is presented. Numerical simulations of the classical feedback process were carried out by including in the model a potential-dependent electro-adsorption reaction from the mediator at the substrate and allowing the adsorbed species (Aad) to diffuse over the substrate surface affecting the mediator loop. As in classical SECMID, the local variation of the mediator concentration underneath the tip causes a potential-dependent gradient of the Aad surface coverage at the substrate over the tip-affected region, which drives the Aad surface diffusion toward this area and the consequent positive feedback of mediator, reaching a steady state. The simulated steady-state dependences of the tip current (iT) on the substrate potential (ES) show the presence of a peak over the potential range affected by the electro-adsorption reaction, whose amplitude at a given tip-substrate distance is mostly influenced by the surface diffusion coefficient of Aad and the density of adsorption sites at the substrate. When this surface process is parallel to an electrode reaction of the mediator that proceeds over the same potential range, the adsorption\/diffusion peak is overlapped with the typical Butler-Volmer type response of the electrode reaction, affecting the iT(ES) shape and interfering with the determination of kinetic parameters from this dependence. These phenomena were experimentally observed when using the H+\/H2 mediator loop on Au and Pt, where Had is electro-adsorbed from H+, a process that in the case of Pt is parallel to the oxidation of the tip generated H2.","palabras_clave":"SECM|ELECTRO-ADSORPTION|SURFACE DIFFUSION|HYDROGEN ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5684,"idioma_id":4,"titulo":"Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2","resumen":"Addition of methyl groups to arginine residues is catalyzed by a group of enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 is essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported to be expressed in nervous system and involved in neurogenesis. In this work, we found that Prmt8 is expressed in mouse embryonic stem cells (ESC) and in induced pluripotent stem cells, and modulated along differentiation to neural precursor cells. We found that Prmt8 promoter activity is induced by the pluripotency transcription factors Oct4, Sox2 and Nanog. Moreover, endogenous Prmt8 mRNA levels were reduced in ESC transfected with Sox2 shRNA vector. As a whole, our results indicate that Prmt8 is expressed in pluripotent stem cells and its transcription is modulated by pluripotency transcription factors. These findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells.","palabras_clave":"Prmt8|celulas madre pluripotentes|Sox2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5685,"idioma_id":4,"titulo":"Generation of iPSC line iPSC-FH2. 1 in hypoxic conditions from human foreskin fibroblasts","resumen":"Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.","palabras_clave":"iPSC|hESC|HYPOXIA|REPROGAMMING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5687,"idioma_id":4,"titulo":"A new Late Cretaceous crocodyliform from the western margin of Gondwana (La Rioja Province, Argentina)","resumen":"TheCretaceous witnessed the greatest diversity of Gondwanan notosuchian crocodyliforms,which displayed high levels of diversity and a notable array of specializedforms that developed in different ecological niches. Among this diversity, theadvanced notosuchians are a clade of mid body sized forms which displays aremarkable abundance but is restricted to two lithological units from the LateCretaceous of South America, the Adamantina Formation in southeastern Braziland the Bajo de la Carpa Formation in Patagonia (Argentina). The onlyexceptions known so far were the Late Cretaceous Labidiosuchus from the MariliaFormation in Brazil and Yacarerani from the Cajones Formation in Bolivia. Hereinwe report a new Cretaceous crocodyliform, Llanosuchus tamaensis gen. nov. etsp. nov., found in the Los Llanos Formation (Campanian?) in northwesternArgentina (La Rioja Province). The small specimenincludes well preserved fragments of the cranium and mandible with an estimatedskull size of about 9 cm lengths. This crocodyliform shares several derivedcharacters with Notosuchus terrestris from the Bajo de la Carpa Formation(Patagonia) and it was found in a weakly developed sandy paleosol profile formedin wet local conditions but in a region characterized by semi-arid climate andactive eolian sedimentation. The presence of a new advanced notosuchian in the Cretaceousof western Gondwana, and its intermediate geographical region between the knownSouth American species (Brazil, Argentina and Bolivia), has interestingimplications, and adds another record of an advanced notosuchian in deposits withinferred warm climates and semi-arid paleoenvironments. Finally, Llanosuchustamaensis supports a Late Cretaceous age for Los Llanos Formation withimportant geological implications for La Rioja Province.","palabras_clave":"Crocodyliform|Notosuchia|La Rioja|Cretaceous"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5689,"idioma_id":4,"titulo":"Microbial ecology involved in the ripening of naturally fermented llama meat sausages. A focus on lactobacilli diversity","resumen":"Llama represents for the Andean regions a valid alternative to bovine and pork meat and thanks to the high proteins and low fat content; it can constitute a good product for the novel food market. In this study, culture-dependentand independent methods were applied to investigate the microbial ecology of naturally fermented llama sausages produced in Northwest Argentina. Two different production technologies of llama sausage were investigated:a pilot-plant scale (P) and an artisanal one (A). Results obtained by High-Throughput Sequencing (HTS) of 16S rRNA amplicons showed that the production technologies influenced the development of microbial communities with a different composition throughout the entire fermentation process. Both sequencing and microbiological counts demonstrated that Lactic Acid Bacteria (LAB) contributed largely to the dominant microbiota. When a total of 230 isolates were approached by RAPD-PCR, presumptive LAB strains from P production exhibited an initial variability in RAPD fingerprints switching to a single profile at the final of ripening,while A production revealed a more heterogeneous RAPD pattern during the whole fermentation process. The constant presence of Lactobacillus sakei along the fermentation in both productions was revealed by HTS and confirmed by species specific PCR from isolated strains. The technological characterization of Lb. sakei isolates evidenced their ability togrow at 15 °C, pH 4.5 and 5% NaCl (95%). Most strains hydrolyzed myofibrillar and sarcoplasmic proteins. Bacteriocins encoding genes and antimicrobial resistance were found in 35% and 42.5% of the strains, respectively. Anappropriate choice of a combination of autochthonous strains in a starter formulation is fundamental to improve and standardize llama sausages safety and quality.","palabras_clave":"LLAMA MEAT|FERMENTED SAUSAGES|HIGH-THROUGHPUT SEQUENCING |RAPD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5690,"idioma_id":4,"titulo":"Genetic Characterization and Pathogenicity of Rhizoctonia solani Associated with Common Bean Web Blight in the Main Bean Growing area of Argentina","resumen":"Common bean web blight (WB), caused by the fungus Rhizoctonia solani (teleomorph Thanatephorus cucumeris), is among the endemic fungal diseases of major impact in north-western Argentina (NWA). This study aimed to analyse the genetic and pathogenic diversity of R. solani in Salta, NWA, where 97 isolates were recovered from commercial bean cultivars and wild beans showing WB symptoms in a major bean production area. The isolates were characterized on the basis of specific primers, rDNA-ITS sequences and morphological characteristics. All the isolates were identified as R. solani AG 2-2WB, and they exhibited considerable intragroup variation. The phylogenetic tree generated with the ITS sequences confirmed the isolates identification. Aggressiveness of the isolates towards bean seedlings was assessed in the greenhouse. A great variability in virulence was observed among the isolates analysed. On the basis of the disease reaction on foliar tissues, the isolates were grouped into three virulence categories as follows: weakly virulent (30%), moderately virulent (38%) and highly virulent (32%). However, no correlation between virulence and geographical origin was detected. The information generatedin this study provides initial data on the population variability of the WB pathogen in north-western Argentina and represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.","palabras_clave":"Rhizoctonia solani|genetic diversity|Phaseolus vulgaris|virulence|ITS|sequencing|morphology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5691,"idioma_id":4,"titulo":"Vaginal microbial communities from synchronized-heifers and cows with reproductive disorders","resumen":"Aim: To evaluate changes in the resident microbial population in the cranial vaginal mucosa induced by a a progesterone-releasing intravaginal device (PRID) compared to the vaginal microbiota of cows with reproductive disorders.Methods and Results: Vaginal discharge was evaluated by clinical examination and a Vaginitis Diagnosis Score was performed by exfoliative cytology. All samples classified as positive and some classified as negative by clinical evaluation were later diagnosed as positive for vaginitis by cytological analysis. Bacterial diversity profiles were performed by PCR-DGGE and clustered according to the reproductive health status of the specimens, revealing a correspondence between the structures of the communities in the vagina and the clinical profile. Representative bands from each group were sequenced and identified as Ruminococcus sp., Dialister sp., Escherichia sp.\/Shigella sp., Virgibacillus sp., Campylobacter sp., Helcoccoccus sp., Staphylococcus sp., Bacillus sp., Actinopolymorpha sp., Exiguobacterium sp., Haemophilus sp.\/Histophilus sp., Aeribacillus sp., Porphyromonas sp., Lactobacillus sp. and Clostridium sp.Conclusion: Our results contribute to the knowledge of the vaginal microbiome in synchronized heifers showing positive or negative clinical vaginitis.Significance and Impact of Study: This study contributes to the understanding of a dynamic vaginal colonization by bacterial consortiums during the synchronization with a widely used PRID-protocol. Also, the results reveal the presence of well-known metritis-related pathogens as well as emerging uterine opportunistic pathogens. The provided information will allow to carry out further studies to elucidate functional roles of these native microorganisms in the bovine reproductive tract.Keywords: vaginal microbiota, post-PRID vaginitis, metritis, cows, heifers","palabras_clave":"VAGINAL MICROBIOTA|COWS|POST-PRID VAGINITIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5692,"idioma_id":4,"titulo":"New insights into the atmospheric mercury cycling in Central Antarctica and implications at a continental scale","resumen":"Under the framework of the GMOS project (Global Mercury Observation System) atmospheric mercury monitoring has been implemented at Concordia Station on the high-altitude Antarctic plateau (75°06? S, 123°20? E, 3220 m above sea level). We report here the first year-round measurements of gaseous elemental mercury (Hg(0)) in the atmosphere and in snowpack interstitial air on the East Antarctic ice sheet. This unique dataset shows evidence of a continuous oxidation of atmospheric Hg(0) in summer (24-hour daylight) due to the high oxidative capacity of the Antarctic plateau atmosphere at this period of the year. Summertime Hg(0) concentrations exhibited a pronounced daily cycle in ambient air with maximal concentrations around midday. Photochemical reactions and chemical exchange at the air\/snow interface were prominent, highlighting the role of the snowpack on the atmospheric mercury cycle. Our observations reveal a 20 to 30 % decrease of atmospheric Hg(0) concentrations from May to mid-August (winter, 24-h darkness). This phenomenon has never been observed elsewhere and likely results from a gas-phase oxidation and\/or heterogeneous reactions. We also reveal the occurrence of multi-day to weeklong atmospheric Hg(0) depletion events in summer, not associated with depletions of ozone, and likely due to a stagnation of air masses above the plateau triggering an accumulation of oxidants within the shallow boundary layer. Our observations suggest that the inland atmospheric reservoir is depleted in Hg(0) in summer. Due to katabatic winds flowing out from the Antarctic plateau down the steep vertical drops along the coast and according to observations at coastal Antarctic stations, the striking reactivity observed on the plateau most likely influences the cycle of atmospheric mercury at a continental scale.","palabras_clave":"Atmospheric Mercury|Central Antarctica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5693,"idioma_id":4,"titulo":"A Simple Three Step Method for Selective Placement of Organic Groups in Mesoporous Silica Thin Films","resumen":"Selective functionalization of mesoporous silica thin films was achieved using a three step method. The first step consists in an outer surface functionalization, followed by washing off the structuring agent (second step), leaving the inner surface of the pores free to be functionalized in the third step. This reproducible method permits to anchor a volatile silane group in the outer film surface, and a second type of silane group in the inner surface of the pores. As a concept test we modified the outer surface of a mesoporous silica film with trimethylsilane (eSie(CH3)3) groups and the inner pore surface with propylamino (eSie(CH2)3eNH2) groups. The obtained silica films were characterized by Environmental Ellipsometric Porosimetry (EEP), EDS, XPS, contact angle and electron microscopy. The selectively functionalized silica (SF) shows an amount of surface amino functions 4.3 times lower than the one-step functionalized (OSF) silica samples. The method presented here can be extended to a combination of silane chlorides and alkoxides as functional groups, opening up a new route toward the synthesis of multifunctional mesoporous thin films with precisely localized organic functions.","palabras_clave":"mesoporous thin films"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5694,"idioma_id":4,"titulo":"Chemical cycling and deposition of atmospheric mercury in Polar Regions: review of recent measurements and comparison with models","resumen":"Mercury (Hg) is a worldwide contaminant that can cause adverse health effects to wildlife and humans. While atmospheric modeling traces the link from emissions to deposition of Hg onto environmental surfaces, large uncertainties arise from our incomplete understanding of atmospheric processes (oxidation pathways, deposition, and reemission). Atmospheric Hg reactivity is exacerbated in high latitudes and there is still much to be learned from Polar Regions in terms of atmospheric processes. This paper provides a synthesis of the atmospheric Hg monitoring data available in recent years (2011?2015) in the Arctic and in Antarctica along with a comparison of these observations with numerical simulations using four cutting-edge global models. The cycle of atmospheric Hg in the Arctic and in Antarctica presents both similarities and differences. Coastal sites in the two regions are both influenced by springtime atmospheric Hg depletion events and by summertime snowpack reemission and oceanic evasion of Hg. The cycle of atmospheric Hg differs between the two regions primarily because of their different geography. While Arctic sites are significantly influenced by Northern Hemispheric Hg emissions especially in winter, coastal Antarctic sites are significantly influenced by the reactivity observed on the East Antarctic ice sheet due to katabatic winds. Based on the comparison of multi-model simulations with observations, this paper discusses whether the processes that affect atmospheric Hg seasonality and inter-annual variability are appropriately represented in the models, and identifies research gaps in our understanding of the atmospheric Hg cycling in high latitudes","palabras_clave":"Atmospheric Mercury|Polar REgions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5695,"idioma_id":4,"titulo":"Differential physiological responses of two Salvinia species to hexavalent chromium at a glance","resumen":"n plants of Salvinia rotundifolia and Salvinia minima the effect of two Cr(VI) concentrations (5 and 20 mg L&#8722;1) applied for 7 days was assessed by measuring changes in biomass, photosynthetic pigments, Cr accumulation, malondialdehyde (MDA), membrane stability index (MSI), thiols (TT, NPT and PBT), and phenolics (SP and IP). Biomass in S. minima was decreased at highest Cr(VI) concentration, but there were no changes in S. rotundifolia. Metal accumulation was different in both species. S. minima accumulates more metal in fronds, but S. rotundifolia accumulates more metal in lacinias. Results also showed that S. minima translocates more Cr to fronds than S. rotundifolia, but at the whole plant level higher accumulation occurred in this last. Tolerance index (Ti) was higher in S. rotundifolia. Chl b and carotenoids were decreased only upon exposure to high Cr(VI) concentration in both species. Cr(VI) treatment did not enhance MDA accumulation. Cr exposure had no impact on MSI values when comparing with Cr-untreated values. Thiols in fronds and lacinias showed different distribution patterns between species. IP and NPT were higher in S. rotundifolia lacinias that accumulate more Cr than S. minima lacinias. Whilst SP and NPT were higher in S. minima fronds compared with S. rotundifolia ones. This may indicate that these species can cope with Cr(VI) toxicity, either through metal complexation and\/or metal reduction or by the scavenging of ROS derived from Cr-induced oxidative stress. Based on Cr accumulation and biomass production, S. rotundifolia seems more suitable to remove Cr(VI) from polluted waters.","palabras_clave":"CHROMIUM ACCUMULATION|SALVINIA SPECIES|PHENOLIC|TOLERANCE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5696,"idioma_id":5,"titulo":"Efecto del entrenamiento con un juego de estrategia en tiempo real sobre la toma de decisiones en adolescentes","resumen":"Se evaluó si el entrenamiento con un videojuego de estrategia en tiempo real podía mejorar la toma de decisiones (TD) en adolescentes de ambos sexos de 15 a 18 años de la ciudad de Córdoba (Argentina). Se utilizó un diseño experimental con pre y postest con grupo control. El grupo experimental pasó por 7 sesiones de entrenamiento de 40 minutos con el videojuego Dark Reign (variable independiente). Debido a que los datos no presentaron una distribución normal, se usaron pruebas estadísticas no paramétricas. Para la medición de la TD se utilizaron diversas pruebas. En primer lugar, el análisis del rendimiento global en la Iowa Gambling Task sólo reveló un efecto principal del tratamiento. Por otro lado, al analizar el rendimiento por bloques, los varones del grupo experimental mejoraron su puntuación en los bloques 3 y 4. No se encontraron diferencias en el rendimiento en las pruebas Cognitive Bias Task y Game of Dice Task. La mejora del grupo experimental para el puntaje global de la IGT, acompañado con la mejora encontrada en varones del grupo experimental para el puntaje por bloques de la IGT, sugiere que el tratamiento tuvo un efecto sobre la capacidad para tomar decisiones ventajosas, destacando el beneficio a largo plazo antes que las recompensas inmediatas en este grupo.","palabras_clave":"TOMA DE DECISIONES|ADOLESCENTES|VIDEOJUEGO|DARK REIGN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5697,"idioma_id":4,"titulo":"Monocyte-derived dendritic cells early exposed to Mycobacterium tuberculosis induce an enhanced T helper 17 response and transfer mycobacterial antigens","resumen":"Tuberculosis (TB) is a complex disease, and the success of the bacterium depends on its ability to evade the immune response. Previously, we determined that Mycobacterium tuberculosis (Mtb) impairs the function of dendritic cells (DC),promoting the generation of cells that are poor stimulators of mycobacterial antigen-specific CD4T cells, which are required to control this persistent infection. In this study, we aimed to determine the mechanisms by which monocyte-derived DCs differentiated in the presence of Mtb (MtbDC) may impact on the proliferation of specific anti-mycobacterial T cells. We found that the presence of Mtb during monocyte-derived DC differentiation favours T helper (Th) 2 and Th17 polarization, in detriment of a Th1 response, compared to DC mature with Mtb. The bias on T cell polarization was associated to the profile of C-type lectin receptors expression found in MtbDC (DC-SIGNlow\/MRlow\/Dectin-1high).Alternatively, MtbDC release Mtb antigens (Ag) that can be taken up and resented by bystander DC, promoting the proliferation of CD4T cells, but to a lesser extent than direct presentation by Mtb-matured DC. In summary, we have furthercharacterized the generation of MtbDC as an effective evasion strategy driven by the pathogen, leading to the inhibition of Ag-presentation and bias of T cell polarization towards Th2 and Th17 profiles, features which partially explain thepersistence of Mtb in the host.","palabras_clave":"ANTIGEN TRANSFER|DENDRITIC CELLS|MYCOBACTERIUM TUBERCULOSIS|TUBERCULOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5698,"idioma_id":4,"titulo":"Decreased Ox-LDL uptake and cholesterol efflux in THP1 cells elicited by cortisol and by cortisone through 11&#946;-hydroxysteroid dehydrogenase type 1","resumen":"Background and aims: Data about glucocorticoids role in the development of atherosclerosis arecontroversial showing different effects in human than in experimental animal models. Atherosclerosis isthe result of a chronic inflammatory response to an injured endothelium where an uncontrolled uptakeof OxLDL by macrophages triggers the development of foam cells, the main component of fatty streaks inatherosclerotic plaque. There are few data about the direct effect of glucocorticoids in macrophages ofatherosclerotic plaque. The aim of the study was to elucidate the role of glucocorticoids in the devel-opment of foam cells in atherosclerosis initiation.Methods: For this purpose we used THP1 cells differentiated to macrophages with phorbol esters andincubated with OxLDL alone or with cortisol or cortisone. THP1 cells were also incubated with cortisoneplus an inhibitor of 11 b -hydroxysteroid dehydrogenase 1 (11 b HSD1) activity to determine the role of thisenzyme on glucocorticoid action in this process.Results: Ours results showed that cortisol and cortisone decreased significantly the inflammation pro-moted by OxLDL, and also diminished the expression of genes involved in influx and efflux of cholesterolresulting in a reduced lipid accumulation. Likewise cortisol and cortisone decreased 11 b HSD1 expressionin THP1 cells. The presence of the inhibitor of 11 b HSD1 abolished all the effects elicited by cortisone.Conclusion: Our results indicate a direct effect of glucocorticoids on macrophages braking atherosclerosisinitiation, reducing pro-inflammatory markers and OxLDL uptake and cholesterol re-esterification, butalso inhibiting cholesterol output. These effects appear to be mediated, at least in part, by 11 b HSD1activity.","palabras_clave":"CORTISOL|CORTISONE|11&#946;-HYDROXYSTEROID DEHYDROGENASE 1|11&#946;-HYDROXYSTEROID DEHYDROGENASE 2|THP1 MACROPHAGES|FOAM CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5699,"idioma_id":4,"titulo":"Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell&ndash;cell contacts and filopodia-induced zippering in prostate cancer","resumen":"Prostate cancer (PCa) cells display abnormal expression of cytoskeletal proteins resulting in an augmented capacity to resistchemotherapy and colonize distant organs. We have previously shown that heme oxygenase 1 (HO-1) is implicated in cellmorphology regulation in PCa. Here, through a multi ´omics´ approach we define the HO-1 interactome in PCa, identifying HO-1molecular partners associated with the integrity of the cellular cytoskeleton. The bioinformatics screening for these cytoskeletalrelatedpartners reveal that they are highly misregulated in prostate adenocarcinoma compared with normal prostate tissue. UnderHO-1 induction, PCa cells present reduced frequency in migration events, trajectory and cell velocity and, a significant higherproportion of filopodia-like protrusions favoring zippering among neighboring cells. Moreover forced expression of HO-1 was alsocapable of altering cell protrusions in transwell co-culture systems of PCa cells with MC3T3 cells (pre-osteoblastic cell line).Accordingly, these effects were reversed under siHO. Transcriptomics profiling evidenced significant modulation of key markersrelated to cell adhesion and cell?cell communication under HO-1 induction. The integration from our omics-based researchprovides a four molecular pathway foundation (ANXA2\/HMGA1\/POU3F1; NFRSF13\/GSN; TMOD3\/RAI14\/VWF; and PLAT\/PLAU)behind HO-1 regulation of tumor cytoskeletal cell compartments. The complementary proteomics and transcriptomics approachespresented here promise to move us closer to unravel the molecular framework underpinning HO-1 involvement in the modulationof cytoskeleton pathways, pushing toward a less aggressive phenotype in PCa.","palabras_clave":"Heme oxygenase-1|prostate|microscopy|filopodia-like protrusion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5700,"idioma_id":4,"titulo":"Data related to inflammation and cholesterol deposition triggered by macrophages exposition to modified LDL","resumen":"This article supports experimental evidence on the time-dependent effect on gene expression related to inflammation and cholesterol deposition in lipid-loaded cells. The cells employed were human monocytes THP1 line transformed into macrophages by treatment with phorbol esters. Macrophages were treated at different times with oxidized low density lipoprotein (Ox-LDL) and then gene expression was measured. We also include data about the different types of oxidized lipoprotein obtained (low, media or high oxidation) for differential exposure with Cu ions. These data include characterization to lipid and protein peroxidative damage and also quantification of cell viability by exposure to native and modified LDL. The present article complements data published in ?Decreased OxLDL uptake and cholesterol efflux in THP1 cells elicited by cortisol and by cortisone through 11&#946;-hydroxysteroid dehydrogenase type 1?","palabras_clave":"Foam cells|THP1 macrophages|Oxidized LDL|Atherosclerosis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5701,"idioma_id":4,"titulo":"Role of foliar fungal endophytes on litter decomposition among species and population origins","resumen":"Litter decomposition is a keyecosystem process which returns nutrients from dead plant material to mineralforms in the soil. We examined whether systemic fungal endophytes modulaterecycling of nutrients directly by altering litter decomposition. We studied litterdecomposition mediated by Epichloë endophytes in litter-bag experiments. Weexamined direct endophyte effects on litter decomposition in wild populationsand cultivars of Schedonorus phoenix and Schedonorus pratensis. In the firstexperiment, endophyte presence tended to increase litter decomposition rate incultivars of the two grass species (S. phoenix and S. pratensis). However, inthe second experiment plant origin had a stronger influence than endophytesymbiosis in S. phoenix. Interestingly, the initial level of alkaloids wasassociated positively with decomposition in S. phoenix populations.Characteristics associated with litter quality were not clearly related toeither endophytes or decomposition rate. Our results suggest that endophytescan enhance litter breakdown but their role in nutrient cycling is far morecomplex depending on plant population origin. ","palabras_clave":"Epichloë |Festuca spp.|Symbiosis|Plantemicroorganism interaction|Ecosystem processes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5702,"idioma_id":4,"titulo":"Toxin distribution and sphingoid base imbalances in Fusarium verticillioides-infected and fumonisin B1-watered maize seedlings","resumen":"Fusarium verticillioides is a major maize pathogen and there are susceptible andresistant cultivars to this fungal infection. Recent studies suggest that its main mycotoxin fumonisin B1 (FB1) may be involved in phytopathogenicity, but theunderlying mechanisms are mostly still unknown. This work was aimed at assessingwhether FB1 disseminates inside the plants, as well as identifying possible correlations between the maize resistant\/susceptible phenotype and the unbalances of the FB1-structurally-related sphingoid base sphinganine (Sa) and phytosphingosine (Pso) due to toxin accumulation. Resistant (RH) and susceptible hybrid (SH) maize seedlings grown from seeds inoculated with a FB1-producer F. verticillioides and from uninoculated ones irrigated with FB1 (20 ppm), were harvested at 7, 14 and 21 days after planting (dap), and the FB1, Sa and Pso levels were quantified in roots and aerial parts. The toxin was detected in roots and aerial parts for inoculated and FB1-irrigated plants of both hybrids. However, FB1 levels were overall higher in SH seedlings regardless of thetreatment (infection or watering). Sa levels increased substantially in RH lines, peaking at 54 fold in infected roots at 14 dap. In contrast, the main change observed in SH seedlings was an increase of Pso in infected roots at 7 dap. Here, it was found that FB1 disseminates inside seedlings in the absence of FB1-producer fungal infections, perhaps indicating this might condition the fungus-plant interaction before the first contact.Furthermore, the results strongly suggest the existence of at least two ceramide synthase isoforms in maize with different substrate specificities, whose differential expression after FB1 exposure could be closely related to the susceptibility\/resistance to F. verticillioides.","palabras_clave":"Fusarium verticillioides|Maize|Zea mays L.|Poaceae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5703,"idioma_id":4,"titulo":"Arabidopsis proline dehydrogenase contributes to flagellin mediated PAMP-triggered immunity by affecting RBOHD","resumen":"Plants activate different defense systems to counteract the attack of microbial pathogens. Among them, the recognition of conserved microbial\/pathogen associated molecular patterns (MAMPs\/PAMPs) by pattern-recognition receptors stimulates MAMP\/PAMP-triggered immunity (MTI\/PTI). In recent years, the elicitors, receptors and signaling pathways leading to PTI have been extensively studied. However, the contribution of organelles to this program deserves further characterization. Here, we studied how processes altering the mitochondrial electron transport chain (mETC) influence PTI establishment. With particular emphasis we evaluated the effect of proline (Pro) dehydrogenase (ProDH), an enzyme that can load electrons into the mETC and regulate the cellular redox state. We found that mETC uncouplers (antimycin\/rotenone) and MnSOD deficiency impair flg22-induced responses, such as accumulation of reactive oxygen species (ROS) and bacterial growth limitation. ProDH mutants also reduce these defenses, decreasing as well callose deposition. Using ProDH inhibitors and ProDH inducers (exogenous Pro treatment) we showed that this enzyme modulates the generation of ROS by the plasma membrane respiratory burst NADPH oxidase homolog D (RBOHD). In this way, we contribute to the understanding of mitochondrial activities influencing early and late PTI responses, and the coordination of the redox-associated mitochondrial enzyme ProDH with defense events initiated at the plasma membrane.","palabras_clave":"PTI|flg22|ProDH|ROS|mitochondria|RBOHD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5704,"idioma_id":4,"titulo":"Clomipramine and Benznidazole act synergistically and ameliorate the outcome of experimental Chagas disease: In vivo and in vitro assessments","resumen":"Chagas disease is an important public health problem in Latin America, and its treatment by chemotherapy with benznidazole (BZ) or nifurtimox remains unsatisfactory. In order to design new alternative strategies to improve the current etiological treatments, in the present work, we comprehensively evaluated the in vitro and in vivo anti-Trypanosoma cruzi effects of clomipramine (CMP) (a parasite-trypanothione reductase-specific inhibitor) combined with BZ. In vitro studies, carried out using a checkerboard technique on trypomastigotes (T. cruzi strain Tulahuen), revealed a combination index (CI) of 0.375, indicative of a synergistic effect of the drug combination. This result was correlated with the data obtained in infected BALB\/c mice. We observed that during the acute phase (15 days postinfection [dpi]), BZ at 25 mg\/kg of body weight\/day alone decreased the levels of parasitemia compared with those of the control group, but when BZ was administered with CMP, the drug combination completely suppressed the parasitemia due to the observed synergistic effect. Furthermore, in the chronic phase (90 dpi), mice treated with both drugs showed less heart damage as assessed by the histopathological analysis, index of myocardial inflammation, and levels of heart injury biochemical markers than mice treated with BZ alone at the reference dose (100 mg\/kg\/day). Collectively, these data support the notion that CMP combined with low doses of BZ diminishes cardiac damage and inflammation during the chronic phase of cardiomyopathy. The synergistic activity of BZ-CMP clearly suggests a potential drug combination for Chagas disease treatment, which would allow a reduction of the effective dose of BZ and an increase in therapeutic safety.","palabras_clave":"Chagas disease therapy|Trypanosoma cruzi|benznidazole|clomipramine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5705,"idioma_id":4,"titulo":"Chronic Trypanosoma cruzi infection potentiates adipose tissue macrophage polarization toward an anti-inflammatory M2 phenotype and contributes to diabetes progression in a diet-induced obesity model","resumen":"Chronic obesity and Chagas disease (caused by the protozoan Trypanosoma cruzi) represent serious public health concerns. The interrelation between parasite infection, adipose tissue, immune system and metabolism in an obesogenic context, has not been entirely explored. A novel diet-induced obesity model (DIO) was developed in C57BL\/6 wild type mice to examine the effect of chronic infection (DIO+I) on metabolic parameters and on obesity-related disorders. Dyslipidemia, hyperleptinemia, and cardiac\/hepatic steatosis were strongly developed in DIO mice. Strikingly, although these metabolic alterations were collectively improved by infection, plasmatic apoB100 levels remain significantly increased in DIO+I, suggesting the presence of pro-atherogenic small and dense LDL particles. Moreover, acute insulin resistance followed by chronic hyperglycemia with hypoinsulinemia was found, evidencing an infection-related-diabetes progression. These lipid and glucose metabolic changes seemed to be highly dependent on TLR4 expression since TLR4-\/- mice were protected from obesity and its complications. Notably, chronic infection promoted a strong increase in MCP-1 producing macrophages with a M2 (F4\/80+CD11c-CD206+) phenotype associated to oxidative stress in visceral adipose tissue of DIO+I mice. Importantly, infection reduced lipid content but intensified inflammatory infiltrates in target tissues. Thus, parasite persistence in an obesogenic environment and the resulting host immunometabolic dysregulation may contribute to diabetes\/atherosclerosis progression.","palabras_clave":"OBESITY|INMUNOMETABOLISM|ADIPOSE TISSUE MACROPHAGES|INNATE IMMUNITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5706,"idioma_id":4,"titulo":"CD73 Inhibition Shifts Cardiac Macrophage Polarization toward a Microbicidal Phenotype and Ameliorates the Outcome of Experimental Chagas Cardiomyopathy","resumen":"Increasing evidence demonstrates that generation of extracellular adenosine fromATP, which is hydrolyzed by theCD39\/CD73 enzyme pair, attenuates the inflammatory response and deactivates macrophage antimicrobial mechanisms. Although CD73 is emerging as a critical pathway and therapeutic target in cardiovascular disorders, the involvement of this ectonucleotidase during myocardial infection has not been explored. Using a murine model of infection with Trypanosoma cruzi, the causal agent of Chagas cardiomyopathy, we observed a sudden switch from the classical M1 macrophage (microbicidal) phenotype toward an alternative M2 (repairing\/antiinflammatory) phenotype that occurred within the myocardium very shortly after BALB\/c mice infection. The observed shift inM1\/M2 rate correlated with the cardiac cytokine milieu. Considering that parasite persistence within myocardium is a necessary and sufficient condition for the development of the chronic myocarditis, we hypothesized that CD73 activity may counteract cardiac macrophage microbicidal polarization, rendering the local immune response less effective. In fact, a transient treatment with a specific CD73 inhibitor (adenosine 59-a,b-methylene-diphosphate) enhanced the microbicidal M1 subset predominance, diminished IL-4-and IL-10-producing CD4+ T cells, promoted a proinflammatory cytokine milieu, and reduced parasite load within the myocardium during the acute phase. As a direct consequence of these events, there was a reduction in serum levels of creatine kinase muscle-brain isoenzyme, a myocardial-specific injury marker, and an improvement in the electrocardiographic characteristics during the chronic phase. Our results demonstrate that this purinergic system drives the myocardial immune response postinfection and harbors a promising potential as a therapeutic target.","palabras_clave":"CD73|Chagas Cardiomyopathy.|Cardiac Macrophage"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5708,"idioma_id":4,"titulo":"Differential contribution of malic enzymes during soybean and castor seeds maturation","resumen":"This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malic enzymes (ME) catalyze the decarboxylation of malate generating pyruvate, CO2 and NADH or NADPH. In some organisms it has been established that ME is involved in lipids biosynthesis supplying carbon skeletons and reducing power. In this work we studied the MEs of soybean and castor, metabolically different oilseeds. The comparison of enzymatic activities, transcript profiles and organic acid contents suggest different metabolic strategies operating in soybean embryo and castor endosperm in order to generate precursors for lipid biosynthesis. In castor, the malate accumulation pattern agrees with a central role of this metabolite in the provision of carbon to plastids, where the biosynthesis of fatty acids occurs. In this regard, the genome of castor possesses a single gene encoding a putative plastidic NADP-ME, whose expression level is high when lipid deposition is active. On the other hand, NAD-ME showed an important contribution to the maturation of soybean embryos, perhaps driving the carbon relocation from mitochondria to plastids to support the fatty acids synthesis in the last stages of seed filling. These findings provide new insights into intermediary metabolism in oilseeds and provide new biotechnological targets to improve oil yields.","palabras_clave":"MALIC ENZYME|SOYBEAN|CASTOR |LIPIDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5709,"idioma_id":4,"titulo":"Early ovarian follicular development in prepubertal Wistar rats acutely exposed to androgens","resumen":"Androgens may directly modulate early ovarian follicular development in preantral stages and androgen excess before puberty may disrupt this physiological process. Therefore, the aim of this study was to investigate the dynamics of follicular morphology and circulating androgen and estradiol levels in prepubertal Wistar rats acutely exposed to androgens. Prepubertal female Wistar rats were distributed into three groups: control, equine chorionic gonadotropin (eCG) intervention and eCG plus dehydroepiandrosterone (DHEA) intervention (eCG+DHEA). Serum DHEA, testosterone and estradiol levels were determined, and ovarian morphology and morphometry were assessed. The eCG+DHEA group presented increased serum estradiol and testosterone levels as compared with the control group (P&lt;0.01), and higher serum DHEA concentration v. the eCG-only and control groups (P&lt;0.01). In addition, the eCG+DHEA group had a higher number of, and larger-sized, primary and secondary follicles as compared with the control group (P&lt;0.05). The eCG group presented intermediate values for number and size of primary and secondary follicles, without significant differences as compared with the other two groups. The number of antral follicles was higher in the eCG+DHEA and eCG groups v. controls (P&lt;0.05). The number of primordial, atretic and cystic follicles were similar in all groups. In conclusion, the present experimental model using an acute eCG+DHEA intervention was useful to investigate events involved in initial follicular development under hyperandrogenic conditions, and could provide a reliable tool to study defective follicular development with possible deleterious reproductive consequences later in life.","palabras_clave":"SINDROME DE OVARIO POLIQUISTICO|HIPERANDROGENISMO|DESARROLLO FOLICULAR|ATRESIA FOLICULAR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5710,"idioma_id":4,"titulo":"Effect of preharvest solar radiation on apple skin oxidative disorders during cold storage","resumen":"Sunscald and superficial scald are physiological disorders of apple that develop during low temperature storage. Sunscald is exclusively observed on sun-exposed section, while superficial scald manifests itself on non-exposed skin. Malus × domestica, ´Granny Smith´ sun-exposed (ET) and non-exposed tissues (NET) were compared during 0, 90, 120, 150, and 180 days of cold-storage (DCS) in terms of sunscald and superficial scald incidence, &#945;-farnesene (AF) and conjugated trienes (CT), antioxidant activity (DPPH radical-scavenging), lipid peroxidation (thiobarbituric acid-reactive substances, TBARs) and maturity indices. After 90 DCS and 1 week at 20°C, sunscald was observed in ET and affected 41.4% of the fruit. Superficial scald was detected in NET and affected 66.5% of the fruit. At 180 DCS these disorders reached 100%. After long storage, AF concentration increased. The highest level (125.5 and 95.8 nmol cm-2 for ET and NET, respectively) was measured at 120 days of storage, but decreased at 180 days. CT in ET were lower during all storage period, but increased significantly in NET after 90 days in parallel with superficial scald symptoms. During all cold-storage period DPPH was significantly higher (P<0.05) in ET than NET. At harvest, ET showed significantly more TBARs (59.8 nmol&#8232;g-1 FW) than NET (43.8 nmol g-1 FW). During storage, lipid peroxidation increased and reached at 180 days 101.8 nmol g-1 FW in NET and 75.5 nmol g-1 FW in ET. There were significant differences in fruit maturity between ET and NET. Flesh firmness and soluble solid concentration were higher in ET than NET throughout the sampling period. These results showed that different physiological oxidative process may occur in exposed and non-exposed tissues during cold storage and the higher antioxidant activities found in sun-exposed tissue were ineffective in preventing sunscald.","palabras_clave":"Malus × domestica|antioxidants |sunscald|superficial scald|fruit quality"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5711,"idioma_id":4,"titulo":"Relationship between the Balance of Hypertrophic\/Hyperplastic Adipose Tissue Expansion and the Metabolic Profile in a High Glucocorticoids Model","resumen":"Abstract: Adipose tissue (AT) expansion is the result of two processes: hyperplasia and hypertrophy; and both, directly or indirectly, depend on the adipogenic potential of adipocyte precursor cells (APCs). Glucocorticoids (GCs) have a potent stimulatory effect on terminal adipogenesis; while their effects on early stages of adipogenesis are largely unknown. In the present work, we study, in a model of high GC levels, the adipogenic potential of APCs from retroperitoneal AT (RPAT) and its relationshipwithRPATmassexpansion. Weemployedamodelofhyper-adiposity(30-and60-day-old rats)duetohighendogenousGClevelsinducedbyneonataltreatmentwith L-monosodiumglutamate (MSG). We found that the RPAT APCs from 30-day-old MSG rats showed an increased adipogenic capacity, depending on the APCs? competency, but not in their number. Analyses of RPAT adipocyte diameter revealed an increase in cell size, regardless of the rat age, indicating the prevalence of a hypertrophicprocess. Moreover,functionalRPATalterationsworsenedin60-day-oldrats,suggesting that the hyperplastic AT expansion found in 30-day-old animals might have a protective role. We concludethatGCschronicexcessaffectsAPCs?adipogeniccapacity,modifyingtheircompetency. This change would modulate the hyperplastic\/hypertrophic balance determining healthy or unhealthy RPAT expansion and, therefore, its functionality.","palabras_clave":"retroperitoneal adipose tissue;\t\t\t\t|adipogenesis;|stromal vascular fraction cells;|cell determination;|adipogenic competency"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5712,"idioma_id":4,"titulo":"Aspergillus fumigatus intrinsic fluconazole resistance is due to the naturally occurring T301I substitution in Cyp51Ap","resumen":"Aspergillus fumigatus intrinsic fluconazole-resistance has been demonstrated to be linked to the CYP51A gene, although the precise molecular mechanism has not been elucidated yet. Comparisons between A. fumigatus Cyp51Ap and Candida albicans Erg11p sequences showed differences in amino acid residues already associated with fluconazole resistance in C. albicans. The aim of this study was to analyze the role of the natural polymorphism I301 in the Aspergillus fumigatus Cyp51Ap in the intrinsic fluconazole resistance phenotype of this pathogen. The I301 residue at the A. fumigatus Cyp51Ap was replaced with a threonine (analogue to T315 at Candida albicans fluconazole-susceptible Erg11p) by changing one single nucleotide in CYP51A gene. Also a CYP51A knock out strain was obtained using the same parental strain. Both mutant´s antifungal susceptibilities were tested. The I301T mutant exhibited a lower level of resistance to fluconazole (MIC 20 µg\/ml) than the parental strain (MIC 640 µg\/ml) while no changes in MIC values were observed for other azole and non-azole based drugs. This data strongly implicate the A. fumigatus Cyp51Ap I301 residue in the intrinsic resistance to fluconazole.","palabras_clave":"ASPERGILLUS FUMIGATUS|FLUCONAZOLE|RESISTANCE|AZOLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5713,"idioma_id":4,"titulo":"Ecological conditions and isotopic diet ( 13 C and 15 N) of Holocene caviomorph rodents in Northern Patagonia","resumen":"This work improves our knowledge about the relationship between diet and environment among Northern Patagonian caviomorph rodents. In order to characterize long-term individual diets, we present d13 C andd 15N data from osteological remains of the caviomorph rodentsDolichotis patagonum, Myocastorcoypus, Lagostomus maximus, Lagidium viscacia, Microcavia australis, Galea leucoblepharaandCtenomys sp., recovered from archaeological and recent contexts. These data are analyzed by temporal and geographic context and compared with the previous dietary studies based on macrorremains and microhistological analysis. Significative differences were found between rodents from Monte and Patagonian phytogeographic provinces, as well as between archaeological and modern members of the genus Ctenomys, indicating diet changes between Late Holocene and recent times individuals, that could confirm the climatic variations and\/or the recent overgrazing effect. The results indicate trends in rodent diets of 20th and 21st centuries were different to those of the Late Holocene, suggesting a decrease in precipitation and\/or an increase of the summer pattern of the same, and\/or an increase of mean annual temperature. In addition, this work confirms the generalist strategy of several taxa, although mainly in Ctenomys, which its diet co-varies with these environments","palabras_clave":"STALBE ISOTOPES |CAVIMORPH RODENTS|DIET|PALEODIET|MONTE DESERT| PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5714,"idioma_id":4,"titulo":"Evolutionary algorithm for metabolic pathways synthesis (IF 1.472)","resumen":"Metabolic pathway building is an active eld of research, necessary to understand and manipulate the metabolism of organisms. There are different approaches, mainly based on classical search methods, to nd linear sequences of reactions linking two compounds. However, an important limitation of these methods is the exponential increase of search trees when a large number of compounds and reactions is considered. Besides, such models do not take into account all substrates for each reaction during the search, leading to solutions that lack biological feasibility in many cases. This work proposes a new evolutionary algorithm that allows searching not only linear, but also branched metabolic pathways, formed by feasible reactions that relate multiple compounds simultaneously. Tests performed using several sets of reactions show that this algorithm is able to find feasible linear and branched metabolic pathways.","palabras_clave":"Evolutionary algorithms|search strategies|de novo pathway building|reactions network"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5715,"idioma_id":4,"titulo":"Superoxide and hydrogen peroxide productions by NO-inhibited complex III","resumen":"Complex III plays a central role in the mitochondrial respiratory chain transferring electrons from ubiquinol to cytochrome c and pumping protons to the intermembrane space, contributing to the protonmotive force. Furthermore, complex III can act as a source of O2?- in the presence of ubiquinol and antimycin, which blocks the oxidation of the cytochrome b hemes. The O2?- dismutation catalyzed by superoxide dismutase produces H2O2, a known second messenger in redox signalling. Results from our laboratory have shown that NO, released from GSNO or from SPER NO or generated by mtNOS, inhibits electron transfer at ubiquinone-cytochrome b area producing antimycin-like effects. Thus, both antimycin- and NO-inhibited complex III showed a high content of cytochromes b in the reduced state (79 and 71%, respectively) and an enhancement in the ubisemiquinone EPR signal at g=1.99 (42 and 35%, respectively). As consequence, O2?- and H2O2 productions were increased, being the O2?-\/H2O2 ratio equal to 1.98 in accordance with the stoichiometry of the O2?- disproportionation. The interruption of the oxidation of cytochromes b by NO leads to an enhancement of the steady-state concentration of UQH? allowing cytochrome bc1 complex to act as a source of reactive oxygen species in physiological conditions","palabras_clave":"S-NITROSOGLUTATHIONE|SPERMINE-NONOATE|ELECTRON PARAMAGNETIC RESONANCE|UBISEMIQUINONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5716,"idioma_id":4,"titulo":"Mitochondrial dysfunction in the diabetic heart","resumen":"Diabetic cardiomyopathy, i.e. the ventricular dysfunction in the absence of hypertension or coronary arterial disease, is a common complication of diabetes mellitus that leads to a heightened risk of heart failure and death among diabetic patients. This contractile dysfunction could be associated to mitochondrial dysfunction, in which mitochondrial biogenesis could emerge as a compensatory mechanism triggered in response to hyperglycemia. It has been proposed that nitric oxide synthase activities with enhanced NO production are involved in this process. In streptozotocin-induced diabetic rats, alterations in contractile response and lusitropic reserve, after &#946; adrenergic stimuli, were observed. Additionally, tissue O2 consumption was declined. A condition of mitochondrial dysfunction with decreased mitochondrial state 3 O2 consumption, respiratory control ratio, mitochondrial respiratory complexes activities and ATP production were present in hearts of diabetic animals. We observed an increase in mitochondrial NO production and in cytochrome oxidase activity measured in heart homogenates. This latter suggests an increase of new mitochondria. Thus, the impairment of mitochondrial function with enhancement in mitochondrial biogenesis could precede the onset of diabetic cardiomyopathy. However, mitochondrial biogenesis does not necessarily imply that resultant mitochondria are functional; this might explain the impairment in cardiac energy metabolism that occurs in hearts of diabetic rats","palabras_clave":"DIABETES|DIABETIC CARDIOMYOPATHY|HEART|MITOCHONDRIA|NITRIC OXIDE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5717,"idioma_id":4,"titulo":"An Algorithm for Prescribed Mean Curvature using Isogeometric Methods","resumen":"We present a Newton type algorithm to find parametric surfaces of prescribed mean curvature with a fixed given boundary. In particular, it applies to the problem of minimal surfaces. The algorithm relies on some global regularity of the spaces where it is posed, which is naturally fitted for discretization with isogeometric type of spaces. We introduce a discretization of the continuous algorithm and present a simple implementation using the recently released isogeometric software library igatools. Finally, we show several numerical experiments which highlight the convergence properties of the scheme.","palabras_clave":"minimal surfaces|prescribed curvature|isogeometric analysis|Quasi-Newton"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5718,"idioma_id":4,"titulo":"Programmed lab experiments for biochemical investigation of quorum-sensing signal molecules in rhizospheric soil bacteria","resumen":"Biochemistry courses in the Department of Molecular Biology at the National University of Río Cuarto, Argentina, are designed for undergraduate students in biology, microbiology, chemistry, agronomy, and veterinary medicine. Microbiology students typically have previous coursework in general, analytical, and organic chemistry. Programmed sequences of lab experiments allow these students to investigate biochemical problems whose solution is feasible within the context of their knowledge and experience. We previously designed and reported a programmed lab experiment that familiarizes microbiology students with techniques for detection and characterization of quorum-sensing (QS) and quorum-quenching (QQ) signal molecules. Here, we describe a sequence of experiments designed to expand the understanding and capabilities of biochemistry students using techniques for extraction and identification of QS and QQ signal molecules from peanut rhizospheric soil bacteria, including culturing and manipulation of bacteria under sterile conditions. The program provides students with an opportunity to perform useful assays, draw conclusions from their results, and discuss possible extensions of the study.","palabras_clave":"Quorum sensing|AHLs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5719,"idioma_id":4,"titulo":"Miraflores rocks in Tierra del Fuego (southernmost South America): Hunter-gatherer procurement, optimal pathway analysis and social interaction","resumen":"Using GIS applications to simulate optimal human movements from archaeological sites to the lithic source known as Miraflores Valley (Isla Grande de Tierra del Fuego, Southernmost South America), this paper discusses potential pathways for the spatial distribution of artifacts made of the two distinctive Miraflores raw materials (tuff and silicified tuff). In addition, we discuss possible models of exploitation and transport for Miraflores rocks through a distributional analysis on the depositional environment of the findings. Archaeological collections aswell as petrographic and geochemical studies showa large-scale archaeological distribution of Miraflores rocks; however they are absent from most Fuegian lithic assemblages and scarce in those wherein they appear.We propose and discuss the provisioning strategies applied in their procurement. Potential pathways through the islandscape are correlated with a statistical analysis based on the location of sites with the presence or absence of Miraflores raw materials. Spatial analyses provide a robust framework to discussMiraflores distribution and circulation, humanmobility aswell as the social interactionamong terrestrial hunter-gatherers and maritime populations of southernmost South America.Our results indicate that Miraflores rocks procurement varied across Fuegian geography.While independent circuits andmultiple accesses to the source may have existed in the north of Isla Grande de Tierra del Fuego, the distribution of these rocks southwards may have had a higher dependence on a few optimal routes connected to northern archaeological localities of San Sebastián and Inútil Bays. Therefore, southern populations may have drawn heavily on social interaction for the supply of Miraflores raw materials. Nevertheless, Least Cost Path model suggests that the participation of canoe people was not required to explain the current distribution of Miraflores materials throughout the Isla Grande de Tierra del Fuego.","palabras_clave":"GIS|SPATIAL DISTRIBUTION|MIRAFLORES ROCKS|HUNTER-GATHERER MOVILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5720,"idioma_id":5,"titulo":"Análisis paleodietario de individuos humanos procedentes del sitio arqueológico La Rinconada (Valle de Ambato, Catamarca","resumen":"Presentamos los primeros resultados de valores isotópicos (&#948;13Cco, &#948;13Cap y &#948;15N) realizados sobre piezas óseas y dentales de restos humanos hallados en La Rinconada (Valle de Ambato, Catamarca). Este sitio comprende una cronología que se extiende desde los ca. 600 DC hasta los 1200 DC, siendo el fin de su ocupación abrupta y repentina. La muestra aquí analizada corresponde a seis individuos, siendo el objetivo principal estimar las paleodietas humanas. Para la construcción del modelo de inferencia se presentan valores isotópicos de los recursos probablemente consumidos, incluyendo valores locales de Lama glama. Asimismo, se consideró que el sitio se emplaza en un ambiente ecotonal, y por lo tanto, con acceso a recursos de diferentes dominios fitogeográficos. Dado que tres de los individuos presentaron evidencias de haber sido afectados por procesos de termoalteración, se evaluó el efecto de dicha variable sobre las mediciones isotópicas y en consecuencia, su utilidad en la estimación paleodietaria. Hallamos que tanto el componente vegetal como animal de la dieta se enmarcan dentro de un patrón fotosintético C4. Asimismo, se encontró una escasa variación en los valores isotópicos al interior del conjunto.","palabras_clave":"Período de Integración Regional|isótopos estables|termoalteración|Zea mays"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5722,"idioma_id":5,"titulo":"Comparación de la respuesta antihipertensiva aguda al telmisartán y al irbesartán en ratas espontáneamente hipertensas","resumen":"Introducción: El telmisartán y el irbesartán, dos de los principales antagonistas del receptor AT1 disponibles para el controlde enfermedades cardiovasculares, difieren en sus propiedades farmacológicas, incluyendo el tiempo de disociación desde elreceptor AT1 y la capacidad de activar otros receptores, con potencial impacto en su eficacia clínica relativa.Objetivo: Comparar la respuesta cardiovascular aguda de la administración de una dosis única de irbesartán o de telmisartánen ratas espontáneamente hipertensas.Material y métodos: Se utilizaron 24 ratas espontáneamente hipertensas macho de 250-275 g, a las que se les canuló la arteriacarótida y la vena femoral para la medición directa de la presión arterial media (PAM) y la administración de irbesartán3-6 mg\/kg o de telmisartán 0,5-1 mg\/kg. Se estimó el cambio en la PAM, la frecuencia cardíaca y la variabilidad de la presiónarterial a corto plazo y latido-a-latido.Resultados: Aunque ambos antagonistas redujeron la PAM, el telmisartán indujo una respuesta antihipertensiva más prolongadaque el irbesartán, evidenciada por mayor reducción de la PAM luego de 180 minutos (-33,3% ± 4,1% vs. -16,3% ± 4%;p < 0,05). El telmisartán y el irbesartán atenuaron de manera prolongada la variabilidad de la presión arterial a corto plazo,sin diferencias entre ambos grupos experimentales. En el nivel de dosis más bajo, el telmisartán disminuyó en mayor medida lafrecuencia cardíaca y la variabilidad latido-a-latido en los diferentes dominios de frecuencia en comparación con el irbesartán.Conclusiones: En ratas espontáneamente hipertensas, la administración de telmisartán induce un efecto antihipertensivo másprolongado y una respuesta bradicardizante mayor que el irbesartán. El análisis espectral de la variabilidad latido-a-latidosugiere que el telmisartán, en la dosis más baja, atenúa en mayor medida la actividad simpática vascular en comparacióncon el irbesartán","palabras_clave":"telmisartan|irbesartan|hypertension"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5725,"idioma_id":4,"titulo":"Sustained-release hydrogels of topotecan for retinoblastoma","resumen":"Treatment of retinoblastoma, the most common primary ocular malignancy in children, has greatly improved over the last decade. Still, new devices for chemotherapy are needed to achieve better tumor control. The aim of this project was to develop an ocular drug delivery system for topotecan (TPT) loaded in biocompatible hydrogels of poly(&#949;-caprolactone)-poly(ethyleneglycol)-poly(&#949;-caprolactone) block copolymers (PCL-PEG-PCL) for sustained TPT release in the vitreous humor.Hydrogels were prepared from TPT and synthesized PCL-PEG-PCL copolymers. Rheological properties and in vitro and in vivo TPT release were studied. Hydrogel cytotoxicity was evaluated in retinoblastoma cells as a surrogate for efficacy and TPT vitreous pharmacokinetics and systemic as well as ocular toxicity were evaluated in rabbits. The pseudoplastic behavior of the hydrogels makes them suitable for intraocular administration. In vitro release profiles showed a sustained release of TPT from PCL-PEG-PCL up to 7 days and drug loading did not affect the release pattern. Blank hydrogels did not affect retinoblastoma cell viability but 0.4%(w\/w) TPT-loaded hydrogel was highly cytotoxic for at least 7 days. After intravitreal injection, TPT vitreous concentrations were sustained above the pharmacologically active concentration. One month after injection, animals with blank or TPT-loaded hydrogels showed no systemic toxicity or retinal impairment on fundus examination, electroretinographic, and histopathological assessments. These novel TPT-hydrogels can deliver sustained concentrations of active drug into the vitreous with excellent biocompatibility in vivo and pronounced cytotoxic activity in retinoblastoma cells and may become an additional strategy for intraocular retinoblastoma treatment.","palabras_clave":"TOPOTECAN|HYDROGELS|CYTOTOXICITY|RETINOBLASTOMA|RABBITS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5726,"idioma_id":5,"titulo":"Aplicación del modelo SCOR en PyMEs metalmecánicas de Olavarría","resumen":"El buen desempeño de la totalidad de la cadena de suministro (CS) es fundamental para cualquier industria y su medición permite conocer cuantitativamente su comportamiento, por lo que es necesario buscar enfoques innovadores que beneficien a todos sus actores. Una acertada opción es el modelo de referencia de operaciones de la CS (SCOR) diseñado por el Supply Chain Council. El mismo estandariza la terminología y los procesos de la CS usando indicadores de desempeño para comparar y analizar diferentes alternativas y estrategias de cada parte de la CS y del total de ella.En este sentido, el objetivo del presente trabajo es definir  las distintas partes que conformaran el modelo SCOR para pymes metalmecánicas de Olavarría, de modo de identificar la cadena de suministro y la categoría de cada proceso, como así también definir los indicadores y las mejores prácticas para cada configuración.","palabras_clave":"cadena de suministro|SCOR|PYMES industriales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5727,"idioma_id":4,"titulo":"Thyroid disorders and nitric oxide in cardiovascular adaptation to hypovolemia","resumen":"The aim of this study was to investigate whether nitric oxide participate in the cardiovascular function and haemodynamic adaptation to acute hemorrhage in animals with thyroid disorders. Sprague-Dawley rats aged 2 months old treated with T3 (Hyper, 20ug\/100g body weight) or 0.02% methimazole (hypo, w\/v) during 28 days were pretreated with NG nitro-L-arginine methyl ester (L-NAME) and submitted to 20% blood loss. Heart function was evaluated by echocardiography. Measurements of arterial blood pressure, heart rate, nitric oxide synthase activity and protein levels were performed. We found that hypo decreased fractional shortening and ejection fraction and increased left ventricle internal diameter. Hyper decreased ventricle diameter and no changes in cardiac contractility. Hemorrhage elicited a hypotension of similar magnitude within the 10 min. Then, this parameter was stabilized at about 30-40 min and maintained until finalized 120 min. L-NAME rats showed that the immediate hypotension would be independent of nitric oxide. Nitric oxide synthase inhibition blunted the changes of heart rate induced by blood loss. Hyper and hypo had lower atrial enzyme activity associated with a decreased enzyme isoforms in hypo. In ventricle, Hyper and hypo had a higher enzyme activity which was not correlated with changes in protein levels. Hemorrhage induced an increase heart nitric oxide production. We conclude that thyroid disorders were associated with hypertrophic remodelling which was and impacted differently on cardiac function and its adaptation to a hypovolemia. Hypovolemia triggered a nitric oxide synthase activation modulating the heart function to maintain haemodynamic homeostasis. This involvement depends on a specific enzyme isoform, cardiac chamber and thyroid state.","palabras_clave":"OXIDO NITRICO|HEMORRAGIA AGUDA|DESORDENES TIROIDEOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5728,"idioma_id":4,"titulo":"Modulation of expression and activity of intestinal multidrug resistance-associated protein 2 by xenobiotics","resumen":"The multidrug resistance-associated protein 2 (MRP2\/ABCC2) is a transporter that belongs to the ATP-binding cassette (ABC) superfamily. In the intestine, it is localized to the apical membrane of the enterocyte and plays a key role in limiting the absorption of xenobiotics incorporated orally. MRP2 may also play a role in systemic clearance of xenobiotics available from the serosal side of the intestine. MRP2 transports a wide range of substrates, mainly organic anions conjugated with glucuronic acid, glutathione and sulfate and its expression can be modulated by xenobiotics at transcriptional- and post-transcriptional levels. Transcriptional regulation is usually mediated by a group of nuclear receptors. The pregnane X receptor (PXR) is a major member of this group. Relevant drugs described to up-regulate intestinal MRP2 via PXR are rifampicin, spironolactone and carbamazepine, among others. The constitutive androstane receptor (CAR, NR1I3) was also reported to modulate MRP2 expression, phenobarbital being a typical activator. Dietary compounds, including micronutrients and other natural products, are also capable of regulating intestinal MRP2 expression transcriptionally. We have given them particular attention since the composition of the food ingested daily is not necessarily supervised and may result in interactions with therapeutic drugs. Post-transcriptional regulation of MRP2 activity by xenobiotics, e.g. as a consequence of inhibitory actions, is also described in this review. Unfortunately, only few studies report on drug-drug or nutrient-drug interactions as a consequence of modulation of intestinal MRP2 activity by xenobiotics. Future clinical studies are expected to identify additional interactions resulting in changes in efficacy or safety of therapeutic drugs.","palabras_clave":"MRP2|drug-drug-interaction|intestine|drug-transport"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5729,"idioma_id":4,"titulo":"Chromatographic fractionation and molecular mass characterization of Cercidium praecox (Brea) gum","resumen":"BACKGROUND: Brea gum (BG) is an exudate from the Cercidium praecox tree that grows in semi-arid regions of Argentina.Some previous studies on BG have shown physicochemical characteristics and functional features similar to those of gumarabic. However, there is a need to elucidate the molecular structure of BG to understand the functionality. In this sense, BGwas fractionated using hydrophobic interaction chromatography and the obtained fractions were analyzed by size exclusionchromatography.RESULTS: Analysis of the fractions showed that the bulk of the gum (approx. 84% of the polysaccharides) was a polysaccharideof 2.79 × 10 3 kDa. The second major fraction (approx. 16% of the polysaccharides) was a polysaccharide?protein complex witha molecular mass of 1.92 × 10 5 kDa. A third fraction consisted of protein species with a wide range of molecular weights. Themolecular weight distribution of the protein fraction was analyzed by size exclusion chromatography. Comparison of the elutionprofiles of the exudates in native and reducing conditions revealed that some of the proteins were forming aggregates throughdisulfide bridges in native conditions. Further analysis of the protein fraction by SDS-PAGE showed proteins with molecularweight ranging from 6.5 to 66 kDa.CONCLUSIONS: The findings showed that BG consists of several fractions with heterogeneous chemical composition andpolydisperse molecular weight distributions.","palabras_clave":"Cercidium praecox |Brea gum|plant gum exudate|molecular mass distribution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5730,"idioma_id":4,"titulo":"Complexes between ovalbumin nanoparticles and linoleic acid: Stoichiometric, kinetic and thermodynamic aspects","resumen":"Stoichiometric, kinetic and thermodynamic aspects of complex formation between heat-induced aggregates of ovalbumin (ovalbumin nanoparticles, OVAn) and linoleic acid (LA) were evaluated. Extrinsic fluorescence data were fitted to modified Scatchard model yielding the following results: n: 49 ± 2 LA molecules bound per OVA monomer unit and Ka: 9.80 ± 2.53 x 105 M. Kinetic and thermodynamic properties were analyzed by turbidity measurements at different LA\/OVA monomer molar ratios (21.5-172) and temperatures (20-40°C). An adsorption approach was used and a pseudo-second-order kinetics was found for LA-OVAn complex formation. This adsorption process took place within 1 hour. Thermodynamic parameters indicated that LA adsorption on OVAn was a spontaneous, endothermic and entropically-driven process, highlighting the hydrophobic nature of the LA and OVAn interaction. Finally, Atomic Force Microscopy imaging revealed that both OVAn and LA-OVAn complexes have a roughly rounded form with size lower than 100 nm.","palabras_clave":"LINOLENIC ACID|OVALBUMIN NANOPARTICLES|KINETICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5731,"idioma_id":5,"titulo":"Nanotechnology offers a promising therapeutic approach for hypertension treatment","resumen":"Hypertension is a medical condition considered one of the most important public health problems in developed countries, affecting around one billion people. Therefore, a priority is the study of mechanisms, development and treatment. Of particular interest are multiple contributing factors, and also are important efforts by experts to fully understand it. However, studies are currently insufficient and accordingly, attention is focused on the exploration of new therapeutic approaches. This raises a growing interest in nanotechnology given the ability of certain structures to mimic the behavior of extracellular matrices. This open a promising field in the treatment of diseases such as hypertension, where it stands to tissue engineering and its potential applications incorporating concepts such as controlled release drug, reduced side effects and receptor activation locally.","palabras_clave":"NANOTECNOLOGÍA|NANOTÚBULOS|ANANDAMIDA|HIPERTENSIÓN ARTERIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5733,"idioma_id":4,"titulo":"Effects of wortmannin on cardioprotection exerted by ischemic preconditioning in rat hearts subjected to ischemia-reperfusion","resumen":"Ischemic preconditioning (IPC) is one of the most powerful interventions to reduce ischemia-reperfusion injury. The aim of the present study was to investigate the involvement of the phosphatidylinositol-3-kinases (PI3Ks) family in cardioprotection exerted by IPC and the relationship between preservation of mitochondrial morphology and ATP synthesis capacity. In this regard, macroautophagy (autophagy) is considered a dynamic process involved in the replacement of aged or defective organelles under physiological conditions. IPC consisted of four 5-min cycles of ischemia-reperfusion followed by sustained ischemia. Wortmannin (W), a PI3K family inhibitor, was added to the perfusion medium to study the involvement of autophagy in the beneficial effects of IPC. In the present study, LC3-II\/I expression was significantly increased in the IPC group when compared with the control group. The hearts subjected to IPC showed greater degradation of p62 than control groups, establishing the existence of an autophagic flow. Electron microscopy showed that IPC preserves the structural integrity of mitochondria after ischemia and at the end of reperfusion. Moreover, hearts subjected to IPC exhibited increased mitochondrial ATP synthesis. The beneficial effects of IPC were abolished by W in all trials of this study, abolishing the differences between the IPC and control groups. These results suggest that IPC could partly reduce injury by ischemia-reperfusion (I\/R) by decreasing mitochondrial damage and promoting autophagy. Since W is a nonspecific inhibitor of the PI3Ks family, further research is required to confirm participation of PI3K in the response to IPC.","palabras_clave":"WORTMANNIN|CARDIOPROTECTION|ISCHEMIA|REPERFUSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5734,"idioma_id":4,"titulo":"Thiols of flagellar proteins are essential for progressive motility in human spermatozoa","resumen":"Male infertility is a disorder of the reproductive system defined by the failure to achieve a clinical pregnancyafter 12 months or more of regular unprotected sexual intercourse. The presence of low-motile or immotile spermatozoa isone of many causes of infertility; however, this observation provides little or no information regarding the pathogenesis ofthe malfunction. Good sperm motility depends on correct assembly of the sperm tail in the testis and efficient maturationduring epididymal transit. Thiols of flagellar proteins, such as outer dense fibre protein 1 (ODF1), are oxidised to formdisulfides during epididymal transit and the spermatozoa become motile. This study was designed to determine howoxidative changes in protein thiol status affect progressive motility in human spermatozoa. Monobromobimane (mBBr)was used as a specific thiol marker and disruptor of sperm progressive motility. When mBBr was blocked by dithiothreitolit did not promote motility changes. The analysis of mBBr-treated spermatozoa revealed a reduction of progressivemotility and an increased number of spermatozoa with non-progressive motility without affecting ATP production. Laserconfocal microscopy and western blot analysis showed that one of the mBBr-positive proteins reacted with an antibody toODF1. Monobromobimane fluorescence intensity of the sperm tail was lower in normozoospermic than asthenozoospermicmen, suggesting that thiol oxidation in spermatozoa of asthenozoospermic men is incomplete. Our findingsindicate that mBBr affects the thiol status of ODF1 in human spermatozoa and interferes with progressive motility.","palabras_clave":"Cytoskeleton|Epidydimus|Thiol status|Sperm motility"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5735,"idioma_id":5,"titulo":"Valoración del cambio ambiental según los 'junqueros' y 'leñateros' en el sector sur de la región rioplatense (Argentina)","resumen":"Mediante un abordaje etnoecológico, se realizaron 24 entrevistas a informantes calificados de los partidos de Magdalena y Punta Indio, en el litoral rioplatense, con el objetivo de recopilar y evaluar las narrativas de junqueros y leñateros locales respecto del cambio ambiental y su impacto en sus vidas cotidianas. Estas actividades extractivas (obtención de leña y recolección de juncos) fueron muy importantes en esta zona, aunque en la actualidad sufren una merma, sobre todo en la actividad junquera, debido principalmente a dos acontecimientos que marcaron el rumbo de la actividad: derrame de petróleo y cambios en los sistemas productivos. En la actividad leñera se registraron cambios principalmente como producto de la creación de la Reserva de Biosfera Parque Costero del Sur, sin embargo, a diferencia de los junqueros, la abundancia del recurso leñatero y la reformulación en las prácticas y estrategias de selección hizo posible la permanencia de la actividad a pesar de los cambios sufridos en el área de estudio. Las actividades ejercidas por los informantes influyeron en las narrativas sobre su valoración del cambio ambiental, dado que se reconocieron pocos cambios en común, principalmente, en relación con el clima y la fauna.","palabras_clave":"actividades extractivas|litoral rioplatense|Magdalena|narrativas locales|Punta Indio"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5736,"idioma_id":4,"titulo":"Dopaminergic isoquinolines with hexahydrocyclopenta[ ij ]-isoquinolines as D2-like selective ligands","resumen":"Dopamine receptors (DR) ligands are potential drug candidates for treating neurological disorders including schizophrenia or Parkinson´s disease. Three series of isoquinolines: (E)-1-styryl-1,2,3,4-tetrahydroisoquinolines (series 1), 7-phenyl-1,2,3,7,8,8a-hexahydrocyclopenta[ij]-IQs (HCPIQs) (series 2) and (E)-1-(prop-1-en-1-yl)-1,2,3,4- tetrahydroisoquinolines (series 3), were prepared to determine their affinity for both D1and D2-like DR. The effect of different substituents on the nitrogen atom (methyl or allyl), the dioxygenated function (methoxyl or catechol), the substituent at the &beta;-position of the THIQ skeleton, and the presence or absence of the cyclopentane motif, were studied. We observed that the most active compounds in the three series (2c, 2e, 3a, 3c, 3e, 5c and 5e) possessed a high affinity for D2-like DR and these remarkable features: a catechol group in the IQ-ring and the N-substitution (methyl or allyl). The series showed the following trend to D2-RD affinity: HCPIQs &gt; 1-styryl &gt; 1-propenyl. Therefore, the substituent at the &beta;-position of the THIQ and the cyclopentane ring also modulated this affinity. Among these dopaminergic isoquinolines, HCPIQs stood out for unexpected selectivity to D2-DR since the Ki D1\/D2ratio reached values of 2465, 1010 and 382 for compounds 3a, 3c and 3e, respectively. None of the most active THIQs in D2DR displayed relevant cytotoxicity in human neutrophils and HUVEC. Finally, and in agreement with the experimental data, molecular modeling studies on DRs of the most characteristic ligands of the three series revealed stronger molecular interactions with D2DR than with D1DR, which further supports to the encountered enhanced selectivity to D2DR.","palabras_clave":"Tetrahydroisoquinolines|Hexahydrocyclopentaisoquinolines|Dopaminergic|Structure-activity relationships|Cytotoxicity|Molecular modeling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5737,"idioma_id":4,"titulo":"GABAergic signaling in the rat pineal gland","resumen":"Primera autoría compartida: H. Yu; S.G. Benitez. Ultima autoría compartida: E.M. Muñoz; B. Hille. 25-Mar-2016Dear Prof. Hille:It is a pleasure to accept your manuscript entitled 'GABAergic signaling in the rat pineal gland' in its current form for publication in the Journal of Pineal Research.  The comments of the reviewer(s) who reviewed your manuscript are included at the foot of this letter.Copyright Transfer AgreementYour article cannot be published until the publisher has received the appropriate signed license agreement. Within the next few days the corresponding author will receive an email from Wiley?s Author Services system which will ask them to log in and will present them with the appropriate license for completion.Thank you for your fine contribution.  On behalf of the Editors of the Journal of Pineal Research, we look forward to your continued contributions to the Journal.Sincerely,Dr. Russel ReiterEditor-in-Chief, Journal of Pineal Researchreiter@uthscsa.edu. Abstract: Pinealocytes secrete melatonin at night in response to norepinephrine released from sympathetic nerve terminals in the pineal gland. The gland also contains many other neurotransmitters whose cellular disposition, activity, and relevance to pineal function are not understood. Here we clarify sources and demonstrate cellular actions of the neurotransmitter &#947;-aminobutyric acid (GABA) using Western blotting and immunohistochemistry of the gland and electrical recording from pinealocytes. GABAergic cells and nerve fibers, defined as containing GABA and the synthetic enzyme GAD67, were identified. The cells represent a subset of interstitial cells while the nerve fibers were distinct from the sympathetic innervation. The GABAA receptor subunit &#945;1 was visualized in close proximity of both GABAergic and sympathetic nerve fibers as well as fine extensions among pinealocytes and blood vessels. The GABAB1 receptor subunit was localized in the interstitial compartment but not in pinealocytes. Electrophysiology of isolated pinealocytes revealed that GABA and muscimol elicit strong inward chloride currents sensitive to bicuculline and picrotoxin, clear evidence for functional GABAA receptors on the surface membrane. Applications of elevated potassium solution or the neurotransmitter acetylcholine depolarized the pinealocyte membrane potential enough to open voltage-gated Ca2+ channels leading to intracellular calcium elevations. GABA repolarized the membrane and shut off such calcium rises. In 48-72-h cultured intact glands, GABA application neither triggered melatonin secretion by itself nor affected norepinephrine-induced secretion. Thus strong elements of GABA signaling are present in pineal glands that make large electrical responses in pinealocytes, but physiological roles need to be found.","palabras_clave":"PINEAL GLAND|GABA|GABAA RECEPTOR|MEMBRANE POTENTIAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5738,"idioma_id":4,"titulo":"Analysis of possible genotoxicity of the herbicide flurochloridone and its commercial formulations: Endo III and Fpg alkaline comet assays in Chinese Hamster Ovary (CHO-K1) cells","resumen":"Cytotoxic and genotoxic effects of flurochloridone (FLC) and its formulations Twin Pack Gold®andRainbow®were evaluated in CHO-K1 cells. Using the alkaline single-cell gel electrophoresis (SCGE)assay, we observed that FLC (15 g\/ml), Twin Pack Gold®or Rainbow®induced primary DNA dam-age, increasing the frequency of damaged nucleoids. Vitamin E pretreatment did not modify the effect.Decreased cell viability was observed only in Twin Pack Gold®-treated cultures and was significantlyameliorated by vitamin E. Post-treatment of herbicide-damaged CHO-K1 cells with the enzymes Endo IIIor Fpg did not increase FLC-, Twin Pack Gold®-, or Rainbow®-induced DNA damage. These results demon-strate that neither FLC nor FLC-based formulations induce DNA damage through hydroxyl radical or lipidalkoxyl radical production, and that the induced DNA lesions were not related to oxidative damage atthe purine\/pyrimidine level. Our observations strongly suggest that the cytotoxic effects observed afterTwin Pack Gold®exposure are due to the excipients contained within the technical formulation ratherthan FLC itself.","palabras_clave":"ALKALINE SINGLE GEL ELECTROPHORESIS ASSAY|ALKALINE ENDONUCLEASE-MODIFIED SINGLE-CELL GELL ELECTROPHORESIS|LESION-SPECIFIC ENDONUCLEASES |VITAMIN E"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5739,"idioma_id":4,"titulo":"AN INTEGRATED ON-LINE METHOD FOR THE PRECONCENTRATION AND SIMULTANEOUS DETERMINATION OF METSULFURON METHYL AND CHLORSULFURON USING OXIDIZED CARBON NANOTUBES AND SECOND ORDER FLUORESCENT DATA","resumen":"Trace amounts of two sulfonylurea herbicides widely used for crops protection, metsulfuron methyl (MSM) andchlorsulfuron (CSF) were simultaneously determined taking into account the different kinetic photodegradationbehavior of their photoproducts in alkaline medium. As the analytes are present at trace concentration levels, apreconcentration by sorption on a mini-column packed with oxidized multiwall carbon nanotubes (ox-MWCNTs) at pH 3.0 was performed. The retained analytes were removed from the ox-MWCNTs mini-columnwith a mixture of ACN contained 10% (v\/v) of NaOH pH 12.5. A total enrichment factor of 26-fold for a14.50 mLsample volumewas obtained. The eluate was photodegraded by UV radiation during 126 s and the fluorescentspectra corresponding to the analytes photoproductswere registered overtime between 300 and 500nm.The kinetic second order data were analyzed by unfolded-partial least squares-residual bilinearization (U-PLS\/RBL) and multidimensional-partial least squares-residual bilinearization (N-PLS\/RBL) algorithms. The relativeerror of prediction (REP %) for N-PLS\/RBLwas 7.73% for MSMand 6.37% for CSF. In the case of U-PLS\/RBL, this statisticalparameter was 7.75% for MSM and 7.23% for CSF, respectively. The limits of detection (LOD) were0.19 &#956;g L&#8722;1 for MSM and 1.14 &#956;g L&#8722;1 for CSF using N-PLS\/RBL and 0.21 &#956;g L&#8722;1 for MSM and 1.03 &#956;g L&#8722;1 forCSF when U-PLS\/RBL was applied.The entire procedurewas performed in an on-line integrated fully automated flowsystem coupled to a lowmercuryUV lamp(15 W, 254 nm) and a spectrofluorometer. In this manner, the preconcentration, photodegradationand detection steps were performed in a reproducible way.After optimization, the method was successfully applied to the analysis of real water samples obtained in thesouth part of Buenos Aires province and used for irrigation and consumption.","palabras_clave":"SULFONYLUREAS  |AUTOMATED FLOW SYSTEM|OXIDIZED MULTIWALL CARBON NANOTUBES|PHOTODEGRADATION|SECOND ORDER DATA ALGORITHMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5740,"idioma_id":4,"titulo":"The eoorthid brachiopod Apheoorthina in the Lower Ordovician of NW Argentina and the dispersal pathways along western Gondwana","resumen":"The eoorthid brachiopod Apheoorthina is reported for the first time from the Lower Ordovician of NW Argentina. It is represented by a species similar to A. ferrigena from the Tremadocian of the Prague Basin, increasing the faunal affinities between the Central Andean Basin and the South European microcontinents, in particular the Bohemian region (Perunica). Nine out of the fourteen brachiopod genera reported from the Tremadocian of the Central Andean Basin (~64 %) are shared with the Mediterranean region, four of which (~ 28 %) have been recorded in the Prague Basin, and two (Kvania and Apheoorthina) are restricted to the Central Andes and Perunica. Dispersal pathways around Gondwana are analyzed in the light of major factors affecting large-scale distribution of brachiopods (environment, larval capacity for dispersal, oceanic currents). The presence in Apheoorthina aff. ferrigena of a well-preserved larval protegulum measuring 420 µm in width and 210 µm in length strongly suggests that this species had planktotrophic larvae capable of long-distance dispersal. According to recent ocean-atmosphere general circulation models for the Ordovician Period, the Central Andean margin was dominated by the cold-water Antarctica Current. Despite the complex non-zonal pattern produced by current deflections around the peri-Gondwanan microcontinents, the general westward circulation sense favoured larval dispersal from the Andean region to North Africa, Avalonia, the Armorican Terrane Assemblage, and Perunica. On the other hand, the eastwards flowing Gondwana Current connected the North Gondwana waters with the South American epicontinental seas, which could explain the reversed migration of some brachiopods.","palabras_clave":"BRACHIOPODA|PALAEOBIOGEOGRAPHY|ORDOVICIAN|TREMADOCIAN|GONDWANA|PERUNICA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5741,"idioma_id":4,"titulo":"Homologs of the LapD-LapG c-di-GMP Effector System Control Biofilm Formation by Bordetella bronchiseptica","resumen":"Biofilm formation is important for infection by many pathogens. Bordetella bronchiseptica causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in B. bronchiseptica. In the present work, based on their previously reported function in Pseudomonas fluorescens, we identified three genes in the B. bronchiseptica genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. In vitro and in vivo studies showed that the protease LapG of B. bronchiseptica cleaves the N-terminal domain of BrtA, as well as the LapA protein of P. fluorescens, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a B. bronchiseptica strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA\/LapD\/LapG to modulate biofilm formation, as well as impact pathogenesis, by B. bronchiseptica.","palabras_clave":"Bordetella|c-di-GMP|Biofilm"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5742,"idioma_id":4,"titulo":"Point defects in thorium nitride: A first-principles study","resumen":"Thorium and its compounds (carbides and nitrides) are being investigated as possible materials to be used as nuclear fuels for Generation-IV reactors. As a first step in the research of these materials under irradiation, we study the formation energies and stability of point defects in thorium nitride by means of first-principles calculations within the framework of density functional theory. We focus on vacancies, interstitials, Frenkel pairs and Schottky defects. We found that N and Th vacancies have almost the same formation energy and that the most energetically favorable defects of all studied in this work are N interstitials. These kind of results for ThN, to the best authors' knowledge, have not been obtained previously, neither experimentally, nor theoretically.","palabras_clave":"thorium nitride|point defects|Frenkel pairs|Schottky defects"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5743,"idioma_id":4,"titulo":"Chromosomal Integrity after UV Irradiation Requires FANCD2-Mediated Repair of Double Strand Breaks.","resumen":"Fanconi Anemia (FA) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks (ICLs). FANCD2, a central factor of the FA pathway, is essential for the repair of double strand breaks (DSBs) generated during fork collapse at ICLs. While lesions different from ICLs can also trigger fork collapse, the contribution of FANCD2 to the resolution of replication-coupled DSBs generated independently from ICLs is unknown. Intriguingly, FANCD2 is readily activated after UV irradiation, a DNA-damaging agent that generates predominantly intra-strand crosslinks but not ICLs. Hence, UV irradiation is an ideal tool to explore the contribution of FANCD2 to the DNA damage response triggered by DNA lesions other than ICL repair. Here we show that, in contrast to ICL-causing agents, UV radiation compromises cell survival independently from FANCD2. In agreement, FANCD2 depletion does not increase the amount of DSBs generated during the replication of UV-damaged DNA and is dispensable for UV-induced checkpoint activation. Remarkably however, FANCD2 protects UV-dependent, replication-coupled DSBs from aberrant processing by non-homologous end joining, preventing the accumulation of micronuclei and chromatid aberrations including non-homologous chromatid exchanges. Hence, while dispensable for cell survival, FANCD2 selectively safeguards chromosomal stability after UV-triggered replication stress.","palabras_clave":"FANCD2|UV irradiation|Double Strand Breaks|Homologous Recombination"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5744,"idioma_id":4,"titulo":"Assessing rare earth elements in quartz rich geological samples","resumen":"Sodium peroxide (Na2O2) fusion coupled to Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS\/MS) measurements was used to rapidly screen quartz-rich geological samples for rare earth element (REE) content. The method accuracy was checked with a geological reference material and Instrumental Neutron Activation Analysis (INAA) measurements. The used mass-mode combinations presented accurate results (only exception being 157Gd in He gas mode) with recovery of the geological reference material QLO-1 between 80 to 98% (lower values for Lu, Nd and Sm) and in general comparable to INAA measurements. Low limits of detection for all elements were achieved, generally below 10 pg g-1, as well as measurement repeatability below 15%. Overall, the Na2O2\/ICP-MS\/MS method proved to be a suitable lab-based method to quickly and accurately screen rock samples originating from quartz-rich geological areas for rare earth element content; particularly useful if checking commercial viability.","palabras_clave":"Rare earthelements|ICP-MS|INAA|Quartz"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5745,"idioma_id":4,"titulo":"Elemental contents in the skin and internal tissues of Commerson?s dolphins (Cephalorhynchus commersonii) from Subantarctic waters. Polar Biology","resumen":"The skin of cetaceans is the most accessible tissue, and its sampling has been proposed as a noninvasive method to evaluate trace element concentrations in free-ranging populations. In the present work, concentrations of essential (Cl, Na, K, Mg, Fe, Zn, Mn and Co), nonessential (As and Ag), and of unknown essentiality (Br, Rb and Cs) elements were determined in the skin from nine by-caught Commerson?s dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina. Skin correlations with internal tissues?lung, liver, kidney and muscle?were assessed to evaluate how the skin represents internal element concentration for monitoring purposes. Elemental contents were analyzed by instrumental neutron activation analysis (INAA). Regarding tissue distribution, skin had the highest concentration of Zn being two orders of magnitude higher than internal tissues, while other elements such as Co and Rb had similar concentrations among tissues. High mean concentrations of Cl, Na, Mg, Br and Mn were observed in the lung and liver. Our results support the use of skin to evaluate Fe, Br and Rb concentrations in internal tissues for biomonitoring purposes; however, other elements did not show significant skin-to-tissue correlations. Overall, toxic element levels were far below concentrations found to cause harm in marine vertebrates. This study provided baseline data on elemental concentrations in tissues of Commerson?s dolphins in subantarctic waters from the South Atlantic Ocean.","palabras_clave":"ESSENTIAL ELEMENTS  |TOXIC ELEMENTS |EPIDERMIS|SUBANTARCTIC DOLPHINS|BIOMONITORING|SOUTHWESTERN SOUTH ATLANTIC OCEAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5746,"idioma_id":4,"titulo":"Approaches to unravel pathways of reactive oxygen species in the photoinactivation of bacteria induced by a dicationic fulleropyrrolidinium derivative","resumen":"The photodynamic mechanism sensitized by N,N-dimethyl-2-[4-(3-N,N,N-trimethylammoniopropoxy)phenyl]fulleropyrrolidinium (DPC602+) was investigated in Staphylococcus aureus cells. Different experimental conditions were used to detect reactive oxygen species (ROS) in S. aureus cell suspensions. First, a photoinactivation of 4 log decrease of S. aureus viability was chosen using 0.5 lM DPC60 2+ and 15 minirradiation. An anoxic atmosphere indicated that oxygen was required for an effective photoinactivation. Also, photoprotection was found in the presence of sodium azide, whereas the photocytotoxicity induced by DPC602+ increased in D2O. The addition of diazabicyclo[2.2.2]octane or D-mannitol produced a reduction in the S. aureus photokilling. Moreover, singlet molecular oxygen, O2(1Dg), was detected by the reaction with 9,10-dimethylanthracene into the S. aureus cells. A decrease in the photoinactivation of S. aureus was observed in the presence of b?nicotinamide adenine dinucleotide reduced form, which was dependent on the NADH concentration. Therefore, under aerobic condition the photocytotoxicity activity induced by DPC602+ was mediated by mainly a contribution of type II process. Moreover, photoinactivation of S. aureus was possible with DPC602+ in the presence of azide anions under anoxic condition. However, these conditionswere not effective to photoinactivate Escherichia coli. On the other hand, the addition of potassium iodide produced an increase in the photokilling of bacteria, depending on the KI concentration and irradiation times. The formation of reactive iodine species may be contributing to inactivate S. aureus cells photoinduced by DPC602+.","palabras_clave":"FULLERENE|PHOTODYNAMIC INACTIVATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5747,"idioma_id":4,"titulo":"Chopping and changing: the evolution of the flavin-dependent  monooxygenases","resumen":"Flavin-dependent monooxygenases play a variety of keyphysiological roles and are also very powerful biotechnological tools.These enzymes have been classified into eight different classes (A-H)based on their sequences and biochemical features. By combiningstructural and sequence analysis, as well as phylogenetic inference, wehave explored the evolutionary history of classes A, B, E, F and G, anddemonstrate that their multi-domain architectures reflect theirphylogenetic relationships, suggesting that the main evolutionary stepsin their divergence are likely to have arisen from the recruitment ofdifferent domains. Additionally, the functional divergence within in eachclass appears to have been the result of other mechanisms such as acomplex set of single-point mutations. Our results reinforce the ideathat a main constraint on the evolution of cofactor-dependent enzymes isthe functional binding of the cofactor. Additionally, a remarkablefeature of this family is that the sequence of the key FAD-binding domainis split into at least two parts in all classes studied here, a featurerarely observed in other protein families. We propose a complex set ofevolutionary events that gave rise to the origin of the different classeswithin this family","palabras_clave":"Flavin-dependent monooxygenases|Multi-domain architecture|Cofactor-binding enzymes|Enzyme evolution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5748,"idioma_id":4,"titulo":"Chagas' disease in Aboriginal and Creole communities from the Gran Chaco Region of Argentina: Seroprevalence and molecular parasitological characterization","resumen":"Most indigenous ethnias from Northern Argentina live in rural areas of ?the Gran Chaco? region, where Trypanosoma cruzi is endemic. Serological and parasitological features have been poorly characterized in Aboriginal populations and scarce information exist regarding relevant T. cruzi discrete typing units (DTU) and parasitic loads. This study was focused to characterize T. cruzi infection in Qom, Mocoit, Pit´laxá and Wichi ethnias (N = 604) and Creole communities (N = 257) inhabiting rural villages from two highly endemic provinces of the Argentinean Gran Chaco.DNA extracted using Hexadecyltrimethyl Ammonium Bromide reagent from peripheral blood samples was used for conventional PCR targeted to parasite kinetoplastid DNA (kDNA) and identification of DTUs using nuclear genomic markers. In kDNA-PCR positive samples from three rural Aboriginal communities of ?Monte Impenetrable Chaqueño?, minicircle signatures were characterized by Low stringency single primer-PCR and parasitic loads calculated using Real-Time PCR.Seroprevalence was higher in Aboriginal (47.98%) than in Creole (27.23%) rural communities (Chi square, p = 4.e&#8722; 8). A low seroprevalence (4.3%) was detected in a Qom settlement at the suburbs of Resistencia city (Fisher Exact test, p = 2.e&#8722; 21).The kDNA-PCR positivity was 42.15% in Aboriginal communities and 65.71% in Creole populations (Chi square, p = 5.e&#8722; 4). Among Aboriginal communities kDNA-PCR positivity was heterogeneous (Chi square, p = 1.e&#8722; 4). Highest kDNA-PCR positivity (79%) was detected in the Qom community of Colonia Aborigen and the lowest PCR positivity in two different surveys at the Wichi community of Misión Nueva Pompeya (33.3% in 2010 and 20.8% in 2014).TcV (or TcII\/V\/VI) was predominant in both Aboriginal and Creole communities, in agreement with DTU distribution reported for the region. Besides, two subjects were infected with TcVI, one with TcI and four presented mixed infections of TcV plus TcII\/VI. Most minicircle signatures clustered according to their original localities, but in a few cases, signatures from one locality clustered with signatures from other village, suggesting circulation of the same strains in the area. Parasitic loads ranged from undetectable to around 50 parasite equivalents\/mL, showing higher values than those generally observed in chronic Chagas disease patients living in urban centers of Argentina. Our findings reveal the persistence of high levels of infection in these neglected populations.","palabras_clave":"Chagas disease|Trypanosoma cruzi|discrete typing unit|Aboriginal population|parasitic load|CTAB reagent| polymerase chain reaction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5749,"idioma_id":4,"titulo":"Morphological Changes in Skin Glands During Development in Rhinella arenarum  (Anura: Bufonidae).","resumen":"Avoiding predation is critical to the survival of animals and chemical defenses represent a common strategy among amphibians. In this work, we focused on the morphology of&nbsp; skin glands and types of secretions related to chemical skin defense during ontogeny of&nbsp; Rhinella arenarum by using histological methods. Results showed that prior to metamorphic climax the epidermis contains giant cells typical of bufonids that produce&nbsp; a mucous substance supposedly involved in triggering a flight reaction of the tadpole school. An apical layer of alcianophilic mucus was also observed over the epidermis,&nbsp; which could produce the unpleasant taste of bufonid tadpoles. Giant cells disappear by&nbsp;the onset of the metamorphic climax, when multicellular glands start developing, but the&nbsp; apical layer of mucus remains. By the end of the climax, neither the granular glands of&nbsp; the dorsum nor the parotoid regions are completely developed. Conversely, by the end&nbsp; of metamorphosis the mucous glands are partially developed and produce mucus&nbsp; secretion. Adults show topographic differences in skin secretions: dorsal granular&nbsp; glands located between warts and in the periphery of parotoids possess protein content;&nbsp; granular glands of big warts and in the central region of parotoids contain&nbsp;catecholamines, lipids, and glycoconjugates, whereas ventral granular glands produce&nbsp; acidic glycoconjugates. Mucous glands produce both mucus and proteins. Results&nbsp; suggest that in early juveniles the chemical skin defense mechanisms are not functional. Topographical differences in adult skin secretions suggest that granular glands from the&nbsp; big warts in the skin of R. arenarum produce similar toxins to the parotoid glands.","palabras_clave":"Skin glands|parotoid gland|ontogeny|histology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5750,"idioma_id":4,"titulo":"A comparative ultrastructural analysis of spermatozoa in Pleurodema (Anura, Leptodactylidae, Leiuperinae)","resumen":"This study describes the spermatozoa of 10 of the 15 species of the Neotropical frog genus Pleurodema through transmission electron microscopy. The diversity of oviposition modes coupled with a recent phylogenetic hypothesis of Pleurodema makes it an interesting group for the study of ultrastructural sperm evolution in relation to fertilization environment and egg-clutch structure. We found that Pleurodema has an unusual variability in sperm morphology. The more variable structures were the acrosomal complex, the midpiece, and the tail. The acrosomal complex has allthe structures commonly reported in hyloid frogs but with different degree of development of the subacrosomal cone. Regarding the midpiece, the variability is given by the presence or absence of the mitochondrial collar. Finally, the tail is the most variable structure, ranging from single (only axoneme) to more complex (presence of paraxonemal rod, cytoplasmic sheath, and undulating membrane), with the absence of the typical axial fiber present in hyloid frogs, also shared withsome other genera of Leiuperinae.","palabras_clave":"Ultrastructure|Phylogeny|Foam nest|spermatozoon"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5751,"idioma_id":4,"titulo":"A new species of the Pleistocene furnariid Pseudoseisuropsis (Aves, Passeriformes)","resumen":"Pseudoseisuropsis wintu sp. nov. is described from cranial material that had formerly been designated as a paratypeof the extinct Pseudoseisuropsis nehuen. Thorough comparisons were made between the cranial specimens ascribed toPseudoseisuropsis and many extant furnariid species. This new species is lower Pleistocene in age and increases the known diversityof Pseudoseisuropsis to three species. Cladistic analyses were performed using 43 cranial characters and a backbone constraintbased on a well-supported phylogeny from a recent molecular analysis. In the most parsimonious tree, Pseudoseisuropsis wasretrieved as a sister group of woodcreepers (Dendrocolaptinae) with low support. However, slightly suboptimal hypotheses, whichcould not be completely ruled out, suggest that this genus may be more closely related to other clades of Furnariidae. Hence, weagree with previous authors in regarding Pseudoseisuropsis as Furnariidae Incertae sedis. In addition, two fragmentary fossilsascribed to Pseudoseisuropsis were included in taxonomic comparisons: P. cuelloi, in order to assess the possibility that P. wintu sp.nov. belongs to that species, and another paratype of P. nehuen, to reassess its taxonomic identity.","palabras_clave":"AVES|PASSERIFORMES|PLIOCENO|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5752,"idioma_id":4,"titulo":"Occurrence of mutations in the antimicrobial target genes related to Levofloxacin, Clarithromycin and Amoxicillin resistance in Helicobacter pylori isolates from Buenos Aires City","resumen":"Domain V of 23S rRNA, gyrA and gyrB Quinolones Resistance-Determining Region (QRDR), and pbp-1A gene point mutations were investigated in Helicobacter pylori-resistant isolates from three centres of Buenos Aires. Minimal inhibitory concentrations (MICs) were performed in 197 isolates from 52 H. pylori-positive naive patients by agar dilution method. Point mutations were achieved by amplification and sequencing of the target genes, and their association with resistance was determined by natural transformation assays. Resistance rates were as follows: metronidazole 28.8%, clarithromycin (CLA) 26.9%, levofloxacin (LEV) 32.7%, and amoxicillin (AMX) 7.6%. Nearly one-third of patients carried multidrug-resistant isolates. A2143G or A2142G in domain V of 23S-rRNA was found in all isolates showing high level of resistance to CLA (MIC >2&#8201;mg\/L), accounting for 76.0% (38\/50) of those with the resistant phenotype. The mutations A2267G or T1861C carried by 8\/12 isolates with MIC 1?2&#8201;mg\/L (low level) did not confer resistance by transformation. Substitutions at GyrA position 87 or 91, mainly N87K and D91G, were found in 92.8% (52\/56) of the LEV-resistant isolates: 48 isolates with MIC 4?64&#8201;mg\/L and 4\/8 isolates with MIC 2&#8201;mg\/L. The remaining four harboured K133N, also present in susceptible isolates. None of the substitutions in GyrB demonstrated to confer resistance. Transformation proved that PBP-1A N562Y and\/or T556S substitutions confer the AMX resistance in our isolates, showing an additive effect. In conclusion, the usually reported mutations related to CLA, LEV, and AMX resistance were found in our isolates. However, low-level CLA resistance seems not to be due to mutations in Domain V of 23S rRNA gene.","palabras_clave":"HELICOBACTER|PYLORI|ANTIMICROBIAL|RESISTANCE|MUTATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5753,"idioma_id":4,"titulo":"Structural remodeling during amyloidogenesis of physiological N&#945;-acetylated &#945;-synuclein","resumen":"The misfolding and aggregation of the presynaptic protein &#945;-synuclein (AS) into amyloid fibrils is pathognomonic of Parkinson´s disease, though the mechanism by which this structural conversion occurs is largely unknown. Soluble oligomeric species that accumulate as intermediates in the process of fibril formation are thought to be highly cytotoxic. Recent studies indicate that oligomer-to-fibril AS transition plays a key role in cell toxicity and progression of neurodegeneration. We previously demonstrated that a subgroup of oligomeric AS species are ordered assemblies possessing a well-defined pattern of intermolecular contacts which are arranged into a distinctive antiparallel &#946;-sheet structure, as opposed to the parallel fibrillar fold. Recently, it was demonstrated that the physiological form of AS is N-terminally acetylated (Ac-AS). Here, we first showed that well-characterized conformational ensembles of Ac-AS, namely monomers, oligomers and fibrils, recapitulate many biophysical features of the nonacetylated protein, such as hydrodynamic, tinctorial, structural and membrane-leakage properties. Then, we relied on ATR-FTIR spectroscopy to explore the structural reorganization during Ac-AS fibrillogenesis. We found that antiparallel &#946;-sheet transient intermediates are built-up at early stages of aggregation, which then evolve to parallel &#946;-sheet fibrils through helix-rich\/disordered species. The results are discussed in terms of regions of the protein that might participate in this structural rearrangement. Our work provides new insights into the complex conformational reorganization occurring during Ac-AS amyloid formation.","palabras_clave":"SYNUCLEIN|AMILOIDOGENESIS|PARKISON DISEASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5754,"idioma_id":4,"titulo":"Chirality in a quaternionic representation of the  genetic code","resumen":"A quaternionic representation of the genetic code, previously reported by the authors (BioSystems 141(10?19), 2016), is updated in order to incorporate chirality of nucleotide bases and amino acids. Theoriginal representation associates with each nucleotide base a prime integer quaternion of norm 7 andinvolves a function that assigns to each codon, represented by three of these quaternions, another inte-ger quaternion (amino acid type quaternion). The assignation is such that the essentials of the standardgenetic code (particularly its degeneration) are preserved. To show the advantages of such a quater-nionic representation we have designed an algorithm to go from the primary to the tertiary structureof the protein. The algorithm uses, besides of the type quaternions, a second kind of quaternions withreal components that we additionally associate with the amino acids according to their order along theproteins (order quaternions). In this context, we incorporate chirality in our representation by observingthat the set of eight integer quaternions of norm 7 can be partitioned into a pair of subsets of cardinalityfour each with their elements mutually conjugate and by putting them into correspondence one to onewith the two sets of enantiomers (D and L) of the four nucleotide bases adenine, cytosine, guanine anduracil, respectively. We then propose two diagrams in order to describe the hypothetical evolution ofthe genetic codes corresponding to both of the chiral systems of affinities: D-nucleotide bases\/L-aminoacids and L-nucleotide bases\/D-amino acids at reading frames 5&#8594; 3and 3&#8594; 5, respectively. Guided bythese diagrams we define functions that in each case assign to the triplets of D- (L-) bases a L- (D-) aminoacid type integer quaternion. Specifically, the integer quaternion associated with a given D-amino acidis the conjugate of that one corresponding to the enantiomer L. The chiral type quaternions obtained forthe amino acids are used, together with a common set of order quaternions, to describe the folding of thetwo classes, L and D, of homochiral proteins.","palabras_clave":"GENETIC CODE REPRESENTATION|HOMOCHIRALITY|HOMOCHIRAL PROTEIN FOLDING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5755,"idioma_id":4,"titulo":"An updated description of Galianthe vaginata (Spermacoceae, Rubiaceae), a species endemic to the Serra da Mantiqueira and Serra do Mar, Southeast Brazil","resumen":"An updated description of Galianthe vaginata is here presented. This species is endemic to the Serra da Mantiqueira and Serra do Mar, Southeast Brazil, and occurs in remnants of the Atlantic forest phytogeographic domain, in the campos de altitude, rocky outcrops, and along trails in montane rainforest. Its bracts, short-styled flowers, fruits and seeds are described and illustrated for the first time. The conspicuously winged stems and the pubescent stipules constitute a combination of diagnostic characters useful for distinguishing G. vaginata from G. polygonoides. Its conservation status is reassessed based on recent collections.","palabras_clave":"amendment|Heterostyly|Spermacoce Clade|Conservation status"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5756,"idioma_id":4,"titulo":"Abundance of the sponge Hymeniacidon cf. perlevis in a stressful environment of Patagonia: relationships with Ulva lactuca and physical variables","resumen":"Understanding the variables (biotic or abiotic) controlling coastal communities is the main goal of ecology research and it is crucial to predict how communities will evolve under the increasing pressure on coastal systems by human activities. The general aims of this study were to assess the variables affecting the distribution of the fast-growing sponge Hymeniacidon cf. perlevis, and to evaluate if physical stressful conditions have an influence in the relationship between Hymeniacidon and the sea lettuce Ulva lactuca. Specifically, the aims were to evaluate the abundance patterns of Hymeniacidon and Ulva and to assess the relationship of Ulva and physical variables on the abundance of Hymeniacidon at both intertidal and subtidal. Hymeniacidon and Ulva showed opposite abundance patterns. Hymeniacidon was more abundant in sites without Ulva, and negatively correlated with Ulva abundance at both heights. Intertidal Hymeniacidon abundance was not correlated with physical variables, but subtidal Hymeniacidon was.","palabras_clave":"sponges|macroalgae|negative associations|physical variables|distribution pattern|intertidal-subtidal heights"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5757,"idioma_id":4,"titulo":"Molecular epidemiology of hepatitis B virus in Misiones, Argentina","resumen":"Hepatitis B virus (HBV) infection is a major public health problem worldwide. The aims of this study were to describe the molecular epidemiology of HBV in the province of Misiones, Argentina and estimate the phylodynamic of the main groups in a Bayesian coalescent framework. To this end, partial or complete genome sequences were obtained from 52 blood donor candidates.The phylogenetic analysis based on partial sequences of S\/P region showed a predominance of genotype D (65.4%), followed by genotype F (30.8%) and genotype A as a minority (3.8%). At subgenotype level, the circulation of subgenotypesD3 (42.3%), D2 (13.5%), F1b (11.5%) and F4 (9.6%) was mainly identified.The Bayesian coalescent analysis of 29 complete genome sequences for the main groups revealed that the subgenotypes D2 and D3 had several introductions to the region, with ancestors dating back from 1921 to 1969 and diversification events until the late ´70s. The genotype F in Misiones has a more recent history; subgenotype F4 isolates were intermixed with sequences from Argentina and neighboring countries and only one significant cluster dated back in 1994 was observed. Subgenotype F1b isolates exhibited low genetic distance and formed a closely related monophyletic cluster, suggesting a very recent introduction. In conclusion, the phylogenetic and coalescent analyses showed that the European genotype D has a higher circulation, a longer history of diversification and may be responsible for the largest proportion of chronic HBV infections in the Province of Misiones. Genotype F, especially subgenotype F1b, had a more recent introduction and its diversification in the last 20 years might be related to its involvement in new transmission events.","palabras_clave":"HEPATITIS B VIRUS|MOLECULAR EPIDEMIOLOGY|ARGENTINA|PHYLOGENETIC ANALYSIS|BAYESIAN COALESCENT ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5758,"idioma_id":4,"titulo":"Draft genome sequence of Bacillus subtilis ALBA01, a strain with antagonistic activity against the soil-borne fungal pathogen of onion Setophoma terrestris","resumen":"Bacillus subtilis is a nonpathogenic bacterium that lives in soil and has long been used as biological control agent in agriculture. Here, we report the genome sequence of a B. subtilis strain isolated from rhizosphere of onion that shows strong biological activity against the soilborne fungal pathogen Setophoma terrestris.","palabras_clave":"BACILLUS SUBTILIS |SETOPHOMA TERRESTRIS|ANTIGUNGAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5759,"idioma_id":5,"titulo":"NUEVOS DATOS PARA LAS INHUMACIONES EN URNAS DE SITIOS ARQUEOLÓGICOS GUARANÍES DEL EXTREMO MERIDIONAL DE LA CUENCA DEL PLATA","resumen":"Entre los complejos comportamientos mortuorios que se observan en los sitios arqueológicospertenecientes a la unidad arqueológica Guaraní, se registran las inhumaciones en urnas. Losestudios vinculados con el análisis de estas estructuras mortuorias tienen escasos antecedentesen la bibliografía académica en general, y particularmente para el extremo meridional de la cuencadel Plata. El objetivo de este trabajo, precisamente, es contribuir al mismo mediante el análisisde los elementos óseos incluidos en urnas mortuorias recuperadas en sitios guaraníes del Deltadel Paraná (Argentina). Esta región constituye el punto más meridional de la expansión de estosgrupos de origen amazónico, cuyo registro mortuorio posee un buen estado de conservación, adiferencia de lo que sucede en otras regiones donde se distribuye esta unidad arqueológica. Eneste trabajo analizamos los restos óseos recuperados en urnas funerarias de los sitios ArroyoMalo, Arroyo Fredes y Arroyo La Glorieta (Delta del Paraná), que fueron excavados a principiosdel siglo XX por diversos investigadores y que hoy forman parte de las colecciones museísticas delMuseo de La Plata. Se estudió la composición sexual, etaria y anatómica de los conjuntos óseos,como así también una serie de variables tafonómicas. Los resultados muestran la preponderanciade individuos masculinos adultos, representados básicamente por huesos largos y elementos delcráneo, algunos de ellos con huellas de corte y pintura roja (ocre) en cantidades abundantes. Estosindicadores, junto a otros de origen tafonómico, señalan que las inhumaciones son secundarias,producto de la desarticulación, descarne y selección de determinadas unidades anatómicas.","palabras_clave":"GUARANIES|ENTIERROS EN URNAS|HUELLAS DE CORTE|DELTA DEL PARANA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5760,"idioma_id":4,"titulo":"Physicochemical, interfacial and emulsifying properties of a non-conventional exudate gum (Prosopis alba) in comparison with gum arabic","resumen":"This work investigated the physicochemical and emulsifying properties of a novel exudate gum obtained from Prosopis alba, a widely spread tree from arid and semiarid regions of South America, in comparison with the well-known arabic gum (GA). Compositional analysis, intrinsic viscosity and structural characterization by FT-IR as well as gums emulsifying properties, evaluated in terms of mean droplet size distribution of O\/W emulsions, &#950;-potential, polidispersity and creaming indexes, were performed and related to dynamic interfacial tension and rheological behavior of the films formed at oil\/water interface. In general, gums displayed a similar composition with carbohydrates representing the higher fraction in both gums (62 and 65 % d.b. for G and GA, respectively). The major difference was observed in the protein content which was higher in Prosopis alba gum and largely explains its better interfacial properties. FTIR analysis further supported that the gums share essentially a similar chemical nature. Emulsifying ability, polidispersity and creaming index during storage, showed that G is able to stabilized emulsions better than GA. Furthermore, the formation of stronger viscoelastic films at the oil\/water interface as well as the charge distribution of the adsorbed G fractions could contribute to understand the higher stability of G emulsions. The evidenced interfacial and emulsifying properties of G support its potential application in emulsion-based foods. Present results could contribute to consider Prosopis alba exudate gum as a novel non-conventional gum with similar or even better emulsifying properties than GA.","palabras_clave":"PROSOPIS ALBA|EXUDATE GUM|INTERFACIAL ACTIVITY|EMULSIFYING PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5761,"idioma_id":4,"titulo":"Diverse Bacterial Groups Contribute to the Alkane Degradation Potential of Chronically Polluted Subantarctic Coastal Sediments","resumen":"We aimed to gain insight into the alkane degradation potential of microbial communities from chronically-polluted sediments of a subantarctic coastal environment using a combination of metagenomic approaches. A total of 6,178 sequences annotated as alkane-1-monooxygenases (EC 1.14.15.3) were retrieved from a shotgun metagenomic dataset that included two sites analyzed in triplicate. The majority of the sequences binned with AlkB described in Bacteroidetes (32 ± 13%) or Proteobacteria (29 ± 7%), although a large proportion remained unclassified at the phylum level. Operational taxonomic unit (OTU)-based analyses showed small differences in AlkB distribution among samples that could be correlated with alkane concentrations, as well as with site-specific variations in pH and salinity. A number of low-abundance OTUs, mostly affiliated with Actinobacterial sequences, were found to be only present in the most contaminated samples. On the other hand, the molecular screening of a large-insert metagenomic library of intertidal sediments from one of the sampling sites identified two genomic fragments containing novel alkB gene sequences, as well as various contiguous genes related to lipid metabolism. Both genomic fragments were affiliated with the phylum Planctomycetes, and one could be further assigned to the genus Rhodopirellula due to the presence of a partial sequence of the 23S rRNA gene. This work highlights the diversity of bacterial groups contributing to the alkane degradation potential and reveals patterns of functional diversity in relation with environmental stressors in a chronically polluted, high-latitude coastal environment. In addition, alkane biodegradation genes are described for the first time in members of Planctomycetes","palabras_clave":"ALKANE MONOOXYGENASE|COASTAL SEDIMENTS|METAGENOMICS|HYDROCARBON POLLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5762,"idioma_id":4,"titulo":"Metagenomics unveils the attributes of the alginolytic guilds of sediments from four distant cold coastal environments","resumen":"Alginates are abundant polysaccharidesin brown algae that constitute an important energy source for marineheterotrophic bacteria. Despite the key role of alginate degradation processesin the marine carbon cycle, little information is available on the bacterialpopulations involved in these processes. The aim of this work was to gain abetter understanding of alginate utilization capabilities in cold coastalenvironments. Sediment metagenomes from four highlatitude regions of bothHemispheres were interrogated for alginate lyase gene homolog sequences andtheir genomic context. Sediments contained highly abundant and diversebacterial assemblages with alginolytic potential, including members ofBacteroidetes and Proteobacteria, as well as several poorly characterized taxa.The microbial communities in Arctic and Antarctic sediments exhibited the mostsimilar alginolytic profiles, whereas brackish sediments showed distinctstructures with a higher proportion of novel genes. Examination of the gene neighborhoodof the alginate lyase homologs revealed distinct patterns depending on the potentiallineage of the scaffolds, with evidence of evolutionary relationships amongalginolytic gene clusters from Bacteroidetes and Proteobacteria. Thisinformation is relevant for understanding carbon fluxes in cold coastalenvironments and provides valuable information for the development ofbiotechnological applications from brown algae biomass.","palabras_clave":"ALGINATE LYASE|METAGENOME|COLD SEDIMENTS|ANTARCTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5763,"idioma_id":4,"titulo":"Revision of Plumarius Philippi 1873 (Hymenoptera: Chrysidoidea, Plumariidae) from Argentina north of Patagonia, with description of eighteen new species","resumen":"Twenty six species of Plumarius from Argentina north of Patagonia are revised. Eighteen new species: Plumarius politus sp. nov., P. sarophorus sp. nov., P. globulosus sp. nov., P. angulatus sp. nov., P. noa sp. nov., P. divergens sp. nov., P. malaris sp. nov., P. gracilior sp. nov., P. riojanus sp. nov., P. exophthalmus sp. nov., P. planatus sp. nov., P. albinervis sp. nov., P. truncatus sp. nov., P. mikrommatus sp. nov., P. obscurus sp. nov., P. gracilis sp. nov., P. fidalgoi sp. nov., and P. ocellulatus sp. nov. are described and illustrated. A new record for known species as well as a key to 26 known species in the region is given. A redescription of P. tumidulus Papp, 2000, which is the first record of the family Plumariidae from Uruguay, is presented.","palabras_clave":"PLUMARIUS|SYSTEMATICS|NEOTROPICAL REGION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5764,"idioma_id":4,"titulo":"Bioethanol production by reusable Saccharomyces cerevisiae immobilized in a macroporous monolithic hydrogel matrices.? Lucinda Mulko, Claudia R. Rivarola*, Cesar A. Barbero, Diego F. Acevedo*.","resumen":"Performance of yeasts on industrial processes can be dramatically improved by immobilization of the biocatalyst. The immobilization of Saccharomyces cerevisiae inside monolithic macroporous hydrogels were produced by in-situ polymerization of acrylamide around a live yeast suspension under cryogelation conditions. Preculture of the yeasts was not necessary and this innovative and simple procedure is amenable to scaling-up to industrial production. The yeasts were efficiently retained in monolithic hydrogels, presenting excellent mechanical properties and high cell viability. Macroporous hydrogels showed a fast mass transport allowing the hydrogel-yeast complexes achieved similar ethanol yield and productivity than free yeasts, which is larger than those reached with yeasts immobilized in compact hydrogels. Moreover, the same yeasts were able to maintain its activity by up to five reaction cycles with a cell single batch during fermentation reactions.","palabras_clave":"BIOETHANOL PRODUCTION|HETEROGENEOUS CATALYSTS|REUSABLE CATALYST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5765,"idioma_id":4,"titulo":"A Bacterial Glycoengineered Antigen for Improved Serodiagnosis of Porcine Brucellosis","resumen":"Brucellosis is a highly zoonotic disease that affects animals and human beings. Brucella suis is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. Laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti O-polysaccharide antibodies are the most sensitive tests. Here, we validate a recombinant glycoprotein antigen, an N-formylperosamine O-polysaccharide-protein conjugate (OAg-AcrA), for diagnosis of porcine brucellosis. An indirect immunoassay based on the detection of anti-O-polysaccharide IgG antibodies was developed coupling OAg-AcrA to enzyme-linked immunosorbent assay plates (glyco-iELISA). To validate the assay, 563 serum samples obtained from experimentally infected and immunized pigs, as well as animals naturally infected with B. suis biovar 1 or 2, were tested. A receiver operating characteristic (ROC) analysis was performed, and based on this analysis, the optimum cutoff value was 0.56 (relative reactivity), which resulted in a diagnostic sensitivity and specificity of 100% and 99.7%, respectively. A cutoff value of 0.78 resulted in a test sensitivity of 98.4% and a test specificity of 100%. Overall, our results demonstrate that the glyco-iELISA is highly accurate for diagnosis of porcine brucellosis, improving the diagnostic performance of current serological tests. The recombinant glycoprotein OAg-AcrA can be produced in large homogeneous batches in a standardized way, making it an ideal candidate for further validation as a universal antigen for diagnosis of 'smooth' brucellosis in animals and humans.","palabras_clave":"recombinant glycoproteins|ELISA|diagnosis|swine brucellosis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5766,"idioma_id":4,"titulo":"DFT Study of Hydrogen-Assisted Dissociation of CO by HCO, COH, and HCOH Formation on Fe(100)","resumen":"DFT calculations using the GGA-PBE exchange correlation functional were used to investigate the effect of the hydrogen in the assisted CO dissociation on the Fe(100) surface. The formation mechanisms of the primary products CH, OH, CH2, and H2O involved in the Fischer?Tropsch synthesis have been studied. Three different routes were investigated passing through the HCO, COH, or HCOH intermediates. The energy barriers of the reactions were estimated using the nudged elastic band method (NEB). The energy profiles of assisted and double-assisted dissociations of CO are presented. The formation energies of HCO, COH, and HCOH intermediates are estimated to be endothermic with activation energies of 0.90, 1.07, and 2.13 eV, respectively. The formation of CH2 is energetically more favorable with the global reaction energy estimated to be &#8722;1.10 eV. The other CH, OH, and H2O intermediates have also endothermic formation energies with respect to the Fe(100)\/(CO + H) system. The chemical bonding of the adsorbed intermediates and reactants was analyzed based on the population analysis, electron localization function, and pseudodifferential charge density. A comparison with direct CO dissociation leads to the conclusion that hydrogen-assisted processes constitute viable routes for CO dissociation on Fe (100) and alkanes formation.","palabras_clave":"Density Functional Theory|Chemical Reaction|Adsorption|Fischer-Tropsch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5767,"idioma_id":5,"titulo":"Letras retenidas. Experiencias de internamiento en las cartas de los pacientes del Manicomio de Santa Isabel de Leganés, Madrid (1900 ? 1950)","resumen":": Las manifestaciones escritas de los sujetos internados en una institución mental han sidoutilizadas con alguna frecuencia para valorar y analizar la subjetividad del paciente. Este trabajopretende estudiar ciertas escrituras de pacientes mentales como manifestaciones de una culturaescrita desarrollada en el espacio cerrado del manicomio y en las que pueden identificarse desdecartas de súplica hasta expresiones de resistencia. Las fuentes utilizadas provienen del ArchivoClínico del antiguo Manicomio Nacional de Santa Isabel en Leganés (España), donde hemoslocalizado una significativa colección de cartas que nunca llegaron a su destino. Unas cartas cuyoscontenidos muestran el funcionamiento y la vida cotidiana del establecimiento desde la perspectivadel paciente, así como sus sentimientos, emociones y experiencias.","palabras_clave":"Cartas|Manicomio|Leganes|Subjetividad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5768,"idioma_id":4,"titulo":"A substituted sulfonamide and its Co (II), Cu (II) and Zn (II) complexes as potential antifungal agents","resumen":"A sulfonamide 1-tosyl-1-H-benzo(d)imidazol-2-amine (TBZA) and three new complexes of Co(II), Cu(II), and Zn(II) have been synthesized. The compounds have been characterized by elemental analyses, FTIR, 1H, and 13C-NMR spectroscopy. The structure of the TBZA, and its Co(II) and Cu(II) complexes, was determined by X-ray diffraction methods. TBZA and its Co(II) complex crystallize in the triclinic P-1 space group, while the Cu(II) complex crystallizes in the monoclinic P21\/c space group. Antifungal activity was screened against eight pathogenic yeasts: Candida albicans (DMic 972576), Candida krusei (DMic 951705), Candida glabrata (DMic 982882), Candida tropicalis (DMic 982884), Candida dubliniensis (DMic 93695), Candida guilliermondii (DMic 021150), Cryptococcus neoformans (ATCC 24067), and Cryptococcus gattii (ATCC MYA-4561). Results on the inhibition of various human (h) CAs, hCA I, II, IV, VII, IX, and XII, and pathogenic beta and gamma CAs are also reported.","palabras_clave":"SUBSTITUTED SULFONAMIDE|COMPLEXES|ANTIFUNGAL AGENTS|PATHOGENIC YEASTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5769,"idioma_id":4,"titulo":"Integrated extraction and purification of soy isoflavones by using aqueous micellar systems","resumen":"In this work, an integration of solid-liquid and liquid-liquid extractions by using aqueous micellar two-phase systems was evaluated as potential tool to purify soy isoflavones. Additionally, the proposed methodology aimed to preserve the protein content of the processed soy flour. The extractive assays were performed in AMTPS formed by Triton X-114 and sodium tartrate. In order to optimize the purification process, temperature and time were evaluated as independent variables. Under optimal working conditions, i.e. 100 min and 33 °C of incubation, IF were purified with a recovery percentage of 93 and a purification factor of almost 10. More importantly, the obtained sample presented an aglycone proportion superior to the reported by other methodologies. These results open perspectives to the use of aqueous micellar two-phase systems as an integrative methodology to extract, concentrate and purify isoflavones.","palabras_clave":"SOYBEAN FLOUR|ISOFLAVONE|PURIFICATION|AQUEOUS MICELLAR TWO-PHASE SYSTEM|SURFACTANT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5770,"idioma_id":5,"titulo":"PROGRAMA ICONOGRÁFICO Y MATERIAL EN LAS PINTURAS MURALES DE LA IGLESIA DE SAN ANDRES DE PACHAMA, CHILE","resumen":"Laiglesia de San Andrés de Pachama conserva un programa de pintura muralejecutado durante la segunda mitad del siglo XVIII en la localidadprecordillerana del mismo nombre. El objetivo del presente trabajo es caracterizarel repertorio de imágenes contenidos en los muros del templo, proponer sufuncionalidad en el contexto de la catequesis, a la vez que introducir análisisiniciales de su dimensión material. ","palabras_clave":"PINTURA MURAL|PIGMENTOS|AGLUTINANTES|IDOLATRIA|CATEQUESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5771,"idioma_id":4,"titulo":"Polyhydroxylated sulfated steroids derived from 5a-cholestanes as antiviral agents against herpes simplex virus","resumen":"Twelve polyhydroxylated sulfated steroidssynthesized from a 5a-cholestane skeleton with differentsubstitutions in C-2, C-3 and C-6 were evaluated forcytotoxicity and antiviral activity against herpes simplexvirus (HSV) by a virus plaque reduction assay. Fourcompounds elicited a selective inhibitory effect againstHSV. The disodium salt of 2b,3a-dihydroxy-6E-hydroximine-5a-cholestane-2,3-disulfate, named compound 7,was the most effective inhibitor of HSV-1, HSV-2 andpseudorabies virus (PrV) strains, including acyclovir-resistantvariants, in human and monkey cell lines. Preliminarymechanistic studies demonstrated that compound 7did not affect the initial steps of virus entry but inhibited asubsequent event in the infection process of HSV.","palabras_clave":"HERPES SIMPLEX VIRUS|ANTIVIRAL ACTIVITY|POLYHYDROXYLATED SULFATED STEROIDS|5-A-CHOLESTANES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5773,"idioma_id":4,"titulo":"The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity","resumen":"Host responses against metazoan parasites or an array of environmental substances elicit type 2 immunity. Despite its protective function, type 2 immunity also drives allergic diseases. The mechanisms that regulate the magnitude of the type 2 response remain largely unknown. Here, we show that genetic ablation of a receptor tyrosine kinase encoded byTyro3in mice or the functional neutralization of its ortholog in human dendritic cells resulted in enhanced type 2 immunity. Furthermore, the TYRO3 agonist PROS1 was induced in T cells by the quintessential type 2 cytokine, interleukin-4. T cell-specificPros1knockouts phenocopied the loss ofTyro3 Thus, a PROS1-mediated feedback from adaptive immunity engages a rheostat, TYRO3, on innate immune cells to limit the intensity of type 2 responses. ","palabras_clave":"Tyro3|Th2|Allergy\/Parasite infection|Protein S"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5774,"idioma_id":4,"titulo":"KatG, the Bifunctional Catalase of Xanthomonas citri subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves","resumen":"Xanthomonas citri subsp. citri(Xcc) is the bacterium responsible for citrus canker. This bacterium is exposedto reactive oxygen species (ROS) at different points during its life cycle,including those normally produced by aerobic respiration or upon exposition toultraviolet (UV) radiation. Moreover, ROS are key components of the host immuneresponse. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H2O2,avoiding the potential damage caused by this specie. Xcc genome includes fourcatalase genes. In this work, we studied the physiological role of KatG, theonly bifunctional catalase of Xcc, through the construction andcharacterization of a modified strain (XcckatG), carrying an insertional mutation in the katG gene. First, we evaluated theinvolvement of KatG in the bacterial adaptive response to H2O2.XcckatGcultures exhibited lower catalase activity than those of the wild-type strain,and this activity was not induced upon treatment with sub-lethal doses of H2O2. Moreover, the KatG-deficientmutant exhibited decreased tolerance to H2O2 toxicity comparedto wild-type cells and accumulated highintracellular levels of peroxides upon exposure to sub-lethal concentrations of H2O2. To further study the role of KatG in Xcc physiology,we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. Inboth conditions, XcckatG showed ahigh mortality in comparison to Xcc wild-type. Finally, we studied thedevelopment of bacterial biofilms. While structured biofilms were observed forthe Xcc wild-type, the development of these structures was impaired for XcckatG. Based on these results, we demonstrated that KatG is responsible for Xcc adaptiveresponse to H2O2 and a key component of the bacterialresponse to oxidative stress. Moreover, this enzyme plays an important roleduring Xcc epiphytic survival, being essential for biofilm formation and UVresistance.","palabras_clave":"XANTHOMONAS CITRI SUBSP. CITRI|KATG CATALASE|HYDROGEN PEROXIDE RESPONSE|EPIPHYTIC SURVIVAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5775,"idioma_id":4,"titulo":"Forged Under the Sun: Life and Art of Extremophiles from Andean Lakes","resumen":"High Altitude Andean Lakes (HAAL) are a treasure chest for microbiological research inSouth-America. Their indigenous microbial communities are exposed to extremely high UVirradiation and to multiple chemical extremes (Arsenic, high salt content, alkalinity). Microbesare found both, free-living or associated into microbial mats with different degrees ofmineralization and lithification, including unique modern stromatolites located at 3,570 m abovesea level. Characterization of these poly-extremophilic microbes began only recently, employingmorphological and phylogenetic methods as well as high throughput sequencing and proteomicsapproach. Apart from providing a general overview on microbial communities, special attentionis given to various survival strategies; HAAL?s microbes present a complex system of sharedgenetic and physiological mechanisms (UV-resistome) based on UV-photoreceptors and stresssensors with their corresponding response-regulators, UV avoidance and protection strategies,damage tolerance, and UV-damage repair. Molecular information will be provided for what is,so far the most studied HAAL molecule, a CPD-Class I photolyase from Acinetobacter Ver3(Laguna Verde, 4,400 m). This work further proposes some strategies that make an appeal forthe preservation of HAAL highly, fragile environment that offers promising and ample researchpossibilities.","palabras_clave":"photoreceptors|photobiology|lakes|Puna"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5776,"idioma_id":4,"titulo":"Functional Green-Tuned Proteorhodopsin from Modern Stromatolites","resumen":"The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (&#964;1 =3.5&#956;s) into another red-shifted intermediate. This species decayed in a two-step process (&#964;2 =84&#956;s, &#964;3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.","palabras_clave":"Exiguobacterium|stromatolites|Laguna Socompa|irradiation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5777,"idioma_id":4,"titulo":"Wetland loss due to land use change in the Lower Paraná River Delta, Argentina","resumen":"Wetland loss is a process of global concern because wetlands are highly diverse ecosystems that provide important goods and services. Land use and land cover changes in wetlands thus threaten both biodiversity and human well-being. The Paraná River Delta is one of the largest and most important wetland ecosystems of South America. However, cattle and forestry activities are both expanding and intensifying rapidly, accompanied by widespread water control practices (e.g., dams and ditches), with unknown consequences for wetland landscape structure and function. To understand the patterns and drivers of land cover change in the Lower Paraná River Delta, we quantified land cover changes and modeled associated factors. We classified Landsat images from 1999 and 2013 to generate land cover maps for the Lower Delta and identified areas of land cover changes, especially the conversion of freshwater marshes into pastures. We quantified the influence of different socioeconomic (distance to roads, population centers and human activities centers), land management (area behind dams, cattle density and years since last fire),biophysical variables (landscape unit, elevation, soil productivity, distance to rivers) and variables related to extreme system dynamics (flooding and fires)on freshwater marsh conversion with Boosted Regression Trees. We found that one third of the freshwater marshes of the Lower Delta were lost in only 14 years (163,000 ha). The majority (70%) of the losses were due to conversion to pastures and 18% due to conversion to forestry plantations. Cattle density, dammed area and accessibility were the most important factors for freshwater marsh conversion to pastures. These rapid and widespread losses of freshwater marshes have potentially large negative consequences for biodiversity and ecosystem services and the lack of a land use policy to mitigate these losses is of concern. A strategy for sustainable wetland management will benefit from careful analysis of dominant land uses and the related management practices, taking into account the characteristics of the landscape.","palabras_clave":"WETLAND CONVERSION|FRESHWATER MARSHES|CATTLE GRAZING|FORESTRY|POLDERS|REMOTE SENSING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5778,"idioma_id":4,"titulo":"Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi","resumen":"The present study aimed to investigate the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was carried out using culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their activity of nitrate reductase. The role of the mentioned enzyme in the production of nanoparticles was assessed by using inhibitors. The characterization of produced Ag NPs was made by UV-visible spectrophotometry, photon correlation spectroscopy, transmission electron microscopy and X-ray diffraction. Moreover, the stabilizing agents that surround the Ag NPs were identified by FTIR spectrum. The Ag NPs obtained from both yeasts were disperse and stable and showed differences in sizes, zeta potential, concentration and surrounding compounds. The antifungal activity of Ag NPs was evaluated against relevant phytopathogenic fungi, commonly producers of postharvest diseases. Tests ?in vitro? showed that, the antifungal activity of nanoparticles from R. glutinis was higher than the ones from C. laurentii and both were significantly more effectives for inhibiting the fungi that the nanoparticles of chemical synthesis. At one concentration of 3 ppm, nanoparticles from R. glutinis were similarly effectives than iprodione, a fungicide commonly utilized for combating postharvest diseases.","palabras_clave":"Cryptococcus laurentii|Rhodotorula glutinis|Silver nanoparticles|Antifungal activity|Postharvest phytopathogen fungi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5779,"idioma_id":4,"titulo":"Beautiful mirrors for a pNGB Higgs","resumen":"We consider one of the most significant deviations from the Standard Model: the forward-backward asymmetry of the b-quark measured at leptonic colliders. We investigate the possibility to solve this discrepancy by introducing new physics at the TeV scale. We focus on models where the Higgs is a pseudo Nambu-Goldstone boson of a new strongly coupled sector with a global SO(5) symmetry broken spontaneously to SO(4). Besides the usual top partners, we introduce bottom partners in the representations 16 and 4 of SO(5) and show that they can improve significantly the fit by correcting the Zbb couplings. We also estimate the corrections to the couplings at one-loop and obtain that the tree-level ones dominate and can give a reliable estimation. We find that the large shift required for Zb_Rb_R leads to light custodians associated to the b-quark, similar to the top partners, as well as a rich phenomenology involving neutral interactions in the bottom-sector.","palabras_clave":"HIGGS MODEL: COMPOSITE|SYMMETRY: SO(5)|SO(5)\/SO(4)|ANGULAR DISTRIBUTION: ASYMMETRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5780,"idioma_id":5,"titulo":"Subalternidad, pos-decolonialidad y cultura popular: nuevas navegaciones en tiempos nacional-populares","resumen":"¿Qué vínculo existe entre subalternidad, estudios poscoloniales y opcióndecolonial? ¿En qué medida estas perspectivas pueden conducirnos a reflexionar entorno a la cultura popular? ¿Cómo impactan las recientes experiencias nacional-popularesen Latinoamérica en nuestros estudios, así como sus retrocesos y crisis? ¿En quémedida es posible ?y sensato? seguir hablando de ?lo popular? hoy? Para volver sobreestas interrogantes, que planteamos por primera vez hace casi diez años, revisar nuestrashipótesis y volver a afirmar ciertas constantes es que proponemos una reflexiónen torno a lo popular a partir de tres dimensiones de la subalternidad: la del conflictoy sus derivas; las aporías de sus metáforas; la inflexión de la colonialidad. Buscamosvolver a afirmar lo popular como categoría de análisis que reivindica lo político, en sucruce con perspectivas contemporáneas innovadoras","palabras_clave":"CULTURA POPULAR|SUBALTERNIDAD|COLONIALIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5781,"idioma_id":4,"titulo":"Coinfection of Leishmania (Viannia) braziliensis and Streptococcus pneumoniae in multiple cutaneous lesions","resumen":"A 12-year-old girl was seen by a physician in Frias (Santiago del Estero Province, northernArgentina region) due to a lesion in her left leg that presented a painless papule, which laterbecame ulcerative. The mother referred that the first lesion appeared after an insect bite in theleft leg of her daughter, but she did not remember any febrile episode. She reported no othersymptoms or past medical history associated with skin ulcerations, diabetes, recurrent earinfections, or chronic diseases. In a few days, new lesions with similar characteristics appearedon her arms and legs. The first clinical impressions were ulcers caused by group A beta-hemolyticstreptococci or staphylococci. No bacteriological cultures or other microbiological studieswere performed to determine the putative cause of those lesions. Nevertheless, she received anempirical antibiotic treatment, such as topical rifampicin (3 applications\/day of a 100 mg\/mlsolution) concomitantly with penicillin G benzathine (1,200,000 UI, one dose, i.m.) cephalexin(500 mg orally every 12 h for 14 days) and ciprofloxacin (500 mg orally every 12 h for 7 days),which were administered sequentially due to clinical worsening of the skin lesions. After 2weeks, the physicians could not observe any lesion improvement after these treatments, andjudged by the evolution of lesions, they suspected a Leishmania infection and interrupted antibiotictreatment. The patient was transferred to the Hospital Pediatrico del Niño Jesus (children?shospital, Cordoba, Argentina) for an accurate diagnosis","palabras_clave":"LEISHMANIASIS|STREPTOCOCCUS|PNEUMONIAE|COINFECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5782,"idioma_id":4,"titulo":"Digital image processing techniques applied to pressure analysis and morphological features extraction in footprints","resumen":"This paper shows the correlation between foot morphology and pressure distribution on footplant by means of a morphological parameters analysis and pressure  calculation. Footprint images were acquired using an optical pedobarograph and then processed for obtaining binary masks and intensity images in gray scale. Morphological descriptors were obtained from the binary images and the Hernandez Corvo (HC) index was automatically calculated for determine the type of foot. Pressure distributions were obtained from gray scale images making a correspondence between light intensity in footprints and pressure. Pressure analysis was performed by finding the maximum pressure, the mean pressure and the ratio between them that determines the uniformity of the distribution. Finally, a high correlation was found between this ratio and the type of foot determined by HC index.","palabras_clave":"FOOTPRINT IMAGES|FEATURES EXTRACTION|HC INDEX|IMAGE PROCESSING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5783,"idioma_id":4,"titulo":"Analysis of genetic association in Listeria and Diabetes using Hierarchical Clustering and Silhouette Index","resumen":"It is usually assumed that co-expressed genes suggest co-regulation in the underlying regulatory network. Determining sets of co-expressed genes is an important task, where significative groups of genes are defined based on some criteria. This task is usually performed by clustering algorithms, where the whole family of genes, or a subset of them, areclustered into meaningful groups based on their expression values in a set of experiment.In this work we used a methodology based on the Silhouette index as a measure of cluster quality for individual gene groups, and a combination of several variants of hierarchical clustering to generate the candidate groups, to obtain sets of co-expressed genes for two real data examples.We analyzed the quality of the best ranked groups, obtained by the  algorithm, using an online bioinformatics tool that provides network information for the selected genes.Moreover, to verify the performance of the algorithm, considering the fact that it doesn?t find all possible subsets, we compared its results against a full search, to determine the amount of good co-regulated sets not detected.","palabras_clave":"CLUSTERING|IDICE SILHOUTTE|GENES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5784,"idioma_id":4,"titulo":"Acute hypotensive, diuretic and antioxidant activities induced by Urtica circularis","resumen":"Aims: The aim of this study was to investigate the possible hypotensive and diuretic effects ofethanolic extract of Urtica circularis (Hicken) Sorarú (Urticaceae) using preclinical methods.Place and Duration of Study: Department of Pharmacology, School of Pharmacy andBiochemistry, Universidad de Buenos Aires, from July 2015 to January 2016.Methodology: Effect on blood pressure and heart rate on anaesthetized normotensive andhypertensive rats were measured using Statham Gould P23ID pressure transducer coupled to aGrass 79D polygraph. Rats were placed in metabolic cages in order to collect urine. Urinary volumewas measured and sodium and potassium concentration was estimated from each urine sampleusing indirect ion-selective electrode potentiometry. The vasorelaxant activity of major compoundwas studied using isolated aortic rings. Antioxidant activity was estimated measuring 2,2 diphenyl 2picryl hydrazyl hydrate radical scavenging activity.Results: The intravenous administration of the extract of U. circularis (0.1?30 mg\/kg) anaesthetized normotensive and hypertensive rats caused a dose-dependent reduction in themean arterial pressure without affecting the heart rate. The greater reduction of blood pressureinduced by U. circularis was observed in hypertensive rats (30 mg\/kg: Spontaneously HypertensiveRat: -34.7±3.3 mmHg, Spague Dawley: -18.3±3.9 mmHg). Cumulative urinary excretions 24 h aftertreatment with the extract 100 and 300 mg\/kg were 18.2±1.2 and 14.9±1.5 mL respectively,significantly higher than the control group (9.0±1.3 mL). The addition of cumulative concentrationsof vicenin-2 (10-7-10-4M) generated relaxation in endothelium-intact aortic rings pre-contracted with10?7M Phenylephrine (Emax = 66.2±3.5%). Extract showed antioxidant activity reaching 45% ofDPPH scavenging activity at 1000 µg\/mL, meanwhile the flavonoid reached 20% of scavengercapacity.Conclusion: U. circularis, has a diuretic, antioxidant and hypotensive effect. Vicenin-2, the majorcomponent of this extract showed vasorelaxant activity, potentially responsible for the properties ofthe extract.","palabras_clave":"HYPOTENSIVE|DIURETIC|VASORELAXANT|ANTIOXIDANT|URTICA CIRCULARIS |VICENIN-2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5785,"idioma_id":4,"titulo":"Cytotoxic damage of soybean agglutinin (SBA) on intestinal epithelial cells of broiler chicks. In vitro protection by Bifidobacterium infantis CRL1395","resumen":"Plant lectins, which are proteins\/glycoproteins present in a wide range of vegetables, fruits, cereals and beans, are resistant to digestive enzymes and food cooking temperatures. They bind reversibly to specific glycosidic residues expressed on the membrane of intestinal epithelial cells (IEC) and cause anti-nutritional effects in humans and animals. Soybean lectin (SBA) has been detected in poultry diets, and its ability to bind to the intestinal epithelium has been reported. The development of new methods for removing SBA from feeds or to prevent interaction with the intestinal mucosa is of interest. In this study, the in vitro cytotoxicity of SBA on IEC of chicks was demonstrated for the first time. The LD50, assessed after 2 h exposure of IEC to SBA, was 6.13 &#956;g mL-1. The ability of Bifidobacterium infantis CRL1395 to bind SBA on the bacterial envelope was confirmed, and prevention of IEC cytotoxicity by lectin removal was demonstrated. Safety of B. infantis CRL1395, resistance to gastrointestinal stress and adhesion were also determined. It was concluded that the early administration of B. infantis CRL1395 to chicks would effectively reduce the toxicity of SBA. Besides, it would favour the colonisation of the gut with a beneficial microbiota.","palabras_clave":"SOYBEAN AGGLUTININ|CYTOTOXICITY|INTESTINAL EPITHELIAL CELLS|BROILER CHICKS|BIFIDOBACTERIUM|PROBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5786,"idioma_id":4,"titulo":"Feruloyl esterase activity is influenced by bile, probiotic intestinal adhesion and milk fat","resumen":"Cinnamoyl esterases (CE) are microbial and mammalian intestinal enzymes able to release antioxidant hydroxycinnamic acids from their non-digestible ester-linked forms naturally present in vegetable foods. Previous findings showed that oral administration of Lactobacillus  fermentum CRL1446 increased intestinal CE activity and improved oxidative status in mice. The aim of this work was to evaluate the in vitro CE activity of L. fermentum CRL1446 and the effect of bile on this activity, as well as strain resistance to simulated gastrointestinal tract (GIT) conditions and its ability to adhere to intestinal epithelium and influence its basal CE activity. L. fermentum CRL1446 and L. fermentum ATCC14932 (positive control for CE activity) were able to hydrolyse different synthetic hydroxycinnamates, with higherspecificity toward methyl ferulate (3853.73 and 899.19 U\/g, respectively). Feruloyl esterase (FE) activity was mainly intracellular in L. fermentum CRL1446 and cell-surface associatedin L. fermentum ATCC14932. Both strains tolerated simulated GIT conditions and were able to adhere ex vivo to intestinal epithelium. Pre-incubation of L. fermentum strains with bile increased FE activity in both whole cells and supernatants (~2-fold), compared to controls,suggesting that cells were permeabilised by bile, allowing more substrate to enter the celland\/or leakage of FE enzymes. Three-fold higher FE activities were detected in intestinaltissue fragments with adhered L. fermentum CRL1446 cells compared to control fragments (without bacteria), indicating that this strain provides exogenous FE activity and could stimulate esterase activity in the intestinal mucosa. Finally, we found that milk fat had a negative effect on FE activity of intestinal tissue, in absence or presence of adhered L. fermentum. These results help explaining the increase in intestinal FE activity previously observed in mice fed with L. fermentum CRL1446, and support the potential use of this strain for the development of new functional foods directed to oxidative stress-related ailments.","palabras_clave":"INTESTINAL ESTERASE ACTIVITY|LACTOBACILLUS FERMENTUM|ANTIOXIDANT HYDROXYCINNAMIC ACIDS|PROBIOTICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5787,"idioma_id":4,"titulo":"Measuring and using non-Markovianity","resumen":"We construct measures for the non-Markovianity of quantum evolution with a physically meaningful interpretation. We first provide a general setting in the framework of channel capacities and propose two families of meaningful quantitative measures, based on the largest revival of a channel capacity, avoiding some drawbacks of other non-Markovianity measures. We relate the proposed measures to the task of information screening. This shows that the non-Markovianity of a quantum process may be used as a resource. Under these considerations, we analyze two paradigmatic examples, a qubit in a quantum environment with classically mixed dynamics and the Jaynes-Cummings model.","palabras_clave":"non-markovianity|quantum open systems"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5788,"idioma_id":4,"titulo":"Chelating Properties of Peptides from Red Seaweed Pyropia columbina and Its Effect on Iron Bio-Accessibility","resumen":"The aim of this workwas to evaluate copper-chelating, iron-chelating and anticariogenic activity ofpeptides obtained by enzymatic hydrolysis of P. columbina proteinconcentrate and to study the effects of chelating peptides on ironbio-accessibility. Two hydrolyzates were obtained from P. columbina proteinconcentrate (PC) using two hydrolysis systems: alkaline protease (A) andalkaline protease + Flavourzyme (AF). FPLC gel filtration profile of PC shows apeak having molecular weight (MW) higher than 7000 Da (proteins).A and AF hydrolyzates had peptides with medium and low MW (1013 and 270 Da),respectively. Additionally,AF presented freeamino acids with MW around 82 Da and higher content of His and Ser. Peptidesfrom AF showed the highest chelating properties measured as copper-chelatingactivity (the lowest &#946;-caroteneoxidation rate: Ro;0.7 min&#8722;1),iron-chelating activity (33 %), and phosphorous and Ca2+ releaseinhibition (87 and 81 %, respectively). These properties could indicateantioxidant properties, promotion of iron absorptionand anticariogenicactivity, respectively. In fact, hydrolyzates promoted iron dialyzability (&#8776;16 %), values being higherthan that found for P. columbina seaweed. Chelating peptidesfrom both hydrolyzates can maintain the iron in a soluble and bio-accessibleform after gastrointestinal digestion.","palabras_clave":"CHELATING PEPTIDES |ANTICARIOGENIC PROPERTIES |IRON BIO-ACCESSIBILITY |RED SEAWEEDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5789,"idioma_id":5,"titulo":"Matrimonio igualitario, identidad de género y disputas por el derecho al aborto en Argentina. La política sexual durante el kirchnerismo (2003-2015)","resumen":"Los gobiernos ?progresistas? (pink tide) en materia socioeconómica del Cono Surno tienen la misma trayectoria en el avance de los derechos de las mujeres y colectivos LGBT. Lo distintivo de la política sexual de la Argentina (2003-2015) es la tensión entre el avance de la agenda de derechos de la diversidad sexual, con la aprobación de leyes como matrimonio igualitario e identidad de género, y los esfuerzos frustrados del feminismo por legalizar el derecho al aborto. Este artículo presenta un estudio comparativo de estos tres intentos de reformas normativas durante el período de gobierno conocido como kirchnerismo. El análisis empírico identifica cuatro dimensiones fundamentales que, en su combinación, abren oportunidades o generan resistencias al cambio normativo en políticas de género y sexualidad: los vínculos entre Poder Ejecutivo e Iglesia; la política de partidos en un sistema presidencialista;los marcos interpretativos de cada demanda; las formas organizativas y estrategias de las organizaciones que las promueven.","palabras_clave":"Matrimonio igualitario|Ley de Identidad de Género|Aborto|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5791,"idioma_id":4,"titulo":"Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups","resumen":"Biofilm-associated diseases account for 80% of all infections in humans. Due to the emergence of antibiotic resistances, alternative therapies such as Photodynamic Inactivation (PDI) of microorganisms have emerged. Porphyrins with intrinsic positive charges have been proposed as successful photosensitizers (PSs) against microorganisms. We have recently designed the new synthetic porphyrin 5,10,15,20-tetrakis[4-(3-N,N-dimethylammoniumpropoxy)phenyl]porphyrin (TAPP) containing four basic amine groups in the periphery of the tetrapyrrolic macrocycle, which can acquire positive charges at physiological pH, thus favouring the interaction with biomembranes.Illumination of planktonic cultures of Staphylococcus aureus at 180 J\/cm2 in the presence of 2.5 &#956;M TAPP induced complete bacteria eradication.For the TAPP-PDI treatment of S. aureus biofilms, higher light fluences and PS concentrations were needed. Employing 20 &#956;M TAPP and 180 J\/cm2, around 3-log CFU reduction were obtained.In order to determine the efficacy of TAPP-PDI on Gram-negative bacteria, we performed planktonic and biofilm assays employing Pseudomonas aeruginosa. Much higher TAPP doses as compared to S. aureus were needed to achieve planktonic bacteria photosensitization (3-log CFU reduction at 20 &#956;M TAPP and 180 J\/cm2). On the other hand, high concentrations of TAPP were nontoxic to P. aeruginosa growing on biofilms, and employing 30 &#956;M TAPP and 180 J\/cm2 we obtained 3-log CFU reduction.The main conclusion of the present work is that TAPP is a promising and efficient PS capable of promoting photodynamic killing of both Gram-negative and -positive in planktonic bacteria, though more effectively in the latter. In addition, TAPP-PDI induces similar photoinactivation rates in both bacteria types growing on biofilms, with lower dark toxicity in the Gram-negative one.","palabras_clave":"CHLORIN|PHOTOINACTIVATION|BIOFILMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5792,"idioma_id":4,"titulo":"Glacial melting: an overlooked threat to Antarctic krill","resumen":"Strandings of marine animals are relatively common in marine systems. However, the underlying mechanisms are poorly understood. We observed mass strandings of krill in Antarctica that appeared to be linked to the presence of glacial meltwater. Climate-induced glacial meltwater leads to an increased occurrence of suspended particles in the sea, which is known to affect the physiology of aquatic organisms. Here, we study the effect of suspended inorganic particles on krill in relation to krill mortality events observed in Potter Cove, Antarctica, between 2003 and 2012. The experimental results showed that large quantities of lithogenic particles affected krill feeding, absorption capacity and performance after only 24 h of exposure. Negative effects were related to both the threshold concentrations and the size of the suspended particles. Analysis of the stomach contents of stranded krill showed large quantities of large particles ( > 106&#956;m3), which were most likely mobilized by glacial meltwater. Ongoing climate-induced glacial melting may impact the coastal ecosystems of Antarctica that rely on krill.","palabras_clave":"Krill|Glacial melting|Climate change|Antarctica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5793,"idioma_id":4,"titulo":"Transport mechanism through metal-cobaltite interfaces","resumen":"The resistive switching (RS) properties as a function of temperature were studied for Ag\/La(1&#8722;x)SrxCoO3 (LSCO) interfaces. The LSCO is a fully relaxed 100&#8201;nm film grown by metal organic deposition on a LaAlO3 substrate. Both low and a high resistance states were set at room temperature, and the temperature dependence of their current-voltage (IV) characteristics was measured taking care to avoid a significant change of the resistance state. The obtained non-trivial IV curves of each state were well reproduced by a circuit model which includes a Poole-Frenkel element and two ohmic resistances. A microscopic description of the changes produced by the RS is given, which enables to envision a picture of the interface as an area where conductive and insulating phases are mixed, producing Maxwell-Wagner contributions to the dielectric properties.","palabras_clave":"Memoria resistiva|Conducción eléctrica|Poole-Frenkel|Efecto Maxwell-Wagner"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5794,"idioma_id":4,"titulo":"Manganite-based three level memristive devices with self-healing capability","resumen":"Physics Letters A 380 (2016) 2870?2875We report on non-volatile memory devices based on multifunctional manganites. The electric fieldinduced resistive switching of Ti\/La1\/3Ca2\/3MnO3\/n-Si devices is explored using different measurementprotocols. We show that using current as the electrical stimulus (instead of standard voltage-controlledprotocols) improves the electrical performance of our devices and unveils an intermediate resistancestate. We observe three discrete resistance levels (low, intermediate and high), which can be set eitherby the application of current?voltage ramps or by means of single pulses. These states exhibit retentionand endurance capabilities exceeding 104 s and 70 cycles, respectively. We rationalize our experimentalobservations by proposing a mixed scenario were a metallic filament and a SiOx layer coexist, accountingfor the observed resistive switching. Overall electrode area dependence and temperature dependentresistance measurements support our scenario. After device failure takes place, the system can beturned functional again by heating up to low temperature (120 &#9702;C), a feature that could be exploitedfor the design of memristive devices with self-healing functionality. These results give insight intothe existence of multiple resistive switching mechanisms in manganite-based memristive systems andprovide strategies for controlling them.","palabras_clave":"RRAM devices|resistive switching| manganites"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5795,"idioma_id":4,"titulo":". Breeding System Diversification and Evolution in American Poa supersect. Homalopoa (Poaceae: Poeae: Poinae).","resumen":"?Background and Aims Poa subgenus Poa supersect. Homalopoa has diversified extensively in the Americas. Over half of the species in the supersection are diclinous, most of these are from the New World, while a few are from SE Asia.  Diclinism in Homalopoa can be divided into three main types: gynomonoecism, gnodioecism, and dioecism. Here we expand the sampling of species of New World Homalopoa to date its origin and diversification in North and South America and examine the evolution and origin of the breeding system diversity. ?Methods A total of 124 specimens were included in the matrix of which 89 are species of Poa supersect. Homalopoa sections Acutifoliae, Anthochloa, Brizoides, Dasypoa, Dioicopoa, Dissanthelium, Homalopoa s.l., Madropoa and Tovarochloa, and the informal Punapoa group. Bayesian and Parsimony analyses were conducted on the datasets based on for markers: the nuclear ribosomal ITS and ETS, and plastid trnT-L and trnL-F. Dating analyses were performed on a reduced Poa matrix and enlarged Poaceae outgroup to utilize fossils as calibration points. A relaxed Bayesian molecular clock method was used.?Key Results Hermaphroditism appears to be pleisiomorphic in the monophyletic Poa supersect. Homalopoa, which is suggested to have originated in Eurasia 8.4-4.2 Mya. The ancestor of Poa supersect. Homalopoa radiated throughout the New World in the Late Miocene-Early Pliocene with major lineages originating during the Pliocene to Pleistocene (5-2 Mya). We infer at least three major pathways from hermaphroditism to diclinism in New World Homalopoa: two leading to dioecism, one via gynodioecism in South America and another directly from hermaphroditism in North America. A third pathway leads from hermaphroditism to gynomonoecism in Andean species of South America, with strictly pistillate species evolving in the highest altitudes. ?Conclusions Divergence dating provides a temporal context to the evolution of breeding systems in New World Poa supersect. Homalopoa. Our results are consistent with the infrageneric classification in part; we confirm monophyletic sections, propose to reclassify species of sect. Acutifoliae, Dasypoa, and Homalopoa s.l., and suggest that a new sectional treatment is needed for gynomonoecious species.","palabras_clave":"Breeding system|diclinism|dioecism|DNA|gynomonoecism|gynodioecism|molecular dating |molecular phylogeny|Poa|Homalopoa|South America|North America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5796,"idioma_id":4,"titulo":"Odor experiences during preimaginal stages cause behavioral and neural plasticity in adult honeybees","resumen":"In eusocial insects, experiences acquired during the development have long-termconsequences on mature behavior. In the honeybee that suffers profound changesassociated with metamorphosis, the effect of odor experiences at larval instars onthe subsequent physiological and behavioral response is still unclear. To address theimpact of preimaginal experiences on the adult honeybee, colonies containing larvaewere fed scented food. The effect of the preimaginal experiences with the food odorwas assessed in learning performance, memory retention and generalization in 3?5-and 17?19 day-old bees, in the regulation of their expression of synaptic-related genesand in theperception and morphology of their antennae. Three-five day old bees thatexperienced 1-hexanol (1-HEX) as food scent responded more to the presentationof the odor during the 1-HEX conditioning than control bees (i.e., bees reared incolonies fed unscented food). Higher levels of proboscis extension response (PER) to1-HEX in this group also extent to HEXA, the most perceptually similar odor to theexperienced one that we tested. These results were not observed for the group testedat older ages. In the brain of young adults, larval experiences triggered similar levelsof neurexins (NRXs) and neuroligins (Nlgs) expression, two proteins that have beeninvolved in synaptic formation after associative learning. At the sensory periphery, theexperience did not alter the number of the olfactory sensilla placoidea, but did reducethe electrical response of the antennae to the experienced and novel odor. Our studyprovides a new insight into the effects of preimaginal experiences in the honeybeeand the mechanisms underlying olfactory plasticity at larval stage ofQ6holometabolousQ14insects.","palabras_clave":"preimaginal odor experiences|behavior|neurobiology|electrophysiology|honeybee"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5797,"idioma_id":4,"titulo":"Carrot fiber (CF) composite films for antioxidant preservation: particle size effect","resumen":"The effect of particle size (53, 105 and 210 &#956;m) of carrot fiber (CF) on their hydration properties and antioxidant capacity as well as on the performance of the CF-composite films developed with commercial low methoxyl pectin (LMP) was studied. It was determined that CF contained carotenoids and phenolics co-extracted with polysaccharides (80%), rich in pectins (15%). CF showed antioxidant activity and produced homogeneous calcium-LMP-based composites. The 53-&#956;m-CF showed the lowest hydration capability and produced the least elastic and deformable composite film due probably to CF bridged by calcium-crosslinked LMP chains. Antioxidant activity associated to the loaded CF was found in composites. When L-(+)-ascorbic acid (AA) was also loaded, its hydrolytic stability increased with the decrease in CF-particle size, showing the lowest stability in the 0%-CF- and 210 &#956;m-CF-LMP films. Below &#8776;250 &#956;m, the particle size determined the hydration properties of pectin &#8722; containing CF, affecting the microstructure and water mobility in composites.","palabras_clave":"CARROT FIBER|COMPOSITE FILMS|PECTINS|CAROTENOIDS AND PHENOLICS|ANTIOXIDANT PRESERVATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5798,"idioma_id":4,"titulo":"Stability of L-(+)-ascorbic acid in alginate edible films loaded with citric acid for antioxidant food preservation","resumen":"Alginate of known copolymer composition was applied to antioxidant film developed by loading L-(+)-ascorbic (AA) and citric (CA) acids. Relative humidity (RH, 33.3?75.2%) of storage (25.0 °C) and glycerol (plasticizer) level, separately and through their interaction, decreased the AA hydrolytic stability and, hence, the antioxidant half-life of films. Glycerol can facilitate the water penetration from the environment into the polymeric network. The CA content did not vary during film storage. Film browning was then associated to AA degradation. However, CA slowed down the browning development at longer storage times because of the changes in the kinetic order. It may catalyze the formation of unstable color compounds. The present work permitted to determine that AA and CA were 100% recovered after film casting. Also, it allowed finding the adequate film composition and period where the alginate film supporting AA and CA can be used as an antioxidant active interface for food preservation.","palabras_clave":"CITRIC ACID|ASCORBIC ACID HYDROLYSIS|GLYCEROL|ALGINATE COPOLYMER|ANTIOXIDANT FILMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5799,"idioma_id":4,"titulo":"Effect of light on particulate and dissolved organic matter production of native and exotic macrophyte species in Patagonia","resumen":"Biological invasions are one of the main drivers of changes in ecological features in aquaticenvironments. Invasive alien species may have far-reaching effects on the host environment that need to be explored. In this study, we compared particulate and dissolved organic matter (POM and DOM) production from two common Patagonian wetland plants, one native (Eleocharis pachycarpa) and one exotic (Potentilla anserina), under different light conditions (exposed or not to ultraviolet radiation (UVR+, UVR-) and darkness). Our results indicated that when exposed to UVR both macrophytes differed in their POMproduction and DOM release: while in E. pachycarpa we observed an increase in the POM production P. anserina released significantly higher amount of DOM. The DOM produced by the exotic species showed lower photoreactivity, and had more colour than that produced by the native E. pachycarpa. The invasion by macrophytes may produce changes in the stoichiometric balance and ecological relationships that could modify the characteristics of wetland food web.","palabras_clave":"macrófitas|UVR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5800,"idioma_id":4,"titulo":"Magnetic monitoring of air pollution in Tandil city using the lichen Parmotrema Pilosum as bioindicator","resumen":"The lichen Parmotrema pilosum is sensitive to pollution and it can live accumulating airborne pollutants for long time, such characteristic allows its use as biomonitor for environmental mapping in urban areas when this epiphytic specie is available. In this work, we investigated the use of such passive collector andmagnetic techniques to monitor the air pollution in Tandil, a city located in Buenos Aires province with approximately 125,000 inhabitants, 60,000 vehicles and various metallurgical factories inside the urban area. The sampling strategy was carried out following a random stratified design and measuring magnetic susceptibility, magnetic hysteresis loops, anhysteretic and isothermal remanent magnetization and thermomagnetic studies to determine the magnetic properties of airborne particles accumulated on lichen samples. Scanning electron microscopy observations show particles with different morphologies (individual particles, spherules and aggregates) and composition (Fe, Al, Ni, Cr, Ti, Cu, K and Br) produced by metallurgical factories and by gaseous\/solid vehicle emissions. The magnetic mineralogy shows thepredominance of pseudo-single domain magnetite-like mineral and the magnetic grain size estimations indicate the presence of fine particles (&lt;0.1 um) in sites with low vehicular traffic or less polluted, while sites more affected by pollution (high vehicular traffic and metallurgical industries) are characterized by coarser magnetic grain size particles, between 0.1 and 5 um. Mass-specific magnetic susceptibility was represented in a 2-D contour map to observe in detail the distribution of magnetic particles in this urban area, giving high values (up to 1161.2 x10-8 m3 kg-1) that are indicative of areas with high pollution loading.","palabras_clave":"BIOMONITORING|MAGNETIC PROPERTIES|GRAIN SIZE|AIR POLLUTION|MAGNETITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5801,"idioma_id":4,"titulo":"NaA zeolite membranes synthesized on top of APTES-modified porous stainless steel substrates","resumen":"NaA zeolite membranes were synthesized on top of both disk and tubular porous stainless steel substrates by the secondary growth method. The support seed stage was optimized, considering the seed synthesis conditions and the deposition method. The formation of the NaA zeolite on both the membrane and the residual powder collected at the bottom of the vessel after synthesis was corroborated by X-ray diffraction (XRD). Scanning electron microscopy (SEM) revealed the formation of a film with well-defined crystals with a cubic morphology characteristic of the NaA zeolite. The support modification with 3-aminopropyltriethoxysilane improved the morphology and stability of the NaA zeolite membranes synthesized on top of porous stainless steel substrates. When vacuum was applied only in the last hydrothermal synthesis stage, a smooth zeolite layer was obtained on top of porous stainless steel supports. The single gas (H2, N2, CO2 and CH4) and mixture (H2\/N2 and H2\/CH4) perm-selective properties of the tubular membranes were evaluated as a function of temperature and pressure. Separation factors higher than the Knudsen coefficient were obtained in all membranes. The single gas H2 and CO2 permeation flux of the membranes increased with temperature indicating a preponderance of the activated diffusion over the Knudsen diffusion. The higher H2\/CO2 ideal separation factors were 7.6 and 5.6 for the more selective tubular membranes, at 453 K and 100 kPa.","palabras_clave":"HYDROGEN RECOVERY|NaA ZEOLITE|INORGANIC MEMBRANES|STAINLESS STEEL SUPPORT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5802,"idioma_id":4,"titulo":"Inhibition of Survival Pathways MAPK and NF-kB Triggers Apoptosis in Pancreatic Ductal Adenocarcinoma Cells via Suppression of Autophagy","resumen":"Background: Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with a survival rate of 4-6 months from the diagnosis. PDAC is the fourth leading cause of death among cancers in the western world. This incidence has a mortality rate of 10 cases per 100,000 population. Chemotherapy constitutes only a palliative strategy, with limited effects on life expectancy.  Aims: To investigate the biological response of PDAC to MAPK and NF-kB inhibitors and the role of autophagy in the modulation of these signaling pathways, results in a challenging work in order to improve medical protocols for patients with PDAC. Methods: Two ATCC cell lines, MIAPaCa-2 and PANC-1, were used as PDAC models. Cells were exposed to inhibitors of MAPK or NF-kB survival pathways alone or after autophagy inhibition. Several aspects were analyzed, cell proliferation, by [3H]TdR incorporation, cell death, assayed by TUNEL, regulation of autophagy by LC3-II level expression and modulation of pro-and anti-apoptotic proteins by western blot. Results: We demonstrated that the inhibition of survival pathways MAPK and NF-kB, with U0126 and CAPE (Caffeic Acid Phenethyl Ester), respectively, produced a strong inhibition of pancreatic tumor cells growth, without induction of apoptotic death. Interestingly, U0126 and CAPE induced apoptosis after autophagy inhibition in a caspase-dependent manner in MIAPaCa-2 cells and in a caspase-independent way in PANC-1 cells. Conclusions: Here, we present evidence that allows us to consider a combined therapy of an inhibitor of autophagy with an nhibitor of MAPK or NF-kB pathways, as a possible treatment regimen for pancreatic cancer.","palabras_clave":"Autophagy|Pancreatic Tumor|MAPK|NF-kB"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5804,"idioma_id":4,"titulo":"Malolactic fermentation induced by Lactobacillus plantarum in Malbec wines from Argentina","resumen":"The current market requirements to obtain wines with enhanced complexity and varietal character propose the finding of new microorganisms capable to dominate at an early stage of the vinification, to drive the malolactic fermentation (MLF) and to contribute to new and distinctive sensory profiles of the wines. During MLF malic acid naturally present in must is converted into lactic acid by a decarboxylation reaction driven by lactic acid bacteria, mainly Oenococcus oeni.  Although inoculation with O. oeni is widely used, this microorganism presents some limitations. Recently, a new strain of Lactobacillus plantarum suitable for MLF in high pH wines has been selected. This study evaluates the capacity of L. plantarum V22 starter culture to complete MLF at laboratory and semi-industrial scale in Malbec musts from Argentina. Malbec musts were co-inoculated with S. cerevisiae D254 and L. plantarum V22 to conduct alcoholic fermentation and MLF respectively. In semi-industrial experiments sequential inoculation with O. oeni VP41 was performed, as the reference MLF procedure used in the industry, to allow a comparison for the efficiency of malic acid degradation. The alcoholic fermentation was not affected by the co-inoculation strategy. Malic acid was partially or totally consumed in spite of a gradual decrease in L. plantarum viability in all conditions assessed. This study demonstrated the feasibility to use L. plantarum as a starter culture to conduct MLF in Malbec musts, additionally suggesting that the successful completion of MLF may be associated with pH evolution during the fermentation process, rather than to initial must pH.","palabras_clave":"MALOLACTIC FERMENTATION|LACTOBACILLUS PLANTARUM|MALBEC|WINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5805,"idioma_id":4,"titulo":"Alternative Splicing of G9a Regulates Neuronal Differentiation","resumen":"Chromatin modifications are critical for the establishment and maintenance of differentiation programs. G9a, the enzyme responsible for histone H3 lysine 9 dimethylation in mammalian euchromatin, exists as two isoforms with differential inclusion of exon 10 (E10) through alternative splicing. We find that the G9a methyltransferase is required for differentiation of the mouse neuronal cell line N2a and that E10 inclusion increases during neuronal differentiation of cultured cells, as well as in the developing mouse brain. Although E10 inclusion greatly stimulates overall H3K9me2 levels, it does not affect G9a catalytic activity. Instead, E10 increases G9a nuclear localization. We show that the G9a E10(+) isoform is necessary for neuron differentiation and regulates the alternative splicing pattern of its own pre-mRNA, enhancing E10 inclusion. Overall, our findings indicate that by regulating its own alternative splicing, G9a promotes neuron differentiation and creates a positive feedback loop that reinforces cellular commitment to differentiation.","palabras_clave":"Alternative Splicing|Neuronal Differentiation|Histone methyltransferase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5806,"idioma_id":5,"titulo":"Los Tardigrada (Mammalia, Xenarthra) de Argentina: diversidad, evolución y biogeografía","resumen":"Los Tardigrada representan uno de los grupos de mamíferos fósiles característicos del Cenozoico de América del Sur, pero que también se encuentra representado en América Central y América del Norte. El registro fehaciente más antiguo del grupo correspondería a Pseudoglyptodon del Eoceno tardío de Chubut, y en la actualidad está representado por los géneros arborícolas Bradypus y Choloepus. El registro fósil da cuenta de una gran diversidad taxonómica de tardígrados, con más de 50 géneros bien conocidos, con formas arborícolas, semiarborícolas y cursoriales. Tardigrada incluye cuatro clados principales: Megatheriidae, Megalonychidae, Nothrotheriidae y Mylodontidae, a los que suma una serie de géneros conocidos como Megatherioidea basales. A partir del Oligoceno tardío se hacen abundantes y están representados por varias líneas de Megatherioidea y Mylodontoidea. Durante el Neógeno (Mioceno?Plioceno) el grupo alcanza una alta diversidad con representantes de todas las familias. Durante el Cuaternario algunos géneros alcanzan los mayores tamaños (e.g., Megatherium, Lestodon) y forman parte de las asociaciones conocidas como megafauna. Hacia el final del Pleistoceno e inicio del Holoceno las formas de perezosos de gran tamaño se extinguen junto con otros elementos de la megafauna.","palabras_clave":"AMÉRICA DEL SUR |XENARTHRA|PILOSA|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5807,"idioma_id":5,"titulo":"EDIFICIOS EN ALTURA MÁSICOS Y LIVIANOS EN CIUDADES-OASIS. EVALUACIÓN TÉRMICA Y ENERGÉTICA DE VIVIENDAS DEBAJO Y SOBRE LA COPA DE LOS ÁRBOLES EN MENDOZA, ARGENTINA.","resumen":"La forestación urbana en Ciudades-Oasis definen desde el punto de vista ambiental dos estratos en altura: una situación micro-climática moderada debajo la copa de los árboles y una situación extrema sobre la misma. El objetivo del trabajo es evaluar la eficiencia energética y las condiciones de habitabilidad de unidades de viviendas en edificios en altura. Se seleccionaron cuatro casos en la Ciudad-Oasis de Mendoza, Argentina: dos ubicados debajo y dos sobre la copa de los árboles. Se evaluaron envolventes del tipo másica y liviana. Se realizaron durante dos años mediciones higro-térmicas in situ utilizadas para validar modelos mediante el programa de simulación Energy Plus. Se estudiaron medidas de rehabilitación energética y se analizaron comportamientos térmicos y consumos de energía para climatización. Los resultados demuestran que los departamentos debajo la copa de los árboles aprovechan en verano la protección de la arboleda urbana a la radiación incidente (diferencias térmicas del orden de 4°C), y cuentan con temperaturas más elevadas en invierno (diferencias del orden de 3°C). Con la materialidad edilicia mejorada, debajo la copa se logran consumos para climatización mínimos, cercanos a un edificio de energía cero. Asimismo, las viviendas de los edificios másicos demuestran comportamientos térmicos más cercanos al rango de confort respecto a los edificios livianos.","palabras_clave":"CIUDADES-OASIS|BAJO Y SOBRE LA COPA DE LOS ÁRBOLES|EDIFICIOS EN ALTURA|EFICIENCIA ENERGÉTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5808,"idioma_id":4,"titulo":"Acetaminophen from liver to brain: New insights into drug pharmacological action and toxicity","resumen":"Acetaminophen (APAP) is a well-known analgesic and antipyretic drug. It is considered to be safe whenadministered within its therapeutic range, but in cases of acute intoxication, hepatotoxicity can occur.APAP overdose is the leading cause of acute liver failure in the northern hemisphere. Historically, studieson APAP toxicity have been focused on liver, with alterations in brain function attributed to secondaryeffects of acute liver failure. However, in the last decade the pharmacological mechanism of APAP as acannabinoid system modulator has been documented and some articles have reported ?in situ? toxicityby APAP in brain tissue at high doses. Paradoxically, low doses of APAP have been reported to producethe opposite, neuroprotective effects. In this paper we present a comprehensive, up-to-date overview ofhepatic toxicity as well as a thorough review of both toxic and beneficial effects of APAP in brain.","palabras_clave":"Acetaminophen  |Hepatic toxicity|trpv1 Necroptosis|Caspase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5809,"idioma_id":4,"titulo":"Comparative analysis of the morphology and histochemistry of the duodenum of the coypu (Myocastor coypus bonariensis) during its prenatal and postnatal development","resumen":"The objective of this study focused on the comparative morphological and histochemical analysis of the duodenum of fetuses, juveniles and adult coypu (Myocastor coypus bonariensis), the major socioeconomic wildlife resource of Argentina. Histological and histochemical procedures for in situ characterization of glycoconjugates (GCs) were used. This study evidenced that fetal mucins differ histochemically in many respects from their adult counterparts. Only in fetuses from 90 days-post coitus (dpc) glycogen-rich sites were observed throughout the duodenal epithelium. The goblet cells appeared from 105 dpc and their secretory content varied considerably after birth. Duodenal glands presented scanty neutral and sulphated GCs in the 30-day juveniles; in adults the proportion of these GCs increased, and carboxylated and sialylated GCs were also observed. The results obtained in this work may be used in future studies to evaluate the effects of diet and intestinal pathologies in the glycosylation pattern of GCs. Also, knowledge of the normal glycoprofile of the duodenum of M. coypus bonariensis during its ontogenetic development may constitute a basis for the study of this organ in other Hystricognathi rodents.","palabras_clave":"HYSTRICOGNATHI|MYOCASTOR COYPUS BONARIENSIS|DUODENUM DEVELOPMENT|HISTOCHEMISTRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5810,"idioma_id":4,"titulo":"Comparative analysis of the morphology, ultrastructure and glycosylation pattern of the jejunum and ileum of the wild rodent Lagostomus maximus","resumen":"Morphological and histochemical analyses were performed to characterizethe histology, ultrastructure, and glycosylation pattern of the jejunumand ileum of the wild rodent Lagostomus maximus. Enterocytes, goblet cells, Paneth cells, and enteroendocrine cells were identified in both intestinal epithelia. Two morphological types of enterocytes were identified only in the ileum based on their cytoplasm electron density. Although the histological and ultrastructural examination showed that the epithelia of both anatomical regions were morphologically similar, a certain specialization in their secretory products was evident. The glycosylation pattern of the jejunum and ileum was characterized in situ by histochemical and lectin histochemical methods. Histochemical results revealed the presence of carboxylated and sulfated gycoconjugates in both regions, although sulfomucins were clearly prevalent in the ileum. Sialic acid was highly O-acetylated and particularly abundant in the jejunum. The KOH\/PA*\/Bh\/PAS technique evidenced a more intense histochemicalreaction in the jejunal than in the ileum goblet cells, demonstrating areduction of neutral mucin secretion in the distal small intestine. Furtherspecific differences were revealed by lectin histochemistry. These data evidenced that the nature of mucus varies at different anatomical regions,probably adapted to physiological requirements.","palabras_clave":"LAGOSTOMUS MAXIMUS|SMALL INTESTINE|ULTRASTRUCTURE|MORPHOLOGY|GLYCOPATTERN ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5811,"idioma_id":5,"titulo":"Efecto de la administración de eCG 14 días posIATF sobre el porcentaje de preñez en vacas con cría","resumen":"El objetivo del presente trabajo fue estudiar el efecto de la administración de eCG al día 14 pos IATF sobre el porcentaje de preñez. Se utilizaron 305 vacas, de segundo servicio, cruza Bos indicus x Bos taurus con ternero al pie (condición corporal: 2,5±0,2, rango: 2-3 en la escala del 1 a 5). A 108 animales se les determinó el estado reproductivo (ER) por palpación transrrectal. El día 0 se colocó un dispositivo intravaginal con 0,5 g de progesterona y se aplicó una inyección de 2 mg de benzoato de estradiol. El día 7, se retiró el dispositivo y se administraron 0,5 mg de D (+) cloprostenol, 0,5 mg de cipìonato de estradiol y 400 UI de eCG por vía intramuscular. Entre las 50 y 54 horas posteriores se realizó la IATF con semen congelado\/descongelado en pajuelas de 0,5 ml de dos toros de probaba fertilidad. El día 21 (14 días pos IATF) las vacas fueron asignadas aleatoriamente para recibir o no 400 UI de eCG. El diagnóstico de gestación se realizó a los 45 días posIATF mediante palpación transrrectal. Se estudió el efecto del tratamiento (con y sin eCG en el día 14 pos IATF), del toro, del ER y de las interacciones sobre el porcentaje de preñez. El uso de la eCG en el día 14 pos IATF no tuvo efecto sobre el porcentaje de preñez (eCG: 60,1%; Control: 56,5%); no existiendo efecto del toro, del ER, ni de sus interacciones (P>0,05). En conclusión, en las condiciones del presente trabajo, la administración de 400 UI de eCG en el día 14 pos IATF no produce ningún efecto sobre el porcentaje de preñez en vacas con cría al pie que presentan una condición corporal promedio por debajo de la ideal para la aplicación de dicha técnica.","palabras_clave":"PREÑEZ|IATF|ECG|ESTADO REPRODUCTIVO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5812,"idioma_id":4,"titulo":"Transgenic plants and hairy roots: exploiting the potential of plant species to remediate contaminants","resumen":"Phytoremediation has emerged as an attractive methodology to deal with environmental pollution,which is a serious worldwide problem. Although important advances have been made in this researchfield, there are still some drawbacks to become a widely used practice, such as the limited plant?smetabolic rate and their difficulty to break down several organic compounds or to tolerate\/accumulateheavy metals. However, biotechnology has opened new gateways in phytoremediation research byoffering the opportunity for direct gene transfer to enhance plant capabilities for environmentalcleanup. In this context, hairy roots (HRs) have emerged as an interesting model system to explore thepotential of plants to remove inorganic and organic pollutants. Besides, their use in rhizoremediationstudies has also been explored. In this minireview we will discuss the most recent advances using geneticengineering for enhancing phytoremediation capabilities of plants and HRs.","palabras_clave":"TRANSGENIC PLANT|HAIRY ROOTS|PHYTOREMEDIATION|CONTAMINANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5813,"idioma_id":4,"titulo":"Effect of estradiol cypionate and GnRH treatment on plasma estradiol-17&#946; concentrations, synchronization of ovulation and on pregnancy rates in suckled beef cows treated with FTAI based protocols","resumen":"Two experiments were conducted to evaluate the effect of different ovulation inducers on E-17&#946; plasma concentrations, synchronized ovulations and pregnancy rates. In Experiment 1, cows received a progesterone intravaginal device (PID) with 1 g of progesterone (P4) plus 2 mg of estradiol benzoate (EB) (day 0). At PID removal (day 8), cows received 0.150 mg of D-cloprostenol and were randomly assigned to four treatment groups (n = 10\/treatment): Group ECP: 1 mg of estradiol cypionate at PID removal, Group EB: 1 mg of EB 24 hr after PID removal, Group GnRH: 10 &#956;g of GnRH 48 hr after PID removal, Group ECP-GnRH:1 mg of ECP at PID removal plus 10 &#956;g of GnRH 48 hr later. Ultrasonographic examinations were performed to detect the dominant follicle and ovulation. GnRH-treated cows ovulated later (p  .05; ECP: 54.0%, 68\/126; EB: 49.2%, 62\/126; GnRH: 40.4%, 55\/136; ECP+GnRH: 43.9%, 61\/139). In conclusion, ECP administration (ECP and ECP+GnRH treatments) affected E-17&#946; concentrations, determining its earlier increase and later decrease compared to treatments without ECP (EB and GnRH treatments). ECP+GnRH-treated cows achieved the best distribution of ovulations without affecting pregnancy rates.","palabras_clave":"Estradiol cypionate|GnRH|Ostradiol-17beta|FTAI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5814,"idioma_id":4,"titulo":"Gasotransmitters and Stomatal Closure: Is There Redundancy, Concerted Action, or Both?","resumen":"The epidermis of the aerial part of land plants is pierced by pores through which plants perform gas exchange with environment. The guard cells (GCs), the specialized cells that surround the pore, have the capacity to sense diverse environmental and endogenous stimuli and integrate them into a single output which is the regulation of the stomatal pore width. The stomatal pore size is modulated by changes of the guard cell volume, driven by variations in the osmotic potential of the GCs.","palabras_clave":"SUFLFURO DE HIDROGENO|ESTOMAS|TRANSDUCCION DE SEÑALES EN PLANTAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5815,"idioma_id":5,"titulo":"Control de la erosion eólica con un estabilizador artificial en un Torripsammente ústico","resumen":"La erosión eólica es uno de los principales procesos de degradación de suelos de ambientes áridos y semiáridos. Una alternativade control de este proceso es la estabilización del suelo por medio de fijadores artificiales. En el presente estudio se analizó elgrado de control de la erosión que produjo un estabilizador a base de acetato de polivinilo (cola vinílica), diluido en agua a distintasconcentraciones. El estudio se realizó en un túnel de viento en un Torripsament Ustico. Se utilizaron tres concentraciones(0,5, 2 y 10% relación estabilizador-agua) con y sin inyección de la fracción de saltación, dos momentos de muestreo (5 y10 minutos) y dos velocidades de viento (10,5 y 12 m s-1). Los resultados indicaron que la erosión fue significativamente menoren todos los tratamientos con agregado del estabilizador que en el suelo sin estabilizador. La erosión con concentraciones delestabilizador de 2 y 10% fueron menores (p<0,05) que a 0,5%, en los tratamientos con y sin saltación. El estabilizador aplicadoal 2 y 10% mantuvo bajos niveles de erosión en ambos momentos de muestreo, lo que indica que la costra formada sobrela superficie del suelo es resistente al efecto abrasivo de las partículas de saltación. Se pudo deducir que una aplicación delestabilizador a una concentración del 2% permite controlar eficientemente la erosión en el suelo analizado, manteniendo laspérdidas de suelo en niveles bajos, durante al menos 10 minutos, aún con altas velocidades del viento. Debido a que el estabilizadores de bajo costo, posee bajo impacto ambiental y se encuentra disponible en el mercado, su uso para controlar la erosión eólicaen pequeñas superficies, con fines ingenieriles en obras de construcción, estabilización de banquinas y superficies de caminos,es potencialmente factible.","palabras_clave":"Erosión eólica|métodos de control|estabilizador sintético"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5816,"idioma_id":4,"titulo":"Alteration of syncytiotrophoblast mitochondria function and endothelial nitric oxide synthase expression in the placenta of rural residents","resumen":"tThe impact of environmental organophosphate (OP) pesticide exposure on respiratory complexes, enzy-matic antioxidant defense activities, and oxidative damage markers in the syncytiotrophoblast andcytotrophoblast mitochondria was evaluated. Placental progesterone (PG) levels and endothelial nitricoxide synthase (eNOS) expression were studied. Samples from women non-exposed (control group-CG)and women living in a rural area (rural group-RG) were collected during pesticide spraying season (RG-SS)and non-spraying season (RG-NSS).In RG-SS, the exposure biomarker placental carboxylesterase decreased and syncytiotrophoblastcytochrome c oxidase activity increased, while 4-hydroxynonenal levels decreased. PG levels decreasedin RG-SS and in the RG. Nitric oxide synthase expression decreased in RG, RG-SS and RG-NSS. No signifi-cant changes in mitochondrial antioxidant enzyme activities were found. These results suggest that thealteration of syncytiotrophoblast mitochondrial complex IV activity and steroidogenic function may beassociated to pesticide exposure. Reduction in placental PG and eNOS expression may account for lownewborn weight in RG.","palabras_clave":"ORGANOPHOSPHATE PESTICIDES |SYNCYTIOTROPHOBLAST MITOCHONDRIA|PROGESTERONE|ENDOTHELIAL NOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5817,"idioma_id":4,"titulo":"Current and future levels of mercury atmospheric pollution on global scale","resumen":"An assessment of current and future emissions, air concentrations and atmospheric deposition of mercury world-wide are presented on the basis of results obtained during the performance of the EU GMOS (Global Mercury Observation System) project. Emission estimates for mercury were prepared with the main goal of applying them in models to assess current (2013) and future (2035) air concentrations and atmospheric deposition of this contaminant. The artisanal and small- scale gold mining, as well as combustion of fossil fuels (mainly coal) for energy and heat production in power plants and in industrial and residential boilers are the major anthropogenic sources of Hg emissions to the atmosphere at present. These sources account for about 37 % and 25 % of the total anthropogenic Hg emissions globally, estimated to be about 2000 tonnes. The emissions in Asian countries, particularly in China and India dominate the total emissions of Hg. The current estimate of mercury emissions from natural processes (primary mercury emissions and re-emissions), including mercury depletion events, were estimated to be 5207 tonnes per year which represent nearly 70 % of the global mercury emission budget. Oceans are the most important sources (36 %) followed by biomass burning (9 %). A comparison of the 2035 anthropogenic emissions estimated for 3 different scenarios with current anthriopogenic emissions indicates a reduction of these emissions in 2035 up to 85 % for the best case scenario. Two global chemical transport models (GLEMOS and ECHMERIT) have been used for the evaluation of future Hg pollution levels considering future emission scenarios. Projections of future changes in Hg deposition on a global scale simulated by these models for three anthropogenic emissions scenarios of 2035 indicate a decrease of up to 50 % deposition in the Northern Hemisphere and up to 35 % in Southern Hemisphere for the best case scenario. The EU GMOS project has proved to be a very important research instrument for supporting, first the scientific justification for the Minamata Convention, and then monitoring of the implementation of targets of this Convention, as well as, the EU Mercury Strategy. This project provided the state-of-the art with regard to the development of the latest emission inventories for mercury, future emission scenarios, dispersion modelling of atmospheric Hg on global and regional scale, and source ? receptor techniques for Hg emission apportionment on a global scale.","palabras_clave":"Mercury deposition|GMOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5819,"idioma_id":4,"titulo":"Spatial variability of chromophoric dissolved organic matter in a large floodplain river: control factors and relations with phytoplankton during a low water period","resumen":"Chromophoric dissolved organic matter (CDOM) affects ecological processes in freshwater environments. Few studies assessed its spatial variability and relations with phytoplankton in floodplain rivers. Therefore, these topics were examined in a hydrological connectivity gradient in the Middle Paraná system. Absorption coefficients at 250 and 365&#8201;nm were measured to estimate CDOM concentration and molecular weight (MW), to find their explanatory limnological variables (Redundancy Analysis, RDA), and to assess their contribution to the explanation of phytoplankton structure (Canonical Correspondence Analysis, CCA). Conductivity and turbidity explained CDOM significantly. Increased conductivity from the main channel to floodplain lakes was associated with increased CDOM concentration. The lake indirectly connected to the river showed the highest turbidity associated with high-MW-CDOM, while the isolated lake showed the highest conductivity associated with low-MW-CDOM. Phytoplankton structure was significantly explained by conductivity, turbidity, and high-MW-CDOM (CCA). Environments directly connected to the river (with the lowest conductivity) were associated with diatoms. Phytoflagellates were associated with turbidity and high-MW-CDOM in the lake indirectly connected to the river, whereas Cyanobacteria were associated with conductivity and low-MW-CDOM in the isolated lake. The combined effect of physical factors and CDOM explained the highest fraction of species variation (partial CCA). As a conclusion: (1) CDOM increases as hydrological connectivity with the river decreases; (2) lake sediment resuspension is linked to an increase of high-MW-CDOM; (3) lake isolation from the river corresponds to an increase in low-MW-CDOM; (4) CDOM explains phytoplankton structure significantly and should be considered as an important variable in studies of microalgal assembly.","palabras_clave":"HYDROLOGICAL CONNECTIVITY|CDOM OPTICAL ESTIMATORS|LIMNOLOGICAL VARIABLES|PHYTOPLANKTON STRUCTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5820,"idioma_id":4,"titulo":"A novel gel based on an ionic complex from a dendronized polymer and ciprofloxacin: Evaluation of its use for controlled topical drug release","resumen":"The development and characterization of a novel, gel-type material based on a dendronized polymer (DP) loaded with ciprofloxacin (CIP), and the evaluation of its possible use for controlled drug release, are presented in this work. DP showed biocompatible and non-toxic behaviors in cultured cells, both ofwhich are considered optimal properties for the design of a final material for biomedical applications. These results were encouraging for the use of the polymer loaded with CIP (as a drug model), under gel form, in the development of a new controlled-release system to be evaluated for topical administration. First, DP-CIP ionic complexes were obtained by an acid-base reaction using the high density of carboxylic acid groups of the DP and the amine groups of the CIP. The complexes obtained in the solid state were broadly characterized using FTIR spectroscopy, XRP diffraction, DSC-TG analysis and optical microscopy techniques. Gels based on the DP-CIP complexes were easilyprepared and presented excellent mechanical behaviors. In addition, optimal properties for application on mucosal membranes and skinwere achieved due to their high biocompatibility and acute skin non-irritation. Slow and sustained release of CIP toward simulated physiological fluids was observed in the assays (in vitro), attributed toion exchange phenomenon and to the drug reservoir effect. An in vitro bacterial growth inhibition assay showed significant CIP activity, corresponding to 38 and 58% of that exhibited by a CIP hydrochloride solution at similar CIP concentrations, against Staphylococcus aureus and Pseudomonas aeruginosa, respectively. However, CIP deliverywas appropriate, both in terms of magnitude and velocity to allow for a bactericidal effect. In conclusion, the final product showed promising behavior, which could be exploited for the treatment of topical and mucosal opportunistic infections in human or veterinary applications.","palabras_clave":"DENDRONISED POLYMER|HYDROGEL|CIPROFLOXACIN|POLYELECTROLYTE-DRUG COMPELXES|CONTROLLED RELEASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5821,"idioma_id":4,"titulo":"Timeless standards for species delimitation","resumen":"ver texto completo","palabras_clave":"General"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5822,"idioma_id":4,"titulo":"A whole genome bioinformatic approach to determine potential latent phase specific targets in Mycobacterium tuberculosis","resumen":"Current Tuberculosis treatment is long and expensive, faces the increasing burden of MDR\/XDR strains and lack of effective treatment against latent form, resulting in an urgent need of new anti-TB drugs. Key to TB biology is its capacity to fight the host?s RNOS mediated attack. RNOS are known to display a concentration dependent  mycobactericidal activity, which leads to the following hypothesis ?if we know which proteins are targeted by RNOS and kill TB, we we might be able to inhibit them with drugs resulting in a synergistic bactericidal effect?. Based on this idea, we performed an Mtb metabolic network whole proteome analysis of potential RNOS sensitive and relevant targets which includes target druggability and essentiality criteria. Our results, available at http:\/\/tuberq.proteinq.com.ar yield new potential TB targets, like I3PS, while also providing and updated view of previous proposals becoming an important tool for researchers looking for new ways of killing TB.","palabras_clave":"Mycobacterium tuberculosis|Reactive oxygen and nitrogen species|Latent Phase|Structural Bioinformatics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5823,"idioma_id":4,"titulo":"Mechanism of the Reaction of Human Manganese Superoxide Dismutase with Peroxynitrite: Nitration of Critical Tyrosine 34","resumen":"Human Mn-containing superoxide dismutase (hMnSOD) is amitochondrial enzyme that metabolizes superoxide radical (O2?&#8722;). O2?&#8722; reacts atdiffusional rates with nitric oxide to yield a potent nitrating species, peroxynitriteanion (ONOO&#8722;). MnSOD is nitrated and inactivated in vivo, with active siteTyr34 as the key oxidatively modified residue. We previously reported a k of &#8764;1.0× 105 M&#8722;1 s&#8722;1 for the reaction of hMnSOD with ONOO&#8722; by direct stopped-flowspectroscopy and the critical role of Mn in the nitration process. In this study, wefurther established the mechanism of the reaction of hMnSOD with ONOO&#8722;,including the necessary re-examination of the second-order rate constant by anindependent method and the delineation of the microscopic steps that lead to theregio-specific nitration of Tyr34. The redetermination of k was performed bycompetition kinetics utilizing coumarin boronic acid, which reacts with ONOO&#8722;at a rate of &#8764;1 × 106 M&#8722;1 s&#8722;1 to yield the fluorescence product, 7-hydroxycoumarin. Time-resolved fluorescence studies in the presence of increasing concentrations of hMnSOD provided a kof &#8764;1.0 × 105 M&#8722;1 s&#8722;1, fully consistent with the direct method. Proteomic analysis indicated that ONOO&#8722;, but not othernitrating agents, mediates the selective modification of active site Tyr34. Hybrid quantum-classical (quantum mechanics\/molecular mechanics) simulations supported a series of steps that involve the initial reaction of ONOO&#8722; with MnIII to yield MnIVand intermediates that ultimately culminate in 3-nitroTyr34. The data reported herein provide a kinetic and mechanistic basis forrationalizing how MnSOD constitutes an intramitochondrial target for ONOO&#8722; and the microscopic events, with atomic levelresolution, that lead to selective and efficient nitration of critical Tyr34.","palabras_clave":"MnSOD|nitration"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5824,"idioma_id":4,"titulo":"Coarse-Grained Simulations of Heme Proteins: Validation and Study of Large Conformational Transitions","resumen":"Heme proteins are ubiquitous in nature and perform many diverse functions in all kingdoms of life. Many of thesefunctions are related to large-scale conformational transitions and allosteric processes. Sampling of these large conformationalchanges is computationally very challenging. In this context, coarse-grain simulations emerge as an efficient approach to explorethe conformational landscape. In this work, we present a coarse-grained model of the heme group and thoroughly validate thismodel in different benchmark examples, which include the monomeric heme proteins myoglobin and neuroglobin and thetetrameric human hemoglobin where we evaluated the method?s ability to explore conformational changes (as the formation ofhexacoordinated species) and allosteric transitions (as the well-known R &#8594; T transition). The obtained results are comparedwith atomistic molecular dynamics simulations. Overall, the results indicate that this approach conserves the essential dynamicalinformation on different allosteric processes.","palabras_clave":"coarse grain|heme protein"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5825,"idioma_id":4,"titulo":"Assessment of pathogenicity of natural IGFALS gene variants by in silico bioinformatics tools and in vitro functional studies","resumen":"Acid-labile subunit (ALS) is essential for stabilization of IGF-I and IGFBP-3 internary complexes within the vascular system. ALS deficient (ALS-D) patients and a subsetof children with idiopathic short stature (ISS), presenting IGFALS gene variants, showvariable degree of growth retardation associated to IGF-I and IGFBP-3 deficiencies. Theaim of this study was to evaluate the potential pathogenicity of eleven IGFALS variantsidentified in ALS-D and ISS children using in silico and in vitro approaches. We were ableto classify seven of these variants as pathogenic since they present impaired synthesis(p.Glu35Lysfs*87, p.Glu35Glyfs*17, p.Asn276Ser, p.Leu409Phe, p.Ser490Trp andp.Cys540Arg), or partial impairment of synthesis and lack of secretion (p.Leu213Phe). Wealso observed significant reduction of secreted protein for variants p.Ala330Asp,Ala475Val and p.Arg548Trp, while still retaining their ability to form ternary complexes.These findings provide an approach to test the pathogenicity of IGFALS gene variants.","palabras_clave":"IGFALS gene variants |Idiopathic short stature|IGF-I deficiency |Acid-labile subunit"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5826,"idioma_id":4,"titulo":"First reports of the invasive pest Bermudagrass Stem Maggot, Atherigona reversura Villeneuve, 1936 (Diptera: Muscidae) in South America","resumen":"This study presents the first occurrence of Atherigona reversura in South America. This muscid, commonly known as shoot-fly, is a significant pest of cereal crops throughout the Old World tropics and subtropics. Several specimens were collected during various months in 2014 and 2015. These new records are dispersed in a wide geographical area of temperate and subtropical regions of eastern Argentina. The main host of A. reversura, the exotic bermudagrass Cynodon dactylon, is considered a pest and is widely distributed in South America.","palabras_clave":"PEST CROPS|EXOTIC SPECIES|MUSCOID|FLY|WORLD DISTRIBUTION|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5827,"idioma_id":4,"titulo":"Interleukin-6 receptor in spindle-shaped stromal cells, a prognostic determinant of early breast cancer","resumen":"Mail of the Editorial Office-Tumor Biology (June 15-2016)-----From: Editorial Office Tumor Biology Date: 2016-06-15 8:08 GMT-03:00Subject: Your Submission TUBI-D-16-01204R1To: NA Chasseing <alejachase@gmail.com-------Dear Prof. Chasseing,We are pleased to inform you that your manuscript, 'Interleukin-6 receptor in spindle-shaped stromal cells, a prognostic determinant of early breast cancer', has been accepted for publication inTumor Biology.With best regards,Torgny Ingemar Stigbrand, Ph.D., MD.,ProfEditor in Chief---------------------------------------------------Abstract: Spindle-shaped stromal cells, like carcinoma associated fibroblasts and mesenchymal stem cells, influence tumor behavior and can serve as parameters in the clinical diagnosis, therapy, and prognosis of early breast cancer. Therefore, the aim of this study is to explore the clinicopathological significance of TRAIL-R2 and R4, as well as IL-6R in spindle-shaped stromal cells, not associated with the vasculature, as prognostic determinants of early breast cancer patients. Receptors are able to trigger the migratory activity, among other functions, of these stromal cells. We conducted immunohistochemical analysis for the expression of these receptors in spindle-shaped stromal cells, not associated with the vasculature, of primary tumors from early invasive breast cancer patients, and analyzed their association with clinicopathological characteristics. Here, we demonstrate that the elevated levels of TRAIL-R2, TRAIL-R4 and IL-6R in these stromal cells were significantly associated with a higher risk of metastatic occurrence (p=0.034, 0.026 and 0.006; respectively). Moreover, high expression of TRAIL-R4 was associated with shorter disease-free survival and metastasis-free survival (p=0.013 and 0.019; respectively). Also, high expression of IL-6R was associated with shorter disease-free survival, metastasis-free survival and overall survival (p=0.003, 0.001 and 0.003; respectively). Multivariate analysis showed that IL-6R expression was an independent prognostic factor for disease-free survival and metastasis-free survival (p=0.035). This study is the first to demonstrate that high levels of IL-6R expression in spindle-shaped stromal cells, not associated with the vasculature, could be used to identify early breast cancer patients with poor outcomes.","palabras_clave":"IL-6 RECEPTOR|SPINDLE SHAPED STROMAL CELLS|BREAST CANCER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5828,"idioma_id":4,"titulo":"Identification of Brucella ovis exclusive genes in field isolates from Argentina","resumen":"agregar resumen","palabras_clave":"BRUCELLA|ARGENTINA|EPIDEMIOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5829,"idioma_id":4,"titulo":"Rh-decorated PtIrOx nanoparticles for glycerol electrooxidation: Searching for a stable and active catalyst","resumen":"tWe report a fast method of producing rhodium-decorated platinum nanoparticles (NPs) containingiridium oxides (IrOx) to be used in the glycerol electrooxidation reaction. We synthesize PtIrOx\/C elec-trocatalysts of different atomic compositions dispersed on Carbon Vulcan XC-72R®by using the fastpolyol method assisted by microwaves. Afterwards, PtIrOx\/C was potentiodynamically decorated byRh (Rh\/PtIrOx\/C). The NPs are characterized by energy dispersive X-ray analysis, X-ray diffraction, X-ray photoelectron spectroscopy and transmission electron microscopy. The electrooxidation of glycerolwas investigated in acid medium by cyclic voltammetry and chronoamperometry. The electrochemicalstability of Rh\/PtIrOx\/C NPs was evaluated by following a degradation test protocol, which consists inexhaustive cyclic voltammetries. Our results show that the presence of iridium oxides in the architec-ture of platinum enhances the electrochemical stability of the catalyst by avoiding agglomeration effects.Moreover, the presence of rhodium catalyzes the glycerol electrooxidation reaction. These results helpunderstanding the role of Rh and IrOxin the glycerol electrooxidation and provide new insights fordesigning nanomaterials with improved stability and activity.","palabras_clave":"FUEL CELLS|IRIDIUM OXIDES|RHODIUM|GLYCEROL ELECTROOXIDATION|STABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5830,"idioma_id":4,"titulo":"Improved Sr0.6La0.4Co0.8Fe0.2O3¡d\/Ce0.8Y0.2O2¡d interface for IT-SOFC applications","resumen":"One of the remaining challenges for the solid oxide fuel cell (SOFC) technology isdecreasing its operation temperature to the 500e800 C range in a cost-effective waywithout diminishing the cell performance. In this work, we studied a symmetrical cellcomposed of Sr0.6La0.4Co0.8Fe0.2O3d (LSCFO) cathodes deposited on a Ce0.8Y0.2O2d (CYO)substrate by the simple, cost-effective and scalable spin coating technique. The nanostructuredcathode is composed of agglomerated ~100e200 nm particles with ~50 nmcrystallite size. Electrochemical impedance spectroscopy (EIS) analysis indicates thatelectrolyte grain boundary conductivity strongly depends on the cathode nature. Thedetailed high resolution transmission electron microscopy (HR-TEM) characterization ofthe LSCFO\/CYO interface reveals that the LSCFO cathode is well adhered to the CYOelectrolyte. The improved nano\/microstructure of the cathodes and the cathode\/electrolyteinterface yields to cathode area specific resistance (ASR) values as low as 0.4 Ucm2 at550 C, being these values even lower than those previously reported in literature forcathodes in optimized LSCFO\/CYO\/YSZ\/CYO\/LSCFO cells.","palabras_clave":"SOLID OXIDE FUEL CELL|ELECTROLYTE|CATHODE|INTERFACE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5832,"idioma_id":5,"titulo":"Contribución al estudio polínico de la flora de la cuenca Laguna Avendaño (Ñuble, Chile)","resumen":"Se analiza la morfología polínica de 29 especies nativas, típicas de la zona mediterránea de Chile. El objetivo de la investigación es aportar nuevos datos sobre la flora polínica de la zona central y de esta forma contribuir a la información disponible para estudios paleopalinológicos.Se trabajó con material recolectado dentro de la cuenca de la Laguna Avendaño y con algunos ejemplares provenientes del Herbario de la Universidad de Concepción. Las muestras fueron acetolizadas. Se analizaron y se tomaron fotos con microscopía óptica (MO). Las caracterizaciones polínicas amplían estudios previos realizados por diversos autores excepto en 2 de las especies consideradas, que son descriptas por primera vez, Baccharis pingraea DC. y Haplopappus integerrimus (Hook. & Arn.) H.M. Hall, (Asteraceae).","palabras_clave":"LAGUNA AVENDAÑO|POLEN|FLORA POLÍNICA|PALINOLOGÌA CHILE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5833,"idioma_id":5,"titulo":"Interpretación estructural del sector norte del flatslab pampeano a partir de datos magnéticos. Precordillera de San Juan y la Rioja","resumen":"La región andina entre los -28° a -31° incluye la transición de las provincias geológicas: Cordillera Frontal, Precordillera y Sierras Pampeanas Occidentales en las provincias de San Juan y La Rioja. La evolución geológica y un régimen tectónico compresivo del área podrían haber dejado su impronta en zonas donde micro continentes colisionaron y se amalgamaron, identificadas como zonas de sutura. A partir de una base de datos magnéticos compilados desde diferentes tipos de fuentes (aéreas y terrestres) y adquiridos en diferentes épocas, se aplicó una serie de reducciones geofísicas con el propósito de obtener una única base de datos homogénea. A la carta de anomalías magnéticas Totales, se le aplicaron dos métodos de filtrado diferentes: Continuación Analítica Ascendente y Frecuencial. Finalmente, sobre perfiles transversales en dirección Este ? Oeste, se calcularon soluciones a partir de la Deconvolución de Werner 2D. Hemos interpretado la disposición de unidades con diferente susceptibilidad magnética correlacionando estas con la configuración de terranes paleozoicos amalgamados en esta región. La ubicación de las soluciones de Werner nos permitió reconocer el comportamiento en profundidad de las estructuras aflorantes.","palabras_clave":"Métodos Potenciales|Anomalías|Filtros Pasabandas|Residuales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5834,"idioma_id":5,"titulo":"Explicar y contrastar","resumen":"Usualmente se ha asumido que una única distinción puede dar cuenta del rol que cumplen los conceptos en una teoría respecto de la contrastación y respecto de la explicación. Intentaremos mostrar que esta asunción es incorrecta. Por una parte, no hay razones para considerar que esta coincidencia deba darse, y por otra, como se intentará mostrar a partir de varios ejemplos, de hecho, no se da. La base de contrastación de una teoría no tiene por qué coincidir con el explanandum de la teoría. Para defender este punto se asumirá el estructuralismo metateórico. Se extraerán consecuencias para la concepción metateórica presupuesta.","palabras_clave":"EXPLICACIÓN|CONTRASTACIÓN|T-TEORICIDAD|ESTRUCTURALISMO METATEÓRICO|DISTINCIÓN TEÓRICO OBSERVACIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5835,"idioma_id":4,"titulo":"ON THE EXISTENCE OF NON-MICROBIOTHERIAN AUSTRALIDELPHIAN MARSUPIALS (DIPROTODONTIA) IN THE EOCENE OF PATAGONIA","resumen":"A diverse assemblage of extinct mammals of early?middle Eocene age (Ypresian?Lutetian boundary) come from the Patagonian localities of La Barda and Laguna Fría around Paso del Sapo in northwestern Chubut Province (Argentina). Metatherians are well represented, mostly by dental remains of ?Didelphimorphia?, Paucituberculata, Sparassodonta, Microbiotheria, and Polydolopimorphia. Here we analyse three calcanea and one astragalus referable to the same, indeterminate taxon, from La Barda, showing the fusion of their ectal and sustentacular facets. This facet arrangement characterizes the Australidelphia, a marsupial clade represented by Microbiotheria from South America and Antarctica, plus all Australasian lineages. Other australidelphian features shown by these tarsals include: in the calcanea, a reduced peroneal process and a tripartite cuboid facet; in the astragalus, a round, very small astragalar head in relation to the body; a convex trochlea with a half for the tibia and half, in slightly lower position, for the fibula. Their size and other anatomical features suggest that the new tarsals cannot be referred to the Microbiotheria. Phylogenetic analysis suggests that the La Barda taxon lies within the Australidelphia, and that it is either closely related or belongs to, the Diprotodontia. The existence of advanced australidelphians in Patagonia adds further evidence of the close relationship between Patagonia, Antarctica, and Australia during the Late Cretaceous ? early Palaeogene.","palabras_clave":"Australidelphia|South America|Paso del Sapo Fauna|Palaeogene|Tarsal bones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5836,"idioma_id":4,"titulo":"A new genus of Sigmodontinae (Mammalia, Rodentia, Cricetidae) from the Pliocene of Central Argentina","resumen":"Chukimys favaloroi, new genus and species, is added to the past diversity of sigmodontine rodents, the largest subfamily of Neotropical mammals. It is based on a maxillary with the entire toothrow and both first and second lower molars collected in sediments of the Brochero Formation (Late Pliocene) that crop out in Córdoba province, Argentina. The new genus represents a small rodent (about 40 g) and has a unique combination of traits including a zygomatic plate with deeply excavated anterior border and conspicuous zygomatic spine, incisive foramen reaching the level of the protocone of the first upper molar, palate narrow and flat, molars moderately hypsodont and the first lower molar four-rooted. The new sigmodontine shows similarities with several extant members of the tribes Phyllotini and Reithrodontini, as well as with the extinct Dankomys vorohuensis from the Pliocene of Buenos Aires, Argentina. The comparisons performed suggest that Chukimys has anatomical features related to omnivory and the incorporation of hard material in its diet. Chukimys is the first fossil genus of sigmodontine described from Central Argentina and probably represents a new member of the currently monotypic tribe Reithrodontini.","palabras_clave":"Pliocene|Brochero Formation|Dankomys|Reithrodontini"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5837,"idioma_id":4,"titulo":"Compounds released by disturbed adults of the haematophagous bug Triatoma infestans (Hemiptera: Reduviidae): behavioural effects of single compounds and binary mixtures","resumen":"Adults of the Chagas disease vector Triatoma infestans Klug (Hemiptera: Reduviidae: Triatominae), possess paired exocrine glands: the metasternal and Brindley?s glands. Both glands are discharged by disturbed adults, releasing an alarm pheromone that elicits an escape response of larvae. The present study analyzes the individual (or combined) effects of some of the volatiles of the whole pheromone blend released under disturbance, searching for active compounds and for possible interactions (e.g. synergism, additive effects) between them. Using an experimental arena, different doses of components emitted by disturbed adults are tested against larvae. Larvae show escape responses to some of the acids, as well as to one alcohol, but no response to ketones. This is observed with certain doses of compounds from Brindley?s glands (isobutyric, butyric and acetic acid; 2-methyl-1-butanol), although the compounds tested in the present study that are produced by metasternal glands are shown to evoke random responses, suggesting that mainly Brindley?s glands are involved in the alarm context.Two combinations of two individually repellent compounds (2-methyl-1-butanol with acetic or isobutyric acid) evoke escape responses, although other combinations make the individual effect disappear. A different mixture of two individually repellent compounds (butyric and isobutyric acid) evokes attraction, although these are also host odours. The potential use of the active compounds released by disturbed adults to monitor triatominepopulations is discussed.","palabras_clave":"ALARM PHEROMONE|BRINDLEY'S GLANDS|VOLATILES|CHAGAS DISEASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5838,"idioma_id":4,"titulo":"Artificial perches promote vegetation restoration","resumen":"Native ecosystems are continuously beingtransformed mostly into agricultural lands. Simultaneously, a large proportion of fields are abandonedafter some years of use. Without any intervention,altered landscapes usually show a slow reversion tonative ecosystems, or to novel ecosystems. One of themain barriers to vegetation regeneration is poorpropagule supply. Many restoration programs havealready implemented the use of artificial perches inorder to increase seed availability in open areas wherebird dispersal is limited by the lack of trees. Toevaluate the effectiveness of this practice, we performed a series of meta-analyses comparing the use ofartificial perches versus control sites without perches.We found that setting-up artificial perches increasesthe abundance and richness of seeds that arrive inaltered areas surrounding native ecosystems. Moreover, density of seedlings is also higher in open areaswith artificial perches than in control sites withoutperches. Taken together, our results support the use ofartificial perches to overcome the problem of poorseed availability in degraded fields, promoting and\/oraccelerating the restoration of vegetation in concordance with the surrounding landscape.","palabras_clave":"SEED DISPERSAL|SEEDLING-ESTABLISHMENT|FRUIT-EATING BIRDS|DEGRADED LANDSCAPES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5839,"idioma_id":4,"titulo":"Chiral analysis of derivatized amino acids from kefir by gas chromatography","resumen":"Theamino acids presentin the kefir sampleswere derivatized using different alkyl chloroformates combined withalcohols and chromatographed on a(3-O-butanoyl-2,6-di-O-pentyl)-&#947;-cyclodextrin(Lipodex E) chiral gas-liquid chromatographic column. The combinationof derivatization reagents that led to  better chromatographicperformance for standard amino acid solutions was criticallycompared. All the chloroformate\/alchohol mixtures lead to fast andquantitative reactions.However,theethyl chloroformate\/ethanolmixture was selected sincethe number of enantioseparated amino acid derivatives was higher.The derivatization was applied to samples of kefir grains grown in raw and in skimmedUHT milk. The samples were previously pretreated byelution froma lowpressure ion-exchange columns. Standard addition calibration curveshave been usedfor the quantitative determinations of aminoacid derivatives in both samples, and the figures of merit of theproposed method have been assessed. TheL- and D- configuration of D- and L-alanine, D- and L-valine,D-proline, L-threonine, aspartic and glutamic acids, methionine andcysteine were detected and quantitatively determined.  ","palabras_clave":"Gas Chromatography|Chiral|Amino acids analysis|Kefir"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5840,"idioma_id":4,"titulo":"Analysis of citrulline and metabolic related amino acids in plasma by derivatization and RPLC. \tApplication of the extrapolative  internal standard calibration method","resumen":"We propose to apply the extrapolative internal standard calibration method (EISM) for quantitative analysis of citrulline and other metabolically related amino acids in plasma by pre-columnderivatization, liquid chromatography and UV detection. Whenever derivatization is required and an amino acid-free matrix is nonexistent, the analytical challenge is to detect and correct matrix effects and, also to control the experimental conditions of the chemical reaction, mainly the concentration of the derivatizing reagents.Plasma samples contain free amino acids, non-peptidic amino acids and other amino compounds, such as biogenic amines, that would react with most derivatizing reagents and interfere in the analysis. In this particular work, and considering the scope of the intended application, chromatographic conditions were chosen for the analysis of citrulline, arginine, glutamine, glutamic acid, proline and ornithine by precolumn reaction with FMOC-Cl andRP-HPLC-UV analysis.Linear least square regression of analyte to internal standard (IS) signal ratio versus the amount of spiked standard amino acidwere established and linear regression parameters for each curvewere calculated. The information provides by an IS, which is also derivatized, can be used to detect matrix effects due to the chemical reaction. This calibration method was validated and the figures of merit were estimated.","palabras_clave":"Extrapolative internal Standard method|citrulline|RP-HPLC|Precolumn derivatization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5841,"idioma_id":4,"titulo":"Chiral ligand-exchange chromatography with Cinchona alkaloids. Exploring experimental \tconditions for enantioseparation of &#945;-amino acids","resumen":"The natural Cinchona alkaloid quinidine as chiral selector in chiral ligand-exchange chromatography was systematicallystudied. Chromatographic conditions for enantioseparation of twenty&#945;-amino acidswere first timestudiedby changing mobile phase parameters such as pH, concentration of organic solvent, type of salt, ligand tometal ratio and also column temperature. Maximum retention and enantioselectivity factors were observed atthe region close to pH = 8, since the tertiary amine (the quinuclidinic nitrogen) of the quinidine is protonatedonly in a small degree, and therefore is available for the chelate formation. Additionally at this pH value thereis no other competing ligand for complex the metallic cation. The thermodynamic transfer parameters of theenantiomers from the mobile to the stationary phase from van´t Hoff plots within the range of 10?35 °C wereestimated. Thus, the differences in the transfer enthalpy &#916;(&#916;H), and transfer entropy &#916;(&#916;S) enabled an investigationof the origin of the differences in interaction energies &#916;(&#916;G). Finally, the molecular geometry of theformed diastereomeric complexes was modelled and energetic differences between both compounds were calculatedby a semi empirical method.","palabras_clave":"Chiral ligand-exchange|Cinchona alkaloids|a-Amino acids|Molecular geometry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5842,"idioma_id":4,"titulo":"?Enantioselective gas chromatography with functionalized cyclodextrins as chiral selectors. \tFundamentals of the measurement of absolute association constants using capillary columns","resumen":"Chiral capillary GC columns containing different amounts of octakis(6-O-tertbutyldimethylsilyl-2,3-di-O-acetil)-&#947;-cyclodextrin as chiral selector dissolved in a polymeric matrix were constructed with the aim of determining enantiomeric association constants between a group of well resolved chiral N-trifluoroacetyl amino acid methyl esters and this specific selector at different temperatures. The most relevant sources of uncertainties in the experimental data (hold-up and retention times, and column phase ratios at each temperature) were assessed.These cyclodextrin-based columns are known to enantioseparate a wide variety of chemicalcompounds, thus, the measurement of the absolute enantioselective constants of a group ofsolutes with this selector can be useful for systematic studies aimed to a general understanding about how these selectors work. These absolute association constants were estimated from data collected from very simplified experimental systems, and by using the fundamental gas-liquid chromatography equations.","palabras_clave":"Chiral gas-liquid chromatography|Capillary columns|Association constants|Octakis(6-O-tertbutyldimethylsilyl- 2,3-di-O-acetil)-&#947;-cyclodextrin|N-trifluoroacetyl amino acid methyl esters"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5843,"idioma_id":4,"titulo":"A deregulated expression of estrogen-target genes is associated with an altered response to estradiol in aged rats perinatally exposed to bisphenol A","resumen":"Here weassessed the effects of perinatal exposure to bisphenol A (BPA) on the uterine response to17&#946;-estradiol (E2) in aged rats. Pregnant rats were orally exposed to 0.5 or 50&#956;g BPA\/kg\/day from gestational day 9 until weaning. Onpostnatal day (PND) 360, the rats were ovariectomized and treated with E2 forthree months. Theuterine tissue of BPA50 and BPA0.5 rats showed increased density of glands with squamous metaplasia (GSM) and glands withdaughter glands respectively. Wnt7a expression waslower in GSM of BPA50 rats than in controls. The expression of estrogenreceptor 1 (ESR1) and its 5´- untranslated exons ESR1-O and ESR1-OT was lower in BPA50 rats. Both doses of BPAmodified the expression of coactivator proteins and epigenetic regulatoryenzymes. Thus, perinatal BPA-exposed ratsshowed different glandular abnormalities associatedwith deregulated expression of E2-target genes. Different mechanisms would be involved depending on theBPA dose administered. ","palabras_clave":"Bisphenol A |ESR1|Uterine glands|Wnt"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5844,"idioma_id":4,"titulo":"Molecular mechanisms underlying pituitary pathogenesis","resumen":"During the last years, progress has been made on the identification of mechanisms involved in anterior pituitary cell transformation and tumorigenesis. Oncogene activation, tumor suppressor gene inactivation, epigenetic changes, and microRNAs deregulation contribute to the initiation of pituitary tumors. Despite the high prevalence of pituitary adenomas, they are mostly benign, indicating that intrinsic mechanisms may regulate pituitary cell expansion. Senescence is characterized by an irreversible cell cycle arrest and represents an important protective mechanism against malignancy. Pituitary tumor transforming gene (PTTG) is an oncogene involved in early stages of pituitary tumor development, and also triggers a senescence response by activating DNA-damage signaling pathway. Cytokines, as well as many other factors, play an important role in pituitary physiology, affecting not only cell proliferation but also hormone secretion. Special interest is focused on interleukin-6 (IL-6) because its dual function of stimulating pituitary tumor cell growth but inhibiting normal pituitary cells proliferation. It has been demonstrated that IL-6 has a key role in promoting and maintenance of the senescence program in tumors. Senescence, triggered by PTTG activation and mediated by IL-6, may be a mechanism for explaining the benign nature of pituitary tumors.","palabras_clave":"IL-6|OIS|PTTG|Pituitary tumors|Senescence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5845,"idioma_id":4,"titulo":"Tectonic insight based on anisotropy of magnetic susceptibility and compaction studies in the Sierras Australes thrust and fold belt (southwest Gondwana boundary, Argentina)","resumen":"Abstract The Sierras Australes fold and thrust belt (Buenos Aires Province, Argentina) was in the southwesternGondwanaland margin during the Paleozoic. The Tunas Formation (Permian) is exposed along the easternpart of it and continues eastward beneath the Claromecó Basin. Anisotropy of magnetic susceptibility (AMS)and compaction studies are described and compared with previous paleomagnetic studies with the aim ofdetermining direction and magnitude of the main stresses acting during the sedimentation of the TunasFormation. The anisotropy ellipsoids are triaxial with oblate or prolate shapes, reflecting different stages of layerparallel shortening during the evolution of the basin. Kmax axes trend NW-SE, parallel to the fold axes, while Kminmove from a horizontal (base) to a vertical orientation at the top of the succession, showing a change froma tectonic to almost a sedimentary fabric. The magnitude of anisotropy and compaction degree decreasestoward the top of the succession. The AMS results are consistent with the outcrop structural observations andthe compaction and paleomagnetic data. Regional pattern indicates a compression from the SWalong this partof Gondwana, with a migration of the orogenic front and attenuation toward the NE in the foreland basinduring the Upper Paleozoic. This deformation, locally assigned to the San Rafael noncollisional orogenic phase,is the result of the latitudinal movements toward the Equator of Gondwana (southern plates) and Laurentia(northern plates) during the Permian. This movement is the result of a rearrangement of the microplates thatcollided with Gondwana during the Late Devonian, to configure Pangea during the Triassic.","palabras_clave":"Paleogeography|Tectonic|Gondwana|Pangea"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5846,"idioma_id":4,"titulo":"Systematics and evolutionary significance of the small Abrocomidae from the early Miocene of southern South America","resumen":"Octodontoidea is the most species-rich clade among hystricomorph rodents, and has a fossil record going back to at least the late Oligocene. Affinities of fossils previous to the late Miocene differentiation of the extant families Abrocomidae, Echimyidae and Octodontidae are controversial, essentially because these fossils may share few apomorphies with modern species. In fact, pre-late Miocene representatives of Abrocomidae had not been recognised until very recently. Here we revise the early Miocene genus Acarechimys, originally assigned to Echimyidae, and alternatively to stem Octodontoidea or to Octodontidae. A systematic and parsimony-based phylogenetic analysis of the species traditionally included in Acarechimys showed that this genus is part of stem Abrocomidae. These results are primarily supported by morphology of the mandible and lower molars. Acarechimys is here restricted to three species, A. minutus, A. pulchellus and Acarechimys pascuali sp. nov., while another species, A. constans, is here transferred to a new abrocomid genus. The remaining species were nested within Octodontidae. According to these results, Abrocomidae might have been as diverse as its sister clade Octodontidae-Echimyidae during the late Oligocene?early Miocene. Extinction of this diversity would have resulted in marked loss of evolutionary history, with extant abrocomids being currently restricted to late-diverged euhypsodont representatives.","palabras_clave":"Rodentia|Octodontoidea|early Miocene|South America|phylogeny"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5847,"idioma_id":4,"titulo":"Indicators of landscape organization and functionality in semi-arid former agricultural lands under a passive restoration management over two periods of abandonment","resumen":"Abandoned lands previously used for agricultural purposes may constitute an opportunity for under-standing the variables involved in the restoration of native ecosystems over time. In this study, weassessed the functional status of an abandoned farmland currently used for conservation, using a method-ology based on indicators of landscape organization and soil surface. We analyzed changes in plant cover,patch and interpatches structure and several soil surface properties during two periods of land aban-donment (less and more than 40 years). Using this methodology, we characterize the potential of theecosystem to capture and transfer resources, and the state of functional properties such as infiltrationcapacity, soil stability and nutrient cycling.We detected a significant development of the shrub layer and an increase of the number, type andarea occupied by vegetated patches in old fields. The contribution of vegetated patches to the recovery ofstability, infiltration and nutrient functions was significant at the local scale. Nonetheless, when analyzingthe landscape as a whole only the nutrient cycling index was significantly higher in old fields. The lackof improvement of the stability and infiltration in old fields can lead to further degradation and indicatesthat even though vegetation cover may have improved over time in old fields, the current cover mightnot be enough to prevent further degradation by erosion. Our results reinforce the importance of usingfunctionality indexes in future studies focused on adequate restoration measures to protect the functionof desert ecosystems, especially when aimed for biodiversity conservation. Hence, the use of monitoringsystems based on organization and function indicators is a useful tool to represent the current state andpotential recovery of previously disturbed ecosystems, provided that the time scale is taken into account","palabras_clave":"LAND ABANDONMENT|PASSIVE MANAGEMENT|DEGRADATION|MONTE DESERT|LANDSCAPE FUNCTION ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5848,"idioma_id":4,"titulo":"Combined local and systemic bleomycin administration in electrochemotherapy for reducing the number of treatment sessions","resumen":"Background. Electrochemotherapy (ECT), a medical treatment widely used in human patients for tumor treatment,increases bleomycin toxicity by 1000 fold in the treated area with an objective response rate of around 80%. Despiteits high response rate, there are still 20% of cases in which the patients are not responding. This could be ascribed tothe fact that bleomycin, when administered systemically, is not reaching the whole tumor mass properly because ofthe characteristics of tumor vascularization, in which case local administration could cover areas that are unreachableby systemic administration.Patients and methods. We propose combined bleomycin administration, both systemic and local, using companionanimals as models. We selected 22 canine patients which failed to achieve a complete response after an ECTtreatment session. Eleven underwent another standard ECT session (control group), while 11 received a combinedlocal and systemic administration of bleomycin in the second treatment session.Results. According to the WHO criteria, the response rates in the combined administration group were: completeresponse (CR) 54% (6), partial response (PR) 36% (4), stable disease (SD) 10% (1). In the control group, these were:CR 0% (0), PR 19% (2), SD 63% (7), progressive disease (PD) 18% (2). In the combined group 91% objective responses(CR+PR) were obtained. In the control group 19% objective responses were obtained. The difference in the responserate between the treatment groups was significant (p < 0.01).Conclusions. Combined local and systemic bleomycin administration was effective in previously to ECT non respondingcanine patients. The results indicate that this approach could be useful and effective in specific populationof patients and reduce the number of treatment sessions needed to obtain an objective response.","palabras_clave":"electrochemotherapy|combined treatment|resistant|systemic and local"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5849,"idioma_id":4,"titulo":"Ultra high speed deterministic algorithm for transmission lines disturbance identification based on principal component analysis and Euclidean norm","resumen":"Protection devices are designed to provide high sensitivity to transients produced by undesirable conditions like lightning stroke, avoiding their operation under all tolerable events like switching operations. The problem of incorrect operation due to transient phenomena can be handled by two means, one is to allow the transients and provide additional logics in the relay, other means is to damp the oscillation from source side. Protection relays´ not always must trip or send a trip signal and sometimes, only an alarm is necessary. In this context, this research presents a fast and reliable formulation for transmission lines (TLs) switching operations and lightning strokes detection and identification. The proposed methodology is based on Principal Component Analysis (PCA) and Euclidean Norm (EN); by using PCA it is possible to determine that normal operation signals describe a very well defined Ellipsoidal Pattern (EP). In this manner, by calculating the Euclidean Norm (EN) among Principal Components (PCs) for each sample and the origin of the reference Ellipsoidal Pattern, switching operations and lightning strokes are detected and identified. Test results show that the proposed algorithm presents high success on phenomena detection and identification, presenting a high potential for online applications.","palabras_clave":"Deterministic algorithm|Ellipsoidal pattern|Euclidean norm|Undesirable conditions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5850,"idioma_id":4,"titulo":"Physicochemical properties of sorghum and technological aptitude for popping. Nutritional changes after popping","resumen":"The aims were to characterize 28 hybrids of red (RS) and white (WS) sorghum by physicochemical analysis, relate themwith their technological aptitude for popping, and asses nutritional changes after popping in selected samples. Throughprincipal component analysis it was observed that the higher the grain hardness, the higher apparent volume of poppedproduct was, and performance was higher for WS. An indicator of the ability of sorghum hybrids to pop was developed.Comparing precooked samples with their native flours, no significant difference for protein and fat content was observed,but ash, dietary fiber, and minerals were reduced by popping. Protein digestibility increased but available lysine decreased(WS:1.3 and RS:1.2 times). Phytic acid, polyphenols and antioxidant capacity were reduced (WS: 1.3 and RS: 1.5; WS:1.3 and RS: 1.8; WS: 1.3 and RS: 1.7 times, respectively), but whole flour precooked by popping can be considered animportant source of antioxidants. Popped sorghum is an interesting alternative as food itself, or as ingredient for makingother foods such as cereal bars.","palabras_clave":"WHOLE GRAIN|SORGHUM|POPPING|ANTIOXIDANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5851,"idioma_id":5,"titulo":"Aplicación de Redes Neuronales Artificiales: Predicción de rendimientos del proceso de extracción de aceite de canola","resumen":"Las Redes Neuronales Artificiales (RNA) son una herramienta de inteligencia artificial utilizada en amplios campos del conocimiento. Una de sus aplicaciones estudiadas en la actualidad es la capacidad de modelar y realizar predicciones en sistemas complejos. En este trabajo se utilizó una RNA  feedforward  perceptron  multicapa para la predicción de rendimientos de extracción de aceite de canola considerando 3 variables de entrada: tiempo de extracción, temperatura y contenido total de aceite del grano, los cuales se contrastaron con datos experimentales. El entrenamiento y ajuste de la red se realizó con datos experimentales de granos de canola de distintos contenidos de aceite total al seleccionado para la predicción. Generalmente para los rendimientos simulados se obtuvieron diferencias con respecto a los datos experimentales de 2 % b.s. y dentro del rango del error de medición experimental. Estos resultados muestran la eficacia y potencialidad del uso de las redes neuronales para  la predicción del comportamiento de un proceso de extracción de aceite a partir de parámetros característicos de la materia prima y el proceso.","palabras_clave":"REDES NEURONALES ARTIFICIALES|PREDICCION|EXTRACCION DE ACEITE|CANOLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5852,"idioma_id":4,"titulo":"Implications of gamma band activity in the pedunculopontine nucleus.","resumen":"The fact that the pedunculopontine nucleus (PPN) is part of the reticular activating system places it in a unique position to modulate sensory input and fight-or-flight responses. Arousing stimuli simultaneously activate ascending projections of the PPN to the intralaminar thalamus to trigger cortical high-frequency activity and arousal, as well as descending projections to reticulospinal systems to alter posture and locomotion. As such, the PPN has become a target for deep brain stimulation for the treatment of Parkinson´s disease, modulating gait, posture, and higher functions. This article describes the latest discoveries on PPN physiology and the role of the PPN in a number of disorders. It has now been determined that high-frequency activity during waking and REM sleep is controlled by two different intracellular pathways and two calcium channels in PPN cells. Moreover, there are three different PPN cell types that have one or both calcium channels and may be active during waking only, REM sleep only, or both. Based on the new discoveries, novel mechanisms are proposed for insomnia as a waking disorder. In addition, neuronal calcium sensor protein-1 (NCS-1), which is over expressed in schizophrenia and bipolar disorder, may be responsible for the dysregulation in gamma band activity in at least some patients with these diseases. Recent results suggest that NCS-1 modulates PPN gamma band activity and that lithium acts to reduce the effects of over expressed NCS-1, accounting for its effectiveness in bipolar disorder.","palabras_clave":"AROUSAL|GAMMA BAND OSCILLATIONS|P\/Q-TYPE CHANNELS|N-TYPE CHANNELS|SLEEP\/WAKE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5853,"idioma_id":4,"titulo":"Cognitive enhancers versus addictive psychostimulants: The good and bad side of dopamine on prefrontal cortical circuits","resumen":"In this review we describe how highly addictive psychostimulants such as cocaine and methamphetamineactions might underlie hypoexcitabilty in frontal cortical areas observed in clinical and preclinical modelsof psychostimulant abuse. We discuss new mechanisms that describe how increments on synapticdopamine release are linked to reduce calcium influx in both pre and postsynaptic compartments onmedial PFC networks, therefore modulating synaptic integration and information. Sustained DA neuromodulationby addictive psychostimulants can ?lock? frontal cortical networks in deficient states. On theother hand, other psychostimulants such as modafinil and methylphenidate are considered pharmacologicalneuroenhancement agents that are popular among healthy people seeking neuroenhancement.More clinical and preclinical research is needed to further clarify mechanisms of actions and physiologicaleffects of cognitive enhancers which show an opposite pattern compared to chronic effect of addictivepsychostimulants: they appear to increase cortical excitability. In conclusion, studies summarizedhere suggest that there is frontal cortex hypoactivity and deficient inhibitory control in drug-addictedindividuals. Thus, additional research on physiological effects of cognitive enhancers like modafinil andmethylphenidate seems necessary in order to expand current knowledge on mechanisms behind theirtherapeutic role in the treatment of addiction and other neuropsychiatric disorders.","palabras_clave":"DOPAMINE|MODAFINIL|METHYLPHENIDATE|METHAMPHETAMINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5854,"idioma_id":4,"titulo":"Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum","resumen":"Caffeine is the world's most popular psychoactive drug and is also an active adulterant found in many drugs of abuse, including seized cocaine samples. Despite several studies which examine the effects of caffeine or cocaine administered as single agents, little data are available for these agents when given in combination. The purpose of the present study was to determine if combined intake of both psychostimulants can lead to maladaptive changes in striatal function. Mice were injected with a binge regimen (intermittent treatment for 13 days) of caffeine (3 × 5 mg\/kg), cocaine (3 × 10 mg\/kg), or combined administration. We found that chronic caffeine potentiated locomotion induced by cocaine and that both caffeine-treated groups showed sensitization. Striatal tissue was obtained 24 h and 7 days after last injection (withdrawal) for immunohistochemistry and mRNA expression. Our results show that combined intake of both psychostimulants can increase GFAP immunoreactivity in the striatum at both times post treatment. Gene expression analysis, targeted at dopamine, adenosine, and glutamate receptor subunit genes, revealed significant transcript down-regulation in the dorsal striatum of AMPA, NMDA, D1 and D2 receptor subunit mRNA expression in the group that received combined treatment, but not after individual administration. At withdrawal, we found increased D1 receptor mRNA expression along with increased A1, AMPA, NMDA, and metabotropic subunit expression. A2A mRNA showed decreased expression after both times in all experimental groups. Our study provides evidence that there are striatal alterations mediated by combined caffeine and cocaine administration, and highlights negative outcomes of chronic intake of both psychostimulants.","palabras_clave":"COCAINE|CAFFEINE|STRIATUM|LOCOMOTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5855,"idioma_id":4,"titulo":"Seed dormancy responses to temperature relate to Nothofagus species distribution and determine temporal patterns of germination across altitudes in Patagonia","resumen":"Seeds integrate environmental cues that modulate their dormancy and germination.Although many mechanisms have been identified in laboratory experiments, their contributionto germination dynamics in existing communities and their involvement in definingspecies habitats remain elusive. By coupling mathematical models with ecological data we investigated the contribution ofseed temperature responses to the dynamics of germination of three Nothofagus species thatare sharply distributed across different altitudes in the Patagonian Andes. Seed responsiveness to temperature of the three Nothofagus species was linked to the thermalcharacteristics of their preferred ecological niche. In their natural distribution range, therewas overlap in the timing of germination of the species, which was restricted to mid-spring.By contrast, outside their species distribution range, germination was temporally uncoupledwith altitude. This phenomenon was described mathematically by the interplay between interspecificdifferences in seed population thermal parameters and the range in soil thermic environmentsacross different altitudes. The observed interspecific variations in seed responsiveness to temperature and its environmentalregulation, constitute a major determinant of the dynamics of Nothofagus germinationacross elevations. This phenomenon likely contributes to the maintenance of patterns ofspecies abundance across altitude by placing germinated seeds in a favorable environment forplant growth.","palabras_clave":"ALTITUDINAL GRADIENT|ECOLOGICAL NICHE|FOREST REGENERATION|GERMINATION|NOTHOFAGUS|TEMPERATURE|THERMAL TIME"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5856,"idioma_id":4,"titulo":"Comparative genomics provides new insights into the diversity,  physiology, and sexuality of the only industrially exploited  tremellomycete: Phaffia rhodozyma","resumen":"                Background                The class Tremellomycete (Agaricomycotina) encompasses more than 380 fungi. Although there are a few edible Tremella spp., the only species with current biotechnological use is the astaxanthin-producing yeast Phaffia rhodozyma (Cystofilobasidiales). Besides astaxanthin, a carotenoid pigment with potent antioxidant activity and great value for aquaculture and pharmaceutical industries, P. rhodozyma possesses multiple exceptional traits of fundamental and applied interest. The aim of this study was to obtain, and analyze two new genome sequences of representative strains from the northern (CBS 7918T, the type strain) and southern hemispheres (CRUB 1149) and compare them to a previously published genome sequence (strain CBS 6938). Photoprotection and antioxidant related genes, as well as genes involved in sexual reproduction were analyzed.                              Results                Both genomes had ca. 19&nbsp;Mb and 6000 protein coding genes, similar to CBS 6938. Compared to other fungal genomes P. rhodozyma strains and other Cystofilobasidiales have the highest number of intron-containing genes and highest number of introns per gene. The Patagonian strain showed 4.4&nbsp;% of nucleotide sequence divergence compared to the European strains which differed from each other by only 0.073&nbsp;%. All known genes related to the synthesis of astaxanthin were annotated. A hitherto unknown gene cluster potentially responsible for photoprotection (mycosporines) was found in the newly sequenced P. rhodozyma strains but was absent in the non-mycosporinogenic strain CBS 6938. A broad battery of enzymes that act as scavengers of free radical oxygen species were detected, including catalases and superoxide dismutases (SODs). Additionally, genes involved in sexual reproduction were found and annotated.                              Conclusions                A draft genome sequence of the type strain of P. rhodozyma is now available, and comparison with that of the Patagonian population suggests the latter deserves to be assigned to a distinct variety. An unexpected genetic trait regarding high occurrence of introns in P. rhodozyma and other Cystofilobasidiales was revealed. New genomic insights into fungal homothallism were also provided. The genetic basis of several additional photoprotective and antioxidant strategies were described, indicating that P. rhodozyma is one of the fungi most well-equipped to cope with environmental oxidative stress, a factor that has probably contributed to shaping its genome.              ","palabras_clave":"GENOMICS|ASTAXANTHIN|SUNSCREENS|XANTHOPHYLLOMYCES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5857,"idioma_id":5,"titulo":"Morfometría y topología del cerebro del pez tetra cola roja, Aphyocharax anisitsi (Characiformes: Characidae)","resumen":"En este trabajo consistió en realizar una descripción histológica de los núcleos neuronales presentes en las distintas regiones del cerebro del pez tetra cola roja, Aphyocharax anisitsi. Asimismo, se estudió la morfología externa del cerebro, cuantificando la longitud y el área de los lóbulos principales (bulbos olfatorios, hemisferios telencefálicos, lóbulos ópticos y cerebelo). Se realizó un ANOVA para determinar si existían diferencias significativas entre dichos lóbulos. Se fijaron diez animales en solución de Bouin. Los cerebros fueron disecados, se realizó la descripción anatómica externa y la captura digital de imágenes. Para el estudio de la topología interna, se siguió un protocolo histológico para parafina, se realizaron cortes en micrótomo y coloración de Nissl. Los resultadosmostraron la presencia de los lóbulos típicos descriptos para otras especies de teleósteos. Se encontraron diferencias significativas en el análisis morfométrico: los lóbulos ópticos presentaron mayor área y longitud, mientras que los bulbos olfatorios el menor tamaño. Estos resultados sugieren un predominio del sistema visual. El cerebelo presentó una mayor longitud total con respecto a los hemisferios telencéfalicos,; no se encontraron diferencias en el área. En relación a la topología interna, se observaron los núcleos asociados al cerebro anterior, medio y posterior. Los núcleos hallados en las distintas regiones no presentaron diferencias con respecto a aquellos descriptos en otros miembros del superorden Ostariophysi, como el pez cebra (Danio rerio) o el neón cardenal (Paracheirodon axelrodi).","palabras_clave":"CEREBRO|CARACIFORMES|HIPOTALAMO|TETRA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5858,"idioma_id":4,"titulo":"Lack of functional link in the tadpole morphology induced by predators","resumen":"Most studies of predator-induced plasticity have focused on documenting how prey species respondto predators by modifying phenotypic traits and how traits correlate with fitness. We havepreviously shown that Pleurodema thaul tadpoles exposed to the dragonfly Rhionaeschna variegataresponded strongly by showing morphological changes, less activity, and better survival thannon-exposed tadpoles. Here, we tested whether there is a functional link between morphologicalplasticity and increased survival in the presence of predators. Tadpoles that experienced predationrisk were smaller, less developed, and much less active than tadpoles without this experience.Burst speed did not correlate significantly with morphological changes and predator-induceddeeper tails did not act as a lure to divert predator strikes away from the head. Although we havepreviously found that tadpoles with predator-induced morphology survive better under a directpredator threat, our results on the functional link between morphology and fitness are not conclusive.Our results suggest that in P. thaul tadpoles (1) burst speed is not important to evade predators,(2) those exposed to predators reduce their activity, and (3) morphological changes do notdivert predator attacks away from areas that compromise tadpole survival. Our results showthat morphological changes in P. thaul tadpoles do not explain burst speed or lure attraction, althoughthere was a clear reduction of activity, which itself reduces predation. We propose thatchanges in tadpole activity could be further analyzed from another perspective, with morphologicalchange as an indirect product of behavior mediated by physiological mechanisms.","palabras_clave":"INDUCED MORPHOLOGY|LURE EFFECT|PREDATOR EXPOSURE|SWIMMING PERFORMANCE|TADPOLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5859,"idioma_id":4,"titulo":"Time-varying inductance of the plasma sheet in the PF1000 Plasma-Focus device","resumen":"The time varying inductance of the system plasma-electrodes in a large Plasma Focus device (PF1000) is experimentally determined from voltage and current-time derivative signals for several shots performed at the same conditions (2.4 mbar D2, 24 kV), and using these results, the voltage drop on the pinch column is also evaluated as a function of the time. The maximum values of these voltage drops exceed 100 kV in all the shots.","palabras_clave":"plasma focus|self-compression|PF-1000"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5860,"idioma_id":4,"titulo":"Ancient mitochondrial DNA reveals convergent evolution of giant short-faced bears (Tremarctinae) in North and South America","resumen":"The Tremarctinae are a subfamily of bears endemic to the New World,including two of the largest terrestrial mammalian carnivores that haveever lived: the giant, short-faced bears Arctodus simus from North Americaand Arctotherium angustidens from South America (greater than or equal to1000 kg). Arctotherium angustidens became extinct during the EarlyPleistocene, whereas Arctodus simus went extinct at the very end of thePleistocene. The only living tremarctine is the spectacled bear (Tremarctosornatus), a largely herbivorous bear that is today only found in SouthAmerica. The relationships among the spectacled bears (Tremarctos), SouthAmerican short-faced bears (Arctotherium) and North American shortfacedbears (Arctodus) remain uncertain. In this study, we sequenced amitochondrial genome from an Arctotherium femur preserved in a Chileancave. Our molecular phylogenetic analyses revealed that the SouthAmerican short-faced bears were more closely related to the extant SouthAmerican spectacled bear than to the North American short-faced bears.This result suggests striking convergent evolution of giant forms in thetwo groups of short-faced bears (Arctodus and Arctotherium), potentially asan adaptation to dominate competition for megafaunal carcasses.","palabras_clave":"Carnivora|Phylogeny|Ancient DNA|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5861,"idioma_id":4,"titulo":"Turning-Off Signaling by Siglecs, Selectins, and Galectins: Chemical inhibition of Glycan-Dependent interactions in Cancer","resumen":"Aberrant glycosylation, a common feature associated with malignancy, has beenimplicated in important events during cancer progression. Our understanding of the role of glycans in cancer has grown exponentially in the last few years, concurrent with important advances in glycomics and glycoproteomic technologies, paving the way for the validation of a number of glycan structures as potential glycobiomarkers. However, the molecular bases underlying cancer-associated glycan modifications are still far from understood. Glycans exhibit a natural heterogeneity, crucial for their diverse functional roles as specific carriers of biologically relevant information. This information is decoded by families of proteins named lectins, including sialic acid-binding immunoglobulin (Ig)-like lectins (siglecs), C-type lectin receptors (CLRs), and galectins. Siglecs are primarily expressed on the surface of immune cells and differentially control innate and adaptive immune responses. Among CLRs, selectins are a family of cell adhesion molecules that mediate interactions between cancer cells and platelets, leukocytes, and endothelial cells, thus facilitating tumor cell invasion and metastasis. Galectins, a family of soluble proteins that bind &#946;-galactoside-containing glycans, have been implicated in diverse events associated with cancer biology such as apoptosis, homotypic cell aggregation, angiogenesis, cell migration, and tumor-immune escape. Consequently, individual members of these lectin families have become promising targets for the design of novel anticancer therapies. During the past decade, a number of inhibitors of lectin?glycan interactions have been developed including small-molecule inhibitors, multivalent saccharide ligands, and more recently peptides and peptidomimetics have offered alternatives for tackling tumor progression. In this article, we review the current status of the discovery and development of chemical lectin inhibitors and discuss novel strategies to limit cancer progression by targeting lectin?glycan interactions.","palabras_clave":"glycans|cancer|siglecs|C-type lectins|selectins|galectins"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5862,"idioma_id":4,"titulo":"The pineal complex: a morphological and immunohistochemical comparison between a tropical (Paracheirodon axelrodi) and a subtropical (Aphyocharax anisitsi) characid species","resumen":"Cardinal neon Paracheirodon axelrodi andbloodfin tetra Aphyocharax anisitsi are two species ofcharacids with high trade value as ornamental fish inSouth America. Although both species inhabit middlewater layers, cardinal neon exhibits a tropical distributionand bloodfin tetra a subtropical one. In this work, we carriedout an anatomical, histological and immunohistochemicalstudy of the pineal complex of P. axelrodi and A.anisitsi. In both species, the pineal complex consisted ofthree components, the pineal and parapineal organs andthe dorsal sac (DS). The pineal organ was composed of ashort, thin pineal stalk (PS), vertically disposed withrespect to the upper surface of the telencephalon, and apineal vesicle (PV), located at the distal end of the PS andattached to the skull by connective tissue. The pineal window(PW), a site in the skull where the luminal informationaccesses the pineal organ, appeared just above thelatter structures. In the epidermis of P. axelrodi?s PW, clubcells were identified, but were not observed in the epidermisof A. anisitsi?s one. With respect to the DS, itappeared to be folded on itself, and was bigger and morefolded in A. anisitsi than in P. axelrodi. Immunohistochemicalassays revealed the presence of cone opsin-likeand rod opsin-like photoreceptor cells in the PS and PV.These results provide a first insight into the morphologicalassembly of the pineal complex of both species, and contributeto a better understanding of the integration andtransduction of light stimuli in characids","palabras_clave":"PINEAL|CHARACIDAE|RODOPSIN|BRAIN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5863,"idioma_id":4,"titulo":"Tardigrade diversity, an evaluation of natural and disturbed environments of the province of Salta (Argentina)","resumen":"Argentine tardigrades are relatively unknown and, in many cases, unstudied; in the province of Salta in northwesternArgentina the lone record of tardigrade dates to the 1980s. Here, we evaluate and compare tardigradediversity in natural habitats (N), urban environments (U) and rural communities (R) of Yungas. This work seeksto verify the existence of a reduction in tardigrade diversity outside their native habitats and if there is a biotichomogenization in the urban communities. Tardigrade community assemblages were compared between habitatsusing non-metric multidimensional scaling and a multi-response permutation procedure. Beta diversity wasanalysed in its component parts, species turnover and nesting, using a modified Raup?Crick test. Gammadiversity was divided into alpha and several beta levels. In total, 2080 eutardigrade and heterotardigradespecimens were registered and showed differences in tardigrade assemblages between habitats; the most diversehabitat was R. The beta diversity partition showed a nesting pattern in the U communities, highlighting faunalhomogenization, while species turnover was key in the R and N communities. There was species loss from N to Ucommunities, most markedly between R and U; various forces (both stochastic and deterministic) influence thestructure of these communities.","palabras_clave":"BETA DIVERSITY|BIOTIC HOMOGENIZATION|COMMUNITY CHANGES|NESTED PATTERN|RURAL HABITATS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5864,"idioma_id":4,"titulo":"Identification of novel transcriptional regulators of PKA subunits in Saccharomyces cerevisiae by quantitative promoter-reporter screening","resumen":"The cAMP-dependent protein kinase (PKA) signaling is a broad pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. However, how PKA achieves the cAMP-signal transduction specificity is still in study. The regulation of expression of subunits of PKA should contribute to the signal specificity. Saccharomyces cerevisiae PKA holoenzyme contains two catalytic subunits encoded by TPK1, TPK2 and TPK3 genes, and two regulatory subunits encoded by BCY1 gene. We studied the activity of these gene promoters using a fluorescent reporter synthetic genetic array screen, with the goal of systematically identifying novel regulators of expression of PKA subunits. Gene ontology analysis of the identified modulators showed enrichment not only in the category of transcriptional regulators, but also in less expected categories such as lipid and phosphate metabolism. Inositol, choline and phosphate were identified as novel upstream signals that regulate transcription of PKA subunit genes. The results support the role of transcription regulation of PKA subunits in cAMP specificity signaling. Interestingly, known targets of PKA phosphorylation are associated with the identified pathways opening the possibility of a reciprocal regulation. PKA would be coordinating different metabolic pathways and these processes would in turn regulate expression of the kinase subunits.","palabras_clave":"PKA|transcription regulation|TPKs|Bcy1|Saccharomyces cerevisiae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5865,"idioma_id":5,"titulo":"AHORROS EN LOS CONSUMOS PARA ENFRIAMIENTO DE ESPACIOS INTERIORES MEDIANTE LA EVALUACIÓN DE SISTEMAS DE PROTECCIÓN SOLAR EN EDIFICIOS RESIDENCIALES EN ALTURA. EL CASO DE LA ?CIUDAD-OASIS? DE MENDOZA, ARGENTINA","resumen":"El presente trabajo estudia el comportamiento térmico y energético de edificios residenciales en altura, en el contexto particular de una ?ciudad-oasis?. Se plantea como objetivo la definición de sistemas de control solar adecuados en la envolvente para cada tipología material de edificio -másica y liviana- y para cada estrato en altura de las viviendas: debajo y sobre de la copa de los árboles. El monitoreo de viviendas en uso permite validar modelos geométricos (software Energy Plus) y simular propuestas de aplicación de elementos de protección solar. Se analizan los ahorros en los consumos para enfriamiento en departamentos en niveles bajos, medios y altos. Los resultados muestran que en el edificio másico, en las viviendas ubicadas debajo de la copa de los árboles, es posible anular las cargas energéticas para enfriamiento mediante el uso de elementos exteriores de protección solar. Mientras que en las viviendas sobre la copa de los árboles, los ahorros energéticos se encuentran entre el 32% y 42%. En el edificio liviano, la aplicación de sistemas de control en departamentos bajo la copa de los árboles alcanza ahorros del orden del 53% y en las viviendas sobre la copa, las reducciones energéticas se encuentran entre el 46% y 54%. Las dimensiones adecuadas de tales elementos pueden generar propuestas posibles de ser transferidas a las normativas locales de edificación.","palabras_clave":"ARQUITECTURA AMBIENTAL|AHORRO|PROTECCIÓN SOLAR|EDIFICIO EN ALTURA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5866,"idioma_id":4,"titulo":"Response to Letter to the Editor 'On the targets of fluoxetine'","resumen":"Letter to the EditorResponse to Letter to the Editor ?On the targets of fluoxetine?","palabras_clave":"ANTIDEPRESSANT|FLUOXETINE|SEROTONIN|IMMUNOMODULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5867,"idioma_id":4,"titulo":"Molecular mechanisms of protein-cholesterol interactions in plasma membranes: Functional distinction between topological (tilted) and consensus (CARC\/CRAC) domains","resumen":"The molecular mechanisms that control the multiple possible modes of protein association with membrane cholesterol are remarkably convergent. These mechanisms, which include hydrogen bonding, CH-&#960; stacking and dispersion forces, are used by a wide variety of extracellular proteins (e.g. microbial or amyloid) and membrane receptors. Virus fusion peptides penetrate the membrane of host cells with a tilted orientation that is compatible with a transient interaction with cholesterol; this tilted orientation is also characteristic of the process of insertion of amyloid proteins that subsequently form oligomeric pores in the plasma membrane of brain cells. Membrane receptors that are associated with cholesterol generally display linear consensus binding motifs (CARC and CRAC) characterized by a triad of basic (Lys\/Arg), aromatic (Tyr\/phe) and aliphatic (Leu\/Val) amino acid residues. In some cases, the presence of both CARC and CRAC within the same membrane-spanning domain allows the simultaneous binding of two cholesterol molecules, one in each membrane leaflet. In this review the molecular basis and the functional significance of the different modes of protein-cholesterol interactions in plasma membranes are discussed.","palabras_clave":"membrane|cholesterol|lipid-protein interaction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5868,"idioma_id":4,"titulo":"Persistent organic pollutants (POPs) in fish with different feeding habits inhabiting a shallow lake ecosystem","resumen":"The occurrence of persistent organic pollutants (POPs) in the environment can affect organisms inhabitingaquatic systems, in particular shallow lakes that are vulnerable to environmental stressors. This studyaimed to assess POPs accumulation and changes at histological and physiological levels in tissues of threefish species with different trophic habits. Gills, brain, muscle, liver and gonads of Odontesthes bonariensis,Oligosarcus jenynsii and Cyphocharax voga were collected from the shallow lake La Peregrina, located inan agricultural area from Argentina. In addition, contaminant levels in surface water (SW), suspended particulatematter(SPM) and bottomsediments (BS) were assessed. Histological lesions were evaluated in fishtissues and levels of vitellogenin (VTG) were assessed in plasma of male fish in order to correlate these alterationswith the presence of POPs in the environment. Organochlorine pesticides (OCPs), polychlorinatedbiphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) were determined by GC-ECD. Biotic and abioticsamples showed the same POPs distribution pattern: OCPs N PCBs N PBDEs. Although tissue distributionof OCPs was species-specific, muscle showed the lowest levels in all species. The most abundant contaminantswere endosulfans, suggesting their widespread use in the area. O. bonariensis showed the highest","palabras_clave":"FISH|POP|SHALLOW LAKE|ECOSYSTEM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5869,"idioma_id":4,"titulo":"Reduced food interaction and enhanced gastrointestinal tolerability of a new system based on risedronate complexed with eudragit E100. Mechanistic approaches from in vitro and in vivo studies","resumen":"Novel complexes consisting of Eudragit E100-risedronate are presented. The oral bioavailability of risedronatein rats was determined through its percentage excreted in urine after administration of complexedor free risedronate in fed and fasted conditions. The evaluation of the risedronate gastroduodenalirritation potential was carried out by macroscopic and histological analyses in an experimentalrat model. The degree of counterionic condensation between Eudragit E100 and risedronate was assessedby dialysis with, mechanistic information about the interaction with calcium and the release of risedronatefrom the complexes being obtained using physiological solution and simulated gastric fluid withoutpepsin. Non-significant differences were observed in the urinary excretion of risedronate when thecomplex or free risedronate was administered to fasted rats. However, the urinary excretion of risedronatein the complex group was 4-times higher than in the free risedronate group when animals wereconcomitantly administered with food. This behavior was related to the high degree of counterionic condensationin the complex (86.5%), which led to a reduction in the calcium induced rate and magnitude ofrisedronate precipitation and resulted in a decrease in the gastroduodenal damage from the complex, asevidenced by a lower frequency of gastric mucosae hemorrhage. A sustained release of risedronate fromthe complex was observed toward water, simulated gastric fluid or physiological solution, through anionic-exchange mechanism. In conclusion, complexation with Eudragit E100 could be a useful strategyto overcome the unfavorable properties of risedronate.","palabras_clave":"Risedronate|Eudragit E|Complex|Bioavailability enhancement|Gastric damage|Food interaction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5870,"idioma_id":4,"titulo":"Psychosocial risk of fossilization by occupationally-used non-native English in information and communication technologists of Argentina","resumen":"Aims. Companies use non-native language as a service tool, and they may incur in occupational psychosocial risks. Interlanguage can be chronic under poor communicative situations, leading to fossilization. It could be an adverse effect because of its impact in productivity and occupational health. Thus, our aim was to establish factors of this psychosocial risk.Methods. 348 information and communication technologists (ICT) were analyzed. They were native Spanish speakers with normal hearing, and used English as a work tool. Age, gender, L2 stages and errors were recorded in relation to fossilization risk. Statistical methods were applied for categorical data (p<0.05).Results. After gender and age adjustments, a significant inverse association was found between L2 stages and fossilization risk (p<0.0001), with higher risk being in the acquisition stage. Also, L2 errors showed a significant direct relation with fossilization risk (p=0.0005).Conclusions. Summing up, ICT in acquisition L2 had upper psychosocial risk to fossilization with mechanistic execution of it, under poorer communicative formats. This results have high sanitary impact given they involved a massively demanded professionals.","palabras_clave":"BILINGUISMO |COGNICION|FOSILIZACION|SALUD LABORAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5871,"idioma_id":4,"titulo":"Artificial perches promote vegetation restoration","resumen":"Native ecosystems are continuously beingtransformed mostly into agricultural lands. Simultaneously, a large proportion of fields are abandonedafter some years of use. Without any intervention,altered landscapes usually show a slow reversion tonative ecosystems, or to novel ecosystems. One of themain barriers to vegetation regeneration is poorpropagule supply. Many restoration programs havealready implemented the use of artificial perches inorder to increase seed availability in open areas wherebird dispersal is limited by the lack of trees. Toevaluate the effectiveness of this practice, we performed a series of meta-analyses comparing the use ofartificial perches versus control sites without perches.We found that setting-up artificial perches increasesthe abundance and richness of seeds that arrive inaltered areas surrounding native ecosystems. Moreover, density of seedlings is also higher in open areaswith artificial perches than in control sites withoutperches. Taken together, our results support the use ofartificial perches to overcome the problem of poorseed availability in degraded fields, promoting and\/oraccelerating the restoration of vegetation in concordance with the surrounding landscape.","palabras_clave":"POVERTY|CHILDREN|COGNITIVE DEVELOPMENT|REVIEW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5872,"idioma_id":5,"titulo":"Estimulación de procesos cognitivos de control en niños de 4 años: comparaciones entre  formatos individual y grupal de intervención","resumen":"El presente trabajo analiza el impacto de dos modalidades de estimulación cognitiva (implementadas en el año 2005) en el desempeño en tareas con demanda de atención, memoria de trabajo, flexibilidad cognitiva y planificación. Los niños de 4 años de edad fueron estimulados durante cuatro meses con dos modalidades de estimulación cognitiva: individual (n=49), que fue diseñada en base a un programa de intervención previo; y grupal (n=143), que fue diseñada con el  objetivo de disminuir la tasa operadores\/niños, así como para reducir la brecha entre experiencias de entrenamiento de laboratorio y contextos educativos. Los resultados sugieren que: (a) ambas modalidades promovieron incrementos del desempeño cognitivo; y (b) existieron diferencias según el proceso cognitivo: en el caso de atención, los niños que participaron de la modalidad grupal tuvieron un menor incremento, mientras que en el caso de las tareas de memoria y planificación, el incremento de este mismo grupo fue mayor.","palabras_clave":"DESARROLLO COGNITIVO|INTERVENCIÓN|NIVEL SOCIOECONÓMICO|EDUCACIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5873,"idioma_id":4,"titulo":"Diet composition and foraging habitats of Adélie and gentoo penguins in three different stages of their annual cycle","resumen":"We evaluated the diet of Adélie (Pygoscelis adeliae) and gentoo (P. papua) penguins at Stranger Point colony (25 de Mayo\/King George Island) during different stages of their annual cycle using the stable isotope method and the conventional dietary analyses (i.e. stomach contents). Antarctic krill (Euphausia superba) dominated the diet of Adélie and gentoo penguins in all three studied stages (pre-breeding, breeding and post-breeding). Nevertheless, only in gentoo penguins a shift in the diet was evident from mainly krill during the breeding stage to mixed diet (i.e. krill and fish\/squids) during the pre- and post-breeding stages. Results from stable isotopes suggest that Adélie penguins might be foraging in southern localities compared to their congener during the inter-breeding period. Moreover, Adélie penguins showed a large individual variability in foraging habitat during the post-breeding stage. However, our model has predicted that both species exploited similar areas during the breeding season. The results obtained during the chick-rearing stage were compared with the stomach contents of both species obtained during the same season (2011\/2012). Our findings provide new knowledge on the feeding ecology and foraging habitats of pygoscelid penguins. They confirm the importance of Antarctic krill during the annual cycle and improve the understanding of life strategies and predator?prey interactions essentially out of the reproductive period, in which the information is very limited. These results can help to establish new management strategies, particularly considering the potential overlap between predators and fisheries.","palabras_clave":"PENGUINS|ANTARCTICA|ISOTOPES|TROPHIC ECOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5874,"idioma_id":4,"titulo":"Paleobiology of sabretooth cat Smilodon populator in the Pampean Region (Buenos Aires Province, Argentina) around the Last Glacial Maximum: Insights from carbon and nitrogen stable isotopes in bone collagen","resumen":"The sabretooth cat Smilodon populatorwas the largest felid in South America. It appears in the fossil record in theEarly Pleistocene, as an immigrant from North America, and becomes extinct around the Pleistocene?Holoceneboundary. The carbon and nitrogen stable isotopic values of collagen were measured for skeletal remains fromSmilodon specimens ranging in age from 25 to 10 kyr BP, for the first time in the Pampas region of Argentina.By comparison with similar values obtained on co-eval predators such as Protocyon (large canid) and Pantheraonca (jaguar) and a range of potential prey, such as giant ground sloths, glyptodontids, Macrauchenia, Toxodon,equids, cervids, and rodents, it could be established that Smilodon consumed essentially large prey from openlandscape, such as Macrauchenia and giant ground sloths during the last 15,000 years of the Late Pleistocene inthe Pampa region. It was possibly competing with the large canid Protocyon but the jaguar was apparently feedingon smaller size prey. A more humid climate at the beginning of the Holocene might have been unfavorable tothis large predator and could have contributed to its extinction. These results also provide an important insight tounderstand the ecological processes involved in the Great American Biotic Interchange.","palabras_clave":"Carbon-13|Nitrogen-15|Collagen|Smilodon"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5875,"idioma_id":4,"titulo":"PI3K pathway is involved in ERK signaling cascade activation by histamine H2R agonist in HEK293T cells","resumen":"p { margin-bottom: 0.25cm; line-height: 120%; }BACKGROUND:Histamine, throughhistamine H2 receptor (H2R), modulates different biologicalprocesses, involving the modulation of PI3K\/AKT\/mTOR andRAS\/RAF\/MEK\/ERK pathways. Many evidences have demonstrated theexistence and importance of the crossregulation between these twosignaling pathways. The aim of the present work was to determine themolecular mechanisms leading to PI3K and ERK pathways modulationinduced by the H2R agonist amthamine and to evaluate the possibleinterplay between them.METHODS:Phosphorylationlevels of ERK and Akt were examined by Western blot in HEK293T cellsexpressing the human H2R, in the presence of H2R agonist and dominantnegative mutants or pharmacological inhibitors of differentproteins\/pathways. Transcriptional activity assays were assessed todetermine SRE activity. Amthamine-mediated cellular proliferation wasinvestigated in MA-10A cells in the presence of PI3K inhibitor.RESULTS:H2R agonist inhibitsPI3K\/Akt\/mTOR and stimulates Ras\/MEK\/ERK pathways. Moreover,PI3K\/Akt\/mTOR signaling inhibition is necessary to achieve H2Rmediated ERK activation. In the presence of a constitutive activemutant of Akt, amthamine is not able to mediate ERK activation. Thiscrosstalk affects classical ERK downstream targets such as Elk1phosphorylation and the transcriptional activity of the SRE,classically associated to proliferation. We further demonstrate thatamthamine-induced proliferation in Leydig MA-10 tumor cells, isenhanced by LY294002, a PI3K inhibitor.CONCLUSIONS:These resultsdescribe a crosstalk between PI3K\/AKT\/mTOR and Ras\/MEK\/ERK pathwaysinduced by H2R stimulation with implications in cell proliferation.GENERALSIGNIFICANCE:This work indicatesthat the modulation of PI3K\/AKT\/mTOR pathway by H2R in turn regulatesRas\/MEK\/ERK activation conditioning the proliferative capacity of thecells.","palabras_clave":"Histamine|signalling|cell proliferation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5876,"idioma_id":4,"titulo":"Breaking the rule: multiple patterns of scaling of sexual size  dimorphism with body size in orthopteroid insects","resumen":"Sexual size dimorphism (SSD) although a widespread phenomenon among animals, is both enigmatic as to its proximate and ultimate causes and the scaling relationships between SSD and body size (Rensch?s rule). We analyzed SSD at the intra- and interspecific levels in a number of representative species and genera of the major orthopteroid orders: Orthoptera, Phasmatodea, Mantodea, Blattodea, Dermaptera, Isoptera, and Mantophasmatodea. The vast majority of the species showed female biased SSD but numerous exceptions occur in cockroaches and earwigs. Rensch?s rule and its converse are not common patterns at both, intraand cross-species level, most species and genera showing an isometric relationship between male and female body sizes. In some but not all cases, the demonstrated allometric patterns could be related to geographic body size variation. We also showed that not all body size estimators produce the same degree of SSD and that dimorphism can be strongly influenced by a number of living conditions and the patterns of nymphal development. Finally, we discuss our results in relation to current models of the evolution of sexual size dimorphism in animals.","palabras_clave":"Body size|Orthopteroid|Rensch's Rule|Scaling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5877,"idioma_id":4,"titulo":"Arsenic stress induces changes in lipid signalling and evokes the stomata closure in soybean","resumen":"Soybean (Glycine max) is often exposed to high arsenic (As) level in soils or through irrigation with groundwater. In previous studies on As-treated soybean seedlings we showed deleterious effect on growth, structural alterations mainly in root vascular system and induction of antioxidant enzymes. However, there are not reports concerning signal transduction pathways triggered by the metalloid in order to develop adaptive mechanisms. Phosphatidic acid (PA), a key messenger in plants, can be generated via phospholipase D (PLD) or via phospholipase C (PLC) coupled to diacylglycerol kinase (DGK). Thus, changes in PA and in an enzyme involved in its metabolism (PLD) were analysed in soybean seedlings treated with 25 mM AsV or AsIII. The present study demonstrated that As triggers the PA signal by PLD and also via PLC\/DGK mainly after 48 h of As treatment. DGPP, other lipid messenger produced by phosphorylation of PA by PAK increased in As treated roots. Arsenic also induced rapid and significant stomatal closure after 1.5 h of treatment, mainly with AsIII, probably as an adaptive response to the metalloid to reduce water loss by transpiration. This report constitute the first evidence that shows the effects of As on lipid signalling events in soybean seedlings which would be crucial in adaptation and survival of soybean seedlings under As stress. ","palabras_clave":"GLYCINE MAX|PHOSPHATIDIC ACID|PHOSPHOLIPASE D|ARSENIC ACIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5878,"idioma_id":4,"titulo":"Hybrid sol-gel coatings containing clay nanoparticles for corrosion protection of mild steel","resumen":"The development of a new environment-friendly anticorrosive coating for mild steel substrate is afforded in this work. The combined use of cerium, as a self-healing agent, and clay nanoparticles, as improvers of the barrier properties, was considered to the development of new anticorrosive sol-gel coatings. Nanostructured hybrid films were synthesized by the sol?gel route from tetraethoxysilane (TEOS) and 3-glicidoxipropyl-trimetoxisilano (GPTMS) using laminar nanoclays (Laponite Na+0.7[Si8Mg5.5Li0.3H4O24]&#8722;0.7) to improve mechanical and barrier properties, and Ce(NO3)3?6H2O as a supplier of Ce(III) to provide an inhibiting effect in the event of coating failure. Carbon steel plates, AISI 1010, were used as substrates. Prior to the application of the coating, samples were treated with a phosphoric acid 2% v\/v in order to improve coating adherence.In order to evaluate cerium effect, electrochemical behaviour of films containing Laponite and cerium salts (TGL-Ce) were compared with films containing only Laponite (TGL) by means of potentiodynamic polarization tests and electrochemical impedance spectroscopy (EIS) measurements using a 0.35 wt% NaCl solution. Microstructural characterization and surface analysis of substrates and sol-gel coatings were performed by optical microscopy and by XPS techniques. The use of nanoclays allowed to achieve a significant improvement of the anticorrosive behaviour of the cerium doped coating at the same time that enhances the physical integrity of the coatings under immersion tests.","palabras_clave":"MILD STEEL|CERIUM|CORROSION|SILANES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5879,"idioma_id":4,"titulo":"Ancient DNA from the extinct South American giant glyptodont Doedicurus sp. (Xenarthra: Glyptodontidae) reveals that glyptodonts evolved from Eocene armadillos","resumen":"Glyptodonts were giant (up to ~2,400 kg), heavily armoured relatives of living armadillos, which became extinct during the Late Pleistocene\/early Holocene alongside much of the South American megafauna. Although glyptodonts were an important component of Cenozoic South American faunas, their early evolution and phylogenetic affinities within the order Cingulata (armoured New World placental mammals) remain controversial. In the present study we used hybridisation enrichment and high-throughput sequencing to obtain a partial mitochondrial genome from Doedicurus sp., the largest (1.5m tall, and 4m long) and one of the last surviving glyptodonts. Our molecular phylogenetic analyses revealed that glyptodonts fall within the diversity of living armadillos. Reanalysis of morphological data using a molecular 'backbone constraint' revealed several morphological characters that supported a close relationship between glyptodonts and the tiny extant fairy armadillos (Chlamyphorinae). This is surprising as these taxa are among the most derived cingulates: glyptodonts were generally large-bodied and heavily armoured, while the fairy armadillos are tiny (~9-17 cm) and adapted for burrowing. Calibration of our phylogeny with the first appearance of glyptodonts in the Eocene resulted in a more precise timeline for xenarthran evolution. The osteological novelties of glyptodonts and their specialisation for grazing appear to have evolved rapidly during the Late Eocene to Early Miocene, coincident with global temperature decreases and a shift from wet closed forest towards drier open woodland and grassland across much of South America. This environmental change may have driven the evolution of glyptodonts, culminating in the bizarre giant forms of the Pleistocene. This article is protected by copyright. All rights reserved.","palabras_clave":"ancient DNA|Glyptodonts|Doedicurus |South American"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5880,"idioma_id":4,"titulo":"Alumina-supported nickel onto cordierite monoliths for ethane oxydehydrogenation: Coating strategies and their effect on the catalytic behavior","resumen":"Different strategies for the generation of Ni (15 wt.%)\/Al2O3 catalytic coatings onto cordierite monoliths applied to the oxidative dehydrogenation of ethane (ODHE) were investigated. Strategy 1 involved the deposition of an alumina layer (nanoparticles or micro + nanoparticles) and the later incorporation of the active phase. Strategy 2 employed a suspension containing a powder catalyst that was used to deposit the catalytic film. Morphological (SEM) and physicochemical (LRS and XPS) characterizations were used to explain the catalytic performance of the structured systems. The NiO particles distribution on the catalytic coatings obtained was analyzed via SEM-EDX performed in different monolith regions. The ultrasonic test was used to evaluate the anchoring of these catalytic films to the substrates. It was concluded that the selection of both the strategy and the particle size of the support is extremely important in order to obtain a homogeneously dispersed, accessible and highly adhered to the substrate catalyst.","palabras_clave":"CORDIERITE MONOLITHS|COATING STRATEGIES|ALUMINA-SUPPORTED NICKEL|ODH ETHANE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5881,"idioma_id":4,"titulo":"Development of sepiolite\/SiC porous catalytic filters for diesel soot abatement","resumen":"Honeycomb monoliths made up of 70 wt.% sepiolite and 30 wt.% SiC were prepared and macropores were generated in the honeycomb structure by introducing a temporary additive with the aim of obtaining an adequate porosity to use the structures prepared as diesel soot filters (DPF). CLARIMEX activated carbon was the most suitable among the additives studied. In order to generate wall-flow type filters, it was also necessary to investigate suitable materials to alternatively plug the channels of the honeycomb monolith, finding that refractory mortar finely milled and mixed with water formed a paste easy to apply, which adhered firmly to the walls of the monolith being thermally and mechanically resistant. The incorporation of Co and Ce as active phases was performed by successive impregnation followed by calcination at 600°C. This system presented satisfactory results with respect to the maximum temperature of soot combustion rate, which is in the range of the diesel exhaust gases temperature (300-450°C). The wall-flow catalytic monoliths developed have similar characteristics to commercial diesel particulate filters in spite of having 70 wt.% of natural clay in its composition. The pore size remains to be further modulated in order to achieve a better permeation flow, similar to that of commercial filters, which could be tried out using larger particle size activated carbons.","palabras_clave":"DIESEL PARTICULATE FILTER|WALL-FLOW MONOLITH|SEPIOLITE AND SiC|PORE-GENERATING AGENTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5882,"idioma_id":4,"titulo":"Flexible NiZr-based structured catalysts for ethylene production through ODH of ethane: Catalytic performance enhancement","resumen":"Flexible structured catalysts based on Ni and Zr deposited on a ceramic paper made of SiO2-Al2O3andZrO2fibers showed to be active and selective for the Oxidative Dehydrogenation of Ethane. Zirconiumpromoter strongly interacted with nickel oxide and produced structural changes that affected the NiOredox behavior, crystal growth and lattice order which enhanced selectivity towards ethylene. The varia-tion of the concentration of the precursor solution used and the addition of zirconia short fibers, affectedthe catalytic performance. The lower the concentration of the solution used to incorporate the catalyst,the better the catalytic performance. The addition of zirconia fibers also showed beneficial effects on ethylene production.","palabras_clave":"OXIDATIVE DEHYDROGENATION OF ETHANE|Ni CATALYSTS|Zr PROMOTER|CATALYTIC CERAMIC PAPER|ETHYLENE PRODUCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5883,"idioma_id":4,"titulo":"Structures of Trichloromethyl Thiocyanate, CCl3SCN, in Gaseous and Crystalline State","resumen":"Trichloromethyl thiocyanate, CCl3SCN, was structurally studied in both the gas and crystal phases by means of gas electron diffraction (GED) and single-crystal X-ray diffraction (XRD), respectively. Both experimental studies and quantum chemical calculations indicate a staggered orientation of the CCl3 group relative to the SCN group. This conclusion is supported by the similarity of the C-SCN bond length to that of the anti-structure of CH2ClSCN (Berrueta Mart&iacute;nez et al. Phys. Chem. Chem. Phys. 2015, 17, 15805-15812). Bond lengths and angles are similar for gas and crystal CCl3SCN structures; however, the crystal structure presents different intermolecular interactions. These include halogen and chalcogen type interactions, the geometry of which was studied. Characteristic C-Y&#8943;N angles (Y=Cl or S) close to 180&deg; provide evidence for typical &sigma;-hole interactions along the halogen\/chalcogen-carbon bond in N&#8943;Cl and N&#8943;S, intermolecular units. Crystallize the difference: The crystal and gas-phase structures of CCl3SCN are studied by X-ray and gas electron diffraction techniques. In both phases, the CCl3 group presents a staggered orientation relative to the SCN group. Different intermolecular interactions are detected in the solid phase, including halogen- and chalcogen-type interactions.","palabras_clave":"X-ray Diffraction|Gas electron diffraction|Crystal structure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5884,"idioma_id":4,"titulo":"Comment on ??Structural and vibrational studies on 1-(5-Methyl-[1,3,4] thiadiazol-2-yl)-pyrolidin-2-ol?? [Spectrochimica Acta Part A, 152 (2016) 252?261]","resumen":"The title paper [1] reports a study on the spectroscopic and  Physicochemical properties of 1-(5-methyl- [1,3,4]thiadiazol-2-yl)-pyrrolidin-2-ol (MTPN) based on experimental and theoretical data. The latter ones are based on the computed molecular structure for a rather unusual conformer. Here, after a careful analysis of the conformational space of MTPN, the most stable conformation was determined for the molecule isolated in a vacuum, which results to be 21.9 kJ\/mol more stable than the conformer reported previously. Our study also includes the closely related species 1-(5-trifluoromethyl- [1,3,4]thiadiazol-2-yl)-pyrrolidin-2-ol (FMTPN). An intramolecular OH&#8943;N hydrogen bond determines the conformational behavior of the [1,3,4]thiadiazol-2-yl)-pyrrolidin-2-ol group as demonstrated by Natural Bond Orbital population analysis.","palabras_clave":"CONFORMATION|HYDROGEN BOND"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5886,"idioma_id":5,"titulo":"Modelos experimentales en nefrología. Modelo de riñón aislado y perfundido","resumen":"El modelo de riñón aislado y prefundido brinda la posibilidad de analizar la funciónrenal bajo condiciones definidas y sin la intervención de factores sistémicos. Estemodelo consiste en aislar al riñón de la vasculatura sistémica, y perfundirlo a travésde la arteria renal con un medio de perfusión con características similares al plasmao a la sangre, conservando las características anatómicas, bioquímicas y funcionalesdel riñón intacto. Esto posibilita circunscribir factores extrarrenales que puedencomplicar la interpretación de la información obtenida con experimentos realizadosin vivo. Además, la utilización del órgano aislado permite el estudio de la funciónrenal bajo condiciones en las que el operador puede modificar las variables a analizarde manera controlada. En el presente artículo se revisa la técnica de preparación,los criterios de viabilidad, las principales características hemodinámicas y tubulares,y las aplicaciones del modelo.","palabras_clave":"modelos experimentales|riñón aislado"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5887,"idioma_id":4,"titulo":"Morpho-physiological response to drought of progenies of Pinus taeda L. contrasting in mean growth rate","resumen":"Potential trade-offs between growth and water stress resistance in Pinus taedaL. families, and the ecophysiological processes involved were evaluated. Families of high(HG) and low (LG) growth rate were subject to moderate and severe water stress. Familiesonly differed in growth under the high water availability condition (control treatment),declining exponentially to similar values in all families under drought. HG families presented higher absolute growth but lower relative growth than LG families. Hydraulicconductivity of branches decreased at high air saturation deficit (D) even with high soilwater content due to high vulnerability to cavitation (mean P50 = 1.7 MPa) and poorstomatal control of water potential in all families (which was even lower in LG). LGfamilies presented higher carbon fixation capacity (per unit leaf area) but this could not bereflected in absolute growth due to their higher biomass allocation to roots compared to HGfamilies. HG2 family allocated more biomass to leaves than the other families, increasingits whole capacity of C fixation. HG1 family had a differential allocation to the stem,probably increasing whole hydraulic conductance and capacitance, and therefore having anefficient way of providing water to the leaves. Our results does not support the hypothesisof a tradeoff between growth and drought resistance in Pinus taeda, but that there aredifferent combinations of traits, as those of HG families, leading to high absolute growthwhen soil water availability is high, and low growth?but similar to that of LG families?under water deficit conditions.","palabras_clave":"GENETIC VARIABILITY|LOBLOLLY PINE|RESOURCE USE EFFICIENCY|GROWTH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5888,"idioma_id":4,"titulo":"Relative DNA content in diploid, polyploid, and multiploid species of Paspalum (Poaceae) with relation to reproductive mode and taxonomy","resumen":"Abstract It is generally accepted that polyploids havedownsized basic genomes rather than additive values withrespect to their related diploids. Changes in genome sizehave been reported in correlation with several biologicalcharacteristics. About 75 % of around 350 species recognizedfor Paspalum (Poaceae) are polyploid and most polyploidsare apomictic. Multiploid species are common withmost of them bearing sexual diploid and apomictic tetraploidor other ploidy levels. DNA content in the embryo andthe endosperm was measured by flow cytometry in a seedby-seed analysis of 47 species including 77 different entities.The relative DNA content of the embryo informed thegenome size of the accession while the embryo:endospermratio of DNA content revealed its reproductive mode. Thegenome sizes (2C-value) varied from 0.5 to 6.5 pg and for29 species were measured for the first time. Flow cytometryprovided new information on the reproductive modefor 12 species and one botanical variety and supplied newdata for 10 species concerning cytotypes reported for thefirst time. There was no significant difference between themean basic genome sizes (1Cx-values) of 32 sexual and45 apomictic entities. Seventeen entities were diploid and60 were polyploids with different degrees. There were noclear patterns of changes in 1Cx-values due to polyploidyor reproductive systems, and the existing variations are inconcordance with subgeneric taxonomical grouping.","palabras_clave":"DNA CONTENT|GENOME SIZE|REPRODUCTION|APOMIXIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5889,"idioma_id":4,"titulo":"Immunohistochemical and ultrastructural evidence of functional organization along the Corydoras paleatus intestine","resumen":"The Neotropical catfish, Corydoras paleatus (Callichthyidae) is a facultative air-breathing teleost that makes use of the caudal portion of the intestine as an accessory air-breathing organ. This portion is highly modified, being well vascularized with capillaries between epithelial cells, which makes it well suited for gas exchange. Instead, the cranial portion is a digestion and absorption site, as it has a typical intestinal epithelium with columnar cells arranged in a single row, villi and less vascularized tunica mucosa. Therefore, the intestine was studied by light and electron microscopy to assess differences between the cranial, middle and caudal portions. In order to characterize the potential for cell proliferation of this organ, we used anti-proliferating cell nuclear antigen antibody and anti-Na+K+-ATPase monoclonal antibody to detect the presence of Na+\/K+ pump. In C. paleatus it was observed that cell dynamics showed a decreasing gradient of proliferation in cranio-caudal direction. Also, the intestine of this catfish is an important organ in ionoregulation: the basolateral Na+\/K+ pump may have an active role, transporting Na+ out of the cell while helping to maintain the repose potential and to regulate cellular volume.","palabras_clave":"NEOTROPICAL CATFISH|NA+ K+ ATPase|PCNA|AIR-BREATHING ORGAN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5890,"idioma_id":4,"titulo":"Why the pore geometry model could affect the uniqueness of the PSD in AC characterization","resumen":"In this paper we discuss why the pore geometry can affect the unicity of the pore size distribution (PSD) of a given activated carbon (AC) sample, when different probe gases are used in adsorption measures. In order to characterize the solid sample we used grand canonical Monte Carlo simulation and the independent pore model with slit or triangular pore geometry, focusing our analysis on the possibility of representing the adsorptive processes of a triangular pore of defined size by means of a combination of slit pores of different sizes. This representation is tested on experimental adsorption data of N2 (77 K) on AC samples and acceptable results were obtained. Finally, we have performed a theoretical test, which consisted of analyzing a virtual porous solid with this approach and different probe gases (N2 at 77 K and CO2 at 273 K), showing that the differences between the pore representations can cause differences between the solid representations for the adsorptive properties, for these different gases. The analysis presented here can be extended to other pore geometries and other adsorbates, and provide arguments to further explain results presented in our previous paper, which refers to cases when different adsorbates yield different PSDs for a given sample and the same pore geometry model.","palabras_clave":"AC characterization|psd|Monte Carlo SImularion|Pore geometry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5892,"idioma_id":5,"titulo":"ESTUDIOS ETNOBOTÁNICOS EN LA CONURBACIÓN BUENOS AIRES-LA PLATA, ARGENTINA: PRODUCTOS DE PLANTAS MEDICINALES INTRODUCIDOS POR INMIGRANTES PARAGUAYOS","resumen":"Este trabajo incluye resultados parciales de una línea de investigación en Etnobotánica urbana,que estudia los conocimientos locales sobre las plantas, sus partes y productos derivados en laconurbación Buenos Aires-La Plata. Se incluyen productos de plantas medicinales de recienteingreso en el área de estudio a través de inmigrantes oriundos de Paraguay. Los productoscorresponden a 10 especies: Achyrocline alata (?jateí-ka?á?), Baccharis gaudichaudiana(?jaguareté-ka?á?), Cuphea lysimachioides (?ysypó-peré?), Euphorbia serpens (?tupasý-kambý?),Genipa americana (?ñandypá?), Helietta apiculata (?yvyra ovy?), Lycopodiella cernnua (?itá-potý?), Rhynchosia balansae (?urusú-he?&#283;?), Stevia rebaudiana (?ka?á-he?&#283;?) y Victoria cruziana(?jacaré-yrupé?). Para cada caso se indican sus productos, muestras de referencia, usosasignados, actividad biológica y efectos estudiados. La presencia de los productos de estasespecies en el área metropolitana indica un aumento de la diversidad biocultural local, tanto deplantas y productos como de sus conocimientos asociados.","palabras_clave":"Contexto pluricultural urbano|etnobotánica urbana| migración paraguaya|productos vegetales terapéuticos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5893,"idioma_id":4,"titulo":"Exploring the positive allosteric modulation of human &#945;7 nicotinic receptors from a single-channel perspective","resumen":"Enhancement of &#945;7 nicotinic receptor (nAChR) function by positive allosteric modulators (PAMs) is a promising therapeutic strategy to improve cognitive deficits. PAMs have been classified only on the basis of their macroscopic effects as type I, which only enhance agonist-induced currents, and type II, which also decrease desensitization and reactivate desensitized nAChRs. To decipher the molecular basis underlying these distinct activities, we explored the effects on single-&#945;7 channel currents of representative members of each type and of less characterized compounds. Our results reveal that all PAMs enhance open-channel lifetime and produce episodes of successive openings, thus indicating that both types affect &#945;7 kinetics. Different PAM types show different sensitivity to temperature, suggesting different mechanisms of potentiation. By using a mutant &#945;7 receptor that is insensitive to the prototype type II PAM (PNU-120596), we show that some though not all type I PAMs share the structural determinants of potentiation. Overall, our study provides novel information on &#945;7 potentiation, which is key to the ongoing development of therapeutic compounds.","palabras_clave":"CYS-LOOP RECEPTORS|NICOTINIC RECEPTORS|PATCH-CLAMP|SINGLE-CHANNEL RECORDINGS|POSITIVE ALLOSTERIC MODULATORS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5894,"idioma_id":4,"titulo":"Critical study of the distribution of rotational velocities of Be stars - I. Deconvolution methods, effects due to gravity darkening, macroturbulence, and binarity","resumen":"Context. Among massive and of intermediate-mass stars, Be stars are the fastest rotators in the main sequence (MS), and as such, a cornerstoneto validate models of structure and evolution of rotating stars. However, several phenomena induce under- or over-estimations either of theirapparent Vsin i, or ?true? velocity V.Aims. In the present contribution we aim at obtaining distributions of true rotational velocities corrected for systematic eects induced by therapid rotation itself, macro-turbulent velocities, and binarity.Methods. We study a set of 233 Be stars by assuming they have inclination angles distributed at random. A critical discussion is carried on Cranmer?s and Lucy-Richardson?s methods that enable to transform a distribution of projected into another of true rotational velocities, where the gravitational darkening eect on the Vsin i parameter is considered in dierent ways. We conclude that Lucy-Richardson?s iterative algorithm responds at best to the purposes of the present work, but it requires a thorough determination of the stellar fundamental parameters.Results. We conclude that once the mode of ratios of true velocities of Be stars attains the value V=Vc ´ 0:77 in the MS evolutionary phase,it remains unchanged up to the end of the MS lifespan. When Be stars in all MS evolutionary stages are considered together, the statisticalcorrections carried on the distribution of ratios V=Vc for over-estimations of Vsin i due to macro-turbulent motions and binarity, produce a shiftof this distribution towards lower values of V=Vc. The mode of the final distribution obtained is at V=Vc ´ 0:65. It has a nearly symmetricdistribution and shows that the Be phenomenon is characterized by a wide range of true velocity ratios: 0:3 . V=Vc . 0:95. It Thus suggeststhat the probability for Be stars being critical rotators is extremely low.Conclusions. The corrections attempted in the present work represent a previous step to infer indications on the nature of the Be-star surfacerotation that will be studied in the second paper of this series.","palabras_clave":"Stars: emission-line, Be|Stars: rotation|Stars: macro-turbulence|Stars: binarity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5895,"idioma_id":4,"titulo":"Inhomogeneous molecular ring around the B[e] supergiant LHA 120-S 73.","resumen":"Context. B[e] supergiants are evolved massive stars, enshrouded in a dense wind and surrounded by a molecular and dusty disk. Themechanisms that drive phases of enhanced mass loss and mass ejections, responsible for the shaping of the circumstellar material ofthese objects, are still unclear.Aims. We aim to improve our knowledge on the structure and dynamics of the circumstellar disk of the Large Magellanic Cloud B[e]supergiant LHA 120-S 73.Methods. High-resolution optical and near-infrared spectroscopic data were obtained over a period of 16 and 7 years, respectively.The spectra cover the diagnostic emission lines from [Ca ii] and [O i], as well as the CO bands. These features trace the disk atdifferent distances from the star. We analyzed the kinematics of the individual emission regions by modeling their emission profiles.A low-resolution mid-infrared spectrum was obtained as well, which provides information on the composition of the dusty disk.Results. All diagnostic emission features display double-peaked line profiles, which we interpret as due to Keplerian rotation. We findthat the profile of each forbidden line contains contributions from two spatially clearly distinct rings. In total, we find that LHA 120-S 73 is surrounded by at least four individual rings of material with alternating densities (or by a disk with strongly non-monotonicradial density distribution). Moreover, we find that the molecular ring must have gaps or at least strong density inhomogeneities,or in other words, a clumpy structure. The optical spectra additionally display a broad emission feature at 6160?6180 Å, which weinterpret as molecular emission from TiO. The mid-infrared spectrum displays features of oxygen- and carbon-rich grain species,which indicates a long-lived, stable dusty disk.We cannot confirm the previously reported high value for the stellar rotation velocity. He i &#955; 5876 is the only clearly detectable pureatmospheric absorption line in our data. Its line profile is strongly variable in both width and shape and resembles of those seen innon-radially pulsating stars. A proper determination of the real underlying stellar rotation velocity is hence not possible.Conclusions. The existence of multiple stable and clumpy rings of alternating density recalls ring structures around planets. Althoughthere is currently insufficient observational evidence, it is tempting to propose a scenario with one (or more) minor bodies or planetsrevolving around LHA 120-S 73 and stabilizing the ring system, in analogy to the shepherd moons in planetary systems.","palabras_clave":"Circumstellar matter|Infrared: stars - stars: early-type|Stars: massive - supergiants|Stars: individual: LHA 120-S 73"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5896,"idioma_id":4,"titulo":"A native top predator relies on exotic prey inside a protected area: The puma and the introduced ungulates in Central Argentina","resumen":"Top predators play an important role to preserving healthy and functional ecosystems. Predatory interactions among generalist predators and native prey may be altered due to occurrence and availability of introduced prey species. These interactions seldom receive attention in biodiversity conservation, particularly when establishing protected area management guidelines. In this study we described puma (Puma concolor) diet in a protected area from central Argentina, where red deer (Cervus elaphus) and wild boar (Sus scrofa) were regionally introduced for game a hundred years ago. We aimed to evaluate if the puma effectively bases its diet on native prey species maintaining natural ecological interactions despite the occurrence and availability of these introduced prey species. We analyzed 83 puma scats in a landscape containing both native and introduced species susceptible to predation. Results indicate that puma diet was composed mostly by introduced species, which represented 80.8% of the total biomass consumed (Cervus elaphus 40.6%, Sus scrofa 39.4%, and Ovis aries 0.8%). Pumas mainly preyed on introduced ungulates in the protected area, where management guidelines do not account for puma-native prey interactions. We suggest implementation of management actions to reduce densities of these introduced ungulates to restore natural ecological interactions between the puma and native prey.","palabras_clave":"TOP PREDATOR|PUMA DIET|PUMA CONCOLOR|INTRODUCED SPECIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5897,"idioma_id":4,"titulo":"The importance of flood events on the establishment of seedlings and cuttings of saltcedar (Tamarix ramosissima)","resumen":"The expansion of invasive plants is considered one of the greatest threats to biodiversity. Theenvironments more threatened by the presence of exotic invasive species are the riparian areas due to theirsmall surface and high diversity. The advance of Tamarix in these environments may be linked to transientconditions of higher water availability, which would allow initial colonization. The objective of the presentwork was to determine which hydric conditions promote germination and establishment of Tamarix cuttingsby measuring in the laboratory the effects of different periods of flooding. It was observed that the germinationwas high under irrigation at field capacity and was decreased after 10 days of flood, while the establishmentthrough cuttings required at least 15 days duration of a flood event. These results will contribute to theidentification of critical environmental conditions that facilitate the establishment of the genus in new areasand guide actions for its early control.","palabras_clave":"biological invasion|stress|salinity|plants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5898,"idioma_id":4,"titulo":"Diving behavior of Southern Right Whales (Eubalaena australis) in a maritime traffic area in Patagonia, Argentina","resumen":"Southern right whales migrate annually from periantarctic foraging areas to mating and breeding areas in coastal waters. Península Valdés is internationally known as one of the most important breeding areas for this species. Even though Península Valdés does not constitute a feeding area, in recent years individuals have been observed swimming with their mouths open at the surface. Towards the end of the whale season, they move away from the coast to depths that range from 80 to 120 meters, where individuals perform long dives in an apparent feeding behavior. A total of five whales were tagged with a suction cup device containing a time-depth recorder (TDR) in Golfo Nuevo. Individuals tagged dived to depths close to 80 meters. Deep waters of Golfo Nuevo are used by ships and vessels as a route to enter to Puerto Madryn port, an industrial city, hence posing a possible threat to the whales, due to the risk of collision between whales and ships. Our aim was to report a first record of Southern right whale diving behaviors in order to know how this behavior could affect or threaten whales in ship traffic areas to and from the port close to Puerto Madryn city.","palabras_clave":"SOUTHERN RIGHT WHALE|BEHAVIOR|MARITIM TRAFFIC|BREEDING AREA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5899,"idioma_id":4,"titulo":"Longitudinal Taphonomic Studies of Mammal Carcasses from the Rio Salado Valley, Mendoza, Argentina","resumen":"Taphonomic studies aid in understanding the transition of organic matter from living contexts (the biosphere) togeological contexts (the lithosphere), and how agents and processes affect skeletal remains in the archaeological record.Archaeological research in the Atuel River valley, Mendoza, Argentina, has been carried out for several years. However,actualistic approaches to examine local and regional taphonomic processes have only been recently developed in this area.Longitudinal studies of disarticulation and dispersion of bones from carcasses are presented here in order to understandhow skeletal materials are naturally added to, subtracted from, and mixed within the archaeological record. In this paperwe explore variability in the influence of taphonomic processes in different microhabitats of the valley and how thesemechanisms impact the potential of mixing during bone deposition in the archaeological record. There are some qualitativedifferences between carcasses deposited in different landforms, showing that each place has its own taphonomic history,principally influenced by the geomorphic and ecologic processes that operate in different landforms.","palabras_clave":"Taphonomy|Actualistic studies|Mountain environments|Zooarchaeology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5900,"idioma_id":4,"titulo":"3. KOH-DOPED ABPBI MEMBRANE FOR ALKALINE WATER ELECTROLYSIS: CHARACTERIZATION AND PERFORMANCE","resumen":"The properties and performance of linear and cross-linked KOH doped ABPBI membranes as electrolyte\/separator for zero gap alkaline water electrolysis cells are evaluated and compared with a commercial Zirfon® diaphragm. Stability in alkaline environment, swelling, thermal properties, water sorption, KOH uptake and conductivity of linear (L-ABPBI) and cross-linked (C-ABPBI) membranes doped with different concentrations of KOH are analyzed. Linear membranes show stability up to 3.0 moldm-3 KOH doping,while cross-linked membranes are stable up to 4.2 moldm-3 KOH doping. Both kinds of membranes exhibit good thermal stability and reasonable specific ionic conductivity at 22 C in the range between 7 and 25 mScm-1, being slightly higher the conductivity of C-ABPBI membranes than that of L-ABPBI ones.In short-term electrolysis tests both L-ABPBI and C-ABPBI membranes show better performance than Zirfon diaphragm in the range from 50 to 70 C. A current density of 335 mAcm-2 at a cell voltage of 2.0 V is attained with C-ABPBI membranes doped in 3 mol$dm3 KOH at 70 C, a performance comparable with that of commercial units operating at temperatures ca. 80 C and 30 wt% KOH (6.7 moldm-3) as electrolyte.","palabras_clave":"ABPBI |MEMBRANES|ALKALINE |WATER ELECTROLYSIS|ELECTROLYZERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5901,"idioma_id":4,"titulo":"Control of Listeria monocytogenes biofilms on industrial surfaces by the bacteriocin-producing Lactobacillus sakei CRL1862","resumen":"The effect ofthe bacteriocin-producing Lactobacillussakei CRL1862 and its bacteriocin in the control of Listeria biofilm formation on industrial surfaces at 10 ºC, was investigated.A screening among different Listeria specieswas performed allowing selecting L.monocytogenes FBUNT for its use as biofilm producer on stainless steel (SS)and polytetrafluoroethylene (PTFE) surfaces. Three conditions were simulated toevaluate the ability of the bacteriocinogenic strain to displace, exclude andcompete pathogen biofilm formation. L. sakei CRL1862 effectively inhibitedbiofilm formation by L. monocytogenesFBUNT through the three assayed mechanisms, pathogen inhibition being more efficienton PTFE than on SS surface. Moreover, co-culture of L. monocytogenes FBUNT with the bacteriocin-producer displayed thehighest efficacy reducing the pathogen by 5.54 ± 0.12 and 4.52 ± 0.01 on PTFE and SS, respectively. Industrially,the pre-treatment with L. sakeiCRL1862 or its bacteriocin (exclusion) constitutes the most realistic way toprevent pathogen biofilm settlement. The use of bacteriocins and\/or thebacteriocin-producer strain represents a safe and environmentally friendlysanitation method to mitigate post-processing food contamination.","palabras_clave":"Biofilm|Listeria monocytogenes|Industrial surfaces|Bacteriocin|Lactobacillus sakei CRL1862"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5902,"idioma_id":4,"titulo":"3. Surface tension problems solved with the Particle Finite Element Method using large time-steps","resumen":"In previous works, the authors have presented a highly efficient extension of the Particle Finite Element Method, called PFEM-2,to solve two-phase flows. The methodology which uses X-IVS to treat convection terms allowing large time-steps was validated for problems where the gravity forces and\/or the inertial forces dominate the flow.Although that is the target range of problems to solve with PFEM-2, most of real problems that fall in these categories also includes other flow regimes in certain regions of the domain. Maybe the most common secondary regime is when the surface tension dominates, as an example when drops or bubbles are released from the main flow, and this feature must be taken into account in any complete numerical strategy. Attending to that, in this work the treatment of the surface tension to PFEM-2 is included. An implicit CSF methodology is employed together with a coupling between the marker function with a Level Set function to obtain a smooth representation of the normal of the interface which allows an accurate curvature calculation. Examples for curvature calculation and isolated bubbles and drops are presented where the accuracy and the computational efficiency are analyzed and contrasted with other numerical methodologies. Finally, a simulation of a jet atomization is analyzed. This case presents the above mentioned features: it is a inertia-dominant flow with a surface tension phenomena on drops and ligaments break up that can not be neglected.","palabras_clave":"PFEM|SURFACE TENSION|TWO-PHASE FLOWS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5903,"idioma_id":4,"titulo":"Disrupting effect of androgens in postnatal female domestic cats","resumen":"To test the hypothesis that in domestic cats, postnatal androgens induce sterility, the27 aims of this study were to describe the reproductive effects and the clinical safety of a28 postnatal administration of a long term release androgen in this species. Thirteen newborn29 littermate female kittens were randomly assigned to one of the following treatment groups30 within the first 24 hours of birth: testosterone enanthate 12.5 mg sc (TE; n = 8) or Placebo31 (PL; n = 5). The animals were subsequently assessed for fecal sexual hormones until32 puberty was attained and subsequently when matings occurred. After 21 days, ovulation33 and gestation were diagnosed. All queens were subsequently ovario-hysterectomized. Fecal34 testosterone concentrations differed between the treatment groups throughout the study35 period (P  0.1). While all the females37 were receptive during the pubertal estrus (P> 0.1), two TE (2\/8) compared with all (5\/5)38 females of the PL group had ovulations (P < 0.05). Only one (1\/2) compared with three39 (3\/5) of the queens of the TE and PL groups, respectively became pregnant. All kittens of40 the TE group had transient clitoral enlargement. Anovulatory TE-treated cats had no corpus41 luteum, and a significant diminution of the endometrial glands as well as of the height of42 the uterine epithelium. It is concluded that, in domestic cats, a single postnatal supra43physiological dose of testosterone caused a large proportion of queens to be anovulatory44 and there were also histological endometrial abnormalities that also occ","palabras_clave":"cat|ovulation|disruption|testsosterone"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5904,"idioma_id":4,"titulo":"Improvement of in vitro stability and pharmacokinetics of hIFN-alfa by fusing the carboxyl-terminal peptide of hCG beta\u0003-subunit","resumen":"Improving in vivo half-life and in vitro stability of protein-based therapeutics is a current challenge for the biopharmaceutical industry. In particular, recombinant human interferon alpha-2b (rhIFN-&#945;2b), which belongs to a group of cytokines extensively used for the treatment of viral diseases and cancers, shows a poor stability in solution and an extremely short plasma half-life which determines a strict therapeutic regimen comprising high and repeated doses. In this work, we have used a strategy based on the fusion of the carboxyl-terminal peptide (CTP) of human chorionic gonadotropin (hCG) &#946;-subunit, bearing four O-linked oligosaccharide recognition sites, to each or both N- and C-terminal ends of rhIFN-&#945;2b. Molecules containing from 5 (CTP-IFN and IFN-CTP) to 9 (CTP-IFN-CTP) O-glycosylation sites were efficiently expressed and secreted to CHO cells supernatants, and exhibited antiviral and antiproliferative bioactivities in vitro. Significant improvements in pharmacokinetics in rats were achieved through this approach, since the doubly CTP-modified IFN variant showed a 10-fold longer elimination half-life and a 19-fold decreased plasma apparent clearance compared to the wild-type cytokine. Moreover, CTP-IFN-CTP demonstrated a significant increase in in vitro thermal resistance and a higher stability against plasma protease inactivation, both features attributed to the stabilizing effects of the O-glycans provided by the CTP moiety. These results constitute the first report that postulates CTP as a tag for improving both the in vitro and in vivo stability of rhIFN-&#945;2b which, in turn, would positively influence its in vivo bioactivity.","palabras_clave":"O-Glycosylation|Recombinant human interferon alpha|Carboxyl-terminal peptide|hCG beta\u0003-subunit|Pharmacokinetics|in vitro protein stability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5905,"idioma_id":4,"titulo":"Extra-pair paternity in a population of Chilean Swallows breeding at 54 degrees south","resumen":"Geographic patterns of variation in life-history traits have puzzled researchers for decades.However, the widely accepted idea thatmating systems exhibit a tropical?temperate latitudinal trend, with extra-pair mating systems being the norm among temperate species and genetic monogamy the norm among tropical species, is supported by sparse data, particularly for birds breeding in the tropics and even more so for birds that breed in the southern hemisphere temperate zone. Our objective was to examine the genetic mating system of Chilean Swallows (Tachycineta meyeni) breeding at 54°S in Tierra del Fuego, Argentina. From 2006 to 2009, we examined the paternity of young in 52 broods. Contrary to predictions based on their congeners that breed at equivalent latitudes in the northern hemisphere, Chilean Swallows in our study had low rates of extra-pair paternity (EPP), with 13.5% of nests (N = 52) having at least one extra-pair young and 6.8% of all nestlings (N = 161) fathered by extra-pair males. These rates are also lower than those reported for species of Tachycineta swallows that breed at tropical latitudes. We found no support for a tropical?south temperate latitudinal cline in EPP rates. The highly unpredictable weather of the island of Tierra del Fuego might be influencing parental investment at this site; small clutch sizes and low EPP rates are expected if biparental attention is crucial for chick survival and reproductive success for these aerial insectivores. We argue that the sparse sampling of mating systems in birds worldwide mayhave contributed to a misapprehension of a global pattern. More studies of tropical and south-temperate systems are needed to build on theories based on a wider set of taxa.","palabras_clave":"Mating system|Latitudinal variation|Hirundinidae|life-history traits|Tachycineta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5906,"idioma_id":4,"titulo":"Standardization of molecular monitoring for chronic myeloid leukemia in Latin America using locally produced secondary cellular calibrators","resumen":"Residual disease in chronic myeloid leukemia (CML) patients undergoing therapy with tyrosine kinase inhibitors (TKIs) is measured by assessing the quantity of transcripts of the BCR-ABL1 fusion gene in peripheral white blood cells1. This analysis is based on reverse-transcription quantitative PCR (RT-qPCR) technology; however, the wide array of methods used worldwide has led to large variation in quantitative BCR-ABL1 measurements, which hamper inter-laboratory comparative studies2,3. It is now recognized that monitoring BCR-ABL1\/control gene ratios on the International Scale (IS) is vital for the management of patients with CML4. Efforts to harmonize procedures to measure BCR-ABL1 fusion transcripts have included important investments in sample exchange programs to derive laboratory-specific conversion factors (CF); these efforts showed improvements in inter-laboratory concordance rates, but the process is laborious and limited due to the lack of a common set of reference samples that can be shared on a global scale. This requirement was recently addressed in part by the formulation and validation of the first World Health Organization (WHO) International Genetic Reference Panel for quantitation of BCR-ABL1 by RT-qPCR5. The WHO primary standards consist of a 4-level panel of e14a2-positive lyophilized cell line dilutions. Each level has an assigned IS-value, which was obtained by repeated testing of each sample level in expert IS-standardized laboratories. Unfortunately, the stock of WHO primary standards is limited, and their accessibility has been restricted to manufacturers of testing kits or secondary reference standards. In this study, we aimed to develop and validate secondary reference materials calibrated to the IS through the WHO primary standards in order to facilitate standardization of molecular monitoring in Latin America.","palabras_clave":"RT-QPCR|CML|HARMONIZATION|BCR-ABL1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5907,"idioma_id":4,"titulo":"Autonomous open-source hardware apparatus for quantum key distribution","resumen":"We describe an autonomous, fully functional implementation of the BB84 quantum key distribution protocol using open source hardware microcontrollers for the synchronization, communication, key sifting and real-time key generation diagnostics. The quantum bits are prepared in the polarization of weak optical pulses generated with light emitting diodes, and detected using a sole single-photon counter and a temporally multiplexed scheme. The system generates a shared cryptographic key at a rate of 365 bps, with a raw quantum bit error rate of 2.7%. A detailed description of the peripheral electronics for control, driving and communication between stages is released as supplementary material. The device can be built using simple and reliable hardware and it is presented as an alternative for a practical realization of sophisticated, yet accessible quantum key distribution systems.&nbsp;","palabras_clave":"districucion cuántica de claves|óptica cuántica|comunicaciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5908,"idioma_id":5,"titulo":"Recomendaciones metodológicas para el monitoreo molecular de pacientes con Leucemia Mieloide Crónica por PCR cuantitativa en tiempo real del BCR-ABL1","resumen":"No tiene Abstract","palabras_clave":"LMC|BCR-ABL1|Cuantificación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5909,"idioma_id":4,"titulo":"Argentinean pistachio oil and flour: A potential novel approach of pistachio nut utilization","resumen":"In order to searching a potential novel approach to pistachio utilization, thechemical and nutritional quality of oil and flour from natural, roasted, andsalted roasted pistachios from Argentinian cultivars were evaluated. Thepistachio oil has high contents of oleic and linoleic acid (53.5?55.3, 29?31.4relative abundance, respectively), tocopherols (896?916 &#956;g\/g oil),carotenoids (48?56 &#956;g\/g oil) and chlorophylls (41?70 &#956;g\/g oil), being a goodsource for commercial edible oil production. The processing conditions didnot affect significantly the fatty acid distribution and minor components ofpistachio oil samples. The roasting process not diminish total phenolic (TP)and flavonoids (FL) content significantly compared to natural pistachio flour(NPF), even so reduced the DPPH antioxidant capacity (approximately20 %) in the roasted pistachio flour (RPF). Furthermore, salted roastedpistachio flour (SRPF) showed a slight and significant decrease on TP andFL content in relation to the others samples. The phenolic profile of pistachioflours were evaluated by LC-ESI-QTOF-MS. The major compounds were(+)-catechin (38?65.6 &#956;g\/g PF d.w.), gallic acid (23?36 &#956;g\/g PF d.w.) andcyanidin-3-O-galactoside (21?23 &#956;g\/g PF d.w.). The treatments havedifferent effects on the phenolics constituents of pistachio flour. Roastingcaused a significant reduction of some phenolics, gallic acid and (+)-catechin,and increased others, naringenin and luteolin. Otherwise, salting and roastingof pistachio increased levels of gallic acid and naringenin. These resultssuggest that Argentinian pistachio oil and flour could be considered asingredients into applications that enhance human health.","palabras_clave":"alimentos|pistachio|aceite"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5910,"idioma_id":4,"titulo":"Transcriptional and post-transcriptional control of the plant circadian gene regulatory network","resumen":"The circadian clock drives rhythms in multiple physiological processes allowing plants to anticipate and adjust to periodic changes in environmental conditions. These physiological rhythms are associated with robust oscillations in the expression of thousands of genes linked to the control of photosynthesis, cell elongation, biotic and abiotic stress responses, developmental processes such as flowering, and the clock itself. Given its pervasive effects on plant physiology, it is not surprising that circadian clock genes have played an important role in the domestication of crop plants and in the improvement of crop productivity. Therefore, identifying the principles governing the dynamics of the circadian gene regulatory network in plants could strongly contribute to further speed up crop improvement. Here we provide an historical as well as a current description of our knowledge of the molecular mechanisms underlying circadian rhythms in plants. This work focuses on the transcriptional and post-transcriptional regulatory layers that control the very core of the circadian clock, and some of its complex interactions with signaling pathways that help synchronize plant growth and development to daily and seasonal changes in the environment.","palabras_clave":"CIRCADIAN CLOCK|POST-TRANSCRIPTIONAL REGULATION|NETWORK|TRANCRIPTIONAL REGULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5911,"idioma_id":4,"titulo":"Experimental and AB Initio Study of the hyperfine parameters of ZnFe2O4 with defects","resumen":"We present a combined Mössbauer and ab initio study on the influence of oxygen-vacancies on the hyperfine and magnetic properties of the ZnFe2O4 spinel ferrite. Samples with different degree of oxygen-vacancies were obtained from zinc ferrite powder that was thermally treated at different temperatures up to 650 oC under vacuum.Theoretical calculations of the hyperfine parameters, magnetic moments and magnetic alignment have been carried out considering different defects such as oxygen-vacancies and cation inversion. We show how theoretical and experimental approaches are complementary to characterize the local structure around Fe atoms and interpret the observed changes in the hyperfine parameters as the level of defects increases","palabras_clave":"Ferrites|Zn Ferrite|Hyperfine Interactions|ZnFe2O4"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5912,"idioma_id":4,"titulo":"Effects of Nanostructure and Dipolar Interactions on Magnetohyperthermia in Iron Oxide Nanoparticles","resumen":"Magnetohyperthermia properties of magnetic nanoparticle colloids are strongly affected by their intrinsic magnetic properties and dipolar interactions among themselves. The intrinsic magnetic properties are related to the nanoparticle (NP) size, geometry, phase composition, magnetic anisotropy, and saturation magnetization. The dipole?dipole interactions are determined by colloid nanoparticle concentrations and the possible existence of clustering on the colloidal suspension. Here we have observed that oxygen atmosphere and pressure changes during the final stage of thermal decomposition are critical to modify the size of the iron oxide NPs from 8 to near 20 nm, and consequently their overall magnetic properties. Size-dependent magnetic parameters such as anisotropy, magnetic moment per particle, blocking temperature, and dipolar interaction energy were inferred using different phenomenological approaches. A detailed magnetohyperthermia analysis was performed by applying the linear response theory. A good correlation between experimental and theoretical specific absorption rate values was obtained for a frequency of 260 kHz and applied field of 52 kA\/m. These results were observed for the different sizes of nanoparticles, and disagreement between the experimental results and the model increases at lower frequencies.","palabras_clave":"hyperthermia|SAXS|dipolar interactions|magnetic nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5913,"idioma_id":4,"titulo":"Surface magnetic contribution in zinc ferrite thin films studied by element- and site-specific XMCD hysteresis-loops","resumen":"Element- and site-specific magnetic hysteresis-loops measurements on a zinc ferrite (ZnFe2O4) thin film were performed by X-ray magnetic circular dichroism. Results show that iron in octahedral and tetrahedral sites of spinel structure are coupled antiferromagnetically between them, and when magnetic field is applied the magnetic moment of the ion located at octahedral sites aligns along the field direction. The magnetic measurements reveal a distinctive response of the surface with in-plane anisotropy and an effective anisotropy constant value of 12.6 kJ\/m3. This effective anisotropy is due to the combining effects of demagnetizing field and, volume and surface magnetic anisotropies KV = 3.1 kJ\/m^3 and KS = 16 &#956;J\/m^2.","palabras_clave":"Maghemite nanoparticles|Zinc ferrite|XMCD hysteresis-loops|Surface anisotropy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5914,"idioma_id":4,"titulo":"Contextual predictability enhances reading performance in patients with schizophrenia","resumen":"In the present work we analyzed fixation duration in 40 healthy individuals and 18 patients with chronic, stable SZ during reading of regular sentences and proverbs. While they read, their eye movements were recorded. We used lineal mixed models to analyze fixation durations. The predictability of words N&#8722;1, N, and N+1 exerted a strong influence on controls and SZ patients. The influence of the predictabilities of preceding, current, and upcoming words on SZ was clearly reduced for proverbs in comparison to regular sentences. Both controls and SZ readers were able to use highly predictable fixated words for an easier reading. Our results suggest that SZ readers might compensate attentional and working memory deficiencies by using stored information of familiar texts for enhancing their reading performance. The predictabilities of words in proverbs serve as task-appropriate cues that are used by SZ readers. To the best of our knowledge, this is the first study using eyetracking for measuring how patients with SZ process well-defined words embedded in regular sentences and proverbs. Evaluation of the resulting changes in fixation durations might provide a useful tool for understanding how SZ patients could enhance their reading performance.","palabras_clave":"Modealdo|Cognición|Esquizofrenia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5915,"idioma_id":4,"titulo":"Word processing during reading sentences in patients with schizophrenia: evidences from the eyetracking technique","resumen":"Purpose: The current study analyze the effect of word properties (i.e., word length, word frequency and word predictability) on the eyemovement behavior of patients with schizophrenia (SZ) compared to age-matched controls.Method: 18 SZ patients and 40 age matched controls participated in the study. Eye movements were recorded during reading regularsentences by using the eyetracking technique. Eye movement analyses were performed using linear mixed models.Findings: Analysis of eye movements revealed that patients with SZ decreased the amount of single fixations, increased their total number of second pass fixations compared with healthy individuals (Controls). In addition, SZ patients showed an increase in gaze duration, compared to Controls. Interestingly, the effects of current word frequency and current word length processing were similar in Controls and SZ patients. The high rate of second pass fixations and its low rate in single fixation might reveal impairments in working memory when integrating neighbor words. In contrast, word frequency and length processing might require less complex mechanisms, which were functioning in SZ patients. Conclusion: To the best of our knowledge, this is the first study measuring how patients with SZ process dynamically well-defined wordsembedded in regular sentences. The findings suggest that evaluation of the resulting changes in eye movement behavior may supplement current symptom-based diagnosis.","palabras_clave":"Eye movement modeling |Cognition|Squizofrenia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5916,"idioma_id":4,"titulo":"Males choose to keep their heads: Preference for lower risk females in a praying mantid","resumen":"Male reproductive success is obviously mate limited, which implies that males should rarely be choosy. One extreme case of a reproductive (or mating) cost is sexual cannibalism. Recent research has proposed that male mantids (Parastagmatoptera tessellata) are choosy and not complicit in cannibalism and that they modify behavior towards females based on the risk imposed by them. Since female cannibalism depends on females? energetic state (i.e. hunger) we investigated whether male mantids are capable of using environmental cues that provide information regarding the energetic state of females to make their mate choices. Under laboratory conditions, males were confronted individually with three options: a female eating a prey, a female without a prey, and a male eating a prey (as a control for the presence of prey). Each subject comprising a choice was harnessed and placed in the corners of a triangular experimental arena at an equidistant distance from the focal male. The prey was a middle size cricket that subjects ate in approximately twenty minutes. The behavior of focal males was recorded for six hours. Females were under the same deprivation regime and, in line with previous studies, consuming one cricket did not significantly increase females? abdomen girth. Male mantids significantly preferred females that were eating a prey. In all cases choices were made after the females consumed the whole prey. This suggests that males did not use the prey as a direct way to avoid being cannibalized by keeping the female busy. The preference for females that had recently fed may have evolved because of the potential reduction in sexual cannibalism.","palabras_clave":"canibalismo sexual|eleccion de pareja|mantidos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5917,"idioma_id":5,"titulo":"Miradas e instrumentos para la catalogación de paisajes latinoamericanos. Perspectivas emergentes.","resumen":"El trabajo explora y discute herramientas teóricas e instrumentales asociadas a la producción de paisajes latinoamericanos desde la perspectiva del trabajo en red. El análisis se enfoca en tensionar los actuales instrumentos derivados de las normativas internacionales con argumentos centrados en elementos y dimensiones que los explican y dan sentido. Desde una mirada que pone énfasis en las relaciones sociales como agente territorializador, se describen las nociones de paisaje subyacentes, así como las perspectivas teóricas que sostienen algunas de las instrumentaciones para aprehender y reconocer qué paisajes hemos producido y estamos produciendo socialmente. Más que presentar un trabajo acabado en sí mismo, se trata de compartir el proceso de algunas líneas de trabajo que convergen en el seno de un grupo de investigación en formación, la Red Interuniversitaria de Paisajes Latinoamericanos (RIPLa). En primer lugar, se analizan los documentos internacionales que guían el debate en los procesos de construcción de instrumentos en el contexto latinoamericano, para luego exponer tres ejes argumentales que anidan en la RIPLA y que aportan miradas diferenciales hacia el paisaje. Finalmente se reconocen acuerdos, discrepancias desafíos y oportunidades para la producción de conocimiento en el marco del trabajo en red.","palabras_clave":"Paisajes Latinoamericanos|Redes de Conocimiento|Instrumentos|Perspectivas Emergentes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5918,"idioma_id":4,"titulo":"Pronase hydrolysis as a pretreatment for quantifying Maillard intermediates during toasting of cornflakes","resumen":"Some of the products generated by the Maillard reaction are desired and very important for dening consumer acceptance of breakfast cereals. However, in recent years there has been an increased concern about compounds that are potentially harmful such as furfurals. The aim of this work was to analyze the eectiveness of protein hydrolysis with pronase as a pretreament for the evaluation of furfurals generated by the Maillard reaction during toasting of corn akes and the parallel development of brown and fuorescent compounds. Furfurals were more accurately quantied with the pronase hydrolysis pretreatment because the protein matrix binds furfurals and fluorescent compounds. For control of the early reaction steps in the toasting process, the most sensitive parameter was fluorescence.","palabras_clave":"Maillard|Furfural|Hydroxymethylfurfural|Fluorescence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5919,"idioma_id":4,"titulo":"Efficient Simulation of Hybrid Renewable Energy Systems","resumen":"This article explores the usage of novel tools for realistic modeling and efficient simulation of Hybrid Renewable Energy Systems (HRES). Using the object oriented Modelica language, a new library providing component models such as photovoltaic (PV) cells, proton exchange membrane (PEM) fuel cells, electrolyzers, hydrogen storage tanks, batteries and electronic converters is developed and used to build different HRES models. Since the components are represented under realistic assumptions, the resulting models exhibit frequent discontinuities, strong non-linearities and combinations of slow and fast dynamics (i.e. stiffness). As these features impose severe limitations to classic numerical simulation solvers, we analyze the use of a new family of numerical algorithms called Quantized State Systems (QSS) that overcome most of those difficulties. The results obtained show that these algorithms applied to realistic HRES are more than one order of magnitude faster than the most efficient classic solvers,&nbsp; allowing to simulate these systems in reasonable times.&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;","palabras_clave":"Hybrid renewable energy systems|Modelica|Quantized State System Simulation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5921,"idioma_id":4,"titulo":"Fracture behavior of polycaprolactone\/ clay nanocomposites","resumen":"The effect of clay-organo modifier on the thermal and mechanical properties and fracture behaviors of pure polycaprolactone (PCL) and 5 wt% PCL\/clay nanocomposites were studied. The different materials were prepared by melt intercalation. It was demonstrated by X-ray diffractometry, differential scanning calorimetry, tensile, and fracture tests that the addition of modified nanoclays affected significantly the final properties of the materials. The optimal combination of properties was achieved with the PCL reinforced with 5 wt% of C30B obtaining improvements of 17% in the Young´s modulus and 1500% in the specific essential fracture work.","palabras_clave":"BIODEGRADABLE POLYMER|CLAY|FRACTURE|NANOCOMPOSITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5922,"idioma_id":4,"titulo":"Immunomodulation of Lactobacillus reuteri CRL1324 on Group B Streptococcus vaginal colonization in a murine experimental model","resumen":"Problem: Maternal Group B Streptococcus (GBS) colonization is a risk factor for infectious disease in newborns. One promising strategy is the modulation of vaginal defense to increase the host?s ability to combat infection. Methods of study: The effect of intravaginal (i.va.) Lactobacillus reuteri CRL1324 inoculation on different immune cell populations, cytokines and immunoglobulin isotypes in a murine model of GBS vaginal colonization was evaluated Results: Seven i.va. inoculations of L. reuteri CRL1324 previous to GBS challenge showed an immunomodulatory effect on the cells and mediators of innate immunity, decreasing the number of neutrophils induced by the pathogen and increasing the activated macrophage population. Moreover, increases of B-lymphocytes and IgA and IgG subclasses were observed in mice inoculated with L. reuteri CRL1324 and then challenge with GBS challenge. Conclusion: L. reuteri CRL1324 shows a protective effect against GBS colonization that could be mediated by the modulation of the immune response.","palabras_clave":"GROUP B STREPTOCOCCI|LACTOBACILLUS|VAGINAL TRACT|PREVENTIVE EFFECT|IMMUNE CELLS|MICE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5923,"idioma_id":4,"titulo":"Behavioral swimming effects and acetylcholinesterase activity changes in Jenynsia multidentata exposed to chlorpyrifos and cypermethr in individually and in mixtures","resumen":"The pesticides cypermethrin (CYP) and chlorpyrifos (CPF) were found together in water bodies located in agricultural and urban areas. However, the impact to non-target biota from exposure to mixtures has received little attention. In the current study, we evaluated changes in swimming behavior and cholinesterase enzymes activity in Jenynsia multidentata, to investigate the possible effects of these insecticides individually and in mixtures. Moreover, differences between technical and commercial mixtures of the pesticides were evaluated. Females of J. multidentata were exposed over 96 h to CYP (0.04 and 0.4 µg L-1), CPF (0.4 and 4 µg L-1), individually and in a technical and commercial mixtures. Swimming behavior was recorded after 24 h and 96 h of exposure. Also, we measured cholinesterase enzymes activity in brain and muscle after 96 h of exposure. Exposure to CYP increased the exploratory activity of J. multidentata in the upper area of the aquarium. Fish exposed to CPF (4 µg L-1) showed a decrease in swimming activity and an increase in the time spent at the bottom of the aquarium. Interestingly, fish exposed to the technical and commercial mixture of CYP and CPF displayed a different behavior based on the concentration of exposure. Low concentration of pesticides elicited an increase in J. multidentata swimming activity with preference for the upper area of the aquarium, and high concentrations caused decrease in swimming activity with preference for the bottom area of the aquarium. Based on the response of cholinesterase enzymes, acetylcholinesterase in muscle was more sensitive to exposure to CYP, CPF and their mixtures than in brain. A decrease in swimming behavior correlates significantly with the inhibition of acetylcholinesterase activity in muscle of J. multidentata exposed to high concentrations of pesticides. These results draw attention to the need of more studies on the potential ecotoxicological impact of pesticides and its mixtures at environmental relevant concentrations.","palabras_clave":"Fish behavior|Acetylcholinesterase activity|Mixtures|Insecticides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5924,"idioma_id":4,"titulo":"Bioaccumulation and biochemical response in South American native species exposed to Zinc: Boosted Regression Trees as novel tool for biomarkers selection","resumen":"The aim of this study was to evaluate the response of a wide battery of biomarkers in two native species,the freshwater shrimp Palaemonetes argentinus and the macrophyte Potamogeton pusillus, experimen-tally exposed to zinc in order to establish the potential use of selected species as bioindicators of aquaticpollution. For this purpose, we propose the use of integrated biomarker index (IBR) with a previous selec-tion of biomarkers using boosted regression trees (BRTs) as a new tool in ecotoxicology. Organisms werecollected from a reference site, acclimated and exposed at relevant environmental zinc levels (control, 5,50 and 500 g Zn L&#8722;1) for 96 h. Biomarkers were measured in cephalothorax and abdomen of shrimp aswell as in leaf, stem and root of plants.Significant zinc accumulation was observed in cephalothorax of P. argentinus from 50 g Zn L&#8722;1andfrom 5 g Zn L&#8722;1in stem and root of P. pusillus, when compared with control condition. Those effectbiomarkers with significant differences among treatments were pre-selected to run out the BRTs modelfor each species. In P. argentinus, microsomal acetylcholinesterase activity, metallothioneins and superox-ide dismutase activity measured in cephalothorax, as well as glutathione reductase activity in abdomen,showed the higher capacity to explain or predict the zinc exposure concentration. On the contrary, in P.pusillus, only chlorophyll a measured in leaf and H2O2measured in root were the more representative ofexposure concentrations, at least, within the biomarkers tested in the present study. Thereafter, IBR wascalculated with the selected biomarkers in P. argentinus and showed in a sole value the organism stress,which also correlates with zinc exposure and accumulation.Natives species tested displayed a sensitive response to metal exposure, which represents an importantcharacteristic for biomonitoring programs. Our findings suggest that the BRTs and IBR are useful androbust run tools to select the better biomarkers in toxicological studies and indicate the organism stress.","palabras_clave":"South American species|Biomarkers|Bioindicators|Aquatic contamination|Boosted regression trees"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5925,"idioma_id":4,"titulo":"New circumscription of the genus Gamochaeta (Asteraceae, Gnaphalieae) inferred from Nuclear and Plastid DNA Sequences","resumen":"Gamochaeta (tribe Gnaphalieae, Asteraceae) is composed of ca. 60 species primarily distributed in tropical and subtropical America. Within the tribe Gnaphalieae, the genus is characterized by capitula arranged in spikes or head-like clusters, few hermaphroditic central florets, truncate style branches with apical sweeping trichomes, pappus bristles connate at the base into a ring falling as a unit, and achenes with globose twin trichomes. Previous molecular phylogenetic studies have suggested the paraphyly of the genus, but have not provided a basis for redefining generic limits due to incomplete taxon sampling. To address this problem, DNA sequences from the plastid (trnL-F) and nuclear (ETS and ITS) genomes were analyzed from a broad taxon sample representing the full range of morphological variation known in the genus. Our results affirm that Gamochaeta is paraphyletic as presently circumscribed. Two clades can be recognized: one clade that includes the majority of the species currently assigned to Gamochaeta and a second clade that includes Gamochaetopsis, Stuckertiella and seven species of Gamochaeta. We present here a new circumscription of Gamochaeta, including two new combinations, Gamochaeta alpina and Gamochaeta peregrina, and the resurrection of Gamochaeta capitata. Our results also show Omalotheca supina, O. norvegica and O. sylvatica, which were placed by some authors in Gamochaeta or in Gnaphalium, form a monophyletic group distantly related to both genera.","palabras_clave":"GAMOCHAETOPSIS|STURCKETIELLA|OMALOTHECA|ITS ETS TRNL-F"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5926,"idioma_id":4,"titulo":"Diversity of freshwater ciliates (Protista) from Argentina","resumen":"Diversity of ciliates from freshwater and soil were scarcely investigated in Argentina, in spite of their ecological role in these ecosystems and the huge environmental heterogeneity that can be found in this country. In the present study, we describe the morphology of nine species from a temporary pond in Buenos Aires province, by means of live observations and protargol impregnations. Stentor igneus Ehrenberg, Pseudochilodonopsis piscatoris (Blochmann), Vorticella halophila Stiller, Intranstylum invaginatum Stokes, and Epistylis rotans ?vec were recorded for the first time in Argentina, and in most cases, are new for the Neotropical realm as well. In addition, an updated checklist on freshwater ciliates from Argentina is provided, based on ciliates listed for the Salado River basin in the Buenos Aires province, new findings from Rancho Hambre peat bog pools in Tierra del Fuego province and a pond from Misiones province.","palabras_clave":"ARGENTINA|CILIOPHORA|DIVERSITY|NEW RECORDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5927,"idioma_id":4,"titulo":"Transcriptome modulation during host shift is driven by secondary metabolites in desert Drosophila","resumen":"High-throughput transcriptome studies are breaking new ground to investigate theresponses that organisms deploy in alternative environments. Nevertheless, muchremains to be understood about the genetic basis of host plant adaptation. Here, weinvestigate genome-wide expression in the fly Drosophila buzzatii raised in different conditions. This species uses decaying tissues of cactus of the genus Opuntia as primary rearing substrate and secondarily, the necrotic tissues of the columnar cactus Trichocereus terscheckii. The latter constitutes a harmful host, rich in mescaline and other related phenylethylamine alkaloids. We assessed the transcriptomic responses of larvae reared in Opuntia sulphurea and T. terscheckii, with and without the addition of alkaloids extracted from the latter. Whole-genome expression profiles were massively modulated by the rearing environment, mainly by the presence of T. terscheckii alkaloids. Differentially expressed genes were mainly related to detoxification, oxidation\/reduction and stress response; however, we also found genes involved in development and neurobiological processes. In conclusion, our study contributes new data onto the role of transcriptional plasticity in response to alternative rearing environments.","palabras_clave":"RNA-seq|mescaline|alkaloids|environmental adaptation|plasticity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5928,"idioma_id":5,"titulo":"Método de Medición de la Percepción de los usuarios sobre los sistemas E-Learning de los Centros e Instituciones Educativas","resumen":"Es importante que los usuarios\/alumnos de un sistema E-learning se sientan satisfechos con el entorno yla interfaz. Estos entornos deberían contar con métodos de evaluación de calidad y mejora. Del mismo modo se hacenecesario garantizar que las medidas y los valores de los indicadores sean repetibles y comparables entre las diferentesinstancias de evaluación de la calidad.En este trabajo se propone un método de Medición de Calidad, en el dominio de E-Learning, para proveer una medidaparcial tanto en la Usabilidad (QE) como en la Satisfacción (QinU) que permita evaluar y, en el futuro, poder mejorar lacalidad en las mismas.Este método de detección de la percepción de los usuarios arrojará una medida parcial de Usabilidad y Satisfacción, debidoa que en este trabajo no están consideradas todas las sub características indicadas en la ISO 25010 y en el modelo 2Q2U,sino mas bien solo se consideran las características de índole subjetiva que permitan la evaluación por medio decuestionarios o entrevistas con el usuario.Como resultado de la Medición y Evaluación se especificarán requerimientos No Funcionales de aspectos de unaaplicación E-Learning, particularmente atributos de Atractividad y Estética de la Interfaz, en cuanto a Usabilidad, y Placery Confort en cuanto a Satisfacción.","palabras_clave":"Centros Educativos, Calidad, Evaluación, Marco INCAMI, Modelo 2Q2U, Cuestionarios."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5929,"idioma_id":4,"titulo":"Motor features in Parkinson's disease with normal olfactory function","resumen":"BACKGROUND:Normosmic Parkinson´s disease (PD) might be a unique clinical phenotype with a more benign course when compared with hyposmic PD.OBJECTIVE:The objective of this study was to evaluate motor features and the acute levodopa response according to olfactory function.METHODS:A total of 169 de novo PD patients that underwent olfactory testing and acute levodopa challenge for clinical prediction of sustained long-term dopaminergic response were evaluated.RESULTS:The overall frequency of normosmia was 33%. Normosmic PD patients scored nonsignificantly different to hyposmic\/anosmic patients on motor scale and on degree of improvement with levodopa. Motor scores at follow-up were comparable among groups.CONCLUSIONS:Normal olfactory function is common in early PD and was not associated with a different motor phenotype when compared with PD patients with olfactory dysfunction. © 2016 International Parkinson and Movement Disorder Society.","palabras_clave":"PARKINSON|OLFACTORY FUNCTION|MOTOR FEATURES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5930,"idioma_id":4,"titulo":"Influence of white matter MRI hyper-intensities on acute l-dopa response in patients with Parkinson's disease","resumen":"OBJECTIVE:To determine whether brain white matter hyperintensities (WMH) influence l-dopa response in Parkinson´s disease (PD) patients.METHODS:We prospectively evaluated 60 PD patients with an acute l-dopa challenge test, and assessed motor performance with the Movement Disorders Society revised Unified Parkinson´s Disease Rating Scale (MDS-UPDRS) during 'ON' and 'OFF' medication states. Magnetic resonance images were examined using a visual semi-quantitative rating scale for quantification and distribution analysis of WMH. l-dopa challenge test response was correlated to extent and location of WMH, to determine a potential association between them.RESULTS:Subjects with greater deep WMH burden, showed less response to l-dopa on axial motor symptoms (R = -0.35; p < 0.027), when tested with Part III of the MDS-UPDRS before and after acute levodopa challenge.CONCLUSIONS:Results suggest WMH may affect response to l-dopa on axial function of PD patients, which could be due to either non-dopaminergic (cortico-basal ganglia) motor pathway disruption, or postsynaptic nigrostriatal pathway involvement.","palabras_clave":"WHITE MATTER|PARKINSON|MRI|L-DOPA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5931,"idioma_id":4,"titulo":"Comparison of Olfactory Identification Patterns among Parkinson's Disease Patients from Different Countries","resumen":"Olfactory function assessment is an important screening tool and also may differentiate Parkinson´s disease (PD) patients from other parkinsonisms, including nondegenerative ones, such as, normal pressure hydrocephalus, vascular, drug induced, or infectious parkinsonism. Several authors in different countries have reported various sets of odors that best differentiate between these conditions. It is debated if distinctive patterns of 'restrictive' or 'selective' hyposmia in PD may be affected by cultural aspects. To compare the olfactory identification function in PD across different countries, we analyzed Sniffin´ Sticks identification task results between 112 PD patients from Argentina and previously reported data of PD patients from Brazil (106 patients), the Netherlands (400 patients), Germany (40 patients), China (110 patients), and Sri Lanka (89 patients). Categorical principal component analysis (CATPCA) was performed to find components reflecting groups of odors similarly perceived across subjects. CATPCA analysis found 2 components for each group which shared 10 out of 16 odors amongst each other. We found that only the shared items of component 2 (orange, mint, banana, garlic, coffee, cloves, and fish) showed uniform results across all of the included countries, whereas variations in component 1 (licorice, turpentine, and apple) were attributed mostly to differences across control groups.","palabras_clave":"OLFACTORY|PARKINSON|EPIDEMIOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5932,"idioma_id":4,"titulo":"A novel method for cardiac vector velocity measurement: Evaluation in myocardial infarction.","resumen":"Background and objective: Pathological alterations provoked by myocardial infarction cause slow conduc-tion by increasing axial resistance on coupling between cells. This issue may cause abnormal patterns inthe dynamics of the tip of the cardiac vector. Methods: In this work, we have developed a method to compute the angular velocity during ventricularrepolarization from Frank XYZ leads, using the concept of quaternion. This parameter jointly with thelinear velocity obtained by differentiation and the spatial velocity reported by others during ventriculardepolarization, have been combined in order to design a myocardial infarction detector (so-called indexof cardiac vector velocity: ICVV) with high values of sensitivity and specificity simultaneously. Results: The predictive power of ICVV has been tested in two groups: patients with less than 7 daysafter infarction, achieving 98% of sensitivity and 97% of specificity; and patients with more than 45 daysafter infarction, achieving 92% of sensitivity without loss of specificity. The former group is importantfor early detection of myocardial infarction and begins treatment in a short period of time on emergencydepartment. The latter involves the evaluation of the cardiac vector velocity after the period of post-infarction electrical remodeling which may be useful in the follow-up of patients. Conclusions: We have concluded that this method extends the concept of cardiac vector velocity and maybe useful in the diagnosis of myocardial infarction.","palabras_clave":"VCG|MYOCARDIAL|INFARCTION|CARDIAC|VECTOR|VELOCITY|QUATERNION|ICVV"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5933,"idioma_id":4,"titulo":"Learning in Vespula germanica social wasps: situations of unpredictable food locations","resumen":"Vespula germanica is a social wasp that frequently forages on carrion, making several trips between un-depleted resources and the nest. In the present study, we analyze this food relocation behavior, evaluating V. germanica response in situations of successive changes in food location. To this end, we allowed individual wasps to feed five consecutive times from a dish located within an array of landmarks. After each return, the feeder was randomly displaced 60 cm from the previous location, so that when the wasp retuned to the array it encountered a different scenario. We found that on their first return to the new situation, wasps first went to the previously learned location and took some time to find the displaced feeder. Interestingly, during the following returns, wasps discovered the novel food location more rapidly, even though its location randomly changed at each return. Thus, after the first experience of food absence, wasps seem to have learned that food would not necessarily be present at the previous site. Free-flying wasps use past experience of food position change to reduce searching time at previously learned locations. These findings illustrate wasp behavioral plasticity in uncertain foraging contexts.","palabras_clave":"FORAGING BEHAVIOR|UNPREDICTABLE CONTEXTS|LEARNING|SOCIAL WASPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5934,"idioma_id":4,"titulo":"Long-term spatial memory in Vespula germanica social wasps: the influence of past experience on foraging behavior","resumen":"Social insects exhibit complex learning and memory mechanisms while foraging. Vespula germanica (Fab.) (Hymenoptera: Vespidae) is an invasive social wasp that frequently forages on un-depleted food sources, making several trips between the resource and the nest. Previous studies have shown that during this relocating behavior, wasps learn to associate food with a certain site, and can recall this association 1 hour later. In this work, we evaluated whether this wasp is capable of retrieving an established association after 24 hours. To this end, we trained free flying wasps to collect food from a certain experimental array, with 2, 4 or 8 feeding trials. After the training phase, the array was removed and set up again 24 hours later, but this time a second baited plate was placed opposite the first. After 24 hours we recorded the rate of wasps that returned to the experimental area and those which collected food from the previously learned feeding station or the non-learned one. During the testing phase, we observed that a low rate of wasps trained with 2 collecting visits returned to the experimental area (22%)  whereas, the rate of returning wasps trained with 4 or 8 collecting visits was higher (51% and 41%, respectively).  Moreover, wasps trained with 8 feeding visits collected food from the previously learned feeding station in a higher rate than those that did from the non-learned one. In contrast, wasps trained 2 or 4 times chose both feeding stations in a similar rate. Thus, significantly more wasps returned to the correct feeding station after 8 repeated trips, but not after only 2 or 4 visits. This is the first research that demonstrates the existence of long-term memory in V. germanica wasps, contributing to further understanding of cognitive mechanisms in social insects.","palabras_clave":"LEARNING|FORAGING BEHAVIOR|MEMORY|SOCIAL WASPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5935,"idioma_id":4,"titulo":"Species of Iothia (Gastropoda: Lepetidae) from shallow waters of the Magellan Region","resumen":"The Lepetidae are currently assumed to be represented by only one valid species in the&nbsp; Magellan region: Iothia emarginuloides (Philippi, 1868), of which Tectura (Pilidium) coppingeri Smith, 1881 and Iothia coppingeri magellanica Linse, 2002 were regarded as synonyms. The extant records of this species are scarce,although they suggest a wide distributional range in the Pacific Ocean, from off Concepción (36ºS) to the Beagle Channel (55ºS), and a restricted distribution inthe Atlantic Ocean, where the species is only known from the Malvinas \/ Falkland Islands and Isla de los Estados (about 54.5ºS). This study provides new information on the occurrence of the genus Iothia in the Magellan region, including morphological, anatomical and molecular evidence. The synonymy of Tectura (Pilidium) coppingeri, Iothia coppingeri magellanica and Iothia emarginuloides is ratified. The species is properly redescribed and figured, and its intraspecific variability discussed, particularly regarding its relation with depths. Furthermore, the distribution of this species in the southwestern Atlantic is extended north up to San Matías Gulf (about 41ºS). Iothia megalodon Warén, Nakano &amp; Sellanes, 2011, previously known from deep waters off Concepción (36ºS), is here reported for the first time for the Magellan region. Its finding in shallow waters from the Beagle Channel greatly extends its distribution both latitudinally and bathymetrically.","palabras_clave":"SOUTH AMERICA|PATELLOGASTROPODA|LIMPET|DIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5936,"idioma_id":4,"titulo":"Liposoluble vitamins in Crustaceans Feed: Metabolic and histological responses","resumen":"Vitamins are vital for normal growth and survival of living organisms and they are distributed in feedstuffs in small quantities. This review is focused on the liposoluble vitamins (A, D, E and K) in the diets and metabolic responses of the Argentine penaeoid shrimps Pleoticus muelleri and Artemesia longinaris, distributed along the South American coast line. Growth, survival and histological analyses serve as indicators of the nutritional value derived from vitamin deficiency. Liposoluble vitamins are also related to stress, antioxidant defense and immune response of shrimps. Effective diet for shrimp culture that provide not only macronutrients including protein and lipid but also micronutrients such as vitamins for optimal growth is an ever improving subject. This review may help formulating suitable feeds for shrimps.","palabras_clave":"vitamins|crustacean|feed|responses"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5937,"idioma_id":4,"titulo":"Unravelling the identity of Pandora species (Bivalvia: Pandoridae) from Southern South America","resumen":"Four species of Pandora have been reported from southern South America: P. cistula Gould, 1850, P. braziliensis G. B. Sowerby II, 1874, P. diffissa Mabille & Rochebrune, 1889 and P. patagonica (Dall, 1915). The group has received little taxonomic attention in this area, resulting in arbitrary and wrong usage of these names, for which several contradictory synonymies have been proposed. This study provides the first revision of Pandora species living in southern South America, including photographs of the type material and descriptions of shells, gross anatomy and living animals. Out of the four species previously mentioned, P. braziliensis is here regarded as valid, including P. patagonica and P. diffissa as synonyms. The fourth species, P. cistula, remains known only from its type specimen. In addition, a new species, P. brevirostris, is described from the shallow waters of Argentina.","palabras_clave":"BIVALVIA|PANDOROIDEA|BIODIVERSITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5938,"idioma_id":4,"titulo":"Magnetised Accretion Discs in Kerr Spacetimes II: Hot Spots","resumen":"Context. Quasi-periodic variability has been observed in a number of X-ray binaries that harbor black hole candidates. In general rela-tivity, black holes are uniquely described by the Kerr metric and, according to the cosmic censorship conjecture, curvature singularitiesalways have to be clothed by an event horizon.Aims. In this paper, we study the observed light curves that arise from orbiting hotspots in thin accretion discs around Kerr blackholes and naked singularities, and the effect introduced by the presence of an external magnetic field.Methods. We employ a ray-tracing algorithm to calculate the light curves and power spectra of these hot spots as seen by a distantobserver for uniform and dipolar magnetic field configurations, assuming a weak coupling between the magnetic field and the discmatter.Results. We show that the presence of an external dipolar magnetic field leads to potentially observable modifications of theselight curves for both Kerr black holes and naked singularities, while an external uniform magnetic field has practically no effect.In particular, we demonstrate that the emission from a hotspot, which is orbiting near the innermost stable circular orbit of a nakedsingularity in a dipolar magnetic field, can be significantly harder than the emission of the same hotspot in the absence of this type ofmagnetic field.Conclusions. The comparison of our model with observational data may allow us to study the geometry of magnetic fields aroundcompact objects and to test the cosmic censorship conjecture in conjunction with other observables, such as thermal continuum spectraand iron line profiles.","palabras_clave":"Black hole physics|Accretion, accretion discs|Magnetic fields|Gravitation|X-rays: binaries|Relativistic processes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5939,"idioma_id":4,"titulo":"Detailed study of SNR G306.3 - 0.9 using XMM-Newton and Chandra observations","resumen":"Aims. We aim to study the spatial distribution of the physical and chemical properties of the X-ray emitting plasma of the supernovaremnant (SNR) G306.3&#8722;0.9 in detail to obtain constraints on its ionization stage, the progenitor supernova explosion, and the age ofthe remnant.Methods. We used combined data from XMM-Newton and Chandra observatories to study the X-ray morphology of G306.3&#8722;0.9 in detail. A spatially resolved spectral analysis was used to obtain physical and geometrical parameters of different regions of the remnant. Spitzer infrared observations, available in the archive, were also used to constrain the progenitor supernova and study the environment in which the remnant evolved.Results. The X-ray morphology of the remnant displays a non-uniform structure of semi-circular appearance, with a bright southwest region and very weak or almost negligible X-ray emission in its northern part. These results indicate that the remnant is propagating in a non-uniform environment as the shock fronts are encountering a high-density medium, where enhanced infrared emission is detected. The X-ray spectral analysis of the selected regions shows distinct emission-line features of several metal elements, confirming the thermal origin of the emission. The X-ray spectra are well represented by a combination of two absorbed thermal plasma models: one in equilibrium ionization (VAPEC) with a mean temperature of &#8764;0.19 keV, and another out of equilibrium ionization (VNEI) at a higher temperature of &#8764;1.1 or 1.6?1.9 keV. For regions located in the northeast, central, and southwest part of the SNR, wefound elevated abundances of Si, S, Ar, Ca, and Fe, typical of ejecta material. The outer regions located northwest and south show values of the abundances above solar but lower than to those found in the central regions. This suggests that the composition of the emitting outer parts of the SNR is a combination of ejecta and shocked material of the interstellar medium. The comparison between the S\/Si, Ar\/Si, and Ca\/Si abundances ratios (1.75, 1.27, and 2.72 in the central region,  respectively), favor a Type Ia progenitor for this remnant, a result that is also supported by an independent morphological analysis using the X-ray and 24 µm IR data.","palabras_clave":"ISM: individual objects: G306.3-0.9|ISM: supernova remnants|X-ray: ISM|radiation mechanism: thermal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5940,"idioma_id":4,"titulo":"Feeding strategies of a small mammal (Phyllotis xanthopygus, Rodentia Cricetidae) at diverse altitudes in the Central Andes, Argentina","resumen":"Knowledge of feeding habits not only contributes information related to theresources that individuals need to survive and breed, but also leads to insights about the interactions between a species and its environment. The optimal foraging theory explains diet selection by several attributes of the trophic scenario such as availability, diversity, quality and predictability of food. Other models propose that digestive tract mass increases under high metabolic demands by cold, affecting food choice and intake. Thus, diet selection emerges as a behavioural trait shaped by intrinsic and extrinsic factors. The goal of our study was to determine variation in the trophic scenario among sites at different elevations, as well as variation in phenotypic traits relevant to the nutritional and energy balance in Phyllotis xanthopygus. This small rodent is widely distributed along the Andes Mountains. We assessed diet selection and digestive tract size in individuals collected at three elevations across its distribution range. Results on dietary proportion of specific trophic categories (green parts, fruits and arthropods) showed that P. xanthopygus alternates between omnivory and granivory\/frugivory. Richness, diversity and quality of the available resources evidenced a relatively low-quality trophic scenario at high altitude. Nevertheless, the diets built in by animals from diverse altitudes lacked differentiation in quality or diversity. P. xanthopygus seems to behaviourally compensate environmental variation to cope with nutritional requirements, by changing diet composition and proportion of items included. The resultant uniform diet quality is consistent with the absence of variation in the gastrointestinal tract size. Considering the spatial variability and seasonality of the region, a behavioural response is probably the most convenient strategy to overcome short-term environmental heterogeneity. In a plastic species such as P. xanthopygus, behaviour is a fundamental aspect to take into account by predictive models in the forecasts of climate change effects on biological diversity","palabras_clave":"BEHAVIOUR|DIET|PHENOTYPIC FLEXIBILITY|ELEVATION GRADIENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5941,"idioma_id":4,"titulo":"Combined high hydrostatic pressure and thermal treatments fully","resumen":"Theobjective of this work was to assess the possibility of obtaining acalcium-enriched soymilk with acceptable characteristics regarding toinactivation of trypsin inhibitors and lipoxygenase, protein solubility andphysical stability, through a combined thermal-high hydrostatic pressuretreatment. A Doehlert design was applied to study the effect of combinedpressure level (500 ? 700 MPa), initial temperature (45 ? 65 °C)and CaCl2 concentration (5 ? 15 mmol L-1). Results showedthat protein solubility was function of CaCl2 concentration (p &lt; 0.005),and that inactivation of trypsin inhibitors was function of pressure level andtemperature (p &lt; 0.005), lipoxygenase activity was totally inactivated inthe most assayed conditions. Physical stability was improved, depending oncalcium concentration no settling was detected or a less conspicuous phaseseparation was observed after the combined treatments. Our results indicatethat a combination of thermal and high hydrostatic pressure allowed theobtention of calcium-enriched soymilks with improved physical stability withoutadditives such as chelating agents, and acceptable in terms of inactivation oftrypsin inhibitors and lipoxygenase.","palabras_clave":"High hydrostatic pressure|soymilk|calcium|Trypsin inhibitors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5942,"idioma_id":4,"titulo":"Impact of introduced herbivores on understory vegetation along a regional moisture gradient in Patagonian beech forests","resumen":"Introduced ungulates can alter understory structure and composition posing a serious threat to forest biodiversity. Yet how large-herbivore impacts in forested regions vary along major environmental gradients remains little explored. If ungulate effects shift with habitat conditions, then management could be tailored to protect most vulnerable forests. We tested the hypothesis that the extent of livestock impact on understory vegetation increases with habitat moisture across Nothofagus dombeyi forests in Nahuel Huapi National Park, NW Patagonia, Argentina. Understory composition and species diversity were compared for paired sites (N = 5), which were historically used by cattle or remained free of livestock for more than 50 yr, and were located along a regional precipitation gradient (1500?2800 mm\/yr). Long-term cattle presence reduced the cover of sub-canopy trees, shrubs and bamboo by 57?83%, and increased the relative cover of ground-layer herbs, regardless of habitat moisture. Livestock effects on species composition increased towards the wettest forests, which contained more species exclusive to either browsed or unbrowsed sites. Livestock presence increased species richness (a diversity) and within-site heterogeneity (b diversity) in some locations, but mostly reduced species evenness (30%) throughout the moisture gradient. Species turnover at the gradient scale was lower across browsed sites than for livestock-free sites. Our results indicate that the historical presence of domestic cattle induced region-wide changes in understory communities, highlighting the vulnerable nature of the local flora to ungulate disturbance. The greater impact of livestock browsing on the species composition of wetter forests was consistent with the role of plant growth?defence trade-offs along resource gradients. We suggest that the erosion of understory vegetation attributed to domestic herbivores in Patagonian beech forests can be mitigated by adjusting current animal stocks, while moister forests should be given the highest conservation priority.","palabras_clave":"DISTURBANCE|DOMESTIC UNGULATES|HERBIVORES|FUNCTIONAL TYPES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5943,"idioma_id":4,"titulo":"Climate modifies response of non-native and native species richness to nutrient enrichment","resumen":"Ecosystem eutrophication often increases domination by non-natives andcauses displacement of native taxa. However, variation in environmental conditionsmay affect the outcome of interactions between native and non-nativetaxa in environments where nutrient supply is elevated. We examined theinteractive effects of eutrophication, climate variability and climate averageconditions on the success of native and non-native plant species using experimentalnutrient manipulations replicated at 32 grassland sites on fourcontinents. We hypothesized that effects of nutrient addition would be greatestwhere climatewas stable and benign, owing to reduced niche partitioning.We found that the abundance of non-native species increased with nutrientaddition independent of climate; however, nutrient addition increased nonnativespecies richness and decreased native species richness, with these effects dampened in warmer or wetter sites. Eutrophication also altered the timescale in which grassland invasion responded to climate, decreasing the importance oflong-term climate and increasing that of annual climate.Thus, climatic conditions mediate the responses of native and non-native flora to nutrient enrichment. Our results suggest that the negative effect of nutrient addition on native abundance is decoupled from its effect on richness and reduces the timescale of the links between climate and compositional change.","palabras_clave":"Nutrients|Climate variability|Network|Biological Invasions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5944,"idioma_id":4,"titulo":"ACTION OF FOSETYL-AL AND METALAXYL AGAINST PHYTOPHTHORA AUSTROCEDRI","resumen":"Fosetyl-Al and Metalaxyl, the most commonly used systemic fungicides against Phytophthora, were evaluated for their efficacy to control Phytophthora austrocedri, the pathogen that causes a serious disease at the Austrocedrus chilensis forests in Patagonia. The effect of the chemicals on pathogen development in vitro and in planta was analyzed. Both chemicals were shown to protect plants from the pathogen. In vitro assays showed that asexual reproduction was sensitive to both chemicals. However, mycelial growth and sexual reproduction, that were clearly sensitive to Metalaxyl, were sensitive only to high concentrations of Fosetyl-Al. Fosetyl-Al and Metalaxyl had almost the same efficacy when applied preventively by soil drench to seedlings. This difference between in vitro and in planta results can be attributed to the dual action of Fosetyl-Al, not only inhibiting the pathogen but also stimulating host defense. In adult trees preventive and curative treatments were tested but only the Fosetyl-Al preventive treatment was effective in the assayed conditions. Interestingly, seedlings pre-treated with both fungicides were less susceptible to the effectors secreted by the pathogen. Our results indicate that Fosetyl-Al and Metalaxyl provide some resistance to the plant besides the fungistatic direct action on the pathogen. Further studies to elucidate a possible resistance-inducing activity of these chemicals and the mechanisms involved are underway.  ACEPTADO JUNIO 2015","palabras_clave":"AUSTROCEDRUS CHILENSIS|MAL DEL CIPRES|AUSTROCEDRUS ROOT ROT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5945,"idioma_id":4,"titulo":"Complexity of leaf miner-parasitoid food webs declines with canopy height in Patagonian beech forests","resumen":"Abstract. 1. Consumer?resource species interactions form complex, dynamic net- works, which may exhibit structural heterogeneity at various scales. This study set out to address whether host?parasitoid food web size and topology vary across forest canopy strata, and to what extent foliar resources and species abundances account for vertical patterns in network structure.2. The vertical strati cation of leaf miner?parasitoid food webs was examined in two monotypic beech (Nothofagus pumilio) forests in northern Patagonia, Argentina. Quantitative food webs were constructed for separate canopy layers by sampling foliage from three tree-height classes at 0.5 ? 1, 2 ? 3 and 5 ? 6 m above ground.3. Leaf miner abundance per unit leaf mass and foliar damage (%) did not differ across strata, although foliage quality and quantity increased from the understorey to the upper canopy. Parasitism rates and food web complexity decreased with canopy height, as re ected by reduced linkage richness, linkage density, mean interaction strength, and host vulnerability.4. Null model analyses revealed that food web metrics, especially in the upper canopy, were often lower than expected when compared with randomly structured networks. Overall, these patterns held for two forests differing in vertical structure and in dominant miner morphotype and parasitoid species.5. These results suggest that vertical declines in network complexity may be driven by the parasitoids? limited functional response to host abundance and dispersal from pupation sites in the forest  oor. A broader constraint on food web structure seemed to be imposed by host?parasitoid trait matching, a re ection of large-scale assembly processes.","palabras_clave":"HERBIVORY|NETWORK STRUCTURE|NULL MODEL|PARASITISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5946,"idioma_id":4,"titulo":"Growth of (100)-highly textured BaBiO 3 thin films on silicon","resumen":"We report on the growth and characterization of non-epitaxial but (100)-highly textured BaBiO3 thin films on silicon substrates. We have found the deposition conditions that optimize the texture, and show that the textured growth is favoured by the formation of a BaO layer at the first growth stages. X-ray diffraction &#934;-scans, together with the observation that the same textured growth is found on films grown on Pt and SiO2 buffered Si, demonstrate the absence of epitaxy. Finally, we have shown that our (100)-oriented BaBiO3 films can be used as suitable buffers for the growth of textured heterostructures on silicon, which could facilitate the integration of potential devices with standard electronics.","palabras_clave":"BaBiO3|thin films|textured growth"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5947,"idioma_id":4,"titulo":"Chemical composition and physical properties of sorghum flour prepared from different sorghum hybrids grown in Argentina","resumen":"This work analyzed the physical, chemical, and thermal properties of sorghum flour and therelationships among these, in order to evaluate its suitability for the development of food products.Sorghum flour was obtained through roller dry milling from 20 commercial hybrids grown inArgentina with the average chemical composition of the samples being: 0.68% ash, 3.67% fat,12.21% protein, 83.45% total carbohydrates, 79.77% starch (amylose 26.6%), and 34.9mg of tannicacid per 100 g of flour. A high degree of variability among evaluated properties was found,particularly in the pasting properties peak viscosity (2809?5184mPa\/s), breakdown (1169?3170mPa\/s), and final viscosity (3030?4401mPa\/s) with onset temperature (To) and gelatinizationenthalpy (DH) varying between 66.8 and 72.6°C, and 5.38 and 8.48 J\/g, respectively. A principalcomponent analysis demonstrated that the grain color did not influence the chemical compositionof the flour. Cluster analysis permitted the separation of flour into three different groups withdifferent thermal and physicochemical characteristics, and enabled the selection of hybrids. Thus,sorghum flour is a versatile ingredient and can be used in several food and non-food applications.","palabras_clave":"sorghum|pasting|starch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5948,"idioma_id":4,"titulo":"Physicochemical and rheological characterization of Andean tuber starches: Potato (Solanum tuberosum ssp. Andigenum), Oca (Oxalis tuberosa Molina) and Papalisa (Ullucus tuberosus Caldas)","resumen":"The physicochemical and rheological properties of starches from four landraces of Andean potato (Cuarentona, Rosadita, Imilla and Waycha), and Oca and Papalisa tubers were investigated and compared against conventional potato starch. Proximate composition studies showed that the protein content in the studied starches varied between 0.29 % and 1.18 % (w\/w), the ash content between 0.24 % and 1.14 % (w\/w), and the lipid content between 0.14 % and 0.34 % (w\/w). Scanning electron microscopy investigations showed differences in shape of the granules of Andean potatoes and Oca and Papalisa. The mean size D[4,3] of the granules ranged between 23.3 and 48.11 &#956;m. Papalisa was the starch with the lowest AM content (20.4 %, w\/w) and Rosadita had the greatest (28.03 %, w\/w). The gelatinization enthalpy ranged from 18.7 to 14.8 J\/g and the gelatinization temperature between 65.5 and 60.8 °C. Viscograms of starch pastes showed that Cuarentona was the sample with the greatest peak viscosity (3152 cP) and presented the greatest final viscosity (3222 cP). The Andean potato and tuber starches exhibited low breakdown values. The relationship between physicochemical and rheological properties was analyzed by PCA. Results indicated that Oca and Papalisastarches were different from the Andean potato starches. Cuarentona starch showed the greatest K, G?, peak viscosity, final viscosity, and Oca starch the lowest ones. The protein content affected the peak viscosity and setback of the samples. Results suggest that these starches have similar properties to those of conventional potato starch.","palabras_clave":"andean tuber|starch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5949,"idioma_id":4,"titulo":"Differing nest-defence behaviour in urban and rural populations of breeding Burrowing Owls","resumen":"The behaviour of birds in urban habitats often differs from that of birds in surrounding natural and rural habitats, with the attenuation of fear responses to humans a primary behavioural adaptation to urban life. In breeding birds, fear responses and nest-defence have been linked to reproductive success. We studied the nest-defence behaviour of male Burrowing Owls in rural and urban habitats by measuring flight-initiation distance (FID), time to return to the nest following disturbance and aggressiveness of nest-defence in response to a person walking towards them during three stages of their breeding period: during incubation and during two nestling stages. Male Burrowing Owls breeding in rural habitat had longer FID than Owls breeding in urban habitat, but FID did not decrease over the breeding season whereas it did decrease over the breeding season in urban habitat. Male Owls in rural habitat were less likely to return to their nests within 10min of disturbance than Owls in urban habitat. Lastly, aggressiveness of nest-defence of Owls breeding in rural and urban habitats was similar and increased throughout the breeding season in both habitats. Our results highlight the role of behaviour in explaining the ability of Burrowing Owls to live in a range of habitats, including successfully breeding in urban areas, and emphasise the importance of breeding stage on behavioural traits.","palabras_clave":"AGGRESSION|ATHENE CUNICULARIA|BREEDING STAGE|FID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5950,"idioma_id":4,"titulo":"Development of a Mouse Model of Shiga Toxin 2 (STX2) Intoxication for Testing Therapeutic Agents against Hemolytic Uremic Syndrome (HUS)","resumen":"BACKGROUND: Hemolytic Uremic Syndrome (HUS) caused by infections with Shiga toxin (Stx)-producing E. coli is a life-threatening complication characterized by acute renal failure, thrombocytopenia and hemolytic anemia. Stx is the main pathogenic factor. Therefore, the mouse model by intravenous administration of a single lethal dose of Stx is often used to explore its pathogenic mechanisms.OBJECTIVE:The aim of this work was to develop an alternative mouse model of Stx type 2 (Stx2) intoxication to evaluate new therapeutic strategies.METHODS AND RESULTS: One lethal dose of Stx2 was divided in four daily doses. We observed a dose-dependent toxicity characterized by neutrophilia, leukocytopenia and renal damage. Most importantly, we demonstrated that the polyclonal anti-Stx2 serum was able to protect mice from fatal evolution even when administered together the third dose of Stx2.CONCLUSION: This model would provide an advantage for evaluation of therapeutic strategies. Furthermore, the results presented herein suggest that appropriate treatment with anti-Stx2 agents following the appearance of initial clinical signs may block the ongoing outcome or may alleviate disease in patients who have just been diagnosed with HUS. However, the delay in the onset of therapy would be unsafe.","palabras_clave":"HUS|Stx2|Mouse model|antibody therapy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5951,"idioma_id":4,"titulo":"First DNA Barcode Reference Library for the identification of South American Freshwater Fish from the Lower Paraná River","resumen":"Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I for a valid identification of 79 freshwater fish species from the Lower Paraná River. Neighbour-joining analysis based on K2P genetic distances formed non-overlapping clusters for almost all species with a &#8805;99% bootstrap support each. Identification was successful for 97.8% of species as the minimum genetic distance to the nearest neighbour exceeded the maximum intraspecific distance in all these cases. A barcoding gap of 2.5% was apparent for the whole data set with the exception of four cases. Within-species distances ranged from 0.00% to 7.59%, while interspecific distances varied between 4.06% and 19.98%, without considering Odontesthes species with a minimum genetic distance of 0%. Sequence library validation was performed by applying BOLDs BIN analysis tool, Poisson Tree Processes model and Automatic Barcode Gap Discovery, along with a reliable taxonomic assignment by experts. Exhaustive revision of vouchers was performed when a conflicting assignment was detected after sequence analysis and BIN discordance evaluation. Thus, the sequence library presented here can be confidently used as a benchmark for identification of half of the fish species recorded for the Lower Paraná River.","palabras_clave":"COI|Conservation|Genetics|Neotropical fish"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5952,"idioma_id":5,"titulo":"DESARROLLO DE RECIPIENTES ACTIVOS Y BIODEGRADABLES PARA CULTIVOS AGRÍCOLAS","resumen":"En este trabajo, se propone el desarrollo de recipientes biodegradables para cultivos que puedan ser trasplantados directamente y actuar como fertilizantes. Se eligió gelatina bovina como material base, que se procesó en una mini mezcladora-inyectora junto con una solución concentrada de urea que actuó como plastificante. Se realizaron estudios reológicos y de tracción para evaluar la inyección de las mezclas y las prestaciones mecánicas relacionadas con la aplicación propuesta. Considerando que estos materiales biodegradables tienen baja resistencia al agua, se propuso incrementar la estabilidad de los recipientes mediante recubrimiento superficial. Asimismo, se estudió y analizó su influencia en el contenido de humedad, materia soluble e hinchamiento en agua destilada. Se observó que las muestras recubiertas fueron significativamente más estables en agua que las muestras control, lo que evidencia la buena calidad y homogeneidad del recubrimiento, que avala la factibilidad del sistema seleccionado y su potencial desarrollo de contenedores biodegradables.","palabras_clave":"GELATINA|FERTILIZANTES|UREA|INYECCIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5953,"idioma_id":4,"titulo":"Growth hormone receptors in the atherinid fish Odontesthes bonariensis: characterization and expression profile after fasting-refeeding, and growth hormone administration","resumen":"In order to improve the understanding of pejerrey Odontesthes bonariensis, growth hormone (Gh)-insulin-like growth factor-1(Igf1) axis, O. bonariensis growth hormone receptor type 1 (ghr1) and type 2 (ghr2) mRNA sequences were obtained. Both transcripts were ubiquitously expressed except in kidney, encephalon and anterior intestine. Alternative transcripts of both receptors were found in muscle. Interestingly, two different ghr2 transcripts with alternative polyadenylation (APA) sites located in the long 3&#8242; untranslated region (UTR-APA) were also found in liver. Hepatic ghr1, ghr2 and insulin-like growth factor type 1 (igf1) transcript levels were examined under two different metabolic conditions. In the first experimental condition, fish were fasted for 2 weeks and then re-fed for another 2 weeks. Despite igf1 mRNA relative expression did not show significant differences under the experimental period of time examined, both ghr transcripts decreased their expression levels after the fasting period and returned to their control levels after re-feeding. In the second treatment, recombinant O. bonariensis growth hormone (r-pjGh) was orally administered once a week. After 4 weeks of treatment, liver igf1, ghr1 and ghr2 mRNA relative expression increased (13, 4·5 and 2·1 fold, P < 0·05) compared to control values","palabras_clave":"Alternative mRNA |Compensatory growth|Ghr|Pejerrey"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5954,"idioma_id":4,"titulo":"Evaluation of the genetic diversity of microsatellite markers among four strains of Oreochromis niloticus","resumen":"Different strains of Nile tilapia can be found worldwide. To successfully use them in breeding programs, they must be genetically characterized. In this present study, four strains of Nile tilapia?UFLA, GIFT, Chitralada, and Red-Stirling?were genetically characterized using 10 noncoding microsatellite loci and two microsatellites located in the promoter and first intron of the growth hormone (GH) gene. The two microsatellites in the GH gene were identified at positions ?693 to ?679 in the promoter [motif (ATTCT) 8] and in intron-1 at position +140 to +168 [motif (CTGT)7]. Genetic diversity was measured as mean numbers of alleles and expected heterozygosity, which were 4 45 and 0.60 (GIFT), 3.5 and 0.71 (UFLA), 4.5 and 0.57 (Chitralada), and 2.5 and 0.42 (Red-Stirling), respectively. Genetic differentiation was estimated both separately and in combination for noncoding and GH microsatellites markers using Jost?s DEST index. The UFLA and GIFT strains were the least genetically divergent (DEST = 0.10), and Chitralada and Red-Stirling were the most (DEST = 0.58). The UFLA strain was genetically characterized for the first time and, because of its unique origin and genetic distinctness, may prove an important resource for genetic improvement of Nile tilapia. This current study shows that polymorphisms found in coding gene regions might be useful for assessing genetic differentiation between strains.","palabras_clave":"STR|Tilapia|Growth Hormone|Genetic variability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5956,"idioma_id":4,"titulo":"Optimization of culture conditions for growth associated Cr(VI) removal by Wickerhamomyces anomalus M10","resumen":"Fernández P.M., Cruz E.L., Viñarta S.C. & L.I.C. de Figueroa (2016). Optimization of culture conditions for growth associated Cr(VI) removal by Wickerhamomyces anomalus M10. Bulletin of Environmental Contamination and Toxicology, ISSN: 0007-4861, in press.","palabras_clave":"Wickerhamomyces anomalus M10|Cr(VI) removal|Culture conditions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5957,"idioma_id":5,"titulo":"Desempeño térmico de cubiertas verdes en ciudades de zonas áridas","resumen":"Las cubiertas verdes constituyenuna estrategia de enverdecimiento urbano que contribuyen a reducir los efectosdel calentamiento global, disminuyendo las temperaturas urbanas y los consumos de energía en espacios interiores derivados delacondicionamiento térmico para alcanzar condiciones de confort en verano. Sinembargo, su implementación en zonas áridas está supeditada al uso de especiesvegetales de bajo consumo de agua. Este trabajo evalúa el desempeño térmico de cubiertas verdes con dos especies dedistinto hábito de crecimiento y requerimiento hídrico -Aptenia cordifolia y Sedumspectabile-. A tal fin se desarrollaron mediciones de variablesmeteorológicas en módulos experimentales, mediante microadquisidores de datos durantela estación verano 2015. Los resultados obtenidos demuestran que las cubiertas verdespermiten disminuir la temperatura media interior 2ºC, la amplitud térmica 8ºC y la temperatura máxima 6ºC con diferencias poco significativasentre las especies evaluadas (0.5°C).Estos valores reflejan la eficiencia térmica de dichas cubiertas y la necesidadde ampliarel número de especies evaluadas para determinar rangos de beneficio en torno aespecies de baja demanda hídrica y alta disponibilidad local.","palabras_clave":"Techos Verdes|Comportamiento Térmico Interior|Zonas Áridas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5959,"idioma_id":4,"titulo":"Comparability of Antibody-Mediated Cell Killing Activity Between a Proposed Biosimilar RTXM83 and the Originator Rituximab","resumen":"BACKGROUND:Biosimilars are described as biological products that resemble the structure of original biologic therapeutic products, with no clinically meaningful differences in terms of safety and effectiveness from the original. A wide range of biosimilars are under development or are already licensed in many countries. Biosimilars are earning acceptance and becoming a reality for immunotherapy treatments mainly based on the alternatives for the commercial anti-CD20 monoclonal antibody rituximab. The most important mechanism of action reported for this antibody is the induction of antibody-dependent cell cytotoxicity (ADCC), which is associated with the polymorphisms present at the 158 position in the IgG receptor Fc&#947;RIIIa.OBJECTIVE:The aim of the study was to validate the functional comparability between the proposed rituximab biosimilar RTXM83 and the original product. To achieve this we assessed the binding capacity and ADCC induction of this biosimilar, taking into account the different Fc&#947;RIIIa-158 polymorphisms.METHODS:Binding capacity was evaluated by flow cytometry using CD20 positive cells and a wide range of antibody concentrations. The Fc&#947;RIIIa-158 polymorphisms were analyzed by polymerase chain reaction (PCR) followed by allele-specific restriction enzyme digestion. ADCC was measured by a colorimetric lactate dehydrogenase-release assay, using effector cells from donors with different Fc&#947;RIIIa-158 polymorphisms.RESULTS:Binding capacity assay showed no differences between both products. Regarding ADCC, a similar relative potency was obtained between both antibodies, showing a higher response for the Fc&#947;RIIIa-158 valine\/valine (V\/V) polymorphism compared to the phenylalanine\/phenylalanine (F\/F), for both rituximab and RTXM83.CONCLUSION:Our data strongly suggest the biocomparability between the proposed biosimilar and the originator rituximab, in antibody recognition and ADCC activity. Additionally, our results suggest that donors with the Fc&#947;RIIIa-158V\/V polymorphism induce a higher ADCC response, as has been reported.","palabras_clave":"Biosimilar|Rituximab|ADCC|CD20"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5960,"idioma_id":4,"titulo":"Comparative analysis of actigraphy performance in healthy young subjects","resumen":"Sleep-related health disorders are increasing worldwide; diagnosis and treatment of such sleep diseases are commonly invasive and sometimes unpractical or expensive. Actigraphy has been recently introduced as a tool for the study of sleep and circadian disorders; however, there are several devices that claim to be useful for research and have not been thoroughly tested. This comparative study provides activity, sleep and temperature information regarding several of the most commonly used actigraphers: Micro-Mini Motion Logger; Act Trust; Misfit Flash; Fitbit Flex & Thermochron. Twenty-two healthy young subjects were assessed with five different commercial actigraphs (Micro-Mini Motionlogger Watch, Condor Act Trust, MisFit Flash and Fitbit Flex) and a temperature recorder (Thermochron), and also completed a sleep diary for a week. There were not significant differences in the analysis of rest-activity pattern between devices. Temperature rhythm comparison between the Act Trust and the Thermochron showed significant differences in rhythm percentage (p<0.05) and mesor (p<0.0563) but not in amplitude or acrophase. Although data accessibility and ease of use was very different for the diverse devices, there were no significant differences for sleep onset, total sleep time and sleep efficiency recordings, where applicable. In conclusion, depending on the type of study and analysis desired (as well as cost and compliance of use), we propose some relative advantages for the different actigraphy\/temperature recording devices.","palabras_clave":"ACTIGRAPHY|AMBULATORY MONITORING|CIRCADIAN|REST-ACTIVITY|SLEEP|TEMPERATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5961,"idioma_id":4,"titulo":"Smart lipid nanoparticles containing levofloxacin and DNase for lung delivery. Design and characterization","resumen":"Levofloxacin (LV) is a hydrophilic broad-spectrum antibiotic commonly used in pulmonary treatmentagainst recurrent infections of Pseudomonas aeruginosa, and particularly in cystic fibrosis (CF) disease. Inorder to study feasible carriers for LV, solid lipid nanoparticles (SLN) of myristyl myristate were preparedby the ultrasonication method in the presence of Pluronic®F68 under different experimental conditionsand characterized by dynamic light scattering, optical, transmission and scanning electron microscopyfor size and morphology. Alternatively, nanostructured lipid carriers (NLCs) were developed to improveLV encapsulation and storage. SLN showed 20.1 ± 1.4% LV encapsulation efficiency, while the NLCs encap-sulated 55.9 ± 1.6% LV. NLC formulation exhibited a more controlled release profile than SLN formulation,but both showed a biphasic drug release pattern with burst release at the first 5 h and prolonged releaseafterwards, demonstrated by in vitro tests. The hydrodynamic average diameter and zeta potential of NLCwere 182.6 ± 3.2 nm and &#8722;10.2 ± 0.2 mV, respectively, and were stable for at least 3 months. Additionally,DNase type I was incorporated into the formulations as a ?smart? component, since the enzyme couldhelp to decrease the viscoelasticity found in the lungs of CF patients and improves the antibiotic diffusion.FTIR, XRD, DSC, TGA and nitrogen adsorption isotherms of the nanoparticles indicate the presence of theloads in a noncrystalline state. The developed formulation showed an active antimicrobial activity againstP. aeruginosa and even against other opportunistic pathogens such as Staphylococcus aureus. The presenceof LV-loaded NLCs reduced the formation of a bacterial biofilm, which highlighted the significance of thenanodevice as a new alternative for CF treatment.","palabras_clave":"Levofloxacin|Solid Lipid Nanoparticles|Nanostructured lipid carriers|DNase|Cystic Fibrosis|Pulmonary delivery"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5962,"idioma_id":4,"titulo":"Nanodevices for the immobilization of therapeutic enzymes","resumen":"Therapeutic enzymes are one of the most promising applications of this century in the field of pharmaceutics. Biocatalyst properties can be improved by enzyme immobilization on nanoobjects, thereby increasing stability and reusability and also enhancing the targeting to specific tissues and cells. Therapeutic biocatalyst?nanodevice complexes will provide new tools for the diagnosis and treatment of old and newly emerging pathologies. Among the advantages of this approach are the wide span and diverse range of possible materials and biocatalysts that promise to make the matrix?enzyme combination a unique modality for therapeutic delivery.This review focuses on the most significant techniques and nanomaterials used for enzymeimmobilization such as metallic superparamagnetic, silica, and polymeric and single-enzymenanoparticles. Finally, a review of the application of these nanodevices to different  pathologies and modes of administration is presented. In short, since therapeutic enzymes constitute a highly promising alternative for treating a variety of pathologies more effectively, this review is aimed at providing the comprehensive summary needed to understand and improve this burgeoning area.","palabras_clave":"Biocatlizadores|Drug delivery|therapeutic enzymes|nanobiotechnology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5963,"idioma_id":4,"titulo":"NANOBIOTECHNOLOGY SOLUTIONS AGAINST Aedes aegypti","resumen":"Training in Tropical Diseases (TDR) program although was successfully applied for thelast 40 years by UNICEF\/UNDP\/World Bank\/WHO now changed due to currentchallenges and this active program requires new and innovative solutions. Amongneglected diseases, one of important issue in the world actually is infection throughAedes aegypti. Surveillance and control must be considered due to a great risk ofinfection with dengue, chikungunya and Zika viruses. Although several vaccinecandidates are in advanced stages of development no licensed dengue vaccine is yetavailable, then, new insides in term of development of pharmaceutical that scape fromresistance is of paramount importance. It is believed, in general, that the mostinnovative strategy at this time should be the use of nanobiotechnology in the search ofnew uses for old pharmaceuticals or new development of innovative and intelligentnanomedicines for neglected diseases. In this direction we focused this overview ondiagnostic assays as nanobiosensors, and nanomaterials against viruses, andnanoinsecticides on Aedes aegyptis control.","palabras_clave":"Aedes aegypti|mosquitoes|nanostructures|nanobiotechnology|antiviral|nanobiosensors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5964,"idioma_id":4,"titulo":"Slug is increased in vascular remodeling and induces a Smooth Muscle Cell proliferative phenotype","resumen":"Objective - Previous studies have confirmed Slug as a key player in regulating phenotypic changes in several cell models, however, its role in smooth muscle cells (SMC) has never been assessed. The purpose of this study was to evaluate the expression of Slug during the phenotypic switch of SMC in vitro and throughout the development of vascular remodeling. Methods and results - Slug expression was decreased during both cell-to-cell contact and TGF&#946; induced SMC differentiation. Tumor necrosis factor-&#945; (TNF&#945;), a known inductor of a proliferative\/dedifferentiated SMC phenotype, induces the expression of Slug in SMC. Slug knockdown blocked TNF&#945;-induced SMC phenotypic change and significantly reduced both SMC proliferation and migration, while its overexpression blocked the TGF&#946;-induced SMC differentiation and induced proliferation and migration. Genome-wide transcriptomic analysis showed that in SMC, Slug knockdown induced changes mainly in genes related to proliferation and migration, indicating that Slug controls these processes in SMC. Notably, Slug expression was significantly up-regulated in lungs of mice using a model of pulmonary hypertension-related vascular remodeling. Highly remodeled human pulmonary arteries also showed an increase of Slug expression compared to less remodeled arteries. Conclusions - Slug emerges as a key transcription factor driving SMC towards a proliferative phenotype. The increased Slug expression observed in vivo in highly remodeled arteries of mice and human suggests a role of Slug in the pathogenesis of pulmonary vascular diseases.","palabras_clave":"SMOOTH MUSCLE CELLS|PHENOTYPIC SWITCH|VASCULAR REMODELING|SLUG"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5965,"idioma_id":4,"titulo":"CFD model of a moderator tank for a pressure vessel PHWR nuclear power plant","resumen":"The CFD thermal hydraulic simulation of a Pressurized Heavy Water REactor (PHWR) moderator tank was performed. Heatingwas achieved by considering two heat sources, conduction\/convection through the coolant channels and neutron thermalization.For the first method, a suitable 2D model for coupling the 3D with a 1D code to solve the in-channel coolant was implemented. Forthermalization, a volumetric heat source was estimated. Due to the relevance of natural and forced convection in the heat transfer,the Boussinesq approach was assessed for high Rayleigh number flow before it was used to model the moderator tank. The resultsclarified the complex flow behaviour and the temperature field. It was noted that the thermal power transferred from the coolantwas not directly related to the fission power distribution but to the coolant temperature profile (nearly the same for all channels)and the non-homogeneous moderator temperature. The former profile was as expected for high-Rayleigh natural convection flows.","palabras_clave":"CFD|Heat Transfer|High Rayleigh number"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5966,"idioma_id":4,"titulo":"Numerical aspects of Eulerian gas-particles flow formulations","resumen":"An Eulerian two-fluid numerical solver with kinetic-frictional theory for granular flows has been implemented and validated on the open-source code platform OpenFOAM(R). Several aspects of the numerical treatment are discussed: maximum packing and phase disappearing limits, phase accumulation, cell-face fields interpolation and reconstruction practices, drag coupling approaches and different levels of conservativeness of the momentum equations. These last two topics are studied in depth. On the drag coupling analysis, it is observed that the partially implicit method (PIM) exhibits a convergence performance similar to the partial elimination algorithm (PEA) for a Geldart B particulate fluidized bed problem. But,for strongly coupled conditions (e.g. smaller particles) the use of the PEA becomes essential to meet a prescribed convergence criteria. Secondly, the conservativeness of the advective term of the momentum equations is analyzed by comparing three formulations of the advective term (the conservative form, the nonconservative form and the phase-intensive form). The impact of each formulation on the velocity field prediction is quantified for a shallow water problem and then extended to two-dimensional gas?liquid and gas?particle systems. The results show that the adoption of a conservative formulation is crucial to obtain accurate solutions in transient problems. However, for time-averaged analysis, which is often used for the study of fluidized bed systems, the nonconservative phase-intensive form is still a useful tool.","palabras_clave":"Conservative method|Two-fluid model|Granular flow|OpenFOAM|Fluidized bed"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5967,"idioma_id":4,"titulo":"Application of multi-factorial experimental design to successfully model and optimize inorganic chromium speciation by square wave voltammetry","resumen":"Response surface methodology (RSM) was used to optimize voltammetric signal of hanging mercury dropelectrode (HMDE) for inorganic chromium determination and speciation. Initial solution pH, DTPA(Diethylenetriaminepentaacetic acid) concentration, SWV frequency, pulse height, step potential, accumulationtime and accumulation potential were considered as important operating factors through experimental designmethodology for Cr(VI) quantification. The central composite and a Box?Behnken designs as response surface designcoupled with numerical optimization technique was applied for obtaining the optimum chemical and SWVinstrumental conditions as well as the maximum height of chromium reduction peak. Optimum conditions wereset as follow: pH 6.5, DTPA concentration 5.5 mmol L&#8722;1, SWV parameters: frequency 500 Hz, step potential0.005 V, pulse height 0.1 V, accumulation time 120 s and accumulation potential &#8722;1.10 V. Speciation is performedin two separate samples, in the first one Cr(VI) is measured after 40 min and DPTA addition. While in the secondone, Ce(IV) is added and all Cr(III) is oxidized to Cr(VI) allowing total chromium determination, finally Cr(III) isobtained by difference. Under the optimal experimental conditions, the current reduction peak was proportionalto chromium(VI) concentration in the 5?105 nmol L&#8722;1 range, with detection and quantification limits of 3.77 and8.47 nmol L&#8722;1, respectively. Using the proposed method, inorganic chromium speciation was successfully determinedin water samples with standard addition method, suggesting that this method can be applied to the quantificationof inorganic chromium speciation in water samples","palabras_clave":"Chromium speciation|Square wave voltammetry|Experimental design|Desirability profile"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5968,"idioma_id":4,"titulo":"Facing predation risk in aquatic systems: differential response of zooplankton and habituation to the false alarm","resumen":"In aquatic systems, alarm signals (physical and chemical) favor early detection of predators and promote the development of antipredation behaviors in preys. However, further studies are needed to understand how different the antipredation behavior can be among different species coexisting in a natural assembly. In this work, we conducted an indoor experiment with a zooplankton assemblage from a natural subtropical system to experimentally analyze their evasion behavior when exposed to an enclosed visual predator. We aimed to assess whether such behavior is different depending on the species within the assemblage and if they habituate to the alarm signals released by the predator after certain period without receiving any real attack. Ostracods, cladocerans and copepod nauplii evaded the predator but differed in the magnitude of the response as well as in the response time. The evasion behavior was not maintained in time and most organisms returned to an even distribution as time passed, suggesting that zooplankters may habituate to the alarm signals. Adult copepods and copepodites (here analyzed together) did not evade the fish but showed an almost homogeneous distribution over time. Rotifers moved over the time, but independently of the presence of fishes. Differential responses to the same alarm signals may contribute to determine the spatial distribution of zooplankton in natural aquatic ecosystems.","palabras_clave":"ANTIPREDATION BEHAVIOR|FISH PREDATION|FRESHWATER MICROCRUSTACEANS|ROTIFERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5969,"idioma_id":4,"titulo":"Evaluation of hyaluronic acid in cattle: physiological variations related to age, periparturition and in clinical cases of paratuberculosis","resumen":"Hyaluronic acid (HA) serum levels have been related to various pathological conditions in both humans and animals. The involvement of HA in the pathogenesis of mycobacterial infections has been suggested. The aim wasto evaluate serum levels and ilea tissue presence of HA in healthy bovines and in clinical paratuberculosis (PTB) cases. HA serum concentrations in bovines were found to be in the range of 130 to 617 ng\/mL with a median value of 418 ng\/mL. Significantly increased HA levels were detected in 1 month-old calves. Increased levels of HA were found at peripartum as compared to healthy control animals, with pre- parturition values significantly higher than post- parturition (p=0.02). Cows with clinical PTB showed higher serum levels and diminished ileal detection of HA than healthy animals. In serum, physiological variations in HA serum levels related to age and parturition were found in bovines. In addition, clinical PTB also affected the presence of HA in serum and ileum. These results might contribute to elucidate the clinical significance of HA evaluation in cattle and its involvement in PTB infection.","palabras_clave":"BOVINE|HYALURONIC ACID|AGE|PARATUBERCULOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5970,"idioma_id":4,"titulo":"Haematococcus pluvialis as a source of fatty acids and  phytosterols: potential nutritional and biological implications'","resumen":"main aim of this work was to describe the effect of light-induced stress on bioactive lipid accumulation in H. pluvialis CCALA 1081. To this end, the lipid profile was analyzed in order to determine triacylglyceride (TAG) and phytosterol content, the fatty acid profile of TAGs and the composition of the sterol fraction. After 3 days of light-induced stress, the content of both bioactive lipids significantly increased compared to controls. Palmitic, linoleic and &#945; linolenic fatty acid content was higher whereas caproic acid content diminished in H. pluvialis under stress. High light irradiance also reduced &#946;-sitosterol content whereas chlerosterol, brassicasterol and &#916;7-campesterol content was higher in the phytosterol fraction. No significant levels of lipid peroxidation were detected after 3 days of light-induced stress. In addition, H. pluvialis phytosterols displayed radical scavenging capacity and also a cytotoxic effect associated with the induction of apoptosis in human IMR-32 neuroblastoma cells. Our results show that high light stress induced the accumulation of commercially valuable compounds in H. pluvialis that are of potential benefit to human health.","palabras_clave":"Triacylglycerols|Phytosterols|Microalgae|Light-stress"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5971,"idioma_id":4,"titulo":"Challenges in Protein Formulation Focused on Extrusion-Spheronization Process","resumen":"iotechnology revolution had led the overcoming of different types of therapeutic protein because of theirchemical structure can perform specific reactions in the body, increasing efficacy and decreasing side effects.Numerous efforts were made to optimize the physicochemical properties of the proteins used for therapeuticand studied different methods for an effective administration of the protein contained in the medicine,evaluating different routes of administration to achieve the desired therapeutic effects. The delivery systemfor oral pharmaceutical proteins and peptides is still in development stage. There are number of limitationsto oral delivery of proteins such as barriers to peptide bioavailability after oral administration, intestinalmembrane permeability, size, intestinal and hepatic metabolism and solubility. Pellets have shown greatpotential in the delivery of proteins\/peptidal drugs. Some strategies of development of oral protein andpeptides has always been challenged, optimizing the safety and efficacy while ensuring the ability tomanufacture the drug while maintaining quality and stability. The pelletization techniques have beenreviewed in numerous papers, is a technique that enables the formation of spherical beads or pellets with amean diameter usually ranging from 0.5-2.0 mm. Pellets are prepared by different techniques, such asextrusion and spheronization. This review discusses challenges in protein formulation that have been used toprepare pelletized dosage forms using the extrusion-spheronization process.","palabras_clave":"DRUG DELIVERY|MULTIPLE UNIT SYSTEM|EXTRUSION-SPHERONIZATION|PELLETS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5973,"idioma_id":4,"titulo":"Surface expression of NMDA receptor changes during memory consolidation in the crab Neohelice granulata","resumen":"The aim of the present study was to analyze the surface expression of the NMDA-like receptors during the consolidation of contextual learning in the crab Neohelice granulata. Memory storage is based on alterations in the strength of synaptic connections between neurons. The glutamatergic synapses undergo various forms of N-Methyl-D aspartate receptor (NMDAR)-dependent changes in strength, a process that affects the abundance of other receptors at the synapse and underlies some forms of learning and memory. Here we propose a direct regulation of the NMDAR. Changes in NMDAR?s functionality might be induced by the modification of the subunit?s expression or cellular trafficking. This trafficking does not only include NMDAR?s movement between synaptic and extra-synaptic localizations but also the cycling between intracellular compartments and the plasma membrane, a process called surface expression. Consolidation of contextual learning affects the surface expression of the receptor without affecting its general expression. The surface expression of the GluN1 subunit of the NMDAR is down-regulated immediately after training, up-regulated 3 hours after training and returns to naïve and control levels 24 hours after training. The changes in NMDAR surface expression observed in the central brain are not seen in the thoracic ganglion. A similar increment in surface expression of GluN1 in the central brain is observed 3 hours after administration of the competitive GABAA receptor antagonist, bicuculline. These consolidation changes are part of a plasticity event that first, during the down-regulation, stabilises the trace and later, at 3 hours post training, changes the threshold for synapse activation.","palabras_clave":"Crabs, Neohelice granulata|NMDA receptor,|Context Signal Memory|Consolidation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5974,"idioma_id":4,"titulo":"High Resistivity Lipid Bilayers Assembled on Polyelectrolyte Multilayer Cushions: An Impedance Study","resumen":"Supported membranes on top of polymer cushions are interesting models of biomembranes as cell membranes are supported on a polymer network of proteins and sugars. In this work lipid vesicles formed by a mixture of 30% 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 70% 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) are assembled on top of a polyelectrolyte multilayer (PEM) cushion of poly(allylamine hydrochloride) (PAH) and poly(styrene sodium sulfonate) (PSS). The assembly results in the formation of a bilayer on top of the PEM as proven by means of the quartz crystal microbalance with dissipation technique (QCM-D) and by cryo-transmission electron microscopy (cryo-TEM). The electrical properties of the bilayer are studied by electrochemical impedance spectroscopy (EIS). The bilayer supported on the PEMs shows a high resistance, on the order of 107 &#937; cm2, which is indicative of a continuous, dense bilayer. Such resistance is comparable with the resistance of black lipid membranes. This is the first time that such values are obtained for lipid bilayers supported on PEMs. The assembly of polyelectrolytes on top of a lipid bilayer decreases the resistance of the bilayer up to 2 orders of magnitude. The assembly of the polyelectrolytes on the lipids induces defects or pores in the bilayer which in turn prompts a decrease in the measured resistance.","palabras_clave":"no posee"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5975,"idioma_id":5,"titulo":"Color, cenizas y capacidad antioxidante de mieles de la provincia de Buenos Aires, Argentina","resumen":"En el presente trabajo se caracterizaron 40 muestras de mieles de la provincia de Buenos Aires, Argentina, provenientes de siete regiones geográficas: Provincia Paranaense, Provincia del Espinal distrito del Talar y distrito del Caldén, Provincia Pampeana distrito Oriental, distrito Occidental y distrito Austral, y Provincia del Monte. El análisis palinológico demostró que la mitad de las mieles correspondían a mieles monoflorales. Se evaluó la capacidad antioxidante por medio de los ensayos de DPPH y FRAP, el contenido de fenoles totales (Folin), cenizas y color (Pfund) en función del origen floral y la procedencia geográfica. Se encontraron diferencias significativas en la actividad antioxidante, el contenido de cenizas, el color y el contenido de fenoles totales en muestras de miel de distintas regiones (p &#8804; 0,05). Sin embargo, mieles con distinto origen floral sólo presentaron diferencias significativas en el contenido de cenizas. Los resultados indican que la las mieles de Buenos Aires podrían ser caracterizadas mejor por su origen geográfico que por su origen floral.","palabras_clave":"MIEL|ACTIVIDAD ANTIOXIDANTE|ORIGEN GEOGRÁFICO|ORIGEN FLORAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5976,"idioma_id":5,"titulo":"Caracterización trófica de nueve especies de peces en los tramos Medio y Bajo del río Uruguay","resumen":"El objetivo del presente trabajo fue aportar información sobre la dieta de nueve especies de peces carnívoras del río Uruguay, a través del estudio de los contenidos digestivos. Se realizaron cinco campañas de muestreo ictiológico, entre septiembre de 2013 y marzo de 2015. Las localidades relevadas fueron Mocoretá (embalse de Salto Grande), Puerto Yeruá, Concepción del Uruguay y Gualeguaychú (?Aguas abajo? de la represa) y Villa Paranacito (en la zona de la desembocadura, con mayor influencia del Río de la Plata que las anteriores). La amplitud del nicho trófico mostró una mayor diversidad ?Aguas Abajo?, luego siguió Mocoretá y finalmente Villa Paranacito, lo que estaría indicando una mayor oferta de presas ?Aguas abajo?. Asimismo se observaron diferencias en la dieta de los peces analizados a lo largo del eje latitudinal del río en el tramo relevado. Las especies analizadas muestran un amplio espectro trófico, siendo los peces las presas dominantes, seguidos de invertebrados destacándose los moluscos invasores Corbicula fluminea y Limnoperna fortunei. En términos generales se puede concluir que las especies estudiadas son generalistas con un marcado predominio a la ictiofagia.","palabras_clave":"peces|trofica|rio Uruguay"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5977,"idioma_id":4,"titulo":"Ethanol downregulates N-acyl phosphatidylethanolamine-phospholipase D expression in BV2 microglial cells via epigenetic mechanisms","resumen":"Excessive ethanol drinking has deleterious effects on the brain. However, the effects of alcohol on microglia, the main mediator of the brain´s innate immune response remain poorly understood. On the other hand, the endocannabinoid system plays a fundamental role in regulating microglial reactivity and function. Here we studied the effects of acute ethanol exposure to murine BV2 microglial cells on N-acyl phosphatidylethanolamine-phospholipase D (NAPE-PLD), a major synthesizing enzyme of anandamide and other N-acylethanolamines. We found that ethanol downregulated microglial NAPE-PLD expression by activating cAMP\/PKA and ERK1\/2. These signaling pathways converged on increased phosphorylation of CREB. Moreover, ethanol induced an increase in histone acetyltransferase activity which led to higher levels of acetylation of histone H3. Taken together, our results suggest that ethanol actions on microglial NAPE-PLD expression might involve epigenetic mechanisms.","palabras_clave":"ETHANOL|MICROGLIA|EPIGENETIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5978,"idioma_id":4,"titulo":"Cordgrass canopy elicits weak effects on sediment properties and microphytobenthic abundance in a harsh environment","resumen":"The magnitude of the outcome of ecosystem engineering is context-dependent, as environmental stress increases, habitat amelioration by ecosystem engineers becomes more relevant. Moreover, individual- and population-level traits of ecosystem engineers seem to affect their ability to reduce environmental stress. In Argentinean Patagonian shores (SW Atlantic, 41° S), the intertidal community is exposed to harsh conditions. We predict that in this severe environment the variation of the above-ground structure of the smooth cordgrass Spartina alterniflora, should alter the buffering of the physical stress on the substrate, through shading and reduction of wind and water flow energy, affecting the sediment properties and microphytobenthic biomass. To test this hypothesis, we experimentally manipulated Spartina density in the edge and the inner marsh both in summer and winter. In summer, evaporation and temperature were higher in clipped plots, while in winter evaporation varied across densities and location treatments, with higher water content of the sediment inside the marsh. Neither effect of Spartina density nor location were observed on sediment organic matter and chlorophyll-a concentration, while mean grain size was always smaller inside the marsh. In summer, extracellular polymeric substances (EPS) content was higher in clipped plots inside the marsh, however this was not different from the other density treatments at this location. Overall, shading had weak effects on sediment properties and microphytobenthic biomass, and most observed differences can be attributed to grain size. We speculate that harsh conditions of this system overwhelm the ecosystem engineering effects of Spartina, probably because of its relatively small aboveground biomass.","palabras_clave":"SALTMARSH|ECOSYSTEM ENGINEERING|SPARTINA|ENVIRONMENTAL STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5979,"idioma_id":4,"titulo":"A role for the endocannabinoid system in the premature luteal regression and progesterone withdrawal in a LPS-induced early pregnancy loss model","resumen":"Study hypothesis: The aimof this study was to explore the role of the endocannabinoid system (eCS) in thealterations of the endocrine system in a murine model of lipopolysaccharide(LPS)-induced miscarriage.&nbsp;Study finding: We found that LPS increased the expression ofCOX-2 and the production of prostaglandin F2&#945; (PGF2&#945;) inthe uterus of 7-days pregnant mice, which resulted in a lower expression ofprolactin receptor in the ovary and a marked regression of corpora lutea (CL).These effects were absent in cannabinoid receptortype 1 knock-out (CB1-KO) mice, suggesting a role for the eCS as mediators of LPS-induceddeleterious effects on reproductive events. What is knownalready: Appropriate systemicprogesterone levels are critical for a successful pregnancy outcome. Precociousloss of luteal progesterone (P4) secretion leads to miscarriage in rodents. Wehave previously shown that LPS administration to pregnant mice inducesembryonic resorption accompanied by a dramatic decrease in systemicprogesterone levels in a murine model of inflammatory miscarriage, with theendocannabinoid system mediating these LPS-induced deleterious effects.Study design,samples\/materials, methods: CD1 wild-type (WT) and CB1-KO mice were used in this study. Animalswere randomly allocated to Vehicle- or LPS-treated groups originating fourgroups: WT-Vehicle; WT-LPS; CB1-KO-Vehicleand CB1-KO-LPS. Vehicle consisted onsaline solution injected i.p. LPS was administered i.p. in a single dose (0.5&#956;g\/g body weight) on day 7 of pregnancy and tissues (blood, ovary, uterus) werecollected at different time-points (6, 12, 24 and 48 hours).&nbsp; P4 and PGF2&#945; plasma levels were determined byradioimmunoassay. Cyclooxygenase-2 (COX-2) mRNA (RT-PCR) and protein (Westernblot) content in uterus was assayed. COX-2 and prolactin receptor (PrlR) mRNAlevels in the ovary were assayed by RT-PCR. Tissue morphology of the CL wasassessed by haematoxylin-eosin staining. Efforts were made to minimized thenumber of animals used in each study (n=5-8). Data were analyzed by one ortwo-way ANOVA in a completely randomized design. Post-hoc comparisons wereperformed with Tukey?s test. Normality and Homoscedasticity were tested withShapiro-Wilk (modified) test and Levene test, respectively. In cases were datadid not meet the assumptions, data transformation was applied and continued aspreviously described. Mean differences were considered significant whenp&lt;0.05.&nbsp; Main results andthe role of chance: Treatment with LPSto 7-day pregnant WT mice induced a P4 withdrawal (p&lt;0.05), increased inuterine COX-2 mRNA and protein expression (p&lt;0.05) as well as an increase inuterine PGF2&#945; production (p&lt;0.05). These changes were absent inLPS-treated 7-day pregnant CB1-KOmice. In ovarian tissues, LPS treatment to 7-day pregnant WT induced adownregulation of PrlR mRNA expression (p&lt;0.05) together with an increase inCOX-2 mRNA expression (p&lt;0.05) and PGF2&#945; content (p&lt;0.05).These effects were absent in the CB1-KOmice. Collectively, our results suggest a role for the eCS mediatingLPS-induced deleterious effects on reproductive tissues. ","palabras_clave":"Endocannabinoid system |Luteal regression |LPS|Early pregnancy loss"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5980,"idioma_id":4,"titulo":"Heparin exerts anti-apoptotic effects on uterine explants by targeting the endocannabinoid system","resumen":"Abstract Miscarriage caused by Gram-negative bacteriainfecting the female genital tract is one of the most commoncomplications of human pregnancy. Intraperitonealadministration of LPS to 7-days pregnant mice inducesembryo resorption after 24 h. Here, we show that LPSinduced apoptosis on uterine explants from 7-days pregnantmice and that CB1 receptor was involved in this effect. Onthe other hand, heparin has been widely used for the preventionof pregnancy loss in women with frequent miscarriagewith or without thrombophilia. Besides itsanticoagulant properties, heparin exerts anti-inflammatory,immunomodulatory and anti-apoptotic effects. Here, wesought to investigate whether the administration of heparinprevented LPS-induced apoptosis in uterine explants from7-days pregnant mice. We found that heparin enhanced cellsurvival in LPS-treated uterine explants and that this effectwas mediated by increasing uterine FAAH activity. Takentogether, our results point towards a novel mechanisminvolved in the protective effects of heparin.","palabras_clave":"MISCARRIAGE |CELL SURVIVAL|ENDOCANNABINOID SYSTEM|PREGNANCY LOSS|FAAH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5981,"idioma_id":4,"titulo":"Effects of pyrolysis conditions on the structure of chars prepared from an Argentine asphaltite","resumen":"As gasification reactivity of carbonaceoussolid material chars is known to be strongly dependent on their formationconditions, a comprehensive study was carried out on the effects of pyrolysisconditions on structure of chars prepared from an Argentine asphaltite. Thestudy comprised qualitative and quantitative analyses of char structures byX-ray diffraction, complemented with the characterization of physical morphologyof char particles by scanning electronic microscopy and high-resolutiontransmission electronic microscopy. Effects of pyrolysis temperature, heatingrate and holding time at the peak temperature were investigated separately in afixed bed tubular reactor. Additionally, the overall effect of variations intime-temperature history of individual char particles was evaluated using adrop tube furnace reactor. Experimental results have shown that pyrolysisconditions have a strong influence on the asphaltite-derived char structure,producing a significant reordering of the carbonaceous matrix at high heattreatment temperatures that is evidenced by increments in aromaticity andcrystalline carbon fraction. The resulting physical morphology ofasphaltite-derived char particles was found to be profoundly influenced by theheating rate at which the volatile matter is released, due to the appearance ofa liquid phase during the heating process.","palabras_clave":"Pyrolysis|Gasification|Asphaltite"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5982,"idioma_id":4,"titulo":". A new species of Gutierrezia (Asteraceae, Astereae, Solidagininae) from Argentinian Patagonia.","resumen":"A new species, Gutierrezia chubutensis (Asteraceae, Astereae, Solidagininae) from Florentino Ameghino in Chubut Province, Argentina is described and illustrated. It is distinct from its congeners in Patagonia because of its height (over 50 cm), larger elliptical leaves and numerous heads with yellow flowers and involucre terete or terete-turbinate. A key to differentiate the new species from the other Patagonian species of Gutierrezia is provided.","palabras_clave":"Gutierrezia|Asteraceae|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5983,"idioma_id":4,"titulo":"Architecture of genus Gutierrezia (Asteracea: Astereae, Solidagininae)","resumen":"An architectural study of the genus Gutierrezia Lag. was carried out, and seven different models were established. Differences in architecture between North American and South American species are pointed out. A key to differentiate the models is provided.","palabras_clave":"Asteraceae|Gutierrezia|Plant architecture|Astereae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5984,"idioma_id":4,"titulo":"Reinstatement of Gutierrezia leucantha Cabrera (Asteraceae, Astereae)","resumen":"Gutierrezia leucantha Cabrera, a species previously included in the synonymy of Gutierrezia gillesii Griseb., is reinstated as a distinct species. A morphological description is presented with information on distribution and habitat. A key to distinguish this species from its congeners with white ray florets is provided","palabras_clave":"Argentina|Asteraceae|Gutierrezia|Solidagineneae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5985,"idioma_id":4,"titulo":"The UVB1 Vitamin D Analogue Inhibits Colorectal Carcinoma Progression","resumen":"Vitamin D has been shown to display a wide variety of antitumour effects, but their therapeutic use is limited by its severe side effects. We have designed and synthesized a Gemini vitamin D analogue of calcitriol (UVB1) which has shown to display antineoplastic effects on different cancer cell lines without causing hypercalcemia. The aim of this work has been to investigate, by employing in silico, in vitro, and in vivo assays, whether UVB1 inhibits human colorectal carcinoma progression. We demonstrated that UVB1 induces apoptotic cell death and retards cellular migration and invasion of HCT116 colorectal carcinoma cells. Moreover, the analogue reduced the tumour volume in vivo, and modulated the expression of Bax, E-cadherin and nuclear &#946;-catenin in tumour animaltissues without producing toxic effects. In silico analysis showed that UVB1 exhibits greater affinity for the ligand binding domain of vitamin D receptor than calcitriol, and that several characteristics in the three-dimensional conformation of VDR may influence the biological effects. These results demonstrate that the Gemini vitamin D analogue affects the growth of the colorectal cancer and suggest that UVB1 is a potential chemotherapeuticagent for treatment of this disease.","palabras_clave":"CALCITRIOL|ANALOGUE|COLORRECTAL|CANCER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5986,"idioma_id":4,"titulo":"Atrial natriuretic peptide stimulates dopamine tubular transport by organic cation transporters: A novel mechanism to enhance renal sodium excretion","resumen":"The aim of this studywas to demonstrate the effects of atrial natriuretic peptide (ANP) on organiccation transporters (OCTs) expression and activity, and its consequences ondopamine urinary levels, Na+, K+-ATPase activity andrenal function. Male Sprague Dawley rats were infused with isotonic salinesolution during 120 minutes and randomized in nine different groups: control,pargyline plus tolcapone (P+T), ANP, dopamine (DA), D-22, DA+D-22, ANP+D-22, ANP+DAand ANP+DA+D-22. Renal functional parameters were determined and urinarydopamine concentration was quantified by HPLC. Expression of OCTs andD1-receptor in membrane preparations from renal cortex tissues were&nbsp; determined by western blot and Na+,K+-ATPase activity was determined using in vitro enzyme assay. 3H-DArenal uptake was determined in vitro. Compared to P+T group, ANP anddopamine infusion increased diuresis, urinary sodium and dopamine excretionsignificantly. These effects were more pronounced in ANP+DA group and reversedby OCTs blockade by D-22, demonstrating that OCTs are implied in ANPstimulated-DA uptake and transport in renal tissues. The activity of Na+,K+-ATPase exhibited a similar fashion when it was measured in thesame experimental groups. Although OCTs and D1-receptor protein expression werenot modified by ANP, OCTs-dependent-dopamine tubular uptake was increased byANP through activation of NPR-A receptor and protein kinase G as signalingpathway. This effect was reflected by an increase in urinary dopamineexcretion, natriuresis, diuresis and decreased Na+, K+-ATPaseactivity. OCTs represent a novel target that links the activity of ANP anddopamine together in a common mechanism to enhance their natriuretic anddiuretic effects.","palabras_clave":"ATRIAL NATRIURETIC PEPTIDE|DOPAMINE|RENAL|SODIUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5988,"idioma_id":4,"titulo":"Reconstruction of air shower muon densities using segmented counters with time resolution","resumen":"Despite the significant experimental effort made in the last decades, the origin of the ultra-high energy cosmic rays is still largely unknown. Key astrophysical information to identify where these energetic particles come from is provided by their chemical composition. It is well known that a very sensitive tracer of the primary particle type is the muon content of the showers generated by the interaction of the cosmic rays with air molecules. We introduce a likelihood function to reconstruct particle densities using segmented detectors with time resolution. As an example of this general method, we fit the muon distribution at ground level using an array of counters like AMIGA, one of the Pierre Auger Observatory detectors. For this particular case we compare the reconstruction performance against a previous method. With the new technique, more events can be reconstructed than before. In addition the statistical uncertainty of the measured number of muons is reduced, allowing for a better discrimination of the cosmic ray primary mass.","palabras_clave":"Ultra-high energy cosmic rays|Cosmic ray primary mass composition|Particle counters|Profile likelihood|Integrated likelihood"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5989,"idioma_id":4,"titulo":"Biodegradation of Bisphenol A and its  metabolic intermediates  by activated sludge: Stoichiometry and kinetics analysis","resumen":"Bisphenol A (BPA) has been described as a estrogenic compound. However, it is used in the production of polycarbonate, epoxy resins and other plastics. Mixed microbial cultures, such as activated sludge, have been proposed as a feasible methodology to achieve the decontamination of wastewater containing BPA. Several studies on the biodegradation of BPA have demonstrated the presence of 4-hydroxyacetophenone (4HAP), 4-hydroxybenzaldehyde (4HB) and 4-hydroxybenzoic acid (A4HB) asthe main metabolic intermediates. The objective of the present work was to study the kinetics and stoichiometric characteristics of the aerobic degradation of BPA and its metabolic intermediates by activated sludge. The effect of the sludge age (SA), and the concentration of each compound was analyzed. The biodegradation of the compounds was analyzed by an open respirometer. The kinetics of the biodegradation of all the tested compounds was influenced by the SA, the acclimation process, and the concentration of the tested compound. The stoichiometry of the reactions was not affected by the studied conditions. The oxidation coefficient (YO\/S) was 10.0 ± 0.5, 5.2 ± 0.2, 3.7 ± 0.2, and3.0 ± 0.1 molO2 molS1 for BPA, 4HAP, 4HB, and 4HBA, respectively. Based on the obtained YO\/S values, the aerobic biodegradation pathway of the tested compounds by BPA-acclimated activated sludge is discussed.","palabras_clave":"ACTIVATED SLUDGE|BISPHENOL A|KINETICS|STOICHIOMETRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5990,"idioma_id":4,"titulo":"Discovery of an activity cycle in the solar analog HD 45184. Exploring Balmer and metallic lines as activity proxy candidates","resumen":"Context. Most stellar activity cycles similar to that found in the Sun have been detected by using the chromospheric Ca II H&amp;K lines as stellar activity proxies. However, it is unclear if such activity cycles could be identified using other optical lines.Aims. To detect activity cycles in solar-analog stars and determine if these can be identified through other optical lines, such as Fe II and Balmer lines. We study the solar-analog star HD 45184 using HARPS spectra, whose temporal coverage and the high quality of the spectra allow us to detect both long and short-term activity variations.Methods. We analyse the activity signatures of HD 45184 by using 291 HARPS spectra obtained between 2003 and 2014. In order to search for line-core fluxes variations, we focus on Ca II H&amp;K and Balmer H&#945;, H&#946; lines, which are usually used as optical chromospheric activity indicators. We calculate the HARPS-S index from Ca II H&amp;K lines and convert it to the Mount-Wilson scale. In addition, we also consider as activity indicators the equivalent widths of Balmer lines. Moreover, we analyse the possible variability of Fe II and other metallic lines in the optical spectra. The spectral variations are analysed for periodicity using the Lomb-Scargle periodogram.Results. We report for the first time a long-term 5.14-yr activity cycle in the solar-analog star HD 45184 derived from Mount Wilson S index. This makes HD 45184 one of most similar stars to the Sun with known activity cycle. Such variation is also evident in the first lines of the Balmer series, which not always show a correlation with activity in solar-type stars. Notably, unlike the solar case, we also found that the equivalent widths of the high photospheric Fe II lines (4924 Å, 5018 Å and 5169 Å) are modulated (± 2 mÅ) by the chromospheric cycle of the star. These metallic lines show variations above 4&#963; in the RMS spectrum, while some Ba II and Ti II lines present variations at 3&#963; level which could be considered as marginal variations. From short-term modulation of the S index we calculate a rotational period of 19.98 days, which agrees with its mean chromospheric activity level. Then, we clearly show that theactivity cycles of HD 45184 could be detected in both Fe II and Balmer lines.","palabras_clave":"STARS: ACTIVITY|STARS: CHROMOSPHERES|STARS: SOLAR-TYPE|STARS: INDIVIDUAL: HD 45184"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5991,"idioma_id":4,"titulo":"Transference factors as a tool for the estimation of arsenic milk concentration","resumen":"The Chaco Pampean Plain of central Argentina representsone of the largest regions with high levels of arsenic(As) in groundwater. The aim of this study was the assessmentof a biotransference factor (BTF) as a tool for the estimation ofAs concentration in cow?s milk from As drinking water concentration.Total As content in livestock drinking water, soil,forage, and milk was determined in farms located in an area ofhigh As groundwater, in order to analyze the relation betweenAs uptake and its transfer to milk. The concentrations of As inmilk ranged from 0.5 to 8.0 &#956;g\/L. From the results obtained,drinking water may be considered the main source of exposureto As, and the biotransference factor for milk ranges from1.5× 10&#8722;5 to 4.3 ×10&#8722;4. Therefore, BTF provides a simple toolfor the estimation of arsenic levels in milk through the Aslivestock drinking water content.","palabras_clave":"ARSENIC|BIOTRANSFERENCE|MILK|DRINKING WATER|BIOTRANSFERENCE FACTOR|CATTLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5992,"idioma_id":4,"titulo":"The NSL chromatin-modifying complex subunit KANSL2 regulates cancer stem-like properties in glioblastoma that contribute to tumorigenesis","resumen":"KANSL2 is an integral subunit of the nonspecific lethal (NSL) chromatin-modifying complex that contributes to epigenetic programs in embryonic stem cells. In this study, we report a role for KANSL2 in regulation of stemness in glioblastoma (GBM), which is characterized by heterogeneous tumor stem-like cells associated with therapy resistance and disease relapse. KANSL2 expression is upregulated in cancer cells, mainly at perivascular regions of tumors. RNAi-mediated silencing of KANSL2 in GBM cells impairs their tumorigenic capacity in mouse xenograft models. In clinical specimens, we found that expression levels of KANSL2 correlate with stemness markers in GBM stem-like cell populations. Mechanistic investigations showed that KANSL2 regulates cell self-renewal, which correlates with effects on expression of the stemness transcription factor POU5F1. RNAi-mediated silencing of POU5F1 reduced KANSL2 levels, linking these two genes to stemness control in GBM cells. Together, our findings indicate that KANSL2 acts to regulate the stem cell population in GBM, defining it as a candidate GBM biomarker for clinical use.","palabras_clave":"KANSL2|Glioblastoma|GSCs|Neurosphere"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5993,"idioma_id":5,"titulo":"Determinación de la edad de pubertad en toritos Angus recriados en sistemas pastoriles de la Argentina","resumen":"Los objetivos de este trabajo fueron estimar la edad de inicio de la pubertad en toritos Angus recriados en sistemas pastoriles y comparar la eficacia de la determinación de características del eyaculado (concentración espermática y motilidad progresiva) y del perímetro escrotal como metodologías para definir la pubertad bajo condiciones de explotación comercial. Se evaluó el peso vivo, concentración espermática y motilidad progresiva, y el perímetro escrotal de 286 toritos Angus cada 28 días desde el destete a los 6 meses de edad hasta que alcanzaron la pubertad (perímetro escrotal: 27,9±0,2 cm; concentración espermática: 50x106\/mL y 10% de motilidad lineal). Se estratificó la población de toritos en cuatro cuartiles para el inicio de la pubertad y luego se los separó en dos poblaciones, los precoces (75% inferior) y los tardíos (25% superior). El análisis de sobrevivencia del momento del inicio de la pubertad para cada grupo se realizó utilizando el procedimiento LIFETEST del paquete estadístico SAS. La mediana de edad en que los toritos alcanzaron la pubertad determinada mediante una concentración espermática (>50x106\/mL, 10% de motilidad) fue similar a la obtenida usando el perímetro escrotal (28 cm; 287 vs. 291 días). El 50% de los toritos precoces fue púber 91 días antes que el mismo porcentaje de toros tardíos. El perímetro escrotal podría usarse como predictor de la pubertad en toritos recriados en condiciones extensivas. Discriminar el cuartil de animales sexualmente tardíos del resto de la población permitiría seleccionar a campo toros padres sexualmente precoces para rodeos comerciales. -16.1.1.69.","palabras_clave":"toros de un año|pubertad|perímetro escrotal|concentración espermática"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5994,"idioma_id":4,"titulo":"Cytokinin production by Pseudomonas fluorescens G20- 18 determines biocontrol activity against Pseudomonas syringae in Arabidopsis","resumen":"Plant beneficial microbes can mediate biocontrol of diseases either by directlyinterfering with pathogens or via strengthening the host. Although phytohormones, recently including cytokinins, were shown to regulate plantimmunity, their microbial production is not considered for biocontrol-effects. Here we identify Pseudomonas fluorescens G20-18 cytokinin-production as key determinant for biocontrol-effects against P. syringae in Arabidopsis, maintaining tissue integrity and ultimately biomassyield. While cytokinin-deficient loss-of-function mutants of G20-18 exhibit impaired biocontrol-effects, genetic complementation with cytokinin biosynthetic genes restores cytokinin-production and biocontrol-effects, whichcorrelates with differential cytokinin-levels in planta. Arabidopsis mutant analyses revealed the necessity of functional cytokinin-perception inplants combined with defense signaling by salicylic acid to fully establish thisbiocontrol-effect. This identification of microbial phytohormone-production as determinantof biocontrol-effects offers novel options for developing successful integratedplant protection strategies needed to cope with future challenges through increasing world population, climate change and restricted use of classical pesticides.","palabras_clave":"BIOCONTROL|CYTOKININ|ARABIDOPSIS|PSEUDOMONAS|INTERACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5995,"idioma_id":4,"titulo":"Evaluation of feline uterine and umbilical arteries blood flow in a pharmacologically induced abnormal gestation model","resumen":"The aim of this study was to describe resistance index (RI) and systolic\/diastolic ratio (S\/D ratio) of uterine and umbilical arteries in an experimental model of abnormal pregnancy in felids. On days 30 to 35 (32  2.9) after mating, 20 domestic short-hair pregnant queens were randomly assigned to one of the following treatment groups: a treated group (TG; n 8), which received 10 mg\/kg of aglepristone subcutaneously twice, 24 hours apart, and a control nontreated group (CG; n 12). M-mode and Doppler ultrasonographic evaluations were performed at the initiation of the treatment (Day 0) and then every other day during 8 days. In both groups, uterine and umbilical arteries were evaluated by Doppler ultrasound, whereas fetal heart rate was assessed by M-mode ultrasound. Resistance index of uterine artery augmented in TG from Day 2 onward, conversely it decreased in CG (P < 0.01). On Day 8, RI values were 0.64  0.05 vs 0.37  0.01 for TG and CG, respectively. Additionally, S\/D ratio of the same artery presented an increase in TG, whereas this ratio diminished in CG (P < 0.01). On Day 8, this parameter showed values of 2.98  0.4 vs 1.62  0.06 for TG and CG, respectively. Resistance index of umbilical artery remained almost unchanged in TG from Day 6 onward, whereas it progressively decreased in CG throughout the course of the study (P < 0.05). On Day 8, RI were 0.89  0.04 and 0.82  0.01, for TG and CG, respectively. Furthermore, on Day 8, S\/D ratio of umbilical artery progressively diminished in CG but not in TG (P < 0.01), being 14.7  9.1 vs 5.9  0.3 for TG and CG, respectively. Fetal heart rate was higher in TG than in CG (P < 0.05). Group differences in Doppler parameters appeared on Day 2, when the other clinical or ultrasonographic signs were still absent. It is concluded that blood flow of the uterine and umbilical arteries differed between these normal and abnormal gestations predicting an adverse obstetric outcome.","palabras_clave":"feline|Doppler|obstetric|pregnancy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5996,"idioma_id":4,"titulo":"Sublethal effects of acaricides and Nosema ceranae infection on immune related gene expression in honeybees","resumen":"Nosema ceranae is an obligate intracellular parasite and the etiologic agent of Nosemosis that affects honeybees. Beside the stress caused by this pathogen, honeybee colonies are exposed to pesticides under beekeeper intervention, such as acaricides to control Varroa mites. These compounds can accumulate at high concentrations in apicultural matrices. In this work effects of parasitosis\/acaricide on genes involved in honeybee immunity and survival were evaluated. Nurse bees were infected with N. ceranae and\/or were chronically treated with sublethal doses of coumaphos or tau-fluvalinate, the two most abundant pesticides recorded in productive hives. Our results demonstrate that: 1. honeybee survival was not affected by any of the treatments; 2. parasite development was not altered by acaricide treatments; 3. coumaphos exposure decreased lysozyme expression; 4. N. ceranae reduced levels of vitellogenin transcripts independently of the presence of acaricides. However, combined effects among stressors on imagoes were not recorded. Sublethal doses of acaricides and their interaction with other ubiquitous parasites in colonies, extending the experimental time, are of particular interest in further research work.","palabras_clave":"NOSEMA |ACARICIDAS|RESPUESTA INMUNE|APIS MELLIFERA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5997,"idioma_id":4,"titulo":"Excess Sorption of Supercritical CO2 within Cylindrical Silica Nanopores","resumen":"Using Molecular Dynamics simulations, we examine structural and dynamical properties of supercritical CO2 confined within cylindrical hydrophobic nanopores of diameters 38 and 10 Å. Computer simulations were performed along the isotherm T = 315 K, spanning CO2 densities from &#961;\/&#961;c = 2.22 down to &#961;\/&#961;c = 0.22. Radial and orientational distribution functions, analysis of interfacial dynamic properties, and estimatons for local diffusion and orientational relaxation times are presented. In agreement with previous experimental data, our simulation results reveal the presence of a dense phase adsorbed within the pores. The combination of low CO2 bulk densities and narrow pores leads to &#961;int\/&#961;blk &#8776; 5-fold enhancement of the global density of the confined fluid. These density increments gradually become much less marked as the external phase becomes denser. Contrasting, in that latter limit, we found that the trapped fluid may become less dense than the bulk phase. Adsorption behavior of CO2 onto hydrophilic-like and rugged pore surfaces were also exmined. In these cases, we observed a global slowdown in both translational and rotational motions for the trapped CO2, the largest retardations being those associated with spatial domains of the fluid located near the silica interface.","palabras_clave":"Supercritico|Absorcion CO2|Nanoconfinamiento|Dinamica Molecular"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":5999,"idioma_id":4,"titulo":"Effect of tannins on survival and development  of Daphnia menucoensis and the abundances of  bacteria","resumen":"Polyphenols ? products of organic-matter decomposition enteringwater bodies from autochthonous and especially allochthonoussources ? affect primary producers, bacterioplankton, andzooplankton to consequently modify food webs. Cladocerans arewidely used in research experiments because they constitute themost frequent prey of high-trophic-level organisms in the majorityof lakes, and certain species symbolise the ecologic prototype ofthe generalist filter feeder. In our study area?s shallow lakes,cladocerans, though generally of low abundance, do attainsignificantly high biomasses. We accordingly evaluated themortality of the cladoceran Daphnia menucoensis Paggi, 1996exposed to different tannin concentrations along with thebacterial abundance. In two experiments D. menucoensis femalesexposed to high tannin concentrations reproduced inefficiently,exhibited high mortality, and manifested altered behaviour, suchas reduced reflexes and diminished mobility. Though tanninsapparently affected algal abundances, the cladoceran herbivorystrongly reduced those numbers. Positive effects on bacteria,however, were not recorded. High-allochthonous-organic-mattercontainingwater bodies support trophic webs becausebacterioplankton provide an alternative energetic base for thezooplankton, as possibly occurs in shallow lakes of the SaladoRiverbasin, characterised by cyclic hydrologic periods alternatingbetween draught and flooding and intensive land use. Inconclusion, tannins diminish D. menucoensis?s survival, locomotion,and the capacity to respond to stimuli.","palabras_clave":"TANNINS|ZOOPLANKTON|DAPHNIA MENUCOENSIS|BACTERIOPLANKTON"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6000,"idioma_id":4,"titulo":"Chemical adsorption of phenacyl-1,2,3-benzotriazole over AMoO4 (0 1 0) scheelite surfaces. Structure and electronic properties","resumen":"Ab-initio DFT calculations are presented for the adsorption of phenacyl-1,2,3-benzotriazole on scheeliteAMoO4 (A = Ca, Sr and Ba) surfaces. Strong adsorption energy of the organic ligand on the scheelite surfaceis found. The formation of a bond with covalent character is due to the interaction between the highelectronic density of nitrogen and oxygen atoms of the organic molecule, with the empty valence orbitalsof the cationic species (A) of the scheelite. This interaction promotes a strongly stabilized interface and itmay explain the high reactivity toward nitrogen extrusion reactions in gas?solid experiments of scheelites.The energy band gap of the interface is found to decrease significantly upon molecular adsorption,indicating that this kind of adsorption could increase the conductivity of the interface.","palabras_clave":"Scheelite surface|Phenacyl-1,2,3-benzotriazole|Adsorption|Semiconductor interface"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6001,"idioma_id":4,"titulo":"Single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato","resumen":"Thegoal of this study was to estimate the single and joint effect of the basalregion of chromosome 2 and centromeric region of chromosome 8 on morphologicaland fruit quality traits in tomato (Solanumlycopersicum). The analysis was performed in a population derived from across between Rio Grande of S.lycopersicum and LA1589 of S.pimpinellifolium that segregates for both genomic regions. Four major QTLswere found on chromosome 2 and three on chromosome 8, all of them related withmorphological traits. QTLs for fruit shape index, proximal fruit end angle anddistal fruit end protrusion showed epistatic interaction. Both genomic regions (fs2.1 and fs8.1) explained 62%, 47% and 46% of the phenotypic variability forfruit shape index, proximal fruit end angle and distal fruit end protrusionrespectively. Minor QTLs were detected for other morphological and qualitytraits such as color, pH and fruit shelf life on chromosomes 2 and 8. Only singlegenomic region effects were found for quality trait. On the other hand, fs2.1 and fs8.1 regions control several fruit morphology attributes followinga digenic linear additive model with epistatic interactions. ","palabras_clave":"EPISTASIS|FRUIT COLOR|FRUIT SHAPE|fs2.1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6002,"idioma_id":4,"titulo":"Sucrose in bloom-forming cyanobacteria: loss and gain of genes involved in its biosynthesis","resumen":"Bloom-forming cyanobacteria are widely distributed in freshwater ecosystems. To cope with salinity fluctuations, cyanobacteria synthesize compatible solutes, such as sucrose, to maintain the intracellular osmotic balance. The screening of cyanobacterial genomes revealed that homologs to sucrose-metabolism related genes only occur in few bloom-forming strains, mostly belonging to Nostocales and Stigonematales orders. Remarkably, among Chroococcales and Oscillatoriales strains, homologs were only found in M. aeruginosa PCC 7806 and Leptolyngbya boryana PCC 6306, suggesting a massive loss of sucrose metabolism in bloom-forming strains of these orders. After a complete functional characterization of sucrose genes in M. aeruginosa PCC 7806, we showed that sucrose metabolism depends on the expression of a gene cluster that defines a transcriptional unit, unique among all sucrose-containing cyanobacteria. It was also demonstrated that the expression of the encoding genes of sucrose-related proteins is stimulated by salt. In view of its ancestral origin in cyanobacteria, the fact that most bloom-forming strains lack sucrose metabolism indicates that the genes involved might have been lost during evolution. However, in a particular strain, like M. aeruginosa PCC 7806, sucrose-synthesis genes were probably regained by horizontal gene transfer, which could be hypothesized as a response to salinity fluctuations.","palabras_clave":"sucrose metabolism|sucrose-phosphate synthase|sucrose-phosphate phosphatase|Microcystis aeruginosa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6003,"idioma_id":4,"titulo":"Physical seed dormancy in native legume species of Argentina","resumen":"Leguminosae is a family with high value of use for food, medicine, forage, ornamental and restoration ecology purposes. One obstacle for the use and management of many legume species is the presence of seeds with physical dormancy. Here, we evaluated the presence of physical dormancy in ninenative species of Argentina and identified possible methods for breaking dormancy. Caesalpinia gilliesii, Geoffroea decorticans, and Prosopis alpataco have seeds with no physical dormancy, whereas Crotalaria incana, C. pumila, C. stipularia, Desmanthus virgatus, Galactia texana, and Senna aphylla have seedswith physical dormancy. The most effective methods for breaking physical dormancy were mechanical and wet heat (100°C) scarification for Crotalaria spp.; mechanical, acid (20 and 30 min) and wet heat scarification (80 and 100°C) for D. virgatus; mechanical scarification for G. texana and mechanical and allacid scarification treatments for S. aphylla. These results contribute to the knowledge of the germination biology for these species, and are of particular interest for their propagation in glasshouse and for restoration and conservation programs.","palabras_clave":"Leguminosae|physical dormancy|scarification|seed germination"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6005,"idioma_id":4,"titulo":"Mycobiota associated\twith the ambrosia beetle Megaplatypus mutatus: Threat to poplar plantations","resumen":"Megaplatypus mutatus(syn.Platypus sulcatus) is an ambrosia beetle native to South America, which represents the main forest pest in Argentina of Poplar plantations and is also an emerging pest in Europe, representing a potentialrisk to forest and fruit plantations globally due to its low specificity. Knowledge of the interactions this insect has with microbes will be important in understanding its impacts and management. In this study, we characterized thefungal diversity associated with M. mutatus in Populus trees in Argentina. The fungal community of 28 attacked trees was studied by evaluating 1104 gallery fragments and 110 fragments of insects. Fungal isolates were identified using morphology and phylogenetic analysis of the internal transcribed spacer region of nDNA. Nineteen taxa were identified, the most relevantFusarium solanispecies complex, three species of Raffaelea and Graphium basitruncatum. Despite the lower frequency occurred by Raffaelea spp. and G. basitruncatum, we detected a specificity between male\/female and location in the gallery. Additionally, the topology of the galleries is described based on analyses with computed tomography and nuclear magnetic resonance. Enhancing these techniques, animage combining both data was produced, suggesting that the water circulation across outer sections might be involved in the modulation of the gallery topology","palabras_clave":"AMBROSIA BEETLE|FUSARIUM|RAFFAELEA|FUNGAL INTERACTION|COMPUTED TOMOGRAPHY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6006,"idioma_id":4,"titulo":"Development of natural waxes dispensers for pheromones and use in mating disruption of the ambrosia beetle Megaplatypus mutatus in poplar (Populus spp) plantations","resumen":"Megaplatypus mutatus (= Platypus mutatus) (Chapuis) is an ambrosia beetle native to South America that attacks live trees, mining deeply into the xylem through large tunnels. This activity weakens the structural integrity of the tree, causing severe stembreakage and mortality. Attacks are initiated by pioneer males that select a host tree and build shortnuptial galleries, to which they attract females using a sexual pheromone. Previously, we showed the potential for the strategy of pheromone-mediated mating disruption of M. mutatus in commercial poplar and hazelnut plantations in South America and Europe using polyethylene reservoir dispensers for pheromones. In the present work we replaced the polymeric reservoir dispensers by monolithic dispensers made by dispersion of the pheromone in natural waxes and the addition of kaolin and we found that: prior to pheromone deployment, the mean number of galleries per tree did not differ significantly between the control and treated plots and the same was observed after the mating disruption treatment for the control plot but not for treated plots, where the mean number of galleries were reduced. These findings confirm that mating disruption is a viable tool for management of M. mutatus in poplar plantations. Using natural wax dispensers has obvious advantages from an environmental point of view.","palabras_clave":"Ambrosia beetle|Pheromone|Wax dispenser|Controlled release"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6007,"idioma_id":4,"titulo":"Exploring nursery sites for oviparous chondrichthyans in the Southwest Atlantic (36°S-41°S)","resumen":"Theaimofthispaperwastoexploreegg-layingareasofoviparouschondrichthyansoccurringin the northern part of the Southwest Atlantic Ocean (36°S?41°S) between 50 and 200 metres depth and the Mar del Plata Canyon (from 200 to 3447 m). A total of 515 capsules were collected corresponding to 10 species. Four species accounted for 87.6% of the total catch: Psammobatis normani, Bathyraja macloviana, Amblyraja doellojuradoi and Bathyraja brachyurops. The remaining corresponded to Schroederichthys bivius, Psammobatis rudis, Zearaja chilensis, Bathyraja albomaculata, Psammobatis lentiginosa and Bathyraja sp. Most hauls were monospeci&#64257;c and relatively few hauls contained four or &#64257;ve species (southern part of the area, in sites located at 75 and 94?105 m depth). No egg capsules were recorded between 1712 m and 3447 m. Highest densities (>3000 capsules\/km2) were mainly found between 39°46.2&#8242; and 40°29.9&#8242;S from 85 to 105 m and they were also recorded near the Mar del Plata Canyon at 37°597&#8242;S and at 852 m. The highest value recorded was 12,326 capsules\/ km2 (located at 40°18.9&#8242;S and 85.4 m). The highest densities for each of the most abundant species are discussed. The &#64257;nding of high densities of egg cases near the Argentine shelfbreak front indicates that many skate species use this area as nursery sites. Egg-laying areas explored here are the &#64257;rst reported sites of this kind in the Southwest Atlantic Ocean. Recognizing and protecting egg-laying habitats may be an important step to a long-term conservation of oviparous chondrichthyan populations.","palabras_clave":"Argentine shelf-break front|Egg cases|Nursery sites|Oviparous chondrichthyans|Southwest Atlantic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6008,"idioma_id":5,"titulo":"La contribución argentina al conocimiento de los dinosaurios saurópodos: 1984-2016","resumen":"La finalidad del presente artículo es la indicar las contribuciones que los autores argentinos tuvieron sobre las ideas más relevantes con respecto al conocimiento de los Sauropoda en los últimos 30 años.","palabras_clave":"Sauropoda|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6009,"idioma_id":4,"titulo":"Symbiotic stars in X-rays III: Suzaku observations","resumen":"We describe the X-ray emission as observed by Suzaku from five symbiotic stars that we selected for deep Suzaku observations after their initial detection with ROSAT, ASCA, and Swift. We find that the X-ray spectra of all five sources can be adequately fit with absorbed optically thin thermal plasma models, with either single- or multi-temperature plasmas. These models are compatible with the X-ray emission originating in the boundary layer between an accretion disk and a white dwarf. The high plasma temperatures of kT > 3 keV for all five targets were greater than expected for colliding winds. Based on these high temperatures as well as previous measurements of UV variability and UV luminosity and the large amplitude of X-ray flickering in 4 Dra, we conclude that all five sources are accretion-powered through predominantly optically thick boundary layers. Our X-ray data allow us to observe a small optically thin portion of the emission from these boundary layers. Given the time between previous observations and these observations, we find that the intrinsic X-ray flux and the intervening absorbing column can vary by factors of three or more on a timescale of years. However, the location of the absorber and the relationship between changes in accretion rate and absorption are still elusive.","palabras_clave":"binaries: symbiotic, X-rays|individuals: CD -28 3719, EG And, Hen 3-461, Hen 3-1591"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6010,"idioma_id":4,"titulo":"SU Lyncis, a hard X-ray bright M giant: Clues point to a large hidden population of symbiotic stars","resumen":"Symbiotic star surveys have traditionally relied almost exclusively on low resolution optical spectroscopy. However, we can obtain a more reliable estimate of their total Galactic population by using all available signatures of the symbiotic phenomenon. Here we report the discovery of a hard X-ray source, 4PBC J0642.9+5528, in the Swift hard X-ray all-sky survey, and identify it with a poorly studied red giant, SU Lyn, using pointed Swift observations and ground-based optical spectroscopy. The X-ray spectrum, the optical to UV spectrum, and therapid UV variability of SU Lyn are all consistent with our interpretation that it is a symbiotic star containing an accreting white dwarf. The symbiotic nature of SU Lyn went unnoticed until now, because it does not exhibit emission lines strong enough to be obvious in low resolutionspectra. We argue that symbiotic stars without shell-burning have weak emission lines, and that the current lists of symbiotic stars are biased in favour of shell-burning systems. We conclude that the true population of symbiotic stars has been underestimated, potentially by a large factor.","palabras_clave":"binaries: symbiotic|stars: individual: SU Lyn|X-rays: binaries"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6011,"idioma_id":4,"titulo":"Physicochemical Properties and Field Evaluation of Monolithic Wax Formulations for the Controlled Release of a Forest Pest Pheromone","resumen":"This work shows the way to develop a monolithic device for the release of an active agent. Different waxes to prepare monolithic dispensers were used. Studies to characterize the releaserate of the pheromone dispensers, and to determine many physicochemical properties of the active components and waxes utilized as matrices, were conducted. The advantages of using monolithic devices as an insect pheromone dispenser in order to control an insect that attacks commercial poplar plantations were demonstrated. Also, the practical application employing monolithic dispensers manufactured in this paper with the sex pheromone of the ambrosia beetle Megaplatypus mutatus (Coleoptera: Platypodidae) was verified. This forest pest is known to attack tree plantations in South America. The release rates were mainly dependent of matrix hardness and viscosity, as well as the particle size of fillers. A good performance of the formulated monolithic dispensers was observed during the field trials, with a reduction of damage levels of 40%.","palabras_clave":"monolithic dispenser| controlled-release|Sulcatol|3-pentanol"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6012,"idioma_id":5,"titulo":"Hacia la conciencia cuántica a partir del efecto fotoeléctrico","resumen":"En este artículo se indaga acerca de los recursos que emplean los estudiantes cuando tienen que responder a interrogantes cuyo contenido se refiere a fenómenos intangibles desde el punto de vista sensorial que vinculan el mundo a distintas escalas, tal como es el caso de la interacción de la radiación electromagnética con la materia. El estudio se realizó sobre tres situaciones problemáticas de efecto fotoeléctrico, y a partir de su análisis se pudieron diferenciar claramente cinco categorías de respuestas que nos dirigen a reflexionar acerca de la construcción de un concepto esencial de las ciencias naturales, como es el de energía, y su relación con el de interacción radiación-materia, dualidad onda-partícula, cuanto, entre otros. Se evidencia una malla de subjetividades que actúa como soporte de la formación de conceptos hacia cuya objetividad apuntan los esfuerzos del proceso de enseñanza. Palabras clave interacción radiación-materia; energía; cuanto; mediciones macro y propiedades micro;","palabras_clave":"INTERACCION RADIACION-MATERIA|ENERGÍA|CUANTO|MEDICIONES MACRO Y PROPIEDADES MICRO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6013,"idioma_id":4,"titulo":"Reversible metallization of SnO2 films under hydrogen and oxygen containing atmospheres","resumen":"The adsorption of different gases onto tin oxidefilms surfaces promotes electrical conductance variations.Films chemiresistive properties depend on the intergranularbarrier heights and on the concentration of non-stoichiometricdefects that, in the case of tin oxide, are mainlyoxygen vacancies. We found that film exposures to an H2-containing atmosphere over 400 C led to unusual resultsthat cannot be explained resorting to regular interpretations.By means of Raman spectroscopy and XRD characterization,we show that, at high enough temperatures,when films are exposed to hydrogen, the tin dioxide convertsinto tin monoxide and metallic tin. Then, when filmsare exposed to air atmosphere, the oxygen diffuses into thegrains converting the metallic tin back into tin dioxide.These findings are consistent with electrical measurements.","palabras_clave":"TIN OXIDE|METALLIZATION|SENSOR FILM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6014,"idioma_id":4,"titulo":"Electrical behavior of BaSnO3 bulk samples formed by slip casting: effect of synthesis methods used for obtaining the ceramic powders","resumen":"In order to build bulk pieces using the slip casting technique, barium stannate (BaSnO3) ceramicpowders were synthesized using two chemical methods (controlled precipitation and Pechini).Differences in the electrical behavior of the pieces, depending on the synthesis method employed, werethen determined. For the analysis, a stable suspension of the synthesized ceramic powder (or 57.6 %solids slip) was obtained using water as solvent; the slip was poured into a mold to produceparallelepiped shaped green pieces. The electrical behavior in air at 300 °C was determined for piecessintered using a range of temperatures. The results of this electrical characterization showed that themethod of synthesis has strong effects on the electrical behavior of the sintered samples.","palabras_clave":"BaSnO3|ELECTRICAL BEHAVIOR|SLIP CASTING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6015,"idioma_id":4,"titulo":"Effective diffusion coef fi cient for Cu in steel joined by transient liquid phase bonding","resumen":"Seamless carbon steel tubes were joined by the transient liquid phase (TLP) bonding process using Cu foils as interlayers. Bonding was performed at 1300&nbsp;°C for seven minutes with an applied uniaxial pressure of 5&nbsp;MPa. The completion of isothermal solidification was not systematically achieved along the joint, leading to the presence of athermally solidified liquid (ASL). Consequently, the ability to predict the time to complete isothermal solidification - and therefore its kinetics - is of great interest. For this purpose, a one-dimensional model using the finite element method was employed to simulate the TLP bonding. In particular, regions where the completion of the process was not achieved provided a source for the effective diffusion coefficient (Def) for the bonding process. By knowing Def, different cases were considered within the proposed model, from the remaining time to complete the bonding process for an observed ASL, to the maximum liquid gap that can be isothermally solidified for a selected holding time. The validity of the utilization of Def was confirmed by analysing the diffusion kinetic regime of Cu in steel.","palabras_clave":"STEEL|TRANSIENT LIQUID PHASE BONDING|ISOTHERMAL SOLIDIFICATION|FINITE-ELEMENT SIMULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6016,"idioma_id":4,"titulo":"Recycling of Mixed Plastic Waste from Electrical and Electronic Equipment. Added value by compatibilization","resumen":"Plastic waste from electrical and electronic equipment (WEEE) grows up exponentially fast in the last two decades. Either consumption increase of technological products, like cellphones or computers, or the short lifetime of this products contributes to this rise generating an accumulation of specific plastic materials such ABS (Acrylonitrile-Butadiene-Styrene), HIPS (High impact Polystyrene), PC (Polycarbonate), among others. All of they can be recycled by themselves. However, to separate them by type is neither easy nor economically viable, then an alternative is recycling them together as a blend. Taking into account that could be a deterioration in final properties, to enhance phase adhesion and add value to a new plastic WEEE blend a compatibilization is needed. In this work, a systematical study of different compatibilizers for blends of HIPS and ABS from WEEE was performed. A screening analysis was carried out by adding two different compatibilizer concentration (2 wt% and 20 wt%) on a HIPS\/ABS physical blend 80\/20 proportion from plastic e-waste. Three copolymers were selected as possible compatibilizers by their possible affinity with initial plastic WEEE. A complete characterization of each WEEE was performed and compatibilization efficiency was evaluated by comparing either mechanical or morphological blends aspects. Considering blends analyzed in this work, the best performance was achieved by using 2% of styrene-acrylonitrile rubber, obtaining a compatibilized blend with double ultimate strength and modulus respect to the physical blend, and also improve mechanical properties of initial WEEE plastics. The proposed way is a promise route to improve benefit of e-scrap with sustainable, low costs and easy handling process. Consequently, social recycling interest will be encouraged by both ecological and economical points of view.","palabras_clave":"Plastic WEEE|Added value waste|Recycling|Blend compatibilization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6017,"idioma_id":4,"titulo":"Metals from mine waste as potential cause of oxidative stress in burrowing crab Neohelice granulata from San Antonio bay","resumen":"The NaturalProtectedAreaSanAntoniobayisofparticularimportanceforitscongregationofmigratoryshorebirdsandithasbeendeclaredoneoftheWesternHemisphereShorebirdReserveNetworkInter-national site(WHSRN).Presentstudyrepresentsthe first assessmentofvariationonoxidativestressbiomarkers inmalecrab Neohelice granulata from SanAntoniobay(RíoNegro,Argentina)under fieldconditions, associatedmainlytometalcontaminationcomingfrompassiveminingwastes.ThreesitesweresampledonceeverythreemonthsfromNovember2012toAugust2013withinthisseainlet(Pile,Fishery andPort)andacontrolsiteatthesoutheastofthebay(PuntaPerdices).AccumulationofNi,Zn,Cr andAlvariedonlywithseasonsalthoughwithoutaconstanttrend,meanwhileCd,CuandPbalsovariedamongsitesbeinghighestinPileandPort.Biochemicalresultsindicatedthatvariationsincatalaseactivity wasonlysitespecific beingmaximuminPile;meanwhilelipidradical, &#945;-tocopherol andme-tallothioneins wereonlyseasonalspecific beinghigherinautumnandwinter.Seasonalvariationwasalsofound fortotalthioles,beingthecontenthigherinsummerandautumnthaninwinter.Correlationanalysisrevealedthatmalondialdehydeand &#945;-tocopherolhaveapositiveassociationwithAlandne-gativewithNi,meanwhileGSThasapositiveassociationwithFe.Crabsfromtheclosestareatothewastepile didnotexhibitadifferentiatedoxidativepressuredespitethehigheraccumulationofmetals.Itispossible thatcrabsfromcontaminatedareashavedevelopedatolerancetometals,indicatingastrongecotoxicologicalselectivepressure.Morestudiesareneededtoassesswhetherthereisatransferofmetals throughthefoodchain.","palabras_clave":"TRACE METALS|MINING WASTES|BIOMARKERS|OXIDATIVE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6018,"idioma_id":4,"titulo":"Ovarian dysfunctions in adult female rat offspring born to mothers perinatally exposed to low doses of bisphenol A","resumen":"The study of oral exposure to the environmental estrogen bisphenol A (BPA) during the perinatal period and its effects on ovarian functionality in adulthood has generated special interest. Thus, our objective was to investigate ovarian folliculogenesis and steroidogenesis in adult female rat offspring born to mothers exposed to low doses of BPA (BPA50: 50 &#956;g\/kg day; BPA0.5: 0.5 &#956;g\/kg day) by the oral route during gestation and breastfeeding. Ovaries from both BPA-treated groups showed reduced primordial follicle recruitment and a greater number of corpora lutea, indicating an increased number of ovulated oocytes, coupled with higher levels of mRNA expression of 3&#946;-hydroxysteroid dehydrogenase and serum progesterone. BPA50-treated animals had lower expression of androgen receptor (AR) at different stages of the growing follicle population. BPA0.5-treated rats evidenced an imbalance of AR expression between primordial\/primary follicles, with higher mRNA-follicle-stimulating hormone receptor expression. These results add to the growing evidence that folliculogenesis and steroidogenesis are targets of BPA within the ovary. © 2015 Elsevier Ltd. All rights reserved.","palabras_clave":"Bisphenol A|Ovary|Folliculogenesis|Steroidogenesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6019,"idioma_id":4,"titulo":"Global gene expression and morphological alterations in the mammary gland after gestational exposure to bisphenol A, genistein and indole-3-carbinol in female Sprague-Dawley offspring","resumen":"This study aimed to evaluate the modifying effects of dietary genistein (GEN)and indole-3-carbinol (I3C) on early mammary gland development in female Sprague-Dawley offspring born to mothers exposed to BPA during gestation. Pregnant rats weretreated with BPA 25 or 250ug\/kg bw\/day from gestational day 10 to 21 with or withoutdietary intake of GEN (250 mg\/kg chow) or I3C (2000 mg\/kg chow). At post-natal day(PND) 21, female offspring from different litters were euthanized for mammary glanddevelopment and gene expression analyses. Our results indicated that prenatal exposureto BPA25 and 250 did not modify the ductal elongation of the mammary gland tree orthe estrogen receptor alpha (ER-a) expression in terminal end buds (TEBs). However,BPA25-exposed offspring had a higher number of terminal structures (TEBs+TDs) andan increased mammary branching and cell proliferation index in TEBs. Besides that,BPA25 and 250 modulated the expression of several genes in the immature mammarygland that were not changed in a dose dependent manner and involved different clustersof up- and down-regulated genes. Furthermore, BPA25 and BPA250+I3C-treatedgroups also had a higher number of enriched functional gene categories. In addition,maternal dietary GEN and I3C in association with BPA exposure produced specificgene expression alterations in the mammary gland and overcome the adverse effect ofBPA25, decreasing the branching of the mammary gland. In conclusion, prenatal BPAexposure induced both morphological and gene expression modifications on themammary gland that dietary intake of GEN and I3C reverted on BPA 25-exposedanimals.","palabras_clave":"BISPHENOL A|PREGNANCY|MAMMARY GLAND DEVELOPMENT AND GENE EXPRESSION|GENISTEIN|INDOLE-3-CARBINOL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6020,"idioma_id":4,"titulo":"ALLATOSTATIN-C ANTAGONIZES THE SYNERGISTIC MYOSTIMULATORY EFFECT OF ALLATOTROPIN AND SEROTONIN IN RHODNIUS PROLIXUS (Stal)","resumen":"Haematophagous insects can ingest large quantities of blood in a single meal producing a large quantityof urine in the following hours to eliminate the excess of water and mineral ions incorporated. The excretoryactivity of the Malpighian tubules is facilitated by an increase in haemolymph circulation as a resultof the intensification of aorta contractions, combined with an increase of anterior midgut peristalticwaves. We have recently shown that haemolymph circulation during post-prandial diuresis is modulatedby the synergistic activity of allatotropin (AT) and serotonin, resulting in an increase in aorta and cropcontraction rates. In the present study we describe the antagonistic effect of allatostatin-C (AST-C) onthe increase of aorta frequency of contractions induced by serotonin\/AT in Rhodnius prolixus. The administrationof AST-C counteracted the increase in the frequency induced by the treatment with serotonin\/AT, but did not affect the increase in frequency induced by the administration of serotonin alone, suggestingthat AST-C is altering the synergism between serotonin and AT. Furthermore, the administration ofAST-C during post-prandial diuresis decreases the number of peristaltic waves of the anterior midgut.The AST-C putative receptor is expressed in the hindgut, midgut and dorsal vessel, three critical organsinvolved in post-prandial diuresis. All together these findings provide evidence that AST-C plays a keyrole as a myoregulatory and cardioregulatory peptide in R. prolixus","palabras_clave":"Allatotropin|Allatostatin C|Rhodnius prolixus|Peptides"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6021,"idioma_id":4,"titulo":"SOMATOSTATIN SIGNALING SYSTEM AS AN ANCESTRAL MECHANISM: MYOREGULATORY ACTIVITY OF AN ALLATOSTATIN-C PEPTIDE IN HYDRA","resumen":"The coordination of physiological processes requires precise communication between cells.Cellular interactions allow cells to be functionally related, facilitating the maintaining ofhomeostasis. Neuropeptides functioning as intercellular signals are widely distributed inMetazoa. It is assumed that neuropeptides were the first intercellular transmitters, appearingearly during the evolution. In Cnidarians, neuropeptides are mainly involved inneurotransmission, acting directly or indirectly on epithelial muscle cells, and thereby controllingcoordinated movements. Allatostatins are a group of chemically unrelated neuropeptides thatwere originally characterized based on their ability to inhibit juvenil hormone synthesis ininsects. Allatostatin-C has pleiotropic functions, acting as myoregulator in several insects. Inthese studies, we analyzed the myoregulatory effect of Aedes aegypti Allatostatin-C in Hydra sp.,a member of the phylum Cnidaria. Allatostatin-C peptide conjugated with Qdots revealedspecifically distributed cell populations that respond to the peptide in different regions ofhydroids. In vivo physiological assays using Allatostatin-C showed that the peptide inducedchanges in shape and length in tentacles, peduncle and gastrovascular cavity. The observedchanges were dose and time dependent suggesting the physiological nature of the response.Furthermore, at highest doses, Allatostatin-C induced peristaltic movements of thegastrovascular cavity resembling those that occur during feeding. In silico search of putativeAllatostatin-C receptors in Cnidaria showed that genomes predict the existence of proteins of thesomatostatin\/Allatostatin-C receptors family. Altogether, these results suggest that Allatostatin-Chas myoregulatory activity in Hydra sp, playing a role in the control of coordinated movementsduring feeding, indicating that Allatostatin-C\/Somatostatin based signaling might be an ancestralmechanism.","palabras_clave":"Allatostatin C|Somatostatin|Neuropeptides|Hydra"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6022,"idioma_id":4,"titulo":"Characterization of the immune response induced by pertussis OMVs-based vaccine","resumen":"For the development of a third generation of pertussis vaccine that could improve the control of the disease, it was proposed that the immune responses induced by the classic whole cell vaccine (wP) or after infection should be used as a reference point. We have recently identified a vaccine candidate based on outer membrane vesicles (OMVs) derived from the disease etiologic agent that have been shown to be safe and protective in mice model of infection. Here we characterized OMVs-mediated immunity and the safety of our new candidate. We also deepen the knowledge of the induced humoral response contribution in pertussis protection. Regarding the safety of the OMVs based vaccine (TdapOMVsBp,) the in vitro whole blood human assay here performed, showed that the low toxicity of OMVs-based vaccine previously detected in mice could be extended to human samples.Stimulation of splenocytes from immunized mice evidenced the presence of IFN-&#947; and IL-17-producing cells, indicated that OMVs induces both Th1 and Th17 response. Interestingly TdapOMVsBp-raised antibodies such as those induced by wP and commercial acellular vaccines (aP) which contribute to induce protection against Bordetella pertussis infection. As occurs with wP-induced antibodies, the TdapOMVsBp-induced serum antibodies efficiently opsonized B. pertussis. All the data here obtained shows that OMVs based vaccine is able to induce Th1\/Th17 and Th2 mixed profile with robust humoral response involved in protection, positioning this candidate among the different possibilities to constitute the third generation of anti-pertussis vaccines.","palabras_clave":"Vaccines|Pertussis|OMVS|protection"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6023,"idioma_id":4,"titulo":"Vulnerability to climate change of Anolis allisoni in the mangrove habitats of Banco Chinchorro Islands, Mexico.","resumen":"As niche specialist species, lizards from tropical environments are characterized by a low tolerance and high physiological sensitivity to temperature changes. The extent of vulnerability to thermal changes depends on the lizard?s physiological plasticity to adjust the environmental changes. Herein we studied the thermal biology of Anolis allisoni, an endemic arboreal lizard from the tropical islands of the Banco Chinchorro Biosphere Reserve, Mexico, carried out during April and May 2012 and April 2014. We report field body (Tb) and preferred body temperatures in the laboratory (Tpref), operative temperatures (Te) and restriction of hours of activity. Anolis allisoni showed high and identical Tb and Tpref (33 °C), not significantly different than the mean Te (32.15 °C). The effectiveness of thermoregulation (E = -0.30) and the analysis of hours of restriction suggested that the high temperatures of Te (40 to 62.5 °C) registered at midday (from 12:00 to 15:00) of A. allisoni habitat are hostile and force lizards to take refuge during a period of 3 hrs of their daily time of activity. The scarcity of opportunities to find alternative refuges for thermoregulation in Banco Chinchorro point out the vulnerability of A. allisoni and the risk of local extinction when considering future predictions of increase in global environmental temperatures.","palabras_clave":"ANOLIS|TROPICAL ENVIRONMENT|GLOBAL WARMING|EXTINCTION|TEMPERATURE|VULNERABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6024,"idioma_id":4,"titulo":"The Sunflower Transcription Factor HaHB11 Improves Yield, Biomass and Tolerance to Flooding in Transgenic Arabidopsis Plants","resumen":"HaHB11 is a member of the sun&#64258;ower homeodomain-leucine zipper I subfamily of transcription factors. The analysis of a sun&#64258;ower microarray hybridized with RNA from HaHB11-transformed leaf-disks indicated&nbsp; the regulation of many genes encoding enzymes from glycolisis and fermentative pathways. A 1300&nbsp; bp promoter sequence, fused to the GUS reporter gene, was used to transform Arabidopsis plants showing&nbsp; an induction of expression after &#64258;ooding treatments, concurrently with HaHB11 regulation by submergence&nbsp;in sun&#64258;ower. Arabidopsis transgenic plants expressing HaHB11 under the control of theCaMV&nbsp; 35S promoter and its own promoter were obtained and these plants exhibited signi&#64257;cant increases in&nbsp; rosette and stem biomass. All the lines produced more seeds than controls and particularly, those of high&nbsp; expression level doubled seeds yield. Transgenic plants also showed tolerance to &#64258;ooding stress, both&nbsp; to submergence and waterlogging. Carbohydrates contents were higher in the transgenics compared to wild type and decreased less after submergence treatments. Finally, transcript levels of selected genes&nbsp; involved in glycolisis and fermentative pathways as well as the corresponding enzymatic activities were&nbsp;assessed both, in sun&#64258;ower and transgenic Arabidopsis plants, before and after submergence. Altogether, the present work leads us to propose HaHB11 as a biotechnological tool to improve crops yield, biomass and&#64258;oodingtolerance. ","palabras_clave":"TRANSCRIPTION FACTOR|FLOODING TOLERANCE|YIELD|BIOMASS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6025,"idioma_id":4,"titulo":"First U-Pb SHRIMP age for the Pilmatué Member (Agrio Formation) of the Neuquén Basin, Argentina: implications for the Hauterivian lower boundary","resumen":"Ammonite-based biostratigraphic schemes for the Lower Cretaceous are fairly well refined across the world, from the standard zonation in the West Mediterranean province to the Boreal and Austral provinces in the northern and southern hemispheres, respectively. However, the lack of radioisotopic ages associated to the fossil-rich, Lower Cretaceous marine successions has hindered the accurateestablishment of the numerical ages for the lower boundaries of its several stages (from Berriasian to Albian). Geochronological dating by UePb SHRIMP of a tuff layer that occurs within beds belonging to the Holcoptychites neuquensis Zone in the Pilmatue Member of the Agrio Formation in the Austral province (Neuquen Basin, Argentina) has resulted in an absolute age of 130.0  ± 0.6 Ma (2 sigma internal errors only) or 130.0 ± 0.8 Ma (including calibration and decay constant uncertainties). This age is interpreted to represent the time of eruption and thus the timing of the pyroclastic deposit. The H. neuquensis Zone is the equivalent of the A. radiatus Zone in the West Mediterranean province. Therefore, the obtained age is the first numerical data that could help constrain the Hauterivian lower boundary. Indeed, there is reasonable agreement with the latest proposed lower boundary of the Hauterivian at ~132.9 Ma. On the other hand, the duration recently established for this stage would be hard to reconcile with the stratigraphic record of the entire Hauterivian in the study region (northeastern Neuquen Basin). Therefore, the results of this contribution could also help to assess the extent of the Hauterivian and associated stages.","palabras_clave":"GEOCRONOLOGIA|CRETÁCICO|HAUTERIVIANO|CUENCA NEUQUINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6026,"idioma_id":4,"titulo":"Development of a dispersive liquid-liquid microextraction technique for the analysis of aryloxyphenoxy-propionate herbicides in soy-based foods","resumen":"In this work, an ionic liquid?dispersive liquid-liquid microextraction (IL-DLLME) method combined with liquidchromatography and diode-array detection (DAD) was used for the determination of four aryloxyphenoxypropionateherbicides (fenoxaprop-p-ethyl, quizalofop-p-tefuryl, propaquizafop and haloxyfop-p-methyl) intwo soy-based foods (soy milk and soy sauce) was used. For this purpose, the phosphonium-based roomtemperatureionic liquid (trihexyl(tetradecyl)phosphonium bistri&#64258;amide) was used as the extractant. The effect of theexperimental parameters on extraction ef&#64257;ciency such as type of disperser solvent, disperser solvent\/ ionic liquidvolumes ratio, pH, nature and concentration of salt in the aqueous phase, sample volume, and centrifugation andextraction times were investigated and optimized. Since matrix effects were detected, the standard additionmethod was used for quanti&#64257;cation. Under the optimized conditions, the proposed sample preparation methodcoupled to high performance liquid chromatography-diode array detection (HPLC-DAD) had a satisfactoryperformance to determine the four herbicides in soy sauce and soy milk. The enrichment factors ranged from18 to 43 and recovery factors from 25 to 66%. Although the recoveries were not high because of the presenceof organic solvent in the sample preparation step, the inter-day reproducibility was 8.4% or less, depending onthe analyte, the limits of detection (S\/N = 3) were obtained in the range of 0.12?0.34 mg L, the limits ofquanti&#64257;cation (S\/N = 10) between 0.36 and 1.04 mg L&#8722;1, and linear ranges from LOQs to 9.26 mg L.Finally,the IL-DLLME methodology is inexpensive, simple, fast, and environmentally friendly for the determination ofthe studied herbicides in soy sauce and soy milk. This study constitutes the &#64257;rst application of an IL-DLLMEmethodology to aryloxyphenoxy-propionate herbicides analysis in commercial soy-derived foods.&#8722;1&#8722;1","palabras_clave":"Aryloxyphenoxy-propionate herbicides|Phosphonium based room temperature ionic liquids|Ionic liquiddispersive liquid-liquid microextraction|soy-based foods"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6027,"idioma_id":4,"titulo":"Physiological response and sulphur accumulation in the biomonitor Ramalina celastri in relation to the concentrations of SO2 and NO2 in urban environments","resumen":"This study extends the current knowledge regarding the use of different tools for monitoring atmospheric pollution. The chemical response of the lichen Ramalina celastri was evaluated through physiological parameters and sulphur accumulation in relation to the SO2 and NO2 concentrations present in the air at the monitoring sites with different emission sources, in order to assess the atmospheric pollution in urban environments. In this way, it was possible to establish different levels of air quality using simultaneous measurements of gaseous pollutants in the air and of parameters for the exposed biomonitor, as well as to determine the relationship among them and their association with the different emission sources present. Thalli of Ramalina celastri were transplanted at sites with different pollutant emission sources within the city of Córdoba, and simultaneously the concentrations of SO2 and NO2 were measured at the same sampling sites. Pigment content, hydroperoxy conjugated dienes, malondialdehyde and sulphur accumulation were quantified in the lichen and the pollution index was calculated. The results showed that high concentrations of atmospheric SO2 in urban environments can be detected with certainty by measuring sulphur accumulation on the biomonitor. Although this relationship has often been assumed, it has not been confirmed by using simultaneous measurements. This biomonitor allowed different air quality levels to be identified in relation to the concentrations of NO2 and SO2 in Córdoba city. The biomarkers that best reflected the damage caused by pollutants on the lichen were pollution index, hydroperoxy conjugated dienes and malondialdehyde, which revealed a strong association with the most polluted areas in the city (industrial and heavy traffic sites).","palabras_clave":"MONITORING OF URBAN AIR POLLUTION|SO2|NO2|RAMALINA CELASTRI|PHYSIO-CHEMICAL DETERMINATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6028,"idioma_id":4,"titulo":"The complexity of nitric oxide generation and function in plants","resumen":"Plants are exposed to environmental stress, in natural and agricultural conditions.Nitric oxide (NO), a small gaseous molecule which plays important roles in plants, has been involved in many physiological processes, and emerged as an important endogenous signaling molecule in the adaptation of plants to biotic and abiotic stress. NO is produced from a variety of enzymatic and non enzymatic sources, which are not yet fully understood. Also, NO and reactive nitrogen species (RNS) can produce posttranslational modifications affectingprotein function. Nitrate reductase, a key enzyme in the nitrogen metabolism, is a proposed source of NO in plants which could be affected by posttranslational modifications.Thus, different pathways seem to be involved and can also regulate NO synthesis in the plant cell under physiological or stress conditions. However, how the levels of NO are reached in such time and place to fulfill its functions, are still puzzles to elucidate.","palabras_clave":"NITRATE REDUCTASE|POLYAMINES|POSTTRANSLATIONAL MODIFICATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6029,"idioma_id":4,"titulo":"Quantitative Risk Assessment of Human Trichinellosis Caused by Consumption of Pork Meat Sausages in Argentina","resumen":"In Argentina, there are three known species of genus Trichinella; however, Trichinella spiralis is most commonly associated with domestic pigs and it is recognized as the main cause of human trichinellosis by the consumption of products made with raw or insufficiently cooked pork meat. In some areas of Argentina, this disease is endemic and it is thus necessary to develop a more effective programme of prevention and control. Here, we developed a quantitative risk assessment of human trichinellosis following pork meat sausage consumption, which may be used to identify the stages with greater impact on the probability of acquiring the disease. The quantitative model was designed to describe the conditions in which the meat is produced, processed, transported, stored, sold and consumed in Argentina. The model predicted a risk of human trichinellosis of 4.88 x 10 -6 and an estimated annual number of trichinellosis cases of 109. The risk of human trichinellosis was sensitive to the number of Trichinella larvae that effectively survived the storage period (r = 0.89), the average probability of infection (PPinf) (r = 0.44) and the storage time (Storage) (r = 0.08). This model allowed assessing the impact of different factors influencing the risk of acquiring trichinellosis. The model may thus help to select possible strategies to reduce the risk in the chain of by-products of pork production.","palabras_clave":"Trichinelosis|Pork meat|Quantitative risk assesment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6030,"idioma_id":4,"titulo":"Profiles of alkali concentration and galactoglucomannan degradation in kraft impregnation of Scots pine wood: Experimental observations and modeling","resumen":"A deep understanding of alkali impregnation including the profiles of alkali concentration and hemicellulose degradation is necessary to analyze the optimization of current and innovative processes. In this paper, the impregnation of Scots pine (Pinus sylvestris L.) wood is analyzed and modeled under consideration of deacetylation, reactions of acidic group, and galactoglucomannan (GGM) degradation. The immobile anionic groups activated by the ionization of lignin-derived OH groups are also considered for the assessment of the Donnan effect. Predicted profiles of sodium and hydroxide ions and acetyl group concentrations are compared to experimental results. The balance between impregnation and hemicellulose degradation levels is discussed based on the developed models. Expectedly, higher concentrations and temperatures speed up the impregnation process. Nevertheless, a higher OH-concentration has two benefits for the same impregnation front position the alkali charge inside the chip is higher and the GGM degradation is lower.","palabras_clave":"acetyl group content|acid group reactions|deacetylation|Donnan effect|ionic diffusion|model validation|pulping process"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6031,"idioma_id":4,"titulo":"Emerging therapy in arthritis: Modulation of markers of the inflammatory process","resumen":"The induction of tolerance has been proposed as a therapeutic strategy for arthritis aiming to decrease progression of the pathology, probably by promoting suppressor mechanisms of the autoimmune response. This work aimed to confirm whether the treatment with vitamin D3 could synergize oral tolerance induced by hydrolyzed collagen peptides, in our experimental model of antigen induced arthritis in New Zealand rabbits. Clinical observation of the phenomenon indicates that simultaneous treatment with hydrolyzed collagen peptides and vitamin D3 was beneficial when compared with no treatment, for arthritic animals, and for arthritic animals that received treatment with only hydrolyzed collagen peptides or vitamin D3. Treatment with hydrolyzed collagen peptides caused diminished proinflammatory cytokine levels, an effect synergized significantly by the simultaneous treatment with vitamin D3. The anatomical-pathological studies of the animals that received both treatments simultaneously showed synovial tissues without lymphocytic and plasma cell infiltrates, and without vascular proliferation. Some of the synovial tissue of the animals of these groups showed a slight decrease in Galectin-3 expression. We propose that simultaneous oral treatment with vitamin D3 and hydrolyzed collagen peptides could increase the immunoregulatory effect on the process of previously triggered arthritis. We used articular cartilage hydrolysate and not collagen II because peptides best expose antigenic determinants that could induce oral tolerance. Oral tolerance may be considered in the design of novel alternative therapies for autoimmune disease and we have herein presented novel evidence that the simultaneous treatment with vitamin D3 may synergize this beneficial effect.","palabras_clave":"artritis|tolerancia oral|vitamina D|Inmunomodulación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6032,"idioma_id":4,"titulo":"Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone","resumen":"The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis hasbeen well-established in birds and mammals. However, its role in other vertebrates, such as the tel-eost fish, remains controversial. In this context, the present study aimed to evaluate whether GnIHmodulates the release of gonadotrophins and growth hormone (GH) in the cichlid fish Cichlasomadimerus. First, we partially sequenced the precursor polypeptide for GnIH and identified three puta-tive GnIH peptides. Next, we analysed the expression of this precursor polypeptide via a polymerasechain reaction in the reproductive axis of both sexes. We found a high expression of the polypep-tide in the hypothalamus and gonads of males. Immunocytochemistry allowed the observation ofGnIH-immunoreactive somata in the nucleus posterioris periventricularis and the nucleus olfacto-retinalis, with no differences between the sexes. GnIH-immunoreactive fibres were present in allbrain regions, with a high density in the nucleus lateralis tuberis and at both sides of the thirdventricle. Finally, we performed in vitro studies on intact pituitary cultures to evaluate the effect oftwo doses (106M and 108M) of synthetic C. dimerus (cd-) LPQRFa-1 and LPQRFa-2 onthe release of gonadotrophins and GH. We observed that cd-LPQRFa-1 decreased b-luteinising hor-mone (LH) and b-follicle-stimulating hormone (FSH) and also increased GH release to the culturemedium. The release of b-FSH was increased only when it was stimulated with the higher cd-LPQRFa-2 dose. The results of the present study indicate that cd-LPQRFa-1, the cichlid fish GnIH,inhibits LH and FSH release and stimulates GH release in intact pituitary cultures of C. dimerus. Theresults also show that cd-LPQRF-2 could act as an FSH-releasing factor in this fish species.","palabras_clave":"GONADOTROPHIN-INHIBITORY HORMONE|GROWTH HORMONE|FOLLICLE-STIMULATING HORMONE|LUTEINISING HORMONE|FISH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6033,"idioma_id":5,"titulo":"Así comienza la vida plaquetaria: un viaje desde los megacariocitos medulares a las plaquetas circulantes","resumen":"Perhaps for being considered as mere megakaryocyte cytoplasmic debris responsible for wound repair only, platelets have had a secondary role when compared to other cellular blood components. However, in the last 20 years we have learned much more about these fascinating cells, which have slowly regained a prominent place in hematology. In this review, we discuss the most outstanding and novel contributions on platelet biogenesis, its regulation by the bone marrow microenvironment and humoral factors, analyzing from megakaryocyte generation to platelet release.","palabras_clave":"PLATELETS|MEGAKARYOCYTES|HEMATOPOIESIS|HROMBOPOIESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6034,"idioma_id":4,"titulo":"Structural differences in cortical shell properties between upper and lower human fibula as described by pQCT serial scans. A biomechanical interpretation","resumen":"This study describes the structural features of fibula cortical shell as allowed by serial pQCT scans in 10\/10 healthy men and women aged 20?40 years. Indicators of cortical mass (mineral content -BMC-, cross-sectional area -CSA-), mineralization (volumetric BMD, vBMD), design (perimeters, thickness, moments of inertia -MIs-) and strength (Bone Strength Indices, BSIs; polar Strength-Strain Index, pSSI) were determined. All cross-sectional shapes and geometrical or strength indicators suggested a sequence of five different regions along the bone, which would be successively adapted to 1. transmit loads from the articular surface to the cortical shell (near the proximal tibia-fibular joint), 2. favor lateral bending (central part of upper half), 3. resist lateral bending (mid-diaphysis), 4. favor lateral bending again (central part of the lower half), and 5. resist bending\/torsion (distal end). Cortical BMC and the cortical\/total CSA ratio were higher at the midshaft than at both bone ends (p < 0.001). However, all MIs, BSIs and pSSI values and the endocortical perimeter\/cortical CSA ratio (indicator of the mechanostat´s ability to re-distribute the available cortical mass) showed a ?W-shaped? distribution along the bone, with maximums at the mid-shaft and at both bone´s ends (site effect, p < 0.001). The correlation coefficient (r) of the relationship between MIs (y) and cortical vBMD (x) at each bone site (?distribution\/quality? curve that describes the efficiency of distribution of the cortical tissue as a function of the local tissue stiffness) was higher at proximal than distal bone regions (p < 0.001). The results from the study suggest that human fibula is primarily adapted to resist bending and torsion rather than compression stresses, and that fibula´s bending strength is lower at the center of its proximal and distal halves and higher at the mid-shaft and at both bone´s ends. This would favor, proximally, the elastic absorption of energy by the attached muscles that rotate or evert the foot, and distally, the widening of the heel joint and the resistance to excessive lateral bending. Results also suggest that biomechanical control of structural stiffness differs between proximal and distal fibula.","palabras_clave":"FIBULA|BONE STRUCTURE|BONE BIOMECHANIC|BONE MECHANOSTAT|BONE ADAPTATION|BONE ARCHITECTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6035,"idioma_id":4,"titulo":"Synopsis of Hexasepalum (Rubiaceae), the Priority Name for Diodella and a New Species from Brazil","resumen":"Hexasepalum is the priority name for Diodella. In the present paper, we provide a synopsis of Hexasepalum. The genus differs from the remaining genera of the tribe Spermacoceae by the combination of infundibuliform flowers, 2-lobed stigmas,schizocarpic fruits, ventral face of the mericarps with two depressions, seeds with T or Y shaped ventral grooves and pollen grains with two types of supratectal elements. We propose two combinations, i.e. Hexasepalum domingense and H. vaginale, and the formal synonymization of H. angustifolium (including Diodella crassifolia). We also propose two new synonyms: Diodia rosmariinifolia [=Diodella rosmariinifolia] is placed in the synonymy of H. apiculatum and Diodia incana is now considered a synonym of H. teres.We also propose two lectotypifications for two synonyms of H. apiculatum and the lectotypification for H. vaginale. In addition, we describe and illustrate a new species endemic from Bahia, Brazil, H. zappiae sp. nov. Detailed descriptions are provided only for the species imperfectly known; otherwise comprehensive taxonomic descriptions are mentioned for each species. The descriptions of the Hexasepalum species are complemented with a key, geographical distributions and illustrations of key morphological characters. Line drawings are provided for species not illustrated before in literature.","palabras_clave":"Diodia|Neotropic|Spermacoceae|taxonomy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6036,"idioma_id":4,"titulo":"'Is it a question of consistency? a reply to Bybee et al. and Sahlén et al.","resumen":"This contribution seeks to broaden the discussion raised by Bybee et al.and Sahlén et al. on the correct use of the terms larva, nymph and naiadby including an evolutionary perspective","palabras_clave":"Larvae|Nymph|Naiad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6037,"idioma_id":4,"titulo":"Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor","resumen":"Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study  aimed to select ethanol-producing yeasts, after isolating samples from molasses  obtained from companies in the Province of Tucumán (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and A11 isolates showed higher ethanol productions of  12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and A11 was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory sclae was faster using a sugarcene molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products.  These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising  microorganism for the production of bioethanol with potential environmental, energy and economic benefits to  be projected into industrial scale.","palabras_clave":"BIOETHANOL|SCALE UP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6039,"idioma_id":4,"titulo":"Micro-CT scan reveals an unexpected high-volume and interconnected pore network in a Cretaceous Sanagasta dinosaur eggshell","resumen":"The Cretaceous Sanagasta neosauropod nesting site (La Rioja, Argentina) was the first confirmed instance of extinct dinosaurs using geothermal-generated heat to incubate their eggs. The nesting strategy and hydrothermal activities at this site led to the conclusion that the surprisingly 7 mm thick-shelled eggs were adapted to harsh hydrothermal microenvironments. We used micro-CT scans in this study to obtain the first three-dimensional microcharacterization of these eggshells. Micro-CT-based analyses provide a robust assessment of gas conductance in fossil dinosaur eggshells with complex pore canal systems, allowing calculation, for the first time, of the shell conductance through its thickness. This novel approach suggests that the shell conductance could have risen during incubation to seven times more than previously estimated as the eggshell erodes. In addition, micro-CT observations reveal that the constant widening and branching of pore canals form a complex funnel-like pore canal system. Furthermore, the high density of pore canals and the presence of a lateral canal network in the shell reduce the risks of pore obstruction during the extended incubation of these eggs in a relatively highly humid and muddy nesting environment.","palabras_clave":"titanosaurs|hydrothermal incubation|burrow-nesting|Sanagasta|micro-CT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6040,"idioma_id":4,"titulo":"Multiple approach for identification of clinically infrequent non tuberculous mycobacteria in Argentina","resumen":"SETTING: Over 150 potentially pathogenic non tuberculous mycobacteria (NTM) species have been described, posing an onerous challenge to clinical laboratory diagnosis. OBJECTIVE: To evaluate different approaches to attain identification for 40 clinically relevant NTM isolates whose species were not reliably resolved by our routine diagnostic workflow consisting in phenotypic tests and hsp65 PCR restriction analysis. DESIGN: We applied: (i) sequencing analysis of four conserved gene targets: 16S rRNA, rpoB, hsp65, and sodA; (ii) two commercial reverse hybridization assays; (iii) protein analysis by matrix assisted laser desorption\/ionization time of flight mass spectrometry  (MALDI-TOF MS).  RESULTS: Combined, but not individual, sequence analysis allowed reliable species identification for 30\/40 (75%) isolates, including species previously unknown to be circulating in Argentina. Commercial kits outperformed our identification routine in only 5\/35 isolates and misclassified many more. MALDI-TOF MS accurately identified species for 22\/36 (61%) isolates and did not misidentify any. CONCLUSIONS: Commercial kits did not resolve species for NTM isolates eluding identification. Combined DNA sequence analysis was the choice approach. MALDI-TOF MS shows promise as a powerful, rapid and accessible tool to identify clinically relevant NTM in the diagnostic laboratory and its accuracy can be maximized by building up a customized NTM spectrum database.","palabras_clave":"MYCOBACTERIOSIS|DNA SEQUENCE ANALYSIS|MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY|DNA PROBES|NUCLEIC ACID HYBRIDIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6041,"idioma_id":4,"titulo":"Operando Raman spectroscopic studies of lithium zirconates during CO2 capture at high temperature","resumen":"Li2ZrO3 based sorbents were synthesized for CO2 capture at high temperature between 500 and 700 C. Monoclinic ZrO2, tetragonal Li2ZrO3, Li2 CO3, K2CO3 and LiKCO3 phases were detected by XRD and Raman spectroscopy. The sorbents showed high stability and moderate capture efficiency. The addition of K resulted in the improvement of capture efficiency from 0.052 g CO2 g mat1 in materials without K to 0.083 g CO2 g mat1 in the doped solid. Here, we report an  experimental setup of Raman spectroscopy coupled with online mass spectrometry and a demonstration of its capabilities using lithium zirconates as sorbents for high temperature CO2 capture. Operando Raman spectroscopy allowed us to follow up the phase evolution during the capture process for the first time. The results obtained confirmed the presence of molten K and Li carbonates when the K-doped zirconates wereexposed to CO2 at 500 C.","palabras_clave":"CAPTURE|CO2|RAMAN|LIZRO3"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6042,"idioma_id":4,"titulo":"Synthesis of docosahexaenoic acid from eicosapentaenoic acid in retina neurons protects photoreceptors from oxidative stress","resumen":"Oxidative stress is involved in activating photoreceptor death in several retinal degenerations. Docosahexaenoic acid (DHA), the major polyunsaturated fatty acid in the retina, protects cultured retina photoreceptors from apoptosis induced by oxidative stress and promotes photoreceptor differentiation. Here we investigated whether eicosapentaenoic acid (EPA), a metabolic precursor to DHA, had similar effects and whether retinal neurons could metabolize EPA to DHA. Adding EPA to rat retina neuronal cultures increased opsin expression and protected photoreceptors from apoptosis induced by the oxidants paraquat (PQ) and hydrogen peroxide (H2O2). Palmitic, oleic, and arachidonic acids had no protective effect, showing the specificity for DHA. We found that EPA supplementation significantly increased DHA percentage in retinal neurons, but not EPA percentage. Photoreceptors and glial cells expressed E6 desaturase (FADS2), which introduces the last double bond in DHA biosynthetic pathway. Pre-treatment of neuronal cultures with CP-24879 hydrochloride, a E5\/E6 desaturase inhibitor, prevented EPA-induced increase in DHA percentage and completely blocked EPA protection and its effect on photoreceptor differentiation. These results suggest that EPA promoted photoreceptor differentiation and rescued photoreceptors from oxidative stress-induced apoptosis through its elongation and desaturation to DHA. Our data show, for the first time, that isolated retinal neurons can synthesize DHA in culture.","palabras_clave":"POLIINSATURATED LIPID ACID|PHOTORECEPTOR|SURVIVAL|DIFFERENCIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6043,"idioma_id":4,"titulo":"Sulfonic functionalized SBA-15 catalysts in the gas phase glycerol dehydration. Thermal stability and catalyst deactivation","resumen":"The catalytic conversion of glycerol to acrolein was studied using sulfonic functionalized mesoporous silica SBA-15. The catalysts were prepared by the co-condensation method, conducting the silica synthesis and functionalization of its surface with thiol-propyl groups in the same stage. In a next step, the oxidation of thiol groups to sulfonic groups was made varying the concentration of H2O2 in order to obtain catalysts with different levels of acidity on the surface. The solids were characterized by BET, SEM, FTIR, XPS, and potentiometric titration with n-butilamine. The thermal stability was analyzed by thermogravimetry (TGA) and temperature-programmed desorption (TPD). The reaction tests were performed at different temperatures between 275 C and 350 C, and under feed flow conditions that allowed the study of the deactivation phenomena by coke deposition. The best temperature in order to maximize theacrolein yield was 300 C. Higher temperatures led to a rapid deactivation by a combined effect of coke deposition and decomposition of functional groups.","palabras_clave":"GLYCEROL|DEHYDRATION|SULFONIC SBA-15|DEACTIVATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6044,"idioma_id":4,"titulo":"WHEY PERMEATE FERMENTED WITH KEFIR GRAINS SHOWS ANTIFUNGAL EFFECT AGAINST FUSARIUM GRAMINEARUM","resumen":"The objective of the work reported here was to study the antifungal capability of cell-free supernatants obtained from whey permeates after fermentation by the kefir grains CIDCA AGK1 against Fusarium graminearum growth and zearalenone (ZEA) production. The assays were performed in order to study the conidial germination inhibition -in liquid media- and the effect on fungal growth rate and the Latency phase -in solid media. We observed that fermented supernatants of pH 3·5 produced the highest percentages of inhibition of conidial germination. The dilution and, particularly, alkalinisation of them led to the gradual loss of antifungal activity. In the fungal inhibition assays on plates we found that only the highest proportion of supernatant within solid medium had significant antifungal activity, which was determined as fungicidal. There was no ZEA biosynthesis in the medium with the highest proportion of supernatant, whereas at lower concentrations, the mycotoxin production was strain-dependent. From the results obtained we concluded that kefir supernatants had antifungal activity on the F. graminearum strains investigated and inhibited mycotoxin production as well, but in a strain-dependent fashion. The present work constitutes the first report of the effect of the products obtained from the kefir-grain fermentation of whey permeates ? a readily available by-product of the dairy industry ? on F. graminearum germination, growth, and toxin production.","palabras_clave":"WHEY PERMEATE|FUSARIUM GRAMINEARUM|FUNGAL INHIBITION|ZEARALENONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6045,"idioma_id":4,"titulo":"NK cells restrain spontaneous anti-tumor CD8+ T cell priming through PD-1\/PD-L1 interactions with dendritic cells","resumen":"Despite the classical function of natural killer (NK) cells in the elimination of tumor and of virus-infected cells, evidence for a regulatory role for NK cells has been emerging in different models of autoimmunity, transplantation, and viral infections.  However, this role has not been fully explored in the context of a growing tumor.  Here we show that NK cells can limit spontaneous cross-priming of tumor antigen-specific CD8+ T cells, leading to reduced memory responses.  After challenge with MC57 cells transduced to express the model antigen SIY (MC57.SIY), NK cell-depleted mice exhibited a significantly higher frequency of SIY-specific CD8+T cells, with enhanced IFN-&#947; production and cytotoxic capability.  Depletion of NK cells resulted in a CD8+ T cell population skewed towards an effector-memory T phenotype which was associated with enhanced recall responses and delayed tumor growth after a secondary tumor challenge with B16.SIY cells.  Dendritic cells (DC) from NK cell-depleted tumor-bearing mice exhibited a more mature phenotype.  Interestingly, tumor-infiltrating and tumor-draining lymph node NK cells displayed an up-regulated expression of the inhibitory molecule PD-L1 that, through interaction with PD-1 expressed on DC, limited DC activation, explaining their reduced ability to induce tumor-specific CD8+ T cell priming.  Our results suggest that NK cells can, in certain contexts, have an inhibitory effect on anti-tumor immunity, a finding with implications for immunotherapy in the clinic.","palabras_clave":"NK CELLS|TUMOR IMMUNITY|CD8 T CELLS|PD-1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6046,"idioma_id":4,"titulo":"Chemical and nutritional properties of different fractions of Prosopis alba pods and seeds","resumen":"The objective of this work was to study chemical and nutritional aspects of different fractions of Prosopis alba. Flours from whole pod, pericarp (pulp) and seeds were obtained. Polyphenols were mainly located in pulp but antioxidant activity was higher in whole pod flour and seeds. In seeds, the fraction with the highest polyphenols and antioxidant activity was the seed coat or testa. Protein content was higher in whole podflour (5.81%) than in pulp flour (3.52%), presenting the seed an appreciable amount 33.6%. These proteins were composed by monomer subunits of 85, 67, 38, 16 and 14 kDa and no prolamins and anti-tryptic activity were detected. Prosopis alba flours presented high content of soluble sugars, mainly composed by sucrose, and also high amount of insoluble dietary fiber. The 27 major mineral was potassium. The whole pod, due to the contribution of seeds, contained high amount of calcium, magnesium, iron and zinc, all indispensable minerals for human nutrition. Therefore, Prosopis alba flours, mainly containing the seeds, constitute nutritional ingredients for bakery and gluten free products.","palabras_clave":"Prosopis alba pods|Prosopis alba seeds|protein profile|mineral composition|nutritional value"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6047,"idioma_id":4,"titulo":"Non-covalent functionalization of multi-walled carbon nanotubes with cytochrome c: Enhanced direct electron transfer and analytical applications","resumen":"This work reports the non-covalent functionalization (dispersion) of multi-walled carbon nanotubes (MWCNTs) with cytochrome c (Cyt c), the direct electron transfer (DET) after drop-coating deposition of MWCNTs-Cyt c dispersion on glassy carbon electrodes (GCE), and the analytical applications for the highly sensitive quantification of hydrogen peroxide. The dispersion and the resulting modified electrodes were studied by UV?visible spectroscopy, scanning electron microscopy, and electrochemical techniques. The drastic treatment for dispersing the MWCNTs (5.0 min sonication in water with ultrasonic tip) produces a partial denaturation that facilitates the interaction of Cyt c with the CNTs and makes possible an efficient electron transfer between the heme group and the electrode. A critical analysis of the influence of different experimental conditions on the efficiency of the dispersion and on the performance of GCE modified with MWCNTs-Cyt c dispersion is also reported. The analytical parameters obtained with GCE\/MWCNTs-Cyt c for the amperometric quantification of hydrogen peroxide at &#8722;0.100 V were: sensitivity of (43 ± 1) mA M&#8722;1 cm&#8722;2, linear range between 1.0 × 10&#8722;6 and 1.6 × 10&#8722;4 M, detection limit of 1.5 × 10&#8722;7 M, reproducibility of 3.1% and repeatability of 3.4%. The biosensor was successfully used for the quantification of hydrogen peroxide in mouthwash and spiked milk samples.","palabras_clave":"MULTI-WALLED CARBON NANOTUBES|CYTOCHROME C|CNT FUNCTIONALIZATION|HYDROGEN PEROXIDE|ELECTROCHEMICAL BIOSENSOR|DIRECT ELECTRON TRANSFER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6048,"idioma_id":4,"titulo":"Isolation of the symbiotic fungus of Acromyrmex pubescens and phylogeny of Leucoagaricus gongylophorus from leaf-cutting ants","resumen":"Leaf-cutting ants live in an obligate symbiosis with a Leucoagaricus species, a basidiomycete that serves as a food source to the larvae and queen. The aim of this work was to isolate, identify and complete the phylogenetic study of L.gongylophorus species of Acromyrmex pubescens. Macroscopic and microscopic features were used to identify the fungal symbiont of the ants. The ITS1-5,8S-ITS2 region was used as molecular marker for the molecular identification and to evaluate the phylogeny within the Leucoagaricus genus. One fungal symbiont associated to Acromyrmex pubescens was isolated and identified as Leucoagaricus gongylophorus. The phylogeny of Leucoagaricus obtained using the ITS molecular marker revealed three well established monophyletic groups. It was possible to recognize one clade of Leucoagaricus associated to phylogenetically derive leaf-cutting ants (Acromyrmex and Atta). A second clade of free living forms of Leucoagaricus (non-cultivated), and a third clade of Leucoagaricus associated tophylogenetically basal genera of ants were also recognized. The clades corresponded to traditional taxonomic groups, and were differentiated by ecological habitats of different species.","palabras_clave":"ITS|LEAF-CUTTING ANTS|LEUCOAGARICUS |PHYLOGENY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6049,"idioma_id":4,"titulo":"When a general morphology allows many habitat uses","resumen":"Evolution of associations between morphology and ecology is evident in several Squamate lineages. Locomotion is an essential aspect that underlies the associations so far identified, representing a major link between the phenotype of an organism and its ecology. Recently, the morphological basis for functional morphology has been revitalized and more detailed data on the internal anatomy are being incorporated for a better understanding of the actual features involved in locomotion. Here we focus on two lizard families, Tropiduridae and Liolaemidae, and use information of muscle-tendinous and external morphology traits of hind legs to investigate whether a) the value of the traits analyzed tend to exhibit a reduced phenotypic variation produced by stabilizing selection; and b) species showing specialization in their habitat use will also exhibit special morphological features related to it. As a result, we identified that evolution of hind limb traits and of muscle-tendinous variables is mainly explained by the Ornstein-Uhlenbeck model, suggesting stabilizing selection. Liolaemids and tropidurids show clear ecomorphological trends in the variables considered, with sand lizards presenting the most specialized morphological traits. Some ecomorphological trends differ between the two lineages, and traits of internal morphology tend to be more flexible than those of external morphology, restricting the ability to identify ecomorphs shared between these two lineages. Conservative traits of external morphology likely explain such restriction, as ecomorphs have been historically defined in other lizard clades based on variation of external morphology","palabras_clave":"EXTERNAL MORPHOLOGY|LIOALEMIDAE|MUSCLES|TENDON|TROPIDURIDAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6051,"idioma_id":4,"titulo":"DERMATAN SULFATE\/CHITOSAN POLYELECTROLYTE COMPLEX WITH POTENTIAL APPLICATION IN THE TREATMENT AND DIAGNOSIS OF VASCULAR DISEASE","resumen":"Cardiovascular disease is the largest single cause of morbid-mortality in the world. However, there is still no pharmaceutical treatment that directly targets the blood vessel wall instead of just controlling the risk factors. Here, we produced polyelectrolyte complexes (PECs) by a simple and reproducible polyelectrolyte complexation method between low molecular mass dermatan sulfate (polyanionic polysaccharide) and chitosan (polycationic polysaccharide), and evaluated the cellular uptake by vascular endothelial cells. The composition and the composition homogeneity of PECs were con&#64257;rmed by 13C-CP-MAS spec-troscopy and by polyacrylamide gel electrophoresis, respectively. The hydrodynamic radius, determined by dynamic light scattering, was 729 ± 11 nm. PECs were not cytotoxic for a murine heart endothelium-derived cell line. Fluorescent confocal microscopy showed the speci&#64257;c uptake of &#64258;uorescently-labeled PECs by endothelial cells when they were cultured alone or in the presence of macrophages. Overall, these &#64257;ndings con&#64257;rmed the potential of these PECs for targeting different agents to the vessel wall in the prevention, diagnosis, and therapy of vascular disease.","palabras_clave":"LOW MOLECULAR MASS DERMATAN SULFATE|CHITOSAN|POLYELECTROLYTE COMPLEXES|ENDOTHELIAL UPTAKE|VASCULAR DISEASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6053,"idioma_id":5,"titulo":"?\tEstudio sobre alfabetización académica en la Facultad de Ciencias Veterinarias de la Universidad Nacional de La Plata","resumen":"Cuando nos referimos a alfabetización académica, hacemos referencia a los conocimientos necesarios para aprender en la universidad. Habiéndose realizado previamente una evaluación diagnóstica en el curso de Patología General Veterinaria, se estudió esta problemática en los cursos de segundo año de la carrera de Ciencias Veterinarias de la Facultad de Ciencias Veterinarias de la UNLP. Los objetivos del estudio incluyeron conocer qué importancia se da en los cursos de segundo año a la lectura y a la escritura, indagar en los supuestos docentes al respecto, analizar las actividades de lectoescritura propuestas a los estudiantes, y conocer las dificultades que éstos enfrentan ante la lectura y escritura de textos académicos. Para ello se analizaron las planificaciones y evaluaciones curriculares de cada curso, relatos de clases y textos escritos por estudiantes. Se realizaron observaciones de clase y se encuestó a docentes y alumnos de segundo año. Los resultados muestran que actividades de lectura y escritura están presentes tanto en el curriculum práctico como en el formal, hallándose las actividades de escritura preponderantemente en la instancia de evaluación. Las encuestas docentes revelan supuestos en transición entre la postura que no reconoce responsabilidad de la universidad en la alfabetización académica y la que sí lo hace. Las encuestas a estudiantes revelaron múltiples dificultades en la lectura, mientras que el análisis de sus escritos mostró dificultades en la escritura. El conocimiento de la situación actual permitirá diseñar proyectos de intervención tendientes a mejorarla, facilitando el proceso de incorporación de los estudiantes a la cultura académica.","palabras_clave":"ALFABETIZACIÓN ACADÉMICA|LECTURA |ESCRITURA|MEDICINA VETERINARIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6054,"idioma_id":5,"titulo":"La constitución de la Argentina democrática en el discurso de asunción presidencial de Raúl Alfonsín","resumen":"Desde la perspectiva de la retórica constitutiva, este artículo estudia el discurso de asunción del presidente argentino Raúl Alfonsín ante el Congreso de la Nación (10 de diciembre de 1983). Con herramientas tomadas de la retórica y del análisis del discurso, estudia cómo esta alocución constituye la democracia después de siete años de dictadura (1976-1983), performando un auditorio heterogéneo, compuesto por diferentes filiaciones políticas e ideológicas, en un sujeto democrático unido y plural, aglutinado en torno a una ética cívica.","palabras_clave":"RETÓRICA CONSTITUTIVA|DISCURSO DE ASUNCIÓN|RAUL ALFONSÍN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6055,"idioma_id":5,"titulo":"Los Glyptodontidae (Mammalia, Xenarthra): Historia biogeográfica y evolutiva de un grupo particular de mamíferos acorazados","resumen":"Los Glyptodontidae constituyeron uno de los clados de Cingulata (Mammalia, Xenarthra) con mayor éxito de América del Sur. Durante su extensa historia evolutiva (Eoceno tardío-Holoceno temprano), es posible observar dos etapas claramente diferenciables. En su etapa inicial (Eoceno tardío-Oligoceno), todos los registros están circunscriptos al sur de América del Sur, desde la región Patagónica hasta Bolivia. Su diversidad está limitada a las subfamilias Glyptatelinae y Propalaehoplophorinae y se conocen solo a partir de fragmentos de la coraza dorsal. Ya durante el Neógeno y Cuaternario se observa una progresiva e importante diversificación y ampliación en la distribución latitudinal de los Glyptodontidae. A lo largo de toda su historia evolutiva, la mayor diversidad de gliptodontes estuvo siempre centrada en ámbitos australes, donde el grupo muestra una importante diferenciación taxonómica con respecto a las formas del norte de América del Sur. En este escenario resulta posible que el mega-humedal ?Pebas? (Mioceno medio) haya jugado un importante papel en el aislamiento y posterior evolución independiente de la diversidad de gliptodontes del norte y sur de América del Sur.","palabras_clave":"Glyptodontidae| América del Sur|Diversidad|Evolución"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6056,"idioma_id":4,"titulo":"Structural and Functional Analysis of Aspergillus Niger Xylanase to Be Employed in Polyethylenglycol\/Salt Aqueous Two-Phase Extraction","resumen":"The structure and enzymatic activity of Aspergillus niger xylanase were evaluated in different media to establish an appropriate protocol for the extraction of the enzyme in polymer\/salt aqueous two-phase systems. Different factors were studied: the concentration and molecular weight (1000, 2000, 4600 and 8000) of polyethyleneglycol, the concentration and type of salt (sodium citrate and potassium phosphate) and pH, time and temperature. Xylanase was stable for 5 hours at pH between 2.7 and 9.0 and at temperatures up to 50ºC. Fluorescence spectroscopy and circular dichroism experiments showed that neither the secondary\/tertiarystructure of the enzyme nor its catalytic activity were significantly altered in the presence of either salt or PEG. Xylanase partitioned into the PEG-rich phase driven by the excluded volume effect. Partitioning was more favorable to the polymer phase in the PEG1000\/NaCit system, where Kp was 12 times higher than in the others aqueous two-phase systems. These results demonstrate the potential application of the PEG1000\/NaCit system as a first step for the extraction of Aspergillus niger xylanase.","palabras_clave":"XYLANASE|ASPERGILLUS NIGER|AQUEOUS TWO-PHASE EXTRACTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6057,"idioma_id":4,"titulo":"A SUSTAINABLE AFFINITY PARTITIONING PROCESS TO RECOVER PAPAIN FROM CARICA PAPAYA LATEX USING ALGINATE AS MACRO-LIGAND","resumen":"The role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex.In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven.When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w\/w) into thesystem doubled the PAP partition coefficient (Kp, PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG\/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integrationof the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts.","palabras_clave":"ALGINATE|PAPAIN|RECOVERY|PAPAYA LATEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6058,"idioma_id":4,"titulo":"Southern right whale (Eubalaena australis) calf mortality at  Península Valdés,  Argentina: are harmful algal blooms to blame?","resumen":"Pen&#305;nsula Valdes (PV) in Argentina is an important calving ground for southernright whales (SRWs, Eubalaena australis). Since 2005, right whale mortality hasincreased at PV, with most of the deaths (~90%) being calves <3mo old. We investigated the potential involvement of harmful algal blooms (HABs) in these deaths by examining data that include: timing of the SRW deaths, biotoxins in samples from dead SRWs, abundances of the diatom, Pseudo-nitzschia spp., and the dinoflagellate, Alexandrium tamarense, shellfish harvesting closure dates, seasonal availability of whale prey at PV and satellite chlorophyll data. Evidence of the whales? exposure to HAB toxins includes trace levels of paralytic shellfish toxins (PSTs) and domoic acid (DA) in tissues of some dead whales, and fragments of Pseudo-nitzschia spp. frustules in whale feces. Additionally, whales are present at PV during both closures of the shellfish industry (due to high levels of PSTs) and periods with high levels ofPseudo-nitzschia spp. and A. tamarense. There is a positive statistical relationship between monthly Pseudo-nitzschia densities (but not A. tamarense) and calf deaths in both gulfs of PV.","palabras_clave":"southern right whale|calf mortality|Península Valdés|harmful algae bloom |toxins"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6059,"idioma_id":5,"titulo":"Síndrome Pulmonar por Hantavirus en Buenos Aires, 2009-2014","resumen":"El virus Andes es el agente causante del síndrome pulmonar por hantavirus en Argentina y países limítrofes. Existen varios genotipos característicos en las cuatro regiones endémicas del país. En la provincia de Buenos Aires, zona central, co-circulan tres genotipos: AND-BsAs, AND-Lechiguanas y AND-Plata.A partir de los casos confirmados por ELISA y PCR en tiempo real durante el período 2009-2014 en Buenos Aires, se evaluó la distribución anual, la letalidad y la distribución geográfica dentro de la provincia; mediante RT-PCR y secuenciación nucleotídica se analizaron los genotipos implicados. También se estudió la evidencia epidemiológica para determinar la ruta de infección en casos agrupados. Se analizaron 1386 muestras de casos sospechosos de la provincia de Buenos Aires, confirmándose 88 casos de Síndrome Pulmonar por Hantavirus durante el período 2009-2014. El promedio general fue de 14.3 casos por año. El análisis del genotipo viral se realizó en el 30.7% de los casos confirmados, AND-BsAs fue el de mayor incidencia, caracterizado en el 72% delos casos estudiados. Se confirmó la ocurrencia de un brote de síndrome pulmonar por hantavirus Buenos Aires durante el año 2013, con un registro de casos 3 veces mayor respecto al promedio anual del período 2009-2012, con tendencia a normalizarse durante 2014. La letalidad general fue del 25.6%, con un valor máximo de 45.5% en 2011. Se evaluaron los datos epidemiológicos y los resultados obtenidos del análisis de comparación de genomasvirales en 3 agrupamientos de 2 casos cada uno, sugiriendo fuertemente transmisión persona a persona.","palabras_clave":"HANTAVIRUS|BUENOS AIRES|BROTE|TRANSMISION INTERHUMANA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6060,"idioma_id":5,"titulo":"Caracterización química y funcional de bebidas formuladas a base de Ficus carica y Clinopodium gillesii","resumen":"Se evalúa el poder antioxidante de bebidas formuladas a partir de frutos de Ficus carica aditivadas con distintas concentraciones de un extracto polar de la planta aromática medicinal nativa Clinopodium gillesii. Se midió la actividad antirradicalaria (AAR) frente al DPPH? y al NO?; así como el contenido de fenoles totales (FT), de antocianinas monoméricas totales (AMT) y de ácido ascórbico. Además fueron determinados color, ºBrix, pH y sólidos solubles totales. Todas las muestras presentaron AAR frente al radical DPPH?, y en menor medida frente al NO? El agregado del extracto de C. gillesii representó un incremento promedio del 25% en la AAR frente a NO?, respecto a la bebida no aditivada, mientras que para el DPPH?, el incremento fue del 38%. El contenido de AMT en la bebida disminuyó con el agregado del extracto, por tanto las AMT no jugarían un rol determinante en dicha AAR .Se observaron correlaciones positivas entre la concentración del extracto activo, los FT y la actividad antirradicalaria de las bebidas formuladas, lo que probaria que el incremento del valor biológico antioxidante de la bebida a base de Ficus carica, es debida a la adición del extracto activo de C. gillesii.","palabras_clave":"Ficus carica|actividad antirradicalaria|Clinopodium gillesii|bebida functional"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6061,"idioma_id":4,"titulo":"Object approach computation by a giant neuron and its relation with the speed of escape in the crab Neohelice","resumen":"Upon detection of an approaching objectthe crab Neohelice granulata continuously regulates thedirection and speed of escape according to ongoing visual information. Thesevisuomotor transformations are thought to be largely accounted for by a smallnumber of motion-sensitive giant neurons projecting from the lobula (thirdoptic neuropil) towards the supraesophageal ganglion. One of these elements,the monostratified lobula giant neurons of type 2 (MLG2), proved to be highlysensitive to looming stimuli (a 2D representation of an object approach).&nbsp;By performing in vivo intracellularrecordings we assessed the response of the MLG2 neuron to a variety of loomingstimuli representing objects of different sizes and velocities ofapproach.&nbsp;This allowed us: a) to identify some of the physiologicalmechanisms involved in the regulation of the MLG2 activity and to test asimplified biophysical model of its response to looming stimuli; b) to identifythe stimulus optical parameters encoded by the MLG2, and to formulate aphenomenological model able to predict the temporal course of the neural firingresponses to all looming stimuli;&nbsp;c) to incorporate the MLG2 encodedinformation of the stimulus (in terms of firing rate) into a mathematical modelable to fit the speed of the escape run of the animal. The agreement betweenthe model predictions and the actual escape speed measured on a treadmill forall tested stimuli strengthens our interpretation of the computations performedby the MLG2 and of the involvement of this neuron in the regulation of theanimal´s speed of run while escaping from approaching objects.&nbsp;","palabras_clave":"Vision|Collision Avoidance|Behaviour|Crab"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6063,"idioma_id":4,"titulo":"Defoliation  Frequency  Effects  on  Shoots  and  Roots  of  Poa ligularis in Arid Patagonia, Argentina","resumen":"Poa ligularis is an important forage, perennial bunchgrass in Patagonian rangelands. We hypothesized that (1) root biomass in  P. ligularis  is not reduced by increased defoliation frequency, as carbon would be  allocated similarly above- (for  leaf and  shoot regrowth) and  belowground (to  maintain the water uptake  necessary for shoot regrowth), and (2) root survival is greater with increasing soil depth in the various defoliation treatments because  shallow  soils  are  characteristically  more  water-limited  depending  on  seasonal  precipitation.  Five defoliation frequencies (plants defoliated once, twice, or three, four or five times, plus 1 non-defoliated control) were applied in a representative grassland of the Occidental District in Patagonia during each of two consecutive growing seasons. Plants were defoliated, leaving 5 cm stubble, whenever regrowth reached 10 cm height. Root disappearance  and  appearance  (using  a  root  color  scale),  and  root  spatial  distribution  in  the  soil,  were determined every 3  cm-soil depth increments up  to 30 cm  in the soil profile using a root periscope. The root color scale allowed us to develop equations to calculate  root increase  and mortality  rates during  each study period. Shoot biomasses  per plant were  not affected by any  defoliation frequency during 2002\/03. However, plants defoliated three or more times annually showed a lower shoot biomass than the non-defoliated controls during the  second  year  of successive  defoliations. Root  dynamics  was not  affected  by the  study defoliation frequencies. Both hypotheses were confirmed by the results. Our  findings suggest  that (1)  plant vigor  on P. ligularis  is  most  often  favored  by  no  more  than  two  defoliations  annually,  immediately  before  internode elongation to 5 cm stubble height each, and (2) root field studies should be performed using short- rather than long-sampling increments into the soil profile because  changes in root dynamics occurred at a scale of a  few centimeters within it.","palabras_clave":"Perennial grasses|Root dynamics|Root mortality|Root recruitment"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6064,"idioma_id":4,"titulo":"Influence of particle size on the adsorption and release of cephalexin encapsulated in mesoporous silica SBA-15","resumen":"Ordered mesoporous silicas withdifferent particle sizes were synthesized and used as drug carrier in controlleddelivery systems. Morphological properties of mesoporous supports were modifiedby changing the stirring rate. Changes in the shape of SBA-15 fromfiber-like particlesto smaller irregular agglomerates were obtained by reducing the stirring rate. Theadsorption and release of cephalexin from those different supports wereinvestigated. Adsorption capacity was found to increase with decreasing particlesizes, and the in vitro release experiments indicated a clear increase in theamount released from the smaller particles. In vivo studies revealed thatsilica matrixes effectively control the drug release and were capable ofmaintaining a sustained therapeutic blood level of cephalexin.&nbsp;&nbsp;&nbsp;","palabras_clave":"Particle size|Adsorption|Drug delivery|Cephalexin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6065,"idioma_id":4,"titulo":"Taxonomy and cladistics of the group of genera related to Cyrtomon Schoenherr (Coleoptera: Curculionidae: Naupactini)","resumen":"In this contribution we update taxonomic and geographical information on five genera of weevils (Curculionidae: Entiminae: Naupactini) distributed in Argentina, Brazil, Bolivia, Paraguay and Uruguay.  As a result of a cladistic analysis based on 48 morphological characters and 18 terminal species (two outgroups and 16 species of the ingroup) we recover a single most parsimonious tree with two main subgroups, one including Mendozella Hustache as a sister group of Cyrtomon Schoenherr, and the other including Lamprocyphopsis Lanteri, Priocyphopsis Lanteri and Priocyphus Hustache.  In this tree Cyrtomon hirsutus (Hustache) is the sister species of Mendozella  curvispinis (Hustache), consequently we establish the new combination Mendozella hirsuta (Hustache), and herein consider that the genus Mendozella includes two species, both from Mendoza province, Argentina. We confirm the monophyly of Cyrtomon, which includes six species, C. gibber (Pallas), C. pistor (Boheman), C. luridus (Boheman), C. inhalatus (Germar), C. ovalipennis (Hustache) and C. glaucus (Bovie).  Priocyphus is monophyletic and includes  five species, P. bosqi Hustache, P. hustachei Kuschel, P. inops Kuschel, P. kuscheli Lanteri and the new species Priocyphus cordobensis herein described; Lamprocyphopsis  includes two species, L. viridinitens (Kuschel) and L. paraguayensis Lanteri; and Priocyphopsis is monotypic, with the only species P. humeridens (Hustache) recovered as sister group of Priocyphus. We provide diagnosis of all the genera, a key and habitus photographs of all the species, most of them corresponding to type specimens. Diagnostic characters of rostrum- heads, front tibiae and genitalia are illustrated with line drawings. The female genitalia of Mendozella hirsuta and the male genitalia of Priocyphus kuscheli are described for the first time.","palabras_clave":"América del Sur|Distribucion Geográfica|Cyrtomon|Naupactini"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6066,"idioma_id":4,"titulo":"Anatomical and systematic study of proximal tarsals of ungulates from Geste Formation (Northwestern Argentina)","resumen":"In the last decades, Paleogene outcrops in northwestern Argentina have provided abundant information about the evolution of South American mammals during the early Cenozoic. This region constituted an important area of early diversification for several groups of native ungulates during the Eocene, especially notoungulates. To date, most reports describe cranial material, even when in many cases specimens are found in association with postcranial elements. It is well known that these elements can provide characters for phylogenetic analyses and supply important clues for paleobiological inferences. This study presents a series of postcranial remains (proximal tarsals) from the Geste Formation, which crops out at Antofagasta de la Sierra, Catamarca Province, Argentina. Three calcanei and two astragali are described, compared with those of extinct ungulates, and systematically determined. A functional analysis allows inferring the association of these bones with different types of stances. One calcaneus is assigned to Toxodontidae (Notoungulata) and exhibits some plantigrade features. The other two calcanei and an incomplete astragalus are referred to Interatheriidae (Notoungulata), showing digitigrade-like morphology. Another astragalus is referred to Didolontidae Ernestokokenia cf. yirunhor?; its configuration suggesting a digitigrade posture. The study of the tarsal provides, as well, a more integrative view of the Paleogene forms of the region.","palabras_clave":"Postcranial|Eocene|Catamarca|Calcaneum"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6067,"idioma_id":4,"titulo":"An index to evaluate the fluvial habitat degradation in lowland urban streams","resumen":"The objective of the present article is to propose an index that allows the assessment of the fluvial habitat quality in lowlandstreams that run through urbanized areas, by the use of metrics relatedto the quality of the watercourse, the river banks, the riparian zone andthe fluvial geomorphology. The metrics retained in the index (USHI, UrbanStream Habitat Index) include the cover percentage and quality of theaquatic vegetation; the main features of the river banks; the presence ofexotic trees, of litter, of permanent structures (such as buildings) inthe riparian zone and other major geomorphological alterations, such asdredging or channelization. The index is related to physical-chemicalparameters that are linked to water quality, the imperviousness of thewatershed and to other biotic descriptors, particularly themacroinvertebrate and diatom assemblages. The values of the index for thesites in the studied area revealed that 41.2% have a bad or very badhabitat quality, 27.8% a moderate habitat quality, while 31% have a goodor very good habitat quality. The main issue detected in the studiedsites involved the dredging or partial channelization of the reaches.Unlike other indices that evaluate the quality of the physical habitatthrough the use of the diatoms or macroinvertebrate communities, the USHIcan be interpreted as a measure of the overall quality of the habitat,and uses indicators that do not require the identification of taxa,making it more accessible to non-specialists. Therefore it provides witha tool to evaluate the fluvial habitat quality of lowland streams thatcan be easily applied, particularly by professionals that take part inthe management and decision making process regarding urbanizedwatersheds.","palabras_clave":"STREAM HABITAT|URBAN STREAM |MONITORING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6069,"idioma_id":5,"titulo":"La calidad panadera y dinámica del área foliar verde de trigo pan, frente a mancha amarilla y roya de la hoja","resumen":"El trabajo investiga el efecto sobre la calidad panadera de patógenos foliares del trigo como la mancha amarilla y la roya de la hoja","palabras_clave":"TRIGO|CALIDAD PANADERA|ROYA DE LA HOJA|MANCHA AMARILLA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6071,"idioma_id":4,"titulo":"Structural and morphological evolutions of spent FCC catalyst pellets toward NaA zeolite","resumen":"Spherically shaped pellets, usually discarded after use in fluidized-bed catalytic cracking units (FCCs), were converted to zeolite A-surface-enriched pellets. The final pellets were obtained through a two-step treatment consisting of: (1) alkaline thermal activation followed by (2) a hydrothermal crystallization in selected reaction conditions. The alkaline thermal activation provided pellets mainly constituted by silico-aluminate compounds. When the pellets are heated at 800 C in contact with sodium carbonate, a structural rearrangement occurs which includes nepheline, crystalline aluminosilicate, and an amorphous fraction expected to be silicon-enriched, preserving the pellet original geometry.Afterward, reaction mixtures were prepared by adding sodium hydroxidesolution to the heat-treated product. Then, commercial sodium aluminate wasadded. During the hydrothermal synthesis at 85 ± 3 C, zeolite A was formedfrom the calcined product and NaAlSiO4 turned out to be an intermediarycrystalline compound. The LTA crystals were already observed for a reactiontime of 0.5 h, but the highest conversion to pure zeolite A was reached after 6 h.Those final round solid pellets showed an external surface fully covered by wellanchored zeolite A cubic crystals about 1?2 lm in size. Such materials may bevery useful in ion exchange, molecular sieving, and adsorption processes. Thecrystalline ordering was followed by XRD, SEM, NMR, and water adsorption.","palabras_clave":"WASTE FCC CATALYST|ZEOLITE A| POLYMORPHS OF NaAlSiO4"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6072,"idioma_id":4,"titulo":"Nest environment modulates begging behavior of a generalist brood parasite","resumen":"In young birds, the intensity of the begging signal varies according to the chick?s internal condition (i.e., hunger or nutritional state) but may be additionally modulated by the external conditions experienced in the nest (e.g., nestmate competition or feeding success). Flexibility of begging in response to nest environment should be especially beneficial for the chicks of generalist brood parasites, which encounter diverse brood conditions depending on the host nest in which they are reared. We investigated variability in the begging behavior of an extreme generalist brood parasite, the shiny cowbird (Molothrus bonariensis). We compared visual and vocal begging variables of cowbird young of 5 days of age reared in nests of 2 common hosts, one smaller (house wren, Troglodytes aedon) and one larger (chalk-browed mockingbird, Mimus saturninus) than the parasite. Cowbird chicks were standardized for short-term hunger prior to recording and were similar in mass\/condition in each host, but nevertheless begged more intensely in mockingbird than house wren nests, as indicated by increased stretching of the neck, lower latency to beg, increased time spent begging per bout and greater call rate. These results are consistent with young cowbirds adjusting their begging intensity based on the intrabrood competition of a given host, independent of need, an adaptability that likely contributes to their success as parasites utilizing diverse host species.","palabras_clave":"Begging|Brood parasitism|Cowbird|Molothrus bonariensis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6073,"idioma_id":4,"titulo":"Experimental evidence for an antipredatory function of egg rejection behaviour in a common host of the brood-parasitic shiny cowbird","resumen":"The rejection of foreign eggs is the most effective adaptation against brood parasitism in birds. Many hosts, however, show suboptimal responses towards parasitic eggs, which could reflect a compromise between the benefits and costs of egg rejection. Some large-sized hosts of the shiny cowbird (Molothrus bonariensis) accept any spotted parasite egg but reject a rarer pure white egg morph that occurs only in some parts of the parasite?s distribution. This behaviour is intriguing because it is not an effective defense against parasitism and recent evidences suggest that large-sized hosts could benefit from accepting cowbird eggs as this may dilute the risk of host egg losses at multiply parasitized nests. We studied whether rejection of pure white cowbird eggs can be driven by the increased predation risk of host nests parasitized with this conspicuous egg morph. We conducted a nest predation experiment using artificial clutches placed in natural, inactive nests of a large-sized host, the chalk-browed mockingbird (Mimus saturninus). Clutches consisted of two eggs resembling either one host plus one pure white cowbird egg, one host plus one spotted cowbird egg, or two host eggs. Clutches with pure white eggs were more likely to be predated than those having either two host eggs or one host and one spotted cowbird egg, supporting an antipredatory function of egg rejection behaviour in mockingbirds. These results suggest that nest predation could operate as part of larger fitness trade-offs shaping host responses towards foreign eggs. Considering the role of nest predation in the studies of host rejection decisions would help to better understand the evolution and expression of antiparasite defenses, especially when hosts seem to behave suboptimally against costly brood parasitism.","palabras_clave":"brood parasitism|egg rejection|Molothrus|nest predation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6074,"idioma_id":4,"titulo":"Analysis of class 2 integrons as a marker for multidrug resistance among Gram negative bacilli","resumen":"Class 1 and 2 integrons are considered the paradigm of multidrug resistant (MDR) integrons. Although class 1 integrons have been found statistically associated to Enterobacteriaceae MDR isolates, this type of study has not been conducted for class 2 integrons. Escherichia coli and 3 species that were found that harbored more than 20% of class 2 integrons in clinical isolates, were selected to determine the role of intI2 as MDR marker. A total of 234 MDR\/191 susceptible non-epidemiologically related isolates were analyzed. Seventy-four intI2 genes were found by PCR and sequencing. An intI2 relationship with MDR phenotypes in Acinetobacter baumannii and Enterobacter cloacae was found. No statistical association was identified with MDR E. coli and Helicobacter pylori isolates. In other words, the likelihood of finding intI2 is the same in susceptible and in MDR E. coli and H. pylori strains, suggesting a particular affinity between the mobile element Tn7 and some species. The use of intI2 as MDR marker was species-dependent, with fluctuating epidemiology at geographical and temporal gradients. The use of intI2 as MDR marker is advisable in A. baumannii, a species that can reach high frequencies of this genetic element.","palabras_clave":"bacteria|nosocomio|integron"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6075,"idioma_id":5,"titulo":"Viajes, ciudades y exhibiciones: Las modernidades vistas y escritas por Gabriel Carrasco en 1889","resumen":"Este artículo analiza las representaciones de Gabriel Carrasco sobre el viajemoderno, las modernidades urbanas y las exposiciones universales. Representante deuna ciudad puerto argentina con fuertes aspiraciones de modernización, Carrasco fue un agudo observador de las modernidades europeas y sus comparaciones y traducciones a la Argentina. Sus relatos del viaje a la Exposición Universal de París de 1889 constituyen un acceso para el análisis de la modernización técnica de los transportes, la experiencia de la compresión espaciotemporal, la reestructuración de las ciudades con sus principales artefactos modernos, la relevancia de las infraestructuras portuarias y las formas de articulación-exhibición de esas modernidades. El texto propone reconstruir las marcas locales de la grilla que Carrasco construyó para apreciar las modernidades europeas y las contradicciones y desajustes que esas lecturas revelaron sobre la constitución de una perspectiva modernizadora aplicable a las ciudades argentinas.","palabras_clave":"MOVILIDAD|MODERNIDADES|TECNOLOGÍAS|CIUDADES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6076,"idioma_id":5,"titulo":"La construcción escenográfica de la ciudad. Espacio público, turismo e itinerarios en Rosario (Argentina)","resumen":"Las planificaciones son discursos hegemónicos dentro del proceso de producción, reproducción e institucionalización de imágenes de la ciudad y de espacialidades. En este artículo nos interesa establecer cómo definen estos artefactos discursivos al espacio público, cómo presuponen su diseño, de qué forma imaginan a sus destinatarios y cuáles son las expectativas depositadas en las áreas ribereñas de la ciudad. La estrategia metodológica combina el análisis de los imaginarios urbanos a través de las prácticas específicas promovidas sobre el espacio de la ciudad, con un análisis discursivo operado sobre los significantes presentes en los corpus documentales que registran las variaciones en la planificación estratégica y urbana de Rosario (Argentina).","palabras_clave":"planeamiento estratégico|espacio público|turismo|imaginarios urbanos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6077,"idioma_id":4,"titulo":"The  simultaneous  presence  of  glyphosate  and  phosphate  at  the goethite  surface  as  seen  by  XPS,  ATR-FTIR  and  competitive  adsorption isotherms","resumen":"The  simultaneous  adsorption  of  glyphosate  and  phosphate  on  goethite  was  studied  by  combining  macroscopic  (adsorption  isotherms,  electrophoresis)  and  spectroscopic  (XPS,  ATR-FTIR)  techniques.  Adsorptionisotherms  together  with  the  Competitive  Langmuir  isotherm  indirectly  show  that  both  substances  coexist  at  the  goethite  surface.  The  adsorption  maximum  for  phosphate  was  2.50  mol\/m2 whereas  for glyphosate  it  was  1.77  mol\/m2 .  The  Langmuir  adsorption  constant  was  0.15  M&#8722;1 for  phosphate  and0.01  M&#8722;1 for  glyphosate.  The  shape  and  position  of  the  XPS  signals  of  glyphosate  did  not  change  by the  presence  of  phosphate  at  the  surface  and  vice  versa.  Equivalent  results  were  found  with  ATR-FTIR.Therefore,  spectroscopic  evidence  indicates  that  the  binding  mode  of  glyphosate  (type  of  inner-sphere complexes  formed)  to  the  goethite  surface  is  not  modified  by  adsorbing  phosphate.  This  is  valid  for systems  under  equilibrium  conditions  and  under  dynamic  conditions.  The  findings  are  important  in  environmental  modeling,  showing  that  surface  complexation  models  can  be  used  with  confidence  to  predict speciation  in  double-ligand  systems  using  adsorption  parameters  obtained  with  single-ligand  systems.","palabras_clave":"Glyphosate|Phosphate|Competitive  adsorption|Surface  spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6078,"idioma_id":4,"titulo":"Organochlorine pesticides in agricultural soils and associated biota","resumen":"The behavior of organochlorine pesticides (OCPs), such as DDTs, endosulfans, HCHs,heptachlors, drins and chlordanes, have been evaluated in the agricultural watershedof Rio Quequén Grande involving different matrices. Soil profiles from field dedicated tosoybean cultures, associated terrestrial biota (earthworms, coleopteran larvae,collembolan and mites), stream water, suspended particle matter and surface sedimentwere sampled during pre-application pesticide period. Pesticide analyses wereperformed using a gas chromatography with electron capture detector. Soils samplesshowed a differential pesticide pattern along the profile. Control soils, settled 200 mtsfrom agricultural plots presented similar pesticide levels with higher variety ofcompounds, denoting the historical use of OCPs in the zone. However in agriculturalplots DDTs and endosulfans constituted the 90% of pesticides found with anenrichment of endosulfan sulfate with depth. The relative high p,p´-DDT levels insurface agricultural soil would be a consequence of impurities of this pesticide intechnical acaricide Dicofol widely used in the region; and it represent a fresh p,p´-DDTinput in the environment despite its forbidden use. Terrestrial biota, mainly mesofaunademonstrated high capacity to accumulate OCPs, being these organisms a goodoption for monitoring trace pesticides. Moreover the high OCPs levels found constitutea hazard to food web since these organisms are one of the first steps in the food chainand the biomagnification is a common process with these compounds. As a result ofrunoff and volatilization the occurrence of OCPs in aquatic environment was observed,reaching the river a load of 21.5 kg of pesticides daily delivered. As a whole, theseresults suggest the importance of using terrestrial biota for studying recalcitrantpesticides and a continual monitoring is strongly recommended as a first preventivemeasure to minimize environmental risks.","palabras_clave":"PESTICIDAS|SUELO|AGRICULTURA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6079,"idioma_id":5,"titulo":"Efectividad de manejo en reservas naturales urbanas de la Patagonia Austral y recomendaciones para mejorar su gestión turística","resumen":"La efectividad de manejo es un procedimiento metodológico que utiliza indicadores, a los efectos de examinar vacíos existentes y necesidades de mejora en la gestión de las áreas protegidas. Este trabajo presenta su aplicación en seis Reservas Naturales Urbanas de Santa Cruz (Patagonia, Argentina), con la incorporación de un nuevo ámbito de análisis denominado Turismo Responsable, que permite identificar las fortalezas y debilidades en esta actividad y brindar recomendaciones. Los mejores valores fueron los de la reserva Laguna Nimez, ubicada en el centro turístico internacional de El Calafate, y la Reserva Costera Urbana de Río Gallegos, correspondiéndoles un nivel de Manejo Satisfactorio. Ello implica que están cumpliendo con sus objetivos de creación, si bien se requieren algunos ajustes o modificaciones para alcanzar el manejo óptimo. En la gestión turística, L. Nimez presentó la efectividad más elevada (84%), no obstante, se destaca el bajo valor de la variable ?Capacidad de carga?, lo cual indica una de las prioridades a considerar en su manejo, a fin de evitar sobrecargas y mantener en buenas condiciones de conservación al ecosistema","palabras_clave":"Efectividad de manejo|Reservas naturales urbanas|turismo responsable |Patagonia Austral Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6080,"idioma_id":4,"titulo":"SUSTAINED EXOCYTOSIS AFTER ACTION POTENTIAL-LIKE STIMULATION AT LOW FREQUENCIES IN MOUSE CHROMAFFIN CELLS DEPENDS ON A DYNAMIN-DEPENDENT FAST ENDOCYTOTIC PROCESS","resumen":"Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 ± 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (&#964; = 0.53 ± 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies.","palabras_clave":"membrane capacitance|endocytosis|secretion|Ca2+ current"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6081,"idioma_id":4,"titulo":"Determination of tryptamine in foods using square wave adsorptive stripping voltammetry","resumen":"Tryptamine, a biogenic amine, is an indole derivative with an electrophilic substituent at the C3 position of the pyrrole ring of the indole moiety. The electrochemical oxidation of tryptamine was investigated using glassy carbon electrode (GCE), and focusing on trace level determination in food products by square wave adsorptive stripping voltammetry (SWAdSV). The electrochemical responses of tryptamine were evaluated using differing voltammetric techniques over a wide pH range, a quasi-reversible electron-transfer to redox system represented by coupled peaks P1?P3, and an irreversible reaction for peak P2 were demonstrated. The proton and electron counts associated with the oxidation reactions were estimated. The nature of the mass transfer process was predominantly diffusion-limited for the oxidationprocess of P1, the most selective and sensitive analytical response (acetate buffer solution pH 5.3), being used for the development of SWAdSV method, under optimum conditions. The excellent response allowed the development of an electroanalytical method with a linear response range of from 4.7?54.5)108 mol L1, low detection limit (0.8109 mol L1), and quantification limit (2.7 10?9 mol L1), and acceptable levels of repeatability (3.6%), and reproducibility (3.8%). Tryptamine content was determined in bananas, tomatoes, cheese (mozzarella and gorgonzola), and cold meats (chicken sausage and pepperoni sausage), yielding recoveries above 90%, with excellent analytical performance using simple and low cost instrumentation.","palabras_clave":"TRYPTAMINE |ADSORPTIVE |STRIPPING|VOLTAMMETRY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6082,"idioma_id":4,"titulo":"New heterodontosaurid remains from the Cañadón Asfalto Formation: cursoriality and the functional importance of the pes in small heterodontosaurids","resumen":"New ornithischian remains reported here (MPEF-PV 3826) include two complete metatarsi with associated phalanges and caudal vertebrae, from the late Toarcian levels of the Cañadón Asfalto Formation. We conclude that these fossil remains represent a bipedal heterodontosaurid but lack diagnostic characters to identify them at the species level, although they probably represent remains of Manidens condorensis, known from the same locality. Histological features suggest a subadult ontogenetic stage for the individual. A cluster analysis based on pedal measurements identifies similarities of this specimen with heterodontosaurid taxa and the inclusion of the new material in a phylogenetic analysis with expanded character sampling on pedal remains confirms the described specimen as a heterodontosaurid. Finally, uncommon features of the digits (length proportions among nonungual phalanges of digit III, and claw features) are also quantitatively compared to several ornithischians, theropods, and birds, suggesting that this may represent a bipedal cursorial heterodontosaurid with gracile and grasping feet and long digits. In particular, the elongated non-terminal pedal phalanges and morphology of digit III resemble features present in arboreal birds, a unique condition found so far among ornithischians.","palabras_clave":"Jurassic|Ornithischia|Patagonia|Hindlimb"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6083,"idioma_id":4,"titulo":"DEVELOPMENT A HIGH SPECTRAL RESOLUTION LIDAR USING A MULTI-MODE LASER AND A TUNABLE INTERFEROMETER","resumen":"A High Spectral Resolution Lidar (HSRL) using anunseeded laser is designed to separate both Rayleigh and Mie scatteringcomponents by means of a tunable interferometer. The condition is achieved bytuning the interferometer?s free spectral range (FSR) to be the same as themode separation of the laser or a multiple of it. This method has differentcollection efficiency for aerosol and molecular backscatter. We estimate thoseefficiencies for the perfect tuning case as well as when accuracy or precisionerrors on the alignment occurs for a specific simulated condition","palabras_clave":"LIDAR|AEROSOL|HIGH RESOLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6084,"idioma_id":4,"titulo":"A CONCEPT OF MULTI-MODE HIGH SPECTRAL RESOLUTION LIDAR USING MACH-ZEHNDER INTERFEROMETER","resumen":"Inthis paper, we designed a High Spectral Resolution Lidar (HSRL) using a laserthat oscillates in a multi-longitudinal mode. Rayleigh and Mie scatteringcomponents are separated using a Mach-Zehnder Interferometer (MZI) with thesame free spectral range (FSR) as the transmitted laser. The transmitted laser lightis measured as a reference signal with the same MZI. By scanning the MZIperiodically with a scanning range equal to the mode spacing, we can identifythe maximum Mie and the maximum Rayleigh signals using the reference signal.The cross talk due to the spectral width of each laser mode can also beestimated. We present some simulation results related to the selection of thetransmission laser. The optimal laser has shorter resonator length, shorterpulse length, and less beam divergence","palabras_clave":"LIDAR|HIGH RESOLUTION|MACH-ZEHNDER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6085,"idioma_id":4,"titulo":"AEROSOLS MONITORING NETWORK TO CREATE A VOLCANIC ASH RISK MANAGEMENT SYSTEM IN ARGENTINA AND CHILE","resumen":"Two main decisions were made in Argentina tomitigate the impact of the recent volcanic activity in de country basicallyaffected by the presence of volcanic ash in the air and deposited over the Argentineanterritory. The first one was to create a risk management commission were thisrisk between others were studied, and second to develop new ground based remotesensing technologies to be able to identify and inform the risk close to theairports. In addition The Japanese government program for Science andTechnology joint Research Partnership between Argentina, Chile and Japan forSustainable Development (SATREPS) accepted to fund this cooperation due to thepotential future utilization of the research outcomes to the benefit of thesociety. This work present the actual achievements and expected advance ofthese projects that try to joint efforts between national and internationalagencies as well as countries on behalf of a better understanding of the risksand a joint collaboration on the mitigation of suspended ashes impact over theaerial navigation. ","palabras_clave":"SATREPS|VOLCANIC ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6086,"idioma_id":4,"titulo":"Construction and first atmospheric observations of a high spectral resolution lidar system in Argentina in the frame of a trinational Japanese - Argentinean - Chilean collaboration","resumen":"Atmospheric monitoring stations arebeing developed in Argentina. The most important targets are volcanic ashes,desert aerosols in particular Patagonian dust and biomass burning aerosols. Sixstations deployed in the Patagonian Region and Buenos Aires have lidar systems,sun photometers integrated to the AERONET\/NASA monitoring network, in situ opticalparticle analyzers, four solar radiation sensors (pyranometer, UVA, UVB andGUV), and meteorological equipment. The stations are in the main internationalairports of the Regions (San Carlos de Bariloche, Comodoro Rivadavia, Neuquén,Rio Gallegos) and in Buenos Aires (Aeroparque Jorge Newbery and atCEILAP\/CITEDEF). CEILAP and the National Institute of Environmental Studies(NIES) at Tsukuba, Japan developed the first iodine cell based high spectralresolution lidar (HSRL) in Argentina to add in the lidar network. We upgradedthe standard CEILAP multi-wavelength Raman lidar adding the laser frequencytuning system and the 532 iodine-filtered channel at the reception to built theHSRL. HSRL will provide daytime and nighttime direct observation of the aerosoland cloud optical properties (backscatter and extinction) without thepre-assumption of the lidar ratio. This work shows the design and constructionof the first Argentinean HSRL. We also show the first lidar observations donein the country with this kind of lidar.","palabras_clave":"LIDAR|AEROSOL|HSRL|VOLCANIC ASH|SATREPS|SAVER.NET"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6087,"idioma_id":4,"titulo":".Development of a high-spectral-resolution lidar for continuous observation of aerosols in South America","resumen":"Continuousmonitoring of aerosol profiles using lidar is helpful for a quasi-real-timeindication of aerosol concentration. For instance, volcanic ash concentrationand its height distribution are essential information for plane flights. Depolarizationratio and multi-wavelength measurements are useful for characterizing aerosoltypes such as volcanic ash, smoke, dust, sea-salt, and air pollution aerosols.High spectral resolution lidar (HSRL) and Raman scattering lidar can contributeto such aerosol characterization significantly since extinction coefficientscan be measured independently from backscattering coefficients. In particular,HSRL can measure aerosol extinction during daytime and nighttime with a highsensitivity. We developed an HSRL with the iodine filter method for continuousobservation of aerosols at 532nm in the northern region of Argentina in theframework of the South American Environmental Atmospheric Risk ManagementNetwork (SAVER.Net)\/SATREPS project. The laser wavelength of the HSRL wascontrolled by a feedback system to tune the laser wavelength to the center ofan iodine absorption line. The stability of the laser wavelength with thesystem satisfied the requirement showing very small systematic errors in theretrieval of extinction and backscatter.","palabras_clave":"HIGH SPECTRAL RESOLUTION LIDAR|RAMAN LIDAR|AEROSOL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6089,"idioma_id":5,"titulo":"Análisis del confort climático en la ciudad de Tandil, Argentina","resumen":"Los efectos urbanos sobre el clima regional también alteran las condiciones deconfort, modificándolas a escala local y microclimática. No existen trabajos sobreesta temática en la ciudad de Tandil, por lo cual el objetivo del presente artículo esestudiar el confort en la ciudad. Se analizaron las condiciones regionales utilizandotres índices de verano y uno de inverno, ya que se considera que las ciudadescon clima templado generan mayores condiciones de poco confort en verano queen invierno. Los mismos índices fueron calculados para realizar una comparaciónurbano-rural de la temática. Además, se estudió particularmente la distribuciónespacial del Humidex para la estación estival. El resultado fue que la ciudadpresentó mejores condiciones de confort climático durante el invierno que el áreacircundante, mientras que durante la tarde y la noche del verano se presentaronáreas de poca confortabilidad en el norte y en el centro urbano.","palabras_clave":"Clima urbano|Confort climático|Humidex|Tandil"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6090,"idioma_id":4,"titulo":"AR-12 Inhibits Multiple Chaperones Concomitant With Stimulating Autophagosome Formation Collectively Preventing Virus Replication","resumen":"We have recently demonstrated that AR-12 (OSU-03012) reduces the function and ATPase activities of multiple HSP90 and HSP70 family chaperones. Combined knock down of chaperones or AR-12 treatment acted to reduce the expression of virus receptors and essential glucosidase proteins. Combined knock down of chaperones or AR-12 treatment inactivated mTOR and elevated ATG13 S318 phosphorylation concomitant with inducing an endoplasmic reticulum stress response that in an eIF2&#945;-dependent fashion increased Beclin1 and LC3 expression and autophagosome formation. Over-expression of chaperones prevented the reduction in receptor\/glucosidase expression, mTOR inactivation, the ER stress response, and autophagosome formation. AR-12 reduced the reproduction of viruses including Mumps, Influenza, Measles, Junín, Rubella, HIV (wild type and protease resistant), and Ebola, an effect replicated by knock down of multiple chaperone proteins. AR-12-stimulated the co-localization of Influenza, EBV and HIV virus proteins with LC3 in autophagosomes and reduced viral protein association with the chaperones HSP90, HSP70, and GRP78. Knock down of Beclin1 suppressed drug-induced autophagosome formation and reduced the anti-viral protection afforded by AR-12. In an animal model of hemorrhagic fever virus, a transient exposure of animals to low doses of AR-12 doubled animal survival from &#8764;30% to &#8764;60% and suppressed liver damage as measured by ATL, GGT and LDH release. Thus through inhibition of chaperone protein functions; reducing the production, stability and processing of viral proteins; and stimulating autophagosome formation\/viral protein degradation, AR-12 acts as a broad-specificity anti-viral drug in vitro and in vivo. We argue future patient studies with AR-12 are warranted.","palabras_clave":"chaperones|antiviral|AR-12|autophagosomes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6091,"idioma_id":4,"titulo":"Allogeneic Mesenchymal Stromal Cells Overexpressing Mutant Human Hypoxia-Inducible Factor 1-a (HIF1-a) in an Ovine Model of Acute Myocardial Infarction","resumen":"BACKGROUND: Bone marrow mesenchymal stromal cells (BMMSCs) are cardioprotective in acute myocardial infarction (AMI) because of release of paracrine angiogenic and prosurvival factors. Hypoxia-inducible factor 1-á (HIF1-á), rapidly degraded during normoxia, is stabilized during ischemia and upregulates various cardioprotective genes. We hypothesized that BMMSCs engineered to overexpress mutant, oxygen-resistant HIF1-á would confer greater cardioprotection than nontransfected BMMSCs in sheep with AMI.METHODS AND RESULTS: Allogeneic BMMSCs transfected with a minicircle vector encoding mutant HIF1-á (BMMSC-HIF) were injected in the peri-infarct of sheep (n=6) undergoing coronary occlusion. Over 2 months, infarct volume measured by cardiac magnetic resonance (CMR) imaging decreased by 71.7±1.3% (P<0.001), and left ventricular (LV) percent ejection fraction (%EF) increased near 2-fold (P<0.001) in the presence of markedly decreased end-systolic volume. Sheep receiving nontransfected BMMSCs (BMMSC; n=6) displayed less infarct size limitation and percent LVEF improvement, whereas in placebo-treated animals (n=6), neither parameters changed over time. HIF1-á-transfected BMMSCs (BMMSC-HIF) induced angio-\/arteriogenesis and decreased apoptosis by HIF1-mediated overexpression of erythropoietin, inducible nitrous oxide synthase, vascular endothelial growth factor, and angiopoietin-1. Cell tracking using paramagnetic iron nanoparticles in 12 additional sheep revealed enhanced long-term retention of BMMSC-HIF.CONCLUSIONS: Intramyocardial delivery of BMMSC-HIF reduced infarct size and improved LV systolic performance compared to BMMSC, attributed to increased neovascularization and cardioprotective effects induced by HIF1-mediated overexpression of paracrine factors and enhanced retention of injected cells. Given the safety of the minicircle vector and the feasibility of BMMSCs for allogeneic application, this treatment may be potentially useful in the clinic.","palabras_clave":"ANGIOGENESIS|GROWTH FACTORS|MYOCARDIAL INFARCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6092,"idioma_id":4,"titulo":"Polyethylene glycol-coated blue-emitting silicon dots with improved properties for uses in aqueous and biological environments","resumen":"Polyethylene glycol (PEG) grafting to ultrasmall photoluminescent silicon dots (SiDs) is expected to improve and expand the applications of these particles to aqueous environments and biological systems. Herein we report a novel one-pot synthesis of robust, highly water compatible PEG-coated SiDs (denoted as PEG-SiDs) of (3.3 ± 0.5) nm size. The nanoparticles synthesis is based on the liquid phase oxidation of magnesium silicide using PEG as reaction media and leading to high PEG density grafting. PEG-SiDs enhanced photophysical, photosensitizing, and solution properties in aqueous environments are described and compared to those of 2 nm-size PEG-coated SiDs with low PEG density grafting (denoted as PEG-NHSiDs) obtained from a multistep synthesis strategy. PEG-SiDs form highly dispersed suspensions in water showing stable photoluminescence and quantum yields of &#61510; = 0.13 ± 0.04 at 370 nm excitation in air-saturated suspensions. These particles exhibited the capacity of photosensitizing the formation of singlet molecular oxygen, not observed for PEG-NHSiDs. PEG robust shielding of the silicon core luminescent properties is further demonstrated in bio-imaging experiments stressing the strong interaction between PEG-SiDs and S. aureus smears by observing the photoluminescence of particles. PEG-SiDs were found nontoxic to S. aureus cells at concentrations of 100 mg\/mL, though a bacteriostatic effect on S. aureus biofilms was observed upon UV-A irradiation under conditions where light alone has no effect.","palabras_clave":"silicon dots|one-step synthesis|pegylation|aqueous suspension|photoluminescence|singlet molecular oxygen|S. aureus biofilms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6093,"idioma_id":4,"titulo":"Anisotropic Magnetoresistivity in Structured Elastomer Composites: modelling and experiments","resumen":"A constitutive model for the anisotropic magnetoresistivity in structured elastomer composites (SECs) is proposed. The SECs considered here are oriented pseudo-chains of conductive-magnetic inorganic materials inside an elastomer organic matrix. The pseudo-chains are formed by fillers which are simultaneously conductive and magnetic dispersed in the polymer before curing or solvent evaporation. The SEC is then prepared in the presence of a uniform magnetic field, referred to as Hcuring. This procedure generates the pseudo-chains, which are preferentially aligned in the direction of Hcuring. Electrical conduction is present in that direction only. The constitutive model for the magnetoresistance considers the magnetic pressure, Pmag, induced on the pseudo-chains by an external magnetic field, H, applied in the direction of the pseudo-chains. The relative changes in conductivity as a function of H are calculated by evaluating the relative increase of the electron tunnelling probability with Pmag, a magneto-elastic coupling which produces an increase of conductivity with magnetization. The model is used to adjust experimental results of magnetoresistance in a specific SEC where the polymer is polydimethylsiloxane, PDMS, and fillers are microparticles of magnetite?silver (referred to as Fe3O4[Ag]). Simulations of the expected response for other materials in both superparamagnetic and blocked magnetic states are presented, showing the influence of the Young´s modulus of the matrix and filler´s saturation magnetization.","palabras_clave":"composites|magnetoresistivity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6094,"idioma_id":4,"titulo":"The first quinoa (Chenopodium quinoa Willd) macrobotanical remains at Sierras del Norte (Central Argentina) and their implications in pre-Hispanic subsistence practices","resumen":"This paper presents a study of the first quinoa macrobotanical remains from the late pre-Hispanic site Quebrada Norte 7 (ca. 700 to 300 BP, Sierras of Norte, Córdoba, Argentina). The genus Chenopodium have been recorded since c. 7000 BP in several archaeological sites in Andean South America, from the Middle Holocene to the era of the Inca empire, suggesting that this plant has been used in multiple ways and has had economic and social importance continuing into the present time. Quinoa has been considered one of the primary subsistence resources available to societies in the sierras of Córdoba during the late Pre-Hispanic Period (800-1500 CE), but this inference was drawn from indirect data, such as Spanish documents and archaeological starch, grains, and pollen. The identification of Chenopodium quinoa was made via macrobotanical remains (achenes), examining quantitative and qualitative features. The presence of both quinoa and ajara, or quinoa negra, remains suggests that the Andean crop\/weed complex was part of subsistence system in Central Argentina.","palabras_clave":"ARCHAEOBOTANY|CENTRAL ARGENTINA|CHENOPODIUM QUINOA MACRO-REMAINS|CROP AND WEED|LATE PRE-HISPANIC PERIOD|QUEBRADA NORTE 7 SITE|SIERRAS OF C&Oacute;RDOBA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6095,"idioma_id":4,"titulo":"The Gaia-ESO Survey: Inhibited extra mixing in two giants of the open cluster Trumpler 20?","resumen":"Aims: We report the discovery of two Li-rich giants, with A(Li) ~1.50, in an analysis of a sample of 40 giants of the open clusterTrumpler 20 (with turnoff mass ~1.8 M&#8857;). The cluster wasobserved in the context of the Gaia-ESO Survey.  Methods: Theatmospheric parameters and Li abundances were derived usinghigh-resolution UVES spectra. The Li abundances were corrected fornonlocal thermodynamical equilibrium (non-LTE) effects.  Results:Only upper limits of the Li abundance could be determined for themajority of the sample. Two giants with detected Li turned out to be Lirich: star MG 340 has A(Li)non-LTE = 1.54 ± 0.21 dexand star MG 591 has A(Li)non-LTE = 1.60 ± 0.21 dex.Star MG 340 is on average ~0.30 dex more rich in Li than stars ofsimilar temperature, while for star MG 591 this difference is on average~0.80 dex. Carbon and nitrogen abundances indicate that all stars in thesample have completed the first dredge-up.  Conclusions: The Liabundances in this unique sample of 40 giants in one open clusterclearly show that extra mixing is the norm in this mass range. Giantswith Li abundances in agreement with the predictions of standard modelsare the exception. To explain the two Li-rich giants, we suggest thatall events of extra mixing have been inhibited. This includesrotation-induced mixing during the main sequence and the extra mixing atthe red giant branch luminosity bump. Such inhibition has been suggestedin the literature to occur because of fossil magnetic fields in redgiants that are descendants of main-sequence Ap-type stars.Based on observations collected at the European Organisation forAstronomical Research in the Southern Hemisphere under ESO programme188.B-3002 (The Gaia-ESO Public Spectroscopic Survey).","palabras_clave":"stars: abundances|stars: evolution|stars: late-type|open clusters and associations: individual: Trumpler 20"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6096,"idioma_id":5,"titulo":"Debates parlamentarios sobre la muerte digna en Argentina: los derechos de los pacientes terminales en la agenda legislativa (1996-2012)","resumen":"El artículo examina, a partir de fuentes secundarias, la producción legislativa sobre los derechos de los pacientes terminales en Argentina en dos momentos: los primeros intentos de regular los derechos en el final de la vida en el país (1996), y la reciente sanción de la ley de ?muerte digna? en el Congreso Nacional (2011-2012). El análisis comparativo permite observar variaciones en torno a las personas y situaciones incluidas en la legislación, la forma en que se conceptualiza la autonomía, y las intervenciones que se considera legítimo rechazar. El contexto político y la forma diferencial en que la ?muerte digna? ingresó en la agenda legislativa en cada periodo determinaron la construcción pública del problema, y con ello los alcances y el resultado de los debates.","palabras_clave":"Muerte digna|Medicalización|Eutanasia|Bioética"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6097,"idioma_id":4,"titulo":"The Gaia-ESO Survey: Probes of the inner disk abundance gradient","resumen":"Context. The nature of the metallicity gradient inside the solar circle (RGC < 8 kpc) is poorly understood, but studies of Cepheids and a small sample of open clusters suggest that it steepens in the inner disk.Aims. We investigate the metallicity gradient of the inner disk using a sample of inner disk open clusters that is three times larger than has previously been studied in the literature to better characterize the gradient in this part of the disk.Methods. We used the Gaia-ESO Survey (GES) [Fe\/H] values and stellar parameters for stars in 12 open clusters in the inner disk from GES-UVES data. Cluster mean [Fe\/H] values were determined based on a membership analysis for each cluster. Where necessary, distances and ages to clusters were determined via comparison to theoretical isochrones.Results. The GES open clusters exhibit a radial metallicity gradient of &#8722;0.10 ± 0.02 dex kpc-1, consistent with the gradient measured by other literature studies of field red giant stars and open clusters in the range RGC ~ 6&#8722;12 kpc. We also measure a trend of increasing [Fe\/H] with increasing cluster age, as has also been found in the literature.Conclusions. We find no evidence for a steepening of the inner disk metallicity gradient inside the solar circle as earlier studies indicated. The age-metallicity relation shown by the clusters is consistent with that predicted by chemical evolution models that include the effects of radial migration, but a more detailed comparison between cluster observations and models would be premature.","palabras_clave":"Galaxy: formation|Galaxy: disk|Galaxy: abundances|stars"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6098,"idioma_id":4,"titulo":"46,XX ovotesticular DSD associated with a SOX3 gene duplication in a SRY-negative boy","resumen":"Title: 46,XX ovotesticular DSD in the absence of SRY gene associated to SOX3 duplicationBackgroundOvotesticular DSD is a rare disorder defined by the presence of both ovarian and testicular tissue in the same individual. SRY is present in approximately 1\/3 of patients with 46,XX ovotesticular DSD. In SRY-negative ovotesticular DSD, the mechanism responsible for the presence of testicular tissue is not yet understood. Case presentation: A male patient was referred to us for hypospadias and bilateral cryptorchidism at 2.5 years of age. He had a trophic phallus (32 mm x 13 mm) with coronal hypospadias and hypoplastic scrotum. Right gonad was palpable in the inguinal region; no gonad was palpable on the left side. Basal AMH (216 pmol\/L) and hCG-stimulated testosterone (30 ng\/dl) were low, indicating that dysgenetic testicular tissue was present. Gonadotrophins were not elevated, with FSH predominance (LH <0.10 U\/L, FSH 0.73 U\/L). Karyotype was 46,XX. These results were suggestive of the presence of ovarian tissue. Diagnostic laparoscopy was performed, and the histopathological study confirmed the presence of bilateral ovotestes.Absence of SRY in peripheral leukocytes was documented by QF PCR analysis (Devyser Kit). A genome-wide copy number analysis, performed by single-nucleotide polymorphism using CytoSNP-850K microarray (Illumina), confirmed the absence of SRY and of Y chromosome sequences. Furthermore, a de novo duplication of 502,127 bp at Xq27.1 chromosomal region encompassing SOX3 gene was evidenced. Metaphase FISH analysis using a BAC probe hybridizing on both X homologues demonstrated a tandem duplication of this region.Conclusion and DiscussionThis is the first case of SRY-negative 46,XX Ovotesticular DSD in whom a genetic association (SOX3 duplication) is reported. These results are in line with evidence in mice indicating that, in the absence of SRY, gain-of-function of SOX3 induces testis differentiation in the XX bipotential gonad. SOX3, as a surrogate of SRY, would act synergistically with SF1 to upregulate SOX9 expression and stimulate testicular organogenesis","palabras_clave":"gonadal dysgenesis|gonadal determination|XX male|genomics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6099,"idioma_id":5,"titulo":"Los ungulados arcaicos de América del Sur: 'Condylarthra' y Litopterna.","resumen":"En el presente trabajo se describen sucintamente las principales problemáticas en torno al origen y evolución de los 'Condylarthra' del Paleógeno de América del Sur, así como los posibles vínculos en relación al origen de los denominados ungulados nativos. En particular, su evidente relación con los extintos Litopterna, formas sudamericanas y antárticas, que combinan una amplio espectro de morfologías dentales (i.e. bunodontes, bunolofodontes, lofoselenodontes), y por ende dietarias; con miembros caracterizados por dígitos impares, como en los Perissodactyla; pero con una amplia diversidad de formas que también recuerdan a ciertos Artiodactyla.","palabras_clave":"MAMMALIA|CONDYLARTHRA|LITOPTERNA|SUDAMÉRICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6100,"idioma_id":4,"titulo":"Iron biogeochemistry in Holocene palaeo and actual salt marshes in coastal areas of the Pampean Plain, Argentina","resumen":"In salt marshes, the hydrodynamics and theavailability of iron, organic matter and sulphate, influencethe formation and\/or dissolution of iron sulfides and ironoxyhydroxides. Therefore, they constitute key factorsaffecting the iron biogeochemical processes in these environments.The aim of this work is to evaluate the physicochemicaland mineralogical variations associated to ironbiogeochemistry in palaeo and actual salt marshes in thearea of influence of the Mar Chiquita coastal lagoon,Pampean Plain, Argentina. In soils of exhumed palaeomarshes, the iron contents are 56?95 lmol g-1, whereasthese contents decrease to 36?75 lmol g-1 in actual marshsoils. The presence of framboidal and poliframboidal pyritesassociated with gypsum, barite, calcite, halite and ironoxyhydroxides defines the conditions of the pedosedimentarysequences of the Holocene paleomarshes.Sequences of pyrite formation (sulfidization) and degradation(sulfuricization) were observed. These processeswere evidenced by a sequential extraction, reflecting thatthe largest proportion of iron is in the form of crystallineiron oxides (28?76 %) and lepidocrocite (6?16 %); whilethe proportion associated with ferrihydrite and pyrite is low(0?9 and 1?17 %, respectively). These facts could be partlyexplained by the complex redox processes characteristic ofthese environments, such as aeration generated by therhizosphere and intense bioturbation by invertebrates.These iron biomineralizations have been useful becausethey allow paleoenvironmental interpretations and characterizationof paleomarshes, and environmental inferencesrelated to the management of actual salt marshes.","palabras_clave":"Biomineralization| Framboidal and poliframboidal pyrites |Sequential extraction| Coastal wetlands"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6102,"idioma_id":4,"titulo":"Exploring the applicability of amorphous films of system In-Sb-Te as phase change materials","resumen":"Hereby, we present In-Sb-Te amorphous films grown by pulsed laser deposition technique using, as targets, crystalline ingots (with corresponding stoichiometry) prepared by traditional melt-quenching method. The explored nominal compositions were In50Sb15Te35, about a ternary compound formed in the quasi-binary InTe-SbTe system, metastable at room temperature, and two eutectics In8Sb8Te84 and In10Sb51Te39. Measurements of electrical sheet resistance evidenced that the amorphous films behave as electrical insulators at room temperature and present a giant jump in resistivity towards a conducting state on crystallization. Differential scanning calorimetry technique complemented the structural information obtained by X-ray diffraction and revealed temperatures of crystallization of the amorphous films as well as their melting points. Due to their temperature characteristics (crystallization temperature &#8776; 225 °C and melting temperature &#8776; 540 °C), In10Sb51Te39 film results very attractive from technological point of view. These characteristics could make this eutectic composition a good candidate for using in phase-change memory devices.","palabras_clave":"Chalcogenides| In-Sb-Te|Films|Phase change memory"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6103,"idioma_id":4,"titulo":"The 5S rDNA in two Abracris grasshoppers (Ommatolampidinae: Acrididae): molecular and chromosomal organization","resumen":"The 5S ribosomal DNA (rDNA) sequences are subject of dynamic evolution at chromosomal and molecular levels, evolving through concerted and\/or birth-and-death fashion. Among grasshoppers, the chromosomal location for this sequence was established for some species, but little molecular information was obtained to infer evolutionary patterns. Here, we integrated data from chromosomal and nucleotide sequence analysis for 5S rDNA in two Abracris species aiming to identify evolutionary dynamics. For both species, two arrays were identified, a larger sequence (named type-I) that consisted of the entire 5S rDNA gene plus NTS (non-transcribed spacer) and a smaller (named type-II) with truncated 5S rDNA gene plus short NTS that was considered a pseudogene. For type-I sequences, the gene corresponding region contained the internal control region and poly-T motif and the NTS presented partial transposable elements. Between the species, nucleotide differences for type-I were noticed, while type-II was identical, suggesting pseudogenization in a common ancestor. At chromosomal point to view, the type-II was placed in one bivalent, while type-I occurred in multiple copies in distinct chromosomes. In Abracris, the evolution of 5S rDNA was apparently influenced by the chromosomal distribution of clusters (single or multiple location), resulting in a mixed mechanism integrating concerted and birth-and-death evolution depending on the unit.","palabras_clave":"FISH|GENOME|MULTIGENE FAMILY| NON-TRANSCRIBED SPACER|REPETITIVE DNAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6104,"idioma_id":4,"titulo":"Difference between the vocalizations of two sister species of pigeons explained in dynamical terms","resumen":"&amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:Times;panose-1:2 0 5 0 0 0 0 0 0 0;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';mso-font-charset:78;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:1 0 16778247 0 131072 0;}@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:'';margin:0in;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:'Times New Roman';mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:'&amp;#65325;&amp;#65331; &amp;#26126;&amp;#26397;';mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:'Times New Roman';mso-bidi-theme-font:minor-bidi;mso-fareast-language:JA;}@page Section1{size:8.5in 11.0in;margin:1.0in 1.25in 1.0in 1.25in;mso-header-margin:.5in;mso-footer-margin:.5in;mso-paper-source:0;}div.Section1{page:Section1;}--&amp;gt;The study of animal behavior has shed light on the way a nervous system interacts with the peripheral effector organs in order to generate a specific motoro utput. In particular, vocal communication is a unique example where the nonlinear nature of the periphery can give rise to complex sounds even when driven by simple neural instructions. In this work we studied the case of twoclose-related bird species, Patagioenasmaculosa and Patagioenas picazuro,whose vocalizations differ only in the properties of their timbre. The temporal modulation of the fundamental frequency is similar in both cases, differing only in the existence of sidebands around the fundamental frequency in the case of the Patagioenas maculosa. We tested the hypothesis that the qualitative difference between these vocalizations lies in the nonlinear nature of the avian vocal organ.&nbsp; In particular, we propose that the roughness of maculosa´s vocalizationsis due to an asymmetry between the left and right vibratory membranes, whosenonlinear dynamics generate the sound. Inorder to test the hypothesis, we generated a biomechanical model for vocal production where we introduced an asymmetric parameter Q with which we can control the level of asymmetry between the vibratory membranes. Using this model we were able to generate synthetic vocalizations with the principal acoustic features of both species. In addition, we were able to confirm the anatomical prediction derived from this model, by making post-mortem inspectionof the syrinxes, showing that the species with tonal song (picazuro) has a more symmetrical pair of membranes compared to maculosa.","palabras_clave":"BIOMECHANICS|ASYMMETRY|VOCAL CONTROL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6105,"idioma_id":4,"titulo":"A revised and updated checklist of Monogononta rotifers from Argentina","resumen":"We provide here a checklist of species of Monogononta rotifers from lentic and lotic environments in Argentina, 25 years after the initial catalogue compiled by Susana B. José de Paggi. This new inventory now includes the reports on rotifers documented in 93 studies produced after 1990. The majority of the investigations were carried out in 3 of the 24 Argentine provinces. In addition, the presence of 13 species in samples from three water bodies within the Buenos Aires province are now cited here for the first time in Argentina. In this updated checklist, a total of 351 species are catalogued, the majority being representatives of the Lecanidae, Brachionidae, and Lepadellidae.","palabras_clave":"BIODIVERSITY|ROTIFERA|NEW RECORDS|INLAND WATERS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6106,"idioma_id":4,"titulo":"Dioctofimosis findings in canine renal clearances of a riparian area to La Plata river","resumen":"Dioctofimosis es una enfermedad parasitaria ocasionada por el gusano gigante del riñon, Dioctophyma renale, nematodo de ciclo biológico indirecto. Su hospedador natural es Mustela vison, sin embargo numerosos mamíferos domésticos, silvestres y el hombre pueden actuar como hospedadores definitivos, aunque en numerosas oportunidades albergan vermes en localizaciones ectópicas. Se estudiaron 692 caninos por ecografía, muestra de orina, cirugía, necropsia, e inspección clínica. Se diagnosticaron 244 (35,2%) casos de Dioctofimosis. No hubo diferencias estadísticamente significativas entre los diagnósticos realizados mediante análisis de orina y ecografías. Se presentan  los resultados obtenidos respecto de los métodos de diagnóstico utilizados y las localizaciones renales y extrarrenales de D. renale en los animales estudiados, 30,7% fueron diagnosticados mediante ecografía, 45,9% por análisis de orina y 17,6% por ambas técnicas. Hubo hallazgos de cirugía (2,4%), de necropsia (2,4%) y por eliminación espontánea de los parásitos (0,8%). Se observaron casos de dioctofimosis desde los 4 meses hasta los 15 años de edad. Las frecuencias de D. renale a lo largo del periodo de muestreo mostraron diferencias significativas. Se propone desarrollar técnicas diagnósticas indirectas para caninos, a efectos de diagnosticar estados precoces de la enfermedad, lo que contribuiría a la disminución de formas patentes, preservando así el ambiente y evitando la diseminación y expansión de esta parasitosis zoonótica.","palabras_clave":"Dioctophyma renale|prevalencia|asentamiento ribereño"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6107,"idioma_id":4,"titulo":"Reflection imaging of aseismic zones of the Nazca slab by global-phase seismic interferometry","resumen":"We have developed an application of passive seismic interferometry (SI) using P-wave coda of local earthquakes for the purpose of crustal-scale reflection imaging. We processed the reflection gathers retrieved from SI following a standard seismic processing in exploration seismology. We applied SI to the P-wave coda using crosscorrelation, crosscoherence, and multidimensional deconvolution (MDD) approaches for data recorded in the Malargüe region, Argentina. Comparing the results from the three approaches, we found that MDD based on the truncated singular-value decomposition scheme gave us substantially better structural imaging. Although our results provided higher resolution images of the subsurface, they showed less clear images for the Moho in comparison with previous seismic images in the region obtained by the receiver function and global-phase SI. Above the Moho, we interpreted a deep thrust fault and the possible melting zones, which were previously indicated by active-seismic and magnetotelluric methods in this region, respectively. The method we developed could be an alternative option not only for crustal-scale imaging, e.g., in enhanced geothermal systems, but also for lithospheric-scale as well as basin-scale imaging, depending on the availability of local earthquakes and the frequency bandwidth of their P-wave coda.","palabras_clave":"acoustic|crustal structure|earthquake|interferometry|geothermal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6109,"idioma_id":5,"titulo":"Psychometric properties of the Attitudes Toward Gay men scale in Argentinian context: The influence of sex, authoritarianism, and social dominance orientation","resumen":"Even though prejudice toward male homosexuality is one of the main reasons for discrimination in Argentina, there is no valid measure to assess it. The aim of this study was to analyze the psychometric properties of the Attitudes Toward Gay Men Scale (ATG) and to examine the influence of sex, right wing authoritarianism, and social dominance orientation on anti-gay attitudes. Data were collected with a convenience sample of 436 undergraduate students from University of Buenos Aires. Analysis of the data showed adequate psychometric properties for the ATG Scale and the moderating effect of sex, right wing authoritarianism and social dominance orientation on anti-gay attitudes. Implications of these findings were discussed.","palabras_clave":"Homosexuality|Prejudice|Authoritarianism|Dominance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6110,"idioma_id":5,"titulo":"El estudio psicológico del prejuicio: Aportes del autoritarismo y la dominancia social","resumen":"El prejuicio definido como una antipatía basada en una generalización inflexible y errónea, dirigida hacia un grupo como totalidad o hacia un individuo por ser miembro del mismo, ha sido considerado como un fenómeno generalizado. En este sentido, se ha demostrado que individuos que suelen presentar elevados niveles de prejuicio hacia un grupo social en particular, generalmente también manifiestan altos niveles hacia otros. Uno de los principales instrumentos que se han desarrollado para la evaluación del prejuicio generalizado ha sido el termómetro de calificaciones afectivas hacia grupos sociales, el cual presenta una estructura multifactorial compuesta por diferentes prejuicios hacia grupos considerados como peligrosos, derogados y disidentes. Diferentes estudios indican que dos rasgos de personalidad, el autoritarismo del ala de derechas y la orientación a la dominancia social, predicen diferentes formas de prejuicio. Mientras que el autoritarismo del ala de derechas expresa una motivación por mantener el orden y la seguridad grupal, siendo su correlato el prejuicio hacia grupos percibidos como peligrosos, la dominancia social refleja una motivación por el dominio y la superioridad, cuyo correlato es el prejuicio hacia grupos percibidos como derogados. Además, producto de las motivaciones subyacentes en ambas variables, predicen conjuntamente el prejuicio hacia grupos disidentes, que por un lado representan una competencia, desafiando el sostenimiento de la desigualdad social, mientras que por otro, amenazan el orden, la estabilidad y la cohesión social. Este estudio analiza las diferentes perspectivas para el estudio del fenómeno del prejuicio generalizado.","palabras_clave":"PREJUICIO|ESTEREOTIPOS|DISCRIMINACIÓN|AUTORITARISMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6111,"idioma_id":4,"titulo":"The Constant-Sound-Speed parameterization for NJL models of quark matter in hybrid stars","resumen":"The discovery of pulsars as heavy as 2 solar masses has led  astrophysicists to rethink the corecompositions of neutron stars, ruling out many models for the nuclear equations of state (EoS).We explore the hybrid stars that occur when hadronic matter is treated in a relativistic mean-field approximation and quark matter is modeled by three-flavor local and non-local Nambu&#8722;Jona-Lasinio (NJL) models with repulsive vector interactions. The NJL models typically yield equationsof state that feature a first-order transition to quark matter. Assuming that the quark-hadronsurface tension is high enough to disfavour mixed phases, and restricting to EoSs that allow startsto reach 2 solar masses, we find that the appearance of the quark matter core either destabilizesthe star immediately (this is typical for non-local NJL models) or leads to a very short hybrid starbranch in the mass-radius relation (this is typical for local NJL models). Using the Constant-Sound-Speed parametrization we can see that the reason for the near-absence of hybrid stars is that thetransition pressure is fairly high and the transition is strongly first-order.","palabras_clave":"Hybrid stars|NJL models|constant-sound-speed parametrization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6112,"idioma_id":4,"titulo":"First record of Hypostomus boulengeri (Siluriformes: Loricariidae) from Bermejo River basin","resumen":"Hypostomus boulengeri (Eigenmann and Kennedy, 1903) is a poorly known species recorded from the Paraguay and Paraná River basins. In this work we report the occurrence of this species in the Bermejo River basin for the first time, representing a distribution range extension of more than 600 km.","palabras_clave":"fishes|biogeography|Salta|Río de la Plata basin|Hypostominae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6113,"idioma_id":5,"titulo":"Inseminación artificial en cerdas: ¿es aplicable la post-cervical en nulíparas?","resumen":"La carne porcina es la más consumida en elmundo, representando un 42,79% del consumototal (184,6 millones de toneladas) según datosde la Organización de las Naciones Unidas para laAlimentación y la Agricultura. China, UniónEuropea y Estados Unidos, son los principales productoresy consumidores de carne de cerdo a nivelmundial (3). Aunque la producción porcina en laArgentina aún se encuentra en desarrollo, su consumose cuadruplicó en los últimos diez años (17) yes una actividad con enorme potencial de crecimientoya que se dispone de los recursos necesarios(suelo, agua dulce, clima benigno y gran producciónde granos, base nutricional del cerdo) (13).La inseminación artificial (IA) permite mejorar lagenética de una piara en un 50% en una generación.Además optimiza la productividad delmacho, preserva la higiene y sanidad de los reproductoresy su descendencia porque evita el contactodirecto entre animales. Otra gran ventajade la IA es que posibilita la selección de animalesdestacados por su genética, facilita la organizaciónzootécnica a escala industrial y representauna disminución de los costos de producción","palabras_clave":"Inseminación artificial|Porcinos|Inseminación post-cervical|Nulíparas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6114,"idioma_id":5,"titulo":"Expresión material de la interacción entre indígenas y criollos en un sitio funerario del siglo XIX de Norpatagonia (Sitio Chimpay, Río Negro)","resumen":"En este trabajo se presentan los resultados del análisis de un contexto funerario de fines del siglo XIX, procedente de la localidad de Chimpay (noreste de Patagonia, Argentina). En el sitio se hallaron restos humanos de dos individuos adultos, un hombre y una mujer, con un abundante y diverso acompañamiento funerario que incluía prendas, accesorios y otros materiales dejados junto a los cuerpos como ofrenda. El foco principal del trabajo está puesto en el estudio de estos materiales asociados a los cuerpos, algunos típicamente indígenas y otros característicos de la sociedad hispano-criolla. Sobre la base de esto se concluye que las personas enterradas tenían una posición social y\/o política de jerarquía. El hombre posiblemente se desempeñó como interlocutor indígena frente a las autoridades del gobierno nacional y, para ello, habría recibido algún cargo militar.","palabras_clave":"Arueología|Siglo XIX|Entierro doble"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6115,"idioma_id":4,"titulo":"Feature-tailored spectroscopic analysis of the supernova remnant Puppis A in X-rays","resumen":"We introduce a distinct method to perform spatially-resolved spectral analysis of astronomical sources with highly structured X-ray emission. Themethod measures the surface brightness of neighbouring pixels to adaptivelysize and shape each region, thus the spectra from the bright and faintfilamentary structures evident in the broadband images can be extracted. As atest case, we present the spectral analysis of the complete X-ray emittingplasma in the supernova remnant Puppis A observed with XMM-Newton and Chandra.Given the angular size of Puppis A, many pointings with different observationalconfigurations have to be combined, presenting a challenge to any method ofspatially-resolved spectroscopy. From the fit of a plane-parallel shockedplasma model we find that temperature, absorption column, ionization timescale, emission measure and elemental abundances of O, Ne, Mg, Si, S and Fe,are smoothly distributed in the remnant. Some regions with overabundances ofO-Ne-Mg, previously characterized as ejecta material, were automaticallyselected by our method, proving the excellent response of the technique. Thismethod is an advantageous tool for the exploitation of archival X-ray data.","palabras_clave":"ISM: SNR|X-RAY: ISM|PUPPIS A"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6116,"idioma_id":4,"titulo":"The environment of the gamma-ray emitting SNR G338.3-0.0: a hadronic interpretation for HESS J1640-465","resumen":"The supernova remnant (SNR) G338.3-0.0 spatially correlates with HESSJ1640-465, which is considered the most luminous gamma-ray sourceassociated with a SNR in our Galaxy. The X-ray pulsar PSR J1640-4631 hasbeen recently discovered within the SNR shell, which could favor aleptonic origin for the detected very-high energy (VHE) emission. Inspite of this, the origin of the VHE radiation from HESS J1640-465 hasnot been unambiguously clarified so far. Indeed, a hadronic explanationcannot be ruled out by current observations. On the basis of atomic (HI)and molecular (12^CO) archival data, we determine, for the first time,the total ambient density of protons in the region of theG338.3-0.0\/HESS J1640-465 system, a critical parameter for understandingthe emission mechanisms at very high energies. The value obtained is inthe 100-130 cm^-3 range. Besides this, we developed a new hadronic modelto describe the spectral energy distribution (SED) of the HESS J1640-465source, which includes the latest total gamma-ray cross-section forproton-proton collisions available in the literature. By using theassessed ambient proton density, we found that the total energy inaccelerated protons required to fit the data is 5.4^{+4.7}_{-2.3} x 10^49 erg and 1.6^{+1.4}_{-0.7} x 10^50 erg for a source distance of 8.5and 13 kpc, respectively. The case where the source distance is 8.5 kpcagrees with the typical scenario in which the energy released is on theorder of 10^51 erg and ~10% of that energy is transferred to theaccelerated protons, whereas the case corresponding to a source distanceof 13 kpc requires either a higher value of the energy released in theexplosion or a larger energy fraction to accelerate protons.","palabras_clave":"G338.3-0.0|HESS J1640-465|SNR|MIE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6117,"idioma_id":5,"titulo":"MECANISMOS DE PROTECCION MIOCARDICA A DISTANCIA","resumen":"Objetivos: Ha sido propuesto que el precondicionamiento isquémico remoto (PCr) reduce el tamaño de infarto a través de la activación de una vía neuronal parasimpática. Sin embargo los mecanismos intracelulares responsables de esta protección no son completamente conocidos. El objetivo fue describir algunas de las señales intracelulares activadas a nivel cardíaco por el PCr, antes de la isquemia miocárdica y que participan en la protección.Métodos y resultados: Corazones aislados de ratas fueron sometidos a 30 minutos de isquemia global, seguido de 120 minutos de reperfusión (I\/R). En un segundo grupo antes del aislamiento del corazón, se realizó un protocolo de PCr (3 ciclos de isquemia\/reperfusión en la arteria femoral izquierda), una vez finalizado  se repitió el protocolo del grupo I\/R. Adicionalmente se estudiaron cuatro grupos experimentales, donde antes del PCr se realizó una vagotomía cervical bilateral (SV) o se administró atropina (bloqueante de los receptores muscarínicos), L-NAME (inhibidor de la síntesis de NO) y 5-HD (bloqueante de los canales mK+ATP), respectivamente. Se midió el tamaño de infarto y la fosforilación de la eNOS  en los grupos I\/R, PCr, y SV. Por otro lado se midió la producción mitocondrial de  H2O2.  El PCr redujo significativamente el tamaño de infarto y este efecto fue abolido por la SV y con los tratamientos con atropina, L-NAME y 5-HD. Además, el PCr incrementó la fosforilación de la eNOS, y este efecto fue abolido por la SV. Finalmente el PCr produjo un aumento de la producción de H2O2 mitocondrial, hecho que también fue abolido con la SV.Conclusions: El PCr activa una via muscarínica vagal, que involucra la fosforilación de la eNOS, la apertura de los canales mK+ATP  de la mitocondria y un aumento de la producción de H2O2  mitocondrial.  Objetivos: Ha sido propuesto que el precondicionamiento isquémico remoto (PCr) reduce el tamaño de infarto a través de la activación de una vía neuronal parasimpática. Sin embargo los mecanismos intracelulares responsables de esta protección no son completamente conocidos. El objetivo fue describir algunas de las señales intracelulares activadas a nivel cardíaco por el PCr, antes de la isquemia miocárdica y que participan en la protección.Métodos y resultados: Corazones aislados de ratas fueron sometidos a 30 minutos de isquemia global, seguido de 120 minutos de reperfusión (I\/R). En un segundo grupo antes del aislamiento del corazón, se realizó un protocolo de PCr (3 ciclos de isquemia\/reperfusión en la arteria femoral izquierda), una vez finalizado  se repitió el protocolo del grupo I\/R. Adicionalmente se estudiaron cuatro grupos experimentales, donde antes del PCr se realizó una vagotomía cervical bilateral (SV) o se administró atropina (bloqueante de los receptores muscarínicos), L-NAME (inhibidor de la síntesis de NO) y 5-HD (bloqueante de los canales mK+ATP), respectivamente. Se midió el tamaño de infarto y la fosforilación de la eNOS  en los grupos I\/R, PCr, y SV. Por otro lado se midió la producción mitocondrial de  H2O2.  El PCr redujo significativamente el tamaño de infarto y este efecto fue abolido por la SV y con los tratamientos con atropina, L-NAME y 5-HD. Además, el PCr incrementó la fosforilación de la eNOS, y este efecto fue abolido por la SV. Finalmente el PCr produjo un aumento de la producción de H2O2 mitocondrial, hecho que también fue abolido con la SV.Conclusions: El PCr activa una via muscarínica vagal, que involucra la fosforilación de la eNOS, la apertura de los canales mK+ATP  de la mitocondria y un aumento de la producción de H2O2  mitocondrial.  ","palabras_clave":"precondicionamiento"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6118,"idioma_id":4,"titulo":"Myocardial triggers involved in remote ischemic preconditioning activation","resumen":"ABSTRACTAims: It has been proposed that remote ischemic preconditioning (rIPC) activates a parasympathetic neural pathway. However, the myocardial rIPC intracellular mechanism remains unclear. Here we characterized some of the intracellular signals participating as rIPC triggers. Methods and Results: Isolated rat hearts were subjected to 30 minutes of global ischemia and 120 minutes of reperfusion (Non-rIPC). In a second group, before the isolation of the heart, a rIPC protocol (3 cycles of hindlimb ischemia\/reperfusion) was performed. The infarct size was measured with tetrazolium staining. Akt and eNOS expression\/phosphorylation, and mitochondrial H2O2 production were evaluated at the end of rIPC protocol, before myocardial ischemia\/reperfusion. rIPC significantly decreased the infarct size and induced Akt and eNOS phosphorylation. The protective effect on infarct size was abolished by cervical vagal section (CVS), L-NAME (NO synthesis inhibitor) and 5-HD (mK+ATP channels blocker). Mitochondrial production of H2O2 was increased by rIPC while it was abolished by CVS, L-NAME and 5-HD. Conclusions: rIPC activates a parasympathetic vagal pathway and a mechanism involving the Akt and eNOS phosphorylation, the opening of mK+ATP, and the release of H2O2 by the mitochondria. These entire phenomena occur prior to the myocardial ischemia, which could act as triggers of rIPC.","palabras_clave":"PRECONDITIONING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6119,"idioma_id":4,"titulo":"Characterization of routes of metastasis from Absorbent Markov Chains","resumen":"Metastasis is a complex and multi-step stochastic process. The study of the probabilities of generating a tumor from a primary site in another organs is the aim of this work. Based on statistics of National Institute of Cancer of Argentina (INC), a characterization of the routes of metastasis for the principal organs is presented by using Absorbing Markov chains. The metastasis propagation fromdifferent primary sites towards secondary and tertiary sites is also shown, emphasizing the relation and analysis about absorbing states.","palabras_clave":"MARKOV CHAINS|METASTASIS|CANCER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6120,"idioma_id":4,"titulo":"?\tProtective Effect of Fosfomycin on Deoxynivalenol ? Treated Cell Cultures","resumen":"Protective Effect of Fosfomycin on Deoxynivalenol ? Treated Cell Cultures","palabras_clave":"Fosfomycin|Deoxynivalenol|Cell cultures|Hep-2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6121,"idioma_id":4,"titulo":"Low-pressure equilibrium binary argon\/methane gas mixture adsorption on exfoliated graphite: Experiments and simulations","resumen":"Adsorption equilibrium measurements of pure methane, pure argon, and binary mixtures over exfoliated graphite were carried for different initial compositions, temperatures, and total pressures in the range of 0.1?1.5 Torr using the volumetric static method. Diagrams for gas and adsorbed phase compositions were constructed for the conditions explored, and isosteric heats of adsorption were calculated. Experimental results were compared with predictions obtained with Monte Carlo simulations and using the Ideal Adsorbed Solution Theory (IAST).","palabras_clave":"Adsorption|Argon\/Methane Mixtures|Numerical Simulations|Separations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6122,"idioma_id":4,"titulo":"A turning point in argentine politics: demands for change and territorial cleavages in the 2015 presidential election","resumen":"The 2015 national, subnational, executive and legislative elections marked a turning point for Argentina?s democratic history. For the first time, a non- Peronist centre-right coalition won the presidency. These elections also inaugurated the first non-Peronist governor of the Province of Buenos Aires in almost 30 years. This article tries to make sense of these elections, which represent a significant shift in the country?s political direction. We argue that the incumbent left-party (Peronist Frente para la Victoria) faced a combination of electoral fatigue and mounting economic challenges. Sluggish economic growth and unabated inflation created an anti-incumbent demand for change, alienated the middle class and deepened territorial cleavages between the agricultural central region and the peripheral provinces. Against this backdrop, the combination of a unified opposition and a fragmented Peronism led to Mauricio Macri?s victory. We also explore the challenges that the newly elected president faces, including minority in Congress and among governors.","palabras_clave":"ARGENTINE ELECTIONS 2015|MAURICIO MACRI|TERRITORIAL CLEAVAGES|PRESIDENTIAL TURNOVER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6123,"idioma_id":4,"titulo":"LIOLAEMUS CHACOENSIS (Chaco´s Lizard). AVIAN PREDATION.","resumen":"Liolaemus chacoensis is a small lizard (up to 55 mm SVL) occurring in the Chaco EcoregioN. Its distribution includes the countries of Argentina, Paraguay. Reptiles del noroeste, nordeste y este de la Argentina. In San Juan, it inhabits the Occidental Chaco Ecoregion. It lives in the dense vegetation of the Chaco forest. Here we report observed predation on L. chacoensis by the Spot-winged Falconet (Spiziapteryx circumcincta). At 1755 hours on the 20th of September 2014, during a field work in La Majadita, Valle Fértil, San Juan Province, Argentina (30.71306°S, 67.49556°W, datum WGS84; 976 m elev.), we observed a spot-winged falconet perched on a Prosopis chilensis tree, calling for food. Few minutes later, another falcon arrived carrying on its beak an adult of L. chacoensis by the neck. Inmediately afterwards, the spot-winged falconet ate its prey and flew away, and the other falconet followed. This is the first record of predation on L. chacoensis by S. circumcincta. It is well-known that S. circumcincta feeds on lizards but here we first documented one of the species that make up its diet.","palabras_clave":"LIOLAEMUS CHACOENSIS|AVIAN PREDATION|CHACO|SPIZIAPTERYXS CIRCUMCINCTA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6124,"idioma_id":5,"titulo":"Primer registro de depredación de murciélagos por el halconcito colorado (Falco sparverius) en la ciudad de Mendoza, provincia de Mendoza, Argentina.","resumen":"La dieta del Halconcito Colorado (Falco sparverius) ha sido muy estudiada en Argentina. Se alimenta principalmente de insectos, pero también de vertebrados como anfibios, reptiles y  pequeñas aves y mamíferos (Beltzer 1990, De la Peña 2002, Sarasola et al. 2003, Castro Cabral et al. 2006, Zilio 2006, Santillán et al. 2009). La presencia de quirópteros en la dieta del Halconcito Colorado, es inusual (Smallwood & Bird 2002) y, en Sudamérica ha sido documentada en Brasil (Aguiar et al. 2012), Chile (Rodríguez-San Pedro & Allendes 2015) y la costa de la Patagonia Argentina (Pagnoni 2013).El 11 de diciembre de 2015, a las 17:49 h, en el balcón de un departamento en una zona urbana de la  ciudad de Mendoza, provincia de Mendoza, Argentina (32°53´37?S, 68°50´30?O) observamos un macho de Halconcito colorado (Falco sparverius) con un murciélago en sus garras, cuya especie no pudimos identificar (Fig. 1). Esta observación amplía el número de registros de consumo de quirópteros por el Halconcito colorado. Aunque la predación de murciélagos por aves rapaces diurnas parece ser subestimada e insuficientemente estudiada, es probable que sea más frecuente y mayor distribuida que lo que se creía (Mikula et al. 2016). Particularmente en Argentina la dieta del Halconcito colorado (F. sparverius) ha sido bien estudiada en áreas naturales (Beltzer 1990, De la Peña 2002, Sarasola et al. 2003, Santillán et al. 2009). Sin embargo, sólo existe un registro previo de depredación de quirópteros por esta especie en el país (Pagnoni 2013) en un área urbana, similar a lo reportado en este artículo. Este comportamiento que ha sido considerado como inusual en artículos que evalúan la dieta en áreas naturales podría resultar  más frecuente en áreas urbanas. Consideramos necesario realizar más investigaciones en estas áreas para poder corroborar las diferencias mencionadas.","palabras_clave":"PREDACION |MURCIELAGOS|RAPACES|MENDOZA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6125,"idioma_id":4,"titulo":"Feeding behavior and social interactions of  the Argentine ant Linepithema humile change with sucrose concentration","resumen":"Liquid sugar baits are well accepted by the Argentine ant Linepithema humile andare suitable for the chemical control of this invasive species. We evaluated how sugar concentrations affect the foraging behavior of L. humile individuals. We quantified feeding variables for individual foragers (ingested load, feeding time and solution intake rate) when feeding on sucrose solutions of different concentrations, as well as post-feeding interactions with nestmates. Solutions of intermediate sucrose concentrations (10?30%) were the most consumed and had the highest intake rates, whereas solutions of high sucrose concentrations (60 and 70%) resulted in extended feeding times, low intake rates and ants having smaller crop loads. In terms of postfeeding interactions, individuals fed solutions of intermediate sucrose concentrations (20%) had the highest probability of conducting trophallaxis and the smallest latency to drop exposure (i.e. lowest time delay). Trophallaxis duration increased with increasing sucrose concentrations. Behavioral motor displays, including contacts with head jerking and walking with a gaster waggle, were lowest for individuals that ingested the more dilute sucrose solution (5%). These behaviors have been previously suggested to act as a communication channel for the activation and\/or recruitment of nestmates. We show here that sucrose concentration affects feeding dynamics and modulates decision making related to individual behavior and social interactions of foragers. Our results indicate that intermediate sucrose concentrations (ca. 20%), appear to be most appropriate for toxic baits because they promote rapid foraging cycles, a high crop load per individual, and a high degree of stimulation for recruitment.","palabras_clave":"ARGENTINE ANTS|TROPHALLAXIS|FEEDING BEHAVIOR|FORAGING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6126,"idioma_id":4,"titulo":"Biodecoloration of Reactive black 5 by the methylotrophic yeast Candida boidinii MM 4035","resumen":"Martorell M.M., Pajot H.F.& L.I.C. de Figueroa (2016). Candida sp MM-4035, a methanol-assimilating yeast with dye-decolorizing ability. Dye removal optimization, phenotypical and genetic characterization. International Biodeterioration & Biodegradation, ISSN 0964-http:\/\/dx.doi.org\/10.1016\/j.jes.2016.01.033","palabras_clave":"Candida sp |Methanol-assimilating yeast|Dye removal|Phenotypical and genetic characterization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6127,"idioma_id":4,"titulo":"Hepatitis B virus infection in blood donors in Argentina: prevalence of infection, genotype distribution and frequency of occult HBV infection","resumen":"This study describes the prevalence of HBV infection based on detection of HBsAg and HBV-DNA by NAT in 70,102 blood donors in Argentina (Córdoba province) and shows the viral genotype distribution and frequency of occult HBV infection (OBI) in this population. Forty-two donors were confirmed positive for HBV infection (0.06 %), and four had OBI. Genotype F was the most prevalent (71.4 %), followed by A (14.3 %), C (7.1 %) and D (7.1 %). This is the first report of the prevalence of confirmed HBV infection and the high frequency of occult HBV infection in a blood bank in Argentina.","palabras_clave":"HBV|OCCULT HBV infection|GENOTYPE|NAT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6128,"idioma_id":5,"titulo":"HABILIDADES FONOLÓGICAS, PRECISIÓN LECTORA Y VELOCIDAD EN PACIENTES CON DISLEXIA","resumen":"La conciencia fonolo&#769;gica es un tema central en las investigaciones sobre lectura y esta&#769; relacionada con las di cultades lectoras. Sin embargo, investigaciones realizadas en distintos sistemas ortogra&#769; cos sen&#771;alan que la velocidad de procesamiento tambie&#769;n puede ser una causa del de&#769; cit lector. En este marco, el propo&#769;sito de este trabajo fue comparar el rendimiento de cinco pacientes disle&#769;xicos con el de tres grupos controles de nin&#771;os de 1er grado, 3er grado y 5to grado de nivel primario sin di cultades en el aprendizaje de la lectura. Los participantes normolectores y los pacientes fueron evaluados con tres pruebas que evalu&#769;an conciencia fonolo&#769;gica disen&#771;ada ad hoc y una tarea de lectura de palabras y no palabras estandarizada. Se registraron tanto las respuestas correctas como el tiempo requerido para la resolucio&#769;n de las tareas. En base a los datos, se pudieron identi car distintos per les de rendimiento en los pacientes disle&#769;xicos segu&#769;n la cantidad de aciertos y la velocidad para resolver las tareas.*Revista Indexada en: INFOCyT, PEPSIC, CLASE, E-Revistas, DIALNET, EBSCO, Latindex catálogo, FEAP, DOAJ, Redalyc y Thomson Reuters.","palabras_clave":"conciencia fonológica|lectura|dislexia|velocidad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6129,"idioma_id":4,"titulo":"Avalanche criticalities and elastic and calorimetric anomalies of the transition from cubic Cu-Al-Ni to a mixture of 18R and 2H structures","resumen":"We studied the two-step martensitic transition of a Cu-Al-Ni shape-memory alloy by calorimetry, acoustic emission (AE), and resonant ultrasound spectroscopy (RUS) measurements. The transition occurs under cooling from the cubic (&#946;, Fm3m) parent phase near 242 K to a mixture of orthorhombic 2H and monoclinic 18R phases. Heating leads first to the back transformation of small 18R domains to &#946; and\/or 2H near 255 K, and then to the transformation 2H to &#946; near 280 K. The total transformation enthalpy is HT = 328 ± 10 J\/mol and is observed as one large latent heat peak under cooling. The back-transformation entropy under heating breaks down into a large component 18R to &#946; at 255 K and a smaller, smeared component of the transformation 2H to &#946; near 280 K. The proportions inside the phase mixture depend on the thermal history of the sample. The elastic response of the sample is dominated by large elastic softening during cooling. The weakening of the elastic shear modulus shows a peak at 242 K, which is typical for the formation of complex microstructures. Cooling the sample further leads to additional changes of the microstructure and domain wall freezing, which is seen by gradual elastic hardening and increasing damping of the RUS signal. Heating from 220 K to room temperature leads to elastic anomalies due to the initial transformation, which is now shifted to high temperatures. The transition is smeared over a wider temperature interval and shows strong elastic damping. The shear modulus of the cubic phase is recovered at 280 K. The phase transformation leads to avalanches, which were recorded by AE and by time-resolved calorimetry. The cooling transition shows very extended avalanche signals in calorimetry with power-law distributions. Cooling and heating runs show AE signals over a large temperature interval above 260 K. Splitting the transformation into two martensite phases leads to power-law exponents &#949; &#8764; 2 (&#946; &#8596; 18R) and &#949; &#8764; 1.5 (&#946; &#8596;2H) while the phase mixture shows an effective AE exponent of 1.7.","palabras_clave":"calorimetry|acoustic emission|resonant ultrasound spectroscopy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6130,"idioma_id":4,"titulo":"Histomorphometry of the tibia and mandible of healthy female Wistar rats at different stages of growth","resumen":"Abstract: Female Wistar rats are frequently used in experimental models to study hormone and bone pathologies and treatments. Most experimental studies involving histomorphometric evaluation assessed long bones, and few reports also studied mandibular bone. The aim of this work was to clarify and distinguish the age-related histomorphometric changes that occur in the tibia (subchondral bone) and in the mandible (interradicular bone), and thus obtain reference histomorphometric data of healthy female Wistar rats at different growth stages. Three groups of 8 healthy female Wistar rats were euthanized at 6 (GI), 10 (GII), and 14 (GIII) weeks. The tibiae and mandible were resected and histologically processed to obtain H&E stained sections of the tibia and the lower first molar to analyze the following histomorphometric parameters: Bone volume (BV\/TV)(%), trabecular width (Tb.Wi)(µm), trabecular number (Th.N)(1\/mm), growth cartilage width (GPC.Wi)(µm), hypertrophic cartilage width (HpZ. Wi)(µm) and number of osteoclasts per area (N.Oc\/mm2) in the tibiae, and bone volume (BV\/ TV)(%) and number of osteoclasts per area N.Oc\/mm2 in the interradicular bone of the first lower molar. A significant decrease in subchondral bone volume as a result of a decrease in trabecular number and growth cartilage width was observed in 14-week old rats. Conversely, interradicular bone volume was found to increase with age. The results highlight the importance of analyzing both types of bone to better understand the response of two different trabecular bones, contributing in turn to decision making regarding treatment strategies and disease management.","palabras_clave":"BONE HISTOMORPHOMETRY|HEALTHY WISTAR RATAS|LONG BONES|MANDIBULAR BONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6131,"idioma_id":5,"titulo":"1976-2016 Conmemoraciones","resumen":"El articulo constituye la introducción al dossier compilado junto a la Dra. Melendo en ocasión del 40. aniversario del inicio de la última dictadura. Allí se discuten cuestiones tales como la especificidad\/ privilegio del arte para la representación del pasado, la distancia histórica, el papel de la dimensión afectiva, y la relación constitutiva entre el pasado disrruptivo y el presente","palabras_clave":"Arte|Hisotoria|Dictadura|Representacion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6132,"idioma_id":4,"titulo":"Estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish.","resumen":"Abstract Ghrelin, and nesfatin-1 (encoded bynucleobindin2\/nucb2) are twometabolic peptides with multiple biological effects in vertebrates. While sexsteroids are known to regulate endogenous ghrelin and NUCB2 in mammals, suchactions by steroids in fish remain unknown. This study aimed to determinewhether estradiol (E2) and testosterone (T) affects the expression ofpreproghrelin, ghrelin\/growth hormone secretagogue receptor (GHS-R), ghrelinO-acyl transferase (GOAT) and NUCB2 in goldfish (Carassius auratus). First, a dose-response assay was performed inwhich fish were intraperitoneally (ip) implanted with pellets containing 25, 50or 100 µg\/g body weight (BW) of E2 or T. It was found that sex steroids (100µg\/g BW) administered for 2.5 days achieved the highest E2 or T in circulation.In a second experiment, fish were ip implanted with pellets containing 100 µg\/gBW of E2, T or without hormone (control). RT-qPCR analyses at 2.5 dayspost-administration show that gut preproghrelin and GOAT expression was upregulatedby both E2 and T treatments, while the same effect was observed for GHS-R onlyin the pituitary. Both treatments also reduced hypothalamic preproghrelin mRNAexpression. NUCB2 expression was increased in the forebrain of T treated groupand reduced in the gut and pituitary under both treatments. These results showfor the first time a modulation of preproghrelinand nucb2\/nesfatin-1 by sex steroidsin fish. The interaction between sex steroids and genes implicated in bothmetabolism and reproduction might help meeting the reproduction dependentenergy demands in fish.","palabras_clave":"Ghrelin|Nesfatin-1|Food intake| Reproduction|Sex steroid hormones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6133,"idioma_id":4,"titulo":"Nesfatin-1-Like Peptide Encoded in Nucleobindin-1 in Goldfish is a Novel Anorexigen Modulated by Sex Steroids, Macronutrients and Daily Rhythm.","resumen":"Nesfatin-1 is an 82 amino acid anorexigen encoded in a secreted precursor nucleobindin-2 (NUCB2). NUCB2 was named so due to its high sequence similarity with nucleobindin-1 (NUCB1). It was recently reported that NUCB1 encodes an insulinotropic nesfatin-1-like peptide (NLP) in mice. Here, we aimed to characterize NLP in fish. RT- qPCR showed NUCB1 expression in both central and peripheral tissues. Western blot analysis and\/or fluorescence immunohistochemistry determined NUCB1\/NLP in the brain, pituitary, testis, ovary and gut of goldfish. NUCB1 mRNA expression in goldfish pituitary and gut displayed a daily rhythmic pattern of expression. Pituitary NUCB1 mRNA expression was downregulated by estradiol, while testosterone upregulated its expression in female goldfish brain. High carbohydrate and fat suppressed NUCB1 mRNA expression in the brain and gut. Intraperitoneal injection of synthetic rat NLP and goldfish NLP at 10 and 100 ng\/g body weight doses caused potent inhibition of food intake in goldfish. NLP injection also downregulated the expression of mRNAs encoding orexigens, preproghrelin and orexin-A, and upregulated anorexigen cocaine and amphetamine regulated transcript mRNA in goldfish brain. Collectively, these results provide the first set of results supporting the anorectic action of NLP, and the regulation of tissue specific expression of goldfish NUCB1.","palabras_clave":"Nesfatin-1 like peptide|Goldfish|Sex steroid|Macronutrients"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6134,"idioma_id":4,"titulo":"Enzymatic deglycosylation of flavonoids in deep eutectic  solvents-aqueous mixtures: paving the way for sustainable flavonoid  chemistry","resumen":"The low solubility of glycosylated flavonoids represents a hurdle to conduct efficient enzymatic deglycosylations in aqueous media. To overcome this drawback, environmentally-unfriendly dimethylsulfoxide (DMSO) is typically used as co-solvent. Using a specific diglycosidase from Acremonium sp. DSM24697 for the deglycosylation of the rutinosylated flavonoid (hesperidin) as model reaction, this communication explores the use of (non-hazardous and biodegradable) deep eutectic solvents (DESs) as co-solvents in flavonoid biocatalysis. The enzymatic deglycosylation was observed when DES composed of choline chloride and glycerol or ethylene-glycol was used at proportions of up to 40% (DES-Buffer, v\/v), displaying a promising framework to combine enhanced flavonoid solubilities and high enzymatic activities. The deglycosylation activity significantly increased when the single DES components ? glycerol and ethylene-glycol ? were added (e.g. 140% of enzyme activity at glycerol at 40% v\/v), whereas deleterious effects were observed when choline chloride was solely added, presumably due to its chaotropic effect. Future research opportunities may be envisaged in the genetic design to evolve more robust biocatalysts, and in tailoring DES to deliver more enzyme-compatible solvents.","palabras_clave":"Flavanone glycoside  |Glycerol | Ethylene glycol  |DES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6135,"idioma_id":4,"titulo":"A model for the non-thermal emission of the very massive colliding-wind binary HD 93129A","resumen":"Context. Recently, the colliding-wind region of the binary stellar system HD 93129A was resolved for the first time using Very Large Baseline Interferometry. This system, one of the most massive known binaries in our Galaxy, presents non-thermal emission in the radio band, which can be used to infer the physical conditions in the system, and make predictions for the high-energy band. Aims: We intend to constrain some of the unknown parameters of HD 93129A through modeling the non-thermal emitter. We also aim to analyse the detectability of this source in hard X-rays and &#947;-rays. Finally, we want to predict how the non-thermal emission will evolve in the future, when the stars approach periastron. Methods: A broadband radiative model for the wind-collision region (WCR) has been developed taking into account the evolution of the accelerated particles streaming along the shocked region, the emission by different radiative processes, and the attenuation of the emission propagating through the local matter and radiation fields. We reproduce the available radio data, and make predictions of the emission in hard X-rays and &#947;-rays under different assumptions. Results: From the analysis of the radio emission, we find that the binary HD 93129A is more likely to have a low inclination and a high eccentricity, with the more massive star being currently closer to the observer. The minimum energy of the non-thermal electrons seems to be between ~20-100 MeV, depending on the intensity of the magnetic field in the WCR. The latter can be in the range ~20-1500 mG. Conclusions: Our model is able to reproduce the observed radio emission, and predicts that the non-thermal radiation from HD 93129A will increase in the near future. With instruments such as NuSTAR, Fermi, and CTA, it will be possible to constrain the relativistic particle content of the source, and other parameters such as the magnetic field strength in the WCR which, in turn, can be used to obtain upper-limits of the magnetic field on the surface of the very massive stars, thereby inferring whether magnetic field amplification is taking place in the particle acceleration region.","palabras_clave":"stars: massive|radiation mechanisms: non-thermal|acceleration of particles|stars: winds, outflows"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6136,"idioma_id":5,"titulo":"El juego desde los jugadores: huellas en Huizinga y Caillois","resumen":"Tanto al juego como a la cultura les es posible evolucionar tanto hacia la seriedad y funcionalidad absoluta (esto es lo que ha estudiado Caillois y han seguido Elías y Dunning) como hacia la gratuidad más sentida, hacia la creación de un nuevo sentido a acciones cotidianas, hacia la diversión, (postura construida desde una amalgama de autores, entre ellos, Pavía, Mantilla, Scheines, Ambrosini).   ¿Es posible una complejización de lo lúdico evitando el desplazamiento de la ética del juego (que instala un orden desordenado que se disfruta) a la lógica (que instala un orden extrínseco al juego que lleva a padecer la incertidumbre)?, ¿puede ser eso enseñado? En otras palabras, ¿es posible enseñar a complejizar lo lúdico buscando simplemente divertirse con otros (evitando la irrupción de la seriedad, ligada a la utilidad)? Estas preguntas inspiran la línea de estudio del juego desde los jugadores, cuyas huellas se rastrean en las obras Homo Ludens de Huizinga, y Los juegos y los hombres de Caillois.","palabras_clave":"JUEGO|LUDICIDAD|DIVERSION|ANTECEDENTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6137,"idioma_id":4,"titulo":"A new lizard species of the  Phymaturus patagonicus  group   (Squamata: Liolaemini) from northern Patagonia, Neuquén, Argentina","resumen":"The integrative taxonomy framework allows developing robust hypotheses of species limits based on the integration of results from different data sets and analytical methods. In this work, we test a candidate species hypothesis previously suggested based on molecular data, with geometric and traditional morphometrics analyses (multivariate and univariate). This new lizard species is part of the Phymaturus patagonicus group (payuniae clade) that is distributed in Neuquén and Mendoza provinces (Argentina). Our results showed that Phymaturus rahuensis sp. nov. differs from the other species of the payuniae clade by a higher number of midbody scales, and fewer supralabials scales, finger lamellae and toe lamellae. Also, its multidimensional spaces, both based on continuous lineal variables and geometric morphometrics (shape) characters, do not overlap with those of the other species in this clade. The results of the morphometric and geometric morphometric analyses presented here, coupled with previously published molecular data, represent three independent lines of evidence that support the diagnosis of this new taxon.","palabras_clave":"ARGENTINA|IGUANIDAE SENSU LATO|LIOLAEMINI|PATAGONICUS GROUP|PHYMATURUS RAHUENSIS SP. NOV.|NEUQUÉN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6138,"idioma_id":4,"titulo":"Reproductive pattern of the aggressive invader  Limnoperna fortunei (Bivalvia, Mytilidae) in South America","resumen":"Limnoperna fortunei is an aggressive freshwater invader species recorded for the first time in 1991 at Balneario Bagliardi, Río de la Plata Estuary, Argentina. Since then it has spread rapidly within South America. We tested the hypothesis that invasive species need a time span for the development of a reproductive pattern once settled in a new environment. We analyzed the reproductive cycle of a population of L. fortunei from Guaiba Lake (29°55'S; Rio Grande do Sul, Brazil) and compared it with previous studies at Balneario Bagliardi (34°52'S). We studied 834 specimens from Guaiba, following routine histological techniques. At any sampled period, the highest proportion of specimens were mature and gonads showed no resting periods. Female gonad maturation periods and oocyte growth were recorded throughout the year. Five periods of spawning of different intensity were differentiated. The reproductive pattern of the Guaiba population showed more spawning events and a remarkable recovery capacity of the female gonad compared to the Balneario Bagliardi population. The reproductive patterns of L. fortunei from Guaiba two years after invasion shows similarities with the reproductive pattern described previously from Balneario Bagliardi after two and three years of invasion.","palabras_clave":"REPRODUCTIVE PATTERN|INVASION|FRESHWATER BIVALVE|NEOTROPICAL REGION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6139,"idioma_id":4,"titulo":"VOCS ABATEMENT IN ADIABATIC MONOLITHIC REACTORS: HEAT EFFECTS, TRANSPORT LIMITATIONS AND DESIGN CONSIDERATIONS","resumen":"AbstractA ready-made MnCu catalyst showing a good performance in the combustion of volatile organic compounds has been deposited on FeCrAlloy® metallic monoliths by means of washcoating. With the aim of depositing a homogeneous and well-adhered layer of catalyst on the monoliths, the effects of a pre-coating with colloidal alumina as a primer, the solid concentration in the slurry, the addition of a stabilizer to the slurry, and the immersions number of the monolith in the slurry were studied. The addition of a stabilizer to the slurry was necessary to prevent sedimentation and to achieve reproducible washcoatings. The pre-coating of the monolith with the primer improved the washcoating adherence obtained with one impregnation but a better adherence did not compensate a lower solid load due to a lower surface roughness. A higher amount of deposited material was obtained with the slurry at 35&nbsp;wt% making two impregnations. The obtained monoliths showed excellent catalytic activity in the combustion of ethyl acetate and toluene. The activity as well as the surface area and pore volume of each monolith increased with the amount of retained solid.","palabras_clave":"VOC|MONOLITHIC REACTOR|COMBUSTION|MODELING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6140,"idioma_id":4,"titulo":"Effect of ionic liquid on the thermal decomposition of cyclic organic peroxides","resumen":"The thermolysis of cyclic diethylketone triperoxide (3,3,6,6,9,9-hexaethyl-1,2,4,5,7,8-hexaoxacyclononane, DEKTP) and cyclic pinacolone diperoxide (3,6-diterbutyl-3,6-dimethyl-1,2,4,5-tetraoxacyclohexane, PDP) was studied in neat N,N-dimethylformamide (DMF) and in binary mixtures DMF\/Ionic Liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [BMIM+][BF4&#8722;]). The activation parameters for the decomposition of both peroxides in DMF, correspond to a process of homolytic cleavage of one Osingle bondO bond, generating a biradical in the initial step of radical mechanism. The rate constant values (kd) are higher for reactions performed in mixtures. A gradual increase of [BMIM+][BF4&#8722;] in the reaction media generates higher kd for both peroxides (i.e. kd, DEKTP in DMF\/[BMIM+][BF4&#8722;] 4.0 mol L&#8722;1 is 3.3 times greater than the kd obtained for the mixture with a concentration 1.0 mol L&#8722;1, under the same experimental conditions). The increase in decomposition rate can be associated with a more solvated and stabilized transition state when the ionic liquid is part of the reaction medium.","palabras_clave":"ionic liquid|solvent effect|thermolysis|cyclic organic peroxides|isothermal kinetic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6141,"idioma_id":4,"titulo":"Beach carrying capacity assessment through image processing tools for coastal management","resumen":"Coastal environments are spaces where people may develop varied economic and recreational activities, such as tourism, which usually damage beaches and other natural resources commonly placed in these settings. The aim of this paper is to present a methodology to estimate and evaluate the Beach Carrying Capacity (BCC) and the actual beach usage level in coastal cities, using on-site information and video processing to provide significant real-time data. To test our methodology, we chose the coastal city of Monte Hermoso, Argentina, as a pilot site because it is by far the prime choice for a large population during summer vacation in this country. Initially, to estimate BCC, cartographic information about facilities and beach zones was collected and combined with surveys requested to tourists, to better understand their habits and preferences. This allowed an accurate estimation of other beach capacities related to BCC. Secondly, beach video sequences were processed with an algorithm that identified, located and counted people on the beach with an adequate accuracy. The actual occupancy factor was computed and used to asses whether the BCC had been exceeded. Also, people were tracked and their preferred relaxing areas were registered (e.g, closer to seaside during the morning). Finally, all the information was stored and visualized using a Geographic Information System (GIS) which allows both to analyze the different information layers and to produce interactive thematic maps. In this way, the resulting methodology may help to identify zones under risk of deterioration and to define suitable places for the development of varied activities (specially those related to tourism). It may also serve as a dashboard for decision and policy making and contribute to coastal management planning as well.","palabras_clave":"Procesamiento Digital de Imágenes|Sistemas de Información Geográfica|Manejo costero"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6142,"idioma_id":4,"titulo":"Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells","resumen":"Abstract The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficultto-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer\/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 was significantly enhanced after magnetofection with RAd-MNPs complexes in C2C12 myotubes in vitro and mouse skeletal muscle in vivo when compared to transduction with naked virus. These results provide evidence that magnetofection, mainly due to its membrane-receptor independent mechanism, constitutes a simple and effective alternative to current methods for gene transfer into traditionally hard-to-transfect biological models.","palabras_clave":"MAGNETOFECTION|ADENOVIRAL VECTORS|GENE THERAPY|SKELETAL MUSCLE CELLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6143,"idioma_id":4,"titulo":"A novel set of Bilateral and Radial Symmetry Shape Descriptor based on contour information","resumen":"Form and shape descriptors are among the most useful features for object identification13 and recognition. Even though there exists a widely used set of shape descriptors and underlying14 computational methods for their evaluation, frequently they fail to distinguish among very similar15 objects that they appear very different to the human eye. We propose a new set of shape16 descriptors based on a finer determination of the object symmetry axes, and a more accurate17 estimation of the bilateral and radial symmetry. These descriptors were thoroughly tested using18 several synthetic and real objects with varying degrees of symmetry. The methods for axes19 estimation and symmetry descriptors extraction outperform the widespread shape descriptors in20 recognizing and identifying among very similar objects.","palabras_clave":"Procesamiento Digital de Imágenes|Descriptores de forma|Análisis de Simetría"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6144,"idioma_id":4,"titulo":"Electrical Characterization of Semiconductor Oxide-Based Gas Sensors Using Impedance Spectroscopy: A Review","resumen":"Impedance spectroscopy is essential in understanding the physical and chemical processes that affect the electronic behavior of semiconducting metal oxide-based gas sensor materials, but is often overlooked or avoided by researchers because of the complexity of proper data interpretation. These metal oxide nanomaterials are promising as resistive-type gas sensors because they have the potential to meet the critical need that has developed for low-power, low-cost, portable gas sensors. However, the mechanisms that determine these materials? gas-sensing performance is still not understood well enough to advance the field toward bottom-up design for specific applications. Direct current (DC) measurements give information on the device performance such as sensitivity, selectivity and response time. Alternating current (AC) measurements are a lesser-used approach that can give the same information, but also allow the varying contributions from the bulk, surfaces and interfaces, grain boundaries, electrode contacts and even substrate to be quantified, whether we work with polycrystalline materials or single-crystal structures such as nanowires and nanorods. With this technique it is possible to extract fundamental information about intergranular potential energy barrier height, width and dono concentration changes in different atmospheres and temperatures. The equivalent circuit method is the most common method of relating the impedance data to the physical system. However, the main weakness of this method is that the ideal circuit elements used in the fitting can be connected in many different ways to fit the same signal. To properly apply this technique, the model development should consider both the impedance measurement and the physical and chemical characteristics of the system being studied. This review presents a detailed description of impedance spectroscopy techniques for fundamental analysis of materials behavior in gas sensing applications, including proper data interpretation and types of fitting models commonly used, to enable researchers to better apply these techniques toward understanding of their materials systems.","palabras_clave":"OXIDE-BASED GAS SENSORS|IMPEDANCE SPECTROSCOPY|CONDUCTION MECANISMS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6145,"idioma_id":5,"titulo":"Relación estructural entre la Precordillera Central y Occidental en la sección del Río San Juan","resumen":"El límite entre la Precordillera Central y Occidental en la sección del Río San Juan es una estructura compleja debido a la superposición de dos generaciones de corrimientos. El corrimiento mas antiguo transportó las facies desorganizadas del talud continental (Formación Los Sombreros), pertenecientes a la Precordillera Occidental, sobre las facies de plataforma de la Precordillera Central. Este corrimiento se emplazó durante la deformación Chánica, dado que depósitos discordantes de edad Carbonífera truncan la superficie de corrimiento. Tanto esta superficie como los depósitos carboníferos están cortados por un corrimiento de alto ángulo desarrollado en fuera de secuencia, el cual da lugar a la duplicación de la Precordillera Central y Occidental, del mismo modo que las unidades estratigráficas se repiten en los mapas por un primer sistema de corrimientos. La duplicación de la Precordillera Central y Occidental modifica la disposición paleogeográfica inicial, produciendo una inversión paleogeográfica aparente en el mapa geológico.","palabras_clave":"Precordillera Argentina|corrimientos fuera de secuencia|inversión paleogeográfica|Formación Los Sombreros|Paleozoico Inferior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6146,"idioma_id":4,"titulo":"Exploring genotype, management, and environmental variables influencing grain yield of late-sown maize in central Argentina","resumen":"Maize is one of the most important crops worldwide. The analysis of the influences of genotype, management, and environmental variables on grain yield has important consequences for guiding farmer?s decisions.Argentina is facing relevant changes in its production system, as farmers are planting later in the growing season. It is unclear, however, which management decisions are critical, and how they interact with contrasting genotypes. Using mixed-effects models we analyzed the influences of different genotypes, management, environmental predictors and relevant two-way interactions between these predictors on grain yield in late-sown maize. On-farm multi-environmental trials were conducted during two years (2013 and 2014), with a total of 9 genotypes tested at 23 different environments in the central region of Argentina. The influence of management variables like planting date, stand density, N availability, and soil P were explored. Similarly, we analyzed the influence of environmental variables like soil type, rainfall during the crop cycle, and the presence of an influencing water table.Averaged grain yield varied from 5,555 to 12,078 kg ha&#8722;1 among environments. Our best model described the spatial and temporal variation in grain yield (r2 = 0.91). Genotypes varied in their performance across environments and evidenced significant interaction with N availability. Management variables positively influencing yield were, in order of relevance, N availability and stand density. N availability had a positive decelerating effect, with an initial slope of 22 kg ha&#8722;1 per additional kg N ha &#8722;1. Increasing the stand density had a positive linear effect of 1,001 kg ha&#8722;1 per additional increment of 10,000 pl ha&#8722;1 (from 54,000 to 76,000 pl ha&#8722;1 explored range). Presence of an influencing water table at planting had a negative effect on yield (&#8722; 1,361 kg ha&#8722;1), suggesting that water availability could be in excess in later plantings. We demonstrated that, across a wide variability in soil types and rainfall, maize grain yield can be increased by choosing superior, high responsive genotypes, increasing stand density and applying optimal N rates. Results have important implications for guiding maize management and highlight that effective decisions require the combination of management options.","palabras_clave":"maize|yield|management|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6147,"idioma_id":4,"titulo":"Deep Learning for plant identification using vein morphological patterns","resumen":"We propose using a deep convolutional neural network (CNN) for the problem of plant identification from leaf vein patterns. In particular, we consider classifying three different legume species: white bean, red bean and soybean. The introduction of a CNN avoids the use of handcrafted feature extractors as it is standard in state of the art pipeline. Furthermore, this deep learning approach significantly improves the accuracy of the referred pipeline. We also show that the reported accuracy is reached by increasing the model depth. Finally, by analyzing the resulting models with a simple visualization technique, we are able to unveil relevant vein patterns.","palabras_clave":"DEEP LEARNING|MACHINE VISION|AUTOMATIC PLANT IDENTIFICATION|LEAF VEIN IMAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6148,"idioma_id":4,"titulo":"Modeling and simulation-driven engineering based on discrete-event systems specifications","resumen":"Robust engineering methodologies offering productlifecycle control have proved to be a cornerstone inmodern software development projects. Simulta-neously, various modeling and simulation (M&S)techniques have become increasingly adopted in complexsystem design, particularly in scenarios in which it?s difficultto predict system behavior as changes are introduced.The DEVS (Discrete Event Systems Specification) frame-work is the most general formalism for modeling discreteevent systems 1?3 and has been adopted in several disciplinesfor complex software and hardware system design and analy-sis. 4,5 In addition to providing an unambiguous mathemati-cal formalism to define model behavior and structure, DEVS70 Computing in Science & Engineeringprovides a clear framework for system analysis, experimentalframe definition, model-to-simulator verification, and mod-el-to-system validation.We present a DEVS-based methodology for M&S-driv-en engineering projects that integrates software developmentbest practices tailored to a large-scale networked data acquisi-tion system in a physics experiment (specifically, the ATLASparticle detector 6 at CERN 7 ). This project poses M&S chal-lenges from several viewpoints, including system complexity,tight delivery times, the quality and flexibility of the devel-oped models and tools, interdisciplinary communication ofresults to collaborators (mostly scientists), and big data-scaleanalysis.","palabras_clave":"Simulacion|devs|proceso|cern"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6149,"idioma_id":4,"titulo":"'Reversible redox modifications of ryanodine receptor ameliorate ventricular arrhythmias in the ischemic-reperfused heart'","resumen":"Previous results from our laboratory showed that phosphorylation of ryanodine receptor 2 (RyR2) by Ca(2+) calmodulin-dependent kinase II (CaMKII) was a critical but not the unique event responsible for the production of reperfusion-induced arrhythmogenesis, suggesting the existence of other mechanisms cooperating in an additive way to produce these rhythm alterations. Oxidative stress is a prominent feature of ischemia\/reperfusion injury. Both CaMKII and RyR2 are proteins susceptible to alteration by redox modifications. This study was designed to elucidate whether CaMKII and RyR2 redox changes occur during reperfusion and whether these changes are involved in the genesis of arrhythmias. Langendorff-perfused hearts from rats or transgenic mice with genetic ablation of CaMKII phosphorylation site on RyR2 (S2814A) were subjected to ischemia-reperfusion in the presence or absence of a free radical scavenger (mercaptopropionylglycine, MPG) or inhibitors of NADPH oxidase and nitric oxide synthase. Left ventricular contractile parameters and monophasic action potentials were recorded. Oxidation and phosphorylation of CaMKII and RyR2 were assessed. Increased oxidation of CaMKII during reperfusion had no consequences on the level of RyR2 phosphorylation. Avoiding the reperfusion-induced thiol oxidation of RyR2 with MPG produced a reduction in the number of arrhythmias and did not modify the contractile recovery. Conversely, selective prevention of S-nitrosylation and S-glutathionylation of RyR2 was associated with higher numbers of arrhythmias and impaired contractility. In S2814A mice, treatment with MPG further reduced the incidence of arrhythmias. Taken together, the results suggest that redox modification of RyR2 synergistically with CaMKII phosphorylation modulates reperfusion arrhythmias.","palabras_clave":"ARRITMIAS|REPERFUSION|REDOX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6150,"idioma_id":4,"titulo":"A prime-boost immunization with Tc52 N-terminal domain DNA and the recombinant protein expressed in Pichia pastoris protects against Trypanosoma cruzi infection","resumen":"We have previously reported that the N-terminal domain of the antigen Tc52 (NTc52) is the section of the protein that confers the strongest protection against Trypanosoma cruzi infection. To improve vaccine efficacy, we conducted here a prime-boost strategy (NTc52PB) by inoculating two doses of pcDNA3.1 encoding the NTc52 DNA carried by attenuated Salmonella (SNTc52), followed by two doses of recombinant NTc52 expressed in Picchia pastoris plus ODN-CpG as adjuvant. This strategy was comparatively analyzed with the following protocols: (1) two doses of NTc52+ODN-CpG by intranasal route followed by two doses of NTc52+ODN-CpG by intradermal route (NTc52CpG); (2) four doses of SNTc52; and (3) a control group with four doses of Salmonella carrying the empty plasmid. All immunized groups developed a predominant Th1 cellular immune response but with important differences in antibody development and protection against infection. Thus, immunization with just SNTc52 induces a strong specific cellular response, a specific systemic antibody response that is weak yet functional (considering lysis of trypomastigotes and inhibition of cell invasion), and IgA mucosal immunity, protecting in both the acute and chronic stages of infection. The group that received only recombinant protein (NTc52CpG) developed a strong antibody immune response but weaker cellular immunity than the other groups, and the protection against infection was clear in the acute phase of infection but not in chronicity. The prime-boost strategy, which combines DNA and protein vaccine and both mucosal and systemic immunizations routes, was the best assayed protocol, inducing strong cellular and humoral responses as well as specific mucosal IgA, thus conferring better protection in the acute and chronic stages of infection.","palabras_clave":"Picchia pastoris|Salmonella enterica|DNA-delivery system|Prime-boost|Chagas disease"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6151,"idioma_id":4,"titulo":"Sulfamide derivatives with selective carbonic anhydrase VII inhibitory action","resumen":"A set of N,N&#8242;-disubstituted sulfamides and sodium cyclamate have been tested for their inhibitory action against six isoforms of carbonic anhydrase (CA, EC 4.2.1.1) found in the brain, that is, CA I, CA II, CA VII, CA IX, CA XII and CA XIV, some of which are involved in epileptogenesis. The biological data showed interesting results for CA VII inhibition, the isozyme thought to be a novel antiepileptic target. Strong CA VII inhibitors, with Ki values in the low nanomolar?subnanomolar range were identified. Some of these derivatives showed selectivity for inhibition of CA VII versus the ubiquitous isoform CA II, for which the Ki values were in the micromolar range. Molecular modeling approaches were employed to understand the binding interactions between these compounds and the two CA isoforms, since the mechanism of action of such disubstituted sulfamides was not yet investigated by means of X-ray crystallography.","palabras_clave":"Carbonic anhydrase|Sulfamides|Docking|Epilepsy|Sodium cyclamate"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6152,"idioma_id":4,"titulo":"Understanding the Zr and Si interdispersion in Zr1&#8722;xSixO2 mesoporous thin films by using FTIR and XANES spectroscopy","resumen":"Zr?Si mixed mesoporous oxides were obtained in a wide range of proportions, from 0 to 30% and from 70 to 100% of Si, using Si(OEt)4 and ZrCl4 as precursors and Pluronic F127 as a template. The oxide mesostructure was characterized by transmission electron microscopy and 2D-small angle X-ray scattering.Fourier transform infrared spectroscopy measurements suggested a local homogeneous interdispersion of both cations. Further selective studies using X-ray Absorption Near Edge Structure (XANES) spectroscopy for separately Zr and Si local environments, allowed for demonstrating that the Zr coordinationvaries from close to 7 to 6, when its concentration in the mixed oxide is reduced. In addition, it was possible to determine that in mixed oxides with low Zr concentrations, Zr can fit into the spaces occupied by Si in SiO2 pure oxide. An equivalent XANES result was obtained for Si, which is also compatiblewith the information obtained by FTIR. Furthermore, the Zr?O distance varied from close to 2.2 Å to 1.7 Å when the Zr concentration decreased. Finally, our study also demonstrates the usefulness of XANES to selectively assess the local structure (coordination, symmetry and chemical state) of specific atoms innanostructured systems.","palabras_clave":"THIN FILMS|XANES|MESOPOROUS|ZIRCONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6153,"idioma_id":4,"titulo":"Patients with Mild Alzheimer?s Disease Fail When Using Their Working Memory: Evidence from the Eye Tracking Technique","resumen":"Patients with Alzheimer?s disease (AD) develop progressive language, visuoperceptual, attentional, and oculomotor changes that can have an impact on their reading comprehension. However, few studies have examined reading behavior in AD, and none have examined the contribution of predictive cueing in reading performance. For this purpose we analyzed the eye movement behavior of 35 healthy readers (Controls) and 35 patients with probable AD during reading of regular and high predictable sentences. The cloze predictability of words N? 1, and N+ 1 exerted an influence on the reader?s gaze duration. The predictabilities of preceding words in high-predictable sentences served as task-appropriate cues that were used by Control readers. In contrast, these effects were not present in AD patients. In Controls, changes in predictability significantly affected fixation duration along the sentence; noteworthy, these changes did not affect fixation durations in AD patients. Hence, only in healthy readers did predictability of upcoming words influence fixation durations via memory retrieval. Our results suggest that Controls used stored information of familiar texts for enhancing their reading performance and imply that contextual-word predictability, whose processing is proposed to require memory retrieval, only affected reading behavior in healthy subjects. In AD patients, this loss reveals impairments in brain areas such as those corresponding to working memory and memory retrieval. These findings might be relevant for expanding the options for the early detection and monitoring in the early stages of AD. Furthermore, evaluation of eye movements during reading could provide a new tool for measuring drug impact on patients? behavior.","palabras_clave":"Alzheimer´s Disease|Eye Movements|Memory|Reading"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6154,"idioma_id":5,"titulo":"De la velada de club a la estética de los cortejos. La construcción del 1° de Mayo socialista en la Argentina finisecular (1894-1900)","resumen":"Las celebraciones públicas como las del 1º de Mayo constituyen una instancia clave para abordar el fenómeno del origen y evolución de las identidades políticas modernas. En el artículo se propone reconstruir una serie de dimensiones fundamentales de la constitución de la «Fiesta del Proletariado» como el ritual político por excelencia del socialismo, al mismo tiempo que este comenzaba a dar forma a su organización partidaria en el país en la década de 1890. Teniendo en cuenta las inquietudes de los dirigentes y publicistas socialistas, las distintas formas que adquirió la celebración y los modelos que actuaron como fuentes, el 1º de Mayo se presenta como una demostración de la riqueza y complejidad de esta naciente cultura política en la Argentina finisecular.","palabras_clave":"PARTIDO SOCIALISTA|RITUALES POLÍTICOS|1° DE MAYO|IDENTIDADES POLÍTICAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6155,"idioma_id":4,"titulo":"Bilingualism and Cognitive Reserve: A Critical Overview and a Plea for Methodological Innovations","resumen":"The decline of cognitive skills throughout healthy or pathological aging can be slowed down by experiences which foster cognitive reserve (CR). Recently, some studies on Alzheimer?s disease have suggested that CR may be enhanced by life-long bilingualism.However, the evidence is inconsistent and largely based on retrospective approaches featuring several methodological weaknesses. Some studies demonstrated at least 4years of delay in dementia symptoms, while others did not find such an effect. Moreover, various methodological aspects vary from study to study. The present paper addresses contradictory findings, identifies possible lurking variables, and outlines methodological alternatives thereof. First, we characterize possible confounding factors that may have influenced extant results. Our focus is on the criteria to establish bilingualism, differences in sample design, the instruments used to examine cognitive skills, and the role of variables known to modulate life-long cognition. Second, we propose that these limitations could be largely circumvented through experimental approaches.Proficiency in the non-native language can be successfully assessed by combiningsubjective and objective measures; confounding variables which have been distinctively associated with certain bilingual groups (e.g., alcoholism, sleep disorders) can be targeted through relevant instruments; and cognitive status might be better tapped via robust cognitive screenings and executive batteries. Moreover, future research should incorporate tasks yielding predictable patterns of contrastive performance between bilinguals and monolinguals. Crucially, these include instruments which reveal bilingual disadvantages in vocabulary, null effects in working memory, and advantages in inhibitory control and other executive functions. Finally, paradigms tapping proactive interference(which assess the disruptive effect of long-term memory on newly learned information) could also offer useful data, since this phenomenon seems to be better managed by bilinguals and it becomes conspicuous in early stages of dementia. Such considerations may shed light not just on the relationship between bilingualism and CR, but also on more general mechanisms of cognitive compensation.","palabras_clave":"BILINGUALISM|ALZHEIMERS DISEASE|COGNITIVE RESERVE|RETROSPECTIVE STUDIES|EXPERIMENTAL RESEARCH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6156,"idioma_id":4,"titulo":"The combination of bleomycin with suicide or interferon-B gene transfer is able to efficiently eliminate human melanoma tumor initiating cells","resumen":"We explored the potential of a chemogene therapy combination to eradicate melanoma tumor initiating cells, key producers of recurrence and metastatic spread. Three new human melanoma cell lines, two obtained from lymph nodes and one from spleen metastasis were established and characterized. They were cultured as monolayers and spheroids and, in both spatial configurations they displayed sensitivity to single treatments with bleomycin (BLM) or human interferon-&#946; (hIFN&#946;) gene or herpes simplex virus thymidine kinase\/ganciclovir suicide gene (SG) lipofection. However, the combination of bleomycin with SG or hIFN&#946; gene transfer displayed greater antitumor efficacy. The three cell lines exhibited a proliferative behavior consistent with melan A and gp100 melanoma antigens expression, and BRAF V600E mutation. BLM and both genetic treatments increased the fraction of more differentiated and treatment-sensitive cells. Simultaneously, they significantly decreased the sub-population of tumor initiating cells. There was a significant correlation between the cytotoxicity of treatments with BLM and gene transfer and the fraction of cells exhibiting (i) high proliferation index, and (ii) high intracellular levels of reactive oxygen species. Conversely, the fraction of cells surviving to our treatments closely paralleled their (i) colony and (ii) melanosphere forming capacity. A very significant finding was that the combination of BLM with SG or hIFN&#946; gene almost abrogated the clonogenic capacity of the surviving cells. Altogether, the results presented here suggest that the combined chemo-gene treatments are able to eradicate tumor initiating cells, encouraging further studies aimed to apply this strategy in the clinic.","palabras_clave":"INTERFERON-B|TUMOR INITIATING CELLS|GENE THERAPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6159,"idioma_id":4,"titulo":"Entanglement breaking channels and entanglement sudden death","resumen":"The occurrence of entanglement sudden death in the evolution of a bipartite system depends on both the initial state and the channel responsible for the evolution. An extreme case is that of entanglement-breaking channels, which are channels that, acting on only one of the subsystems, drives them to full disentanglement, regardless of the initial state. In general, one can find certain combinations of initial states and channels acting on one or both subsystems that can result in entanglement sudden death or not. Neither the channel nor the initial state are responsible for this effect but their combination. In this paper we show that, for two entangled qubits, when entanglement sudden death occurs, the evolution can be mapped to that of an effective entanglement-breaking channel on a modified initial state. Our results allow to anticipate which states will suffer entanglement sudden death or not for a given evolution. An experiment with polarization-entangled photons demonstrates the utility of this result in a variety of cases.","palabras_clave":"óptica cuántica|física cuántica|entrelazamiento"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6160,"idioma_id":4,"titulo":"The 'indian glasses'","resumen":"Síntesis  El articulo Los vidrios indios trata acerca de la expansión de las potencias del capitalismo industrial y la distribución de los objetos de la cultura europea en áreas de las colonias americanas, en particular en las regiones de Pampa y Patagonia argentinas. Asimismo detalla como fueron tallados con tecnología indígena esas sustancias de origen europeo.   Enviado a revisión a Internacional Journal of Historical Archaeology.   Editor: Charles Orser.   REFERATO: SÍ.   University of California.","palabras_clave":"Pampa|Siglos XVIII y XIX|Vidrios tallados|Pueblos originarios"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6161,"idioma_id":4,"titulo":"Results of the engineering run of the Coherent Neutrino Nucleus Interaction Experiment (CONNIE)","resumen":"The CONNIE detector prototype is operating at a distance of 30 m from the core of a 3.8 GWth nuclear reactor with the goal of establishing Charge-Coupled Devices (CCD) as a new technology for the detection of coherent elastic neutrino-nucleus scattering. We report on the results of the engineering run with an active mass of 4 g of silicon. The CCD array is described, and the performance observed during the first year is discussed. A compact passive shield was deployed around the detector, producing an order of magnitude reduction in the background rate. The remaining background observed during the run was stable, and dominated by internal contamination in the detector packaging materials. The in-situ calibration of the detector using X-ray lines from fluorescence demonstrates good stability of the readout system. The event rates with the reactor ON and OFF are compared, and no excess is observed coming from nuclear fission at the power plant. The upper limit for the neutrino event rate is set two orders of magnitude above the expectations for the standard model. The results demonstrate the cryogenic CCD-based detector can be remotely operated at the reactor site with stable noise below2 e RMS and stable background rates. The success of the engineering test provides a clear path for the upgraded 100 g detector to be deployed during 2016.","palabras_clave":"Detector design and construction technologies and materials|Solid state detectors|Neutrino detectors"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6162,"idioma_id":4,"titulo":"Unified entropic measures of quantum correlations induced by local measurements","resumen":"We introduce quantum correlation measures based on the minimal change in unified entropies induced by local rank-one projective measurements, divided by a factor that depends on the generalized purity of the system in the case of nonadditive entropies. In this way, we overcome the issue of the artificial increasing of the value of quantum correlation measures based on nonadditive entropies when an uncorrelated ancilla is appended to the system, without changing the computability of our entropic correlation measures with respect to the previous ones. Moreover, we recover as limiting cases the quantum correlation measures based on von Neumann and Rényi entropies (i.e., additive entropies), for which the adjustment factor becomes trivial. In addition, we distinguish between total and semiquantum correlations and obtain some inequalities between them. Finally, we obtain analytical expressions of the entropic correlation measures for typical quantum bipartite systems.","palabras_clave":"Quantum correlations|Quantum unified entropies|Local projective measurements"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6163,"idioma_id":4,"titulo":"Comment on 'Quantum Kaniadakis entropy under projective measurement'","resumen":"We comment on the main result given by Ourabah et al. [Phys. Rev. E 92, 032114 (2015)], noting that it can be derived as a special case of the more general study that we have provided in [Quantum Inf Process 15, 3393 (2016)]. Our proof of the nondecreasing character under projective measurements of so-called generalized (h,&#966;) entropies (that comprise the Kaniadakis family as a particular case) has been based on majorization and Schur-concavity arguments. As a consequence, we have obtained that this property is obviously satisfied byKaniadakis entropy but at the same time is fulfilled by all entropies preserving majorization. In addition, we have seen that our result holds for any bistochastic map, being a projective measurement a particular case. We arguehere that looking at these facts from the point of view given in [Quantum Inf Process 15, 3393 (2016)] not only simplifies the demonstrations but allows for a deeper understanding of the entropic properties involved.","palabras_clave":"GENERALIZED ENTROPIES|SCHUR-CONCAVITY|MAJORIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6164,"idioma_id":4,"titulo":"KINETIC MODEL OF WATER DISINFECTION USING  PERACETIC ACID INCLUDING SYNERGISTIC EFFECTS","resumen":"The disinfection efficiencies of a commercial mixture of peracetic acid against Escherichia coli were studied in laboratory scale experiments. The joint and separate action of two disinfectant agents, hydrogen peroxide and peracetic acid, were evaluated in order to observe synergistic effects. A kinetic model for each component of the mixture and for the commercial mixture was proposed. Through simple mathematical equations, the model describes different stages of attack by disinfectants during the inactivation process. Based on the experiments and the kinetic parameters obtained, it could be established that the efficiency of hydrogen peroxide was much lower than that of peracetic acid alone. However, the contribution of hydrogen peroxide was very important in the commercial mixture. It should be noted that this improvement occurred only after peracetic acid had initiated the attack on the cell. This synergistic effect was successfully explained by the proposal scheme and was verified by experimental results. Besides providing a clearer mechanistic understanding of water disinfection such models may improve our ability to design reactors.","palabras_clave":"WATER DISINFECTION|PERACETIC ACID|KINETIC MODEL|SYNERGISM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6165,"idioma_id":4,"titulo":"Molecular and Kinetic Characterization of the Fermentative Behavior of Saccharomyces cerevisiae Strains Isolated from North Patagonia","resumen":"At present, Argentina does not count with a production of indigenous yeast strains with suitabletechnological and oenological features to be used in the regional winery industry. Isolation andmolecular characterization of these microorganisms and its fermentation attributes would be relevantto the sustainable development of the activity in the country and to recognize and preservethe biodiversity of the region. Eight strains isolated from grapes and musts from the North Patagonianregion and genetically identified as Saccharomyces cerevisiae, were studied for their fermentationbehavior, emphasizing in hexose transport through the plasma membrane, which is thelimiting step of the process. Thus, sugar consumption profiles were analyzed in different media atlaboratory scale, to be subsequently applied to the fermentation of natural musts. Three of theeight initial strains were selected, named ÑNM10, ÑIF8 and ÑMN16 according to their fermentationprofiles. The expression of hexose transporters during fermentations revealed interestingdifferences in the response of each strain to sugar consumption, where transporters HXT2 andHXT5 showed significant changes in expression in Patagonian strains, which are normally associatedto endurance to culture stress conditions. The results obtained by combining the characteristicsstudied, at molecular and physiological level, are extremely encouraging. Native strainÑMN16, showed a high potential for application in local winemaking. Assays carried out on a pilotscale will determine the feasibility of applying this strain with promising technological features atindustrial scale.","palabras_clave":"FERMENTATION|WINE|Saccharomyces cerevisiae|HEXOSE TRANSPORTERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6166,"idioma_id":4,"titulo":"Analysis of C9orf72 in patients with frontotemporal dementia and amyotrophic lateral sclerosis from Argentina","resumen":"Pathologic expansion of the G4C2 repeat in C9orf72 is the main genetic cause of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). To evaluate the frequency of the G4C2 expansion in a Latin American cohort of FTD and ALS patients, we used a 2-step genotyping strategy. For FTD, we observed an overall expansion frequency of 18.2% (6 of 33 unrelated cases). Moreover, the C9orf72 expansion accounted for 37.5% of all familial FTD cases (6 of 16 families). The expansion frequency in sporadic ALS cases was 2% (1 of 47 unrelated patients), whereas we observed the expansion in 1 of 3 families with a positive history for ALS. Overall, the expansion frequency in our FTD group was similar to that reported for patients in Europe and North America, whereas the frequency in our sporadic ALS group was significantly lower. To our knowledge, this is the first report on the frequency of the C9orf72 expansion in a Latin American population.","palabras_clave":"Amyotrophic lateral sclerosis|Dementia|C9orf72 |Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6167,"idioma_id":4,"titulo":"Approximate total Fe content determined by Mössbauer spectrometry: Application to determine the correlation between gamma-ray-emitter activities and total content of Fe phases in soils of the Province of Buenos Aires, Argentina","resumen":"Pearson correlation coef&#64257;cients between 40K,226Ra and 232Th activities and the total Fe phase fractions yielded by Mossbauer spectroscopy have been calculated for soils of the Province of Buenos Aires, Argentina. Total fractions of Fe phases have been obtained from the relative fractions reported in previousworks weighted by the Fe soil content and the recoilless-fraction of each Fe phase. An approximate method based on the relationship between the Mossbauer spectral absorption area (obtained from the 57Fe Mossbauer data) and the total Fe concentration (determined by colorimetric methods, after microwave assisted acid digestion of soil samples) has been used for the &#64257;rst time to determine the Feconcentration in soils with an accuracy of 15%. Protocol to extend the method for unknown samples is also discussed. The determined new coef&#64257;cients differ from those reported previously. A signi&#64257;cant and positive correlation between the total fraction of Fe2+ and the 40K activity values has been obtained. Thisresult validates the hypothesis put forward in a previous work, i.e., that illite captures the 40K existing in the soil. In addition, with the new approximation, the Pearson correlation coef&#64257;cients for the other natural radionuclides give values that indicate that the methodology reported here is appropriate tostudy the correlations between the activity values with the total fractions of Fe phases.","palabras_clave":"SOIL NATURAL RADIAOCTIVITY|TOTAL FE FRACTIONS|CORRELATION COEFFICIENTS|MOSSBAUER SPECTROSCOPY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6168,"idioma_id":4,"titulo":"Contribution of Linear Guest and Structural Pendant Chains to Relaxational Dynamics in Model Polymer Networks Probed by Time- Domain 1H NMR","resumen":"A series of end-linked polymer networks with varying contents of linear guest chains were investigated through swelling and time?domain NMR temperature dependent experiments. Taking advantage of the thermo-rheological simplicity of polydimethylsiloxane polymers, time?temperature superposition (TTS) was employed to expand the characteristic time scales of NMR exploration by about two orders of magnitude. A comparison between swelling data and tube models predictions reveals that NMR captures the dominant features of the equilibrium and the dynamic properties of defects trapped in polymer networks. As high-temperature experiments ensures a complete relaxation of the guest linear chains on the millisecond time scale of the NMR experiments, an accurate description of the network architecture can be provided. Contents of guest chains determined by NMR were found to agree within a 1wt.% accuracy with data of swelling experiments","palabras_clave":"relaxatyonal dynamics|model networks|guest chains|time domain H-NMR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6169,"idioma_id":5,"titulo":"Relación entre impulsividad y consumo de alcohol en hombres y  mujeres argentinos","resumen":"En  este  trabajo  analizamos,  separadamente  para  hombres  y  mujeres,  la  relación  entre  impulsividad y consumo de alcohol. Clasificamos a los participantes en tres clases de consumo de  alcohol  y  examinamos  diferencias  en  impulsividad  rasgo  entre  las  clases.  Participaron 435 jóvenes que completaron una encuesta online. A nivel bivariante, la relación entre los distintos indicadores de consumo de alcohol no fue la misma con todas las dimensiones de la UPPS-P.  En  general,  el  perfil  de  asociación  entre  las  variables  fue  similar  en  hombres  y  en mujeres. A nivel multivariante, se destaca la asociación de urgencia negativa (&#946; entre .24 y .34) y urgencia positiva (&#946; entre .15 y .28) sobre el consumo de alcohol y los problemas asociados.  Las  clases  mostraron  diferencias  en  el  perfil  de  impulsividad  subyacente  entre  las mujeres.  Las  mujeres  con  consumo  de  riesgo  son  más  impulsivas  que  sus  pares  con  menor consumo en las cinco dimensiones de la UPPS-P.","palabras_clave":"IMPULSIVIDAD|CONSUMO DE ALCOHOL|JÓVENES|PERFILES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6170,"idioma_id":4,"titulo":"Childhood poverty and cognitive development in Latin America in the 21st Century","resumen":"For at least eight decades, researchers have analyzed theassociation between childhood poverty and cognitive development in differentsocieties worldwide, but few such studies have been carried out in LatinAmerica. The aim of the present paper is to systematically review the empiricalstudies that have analyzed the associations between poverty and cognitivedevelopment in children under 18 years of age from Latin American and Caribbeancountries between 2000 and 2015. This analysis takes into consideration thecountry where the work was conducted, the design of the study and itsanalytical design, sample size and composition, cognitive and poverty paradigmsimplemented, levels of analysis considered, and the inclusion of mediationanalyses. Through these, we identify common patterns in the negative impact ofpoverty that have been repeatedly verified in the literature in othercontinents; we also&nbsp; call attention to aset of issues regarding sample, design, paradigms, impact, and mediationanalyses that should be considered in future studies in the region.","palabras_clave":"CHILDHOOD POVERTY|COGNITIVE DEVELOPMENT|LATIN AMERICA|SYSTEMATIC REVIEW"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6171,"idioma_id":5,"titulo":"Cuando lo privado se hace público: el abogado del niño en la justicia de familia","resumen":"El objetivo de este artículo es analizar las tensiones, disputas y conflictos que emergieron en el proceso de implementación del abogado del niño en la justicia de familia de la Argentina, específicamente en la Ciudad Autónoma de Buenos Aires y en la ciudad de La Plata. Para ello, a través del análisis de casos paradigmáticos -reconstruidos a partir del relevamiento de expedientes judiciales y de las charlas y entrevistas mantenidas con distintos actores en el transcurso de nuestro trabajo de campo- se indagan los diversos usos e interpretaciones que emergen con la entrada de esta figura en la escena judicial. Lejos de utilizar una lente jurídica para dar cuenta de los diferentes debates producidos a partir de este proceso de individualización de los niños en el ámbito judicial, en el cual han sido recortados como sujetos procesales con voz propia, abordamos esta novedosa figura desde una perspectiva socio-antropológica y examinamos dos tensiones recurrentes en los debates relativos a la garantía de los derechos de niños, niñas y adolescentes: la tensión entre lo público y lo privado, y la que se despliega en términos de autonomía vs. protección.","palabras_clave":"infancia|abogado del niño"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6172,"idioma_id":4,"titulo":"Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn","resumen":"The increase in carbapenem-resistant gram-negative bacteria is a matter of concern due to the limited therapeutic options available. In severe infections caused by these isolates, the rapid detection of the mechanisms of resistance is vital. We described a slightly modified version of the Blue-Carba test, rapid Blue-Carba test, which allows the detection of carbapenemases at 4&nbsp;h of incubation from a haze of bacterial growth obtained from a positive blood culture. It was able to detect carbapenemase-producing isolates (Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii) with a sensitivity and specificity of 98.1 and 100%, respectively. It is a rapid, easy-to-perform and an inexpensive technique that can be applied to routine laboratories, together with the simultaneous identification by mass spectrometry which would help to screen non-enzymatic carbapenem resistance; this method allows the detection of clinically relevant multidrug-resistant bacteria and the early implementation of accurate therapeutic interventions","palabras_clave":"Blood culture|Blue-Carba test|Carbapenemase; Detection time; Gram-negative bacteria; KPC|Detection time|Gram-negative bacteria|KPC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6173,"idioma_id":4,"titulo":"Oleaginous yeasts from Antarctica: screening and  preliminary approach on lipid accumulation","resumen":"The capability of 17 Rhodotorula spp. isolated from Antarctica to accumulate intracellular lipids in nitrogen-limited medium was investigated. As results, 10 isolates were selected by Nile red staining, while 12 isolates were selected as oleaginous by analysis of total lipid content (20.4?73%, w\/w of dry biomass). The higher lipid production and accumulation was exhibited for six strains belonging to three species of Rhodotorula (Rhodotorula glutinis, Rhodotorula glacialis, and Rhodotorula laryngis). This is the first report where R. laryngis have been identified within oleaginous specie. Lipid accumulation was evaluated comparatively in two nitrogen-limited glucose-based media (MI and MII). MI (low C\/N ratio) was more suitable for biomass and lipid production while in MII (high C\/N ratio) total lipid content was improved. R. glutinis R4, R. glacialis R15, and R. glutinis R48 showed high lipid concentrations (4.65?6.93&#8201;g&#8201;L&#8722;1) and they were able to accumulate large amounts of lipids per gram of biomass (47?77%, w\/w). A similar profile in fatty acids composition and content of neutral lipids to vegetable oils was observed, indicating that lipids produced by oleaginous Antarctic yeasts can be considered an alternative feedstock for biodiesel production. Antarctica represents an important source of oleaginous yeasts with adaptive capabilities to accumulate considerable amounts of lipids with biotechnological interest at 15&#8201;°C and 25&#8201;°C.","palabras_clave":"OLEAGINOUS YEASTS|MICROBIAL LIPIDS|BIODIESEL|ANTARCTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6174,"idioma_id":4,"titulo":"THE CONSERVATION STATUS AND PRIORITIES FOR ALBATROSSES AND LARGE PETRELS","resumen":"Seabirds are amongst the most globally-threatened of all groups of birds, and conservation issues specific to albatrosses (Diomedeidae) and large petrels (Procellaria spp. and giant petrels Macronectes spp.) led to drafting of the multi-lateral Agreement on the Conservation of Albatrosses and Petrels (ACAP). Here we review the taxonomy, breeding and foraging distributions, population status and trends, threats and priorities for the 29 species covered by ACAP. Nineteen (66%) are listed as threatened by IUCN, and 11 (38%) are declining. Most have extensive at-sea distributions, and the greatest threat is incidental mortality (bycatch) in industrial pelagic or demersal longline, trawl or artisanal fisheries, often in both national and international waters. Mitigation measures are available that reduce bycatch in most types of fisheries, but some management bodies are yet to make these mandatory, levels of implementation and monitoring of compliance are often inadequate, and there are insufficient observer programmes collecting robust data on bycatch rates. Intentional take, pollution (including plastic ingestion), and threats at colonies affect fewer species than bycatch; however, the impacts of disease and of predation by introduced species, including feral cats (Felis catus), rats (Rattus spp.) and house mice (Mus musculus), are severe for some breeding populations. Although major progress has been made in recent years in reducing bycatch rates and in controlling or eradicating pests at breeding sites, unless conservation efforts are intensified, the future prospects of many species of albatrosses and large petrels will remain bleak.","palabras_clave":"Albatrosses and Petrels|Biodiversity Conservation|Status and trends|Seabird bycatch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6175,"idioma_id":4,"titulo":"Novel mRNA-silencing bodies at the synapse: A never ending story","resumen":"Several cellular responses depend on translational regulation and in most cases, this involves theformation of cytoplasmic granules that contain repressed mRNAs. In neurons, numerous mRNAstravel along dendrites to be locally regulated upon synapse activity and we have recently shownthat the exoribonuclease XRN1 forms dynamic aggregates at the post synapse that respond tospecific stimuli.1 These foci were termed SX-bodies and are distinct from stress granules (SGs),processing bodies (PBs) and other RNA granules previously described. Together with Smaug1-fociand FMRP-granules, the SX-bodies contribute to dynamically shape the transcriptome available fortranslation at the post-synapse.","palabras_clave":"SILENCING BODIES|SYNAPSES|TRANSLATION REGULATION|mRNAs"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6176,"idioma_id":4,"titulo":"Hydrochemical and sedimentological dynamics in a subtropical plain river: assessment by multivariate statistical analysis","resumen":"Understanding the transport dynamics of sediments and soluble components is critical when ecosystems could be adversely affected. This study examined someenvironmental factors influencing the spatial and temporal variations of water?sediment interactions in Negro River,northeast of Argentina, a subtropical plain fluvial system with anthropogenic influence. Samples of water and bottomsediments were collected from four sampling stations in four sampling campaigns spread over 2 years. Chemical parameters, including pH, conductivity, major ions, concentration of suspended sediments, extractable major cations and particle size of sediments, were determined. The experimental data were interpreted by classical hydrogeochemical methods and statistical methods. Multivariate statistical tools were applied to reduce the dimensionality of the data set and to evaluate the relative importance of combinations of environmental variables on sediment dynamics. The results showed that the variability of the hydrochemistry and the sediment chemistry is controlledby longitudinal and seasonal factors, whereas differences across the river section were nonsignificant. The concentration of suspended solids and the abundance of fine-grained materials in bottom sediments were inverselycorrelated with the concentration of some metals with agglutinant and coagulant properties in sediments. This corroborates the presence of fine particle aggregates, also evidenced by classical grain-size analysis. Thus, particledisaggregation by chemical methods is not recommended,as it could lead to misinterpretation when the objective is to evaluate the dynamics of sediments in natural environments.This study highlights the importance of analyzingmultiple dimensions (spatial and temporal) to understand the dynamics of fluvial sediments, especially in plain rivers.","palabras_clave":"CHACO-ARGENTINA|TRANSPORT PROCESSES|PLAIN RIVERS|MULTIVARIATE ANALYSIS|PRINCIPAL COMPONENT ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6177,"idioma_id":4,"titulo":"Acute Effects of Light on Alternative Splicing in Light-Grown Plants","resumen":"Light modulates plant growth and development to a great extent by regulating gene expression programs. Here, we evaluated the effect of light on alternative splicing (AS) in light-grown Arabidopsis thaliana plants using high-throughput RNA sequencing (RNA-seq). We found that an acute light pulse given in the middle of the night, a treatment that simulates photoperiod lengthening, affected AS events corresponding to 382 genes. Some of these AS events were associated with genes involved in primary metabolism and stress responses, which may help to adjust metabolic and physiological responses to seasonal changes. We also found that several core clock genes showed changes in AS in response to the light treatment, suggesting that light regulation of AS may play a role in clock entrainment. Finally, we found that many light-regulated AS events were associated with genes encoding RNA processing proteins and splicing factors, supporting the idea that light regulates this posttranscriptional regulatory layer through AS regulation of splicing factors. Interestingly, the effect of a red-light pulse on AS of a gene encoding a splicing factor was not impaired in a quintuple phytochrome mutant, providing unequivocal evidence that nonphotosensory photoreceptors control AS in light-grown plants.","palabras_clave":"Splicing alternativo|Luz|Plantas|Fitocromos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6178,"idioma_id":4,"titulo":"Analysis of the transmission of Trypanosoma cruzi infection through hosts and vectors","resumen":"Calculating epidemiological measures of infection by Trypanosoma cruzi, the causative agent of Chagas disease, is complex, because it involves several species, different stages of infection in humans and multiple transmission routes. Using the next-generation matrix method, we analysed a model which considers the three stages of human infection, triatomines and dogs (the main domestic reservoirs of T. cruzi when triatomines are present) and the main transmission routes. We derived R 0 and type-reproduction numbers T. We deduced formulas for the number of new infections generated through each transmission route by each infected individual. We applied our findings in Argentine Gran Chaco. The expressions achieved allowed quantifying the high infectivity of dogs and emphasizing the epidemiological importance of the long and asymptomatic chronic indeterminate stage in humans in the spread of the infection. According to the model, it is expected that one infected human infects 21 triatomines, that 100 infected triatomines are necessary to infect one human and 34 to infect a dog, and that each dog infects on average one triatomine per day. Our results may allow quantifying the effect of control measures on infected humans, triatomines and dogs (or other highly infected vertebrate) or on a specific route of transmission, in other scenarios.","palabras_clave":"Trypanosoma cruzi|Chagas disease|mathematical model| next-generation matrix"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6179,"idioma_id":5,"titulo":"Arqueología y Etnohistoria: diálogos renovados en torno a las relaciones entre las sociedades de Los Llanos riojanos y de las Sierras Noroccidentales de Córdoba (períodos Prehispánico Tardío y Colonial Temprano).","resumen":"Este artículo propone integrar dos líneas de investigación independientes. Por un lado, la arqueológica, que comprende el estudio del arte rupestre prehispánico y colonial temprano en las regiones del Noroeste de Córdoba y de Los Llanos de La Rioja. Por otro la etnohistórica, centrada en las sociedades de Los Llanos durante el período del contacto hispano indígena. La discusión entre ambas permite cuestionar la aceptada homogeneidad étnica, lingüística y cultural de esta región y plantear en cambio un panorama diferente de diversidad lingüística y cultural, con estrechas vinculaciones de sus pobladores con los de las sierras noroccidentales de Córdoba (entre otros).","palabras_clave":"Arte rupestre|Etnohistoria|Los Llanos de La Rioja|Noroccidente de Córdoba"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6180,"idioma_id":4,"titulo":"Quantifying the influence of meteorological variables on particle-bound PAHs in urban environments","resumen":"Seasonal trend of polycyclic aromatic hydrocarbons (PAHs) associated with PM10 particles was assessed in an urban area in Cordoba, Argentina, during 2011-2013. In addition, we quantified the contribution of temperature, relative humidity, rainfall, atmospheric pressure and wind speed on total and individual PAH concentrations. Mean PAH concentrations were higher during autumn and winter. Temperature was the meteorological parameter that affected the most total and individual PAHs. Its influence was stronger in lightweight than in heavy PAHs, since they are more easily partitioned in the vapor phase. We found also that temperature has a lagged effect on PAHs concentration with the strongest association observed with 2-day lag temperature. Wind speed and relative humidity were also significant predictors for both light and heavy PAHs. Despite the fact that we observed a decreasing trend in the concentration levels ofPAHs, they are still high enough to pose a risk to human health due to chronic exposure.","palabras_clave":"Particulate phase PAHs|Córdoba|temperature|Seasonal variations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6181,"idioma_id":5,"titulo":"EMISIONES DE DIÓXIDO DE CARBONO ORIGINADAS POR EL CONSUMO DE ENERGÍA ELÉCTRICA EN EDIFICIOS DE LA PROVINCIA DE SAN JUAN-ARGENTINA","resumen":"Sin un uso consciente de la energía que consume, la población en crecimiento produce efectos irreversibles en el ambiente. La generación de energía con fuentes no renovables es responsable de impactos ambientales negativos por la emisión de gases de efecto invernadero (GEI). La concentración de dióxido de carbono (CO2) en la atmósfera es un indicador GEI. Este gas tiene la capacidad de almacenar la energía de radiación de onda larga (calor) en su molécula y evitar el equilibrio térmico natural entre la tierra y el espacio extraterrestre. El objetivo de este trabajo es encontrar un valor representativo de las emisiones de CO2 producidas por el consumo de energía eléctrica del sector edilicio (residencial, comercial y oficial) de la Provincia de San Juan (Argentina), y desarrollar una etiqueta gráfica para indicar el grado de emisiones. Para la evaluación, se consideran referencias internacionales en los valores de emisiones de CO2, datos de consumo eléctrico y datos de población censada. Se calculan, así, emis ones de CO2 per cápita, en función de los valores de consumo local y se procede a categorizar por equivalentes numéricos que se expresan en la etiqueta. Los valores de emisión de CO2-equivalente obtenidos resultan notablemente bajos en relación a la media mundial","palabras_clave":"consumo eléctrico|CO2-equivalente|etiqueta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6182,"idioma_id":4,"titulo":"Synthesis and Characterization of Latex-protein Complexes from Different Antigens of Toxoplasma gondii for Immunoagglutination Assays","resumen":"The acute phase recombinant protein of Toxoplasma gondii P22Ag was expressed and purified and thehomogenate of the parasite was obtained from an infected mouse. These antigens were used to producelatex-protein complexes (LPC) through physical adsorption and chemical coupling onto different latexes,with the aim of producing immunoagglutination (IA) reagents able to detect recently acquiredtoxoplasmosis. Polystyrene and ?core-shell? latexes where employed, exhibiting varied particle size,functionality (carboxyl or epoxy), and charge density. In sensitization experiments for producing LPC, therecombinant protein showed better coupling efficiency onto the particles surface than the homogenateand this could be explained by the complex mixture of the homogenate, which includes a large numberof proteins of different molecular mass, isoelectric points, and hydrophobicity. The synthesized LPC wereemployed in IA assays. To this effect, the agglutination reaction was followed by measuring the changesin the optical absorbance by turbidimetry. Experiments against control sera were performed to evaluatethe performance of various LPC and it was observed that the IA test based on P22Ag and thecarboxylated latex of 350 nm of particle diameter allowed a good discrimination between acute sera andchronic\/negative ones. The proposed test is cheap, rapid, and easy to implement.","palabras_clave":"TOXOPLASMOSIS|LATEX-PROTEIN COMPLEXES|IMMUNOAGGLUTINATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6184,"idioma_id":4,"titulo":"Early exposure to and subsequent beef cattle performance with saline water","resumen":"Two experiments were conducted to evaluate the impact of early life exposure to high salt water on later cattle performance with saline water. In Exp. 1, 24 cow\/calf pairs were randomly assigned to one of two treatments: exposure to high salt water [HSW; 7478 mg\/kg of total dissolved solids (TDS)] or to low salt water (LSW; 512 mg\/kg TDS) when calves were 2 to 6 mo. old. Then all calves drank low salt water for 6 mo, and subsequently high salt water for 30 d. During the last period HSW tended to eat 10% less DM (DMI; P = 0.07) and drank 22% less water than LSW (WI; P  0.13), plasma glucose (P = 0.18), serum minerals (P > 0.08) and weight gain (ADG; P = 0.85) were not affected by treatment. In Exp. 2, 24 pregnant heifers in the last month of gestation were randomly assigned to either HSW (10827 mg\/kg TDS) or LSW. The exposure period ended when calves were 3 month old. Then all calves drank low salt water for 95 d, and subsequently high salt water for 30 d. During the last period no significant differences between treatments were observed for DMI (P = 0.43), WI (P = 0.61), TTDMD (P = 0.92), blood parameters (P > 0.42), plasma rennin activity (PRA; P = 0.35), and ADG (P = 0.16). However, HSW drank less (P < 0.01) high salt water than LSW during the first two hours of drinking water restoration after a water deprivation period of 20 h. Overall, in the conditions of our study we did not find evidence that early exposure to saline water induces tolerance and improves later performance of beef cattle with salty water. However, reduced water intake (Exp. 1) and increased thirst threshold (Exp. 2) of animals early exposed to saline water need further consideration.","palabras_clave":"Beef cattle|Early experience|Plasma renin activity|Saline water tolerance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6185,"idioma_id":4,"titulo":"Texture and colour analysis of potato (Cv Spunta) for instant meal using instrumental and image analysis techniques","resumen":"An image based method was used to evaluate texture and colour in freeze dried potato. Samples were analyzed by Scanning electron microscopy; texture analyzer, spectrocolourimeter and by image analysis. Significant difference (P<0.05) were obtained for colour and texture. Principal component analysis and linear trend with PC`s scores was applied for instrumental vs. image features. Image texture (contrast, correlation, energy and homogeneity, calculated from GLCM) had high correlations with instrumental texture (hardness, adhesiveness, chewiness, cohesiveness and resilience; R2=0.842). Colour showed high correlations for instrumental vs. image (R2=0.823). With this approach to quality image processing techniques can be a useful tool.","palabras_clave":"Texture analysis|Color|Image analysis|potato quality"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6186,"idioma_id":4,"titulo":"D-Dimer as a prognostic marker of  morbidity and mortality among HIV patients: a call for attention","resumen":"Cardiovascular diseases (CVD) seem to be overrepresented among HIV-infected subjects and efforts are currently taken to find parameters useful for risk calculation. In the present journal, a recent study involved the ankle-branched index for screening of peripheral endothelial disease, however with negative outcome in terms of correlation with risk factors.[1] Recent work has been guided towards finding potential biomarkers and understanding the underlying pathophysiological processes that could help us to understand and predict the occurrence of these diseases.[2] As a result, D-dimer (DD) and interleukin-6 have emerged as potential key biomarkers of coagulation and inflammation processes, respectively, as they have been associated with a higher risk of fatal cardiovascular events and a higher risk of death after non-fatal cardiovascular events in a study that clustered three large cohorts of HIV patients.[3] Moreover, in a recent study, Borges et al. aimed to identify factors that were independently associated with DD levels in HIV patients and found a close association between DD levels with advancing age and suffering hepatic fibrosis.[4] Furthermore, it has also been proposed that a prospective HIV study should be conducted to establish a score based on genetic background and plasma biomarkers of coagulation, inflammation and endothelial function in order to predict cardiovascular events.[2] Therefore, if DD is considered a suitable candidate to be chosen as a biomarker of coagulation, we think that several important clinical and laboratory aspects of DD levels measurement should be considered, as they were overlooked in some studies. ","palabras_clave":"D DIMER|HIV|MARKER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6188,"idioma_id":5,"titulo":"Estudio de los restos arqueofaunísticos recuperados en el sitio Laguna del Telégrafo (Costa Norte de Santa Cruz)","resumen":"En este trabajo se presentan los resultados de los análisis realizados sobre los restos arqueofaunísticos, tanto de vertebrados e invertebrados, recuperados en el sitio Laguna del Telégrafo, ubicado en la localidad arqueológica Cabo Blanco (costa norte de Santa Cruz, Patagonia argentina). Este sitio se encuentra emplazado en el borde de una antigua planicie de mareas que actualmente constituye una laguna temporaria. Se trata de un conchero de pequeñas dimensiones en el cual se excavaron 2 m2 , y cuya antigüedad es de ca. 2300 años AP. Durante las tareas de excavación se identificó una lente de valvas en posición subsuperficial, en la cual se recuperaron gran cantidad de restos faunísticos (óseos y malacológicos), líticos y antracológicos, así como también un nudo de cuero. Se analiza y discute la posible funcionalidad del sitio y las características formacionales del depósito arqueológico.","palabras_clave":"Restos óseos|Restos malacológicos|Conchero|Costa Norte de Santa Cruz"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6189,"idioma_id":4,"titulo":"Non-pegmatitic beryl related to Carboniferous granitic magmatism, Velasco Range, Pampean Province, NW Argentina","resumen":"The specialized leuco-monzogranite of the La Chinchilla Stock is a small Carboniferous stock located in the center of the Velasco Range, Pampean Province, La Rioja, Argentina. It is highly evolved and locally F- and Be-bearing, and has the potential for hosting U mineralization. Three different facies can be identified in the granitoid: border, porphyritic and equigranular facies. In all three facies the main minerals are quartz, microcline, plagioclase, biotite, and muscovite. Accessory minerals present in all facies include fluorite, zircon, and apatite. In addition, monazite, rutile, and uraninite occur as accessory minerals in the equigranular facies. Secondary minerals are muscovite, sericite, kaolinite, and opaque minerals. Secondary uranophane occurs in the equigranular and border facies. In localized areas, the equigranular facies contains small, green idiomorphic crystals of beryl (Be3Al2Si6O18) as accessory mineral. One of these beryl crystals was chemically analyzed for major and minor element contents using an electron microprobe andthis information, along with fractional crystallization models and comparison with compositions of non-pegmatitic beryl from the literature, were used to understand the degree of evolution of the granitic melt. The chemical formula of beryl from the La Chinchilla Stock can be written as: C(Na0.014-0.033, K0.001-0.002, Ca0.001-0.004) T(2)(Be2.978-2.987, Li0.016-0.022) O(Al1.889-1.967, Fe0.045-0.103, Mg0.001-0.007, Mn0.001-0.007) T(1)(Si5.994-6.040O18). The alkali contents are low (Na2O6; FeOt<1.27 wt%). In a longitudinal geochemical profile, Al enrichment is observed at the border while the highest Na content is found in an internal point. In a transversal geochemical profile, the highest concentration of Al is seen in an internal point while Na remains almost invariable. Ferromagnesian elements vary randomly within the crystal. This indicates compositional changes in the magma for Al, ferromagnesian elements, and Na. The FeOt content of the analyzed beryl is within the compositional range of other disseminated beryl from granitoids but slightly higher than that of beryl from hydrothermal veins and greisens. It contains similar to slightly lower amounts of FeOt, MgO, and Na2O than beryl from medium to little evolved granitic pegmatites. Overall, the composition of beryl in theLa Chinchilla Stock is quite similar to that from medium to poorly evolved granitic pegmatites of the nearby Velasco Pegmatite District. The formation of beryl in the La Chinchilla Stock is attributed to precipitation from a F-bearing, highly fractionated, Al- and Si-rich melt saturated in BeO. A fractional crystallization model using Rb and Ba suggests that the beryl-hosting rock crystallized from the parent melt after extreme fractionation and 75% crystallization. Theoccurrence of beryl as a magmatic accessory mineral in the equigranular facies of the La Chinchilla Stock is indicative of a very high degree of fractionation of the parental magma.","palabras_clave":"NON-PEGMATITIC BERYL|CARBONIFEROUS GRANITE|LA CHINCHILLA STOCK|VELASCO RANGE|PAMPEAN PROVINCE|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6191,"idioma_id":4,"titulo":"Upper Limits from Five Years of Blazar Observations with the VERITAS Cherenkov Telescopes","resumen":"Between the beginning of its full-scale scientific operations in 2007 and 2012, the VERITAS Cherenkovtelescope array observed more than 130 blazars; of these, 26 were detected as very-high-energy (VHE; E>100GeV) &#947;-ray sources. In this work, we present the analysis results of a sample of 114 undetected objects. Theobservations constitute a total live-time of &#8764; 570 hours. The sample includes several unidentified Fermi-LargeArea Telescope (LAT) sources (located at high Galactic latitude) as well as all the sources from the secondFermi-LAT catalog which are contained within the field of view of the VERITAS observations. We have also performed optical spectroscopy measurements in order to estimate the redshift of some of these blazars that do not have a spectroscopic distance estimate. We present new optical spectra from the Kast instrument on the Shane telescope at the Lick observatory for 18 blazars included in this work, which allowed for the successful measurement or constraint on the redshift of four of them. For each of the blazars included in our sample we provide the flux upper limit in the VERITAS energy band. We also study the properties of the significance distributions and we present the result of a stacked analysis of the data-set, which shows a 4 &#963; excess.","palabras_clave":"BL Lacertae objects: general| galaxies: active|gamma rays: galaxies|radiation mechanisms: non-thermal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6192,"idioma_id":4,"titulo":"Numerical and experimental study of unsteady wind loads on panels of a radar aerial","resumen":"This work experimentallyand numerically analyzes the flow configurations and the dynamic wind loads onpanels of rectangular L\/h 5:1 cross section mounted on a structural frame ofrectangular bars of L\/h 0.5:1, corresponding to a radar structure. The fluid dynamicinteraction between panels and frame wakes imposes dynamic loads on the panels,with particular frequencies and Strouhal numbers, different from those ofisolated elements. The numerical scheme is validated by comparison with meanforces and velocity spectra of a panel wake obtained by wind tunnel tests. Theflow configuration is analyzed through images of the numerical simulations. Fora large number of panels, as in the radar array, their wakes couple in either phaseor counter-phase configurations, changing the resultant forces on each panel. Instantaneousnormal and tangential force coefficients are reported; their spectra show twodistinct peaks, caused by the interaction of the wakes. Finally, a scaled modelof a rectangular structure comprised of panels and frame elements is tested inthe boundary layer wind tunnel in order to determine the influence of thevelocity variation with height and the three-dimensionality of the bulk flow aroundthe structure. Results show that the unsteady aerodynamic loads, being stronglyinfluenced by the vortex shedding of the supporting elements and by the global3-D geometry of the array, differ considerably on a panel in this array fromloads acting on an isolated panel, not only in magnitude, but also in frequency.","palabras_clave":"WAKE ITERECTION|DYNAMIC LOADS|STROUHAL NUMBER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6193,"idioma_id":4,"titulo":"Field evaluation of oxygen consumption by two freshwater decapod morphotypes (Trichodactylidae and Aeglidae); the effect of different times of the day, body weight and sex","resumen":"We report an analysis of the effect of two different times of day (noonand dusk), body weight and sex on the oxygen consumption rate (OCR)of the freshwater crabs Trichodactylus kensleyi (Trichodactylidae), Aeglasingularis and A. platensis (Aeglidae) in their natural environment. Bothfamilies are sympatric in the studied locations, with a co-occurrencefound between T. kensleyi and A. singularis or T. kensleyi and A. platensis.The mean OCR was highest in A. singularis and lowest in A. platensis.The OCR was higher in Aegla species at noon, and Trichodactylusconsumed more oxygen at dusk. In aeglids, there was no differencein oxygen consumption between noon and dusk. T. kensleyi exhibitedstatistically significant differences in the OCR between noon and dusk.The oxygen uptake of all three species analysed was not influencedby the sex of the individual but varied according to the animals?weight. The families evaluated share some biological and ecologicalcharacteristics, such as diet and habitats, but the strategies used in theregulation of gas exchange at different times of the day were different.Environmental factors may be influencing the oxygen consumptionof each morphotype differently.","palabras_clave":"AEGLA|TRICHODACTYLUS|FRESHWATER CRUSTACEANS|CRABS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6194,"idioma_id":4,"titulo":"Identification of Novel Chemical Scaffolds Inhibiting Trypanothione Synthetase from Pathogenic Trypanosomatids","resumen":"The search for novel chemical entities targeting essential and parasite-specific pathways is considered a priority for neglected diseases such as trypanosomiasis and leishmaniasis. The thiol-dependent redox metabolism of trypanosomatids relies on bis-glutathionylspermidine [trypanothione, T(SH2)], a low molecular mass cosubstrate absent in the host. In pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Thus, TryS qualifies as an attractive drug target candidate.","palabras_clave":"TRYPANOSOMIASIS|TRYPANOTHIONE SYNTHASE|INHIBITORS|HTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6195,"idioma_id":4,"titulo":"Field-induced multiple order-by-disorder state selection in an antiferromagnetic honeycomb bilayer lattice","resumen":"In this paper we present a detailed study of the antiferromagnetic classical Heisenberg model on a bilayer honeycomb lattice in a highly frustrated regime in presence of a magnetic field. This study shows strong evidence of entropic order-by-disorder selection in different sectors of the magnetization curve. For antiferromagnetic couplings J1=Jx=Jp\/3, we find that at low temperatures there are two different regions in the magnetization curve selected by this mechanism with different number of soft and zero modes. These regions present broken Z2 symmetry and are separated by a not fully collinear classical plateau at M=1\/2. At higher temperatures, there is a crossover from the conventional paramagnet to a cooperative magnet. Finally, we also discuss the low temperature behavior of the system for a less frustrated region, J1=Jx < Jp\/3.","palabras_clave":"ORDER BY DISORDER|DIMERS|FRUSTRATION|MONTE CARLO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6196,"idioma_id":4,"titulo":"Metastable and scaling regimes of a one-dimensional Kawasaki dynamics","resumen":"We investigate the large-time scaling regimes arising from a variety of metastable structures in a chain of Ising spins with both first- and second-neighbor couplings while subject to a Kawasaki dynamics. Depending on the ratio and sign of these former, different dynamic exponents are suggested by finite-size scaling analyses of relaxation times. At low but nonzero-temperatures these are calculated via exact diagonalizations of the evolution operator in finite chains under several activation barriers. In the absence of metastability the dynamics is always diffusive.","palabras_clave":"METASTABILITY|SCALING|DYNAMIC EXPONENTS|NON-UNIVERSALITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6197,"idioma_id":4,"titulo":"Trypanosoma cruzi extracts elicit protective immune response against chemically induced colon and mammary cancers","resumen":"Trypanosoma cruzi, the protozoan parasite that causes Chagas´ disease, has anticancer effects mediated, at least in part, by parasite-derived products which inhibit growth of tumor cells. We investigated whether immunity to T. cruzi antigens could induce antitumor activity, using two rat models which reproduce human carcinogenesis: colon cancer induced by 1,2-dimethylhydrazine (DMH), and mammary cancer induced by N-nitroso-N-methylurea (NMU). We found that vaccination with T. cruzi epimastigote lysates strongly inhibits tumor development in both animal models. Rats immunized with T. cruzi antigens induce activation of both CD4(+) and CD8(+) T cells and splenocytes from these animals showed higher cytotoxic responses against tumors as compared to rats receiving adjuvant alone. Tumor-associated immune responses included increasing number of CD11b\/c(+) His48(-) MHC II(+) cells corresponding to macrophages and\/or dendritic cells, which exhibited augmented NADPH-oxidase activity. We also found that T. cruzi lysate vaccination developed antibodies specific for colon and mammary rat cancer cells, which were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) in vitro. Anti-T. cruzi antibodies cross-reacted with human colon and breast cancer cell lines and recognized 41\/60 (68%) colon cancer and 38\/63 (60%) breast cancer samples in a series of 123 human tumors. Our results suggest that T. cruzi antigens can evoke an integrated antitumor response involving both the cellular and humoral components of the immune response and provide novel insights into the understanding of the intricate relationship between parasite infection and tumor growth.","palabras_clave":"TRYPANOSOMA|IMMUNITY|CANCER|CROSS-REACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6198,"idioma_id":4,"titulo":"Automated and inexpensive method to manufacture solidstate nanopores and micropores in robust silicon wafers","resumen":"In this work an easy, reproducible and inexpensive technique for the production of solid state nanopores and micropores using silicon wafer substrate is proposed. The technique is based on control of pore formation, by neutralization etchant (KOH) with a strong acid (HCl). Thus, a local neutralization is produced around the nanopore, which stops the silicon etching. The etching process was performed with 7M KOH at 80°C, where 1.23µm\/min etching speed was obtained, similar to those published in literature. The control of the pore formation with the braking acid method was done using 12M HCl and different extreme conditions: i) at 25ºC, ii) at 80°C and iii) at 80°C applying an electric potential. In these studies, it was found that nanopores and micropores can be obtained automatically and at a low cost.Additionally, the process was optimized to obtain clean silicon wafers after the pore fabrication process. This method opens the possibility for an efficient scale-up from laboratory production.","palabras_clave":"NANOPORE|SILICON WAFER|METHOD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6199,"idioma_id":4,"titulo":"Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production","resumen":"Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we cultured HEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydimethylsiloxane (PDMS) microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysineimproves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of the microchannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.","palabras_clave":"LAB ON A CHIP|MICROFLUIDIC|MICROCHANNEL|CELL CULTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6200,"idioma_id":4,"titulo":"Terpenoid Derivatives as Potential Trypanocidal Agents","resumen":"In this review we refer to a promising family of molecules in the fight against Chagas disease. Terpene derivatives are abundant in the plant Kingdom and in the last years many compounds have shown important biological activities. Diterpenes and sesquiterpene lactones have shown in vitro and in vivo trypanocidal activity and they emerge as potential antichagasic drugs. These molecules may act on these parasites by multiple mechanisms, as it has been reported. Moreover, given the chemical structure of these compounds it is feasible to modify these molecules by chemical substitution in order to optimize their action against parasites","palabras_clave":"TRYPANOSOMA|CHAGAS|TRYPANOCIDAL|TERPENOID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6201,"idioma_id":4,"titulo":"Multigene characterisation of the lymnaeid vectors and climatic-environmental assessment of the transmission pattern","resumen":"Background: In South America, fascioliasis stands out due to the human endemic areas in many countries. In Argentina, human endemic areas have recently been detected. Lymnaeid vectors were studied in two human endemic localities of Catamarca province: Locality A beside Taton and Rio Grande villages; Locality B close to Recreo town.Methods: Lymnaeids were characterised by the complete sequences of rDNA ITS-2 and ITS-1 and fragments of the mtDNA 16S and cox1. Shell morphometry was studied with the aid of a computer image analysis system. Climate analyses were made by nearest neighbour interpolation from FAO data. Koeppen & Budyko climate classifications were used. De Martonne aridity index and Gorczynski continentality index were obtained. Lymnaeid distribution was assessed in environmental studies.Results: DNA sequences demonstrated the presence of Lymnaea neotropica and L. viator in Locality A and of L. neotropica in Locality B. Two and four new haplotypes were found in L. neotropica and L. viator, respectively. For interspecific differentiation, ITS-1 and 16S showed the highest and lowest resolution, respectively. For intraspecific analyses, cox1 was the best marker and ITS-1 the worst. Shell intraspecific variability overlapped in both species, except maximum length which was greater in L. viator. The desertic-arid conditions surrounding Locality A, thesemiaridity-aridity surrounding Locality B, and the very low yearly precipitation in both localities, are very different from the typical fascioliasis transmission foci. Lymnaeids are confined to lateral river side floodings and small man-made irrigation systems. Water availability only depends on the rivers flowing from neighbouring mountains. All disease transmission factors are concentrated in small areas where humans and animals go for water supply, vegetable cultures and livestock farming.Conclusions: The unusually high number of DNA haplotypes and the extreme climate unsuitable for F. hepática and lymnaeid development, demonstrate that the transmission foci are isolated. Seasonal transmission may depend on the timely overlap of appropriate temperature and river water availability. Lymnaeids and F. hepatica have probably reached these localities by livestock introduction. DNA differences regarding other populations of L. neotropica and L. viator in Argentina suggest an introduction independent from the spreading movements which allowed these two lymnaeids to expand throughout the country.","palabras_clave":"FASCIOLIASIS|HUMAN INFECTION|LYMNAEA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6202,"idioma_id":4,"titulo":"Digestive and metabolic profile at the biochemical level of  juvenile flounder Paralichthys orbignyanus(Valenciennes,1839) (Pleuronectiformes: Paralichthyidae)","resumen":"Abstract; The flounder Paralichthys orbignyanus that spans from Rio de Janeiro (Brazil) toSan Matías Gulf, Argentina has an important ecological role and a great potential use foraquaculture. However, studies on its digestive physiology (i.e. occurrence of digestiveenzymes) as well on energy reserves content in metabolic tissues are lacking. We determined theoccurrence and characteristics of amylase, maltase, sucrase, lipase and aminopeptidase-N indigestive tract of juveniles as an index of the ability to digest dietary glycogenic substrates fromdifferent sources, lipids and for final protein digestion. Glycogen, triglycerides and proteincontent in different tissues were also analyzed to identify storage sites. Presence and distributionin the digestive tract of the enzymes amylase, maltase, sucrase, lipase and N-aminopeptidase, aswell as glycogen, triglycerides and protein suggest that juveniles of P. orbignyanus exhibit anadequate digestive battery to potentially perform complete hydrolysis of various dietarysubstrates and capacity for storage and\/or utilization of energy reserves in liver and muscle.Moreover, our results show that juveniles of P. orbignyanus could accept a diet with highcarbohydrates content, which could represent and advantage for its potential application in fishfarming due to the possibility to use cheaper diets.Keywords: flatfishes, digestive enzymes, energy reserves","palabras_clave":"FLATFISHES|DIGESTIVE ENZYMES|ENERGY RESERVES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6203,"idioma_id":4,"titulo":"Lactobacillus delbrueckii subsp lactis CIDCA 133 modulates response of human epithelial and dendritic cells infected with Bacillus cereus","resumen":"Several studies demonstrate that fermented dairy products containing probiotic microorganisms promote human health. In the present work we focus on the effect of a potentially probiotic lactobacillus strain (Lactobacillus delbrueckii subsp. lactis CIDCA 133) in an in vitro model of Bacillus cereus infection. Our results showed that infection of HT-29 cells by B. cereus induces signaling through NF-&#954;B pathway and that the presence of strain CIDCA 133 increases stimulation. In contrast, B. cereus-induced IL-8 production is partially abrogated by strain CIDCA 133 thus suggesting multiple signaling pathways. Epithelial (HT-29 cells) and monocyte-derived dendritic cells (DC) were used in co-culture. After infection of HT-29 cells (upper compartment), B. cereus B10502 was found in the lower compartment interacting with dendritic cells (DC) although this interaction was diminished in the presence of strain CIDCA 133. Expression of HLA-DR and CD86 was induced in DC by infection B. cereus of HT-29 cells in the upper compartment and no further effect was observed in the presence of strain CIDCA 133. Analysis of supernatants of the lower compartment (DC) of co-cultures revealed the ability of strain CIDCA 133 to increase the production of TNF-&#945; triggered by B. cereus B10502 whereas no effect on the producton of IL-8 and IL-6 were found. The present work demonstrates for the first time the effect of a potentially probiotic strain (Lactobacillus delbrueckii subsp. lactis CIDCA 133) in an in vitro model of B. cereus infection. Furthermore the relevance of the cellular cross?talk both in the pathogenesis of B. cereus and in the possible effect of potentially probiotic strains is emphasized.","palabras_clave":"lactic acid bacteria|dendritic cells|bacilus cereus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6204,"idioma_id":4,"titulo":"Insights into the Andean genera Bridgesia and Guindilia (Sapindaceae): an integrated approach","resumen":"Sapindaceae s.l. is a moderately large family of trees, shrubs and lianas. The genera Bridgesia and Guindilia belong to the Thouinieae tribe; however, its circumscription to this tribe is in doubt. This work presents a comparative analysis of pollen morphology between species of both genera. They share the basic spherical tricolporate pollen type for the family, but the features studied do not characterize any of them. In addition, the work intends to characterize the karyotype and genome size evolution of both genera, and elucidate the phylogenetic relationships within the family through maximum likelihood and Bayesian analyses of the ITS, trnL and trnL-F sequences. Our results show two different patterns regarding karyotype data: Bridgesia presents 2n = 2x = 28, with metacentric and submetacentric chromosomes; the basic number of the genus, x = 14, is in agreement with the 25% of the chromosome numbers recorded in Sapindaceae. The genus Guindilia exhibits a basic number x = 10; of the three species G. cristata and G. trinervis present metacentric, submetacentric and subtelocentric chromosomes, whereas G. dissecta shows only metacentric and subtelocentric ones. In addition, G. cristata is a polyploid species, with exactly DNA content three-fold that of the diploid species, suggesting a recent event of polyploidization in this species. The infra-familial phylogenetic relationship and circumscription of both genera analysed here evidence that Bridgesia belongs to Paullinia group. The fact that Guindilia is grouped in a different clade encouraged us to propose a new informal tribal group, Guindilia group, in the current infrafamilial arrangement of Sapindaceae.","palabras_clave":"Bridgesia|Genome size|Guindilia|Karyotype|Phylogeny|Pollen morphology|Sapindaceae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6206,"idioma_id":4,"titulo":"Intestinal and sublingual microcirculation are more severely compromised in hemodilution than in hemorrhage","resumen":"The alterations in O2 extraction in hemodilution have been linked to fast red blood cell (RBC) velocity, which might affect the complete release of O2 from Hb. Fast RBC velocity might also explain the normal mucosal-arterial Pco2 (&#916;Pco2). Yet sublingual and intestinal microcirculation have not been completely characterized in extreme hemodilution. Our hypothesis was that the unchanged &#916;Pco2 in hemodilution depends on the preservation of villi microcirculation. For this purpose, pentobarbital-anesthetized and mechanically ventilated sheep were submitted to stepwise hemodilution (n = 8), hemorrhage (n = 8), or no intervention (sham, n = 8). In both hypoxic groups, equivalent reductions in O2 consumption (V&#775;o2) were targeted. Microcirculation was assessed by videomicroscopy, intestinal &#916;Pco2 by air tonometry, and V&#775;o2 by expired gases analysis. Although cardiac output and superior mesenteric flow increased in hemodilution, from the very first step (Hb = 5.0 g\/dl), villi functional vascular density and RBC velocity decreased (21.7 ± 0.9 vs. 15.9 ± 1.0 mm\/mm(2) and 1,033 ± 75 vs. 850 ± 79 &#956;m\/s, P < 0.01). In the last stage (Hb = 1.2 g\/dl), these variables were lower in hemodiution than in hemorrhage (11.1 ± 0.5 vs. 15.4 ± 0.9 mm\/mm(2) and 544 ± 26 vs. 686 ± 70 &#956;m\/s, P < 0.01), and were associated with lower intestinal fractional O2 extraction (0.61 ± 0.04 vs. 0.79 ± 0.02, P < 0.01) but preserved &#916;Pco2 (5 ± 2 vs. 25 ± 4 mmHg, P < 0.01). Therefore, alterations in O2 extraction in hemodilution seemed related to microvascular shunting, not to fast RBC velocity. The severe microvascular abnormalities suggest that normal &#916;Pco2 was not dependent on CO2 washout by the villi microcirculation. Increased perfusion in deeper intestinal layers might be an alternative explanation.","palabras_clave":"HEMORRHAGIC|SHOCK|MICROCIRCULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6207,"idioma_id":4,"titulo":"Post-anthesis warm nights reduce grain weight in field-grown wheat and barley","resumen":"Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) crops are exposed to warm nights during their growing seasons and this trend is unlikely to change. The aim of this work was to evaluate the effect of higher post-anthesis night temperatures on field-grown crop yield, focusing on final grain weight determination. Experiments combined: (i) two well-adapted crops with similar phenology: bread wheat and two-row malting barley, under (ii) two temperature regimes: ambient and high night temperatures from 10 days after anthesis to physiological maturity during (iii) two contrasting growing seasons in terms of radiation and temperature: late sowing in 2011 and early sowing in 2013. The night temperature increase (ca. 4.1 °C) was achieved using purpose-built heating chambers placed on the crop at 7 pm and removed at 7 am every day during the heating period. Across growing seasons and crops, the average minimum temperature during that period ranged from 14.3 °C to 21.9 °C. Thousand grain weight was reduced by ca. 3% per °C of night temperature increase, similarly for wheat and barley, causing a grain yield reduction of ca. 4% per °C. An accelerated development under high night temperatures led to a shorter effective grain filling period, reducing the final grain weight. The lack of consistent impact on source availability between crops and seasons, measured as senescence and stem water soluble carbohydrates, as well as a similar impact in magnitude and direction on individual grain weight for different grain positions along wheat or barley spikes, suggest that the negative effects of warm nights on grain weight were directly related to processes within the grain itself.","palabras_clave":"Climate change|Grain filling duration|Grain weight|Minimum temperature|Temperate cereals"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6209,"idioma_id":4,"titulo":"Dipolar interactions between domains in lipid monolayers at the air-water interface","resumen":"A great variety of biologically relevant monolayers present phase coexistence characterized by domains formed by lipids in an ordered phase state dispersed in a continuous, disordered phase. The difference in surface densities between these phases originates inter?domain dipolar interactions, which are relevant for the determination of the spacial distribution of domains, as well as their dynamics. In this work, we propose a novel manner of estimating the dipolar repulsion using a passive method that involves the analysis of images of the monolayer with phase coexistence. The method is based on the comparison of the pair correlation function obtained from experiments with that obtained from Brownian dynamics simulations of a model system. As an example, we determined the difference in dipolar density of a binary monolayer of DSPC\/DMPC at the air?water interface from the analysis of the radial distribution of domains, and the results are compared with those obtained by surface potential determinations. A systematic analysis for experimentally relevant parameter range is given, which may be used as a working curve for obtaining the dipolar repulsion in different systems.","palabras_clave":"DIPOLAR REPULSION|MODULATED PHASES|DIPOLAR DENSITY IN LIPID MONOLAYERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6210,"idioma_id":4,"titulo":"Image enhancement by spatial frequency post-processing of images obtained with pupil filters","resumen":"The useofapodizingorsuperresolving filters improvestheperformanceofanopticalsystemindifferentfrequencybands.ThisimprovementcanbeseenasanincreaseintheOTFvaluecomparedtotheOTFforthe clearaperture.In thispaperweproposeamethodtoenhancethecontrastofanimageinbothitslowanditshighfrequencies.ThemethodisbasedonthegenerationofasyntheticOpticalTransferFunction,bymulti-plexingtheOTFsgivenbytheuseofdifferentnon-uniformtransmission filters onthepupil.Weproposeto capturethreeimages,oneobtainedwithaclearpupil,oneobtainedwithanapodizing filter thatenhances thelowfrequenciesandanotheronetakenwithasuperresolving filter thatimprovesthehighfrequencies.IntheFourierdomainthethreespectraarecombinedbyusingsmoothedpassband filters,and thentheinversetransformisperformed.Weshowthatwecancreateanenhancedimagebetterthanthe imageobtainedwiththeclearaperture.Toevaluatetheperformanceofthemethod,bartests(si-nusoidal tests)withdifferentfrequencycontentareused.Theresultsshowthatacontrastimprovementin thehighandlowfrequenciesisobtained.","palabras_clave":"IMAGE PROCESSING|PUPIL FILTERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6211,"idioma_id":5,"titulo":"Diversity of copepods in Atlantic patagonian coastal waters throughout an annual cycle","resumen":"The aim of this study is to analyze the annual zooplankton succession at a coastal station in Patagonia (Argentina), with special emphasis on copepods and their potential preys, using solar radiation and temperature as environmental factors. The annual plankton cycle exhibited autumn\/winter and spring\/summer phases. During the autumn\/winter phase, low temperature and radiation were correlated with higher diatom abundance and copepod diversity. This period was characterized by a dominant classical herbivorous food web in which the large copepod Calanoides carinatus dominated the community. On the contrary, during the spring\/summer period, with high radiation and temperature levels, flagellates were the dominant food available for zooplankton and correlated with smaller species of copepods such as the harpacticoid Euterpina acutifrons. Also, intensive reproduction of the small copepods E. acutifrons and Paracalanus parvus occurred mainly in summer. On the other hand, high species diversity was found in winter when the ultraviolet radiation was low. The temporal pattern of the copepods observed in our study was explained by food availability and environmental factors (temperature and solar radiation), although other factors such as predator abundance may also be important in modulating the community. Our results show the importance of copepod abundance dynamics and highlights their key role in the pelagic food web in northern Patagonian coastal waters.","palabras_clave":"ZOOPLANCTON|CICLO ESTACIONAL|ESTUARIO|MAR ARGENTINO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6212,"idioma_id":4,"titulo":"Travel for sex: long range breeding dispersal and winter haulout fidelity in Southern Sea Lion males","resumen":"Southern sea lions (Otaria flavescens; SSL) have a polygynous mating system and a prolonged social-sexual maturation period for males. Male haulouts are common in this species, with some very distant from central breeding rookeries, although the functions of these aggregations are not clearly understood. To estimate the potential connectivity between northern Argentina male colonies and breeding rookeries in Patagonia and Uruguay, we monitored the summer breeding activities and winter presence of 559 individually identified SSL males from haulouts in Mar del Plata and Quequén harbors. Our results confirm that male groups are formed by sexually active sea lions that show a strong annual connection with distant (up to 700 km) breeding colonies. Circa 70% of the marked males made long distance round-trips (72 ± 26.7 days; n = 325) from northern Argentina to Patagonia and Uruguay, indicating a high degree of winter site fidelity. Mating activity was confirmed for 53% of the sea lions re-sighted in breeding colonies, with ca 80% of them having central positions on the beach and holding harems of up to nine females. The chronology of this cycle is finely tuned with the onset of the breeding season which may result in comparative advantages such as anticipating female arrival or a prolonged participation in mating activities. Our results suggest a model of male haulouts spatially segregated from the central breeding areas, but with a summer recurrent flow of animals that contributes a significant proportion of the male population of northern Patagonia and Uruguay","palabras_clave":"Otaria flavescens|Pinnipeds|Sea Lions|Connectivity|Mating System|Male hauling grounds|Argentina|Marking|Spatial segregation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6213,"idioma_id":4,"titulo":"Intermediate magnetisation state, distortions and competing orders in DyTiO and HoTiO7","resumen":"Among the frustrated magnetic materials, spin-icestands out as a particularly interesting system. Residual entropy, freezing and glassiness, Kasteleyn transitions and fractionalisation of excitations in three dimensions allstem from a simple classical Hamiltonian. But is the usual spin-ice Hamiltonian a correct description of the experimental systems? Here we address this issue by measuring magnetic susceptibility in the two most studied spin-ice compounds, DyTiO and HoTiO, using a vector magnet. Using these results, and guided by a theoretical analysis of possible distortions to the pyrochlore lattice,we construct an effective Hamiltonian and explore it using Monte Carlo simulations. We show how this Hamiltonian reproduces the experimental results, including the formation of a phase of intermediate polarisation, and gives important information about the possible groundstate of real spin-ice systems. Our work suggests an unusual situation in which distortions might contribute to the preservation rather than relief of the effects of frustration.","palabras_clave":"FRUSTRATED SYSTEMS|MAGNETISM|SPIN-ICE|DISTORTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6214,"idioma_id":4,"titulo":"CFTR channel in oocytes from Xenopus laevis and its regulation by xShroom1 protein","resumen":"Shroom is a family of related proteins linked to the actin cytoskeleton. xShroom1 is constitutively expressed in Xenopus laevis oocytes, and it is required for the expression of the epithelial sodium channel (ENaC). As there is a close relationship between ENaC and the cystic fibrosis transmembrane regulator (CFTR), we examined the action of xShroom1 on CFTR expression and activity. Biotinylation was used to measure CFTR surface expression, and currents were registered with voltage clamp when stimulated with forskolin and 3-isobutyl-1-methylxanthine. Oocytes were coinjected with CFTR complementary RNAs (cRNAs) and xShroom1 sense or antisense oligonucleotides. We observed an increment in CFTR currents and CFTR surface expression in oocytes coinjected with CFTR and xShroom1 antisense oligonucleotides. MG-132, a proteasome inhibitor, did not prevent the increment in currents when xShroom1 was suppressed by antisense oligonucleotides. In addition, we inhibited the delivery of newly synthesized proteins to the plasma membrane with BFA and we found that the half-life of plasma membrane CFTR was prolonged when coinjected with the xShroom1 antisense oligonucleotides. Chloroquine, an inhibitor of the late endosome\/lysosome, did not significantly increase CFTR currents when xShroom1 expression was inhibited. The higher expression of CFTR when xShroom1 is suppressed is in concordance with the functional studies suggesting that the suppression of the xShroom1 protein resulted in an increment in CFTR currents by promoting the increase of the half-life of CFTR in the plasma membrane. The role of xShroom1 in regulating CFTR expression could be relevant in the understanding of the channel malfunction in several diseases.","palabras_clave":"CFTR|SHROOM|OOCYTES|IONCURRENTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6215,"idioma_id":4,"titulo":"Lyapunov decay in quantum  irreversibility","resumen":"The Loschmidt echo?also known as fidelity?is a very useful tool to study irreversibility in quantum mechanics due to perturbations or imperfections. Many different regimes, as a function of time and strength of the perturbation, have been identified. For chaotic systems, there is a range of perturbation strengths where the decay of the Loschmidt echo is perturbation independent, and given by the classical Lyapunov exponent. But observation of the Lyapunov decay depends strongly on the type of initial state upon which an average is carried out. This dependence can be removed by averaging the fidelity over the Haar measure, and the Lyapunov regime is recovered, as has been shown for quantum maps. In this work, we introduce an analogous quantity for systems with infinite dimensional Hilbert space, in particular the quantum stadium billiard, and we show clearly the universality of the Lyapunov regime.","palabras_clave":"irreversibility|Lyapunov decay|Loschmidt echo|Quantum chaos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6216,"idioma_id":4,"titulo":"The H I supershell GS 118+01-44 and its role in the interstellar medium","resumen":"Aims: We carry out a multiwavelength study to characterize the HI supershell designated GS 118+01-44, and to analyse its possibleorigin.  Methods: A multiwavelength study has been carried out tostudy the supershell and its environs. We performed an analysis of the HI, CO, radio continuum, and infrared emission distributions. Results: The Canadian Galactic Plane Survey (CGPS) H I data reveals thatGS 118+01-44 is centred at (l,b) = (117°&amp;dotbelow;7,1°&amp;dotbelow;4) with a systemic velocity of -44.3 km s-1.According to Galactic rotation models this structure is located at 3.0± 0.6 kpc from the Sun. There are several H II regions and threesupernova remnants (SNRs) catalogued in the region. On the other hand,the analysis of the temperature spectral index distribution shows thatin the region there is a predominance of non-thermal emission. Infraredemission shows that cool temperatures dominate the area of thesupershell. Concerning the origin of the structure, we found that eventhough several OB stars belonging to Cas OB5 are located in the interiorof GS 118+01-44, an analysis of the energy injected by these starsthrough their stellar winds indicates that they do not have sufficientenergy to create GS 118+01-44. Therefore, an additional energy source isneeded to explain the genesis of GS 118+01-44. On the other hand, thepresence of several H II regions and young stellar object candidates inthe edges of GS 118+01-44 shows that the region is still active informing new stars.","palabras_clave":"ISM: bubbles|ISM: kinematics and dynamics|HII regions|stars: formation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6217,"idioma_id":4,"titulo":"Effect of Sanitizers and Glucose on Clostridium perfringens  Biofilm Formation and Growth","resumen":"Clostridium perfringens enterotoxigenic strain is one of the responsible of food poisoning; this is caused by cpe enterotoxin. Cpe is produced during sporulation, a resistance mechanism. It is described that C. perfringens can form biofilm under stressed environments and this depends of carbohydrates availability. The aim of this work was to evaluate the ability of enteroxigenic (E+) and no enterotoxigenic (E-) C. perfringens strains to form biofilm at different concentrations of carbon source and the inhibitory effect of various Poli P over biofilm formation and the expression of cpe and ccpa genes. Our results indicate that E+ C. perfringens biofilm reach the more complex structure with high glucose concentrations. Even more current sanitizers used in food industry like SAPP and TSPP, showed to stimulate biofilm formation and to inhibit the growth of planktonic cells. On the other hand, morphological changes were observed during biofilm formation under low glucose concentration and in presence of sanitizers. We propose that the change from a bacillary to coccoid form is related to a resistance to stressed environments. Finally the cpe expression is not related to biofilm formation.","palabras_clave":"Biofilm|C. perfringens|sanitizer|glucose|morphology|CcpA|cpe"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6218,"idioma_id":5,"titulo":"ARTESANOS EMPRENDEDORES DE LA ARTESANÍA LOCAL EN LA PROVINCIA DE GRANMA, CUBA","resumen":"La provincia Granma se encuentra ubicada en la región sur-oriental de Cuba. Su riqueza natural constituye uno de sus principales atractivos, al mismo tiempo que proporciona la materia prima fundamental para desarrollar una de las expresiones culturales que más fuerzas ha adquirido en los últimos años, la artesanía. El uso racional de productos naturales constituye actualmente el soporte básico para la creación y desarrollo de objetos artesanales, los cuales son representativos de la cubanía e identidad nacional, así como portadores de utilidad práctica y de valores decorativos. Las industrias culturales se clasifican en nueve grupos según el Observatorio de Cultura y Economía (2011), en la cual se reconoce la artesanía, por lo tanto es un motor impulsor de la cultura y de las tradiciones. El estudio del fenómeno del emprendimiento se considera necesario para obtener una obra auténtica, por lo tanto en este artículo se abordan algunos aspectos que se consideran relevantes en torno al emprendimiento. El presente trabajo tiene como objetivo general analizar el comportamiento de la artesanía y el fenómeno del emprendimiento en la provincia Granma, como elemento dinamizador de la industria cultural. Se analiza una muestra de artesanos tomados al azar a través del método estadístico Análisis de Correspondencia Simple, el cual arrojó resultados muy interesantes relacionados con las variables capacitación, motivación e innovación. Finalmente se propone un plan de acciones para fomentar el desarrollo artesanal en la provincia Granma.","palabras_clave":"ARTESANIA|EMPRENDEDORES|IDENTIDAD|DESARROLLO LOCAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6219,"idioma_id":4,"titulo":"The first Parthenogenetic Pleurodont Iguanian: A new All-female Liolaemus (Squamata: Liolaemidae) from Western Argentina","resumen":"Vertebrate taxa with entirely unisexual populations are composed of females that reproduce without fertilization and are generally of hybrid origin. At least 23 vertebrate families have unisexual lineages. Within squamate reptiles, >40 uniparental species have been described from five major lineages: acrodont iguanians, Gekkota, Serpentes, Scincomorpha, and Varanidae. Pleurodont iguanians include >1100 species, all of which have gonochoristic reproduction. Here we report the discovery of a new unisexual lizard from the liolaemid genus Liolaemus, a species-rich (~260 species) clade distributed over most of southern South American. The new species, Liolaemus parthenos, is the only known all-female triploid pleurodont iguanian. The structural chromosome heteromorphisms of the karyotypes support the hypothesis that L. parthenos is the product of a hybrid origin. Based on morphology, the new species is assignable to the L. boulengeri group (sensu Abdala, 2007) and phylogenetic analyses of mitochondrial DNA indicate that L. parthenos is nested within L. darwinii, suggesting that the latter is the maternal ancestor of this species. Additional data are needed to identify the patrilineal ancestor.","palabras_clave":"LIZARD|LIOLAEMUS|PARTHENOGENETIC|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6220,"idioma_id":4,"titulo":"Synthesis of organotin derivatives of optically active eleven-membered macrodiolides","resumen":"The synthesis and the results obtained in the hydrostannation of eight new TADDOL diacrylates and methacrylates are reported. The addition of triorganotin hydrides, R3SnH, 12?14 (R = nBu, neophyl, Ph,respectively) to diesters 6?11 containing different combinations of substituents at the C-2 carbon of the dioxolane ring, led to macrocyclization products in all cases. The cyclohydrostannation of diacrylate 10 proceeded with complete diastereoselectivity. The cyclohydrostannation of diesters 33 and 34 withhydrides 12 and 14 in all cases only afforded one stannylated macrocycle.","palabras_clave":"MACRODIOLIDES|SYNTHESIS|ORGANOTIN DERIVATIVES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6221,"idioma_id":4,"titulo":"Mechanical Behavior of Loose Sand Reinforced with Synthetic Fibers","resumen":"This work describes an experimental study about the effects the addition of randomly oriented polypropylene fibers have on the behavior of an alluvial fine to medium sand in a loose state. The study is focused on the characterization of the influence the fiber addition has on the shear strength and on the deformational modulus corresponding to low, medium, and large strain levels of the reinforced sand. The effect of the type (smooth and meshed), length, and fiber content, is analyzed by means of drained triaxial, direct shear, and shear wave velocity tests.","palabras_clave":"MECHANICAL BEHAVIOR|MEDIUM SAND|FIBERS|SHEAR STRENGTH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6222,"idioma_id":4,"titulo":"Xanthomonas campestris attenuates virulence by sensing light through a bacteriophytochrome photoreceptor","resumen":"Phytochromes constitute a major photoreceptor family found in plants, algae, fungi, and prokaryotes, including pathogens. Here we report that Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease which affects cruciferous crops worldwide, codes for a functional bacteriophytochrome (XccBphP). XccBphP possesses an N-terminal PAS2-GAF-PHY photosensory domain triad and a C-terminal PAS9 domain as its output module. Our findings show that illumination of Xcc, prior to plant infection, attenuates its virulence in an XccBphP-dependent manner. Moreover, in response to light, XccBphP down-regulates xanthan exopolysaccharide production and biofilm formation, two known Xcc virulence factors. Furthermore, the XccbphP null-mutant shows enhanced virulence, similar to that of dark-adapted Xcc cultures. Stomatal aperture regulation and callose deposition, both well-established plant defense mechanisms against bacterial pathogens, are overridden by the XccbphP strain. Additionally, an RNA-Seq analysis reveals that far-red light or XccBphP overexpression produce genome-wide transcriptional changes, including the inhibition of several Xcc virulence systems. Our findings show that Xcc senses light through XccBphP, eliciting bacterial virulence attenuation via down-regulation of bacterial virulence factors. The capacity of XccBphP to respond to light both in vitro and in vivo was abolished by a mutation on the conserved Cys13 residue. These results provide evidence for a novel bacteriophytochrome function affecting an infectious process.","palabras_clave":"phytochromes|xanthomonas|light|virulence"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6223,"idioma_id":4,"titulo":"DFT study of Li+ and Na+ positions in mordenites and hydration stability","resumen":"Mordenite is a type of zeolite used in catalysis processes and in the selective adsorption of gases. The properties of this material are related to the presence of aluminium atoms and exchangeable cations in the structure. In this work, we performed DFT periodic calculations of cationic mordenites X8Al8Si40O96 and X4Al4Si44O96 (X = Li+ or Na+) and the corresponding hydrated species. Calculations of Li+ and Na+ mordenites indicated preference for the aluminium atoms to occupy T3 and T4 sites. In this configuration,the alkaline cations have preference for the two channels 8MRz and 12MRz along [001] crystallographic axis. In the hydrated structures, the alkaline cations have preference for the side pockets (8MRy along [010] direction). Hydrated structures with high aluminium content have a stability caused by interaction of the alkaline cations with water molecules and oxygen atoms of the framework.","palabras_clave":"PERIODIC CALCULATIONS|MORDENITE|EXCHANGEABLE CATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6224,"idioma_id":4,"titulo":"A MAC Protocol to Support Monitoring of Underwater Spaces","resumen":"Underwater sensor networks are becoming an important field of research, because of their everyday increasing application scope. Examples of their application areas are environmental and pollution monitoring (mainly oil spills), oceanographic data collection, support for submarine geolocalization, ocean sampling and early tsunamis alert. The challenge of performing underwater communications is well known, provided that radio signals are useless in this medium, and a wired solution is too expensive. Therefore, the sensors in these networks transmit their information using acoustic signals that propagate well under water. This data transmission type not only brings an opportunity, but also several challenges to the implementation of these networks, e.g., in terms of energy consumption, data transmission and signal interference. In order to help advance the knowledge in the design and implementation of these networks for monitoring underwater spaces, this paper proposes a MAC protocol for acoustic communications between the nodes, based on a self-organized time division multiple access mechanism. The proposal was evaluated using simulations of a real monitoring scenario, and the obtained results are highly encouraging.","palabras_clave":"UNDERWATER SENSORS|UNDERWATER MONITORING|MAC PROTOCOL|ACOUSTIC TRANSMISSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6225,"idioma_id":4,"titulo":"Pharmacokinetic and pharmacodynamic responses in adult patients with Chagas disease treated with a new formulation of benznidazole","resumen":"Pharmacological treatment of Chagas disease with benznidazole (BNZ) is effective in children in all stages, but it is controversial in chronically infectedadults. We report the pharmacokinetics and pharmacodynamics in six adult patientswith Chagas disease treated with the new BNZ formulation (ABARAX®) in dosesbetween 2.5-5.5 mg\/Kg\/day. All but one patient had plasmatic BNZ concentrationswithin the expected range. All patients finalised treatment with nondetectableTrypanosoma cruzi quantitative polymerase chain reaction, which remainednondetectable at the six month follow-up. Our data suggests parasitologicalresponses with the new BNZ and supports the hypothesis that treatment protocolswith lower BNZ doses may be effective","palabras_clave":"pharmacology|Chagas disease|benznidazole"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6226,"idioma_id":4,"titulo":"A time-series analysis of therapeutic alliance, interventions, and client?s clinical status in an evidence-based single-case study: Evidence for establishing change mechanisms in psychotherapy","resumen":"Objective: The goal of this study was to analyze the time-series of alliance, interventions, and client?s post-sessions clinical status, to establish if alliance and adherence to cognitive-behavioral interventions preceded improvement in psychotherapy Method: A single-case study of a complete Cognitive-Behavioral treatment of a 27-year-old male diagnosed with Generalized Anxiety Disorder treatment was conducted. Alliance, adherence to cognitive-behavioral interventions, and client?s therapeutic condition were assessed every two sessions during the entire treatment. Results: After controlling for the effect of autocorrelations, the transfer functions showed that alliance predicted client?s clinical condition with a lag of two sessions throughout the entire treatment. However, the inverse relationship was not observed. Conclusions: Results support the hypothesis of a time-lagged association between alliance and subsequent client?s changes in their clinical condition in single case of a cognitive-behavioral treatment.","palabras_clave":"therapeutic alliance|interventions|mechanism of change|change"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6227,"idioma_id":4,"titulo":"Neutrino emissivity in the quark-hadron mixed phase of neutron stars","resumen":"Numerous theoretical studies using various equation of state models have shown that quark matter may exist at the extreme densities in the cores of high-mass neutron stars. It has also been shownthat a phase transition from hadronic matter to quark matter would result in an extended mixed phase region that would segregate phases by net charge to minimize the total energy of the phase, leading to the formation of a crystalline lattice. The existence of quark matter in the core of a neutron star may have significant consequences for its thermal evolution, which for thousands of years is facilitated primarily by neutrino emission. In this work we investigate the effect a crystalline quark-hadron mixed phase can have on the neutrino emissivity from the core. To this end we calculate the equation of state using the relativistic mean-field approximation to model hadronic matter and a nonlocal extension of the three flavor Nambu?Jona-Lasinio model for quark matter. Next we determine the extent of the quark-hadron mixed phase and its crystalline structure using the Glendenning construction, allowing for the formation of spherical blob, rod, and slab rare phase geometries. Finally we calculate the neutrino emissivity due to electron-lattice interactions utilizing the formalism developed for the analogous process in neutron star crusts. We find that the contribution to the neutrino emissivity due to the presence of a crystalline quarkhadron mixed phase is substantial compared to other mechanisms at fairly low temperatures ( 109 K) and quark fractions ( 30%), and that contributions due to lattice vibrations are insignificant compared to static-lattice contributions.","palabras_clave":"Quark Matter |Equation of State|Neutron Stars"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6228,"idioma_id":5,"titulo":"¿Hacia dónde va América Latina? El desarrollo desigual en la periferia globalizada","resumen":"La hegemonía neoliberal a fines del siglo pasado opacó enfoques teóricos originales latinoamericanos, como el estructuralismo y la escuela de la dependencia. Tanto el ideario neoliberal como algunas perspectivas críticas ?posdependentistas? han postulado que las diferencias nacionales tienden a perder relevancia en el marco de la globalización. En este artículo se sostiene otra hipótesis: que en lugar de una convergencia entre centro y periferia, se está produciendo una creciente bifurcación de la periferia misma. Para ponerla a prueba se compara la evolución de dos regiones: el este de Asia y América Latina.","palabras_clave":"DESARROLLO DESIGUAL|PERIFERIA|GLOBALIZACIÓN|DEPENDENCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6230,"idioma_id":4,"titulo":"Puma density, habitat use and conflict with humans in the Argentine Chaco","resumen":"The puma Puma concolor is the most widely distributed felid in the Americas. Although it utilizes human-modified landscapes, its extensive territorial requirements, trophic needs, and real or perceived threats to livestock render the puma susceptible to conflict with humans. Our objectives were to evaluate the population density, habitat use, and puma-human conflict in the Argentine Chaco. We conducted camera-trap surveys and interviews over a three-year period, at three sites with different levels of legal protection and with different ranch outpost and livestock densities: Copo National Park (1,204 trap days, 24 stations, 17 interviews, national park, lowest ranch \/ livestock density), Aborigen Reserve (1,993 trap days, 30 stations, 13 interviews, indigenous reserve, medium ranch \/ livestock density) and El Cantor (2,129 trap days, 35 stations, 11 interviews, no protection, highest ranch \/ livestock density). Puma population density was low (<1 individual\/100 km2) and we found no significant differences in puma density across the three sites. Occupancy models show a positive relationship between puma detectability and the distances from vehicle roads. Legal protection status of the area does not positively affect puma density, probably due to the large edge effect, and weak law enforcement capacity at Copo National Park. Low density of pumas at the three sites could result primarily from retaliation killing of pumas by local ranchers in response to predation on goats. Pumas in the Chaco require effectively managed protected areas, regulation of wildlife hunting and livestock management practices to minimize depredation.","palabras_clave":"CAMERA TRAP|HUNTING|LIVESTOCK|OCCUPANCY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6231,"idioma_id":4,"titulo":"Local and continental determinants of giant anteater (Myrmecophaga tridactyla) abundance:  biome, human and jaguar roles in population regulation","resumen":"The giant anteater (Myrmecophaga tridactyla) is currently found in a wide variety of habitats from Honduras to Argentina. Across this wide range, researchers have postulated that anteater populations are negatively affected by several factors, including hunting, habitat loss and fragmentation, fire, vehicle collisions, and predation by jaguars. But no studies to date have evaluated the relative importance of these factors across sites, either at a regional or continental scale. We used camera traps to analyze variation in giant anteater abundance at two spatial scales. At a regional scale, we conducted camera trap surveys in the dry Chaco of Argentina and used occupancy models to explore the effect of protection status and human accessibility on giant anteaters? relative abundance. At a continental scale, we used data from 40 camera trap studies (representing 42 different locations) and Generalized Linear Models (GLM) to assess the potential relation of biome, human disturbance and the presence of jaguars on giant anteater camera trap records. In the Argentine Chaco, protection and human disturbance do not significantly affect the proportion of area used by the species. The average anteater records\/100 camera days and the proportion of sites used are very high across the study area. At a continental scale, anteaters are more frequently recorded in dry forests than in moist forests. Locations with very high human disturbance have camera trap rates that are 5-10 times lower compared to intermediate or low disturbance locations. Finally, giant anteater capture frequency increases up to 70% where jaguars are absent. Dry biomes and intermediate levels of human disturbance may favor anteaters by providing greater habitat heterogeneity coupled with a lower jaguar abundance. This may explain the relatively high abundance of giant anteaters in the Argentine Chaco. However, growing human populations, the advancing agriculture-livestock frontier, and an expanding road network may in time eliminate giant anteaters from most of this region.","palabras_clave":"Camera traps|Chaco|Conservation|Panthera onca"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6232,"idioma_id":5,"titulo":"Evaluación de la actividad inhibitoria del aceite esencial de Cymbopogon citratus y  cinco diluyentes sobre cultivos de Ascosphaera apis y su toxicidad en abejas adultas","resumen":"Se evalúo la concentración inhibitoria mínima (CIM) del aceite esencial de Cymbopogon citratus y cinco diluyentes sobre cultivos de Ascosphaera apis. Se determinó la toxicidad en abejas adultas y se calculó el radio de selectividad de la formulación aceite esencial-diluyente. Se determinó la CIM por difusión en agar MY20 de 28 aislados de A. apis de Argentina y Chile con los diluyentes Tween 80, dimetilsufóxido (DMSO), propilenglicol (PG), alcohol 70º y agua en concentraciones 150, 250, 500 y 750 mg\/L, formuladas en 2,5 % del diluyente v\/v. La toxicidad oral aguda sobre la abeja melífera adulta se evaluó para la combinación de C. citratus con PG y con alcohol 70°, usando dimetoato como control tóxico. El alcohol 70° resultó ser el mejor diluyente para el C. citratus  ya que presentó una CIM90 de 250 mg\/L, fue ?virtualmente no tóxico? para abejas adultas y presentó un radio de selectividad de 6 &#956;L\/abeja a las 72 horas transformándolo en la formulación aceite esencial?diluyente menos tóxica para el control de A. apis en colonias de abejas.","palabras_clave":"Ascosphaera apis|Apis mellifera|Cymbopogon citratus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6233,"idioma_id":4,"titulo":"Cerium, manganese and cerium\/manganese ceramic monolithic catalysts. Study of VOCs and PM removal","resumen":"Ceramic supported cerium, manganese and cerium-manganese catalysts were prepared by direct impregnation of aqueous precursor, and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Brunauer-Emmett-Teller method (BET), temperature programmed reduction (H2-TPR), X-ray photoelectron spectroscopy (XPS) acidity measurements and electrical conductivity. The catalytic activity was evaluated for volatile organic compounds (VOC) (ethanol, methyl ethyl ketone and toluene) oxidation. Additionally, catalysts were tested in particulate matter (PM) combustion. The characterization results indicated that Ce was in the form of Ce4+ and Ce3+, and Mn existed in the form of Mn4+ and Mn3+ on the surface of the Mn\/AC sample and in the form of Mn4+ in the Ce\/Mn\/AC monolith. VOC oxidation results revealed that the Ce\/Mn\/AC sample showed an excellent performance compared with ceramic supported CeO2 (Ce\/AC) and MnOx (Mn\/AC) samples. The PM combustion was also higher on Ce\/Mn\/AC monoliths. The enhanced catalytic activity was mainly attributed to the Ce and Mn interaction which enhanced the acidity, conductivity and the reducibility of the oxides.","palabras_clave":"MONOLITHS|MnOx|Ce|PM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6234,"idioma_id":4,"titulo":"Evasion and immuno-endocrine regulation in parasite infection: Two sides of the same coin in Chagas disease?","resumen":"Chagasdisease, also known as American trypanosomiasis, is a serious illness caused bythe protozoan parasite Trypanosoma cruzi(T. cruzi). Seven million people are infected worldwide. Although most ofthose infected are in Latin America, migration flows have spread the disease toother non-endemic continents. Chagas disease present an acute and a chronicphase, and both could be asymptomatic or symptomatic. While oral acute Chagasdisease has emerged nowadays as the principal acute and lethal endemic form of infection,almost 30% of chronically infected people (by vectorial, congenital,oral and otherforms) develop cardiac, digestive or mixed alterations, suggesting a broadrange of host-parasite interactions that finally impact upon chronic diseaseoutcome. The ability of T. cruzi topersist and cause pathology seems to depend on diverse factors likeT. cruzi strains and the infective load,the route of infection, the release of immunomodulatory or virulence factors,the parasite capacity of avoid protective immune response, the strength andtype of host defence mechanisms and the genetic background of the host. In thiscontext, the host-parasite interaction is subject to a constant neuro-endocrineregulation, and as the infection proceeds can lead to a broad range ofoutcomes, from elimination of the pathogen to its continued persistence in thehost with the consequent induction of pathology. In particular,&nbsp;neuro-endocrinebalance, effector and regulatory T cells are key points where the host can takeadvantage to improve their defence mechanisms, or contrarily may be exploitedby parasites to survive and persist. In this context, T. cruzi evasion strategies and hostdefence mechanisms can be envisioned as two sides from the same coin at thecomplex host-parasite relationship influencing parasite persistence and differentoutcomes observed in Chagas disease. Understanding on how T. cruzi evade innate and adaptive immune response and how parasitepersistence might be favoured by immuno-neuro-endocrine regulation will provideimportant clues to better dissect mechanisms underlying the pathophysiology ofChagas disease.","palabras_clave":"Chagas Disease|Evasion strategies|Persistence|Inmunoendocrine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6235,"idioma_id":4,"titulo":"Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199","resumen":"Human embryonic stem cells (hESCs) are hypersensitive to genotoxic stress and display lower survival ability relative to their differentiated progeny. Herein, we attempted to investigate the source of this difference by comparing the DNA damage responses triggered by the topoisomerase I inhibitor camptothecin, in hESCs, human induced pluripotent stem cells (hiPSCs) and hESCs-derived neuroprogenitors (NP). We observed that upon camptothecin exposure pluripotent stem cells underwent apoptosis more swiftly and at a higher rate than differentiated cells. However, the cellular response encompassing ataxia-telangiectasia mutated kinase activation and p53 phosphorylation both on serine 15 as well as on serine 46 resulted very similar among the aforementioned cell types. Importantly, we observed that hESCs and hiPSCs express lower levels of the anti-apoptotic protein Bcl-2 than NP. To assess whether Bcl-2 abundance could account for this differential response we treated cells with ABT-263, WEHI-539 and ABT-199, small molecules that preferentially target the BH3-binding pocket of Bcl-xL and\/or Bcl-2 and reduce their ability to sequester pro-apoptotic factors. We found that in the absence of stress stimuli, NP exhibited a higher sensitivity to ABT- 263 and WEHI-539 than hESCs and hiPSCs. Conversely, all tested cell types appeared to be highly resistant to the Bcl-2 specific inhibitor, ABT-199. However, in all cases we determined that ABT-263 or WEHI-539 treatment exacerbated camptothecin-induced apoptosis. Importantly, similar responses were observed after siRNA-mediated down-regulation of Bcl-xL or Bcl-2. Taken together, our results suggest that Bcl-xL contrary to Bcl-2 contributes to ensure cell survival and also functions as a primary suppressor of DNA double-strand brake induced apoptosis both in pluripotent and derived NP cells. The emerging knowledge of the relative dependence of pluripotent and progenitor cells on Bcl-2 and Bcl-xL activities may help to predict cellular responses and potentially manipulate these cells for therapeutic purposes in the near future.","palabras_clave":"celulas madre pluripotentes|apoptosis|Bcl-2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6236,"idioma_id":4,"titulo":"Selection of Trichoderma Strain to Enhanced Cellulase-Poor Xylanase  Production Using Sugarcane Bagasse as Sole Carbon Source under Light","resumen":"Species from fungal genus Trichoderma were found to produce culture broths rich inxylanase activity, an enzyme which plays an essential role for efficient and complete hydrolysis oflignocellulosic biomass. This research aimed to enhance cellulase-poor xylanase production using alow-cost medium. Firstly, several native Trichoderma strains isolated from subtropical environmentsof Misiones (Argentina) were evaluated based on its xylanase producing potential. Among these, T.atroviride LBM 117 produced the highest cellulase-poor xylanase enzymes, 29 U mL-1 and 28 U mL-1 in experiment with and without light, respectively, using sugarcane bagasse as a sole carbonsource. Besides, when experiments were carried out with light, the highest xylanase productionoccurred in shorter incubation period than experiments without it. Xylanase activity wascharacterized in both supernatants showing the maximum xylanase activity at 50 °C and pH 4.8.Results of electrophoresis on polyacrylamide gels showed two isoenzymes of 105 and 150 KDa insupernatants from experiment with light and only one isoenzyme of 105 KDa from experiment indarkness.","palabras_clave":"TRICHODERMA|XYLANASES|SUGARCANE BAGASSE (SCB)|LIGHT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6237,"idioma_id":4,"titulo":"Thermogravimetric analysis as a method to characterize the agro- industrial wastes used in thermal process","resumen":"The use of biodiesel is becoming more common worldwide. Thisenergy source has several benefits that promote its acceptance; itisbio-renewable, non toxic and biodegradable. In order to reliablypredictits behavior as a fuel, their characterization is necessary.Theexperimental determination of ultimate analysis data requirespecialinstrumentation, while proximate analysis data can be obtainedeasily byusing common equipment but, the required time is high.In thiswork, a methodology is applied based on thermogravimetricanalysis, DTGdeconvolution and empirical correlations for characterizingthe regionalagro-industrial wastes. This methodology allows determining,approximately,the main parameters required for industrial operation in ashort time.These parameters are the high heating value, the contents ofmoisture,volatiles matter, fixed carbon, ashes, carbon, hydrogen,oxygen, lignine,cellulose and hemicellulose. These obtained results aresimilar to theobtained results using standard techniques.","palabras_clave":"METHODS OF ANALYSIS|CHARACTERIZATION|AGRO-INDUSTRIAL WASTES|THERMOGRAVIMETRY ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6238,"idioma_id":5,"titulo":"Evaluación de la transmisión galactógena de Trichinella patagoniensis en ratones BALB\/c","resumen":"Since 1916 to date, it has been suspected that vertical transmission of parasites from the genus Trichinella could occur in pregnant or lactating women during the parenteral phase of infection. The aim of the present study was to evaluate the transmammary transmission of T. patagoniensis in BALB\/c mice. Twenty 7-week-old BALB\/c mice were distributed into two groups of 10 individuals each, depending on the time of gestation when they were infected, 15 or 18 days after detection of the vaginal plug. Each group was subdivided into two subgroups of 5 mice each, which were given an oral dose of 100 or 500 infective larvae respectively.","palabras_clave":"Vertical transmission|Trichinella patagoniensis|Congenital trichinellosis|Balb C"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6239,"idioma_id":4,"titulo":"Hazard, vulnerability and coastal erosion risk assessment in Necochea Municipality, Buenos Aires Province, Argentina","resumen":"This contribution determines the coastal erosion risk of Necochea and Quequén cities, Necochea Municipality, Buenos Aires Province (Argentina). Both risk components, hazard and vulnerability, were assessed by the construction of indices. The hazard index is composed of four indicators: the erosion or accretion rate, coastal geomorphology, storm waves effects, and sediment supply. The vulnerability index is comprised of land use\/cover, demographic, life conditions, and work and consumption indicators, and includes population census data such as demographic, education, health, sanitary, economic, production, work and population exposure aspects. The analysis concluded that coastal erosion risk ranges from very low to high along the study area, Quequén yielding the highest values. Risk levels vary in both cities based on the uneven spatial distribution of hazard. The risk assessment developed herein constitutes a practical and adequate tool that can be utilized with other elements and tasks in the elaboration of a coastal management program.","palabras_clave":"COASTAL EROSION|HAZARD|NECOCHEA|QUEQU&Atilde;&copy;N|RISK|VULNERABILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6241,"idioma_id":4,"titulo":"Photoluminescence, Unconventional-Range Temperature Sensing, and Efficient Catalytic Activities of Lanthanide Metal-Organic Frameworks","resumen":"Abstract: A luminescent lanthanide metal-organic framework based on Eu3+, Tb3+ and 2-phenylsuccinate (EuTb-psa) was solvothermally obtained to explore its behavior as a temperature sensor in a non-conventional temperature range, by studying the thermal evolution of both the hypersensitive 4f-4f transition 5D0&#8594;7F2 and the luminescence lifetime. The efficiency of the luminescence process was evaluated by the europium intrinsic quantum yield (QEu) and its variation as a function of temperature is also reported. The optical properties of four isostructural compounds (Pr-psa, Nd-psa, Gd-psa and Tb-psa) were investigated and compared with related ones. The energy of the triplet state of the ligand was also estimated. The samples were fully characterized by single-crystal and powder X-ray diffraction, thermal analysis and vibrational spectroscopy. The catalytic activity of a series of Ln-psa MOFs (with Ln = Sm, Eu, Gd, Tb, EuGd and EuTb) as heterogeneous catalysts was evaluated in a one-pot cyanosilylation of benzaldehyde as substrate.","palabras_clave":"PHOTOLUMINESCENCE|CATALYSIS|LANTHANIDES|SENSOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6242,"idioma_id":4,"titulo":"Tuning the Structure, dimensionality and Luminescent properties of Lanthanide Metal-Organic Frameworks under Ancillary Ligand Influence","resumen":"This manuscript addresses the synthesis, structural characterization and optical properties of a 1D coordination polymer (CPs) and 2D and 3D Metal-Organic Frameworks (MOFs) obtained from lanthanide metals, 3-Hydroxinaftalene-2,7-disulfonic acid (3-OHNDS) and two different phenanthroline derivates as ancillary ligands. The first is a family of 2D compounds with formula [Ln(3-OHNDS)(H2O)2], where Ln = La(1), Pr(2), Nd(3) and Sm(4). The addition of 1,10-phenanthroline (phen) in the reaction produces 1D compounds with general formula [Ln(3-OHNDS)(phen)(H2O)]?3H2O, where Ln= La(5), Pr(6), Nd(7) and Sm(8). Finally, the synthesis with 3,4,7,8-tetramethyl-1,10-phenanthroline (3,4,7,8-TMPhen) as an ancillary ligand results in the formation of the 3D [La(3-OHNDS)(3,4,7,8-TMphen)(H2O)] (9) compound. The photoluminescence (PL) properties of 1D and 2D compounds were fully investigated in comparison with the 3-OHNDS ligand. One of the most important results was the obtentionof a white-light single-emitter without adding dopant atoms in the structure. With all these results in mind was possible establish structure-properties relationships.","palabras_clave":"LANTHANIDES|MOF|LUMINESCENCE|COORDINATION POLYMERS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6243,"idioma_id":5,"titulo":"LA ZONA DE HOLMOGRAPTUS SPINOSUS (DARRIWILIANO MEDIO) EN LA SIERRA DE VILLICUM, PRECORDILLERA ORIENTAL, PROVINCIA DE SAN JUAN","resumen":"Se registra por primera vezpara Argentina la Zona de Holmograptusspinosus en la parte cuspidal de la FormaciónGualcamayo, aflorante en la quebradade La Pola, sierra de Villicum, PrecordilleraOriental. El material fosilífero procede depelitas negras y nódulos carbonáticos próximosal contacto con la Formación La Cantera.Entre los taxones que integran esta asociaciónse destacan Holmograptus spinosus,Atopograptus woodwardi, Bergstroemograptuscrawfordi y abundantes biseriados correspondientesprincipalmente a los géneros Archiclimacograptusy Haddingograptus. Laasociación permite inferir una edad darriwilianamedia para los niveles portadores.Los taxones At. woodwardi y H. spinosus sonregistrados por primera vez para la Precordillera.","palabras_clave":"Graptolitos|Formación Gualcamayo|Ordovícico Medio|Precordillera argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6244,"idioma_id":4,"titulo":"Storage of Cohniella cepula (Orchidaceae) Pollinia: Fertilizing Ability and Subsequent Fruit and Seed Formation","resumen":"Pollen storage is of great importance for plant breeding and production besides an efficient means for preservation of haploid gene pool of plant genetic resources and rare or endangered species. Pollinia of Cohniella cepula were stored over one year at 4, -20, -70, and -196 ºC. Fertilizing ability of fresh and stored (30 to 360 days) pollinia was determined by the fruit and seed formation for each treatment, as well as by the seed viability, in vitro seed germination, and seedling growth. Pollinia stored at -70 and -196 ºC showed high fertilizing ability (94.4 to 100.0%) even one year after collection, revealing no significant differences with fresh pollinia. Seeds from all treatments showed high viability (91.2 to 94.3%) through the 2,3,5-triphenyltetrazolium chloride reduction assay and high in vitro germination (91.7 to 97.3%). Thus, successful ultra cold storage of C. cepula pollinia was feasible without any desiccation, cryoprotection, or precooling treatment before placing into an ultra freezer (-70 ºC) or immersing in LN (-196 ºC).","palabras_clave":"LONG-TERM STORAGE|LIQUID NITROGEN|POLLEN|IN VITRO SEED GERMINATION|ORCHIDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6245,"idioma_id":5,"titulo":"Nuevos análisis paleoambientales y sus implicancias arqueológicas durante la Pequeña Edad de Hielo en la Puna","resumen":"Resumen  Este trabajo completa los estudios paleoambientales en la Quebrada de Lapao, Puna Seca de Jujuy Argentina, para el período de la Pequeña Edad de Hielo. Las líneas de evidencias principales que se utilizaron fueron los análisis polínicos y sedimentológicos. Sobre esta base de datos y a partir de los estudios etnográficos sobre sociedades pastoriles en ambientes desérticos, se evalúan las estrategias que podrían haber generado el registro arqueológico correspondiente a dicho período. Los resultados paleoambientales obtenidos son coherentes con los antecedentes detectados en otras localidades del área andina, señalando un primer momento (1538-1665 cal. DC) de presencia de una vega impactada, en el marco de una estepa mixta herbácea-arbustiva. Un segundo momento, (1681-1744 cal. DC), de expansión de la vega en el marco de una estepa arbustiva que indicaría condiciones de mayor aridez regional. Y un tercer momento, (1760-1966 cal. DC), de retracción de la vega, en el marco de una estepa mixta. Las expectativas arqueológicas para el momento de aridez detectado, proponen el desarrollo de una estrategia de manejo de camélidos, a partir de una matanza selectiva de individuos de edad adulta. Pequeña Edad de Hielo ? Arqueología ? polen ? sedimentología ? Puna Seca argentina.","palabras_clave":"Puna|Paleoambiente|Polen|Pequeña Edad de Hielo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6246,"idioma_id":5,"titulo":"PARTICIPACIÓN MILITAR ARGENTINA EN MISIONES DE PAZ. DESAFÍOS PARA LA POLÍTICA DE DEFENSA E INFLUENCIA EN LA ORGANIZACIÓN Y FUNCIONAMIENTO EN LAS FUERZAS ARMADAS. 1990-2013","resumen":"Este artículo tiene por objetivo analizar la participación argentina en misiones de paz de Naciones Unidas desde 1990 hasta 2013, determinando: 1)regiones y países en las que se involucró; 2) perfiles de los efectivos enviados; 3) evolución cuantitativa total anual de la participación militar y policial; 4) tipo de actividades realizadas por el personal militar del Ejército Argentino; 5) impacto de la participación militar en la organización interna, alistamiento y adiestramiento del personal del Ejército ?que aportó los contingentes cuantitativamente más importantes en el caso de las Fuerzas Armadas de este país.","palabras_clave":"Defensa|Fuerzas Armadas|Misiones de Paz|Argentina - Naciones Unidas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6247,"idioma_id":4,"titulo":"Analysis and design of a tilted rotor hexacopter for fault tolerance","resumen":"A proof is presented, of how an hexagon shaped hexacopter can be designed in order to keep the ability to reject disturbance torques in all directions while counteracting the effect of a failure in any of its motors. The method proposed  is simpler than previous solutions since it does not require to change the motor rotation direction or any in-flight  mechanical reconfiguration of the vehicle. It consists in tilting the rotor a small fixed angle with respect  to the vertical axis. Design guidelines are presented to calculate the tilt angle in order to achieve fault tolerant attitude control without loosing any significant vertical thrust.It is also formally proved  that the minimum number of unidirectional rotating motors needed to have fault tolerance is six, and that this can be achieved by tilting their rotors. This proof is essentially a control allocation analysis which recovers in a very simple way a result already known: the standard configuration (without tilting the motors) is not fault tolerant. A simulation example illustrates the theory.","palabras_clave":"hexacopter|fault tolerance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6248,"idioma_id":4,"titulo":"Redescription of Antarctophthirus lobodontis (Anoplura: Echinophthiriidae) from the crabeater seal and identification key for Antarctic lice","resumen":"The Anoplura (Phthiraptera) is composed of lice parasitizing mainly terrestrial mammals, but a few members have been able to adapt to the marine environment. The latter are included in the family Echinophthiriidae, a particular group infecting pinnipeds worldwide. They also are of the few insects that managed to survive in the ocean. The study of Antarctic echinophthiriids flourished in the 1960s, but in the last 50 years, no advance has been made. Revision of Antarctic echinophthiriids is part of ongoing research on the systematics, phylogeny and ecology of these lice. During the summer season of 2014, we had the opportunity to collect lice from crabeater seals captured at Cierva Cove in the northern sector of the Danco Coast, Antarctic Peninsula. Since its original description was incomplete and the holotypes were lost, here we redescribe Antarctophthirus lobodontis based on these specimens. The present material can be distinguished from other Antarctic Antarctophthirus species by the presence of four marginal long hairs and in the basis of the head a line of eight spines and three hairs above the last row of four spines. Also in the present work, we provide a key to the identification of the Antarctic species of Echinophthiriidae based on morphological characteristics.","palabras_clave":"ANTARCTICA  |ANTARCTOPHTHIRUS|SUCKING LICE |TAXONOMIC REVISION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6249,"idioma_id":4,"titulo":"Tracing the geographical origin of Argentinean lemon juices based on trace element profiles using advanced chemometric techniques","resumen":"This study examines the application of chemometric techniques associatedwith trace element concentrations fororigin evaluation of lemon juice samples. Seventy-four lemon juice samples from three different provinces ofArgentina were evaluated according to their microelement contents to identify differences in patterns of elementsin the three provinces. Inductively coupled plasmamass spectrometry (ICP-MS)was used for the determinationof twenty-five elements (Ag, Al, As, Ba, Bi, Co, Cr, Cu, Fe, Ga, In, La, Li,Mn, Mo, Ni, Rb, Sb, Sc, Se, Sn, Sr, Tl, V,and Zn). Once the analytical data were collected, supervised pattern recognition techniqueswere applied to constructclassification\/discrimination rules to predict the origin of samples on the basis of their profiles of trace elements.Namely, linear discriminant analysis (LDA), partial least square discriminant analysis (PLS-DA), knearestneighbors (k-NN), random forest (RF), and support vector machine with radial basis function Kernel(SVM). The results indicated that itwas feasible to attribute unknown lemon juice samples to its geographical origin.SVMhad better performance compared to RF, k-NN, LDA and PLS-DA, listed in descending order. Eventually,this study verifies that trace element pattern is a powerful geographical indicator when identifying the origin oflemon juice samples by analyzing trace element data with the help of SVMtechnique. This level of accuracy providesan interesting foundation to propose the combination of trace element contents with SVM technique as avaluable tool to evaluate the geographical origin of lemon juice samples produced in Argentina.","palabras_clave":"Citrus limon|Multi-element analysis|Geographical origin|ICP-MS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6250,"idioma_id":4,"titulo":"Big fish (and a smallish skate) eat small fish: diet variation and trophic level of Sympterygia acuta, a medium-sized skate high in the food web","resumen":"p { margin-bottom: 0.25cm; direction: ltr; color: rgb(0, 0, 10); line-height: 120%; text-align: left; widows: 2; orphans: 2; }p.western { font-family: 'Calibri',serif; font-size: 11pt; }p.cjk { font-family: 'Droid Sans Fallback'; font-size: 11pt; }p.ctl { font-family: 'DejaVu Sans'; font-size: 11pt; }a:link { color: rgb(0, 0, 255); }        The bignose  fanskate, Sympterygia  acuta,  is a small-to-medium-sized  species endemic  to  shallow coastal waters of  the  Southwest Atlantic.  S.  acuta  displays a clear seasonal reproductive cycle, characterized by a  maximum egg laying activity in spring and hatching in summer.  We hypothesize that diet and feeding activity change with maturity  stage and season and that, given  its smallish size,  the trophic level is low. Using a multiple-hypothesis modeling  approach,  the diet of S.  acuta  in relation with sex, body size, maturity stage, region (i.e.  north and south)  and season was analyzed; and a potential relationship between feeding  activity and the seasonal reproductive cycle was assessed.  S. acuta  fed on a broad spectrum of prey, but teleosts were more important  (47.97%  index  relative    importance , %IRI)  followed by decapods (39.84 %IRI), cumaceans (8.31 %IRI) and isopods  (1.89 %IRI). Maturity stage was a strong determinant of the  ontogenetic diet shift of S.  acuta,  associated to region and season. Feeding  activity was higher in the cold season than in the warm season, and  was less important in the south region than in the north region.  Unexpectedly,  the  specific trophic level was high (3.87). S.  acuta  shifts its diet with maturity stage possibly by a combination of an  improved ability to capture prey and a change in energy demand of  mature individuals. Despite  being  a small-to-medium-sized  skate,S.  acutashowed  a trophic level similar to that  oflarge-bodied  marine predators.  It reduces its feeding activity seasonally, because in the warm  season this species may experience and increased predation risk from  large sharks. ","palabras_clave":"RAJIDAE|ONTOGENETIC SHIFTS|FEEDING ACTIVITY|SOUTHWEST ATLANTIC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6251,"idioma_id":4,"titulo":"A regional water balance indicator inferred from satellite images of an Andean endorheic basin in central-western Argentina","resumen":"In the Central Andes of Argentina (30°?37°S), snowmelt is the main source of freshwater, an essential natural resource for ~2.2 million people in the adjacent arid lowlands. In this region, Laguna Llancanelo collects the water inputs from the Malargüe endorheic basin. Previous studies concerning the annual and intra-annual variations of this lagoon and its relationship with regional climate are rare. We obtained a monthly record of Laguna Llancanelo Area (LLA, 1984?2013) using the Modified Normalized Difference Water Index derived from Landsat images. Monthly LLA ranges between 35 and 411 km2 and is significantly related to Río Malargüe variations, the main snow-fed tributary river to the lagoon. There is no long-term relationship between LLA and local rainfall, but rapid increases in LLA result from heavy rainfall around the lagoon. Conversely, rapid reductions in LLA encompass periods with both reduced discharge from Río Malargüe and low local rainfall. The LLA integrates moisture from both Pacific (snowfall in the upper Andes) and Atlantic (lowland rainfall) origins; therefore, we propose to use LLA as an indicator of regional water balance.","palabras_clave":"LAGOON AREA FLUCTUATIONS|REMOTE SENSING|HYDROMETEOROLOGICAL INDEX|CLIMATE VARIABILITY|SOUTHERN ANDES|LAGUNA LLANCANELO|RAMSAR SITE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6252,"idioma_id":4,"titulo":"Effects of rumen-protected methionine and choline supplementation on the preimplantation embryo in Holstein cows","resumen":"Our objective was to determine the effects of supplementing methionine and choline during the pre and postpartum periods on preimplantation embryos of Holstein cows. Multiparous cows were assigned in a randomized complete block design into four treatments from 21 days before calving to 30 days in milk (DIM). Treatments (TRT) were: MET [n = 9, fed the basal diet + rumen-protected methionine (RPM) at a rate of 0.08% (w\/w) of the dry matter (DM), Smartamine® M], CHO (n = 8, fed the basal diet + choline 60 g\/day, Reashure®), MIX (n = 11, fed the basal diet + Smartamine® M and 60 g\/day Reashure®) and CON (n = 8, no supplementation, fed the close-up and fresh cow diets). Cows were randomly re-assigned to two new groups (GRP) to receive the following diets from 31 to 72 DIM; CNT (control, n = 16, fed a basal diet) and SMT [n = 20, fed the basal diet + 0.08% (w\/w) of the DM intake as methionine]. A CIDR® device was inserted in all cows after follicular aspiration (60 DIM) and superovulation began at day 61.5 using FSH in 8 decreasing doses at 12 h intervals over a four days period. On days 63 and 64 all cows received two injections of PGF2&#945; and CIDR® was removed on day 65. Twenty-four h after CIDR® removal, ovulation was induced with GnRH. Cows received AI at 12 h and 24 h after GnRH. Embryos were flushed 6.5 days after AI. Global methylation of the embryos was assessed by immunofluorescent labeling of 5-methylcytosine, while lipid content was assessed by staining with Nile red. Nuclear staining was used to count the total number of cells per embryo. There was no difference between TRT, GRP or their interaction (P > 0.05) for embryo recovery, embryos recovered, embryo quality, embryo stage or cells per embryo. Methylation of the DNA was not different among TRT but there was a TRT by GRP interaction (P = 0.01). Embryos from cows in CON-CNT had greater (P = 0.04) methylation [0.87 ± 0.09 arbitrary units (AU)] than embryos from cows in MET-CNT (0.44 ± 0.07 AU). The cytoplasmic lipid content was not affected (P > 0.05) by TRT or their interaction, but lipid content was greater (P = 0.04) for SMT (7.02 ± 1.03 AU) than in CNT (3.61 ± 1.20 AU). In conclusion, cows in MET-CNT had embryos with lower methylation and SMT cows had a higher lipid content than CNT. Methionine supplementation seems to impact the preimplantation embryo in a way that enhances its capacity for survival since there is strong evidence that endogenous lipid reserves serve as an energy substrate.","palabras_clave":"METHIONINE|CHOLINE|METHYLATION|LIPID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6253,"idioma_id":5,"titulo":"Reducción del drenaje profundo y la lixiviación de nitrógeno en rotaciones agrícolas con cultivos de cobertura","resumen":"Los cambios de vegetación pueden alterar los flujos de agua y nitrógeno (N) de los ecosistemas. La intensificación de las rotaciones con cultivos de cobertura (CC) otoño-invernales reduce el N del suelo y el riesgo de lixiviación durante los excesos hídricos. El objetivo de este trabajo fue contrastar el drenaje profundo y la lixiviación de N en una rotación soja-maíz con y sin CC utilizando lisímetros. Se cultivó avena antes de soja y avena+vicia antes de maíz. Las precipitaciones fueron de 732 y 562 mm durante los períodos intercultivo 2014 y 2015 y de 817 y 705 mm durante los cultivos de soja y maíz, respectivamente. La inclusión de CC redujo el drenaje durante los períodos intercultivo (del 41 al 25% y del 22 al 9% de la precipitación durante el primer y segundo período intercultivo, respectivamente) y durante el cultivo de soja (del 18 al 14% de la precipitación). Durante el cultivo de maíz el drenaje fue similar en las dos rotaciones, representando el 14% de la precipitación. La concentración media de N-NO3&#713; del drenaje fue de 6 y 15 mg\/L para la rotación con y sin CC, respectivamente, y la inclusión de CC redujo la lixiviación de 35 a 14 y de 17 a 3 kg N\/ha durante los períodos intercultivo 2014 y 2015, respectivamente, y de 36 a 3 y de 15 a 6 kg N\/ha durante los cultivos de soja y maíz, respectivamente. Estas reducciones, sumadas al aporte adicional de N por fijación biológica de vicia, produjeron balances de N positivos o cercanos a neutro mientras que la rotación sin CC presentó balances negativos. Además de mejorar la circulación interna de N, las reducciones en el drenaje con CC se pueden traducir en menor recarga del acuífero y contribuir a la regulación de inundaciones durante períodos lluviosos.","palabras_clave":"Lisímetros de relleno|Balance hídrico|Balance de nitrógeno|Nivel freático"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6254,"idioma_id":4,"titulo":"Impact of Universal Health Coverage on Child Growth and Nutrition in Argentina","resumen":"Objectives. To estimate trends of undernutrition (stunting and underweight) among children younger than 5 years covered by the universal health coverage programs Plan Nacer and Programa Sumar.Methods. From 2005 to 2013, Plan Nacer and Programa Sumar collected high-quality information on birth and visit dates, age (in days), gender, weight (in kg), and height (in cm) for 1.4 million children in 6386 health centers (13 million records) with broad coverage of vulnerable populations in Argentina.Results. The prevalence of stunting and underweight decreased 45.0% (from 20.6% to 11.3%) and 38.0% (from 4.0% to 2.5%), respectively, with differences between rural versus urban areas, gender, regions, age, and seasons.Conclusions. Undernutrition prevalence substantially decreased in 2 programs in Argentina as a result of universal health coverage.Read More: http:\/\/ajph.aphapublications.org\/doi\/abs\/10.2105\/AJPH.2016.303056","palabras_clave":"stunting|underweight|Universal Health Coverage|Undernutrition"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6255,"idioma_id":4,"titulo":"Connexin 26 (GJB2) mutation in an Argentinean patient with keratitis-ichthyosis-deafness (KID) syndrome: a case report","resumen":"Background: Keratitis-Ichthyosis-Deafness (KID) syndrome is a rare condition characterized by pre-lingual sensorineuraldeafness with skin hyperkeratinization. The primary cause of the disease is a loss-of-function mutation in the GJB2gene. Mutations in Argentinean patients have not been described.Case presentation: We studied a 2 year-old boy with bilateral congenital sensorineural deafness with dry skin over theentire body, hypotrichosis of the scalp, thin and light-blond hair. Analysis of the GJB2 gene nucleotide sequence revealedthe substitution of guanine-148 by adenine predicted to result in an Asp50Asn amino acid substitution.Conclusion: This is the first KID report in a patient from Argentina. This de novo mutation proved to be the cause ofkeratitis-ichthyosis-deafness syndrome (KID-syndrome) in the patient, and has implications in medical genetic practice.Keywords: GJB2, Mutations, KID syndrome, Connexin, Deafness, p.Asp50Asn","palabras_clave":"dermatitis|hipoacusia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6256,"idioma_id":4,"titulo":"Editorial: Auditory Efferent System: New Insights from Cortex to Cochlea.","resumen":"sin resumen","palabras_clave":"cóclea|audición"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6257,"idioma_id":4,"titulo":"Forecasting the fate of high mountain ponds in the Andean region under future climate change","resumen":"Theaims of this study are: (i) to identify areas in the Andean Region where theclimate will remain stable enough for the survival of the study species; (ii)to analyze how climate change will affect these areas under different climatescenarios; (iii) to generate spatially explicit predictive maps of the expansionor retraction of these areas; and (iv) based on this information, to identifyareas with priority for conservation. The analysis was performed using presenceonly data for 14 Heteroptera and Odonata species. Current and future modelswere developed to identify areas where the climate would be suitable for smallponds, using Maxent v3.3.3k, with future models based on three different GCMsfor the 2050 period (scenarios A2a and B2a). Model performance was evaluatedusing the jackknife approach. Climatic niche breadth and climatic nichesimilarities were calculated through Levin?s concentration metrics and the Istatistic index (implemented in ENMTools), respectively. Maxent logisticoutputs were converted into binary presence\/absence maps, based on the ?minimumtraining presence logistic threshold? and used to build species richness mapsfor each condition considered (present and future). Current and future modelswith areas climatically suitable for small ponds were developed. All the study speciesproved to be narrow specialists and share similar climatic spaces. Ourprojections suggest that four of the species would not find suitable climateconditions for survival in the future. The priority area for conservation,where most species would find suitable climate conditions, is located between33-47º south and 73º-70º west. We identified future loss of the priority areatowards the east and a small gain towards the north and south. The mostprobable situation for the year 2050 is a negativeprecipitation-evapotranspiration balance, and small ponds will probably be veryshort-lived or dry completely during summer, suggesting a drastic change inspecies assemblages and species richness of the region, which could become ahotspot of extinction.","palabras_clave":"AREAS OF HIGH ENDEMISM|GREEN HOUSE EFFECT|SPECIES DISTRIBUTIONAL MODELS (SMD)|HEMIPTERA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6258,"idioma_id":4,"titulo":"Heritability of fear of humans in urban and rural populations of a bird species","resumen":"Flight initiation distance (FID), a measure of ananimal's tolerance to human disturbance and a descriptor of its fear of humans,is being increasingly employed forconservation purposes and to predict the response of species towardurbanization. However, most work devoted to understand variability on FID hasbeen conducted at the population level and little is still known oninter-individual variability in this behaviour. We estimated the heredabilityof FID, a key point to understand the strength and evolutionary consequences ofselection of particular phenotypes associated to human disturbances, using along-term monitored population of burrowing owls (Athene cunicularia) for which FID was previously proven to behighly consistent across the individual?s lifespan. Heritability estimates variedbetween 0.37 and 0.80, depending on the habitat considered (urban-rural) andmethod used (parent-offspring regressions or animal models). These values are unusuallyhigh compared with those previously reported for otherbehavioural traits. Although more research is needed to fully understand theunderlying causes of this resemblance between relatives, selection pressuresacting on this behaviour should be seriously considered as an importantevolutionary force in animal populations increasingly exposed to humandisturbance worldwide.","palabras_clave":"FLIGHT INITIATION DISTANCE|HERITABILITY|PARENT-OFFSPRING REGRESSIONS|ANIMAL MODELS|URBANIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6259,"idioma_id":4,"titulo":"Rab3A,  a  possible  marker  of  cortical  granules, participates in cortical granule exocytosis in mouse eggs","resumen":"Fusion of cortical granules with the oocyte plasma membrane is the most significant event to prevent polyspermy. This particular exocytosis, also known as cortical reaction, is regulated by calcium and its molecular mechanism is still not known. Rab3A, a member of the small GTP-binding protein superfamily, has been implicated in calcium-dependent exocytosis and is not yet clear whether Rab3A participates in cortical granules exocytosis. Here, we examine the involvement of Rab3A in the physiology of cortical granules, particularly, in their distribution during oocyte maturation and activation, and their participation in membrane fusion during cortical granule exocytosis. Immunofluorescence and Western blot analysis showed that Rab3A has a similar migration pattern to cortical granules during oocyte maturation, and that Rab3A is not longer detected after cortical granule exocytosis. These results suggested that Rab3A might be a marker of cortical granules. Overexpression of EGFP-Rab3A colocalized with cortical granules with a Pearson correlation coefficient of +0.967, indicating that Rab3A and cortical granules have almost a perfect colocalization in eggs. Microinjection of recombinant, prenylated and active GST-Rab3A triggered cortical granule exocytosis, indicating that Rab3A has an active role in this secretory pathway. To confirm this active role, we inhibited the function of endogenous Rab3A by microinjecting a polyclonal antibody raised against Rab3A prior to parthenogenetic activation. Our results showed that Rab3A antibody microinjection abolished cortical granule exocytosis in parthenogenetically activated oocytes. Altogether, our findings confirm that Rab3A participates in cortical reaction and might function as a marker of cortical granules membrane in mouse eggs during oocyte maturation.","palabras_clave":"Rab3A|cortical granules|cortical reaction|ovocyte"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6260,"idioma_id":4,"titulo":"A note on path domination","resumen":"We study domination between different types of walks  connectingtwo non-adjacent vertices $u$ and $v$ of a graph (shortest paths,induced paths, paths, tolled walks). We succeeded incharacterizing those graphs in which every $uv$-walk of oneparticular kind  dominates every $uv$-walk of other specific kind.We thereby obtained new characterizations of standard graphclasses like chordal, interval and superfragile graphs.","palabras_clave":"domination|paths|characterization"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6261,"idioma_id":4,"titulo":"A global assessment of the conservation status, threats and priorities for albatrosses and large petrels","resumen":"Seabirds areamongst the most globally-threatened of all groups of birds, and &nbsp;conservation issues specific toalbatrosses (Diomedeidae) and large petrels &nbsp;(Procellaria spp. and giant petrels Macronectes spp.)led to drafting of the multi-lateralAgreement on the Conservation ofAlbatrosses and Petrels (ACAP). Here we review &nbsp;the taxonomy, breeding and foraging distributions,population status and trends, threats and priorities for the 29 species coveredby ACAP. Nineteen (66%) are listed &nbsp;as threatened by IUCN, and 11 (38%) are declining. Most haveextensive at-sea distributions, and the greatest threat is incidental mortality(bycatch) in industrial &nbsp;pelagic or demersal longline, trawl or artisanal fisheries,often in both national and &nbsp;international waters. Mitigation measures are available thatreduce bycatch in mosttypes of fisheries, but some management bodies are yet tomake these mandatory, levels of implementation and monitoring of compliance areoften inadequate, and there &nbsp;are insufficient observer programmes collecting robust dataon bycatch rates Intentional take, pollution (including plastic ingestion), andthreats at colonies affect &nbsp;fewer species than bycatch; however, the impacts of diseaseand of predation by &nbsp;introduced species, including feral cats (Felis catus),rats (Rattus spp.) and house &nbsp;mice (Mus musculus), are severe for some breedingpopulations. Although major &nbsp;progress has been made in recent years in reducing bycatchrates and in controlling &nbsp;or eradicating pests at breeding sites, unless conservationefforts are intensified, the &nbsp;future prospects of many species of albatrosses and largepetrels will remain bleak.","palabras_clave":"anthropogenic impacts| conservation management|invasive species|non-target species|population trends|regional fisheries management organisations"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6262,"idioma_id":4,"titulo":"Development of a potentially probiotic food through fermentation of Andean tubers","resumen":"With the aim to obtain a non-dairy solid probiotic product, three different Andean tubers -oca (Oxalis tuberosa), papalisa (Ullucus tuberosus) and potato (Solanum tuberosum spp andigena)- were assessed as fermentation substrates for the potentially probiotic L. brevis CJ25 strain. Fermented Churque~na potato puree, oregano and NaCl were used for the manufacture of a product called Potato Cheese due to its firm texture. This functional food contains a viable cell concentration of 8.0 log CFU\/g and pH 5.1. Even after 28 days of storage at 4 C, increases in cell counts were found and pH decreased 0.7 units, which  improved food safety avoiding the growth of spoilage and pathogenic microorganisms. The in vitro tests indicated that L. brevis CJ25 exhibited high level of survival in simulated gastric juice (3 h, pH 2.5) when delivered in Potato Cheese, and also showed resistance to bile salt after 3 h of exposure. These results suggest that Potato Cheese is a promising novel functional food with high nutritional value, free of cholesterol and lactose and its development may have a favorable impact in Andean regional economies.","palabras_clave":"ANDEAN TUBERS  |LACTOBACILLUS BREVIS|PROBIOTIC VIABILITY|IN VITRO GASTROINTESTINAL SIMULATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6263,"idioma_id":4,"titulo":"Kinetics of color change of osmotically dehydrated chub mackerel (Scomber japonicus) during storage at different temperatures","resumen":"The aim of this study was to determine the effect of storage temperature on the kinetics of color change of chub mackerel dehydrated in a ternary solution (water, glycerol and salt) and vacuum packaged in films. The color of processed fish can change because of lipids and protein oxidation during storage. Samples were stored at 7, 25 and 35°C for seven months and kinetic models of 0, 1 and 2 order were applied to describe the color changes. It was observed that an increase in the storage temperature improved the changes in the CIE color values (L*, a* and b*). First-order reaction had the best statistical parameters for a* at the three temperatures tested. The temperature dependence of parameter a* indicated an Arrhenius relationship and the activation energy (Ea) was 44.33 kJ\/mol. The parameter b* fitted to the proposed models only in samples stored at 35°C. The L* value decreased during storage at 25 and 35ºC (pale to dark). Storage at refrigeration temperature (7°C) minimized the color changes.","palabras_clave":"COLOR|CHUB MACKERELL|OSMOTIC DEHYDRATION|STORAGE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6264,"idioma_id":4,"titulo":"Stabilization by multipoint covalent attachment of a biocatalyst with polygalacturonase activity used for juice clarification","resumen":"Derivatized-agarose supports are suitable for enzyme immobilization by different methods, takingadvantage of different physical, chemical and biological conditions of the protein and the support. In this study, agarose particles were modified with MANAE, PEI and glyoxyl groups and evaluated to stabilize polygalacturonase from Streptomyces halstedii ATCC 10897. A new immobilized biocatalyst was developed using glyoxyl-agarose as support; it exhibited high performance in degrading polygalacturonic acid and releasing oligogalacturonides. Maximal enzyme activity was detected at 5 h of reaction using 0.05 g\/mL of immobilized biocatalyst, which released 3 mg\/mL of reducing sugars and allowed the highest product yield conversion and increased stability. These results are very favorable for pectin degradation with reusability up to 18 successive reactions (90 h) and application in juice clarification. Plum (4.7 Bx) and grape (10.6 Bx) juices were successfully clarified, increasing reducing sugars content and markedly decreasing turbidity and viscosity","palabras_clave":"Glyoxyl agarose|Streptomyces halstedii ATCC 10897|Enzyme purification|Grape and plum juices"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6265,"idioma_id":5,"titulo":"Estilo Personal del Terapeuta en Psicoterapeutas de Adicciones","resumen":"Estudios previos afirman la importancia de investigar el Estilo Personal del Terapeuta (EPT) en psicólogos que se aboquen al tratamiento de grupos específicos de pacientes, como por ejemplo pacientes severos.  El propósito de este artículo fue comparar el Estilo Personal del Terapeuta entre dos grupos de profesionales: uno dedicado a la atención de pacientes drogodependientes (grupo en estudio) y otro a la atención clínica en general. La muestra estuvo formada por 60 profesionales: 30 pertenecientes a cada grupo.  Como resultado se obtuvieron diferencias significativas en la función Expresiva, la cual está relacionada con la comunicación emocional como aspecto nuclear de la empatía, siendo menor en el grupo en estudio.","palabras_clave":"ESTILO PERSONAL DEL TERAPEUTA|PACIENTES DROGODEPENDIENTES|PSICOTERAPIA|RELACIÓN TERAPÉUTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6266,"idioma_id":4,"titulo":"Antifungal drugs combinations: a patent review 2000-2015","resumen":"Introduction: Combination therapy has emerged as an approach to improve the efficacy of antifungal drugs, considering that synergistic interactions can achieve higher antifungal effects with lower quantities of each substance, thus reducing lower toxic side-effects and higher spectrum of activity. Areas covered: Twenty four patents disclosed in the period 2000-2015 were covered in this review: twenty were devoted to pharmacodynamic potentiation, while four were dedicated to pharmacokinetic actions. Expert opinion: The common characteristic of most published patents is that the main partner is a commercial antifungal drug. In the most innovative combinations the second component was either a modifier of proton homeostasis, an antibody, an inhibitor of the adhesion of epithelial or endothelial cells or a keratinolitic agent that improve the skin penetration. The evaluation of synergism is always made with simple in vitro methods, which constitutes a weakness of the disclosed patents, due to the lack of in vivo studies, since the in vitro tests cannot predict the in vivo behavior. Also, it is surprising that none of the patents analyzes the toxicity of the new combinations, taking into account that one of the main objectives of the combinations is to reduce the toxicity of the existing antifungal drugs.","palabras_clave":"ANTIFUNGAL|COMBINATION|SYNERGISM|MYCOSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6267,"idioma_id":4,"titulo":"Provenance and paleogeography of the Devonian Durazno Group, southern Parana Basin in Uruguay","resumen":"A succession of Devonian cover rocks occurs in outcrop and in the subsurface of central-northern Uruguay where they were deposited in an intracratonic basin. This Durazno Group comprises three distinct stratigraphic units, namely the Cerrezuelo, Cordobes and La Paloma formations. The Durazno Group does not exceed 300 m of average thickness and preserves a transgressive-regressive cycle withina shallow-marine siliciclastic shelf platform, and is characterized by an assemblage of invertebrate fossils of Malvinokaffric affinity especially within the Lower Devonian Cordobes shales. The sedimentary provenance of the Durazno Group was determined using petrography, geochemistry, and morphologicalstudies of detrital zircons as well as their UePb ages. Sandstone petrography of Cerrezuelo and La Paloma sequences shows that they have a dominantly quartz-feldspathic composition with a minor contribution of other minerals. Whole-rock geochemical data indicate that alteration was strong in each of the threeformations studied; chondritic-normalized REE patterns essentially parallel to PAAS, the presence of a negative Eu-anomaly, and Th\/Sc and La\/Hf ratios point to an average source composition similar to UCC or slightly more felsic. Within the Cerrezuelo Formation, recycling of older volcano-metasedimentary sources is interpreted from Zr\/Sc ratios and high Hf, Zr, and REE concentrations. UePb detrital zircon age populations of the Cerrezuelo and La Paloma formations indicate that the principal source terranes are of Neoproterozoic age, but include also minor populations derived from Mesoproterozoic and Archean-Paleoproterozoic rocks. A provenance from the Cuchilla Dionisio-Dom Feliciano, Nico Perez and Piedra Alta terranes of Uruguay and southern Brazil is likely. This study establishes an intracratonic extensional tectonic setting during Durazno time. Considering provenance age sources, regional paleocurrentdistributions and the established orogenic history recorded in SW Gondwana, we suggest that the basin fill was derived from paleohighs located in what is currently SE Uruguay.","palabras_clave":"ZIRCONS|DEVONIAN|PALEOGEOGRAPHY|GONDWANA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6268,"idioma_id":4,"titulo":"Impact of the desilication treatment of Y zeolite on the catalytic cracking of bulky hydrocarbon molecules","resumen":"Mesoporosity was induced on a USY zeolite by means of an alkaline leaching process. Samples were immersed in 0.05, 0.10 and 0.20 M NaOH solutions during 15 min at room temperature and then were exchanged with NH4+ ions and calcined to yield the acid forms. The formation of mesopores with size ranging from 20 to 100 Å increased with the concentration of NaOH. The modified zeolites were used at 30 %wt. to formulate cracking catalysts with an inert SiO2 matrix. The catalytic performance of these catalysts in the conversion of 1,3,5-tri-isopropylbenzene was evaluated in a batch, fluidized bed reactor at 450, 500 and 530 °C, with a catalyst to oil relationship of 4.7 and contact times up to 16 s. The catalysts with the modified zeolites were more active in the cracking of these bulky molecules than the one with the parent, unmodified zeolite; moreover, the selectivity to the products of primary cracking reactions increased. These results reveal an enhanced diffusion of the reactant molecules to the zeolite active sites and of the products out of the catalyst particles.","palabras_clave":"MESOPORE|ZEOLITE|DESILICATION|DIFFUSION|CRACKING SELECTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6269,"idioma_id":5,"titulo":"Valve morphology of  Didymosphenia geminata   (Bacillariophyceae) in Chubut province, Argentina","resumen":"Didymosphenia was recently found in Patagonia forming massive proliferations. In Argentinathe species scattered in a short time to five provinces. Here, we analyse morphologic and morphometricdata of specimens collected at Chubut Province, Argentina, and we compare them with other species ofthis genus reported from different regions of the world. Materials were collected in two basins during 2010-2012. Samples analyzed with LM and SEM showed inter and intrapopulation variation in morphometriccharacteristics and in some valve structures such as spines. The studied material corresponds in valveoutline and morphology to D. geminata ssp geminata Metzeltin & Lange-Bertalot. Our materials also resemble a group of species: D. clavaherculis, D. clavamagna, D. coronata and D. laticollis. The studied material can be easily confused to some specimens mentioned in the literature as D. clavaherculis but they clearly differ from the type material of the species. On the other hand, fine morphology of the studied materials coincides with materials from Chile. Although morphological revision of the genus recentlypublished allowed separating many species, there are still doubts about specific limits of taxa closelyrelated to D. geminata. Molecular analysis should be performed to clarify these issues.","palabras_clave":"Diatoms|didymo|Patagonia|morphometry|nuisance species"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6270,"idioma_id":4,"titulo":"The seed-borne transmitted Southern bean mosaic virus hinders the early events of nodulation and growth of Rhizobium-inoculated Phaseolus vulgaris L.","resumen":"Abstract. To simulate seed-borne virus transmission, a noninvasive protocol was designed to infect the radicle ofgerminating seeds, with 100% effectiveness. Preinfection of 24-h-old black bean (Phaseolus vulgaris L.) radicles bySouthern bean mosaic virus (SBMV) followed by Rhizobium inoculation 48 h later caused a drastic reduction in rootnodulation. Results were attributed to active virus replication within the elongating zone of the radicle at least 32 h beforeRhizobium inoculation, which elicited severe anatomical malformations; an abnormal accumulation of apoplastic reactiveoxygen species in the rhizodermis, cortex, inner cortical and endodermic root cells; the formation of atypical root hair tipsand the collapse of 94% of the root hairs in the SBMV-preinfected radicles. Adult SBMV-preinfected plants showedexacerbated virus symptoms and 80% growth reduction ascribed to major virus-induced ultrastructural alterations inthe nodules. The accumulation of ureides, aamino acids and total reducing sugars in the leaves and nodules of SBMVpreinfectedplants are indicators of the hindering effects ofSBMVinfection onN2 fixation and ureide catabolism, causingNstarvation. The exogenous addition of 1 or 4 mM naringenin, genistein or daidzein did not counteract the deleteriouseffects of SBMV preinfection on nodulation.","palabras_clave":"root hairs,|reactive oxygen species|SBMV|nodule ultrastructure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6271,"idioma_id":4,"titulo":"An autophagy-related kinase is essential for Phaseolus vulgaris symbiosis with both Rhizobium and arbuscular endomycorrhizal fungi","resumen":"Eukaryotes contain three types of lipid kinases belonging to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces, only PI3K class III family members have been found. These enzymes regulate senescence, the innate immune response, autophagy and intracellular trafficking. Here, we report that RNAi-mediated down-regulation of P. vulgaris PvPI3K, severely impaired symbiosis in composite common bean plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. When PvPI3K was downregulated, a marked decrease in root hair growth and curling was observed. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were also severely affected. Interestingly, root colonization by AM fungi, the formation of arbuscules was also abrogated in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. Expression of one of these genes, Beclin1 \/ Atg6, was also knocked-down by RNAi, resulting in a similar impaired-symbiosis phenotype as that observed in PvPI3K down-regulated transgenic roots. Our findings show for the first time that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms.","palabras_clave":"gene silencing - RNA interference\/RNAi, |plant-microbe symbiosis| autophagy|root development - root nodules"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6272,"idioma_id":4,"titulo":"Temporal and spatial changes in soil micronutrients in managed Nothofagus pumilio forest of Tierra del Fuego, Argentina","resumen":"Soil organic components are important factorsin the quality and productivity of forest ecosystems. Timberharvesting reduces plant cover and the amount oforganic matter in forest floor layer, increases surface runoff,soil erosion and alters microclimatic conditions overlarge areas. These changes can have important implicationsfor nutrient cycling dynamics and soil organic mattermineralization. Fueguian temperate forests of Nothofaguspumilio (lenga) have been intensely harvested for the lastdecades, mainly by shelterwood-cut silvicultural system.Harvesting removes nutrients contained in logs from thesite, modifies light, temperature and soil humidity, constrainingnutrient cycling process. In this study, we evaluateavailable copper (Cua), zinc (Zna), iron (Fea), andmanganese (Mna) concentrations and reservoirs in standsthat represent a chronosequence and their respective primaryforests (controls): stands cut 1 year ago, stands cut5?10 years ago, and stands harvested more than 50 yearsago. Concentrations of Zna and Cua in primary forest were39.9 and 2.6 mg\/kg, and increased in harvested sites to 60.5 and 3.2 mg\/kg, respectively. Fea and Mna concentrationsshowed similar ranges in both harvested and controlsites. Recent harvested sites showed the highest Cua concentrations.Micronutrient reservoirs showed similar rangesin both harvested and primary forests. We concluded thatmicronutrient availability changes at short term after forestharvesting; thus, the inclusion of soil fertility assessment inforest management plans should be incorporated to preservethe fertility of lenga forests soils and ensuresustainability.","palabras_clave":"zinc|copper|shelterwood-cut silvicultural system|lenga temperate forest"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6273,"idioma_id":4,"titulo":"Performance of Quantized Random Beamforming in Delay-Tolerant Machine-Type Communication","resumen":"Machine-to-Machine (M2M) communication represents a new paradigm for mobile cellular networks, where a massive number of low-cost devices requests the transfer of small amounts of data without human intervention. One option to tackle this problem is obtained by combining Random Beamforming (RBF) with opportunistic scheduling. RBF can be used to induce larger channel fluctuations and opportunistic scheduling can be used to select M2M devices when their overall channel quality is good. Traditional RBF does not fulfill M2M requirements because overall channel quality needs to be tracked continuously. In order tackle this limitation, a novel codebookbased RBF architecture that identifies in advance the time instants in which overall channel quality should be reported, within a coherence time window, is proposed. This opportunistic feedback mechanism reduces signaling overhead and enables energy saving at M2M devices. A simplified methodology is presented to evaluate the system mean data rate, using for this purpose closed form formulas derived from SNR distribution approximations. Results reveal that the performance loss that is experienced for introducing the proposed modifications to traditional RBF scheme is negligible. The concepts analyzed in this paper provide useful insights, and show that codebook-based RBF with simplified opportunistic scheduling algorithms is an excellent combination to provide wide-area M2M services with low-cost devices and limited signaling overhead.","palabras_clave":"Machine-to-machine|Limited feedback|Opportunistic Scheduling|Range Extension"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6274,"idioma_id":4,"titulo":"Brain cortex mitochondrial bioenergetics in synaptosomes and non-synaptic mitochondria during aging","resumen":"Alterations in mitochondrial bioenergetics have been associated with brain aging. In order to evaluate the susceptibility of brain cortex synaptosomes and non-synaptic mitochondria to aging-dependent dysfunction, male Swiss mice of 3 or 17 months old were used. Mitochondrial function was evaluated by oxygen consumption, mitochondrial membrane potential and respiratory complexes activity, together with UCP-2 protein expression. Basal respiration and respiration driving proton leak were decreased by 26% and 33% in synaptosomes from 17-months old mice, but spare respiratory capacity was not modified by aging. Succinate supported state 3 respiratory rate was decreased by 45% in brain cortex non-synaptic mitochondria from 17-month-old mice, as compared with young animals, but respiratory control was not affected.Synaptosomal mitochondria would be susceptible to undergo calcium-induced depolarization in 17 months-old mice, while non-synaptic mitochondria would not be affected by calcium overload.UCP-2 was significantly up-regulated in both synaptosomal and submitochondrial membranes from 17-months old mice, compared to young animals. UCP-2 upregulation seems to be a possible mechanism by which mitochondria would be resistant to suffer oxidative damage during aging.","palabras_clave":"SYNAPTOSOMES|NON-SYNAPTIC MITOCHONDRIA|AGING|CEREBRAL CORTEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6275,"idioma_id":4,"titulo":"Analysis of Internal and External Validity Criteria for a Computerized Visual Search Task. A pilot study.","resumen":"Inhibition is one of the main executivefunctions, because of its fundamental role in cognitive and social development.Given the importance of reliable and computerized measurements to assessment inhibitoryperformance, this research intends to analyze the internal and externalcriteria of validity of a computerized conjunction search task, to evaluate therole of perceptual inhibition. A sample of 41 children (21 females and 20males), aged between 6 and 11 years old (M = 8.49, SD = 1.47), intentionallyselected from a private management school of Mar del Plata (Argentina), middlesocio-economic level were assessed. The Conjunction Search Task from the TAC Battery, Coding and Symbol Search tasks from Wechsler Intelligence Scale forChildren were used. Overall, results allow us to confirm that the perceptualinhibition task form TAC presents solid rates of internal and external validitythat make a valid measurement instrument of this process.","palabras_clave":"PERCEPTUAL INHIBITION|CONJUNCTION SEARCH TASK|TAC|VALIDITY|CHILDREN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6276,"idioma_id":4,"titulo":"Molecular Diagnostic of Both Brown and Orange Sugarcane Rust and Evaluation of Sugarcane Brown Rust Resistance in Tucuman, Argentina, Using Molecular Markers Associated with Bru1 a Broad-Range Resistance Allele","resumen":"In April 2015, lesions typical of orange rust were observed in acommercial sugarcane field in Fachinal, Misiones Province, Argentina, on anonidentified cultivar (a complex hybrid of Saccharum spp.). Symptoms onleaves differed from those of brown rust of sugarcane (Pucciniamelanocephala), commonly observed in the region. Leaf samples wereanalyzed both at the EEAOC and INTA Plant Pathology laboratories inTucumán, Argentina. Light microscope studies revealed that uredinia andurediniospore were similar morphologically to those described for thefungus Puccinia kuehnii E. J. Butler, causal agent of sugarcane orange rust(Virtudazo et al. 2001a). Uredinial lesions were orange and variable in size,hypophyllous, ellipsoidal to fusiform in shape, and distinctly lighter thanpustules of P. melanocephala that were present along with P. kuehnii.Urediniospores were mostly obovoid to pyriform, variable in size (29 to 41× 19 to 26 &#956;m), and moderately echinulate with mostly evenly distributedspines 2 to 4.5 &#956;m apart. Walls were orange-to-light cinnamon brown witha pronounced apical wall thickening as much as 7 &#956;m. Paraphyses, telia,and teliospores were not observed. Identification of the pathogen wasconfirmed by molecular studies. The internal transcribed spacer region(ITS1-5.8S-ITS2) of the rust infecting a nonidentified sugarcane cultivar(GenBank Accession No. KT261382) was sequenced. The sequence wasidentical to sequences of P. kuehnii and distinct from known sequences ofP. melanocephala (Virtudazo et al. 2001b). Orange rust, initially reported inthe Asia-Pacific region, has caused important losses in Australia in 2000.In the Western Hemisphere, it was first reported in Florida in 2007(Comstock et al. 2008). Since being reported in Brazil in 2009 (Barbasso et al.2010), annual surveys have been conducted in all three sugarcaneproduction regions of Argentina: Northern (Salta and Jujuy provinces);Northeast (Santa Fe and Misiones provinces); and Tucumán Province. Atpresent, the only focus of orange rust infection detected is the one inMisiones Province, 1,200 km from Tucumán, the main sugarcane-growingregion of the country. To our knowledge, this is the first report of P.kuehnii infecting sugarcane in Argentina. The possible economic impact oforange rust on sugarcane production in the country is still unknown, butconsidering its destructive potential, the disease may pose a serious threatto sugarcane production if environmental conditions favor itsestablishment during the early growth stages of the crop.","palabras_clave":"BEHAVIOUR AGAINST RUST|MARKER- ASSISTED SELECTION|PUCCINIA MELANOCEPHALA|PUCCINIA KUEHNII"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6277,"idioma_id":4,"titulo":"Peri-operative biology in primary breast cancer: selective targeting of vasopressin type 2 receptor using desmopressin as a novel therapeutic approach","resumen":"In breast cancer patients, surgical excision of the primary tumor is commonly the first step toward long-term control of disease progression. However, recent evidence suggests that surgery may also promote metastatic relapse through different deleterious mechanisms, including, but not limited to, tumor cell shedding, immunosuppression, neuroendocrine perturbations, increased production of growth factors, and proangiogenic signaling. In this regard, the perioperative timeframe represents an underexploited window of opportunity that could be targeted in order to modulate tumor-host interactions and minimize the risk of both local and metastatic recurrence.","palabras_clave":"Breast cancer|Surgery|Desmopressin|Metastasis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6278,"idioma_id":5,"titulo":"Dinámica de la innovación y estrategia de apropiación en una gran firma bio-farmacéutica argentina","resumen":"El artículo presenta el estudio de caso de Biosidus S.A., la más antigua e importante firma biofarmacéutica argentina. El análisis apunta a evidenciar cómo la estrategia de apropiación de los beneficios derivados de las innovaciones tecnológicas obtenidas por la firma se articula con su dinámica innovadora. Biosidus se caracteriza por tener una larga trayectoria innovadora, que seha apoyado en importantes capacidades internas y en fuertes vínculos con instituciones públicas de I+D con las que ha colaborado, lo que se ve reflejado en una amplia variedad de proyectos innovadores en el área de la biotecnología aplicada a salud humana. En ese marco, el objetivode este trabajo es doble. Por un lado, se analiza la estrategia de apropiación de la firmaevidenciando el uso que ella hace de diferentes mecanismos de apropiación existentes para proteger sus innovaciones tanto respecto a sus competidores como a sus partners de innovación. Por el otro, se relacionan las distintas variantes observables en tal estrategia con las características y la dinámica de la innovación desarrollada en los diferentes proyectos, en los que se identifican diferencias en lo relativo a objetivos, complejidad e importancia de la cooperación con centros públicos de I+D.","palabras_clave":"APROPIACION|INNOVACION|BIO-FARMACEUTICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6279,"idioma_id":4,"titulo":"Photoionization of planetary winds: case study HD 209458b","resumen":"Close-in hot Jupiters are exposed to a tremendous photon flux that ionizes the neutral escaping material from the planet leaving an observable imprint that makes them an interesting laboratory for testing theoretical models. In this work, we present 3D hydrodynamic simulations with radiation transfer calculations of a close-in exoplanet in a blow-off state. We calculate the Ly &#945; absorption and compare it with observations of HD 209458b and previous simplified model results. Our results show that the hydrodynamic interaction together with a proper calculation of the photoionization process are able to reproduce the main features of the observed Ly &#945; absorption, in particular at the blue-shifted wings of the line. We found that the ionizing stellar flux produce an almost linear effect on the amount of absorption in the wake. Varying the planetary mass-loss rate and the radiation flux, we were able to reproduce the 10 per cent absorption observed at -100 km s-1.","palabras_clave":"hydrodynamics|radiation mechanisms: general|methods: numerical|planets and satellites: individual: HD 209458b"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6280,"idioma_id":4,"titulo":"Effect of water availability and seed source on physical dormancy break of Vicia villosa ssp. villosa","resumen":"Physical dormancy (PY) has a crucial role in the control of the reseeding process of Vicia villosa Roth, a winter annual species cultivated for pasture and hay, naturalized in several semiarid temperate agroecosystems. As PY is considered a seed trait modulated under natural selection, populations from different origins should show different responses to environmental regulatory factors. The present study aimed to determine the effect of: (i) water availability on PY-break dynamics of a naturalized population from Argentina (ASC) under both laboratory and field conditions; (ii) the seed source on initial PY and dormancy release rate (wet storage at 20ºC) of ASC compared to 45 other populations of V. villosa including wild, naturalized, landraces and cultivars. Water availability increased PY loss rate under both storage and field conditions. ASC PY-break dynamics was adequately described by a Gompertz model with a lower thermal time requirement estimated for dormancy break under fluctuating soil water conditions compared to seeds buried inside impermeable bags. During the field burial experiment, a considerable proportion of seeds (~ 70%) became water permeable during the summer season after dispersal, and retained low levels of residual PY for soil seed bank replenishment. Improved populations (i.e. breeding cultivars) showed the lowest percentages of initial PY compared to landraces, naturalized and wild populations. Naturalized populations of Argentina showed similar initial PY compared to landraces, although PY release rate was lower in the former and might be attributed to local environmental selection. Wild types showed the lowest PY release rates.","palabras_clave":"PHYSICAL DORMANCY|SEMIARID ENVIRONMENT|THERMAL-TIME|V. VILLOSA GENOTYPES|WINTER ANNUAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6281,"idioma_id":4,"titulo":"Molecular Phylogeny and Morphological Analysis of Tetraglochin (Rosaceae: Rosoideae: Sanguisorbeae) and Recognition of the New Species T. andina","resumen":"Abstract?The genus Tetraglochin belongs to the family Rosaceae, subfamilyRosoideae, tribe Sanguisorbeae, and is distributed along the Andes from Peru tosouthern Argentina and central Chile. In order to test its monophyly and analyzerelationships among species of this genus and allied genera, we conductedmorphological studies and phylogenetic analyses using both nuclear (ITS) and cpDNA(trnL-F) sequences. As a result, Tetraglochin is supported as monophyletic, with thegenus Margyricarpus as its sister group. Tetraglochin acanthocarpa and T. caespitosaresulted basal to the other species of the genus, which were grouped in two mainsubclades: one of them primarily distributed along the Central Andes and including T.cristata, T. inermis and specimens previously classified as T. ameghinoi, this subcladewith coriaceous or papery not translucent hypanthium, and the other subclade, mainlydistributed along the Southern Andes, and comprising T. alata and the remainingspecimens of T. ameghinoi, this subclade characterized by their membranoustranslucent hypanthium. Based on results obtained from morphological studies,molecular phylogenies, and species delimitation analyses, a new species T. andina, isproposed, segregated from T. ameghinoi. A key to identify all species hereinrecognized, and a map containing geographical distribution of them are provided,together with a description and illustration of the new species.","palabras_clave":"Andes|Bayes factor|ITS|morphology|species delimitation|Tetraglochin andina|trnL-F"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6282,"idioma_id":4,"titulo":"Alcohol hangover: impairments in behavior and bioenergetics in Central Nervous System","resumen":"Alcohol hangover (AH) is defined as the temporary state after alcohol binge-like drinking, starting when EtOH is absent in plasma. Results from our laboratory have shown behavioral impairments and mitochondrial dysfunction in an experimental model of AH in mice. Our model consisted in a single i.p. injection of EtOH (3.8 g\/kg BW) or saline solution in male and female mice, sacrificing the animals 6 hours after injection. Motor and affective behavior together with mitochondrial function and free radical production were evaluated in brain cortex and cerebellum during AH. Results showed that hangover animals exhibited a significant reduction in neuromuscular coordination, motor strength and locomotion together with a loss of gait stability and walking deficiencies. Moreover, an increment in anxiety-like behavior together with fear-related phenotype and depression signs were observed. In relation to bioenergetics metabolism, AH induced a reduction in oxygen uptake, inhibition of respiratory complexes, changes in mitochondrial membrane permeability, decrease in transmembrane potential, increase in O2?- and H2O2 production and impairment in nitric oxide metabolism. All together our data suggest that the physiopathological state of AH involves behavioral impairments and mitochondrial dysfunction in mouse brain cortex and cerebellum showing the long lasting effects of acute EtOH exposure in CNS.","palabras_clave":"ALCOHOL HANGOVER|MOTOR PERFORMANCE|CEREBRAL CORTEX|FREE RADICALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6283,"idioma_id":4,"titulo":"Ketamine effect on intracellular and mitochondrial calcium mobilization.","resumen":"The suppressive effect of ketamine on intracellular calcium has been reported in a variety of cells although the mechanisms involved are not well understood. The aim of this work was to evaluate the effect of ketamine on mitochondrial Ca2+ accumulation and on cellular Ca2+ mobilization using FLUO4-AM and flow cytometry. The results showed that hippocampal mitochondria from ketamine injected animals presented a lower ability to retain calcium at concentrations higher than 20 &#61549;M, as compared with controls (saline injected animals). In addition, ketamine produced a significant decrease in KCl-induced intracellular Ca2+ influx in Vero cell cultures. According to these data, ketamine produces a clear inhibitory effect on cytosolic Ca2+ transport mechanisms, independent of the Ca2+ channel associated NMDA receptor.","palabras_clave":"KETAMINE|CALCIUM TRANSPORT|MITOCHONDRIA|HIPPOCAMPUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6284,"idioma_id":4,"titulo":"Frogs taste nice when there are few mice: Do dietary shifts in barn owls result from rapid farming intensification?","resumen":"Biodiversity ecosystem services in agroecosystems are negatively affected by farmland homogenisation due to intensive agriculture. The Pampas, an important worldwide region producing commodity crops, have been greatly homogenised with the expansion no-tillage and herbicide-tolerant transgenic soybeans since the 1990s. Here, we tested the hypothesis of that dietary changes in barn owls will be associated with the loss of semi-natural habitats derived from farming intensification. We characterised the dietary habits of western barn owls by analysing their pellets between two sampling periods (2004-2005 and 2010-2012). We also assessed the habitat loss due to cropping intensification through fencerow removal and pasture conversion to annual crops during the same period. We observed that barn owls shifted from eating mostly rodents in the first sampling period to eating a higher proportion of anurans in the second sampling period. Between sampling, rodent proportion in pellets decreased from 80% to 61.6%, while anuran proportion increased from 20% to 37.7%. A rapid farming intensification occurred on the farm between both sampling periods. Pastures were ploughed to grow annual crops. Thus, the annually cropped area increased by 60% from 2004 to 2012, while the area with pastures was reduced in about 80%. During the same period, nearly two-thirds of fences on the farm were removed to enlarge the cropped area. Our findings suggest that dietary habits of barn owls may shift to consume more anurans when rodent availability declines in association with the loss of semi-natural habitats, which resulted from rapid farming intensification on the studied farm. We suggest monitoring the population size and diet of barn owls for evaluating environmental changes produced in agroecosystems by farming intensification, as well as the adaptive responses of different rodent and anuran species to such changes.","palabras_clave":"AGRICULTURAL INTENSIFICATION|LANDSCAPE HOMOGENIZATION|BIODIVERSITY|TROPHIC RELATIONSHIPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6286,"idioma_id":5,"titulo":"Efecto de la morfología macroestructural sobre la resistencia a la corrosión de la aleación Al-12%Si en etanol","resumen":"Actualmente, la industria de los biocombustibles se encuentra en pleno crecimiento, y está situación promueve la búsqueda y la implementación de  nuevas tecnologías, tanto para la fabricación, como para la utilización de estos productos. El bioetanol es un combustible obtenido por fermentación de jugos ricos en azucares de origen vegetal, y representa uno de los más importantes sustitutos de los derivados de petróleo. El manejo de los mismos requiere el uso de materiales como el aluminio, por sus óptimas propiedades mecánicas y electroquímica, que además pueden ser mejoradas con el agregado de aleantes y variando su morfología macroestructural. En el presente trabajo de investigación se solidificaron probetas de aleación base aluminio Al-12%Si, y se determinó la resistencia a la corrosión de las diferentes estructuras de grano obtenidas (Columnar , Transición Columnar -Equiaxial (TCE) y Equiaxial ), expuestas a una solución compuesta por bioetanol de origen regional. Se obtuvieron resultados que evidencian diferencias entre las tres estructuras, tanto en la resistencia a la corrosión como en el comportamiento electroquímico.","palabras_clave":"Aluminio|Resistencia a la corrosión|Espectroscopia de Impedancia Electroquímica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6287,"idioma_id":4,"titulo":"Argentinean Society of Experimental Pharmacology: Brief history and main scientific contributions to the discipline","resumen":"Argentina Biomedical Science has been historically strong. The development of Human and Veterinary Pharmacology in our country as a pivotal discipline has been acknowledged worldwide because of the quality of its contributions. Argentinean Society of Experimental Pharmacology (SAFE) is a non- profit association whose research fields include Experimental and Clinical Pharmacology. SAFE main goals are described as follow (a) To meet active researchers for studying concerns regarding Experimental and Clinical Pharmacology (b) To launch an initiative for development of the discipline in mainly our country and other collaborative countries worldwide (c) To spread the pharmacological know-how obtained from different research teams (d) To strengthen relations between pharmacologists (e) To facilitate the presentation and discussion of scientific papers. This current article shows the SAFE´s more important scientific contribution to pharmacology through its former research scientists to the present.","palabras_clave":"ARGENTINA|FARMACOLOGIA|SAFE|CONTRIBUCIONES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6288,"idioma_id":4,"titulo":"Production of aromatic compounds in the heavy naphtha and light LCO ranges. Catalytic cracking of C10 naphthenic-aromatics and aromatics","resumen":"BACKGROUND: The conversions of bicyclic compounds, both a naphthenic-aromatic compound (tetralin) and an aromatic compound (naphthalene), as model reactants representative of the heavy gasoline and light cycle oil (LCO) cuts in fluid catalytic cracking (FCC), were studied to understand the formation of C10-C20 aromatic compounds in gasoline and middle distillates cuts, in view of their impact on the properties of the cuts. A commercial FCC catalyst was used in its fresh, hydrothermally de-aluminated and equilibrium forms, at 450 ºC in a fluidized bed CREC Riser Simulator reactor in the 2-8 s reaction time range.RESULTS: Products were C1-C14 hydrocarbons and coke. Based on the product distributions, reaction networks were proposed for both reactants. The reactions considered in the networks were hydrogen transfer, cracking, ring opening and contraction, alkylation and disproportionation.CONCLUSION: The load of zeolite in the catalysts and their acidities have the strongest influences on reaction selectivities. In the case of tetralin, the prevalent reaction is hydrogen transfer, which becomes more important as the catalysts are less active, the hydrocarbons with highest yields being C10 aromatics. Cracking reactions predominate in naphthalene conversion over all the catalysts, a fact which favors monoaromatic C9- hydrocarbons. These results can help to design new FCC catalysts with a better selectivity control.","palabras_clave":"CATALYSIS|ZEOLITES|POLYCYCLIC AROMATIC HYDROCARBONS (PAHs)|CATALYTIC PROCESSES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6289,"idioma_id":4,"titulo":"Efficient Estimation and Correction of Mismatch Errors in Time-interleaved ADCs","resumen":"We present a novel method for estimation and correction of mismatch errors in time-interleaved analog-to-digital converters. The estimation of the mismatch errors requires a training signal but is efficient, accurate and effective while keeping low the complexity of the associated algorithm, when compared to other work in the literature. The compensation strategy uses the estimated parameters for offset and gain mismatch error correction, and implements a low-complexity (similar as that of an FIR filter) Lagrange interpolation filter to correct errors due to timing mismatch, which depends on thedynamics of the input signal. The proposed compensation strategy achieves almost ideal behavior over a wide bandwidth, i.e. only 12% of oversampling is required for an almost complete cancellation of ditortion. The method has been tested under severe mismatch conditions (up to 10% timing mismatch and 5% gain and offset mismatch) showing an improvement of over 6 bits in the ENOB and 50 dB in the SFDR. In addition, when compared with the available techniques, no limitation on the number of channel converters are introduced since the compensator cancels effectively the distortion even in thepresence of large mismatches.","palabras_clave":"ANALOG-TO-DIGITAL CONVERTER|TIME-INTERLEAVED|MISMATCH ERROR|ESTIMATION - CORRECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6290,"idioma_id":4,"titulo":"Analysis of the h, H, A &#8594; &#964;&#956; decays induced from SUSY loops within the Mass Insertion Approximation","resumen":"In this paper we study the lepton favor violating decay channels of the neutral Higgs bosons of the Minimal Supersymmetric Standard Model into a lepton and an anti-lepton of different flavor. We work in the context of the most general flavor mixing scenario in the slepton sector, in contrast to the minimal flavor violation assumption more frequently used. Our analytic computation is a one-loop diagrammatic one, but in contrast to the full one-loop computation which is usually referred to the physical slepton mass basis, we use here instead the Mass Insertion Approximation (MIA) which uses the electroweak interaction slepton basis and treats perturbatively the mass insertions changing slepton flavor. By performing an expansion in powers of the external momenta in the relevant form factors, we will be able to separate explicitly in the analytic results the leading non-decoupling (constant at asymptotically large sparticle masses) and the next to leading decoupling contributions (decreasing with the sparticle masses). Our final aim is to provide a set of simple analytic formulas for the form factors and the associated effective vertices, that we think may be very useful for future phenomenological studies of the lepton flavor violating Higgs boson decays, and for their comparison with data. The accuracy of the numerical results obtained with the MIA are also analyzed and discussed here in comparison with the full one-loop results. Our most optimistic numerical estimates for the three neutral Higgs boson decays channels into &#964; and &#956; leptons, searching for their maximum rates that are allowed by present constraints from &#964; &#8594; &#956;&#947; data and beyond Standard Model Higgs boson searches at the LHC, are also included.","palabras_clave":"Higgs Physics|Supersymmetry Phenomenology|LHC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6291,"idioma_id":4,"titulo":"The appropriateness of contrast metric for reaction times","resumen":"We analyzed different contrast metrics to scale the stimulus strength for suprathreshold reaction times when it is modulated along an achromatic channel (L+M) and both chromatic channels L\/M and S\/(L+M) considering  increments and decrements along these axes. Reaction times were examined as a function of the Weber luminance contrast; spatial luminance ratio and, in terms of threshold units.The results show that when there is only luminance decreasing or increasing no such asymmetry between the two  is observed and our results indicate that both spatial luminance ratio, Weber luminance contrast or threshold units equate RT values. While,if the stimulus is modulated along an isoluminant plane the appropriate contrast is Weber (RMS) or SRL for stimuli modulated along L\/M axis and for stimuli modulated along S\/L+M,   showing an asymmetry between S-cone decrements and increments  in L\/M cone pathway. Threshold units are not appropriate, showing inconsistencies: the stimulus with chromatic direction equal to 90° appears as the most informative with a maximum gain. Even more so, the shared contrast gain grows as the size of the stimulus decreases. Aceptacion del articuloFrom: Jean-Pierre Jeannet Date: 2016-02-21 14:27 GMT-03:00Subject: Submitting publication material: Manuscript 8148 KRGTo: bodonell@herrera.unt.edu.arDear Beatriz Maria ODonellWe are informed that your paperThe appropriateness of contrast metric for reaction timeshas now been accepted for publication in Perception. Congratulations!Next, you will need to prepare and submit your final production materials within the next FIVE days (via PiMMS) to us at Pion Ltd, where your paper will be processed. Please note that at this stage we require separate hi-res (300 dpi) files for all figures in your paper (EPS, TIF, and PDF files only are accepted), along with a final version of the manuscript, formatted as per the style of Perception. Please also embed low-res figures within the manuscript, so that we can see where you would ideally like these placed. Please note, even if your paper does not contain any figures, we still require you to complete this stage.At the same time, if you previously indicated that your paper may be of interest to the general public, you will need to provide contact details for your University Press Office.Please click on the quick link below and follow the instructions to submit.http:\/\/submission.perceptionweb.com\/mms\/gopage.phtml?page=3dc8686230a34ea875bdb47e8251f2a7Best regards,Pion Ltd, publishers of Perception and i-Perception.PiMMS (Pion Manuscript Management System)-----------------------------------------------------------InstructionsPlease view the author guidelines at http:\/\/submission.perceptionweb.com\/supplement\/instructions\/pr\/authors.htmlIf you have any questions,please contact Gillian Porter at the editorial office, gillian.porter@bristol.ac.uk.","palabras_clave":"REACTION TIME|CONTRAST METRIC|THRESHOLD UNIT|SUPRATHRESHOLD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6292,"idioma_id":4,"titulo":"Pupil dynamics with periodic flashes: effect of age on mesopic adaptation","resumen":"The purpose of this study is to determine the pupillary dynamics with periodical flashes from a peripheral glare  source, in similar conditions to night driving, while focusing on dependence with age. We measured two groups of people: youth and adults. Maximum pupil size decreases due to periodic flashes. Latency does not present significant differences. The reduction of pupil size is greater for older adults. The presence of a peripheral and periodic glare source modifies the pupil size. This leads to a reduction of retinal illuminance, which is greater for older adults. © 2016 Optical Society of America","palabras_clave":"OPTICAL EFFECTS ON VISION|VISUAL ADAPTATION|DYNAMIC PUPIL|GLARE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6293,"idioma_id":5,"titulo":"Controversias acerca del diagnóstico de TDAH y la prescripción de metilfenidato en los debates sobre la medicalización en Argentina y Brasil","resumen":"Elartículo sistematiza y analiza resultados de investigaciones conducidas enArgentina y Brasil sobre el uso del metilfenidato, la regulación de suprescripción y venta, y el desarrollo de discusiones por diferentesinvestigadores y profesionales a cargo de la asistencia de personasdiagnosticadas por TDAH en ambos países. El artículo integra dos campos deinvestigación. En Argentina, se analizaron artículos periodísticos, datosnuméricos oficiales y de organizaciones profesionales, y entrevistassemiestructuradas individuales y grupales a profesionales de la salud,realizadas entre 2007 y 2011. En Brasil se analizaron artículos periodísticos yde revistas especializadas, se realizaron observaciones participantes yentrevistas a educadores y profesionales de salud. Las conclusiones sostienenque se han articulado organizaciones profesionales de ambos países, en unintento por promover el debate sobre la medicalización de la infancia y sudespliegue en la sociedad.","palabras_clave":"TDAH|Metilfenidato|Argentina|Brasil|medicalización"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6294,"idioma_id":4,"titulo":"Repeatability Evaluation of a Contrast Sensitivity System for Transfer to the Eye Clinic","resumen":"Abstract: The Contrast Sensitivity Function (CSF) is a valuable tool which can be used tocharacterize functional vision and also for the diagnosis and management of patients withdifferent eye diseases. In spite of its usefulness, the CSF is currently hardly ever used inclinical practice. The aim of this study was to validate the use of the system called FVC-100(Tecnovinc-UNT-CONICET, Argentina), which calculates the CSF, in order to transfer thisimportant tool to ophthalmological clinics. The validation was carried out through the designof a repeatability test and the subsequent analysis of the results. Furthermore, we evaluated theimpact of different factors influencing the repeatability of the measurements such as age andprevious training. The tests were based on the discrimination of sinusoidal gratings fordifferent spatial frequencies (1, 4 and 12 c\/°) in both eyes of 12 people, aged between 20 and70. The results show that the calculated values of SC of each subject have a high repeatabilityand are not dependent on age or training. These results allow us to conclude positivelyregarding the effectiveness of the FVC-100, and to validate its use in clinics for the calculationof the FSC as a standard measure of functional vision quality.","palabras_clave":"Contrast Sensitivity|Contrast Sensitivity FUNCTION|OPTICAL CLINIC|REPEATIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6295,"idioma_id":4,"titulo":"Photoinitiated Synthesis of Sulfides in Water","resumen":"Herein, we report a synthetic route to obtain aryl sulfides using inexpensive and non-toxic reactants and water as solvent, that avoids the use of catalysts or heating. The photoinduced reaction between soluble substrates and a series of thiols in alkaline aqueous medium produces the corresponding sulfides in moderate to good yields.","palabras_clave":"sulfides|water|photoinduced reaction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6296,"idioma_id":4,"titulo":"Time frequency analysis of neuronal populations with instantaneous resolution based on noise-assisted multivariate empirical mode decomposition","resumen":"BackgroundLinear analysis has classically provided powerful tools for understanding the behavior of neural populations, but the neuron responses to real-world stimulation are nonlinear under some conditions, and many neuronal components demonstrate strong nonlinear behavior. In spite of this, temporal and frequency dynamics of neural populations to sensory stimulation have been usually analyzed with linear approaches.New methodIn this paper, we propose the use of Noise-Assisted Multivariate Empirical Mode Decomposition (NA-MEMD), a data-driven template-free algorithm, plus the Hilbert Transform as a suitable tool for analyzing population oscillatory dynamics in a multi-dimensional space with instantaneous frequency (IF) resolution.ResultsThe proposed approach was able to extract oscillatory information of neurophysiological data of deep vibrissal nerve and visual cortex multiunit recordings that were not evidenced using linear approaches with fixed bases such as the Fourier analysis.Comparison with existing methodsTexture discrimination analysis performance was increased when Noise-Assisted Multivariate Empirical Mode plus Hilbert Transform was implemented, compared to linear techniques. Cortical oscillatory population activity was analyzed with precise Time-Frequency resolution. Similarly, NA-MEMD provided increased Time-Frequency resolution of cortical oscillatory population activity.ConclusionsNoise-Assisted Multivariate Empirical Mode Decomposition plus Hilbert Transform is an improved method to analyze neuronal population oscillatory dynamics overcoming linear and stationary assumptions of classical methods.","palabras_clave":"NA-MEMD|nonlinear analysis|non-stationary analysis|neuronal population"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6297,"idioma_id":5,"titulo":"La comunicación de la administración pública en las redes sociales: los casos de las ciudades de Buenos Aires y Bolonia","resumen":"El artículo profundiza en los modelos de gestión, la propuesta comunicativa y las estrategias de las administraciones públicas para involucrarse con los ciudadanos en las redes sociales, a más de un lustro de la incorporación de los recursos de la web 2.0 en la comunicación oficial de las instituciones de gobierno, a través del estudio de caso comparado de dos entes locales urbanos, Buenos Aires (Argentina) y Bolonia (Italia). Desde una perspectiva centrada en los paradigmas opuestos ?conversacional-unidireccional? y ?abierto-cerrado?, la investigación recoge los criterios de publicación utilizados por los responsables de las cuentas oficiales de cada una de las ciudades, así como también la gramática de los mensajes y los sujetos y temas que se conectan en ellos. A su vez, analiza los contenidos de las publicaciones de las cuentas institucionales de ambas ciudades en Twitter, Facebook y YouTube durante un período de seis semanas entre los meses de octubre y diciembre de 2014, clasificándolas en categorías elaboradas con el fin de mostrar el nivel de reconocimiento y empoderamiento de los 1 Universidad Católica Argentina, Argentina. mariano_ure@uca.edu.ar Palabra Clave - ISSN: 0122-8285 - Vol. 19 No. 1 - Marzo de 2016. 240-270 241 usuarios en calidad de ciudadanos. La diversidad de las propuestas comunicativas observada entre los casos estudiados pone en discusión la definición acerca de las ?buenas prácticas sociales?, y plantea la necesidad de ahondar en los márgenes reales que poseen las administraciones públicas para el aprovechamiento de las posibilidades de interacción que ofrecen las plataformas interactivas.","palabras_clave":"ADMINISTRACIÓN PÚBLICA|REDES SOCIALES|CIUDADANOS|CONTRATO COMUNICATIVO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6298,"idioma_id":4,"titulo":"Functional specificity of rat vibrissal primary afferents","resumen":"In this study, we propose to analyze the peripheral vibrissal system specificity through its neuronal responses. Receiver operating characteristics (ROC) curve analyses were used, which required the implementation of a binary classifier (artificial neural network) trained to identify the applied stimulus. The training phase consisted of the observation of a predetermined amount of vibrissal sweeps on two surfaces of different texture and similar roughness. Our results suggest that the specificity of the peripheral vibrissal system easily permits the discrimination between perceived stimuli, quantified through neuronal responses, and that it can be evaluated through an ROC curve analysis. We found that such specificity makes a linear binary classifier capable of detecting differences between stimuli with five sweeps at most.","palabras_clave":"PERIPHERAL AFFERENT ACTIVITY|ROC ANALYSIS|VIBRISSAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6300,"idioma_id":4,"titulo":"Mechanisms of innate immune activation by gluten peptide p31-43 in mice","resumen":"Celiac disease (CD) is an immune-mediated enteropathy triggered by gluten in genetically susceptible individuals. Innate immunity contributes to the pathogenesis of CD, but the mechanisms remain poorly understood. Although previous in vitro work suggests that gliadin peptide p31-43 acts as an innate immune trigger, the underlying pathways are unclear and have not been explored in vivo. Here we show that intraluminal delivery of p31-43 induces morphological changes in the small intestinal mucosa of normal mice consistent with those seen in CD, including increased cell death and expression of inflammatory mediators. The effects of p31-43 were dependent on MyD88 and Type I IFNs, but not TLR4, and were enhanced by co-administration of the TLR3 agonist poly I:C. Together these results indicate that gliadin peptide p31-43 activates the innate immune pathways in vivo, such as IFN-dependent inflammation, relevant to CD. Our findings also suggest a common mechanism for the potential interaction between dietary gluten and viral infections in the pathogenesis of CD.","palabras_clave":"experimental enteropathy|celiac disease|innate immunity|inflammation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6301,"idioma_id":4,"titulo":"Flooding risks and housing markets: a spatial hedonic analysis for La Plata City","resumen":"On 2 April 2013 a major flood struck the City of La Plata, Argentina, killing 89 people and displacing thousands of others. That event, the worst flood the city has suffered in the past 100 years, prompted plans for a new hydraulic infrastructure. Although such an investment is necessary, little is known about its benefits. This paper intends to shed some light on this issue by estimating the willingness to pay to avoid the risk of experiencing a flooding event. For this purpose, we have taken thousands of real estate prices in the La Plata Metropolitan Area and combined them with a high-resolution flood risks map to estimate spatial hedonic price models. The results show significant price discounting for properties in flood-prone areas.","palabras_clave":"flooding|hedonic models|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6302,"idioma_id":5,"titulo":"La teoría de la mente como proceso mediador del funcionamiento social. Evaluación en el Síndrome de Turner","resumen":"La teoría de la mente es la capacidad de atribuir mente a otros. El Síndrome de Turner es un trastorno cromosómico caracterizado por un desarrollo atípico de ciertas áreas cerebrales implicadas en procesos cognitivos sociales, existiendo evidencia empírica del déficit presente en su funcionamiento social. Objetivo: caracterizar la Teoría de la Mente en mujeres con diagnóstico de Síndrome de Turner. Muestra: 20 mujeres con diagnóstico de Síndrome de Turner y sus controles. Instrumento: comunicaciones metafóricas e historias extrañas de Happé. Resultados y discusión: se hallaron diferencias estadísticamente significativas entre el grupo clínico y control en varias subdimensiones de las historias extrañas y en el puntaje total, con una magnitud del efecto alta. Las dificultades sociales presentes en las mujeres con diagnóstico de Síndrome de Turner podrían vincularse con un déficit en Teoría de la Mente respecto del reconocimiento de sentidos no literales, implicando un desfasaje en los aspectos pragmáticos de la comunicación social.","palabras_clave":"SÍNDROME DE TURNER|DISFUNCIÓN SOCIAL|TEORÍA DE LA MENTE|SENTIDOS NO LITERALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6304,"idioma_id":5,"titulo":"CARACTERIZACIÓN FITOLÍTICA DE LA FORMACIÓN ARROYO  FELICIANO (PLEISTOCENO SUPERIOR) EN LA LOCALIDAD PASO DUARTE,  ENTRE RÍOS, ARGENTINA","resumen":"Abstract. PHYTOLITH CHARACTERIZATION FROM THE ARROYO FELICIANO FORMATION (UPPER PLEISTOCENE), PASO DUARTE LOCALITY,ENTRE RÍOS PROVINCE, ARGENTINA. In this contribution a phytolith assemblage of the Arroyo Feliciano Formation (Upper Pleistocene), PasoDuarte locality (Entre Ríos Province), upper Río Gualeguay Basin, was studied. The scarce faunal paleontological record present in the formationhave been tentatively correlated with the Bonaerian?Lujanian Stage\/Age from Buenos Aires Province, Argentina. The paleobotanical recordwas studied in the last three years, and is composed by fossil woods that characterize warm and humid to semi-arid environments. The phytolithassemblages of Paso Duarte locality show a composition of elements that allows us to distinguish three sectors along the profile. Theanalysis of the phytolith assemblages suggests the presence of grasses palaeocommunities, principally dominated by meso-megathermicelements that grew under warm and humid climatic conditions.Key words. Quaternary. Gualeguay River Basin. Paleoclimate. Paleoenvironment. Silicophytoliths","palabras_clave":"Cuaternario. |Cuenca del río Gualeguay. |Paleoclima. Paleoambiente. |Silicofitolitos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6305,"idioma_id":4,"titulo":"Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD","resumen":"During the cattle breeding, the animals are exposed to stressful conditions (confinement, competition, extreme temperatures, pathogens); these have been associated to the increase of reactive oxygen species that attack cell membrane unsaturated lipids and other biomolecules and thus, inducing oxidation. This oxidative stress increases the incidence of degenerative diseases and decreases productivity. It has been proposed that the supplementation of the animal diet with antioxidants decreases the oxidative stress. Malondialdehyde (MDA) is an advanced oxidation product and a recognized oxidative stress biomarker. The aims of this work were to validate a methodology to determine MDA in goat plasma by HPLC-DAD and its assay in an animal feeding experiment. The MDA level wasmeasured after condensation reaction with thiobarbituric acid (TBA). Free MDA (FMDA) was directly quantified after TBA condensation; while, Protein-bound MDA (PBMDA) needed to be released by an alkaline hydrolysisstep and followed by TBA condensation. The MDA detection and quantitation limits were 0.034 &#956;M and 0.086 &#956;M, respectively. The linear range was 0.086?9.1 &#956;M (R2= 0.9998). The accuracy was 100.5% to FMDA and 102.4% to PBMDA. The precision values intra and interday were 3.7 and 3.9%, respectively, for FMDA; and 3.8 and4.1%, respectively, for PBMDA. Samples were analyzed in triplicate to obtain the figures of merit. The MDA level was quantified in plasma samples of 19 goats (Capra hircus) randomly distributed in three groups and fed with isocaloricand isoproteic diets: control, and supplemented with 12.5% d.b. of native woody species as polyphenol sources. TheMDA plasma levels were statistically different among treatments in regard to the animal diet. A protector effect of the supplementation against oxidative stress was observed. The proposed methodology was selective, sensitive, reproducible, and suitable to evaluate MDA in goat plasma as oxidative stress biomarker.","palabras_clave":"MALONDIALDEHYDE|OXIDATION|STRESS|PLASMA|ANTIOXIDANT|DIET"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6306,"idioma_id":4,"titulo":"Nesting biology and associated insect enemies of the exotic leaf cutter bee Megachile (Eutricharaea) concinna (Hymenoptera: Megachilidae) in Argentina","resumen":"The nesting biology of the exotic bee Megachile (Eutricharaea) concinna Smith, 1879 and its negative interactions with other insects were studied. Trap nests made of wood were located in an urban environment in Buenos Aires province, Argentina, and surveyed during 26 months. The lifecycle and the structure of the nest of M. concinna are described. We also report six interactions between this bee and native and exotic parasitoids and cleptoparasites. The species Baryscapus megachilidis is recorded for the first time in Argentina.","palabras_clave":"LEAFCUTTER BEE|TRAP NESTS|PARASITOIDS|ALIEN BEES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6307,"idioma_id":4,"titulo":"Dengue Virus Genome Uncoating Requires Ubiquitination","resumen":"The process of genome release or uncoating after viral entry is one of the least studied steps in the flavivirus life cycles. Flaviviruses are mainly arthropod-borne viruses including emerging and re-emerging pathogens such as dengue, Zika and West Nile viruses. Currently, dengue is one of the most significant human viral pathogens transmitted by mosquitoes, responsible for about 390 million infections every year around the world. Here, we examined for the first time molecular aspects of dengue virus genome uncoating. We followed the fate of the capsid protein and RNA genome early during infection and found that capsid is degraded after viral internalization by the host ubiquitin-proteasome system. However, proteasome activity and capsid degradation were not necessary to free the genome for initial viral translation. Unexpectedly, genome uncoating was blocked by inhibiting ubiquitination. Using different assays to bypass entry and evaluate the first rounds of viral translation, a narrow window of time during infection that requires ubiquitination but not proteasome activity was identified. In this regard, ubiquitin E1 activating enzyme inhibition was sufficient to stabilize the incoming viral genome in the cytoplasm of infected cells, causing its retention either in endosomes or nucleocapsids. Our studies support a model in which dengue virus genome uncoating requires a non-degradative ubiquitination step, providing new insights on this crucial but understudied viral process.","palabras_clave":"Dengue|Ubiquitin|Uncoating"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6308,"idioma_id":5,"titulo":"Determinación del tamaño y volumen renal en recién nacidos considerados normales según criterios de la OMS mediante ecografía. Estudio descriptivo, observacional y transversal con revisión de la bibliografía","resumen":"Introducción: La masa renal y el número deglomérulos se correlacionan significativamenteen niños menores de tres meses así como enadultos normales, pero ambos solo son medibles?ex vivo?. Por otro lado, el volumen renal (VR),también proporcional a la masa renal, puede serobtenido por ecografía, como un sustituto para laestimación del número de glomérulos ?in vivo?.El VR se calcula mediante una fórmula que incluyela medición de tres diámetros renales: longitudinal(DL), antero-posterior (DAP) y transverso(DT). Objetivos: Determinar los diámetros DT,DL, DAP y el volumen renal; y establecer la relaciónde estos diámetros con las medidas antropométricasen neonatos (dentro de las 48 hs. denacidos), considerados normales según criteriosde la OMS7, de la ciudad de Resistencia, provinciadel Chaco, República Argentina. Material ymétodos: Se realizó un estudio descriptivo observacional,de corte transversal, con muestreono probabilístico de 27 neonatos de ambos sexosdefinidos como sanos tanto en las condicionesperinatales como del neonato. Los estudios eco-ARTÍCULO ORIGINALDETERMINACIÓN DEL TAMAÑO Y VOLUMEN RENAL EN RECIÉNNACIDOS CONSIDERADOS NORMALES SEGÚN CRITERIOS DE LAOMS MEDIANTE ECOGRAFÍA. ESTUDIO DESCRIPTIVO, OBSERVACIONALY TRANSVERSAL CON REVISIÓN DE LA BIBLIOGRAFÍADETERMINATION OF RENAL LENGTH AND VOLUME THROUGH ULTRASOUNDSCANNING IN NEWBORN INFANTS CONSIDERED NORMAL ACCORDINGTO WHO CRITERIA. A DESCRIPTIVE, OBSERVATIONAL, CROSS-SECTIONALSTUDY AND LITERATURE REVIEWMaría Eugenia Victoria Bianchi 1, Germán Darío Lopez 2, Ana María Tauguinas 1, Gustavo Velasco 1,Daniel Forlino 21) Fundación Renal del Nordeste Argentino, Resistencia, Chaco, Argentina2) Facultad de Medicina, Universidad Nacional del Nordeste, Corrientes, ArgentinaRev Nefrol Dial Traspl. 2016; 36 (4): 211-21gráficos fueron realizados por un solo especialistaen diagnóstico por imágenes, estando aún internadosla madre y el recién nacido (RN). Resultados:El DL fue de 48,3 y 48,1 mm para el RD ypara el RI respectivamente. El volumen renal total(VRT) fue de 23,3 ± 4,8 ml. Las medias halladasfueron 4,2 cm x 2,3 cm x 2,2 cm. Sólo en el DT(tanto en RI y RD) se encontró correlación conpeso, talla y superficie corporal (SC) (p < 0,01).Conclusiones: Los diámetros y el VR determinadosse encuentran en un rango intermedio a losdescritos en la bibliografía. El DT se correlacionócon peso, talla y SC. La forma de los riñonesde neonatos es diferente a la de riñones de niñosmayores o adultos, debiéndose comprobar si paraeste grupo etáreo se debe adoptar otra fórmula,la del cilindro elíptico en lugar de la fórmula delelipsoide o de Dinkel.","palabras_clave":"ECOGRAFIA RENAL|ULTRASONOGRAFIA|NEONATOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6309,"idioma_id":4,"titulo":"Electrochemistry of Tris(1,10-phenanthroline)iron(II) inside a polymeric hydrogel. Coupled chemical reactions and migration effects","resumen":"The retention, release, and detection of metallic complexes in polymeric hydrogels are of interest in drug delivery, analytical chemistry, and water remediation. The electrochemistry of the redox complexes inside the hydrogel could be affected by the viscoelastic properties of the gel, local ionic force and pH, and interactions (e.g., hydrophobic) between the complex and the polymer chains. In this work, it is shown that a simple setup, consisting of a disk electrode pressed on the hydrogel, allows to perform electrochemistry of a redox couple: Tris(1,10-phenanthroline)iron(II) (Fe(phen)3 2+) inside a hydrogel matrix. The behavior is compared with the same couple in solution, and it is found that the electrochemical properties of the redox couple are strongly affected by the presence of the hydrogel matrix. The cyclic voltammogram of the hydrogel loaded with complex shows a response, which suggests electrochemical-chemical mechanism. The chemical step is likely linked to a catalytic oxidation of free hydrated Fe2+ ions present inside the hydrogel together with the redox complex. Since Fe2+ ions have small charge transfer constants on the glassy carbon electrodes, only the catalytic current is observed. Indeed, when excess ligand (phenanthroline) is absorbed inside the hydrogel, the measured cyclic voltammograms show a single reversible oxidation\/reduction step. It seems that the complexation equilibrium shifts toward the complex, making the free iron concentration negligible. Accordingly, the cyclic voltammetry shape and peak potential difference agree with a reversible oxidation\/reduction. Additionally, the peak currents of the cyclic voltammograms show a linear dependence with the square root of time, as predicted by a Randles-Sevcik equation. However, the measured currents are smaller than the simulated ones. The differences are in agreement with simulations of the cyclic voltammograms where the migration of the redox species is considered. Chronoamperometry is used to measure the mass transport of redox species inside the hydrogel. It is found that the current transients still obey Cottrell&rsquo;s equation, but the diffusion coefficients obtained from the slopes of Cottrell&rsquo;s plots have to be corrected for migration effects. The effective diffusion coefficient of Fe(phen)3 2+ measured inside the hydrogel (DRed-hydrogel&nbsp;=&nbsp;5.5 (&plusmn;0.5)&nbsp;&times;&nbsp;10&minus;8&nbsp;cm2&nbsp;s&minus;1) is ca. 80 times smaller than the one measured in solution (DRed-solution&nbsp;=&nbsp;4.4 (&plusmn;0.5)&nbsp;&times;&nbsp;10&minus;6&nbsp;cm2&nbsp;s&minus;1). The simple setup has a true semi-infinite boundary condition, which allows characterizing the hydrogel in the same condition as the bulk material and easily changing both the redox species and the hydrogel structure.","palabras_clave":"HYDROGEL|IRON|PARTITION|PHENANTHROLINE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6310,"idioma_id":4,"titulo":"Designing robust watermark barcodes for multiplex long-read sequencing","resumen":"Motivation: To attain acceptable sample misassignment rates, current approaches to multiplex single-molecule real-time sequencing require upstream quality improvement, which is obtained from multiplepasses over the sequenced insert and significantly reduces the effective read length. In order to fullyexploit the raw read length on multiplex applications, robust barcodes capable of dealing with the fullsingle-pass error rates are needed.Results: We present a method for designing sequencing barcodes that can withstand a large number ofinsertion, deletion and substitution errors and are suitable for use in multiplex single-molecule real-timesequencing. The manuscript focuses on the design of barcodes for full-length single-pass reads, impairedby challenging error rates in the order of 11%. The proposed barcodes can multiplex hundreds orthousands of samples while achieving sample misassignment probabilities as low as 10&#1048576;7 under theabove conditions, and are designed to be compatible with chemical constraints imposed by the sequencingprocess.Availability: Software tools for constructing watermark barcode sets and demultiplexing barcodedreads, together with example sets of barcodes and synthetic barcoded reads, are freely available atwww.cifasis-conicet.gov.ar\/ezpeleta\/NS-watermark.","palabras_clave":"NO CORRESPONDE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6311,"idioma_id":5,"titulo":"Caracterización ambiental de la zona periurbana de Villa María, Córdoba para la conformación de indicadores ambientales","resumen":"La localidad de Villa María, Córdoba, cuenta con el 59.48% desu superficie como áreas periurbanas. El tipo de uso que se designaen este espacio es de suma importancia no solo porquerepresenta más de la mitad de su superficie sino además, pordesarrollarse actividades que no se encuentran en la zona urbana.La generación de indicadores ambientales, que permitan conocerel estado, la presión y la respuesta que se sucede en el ambiente,son de gran relevancia para poder prevenir riesgos ambientales yobtener un ordenamiento del territorio optimizando los servicioambientales y generando el menor impacto.La caracterización ambiental de la zona periurbana de Villa Maríapermitió conocer el espacio, las diversas actividades que en él sedesarrollan y a partir de allí establecer los indicadores más apropiadospara cada tipo de actividad. Proyecto «Calidad ambientalen la región periurbana de Villa María, Córdoba», financiado porla Universidad Nacional de Villa María.","palabras_clave":"PERIURBANO|VILLA MARIA|CALIDAD AMBIENTAL |INDICADORES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6312,"idioma_id":4,"titulo":"Prevalence, Patterns, and Correlates of Physical Activity Among the Adult Population in Latin America: Cross-Sectional Results from the CESCAS I Study","resumen":"Background: Few data are available on population level regarding domain-specific correlates of physicalactivity (PA) in Latin America.Objective: The aim of this study was to examine the relationships among PA patterns and theirmain sociodemographic determinants and cardiovascular risk factors in the Southern Cone of LatinAmerica.Methods: CESCAS I is a population-based prospective cohort study with a 4-stage stratified sampling of ageneral population of 7,524 adults aged 35 to 74 years from 4 midsized cities in Argentina, Chile, andUruguay. PA was assessed using the transcultural adaptation of the International Physical Activity Questionnairelong form. The questionnaire asked about frequency (days per week) and duration (minutes per day)of moderate and vigorous intensity activities in 3 different domains: work, leisure time, and active transportation(walking and bicycling). PA levels of 600 metabolic equivalent tasks (MET) minutes per week wasconsidered sufficiently active. Odds ratios for associations of sufficiently active status with sociodemographicdeterminants and cardiovascular risk factors were obtained using multivariable-adjusted logistic regressionmodels.Conclusions: Almost 65%) of the participants reported 600 MET minutes per week. The lowest prevalence ofsufficiently active individuals was seen in Temuco, Chile (58.0%), among women (58.7%), older individuals(55.4%), those with higher educational level (61.6%), and homemakers (53.4%). Approximately 22.8% of thepopulation reported no PA. In multivariable analysis, PA levels were lower among women, individuals whowere older, obese, university educated, with clerical work, retired\/unemployed or homemakers, and thosewith physical limitations. Future interventions to increase PA levels in the Southern Cone of Latin Americamust take into account disparities by gender and socioeconomic status. The promotion of PA during leisuretime in women?unemployed and homemakers?and of active transportation for those performing office orclerical work should be a priority in this population.","palabras_clave":"PREVALENCE|PHYSICAL|ACTIVITY|LATINAMERICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6313,"idioma_id":4,"titulo":"The relay network of Geobacter biofilms","resumen":"While actual models explaining electron conduction in electricity producing biofilms have evolved separately to apparent irreconcilable conceptual positions, finding cytochrome complexes in the external matrix of Geobacter biofilms supports the proposal of a new functional model, that takes fundamental elements from confronting theories. In this model electrons expelled by cells are conducted to the collecting electrode along a network of supramolecular cytochrome arrangements interconnected by semiconducting pilus fibres that provide equipotential conditions within physically distant points. This arrangement resembles, from our point of view, a relay network for Geobacter biofilm, which allows a concerted physiological response of the entire population to any local redox change.","palabras_clave":"REDOX CONDUCTION|BIOFILMS|ENERGY|WASTEWATER TREATMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6314,"idioma_id":4,"titulo":"Impact of Magnetite Iron Oxide nanoparticles on wheat (Triticum aestivum L.) development: Evaluation of oxidative damage","resumen":"Interest on the environmental impact of engineered nanomaterials has rapidly increased over the pastyears because it is expected that these materials will eventually be released into the environment. In thiswork, physiological effects and possible cell internalization of citric acid coated-Fe3O4 nanoparticles(5, 10, 15, 20 mg L1) on wheat (Triticum aestivum L.) plants grown five days under hydroponic conditionswere evaluated. Visualization of root sections by transmission electron microscopy showed that Fe3O4nanoparticles entered the root through the apoplastic route and were then detected in the root epidermalcell walls. Moreover, strong magnetic signals detected by vibrating sample magnetometry (VSM) and ahuge increment in the Fe content (8,07 and 2,01 mg g1 DW for NP20 and C-NP20 treatmentsrespectively) were observed in wheat roots treated with Fe3O4 nanoparticles. However, no superparamagneticsignal was detected in the aerial part which indicated that magnetite nanoparticles werenot translocated by vascular tissues in wheat plants in the experimental conditions of this study.Moreover, Fe3O4 nanoparticles did not affect the germination rate, the chlorophyll content, and theplant growth, and they did not produce lipid peroxidation, nor alter O2 or H2O2 accumulation respect tocontrol plants. Furthermore, electrolyte release and cell death percentage were not modified bynanoparticle treatment. The antioxidant enzyme activities of NP treated plants significantly increased inboth the root and the aerial part respect to the controls, showing a response leading to prevent oxidativedamage. These preliminary results show that these Fe3O4 nanoparticles are not phytotoxic, suggestingthat they could potentially be useful for the design of new products for agricultural use.","palabras_clave":"Nanopartículas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6315,"idioma_id":4,"titulo":"The Acoustic Properties of Suspended Sediment in Large Rivers: Consequences on ADCP Methods Applicability","resumen":"The use of echo-levels fromAcoustic Doppler Current Profiler (ADCP) recordings has become more and morecommon for estimating suspended bed-material and wash loads in rivers over thelast decade. Empirical, semi-empirical and physical-based acoustic methods havebeen applied in different case studies, which provided relationships betweenscattering particles features derived from samples (i.e., concentration andgrain size) and corresponding backscattering strength and sound attenuation.These methods entail different assumptions regarding sediment heterogeneity inthe ensonified volume (e.g., particle size distribution (PSD) and spatialconcentration gradient). Our work was to compare acoustic backscatter andattenuation properties of suspended sediments, sampled in the rivers Parana andDanube that represented rather different hydro-sedimentological conditionsduring the surveys. The Parana represents a large sandy river, characterizedthrough a huge watershed and the typical bimodal PSD of sediment in suspension,while the Danube represents in the investigated reach an exposed sand-gravelbed and clay-silt particles transported in the water column in suspension. Sandand clay-silt concentrations clearly dominate the analyzed backscattering strengthin the rivers Parana and Danube, respectively, with an effect of PSD level ofsorting in the latter case. This comparison clarifies the extent of assumptionsmade, eventually advising on the actual possibility of applying certain ADCPmethods, depending on the expected concentration gradients and PSD of suspendedsediment to be investigated.","palabras_clave":"BACKSCATTER|SOUND-ATTENUATION|SUSPENDED-LOAD|PARTICLE-DISTRIBUTION|ADCP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6316,"idioma_id":4,"titulo":"Towards a set of agrosystem-specific cropland mapping methods to address the global cropland diversity","resumen":"Accurate cropland information is of paramount importance forcrop monitoring. This study compares five existing cropland mappingmethodologies over five contrasting Joint Experiment forCrop Assessment and Monitoring (JECAM) sites of medium tolarge average field size using the time series of 7-day 250 mModerate Resolution Imaging Spectroradiometer (MODIS) meancomposites (red and near-infrared channels). Different strategieswere devised to assess the accuracy of the classification methods:confusion matrices and derived accuracy indicators with and withoutequalizing class proportions, assessing the pairwise differenceerror rates and accounting for the spatial resolution bias. Therobustness of the accuracy with respect to a reduction of thequantity of calibration data available was also assessed by a bootstrapapproach in which the amount of training data was systematicallyreduced. Methods reached overall accuracies ranging from85% to 95%, which demonstrates the ability of 250 m imagery toresolve fields down to 20 ha. Despite significantly different errorrates, the site effect was found to persistently dominate themethod effect. This was confirmed even after removing theshare of the classification due to the spatial resolution of thesatellite data (from 10% to 30%). This underlines the effect ofother agrosystems characteristics such as cloudiness, crop diversity,and calendar on the ability to perform accurately. All methodshave potential for large area cropland mapping as they providedaccurate results with 20% of the calibration data, e.g. 2% of thestudy area in Ukraine. To better address the global croplanddiversity, results advocate movement towards a set of croplandclassification methods that could be applied regionally accordingto their respective performance in specific landscapes.","palabras_clave":"Classification|MODIS|Intercomparisson|JECAM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6317,"idioma_id":4,"titulo":"Modulating effects of orally supplied Euglena gracilis on the physiological responses of the freshwater mussel Diplodon chilensis, exposed to sewage water pollution in a Patagonian river (Argentina)","resumen":"In order to test if orally supplied Euglena sp. cells modulate the physiological status of bivalves during bioremediation procedures, we evaluated the effect of Euglena gracilis diet on the immune response, oxidative balance and metabolic condition of Diplodon chilensis exposed to sewage water pollution. Mussels were fed for 90 days with E. gracilis (EG) or Scenedesmus vacuolatus (SV, control diet), and then exposed for 10 days at three sites along the Pocahullo river basin: 1) an unpolluted site, upstream of the city (control, C); 2) upstream (UpS) and 3) downstream (DoS) from the main tertiary-treated sewage discharge, in the city of San Martín de los Andes, Northwest Patagonia, Argentina. Our results show that the total hemocyte number decreases while pollution load increases along the river course for both, EG and SV mussels. Phagocytic activity is higher in EG mussels than in SV ones under all conditions. Reactive oxygen species (ROS) production in hemocytes increases with the increase in the pollution load, being significantly higher for EG mussels than for SV ones at DoS; no changes are observed for total oxyradical scavenging capacity (TOSC). Hemocytes? viability is increased for E. gracilis diet at C and remains unchanged in this group of mussels when exposed at the polluted sites. Lysosomal membrane stability is higher in EG mussels than in SV ones for all conditions, although it is decreased at polluted sites compared with that at C. Antioxidant (catalase) and detoxifying (gluthatione S-transferase) defenses are  generally lower in gills and digestive gland of EG mussels than in SV ones. Lipid peroxidation (TBARS) is evident in gills of EG mussels at C, and in digestive gland of the same group, at all the sites. Gill mass factor (GF) is affected by the E. gracilis diet; it is increased at C and decreased at polluted sites when compared with that of SV ones. Digestive gland mass factor (DGF) is higher in EG mussels than in SV ones. In D. chilensis, continuous and long term feeding with E. gracilis cells favors immune response and reduces the damage caused by sewage pollution exposure on hemocytes. Nevertheless, diet and transplantation procedures may produce negative effects on the oxidative balance of gills and digestive gland and should be taken into account for bioremediation strategies.","palabras_clave":"BIVALVE|PARAMYLON |IMMUNE RESPONSE|OXIDAIVE STRESS|METABOLIC CONDITION|URBAN POLLUTION|BIOREMEDIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6318,"idioma_id":4,"titulo":"Ultrasound-assisted pretreatment for multielement determination in maize seed samples by microwave plasma atomic emission spectrometry (MPAES)","resumen":"In this work, a rapid and simple ultrasound-assisted pretreatment for maize samples is proposed. Also, the performance of microwave plasma atomic emission spectrometry (MPAES) for the analysis of Ag, Al, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Na, Ni, Pb, Tl, V and Zn in maize seeds, was evaluated. Instrumental parameters optimization, as well as validation and application of the MPAES are presented. The obtained limits of detection were ranged between 0.7 &#956;g g-1 (for Mo and Fe) and 4.3 &#956;g g-1 (for Ca). Validation assays were carried out by standard addition and internal standard, showing good recovery performances, over 96% in all cases. The developed method was applied to trace elements determination of Argentinean maize samples, improving the productivity performance due the reducing costs - by the use of atmospheric nitrogen as plasma source for MPAES - and time analysis, which could be trustworthy for routine analysis, quality control and traceability of maize.","palabras_clave":"Maize|Ultrasound-assisted|Sample-pretreatment|Multielemental-analysis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6319,"idioma_id":4,"titulo":"Continuous method to determine the trypsin inhibitor activity in soybean flour","resumen":"The determination of trypsin inhibitor (TI) activity is of importance to evaluate the nutritional value of soybean flours. An analytical method, which involves a continuous spectrophotometric rate determination for trypsin activity against the substrate N-benzoyl-DL-arginine p-nitroanilide, is proposed as an alternative to the standard discontinuous assay. Stopping the reaction with acetic acid and a centrifugation\/filtration step to decrease turbidity are not required, thus reducing costs and time-wasting. The TI activity of different flour samples, determined by both assays, demonstrated to be statistically comparable, irrespective of the TI concentration level. The coefficients of variation of the novel method did not exceed 8 % at any concentration level. The curves of progress reaction showed a non-linear behavior in samples without TI. A reduction of incubation times from 10 min to 2 min increased the method sensitivity and extended its linear range. A more economical, faster and simpler TI activity assay was developed.","palabras_clave":"SOYBEAN FLOUR |ANTINUTRITIONAL FACTORS|TRYPSIN INHIBITOR ACTIVITY|ANALYTICAL METHOD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6322,"idioma_id":5,"titulo":"Cinética de secado de cucurbita moschata, utilizando un secador solar indirecto","resumen":"En este trabajo se presentan los resultados del secado solar de Cucurbita moschata, obtenidos en Santiago del Estero, Argentina, en un equipo diseñado para el secado solar de productos alimenticios principalmente agrícolas. El rango de temperatura utilizado en dicho proceso se encuentra entre 40 70 °C. El diseño y uso de estos tipos de dispositivos de secado solar es una alternativa a la utilización de energías convencionales. Existen numerosos diseños de secadores solares, en este ensayo se utilizó uno de tipo indirecto y con convección forzada. Se muestran los resultados obtenidos del secado en una experiencia realizada durante junio de 2015. Se utilizaron dos geometrías de muestras testigos, rodajas y cubos. En los resultados obtenidos se puede apreciar que la humedad en base húmeda para las rodajas disminuyó del 92 % al 9% y para muestras cortadas en cubos de 87 % a 37,7%. Los rendimientos instantáneos del colector integrado al secador varían entre 32 % y 74 %.","palabras_clave":"Secado solar|rendimiento colector|curva de secado|Cucurbita moschata"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6323,"idioma_id":4,"titulo":"Impact of pectin or chitosan on bulk, interfacial and antioxidant properties of (+)-catechin and b-lactoglobulin ternary mixtures","resumen":"The interactions effect between (+)-catechin, b-lactoglobulin and two types of polysaccharides (pectin and chitosan) were studied at pH 4.Dynamic light scattering (DLS) revealed that pectin formed smaller complexes in the presence of (+)-catechin and b-lg, while chitosan presented a reduced number of populations with higher particle size. Dynamic interfacial data (obtained by a drop tensiometer) revealed that the complexes formed between pectin, (+)-catechin and b-lg slowed down the b-lg migration to the oil\/water interface; contrarily to the chitosan mixtures where protein migrated faster. The surface dilatational modulus of b-lg was &#8764;45 mN\/m and increased to &#8764;60 mN\/m when mixing with pectin and (+)-catechin, while the values of the surface dilatational modulus for the chitosan mixed interfacial films decreased to &#8764;35 mN\/m. The free (+)-catechin content decreased 12% and 10% when interacting with pectin and chitosan, respectively, as compared to pure (+)-catechin. However, the (+)-catechin antioxidant activity were not affected by the interactions. Complexes formed between polyphenols, proteins and polysaccharides could be used as a good alternative to understand and consequently improve the phytochemicals stability in food matrices.","palabras_clave":"antioxidantes|interacciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6324,"idioma_id":4,"titulo":"Pathogenic ability and saline stress tolerance of two Fusarium isolates from Odontesthes bonariensis eggs","resumen":"Background: Several fungal species represent a potential risk to embryos of Odontesthes bonariensis(Cuvier and Valenciennes, 1835), a euryhaline freshwater fish that lives in the Pampean inland watersand has potential economic relevance.Aims: To identify two fungi isolated from O. bonariensis eggs exposed to saline conditions and to characterizetheir pathogenicity and tolerance to sodium chloride solutions.Methods: The isolates were identified by morphological features, and a preliminar phylogenetic analysisusing sequences of translation elongation factor 1-alpha (EF-1) and calmodulin (CAM) was performed.Koch?s postulates were tested to identify the causative agent of fungal infection. The influence of NaCl onthe fungal growth was evaluated in in vitro assays.Results: The isolates LPSC 1001 and 1002 were identified as representatives of the genus Fusarium, andbelonging to the Fusarium incarnatum-Fusarium equiseti species complex (FIESC) and the Fusarium solanispecies complex (FSSC), respectively. Histological observations on eggs exposed in vitro to both isolatesin infectivity assays confirmed the ability of the fungal isolates to penetrate to egg?s chorionic membrane,leading to the death of embryos. Increasing NaCl concentration in the culture medium reduced the growthof the isolates LPSC 1001 and 1002, being completely inhibited at 160 and 120 g\/l NaCl respectively.Conclusions: The isolates LPSC 1001 (FIESC) and 1002 (FSSC) were identified as fungal pathogens toO. bonariensis eggs. The use of NaCl solutions as antifungal treatment was not effective to control theinfection with these strains.","palabras_clave":"FISH PATHOGENS|FIESC - FSSC|FUSARIUM PATHOGENICITY|SALINE STRESS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6325,"idioma_id":4,"titulo":"Streamflow variability in the Chilean Temperate-Mediterranean climate transition (35°S?42°S) during the last 400 years inferred from tree-ring records","resumen":"As rainfall in South-Central Chile has decreased in recent decades, local communities and industries have developed an understandable concern about theirthreatened water supply. Reconstructing streamflows from tree-ring data has been recognized as a useful paleoclimatic tool in providing long-term perspectives on the temporal characteristics of hydroclimate systems. Multi-century longstreamflow reconstructions can be compared to relatively short instrumental observations in order to analyze the frequency of low and high water availability through time. In this work, we have developed a Biobío River streamflow reconstruction to explore the long-term hydroclimate variabilityat the confluence of the Mediterranean-subtropical and the Temperate-humid climate zones, two regions represented by previous reconstructions of the Maule and Puelo Rivers, respectively. In a suite of analyses, the Biobío River reconstruction proves to be more similar to the Puelo River than the Maule River, despite its closer geographic proximity to the latter. This finding corroborates other studies with instrumental data that identify 37.5°S as a latitudinal confluence of two climate zones. The analyzed rivers are affected by climate forcings on interannual and interdecadal time-scales, Tropical (El Niño Southern Oscillation) and Antarctic (Southern Annular Mode; SAM). Longer cycles found, around 80-years, are well correlated only with SAM variation, which explains most of the variance in the Biobío and Puelo rivers. This cycle also has been attributed to orbital forcing by other authors. All three rivers showed an increase in the frequency of extreme high and low flow events in the twentieth century. The most extreme dry and wet years in the instrumental record (1943?2000) were not the most extreme of the past 400-years reconstructed for the three rivers (1600?2000), yet both instrumental record years did rank in the five most extreme of the streamflow reconstructions as a whole. These findings suggest a high level of natural variability in the hydro-climatic conditionsof the region, where extremes characterized the twentieth century. This information is particularly useful when evaluating and improving a wide variety of water management models that apply to water resources that are sensitive to agricultural and hydropower industries.","palabras_clave":"Biobío River|Streamflow reconstructions|Southern Annular Mode|Hydroclimate variability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6326,"idioma_id":4,"titulo":"A review of genus Nysius Dallas in Argentina (Hemiptera: Heteroptera: Orsillidae)","resumen":"The orsillid genus Nysius Dallas is a complex and large genus with more than 100 described species worldwide, and includesmany species of agricultural importance, one such example is N. simulans (Stål) from Argentina. Three species ofNysius are found in this country: N. simulans (Stål), N. irroratus (Spinola) and N. puberulus Berg. The material availablefor the present study, consisting of over 610 specimens, was collected by sweep-net, G-Vac and light trap in the provincesof Chubut (42° to 46°S; 63.5° to 72° W), La Pampa (35º to 39º south; 63º to 68º West), Neuquén (36° to 41° S; 68° to71°W) and Río Negro (37° to 42°S; 62° to 71°W), in the central region of Argentina, during the years 2010?2014. Althoughthe species N. simulans is frequently recorded as a pest of soybean crop, there exist no detailed description but onlya brief general diagnosis. In the present contribution, therefore, we provide a detailed redescription as well as an updateddistribution of N. simulans and N. irroratus, and a key for species of genus Nysius present in Argentina.","palabras_clave":"TRUE BUGS|SOYBEAN|SPECIES COMPLEX|ORSELLIDAE|NYSIUS|KEY TO SPECIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6328,"idioma_id":5,"titulo":"Análisis de los trabajos finales de investigación en la Escuela de Fonoaudiología de la Universidad Nacional de Córdoba, Argentina, 1993-2014","resumen":"Los estudiantes universitarios producen un importante caudal de trabajos de investigación (TFI) para la obtención del título de grado. Esta producción contribuye a visualizar aspectos de la realidad en la cual una disciplina y\/o profesión está inmersa. El objetivo de este trabajo fue describir la producción de TFI según el año de producción, el tipo de vinculación del director y el área de desempeño disciplinar de la temática abordada, en la carrera de la licenciatura en fonoaudiología de la Facultad de Ciencias Médicas de la Universidad Nacional de Córdoba (UNC), Argentina, desde el año 1993 hasta el año 2014. Se llevó a cabo un estudio descriptivo en el que se analizaron 461 TFI. Los resultados mostraron una mayor participación de directores externos (n=86) que de directores docentes de la Escuela de Fonoaudiología (n=69) en el primer oncenio, mientras que en el segundo esta distribución se invierte (88 externos versus 138 docentes de la EF). En relación a las áreas de desempeño disciplinar se observó, tanto en el primer como segundo oncenio, un predominio del área de audiología con 77 y 59 TFI, respectivamente. El nivel de asociación resultó estadísticamente significativo entre los oncenios en las áreas lenguaje y fonoestomatología. Los resultados encontrados en la Escuela de Fonoaudiología de la UNC difieren de otros estudios realizados en Argentina y América Latina, el contexto histórico y científico pudo incidir en los cambios observados en los TFI. Se reconoce la necesidad de futuras investigaciones orientadas a analizar el balance entre necesidades de conocimientos y la producción científica, así como identificar áreas disciplinares y temas que requieren prioridad en la promoción de la investigación","palabras_clave":"fonoaudiología|investigación|área de desempeño disciplinar"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6329,"idioma_id":5,"titulo":"USO DE LA RADIACIÓN UV-C EN EL PROCESO DE ELABORACIÓN DE HORTALIZAS DE IV GAMA","resumen":"Uno de los principales factores que limita la vida útil de las hortalizas frescas es el crecimiento de microorganismos durante el almacenamiento refrigerado. Es por ello que durante la etapa de procesamiento de hortalizas de IV gama , siempre se incluye una etapa de lavado y sanitización para reducir la contaminación microbiana inicial. El uso de radiación ultravioleta artificial (UV) a una longitud de onda de 190 a 280 nm (UV-C), tiene poder germicida y puede ser eficaz para la descontaminación de la superficie de frutas y hortalizas. Sin embargo, su eficacia antimicrobiana puede estar influenciada por las características y composición del producto. El tratamiento con UV-C ofrece varias ventajas a los procesadores de alimentos, ya que no deja residuos, no tiene restricciones legales, es fácil de usar y letal para la mayoría de los tipos de microorganismos, y no requiere un equipo de seguridad complejo para ser implementado. De esta forma, en este trabajo se evalúa la aplicación de la radiación UV-C en el proceso de elaboración de hortalizas de IV gama y su efecto posterior sobre la vida útil. Palabras clave: UV-C, sanitización, hortalizas mínimamente procesadas, poscosecha.","palabras_clave":"UV-C|SANITIZACIÓN|HORTALIZAS|PROCESAMIENTO MINIMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6330,"idioma_id":4,"titulo":"Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determination","resumen":"In the present work, we report a microfluidic immunosensor with a nanostructured platform based in zinc oxide nanoparticles covered by polyvinyl alcohol (ZnONPs-PVA) coupled to laser-induced fluorescence (LIF) for detection of epithelial cell adhesion molecule (EpCAM). EpCAM is a cell surface protein, and is overexpressed by epithelial carcinomas as such as lung, colorectal, breast, prostate, head, neck, and hepatic origin. The detection limits (LODs) calculated for our method and for a commercial enzyme-linked immunosorbent assay (ELISA) test kit were 1.2 and 13.9 pg mL-1, respectively. The within- and between-assay variation coefficients for the proposed method were below 6.50%. The results correlated well with those obtained with the commercial ELISA method, thus demonstrating that the new nano-platform integrated into a microfluidic device offers a truthful and useful analytical tool to be easily applied in epithelial cancer diagnosis through of EpCAM biomarker determination.","palabras_clave":"Nanostructured platform|Microfluidic immunosensor|Cancer diagnostics|EpCAM biomarker|Epithelial cancer|Circulating tumor cells"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6331,"idioma_id":5,"titulo":"Problematización de los sucesos de derechos humanos en Argentina","resumen":"ExtractoLa cuestión es problematizar sobre la constitución del sujeto argentino, es decir, la experiencia de sí, en la Argentina de estos últimos años ; la de los tipos y estilos de gobiernos que establecemos los argentinos estando los derechos humanos implicados.Nos interesa en esta investigación es la constitución histórica de las diferentes formas del sujeto argentino, en este caso, en relación con los juegos de verdad que hemos construido para nosotros y para el mundo, cuando los derechos humanos son un factor central.Los modos que tienen de operar las organizaciones de derechos humanos han crecido y se han fortalecido debido a la existencia de ciertos sectores del poder que continúa negando y desmintiendo las desigualdades políticas y jurídicas de los que menos recursos económicos y sociales tienen ; pero, también se han desarrollado y producido saberes y conocimientos que se emparentan con las condenas penales de los actores de los delitos de lesa humanidad y con la condena a algunos civiles cómplices de la violencia económica y represiva sistemática.","palabras_clave":"Poder|Subjetividad|Derechos Humanos|Saber"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6332,"idioma_id":4,"titulo":"Responsive nanogels for application as smart carriers in endocytic pH-triggered drug delivery systems","resumen":"Various specially designed poly(N-isopropylacrylamide-co-acrylic acid) (NIPA-co-AAc) nanogels (NGs) with different NIPA\/AAc molar composition were synthesized by precipitation\/dispersion polymerization and evaluated as carriers for drug delivery systems (DDSs) of doxorubicin hydrochloride (DOXOHCl) for cancer therapies. The NGs presented excellent dispersability in physiological environments (pH 7.4 and 5 at 37 C), as shown by dynamic light scattering (DLS). Moreover, the NGs exhibited high drug loading capacity and efficiency due to the ionic interaction of the cationic drug with the anionic NGs. NGDOXO HCl formulation presented excellent dispersability in water and minimal leakageof the cargo at plasma simulated medium (pH 7.4 and 0.14 M NaCl) at 37 C and a triggered release at lysosomal simulated medium (pH 5 and 0.14 M NaCl). This release behavior together with their size and the low cytotoxicity determined by the MTT assay converts these NGs in great candidates for their application as carriers in cancer therapies based on the enhanced permeability and retention effect (EPR) with drug pH-triggered release after endocytosis in tumor cells.","palabras_clave":"nanogels"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6333,"idioma_id":4,"titulo":"Development and characterization of functional O\/W emulsions with chia seed (Salvia hispanica L.) by-products","resumen":"The present work investigated the physicochemical properties of O\/W emulsions containing functional ingredients (oil with high &#969;-3 fatty acid content, protein and\/or soluble fiber) from chia seeds. The effect of different protein-carbohydrate combinations (sodium caseinate and lactose, sodium caseinate and maltodextrin, chia protein-rich fraction and maltodextrin) and the presence of chia mucilage (0 and 0.2% wt\/wt) in the aqueous phase of chia O\/W emulsions was studied as a function of droplet size distribution, Sauter mean diameter, &#950;-potential, rheological properties and backscattering profiles. The use of sodium caseinate in combination with lactose or maltodextrin produced chia O\/W emulsions with small droplet size (0.22 to 0.27 µm), high degree of uniformity in droplet size distribution, negatively charged droplets (at pH 6.5), pseudoplastic behavior and high physical stability. Emulsions with chia protein-rich fraction presented wider droplet size distribution and higher D[3,2] values than the previous ones, recording a Newtonian behavior. The addition of chia mucilage affected the physicochemical properties studied, mainly the rheological characteristics of emulsions.","palabras_clave":"CHIA SEED OIL|MUCILAGE|CHIA PROTEIN-RICH FRACTION|O\/W EMULSIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6334,"idioma_id":4,"titulo":"A novel series of forepaw muscles for mammals observed in the Patagonian weasel Lyncodon patagonicus","resumen":"The intrinsic autopodial musculature of mammals is composed of a relatively stable number of muscular groups. Here, we present the first myological studies of Lyncodon patagonicus, a South American mustelid that is morphologically and ecologically poorly known. The typical intrinsic muscles of the manus of Lyncodon are similar to those of its sister taxon Galictis, although there exist few variations, including additional subdivisions and relatively more distal insertions in some muscles. A striking feature is the presence of a novel series of forepaw muscles never before described for mammals. These muscles, named here mm. flexores digitorum proprii manus, are represented by a medial and a lateral belly for each digit and are located immediately distal to the mm. flexores breves profundi. They are located distally alongside the tendons of the latter, along the palmar aspect of the phalangeal series, and they insert onto the flexor tubercle of the distal phalanx of each digit. We propose that the incorporation of these muscles in the forepaw musculature of Lyncodon, in addition to some other osteo-myological features, imply a distinctivemorpho-functional adaptation to increase the force of flexion and independence of digital movements likely used to grasp prey during hunting. This hypothesis should be confirmed by future ecological and behavioral studies on this species.","palabras_clave":"DEXTERITY|FORELIMB|INTRINSIC FOREPAW MUSCULATURE|LYNCODON"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6335,"idioma_id":4,"titulo":"Comparisons between singularities of pseudophase and speckle phase using binary diffusers in optical vortex metrology","resumen":"An analysis based on the comparison between singularities of speckle phase and pseudophase in the practice of optical vortex metrology is carried out by measuring the phase map of the speckle pattern obtained from the transmitted light through binary diffusers. In the characterization of the core structure of both phase singularities, the variation of the measured parameters is produced by means of in-plane linear displacements and rotations of the scattered speckle fields. These fields are addressed by using similar displacements of the binary phase masks recorded in a spatial light modulator (SLM). We complete these comparisons by measuring out-of-plane variations of the core structure parameters. In addition, we verified that the phase map of the transmittedlight beam through the binary diffusers recorded in SLMs is actually characterized by a speckle phase with vortices of unitary charge. The presented analysis would be helpful for understanding the scope and limitations of the use of the singularities of speckle phase and pseudophase as position marking, and also for speckle measurement of in-plane rigid-body displacements of binary diffusers dynamically controlled by means of SLMs.","palabras_clave":"speckle|optical vortex metrology|phase retrieval| singular optics|spatial light modulator|binary diffuser"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6336,"idioma_id":4,"titulo":"Combined effects of litter features, UV radiation, and soil water on litter decomposition in denuded areas of the arid Patagonian Monte","resumen":"Aims We evaluated the combined effects of litter type, UV radiation, and soil water on litter decomposition processes in denuded areas of the arid Patagonian Monte. Methods We conducted a manipulative experiment with litterbags with litter dominated by evergreen shrubs (ES), and litter dominated by perennial grasses and evergreen shrubs (PG) placed on undisturbed upper soil blocks from denuded areas. We subjected soil blocks with litterbags to near ambient and attenuated UV radiation levels, and low and high soil water levels. We evaluated litter mass loss and changes in N, soluble phenolic and lignin contents during 420 days. Results PG litter decomposed faster than ES litter, and UV radiation and soil water enhanced decomposition of both litter types. PG litter immobilized N while ES litter released N. During decomposition, soluble phenolic content decreased while lignin content did not vary in both litter types. Conclusions Our results highlighted that abiotic and biotic controls differed between mass loss and N release\/immobilization. We found additive effects of the studied factors on mass loss while litter chemistry controlled microbial N release or immobilization from decaying litter. Further studies should explore the effects of these factors on species or functional groups shifts in microorganism communities.","palabras_clave":"C\/N RATIO|LITTER CHEMISTRY|FOTODEGRADATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6337,"idioma_id":5,"titulo":"MICROSPOROGÉNESIS DE TRES ESPECIES DEL SUBGÉNERO PASSIFLORA (PASSIFLORA, PASSIFLORACEAE) DE PARAGUAY","resumen":"The aim of the present study was to characterize karyologically species of Passiflora L. from Félix Pérez Cardozo District, Guairá, Paraguay. The chromosome numbers in meiosis 2n = 9II for P. alata Curtis and P. caerulea L. are reported for the first time. Chromosome number for P. edulis Sims (2n = 9II) is also confirmed. Meiotic behaviour in all studied species was normal with regular chromosome segregation. The most frequent chromosome associations were bivalents at diakinesis and metaphase I. Few meiotic irregularities were observed, such as lagging chromosomes and asynchronous phases of meiosis II, however, pollen viability was high, between 78.83% and 98.6%. The obtained results confirms x = 9 as the basic chromosome number for subgenus Passiflora. The present study is a rele¬vant contribution to the knowledge on the cytogenetics of species of Passiflora subg Passiflora from Paraguay.","palabras_clave":"PASSIFLORA|MEIOSIS|VIABILIDAD DE POLEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6338,"idioma_id":4,"titulo":"A Chemoenzymatic Route To Prepare Acyclic Nucleoside Analogues","resumen":"Acyclic nucleosides are potential antiviral and antitumor agents. In this work, their preparation through a novel chemoenzymatic procedure involving the N-alkylation of a nucleobase and the subsequent aldol condensation catalyzed by a dihydroxyacetone phosphate-dependant aldolase (Rabbit Muscle Aldolase, RAMA) is described.","palabras_clave":"Nucleosides|Enzyme catalysis|lyases|aldol reactions"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6340,"idioma_id":4,"titulo":"A new species of Mastigostyla (Iridaceae) from Argentina","resumen":"We describe and illustrate Mastigostyla coronata, a new species of Iridaceae from the high Andes of Jujuy (Argentina), morphologically similar to Mastigostyla vargasii. The inclusion of this new species in Mastigostyla is supported by a phylogenetic analysis based on plastid markers. We also present a morphological comparison among the sub-acaulescent species of Mastigostyla (including Cardenanthus),and a key to those species.","palabras_clave":"ANDES|SOUTH AMERICA|TAXONOMY|TIGRIDIEAE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6341,"idioma_id":5,"titulo":"A Taxonomy-based Approach For Fault Localization In Service-Oriented Applications","resumen":"Service Oriented Computing is a relevant paradigm that allows for building software solutions as it supports the creation of dynamic and agile applications. As a result of these benefits, applications based on the paradigm are commonly used in the industry. However, the more complex software solutions become, the higher the chance of having bugs is. Identifying them in the entire solution may also be one of the most tedious, expensive and time-consuming activity within the developing process. Therefore, fault localization aims at finding the location of faults, determining the root cause of the failure and identifying the causes of abnormal behavior of a faulty program. In particular, fault localization for service oriented applications is currently a research field that has received little attention. In this context, we present a taxonomy-based approach for fault localization in service oriented applications, using information of faults in the underlying architecture of these applications, namely SOA. The results on the location of faults are not only provided to the developer but also enhanced with architectural information, that is, showing the faults and their connection with exceptions, code and architectural scenarios. The experimental results carried out indicate that developers reduce their effort of localizing faults in terms of time and lines of code.","palabras_clave":"SERVICE-ORIENTED|TAXONOMY|FAULT LOCALIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6342,"idioma_id":5,"titulo":"FERTILIZACIÓN NITROGENADA EN LA REGIÓN SUBHÚMEDA BONAERENSE: EFICIENCIA DEL USO DEL AGUA Y DEL NITRÓGENO","resumen":"El agua y el nitrógeno (N) son típicamente los principales factores de producción del trigo en regiones con limitantes hídricas. Por esto, es de gran importancia incrementar la eficiencia de uso del N (EUN) - cuando se aplican fertilizantes - y la eficiencia del uso del agua (EUA) en ambientes subhúmedos. Los objetivos de este trabajo fueron i) analizar la respuesta del trigo a diferentes dosis de N, ii) evaluar la EUNy EUA de la biomasa total aérea (MST) y rendimiento en grano, y iii) predecir estas eficiencias mediante modelos construidos a partir de componentes principales (CP) que expliquen la variabilidad en la producción de MST y rendimiento, durante cinco años en un ambiente subhúmedo. Durante 2008-2012, se realizaron ensayos de fertilización nitrogenada sobre trigo utilizando cuatro dosis (0-25-50-100 kgha-1) aplicadas a la siembra. En madurez fisiológica se midió la MST y el rendimiento de grano, y se calculó la EUN y EUA para ambos parámetros. El análisis de CP se realizó utilizando al N disponible y humedad del suelo, y las diferentes precipitaciones en función de la MST y rendimiento. Se originaron tres CP para cada parámetro, con una explicación total de la varianza de 90% y 96% para MST y rendimiento. Las predicciones de EUN para MST y rendimiento, incorporaron los 3 CP con elevados valores de predicción (R2=0,78-0,81). Sin embargo, los ajustes fueron bajos (R2= 0,38-0,45) parala EUA de MST y grano, excluyendo del modelo al CP influenciado por N disponible. Con alta variabilidad en la disponibilidad de agua la fertilización con N no permitió el incremento significativo dela EUA, demostrando que el factor agua es el más limitante para este ambiente. La utilización de CP como regresoras permitió generar modelos certeros de predicción dela EUN de MST y del rendimiento.","palabras_clave":"TRITICUM AESTIVUM L|PRECIPITACIONES ERRATICAS|COMPONENTES PRINCIPALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6343,"idioma_id":4,"titulo":"Maize and cover crop sequence in the Pampas: effect of fertilization and water stress on the fate of nitrogen","resumen":"Cover crops are well known for their positive effects on erosion processes, soil organicmatter, soil physical properties, weed populations and nitrate leaching. In this work, weevaluated the fate of nitrogen (N) from fertilizer in maize (Zea mays) and then inryegrass (Lolium multiflorum) as cover crop, in the conditions of the Argentine Pampas.To this end, a field experiment was carried out at the School of Agriculture, Universityof Buenos Aires, Argentina (34°36´ S, 58°29´ W). The design of the experiment was afactorial with three replications. We applied to maize 2 levels of N (0 and 140 kg N ha-1(125 lb N ac-1) (ammonium nitrate target with 15N) and two levels of water (50 and100% of crop evapotranspiration). 15N was determined in both the soil and plants.Maize plants and the soil organic fraction were the main sinks of fertilizer N, dependingon the water treatment. The N from fertilizer remaining as nitrates in the soil (0 to 1.50m [0 to 4.92 ft] depth) at maize harvest was 8% in plots subjected to water stresscompared to 3% in the non-water stressed. Nitrogen losses due to volatilization wereminor. Total N (soil and fertilizer) accumulated in ryegrass tissues plus nitratesremaining in the soil were higher in cover crop plots than in bare soil (130 vs. 51 kg Nha-1 [116 vs. 45.5 lb N ac-1]). The N in the soil organic matter originating from fertilizersignificantly decreased between maize harvest and cover crop harvest. This soilorganic N that originated from fertilizer mineralized at high rate (around 47% in 6months), suggesting it was in more labile. This mineralized N can be subjected topotential losses during following months","palabras_clave":"CULTIVOS DE COBERTURA|MAIZ|NITRATOS|CONTAMINACION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6344,"idioma_id":5,"titulo":"Recuperación y destino del fertilizante foliar en trigo usando la técnica isotópica del 15N.","resumen":"Se realizó un experimento en el Sud Oeste Bonaerense con dos dosis de fertilización inicial (0 y 50 kg N ha-1) y dos dosis de fertilización foliar (0 y 25 kg N ha-1). La aplicación foliar con urea enriquecida, se realizó en antesis y post antesis, en microparcelas. Los rendimientos fueron bajos sin respuesta a la fertilización inicial ni a la foliar: 2100 y 2154 kg ha-1 para 0 y 50N sin N foliar y 2160 y 2303 kg ha-1 con N foliar. En la parcela isotópica, enriquecida con 15N, el trigo acumuló 83 kg N ha-1 en la biomasa aérea y 52 kg N ha-1 en el grano sin efecto significativo con la fertilización inicial. El N proveniente del fertilizante foliar marcado se acumuló: 20% en la materia seca aérea (MSA) y 12 % en el grano, con una recuperación de 6 kg N por kg de grano ha-1. El N acumulado en la paja rondó los 10 kg N ha-1 y el no recuperado por ningún componente del trigo, varió entre 7,2 y 8,3 kg N ha-1, rondando el 30%. Con el método de la diferencia, la MSA obtuvo una ERN entre 30 a 54% para los tratamientos 0N y 50N iniciales respectivamente. El grano fue menos eficiente (30 a 34% de recuperación) y acumuló 2,4 a 6 kg N por kg grano ha-1. El isotópico detectó una ERN de 70% en MSA y 25% en el grano. El grano acumuló nuevamente 6 kg N ha-1. Las diferencias entre los métodos, sugieren identificar exhaustivamente los factores que influyen en estas estimaciones.","palabras_clave":"Fertilización foliar |Trigo|Isótopos|15N"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6345,"idioma_id":4,"titulo":"Melanopsin and the Non-visual Photochemistry in the Inner Retina of Vertebrates","resumen":"Melanopsin (Opn4), a member of the G protein-coupled receptor family, is a vitamin A-based opsin in the vertebrate retina that has been shown to be involved in the synchronization of circadian rhythms, pupillary light reflexes, melatonin suppression and other light-regulated tasks. In non-mammalian vertebrates there are two Opn4 genes, Opn4m and Opn4x, the mammalian and Xenopus orthologs, respectively. Opn4x is only expressed in non-mammalian vertebrates including reptiles, fish and birds, while Opn4m is found in a subset of retinal ganglion cells (RGCs), the intrinsically photosensitive (ip) RGCs of the inner retina of both mammals and non-mammalian vertebrates. All opsins described utilize retinaldehyde as chromophore, photoisomerized from 11-cis- to all-trans-retinal upon light exposure. Visual retinal photoreceptor cones and rods, responsible for day and night vision respectively, recycle retinoids through a process called the visual cycle that involves the retinal pigment epithelium (RPE) or glial Müller cells. Although Opn4 has been characterized as a bistable photopigment, little is known about the mechanism\/s involved in its chromophore regeneration. In this review, we will attempt to shed light on the visual cycle taking place in the inner retina and discuss the state of the art in the non-visual photochemistry of vertebrates.","palabras_clave":"ciclo visual|retina interna|melanopsina|retinal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6346,"idioma_id":4,"titulo":"The Visual Cycle in the Inner Retina of Chicken and the involvement of Retinal G-Protein-coupled Receptor (RGR)","resumen":"The vertebrate retina contains typical photoreceptor (PR) cones and rods responsible for day\/night vision respectively, and intrinsically photosensitive retinal ganglion cells (ipRGCs) involved in the regulation of non-image forming tasks. Rhodopsin\/cone opsin photopigments in visual PRs or melanopsin [Opn4] in ipRGCs utilize retinaldehyde as chromophore. The retinoid regeneration process denominated ?visual cycle? involves the retinal pigment epithelium (RPE) or Müller glial cells. Opn4, on the contrary, has been characterized as a bi\/tristable photopigment, in which a photon of one wavelength isomerizes 11-cis to all-trans retinal (Ral), with a second photon re-isomerizing it back. However, it is unknown how the chromophore is further metabolized in the inner retina. Nor is it yet clear whether an alternative secondary cycle occurs involving players such as the retinal G-protein-coupled receptor (RGR), a putative photoisomerase of unidentified inner retinal activity. Here we investigated the role of RGR in retinoid photoisomerization in Opn4x (Xenopus ortholog) (+) RGC primary cultures free of RPE and other cells from chicken embryonic retinas. Opn4x (+) RGCs display significant photic responses by calcium fluorescent imaging and photoisomerize exogenous all-trans to 11-cis Ral and other retinoids. RGR was found to be expressed in developing retina and in primary cultures; when its expression was knocked down, levels of 11-cis, all-trans Ral and all-trans retinol in cultures exposed to light were significantly higher and those in all-trans retinyl esters lower than in dark controls. Results support a novel role for RGR in ipRGCs to modulate retinaldehyde levels in light keeping the balance of inner retinal retinoid pools.","palabras_clave":"visual|inner retina|retinoids|melanopsin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6347,"idioma_id":4,"titulo":"Introducing the concept of biocatalysis in the classroom: the conversion of cholesterol to provitamin D3","resumen":"Biocatalysis is a fundamental concept in biotechnology. The topic integrates knowledge of several disciplines; therefore, it was included in the course ?Design and optimization of biological systems? which is offered in the Biochemistry curricula. We selected the ciliate Tetrahymena as an example of a eukaryotic system with potential for the biotransformation of sterol metabolites of industrial interest; in particular, we focused on the conversion of cholesterol to provitamin D3.The students work with wild type and recombinant strains and learn how sterol pathways could be modified to obtain diverse sterol moieties. During the course the students identify and measure the concentration of sterols. They also search for related genes by bioinformatic analysis. Additionally, the students compare biotransformation rates, growing the ciliate in plate and in a bioreactor. Finally, they use fluorescence microscopy to localize an enzyme involved in biotransformation. The last day each team makes an oral presentation, explaining the results obtained and responds to a series of key questions posed by the teachers, which determine the final mark. .&nbsp; In our experience, this course enables undergraduate students to become acquainted with the principles of biocatalysis as well as with standard and modern techniques, through a simple and robust laboratory exercise, using a biological system for the conversion of valuable pharmaceutical moieties.&nbsp;","palabras_clave":"classroom|Tetrahymena"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6348,"idioma_id":4,"titulo":"Mitigation of pulse width modulation distortion using a digital predistorter based on memory polynomials","resumen":"This paper presents an adaptive digital predistorter (DPD) that significantly reduces thebaseband distortion of digital pulse width modulation (PWM), typically used in switching(class-D) amplifiers. A generalized Hammerstein structure (also known as power filter)composed by static nonlinearities and FIR filters is used to model the baseband behaviorof PWM.We show that the contribution of th ehigher order terms of the nonlinearity arenegligible and therefore, for practical applications, only the first three or four odd powersshould be retained.The convergence of the DPD is studied and the performance isdemonstrated and compared with other approaches. Good results are obtained for typicalsignals even when using low-order FIR filters in the DPD. Measurement results obtainedusing a digital signal processor are also presented aiming to validate the proposedapproach.","palabras_clave":"PULSE WIDTH MODULATION|BASEBAND|DIGITAL PREDISTORTER|INDIRECT LEARNING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6349,"idioma_id":4,"titulo":"Assessing the effects of demersal fishing and conservation strategies of marine mammals over a Patagonian food web","resumen":"The San Matías Gulf (SMG) is a semi-enclosed ecosystem where the Argentine hake Merluccius hubbsi (AH) has been the main fishery resource since 1971. During the 20th century the South American sea lion Otaria flavescens (SASL) population was severely reduced in this ecosystem due an intense hunting, but in the 1970s conservation was promoted and hunting was banned. As a consequence SASL have been slowly recovering, until the 1990s when rapidly  rebuilt their populations. Recent studies indicate that they feed mostly over commercially profitable medium-sized AH. Also, medium-sized and large hake are well-known cannibals that feed heavily over smaller AH. Fishing trawlers affect juvenile and medium-sized AH and artisanal long-liners capture exclusively large AH. The objective of this study was to evaluate the effect of a growing SASL population over the AH in the SMG, considering the changes in the fishing activity as well as the size-structured cannibalism within the AH population. The evaluations were based on time series of hake and SASL biomass using the Ecopath with Ecosim dynamic modelling approach. The analyses show that over 4 decades the increase in Sea Lions biomass has not generated a significant increase in the predation mortality over AH. On the other hand, an increase and subsequent variations in the fishing mortality seems to be related with long-term variations in large AH abundance, and a decrease in medium-sized AH abundance, constraining the cannibalism mortality. This led to a positive effect over juvenile AH abundance due to a released cannibalism pressure, even considering  that the less abundant larger AH are also the main spawners of the population. In this scenario, where it seems that trawlers and long-liners have replaced several predators over the SMG food web, SLAS population is still growing but they may not reach the pristine abundance levels of the time before hunting.","palabras_clave":"Demersal fishing|Food web|South American sea lion|Argentine hake|Ecopath with Ecosim|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6350,"idioma_id":4,"titulo":"USE OF UV-C AND GASEOUS OZONE AS SANITIZING AGENTS FOR KEEPING THE QUALITY OF FRESH-CUT ROCKET (ERUCA SATIVA MILL.).","resumen":"The effects of UV-C radiation (5, 10 and 20 kJ m-2) and gaseous ozone (1, 2 and 5 ppm) on15 microbial growth and quality parameters of fresh-cut rocket (Eruca vesicaria subsp. Sativa)16 leaves throughout 12 days at 5 °C were investigated. As control, washing with water was17 used. Sensory attributes (general appearance, decay, color and odor), headspace gas18 composition, some chemical characteristics (chlorophyll a and b, total chlorophyll and total19 carotenoids) and microbial counts throughout shelf-life were studied.20 Fresh-cut rocket was not adversely affected by the UV-C and ozone treatment, maintaining21 the sensory quality during cold storage. The 20 kJ UV-C m-2 treatment was found to be22 better than the chlorine and gaseous ozone treatments, in terms of reducing the microbial23 load in fresh-cut rocket during 8 days of storage at 5 ºC","palabras_clave":"MINIMAL PROCESSING|UV-C|OZONE|ROCKET"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6351,"idioma_id":4,"titulo":"Ghrelin Binding to Serum Albumin and its Biological Impact","resumen":"Ghrelin is an octanoylated peptide hormone that plays a key role in the regulation of the body weight and glucose homeostasis. In plasma, ghrelin circulates bound to larger proteins whose identities are partially established. Here, we used size exclusion chromatography, mass spectrometry and isothermal titration microcalorimetry to show that ghrelin interacts with serum albumin. Furthermore, we found that such interaction displays an estimated dissociation constant (KD) in the micromolar range and involves albumin fatty-acid binding sites as well as the octanoyl moiety of ghrelin. Notably, albumin&#8210;ghrelin interaction reduces the spontaneous deacylation of the hormone. Both in vitro experiments?assessing ghrelin ability to inhibit calcium channels?and in vivo studies?evaluating ghrelin orexigenic effects?indicate that the binding to albumin affects the bioactivity of the hormone. In conclusion, our results suggest that ghrelin binds to serum albumin and that this interaction impacts on the biological activity of the hormone.&nbsp; ","palabras_clave":"Ghrelin|albumina|obesidad|interaccion de proteinas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6352,"idioma_id":4,"titulo":"Biochemical profiling study in umbilical cord blood as predictors of neonatal damage in different maternal pathologies","resumen":"Objective: To analyze different biochemical parameters (BP) in umbilical cord blood (UCB) of newborn from healthy mothers and with different maternal pathologies.Design: Prospective, consecutive and transversal studySetting: Biochemical profiling study of pregnant women and newborns in Argentina.Participants: Samples from UCB (835) were analyzed of newborn from mothers who attended at Obstetrics Division from January 2010 to May 2015. Delivery: cesarean. Newborn were classified controls (C) (healthy newborns from healthy mothers) (n=301) and pathological (P) (pathological newborns from pathological mothers) (n=534). Pathological mothers were classified into 6 groups: genital infections (226), metabolic pathologies (121), hypertension-preclampsia (73), immunological (63), cholestasis (26) and neoplasic (25) pathologies. Pathological newborn: IGR and\/or fetal distress.Outcome measure: Glucose (Glu), urea, creatinine, uric acid (UA), total bilirubin (TBi), total proteins (Pt), albumin (Alb), transaminases (ALT\/AST),  alkaline-phosphatase, gammaglutamyltranspeptidase (GGT), creatinkinasa (CK), lactatedehydrogenase (LDH),  amylase, pseudocholinesterase, iron, calcium, phosphorus, magnesium, sodium, potassium (K), chlorine, cholesterol (Chol), HDL-Chol, LDL-Chol, triglycerides(TG), hsCRP were determined by recommended methods. Tuckey?s and ¨Kruskal-Wallis tests were applied, p<0.05.Results: -P newborn from P mothers showed significant decrease: in gestation weeks (GW) and newborn weight (NW) in all the maternal pathologies with respect to C newborn from C mothers (p<0.01). -P mothers with metabolic, hypertension and neoplasic pathologies showed significant increases in age with respect to C mothers (p: < 0.01). -P newborn from mothers with different maternal pathologies showed significant increases and\/or decreases in several biochemical parameters in UCB with respect to C newborn.Conclusions: In P newborn the decrease in GW and NW would be related to IGR that accompany these pathologies. The increases observed of the analyzed parameters would be related both to cellular destruction associated to maternal pathologies in some parameters of some pathologies, as the maternofetal transfer of others across the placenta; and decreases of the parameters to IGR with hepatic immaturity.","palabras_clave":"Biochemical profiling study|umbilical cord blood |neonatal damage |maternal pathologies"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6353,"idioma_id":4,"titulo":"Biochemical parameters in pleural fluids: New contributions in the etiologic diagnosis of body fluids","resumen":"Objectives: The aim of this study was to evaluate the usefulness of different cutoffs applied to the cellularity and various biochemical parameters (BP) (metabolic and enzymatic) to contribute to the etiologic diagnosis of pleural fluids (PF). Design and Methods: We studied 150 samples from patients with pleural effusion, admitted to the Clinical Hospital. The cell count was total\/mm3 (TCC) and differential. The simultaneous determination in pleural fluid (PF) and serum (S) of BP were performed on Roche Hitachi 917 autoanalyzer: Glucose (GLU), protein (PT), albumin (ALB), cholesterol (COL), triglycerides (TG), lactate dehydrogenase (LDH), creatine kinase (CK), alanine aminotransferase (ALT), aspartateaminotransferase (AST), alkaline phosphatase (FAL), amylase (AMI), total bilirubin (BT). Statistical","palabras_clave":"Pleural Fluids|Etiologic Diagnosis|Biochemical Parameters"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6354,"idioma_id":5,"titulo":"Estado actual de la gonorrea","resumen":"La gonorrea es una infección de transmisión sexual conocida desde la antigüedad que se ha perpetuado en el tiempo. Su agente etiológico, Neisseria gonorrhoeae, fue adquiriendo resistencia a la mayoría de los antimicrobianos utilizados para su tratamiento empírico.Las resistencias emergentes en los últimos años son a fluorquinolonas, macrólidos y cefalosporinas orales y parenterales. Hay aislamientos multiresistentes que plantean un desafío para su tratamiento. En países donde estos hallazgos ocurrieron precedentemente, la experiencia de tratamientos con antibióticos no convencionales por Ej. aminoglucósidos es limitada  y también emergieron cepas resistentes. Una de las estrategias utilizadas frente a la sensibilidad disminuida a ceftriaxona es aumentar la dosis o utilizar tratamientos combinados.En el marco actual de la multiresistencia de este microorganismo es importante promover la prevención de la infección, realizar vigilancia epidemiológica  y buscar nuevas estrategias para su tratamiento.","palabras_clave":"GONORREA|NEISSERIA GONORRHOEAE|ANTIBIOTICOS|RESISTENCIAS EMERGENTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6355,"idioma_id":5,"titulo":"Investigación de microorganismos en sangre de cordón umbilical y en el             tracto genital materno como marcadores predictivos de daño neonatal.","resumen":"IntroducciónDada la complejidad existente en las múltiples etiologías que conducen al daño neonatal, el diagnóstico precoz no pueda probablemente ser asociado a un único indicador bioquímico, sino deberá considerarse como un desequilibrio conjunto entre la respuesta inflamatoria y el desbalance del contenido vaginal. Objetivos Identificar microorganismos del tracto genital materno que puedan resultar de utilidad como marcadores precoces de sufrimiento fetal, a fin de prevenir complicaciones perinatológicas en futuras situaciones de embarazo.  Pacientes y Métodos Se estudiaron en forma consecutiva y prospectiva 711 pacientes embarazadas que concurrieron al consultorio externo de Obstetricia del Hospital de Clínicas de la UBA, durante el periodo de enero 2010 a julio 2013 para sus controles prenatales. El estudio de la sangre de cordón umbilical incluyó la detección de: U. urealyticum y M. hominis a través de la utilización de sustratos metabólicos mediante el método comercial Micofast-Biomerieux; de T. vaginalis, mediante PCR con primers específicos; y de T. cruzi, por el método del microtubo, por la técnica de gota gruesa y extendido fino coloreado con Giemsa.     Se efectuó el estudio microbiológico del contenido vaginal de 288 pacientes embarazadas en la semana 35-37 por metodología convencional, incorporándose además el cultivo en tioglicolato modificado para la detección de T. vaginalis. Se investigó también EGB, en hisopado ano-rectal y introito vaginal, enriquecido en caldo selectivo y sembrado en medio cromogénico. Se utilizó el test de &#967;² Yates y el test de Fisher para muestras independientes, considerándose significativo p &#8804; 0,05. Resultados Los patógenos que se relacionaron en forma estadísticamente significativos con daño neonatal fueron M.hominis (p = 0,03), T.vaginalis (p Fisher = 0,03) y VB (p = 0,02). Donde las  complicaciones que predominaron fueron el  parto pretérmino, RPM, bajo peso y bajo apgar. No se asociaron a daño neonatal U. urealyticum (p = 0,35), Candida spp  (p =0,94) y EGB (p=0,18).Conclusión Dado que diferentes microorganismos del tracto genital materno se relacionaron significativamente con daño neonatal, resulta de fundamental importancia realizar el estudio microbiológico del contenido vaginal durante el embarazo a fin de prevenir posibles complicaciones maternas y perinatológicas.","palabras_clave":"Microorganismos|sangre de cordon|tracto genital materno|daño neonatal"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6356,"idioma_id":4,"titulo":"Skipped spawning in the Patagonian stock of Argentine hake (Merluccius hubbsi)","resumen":"We analyzed macroscopic and histological maturity data of Argentine hake (Merluccius hubbsi) collected off Argentina in the spawning area of the Patagonian stock of this species between 2005 and 2014 to determine the frequency of skipped spawning. This study revealed that the only category of this phenomenon observed for Argentine hake was the resting stage. The analysis of 58,891 adult females of Argentine hake sampled during their reproductive peak indicates that, annually, between 6% and 22% of them were skipped spawners. These females were located mostly in the periphery of the spawning area, in deeper (>90 m) and colder waters (6-7°C). Individuals that had skipped spawning had a more intense feeding activity and a better nutritional condition than did females collected in the main spawning area, evaluated by Fulton´s condition index (K=0.68-0.75). In contrast, postspawning females showed the poorest condition (K=0.62-0.68) because of the energy cost involved in reproduction. Skipped spawners were mostly young females with a modal age of 3 years and a modal size of 38 cm TL. These results indicate that a significant proportion of individuals that already had their first annual spawning could skip the next one and stay on the periphery of the reproductive area to feed.","palabras_clave":"SKIPPED SPAWNING|NUTRITIONAL CONDITION|SIZE STRUCTURE|FEEDING ACTIVITY|MERLUCCIUS HUBBSI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6357,"idioma_id":4,"titulo":"IL-8, GRO and MCP-1 produced by hepatocellular carcinoma microenvironment determine the migratory capacity of human bone marrow-derived mesenchymal stromal cells without affecting tumor aggressiveness","resumen":"New therapies are needed for advanced hepatocellular carcinoma (HCC) and the use of mesenchymal stromal cells (MSCs) carrying therapeutic genes is a promising strategy. HCC produce cytokines recruiting MSCs to the tumor milieu and modifying its biological properties. Our aim was to study changes generated on human MSCs exposed to conditioned media (CM) derived from human HCC fresh samples and xenografts. All CM shared similar cytokines expression pattern including CXCL1-2-3\/GRO, CCL2\/MCP-1 and CXCL8\/IL-8 being the latter with the highest concentration. Neutralizing and knockdown experiments of CCL2\/MCP-1, CXCL8\/IL-8, CXCR1 and CXCR2 reduced in vitro MSC migration of &#8805;20%. Simultaneous CXCR1 and CXCR2 neutralization resulted in 50% of MSC migration inhibition. MSC stimulated with CM (sMSC) from HuH7 or HC-PT-5 showed a 2-fold increase of migration towards the CM compared with unstimulated MSC (usMSC). Gene expression profile of sMSC showed ~500 genes differentially expressed compared with usMSC, being 46 genes related with cell migration and invasion. sMSC increased fibroblasts and endothelial cells chemotaxis. Finally, sMSC with HuH7 CM and then inoculated in HCC tumor bearing-mice did not modify tumor growth. In this work we characterized factors produced by HCC responsible for the changes in MSC chemotactic capacity with would have an impact on therapeutic use of MSCs for human HCC","palabras_clave":"mesenchymal stem cells|hepatocellular carcinoma|IL-8|Gro|MCP-1|migration"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6358,"idioma_id":4,"titulo":"Polyelectrolytes-Protein complexes: a viable platform in the downstream processes of industrial enzymes at scaling up level-MP-","resumen":"This review analyzes the potential application of the complex formation between proteins and natural or synthetic polyelectrolytes as tool for the isolation and purification of enzymes at scaling up level.RESULTS:Complexation between proteins and polyelectrolytes through electrostatic interactions gives either soluble or non-soluble complexes, leading to phase separation.The formation and the stability of these complexes are influenced by pH, ionic strength, ratio between protein and polyelectrolyte and charge density of protein and polyelectrolyte.CONCLUSION:We presented the advantages on the protein precipitation by adding a polyelectrolyte solution to a natural enzyme source (microbiological, vegetal or animal homogenate), over the classical precipitation methods. An enzyme can be precipitated like non-soluble complex, separated and then re-dissolved, thus the method works, at the same time, like concentrative and pre-purification steps together.","palabras_clave":"POLYELECTROLYTES|ENZYME|PURIFICATION|BIOSEPARATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6359,"idioma_id":4,"titulo":"Diversity of endophytic fungal and bacterial communities in Ilex paraguariensis grown under field conditions","resumen":"The composition and diversity of the endophytic community associated with yerba mate (Ilex paraguariensis) was investigated using culture-depending methods. Fungi were identified based on their micromorphological characteristics and internal transcribed spacer rDNA sequence analysis; for bacteria 16S rDNA sequence analysis was used. Fungal and bacterial diversity did not show significant differences between organ age. The highest fungal diversity was registered during fall season and the lowest in winter. Bacterial diversity was higher in stems and increased from summer to winter, in contrast with leaves, which decreased. The most frequently isolated fungus was Fusarium, followed by Colletotrichum; they were both present in all the sampling seasons and organ types assayed. Actinobacteria represented 57.5 % of all bacterial isolates. The most dominant bacterial taxa were Curtobacterium and Microbacterium. Other bacteria frequently found were Methylobacterium, Sphingomonas, Herbiconiux and Bacillus. Nitrogen fixation and phosphate solubilization activity, ACC deaminase production and antagonism against plant fungal pathogens were assayed in endophytic bacterial strains. In the case of fungi, strains of Trichoderma, Penicillium and Aspergillus were assayed for antagonism against pathogenic Fusarium sp. All microbial isolates assayed showed at least one growth promoting activity. Strains of Bacillus, Pantoea, Curtobacterium, Methylobacterium, Brevundimonas and Paenibacillus had at least two growth-promoting activities, and Bacillus, Paenibacillus and the three endophytic fungi showed high antagonistic activity against Fusarium sp. In this work we have made a wide study of the culturable endophytic community within yerba mate plants and found that several microbial isolates could be considered as potential inoculants useful for improving yerba mate production.","palabras_clave":"Endophytes|Yerba mate|Fungal diversity|Bacterial diversity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6360,"idioma_id":4,"titulo":"Natural Genetic Variation and Candidate Genes for Morphological Traits in Drosophila melanogaster","resumen":"Bodysize is a complex character associated to several fitness related traits thatvary within and between species as a consequence of environmental and geneticfactors. Latitudinal and altitudinal clinesfor different morphological traits have been described in several species of Drosophila andprevious work identified genomic regions associated with such variation in D. melanogaster. However, the genetic factorsthat orchestrate morphological variation have been barely studied. Here, ourmain objective was to investigate genetic variation for different morphologicaltraits associated to the second chromosome in natural populations of D. melanogaster along latitudinal and altitudinalgradients in Argentina. Our results revealed weak clinal signals and a strong populationeffect on morphological variation. Moreover, most pairwise comparisons betweenpopulations were significant. However, most of the populations showedsignificant differences among them, suggesting that this factor includesinformation regarding geographical gradients and genetic aspects. Our studyalso revealed important within-population genetic variation, which must beassociated to the second chromosome, as the lines are otherwise geneticallyidentical. In order to examinethe contribution of different candidate genes to natural variation for thesetraits. To this end, we performed quantitative complementation tests using abattery of lines bearing &nbsp;mutated allelesat candidate genes located in the second chromosome and six second chromosomesubstitution lines derived from natural populations. The results of complementationtests &nbsp;revealed that natural variationat all candidate genes studied, invected, Fasciclin 3, toucan, Reticulon-like1, jing and CG14478, affects the studied characters suggesting that they areQuantitative Trait Genes for morphological traits. Finally, the differentphenotypic outcomes observed&nbsp; suggest that different naturalalleles of each gene might contribute to natural variation for morphologicaltraits. However, non-additive effects cannot be ruled out, as wild-derivedstrains differ at myriads of second chromosome loci that may interactepistatically with mutant alleles. ","palabras_clave":"body size|Drosophila|complementation test"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6361,"idioma_id":4,"titulo":"Solvent-free microwave-assisted synthesis of novel pyrazolo [4&#8242;, 3&#8242;: 5, 6] pyrido [2, 3-d] pyrimidines with potential antifungal activity","resumen":"Antimicrobial photodynamic therapy (aPDT) is an emerging alternative to treat infections based on the use of photosensitisers (PSs) and visible light. To investigate the fungicidal effect of PDT against azole-resistant Candida albicans strains using two PSs with a different mechanism of action, hypericin (HYP) and 1,9-dimethyl methylene blue (DMMB), comparing their efficacy and the reactive oxygen species (ROS) species involved in their cytotoxicity. Azole-resistant and the azole-susceptible C. albicans strains were used. Solutions of 0.5 and 4 McFarland inoculum of each Candida strain were treated with different concentrations of each PS, and exposed to two light-emitting diode light fluences (18 and 37 J cm _2). Mechanistic insight was gained using several ROS quenchers. The minimal fungicidal concentration of HYP for &#8805;3 log10 CFU reduction (0.5 McFarland) was 0.62 lmol l _1 for most strains, whereas for DMMB it ranged between 1.25 and 2.5 lmol l _1. Increasing the fluence to 37 J cm _2 allowed to reduce the DMMB concentration. Higher concentrations of both PSs were required to reach a 6 log10 reduction (4 McFarland). H2O2 was the main phototoxic species involved in the fungicidal effect of HYP-aPDT whereas 1O2 was more important for DMMB-based treatments. aPDT with either HYP or DMMB is effective in killing of C. albicans strains independent of their azole resistance pattern. HYP was more efficient at low fungal concentration and DMMB at higher concentrations.","palabras_clave":"PYRAZOLOPYRIDOPYRIMIDINES|O-AMINONITRILES |CYANOPYRIDINES|MICROWAVE IRRADIATION|ANTIFUNGAL ACTIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6362,"idioma_id":4,"titulo":"Abundance, Richness, Seasonal and Altitudinal Dynamics of Aquatic True Bugs (Heteroptera) in Mountain Wetlands of Argentina","resumen":"Mountain wetlands provide unique information because their biota ishighly specific and contributes significantly to regional diversity. The goalsof this study were assessing altitudinal and temporal variation in thedistribution of Heteropteran assemblages inmountain wetlands; and studying the phenology of the most abundant species. Allstages of Heteroptera and data on environmental variables were collectedmonthly over one year from five mountain wetlands. A simple community structure,and also a low richness of aquatic true bugs,composed of four Heteropteran families (Corixidae, Belostomatidae, Notonectidaeand Gelastocoridae), characterized our ponds. Richness and abundance variedtemporally and spatially (p&lt;0.0001); and increased at higher elevations. Additionally,the highest abundance of coldstenothermal species occurred at the highest altitudes where harsh conditions areprevalent. Ectemnostega (E.) quadrata and S. (T.) jensenhaarupi, both endemic to theAndes region, were the most abundant species. Both these species showedunivoltine cycles and overwintered as adults. Biodiversity of the Heteropterareached maximum values in summer when water temperature increased. Temporal and spatial monitoringstudies provide key information on the distribution, diversity, and habitatrequirements of Heteropteran species as well as on conservation and managementof these vulnerable habitats and species which are faced with climate warming.","palabras_clave":"AQUATIC HETEROPTERA COMMUNITY|PHENOLOGY|ARID ENVIROMENTS|HIGH MOUNTAIN PONDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6363,"idioma_id":5,"titulo":"?\tDiagnóstico de un caso de escabiosis en la ciudad de Buenos Aires","resumen":"La escabiosis humana es una infeccion de la piel producida por el acaro Sarcoptes scabiei variedad hominis. Produce una erupcion intensamente pruriginosa, de predominio nocturno, y se transmite por contacto directo o fomites, enespecial ropa, donde el parasito permanece viableentre 2 y 5 dias.","palabras_clave":"ESCABIOSIS |CIUDAD DE BUENOS AIRES|DIAGNOSTICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6364,"idioma_id":4,"titulo":"Selection of Maize Genotypes with Tolerance to Osmotic Stress Associated with Salinity","resumen":"AbstractThirteen different inbred lines in relation to the type of grain and life cycles were characterized by testing for osmotic stress associated with salinity. The identification of tolerant genotypes would be an effective strategy to overcome the saline stress. Osmotic stress reduces immediately the expansion of the roots and young leaves which determine a reduction in the size of the plant. A  completely randomized design was adopted to test seedlings under controlled conditions of light and temperature. Two treatments were used: 0 mM NaCl (as control) and 100 mM NaCl. After 15 days of complete salinization, the seedlings were harvested and several morphological traits were measured. The morphological traits of growth were leaf growth (Ar1, Ar2, Ar3 and Ar4), dry masses of shoot and root (SDM and RDM, respectively). Also, traits associated with water economy were registered: leaf water loss (LWL) and relative water content (RWC). The morphological traits were expressed in relative terms, while the traits associated with the economy of water were expressed in absolute terms. Uni and multivariate techniques were applied to identify genotypes with divergent behaviors to osmotic stress tolerance. Also, a Tolerance Index was employed to identify superior genotypes. Four clusters were obtained after applying a Cluster Analysis and Principal Component Analysis (PCA). The genotypes were compared to each other with a test of DMS. The results obtained with different statistical techniques converged. Some variables presented a differentialweight classification of genotypes. The morphological traits like RDM, SDM, Ar3, Ar4 and Ar5 were the most discriminating. Tolerance Index allowed to classify genotypes, thus SC2 and AD3 lines were that reached highest value of the index and therefore would be tolerant lines, while AF3 and LP3 had a low index and were seen as sensible.","palabras_clave":"Maize,  |Salinity,|Osmotic Stress Tolerance,| Selection Index"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6365,"idioma_id":4,"titulo":"Optimization of Trichomonas vaginalis Diagnosis during Pregnancy at a University Hospital, Argentina","resumen":"Abstract: The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) staining, and wet mount with sodium-acetate-formalin (SAF)\/methylene blue method. PCR for 18S rRNA gene as well as culture in liquid medium were performed. The sensitivity and specificity of the microscopic examinations were evaluated considering the culture media positivity or the PCR techniques as gold standard. The frequency of T. vaginalis infection was 6.2% by culture and\/or PCR, 5.2% by PCR, 4.7% by culture, 3.1% by SAF\/methylene blue method and 2.8% by direct wet smear and prolonged MGG staining. The sensitivities were 83.3%, 75.0%, 50.0%, and 45.8% for PCR, culture, SAF\/methylene blue method, and direct wet smear-prolonged MGG staining, respectively. The specificity was 100% for all the assessed methods. Microscopic examinations showed low sensitivity, mainly in asymptomatic pregnant patients. It is necessary to improve the detection of T. vaginalis using combined methods providing higher sensitivity, such as culture and PCR, mainly in asymptomatic pregnant patients, in order to prevent maternal and perinatal complications.","palabras_clave":"OPTIMIZATION |TRICHOMONAS VAGINALIS|UNIVERSITY HOSPITAL|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6366,"idioma_id":4,"titulo":"From Molecular Biology to Clinical Trials: Toward Personalized Colorectal Cancer Therapy","resumen":"During the past years, molecular studies through high-throughput technologies have led to the confirmation of critical alterations in colorectal cancer (CRC) and the discovery of some new ones, including mutations, DNA methylations, and structural chromosomal changes. These genomic alterations might act in concert to dysregulate specific signaling pathways that normally exert their functions on critical cell phenotypes, including the regulation of cellular metabolism, proliferation, differentiation, and survival. Targeted therapy against key components of altered signaling pathways has allowed an improvement in CRC treatment. However, a significant percentage of patients with CRC and metastatic CRC will not benefit from these targeted therapies and will be restricted to systemic chemotherapy. Mechanisms of resistance have been associated with specific gene alterations. To fully understand the nature and significance of the genetic and epigenetic defects in CRC that might favor a tumor evading a given therapy, much work remains. Therefore, a dynamic link between basic molecular research and preclinical studies, which ultimately constitute the prelude to standardized therapies, is very important to provide better and more effective treatments against CRC. We present an updated revision of the main molecular features of CRC and their associated therapies currently under study in clinical trials. Moreover, we performed an unsupervised classification of CRC clinical trials with the aim of obtaining an overview of the future perspectives of preclinical studies.","palabras_clave":"colon cancer|clinical trials|análisis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6367,"idioma_id":4,"titulo":"Preparation and characterization of polybutylene-succinate\/ poly(ethylene-glycol)\/cellulose nanocrystals ternary composites","resumen":"Ternary composites were prepared by twin screw extrusion from polybutylene-succinate (PBS), poly(ethylene-glycol) (PEG) and cellulose nanocrystals (CNC). The aim of the work is to improve the physical-mechanical properties of PBS by the addition of CNC. A PEG\/CNC masterbatch was prepared in order to achieve a good dispersion of hydrophilic CNC in the hydrophobic PBS. The influence of the nanoparticle content on the polymer properties was studied.Regarding the thermal properties fractioned crystallization phenomena of PEG was observed during cooling from the melt. No significant nucleating effect of the nanocellulose was observed.  The material containing 4wt% of CNC showed the best mechanical performance among the nanocomposites studied due to the combination of high modulus and elongation at break with a low detrimental in strength compared with the PBS\/PEG blend. Moreover, no nanocellulose agglomerations were observed in its FESEM micrograph.","palabras_clave":"BIODEGRADABLE|NANOPARTICLES|NANOWIRES AND NANOCRYSTALS|PROPERTIES AND CHARACTERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6368,"idioma_id":4,"titulo":"Avalanches in tip-driven interfaces in random media","resumen":"We analyse by numerical simulations and scaling arguments the avalanche statistics of 1-dimensional elastic interfaces in random media driven at a single point. Both global and local avalanche sizes are power-law distributed, with universal exponents given by the depinning roughness exponent &#950; and the interface dimension d, and distinct from their values in the uniformly driven case. A crossover appears between uniformly driven behaviour for small avalanches, and point-driven behaviour for large avalanches. The scale of the crossover is controlled by the ratio between the stiffness of the pulling spring and the elasticity of the interface; it is visible both in the global and local avalanche-size distributions, as in the average spatial avalanche shape. Our results are relevant to model experiments involving locally driven elastic manifolds at low temperatures, such as magnetic domain walls or vortex lines in superconductors.","palabras_clave":"AVALANCHES|PINNING|ELASTICITY|INTERFACES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6369,"idioma_id":5,"titulo":"TODOS LOS FUEGOS EL FUEGO. Discusión en torno a las categorías modernas en la interpretación de registros antracológicos en contextos prehispánicos y coloniales","resumen":"En el presente texto discutimos los resultados de los análisis antracológicos de dos diferentes tipos de contexto recuperados en Argentina. Las ocupaciones coloniales e hispano indígenas de la ciudad de Mendoza iniciadas a mediados del siglo XVI, y del valle de Ambato, en el noroeste argentino, ocupado a lo largo del 1er milenio por grupos que la arqueología definió como Cultura Aguada. Ensayamos aquí un análisis que desplace la mirada de las plantas como meros recursos dialogando con algunas fuentes  etnográficas e históricas. Nos centramos particularmente en resultados ?no esperables? en términos de lógicas que enfatizan sobre cuestiones economicistas o funcionales.","palabras_clave":"ANTRACOLOGÍA|CATEGORÍAS|MODERNIDAD|COLONIAL|PREHISPÁNICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6370,"idioma_id":4,"titulo":"Plant functional types and trait values in the Paraná River floodplain: modelling their association with environmental features","resumen":"Our aim was to assess whether plant trait values and functional types are associated with environmental features in floodplain wetlands. The study was conducted in the Lower Paraná River floodplain, mostly dominated by marsh communities. In 72 sites, 16 functional traits were screened for 95 plant populations of 44 species. Since the interspecific variability in trait values resulted higher than the intraspecific variability, plant species (and not populations) were hierarchically classified into plant functional types (PFTs). Ten PFTs were identified. Two trade-offs were observed: resource acquisition vs. resource conservation or resource assignment to tissues conferring anoxia or salinity tolerance, and fast growth vs. light interception. Cover-abundance of the PFTs and community-level weighted means (CWMs) of the non-redundant traits were computed for 80 sites. Generalized linear models were used to assess the effect of environmental variables (landscape units, topographic positions, flooding frequency and eight soil variables) on the CWMs and on distribution of PFTs occurring in 15 sites or more. A two-stage modelling process was carried out to account for the presence?absence and cover?abundance of PFTs separately. The association between CWMs and environmental features was relatively weak but significant for six out of the eight traits analysed. Besides, satisfactory models were found for eight out of the nine PFTs assessed. In the studied floodplain, PFTs &#8722; interpreted in terms of life strategies &#8722; seem to be a better approach than CWMs to understand and characterise functional responses to the environment.","palabras_clave":"Assembly rules|Generalized linear models|Functional traits|Life strategy|Marshes|Wetlands"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6371,"idioma_id":4,"titulo":"Mapping plant functional types in floodplain wetlands: an analysis of C-band polarimetric SAR data from RADARSAT-2","resumen":"The inclusion of functional approaches on wetland characterizations and on biodiversity assessments improves our understanding of ecosystem functioning. In the Lower Paraná River floodplain, we assessed the ability of C-band polarimetric SAR data of contrasting incidence angles to discriminate wetland areas dominated by different plant functional types (PFTs). Unsupervised H\/&#945; and H\/A\/&#945; Wishart classifications were implemented on two RADARSAT-2 images differing in their incidence angles (FQ24 and FQ08). Obtained classes were assigned to the information classes (open water, bare soil and PFTs) by a priori labeling criteria that involved the expected interaction mechanisms between SAR signal and PFTs as well as the relative values of H and &#945;. The product obtained with the shallow incidence angle scene had a higher accuracy than the one obtained with the steep incidence angle product (61.5% vs. 46.2%). We show how a systematic analysis of the H\/A\/&#945; space can be used to improve the knowledge about the radar polarimetric response of herbaceous vegetation. The map obtained provides novel ecologically relevant information about plant strategies dominating the floodplain. Since the obtained classes can be interpreted in terms of their functional features, the approach is a valuable tool for predicting vegetation response to floods, anthropic impacts and climate change.","palabras_clave":"INCIDENCE ANGLE|MACROPHYTES|MARSHES|SAR POLARIMETRY|POLARIMETRIC DECOMPOSITIONS|WISHART CLASSIFICATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6372,"idioma_id":5,"titulo":"Factores de riesgo, representaciones y prácticas asociadas con la leishmaniasis visceral humana en un foco urbano emergente en Posadas, Argentina","resumen":"Introducción. La leishmaniasis visceral es una enfermedad desatendida de alta letalidad que amerita que se profundice en los estudios locales para diseñar estrategias efectivas de control. En América, su transmisión ya se ha evidenciado en las zonas urbanas, en donde el reservorio principal es el perro doméstico. Las condiciones socioeconómicas, así como las representaciones y las prácticas de los grupos domésticos podrían desempeñar un papel crucial y poco conocido en su transmisión.Objetivo. Analizar las representaciones y las prácticas de prevención y afrontamiento de la leishmaniasis visceral humana y canina, así como los factores socioeconómicos de riesgo.Materiales y métodos. Se hizo un estudio transversal de casos y controles. Se definió ?caso? como un grupo doméstico en el que, al menos, un integrante tuviera antecedentes de leishmaniasis visceral humana y hubiera sido notificado por el Ministerio de Salud Pública. El ?control? se definió como un grupo doméstico sin integrantes con antecedentes de la enfermedad. A partir de la información primaria, se caracterizó demográfica y socialmente a la población, se estableció la calidad de las viviendas, se hizo una clasificación de los conocimientos y las actitudes en torno a la enfermedad, y se describieron las prácticas asociadas al riesgo y la presencia de leishmaniasis visceral canina.Resultados. La mala calidad de la vivienda (p&#8804;0,001), las personas del grupo doméstico que permanecían fuera de esta después de las 18 horas (razón de momios, OR (odds ratio)=4,5; IC95% 1,69-12,18), la reproducción sin control de la raza canina (OR=15,7; IC95% 3,91-63,2) y la presencia de leishmaniasis visceral canina en el domicilio (OR=120,3; IC 95% 18,51-728,3), se asociaron positivamente o incrementaron el riesgo de contraer leishmaniasis visceral humana.Conclusión. Se observó que la pobreza estructural constituía un factor social determinante del riesgo, y que aumentaba la probabilidad de contacto entre humanos y vectores por la mala calidad de la vivienda y el hacinamiento. El factor de riesgo más importante para la leishmaniasis visceral humana fue la tenencia en el domicilio de perros con la enfermedad.","palabras_clave":"LEISHMANIASIS VISCERAL|FACTORES DE RIESGO|CONOCIMIENTOS, ACTITUDES Y PRACTICAS|ENFERMEDADES DESATENDIDAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6373,"idioma_id":5,"titulo":"Volátiles de aceite esencial de romero logran una mejor calidad de tomate fresco cortado almacenado en atmósfera modificada","resumen":"Los aceites esenciales de plantas y especias poseen un reconocido efecto antimicrobiano y se los emplea como preservadores naturales de alimentos. El objetivo de este trabajo fue estudiar el efecto de la aplicación de aceite esencial (AE) en tomate fresco cortado almacenado en atmósfera modificada y refrigerado. Se realizaron ensayos con tomates cortados en rodajas (7 mm), colocando 6 rodajas por bandeja y aceites esenciales (AE) de romero o laurel (0, 15, 30, 50 y 100 &#956;L) en un papel de filtro sobre un lateral de la bandeja y se envasaron con film PD 961 (Cryovac®) para almacenarlos a 5°C por 13 d. Los respectivos controles no se envasaron con AE. El AE de romero (30 y 50 &#956;L) y laurel (30 &#956;L) mostraron un menor índice de deterioro respecto de los controles,aunque el más efectivo fue el AE de romero (30 &#956;L). Esta condición se empleó en un segundo ensayo en cual se identificó (por CG-MS) la presencia de los compuestos volátiles &#945;-pineno, canfeno, p-cimeno, 1,8-cineol y Lcanfor en el AE de romero empleado. Mientras que, luego de 4 d de almacenamiento, el 1,8-cineol y L-canfor fueron los compuestos mayoritarios en la atmósfera de las bandejas tratadas y los únicos presentes en las rodajas de tomate. Por otro lado, el empleo de AE de romero (30 &#956;L) retrasó el ablandamiento y desarrollo de bacterias, hongos y levaduras. Se aisló un hongo filamentoso y se identificó mediante claves taxonómicas como Aspergillus fumigatus y dos levaduras que se identificaron como Candida guilliermondii y Kloeckera spp. Mediante el kit API® 20C AUX. Se analizaron distintos volúmenes de AE frente a una determinada concentración de microorganismos, hallándose que dichos % de inhibición se incrementaban con la cantidad de AE. Los resultados muestran que el AE de romero ejerce un efecto antimicrobiano en diferentes volúmenes para A. Fumigatus, Candida guilliermondii y Kloeckera spp., y permite incrementar el tiempo de vida útil del tomate fresco cortado almacenado en atmósfera modificada.","palabras_clave":"1,8-CINEOL|L-CANFOR|ASPERGILLUS FUMIGATUS|CANDIDA GUILLERMONDII|KLOECKERA SPP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6374,"idioma_id":4,"titulo":"Preliminary characterization of dexamethasone-loaded cross-linked hyaluronic acid films for topical ocular therapy","resumen":"The aim of this work was to design and characterize cross-linked hyaluronic acid (HA)?itaconic acid (IT) &#64257;lms loaded with dexamethasone sodium phosphate salt (DEX) for topical therapy of in&#64258;ammatory ocular  surface diseases. Films were chemically cross-linked with polyethylene glycol diglycidyl ether (PEGDE),  then physical and mechanical characterization by stress?strain, X-ray diffraction, X-ray &#64258;uorescence  spectrometry and swelling assays was conducted. A sequential in vitro therapeutic ef&#64257;cacy model  was designed to assess changes in interleukin (IL)-6 production in an in&#64258;amed human corneal epithelial (HCE) cell line after &#64257;lm exposure. Changes in cell proliferation after &#64257;lm exposure were assessed using the alamarBlue proliferation assay. Experimental &#64257;ndings showed desirable mechanical properties  and in vitro ef&#64257;cacy to reduce cell in&#64258;ammation. A moderately decreased proliferation rate was induced in HCE cells by DEX-loaded &#64257;lms, compared to commercial DEX eye drops. These results suggest that DEX and HA have opposite effects. The sequential in vitro therapeutic ef&#64257;cacy model arises as an  ef&#64257;cient tool to study drug release from delivery systems by indirect measurement of a biological response.","palabras_clave":"Hyaluronan | Dexamethasone|Model Ocular In&#64258;ammation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6375,"idioma_id":4,"titulo":"GSTM1 and GSTP1, but not GSTT1 genetic polymorphisms are associated with chronic myeloid leukemia risk and treatment response","resumen":"Background: Chronic myeloid leukemia (CML) is associated to the BCR-ABL1 oncogene and can successfully be treated with tyrosine kinase inhibitors (TKIs). However, it remains still under investigation which molecular factors may influence CML risk or varying responses to TKIs. The aim of this study was to assess the role of Glutathione-S-transferases (GSTs) genetic polymorphisms in CML susceptibility and TKI clinical outcome.Materials: Deletion polymorphisms in GSTM1 and GSTT1 genes and the single nucleotide polymorphism in GSTP1 c.319A > G (rs1695; p.105Ile > Val) were genotyped by PCR methods in 141 CML treated patients and 141 sex- and age-matched healthy individuals.Results: Individual analysis of each GST gene showed no association with CML risk. A trend toward significance (p = 0.07) for a recessive model was found for GSTP1 (OR: 2.04; CI: 0.94-4.4). However, the combined analysis showed that GSTM1-null\/GSTP1-GG as well as GSTT1-null\/GSTP1-GG were associated with CML development (p = 0.03; OR: 3.54 CI: 1.2-14.57; p = 0.05; OR: 12.65; CI: 1.17-21.5). The relationship with treatment outcome showed that the presence of GSTM1 gene was significantly linked with an inferior rate of major molecular response (p = 0.048) and poor event free-survival (EFS) (p = 0.02).Furthermore, a group of patients with GSTP1-GG genotype were significantly associated with reduced EFS comparing to those carrying other GSTP1 genotypes (p = 0.049). GSTP1-GG genotypes had short time to treatment failure in a group of patients unresponsive to TKIs comparing to other GSTP1 genotypes (p = 0.03).Conclusions: This study highlights the significance of GSTM1 and GSTP1 polymorphisms on CML susceptibility and response to TKIs in the Argentinean population.","palabras_clave":"CHRONIC MYELOID LEUKEMIA |GLUTATHIONE-S-TRANSFERASES|PHARMACOGENETICS|LEUKEMIA SUSCEPTIBILITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6376,"idioma_id":4,"titulo":"Immature myeloid Gr-1+ CD11b+ cells from lipopolysaccharide-immunosuppressed mice acquire inhibitory activity in the bone marrow and migrate to lymph nodes to exert their suppressive function.","resumen":"Secondary infections due to post-sepsis immunosuppression are a major cause of death in patients with sepsis. Repetitive inoculation of increasing doses of lipopolysaccharide (LPS) into mice mimics the immunosuppression associated with sepsis. Myeloid-derived suppressor cells (MDSCs, Gr-1(+) CD11b(+)) are considered a major component of the immunosuppressive network, interfering with T-cell responses in many pathological conditions. We used LPS-immunosuppressed (IS) mice to address whether MDSCs acquired their suppressive ability in the bone marrow (BM) and whether they could migrate to lymph nodes (LNs) to exert their suppressive function. Our results showed that Gr-1(+) CD11b(+) cells of IS mice already had the potential to inhibit T-cell proliferation in the BM. Moreover, soluble factors present in the BM from IS mice were responsible for inducing this inhibitory ability in control BM cells. In addition, migration of Gr-1(+) CD11b(+) to LNs in vivo was maximal when cells obtained from the BM of IS mice were inoculated into an IS context. In this regard, we found chemoattractant activity in cell-free LN extracts (LNEs) from IS mice and an increased expression of the LN-homing chemokine receptor C-C chemokine receptor type 7 (CCR7) in IS BM Gr-1(+) CD11b(+) cells. These results indicate that Gr-1(+) CD11b(+) cells found in BM from IS mice acquire their suppressive activity in the same niche where they are generated, and migrate to LNs to exert their inhibitory role. A better understanding of MDSC generation and\/or regulation of factors able to induce their inhibitory function may provide new and more effective tools for the treatment of sepsis-associated immunosuppression.","palabras_clave":"modelo murino|Medula osea|mieloides supresoras|inmunosupresion por LPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6377,"idioma_id":4,"titulo":"A moveable feast: insects moving at the forest-crop interface are affected by crop phenology and the amount of forest in the landscape","resumen":"Edges have become prevailing habitats, mainly as a result of habitat fragmentation andagricultural expansion. The interchange of functionally relevant organisms like insectsoccurs through these edges and can influence ecosystem functioning in both crop and noncrophabitats. However, very few studies have focused on the directionality of insect movementthrough edges, and the role of crop and non-crop amount has been ignored. Using bidirectionalflight interception traps we investigated interchange of herbivore, natural enemy,pollinator and detritivore insects between native forest fragments and soybean crops, simultaneouslyconsidering movement direction, forest cover in the landscape and crop phenology.In total, 52,173 specimens and 877 morphospecies were collected. We found that,within most functional and taxonomic groups, movement intensity was similar (richnessand\/or abundance) between directions, whereas a predominantly forest-to-crop movementcharacterized natural enemies. Insect movement was extensively affected by crop phenology,decreasing during crop senescence, and was enhanced by forest cover particularly atsenescence. Mainly the same herbivore species moved to and from the forest, but differentnatural enemy species predominated in each direction. Finally, our analyses revealedgreater forest contribution to natural enemy than to herbivore communities in the crop, fadingwith distance to the forest in both groups. By showing that larger amounts of forest leadto richer insect interchange, in both directions and in four functional groups, our study suggeststhat allocation to natural and cultivated habitats at landscape level could influencefunctioning of both systems. Moreover, natural enemies seemed to benefit more than pestsfrom natural vegetation, with natural enemy spillover from forests likely contributing to pestcontrol in soybean fields. Thus consequences of insect interchange seem to be mostly positivefor the agroecosystem, although consequences for the natural system deserve furtherstudy.","palabras_clave":"INSECT MOVEMENT|INSECT GUILDS|CROP FOREST INTERFASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6378,"idioma_id":4,"titulo":"Trophic web associated with the South American tomato moth Tuta absoluta: Implications for its conservation biological control in Argentina","resumen":"1 The South American tomato moth Tuta absoluta is a major pest targeted for biological control by entomophagous insects. Trophic interactions among parasitoids of T.absoluta and other gelechiids feeding on crop and spontaneous wild solanaceous were investigated in north-eastern Buenos Aires (Argentina), with the aim of assisting in the design of conservation biological control strategies.2 A quantitative parasitoid trophic web was constructed that included five gelechiid leafminers, five solanaceous species and a complex of 18 parasitoid species.3 The relative abundance of T. absoluta was highest in the Solanum melongenacrop, followed by the wild plants Nicotiana glauca, Solanum americanum, Solanumsisymbriifolium and Salpichroa origanifolia. Greater parasitoid species richnessassociated with T. absoluta was found in the wild plants N. glauca and S. americanum.4 Pseudapanteles dignus was the main parasitoid of T. absoluta and co-occurred with this pest in four plant species.5 Parasitism of T. absoluta by P. dignus was observed throughout the year inS. melongena, reaching values up to 33%, whereas it was sporadic and exhibited lower parasitism rates in the noncrop S. sisymbriifolium, N. glauca and S. americanum.6 The presence of some wild solanaceous plants should be promoted to maintain the T. absoluta? P. dignus interaction in horticultural farm.","palabras_clave":"Agroecosystem  |Biocontrol|Community| Leafminer|Parasitic Hymenoptera,|Solanaceae,|Trophic interactions."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6379,"idioma_id":4,"titulo":"Natural vegetation cover in the landscape and edge effects: differential responses of insect orders in a fragmented forest","resumen":"Human activities have led to global simplification of ecosystems, among which Neotropical dry forests are some of the most threatened. The reduction of non-crop habitats in the landscape as well as edge effects may affect insect communities. Here, we analyzed insect communities sampled with broad spectrum pan traps in nine landscapes comprising cultivated fields and Chaco Serrano forests, at overall community and taxonomic order level. In total 7,043 specimens and 456 species of hexapods were captured, and we found that abundance and richness were directly related to forest cover and higher at edges in comparison with forest interior. Community composition also varied with forest cover and edge\/interior location. Different responses were detected among the eight dominant orders, albeit none responded negatively to forest cover or edge location. Collembola, Hemiptera and Orthoptera richness and\/or abundance were positively related to forest cover, while Thysanoptera abundance also increased with forest cover, but only at the edge. Coleoptera, Diptera and Hymenoptera were more diverse and abundant at the edge. The negative influence of forest loss on insect communities could have functional consequences for both natural and cultivated systems, and highlights the relevance of forest conservation. The higher diversity of insects at the edges could result from the simultaneous presence of forest and matrix species, although ?resource mapping? could be involved for orders that were richer and more abundant at edges. Adjacent crops could benefit from forest proximity since natural enemies and pollinators are well represented in the orders showing positive edge effects.","palabras_clave":"FOREST COVER|NATURAL ENEMIES|CHACO SERRANO FOREST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6380,"idioma_id":4,"titulo":"Trophic interactions between parasitoids and necrophagous flies in Central  Argentina","resumen":"The main exploiters of decay organic matter are larvae of necrophagous flies in the families Calliphoridae, Sarcophagidae and Muscidae. The knowledge of insect species associated with each stage of decay can be used to estimate the time since death in the crime scene. Dipteran larvae are attacked by a rich community of parasitoids, including species of Braconidae, Ichneumonidae and Pteromalidae (Hymenoptera: Parasitica). This study examined the parasitic complex associated with flies of forensic and sanitary importance, in the city of Córdoba (Argentina). During December to March (2012-2013) parasitoids were collected through traps with beef liver as bait for flies, in two sites with different urbanization levels. Rates of parasitism and parasitized pupae were estimated and species composition analyzed for both communities. Sarcophagidae was the most abundant family, represented by two species, followed by Calliphoridae. Nasonia vitripennis Ashmead (Hymenoptera) was the most abundant species and was collected in a wider variety of hosts. To the best of our knowledge, this is the first study providing accurate information about trophic interactions between calyptrate dipteran species and their hymenopterans parasitoids in the central region of Argentina.","palabras_clave":"SINANTROPIC FLIES|PARASITOIDS|TROPHIC INTERACTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6381,"idioma_id":4,"titulo":"Progress in bacterial cellulose matrices for biotechnological applications","resumen":"Bacterial cellulose (BC) is an extracellular polymer produced by many microorganisms. The Komagataeibacter genus is the best producer using semi-synthetic media and agricultural wastes. The main advantages of BC are the nanoporous structure, high water content and free hydroxyl groups. Modification of BC can be made by two strategies: in-situ, during the BC production, and ex-situ after BC purification. In bioprocesses, multilayer BC nanocomposites can contain biocatalysts designed to be suitable for outside to inside cell activities. These nanocomposites biocatalysts can (i) increase productivity in bioreactors and bioprocessing, (ii) provide cell activities does not possess without DNA cloning and (iii) provide novel nano-carriers for cell inside activity and bioprocessing. In nanomedicine, BC matrices containing therapeutic molecules can be used for pathologies like skin burns, and implantable therapeutic devices. In nanoelectronics, semiconductors BC-based using salts and synthetic polymers brings novel films showing excellent optical and photochemical properties.","palabras_clave":"Bacterial cellulose|Biocatalysis|Biocomposites|Bioprocessing|Fermentation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6382,"idioma_id":4,"titulo":"Does network complexity help organize Babel?s library?","resumen":"In this work we show that global topological properties of co?ocurrent word networks constructed from texts, seem to be the fingerprint of meaningful sentences. We observe that many statistical properties of these networks depend on the frequency of words, however, others seem to be strictly determined by the grammar. Our results suggest that seems to be a lower bound of sense that depends on the correlation between mean word connectivity and word connectivity correlation. This property, in addition to being only present in meaningful texts, and absent in, until now, not decoded texts such as the Voynich manuscript, would also be exclusive for natural languages, allowing us to discriminate between these and formal texts.","palabras_clave":"Voynich manuscript|complex networks|word networks|quantitative linguistics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6383,"idioma_id":4,"titulo":"Cities and Methods from Complexity Science","resumen":"The dynamics of human society is now been studying in the context of the artificial environment created by cities. In this work, the authors describe some of the formal methods used in complexity science to study urban systems. The authors discuss some of the important quantitative approaches on cities paying attention to some of the deepest controversies in present scientific studies. The authors will stress the importance of a transdisciplinary approach when studying this type of cooperative social environments.","palabras_clave":"cities|complexity|envioroment|complex systems"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6384,"idioma_id":4,"titulo":"Draft genome sequence of cellulolytic and xylanolytic Cellulomonas sp. B6 strain isolated from sub-tropical forest soil","resumen":"Cellulomonas sp. strain B6 was isolated from a subtropical forest soil sample and presented (hemi)cellulose-degrading activity. We report                     here its draft genome sequence, with an estimated genome size of 4&nbsp;Mb, a G+C content of 75.1%, and 3,443 predicted protein-coding                     sequences, 92 of which are glycosyl hydrolases involved in polysaccharide degradation.","palabras_clave":"Genome|cellulolytic|forest"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6385,"idioma_id":4,"titulo":"Analyses of apoptosis and DNA damage in bovine cumulus cells after in vitro maturation with different copper concentrations: consequences on early embryo development","resumen":"The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg\/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus-oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg\/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu. Cumulus expansion and viability of CC did not show differences in COC treated with 0, 20, 40, or 60 µg\/dl Cu during IVM. After in vitro fertilization (IVF), cleavage rates were higher in COC and DO + CC (denuded oocytes + CC) with or without Cu than in DO. Independently of CC presence (COC, DO + CC or DO) the blastocyst rates were higher when 60 µg\/dl Cu was added to IVM medium compared to medium alone. These results indicate that Cu supplementation to IVM medium: (i) decreased DNA damage and apoptosis in CC; (ii) did not modify oocyte viability and cumulus expansion; and (iii) improved subsequent embryo development up to blastocyst stage regardless of CC presence during IVM.","palabras_clave":"Apoptosis|DNA damage|Early development|Minerals"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6386,"idioma_id":5,"titulo":"Intersecciones: espacio físico, social y mediático en la construcción cotidiana de una 'ciudad ordinaria' en San Salvador de Jujuy, Argentina","resumen":"El espacio mediático resulta co-constitutivo del espacio social - y de sus vínculos con el espacio físico-  ya que no se limita a dar forma a algo previo y ya existente sino que es parte del proceso de formación, estabilización, crítica o ruptura de la mayor parte de las figuras que se presentan al debate social. Este trabajo propone indagar en la conflictividad de lo social expresada en imágenes, relatos y prácticas de construcción del espacio de la ciudad de San Salvador de Jujuy. El abordaje plantea una atención transversal a los procesos de producción de significaciones en torno a la ciudad y lo urbano en diferentes repertorios, a los procesos de territorialización realizados por actores de las organizaciones barriales Tupac Amaru y Tupaj Katari, y a la vinculación de ambos con la historia local.","palabras_clave":"Geografías del poder|Sentidos de ciudad|Medios hegemónicos|Movimientos Sociales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6387,"idioma_id":5,"titulo":"REINTERPRETANDO EL REGISTRO ARQUEOFAUNÍSTICO DEL FUERTE BLANCA GRANDE: \u000BAPROVECHAMIENTO DE DIFERENTES RECURSOS DURANTE EL SIGLO XIX (OLAVARRÍA, PROVINCIA DE BUENOS AIRES)","resumen":"En este trabajo se reinterpreta el consumo de animales que practicaron los habitantes del Fuerte Blanca Grande, ubicado en una antigua rastrillada, al sur del Río Salado (Provincia de Buenos Aires), desde 1800 hasta la década de 1880. La muestra faunística proviene de las diferentes áreas de descarte dando a conocer los cambios de consumo de especies silvestres y domesticas en este período. Teniendo en cuenta los cambios vinculados a las situaciones específicas de conflicto e intercambio en relación a la población originaria; la incorporación paulatina de terratenientes, hacendados, pulperos y colonos. Durante este proceso, la nueva población introdujo fauna, flora artefactos y costumbres vinculada a las explotaciones agropecuarias (cultivos, distintas especies de aves de corral, ganado vacuno y ovino) y disminuyendo la población de algunas especies autóctonas en zonas intensamente pobladas (e.g. Ozotoceros b. celer, entre otras). De esta manera, se espera que el registro arqueofaunístico varíe a lo largo del tiempo, teniendo en cuenta las características de los diferentes periodos del lugar, en relación con los asentamientos eurocriollos o de grupos originarios, y las relaciones que estos establecían con las diferentes políticas que se desarrollaron durante finales del siglo XVIII y gran parte del siglo XIX.","palabras_clave":"FUERTE BLANCA GRANDE|CAMINO DE LOS INDIOS A SALINAS|FRONTERA |ARQUEOFAUNA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6388,"idioma_id":4,"titulo":"Tandem  duplication events in the expansion of the small heat shock protein gene family in Solanum lycopersicum (cv. Heinz 1706)","resumen":"In plants, fruit maturation and oxidative stress can induce small heat shock protein (sHSP) synthesis to maintain cellular homeostasis. Although the tomato reference genome was published in 2012, the actual number and functionality of sHSP genes remain unknown. Using a transcriptomic (RNA-seq) and evolutionary genomic approach, putative sHSP genes in the Solanum lycopersicum (cv. Heinz 1706) genome were investigated. A sHSP gene family of 33 members was established. Remarkably, roughly half of the members of this family can be explained by nine independent tandem duplication events that determined, evolutionarily, their functional fates. Within a mitochondrial class subfamily, only one duplicated member, Solyc08g078700, retained its ancestral chaperone function, while the others, Solyc08g078710 and Solyc08g078720, likely degenerated under neutrality and lack ancestral chaperone function. Functional conservation occurred within a cytosolic class I subfamily, whose four members, Solyc06g076570, Solyc06g076560, Solyc06g076540, and Solyc06g076520, support &#8764;57% of the total sHSP RNAm in the red ripe fruit. Subfunctionalization occurred within a new subfamily, whose two members, Solyc04g082720 and Solyc04g082740, show heterogeneous differential expression profiles during fruit ripening. These findings, involving the birth\/death of some genes or the preferential\/plastic expression of some others during fruit ripening, highlight the importance of tandem duplication events in the expansion of the sHSP gene family in the tomato genome. Despite its evolutionary diversity, the sHSP gene family in the tomato genome seems to be endowed with a core set of four homeostasis genes: Solyc05g014280, Solyc03g082420, Solyc11g020330, and Solyc06g076560, which appear to provide a baseline protection during both fruit ripening and heat shock stress in different tomato tissues.","palabras_clave":"sHSP  | ripening  |tomato  transcriptome  | RNA-seq  tandem duplication"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6389,"idioma_id":4,"titulo":"CHARACTERIZATION AND SELECTION OF MICROHABITAT BY Microcavia australis (RODENTIA: CAVIIDAE): FIRST DATA IN A ROCKY HABITAT IN THE HYPERARID MONTE DESERT OF ARGENTINA","resumen":"A rocky habitat, in its broadest sense, may be described as any locality that contains boulders, rocks, scree, pebbles, outcrops, cliffs, or caves. In these habitats, mammals find shelter sites that they use as nesting sites or dens to raise their young in a stable microclimate that is relatively secure from predators. The aim of this study was to characterize the habitat used by the Southern mountain cavy (Microcavia australis) in rock outcrops of the Monte desert of San Juan province, Argentina. The Southern mountain cavy occupied sites with high rock cover and low cover of vegetation and bare soil and used the crevices formed during a piping process. This rodent selected the highest and deepest crevices, which provided them with a more stable micro-environment than outside. The present study is the first to report a Southern mountain cavy population associated with a rocky habitat, suggesting a strong plasticity to inhabit a variety of habitats, as indicated by its wide distribution.","palabras_clave":"crevices|habitat characterization|Microcavia australis|Monte Desert; rock outcrops"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6390,"idioma_id":4,"titulo":"Trypanosoma cruzi Experimental Infection Impacts on the Thymic Regulatory T Cell Compartment","resumen":"The dynamics of regulatory T cells in the course of Trypanosoma cruzi infection is still debated. We previously demonstrated that acute murine T. cruzi infection results in an impaired peripheral CD4+Foxp3+ T cell differentiation due to the acquisition of an abnormal Th1-like phenotype and altered functional features, negatively impacting on the course of infection. Moreover, T. cruzi infection induces an intense thymic atrophy. As known, the thymus is the primary lymphoid organ in which thymic-derived regulatory T cells, known as tTregs, differentiate. Considering the lack of available data about the effect of T. cruzi infection upon tTregs, we examined tTreg dynamics during the course of disease. We confirmed that T. cruzi infection induces a marked loss of tTreg cell number associated to cell precursor exhaustion, partially avoided by glucocorticoid ablation- and IL-2 survival factor depletion. At the same time, tTregs accumulate within the CD4 single-positive compartment, exhibiting an increased Ki-67\/Annexin V ratio compared to controls. Moreover, tTregs enhance after the infection the expression of signature markers (CD25, CD62L and GITR) and they also display alterations in the expression of migration-associated molecules (&#945; chains of VLAs and chemokine receptors) such as functional fibronectin-driven migratory disturbance. Taken together, we provide data demonstrating profound alterations in tTreg compartment during acute murine T. cruzi infection, denoting that their homeostasis is significantly affected. The evident loss of tTreg cell number may compromise the composition of tTreg peripheral pool, and such sustained alteration over time may be partially related to the immune dysregulation observed in the chronic phase of the disease.","palabras_clave":"CD4+ Foxp3+ T cell|Trypanosoma cruzi|thymic atrophy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6391,"idioma_id":4,"titulo":"THE AMMONITE GENUS GAUDRYCERAS FROM THE SANTONIAN-CAMPANIAN OF ANTARCTICA: SYSTEMATICS AND BIOSTRATIGRAPHY","resumen":"Belying previous interpretations on its rarity in southern latitudes, Gaudryceras de Grossouvre is well represented in the Santonian?Campanian of Antarctica. The study of more than 300 specimens from the Santa Marta, Rabot and Snow Hill Island Formations designates fivespecies that characterize four successive stratigraphic intervals: 1) Gaudryceras cf. G. strictum, Santonian, Alpha Member, lower part of the SantaMarta Formation; 2) Gaudryceras santamartense sp. nov., Santonian?early Campanian, Alpha Member, lower mid part of the Santa Marta Formation;3) Gaudryceras brandyense sp. nov., late early Campanian??basal mid Campanian, Beta Member, upper part of the Santa Marta Formation;and 4) Gaudryceras cf. G. mite and Gaudryceras rabotense sp. nov., mid Campanian?late Campanian, upper middle part of the Rabot Formationand lower part of the Hamilton Point Member of the Snow Hill Island Formation. There are two differently ornamented neanoconchs characterizedby exhibiting dense or sparse strong flares, which are features of taxonomic importance at the specific level. In Antarctica, Gaudrycerasis well represented from the Santonian to the mid Campanian, rare in the late Campanian and not known in the Maastrichtian. Such facts stronglycontrast with data recorded outside Antarctica, particularly in the North Pacific, where Gaudryceras is well represented up to the late Maastrichtian.A striking biogeographical contrast is apparent for the Antarctic gaudryceratids. That is, while the early Campanian Gaudrycerasbrandyense sp. nov. and the mid?late Campanian G. rabotense sp. nov. are apparently restricted to Antarctica, the Santonian?early CampanianAntarctic species are very similar or co-specific to widely distributed forms.","palabras_clave":"ANTARCTICA|LYTOCERATIDS|GAUDRYCERAS|UPPER CRETACEOUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6392,"idioma_id":5,"titulo":"Predicciones precisas de marea astronómica calculadas a partir de altimetría satelital y observa-ciones costeras para la zona de Isla Grande de Tierra del Fuego, Islas de los Estados y Canal de Beagle","resumen":"La marea oceánica constituye una de las principales contribuciones a la variación de la altura de la superficie del mar, siendo la responsable de gran parte de la varianza en muchas variables oceanográficas. También, es indispensable disponer de predicciones precisas de marea para muchas aplicaciones prácticas en el medioambiente marino, como por ejemplo, la navegación, planificación de construcciones costeras, determinación de zonas inundables y el modelado de la dispersión de contaminantes. En las plataformas continentales la marea puede ser forzada fundamentalmente por fenómenos de resonancia producidos por la co-oscilación de la marea oceánica, alcanzando grandes amplitudes. Un ejemplo de fenómenos de resonancia corresponde a la plataforma continental donde se encuentra la costa Este de la Isla Grande de Tierra del Fuego donde las amplitudes de marea varían entre los 4 m y 10 m, para luego disminuir en el canal de Beagle a 2 m. Esta zona ha sido desde siempre un área de interés debido a las importantes reservas de hidrocarburos que posee, requiriendo disponer depredicciones de marea muy precisas tanto para las tareas de apoyo logístico como para las propias de explotación. Una manera alternativa para calcular predicciones de marea astronómica es utilizar constantes armónicas provistas por los resultados de modelos globales como GOT4.7, TPXO7.2, TPXO8, FES2012, EOT11a y regionales como AO-ATLAS-2011, disponibles en INTERNET. Aunque estos modelos mejoraron sustantivamente el conocimiento de la marea a escala global, sus resultados, en las cercanías de las costas son menos precisos que en el resto de los océanos, teniendo además escasa resolución (entre 3,7 km x 3,7 km y 18,5 km x18,5 km). El objetivo de este trabajo es obtener constantes armónicas de marea para una grilla de 500 m x 500 m, comprendida entre las latitudes 52°00?00? S y 55°20?30? S y las longitudes 63°30?00? O y 69°00?00? O, que resulten suficientes para realizar predicciones precisas de marea astronómica. A partir de estos resultados se construyen cartas cotidales y de isoamplitudes para lograr una adecuada y completa caracterización de la marea astronómica en la región. Como información de base se utilizan alturas del nivel del mar obtenidas en 34 estaciones de marea y 111 posiciones con información de altimetría satelital para un área que se extiende un poco más allá de la zona de estudio. Los resultados obtenidos se verifican realizando regresiones lineales entre alturas de marea predichas con las constantes armónicas calculadas y alturas del nivel del mar obtenidas con altimetría satelital, correspondientes a 10 posiciones distintas de las 111 utilizadas para calcular la grilla, obteniéndose pendientes próximas a 1. Además para 3 localidades se calcularon los RMSMisfit entre las constantes armónicas aquí obtenidas y las calculadas por Richter et al. (2012), obteniendo valores en el rango 0,00 m y 0,04 m para las componentes que no estaban contaminadas por otras de frecuencias cercanas. Estos resultados indican que las constantes armónicas obtenidas permiten calcular predicciones de marea astronómica precisas. Finalmente, utilizando la grilla de constantes armónicas obtenidas, se calcula la superficie correspondiente a la marea astronómica del 11\/08\/2014 a las 9 horas (huso horario +3), para toda la zona de estudio.","palabras_clave":"constantes armónicas de marea|cartas cotidales|cartas de isoamplitudes|altimetría satelital|Tierra del Fuego."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6393,"idioma_id":4,"titulo":"Glypican-3 induces a mesenchymal to epithelial transition in human breast cancer cells","resumen":"Breast cancer is the disease with the highest impact on global health, beingmetastasis the main cause of death. To metastasize, carcinoma cells must reactivate a latent program called epithelial-mesenchymal transition (EMT), through which epithelial cancer cells acquire mesenchymal-like traits.Glypican-3 (GPC3), a proteoglycan involved in the regulation of proliferationand survival, has been associated with cancer. In this study we observed thatthe expression of GPC3 is opposite to the invasive\/metastatic ability of Hs578T,MDA-MB231, ZR-75-1 and MCF-7 human breast cancer cell lines. GPC3 silencingactivated growth, cell death resistance, migration, and invasive\/metastatic capacity of MCF-7 cancer cells, while GPC3 overexpression inhibited these properties in MDA-MB231 tumor cell line. Moreover, silencing of GPC3 deepened the MCF-7 breast cancer cells mesenchymal characteristics, decreasing the expression of the epithelial marker E-Cadherin. On the other side, GPC3 overexpression induced the mesenchymal-epithelial transition (MET) of MDA-MB231 breast cancer cells, which re-expressed E-Cadherin and reduced the expression of vimentin and N-Cadherin.While GPC3 inhibited the canonical Wnt\/&#946;-Catenin pathway in the breast cancer cells, this inhibition did not have effect on E-Cadherin expression. We demonstrated that the transcriptional repressor of E-Cadherin - ZEB1 - is upregulated in GPC3 silenced MCF-7 cells, while it is downregulated when GPC3 was overexpressed in MDA-MB231 cells.We presented experimental evidences showing that GPC3 induces the E-Cadherinre-expression in MDA-MB231 cells through the downregulation of ZEB1.Our data indicate that GPC3 is an important regulator of EMT in breast cancer,and a potential target for procedures against breast cancer metastasis","palabras_clave":"GLYPICAN 3|HUMAN BREAST CANCER METASTASIS|EMT|ZEB1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6394,"idioma_id":5,"titulo":"SiNaRaMe: El Primer Sistema Integrado de Radares Hidro-Meteorológicos de Latinoamérica","resumen":"p { text-indent: 0.36cm; margin-top: 0.11cm; margin-bottom: 0.21cm; direction: ltr; line-height: 120%; text-align: justify; }p.cjk {  }a:link {  }Pocospaíses en Latinoamérica poseen radares meteorológicos operativosy, menos aún, han logrado la integración de estos mediante una red,junto a otros instrumentos de sensado remoto para monitoreohidrometeorológico. Definitivamente esto constituye una meta de sumaimportancia para cualquier país; en especial para aquellos tanextensos como la Argentina. Por esta razón, en 2009, se inició enla República Argentina un proyecto denominado Sistema Nacional deRadares Meteorológicos (SiNaRaMe) cuya meta principal es lograr lacobertura completa del territorio nacional mediante la integración ymodernización de la dispersa infraestructura disponible, como asítambién desarrollando doce nuevos sistemas de radar de fabricaciónnacional (la serie de Radares Meteorológicos Argentinos, RMA).SiNaRaMe, actualmente siendo llevado a cabo con éxito, coloca a laArgentina como pionera en tecnologías para hidrometeorología, anivel regional. El presente trabajo describe este proyecto incluyendouno de los más importantes aspectos a considerar en la búsqueda deuna red de radares meteorológicos completamente funcional: suproceso de calibración.","palabras_clave":"Banda C|Calibración|Doble-polarización|Integración Institucional|Red de Radares Meteorológicos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6395,"idioma_id":4,"titulo":"Rapid formation of adventitious roots and partial ethylene sensitivity result in faster adaptation to flooding in the aerial roots (aer) mutant of tomato","resumen":"The frequency of extreme events such as droughts and floods has increased as a consequence of climate change. Many crops have not been improved to tolerate soil anoxia and, therefore, floods cause important economic losses. During submergence, Solanum lycopersicum exhibits three distinct responses which are adventitious root production, epinasty and aerenchyma formation. The development of a new adventitious root system is crucial as it can replace the original roots that succumb to the hypoxic environment. Aerial roots (aer) is a tomato mutant characterized by the presence of numerous adventitious root primordia along the hypocotyl and older internodes. In this work, we have analyzed the aer mutant behavior to flooding to determine whether preformed adventitious roots represent an adaptive advantage with respect to biomass accumulation. We have also examined other morphological and anatomical responses of aer plants to determine differential adaptations under flooding. Aer plants form an abundant adventitious root system faster than AC, which results in flooded plants accumulating as much biomass as non-flooded aer plants. It appears that, several ethylene-induced responses such as epinasty, hypertrophy, aerenchyma production, and apical hook formation are reduced in aer, suggesting a lower sensitivity of some tissues to ethylene. The E4 expression level, an ethylene-induced gene, confirmed this observation since E4 transcripts are less abundant in petioles and stems of ethylene-treated aer plants, coinciding with the tissues that presented a lower degree of morphological and\/or anatomical response. Evidence from the Never ripe mutant suggests a reduction in ethylene sensitivity could contribute to the attenuation of flooding effects. Therefore, our results indicate that the rapid formation of a new root system together with a reduction in ethylene sensitivity is responsible of a faster adaptation to flooding stress in the aer mutant.","palabras_clave":"Tomato|Flooding|Adventitious roots|Biomass accumulation|Hypertrophy|Porosity|Aerenchyma|Epinasty|Ethylene"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6397,"idioma_id":4,"titulo":"Application of quantitative structure-property relationship analysis to estimate the vapor pressure of pesticides","resumen":"In this study, QSPR models were developed using multiple linear regression (MLR) methods to predict the vapor pressure values of 162 pesticides. Several feature selection methods, namely the replacement method (RM), genetic algorithms (GA), stepwise regression (SR) and forward selection (FS),were used to select the most relevant molecular descriptors from a pool of variables. The optimum subset of molecular descriptors was used to build a QSPR model to estimate the vapor pressures of the selected pesticides. The Replacement Method improved the predictive ability of vapor pressures and was more reliable for the feature selection of these selected pesticides. The results provided satisfactory MLR models that had a satisfactory predictive ability, and will be important for predicting vapor pressure values for compounds with unknown values. This study may open new opportunities for designing and developing new pesticide.","palabras_clave":"Teoría QSPR|Pesticides|Vapor pressure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6398,"idioma_id":4,"titulo":"Gonadotropin-inhibitory hormone in the cichlid fish Cichlasoma dimerus: 1 structure, brain 2 distribution and differential effects on the secretion of gonadotropins and growth hormone.","resumen":"The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis hasbeen well-established in birds and mammals. However, its role in other vertebrates, such as the teleostfish, remains controversial. In this context, the present study aimed to evaluate whether GnIHmodulates the release of gonadotrophins and growth hormone (GH) in the cichlid fish Cichlasomadimerus. First, we partially sequenced the precursor polypeptide for GnIH and identified three putativeGnIH peptides. Next, we analysed the expression of this precursor polypeptide via a polymerase chainreaction in the reproductive axis of both sexes. We found a high expression of the polypeptide in thehypothalamus and gonads of males. Immunocytochemistry allowed the observation of GnIH-immunoreactivesomata in the nucleus posterioris periventricularis and the nucleus olfacto-retinalis, withno differences between the sexes. GnIH-immunoreactive fibres were present in all brain regions, witha high density in the nucleus lateralis tuberis and at both sides of the third ventricle. Finally, we performedin vitro studies on intact pituitary cultures to evaluate the effect of two doses (106M and108M) of synthetic C. dimerus (cd-) LPQRFa-1 and LPQRFa-2 on the release of gonadotrophins andGH. We observed that cd-LPQRFa-1 decreased b-luteinising hormone (LH) and b-follicle-stimulatinghormone (FSH) and also increased GH release to the culture medium. The release of b-FSH wasincreased only when it was stimulated with the higher cd-LPQRFa-2 dose. The results of the presentstudy indicate that cd-LPQRFa-1, the cichlid fish GnIH, inhibits b-LH and b-FSH release and stimulatesGH release in intact pituitary cultures of C. dimerus. The results also show that cd-LPQRF-2could act as an b-FSH-releasing factor in this fish species.","palabras_clave":"HORMONA INHIBIDORA DE GONADOTROFINAS|HORMONA DE CRECIMIENTO|HORMONA FOLÍCULO ESTIMULANTE|HORMONA LUTEINIZANTE|PECES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6400,"idioma_id":5,"titulo":"Caracterización y procedencia de materias primas para la producción de alfarería prehispánica en la Quebrada de Humahuaca (Provincia de Jujuy, República Argentina). FRX, ICP y petrografía de pastas","resumen":"Se presenta un avance en la investigación arqueométrica de la alfarería prehispánica de la Quebrada de Humahuaca, territorio ubicado en el extremo noroeste de Argentina. Se analizaron 72 muestras de cerámicas y arcillas por FRX e ICP en tres laboratorios distintos. La integración de los datos y su procesamiento estadístico permitió identificar 4 grupos con características químicas definidas. La petrografía de pastas aportó información extra que ayudó a corroborar la congruencia de los agrupamientos obtenidos. Los resultados permitieron relacionar arcillas con cerámicas, diferenciar químicamente las arcillas y cerámicas de dos sectores de la Quebrada de Humahuaca estudiados e identificar posibles evidencias de interacción.","palabras_clave":"ALFARERIA PREHISPANICA|QUEBRADA DE HUMAHAUCA|FRX|ICP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6401,"idioma_id":4,"titulo":"Acrylic  acid  plasma  polymerized poly(3-hydroxybutyrate)  membranes  for methanol\/MTBE  separation by pervaporation","resumen":"Plasma  polymerized acrylic  acid  (AA)  thin  &#64257;lms  on  poly(3 hydroxybutyrate)  (PHB)  membranes  were  evaluated  as  composite membranes  for  methanol\/methyl  tert-butyl  ether(MTBE)  separation  by  pervaporation  (PV).  Composite  membranes  were  prepared  in  an induc-tively  coupled  radiofrequency  plasma  reactor.  Experiments  were  performed  at  different powers  (7.2  to  29.2  W)  and  pressures,using  pure  AA  monomer,  as  well  as  AA\/air  and AA\/argon  mixtures.  Depositions  chemical  characteristics  were  determined  through  FTIRspectroscopy, hydrophilic character through contact angle measurements, and SEM images were also taken to analyze surface morphology. In PV measurements, membranes weremethanol  selective;  the  separation  factor  exceeded  that  of  pure  PHB  membranes  in  all cases.  Fluxes  were  lower  than  the  original  membrane  but  the  performance  separation  index(PSI)  was  always  higher.  Results  presented  in  terms  of  permeability  were  between  660  and 4045  barrer  for  methanol,  while  for  MTBE  they  were  markedly  lower,  between  10.5  and 57.7 barrer.  These  large  differences  in  permeabilities  caused  high selectivities  which  ranged from  24  to  79.","palabras_clave":"ACRYLIC ACID|MEMBRANES|PERVAPORATION|PLASMA POLYMERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6402,"idioma_id":5,"titulo":"Cultivos de Cobertura Otoño-invernales y su relación con la Intercepción de la Radiación Fotosintéticamente Activa y la Materia Seca Aérea de Malezas","resumen":"Los cultivos de cobertura (CC) aparecen como una interesante alternativa al uso del barbecho químico debido al uso que hacen de los recursos aéreos y\/o subterráneos y la posibilidad de interferir con el crecimiento de malezas invernales.  El trabajo relaciona la materia seca aérea de cultivos de cobertura (CC), la materia seca aérea de malezas naturales y la intercepción de la radiación fotosintéticamente activa, de cara a establecer la potencial capacidad supresiva de malezas de los CC otoño-invernales.","palabras_clave":"Recursos aéreos |Interferencia|Manejo de malezas|Capacidad supresiva"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6403,"idioma_id":5,"titulo":"Historia de la formación de docentes para el Nivel Inicial en el Chaco: la conformación de la carrera de Profesorado de Educación Pre-elemental en la Universidad Nacional del Nordeste (1973-1982)","resumen":"La formación de docentes para el nivel inicial en el Chaco reconoce un recorridoque incluye preocupaciones provinciales y nacionales, plasmadas en propuestasformativas en las décadas de los ?60 y ?70 que dan cuenta de perspectivas teóricas diferenciadas que, aparentemente, han convivido de manera ?armónica? en la definición de lo que hoy conocemos como Profesorado de Educación Inicial.Nos interesa indagar acerca de ¿qué características adquirió la formación docente para el nivel pre-elemental en el contexto chaqueño de los ´70?, ¿qué tensiones existieron en la construcción identitaria de la formación de docentes para el nivel en el Chaco? A partir de ello describimos los actores e instituciones intervinientes, los sentidos y significados dados a la educación infantil en sus orígenes, el rol docente, entre otros, para aportar a la construcción de conocimientos historiográficos desde una perspectiva regional. Principalmente, la trayectoria de formación y accionar de Nélida Sosio de Iturrrioz nos resultaron relevantes para comprender los sentidos fundacionalesen la creación de la carrera de formación docente para el nivel.Recurrimos a diversas fuentes primarias tales como entrevistas semi estructuradas a docentes y ex alumnas de la carrera, documentos institucionales e informes de época sobre el nivel en el Chaco.","palabras_clave":"INICIAL|FORMACION|DOCENTE|CHACO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6404,"idioma_id":5,"titulo":"Entre lo público y lo privado: empleadores y trabajadoras domésticas frente al Tribunal del Trabajo Doméstico de la ciudad de Buenos Aires","resumen":"El servicio doméstico ocupa un lugar ambiguo entre los mundos público y privado. Desarrollado en el interior de los hogares de los empleadores, da lugar a relaciones en las que lo laboral y lo afectivo están imbricados. Los juicios laborales entre empleadores y trabajadoras domésticas constituyen un escenario privilegiado para observar el solapamiento de estas dimensiones. Si las demandas de las trabajadoras frente a las instituciones de justicia sitúan esta relación en el mundo público, las respuestas de los empleadores muchas veces buscan resituarlas en el orden privado. Por otra parte, en algunos escenarios, las demandas de las trabajadoras son también expresadas en un lenguaje que remite a lo privado. En este artículo analizamos las lógicas de la conflictividad judicial establecidas en las estrategias de empleadores y trabajadoras frente al Tribunal del Trabajo Doméstico (TTD), un organismo creado en 1956 para atender los conflictos individuales que derivan de las relaciones de trabajo de este sector en la ciudad de Buenos Aires. Tomamos dos horizontes temporales caracterizados por cambios en la regulación del trabajo, en general, y del servicio doméstico, en particular: el de los primeros años de funcionamiento del TTD y el cambio de siglo.","palabras_clave":"SERVICIO DOMÉSTICO|DERECHOS LABORALES|AFECTIVIDAD|PRIVADO\/ PÚBLICO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6405,"idioma_id":5,"titulo":"El movimiento de Mediación en Argentina: procesos, tensiones y afirmaciones","resumen":"El presente artículo busca contribuir, desde una perspectiva antropológica, al estudio de la Mediación como sensibilidad legal particular y forma específica de administración de conflictos. Se describe en primer término, a partir de fuentes documentales, entrevistas y textos especializados, los rasgos más sobresalientes del proceso de conformación de este campo en Argentina, que involucra un conjunto heterogéneo y a la vez poroso de actores judiciales, políticos, fundaciones, organismos internacionales. En un segundo momento, a partir de presentar un recorte de dos indagaciones etnográficas sobre experiencias de mediación concretas en escenarios locales, se muestra la diversidad de formas que ésta puede asumir, dando cuenta de la heterogeneidad de procedimientos, conceptos y criterios de tratamiento del conflicto, y lo particular y común que rodea al concepto de mediación comunitaria y penal. En tanto campo, la mediación se afirma a partir de los esfuerzos de actores institucionales concretos en su trabajo cotidiano de oponerse y diferenciarse de otras concepciones y universos institucionales, y sin embargo lo que observamos es la gran imbricación y porosidad entre informalidad y burocratización, mediación y administración, lo que desborda lo judicial y lo que queda atrapado allí.","palabras_clave":"mediación de conflictos;| campo; |sensibilidad legal; |administración de conflictos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6406,"idioma_id":4,"titulo":"Valeriana gaimanensis (Valerianaceae nom. conserv.), a New Species from the Patagonian Semi-arid Desert, Argentina","resumen":"Valeriana gaimanensis, a new species from Argentinean Patagonia, is described and illustrated. The new species belongs to the subgenus Valeriana and is apparently endemic to the Extra-Andean Patagonia region, where it grows in an extremelyarid environment, in contrast to most Valeriana species distributed in Patagonia. The morphological relationships of V. gaimanensis are discussed in comparison with V. carnosa, which resembles the new species morphologically. Principal component analysis was performedwith 28 morphological variables, including specimens of these two species, revealing differences between them. This study provides the distribution, habitat, phenology, and etymology of V. gaimanensis, as well as a key separating it from the other Valeriana species thatinhabit Extra-Andean Patagonia.","palabras_clave":"Chubut|Extra-Andean Patagonia|endemism|morphometric analyses"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6407,"idioma_id":4,"titulo":"A Locally Optimal Soft Linear-Quadratic Scheme for CR Systems in Shadowing Environments","resumen":"In this letter, we analyze the problem of detecting spectrum holes in cognitive radio systems under the Neyman-Pearson scenario. We consider that a group of unlicensed users use non-coherent energy detectors to sense the radio signal and design a soft locally optimal linear-quadratic statistic based on the deflection coefficient. Each unlicensed user transmits the processed data to a central entity, where the decision about the presence or not of licensed users is made. Using the method of Monte Carlo, we show that the proposed statistic outperforms previous ones available in the literature in a wide range of shadow-fading scenarios and it is robust against parameters errors.","palabras_clave":"Distributed detection|Cognitive radio|Spectrum sensing|Wireless sensor networks"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6408,"idioma_id":4,"titulo":"Bioproduction of floxuridine using nanostabilized biocatalysts","resumen":"Bionanocomposites employing natural polysaccharides such as alginate and nanoclays,e.g., bentonite, are a promising alternative to developing stabilized biocatalystsused in the pharmaceutical industry due to their physicochemical properties,biocompatibility, and nontoxicity. Mechanical parameters such as swelling ratio,compressive strength, and fracture frequency were optimized, favoring scale-up.Moreover, storage stability and reusability of the biocatalysts were improved bymore than 90% compared with control conditions. In addition, immobilized lacticacid bacteria were used in the bioprocess scale-up to obtain floxuridine, showing ahigh productivity per gram of biocatalyst.","palabras_clave":"Bioreactor|Biotransformations|Immobilization|Nanoparticles"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6409,"idioma_id":4,"titulo":"Impact of heat on seed germination of three perennial grasses in the semiarid region in Central Argentina","resumen":"Fire is an important factor affecting the viability and germination of seeds in the soil seed bank. We evaluated the effects of heat on germination and seed viability of three native perennials winter grasses of central Argentina. Seeds of two palatable grasses Piptochaetium napostaense (Speg.) Hack, Nassella tenuis (Phil.) Barkworth and a non-palatable Jarava ichu Ruiz & Pav. were exposed to different heat shock treatments resulting from a combination of temperature: 70, 90, 120, 140 and 170 C and three exposure times (5, 10 or 20 min) and a control (noheat) treatment. All control treatments had a high percentage of seed viability (>97%). Germination of P. napostaense was not affected by mild heat shock (70 and 90 C) but it was sensitive to higher temperatures: no seeds survived the exposure to 140 and 170 C. Seeds of this species have awns that facilitate the burring of seeds in the soil, which can reduce fire exposure. N. tenuis and J. ichu were less sensitive to heat. High heat shock reduced seed germination but not seed viability. These  experiments show that seeds of P. napostaense, the target species for management on these grasslands, were the most sensitive to heat  treatments. Our results show that heat shock can have a negative effect on the soil seed bank of P. napostaense (palatable and dominant species), but does not have significant effects on J. ichu seeds (unpalatable species). Thus, we suggest that the current management recommendations for the use of controlled fire to promote palatable species may not be optimal for these species.","palabras_clave":"grasslands|fire ecology|Poaceae"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6410,"idioma_id":4,"titulo":"Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis","resumen":"Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over &gt;99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7&#8201;kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis.","palabras_clave":"regulatory proteins| Mycobacterium bovis|polymophisms"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6411,"idioma_id":4,"titulo":"Breeding biology of Magellanic Penguins (Spheniscus magellanicus) at the Beagle Channel: interannual variation and its relationship with foraging behaviour","resumen":"Interannual variation in seabird foraging orreproductive behaviour may reflect fluctuations in marineresources. In this study, we evaluated different foragingand breeding parameters of Magellanic Penguins(Spheniscus magellanicus) from Martillo Island in theBeagle Channel, and the relationships between theseparameters at different stages within the season (incubation,early and late chick-rearing) over three consecutivebreeding seasons (2006?2007, 2007?2008 and 2008?2009).In 2007, we observed greater adult foraging effort (longerforaging trip duration and vertical travel distance, VTD)and lower chick feeding frequency, together with a slowergrowth rate and later fledging date of chicks, which wesuggest was linked to lower food availability near thecolony that year. The increased foraging effort appeared tobe compensated by enhanced feeding activity (e.g., numberof wiggles per dive). However, this increase did not coincidewith a larger amount of food load brought to colony,which may have been due to a change in the type or size ofprey consumed by the penguins. Magellanic Penguins fromMartillo Island showed great plasticity in foraging behaviour,as evidenced by changes in consumed prey type orincreased foraging effort and feeding activity when theconsumption of their main prey item, Sprattus fuegensis,seemed to decrease. Moreover, during this particularbreeding season, although the growth rate of chicks waslower, the breeding success remained constant throughoutthe study period, suggesting that the penguins managed tocompensate for the apparent decrease in Fuegian sprat.","palabras_clave":"Seabirds |Reproductive parameters |Foraging effort|Feeding activity|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6412,"idioma_id":4,"titulo":"Hybrid sterility between two populations of Diatraea saccharalis (Lepidoptera: Crambidae) (F.) from different Host Plant Species and Regions in Argentina","resumen":"The sugarcane borer, Diatraea saccharalis (F.), has a widespread distribution throughout the Western Hemisphere and is a pest of many crop plants including sUgarcane, corn, sorghum and rice. The use of Bacillus thuringiensis (Bt) corn has beenthe primary tool for managing this species in corn fields. Sugarcane borer control has been recently threatened by observations of susceptibility and\/or resistance to certain varieties of Bt corn and the protein used in many newer varieties. This has ledto increased interest in understanding sugarcane borer genetic diversity and gene flow within and among its populations and the consequent exchange of alleles between geographically distant populations. The objective of this study was to examine reproductive compatibility between host-associated geographic populations of D. saccharalis in Argentina and to determine whether this pest represents a complex of host-associated cryptic species rather than a wide ranging generalist Species. Intra and inter-population crosses revealed that D. saccharalis populations from the north-western and Pampas regions presented evidence of prezygotic and postzygotic incompatibility. Such a result is likely to be the product of an interruption of gene flow produced by either geographic or host plant associated isolation, suggesting that Tucumán (northwestern) and Buenos Aires (Pampas) populations of D. saccharalis are a distinct genotype and possibly an incipient species.","palabras_clave":"SUGARCANE BORER|FITNESS|REPRODUCTIVE COMPATIBILITY|CRYPTIC SPECIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6413,"idioma_id":4,"titulo":"Arbuscular mycorrhiza detoxifying response against arsenic and pathogenic fungus in soybean","resumen":"Uptake of Arsenic (As) in plant tissues can affect metabolism, causing physiological disorders, even death. As toxicity, but also pathogen infections trigger a generalised stress response called oxidative stress; however knowledge on the response of soybean (Glycine max L.) under multiple stressors is limited so far. Arbuscular mycorrhizal fungi (AMF) enhance the tolerance of host plants to abiotic and biotic stress. Thus, we investigated the effects of the AMF Rhizophagus intraradices on soybean grown in As-contaminated soils as well as in the presence of the pathogen Macrophomina phaseolina (charcoal rot of the stem). Plant parameters and degree of mycorrhizal colonization under the different assessed treatments were analysed. Content of As in roots and leaves was quantified. Increasing As level in the soil stopped plant growth, but promoted plant As uptake. Inoculation of soybean plants with M. phaseolina accentuated As effect at all physiological levels. In the presence of mycorrhizal symbiosis biomass dramatically increased, and significantly reduced the As concentration in plant tissues. Mycorrhization decreased oxidative damage in the presence of both As and the pathogen. Furthermore, transcription analysis revealed that the high-affinity phosphate transporter from R. intraradices RiPT and the gene encoding a putative arsenic efflux pump RiArsA were up-regulated under higher As doses. These results suggest that R. intraradices is most likely to get involved in the defense response against M. phaseolina, but also in the reduction of arsenate to arsenite as a possible detoxification mechanism in AMF associations in soybean.","palabras_clave":"Glycine max L|Rizhophagus intraradices|arsenic|Macrophomina phaseolina|oxidative stress"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6414,"idioma_id":4,"titulo":"Glyphosate Inhibits PPAR Gamma Induction and Differentiation of Preadipocytes and is able to Induce Oxidative Stress","resumen":"Glyphosate-based herbicides (GF) are extensively used for weed control. Thus, it is important to investigate their putative toxic effects. We have reported that GF at subagriculture concentrations inhibits proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts. In this investigation, we evaluated the effect of GF on genes upregulated during adipogenesis. GF was able to inhibit the induction of PPAR gamma, the master gene in adipogenesis but not C\/EBP beta, which precedes PPAR gamma activation. GF also inhibited differentiation and proliferation of another model of preadipocyte: mouse embryonic fibroblasts. In exponentially growing 3T3-L1 cells, GF increased lipid peroxidation and the activity of the antioxidant enzyme, superoxide dismutase. We also found that proliferation was inhibited with lower concentrations of GF when time of exposure was extended. Thus, GF was able to inhibit proliferation and differentiation of preadipocytes and to induce oxidative stress, which is indicative of its ability to alter cellular physiology.","palabras_clave":"3T3-L1 fibroblasts|adipogenesis|Glyphosate|oxidative stress"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6415,"idioma_id":4,"titulo":"N-Acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes","resumen":"Oxidative stress plays critical roles in the pathogenesis of diabetes, hypertension, and atherosclerosis; some authors reported that fat accumulation correlates to systemic oxidative stress in human and mice, but cellular redox environment effect on lipid accumulation is still unclear. In our laboratory we used mouse embryonic fibroblasts (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC) and accumulate lipids, as obesity model. Here we analyzed the role of the well-known antioxidant and glutathione precursor N-acetylcysteine (NAC) in cellular MAPK modulation and lipid accumulation. We evaluated the effect of NAC on the adipogenic differentiation pathway using different doses: 0.01, 0.1, 1 and 5mM; no toxic doses in these cells. A dose of 5mM NAC [DCN-5] provoked a significant decrease in triglyceride accumulation (72±10 [DCN-5] vs 169±15 [DC], p<0.01), as well in Oil Red O stained neutral lipid content (120±2 [DCN-5] vs 139±12 [DC], p<0.01). Molecular mechanisms responsible for adipogenic differentiation involve increase of the expression of phosphoERK½ and phosphoJNK, 5mM NAC treatment inhibited both pERK½ and pJNK protein levels. We also evaluated the mitotic clonal expansion (MCE) which takes place during adipogenesis and observed an increase in DC at a rate of 1.5 cells number compared to CC at day 2, whereas the highest doses of NAC significantly inhibited MCE. Our results suggest that NAC inhibits lipid accumulation and the MAPK phosphorylation in mouse embryonic fibroblasts during adipogenic differentiation and further contribute to probe the importance of cellular redox environment in adipogenesis.","palabras_clave":"ANTIOXIDANT|OBESITY|FIBROBLAST"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6416,"idioma_id":4,"titulo":"Glyphosate-based herbicides with different adjuvants are more potent inhibitors of 3T3-L1 fibroblast proliferation and differentiation to adipocytes than glyphosate alone","resumen":"Glyphosate-based herbicides are extensively used for weed control all over the world. Thus, it is important to investigate the putative toxic effects of these commercial formulations which contain not only glyphosate but also different adjuvants. 3T3-L1 fibroblasts are a useful tool in the study of adipocyte differentiation. We have previously reported that a commercial glyphosate formulation inhibits proliferation and differentiation in these cells. In the present investigation, we further evaluated the effect of different commercial glyphosate-based herbicides on 3T3-L1 fibroblast proliferation and differentiation and compared their effect with glyphosate itself. After treatment of 3T3-L1 fibroblasts with different concentrations of three glyphosate formulations or glyphosate itself, the increase in cell number or cytosolic lipid accumulation was determined. To evaluate the presence of polyethoxylated adjuvants, matrix-assisted laser desorption\/ionization (MALDI) analysis of the three commercial glyphosate-based herbicides was also performed. We found that the three commercial formulations tested were more potent inhibitors of both physiological processes: proliferation and differentiation to adipocytes of 3T3-L1 fibroblasts than glyphosate. We also found by MALDI analysis that the adjuvants were polyethoxylated in two of the formulations but not in the third one. It is well known the toxic effect of polyethoxylated adjuvants, but according to our results, non-polyethoxylated adjuvants may also contribute to the toxic effects. Our results further support the ability of glyphosate-based herbicides to disturb cellular physiology and highlight the importance of toxicological assessment of commercial formulations rather than glyphosate alone.","palabras_clave":"GLYPHOSATE-BASED HERBICIDES|GLYPHOSATE|3T3-L1 FIBROBLASTS|DIFFERENTIATION|PROLIFERATION|ADJUVANTS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6418,"idioma_id":4,"titulo":"SCLEROBIONTS, SHELL MORPHOLOGY AND BIOSTRATINOMY ON AMMONITES: TWO EARLY CRETACEOUS CASES FROM THE NEUQUÉN BASIN, ARGENTINA","resumen":"Some aspects of the paleobiology of ectocochleate cephalopods (e.g. floatability, life habit, biostratinomic routes) are still under discussion. Shell morphology affects these parameters, so it should impact the establishment and development of sclerobiont faunas as well. In this study, the sclerobiont faunas of Weavericeras vacaense (a spherocone) and Holchoptychites agrioensis (a discocone), two early Hauterivian ammonites from the Agrio Formation (Neuquén Basin, Argentina) were compared. A previous study case, the coeval nautilid Cymatoceras perstriatum, was contrasted with both ammonites. Results showed that the three sclerobiont faunas had similar abundance, taxonomic composition and distribution of individuals across the shells, but H. agrioensis showed a markedly reduced richness, with a fauna almost entirely composed by the oyster Amphidonte (Ceratostreon) sp. The more evolute and compressed H. agrioensis sunk more quickly than W. vacaense and C. perstriatum, which may have undergone longer periods of flotation and exposure on the sea bottom. This agrees with the lower taxonomic richness of the sclerobiont fauna and a better overall preservation of H. agrioensis specimens. Differences in the sclerobiont faunas are greater across variations in shell inflation and coiling degree than across nautilids and ammonites; therefore, the latter are important parameters for the sclerobiont fauna as well since they impact how long shells will float and be exposed on the seafloor, and therefore on their time of exposure. The presence of an almost monospecific fauna in H. agrioensis, despite its rapid sinking and burial, indicates that Amphidonte (Ceratostreon) sp. was the earliest settler, and could abundantly colonize hard substrates in a short time.","palabras_clave":"Hard sustrate fauna|Encrustation|Ammonite taphonomy|Necroplanktonic drift|Hauterivian|Agrio Formation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6419,"idioma_id":4,"titulo":"Development of the mosquito pathogen Leptolegnia  chapmanii (Straminipila: Peronosporomycetes) on an  inexpensive culture medium based on sunflower seed","resumen":"An inexpensive culture medium based on sunflower seed extract (SSE) for production of L. chapmanii was developed. Vegetative growth on solid and liquid SSE was compared with two culture media used routinely (PYG and Emerson YPss). Results indicate that the oomycete is able to grow on SSE medium, producing more zoospores at a faster rate as well as inducing higher mortality rates in Ae. aegypti larvae.","palabras_clave":"Mosquito control|diseases- borne vector|Entomopathogen|Straminipila"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6420,"idioma_id":5,"titulo":"La Salada: Imaginarios y representaciones de la informalidad y las desigualdades territoriales en la prensa escrita","resumen":"El objetivo general del presente trabajo es identificar y analizar los imaginarios y representaciones sociales de la informalidad y las desigualdades territoriales con un doble propósito: por una parte, para comprender el lugar que ocupan estos aspectos en la imagen de ciudad que configuran los medios de prensa gráficos a partir del tratamiento que hacen de la temática y por otra, para aportar elementos que contribuyan a reflexionar sobre las nuevas fronteras y espacios emergentes. Para ello se ha seleccionado el caso de la Feria La Salada ubicada en el conurbano bonaerense, donde la informalidad hace referencia a múltiples aspectos. En este sentido, se han relevado las representaciones en la prensa gráfica que permiten dar cuenta del imaginario dominante vinculado a la informalidad, las desigualdades territoriales y las valoraciones sobre los productos de la feria.","palabras_clave":"INFORMALIDAD URBANA|DESIGUALDADES TERRITORIALES|LA SALADA|IMAGINARIOS SOCIALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6421,"idioma_id":4,"titulo":"New Insight into the Interface Chemistry and Stability of Glutathione Self-Assembled Monolayers on Au (111)","resumen":"In this paper we have studied glutathione (GSH) self-assembled monolayers (SAMs) on Au(111) prepared by incubation from solutions in phosphate buffer (pH = 7). These SAMs degrade with increasing immersion time to yield adsorbed S and polysulfide species on the gold surface, as revealed by cyclic voltammetry and X-ray photoelectron spectroscopy. Results from polarization modulation infrared reflection?absorption spectroscopy (PM-IRRAS) show that even if GSH decomposes on the surface the vibrational bands characteristic of the molecule are still present. This has direct relevance for the study of gold nanoparticles (AuNPs) protected by hydrophilic thiols and also for biomedical applications of GSH-capped AuNPs. Thiol exchange experiments of the drug 6-mercaptopurine (6MP) SAMs on Au(111) in contact with GSH solutions were also performed to better understand the possible role of the latter in the triggered release of 6MP from the surface of AuNPs for drug delivery applications. Our results show that 6MP is completely released only when a S adlayer, produced by desulfurization of GSH, is formed on the Au(111) surface","palabras_clave":"Gold|glutation|sulfur|place exchange"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6422,"idioma_id":4,"titulo":"Revaluing the Tzanck Test: A Comparative Study with Direct Immunofluorescence for Herpes Virus","resumen":"Introduction: Tzanck Test (TT) is a sensitive method applicable to mucocutaneous lesions. Direct immunofluorescence (DIF) for Simplex Herpes Virus (HSV) is now a simple, no routine clinical laboratory practice, allows to differentiate the types of virus. Objectives: To evaluate the diagnostic sensitivity of Tzanck test vs DIF for herpetic lesions, and to know its usefulness as a complementary diagnostic tool to clinical skin lesions in patients with erosive, vesicular, bullous and pustular lesions. Materials and Methods: The TT was carried out in 157 lesion patient?s samples admitted to the Laboratory of Cytology of our Hospital from 1 January 2010 until 30 December 2014. Smears were stained with Giemsa and in parallel we performed DIF for HSV-1, HSV-2 and Varicella Zoster over 112 samples. Results: Of the total samples, 40 (25%) were unsatisfactory for cytodiagnosis. The TT was positive in 60 samples (38%), of which 55 (35%) showed cytopathic effect for Herpes virus, 2 (1.6%) samples showed characteristic inclusion bodies of Molluscum contagiosum infection (HendersonPaterson bodies), 1 (0.6%) sample showed acantholytic cells typical of Pemphigus Vulgaris, 1 (0.6%) showed morphological characteristics of Incontinentia Pigmenti and 1 (0.6%) presented immature cells of lymphoid appearance. 64 samples were positive by DIF and 58 by TT for HSV. Specificity and sensitivity for TT were 100% and 88% respectively. Conclusion: The TT should be appreciated as a useful tool in the diagnosis of skin lesions by the rapid implementation and their ease interpretation, as well as being affordable and accessible for most cytology laboratories","palabras_clave":"Tzanck|Herpes virus|Inmunofluorescencia directa|citologia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6423,"idioma_id":4,"titulo":"Activation of Epidermal Growth Factor Receptor (pEGFR) and Expression of Argentophilic Nucleolus Organizer Regions (AgNOR) in Seminomas","resumen":"Activation of epidermal growth factor receptor (p-EGFR) is one of the triggers in the developmentof many malignant tumors, and the measurement of Argentophilic Nucleolus Organizer Regions(AgNOR) area is used as a marker of tumor proliferation. Alpha actin smooth muscle (&#945;ASM) ofOriginal Research ArticleGuerra et al.; BJMMR, 13(9): 1-9, 2016; Article no.BJMMR.239332peritubular cells (PT) and Vimentin expression are modified during the expansion of the CIS\/intratubular germ cell neoplasia (ITGCN) to solid seminoma. We evaluated the expressionpEGFR, the AgNOR areas, the expression of &#945;ASM in PT and the Vimentin in neoplastic cells fromCIS\/ ITGCN to solid seminoma.28 formalin-fixed paraffin-embedded archival tissue blocks of Seminomas from the Departments ofPathology of Clinical Hospital and Ramos Mejía Hospital (CABA, Argentina) were used in thisstudy. pEGFR was expressed in CIS\/ ITGCN with membranous pattern (8\/9), switching to acytoplasmic pattern in the solid seminoma (15\/19). AgNOR areas of atypical gonocytes wereincreasing from CIS (3.5±0.3 µ2) to intratubular seminoma (3.8±0.4µ2) (p<.5), until solid seminoma(5.3±0.7 µ2) (p<.01). Increase of AgNOR areas is proportional to the expression of pEGFR.Conclusions: pEGFR was expressed in CIS\/ ITGCN with membranous pattern, switching to acytoplasmic pattern in the solid seminoma. The activation of this receptor could be the first step inthe mitogenic signal transduction.AgNOR areas complement diagnosis of testicular CIS, but not differentiate between CIS andintratubular seminoma. The value of AgNOR areas is maximum in the invasive seminoma, andstatistically different from intratubular seminoma. The intensity of &#945;ASM in PT decreased as thetumor progressed and Vimentin was negative in neoplastic cells.","palabras_clave":"seminoma|AgNOR|vimentin|recptor"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6424,"idioma_id":5,"titulo":"Facilitación de procesos colectivos para el reaprendizaje de la afectividad. Estudio de un Caso.","resumen":"Este artículo resulta de un proceso de investigación-acción implementado en una organización educativa de la Provincia de Santa Fe, es parte de una tesis doctoral que se inserta en las investigaciones desarrolladas en el Área de Aprendizaje y Desarrollo Organizacional de IRICE-Conicet. Asimismo se enmarca en un proyecto realizado mediante el de Programa de Apoyo a Proyectos de Investigación del sistema de ciencia, tecnología e innovación en la provincia de Santa Fe. Se profundiza aquí en el estudio de metodologías de facilitación de procesos colectivos para el reaprendizaje de la afectividad, que impliquen nuevos modos de abordaje y resolución respecto de la violencia. Las metodologías indagadas constituyeron perturbaciones eficaces para que la organización educativa estudiada iniciara un camino de transformación hacia la convivencia, superando las disociaciones presentes en la trama colectiva.","palabras_clave":"Facilitación|Reaprendizaje|afectividad|organizaciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6425,"idioma_id":4,"titulo":"Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus","resumen":"Infectious bronchitis virus (Gammacoronavirus, Coronaviridae) is a genetically variable RNA virus (27.6&nbsp;kb) that causes one of the most persistent respiratory disease in poultry. The virus is classified in genotypes with different epidemiological relevance and clinical implications. The present study reports the development and validation of specific RT-qPCR assays for the detection of two major IBV genotypes: South America I (SAI) and Asia\/South America II (A\/SAII). The SAI genotype is an exclusive and widespread South American lineage while the A\/SAII genotype is distributed in Asia, Europe and South America. Both identification assays employ TaqMan probes that hybridize with unique sequences in the spike glycoprotein gene. The assays successfully detected all the assessed strains belonging to both genotypes, showing high specificity and absence of cross-reactivity. Using serial dilutions of in vitro-transcribed RNA we obtained acceptable determination coefficients, PCR efficiencies and relatively small intra- and inter-assay variability. The assays demonstrated a wide dynamic range between 101?107 and 102?107 RNA copies\/reaction for SAI and A\/SAII strains, respectively. The possibility to characterize a large number of samples in a rapid, sensitive and reproducible way makes these techniques suitable tools for routine testing, IBV control, and epidemiological research in poultry.","palabras_clave":"Infectious Bronchitis|IBV|RT-qPCR|Genotype"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6426,"idioma_id":4,"titulo":"Postnatal   development and histofunctional differentiation of the oviduct in the  broad-snouted caiman (Caiman latirostris).","resumen":"Caiman latirostris is a South American crocodilian species characterized as a sentinel of the presence ofendocrine-disrupting compounds (EDCs). Evaluating developmental events in hormone-dependentorgans, such as the oviduct, is crucial to understand physiological postnatal development, to identifyputative periods of exposure sensitive to EDCs, and\/or to identify biomarkers useful to evaluate theeffects of EDC exposure. In this study, we describe the histomorphological features of C. latirostris oviductsby establishing the ontogeny of changes at cellular, tissue and molecular levels from the neonatalto the pre-pubertal juvenile stages. Since the histological diagnosis of the adenogenic oviduct lies on agroup of features, here we defined a histofunctional score system and a cut-off value to distinguishbetween preadenogenic and adenogenic oviducts. Our results showed that the maturation of theC. latirostris oviduct is completed postnatally and characterized by changes that mimic the pattern ofhistological modifications described for the mammalian uterus. Ontogenic changes in the oviductalepithelium parallel changes at subepithelial level, and include collagen remodeling and characteristicspatial-temporal patterns of a-actin and desmin. The expression pattern of estrogen receptor alphaand progesterone receptor evidenced that, even at early postnatal developmental stages, the oviduct ofC. latirostris is a target organ of endogenous and environmental hormones. Besides, oviductal adenogenesisseems to be an estrogen-dependent process. Results presented here provide not only insights into thehistophysiological aspect of caiman female reproductive ducts but also new tools to better characterizecaimans as sentinels of endocrine disruption.","palabras_clave":"ADENOGENESIS|COLLAGEN REMODELING|OVIDUCT|REPTILE|STEROID HORMONE RECEPTOR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6427,"idioma_id":4,"titulo":"Particulate matter pollution from a small coke-burning factory: soil magnetic screening and its relation with a simple atmospheric dispersion model","resumen":"Coal combustion processes lead to release of gases and particulate matter (PM) into the atmosphere that are often harmful to human health. These airborne pollutants seem to be dispersed and deposited in soils mainly according to the prevailing atmospheric conditions. Several trace elements can be found attached to PM as well as Fe-rich magnetic particles that can produce magnetic enhancement in the uppermost soil horizons. In the present work, we use a simple Gaussian Dispersion Model (GDM) for modelling the distribution of fine PM emission coming from a small coal (coke) burning factory in order to evaluate the relationship between such modelled data (PM distribution) and measured data (soil magnetic properties and trace metal contents). Our results show a strong spatial variation of concentration-dependent magnetic parameters based on a uniform magnetomineralogy in the overall study area. In addition, these results were analysed using multivariate statistics for 13 magnetic and chemical variables and the GDM results for two different atmospheric stability classes, and hence the in-situ magnetic susceptibility, anhysteretic and saturation remanent magnetization showed positive and statistically significant correlation with the GDM results (R = 0.70). Therefore, these results demonstrate the usefulness of magnetic properties in monitoring the PM distribution in soils or other environmental PM collectors.","palabras_clave":"magnetic parameters|heavy metals|Gaussian dispersion model|multivariate statistics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6428,"idioma_id":4,"titulo":"Magnetic properties of air suspended particles in thirty eight cities from south India","resumen":"Air pollution is a basic problem nowadays and it requires special concern. In India, the air pollution is a growing problem because of the enhanced anthropogenic activities such as burning fossil fuels involving industrial processes and motor vehicles. We study airborne dust particles collected at the height of 7 m in roadside and land area from thirty-eight cities in the state of Tamil Nadu. The collection involves a total of 111 samples concerning vehicular, industrial and residential areas, and allows us to assess the spatial distribution of magnetic particles produced and emitted on a short period of time (about one month). Magnetic properties of these air suspended particles were determined by techniques of environmental magnetism, revealing the presence of magnetite and hematite. We found the overall average of mass-specific magnetic susceptibility &#967; of 589.0 × 10&#8722;8 m3 kg&#8722;1 and saturation of remanent magnetization SIRM of 68.1 × 10&#8722;3 A m2 kg&#8722;1; as well as &#967; and SIRM values higher than 900.0 × 10&#8722;8 m3 kg&#8722;1 and 700.0 × 10&#8722;3 A m2 kg&#8722;1, respectively, corresponding to the most impacted zones in industrial\/vehicular areas and in cities located in the central\/eastern region respectively (e.g.: Hosur, Krishnagiri, Salem, Dharapuram, Ranipet, Ayanavaram, Cuddalore and Chidambaram). We analyzed the relationship between magnetic parameters, between areas and possible grouping of cities using multivariate statistical analysis. The SEM-EDS observations and grain size estimations reveal the presence of trace elements (Sb, Zn, Co, Ni, As and V) and fine particles (1?5 &#956;m) that can be inhaled and therefore are dangerous to human health.","palabras_clave":"MAGNETIC PARAMETERS|MAGNETITE|MULTIVARIATE STATISTICAL ANALYSIS|POLLUTION|SUSPENDED PARTICLE MATTER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6430,"idioma_id":5,"titulo":"?IMAGINARIOS TURÍSTICOS: ARGENTINA PARA EL TURISTA BRASILEÑO","resumen":"Las características del turismo internacional de la Argentina muestran que existe un importante flujo turístico desde Brasil, quien emerge como principal mercado emisor con una serie de valores e historia compartida y cierta cercanía sociocultural. Este trabajo tiene como objetivo estudiar el comportamiento del mercado brasileño, haciendo énfasis en cuáles son los principales atractivos visitados en Argentina. En primer lugar se analizan las principales propuestas teóricas vinculadas al imaginario, imagen y discursos en el turismo para comprender dicho fenómeno turístico. En segundo lugar, se estudia el rol de los principales actores intervinientes: el gobierno argentino, los intermediarios privados y el turista brasileño. Se observa que la configuración de dichos imaginarios turísticos privilegia ciertos destinos y productos turísticos respecto de otros y que a  pesar de los esfuerzos de promoción turística del sector público y privado para potenciar otros, dichas nuevas imágenes turísticas de la Argentina tardan en permear el imaginario de los brasileños.","palabras_clave":"PRODUCTO TURISTICO|IMAGINARIO TURISTICO|IMAGEN Y DISCURSOS EN TURISMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6431,"idioma_id":4,"titulo":"First Description of mcr-1 -Mediated Colistin Resistance in Human Infections Caused by Escherichia coli in Latin America","resumen":"Sin abstract.","palabras_clave":"mcr-1|Colistin|Escherichia coli|gen"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6433,"idioma_id":5,"titulo":"Desarrollo de películas a partir de nanocompuestos polipropileno\/talco para liberación de aromas","resumen":"Se estudia la liberación de aceite esencial de limón (aroma modelo) en películas sopladas de nanocompuestos a base de polipropileno (PP) conteniendo 5% p\/p de partículas de talco. En tal sentido, se utilizaron tres técnicas independientes y complementarias para estudiar dicho fenómeno físico, tales como: gravimetría, termogravimetría y espectroscopía infrarroja con transformada de Fourier. Los resultados obtenidos demostraron que las películas de nanocompuestos liberaron el aceite esencial de manera más lenta y prolongada en comparación con las del PP. Las partículas de talco actuaron como 'retén' del aceite esencial por su carácter absorbente. Además, obstaculizaron el pasaje de las moléculas aromáticas a través de la película por la disposición espacial que alcanzan en la matriz polimérica y por el incremento del grado de cristalinidad que inducen en el PP, debido al carácter nucleante de estas partículas.","palabras_clave":"poliolefinas|rellenos minerales|aceite de limón|difusión"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6434,"idioma_id":4,"titulo":"Collateral Reports and Cross-Informant Agreement about Adult Psychopathology in 14 Societies","resumen":"To advance international mental health assessment, instruments that have been internationally validated are needed. To this end, we analyzed ratings from 14 societies on the Adult Behavior Checklist (ABCL), a collateral-report form parallel to the Adult Self-Report (ASR; Achenbach and Rescorla 2003) for ages 18 to 59. Both the ABCL and the ASR assess problems, personal strengths, and adaptive functioning. For a sample of 8322 see note below collaterals, we found strong consistency across societies regarding which ABCL problem items tended to obtain relatively low, medium, or high ratings. Most societal effect sizes (ESs) for problem scale scores were small to medium (N = 8,302), we analyzed cross-informant agreement between self-reports on the ASR and collateral reports on the ABCL. Cross-informant correlations for problem scale scores averaged .47, with considerable societal variation. Problem score means were higher on the ASR than the ABCL in every society, but the size of the difference varied across societies. Mean item ratings on the ABCL and ASR were highly correlated within every society (mean r = .92), but within-dyad item rating agreement varied widely in every society (mean r = .39). In all societies, non-corroboration of self-reported deviance and of collateral-reported deviance was common. Overall findings indicated considerable similarity but also some important differences in collateral-reported problems and adaptive functioning across 14 societies.","palabras_clave":"Adult psychopathology|ABCL|ASR|Collateral-reported problems"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6435,"idioma_id":5,"titulo":"Peligrosidad y encierro penal","resumen":"Las medidas de seguridad constituyen la consecuencia jurídica prevista por la ley punitiva, distinta de la pena, aplicable a personas declaradas inimputables y a aquellas declaradas imputables que requieren algún tipo de prevención especial. Según su especificidad y tipo de finalidad a la que responden, estas intervenciones se encuentran clasificadas en nuestra legislación en tres grupos: medidas educativas, medidas de mejoramiento, y medidas curativas. Estas últimas en particular suponen instancias terapéuticas para el tratamiento de las personas que hubieran transgredido la ley condicionadas por algún tipo de padecimiento mental. Por ello se destinan a las personas declaradas inimputables (artículo 34, inciso 1 del Código Penal de la Nación) y a aquellas condenadas que dependan física o psíquicamente de algún tipo de estupefaciente (ley 23.737, artículos 16 y 17). A diferencia del sistema de penas, el sistema de medidas de seguridad encuentra su razón de ser en un argumento muy distinto al de la responsabilidad: el reproche social de la peligrosidad, donde la persona es considerada por la potencialidad de su comportamiento, al nivel de sus virtualidades y no de sus actos materiales.Así, la utilización de la peligrosidad como argumento jurídico-penal no sólo implica prácticas de identificación y marcación que contradicen el principio del derecho penal de acto que rige nuestro sistema jurídico, sino que legitima la institución y el sostenimiento del encierro de todo un sector de la población que incluye rutinas de vigilancia y corrección en el marco de prácticas de privación de la libertad en unidades psiquiátrico-penales. Todo bajo un único fundamento: la posibilidad de que la persona se dañe a sí misma, a terceros o a cualquier bien jurídicamente protegido.El artículo se propone entonces reflexionar sobre el concepto de peligrosidad que orienta en particular los discursos y las prácticas jurídico-penales en nuestro país, el cual tiene su origen en las ideas propuestas por la criminología positivista italiana de fines del siglo XIX. Son estos discursos, de hecho, los que inspiraron los actuales textos del  Código Civil, el Código Procesal Civil y Comercial de la Nación y el Código Penal de la Nación en materia de intervenciones sobre las personas con padecimiento mental, sus internaciones y tratamientos psicofarmacológicos. Es recién con la promulgación de la ley 26.657 de salud mental (fines de 2010) en el ámbito de la justicia civil y con los debates en torno a la reforma del código penal (iniciados en 2012), que las discusiones sobre la peligrosidad generaron algunos aportes novedosos, aunque como se desarrolla a lo largo del artículo, los resultados en la práctica resulten aún anclados en el mismo principio.","palabras_clave":"PELIGROSIDAD|ENCIERRO|CONTEXTOS JURÍDICO-PENALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6436,"idioma_id":4,"titulo":"Problems and Adaptive Functioning Reported by Adults in 17 Societies","resumen":"This study tested for similarities and differences across societies in self-ratings ofproblems, personal strengths, and aspects of adaptive functioning on the Adult Self-Report (ASR) for nonclinical samples of adults ages 18 to 59 in 17 societies (N 10,197). Results indicated considerable consistency across societies regarding meanratings on the ASR problem items. Most effect sizes (ESs) for societal differences inproblem scales were small (2?5%). Hierarchical linear modeling (HLM) analysesindicated that culture clusters and society accounted for small percentages of variancein Internalizing, Externalizing, and Total Problems scores, with most of the variationaccounted for by individual differences within societies. In contrast to the small effectsof society on problem scores, for the ASR Personal Strengths scale the societal ES was 34%and culture cluster accounted for 12% of the variance. Worse reported relations withspouse\/partner were associated with higher problem scores. Overall, findings indicatedconsiderable similarity but also some important differences in self-reported problems andadaptive functioning across 17 societies.","palabras_clave":"adult psychopathology|ASR|self-reported problems|cross-cultural"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6437,"idioma_id":4,"titulo":"Corporate Credit Spreads and the Sovereign Ceiling in Latin America","resumen":"We exploited a panel of 72 U.S. dollar-denominated bonds issued by Latin American publicly listed firms between 1996 and 2004, a period of regional financial crises, to answer the following three questions: (1) Is sovereign risk a statistically and economically significant determinant of the corporate credit spread, controlling for firm- and bond-specific characteristics? (2) If yes, do market participants apply the sovereign ceiling rule adopted by rating agencies in the pricing of our bond market data? (3) How do market views compare with the rating agencies ceiling policy for each corporate bond? We found strong evidence of an economically and statistically significant effect of sovereign risk on corporate spreads across different panel econometric specifications and bonds. Moreover, markets do not apply the ceiling rule in 77% to 90% of the bonds we sampled, and these findings are consistent with rating agencies? policies towards the latter for approximately 50% of the firms. These results are robust to the inclusion of firm- and bond-specific variables derived from the structural approach to credit risk and to the business cycle in each country.","palabras_clave":"Corporate  Bond Spreads|Latin America|Sovereign Ceiling|Credit Risk Models"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6439,"idioma_id":4,"titulo":"Vocal sac development and accelerated sexual maturity in the lesser swimming frog, Pseudis minuta (Anura, Hylidae)","resumen":"Sexual maturity involves the differentiation of the reproductive system, thematuration of germ cells, and the development of secondary sexual characteristics. Eventhough this topic has received much attention, little is known about the sequence ofevents that encompass reproductive maturation in anurans and what it could revealabout the developmental basis of life cycle evolution. The discovery of froglets ofPseudis minuta with incipient vocal sacs calling in breeding pools alongside severallarger adult specimens with fully-developed vocal sacs raised the question of the timingof sexual maturity in this species. Here we describe the sequence and timing ofdifferentiation, development and maturation of the vocal sac apparatus and the testes inPseudis minuta (Anura, Hylidae), in order to establish a timeline of events leading tosexual maturity. Differentiation of the vocal sac apparatus begins at the finalmetamorphic stages, earlier than reported for other species, and the vocal sac acquiresits final shape during the early postmetamorphic period. These modifications occur aftergonadal differentiation, which begins early during the larval period and proceeds with ahighly accelerated rate of development (e.g., secondary spermatids appear well beforemetamorphic climax), a situation reported previously for other anuran species only inthe genus Pseudis. These results, together with skeletochronological analysis showingthat some calling specimens presented no lines of arrested growth, indicate accelerationin the timing of sexual maturity in Pseudis, and raise questions about theinterdependence\/decoupling during development of the different components involvedin reaching the adult stage","palabras_clave":"anurans|gonadogenesis|juvenile period|life cycle|vocal sac"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6440,"idioma_id":4,"titulo":"Classical Characterization Techniques to Reveal the Structural Model of Nanocomposites with Bimetallic Monolayers of Porphyrins","resumen":"Nanocomposites with bimetallic monolayers of porphyrins were prepared. The wellordered metalloporphyrin monolayers covalently linked to the gold surface produce an important increase of the B band (&#8764;400 nm) shifted 20 nm relative to that of the related high-spin iron(III) complexes in solution. The position of the B band in the bimetallic architectures is highly dependent on the relative amount of the two porphyrins, showing the most significant shift for the SiO2\/APTES\/AuNp\/Fe-TPyP&M-TPyP (1:1) (30 nm, M = Ni(II) or Cu(II)). Resonance Raman based on the oxidation state marker bands (1553, 1354, and 390 cm&#8722;1) indicates that Fe-TPyP attached on gold nanoparticles adopts a low-spin Fe(II) conformation, which changes to Fe(II) intermediate spin or a low-spin Fe(III) in the presence of Cu-TPyP or Ni-TPyP. Surface-enhanced Raman scattering studies confirmed the hypothesis. MALDI-TOF analysis of the composites on gold nanoparticles was very useful in the detection of oxygenated forms of the metal complexes.","palabras_clave":"Hybrid nanocomposites|Nanoparticles|Raman spectroscopy| MALDI-TOF"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6441,"idioma_id":5,"titulo":"Federalismo electoral, fortaleza de las cuotas de ge&#769;nero y representacio&#769;n poli&#769;tica de las mujeres en Argentina y Me&#769;xico","resumen":"Este trabajo analiza el disen&#771;o de las cuotas de ge&#769;nero subnacionales en Argentina y Me&#769;xico y su relacio&#769;n con la representacio&#769;n descriptiva de las mujeres. Especi&#769;ficamente, se construye un i&#769;ndice que mide el nivel de fortaleza de la legislacio&#769;n de cuotas y se explora su asociacio&#769;n con las tasas de legisladoras electas tras las elecciones subnacionales de 2011-2013 en cada pai&#769;s. Los resultados muestran que la fortaleza de las cuotas incrementa la eleccio&#769;n de legisladoras provinciales en Argentina, mientras que las debilidades de la legislacio&#769;n en Me&#769;xico dotan de mayor margen de maniobra a los partidos e incrementan el peso del comportamiento estrate&#769;gico de las elites en la seleccio&#769;n de candidatas a los Congresos estatales.","palabras_clave":"Federalismo electoral|Leyes de cuota|Argentina|México"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6442,"idioma_id":5,"titulo":"La dimensión temporal del consumo de drogas  Análisis sociológico desde una categoría clave para el estudio de los procesos de salud-enfermedad-cuido","resumen":"Se analizan las dimensiones temporales que modulan los sentidos y las prácticas de varones y mujeres poli-consumidores de sustancias psicoactivas de 18 a 35 años residentes en el área metropolitana de Buenos Aires. La metodología se basó en el enfoque cualitativo; se realizaron 29 entrevistas en profundidad individuales, analizadas mediante un proceso de comparación constante de análisis entre las categorías generadas a partir de los datos y conceptos teóricos. Del análisis emergen prácticas y sentidos que regulan las diversas temporalidades que atraviesan el consumo de drogas, como son sentimientos vinculados a los ritmos corporales, las frecuencias entre consumo y consumo, las fases del ciclo de vida en las que se va a contratiempo, o las temporalidades inespecíficas que vuelven a un 'el momento' en el adecuado para el consumo. Prácticas que requieren una especial atención sobre el tiempo para denotar la flexibilidad que les permita consumir sin ser consumistas, drogarse sin ser adicto.","palabras_clave":"CONSUMIDORES DE DROGAS|TIEMPO|SOCIOLOGÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6443,"idioma_id":5,"titulo":"Herederos de Beijing: las trayectorias del mainstreaming de género en Montevideo y Rosario","resumen":"Este artículo contribuye al debate sobre Beijing + 20 a partir de un análisisdel mainstreaming de género en dos ciudades que se consideraron casosejemplares: Montevideo (capital de Uruguay) y Rosario (Argentina).En particular, se busca responder la siguiente pregunta:¿cuánto avanzóefectivamente el mainstreaming en Montevideo y en Rosario, tomandoen cuenta los estándares de la Plataforma de Beijing? En vistas de queambos casos tienen políticas explícitas de mainstreaming, el análisis empíricose centra en el progreso de su implementación efectiva. Como semuestra, se trata de políticas que avanzaron con diferente intensidad enlas dos dimensiones del mainstreaming destacadas por la Plataforma deBeijing: el empoderamiento de las mujeres y la consolidación de capacidadese instrumentos de gestión estatal.","palabras_clave":"Mainstreaming de género|Políticas de género|Montevideo|Rosario"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6444,"idioma_id":5,"titulo":"El derecho a la ciudad. Una mirada desde América Latina","resumen":"El objetivo de este artículo es indagar la perspectiva latinoamericana respecto del ?derecho a la ciudad?. Sostenemos que el predominio de una perspectiva crítica contribuye a configurar una mirada alternativa al pensamiento dominante. Para ello se profundiza en el análisis de dos tensiones. Por un lado, la existente entre el modelo de sociedad propuesto e implementado por el neoliberalismo frente al actualmente impulsado en ciertos países de la región y con distintos matices por el post-neoliberalismo. Por otro lado, la disputa respecto al significado atribuido al derecho a la ciudad.","palabras_clave":"Derecho a la ciudad|América Latina|perspectiva crítica|neoliberalismo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6445,"idioma_id":4,"titulo":"Grape pomace and grape pomace extract improves insulin signaling in high-fat-fructose fed rats-induced metabolic syndrome","resumen":"In this study the effect of diet supplementation with grape pomace (GP) and grape pomace extract (GPE) on insulin sensitive tissues (adipose, liver and muscle) was evaluated in an experimental model of metabolic syndrome (MetS). MetS was developed by giving a high-fat-fructose (HFF) diet to Wistar rats. Six weeks of HFF diet induced weight gain, which was partially attenuated by GP (1 g Kg-1 day-1) and GPE (300 mg Kg-1 day-1) supplementation. HFF diet increased systolic blood pressure, triglycerides, insulin resistance (HOMA:IR) and inflammation (c-reactive protein (CRP)). Supplementation with GP prevented SBP, triglycerides and CRP increased and partially attenuated insulin resistance. On the other hand, GPE partially reduced SBP and triglycerides and significantly prevented insulin resistance and inflammation. Also, HFF diet induced higher triglycerides content and enhanced NADPH oxidase activity in liver. As well, HFF diet increased epididymal adipose tissue weight, enlarged adipocytes size, and cjun N-terminal kinase (JNK) activation, probably contributing with a proinflammatory cytokine pattern (higher resistin) and lower adiponectin protein expression. These alterations may result in an impairment of insulin signaling cascade observed in adipose, liver and muscle tissue (IRS1, Akt, and extracellular signal-regulated kinases (ERK1\/2)) from HFF rats. Supplementation with GP and in a greater extent GPE attenuated liver triglyceride content and adiposity and restored adipose, liver and muscle response to insulin. These findings show that supplementation with GP and GPE in a greater extent can counteract adiposity, inflammation, liver damage and impaired insulin signaling associated to MetS, supporting the utilization of winemaking residues in food industry\/human health due to their high amount of bioactive compounds.","palabras_clave":"Adiposity|Grape pomace extract|Grape pomace|High-fat-fructose diet|Insulin signaling"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6446,"idioma_id":5,"titulo":"Comparación entre las normas de desempeño térmico edilício de Argentina y Brasil","resumen":"l objetivo de este estudio es realizar una revisión de la normativa de desempeño térmico destinada a edificios de viviendas, vigente en Argentina y Brasil. Se busca detectar las coincidencias y divergencias más significativas, así como la trayectoria y dificultades para su implementación, con la finalidad de proponer acciones y mejoras. Se asume que con un programa de gestión adecuado que incluya participación social y profesional, se podrá aumentar el nivel de compromiso ambiental, la aplicación efectiva de normas, como un requisito para modificar la cultura vigente en la edificación. El análisis se basó en el examen de documentos normativos y legales sobre el desempeño térmico edilicio, además del material libre disponible. De Argentina, se analizó el conjunto de las normas IRAM enumeradas en el Decreto 1.030\/10, que reglamenta la Ley 13.059\/03 y el código de edificaciones de Rosario. Para Brasil se estudió la NBR 15220\/08 y la 15575\/13. Se contrastaron las normas citadas y se encontró que en cuanto a valores exigidos en transmitancia térmica para muros, la IRAM es 2 veces más exigente que la NBR en verano y 3,5 más, en invierno. Finalmente se proponen recomendaciones que contribuyan al mejoramiento en la aplicación del desempeño térmico del sector residencial. ","palabras_clave":"Normativa|Brasil|Argentina|Desempeño térmico edilicio|Edificaciones residenciales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6448,"idioma_id":4,"titulo":"Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463","resumen":"Laccases are multicopper polyphenol oxidases that are able to catalyze the oxidation of a wide range of phenolic compounds with the simultaneous reduction of O2 to H2O. Despite their promising industrial uses, feasible incorporation of laccases in harsh processes requires the bioprospecting and\/or engineering of enzymes to be stable and active in acidic or alkaline pHs, high temperatures, oxidative conditions and tolerant to high salinity and\/or organic solvents. Here we used a PCR-based screening to clone two novel laccase coding sequences from the white-rot basidiomycete Trametes trogii. Recombinant expression of lcc3 gene in Komagataella (=Pichia) pastoris showed that it encodes a thermo active and thermostable laccase with an optimum temperature of 50°C and with a half-life of 45 minutes at 70°C and a stability higher than 3 h at 60°C. Furthermore, recombinant LCC3 was capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C.","palabras_clave":"Pichia pastoris|Decoloración de tintes|Lacasa termoestable|Trametes trogii"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6449,"idioma_id":5,"titulo":"Calidad del empleo en Argentina (2004-2011) Una crítica al enfoque de las credenciales educativas","resumen":"El trabajo postula que si bien las políticas desarrolladas en la actual fase de crecimiento han favorecido la ampliación de la demanda de empleo, este proceso no evidencia -al menos todavía- un cambio cualitativo en lo que refiere a una disminución de la heterogeneidad socio-productiva. En este sentido, se sostiene que continuaría vigente la segmentación de los mercados laborales y una alta concentración de recursos ?incluida la fuerza de trabajo con mejores credenciales educativas- sobre los sectores más dinámicos del sistema económico. La información utilizada surge de los microdatos de la Encuesta Permanente de Hogares del INDEC (EPH-Continua), y corresponden al total urbano relevado por dicha encuesta para los años testigo considerados en el estudio (2004, 2007 y 2011).","palabras_clave":"HETEROGENEIDAD ESTRUCTURAL|SEGMENTACIÓN DEL MERCADO LABORAL|CAPITAL EDUCATIVO|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6450,"idioma_id":4,"titulo":"Pseudoscalar top-Higgs coupling: Exploration of CP-odd observables to resolve the sign ambiguity","resumen":"We present a collection of mathrm{CP}-odd observablesfor the process pp o t (o b {ell}^+ u_{ell}) ar{t} (o ar{b} {ell}^-ar{u}) H that are linearly dependent on the scalar(kappa_t) and pseudoscalar (kappa_t) top-Higgs coupling and hencesensitive to the corresponding relative sign. The proposed observablesare based on triple product (TP) correlations that we extractfrom the expression for the differential cross section in terms of thespin vectors of the top and antitop quarks. In order to explore otherpossibilities, we progressively modify these TPs, firstby combining them, and then by replacing the spin vectors by thelepton momenta or the t and ar{t} momenta by their visible parts.We generate Monte Carlo data sets for several benchmark scenarios,including the Standard Model (kappa_t=1, ilde{kappa}_t=0) andtwo scenarios with mixed $mathrm{CP}$ properties (kappa_t=1,&nbsp; ilde{kappa}_t=pm 1).Assuming an integrated luminosity that is consistent with thatenvisioned for the High Luminosity Large Hadron Collider, using Monte Carlo-truth and taking into account only statistical&nbsp; uncertainties, we find that the most promising observable can disentangle the ``mathrm{CP}-mixed´´ scenarios with an effective separation of sim 19sigma. In the case of observables that do not require the reconstruction of the t and ar{t} momenta, the power of discrimination is up to sim13sigma for the same number of events. We also show that the most promising observables can still disentangle the mathrm{CP}-mixed scenarios when the number of events is reduced to values consistent with expectationsfor the Large Hadron Collider in the near term.","palabras_clave":"BEYOND STANDARD MODEL|CP VIOLATION|HIGGS PHYSICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6451,"idioma_id":4,"titulo":"Pichia   pastoris engineering for the production of a modified phospholipase C","resumen":"Crude vegetable oils are refined to remove impurities that adversely impact in their stability, color and flavor. In recent years, enzymatic degumming methods using phospholipase C (PLC) enzymes provide an environmentally friendly process for phospholipids removal with improved oil recovery yields.In this study, Pichia pastoris was used as the expression system for the production of PLC-Y, a modified PLC enzyme derived from Bacillus cereus. Production of secreted PLC-Y driven by the methanol inducible AOX1 promoter was optimized by genetic strain engineering which included gene codon optimization, generation of multi-copy chromosomal integrations and the co-expression of helper factors supporting protein folding, processing and secretion processes. In addition, tunable promoters directing helper factor expression were tested.In batch cultures, a strain harboring seven integrated copies of the PLC-Y expression cassette and co-expressing the HAC1 transcription factor under an attenuated AOX1 promoter showed a 6.2 fold increase in the production titers compared to the strain harboring a single gene copy. A fed-batch fermentation process developed using this engineered strain produced 4.5 g\/l of this enzyme. The results presented in this work show the viability of using PLC-Y for oil degumming and provide a manufacturing process for its cost effective production.","palabras_clave":"Oil degumming|Phospholipase C|Green chemistry|synthetic biology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6452,"idioma_id":4,"titulo":"Novel electrochemical properties of an emergent mycotoxin: Sterigmatocystin","resumen":"The electrochemical reduction of sterigmatocystin in acetonitrile +0.1 M tetrabutylammonium perchlorate at glassy carbon electrodes is studied for the first time using cyclic and square wave voltammetries, and controlledpotential electrolysis. Two reduction peaks centered at&#8722;1.77 and &#8722;2.33 V vs. Ag\/AgCl were found, which correspond to the formation of the radical anion and dianion, respectively. The overall electrode process was diffusion controlled. The initially formed radical anion undergoes an irreversible dimerization reaction with a rate constant of 2.06 × 104M&#8722;1 s&#8722;1, producing a basic dimeric dianion, which is protonated by the startingmolecule. An unstable dimeric reaction productwas detected by UHPLC-MS\/MSmeasurements. A value of (3.1±0.9) ×10&#8722;5 cm2 s&#8722;1 was calculated for sterigmatocystin diffusion coefficient fromconvoluted cyclic voltammograms. Thermodynamic and kinetics parameters were determined from digital simulation of cyclic voltammograms. Probable chemical structures for the dimer are proposed based on the results of theoretical calculations. The effect of the addition of tetrabutylammonium hydroxide, trifluoroacetic acid and water on the voltammetric signals was also investigated.The quantitative determination of sterigmatocystin was carried out by square wave voltammetry using the commercial reagent. The calibration curve was linear in the concentration range from 0.050 to 11.2 ppm, and the limits of detection and quantification were 10 and 33 ppb for signal to noise ratios of 3:1 and 10:1, respectively.","palabras_clave":"Micotoxina|Esterigmatocistina|Reducción electroquímica|Voltamperometría Cíclica|Voltamperometría de onda cuadrada|Simulación digital"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6453,"idioma_id":4,"titulo":"Handling time misalignment and rank deficiency in liquid chromatography by multivariate curve resolution: quantitation of five biogenic amines in fish","resumen":"Biogenicamines(BAs)areusedforidentifyingspoilageinfood.Themostcommonaretryptamine(TRY),2-phenylethylamine(PHE),putrescine(PUT),cadaverine(CAD)andhistamine(HIS).Duetolackofchro-mophores,chemicalderivatizationwithdansylwasemployedtoanalyzetheseBAsusinghighperfor-manceliquidchromatographywithadiodearraydetector(HPLC-DAD).However,thederivatizationre-actionoccurswithanyprimaryorsecondaryamine,leadingtoco-elutionofanalytesandinterferentswithidenticalspectralprofiles,andthuscausingrankdeficiency.Whenthespectralprofileisthesameandpeakmisalignmentispresentonthechromatographicruns,itisnotpossibletohandlethedataonlywithMultivariateCurveResolutionandAlternativeLeastSquare(MCR-ALS),byaugmentingthetime,orthespectralmode.Awaytocircumventthisdrawbackistoreceiveinformationfromanotherde-tectorthatleadstoaselectiveprofilefortheanalyte.Toovercomebothproblems,(tri-linearitybreakintime,andspectralmode),thispaperproposesanewanalyticalmethodologyforfastquantitationoftheseBAsinfishwithHPLC-DADbyusingtheicoshiftalgorithmfortemporalmisalignmentcorrectionbeforeMCR-ALSspectralmodeaugmentedtreatment.Limitsofdetection,relativeerrorsofprediction(REP)andaveragerecoveries,rangingfrom0.14to0.50&#956;gmL&#8722;1,3.5?8.8%and88.08%?99.68%,respec-tively.Theseareoutstandingresultsobtained,reachingquantificationlimitsforthefiveBAsmuchlowerthanthoseestablishedbytheFoodandAgricultureOrganizationoftheUnitedNationsandWorldHealth","palabras_clave":"Biogenic amines|Dansyl derivatization|Rank deficiency|High performance liquid chromatography|Icoshift|Multivariate Curve Resolution"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6454,"idioma_id":5,"titulo":"Tribulaciones a la hora de traducir poesía. ?La mentira?, de Ósip Mandelshtam","resumen":"Traducir poesía exige una tarea queatienda a la multiplicidad de planos que construyen el poema. En principio, espreciso sustraerse a la dicotomía (famosa en Occidente) entre forma ycontenido. La traducción literariaoccidental suele privilegiar el contenido por sobre la forma. En todo caso, laforma es lo primero que el traductor parece dispuesto a resignar.El trabajo propone la deconstrucción analítica de lapoesía ?La mentira?, de Ósip Mandelshtam, y la tentativa de su reconstrucciónen castellano tratando de mantener los aspectos diversos del original:el métrico, el sintáctico, el semántico, elestilístico. La poesía rusa en general essílabo-tónica, se construye sobre metros clásicos regulares. Los anapestos delpoema en cuestión son tan relevantes como el aspecto temático. El estilocoloquial, familiar del lenguaje, acorde con la ambientación campesina que seofrece, es otro rasgo fundamental a preservar. La lengua castellana es capaz deacoger todos estos planos, aunque presente sus resistencias. Con todo, elriesgo es que la poesía quede del otrolado.","palabras_clave":"LITERATURA RUSA|ACMEÍSMO|MANDELSHTAM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6455,"idioma_id":4,"titulo":"The anthraquinones rubiadin and its 1-methyl ether isolated from Heterophyllaea pustulata reduces Candida tropicalis biofilms formation","resumen":"Background: Candida tropicalis is increasingly becoming among the most commonly isolated pathogens causing fungal infections with an important biofilm-forming capacity. Purpose: This study addresses the antifungal effect of rubiadin (AQ1) and rubiadin 1-methyl ether (AQ2), two photosensitizing anthraquinones (AQs) isolated from Heterophyllaea pustulata , against C. tropicalis biofilms, by studying the cellular stress and antioxidant response in two experimental conditions: dark- ness and irradiation. The combination with Amphotericin B (AmB) was assayed to evaluate the synergic effect. Study design\/Methods: Biofilms of clinical isolates and reference strain of Candida tropicalis were treated with AQs (AQ1 or AQ2) and\/or AmB, and the biofilms depletion was studied by crystal violet and confocal scanning laser microscopy (CSLM). The oxidant metabolites production and the response of antioxidant defense system were also evaluated under dark and irradiation conditions, being the light a trigger for photo-activation of the AQs. The Reactive Oxygen Species (ROS) were detected by the reduction of Nitro Blue Tetrazolium test, and Reactive Nitrogen Intermediates (RNI) by the Griess assay. ROS accumulation was also detected inside biofilms by using 2  ,7  -dichlorodihydrofluorescein diacetate (DCFH-DA) probe, which was visualized by CSLM. Superoxide dismutase (SOD) activity and the total antioxidant capacity of biofilms were measured by spectrophotometric methods. The minimun inhibitory concentration for sessile cells (SMIC) was determined for each AQs and AmB. The fractional inhibitory concentration index (FICI) was calculated for the combinations of each AQ with AmB by the checkerboard microdilution method. Results: Biofilm reduction of both strains was more effective with AQ1 than with AQ2. The antifungal effect was mediated by an oxidative and nitrosative stress under irradiation, with a significant accumu- lation of endogenous ROS detected by CSLM and an increase in the SOD activity. Thus, the prooxidant- antioxidant balance was altered especially by AQ1. The best synergic combination with AmB was also obtained with AQ1 (80.5%) (FICI = 0.74). Conclusion: Under irradiation, the oxidative stress was the predominant effect, altering the prooxidant- antioxidant balance, which may be the cause of the irreversible cell injury in the biofilm. Our results showed synergism of these natural AQs with AmB. Therefore, the photosensitizing AQ1 could be an alter- native for the Candida infections treatment, which deserves further investigation.","palabras_clave":"Biofilms|Antifungal| Oxidative stress|synergistic activity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6456,"idioma_id":4,"titulo":"Selection and characterization of Argentine isolates of Trichoderma harzianum for effective biocontrol of Septoria leaf blotch of wheat","resumen":"Abstract Species of the genus Trichoderma are economicallyimportant as biocontrol agents, serving as a potentialalternative to chemical control. The applicability of Trichodermaisolates to different ecozones will depend on thebehavior of the strains selected from each zone. The presentstudy was undertaken to isolate biocontrol populations ofTrichoderma spp. from the Argentine wheat regions and toselect and characterize the best strains of Trichoderma harzianumby means of molecular techniques. A total of 84 outof the 240 strains of Trichoderma were able to reduce thedisease severity of the leaf blotch of wheat. Thirty-sevenstrains were selected for the reduction equal to or greater than50 % of the severity, compared with the control. The percentagevalues of reduction of the pycnidial coverage rangedbetween 45 and 80 %. The same last strains were con&#64257;rmedas T. harzianum by polymerase chain reaction ampli&#64257;cationof internal transcribed spacers, followed by sequencing.Inter-simple sequence repeat was used to examine thegenetic variability among isolates. This resulted in a total of 132 bands. Further numerical analysis revealed 19 haplotypes,grouped in three clusters (I, II, III). Shared strains, withdifferent geographical origins and isolated in different years,were observed within each cluster. The origin of the isolatesand the genetic group were partially related. All isolates fromParana´were in cluster I, all isolates from Lober&#305;´a were incluster II, and all isolates from Pergamino and Santa Fe werein cluster III. Our results suggest that the 37 native strains ofT. harzianum are important in biocontrol programs and couldbe advantageous for the preparation of biopesticides adaptedto the agroecological conditions of wheat culture.","palabras_clave":"BIOLOGICAL CONTROL|TRICHODERMA HARZIANUM|SEPTORIA|ISSR"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6457,"idioma_id":4,"titulo":"Empirical demonstration of hybrid chromosomal races in house mice","resumen":"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:'Times New Roman';mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Cambria;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:'Times New Roman';mso-bidi-theme-font:minor-bidi;}@page Section1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;}--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;Western house mice&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:'Times New Roman';mso-ascii-font-family:Cambria;mso-fareast-font-family:Cambria;mso-hansi-font-family:Cambria;mso-bidi-font-family:'Times New Roman';}@page Section1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;}--&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;(Mus musculus domesticus)and common shrews (Sorex araneus) are importantmodels for study of chromosomal speciation. Both had ancestral karyotypesconsisting of telocentric chromosomes, and each is subdivided into numerouschromosomal races many of which have resulted from fixation of new mutations(Robertsonian fusions and whole-arm reciprocal translocations). However, somechromosomal races in both species may alternatively have originated throughhybridization, with particular homozygous recombinant products reachingfixation. Here, we demonstrate the process of generation of hybrid chromosomalraces for the first time in either species using molecular markers. Analysis ofcentromeric microsatellite markers show that the Mid Valtellina (IMVA) andUpper Valtellina (IUVA) chromosomal races of the house mouse are recombinantproducts of hybridization of the Lower Valtellina (ILVA) and Poschiavo (CHPO)chromosomal races, supporting earlier theoretical analysis. IMVA and IUVAoccupy a small area of the Italian Alps where ILVA makes contact with CHPO.IUVA and CHPO have previously been shown to be reproductively isolated in onevillage, emphasizing that hybrid chromosomal races in small mammals, as inplants, have the potential to be part of the speciation process.","palabras_clave":"CHROMOSOMAL SPECIATION|HYBRIDIZATION|MUS MUSCULUS DOMESTICUS|ROBERTSONIAN FUSION|RECOMBINATIONAL SPECIATION|ZONAL RACIATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6458,"idioma_id":4,"titulo":"Decrease of a current mediated by Kv1.3 channels causes striatal cholinergic interneuron hyperexcitability in experimental parkinsonism","resumen":"The mechanism underlying a hypercholinergic state in Parkinson?s disease (PD) remains uncertain. Here, we show that disruption of the Kv1 channel-mediated function causes hyperexcitability of striatal cholinergic interneurons in a mouse model of PD. Specifically, our data reveal that Kv1 channels containing Kv1.3 subunits contribute significantly to the orphan potassium current known as IsAHP in striatal cholinergic interneurons. Typically, this Kv1 current provides negative feedback to depolarization that limits burst firing and slows the tonic activity of cholinergic interneurons. However, such inhibitory control of cholinergic interneuron excitability by Kv1.3-mediated current is markedly diminished in the parkinsonian striatum, suggesting that targeting Kv1.3 subunits and their regulatory pathways may have therapeutic potential in PD therapy. These studies reveal unexpected roles of Kv1.3 subunit-containing channels in the regulation of firing patterns of striatal cholinergic interneurons, which were thought to be largely dependent on KCa channels.","palabras_clave":"Kv1.3 channels|Parkinson?s disease|Striatal Cholinergic Interneuron"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6459,"idioma_id":4,"titulo":"Hypothyroidism advances mammary involution in lactating rats through inhibition of PRL signaling and induction of LIF\/STAT3 mRNAs","resumen":"Thyroid diseases have deleterious effects on lactation, litter growth and survival, and hinder the suckling-induced hormone release, leading in the case of hyperthyroidism, to premature mammary involution. To determine the effects of hypothyroidism (HypoT) on late lactation, we analyzed the effect of chronic 6-propyl-2-thiouracil (PTU)-induced HypoT on mammary histology and the expression of members of the JAK\/STAT\/SOCS signaling pathway, milk proteins, prolactin (PRLR), estrogen (ER), progesterone (PR) and thyroid hormone (TR) receptors, markers of involution (such as stat3, lif, bcl2, BAX and PARP) on lactation (L) day 21. HypoT mothers showed increased histological markers of involution compared with control rats, such as adipose\/epithelial ratio, inactive alveoli, picnotic nuclei and numerous detached apoptotic cells within the alveolar lumina. We also found decreased PRLR, &#946;-casein and &#945;-lactoalbumin mRNAs, but increased SOCS1, SOCS3, STAT3 and LIF mRNAs, suggesting a decrease in PRL signaling and induction of involution markers. Furthermore, Caspase-3 and 8 and PARP labeled cells and the expression of structural proteins such as &#946;-Actin, &#945;-Tubulin and Lamin B were increased, indicating the activation of apoptotic pathways and tissue remodelation. HypoT also increased PRA (mRNA and protein) and er&#946; and decreased er&#945; mRNAs, and increased strongly TR&#945;1, TR&#946;1, PRA and ER&#945; protein levels. These results show that lactating HypoT rats have premature mammary involution, most probably induced by the inhibition of prolactin signaling along with the activation of the LIF-STAT3 pathway.","palabras_clave":"PRL SIGNALING PATHWAY|NUCLEAR RECEPTORS|STAT3|LIF|CASPASES|TISSUE REMODELING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6460,"idioma_id":4,"titulo":"Molecular analysis of methicillin-resistant Staphylococcus aureus dissemination among healthcare professionals and\/or HIV patients from a tertiary hospital","resumen":"Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a nosocomial pathogen in community settings. MRSA colonized individuals may contribute to its dissemination; the risk of MRSA infection is increased in human immunodefi ciency virus\/acquired immune defi ciency syndrome (HIV\/AIDS) patients, although the prevalence of colonization in this group is not well established. The present study addressed this issue by characterizing MRSA isolates from HIV\/AIDS patients and their healthcare providers (HCPs) to determine whether transmission occurred between these two populations. Methods: A total of 24 MRSA isolates from HIV-infected patients and fi ve from HCPs were collected between August 2011 and May 2013. Susceptibility to currently available antimicrobials was determined. Epidemiological typing was carried out by pulsed-fi eld gel electrophoresis, multilocus sequence typing, and Staphylococcus cassette chromosome (SCCmec) typing. The presence of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and heterogeneous daptomycin-resistant Staphylococcus aureus (hDRSA) was confi rmed by population analysis profi le. Isolates characterized in this study were also compared to isolates from 2009 obtained from patients at the same hospital. Results: A variety of lineages were found among patients, including ST5-SCCmecII and ST30-SCCmecIV. Two isolates were Panton-Valentine leukocidin-positive, and hVISA and hDRSA were detected. MRSA isolates from two HCPs were not related to those from HIV\/AIDS patients, but clustered with archived MRSA from 2009 with no known relationship to the current study population. Conclusions: ST105-SCCmecII clonesthat colonized professionals in 2011 and 2012 were already circulating among patients in 2009, but there is no evidence that these clones spread to or between HIV\/AIDS patients up to the 7th day of their hospitalization","palabras_clave":"Methicilin-resistant Staphylococcus aureus|HIV patients|Teicoplanin resistance|h-VISA. Daptomycin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6461,"idioma_id":4,"titulo":"Utilization of fluorescence spectroscopy as a novel approach to evaluate the oxidative stability in beef retail displayed","resumen":"Beef samples from grazing steers finished with different seed-supplemented diets were vacuum packaged for 3,14 and 56 days (VC) and subsequently exposed to aerobic conditions (AE) for 0 and 5 days. Different fluorescent compounds of interest in the oxidation process were detected in meat, namely tryptophan residues, Schiff bases and porphyrins. Tryptophan intensity fluorescence increased with 14 days of VC; while Schiff bases intensity increased(P < 0.05) in beef samples stored under VC-56 and in all samples after AE-5 days. Porphyrins increased (P < 0.05) gradually with the extension of vacuum storage time, but were degraded in beef with long vacuum storage and 5 days of AE. Higher levels of porphyrins in beef under VCwere correlated (P b 0.05) with lower redness and higher TBARS after AE-5. This study revealed the potential of fluorescence signals to detect oxidative changes in beef under different storage conditions using a fast and nondestructive method such as fluorescencespectroscopy.","palabras_clave":"FLUORESCENT COMPOUNDS|MEAT|OXIDATION|SHELF LIFE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6463,"idioma_id":4,"titulo":"Methane emissions from sheep grazing Pearl millet (Penissetum americanum (L.) Leeke) swards fertilized with increasing nitrogen levels","resumen":"This study aimed at quantifying methane emissions from sheep grazing pearl millet swards (Penissetum americanum (L.) Leeke) as affected by nitrogen (N) fertilization doses (50, 100, 200 and 400 kg N ha&#8722;1). The experimental period was 70 days of pasture use, from February to April 2011. The grazing method was continuous stocking with variable stocking rate, so pasture structure was intended to be similar (30 cm sward height) among treatments. Thirty six tester animals aging 5 months and initially weighing 20 ± 1.6 kg were used for evaluations. Intake (OMI) was estimated by their relationship with N content on faeces. The sulfur hexafluoride (SF6) technique was used for two sampling periods of five consecutive days each to quantify daily methane (CH4) production. Parameters related to sward structure was not affected by N fertilization, but herbage accumulation increased linearly (P  0.05) OMI, and methane emissions expressed as a ratio of OMI. Results indicated that increasing nitrogen doses decrease methane emissions per animal. When expressed as grams of methane per kilogram of organic matter ingested or energy converted into methane, no difference was observed. However, emissions per unit area increase as a consequence of higher stocking rates allowed by the increase in forage production. Sheep emitted 16.3 g of methane per kg of ingested organic matter and converted 5.1% of ingested gross energy into methane.","palabras_clave":"GHG|SHEEP METHANE EMISSION|STOCKING RATE|TROPICAL PASTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6464,"idioma_id":4,"titulo":"Electric birefringence of aqueous solutions of a rigid polyelectrolyte. Polarization mechanisms and anomalous birefringence signals","resumen":"We present an electric birefringence study of aqueous solutions of an intrinsically rigid polyelectrolyte, xanthan, and of mixed solutions with an oppositely charged surfactant, DTAB. The stationary electric birefringence as well as built-up and decay processes give information on the polarization mechanisms of the polyelectrolyte and on the role played by counterions. By adding a cationic surfactant (DTAB), that replaces the small Na+  counterions of the polymer, we could obtain further insights on the role of condensed counterions in these mechanisms. In some cases, anomalous birefringence signals appear, revealing a new polarization mechanism likely due to field induced interactions within polymer-surfactant aggregates.","palabras_clave":"Electric birefringence|Polyelectrolytee"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6465,"idioma_id":4,"titulo":"The Pacific-Atlantic connection: biogeochemical signals in the southern end of the Argentine shelf","resumen":"The Cape Horn current transports low-salinity waters from the SE Pacific Ocean into the Atlantic, which are transported further north by the Malvinas current. Biogeochemical signals of this connection were studied by characterization of dissolved organic matter (DOM) by determination of dissolved organic carbon (DOC), fluorescent dissolved organic matter (FDOMc), and DOM humification index (HIX). Further, inorganic nutrients, salinity, temperature, stable isotopic composition of particulate organic nitrogen (&#948;15N) and chlorophyll a (Chla) were measured in the southern end of the Argentine shelf in March 2012. Three water types were characterized: waters of the Beagle Channel (BCW), coastal waters (CW) and oceanic waters (OW). Highest values of ammonium, DOC, FDOMc and HIX were found in BCW, the lowest in OW, suggesting that terrigenous input is a main source of ammonium and refractory carbon, which is supported by a highly significant inverse correlation of these parameters with salinity. In turn, lowest concentrations of nitrate, silicate and phosphate were found in BCW and CW, and highest in OW, with highly significant correlations of these nutrients with salinity, indicating the contribution of the saltier, nutrient-rich Antarctic Circumpolar Current (ACC) to the Pacific-Atlantic connection system. In general there was an inverse distribution pattern between Chla and those nutrients contributed by the ACC, which is consistent with the transition from coastal waters to the low-silicate, high-nitrate, low-chlorophyll, iron-limited setting of the Subantarctic oceanic waters north of the Polar Front. In contrast, in the low-salinity, internal BCW, high values of ammonium, DOC, HIX and FDOM indicate continental inputs, likely including iron complexes, which could have led to the observed high Chla values. &#948;15N values were positive in the study region, and same as ammonium, reached a maximum in the inner part of the BCW, declining towards OW. This does not support a previous assumption that rainfall on the SE Pacific could be the source of ammonium and hence explain negative &#948;15N values previously found in the northern Drake Passage. The highly significant inverse correlations of ammonium, FDOMc, HIX, and DOC with salinity suggest that continental runoff rather than wet deposition is an important source of ammonium and DOM in the Pacific-Atlantic connection.","palabras_clave":"PACIFIC-ATLANTIC |CONNECTION|NUTRIENTS-ORGANIC-MATTER|ISOTOPES|TERRIGENOUS|INPUT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6466,"idioma_id":4,"titulo":"Detection of glaciers displacement time-series using SAR","resumen":"Glaciers are sensitive indicators of climate change. Particularly, glacier surface velocity constitutes a key parameter for estimating ice volume variations as response to global warming and its incidence in sea level rise. Several methodologies based in remotely sensed data have been employed for estimating ice velocity fields. They are mostly based in cross-correlating pairs of images in order to track features displacement between two dates. High ice flux velocity, which can reach more than 1 km\/year, constitute a challenge for the existing methodologies, in practice limiting to a few days the time span between useful data. In this work we present an extension of the known Pixel Offset ? Small Baseline Subsets (PO-SBAS) technique, that profit a set of successive Synthetic Aperture Radar (SAR) scenes for computing displacement time series and ice velocity fields. The algorithm is guided by a preliminary ice velocity model estimated from the data itself, which significantly improves the results reliability and reduces the overall computational cost. Furthermore, it implements a processing scheme that considers the displacement estimations (PO) quality in order to decide which pixels are included in the timeseries inversion. The proposed technique is applied to 22 COSMO-Skymed SAR images of Viedma Glacier (Southern Patagonian Icefield, Argentina) spanning roughly a year. The results obtained are robust and make profit of the whole available dataset. Resulting mean velocity field and displacement time series show the algorithm suitability for retrieving and characterizing complex ice motion patterns.","palabras_clave":"SAR|GLACIAR VIEDMA|VELOCITY FIELDS|SOUTH PATAGONIA ICEFIELD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6467,"idioma_id":4,"titulo":"Concentration and inequalities in the financing of obras sociales after deregulation: a comparative analysis of the years 2004 and 2011","resumen":"In Argentina, during the decade of the 1990s major changes were introducedinto the regulatory framework of the national obras sociales, or union-based health coverage plans. Using data from the Federal Administration of Public Income (AFIP) [Administración Federal de Ingresos Públicos], this study evaluates for the years 2004 and 2011: a) the importance of obras sociales within the healthcare system, b) the degree of concentration of this health social security subsystem, and c) the inequalities in the availability of funds among the obras sociales and their beneficiaries. The results show an increased importance of obras sociales within the Argentine health system. The concentration of funds distributed to the most important institutions within the subsystem showed no change, while the concentration of contributors to these institutions slightly increased and that of beneficiaries decreased. Finally, a reduction of the inequalities in funds per beneficiary received by different institutions was observed. This trend can be explained, among other factors, by the attenuation of wage differentials between branches of economic activity and the actions of the so-called Solidarity Redistribution Fund","palabras_clave":"Social Security Agencies|Health Care Rationing|Government Regulation|Argentine"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6468,"idioma_id":4,"titulo":"Current Primary Health Care practices and research challenges in Latin America","resumen":"Since the eighties, most Latin American (LA) countries experienced important social and health improvements as well as profound socio-epidemiological and cultural changes. During this period, the Primary health care (PHC) has been at the core of many reforms. The reforms, with different levels of success, tried to shift from a selective PHC approach to a comprehensive PHC approach. In this context PHC was called upon to address a wider range of problems including the long-standing challenges of still-uncontrolled infectious diseases and maternal-child health, other previously-overlooked problems, such as chronic diseases, traffic accidents, social and domestic violence, mental and oral health, and problem drug use and emerging problems such as HIV, H1N1 and Zika virus. This article presents a special issue of the Family Practice: the International Journal for Research in Primary Care that addresses central challenges of PHC in the LA Region. This issue includes studies from 13 countries of LA. There are two groups of articles: one that tackles analysis of quality and effectiveness of PHC in different health systems, services and programs and a second group made up of studies that analyze the political, institutional and organizational determinants of the PHC development.While the articles joint by this issue along with the existent body of PHC have produced significant scientific contributions, PHC research in LA is still limited and insufficiently guided by the countries? priorities, rules and its health systems and population characteristics.","palabras_clave":"Primary Health Care|Health Services Research|Latin America|Healthcare Reform"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6469,"idioma_id":4,"titulo":"Comparing the performance of the public, social security and private health sub-systems in Argentina by core dimensions of primary health care","resumen":"Background: Most Latin American ?health systems? are comprised by public (PubS), social security (SSS) and private (PrS) sub-systems. These sub-systems coexist, causing healthcare fragmentation and population segmentation.Objective: To estimate the extent of sub-system cross-coverage in a geographically-bounded population (Rosario city) and to compare the sub-systems? performance on primary health care (PHC) dimensions.Methods: Through a cross-sectional interviewer-administered survey to a representative sample (n=822) of the Rosario population, we measured the percentage of cross-coverage (persons with usual source of care from one sub-system but also covered by another sub-system) and the health services? performance by core PHC dimensions, as reported by each sub-system?s usual users. We compared the sub-systems? performance using Chi-Square analysis and one-way ANOVA testing. We analyzed whether the observed differences were coherent with the predominant institutional and organizational features of each sub-system.Results: Overall, 39.3% of the population was affiliated with the PubS, 44.8% with the SSS and 15.9% with the PrS. Cross-coverage was reported by 40.6% of respondents. The performance of the PubS was weak on accessibility but strong on person-and-community-oriented care, the opposite of the PrS. The SSS combined the strengths of the other two sub-systems.Conclusion: Rosario?s health system has a high percentage of cross-coverage, denoting issues of fragmentation, segmentation, financial inequity and inefficiency. The overall performance of the SSS was better than that of the PrS and PubS, though each sub-system had a particular performance pattern with areas of strength and weakness, consistent with their institutional and organizational profiles.","palabras_clave":"Primary Health Care|Health Care Quality, Access, and Evaluation|Integrated Health Care Systems|Health Services Research"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6470,"idioma_id":4,"titulo":"Analysis of the enablers of capacities to produce PHC-based reforms in Latin America: a multiple case study","resumen":"Background: Primary Health Care (PHC) based reforms have had different results in Latin America. Little attention has been paid to the enablers of collective action capacities required to produce a comprehensive PHC approach.Objective: To analyze the enablers of collective action capacities to transform health systems towards a comprehensive PHC approach in Latin American PHC-based reforms.Methods: We conducted a longitudinal, retrospective case study of three municipal PHC-based reforms in Bolivia and Argentina. We used multiple data sources and methodologies: document review; interviews with policy makers, managers and practitioners; household and services surveys. We used temporal bracketing to analyze how the dynamic of interaction between the institutional reform process and the collective action characteristics enabled or hindered the enablers of collective action capacities required to produce the envisioned changes.Results: The institutional structuring dynamics and collective action capacities were different in each case. In Cochabamba, there was an ?interrupted? structuring process that achieved the establishment of a primary level with a selective PHC approach. In Vicente López, there was a ?path-dependency? structuring process that permitted the consolidation of a ?primary care? approach, but with limited influence in hospitals. In Rosario, there was a ?dialectic? structuring process that favored the development of the capacities needed to consolidate a comprehensive PHC approach that permeates the entire system.Conclusion: The institutional change processes achieved the development of a primary healthcare level with different degrees of consolidation and system-wide influence given how the characteristics of each collective action enabled or hindered the ?structuring? processes.","palabras_clave":"Primary Health Care|Healthcare Reform|Politics|Organizational Innovation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6471,"idioma_id":4,"titulo":"Five-year records of Total Mercury Deposition flux at GMOS sites in the Northern and Southern Hemispheres","resumen":"The atmospheric deposition of mercury (Hg) occurs via several mechanisms including dry and wet scavenging by precipitation events. In an effort to understand the atmospheric cycling and seasonal depositional characteristics of Hg, wet deposition samples were collected for approximately five years at 17 selected GMOS monitoring sites located in the Northern and Southern Hemispheres in the framework of the Global Mercury Observation System (GMOS) project. Total mercury (THg) exhibited annual and seasonal patterns in Hg wet deposition samples. Inter-annual differences in total wet deposition are mostly linked with precipitation volume, with the greatest deposition flux occurring in the wettest years. This data set provides a new insight into baseline concentrations of THg concentrations in precipitation worldwide, particularly in regions, such as the Southern Hemisphere and tropical areas where wet deposition as well as atmospheric Hg species were not investigated before, opening the way for future and additional simultaneous measurements across the GMOS network as well as new findings in future modeling studies.","palabras_clave":"MERCURY|ATMOSPHERIC DEPOSITION|5 YEAR RECORDS|GLOBAL TRENDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6472,"idioma_id":4,"titulo":"Electrochemical polymerization of EDOT modified phthalocyanines and their applications as electrochromic materials with green coloration, and strong absorption in the Near-IR","resumen":"Electropolymerization by cyclic voltammetry of both Cu and Zn EDOT modified phthalocyanines is described. Polymeric films were successfully formed on Pt, ITO, and Pt containing a previously deposited layer of PEDOT. Based on electrochemical and spectroelectrochemical studies, a possible polymerization mechanism and a polymer structure are proposed. The films showed a green coloration in the reduced state and a grey coloration and also a very broad band that extends to the NIR zone in the fully oxidized state. Optical contrast ratios of 24% at 650 nm, and of 60% at 1050 nm were achieved, with very fast switching times. A window-type device was also constructed, demonstrating the possible applications of these electrochromic materials in smart windows, which could help in the control of light in the visible and in the control of heat flux because of their strong absorption in the NIR range.","palabras_clave":"PHTHALOCYANINES|ELECTROPOLYMERIZATION|GREEN POLYMER|NIR ABSORPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6473,"idioma_id":5,"titulo":"Análisis de Redes Sociales (ARS) para la investigación en artes audiovisuales y gestión cultural","resumen":"EL uso del Análisis de Redes Sociales como herramienta de gestión. Dentro de la museología, es un dato muy importante conocer como es la red de museos, públicos y privados de una área geográfica determinada. Esa red está conformada por los flujos de bienes y servicios que comparten y mediante el ARS puede ser clarificada con cierta plausibilidad","palabras_clave":"ARS|gestion|cultura|arte"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6474,"idioma_id":5,"titulo":"LA INFRAESTRUCTURA VERDE Y SU PAPEL EN EL DESARROLLO REGIONAL. APLICACIÓN A LOS EJES RECREATIVOS Y CULTURALES DE RESISTENCIA Y SU AREA METROPOLITANA.","resumen":"El presente estudio reúne sintéticamente las principales consideraciones del trabajo realizado en torno a la infraestructura verde y su papel en el desarrollo regional. El objetivo de la investigación fue definir los conceptos básicos de partida e identificar las iniciativas sobre la infraestructura verde, como elemento clave en la definición de un modelo territorial sustentable a nivel regional, aplicándolo a la ciudad de Resistencia, ciudad capital y cabecera del AMGR.&nbsp; Realizando una propuesta para los ejes verdes con funciones recreativas y culturales como elementos integrantes de la infraestructura verde del área en estudio.&nbsp;","palabras_clave":"corredores verdes|verde urbano|planificacion territorial"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6475,"idioma_id":4,"titulo":"Immunization with BLS-Stx2B chimera totally protects dams from early  pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring","resumen":"&lt;!-- \/* Font Definitions *\/@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:'';margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:Calibri;mso-bidi-font-family:'Times New Roman';mso-ansi-language:ES-AR;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Cambria;mso-ascii-font-family:Cambria;mso-fareast-font-family:Cambria;mso-hansi-font-family:Cambria;}@page WordSection1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&gt;&lt;!-- \/* Font Definitions *\/@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:'Cambria Math';panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;}@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:'';margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:Calibri;mso-bidi-font-family:'Times New Roman';mso-ansi-language:ES-AR;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Cambria;mso-ascii-font-family:Cambria;mso-fareast-font-family:Cambria;mso-hansi-font-family:Cambria;}@page WordSection1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&gt;Shiga toxin producing Escherichia coli (STEC) are bacterial pathogens involved infood-borne diseases. Shiga toxin (Stx) is the main virulence factor of STEC andis responsible for systemic complications including Hemolytic Uremic Syndrome(HUS). It has been previously demonstrated that Shiga toxintype 2 (Stx2) induces pregnancy loss in rats in early stage of pregnancy. Themain purpose of this study was to determine if an active immunization prevents Stx2mediated pregnancy loss and confers passive protective immunity to theoffspring. For that purpose Sprague Dawley female rats were immunized with thechimera based on the enzyme lumazine synthase from Brucella spp. (BLS) and the B subunit of Shiga toxin 2 (Stx2B)named BLS-Stx2B. After immunization females were mated with males. At day 8 ofgestation, dams were challenged intraperitoneally with a sublethal and abortifacientdose of Stx2. The immunization induced high anti-Stx2B-specific antibody titersin sera and most important, prevented pregnancy loss. Pups born and breastfeedby immunized dams had high anti-Stx2B-specific antibody titers in sera. Cross-fosteringexperiments indicated that passive protective immunity against Stx2 wastransmitted through lactation. These results indicate that immunizationof adult female rats with BLS-Stx2B prevents Stx2-induced pregnancy loss andconfers anti Stx2 protective immunity to the offspring.","palabras_clave":"Vaccine|pregnancy|Shiga toxin|BLS-Stx2"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6476,"idioma_id":4,"titulo":"Development of the Vertebral Column and Caudal Skeleton in Prochilodus lineatus Larvae under Laboratory Conditions","resumen":"For successful fish larviculture thorough studies describing the development of fish in different morphological aspects are required, as they are crucial for larval survival and growth. The present study described in Prochilodus lineatus larvae the osteological development of the vertebral column and caudal skeleton 30 days after hatching (dah). Larvae were obtained by artificial induction of adults. The beginning of formation of the spine occurs between 10 to 12 dah (8.3 mm standard length, SL) simultaneously to the first neural and hemal processes and the pre-caudal vertebral bodies. The ossification of the vertebral column occurred in cranio- caudal direction and was completed at 28 dah (22.6 mm SL). The development of the caudal skeleton elements started between 6 and 8 dah with the formation of the hypurals (H), the parahipural (PH) and the primary and secondary caudal rays. H 1 to H 3 were formed as cartilaginous primordia on the ventral side of the distal portion of the notochord, while the PH and H 4 to H 6 were formed subsequently. The first rays of the caudal fin were observed in correspondence with the formation of H 2 and H 3, while complete formation of the caudal fin was observed at 28 dah. The epurals, three in number, were evident as cartilaginous elements located both dorsal and distal in the notochord. Central ural complex (CUC) was formed by the fusion of three structures, the center preural 1 and urals 1 and 2. Development of the vertebral column and the caudal skeleton in P. lineatus larvae showed similar patterns to those described for other teleosts.","palabras_clave":"PROCHILODUS|LARVICULTURE|OSTEOLOGICAL DEVELOPMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6477,"idioma_id":4,"titulo":"Prostate Cancer, High Cortisol Levels and Complex Hormonal Interaction","resumen":"Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess prognostic factors and whether high cortisol levels and complex hormonal interactions could be responsible for PCa development. We evaluated the relationship between cortisol, leptin and estrogens in 141 men, 71 with PCa and the remaining 70 constituting a low risk group (LRG). They were recruited for this study from a total of 2906 middle-aged men (ages 45-70 years) who completed an evaluation for prostatic diseases at the Urology Division, Hospital de Clínicas ?José de San Martín?, University of Buenos Aires, in May 2009. In this cross sectional study, cortisol, PSA, total-testosterone, free-testosterone, bioavailable testosterone, LH and estradiol were measured in serum. We observed increased cortisol levels in PCa patients as compared to LRG cases (p=0.004,). Leptin and estradiol levels were also higher in PCa patients (p=0.048; p<0.0001, respectively). Logistic regression analysis indicated that serum cortisol (OR: 1.110 (95% CI 1.016-1.213), p=0.022), estradiol (OR: 1.044 (95% CI 1.008-1.081), p=0.016) and leptin (OR: 1.248 (95% CI 1.048-1.487), p=0.013) explained 27% of the variance of dependent variables, even after adjusting for age, smoking, BMI and waist circumference. We found increased cortisol levels in PCa patients as compared to LRG, as well as an altered circulating hormonal profile.","palabras_clave":"CORTISOL|LEPTIN|PROSTATE CANCER|SEX HORMONES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6478,"idioma_id":4,"titulo":"Heteromerization Between the Bradykinin B2 Receptor and the Angiotensin-(1-7) Mas Receptor: Functional Consequences","resumen":"Bradykinin B2 receptor (B2R) and angiotensin-(1?7) Mas receptor (MasR)?mediated effects are physiologically interconnected. The molecular basis for such cross talk is unknown. It is hypothesized that the cross talk occurs at the receptor level. We investigated B2R?MasR heteromerization and the functional consequences of such interaction. B2R fused to the cyan fluorescent protein and MasR fused to the yellow fluorescent protein were transiently coexpressed in human embryonic kidney293T cells. Fluorescence resonance energy transfer analysis showed that B2R and MasR formed a constitutive heteromer, which was not modified by their agonists. B2R or MasR antagonists decreased fluorescence resonance energy transfer efficiency, suggesting that the antagonist promoted heteromer dissociation. B2R?MasR heteromerization induced an 8-fold increase in the MasR ligand-binding affinity. On agonist stimulation, the heteromer was internalized into early endosomes with a slower sequestration rate from the plasma membrane, compared with single receptors. B2R?MasR heteromerization induced a greater increase in arachidonic acid release and extracellular signal?regulated kinase phosphorylation after angiotensin-(1?7) stimulation, and this effect was blocked by the B2R antagonist. Concerning serine\/threonine kinase Akt activity, a significant bradykinin-promoted activation was detected in B2R?MasR but not in B2R-expressing cells. Angiotensin-(1?7) and bradykinin elicited antiproliferative effects only in cells expressing B2R?MasR heteromers, but not in cells expressing each receptor alone. Proximity ligation assay confirmed B2R?MasR interaction in human glomerular endothelial cells supporting the interaction between both receptors in vivo. Our findings provide an explanation for the cross talk between bradykinin B2R and angiotensin-(1?7) MasR?mediated effects. B2R?MasR heteromerization induces functional changes in the receptor that may lead to long-lasting protective properties.","palabras_clave":"MAS RECEPTOR|B2 RECEPTOR|HETEROMERIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6479,"idioma_id":4,"titulo":"Phylogenetic inference based on landmark data in 41 empirical data sets","resumen":"The inference of phylogenetic hypotheses from landmark data has been questioned during the last two decades. Besides theoretical concerns, one of the limitations pointed out for the use of landmark data in phylogenetics is its (supposed) lack of information relevant to the inference of phylogenetic relationships. However, empirical analyses are scarce; there exists no previous study that systematically evaluates the phylogenetic performance of landmark data in a series of data sets. In the present study, we analysed 41 published data sets in order to assess the correspondence between the phylogenetic trees derived from landmark data and those obtained with alternative and independent sources of evidence, and determined the main factors that might affect this inference. The data sets presented a variable number of terminals (5?200) and configurations (1?14), belonging to different taxonomic groups. The results showed that for most of the data sets analysed, the trees derived from landmark data presented a low correspondence with the reference phylogenies. The results were similar irrespective of the phylogenetic method considered. Complementary analyses strongly suggested that the limited amount of evidence included in each data set (one or a few landmark configurations) is the main cause for that low correspondence: the phylogenetic analysis of eight data sets that presented three or more configurations clearly showed that the inclusion of several landmark configurations improves the results. In addition, the analyses indicated that the inclusion of landmark data from different configurations is more important than the inclusion of more landmarks from the same configuration. Based on the results presented here, we consider that the poor results previously obtained in phylogenetic analyses based on landmark data were not caused by methodological limitations, but rather due to the limited amount of evidence included in the data sets.","palabras_clave":"Landmarks|phylogeny|geometric morphometrics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6480,"idioma_id":4,"titulo":"Typification of the names associated with Anogramma lorentzii (Pteridaceae: Pteridoideae)","resumen":"The genus Anogramma Link (1841: 137) comprises about six species of tropical and temperate regions of America, Ascension Island, southern Europe and Africa east to New Zealand (Tryon & Tryon 1982). The genus is distinguished especially by the annual habit of the sporophyte and the persistence of the gametophyte by means of tubercles.","palabras_clave":"ANOGRAMMA|PTERIDACEAE|TAXONOMY|PTERIDOPHYTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6481,"idioma_id":4,"titulo":"Comparative morphology and anatomy of the leaf and stem of species of Zanthoxylum (Rutaceae) from central Argentina","resumen":"Zanthoxylum is one of the major genera ofRutaceae with at least 200 species in tropicaland temperate regions worldwide. InArgentina is represented by 10 species, twoof them inhabiting in the central region, thenative Zanthoxylum coco and the naturalisedZanthoxylum armatum var. armatum. Thesespecies are found in xerophytic deciduousforests of Chaco biogeographic province.Besides the floristic and ethnobotanicalimportance of these species, their leavesand stems have been scarcely studied. Theobjective of this contribution was to presenta comparative morphological and anatomicalstudy of the leaves and young stems ofthe species of Zanthoxylum from centralArgentina. The analysis was done using herbariavouchers, freshly collected and fixedmaterial. The species share morphologicalfeatures, as armed stems, leaves alternate,odd-pinnately, 3- to many foliolate. Themain differences are that Z. armatum is anevergreen shrub with axillar inflorescences whereas Z. coco is usually a deciduous treewith terminal inflorescences. Both speciesalso share the presence of secretory cavities,glandular trichomes and hypostomaticleaves. However each species presents particularitiesof the leaves structure that couldbe important and related to the environmentthat the plants develop.","palabras_clave":"ZANTHOXYLUM ARMATUM|ZANTHOXYLUM COCO|XERIC HABITATS|FLORA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6482,"idioma_id":4,"titulo":"Genetic differentiation within and away from the chromosomal rearrangements characterising hybridising chromosomal races of the western house mouse (Mus musculus domesticus)","resumen":"The importance of chromosomal rearrangements for speciation can be inferred from studies of genetic exchange between hybridising chromosomal races within species. Reduced fertility or recombination suppression in karyotypic hybrids has the potential to maintain or promote genetic differentiation in genomic regions near rearrangement breakpoints. We studied genetic exchange between two hybridising groups of chromosomal races of house mouse in Upper Valtellina (Lombardy, Italy), using microsatellites. These groups differ by Robertsonian fusions and\/or whole-arm reciprocal translocations such that F1 hybrids have a chain-of-five meiotic configuration. Previous studies showed genetic differentiation in two chromosomes in the chain-of-five (10 and 12) close to their centromeres (i.e. the rearrangement breakpoints); we have shown here that the centromeric regions of the other two chromosomes in the chain (2 and 8) are similarly differentiated. The internal chromosomes of the chain (8 and 12) show the greatest differentiation, which may reflect pairing and recombination properties of internal and external elements in a meiotic chain. Importantly, we found that centromeric regions of some non-rearranged chromosomes also showed genetic differentiation between the hybridising groups, indicating a complex interplay between chromosomal rearrangements and other parts of the genome in maintaining or promoting differentiation and potentially driving speciation between chromosomal races.","palabras_clave":"Robertsonian fusion|chromosomal hybrid zone|introgression|microsatellites|speciation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6483,"idioma_id":4,"titulo":"Development and characterization of microwave-assisted activated carbons from Parkinsonea aculeata wood","resumen":"Activated carbons from invasive Parkinsonia aculeata wood sawdust were developed by microwave assisted H3PO4 acid activation varying the radiation time (2?10 min). Their main physicochemical characteristics were determined, and compared with those of a sample previously obtained by conventional heating using the same precursor, chemical agent, and impregnation ratio.The activated carbons developed for radiation times of 5 and 10 min showed higher BET surface areas (1300 m2\/g) and total pore volume (1 cm3\/g) than those obtained at 2 min (710 m2\/g) or by conventional heating (970 m2\/g). Microwave assisted activation also led to activated carbons with higher total amounts of acidic oxygen and basic functionalities in comparison with the sample arising from conventional heating. Phenol adsorption capacity for all the samples was also evaluated from determination of the adsorption isotherms and their proper description by the Freundlich model. The adsorption capacity for the activated carbons obtained at 5 and 10 min was almost twice that measured for the samples prepared at 2 min and by conventional heating, in agreement with the higher BET surface areas characterizing the former. Compared with conventional heating, the process assistance by microwave energy not only reduced operation time, but also improved the characteristics of the resulting activated carbons.","palabras_clave":"ACTIVATED CARBON|MICROWAVE ASSISTANCE|H3PO4 ACID ACTIVATION|PHENOL ADSORPTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6484,"idioma_id":4,"titulo":"B. pertussis modulates human macrophage defense gene expression","resumen":"Bordetella pertussis, the etiologic agent of whooping cough, still causes outbreaks. We recently found evidence that B. pertussis can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche for persistence. In this work, we examined the interaction of B. pertussis with a human monocyte cell line (THP-1) that differentiates into macrophages in culture in order to investigate the host cell response to the infection and the mechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selected number of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phases of infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, while the number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of B. pertussis, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cell response. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections by those bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome of the interaction between B. pertussis and macrophages.","palabras_clave":"Bordetella pertussis|intracellular survival|adenylate cyclase|pertussis toxin|host cell defense response"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6485,"idioma_id":5,"titulo":"Bioeconomía Matemática: un aporte al manejo de los recursos naturales","resumen":"El articulo muestra un breve estudio del modelo bioeconómico de Gordon-Schaefer (1954), el cual es fruto de una gran variedad de modelos propuestos en el área bioeconómica durante la segunda mitad del siglo pasado.Se efectúa también un acotado análisis de las ecuaciones diferenciales impulsivas como herramientas matemáticas útiles para modelar algunas interacciones entre especies.","palabras_clave":"BIOECONOMÍA|GORDON SHAEFER |ECUACIONES DIFERENCIALES IMPULSIVAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6486,"idioma_id":4,"titulo":"A comparative evaluation of different ionic liquids for arsenic species separation and determination in wine varietals by liquid chromatography - hydride generation atomic fluorescence spectrometry","resumen":"The application of different ionic liquids (ILs) as modifiers for chromatographic separation and determination of arsenite [As(III)], arsenate [As(V)], dimethylarsonic acid (DMA) and monomethylarsonic acid (MMA) species in wine samples, by reversed-phase high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry detection (RP-HPLC-HG-AFS) was studied in this work. Several factors influencing the chromatographic separation of the As species, such as pH of the mobile phase, buffer solution concentration, buffer type, IL concentration and length of alkyl groups in ILs were evaluated. The complete separation of As species was achieved using a C18 column in isocratic mode with a mobile phase composed of 0.5% (v\/v) 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) and 5% (v\/v) methanol at pH 8.5. A multivariate methodology was used to optimize the variables involved in AFS detection of As species after they were separated by HPLC. The ILs showed remarkable performance for the separation of As species, which was obtained within 18 min with a resolution higher than 0.83. The limits of detection for As(III), As(V), MMA and DMA were 0.81, 0.89, 0.62 and 1.00 &#956;g As L&#8722;1. The proposed method was applied for As speciation analysis in white and red wine samples originated from different grape varieties.","palabras_clave":"Arsenic speciation|Wines|Ionic liquids|Liquid chromatography|Atomic fluorescence spectrometry"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6487,"idioma_id":4,"titulo":"Structured and Unstructured Binding of an Intrinsically Disordered Protein as Revealed by Atomistic Simulations","resumen":"Intrinsically disordered proteins (IDPs) are a set of proteins that lack a definite secondary structure in solution. IDPs can acquire tertiary structure when bound to their partners; therefore, the recognition process must also involve protein folding. The nature of the transition state (TS), structured or unstructured, determines the binding mechanism. The characterization of the TS has become a major challenge for experimental techniques and molecular simulations approaches since diffusion, recognition, and binding is coupled to folding. In this work we present atomistic molecular dynamics (MD) simulations that sample the free energy surface of the coupled folding and binding of the transcription factor c-myb to the cotranscription factor CREB binding protein (CBP). This process has been recently studied and became a model to study IDPs. Despite the plethora of available information, we still do not know how c-myb binds to CBP. We performed a set of atomistic biased MD simulations running a total of 15.6 &#956;s. Our results show that c-myb folds very fast upon binding to CBP with no unique pathway for binding. The process can proceed through both structured or unstructured TS?s with similar probabilities. This finding reconciles previous seemingly different experimental results. We also performed Go-type coarse-grained MD of several structured and unstructured models that indicate that coupled folding and binding follows a native contact mechanism. To the best of our knowledge, this is the first atomistic MD simulation that samples the free energy surface of the coupled folding and binding processes of IDPs.","palabras_clave":"Intrinsically Disordered Proteins|c-myb"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6488,"idioma_id":4,"titulo":"Phosphorylation Regulates the Bound Structure of an Intrinsically Disordered Protein: The p53-TAZ2 Case","resumen":"Disordered regions and Intrinsically Disordered Proteins (IDPs) are involved in critical cellular processes and may acquire a stable three-dimensional structure only upon binding to their partners. IDPs may follow a folding-after-binding process, known as induced folding, or a folding-before-binding process, known as conformational selection. The transcription factor p53 is involved in the regulation of cellular events that arise upon stress or DNA damage. The p53 domain structure is composed of an N-terminal transactivation domain (p53TAD), a DNA Binding Domain and a tetramerization domain. The activity of TAD is tightly regulated by interactions with cofactors, inhibitors and phosphorylation. To initiate transcription, p53TAD binds to the TAZ2 domain of CBP, a co-transcription factor, and undergoes a folding and binding process, as revealed by the recent NMR structure of the complex. The activity of p53 is regulated by phosphorylation at multiple sites on the TAD domain and recent studies have shown that modifications at three residues affect the binding towards TAZ2. However, we still do not know how these phosphorylations affect the structure of the bound state and, therefore, how they regulate the p53 function. In this work, we have used computational simulations to understand how phosphorylation affects the structure of the p53TAD:TAZ2 complex and regulates the recognition mechanism. Phosphorylation has been proposed to enhance binding by direct interaction with the folded protein or by changing the unbound conformation of IDPs, for example by pre-folding the protein favoring the recognition mechanism. Here, we show an interesting turn in the p53 case: phosphorylation mainly affects the bound structure of p53TAD, highlighting the complexity of IDP protein-protein interactions. Our results are in agreement with previous experimental studies, allowing a clear picture of how p53 is regulated by phosphorylation and giving new insights into how post-translational modifications can regulate the function of IDPs.","palabras_clave":"Intrinsically Disordered Proteins|Phosphorylation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6489,"idioma_id":5,"titulo":"Conflictos en extensión: origen y posibles estrategias de abordaje","resumen":"La extensión universitaria es concebida desde diferentes miradas y se actúa sobre la base de esos posicionamientos; esa pluralidad puede estar contenida en los propios equipos de extensionistas y en los sectores de la sociedad con quienes éstos interactúan. Esto genera expectativas y actitudes de las partes involucradas que pueden provocar conflictos. Se presentan y analizan casos de tensiones sobre la base de experiencias de trabajo en extensión, así como el planteo de propuestas, como estrategias de resolución de conflictos.","palabras_clave":"articulación universidad - sociedad|manejo de grupos|manejo de conflictos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6490,"idioma_id":4,"titulo":"iRGD peptide conjugation potentiates intraperitoneal tumor delivery of paclitaxel with polymersomes","resumen":"Polymersomes are versatile nanoscale vesicles that can be used for cytoplasmic delivery of payloads. Recently, we demonstrated that pH-sensitive polymersomes exhibit an intrinsic selectivity towards intraperitoneal tumor lesions. A tumor homing peptide, iRGD, harbors a cryptic C-end Rule (CendR) motif that is responsible for neuropilin-1 (NRP-1) binding and for triggering extravasation and tumor penetration of the peptide. iRGD functionalization increases tumor selectivity and therapeutic efficacy of systemic drug-loaded nanoparticles in many tumor models. Here we studied whether intraperitoneally administered paclitaxel-loaded iRGD-polymersomes show improved efficacy in the treatment of peritoneal carcinomatosis. First, we demonstrated that the pH-sensitive polymersomes functionalized with RPARPAR (a prototypic CendR peptide) or iRGD internalize in the cells that express NRP-1, and that internalized polymersomes release their cargo inside the cytosol. CendR-targeted polymersomes loaded with paclitaxel were more cytotoxic on NRP-1-positive cells than on NRP-1-negative cells. In mice bearing peritoneal tumors of gastric (MKN-45P) or colon (CT26) origin, intraperitoneally administered RPARPAR and iRGD-polymersomes showed higher tumor-selective accumulation and penetration than untargeted polymersomes. Finally, iRGD-polymersomes loaded with paclitaxel showed improved efficacy in peritoneal tumor growth inhibition and in suppression of local dissemination compared to the pristine paclitaxel-polymersomes or Abraxane.Our study demonstrates that iRGD-functionalization improves efficacy of paclitaxel-polymersomes for intraperitoneal treatment of peritoneal carcinomatosis.","palabras_clave":"Polymersomes|Tumor penetrating peptides|Peritoneal carcinomatosis|Paclitaxel|iRGD|NRP-1"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6491,"idioma_id":4,"titulo":"Highly crystalline LiCu X Fe 1&#8722;X PO 4 nanoparticles synthesized by high temperature thermal decomposition: a morphological and electrical transport study","resumen":"In this work, we report the morphological and electrical characterization of highly crystalline LiCuXFe1&#8722;XPO4 nanoparticles synthesized via the high-temperature (380 °C) thermal decomposition of organometallic precursors. The mean diameter of the studied nanoparticles was 30?40 nm. The Cu\/Fe relations of 0, 0.001 and 0.042 for the three studied samples were obtained via particle-induced x-ray emission spectroscopy. Crystallographic and morphological studies were performed using x-ray diffraction, transmission electron microscopy and high-resolution transmission electron microscopy techniques. We investigated the effects of incorporating copper on the electric transport properties of this highly crystalline nanometric system using impedance spectroscopy and DC transport techniques. The experimental evidence allowed us to conclude that in the frequency range f &lt; 1 kHz the transport is dominated by the diffusion of Li and the presence of Cu atoms in the systems hinders this transport mechanism, despite the high crystallinity of the system.","palabras_clave":"NANOPARTICLES|IMPEDANCE SPECTROSCOPY|Li-ION BATTERIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6492,"idioma_id":4,"titulo":"Neutron dose estimation in a zero power nuclear reactor","resumen":"Estimación de dosis neutrónica en geles irradiados en el Reactor Nuclear RA-0 de la Universidad Nacional de Córdoba.","palabras_clave":"Dosimetros|Geles|Radiaciones|Neutrón"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6493,"idioma_id":5,"titulo":"Primer registro de una puesta de Tropidurus spinulosus (Squamata: Tropiduridae) en el Chaco Argentino","resumen":"Se reporta por primera ves las características de una puesta de huevos de Tropidurus spinulosus en estado natural en un bosque de Quebracho colorado en una localidad de la provincia del Chaco en Argentina","palabras_clave":"huevos|lagartos espinosos|Chaco|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6494,"idioma_id":5,"titulo":"ANÁLISIS DE LA LONGITUD DE LOS TELÓMEROS EN OBESOS METABÓLICAMENTE SANOS","resumen":"La obesidad es el principal componente del Síndrome Metabólico (SM) y determina la progresión de la enfermedad a las complicaciones metabólicas. Los individuos obesos metabólicamente sanos (OMS) parecen estar protegidos contra esas complicaciones. La longitud de los telómeros (LT) es un nuevo marcador del envejecimiento celular, que tiene una relación compleja con el SM. El objetivo principal de este estudio fue investigar por primera vez la LT en OMS, y estudiar la asociación entre LT y el número de componentes del SM.Se estudiaron 398 mujeres con una edad media de 46.76 ± 15,47 años (rango: 18 - 86 años), que se agruparon en: individuos normo-peso sin ningún componente del SM (NP0), obesos sin SM (OMS), y de acuerdo al número de componentes de SM en los grupos sin ningún componentes de SM (0), con uno o dos componentes (1+2) y con SM por la presencia de 3 o más componentes (SM).La LT de los OMS no se diferenció de la de los NP0, pero fue significativamente mayor que la de los individuos con SM (p = 0,032). Se observó una disminución de la LT con el aumento progresivo del número de componentes del SM (p = 0,004), en donde el grupo 0 presentó una LT significativamente mayor que los grupos 1+2 (p = 0,027) y SM (p = 0,003).Demostramos por primera vez que las mujeres OMS presentan una LT similar a las mujeres NP0, y más larga que aquellas mujeres con SM. Además confirmamos que la LT se acorta con el aumento en el número de alteraciones del SM.","palabras_clave":"Longitud telomérica|Obesos metabólicamente sanos|Síndrome metabólico"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6495,"idioma_id":4,"titulo":"Molecular and morphological insights into the origin of the invasive greater white-toothed shrew (Crocidura russula) in Ireland","resumen":"Identifying routes of invasion is a critical management strategy in controlling the spread of invasive species. This is challenging however in the absence of direct evidence. Therefore, indirect methodologies are used to infer possible invasion sources and routes, such as comparisons of genetic and morphological data from populations from invasive ranges and putative source areas. The greater whitetoothed shrew (Crocidura russula) was first discoveredin Ireland from skeletal remains in the pellets of birds of prey collected in 2007 and is it is now sufficiently established that the species has a detrimental impact on Ireland?s small mammal community. In this study, we address the uncertain origin(s) of the Irish population of C. russula. The cytochrome b gene of mitochondrial DNA was analysed from 143 individuals from throughout its range within a phylogenetic and approximate Bayesian computationframework. These analyses revealed that the Irish population stemmed from Europe as opposed to North Africa. Additionally, mandibles from 523 individualsfrom Ireland and 28 other European populations were subjected to multivariate and distance-based analyses, which demonstrated an association between the Irishpopulation and those in France, Switzerland and Belgium. When the genetic and morphological analyses were considered together, an origin stemming from France was deemed the most likely scenario for the source of the invasive Irish population. This study has demonstrated the importance of utilising a multidisciplinary approach when attempting to identify the origins and invasion routes of invasive species.","palabras_clave":"Approximate Bayesian Computation|Cytochrome b|Europe|Mandibles|Mitochondrial DNA|Morphometrics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6496,"idioma_id":4,"titulo":"Methods for the detection of reactive oxygen species employed in the identification of plant photosensitizers","resumen":"Over the past ten years, alternative methods for the rapid screening of PSs have been developed. In the present work, a study was undertaken to correlate the phototoxicity of plant extracts on either prokaryotic or eukaryotic cells, with the total oxidation status (TOS) as well as with their ability to produce 1O2.Results demonstrated that the extracts containing PSs that were active either on eukaryotic cells or bacteria increased their TOS after illumination, and that there was a certain degree of positive correlation between the extract phototoxic efficacy and TOS levels.The production of 1O2 by the illuminated extracts was indirectly measured by the use of the fluorescence of ??singlet oxygen sensor green?, which is a method that has proved highly sensitive for such measurement. 1O2 was detectable only upon illumination of the most active extracts.In addition, the oxidation of tryptophan and was employed as a method capable of measuring ROS generated by both type I and II ROS reactions. However, it turned out to be not sensitive enough to detect the species generated by plant extracts.Results demonstrated that the TOS method, initially developed to measure the oxidant status in plasma, can be readily applied to plant extracts. Unlike the method used to detect 1O2, the method employed for the detection of TOS proved to be accurate, since all the extracts that displayed a high phototoxic activity on either prokaryotic or eukaryotic cells, presented high TOS levels after illumination.","palabras_clave":"methods|free radicals|singlet oxygen|plants"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6497,"idioma_id":4,"titulo":"Reference Intervals of Mineral Elements in Plasma of Anesthetized Free-Ranging Adult Females of South American Sea Lion, Otaria flavescens","resumen":"Mineral elements are inorganic substances some present in body tissues and fluids necessary for the maintenance of certain physicochemical processes essential to life. Pinnipeds,like other marine mammal species, obtain their required minerals primarily from the diet. Often, diseases are associated with an imbalance of the electrolyte homeostasis causing changes in blood chemistry.Therefore, reference intervals are a critical diagnostic tool used in the care of stranded animals and for population health investigations. In marine mammals from natural colonies, the information about essential elements in blood fractions is limited, and most reports refer to whole blood.","palabras_clave":"Otaria flavescens| Blood | Minerals elements"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6498,"idioma_id":4,"titulo":"The wind of rotating B supergiants. I. Domains of slow and fast solution regimes","resumen":"In the scenario of rotating radiation-driven wind theory for massive stars, three types of stationary hydrodynamic solutions are currently known: the classical ( fast) m-CAK solution, the &#937;-slow solution that arises for fast rotators, and the so-called &#948;-slow solution if high values of the &#948; line-force parameter are allowed independently of the rotation speed. Compared to the fast solution, both ?slow solutions? have lower terminal velocities. As the study ofthe parameter domain for the slow solution is still incomplete, we perform a comprehensive analysis of the distinctive flow regimes for B supergiants that emerge from a fine grid of rotation values, &#937;, and various ionizationconditions in the wind (&#948;) parameter. The wind ionization defines two domains: one for fast outflowing winds and the other for slow expanding flows. Both domains are clear-cut by a gap, where a kink\/plateau structure of thevelocity law could exist for a finite interval of &#948;. The location and width of the gap depend on Teff and &#937;. There is a smooth and continuous transition between the &#937;-slow and &#948;-slow regimes, a single &#937; &#948;-slow regime. We discussdifferent situations where the slow solutions can be found and the possibility of a switch between fast and slow solutions in B supergiant winds. We compare the theoretical terminal velocity with observations of B and Asupergiants and find that the fast regime prevails mostly for early B supergiants while the slow wind regime matches better for A and B mid- and late-type supergiants.","palabras_clave":"Hydrodynamics |Stars: early-type|Stars: mass-loss|Stars: winds, outflows"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6500,"idioma_id":4,"titulo":"Endocannabinoids participate in placental apoptosis induced by hypoxia inducible factor - 1","resumen":"During pregnancy, apoptosis is a physiological event critical in the remodeling and aging of the placenta. Increasing evidence has pointed towards the relevance of endocannabinoids (ECs) and hypoxia as modulators of trophoblast cell death. However, the relation between these factors is still unknown. In this report, we evaluated the participation of ECs in placental apoptosis induced by cobalt chloride (CoCl2), a hypoxia mimicking agent that stabilizes the expression of hypoxia inducible factor-1 alpha (HIF-1&#945;). We found that HIF-1 stabilization decreased FAAH mRNA and protein levels, suggesting an increase in ECs tone. Additionally, CoCl2 incubation and Met-AEA treatment reduced cell viability and increased TUNEL-positive staining in syncytiotrophoblast layer. Immunohistochemical analysis demonstrated Bax and Bcl-2 protein expression in the cytoplasm of syncytiotrophoblasts. Finally, HIF-1 stabilization produced an increase Bax\/Bcl-2 ratio, activation of caspase 3 and PARP cleavage. All these changes in apoptotic parameters were reversed with AM251, a CB1 antagonist. These results demonstrate that HIF-1 may induce apoptosis in human placenta via intrinsic pathway by a mechanism that involves activation of CB1 receptor suggesting a role of the ECs in this process.","palabras_clave":"Placenta|Apoptosis|Endocannabinoides|HIF"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6501,"idioma_id":4,"titulo":"A gigantic new dinosaur from Argentina and the evolution of the sauropod hind foot","resumen":"Titanosauria is an exceptionally diverse, globally-distributed clade of sauropod dinosaurs that includes the largest known land animals. Knowledge of titanosaurian pedal structure is critical to understanding the stance and locomotion of these enormous herbivores and, by extension, gigantic terrestrial vertebrates as a whole. However, completely preserved pedes are extremely rare among Titanosauria, especially as regards the truly giant members of the group. Here we describe Notocolossus gonzalezparejasi gen. et sp. nov. from the Upper Cretaceous of Mendoza Province, Argentina. With a powerfully-constructed humerus 1.76 m in length, Notocolossus is one of the largest known dinosaurs. Furthermore, the complete pes of the new taxon exhibits a strikingly compact, homogeneous metatarsus?seemingly adapted for bearing extraordinary weight?and truncated unguals, morphologies that are otherwise unknown in Sauropoda. The pes underwent a near-progressive reduction in the number of phalanges along the line to derived titanosaurs, eventually resulting in the reduced hind foot of these sauropods (18) A gigantic new dinosaur from Argentina and the evolution of the sauropod hind foot. Available from: https:\/\/www.researchgate.net\/publication\/291136592_A_gigantic_new_dinosaur_from_Argentina_and_the_evolution_of_the_sauropod_hind_foot [accessed Jul 01 2018].","palabras_clave":"Notocolossus|Articulated pes|Titanosaurian|Mendoza"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6502,"idioma_id":5,"titulo":"Descomposición Térmica Del Diperóxido Ácido De Glutaraldehído En Metanol Para Determinar Parámetros De Activación Y Mecanismo De Reacción","resumen":"En este trabajo se informan los resultados obtenidos en el estudio de la descomposición térmica del diperóxido ácido de glutaraldehído (DPAG) en solución de metanol, a fin de determinar los parámetros de activación correspondientes y dilucidar el mecanismo de reacción, la importancia de este tipo de compuesto se debe a que actualmente se ha comprobado que tienen acción farmacológica como bactericidas, antitumorales y como antimaláricos.La reacción se realizó entre 130,0 - 166,0 ºC y a concentración inicial de 5,49 x 10-4 mol\/L, la misma cumple con una ley cinética de primer orden hasta conversiones del peróxido de ca. 50 %. El análisis de los productos de reacción y los valores del parámetro de activación contribuye a postular que el mecanismo para la termólisis del DPAG en solución de metanol comienza con la ruptura homolítica del enlace peroxídico dando un intermediario birradical, que sufre una ruptura CO dando ácido glutárico y oxígeno.","palabras_clave":"TETROXAN0|ACTIVIDAD|CALCULOS TEORICOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6503,"idioma_id":4,"titulo":"Comparative study of toxicity and biochemical responses induced by sublethal levels of the pesticide azinphosmethyl in two fish species from North-Patagonia, Argentina","resumen":"Biochemical effects of azinphosmethyl (AZM), anorganophosphate pesticide, were determined in gill, brain and muscle tissues ofOdontesthes hatcheri and Jenynsia multidentata. The 96-htoxicity was first assessed, estimating lethal concentrations fifty (LC50) of 7and 30 &#956;g L-1 AZM for O.hatcheri and J. multidentata,respectively. Considering the LC50, sublethal 96-h static exposures weredesigned for O. hatcheri (0.1-0.5 &#956;g L-1AZM) and J. multidentata (5-10 &#956;g L-1AZM)to determine biochemical endpoints. Brain acetylcholinesterase (AchE) was inhibited by AZM in both species, while the buffer enzyme carboxylesterase(CarbE) was not affected in this tissue. Conversely, muscular AchE was notaffected but CarbE was augmented by AZM. The enzymesglutathione reductase, glutathione-S-transferase and CarbE were significantlyinhibited in O. hatcheri gills but none of them was affected by AZM in J. multidentata gills compared tocontrol. GSH levels were augmentedin gills of both species in exposed fish compared to controls and in addition,lipid peroxidation was significantly increased in O. hatcheri gills. Ex vivohistochemical analysis of ROS by fluorescence microscopy was also performed in J. multidentata gills, indicating a significantincrease upon exposure to 10 µg L-1 AZM. Principalcomponent analyses (PCA) were applied, both to the species together orseparately. The general analysis demonstrated a clear separation of responsesin the two species. For O. hatcheri,the variable that explains the major variation in PC1 is gill catalase andbrain AchE in PC2. In J. multidentatain turn, the variable that explains the major variation in PC1 is brain AchEand total oxyradical scavenging capacity in PC2. The toxicity data andbiomarker responses obtained for both species were compared to environmentalconcentrations of AZM detected in superficial water from different points inthe Alto Valle region and risk quotients (RQ) were calculated. This approachindicated probable acute effects for O.hatcheri in river and irrigation channels (RQ > 0.1), while the risk wasunacceptable in drainage superficial water (RQ > 1). In contrast, J. multidentata showed minimal risk inriver or channel water (RQ < 0.1) and probable risk in drainage water (RQ = 0.75).We conclude that not only the differential susceptibility of both species toAZM is environmentally relevant, but also that the different biomarkersresponding in each case underlie particular pathways stressed by this agrochemical.","palabras_clave":"ORGANOPHOSPHATE|OXIDATIVE STRESS |ODONTESTHES HATCHERI |JENYNSIA MULTIDENTATA|RISK ASSESSMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6504,"idioma_id":5,"titulo":"Conocimientos y reflexiones de adultos sobre la numeración escrita","resumen":"En este artículo se presentan datos de un estudio que permitió relevar ciertos conocimientos numéricos de adultos que inician su escolaridad primaria en la Ciudad de Buenos Aires.  Las entrevistas han buscado comprender no solo como estos adultos leen y escriben números, sino fundamentalmente sus ideas implícitas y cómo las explicitan y amplían en momentos de análisis reflexivo sobre sus propias producciones. Los datos relevados permiten interpelar la enseñanza clásica de la numeración y ofrecer algunas intervenciones didácticas.","palabras_clave":"conocimientos matemáticos|adultos no escolarizados"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6505,"idioma_id":4,"titulo":"Alterations in the intestine of Patagonian silverside ( Odontesthes hatcheri) exposed to microcystin-LR: Changes in the glycosylation pattern of the intestinal wall and inhibition of multidrug resistance proteins efflux activity","resumen":"Accumulation and toxicity of cyanobacterial toxins, particularly microcystin-LR (MCLR) have been exten-sively studied in fish and aquatic invertebrates. However, MCLR excretion mechanisms, which couldreduce this toxin?s effects, have received little attention. The Patagonian silverside, Odontesthes hatcheri,is an omnivorous-planktivorous edible fish, which has been shown to digest cyanobacterial cells absorb-ing MCLR and eliminating the toxin within 48 h without suffering significant toxic effects. We studiedthe effects of MCLR on glycoconjugate composition and the possible role of multidrug resistance associ-ated proteins (Abcc) in MCLR export from the cells in O. hatcheri intestine. We treated O. hatcheri with5 g MCLR g&#8722;1body mass administered with the food. Twenty four hours later, the intestines of treatedand control fish were processed for lectin-histochemistry using concanavalin A (ConA), Triticum vulgarisagglutinin (WGA), and Dolichos biflorus agglutinin (DBA). MCLR affected the distribution of glycoconju-gates by augmenting the proportion of ConA-positive at the expense of WGA-positive cells. We studiedMCLR effects on the transport of the Abcc-like substrates 2,4-dinitrophenyl-S-glutathione (DNP-SG) andcalcein in ex vivo intestine preparations (everted and no-everted sacs and strips). In treated preparations,CDNB together with MCLR (113 g MCLR g&#8722;1intestine, equivalent to 1.14 mol L&#8722;1when applied in thebath) or the Abcc inhibitor, MK571 was applied for one hour, during which DNP-SG was measured in thebath every 10 min in order to calculate mass-specific DNP-SG transport rate. MCLR significantly inhibitedDNP-SG transport (p < 0.05), especially in middle intestine (47 and 24%, for luminal and serosal transport,respectively). In middle intestine strips, MCLR and MK571inhibited DNP-SG transport in a concentrationdependent fashion (IC503.3 and 0.6 mol L&#8722;1, respectively). In middle intestine strips incubated withcalcein-AM (0.25 mol L&#8722;1), calcein efflux was inhibited by MCLR (2.3 mol L&#8722;1) and MK571 (3 mol L&#8722;1)by 38 and 27%, respectively (p < 0.05). Finally, middle intestine segments were incubated with differentconcentrations of MCLR applied alone or together with 3 M MK571. After one hour, protein phosphatase1 (PP1) activity, the main target of MCLR, was measured. 2.5 M MCLR did not produce any significanteffect, while the same amount plus MK571 inhibited PP1 activity (p < 0.05). This effect was similar to that of 5 M MCLR. Our results suggest that in O. hatcheri enterocytes MCLR is conjugated with GSH via GST andthen exported to the intestinal lumen through Abcc-like transporters. This mechanism would protect thecell from MCLR toxicity, limiting toxin transport into the blood, which is probably mediated by basolateral Abccs. From an ecotoxicological point of view, elimination of MCLR through this mechanism would reducethe amount of toxin available for trophic transference.","palabras_clave":"ABCC TRANSPORTERS|CYANOTOXIN|GLYCOCONJUGATES|INTESTINAL SACS|LECTIN-HISTOCHEMISTRY|MULTIXENOBIOTIC RESISTANCE|ODONTESTHES HATCHERI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6506,"idioma_id":4,"titulo":"Erysipelothrix rhusiopathiae isolated from gull-inflicted wounds in southern right whale calves","resumen":"Southern right whales Eubalaena australis from Peninsula Valdés, Argentina, show wounds produced by kelp gulls Larus dominicanus that feed on the whales´ dorsal skin and blubber. During the 2013 whale season, several calves were reported showing kelp gull injuries with a swollen area surrounded by rhomboid-shaped raised edges. Samples from 9 calves were taken in order to establish the etiology of these rhomboid-shaped wounds; 2 calves (one living, one dead) showed gull-inflicted injuries with rhomboid-shaped edges. Samples from the dead calf were histologically characterized by the presence of dermal congestion, suppurative dermatitis and panniculitis, necrotizing vasculitis and vascular thrombosis. Erysipelothrix rhusiopathiae was detected by culture and PCR in samples from both calves. In this study we report, for the first time to our knowledge, the isolation of E. rhusiopathiae from wounds produced by gull attacks on southern right whale calves, supplying evidence that these wounds may act as an entry route for pathogens. This work provides new information about the consequences of gull-inflicted injuries for whale health.","palabras_clave":"ERYSIPELOTHRIX RHUSIOPATHIAE|EUBALAENA AUSTRALIS|LARUS DOMINICANUS|SKIN INJURIES|SOUTHERN RIGHT WHALE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6507,"idioma_id":5,"titulo":"Prodromus sinsistemático de la República Argentina y una breve introducción a los estudios fitosociológicos","resumen":"Resumen: Se presenta el Prodromus de la vegetación de Argentina, que constituye la primera versión del sinsistema nacional detallado hasta nivel de asociación y subasociación. Se describen las 57 clases de vegetación hasta ahora registradas para el país y la presencia posible de otras 16. Se indican además las comunidades vegetales sin rango taxonómico. Todas las unidades de vegetación se enmarcan en el esquema fitogeográfico vigente.","palabras_clave":"FITOSOCIOLOGIA|ARGENTINA|VEGETACION|SINTAXONOMIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6508,"idioma_id":5,"titulo":"Especialización y complementación productiva en el MERCOSUR: un Enfoque de Cadenas Productivas de Valor","resumen":"El MERCOSUR ha mostrado un creciente interés por utilizar la integración regional como base para aumentar su competitividad internacional aludiendo a la complementariedad y especialización productiva entre sus socios. Este trabajo pretende realizar una contribución metodológica a la medición de este fenómeno, agregando productos para conformar cadenas productivas de valor, al mismo tiempo que realiza una primera medición de esta nueva metodología para el MERCOSUR durante el período 2003-2012. Los resultados arrojan algunos casos incipientes de integración productiva, fundamentalmente para los socios de mayor tamaño, y varias potencialidades interesantes para la aplicación de una política productiva para el bloque.Referato de Comité Editorial Interno.*Referido por Comité Editorial Interno (IIEP)","palabras_clave":"cadenas productivas|especialización|Mercosur|sustitución de importaciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6509,"idioma_id":5,"titulo":"La salud oral y general de los pobladores de las comunidades de Asunción y San Mi-guel en relación con las condiciones socioambientales, en áreas no irrigadas de Lava-lle","resumen":"En la zona de estudio sólo es productivo un pequeño porcentaje de la superficie total. Hace algo más de cien años era un área cubierta de lagunas que se formaban por los desagües de los Ríos Mendoza y San Juan como se puede ver en el libro ?Guanacache. Fidel Roig Matóns, pintor del desierto?, que fueron transformados en desierto por los usos intensivos de los oasis centrales de las provincias de San Juan y Mendoza. Además existían grandes bosques de algarrobo, que fueron talados para durmientes de los trenes, carbón cuando existían los trenes a vapor y luego para postes de viñedos. Los objetivos son el estudio de la salud oral y salud general de los pobladores de las comunidades de Asunción y San Miguel, teniendo en cuenta características desde lo social antropológico, y asociarlas con problemáticas como la ambiental, las culturales, la relación con la tierra, la población originaria (pueblos huarpes), el agua, la educación, entre otros aspectos.","palabras_clave":"SALUD |CONDICIONES AMBIENTALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6510,"idioma_id":4,"titulo":"Pleiotropic effects of 5-aminolevulinic acid in mouse brain","resumen":"5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychyatric manifestations of Acute Intermittent Porphyria (AIP). The aim was to study the effect of ALA on different metabolisms in mice brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, cholinergic system, defense enzyme system and nitric oxide metabolism were evaluated in encephalon mice receiving a single (40 mg\/kg) or multiple doses of ALA (40 mg\/kg, 14 days). We have found ALA accumulation in encephalon. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in Superoxide dismutase activity and a reduction of glutathione levels were detected, while malondialdehyde levels and Catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect this organ against the injury. All Nitric Oxide Synthase isoforms were induced by ALA, being these changes more significant in glial cells for the inducible isoform. In conclusion, ALA affected several metabolisms in encephalon. Data indicate that a rapid response to oxidative stress was developed; however, in long term intoxication, the redox balance was probably restored minimizing oxidative damage.","palabras_clave":"ALA|mouse_brain|5_alfa_aminolevulic_acid|Nitric_oxidase_sintetase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6511,"idioma_id":4,"titulo":"Analysis of the Molecular Evolution of Hepatitis B Virus Genotypes in Symptomatic Acute Infections in Argentina","resumen":"Hepatitis B virus (HBV) is a globally distributed human pathogen that leads to both self-limited and chronic infections. At least eight genotypes (A-H) with distinct geographical allocations and phylodynamic behaviors have been described. They differ substantially in many virological and probably some clinical parameters. The aim of this study was to analyze full-length HBV genome sequences from individuals with symptomatic acute HBV infections using phylogenetic and coalescent methods. The phylogenetic analysis resulted in the following subgenotype distribution: F1b (52.7%), A2 (18.2%), F4 (18.2%) and A1, B2, D3 and F2a 1.8% each. These results contrast with those previously reported from chronic infections, where subgenotypes F1b, F4, A2 and genotype D were evenly distributed. This differential distribution might be related to recent internal migrations and\/or intrinsic biological features of each viral genotype that could impact on the probability of transmission. The coalescence analysis showed that after a diversification process started in the 80s, the current sequences of subgenotype F1b were grouped in at least four highly supported lineages, whereas subgenotype F4 revealed a more limited diversification pattern with most lineages without offspring in the present. In addition, the genetic characterization of the studied sequences showed that only two of them presented mutations of clinical relevance at S codifyng region and none at the polymerase catalytic domains. Finally, since the acute infections could be an expression of the genotypes currently being transmitted to new hosts, the predominance of subgenotype F1b might have epidemiological, as well as, clinical relevance due to its potential adverse disease outcome among the chronic cases.","palabras_clave":"HEPATITIS B VIRUS|ACUTE INFECTION|GENOTYPES|EVOLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6513,"idioma_id":4,"titulo":"Genetic diversity, ancestry relationships and consensus among phenotype and genotype in banana (Musa acuminata) clones from Formosa (Argentina) farmers","resumen":"Though banana is a tropical crop, the Argentinean Northeastern Province of Formosa has extensively promoted its production. Genetic variation was assessed by the Amplified Fragment Length Polymorphism (AFLP) in a set of allotriploid and autotriploid clones from Formosa farms. Clones were also evaluated for perimeter and height of the pseudo-stem, number of leaves before flowering, number of flower clusters and length of fruits. Four international autotriploid varieties were used as samples. An AMOVA test widely discriminated among allotriploid clones, autotriploid clones and check varieties. The ancestry relationships between the autotriploids genotypes verified that clones were derived from the check varieties. Principal Coordinates Analysis was applied to assess the genetic diversity, demonstrating that 45% of the total molecular variation was explained by the three first principal coordinates. The first two principal components explained 77% of total phenotypic variability according to a Principal Components Analysis with the mean phenotypic values of clones and check varieties. Procrustes Analysis verified a high consensus (71.3%) among phenotypic and genotypic characterizations, suggesting that putative associations could be found among both sets of data. The 79.6% of the total variation was explained by the two principal components in this Procrustes Analysis.","palabras_clave":"MUSA|PROCRUSTES|ANÁLISIS MULTIVARIADO|AFLP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6514,"idioma_id":5,"titulo":"MACROHONGOS PRESENTES EN UN ÁREA DE MANEJO REGENERATIVO DE BOSQUE DE MATA ATLÁNTICA (MISIONES, ARGENTINA)","resumen":"El Bosque de Mata Atlántica es un ecosistema que histórica y continuamente se ve sometido a manejo forestal desmedido. Esto ha generado una fragmentación del ambiente boscoso con su consecuente pérdida de hábitat para todo tipo de organismos de la región. El presente trabajo es un catálogo de especies de macrohongos presentes en la zona de Oberá (Misiones, Argentina), donde desde 2009 se llevan a cabo tareas de recuperación y regeneración de biodiversidad del Bosque de Mata Atlántica. Se realizaron muestreos a campo en diferentes estaciones durante 3 años. Se identificaron 136 especies, 90 generos, 31 familias y 13 órdenes. El objetivo del presente trabajo fue generar un listado de especies de macrohongos presentes en la zona. Esto permitirá profundizar en distintos grupos de macrohongos y analizar su capacidad como bioindicadores del estado de conservación de la zona de Bosque Atlántico en proceso de recuperación.","palabras_clave":"Biodiversidad|Selva Paranaense|Macrohongos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6515,"idioma_id":4,"titulo":"Melatonin prevents\/restores the inhibition of the intestinal Ca2+ absorption caused by 1 glutathione depleting drugs","resumen":"We have previously demonstrated that melatonin (MEL) blocks the inhibition of the intestinal Ca2+ absorption caused by menadione (MEN). The purpose of this study were to determine whether MEL not only restores but also prevents the intestinal Ca2+ absorption inhibited either by MEN or BSO, two drugs that deplete glutathione (GSH) in different ways, and to analyze the mechanisms by which MEN and MEL alter the movement of Ca2+ across the duodenum. To know this, chicks were divided into four groups: 1) controls, 2) MEN treated, 3) MEL treated, and 4) treated sequentially with MEN and MEL or with MEN and MEL at the same time. In a set of experiments, chicks treated with BSO or sequentially with BSO and MEL or with BSO and MEL at the same time were used. MEL not only restored but also prevented the inhibition of the chick intestinal Ca2+ absorption produced by either MEN or BSO. MEN altered the protein expression of molecules involved in the transcellular as well as in the paracellular pathway of the intestinal Ca2+ absorption.  MEL restored partially both pathways through normalization of the &#729;O2&#8722; levels. The nitrergic system was not altered by any treatment. In conclusion, MEL prevents or restores the inhibition of the intestinal Ca2+ absorption caused by different GSH depleting drugs. It might become one drug for the treatment of intestinal Ca2+ absorption under oxidant conditions having the advantage of low or null side effects.","palabras_clave":"MELATONIN|MENADIONE|OXIDATIVE STRESS|PARACELLULAR CALCIUM TRANSPORT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6516,"idioma_id":4,"titulo":"Alterations of Endogenous Hormonal Levels in Plants under Drought and Salinity","resumen":"The phytohormones are pivotal chemical messengers produced within the plant that regulate its growth and development, and responses to environmental stimuli. Drought and salinity are adverse environmental factors that disturb the plant hormonal balance. Accordingly, these hormonal fluctuations modify the cellular dynamic and hence they play a central role in regulating plant growth responses to abiotic stresses such as drought and salinity. The present review gives an update about the alterations of endogenous phytohormones such as abscisic acid (ABA), auxins (Aux), cytokinins (CKs), ethylene (ET), gibberellins (GAs), jasmonates (JAs), salicylic acid (SA), brassinosteroids (BRs), strigolactones (SLs) and nitric oxide (NO) that occur as part of the adaptative responses of plant against drought and salt stresses. Better understanding of the endogenous hormonal changes during the plant response to both abiotic stresses will contribute, in part, to the development of stress-tolerant plants.","palabras_clave":"Drought|Plant Growth Responses|Phytohormones|Salinity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6517,"idioma_id":5,"titulo":"Utilización de imágenes satelitales para comprender la dinámica lagunar en la Región Pampeana","resumen":"El objetivo de este trabajo fue analizar imágenes satelitales de distintas resoluciones espaciales para interpretar el comportamiento morfométrico de seis lagunas de la Región Pampeana, Argentina, caracterizadas por tener distintos regímenes pluviométricos. Se analizó la respuesta morfométrica de cada una considerando su localización, condiciones de sitios y la ocurrencia eventos extremos secos y húmedos. Se utilizó el Índice Estandarizado de Precipitación (IEP) para la determinación de años húmedos, secos y normales. Este análisis mostró que las lagunas pampeanas no se comportan de la misma manera ante los eventos pluviométricos. Su origen, utilización socio-económica y régimen pluviométrico condicionan su variación espacio-temporal y morfométrica.","palabras_clave":"REGION PAMPEANA|LAGUNAS|PRECIPITACION|RESPUESTA ESPECTRAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6518,"idioma_id":5,"titulo":"Estudio preliminar del ajuste entre datos meteorológicos in situ y del Reanálisis (NCEP\/NCAR) en distintos ambientes de la provincia de Buenos Aires, Argentina","resumen":"El objetivo de este trabajo fue determinar de forma preliminar el ajuste que los datos del Reanálisis (NCEP\/NCAR) presentan en diferentes ambientes en su mínima escala espacial con mediciones in situ. El área de estudio es el sudoeste de la provincia de Buenos Aires (Argentina). Se seleccionaron 5 ambientes: continental, serrano, costero, urbano y estuarial. Se compararon los datos de temperatura del aire, humedad relativa y precipitaciones de ambas fuentes de información para el período 2010-2012. Se obtuvo que la temperatura del aire manifestó un buen ajuste en todos los ambientes, la humedad relativa solo en el ambiente urbano y la precipitación no presento ajuste en ninguno de ellos.","palabras_clave":"Reanálisis|Ambientes|Datos meteorológicos|SW de la provincia de Buenos Aires"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6519,"idioma_id":5,"titulo":"Downscaling de variables climáticas a partir del reanálisis NCEP\/NCAR en el sudoeste de la provincia de Buenos Aires (Argentina)","resumen":"The aim of this study is to propose an easy way to use the Reanalysis data, applying statistical downscaling, to estimate air temperature, relative humidity and precipitation in regions with a lack or shortage of meteorological data. The study region was the southwest of Buenos Aires province, Argentina. Statistical techniques were applied to in situ and Reanalysis (NCEP\/NCAR) numerical model data. In short periods (10 years), the best fits were observed in the air temperature, especially in autumn and spring, while relative humidity and precipitation presented some differences. However, in long periods (30 years) the estimated data is useful for climatic studies due to its good fit between in situ and Reanalysis model data.","palabras_clave":"DOWNSCALING|MODELOS|SERIES TEMPORALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6520,"idioma_id":5,"titulo":"Diversificación de la actividad turística de balnearios: el caso de Pehuen Co, Argentina","resumen":"Para desarrollar un Manejo Integral Costero (MIC) de localidades turísticas es necesario identificar posibles alternativas que ayuden a diversificar su turismo. La localidad de Pehuén Co (provincia de Buenos Aires, Argentina) es un típico caso de turismo sin diversificación. Se realizó un análisis de su oferta y demanda turística mediante encuestas y relevamientos. Esto permitió trazar una zonificación del área de estudio según el grado de desarrollo turístico. Como resultado se identifican nuevas zonas con potencial turístico que permitiría la diversificación de la economía local y regional. Estas alternativas potenciarían un uso sustentable de los recursos y atractivos turísticos de Pehuén Co.","palabras_clave":"TURISMO|BALNEARIOS|PEHUEN CO|DIVERSIFICACION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6521,"idioma_id":4,"titulo":"Screening and Evaluation of Variables for Determination of Sulfonylurea Herbicides in Water Samples by Capillary Zone Electrophoresis","resumen":"A simple and rapid capillary electrophoretic method was developed for the simultaneousdetermination of two sulfonylurea herbicides in water samples: metsulfuron-methyl (MSM) andnicosulfuron (NS). These herbicides are widely used in agricultural practices and their residuesrepresent potential environment pollutants in waters and soils. The effect of the pH of the backgroundelectrolyte solution (BGE), the percentage of methanol in the BGE and the separation voltage werestudied on the resolution of MSM and NS. A 23 factorial design was used to identify the main factorswhich significantly influenced the separation of these compounds. Other parameters were evaluatedby the univariate method. The experimental results show that using a BGE composed of 25 mmolL&#1048576;1sodium borate and 3% (v\/v) of methanol at pH 8.5 and applying 15 kV, satisfactory analysis time andresolution between peaks can be obtained. The linear range for both analytes was 0.1?2.0 gmL&#1048576;1.The detection limits were 0.034 gmL&#1048576;1 for MSM and 0.044 gmL&#1048576;1 for NS. Surface water andgroundwater samples were analyzed applying the new method, and the obtained results presentedsatisfactory recovery percentages (82%?102%).","palabras_clave":"CAPILLARY ELECTROPHORESIS|MULTIVARIATE OPTIMIZATION|SULFONYLUREA HERBICIDES|WATER SAMPLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6522,"idioma_id":5,"titulo":"VALIDACIÓN ARGENTINA DE LA SERIE 19 DEL SISTEMA INTERNACIONAL DE IMÁGENES AFECTIVAS (IAPS)","resumen":"Este trabajo tiene por objetivo validar la serie 19 del IAPS.","palabras_clave":"EMOCIONES|VALIDACION|IAPS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6523,"idioma_id":5,"titulo":"Ponderación de la información generada en la Estación Experimental Agropecuaria Oliveros del INTA (INTA EEA Oliveros) mediante el proceso analítico jerárquico","resumen":"En el siglo XXI, el subsistema de conocimiento científico-tecnológico se convirtió en el principal componente&nbsp;del capital cultural de un país viabilizando el desarrollo socioeconómico y la potencialidad de los países desarrollados. El Instituto Nacional de Tecnología Agropecuaria (INTA) es un organismo de ciencia y tecnología de Argentina entre cuyos objetivos está la generación y transferencia de conocimiento científico al sector agropecuario. Dicha información se genera en estaciones experimentales agropecuarias e institutos&nbsp;de investigación que lo integran y que abordan los problemas del sector en distintas regiones el país. Los&nbsp;sectores destinados a la investigación, experimentación y transferencia del conocimiento tienen como producto las publicaciones científicas, manuales, informes técnicos, cursos y talleres entre otros. Los objetivos&nbsp;de este estudio fueron: 1) clasificar la formas de presentación del conocimiento producido en la EEA Oliveros,&nbsp;así como seleccionar y definir los criterios y alternativas requeridos en la ponderación de este y 2) aplicar la&nbsp;ponderación a la producción de conocimientos de la EEA Oliveros para el año 2014. Para clasificar, agrupar y ponderar el producto de la investigación\/extensión generada por el INTA Oliveros se reunió un equipo de&nbsp;investigadores y extensionistas de la institución. El Proceso Analítico Jerárquico (AHP) fue utilizado para el&nbsp;presente estudio. Este permite, a partir de valoraciones preasignadas, priorizar un conjunto de elementos, según&nbsp;los juicios, y preferencias de los individuos del equipo, adoptando un valor consensuado. Los resultadosmostraron que los productos generados como información original (IO=revistas con y sin referato, así como&nbsp;presentación a congresos) representaron el 60.8% de la producción de conocimiento total; la información elaborada (IE=libros, capítulos libros, manuales y producción audiovisual) representó el 10.8% y la transferencia de conocimiento (TC=cursos, jornadas y disertaciones), el 28.4%. La metodología empleada resultó útil para ponderar la generación de conocimiento. Estos resultados pueden ser utilizados para comparar la producción de conocimiento entre períodos, entre unidades, consensuando los criterios con un equipo representativo de las estas, e incluso para ser utilizadas como insumo en análisis o evaluaciones de otras temáticas. De todas maneras, es aconsejable revisar y adecuar periódicamente los criterios y alternativas seleccionadas para acompañar los posibles cambios en enfoques y desarrollos que ocurren en investigación, según los períodos que se van atravesando.","palabras_clave":"ponderacion|publicaciones"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6524,"idioma_id":4,"titulo":"Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct","resumen":"Recent evidence demonstrated that most fertilizing mouse sperm undergo acrosomal exocytosis (AE) before binding to the zona pellucida of the eggs. However, the sites where fertilizing sperm could initiate AE and what stimuli trigger it remain unknown. Therefore, the aim of this study was to determine physiological sites of AE by using double transgenic mouse sperm, which carried EGFP in the acrosome and DsRed2 fluorescence in mitochondria. Using live imaging of sperm during in vitro fertilization of cumulus-oocyte complexes, it was observed that most sperm did not undergo AE. Thus, the occurrence of AE within the female reproductive tract was evaluated in the physiological context where this process occurs. Most sperm in the lower segments of the oviduct were acrosome-intact; however, a significant number of sperm that reached the upper isthmus had undergone AE. In the ampulla, only 5% of the sperm were acrosome-intact. These results support our previous observations that most of mouse sperm do not initiate AE close to or on the ZP, and further demonstrate that a significant proportion of sperm initiate AE in the upper segments of the oviductal isthmus.","palabras_clave":"Acrosome reaction|Sperm|Oviduct"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6525,"idioma_id":5,"titulo":"Brote de coccidiosis aguda en cabritos en el departamento Pilcaniyeu, provincia de Río Negro, Argentina","resumen":"Brote de coccidiosis aguda en cabritos en el departamento Pilcaniyeu, provincia de Río Negro, ArgentinaAgustín Martínez1*; Marcela Patricia Larroza1; Raúl Francisco Cabrera1; Marcos Subiabre2; Carlos Alejandro Robles11 Grupo de Salud Animal, Estación Experimental Agropecuaria - INTA Bariloche -Modesta Victoria 4450, Bariloche (8400) Río Negro, ArgentinaAgencia de Extensión Rural Bariloche. INTA BarilocheCorreo electrónico: martinez.agustin@inta.gob.ar ; robles.carlos@inta.gob.arRESUMENSe describe un brote de coccidiosis aguda en cabritos Angora en un establecimiento minifundista ubicado en el departamento de Pilcaniyeu, provincia de Río Negro, Argentina. En el hato compuesto por 14 madres y 17 crías, se produjo la muerte de tres cabritos de un mes de edad luego de 24-36 horas de iniciados los síntomas que consistieron en anorexia, debilidad general y postración. Se realizó la necropsia de un cuarto cabrito agónico con similar sintomatología detectándose la presencia de nódulos blanquecinos tapizando la mucosa del íleon y contenido hemorrágico en ciego. A fin de realizar un diagnóstico poblacional se tomaron muestras de materia fecal de los 13 cabritos asintomáticos restantes. Los hallazgos macroscópicos, microscópicos y parasitológicos determinaron que el brote fue debido a una infección mixta aguda por Eimeria arloingi y E. ninakohlyakimovae. La ausencia de sintomatología clásica de diarrea y el curso agudo caracterizaron al presente caso como un brote atípico.ABSTRACTOutbreak of acute coccidiosis in Angora goat kids from Pilcaniyeu, Rio Negro province, ArgentinaAn outbreak of acute coccidiosis in Angora goat kids in a small farm in Pilcaniyeu, Rio Negro Province in Argentina is described. In a flock of 14 adult goats and 17 kids, three one-month-old goat kids died within 24-36 hours after the onset of clinical signs, such as anorexia, weakness and recumbency. A fourth agonic goat kid with similar clinical signs was necropsied. Whitish nodular polyps in the ileum and hemorrhagic content in the cecum were found. Fecal samples from 13 asymptomatic kids were taken to reach a flock diagnosis. The macroscopic, microscopic and parasitological findings determined a mixed Eimeria arloingi and E. ninakohlyakimovae infection. This outbreak was considered atypical due to its acute course and the absence of diarrhea.","palabras_clave":"caprinos|coccidiosis|eimeria|patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6526,"idioma_id":4,"titulo":"DEFINITION PROCEDURE FOR STRATEGIC TECHNOLOGY RESOURCES IN SMALL MANUFACTURING FIRMS: A CASE STUDY","resumen":"En el contexto latinoamericano las pequeñas empresas manufactureras enfrentan fuertes limitaciones de competitividad, en virtud de un significativo rezago tecnológico en sus procesos de producción y deficiencias en las actividades degestión de sus tecnologías. Asimismo, existen marcadas dificultades en este segmento de empresas para el acceso a los diversos instrumentos estatales de promoción científica y tecnológica. Es por ello que resulta necesario disponer de una herramienta procedimental que favorezca la implementación de prácticas sistemáticas y sostenidas que posibiliten gestionar los recursos tecnológicos, en particular aquellos que resulten estratégicos para favorecer al mejoramiento del desempeño productivo de estos emprendimientos. Los objetivos del trabajo se centraron en el desarrollo de un procedimiento para determinar los recursos tecnológicos estratégicos, y su aplicación en una pequeña empresa de aserrío. Para atender los objetivos propuestos se realizó una investigación del tipo noexperimental, a través de un estudio descriptivo que incluyó un diagnóstico sectorial, la utilización de herramientas de análisis estratégico para evaluación de recursos tecnológicos tangibles e intangibles, el análisis de factores clave (internos) y de factores influyentes sectoriales (externos). Como resultado principal se destaca la construcción de un procedimiento metodológico que posibilita mejorar la utilización de los recursos tecnológicos existentes, a partir de un diagnóstico tecnológico inicial. La aplicación del procedimiento contribuyó a mejorar el proceso de toma de decisiones referidas a la gestión e innovación tecnológica en la pequeña empresa y su desempeño productivo.El desarrollo de la investigación coadyuvó también al cierre progresivo de la ?brecha? existente entre la teorización académica y el pragmatismo empresarial, posibilitó un aporte concreto al desarrollo empresarial local y regional. En función de lo realizado, se considera oportuno analizar en futuras investigaciones, los impactos que provoca la implementación del procedimiento en los indicadores de gestión tecnológica de las pequeñas empresas de este sector.","palabras_clave":"STRATEGIC MANAGEMENT|TECHNOLOGICAL RESOURCES|SMALL COMPANIES|MANUFACTURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6527,"idioma_id":5,"titulo":"Hacia la elaboración de criterios para la estimulación de la flexibilidad cognitiva mediante juegos digitales: aportes fundados en un estudio empírico","resumen":"Laestimulación de las funciones ejecutivas (FE) mediante juegos digitales es unapráctica que ha experimentado un importante crecimiento en diferentes ámbitos.En vista del objetivo que se persiga, resulta necesario contar con criteriosprecisos para la selección del software autilizar. El presente artículo se centra en un estudio correlacional entredistintas FE evaluadas transversalmente en una población infantil (N=40) delsegundo grado de la educación primaria de la ciudad de Rosario (Argentina) y sepropone articular los datos obtenidos con los modelos teóricos vigentes enbusca de criterios para la selección de videojuegos aplicables a laestimulación de la flexibilidad cognitiva. Se han hallado correlacionessignificativas ?aunque de moderado valor- entre esta función, la atención, lamemoria y el control inhibitorio; vínculos que fundamentan las pautas sugeridasen la discusión.","palabras_clave":"FLEXIBILIDAD COGNITIVA|ESTIMULACIÓN COGNITIVA|JUEGOS DIGITALES|VIDEOJUEGOS|FUNCIONES COGNITIVAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6528,"idioma_id":4,"titulo":"THERMO - LIGHTING OPTIMIZATION PROPOSAL FOR SCHOOL BUILDINGS IN SUBTROPICAL HOT ? HUMID CLIMATES: MONITORING AND COMPUTER SIMULATION ON AUTUMN PERIOD","resumen":"The rate of glazed area to floor area and to sun-exposed wall area of a room is one of the main relationshipsin energetically optimized architecture. This paper shows the results of the hygrothermal and lightingbehaviour on autumn period, of a Primary School which was monitored for a year. It was selected from auniverse of 60 school prototypes located in Resistencia, a hot-humid mid-latitude city in the Northeast of Argentina. The measurements were compared with simulations through Simedif, Ecotect and its Radiance interface, in real occupation conditions. A root mean square error of 0.6 &#9702;C was obtained. Then the thermal´behaviour of the building with an optimized envelope by regulating its glazed areas was simulated for the same period of autumn. Better hygrothermal comfort conditions were verified with the optimization proposal, as well as higher levels of illuminance and uniformity in the spatial distribution of natural light, which would be reflected in an electrical consumption saving for lighting. Consequently, this study will contribute to determining criteria for sizing suitably glazed areas to achieve wellness conditions, which are not covered in full in the current Planning and Building Codes, for school buildings in Resistencia.","palabras_clave":"SOLAR|ENERGY|PASSIVE|CONDITIONING|GLAZED|AREA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6529,"idioma_id":5,"titulo":"Lectura y aprendizaje a partir de las TIC en estudiantes de primer año universitario","resumen":"Una línea emergente de investigación desde la Psicología Educacional plantea la necesidad de estudiar laincorporación de las TIC en los escenarios educativos formales. En este marco, presentamos los resultados deun estudio descriptivo cuyo objetivo es conocer cómo están presentes las TIC en la lectura y en el aprendizajepor parte de estudiantes universitarios de primer año, desde sus propias perspectivas.Los resultados del estudio muestran, en lo referente a los aspectos condicionales de la lectura y el aprendizajepor medio de las TIC, que los estudiantes se centran principalmente en la utilización de estas tecnologíasen contextos académicos, con los propósitos de: leer, informarse, comunicarse, conocer, comprender algoque no entienden, buscar información o palabras, realizar y entregar trabajos, descargar materiales. Sobrelos procedimientos y procesos que llevan a cabo, en general no describen cómo leen y aprenden sino paraqué leen y aprenden mediante las TIC. Como resultados de sus lecturas y aprendizajes mediante las TIC,fundamentalmente expresan que las mismas les permiten adquirir, desarrollar, mejorar o construir contenidoo conocimientos. En general valoran positivamente a las TIC por su alta presencia cotidiana, comodidad,rapidez, practicidad y accesibilidad.","palabras_clave":"lectura|aprendizaje|tic"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6530,"idioma_id":5,"titulo":"Tareas de lectura y escritura y el interés por aprender en estudiantes secundarios del último año","resumen":"Este estudio, desarrollado desde la perspectiva socio cognitiva, se orientó a conocer las tareas de clase queeligen estudiantes secundarios según el interés por aprender que ellas les generan. El diseño de investigaciónes transeccional correlacional. Se implementó el Cuestionario L.E.A.C. (Solé, Miras, Gracia, et al. 2000) aestudiantes de nivel medio de cuatro escuelas de Río Cuarto, Córdoba, Argentina. Los resultados del análisisrealizado con estadística descriptiva y asociación entre variables muestran que usar internet, leer e identificarideas principales y leer y tomar notas son las tareas más elegidas. Más mujeres que hombres prefieren leer ytomar notas, y leer y elaborar un resumen por escrito. En tanto que, en las escuelas con orientación en gestiónde las organizaciones se eligen predominantemente: lectura por parte del docente de material escrito, leer yresponder preguntas, y leer y subrayar. Los resultados son discutidos desde la perspectiva conceptual de lastareas de lectura y escritura que implicarían decir el conocimiento y\/o transformarlo.","palabras_clave":"tareas|aprendizaje|interes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6531,"idioma_id":4,"titulo":"Comet assay application in evaluation the safe use of medicinal plants","resumen":"Genotoxicity of three aqueous extracts obtained of Acacia cavens(espinillo), Acacia furcatispina(garabato) and Prosopis torquata(tintitaco), was evaluated by comet assay. Three Concentrations (0.1, 10 and 20 mg\/mL to A. cavensand A. furcatispina; 0.2, 2 and 20 mg\/mL to P. torquata) were tested and the damage index for each was calculated. Based on the results, it was determined that both aqueous extracts of A. cavensand A. furcatispinashowed no damage DNA at 0.1 and 10 mg\/mL but, at 20 mg\/mL induced DNA damage (p< 0.05), showing moderate level damage (category 2). On the other hand, P. torquataextract was strongly genotoxic at all concentrations tested.","palabras_clave":"Acacia cavens|Acacia furcatispina|Genotoxicity|Comet Assay|Prosopis torquata"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6532,"idioma_id":5,"titulo":"¿Tiene la participación ciudadana todavía algún sentido? Lecciones de y para América Latina","resumen":"A casi 30 años del primer experimento de participación ciudadana institucional que se llevó a cabo en América Latina, pareciera que la pregunta que da título a estas notas se manifiesta de modo evidente. Parafraseando a Hannah Arendt en su cuestionamiento por sentido de la política frente al abismo de los totalitarismos que imperaron en el siglo XX, debemos preguntarnos cuál es la misión y el fin de la participación ciudadana en las democracias actuales y reales de nuestro tiempo en la región.","palabras_clave":"Participación Ciudadana|América Latina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6533,"idioma_id":4,"titulo":"Track analysis of Oribatid mites (Acari: Oribatida) of the Subantarctic subregion of South America","resumen":"We analysed distributional data of 30 species of Oribatid mites of the Subantarctic subregion of southern South Americain order to contribute to elucidate their biotic evolution. We constructed individual tracks for the species analysed, basedon published and unpublished records. After superposing them we obtained six generalized tracks and five nodes. Fourgeneralized tracks (T2, T3, T4 and T6) extend along and near the Andean ranges, whereas two generalized tracks (T1 andT5) may be artefacts caused by the lack of information. The generalized tracks and nodes show the complex relationshipsof the austral biota, as hypothesized in previous contributions based on other plant and animal taxa.","palabras_clave":"Nodes|Oribatid mites|Subantartic subregion|South America"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6534,"idioma_id":5,"titulo":"Las ganancias de la empatía. Una revisión del concepto de prosocialidad en la Vejez.","resumen":"El poseer vínculos de calidad con las personas que nos rodean ha estado siempre pensado como un factor central parauna vida óptima, en especial en la vejez. La posibilidad de crear y sostener relaciones interpersonales positivas conotros interviene de forma directa con la salud y las conductas prosociales se muestran como un medio eficaz para de este tipo de relaciones. Motivaciones prosociales tendentes hacia el voluntariado, conductas altruistas, el cuidado interpersonal, las conductas de ayuda, intimidad y confianza relacional y el perdón son ejemplos de ello. En este sentido, la empatía, es decir, el poder ponerse en el lugar del otro, tener en cuenta sus necesidades y perspectivas, hademostrado ser el prerrequisito indispensable para desarrollar este tipo de actitudes. Diversos estudios han mostrado las conductas prosociales muestran una influencia directa en la satisfacción con la vida, en especial en las personasde mayor edad. En el presente trabajo se relevan diferentes modelos teóricos e informes de investigación que abonan en este sentido y se proponen algunas posibles líneas futuras de investigación","palabras_clave":"empatía|prosocialidad|vejez"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6535,"idioma_id":4,"titulo":"New combinations in Tarenaya (Cleomaceae) for the Argentinian flora","resumen":"In the present work new combinations are made for three South American species originally described under Cleome: Tarenayacordobensis, T. lilloi and T. tucumanensis.","palabras_clave":"ARGENTINA|CLEOMACEAE|CLEOME|TARENAYA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6536,"idioma_id":4,"titulo":"Bidimensional modeling applied to oil extraction kinetics of microwave-pretreated canola seeds","resumen":"The effect of microwaves on canola oil extraction kinetics and on tocopherol and canolol content in oil was studied. Oil was extracted with hexane at constant temperature (298-333 K) at different times (300-64800 s). In general, for each temperature and time, a signi&#64257;cant yield increase was achieved by effect of the pretreatment. A modi&#64257;ed Fick´s diffusion model was proposed to explain the extraction process; the parameters were adjusted to obtain washing fractions and the effective diffusion coef&#64257;cient, which presented an Arrhenius-type temperature-dependence. The analysis of the parameters allowed to develop a modi&#64257;ed bidimensional diffusion model (temperature and time-dependent), appropriately correlating the data for pretreated and untreated samples. An increase in the pre-exponential parameter due to pretreatment was observed, evidencing the in&#64258;uence of microwave radiation on the sample structure. The pretreatment did not signi&#64257;cantly affect the oil quality indices, but increased the content of canolol and &#946; and &#948;-tocopherol isomers.","palabras_clave":"Canola oil extraction  |Microwave-pretreatment|Diffusion| coef&#64257;cient Arrhenius |Bidimensional model"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6537,"idioma_id":4,"titulo":"Humoral and cellular immune responses induced by the urease-derived peptide Jaburetox in the model organism Rhodnius prolixus","resumen":"AbstractBackground: Although the entomotoxicity of plant ureases has been reported almost 20 years ago, theirinsecticidal mechanism of action is still not well understood. Jaburetox is a recombinant peptide derived fromone of the isoforms of Canavalia ensiformis (Jack Bean) urease that presents biotechnological interest since itis toxic to insects of different orders. Previous studies of our group using the Chagas disease vector andmodel insect Rhodnius prolixus showed that the treatment with Jack Bean Urease (JBU) led to hemocyteaggregation and hemolymph darkening, among other effects. In this work, we employed cell biology andbiochemical approaches to investigate whether Jaburetox would induce not only cellular but also humoralimmune responses in this species.Results: The findings indicated that nanomolar doses of Jaburetox triggered cation-dependent, in vitro aggregationof hemocytes of fifth-instar nymphs and adults. The use of specific eicosanoid synthesis inhibitors revealed that thecellular immune response required cyclooxygenase products since indomethacin prevented the Jaburetox-dependentaggregation whereas baicalein and esculetin (inhibitors of the lipoxygenases pathway) did not. Cultured hemocytesincubated with Jaburetox for 24 h showed cytoskeleton disorganization, chromatin condensation and were positivefor activated caspase 3, an apoptosis marker, although their phagocytic activity remained unchanged. Finally, in vivotreatments by injection of Jaburetox induced both a cellular response, as observed by hemocyte aggregation, anda humoral response, as seen by the increase of spontaneous phenoloxidase activity, a key enzyme involved inmelanization and defense. On the other hand, the humoral response elicited by Jaburetox injections did not lead toan increment of antibacterial or lysozyme activities. Jaburetox injections also impaired the clearance of the pathogenicbacteria Staphylococcus aureus from the hemolymph leading to increased mortality, indicating a possibleimmunosuppression induced by treatment with the peptide.Conclusions: In our experimental conditions and as part of its toxic action, Jaburetox activates some responsesof the immune system of R. prolixus both in vivo and in vitro, although this induction does not protect theinsects against posterior bacterial infections. Taken together, these findings contribute to the generalknowledge of insect immunity and shed light on Jaburetox?s mechanism of action.","palabras_clave":"Cellular immunity|Humoral immunity| Ureases|Jaburetox|Rhodnius prolixus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6538,"idioma_id":4,"titulo":"Azospirillum brasilense inoculation, auxin induction and culture medium composition modify the profile of antioxidant enzymes during in vitro rhizogenesis of pink lapacho","resumen":"Optimal in vitroplant growth can be stimulated by selecting specific nutritional andenvironmental conditions. However, the culture conditions, dissection, anddisinfection of plant material are stressful and may induce disruption of theplant physiological homeostasis. This can be modified by inoculation with rhizobacteriaas Azospirillum brasilense, by theculture medium type, and by auxin induction. Here, we performed rootingexperiments in two auxin-free culture media with ?pink lapacho? (Handroanthus impetiginosus) shootspreviously induced with 0, 10, 30, or 50 &#956;M indole butyric acid (IBA) for 3days and inoculated with A. brasilenseCd and Az39. Peroxidase (PO), phenylalanine ammonia-lyase (PAL), and polyphenoloxidase (PPO) activities were determined on days 0, 3, 6, 9, 12, and 15. Also, weeklyabsolute rooting percentage was evaluated. All enzymatic activities were higherin A. brasilense-inoculated shoots,linked to early and high rooting percentage. The culture medium type and IBAconcentration also affected enzymatic activities. The positivecorrelation between PO and PAL activities on day 9 and successful final in vitro rooting of H. impetiginosus allows using these activities as early markers ofrhizogenesis reducing the selection time of easy-to-root plants. Thechanges in enzymatic levels performed here are discussed on the basis of theirrole in rooting and in vitro stress andcontribute to the knowledge of the physiology of trees and their interactionwith rhizobacteria.","palabras_clave":"HANDROANTHUS IMPETIGINOSUS|IN VITRO ROOTING |PEROXIDASE|PHENYLALANINE AMMONIA-LYASE|POLYPHENOL OXIDASE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6539,"idioma_id":5,"titulo":"Composición del ensamble de insectos del dosel de bosques subtropicales secos del Chaco Semiárido, Argentina","resumen":"El dosel arbóreo constituye un complejo hábitat con gran diversidad de insectos. Los estudios sobre insectos del dosel están mayormente concentrados en bosques húmedos tropicales y son escasos en bosques secos subtropicales. El propósito de este trabajo fue caracterizar la entomofauna del dosel de bosques secos subtropicales, analizando su diversidad regional y localmente. Para ello se muestrearon cinco sitios localizados en la región fitogeográfica del Chaco Semiárido Argentino. Las recolecciones de insectos se realizaron durante el período estival y mediante la técnica de vareo de ramas (beating). Se recolectaron 963 insectos pertenecientes a nueve órdenes y 79 familias. La composición taxonómica y la distribución de la abundancia de familias fueron diferentes en los sitios estudiados. Las condiciones ambientales propias y los disturbios antrópicos de los sitios influyeron en la determinación de los agrupamientos taxonómicos y en la distribución de la abundancia de los insectos. Los resultados que se presentan en este trabajo constituyen los primeros datos sobre la diversidad de insectos del dosel en bosques secos subtropicales, y aportan conocimientos iniciales para futuros estudios sobre interacciones ecológicas y, en general, sobre la diversidad del dosel de estos bosques.","palabras_clave":"ensamble de insectos|diversidad|Chaco Semiárido|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6540,"idioma_id":5,"titulo":"Desarrollo del aparato digestivo en pollos Campero INTA alimentados con poroto mucuna (Stizolobium deeringianum)","resumen":"Las leguminosas tropicales se utilizan en los sistemasde producción alternativos para la alimentación de aves. En el presente trabajo se evaluóel efecto de la incorporación de harina de poroto mucuna (Stizolobium deeringianum) en elalimento terminador, sobre el desarrollo del aparato digestivo en pollos Campero INTA. Ladieta control se elaboró en base a maíz, soja y una premezcla de vitaminas y minerales; enla dieta de prueba el poroto mucuna reemplazó parcialmente a la harina de soja. El análisiscomparativo se realizó mediante ANOVA en un arreglo factorial, operando el sexo y tipode alimento como efectos principales. No se constataron interacciones significativas entrelos distintos niveles de ambos factores para las variables dependientes analizadas. Se constatarondiferencias significativas para peso relativo del tracto gastrointestinal con valoresde 6,62±0,62 y 7,33±0,59% para las dietas control y mucuna respectivamente. El peso relativodel estómago muscular registró diferencias estadísticas significativas con valores de2,56±0,45 y 3,06±0,46% para las dietas control y mucuna respectivamente. Se demostraroncorrelaciones positivas y significativas entre el peso relativo del tracto gastrointestinal y delestómago glandular (r=0,82; p=0,01) e intestino delgado (r=0,72; p=0,04) en el grupo control.En este grupo experimental el estómago glandular correlacionó en forma positiva y significativa con el peso del intestino delgado (r=0,93; p=0,0007). En el grupo alimentado con mucuna se registró una correlación positiva y significativa entre el peso relativo del tracto gastrointestinal y del estómago muscular (r=0,79; p=0,02). Se concluye que la incorporación de poroto mucuna produce un incremento en el peso relativo del tracto gastrointestinal. Teniendo en cuenta que dicho poroto podría emplearse en pollos como fuente alternativa de  proteínas, se considera necesario profundizar los estudios sobre los métodos de eliminación de sus factores antinutricionales, para una más segura utilización práctica.","palabras_clave":"AVICULTURA|ALIMENTACIÓN|DIETAS|EFICIENCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6541,"idioma_id":5,"titulo":"ANÁLISIS DE UN DISPOSITIVO DIDÁCTICO FORMULADO POR ESTUDIANTES DE PROFESORADO EN MATEMÁTICA EN UN CURSO DE FORMACIÓN BASADO EN EL PARADIGMA DE LA INVESTIGACIÓN","resumen":"En este trabajo reportamos resultados de una investigación exploratoria y descriptiva, con estudiantes de profesorado en matemática, que experimentaron un curso para su formación con el propósito de vincularlos con el paradigma de la investigación. Una de las fases del curso fue que los estudiantes de profesorado propongan dispositivos didácticos para enseñar matemática en la escuela secundaria, en correspondencia con el curso realizado. En este trabajo se analiza el diseño de un dispositivo. Los principales resultados indican que los estudiantes formulan una cuestión generatriz apropiada para realizar una enseñanza basada en el paradigma de la investigación, pero tienen dificultades para explorar y ampliar la pregunta, y la reducen en sus alcances y generatividad.","palabras_clave":"FORMACIÓN DOCENTE|MATEMÁTICA|ENSEÑANZA|TEORÍA ANTROPOLÓGICA DE LO DIDÁCTICO (TAD)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6542,"idioma_id":5,"titulo":"SECUENCIA PARA ENSEÑAR CONCEPTOS ACERCA DE LA LUZ DESDE EL ENFOQUE DE FEYNMAN PARA LA MECÁNICA CUÁNTICA EN LA ESCUELA SECUNDARIA: UN ANÁLISIS BASADO EN LA TEORÍA DE LOS CAMPOS CONCEPTUALES","resumen":"En este trabajo se presenta un conjunto de situaciones que componen una secuencia diseñada para enseñar conceptos relativos a la luz, que incluyen y enfatizan su aspecto cuántico, con el fin de promover su conceptualización en estudiantes del último año de la escuela secundaria. La descripción y el análisis didáctico de las situaciones se realizan utilizando la Teoría de los Campos Conceptuales de Vergnaud, anticipando los posibles invariantes operatorios que los estudiantes utilizarán al afrontar las situaciones. Las situaciones se presentan en un marco conceptual unificador basado en el enfoque de Caminos Múltiples de Feynman para la mecánica cuántica. Se parte de un conjunto de experiencias relativas a la luz (en su mayoría realizables en el aula de clases) para luego formular una explicación cuántica de las mismas. Este abordaje ofrece una vía posible para que los estudiantes de la escuela secundaria construyan el sentido de los conceptos cuánticos: caminos alternativos, camino de tiempo mínimo y distribución de probabilidad en la Experiencia de la Doble Rendija. Se realizan consideraciones didácticas basadas en la Teoría de los Campos Conceptuales.","palabras_clave":"Didactic analysis|Light|Quantum|Feynman?multiple paths"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6543,"idioma_id":5,"titulo":"PATOLOGÍAS PODALES EN GANADO LECHERO Y EN FEEDLOT DEL PARTIDO DE TANDIL, PROVINCIA DE BUENOS AIRES, ARGENTINA","resumen":"Las patologías podales del bovino son multicausales; entre ellas se cuentan altos nivelesde producción y estrés, así como etiologías ambientales, nutricionales, infecciosas, genéticas,razas, estado fisiológico y comportamiento, tanto animal como humano. Estánadquiriendo gran importancia debido a la intensificación de la producción. El objetivo delpresente trabajo fue determinar la prevalencia de enfermedades podales en explotacioneslecheras y en feedlot, así como comparar ambos sistemas de explotación en la región deTandil, Buenos Aires, Argentina. A tal fin, se evaluaron 24 tambos en 2005 y un feedloten 2013 en busca de patologías podales. Los animales que presentaron claudicación sesepararon del rodeo y se examinaron clínicamente. Se calculó la prevalencia bruta y porpatologías de mayor presentación en ambos tipos de explotación y, además, la proporciónsegún miembro afectado. Se estimaron diferencias entre las prevalencias de cada tipo deproducción por medio de Chi cuadrado (p < 0,05) y Razón de Prevalencia. La prevalenciaen los 12.132 animales lecheros fue de 2,71% y en los 14.500 de feedlot de 0,57%(Razón de Prevalencia = 4,73; IC 95% = 3,72 a 6,02). La prevalencia de traumatismosen feedlot fue del 0,17% y en los tambos de 0,016% (p < 0,05); la de dermatitis digitalfue de 0,69% en los tambos y en feedlot de 0,13% (p < 0,05), mientras la prevalenciade dermatitis interdigital alcanzó 0,81% en los tambos y 0,1% en feedlot (p < 0,05).En ambos sistemas de producción, los miembros más afectados fueron los posteriores(90,4% feedlot y 88,1% tambos). La menor prevalencia de patologías podales en feedlotpodría deberse a la baja edad de los animales. No obstante, en los animales de feedlot hayuna significativa mayor prevalencia en cuanto traumatismos que en los tambos, mientrasque en estos se presentan más casos de dermatitis digital e interdigital.","palabras_clave":"ENFERMEDADES PODALES|BOVINOS|SISTEMAS DE PRODUCCIÓN|PREVALENCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6544,"idioma_id":4,"titulo":"Enhanced electrochemical detection of quercetin by Natural Deep Eutectic Solvents","resumen":"New trends in analytical chemistry encourage the development of smart techniques and methods aligned with Green Chemistry. In this sense, Natural Deep Eutectic Solvents represents an excellent opportunity as a new generation of green solvents. In this work a new application for them has been proposed and demonstrated. These solvents were synthesized by combinations of inexpensive and natural components like, Glucose, Fructose, Citric acid and Lactic acid. The different natural solvents were easily prepared and added to buffer solution in different concentrations, allowing the enhancement of electrochemical detection of an important representative antioxidant like quercetin (QR) with improved signal up to 380%. QR is a ubiquitous flavonoid widespread in plants and food of plant origin. The proposed method using phosphate buffer with a eutectic mixture of Citric acid, Glucose and water in combination with carbon screen printed electrodes exhibited a good analytical performance. Detection and quantification limits were of 7.97 and 26.3 nM respectively; and repeatability with %RSDs of 1.41 and 7.49 for peak potential and intensity respectively. In addition, it has proved to be faster, greener and cheaper than other sensors and chromatographic methods available with the additional advantage of being completely portable. Furthermore, the obtained results demonstrated that the proposed method is able for the determination of QR in complex food samples.","palabras_clave":"QUERCETIN|NADES|ELECTROCHEMICAL DETECTION|POLYPHENOLS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6545,"idioma_id":4,"titulo":"Catabolic response and phospholipid fatty acid profiles as microbial tools to assess soil functioning","resumen":"Microbial properties may help to provide an integrated view of changes in soil functioning associatedwith soil management or soil status. The fatty acid profiles of membrane phospholipids (PLFA) cangive the composition of ecophysiological groups of soil microbial communities, while catabolicresponse profiles (CRP) estimate the heterotrophic functional diversity in soils, both relevant to theunderstanding of the role of micro-organisms in the functioning of the soil. The objectives of thisstudy were (i) to evaluate the CRP and PLFA as microbial tools to characterize changes in soilfunctioning and (ii) clarify the relation among these microbial measurements, with other physical,chemical and biochemical soil properties. We compare the same soil subjected to differentmanagements and degrees of erosion. An undisturbed soil (UN), an old pasture soil (OP) and soilsunder continuous cultivation (NT) with four different depth of A horizon: 25 cm (NT 25), 23 cm (NT23), 19 cm (NT 19) and 14 cm (NT 14) were tested. Substrate-induced respiration of most substratesdiminished when cropping pressure increased (UN &gt; OP &gt; NT), and soil catabolic evenness, as adiversity index, decreased by increasing production pressure and soil erosion. The correlation foundamong most of the measured physical, chemical and biochemical soil properties with the catabolicevenness showed the potential of this measurement to provide an integrated view of soil functioning.The PLFA analysis showed that the composition of microbial community denoting a partial recoveryafter 10 yr under grazed grassland. The stress indicators showed that farming practices increasedmicrobial stress with the highest values found in the most eroded soils.","palabras_clave":"soil biodiversity|soil microbial indicators|soil status monitoring"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6546,"idioma_id":5,"titulo":"Confección de tuberculosis y paratuberculosis en un toro de la Provincia del Chaco, Argentina.","resumen":"Se describe la presentación de un caso de confección de tuberculosis y paratuberculosis en un toro Brangus de 8 años perteneciente a un rodeo de cría Basail (Chaco, Argentina). El animal presentaba síntomas típicos de paratuberculosis-diarrea, emaciación, deshidratación crónica- y fue diagnosticado utilizando la intradermoreacción con PPD bovino para tuberculosis, ELISA indirecto en suero para tuberculosis, así como necropsia e histopatología de órganos internos. El test ELISA resultó positivo y la tuberculinización negativa, sin embargo. el análisis histopatológico de muestras de pulmón e hígado permitió confirmar la presencia de lesiones compatibles con tuberculosis, mientras que las muestras de intestino y ganglios linfáticos mesentéricos revelaron evidencias de paratuberculosis.","palabras_clave":"Bovino|Tuberculosis|Paratuberculosis|Serología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6547,"idioma_id":5,"titulo":"El rol del arbolado urbano sobre la temperatura invernal de la villa balnearia de Pehuén Co (Argentina)","resumen":"El objetivo de este trabajo fue determinar el porcentaje ideal de cobertura vegetal para generar temperatura del aire más confortable durante los meses más fríos en la localidad costera de Pehuen Co (Argentina). Se subdividió su plano según la cantidad de vegetación: > al 80 %, entre 80 y 40 % y  80 %) se registró una mayor Ta en las horas de la mañana respecto de las otras dos clases. Se observó que donde las áreas arboladas fueron > 80 % la TST fuemenor y viceversa. Se concluyó que para generar los mayores beneficios en la implementación de actividades turísticas en los meses de invierno y\/o prevenir posibles situaciones desconfortables por frío la cobertura vegetal necesaria debe ser superior al 40 %. Estos resultados proporcionan una guía para la planificación urbana de localidades costeras turísticamente más sustentables.","palabras_clave":"VEGETACION URBANA|PEHUEN CO|NDVI|CLIMA URBANO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6548,"idioma_id":5,"titulo":"Docencia universitaria: aprendizaje organizacional y autonomía","resumen":"Este artículo expone los resultados de una investigación sobre la incidencia de la comunicación y las tecnologías en la gestión del conocimiento, el aprendizaje organizacional y la construcción de autonomía en una universidad pública argentina. Se caracterizan las organizaciones aprendientes y examinan las prácticas comunicativas y el aprendizaje organizacional en equipos de docencia, aportando un enfoque innovador al estudio de la gestión del conocimiento en lo que hace a la perspectiva comunicacional y su interpretación en el marco del proyecto de autonomía.  En el análisis del caso estudiado se advierte que aunque las estructuras universitarias evidencian condiciones adecuadas para la producción de conocimiento, su desarticulación con el proyecto estratégico universitario provoca pérdidas de información y conocimiento utilizables. Si bien en las prácticas docentes se perciben algunas tendencias innovadoras, los equipos no realizan  planificaciones sistemáticas para observar, revisar y modificar el comportamiento y aprender de los resultados obtenidos.","palabras_clave":"Comunicación|Innovaciones tecnológicas|Gestión del conocimiento|Transformaciones institucionales"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6549,"idioma_id":4,"titulo":"Bovine leukemia virus becomes established in dairy herds before the first lactation","resumen":"In this work, we studied seven groups of pregnant heifers from a consortium of dairy farms heavily infected with bovine leukemia virus (BLV). ELISA testing showed that the seroprevalence ranges of BLV in heifers between 36.1 and 66.5 %. No significant differences in proviral load were found when comparing heifers with adult cattle. Before their first delivery, more than 9.8 % of heifers show a high proviral load. Because BLV infection can occur during the first two years of life, the rationale of any strategy should be to take action as early as possible after birth.","palabras_clave":"HEIFERS|SEROPREVALENCE|BLV|PROVIRAL LOAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6550,"idioma_id":4,"titulo":"Validation of the Monte Carlo model for resuspension phenomena","resumen":"In this study we presen ta simulation model based on a Monte Carlo method to describe the resuspension of particles deposited on a flat surface due to air flow.Particles are attached to the surface through an adhesion force, and roughness effects between the particles and the surface are taken into account using a reduction factor.Two versions of the model are developed.In the first, the stochastic process used for particle resuspension is based on the evaluation of probabilities depending on the ratio between adhesion andaerodynamics forces and using a Metropolis function. In the second version,the resuspension probabilities are evaluated from a balance between the adhesion and the aerodynamics moments acting on each particle. A detailed comparison between the model results and different previous experiments is presented.Despite its simplicity,the model has a high capacity to describe the observed behaviour of the resuspended particle fraction as a function of the airvelocity.The good performance of the moment balance MC model version reveals the importance of considering the rolling mechanism in the resuspension phenomena modelling.","palabras_clave":"Resuspension|Monte Carlo simulation|Roughness|Air velocity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6551,"idioma_id":4,"titulo":"Orientation-Independent Empirical Mode Decomposition for Images Based on Unconstrained Optimization","resumen":"This paper introduces a 2D extension of the empirical mode decomposition (EMD), through a novel approach based on unconstrained optimization. EMD is a fully data-driven method that locally separates, in a completely data-driven and unsupervised manner, signals into fast and slow oscillations. The present proposal implements the method in a very simple and fast way, and it is compared with the state-of-the-art methods evidencing the advantages of being computationally efficient, orientation-independent, and leads to better performances for the decomposition of amplitude modulated?frequency modulated (AM-FM) images. The resulting genuine 2D method is successfully tested on artificial AM-FM images and its capabilities are illustrated on a biomedical example. The proposed framework leaves room for an nD extension (n > 2).","palabras_clave":"Empirical mode decomposition|unconstrained optimization|data-driven|non-stationary image"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6552,"idioma_id":5,"titulo":"La responsabilidad del arrendador derivada de daños ambientales indirectos ocasionados por el arrendatario en Argentina","resumen":"Las transformaciones productivas ocurridas en las últimas décadas en el sector agropecuario argentino han impulsado una división entre la titularidad de la tierra y su explotación. Este desdoblamiento es instrumentado habitualmente mediante contratos de arrendamiento en los que los dueños transfieren el uso y goce del recurso a nuevas formas de organizaciones empresariales (pools de siembra). En un contexto en el que comienzan a multiplicarse los conflictos ambientales asociados a explotaciones rurales insustentables, el presente trabajo lleva adelante una investigación que tiene por objetivo reinterpretar los derechos y obligaciones de las partes del contrato de arrendamiento, con un enfoque particular en los deberes ambientales del arrendador. Con base en el bloque normativo integrado por la Constitución Nacional, la Ley General del Ambiente y el reciente Código Civil y Comercial, concluimos que el arrendador está sometido a un verdadero deber de control sobre las actividades que se desarrollan sobre su propiedad, y que en caso de incumplimiento de este deber y frente a daños ambientales indirectos, podrá ser tenido por responsable y obligado a costear las reparaciones correspondientes.","palabras_clave":"CONTRATO DE ARRENDAMIENTO RURAL|RESPONSABILIDAD AMBIENTAL|DAÑOS AMBIENTALES|EXPLOTACION AGRICOLA|POOL DE SIEMBRA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6553,"idioma_id":5,"titulo":"Reinterpretando la Responsabilidad del Propietario ante Daño Ambiental Indirecto derivado de Actividades Riesgosas del Usufructuario y del Superficiario","resumen":"El Código Civil y Comercial de la Nación contiene diversos instrumentosjurídicos que permiten desdoblar la titularidad de un recurso productivo del derecho de uso y goce sobre el mismo, como los derechos reales de usufructo y superficie. Si al explotar el recurso el usufructuario o superficiario lleva a cabo actividades riesgosas que ocasionan daño ambiental indirecto, el sistema de responsabilidad civil se debe integrar con normativa de orden ambiental. En este esquema corresponde reinterpretar la norma aplicable y concluir que el propietario puede ser considerado responsable por tales daños cuando se apropie de parte de la mayor rentabilidad generada por dicha actividad","palabras_clave":"Responsabilidad Civil|Daño Ambiental |Actividades Riesgosas|Usufructo|Superficie"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6554,"idioma_id":4,"titulo":"Participation of the extrinsic cholinergic innervation in the action of nitric oxide on the ovarian steroidogenesis in the first proestrous in rats","resumen":"An ex-vivo Coeliac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-O) system and an ovary without peripheral neural influence from virgin rats in the first proestrous were used to test whether ovarian extrinsic innervation and nitric oxide (NO) affects steroidogenesis in the ovary. The CG and the ovary were placed in separate buffered-compartments, connected by the SON. Stimulation of the CG was achieved by 10(-6)M acetylcholine (Ach). The ovary without peripheral neural influence was placed alone in a buffered-compartment. To test a possible role of NO in the ovarian response to peripheral neural influence, 100&#956;M sodium nitroprusside (SNP, an NO donor) and 100&#956;M N(G)-nitro-l-arginine methyl ester (l-NAME, an inhibitor of NO synthase) were added to the ovarian compartment separately. In the CG-SON-O system, SNP into the ovarian compartment increased the concentration of NO, reduced the release of progesterone and increased the release of estradiol (E2), increasing the mRNAs related to their synthesis enzyme. The addition of l-NAME to the ovarian compartment caused an opposite effect. In the ovary alone, NO manifested an antisteroidogenic effect on both hormones. These results show that the ovarian extrinsic innervation maintains a direct relationship between NO and E2, both needed at high levels during the follicular phase, allowing the continuity of the estrous cycle.","palabras_clave":"FIRST PROESTROUS|OVARIAN EXTRINSIC INNERVATION|COELIAC GANGLION|NITRIC OXIDE|OVARIAN STEROIDOGENESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6555,"idioma_id":5,"titulo":"Producción de biomasa y respuesta a la fertilización de especies forrajeras subtropicales cultivadas en La Pampa deprimida bonaerense","resumen":"La producción estival de biomasa forrajera de la Pampa Deprimida Bonaerense resulta muchas veces deficitaria para la alimentación del ganado. Una alternativa para incrementarla es cultivar especies perennes de origen subtropical. Algunas de estas especies, además de producir cantidades importantes de biomasa, han mostrado buena adaptación a condiciones ambientales similares a las de esta área. A pesar de tratarse de especies perennes, los estudios realizados  hasta ahora en la zona generalmente abarcan sólo los dos primeros años de crecimiento del cultivo. Por lo tanto, los objetivos de este estudio fueron evaluar: i) la productividad de especies forrajeras perennes subtropicales en comparación con la del pastizal natural por un periodo de 5 años desde la implantación del cultivo y ii) evaluar el efecto de la fertilización nitrogenada sobre la producción de biomasa de las especies subtropicales en la zona. Las especies estudiadas fueron Panicum coloratum cv. Klein Verde; Chloris gayana cvs. Finecut y Topcut y Setaria sphacelata cv. Narok. El experimento se condujo en la localidad de Verónica (Punta Indio, Bs As), perteneciente a la región de la Pampa Deprimida Bonaerense. La producción de biomasa seca aérea (BSA) de las especies subtropicales superó la del pastizal natural, comparando igual periodo de crecimiento. La fertilización nitrogenada incrementó la producción de BSA. Hasta el tercer ciclo de crecimiento, hubo una buena recuperación de las plantas luego de los periodos invernales. Sin embargo, los cultivos de C. gayana y S. sphacelata no rebrotaron durante la primavera del cuarto ciclo de crecimiento. En cambio, P. coloratum continúa en producción luego de 5 años de la implantación del cultivo. Los datos obtenidos destacan que: i) las especies subtropicales incrementaron la productividad estival en relación al pastizal natural en la Pampa Deprimida Bonaerense, ii) la fertilización nitrogenada es una herramienta útil para incrementar la producción de biomasa y iii) es necesario incrementar el conocimiento sobre los factores que afectan la perennidad de las especies subtropicales en esta zona.","palabras_clave":"SUBTROPICALES|BIOMASA|FERTILIZACION|PAMPA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6556,"idioma_id":4,"titulo":"Xanthan pyruvilation is essential for the virulence of Xanthomonas campestris pv. campestris","resumen":"Xanthan, the main exopolysaccharide (EPS) synthesized by Xanthomonas spp., contributes to bacterial stress tolerance and enhances attachment to plant surfaces by helping in biofilm formation. Therefore, xanthan is essential for successful colo- nization and growth in planta and has also been proposed to be involved in the promotion of pathogenesis by calcium ion chelation and, hence, in the suppression of the plant defense responses in which this cation acts as a signal. The aim of this work was to study the relationship between xanthan structure and its role as a virulence factor. We analyzed four Xanthomonas campestris pv. campestris mutants that synthesize structural var- iants of xanthan. We found that the lack of acetyl groups that decorate the internal mannose residues, ketal-pyruvate groups, and external mannose residues affects bacterial adhesion and biofilm architecture. In addition, the mutants that synthesized EPS without pyruvilation or without the external mannose resi- dues did not develop disease symptoms in Arabidopsis thaliana. We also observed that the presence of the external mannose res- idues and, hence, pyruvilation is required for xanthan to suppress callose deposition as well as to interfere with stomatal defense. In conclusion, pyruvilation of xanthan seems to be essential for Xanthomonas campestris pv. campestris virulence.","palabras_clave":"Xanthomonas campestris|xanthan|biofilm|plant-pathogen interaction|Arabidopsis thaliana|stomatal aperture"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6557,"idioma_id":4,"titulo":"Development of an autochthonous starter culture for spreadable goat cheese","resumen":"The aim of this work was to select strains of LAB autochthonous from Santiago del Estero to formulate a starter culture for making spreadable goat cheese. Four strains were selected: CRL1799 (Lactobacillus fermentum with high acidifying activity), CRL1803 (Lactobacillus fermentum with high proteolytic activity), CRL1808 (Lactobacillus rhamnosus with production of exopolysaccharide) and CRL1785 (Enterococcus faecium with diacetyl-acetoin production). The selected strains showed qualities that make them useful as starter culture in the elaboration of spreadable cheese. This starter culture is an alternative that allows obtaining differentiated products. The inclusion of CRL1808 strains seems to improve the rheology and texture, excluding the use of additives.","palabras_clave":"lactic acid bacteria|autochthonous starter culture|spreadable goat cheese|biochemical activities"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6559,"idioma_id":4,"titulo":"'Small Heat Shock Proteins and the postharvest chilling tolerance of tomato fruit'","resumen":"Plants have the largest number of small heat shock proteins (sHsps) (15-42 kDa) amongeukaryotes, but little is known about their function in vivo. They accumulate in response todifferent stresses, and specific sHsps are also expressed during developmental processes such asseed development, germination, and ripening. The presence of organelle-specific sHsps appearsto be unique to plants. The sHsps expression is regulated by heat stress transcription factors(Hsfs). In this work, it was explored the role of sHsps in the chilling injury of tomato fruit. Thelevel of transcripts and proteins of cytoplasmic and organellar sHsps was monitored in fruitduring ripening and after cold storage (four weeks at 4 ºC). Expression of HsfA1, HsfA2, HsfA3and HsfB1 was also examined. Two cultivars of tomato (Solanum lycopersicum L.) contrastingin chilling tolerance were assayed: Micro-Tom (chilling-tolerant) and Minitomato (chilling sensitive).Results showed that sHsps were induced during ripening in fruit from both cultivars.However, sHsps were induced in Micro-Tom fruit but not in Minitomato fruit after storage at alow temperature. In particular, sHsp 17.4-CII and sHsp 23.8-M transcripts strongly accumulatedin Micro-Tom fruit and HsfA3 transcript diminished after cold storage. These data suggest thatsHsps may be involved in the protection mechanisms against chilling stress and substantiate thehypothesis that sHsps may participate in the mechanism of tomato genotype chilling tolerance.","palabras_clave":"sHsp|Hsf|POSTHARVEST|CHILLING INJURY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6561,"idioma_id":4,"titulo":"Evaluation of agreement between temporal series obtained  from electrocardiogram and pulse wave.","resumen":"Heart rate variability allows to study the cardiovascular autonomic nervous system modulation. Usually, this signal is obtained from the electrocardiogram (ECG). A simpler method for recording the pulse wave (PW) is by means of finger photoplethysmography (PPG), which also provides information about the duration of the cardiac cycle. In this study, the correlation and agreement between the time series of the intervals between heartbeats obtained from the ECG with those obtained from the PPG, were studied. Signals analyzed were obtained from young, healthy and resting subjects. For statistical analysis, the Pearson correlation coefficient and the Bland and Altman limits of agreement were used. Results show that the time series constructed from the PW would not replace the ones obtained from ECG.","palabras_clave":"VARIABILIDAD DE LA FRECUENCIA CARDÍACA|ONDA DE PULSO|VARIABILIDAD DE LA ONDA DE PULSO|VFC|VFC Y ONDA DE PULSO|SERIES TEMPORALES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6562,"idioma_id":4,"titulo":"Obtaining a dietary supplement from disposable cabbage leaves","resumen":"The present study aims at determining the most convenient operating conditions for obtaining a dietarycomplement of high quality, in terms of nutrient content and rehydration capacity, from disposableleaves of the white cabbage. Different thermal treatments were used for dehydration. Previously, somesamples were applied blanching and freezing. These technological treatments and pretreatments,applied at different stages of the productive process, were conducted in order to evaluate and comparetheir effect on the content of nutrients and substances to be preserved, which were present in the freshleaves. Then, the dietary complements obtained were analyzed. Proximal composition, vitamin Ccontent, phenols and antioxidant activity were determined. Results indicate that a microwave dryingprocess at 200 W following a 2 min blanching at 96±2 ºC resulted in an antioxidant dietary complementwith an energy value of 1415.76±25.84 kJ, showing interesting amounts of proteins, carbohydrates,crude fiber, lipids and ashes.","palabras_clave":"Cabbage|Dietary fiber|Antioxidant activity|Phenolic content|Drying, Microwave"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6563,"idioma_id":4,"titulo":"Localization and broadband follow-up of the gravitational-wave transient GW150914","resumen":"A gravitational-wave transient was identified in data recorded by the Advanced LIGO detectors on 2015 September 14. The event candidate, initially designated G184098 and later given the name GW150914, is described in detail elsewhere. By prior arrangement, preliminary estimates of the time, significance, and sky location of the event were shared with 63 teams of observers covering radio, optical, near-infrared, X-ray, and gamma-ray wavelengths with ground- and space-based facilities. In this Letter we describe the low-latency analysis of the gravitational wave data and present the sky localization of the first observed compact binary merger. We summarize the follow-up observations reported by 25 teams via private Gamma-ray Coordinates Network Circulars, giving an overview of the participating facilities, the gravitational wave sky localization coverage, the timeline and depth of the observations. As this event turned out to be a binary black hole merger, there is little expectation of a detectable electromagnetic signature. Nevertheless, this first broadband campaign to search for a counterpart of an Advanced LIGO source represents a milestone and highlights the broad capabilities of the transient astronomy community and the observing strategies that have been developed to pursue neutron star binary merger events. Detailed investigations of the electromagnetic data and results of the electromagnetic follow-up campaign will be disseminated in the papers of the individual teams.","palabras_clave":"ASTROPHYSICS|HIGH ENERGY|RELATIVITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6564,"idioma_id":5,"titulo":"CYNOPHALLA POLYANTHA (CAPPARACEAE), NUEVO REGISTRO PARA LA FLORA ARGENTINA","resumen":"En el presente trabajo se registra por primera vez para la flora argentina la presencia del pequeño árbol Cynophalla polyantha (Triana &amp; Planch.) Cornejo &amp; Iltis (Capparaceae), hallado recientemente en el norte de la provincia de Salta, en cercanías de la frontera con la República de Bolivia.","palabras_clave":"BOSQUES SECOS NEOTROPICALES|DISTRIBUCIÓN|DISYUNCIÓN|TAXONOMÍA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6565,"idioma_id":5,"titulo":"Variables intervinientes en el relevamiento físico de la infraestructura, mobiliario y servicios del sitio a escala local","resumen":"El verde urbano cumple una función simbólica dentro de la ciudad, y es representativo de la belleza, la sustentabilidad y la diversidad de la naturaleza (GEHL, 2014). Los espacios verdes públicos son un elemento de relevante contribución en la calidad de vida de las comunidades. Ellos ofrecen beneficios psicosociales, ambientales y económicos, fortaleciendo la identidad local y la valorización urbanística de un área.Por ello, la presencia de un espacio verde cuidado y equipado contribuye a la regeneración urbana y a la rehabilitación barrial, beneficio que se manifiesta en el incremento de los valores de propiedad, o del potencial turístico de un área. El diseño, calidad, integración al contexto existente, y puesta en valor como objeto patrimonial, fortalecen la amenidad visual  determinando su aptitud como atractor a escala local barrial (CABE, 2009). La existencia de instalaciones y equipamientos mejora la integración física y social entre los usuarios. Una mayor accesibilidad orientada a los jóvenes, los discapacitados y las personas de edad avanzada, aumenta su potencial como ámbito para el desarrollo de actividades culturales, sociales y comunitarias, tales como ferias, festivales, o  programas de salud. Este artículo resume las variables que caracterizan la condición física de un conjunto de plazas seleccionadas para el desarrollo del Proyecto de Investigación UBACyT 2014-2017, ?Evaluación de los servicios ecosistémicos de las áreas verdes urbanas y de su percepción por los usuarios en la Ciudad Autónoma de Buenos Aires?. Las variables elegidas se diferencian entre aquellas correspondientes al entorno inmediato de las plazas, y las que caracterizan a los propios espacios verdes a evaluar.","palabras_clave":"Espacios verdes públicos|Relevamiento físico|Evaluación"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6566,"idioma_id":4,"titulo":"GW150914: FIRST SEARCH FOR THE ELECTROMAGNETIC COUNTERPART OF A GRAVITATIONAL-WAVE EVENT BY THE TOROS COLLABORATION","resumen":"We present the results of the optical follow-up conducted by the TOROS collaboration of the first gravitational-wave event GW150914. We conducted unfiltered CCD observations (0.35?1 &#956;m) with the 1.5 m telescope at Bosque Alegre starting &#8764;2.5 days after the alarm. Given our limited field of view (&#8764;100 arcmin2), we targeted 14 nearby galaxies that were observable from the site and were located within the area of higher localization probability. We analyzed the observations using two independent implementations of difference-imaging algorithms, followed by a Random-Forest-based algorithm to discriminate between real and bogus transients. We did not find any bona fide transient event in the surveyed area down to a 5&#963; limiting magnitude of r = 21.7 mag (AB). Our result is consistent with the LIGO detection of a binary black hole merger, for which no electromagnetic counterparts are expected, and with the expected rates of other astrophysical transients.","palabras_clave":"ELECTROMAGNETIC|GRAVITATIONAL|OPTICAL|OBSERVATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6567,"idioma_id":4,"titulo":"Incidence rate of amyloidosis in patients from a medical care program in Buenos Aires, Argentina: a prospective cohort","resumen":"Background: There are limited data concerning the incidence density (ID) of ATTRwt, AL and AA amyloidosis in the Argentinean population. Our aim was to estimate the ID of ATTRwt, AL and AA amyloidosis at the Hospital Italiano Medical Care Program in Buenos Aires, Argentina.Methods: Population was all members of a hospital-based health maintenance organization who were affiliated since January 2006 to December 2014. Each person was followed contributing time at risk since January 2006 or enrollment date to the final date. Incident cases of amyloidosis were captured from the institutional registry of amyloidosis. Incidence rate was calculated with 95% confidence intervals.Results: During the nine-year study period, there were 15 patients with ATTRwt, 12 with AL and 2 with AA amyloidosis for 1 105 152 person-years of follow-up. The crude ID of ATTRwt amyloidosis was 13.5 (95%CI 8.1?22.4), that of AL amyloidosis 11 (95%CI: 6?19) and that of AA amyloidosis 1.8 (95%CI: 0.5?7.2) per 1 000 000 person-years. The highest ID was found in men (31.7 for ATTRwt, 15.9 for AL and 2.27 for AA amyloidosis per 1 000 000 person-years). The ID adjusted to the population of the city of Buenos Aires was 6.46 (95%CI: 3.17?9.74) for ATTRwt, 6.13 (95%CI: 2.57?9.7) for AL and 1.21 (95%CI: 0.56 to 2.99) for AA amyloidosis.Conclusions: This is the first paper to report the incidence density of ATTRwt, AL and AA amyloidosis in Latin America. Our results are consistent with other studies from other regions. Although systemic amyloidosis is a rare disease, it is a major health problem because of its morbi-mortality.","palabras_clave":"amiloidosis|epidemiologia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6568,"idioma_id":5,"titulo":"De waka a Virgen, de Virgen a waka: una reflexión sobre el estatuto de imágenes de culto coloniales desde la Antropología del Arte","resumen":"Este trabajo aborda el estatuto de ciertas imágenes de culto coloniales mediante una indagación de los alcances y límites de los lineamientos teóricos provenientes de la Antropología del Arte, en particular de dos autores que han tenido un fuerte impacto en este campo en las últimas décadas: Hans Belting y Alfred Gell. Específicamente abordamos el análisis de las propuestas desarrolladas por Belting en Imagen y culto y Antropología de la imagen, y por Gell en Art and Agency. Por un lado, examinamos los procesos de reconfiguración de la religiosidad andina en el pasaje del período prehispánico al colonial, a partir del culto a las wakas y su posible presencia en el de la Virgen María (más concretamente de la advocación de Pomata). Por otro, tomamos el problema de la atribución del carácter de ?persona? a las imágenes de culto, que resulta de determinadas formas de interacción  que se establecen entre ellas y los hombres (sacerdores, devotos, hacedores), lo que nos lleva a considerar las relaciones entre imagen, prototipo, mímesis y antropomorfismo.","palabras_clave":"ARTE COLONIAL|IDOLATRÍA|WAKAS|IMAGENES RELIGIOSAS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6569,"idioma_id":5,"titulo":"Las tesis y la producción de conocimiento en la educación universitaria de grado","resumen":"El presente trabajo pretende dar cuenta del proceso de elaboración de tesis, del desarrollo de la tutoría y de la relación tutor-tesista en la educación universitaria de grado, lo cual implica un acercamiento a la investigación en la Universidad, y cuya culminación ha de permitir el acceso al título universitario de grado. Esta temática se abordará en un entorno de ciencia posnormal, y como proceso de co-construcción de conocimiento. Se ha realizado una revisión bibliográfica y una reflexión sobre un modo deseado y un modo observado de tutoría, las cuales se complementan con una mirada de la realidad local desde el lugar de tutores con vasta experiencia. Se concluye sobre la necesidad de profesionalizar la tutoría y distinguir la función tutorial de la función docente.","palabras_clave":"TESIS DE GRADO|TUTOR Y TESISTA|TUTORÍA|CO-CONSTRUCCIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6570,"idioma_id":4,"titulo":"Theoretical and experimental study of isothiazolinone adsorption onto ordered mesoporous silica","resumen":"Mesoporous silica SBA-15 particles were synthesized in order to evaluate their effectiveness as encapsulating agents for a commercial biocide composed of a mixture of methylisothiazolinone and chloromethylisothiazolinone (MIT\/CMIT). Three powdered samples of silica particles having different textural properties, sizes and morphologies were hydrothermally obtained and then characterized by SEM, TEM, SAXS, and nitrogen adsorption?desorption measurements. Adsorption of the biocide on the prepared materials was investigated, and the results showed that adsorption capacities increase as the particle size becomes smaller. Higher biocide amounts were also retained on particles having increased pore size and pore volume. Additionally, a most probable interaction mechanism between MIT\/CMIT and SBA-15 is proposed on the basis of molecular modeling calculations. The theoretical approach indicates that two adsorption geometries with comparable minimum levels of strength can be adopted by the biocide: planar adsorption when the biocide molecule rings are adsorbed on the silica surface and vertical adsorption when the O atom of the MIT\/CMIT interacts with the H atom of silanols.","palabras_clave":"SBA-15 |Isothiazolinone |Adsorption"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6571,"idioma_id":5,"titulo":"Prospección con GPR para localizar una cisterna y una galeria en un sitio historico urbano","resumen":"En este trabajo se presentan las metodologías y los resultados de una prospección geofísica realizada con GPR en la Manzana de las Luces (Buenos Aires, Argentina), cuyo principal objetivo fue localizar con precisión una cisterna y una galería, construidas durante los siglos XVII-XVIII. Otro objetivo fue detectar estructuras de origen más reciente, tales como partes del piso y tuberías. Esta información resultará útil para la planificación de intervenciones arqueológicas en el lugar, y contribuirá a la preservación de dichas estructuras históricas, frente a obras arquitectónicas o de ingeniería civil que puedan realizarse en un futuro. Desde  el  punto  de  vista  geofísico,  el  sitio estudiado presenta  una  capa  de  suelo con contenidos de arcilla y humedad apreciables, lo que implicaba niveles medios a altos de atenuación de las ondas de GPR y, consecuentemente, limitada penetración. Asimismo, el sitio se encuentra en un ámbito urbano, con proliferación de objetos cercanos ?tanto en el suelo como en el aire- y fuentes variadas de radiofrecuencia, lo cual anticipaba la posibilidad de obtener secciones de datos complejas, con reflexiones numerosas y próximas entre sí, con formas e intensidades diversas. Estas características requerían alcanzar un buen nivel de resolución en las secciones de datos para que dichas reflexiones pudieran ser distinguidas y correctamente interpretadas. Para eso era necesario tener ciertos recaudos, especialmente al momento del diseño de la prospección y durante el análisis e interpretación de los datos.La realización de pruebas previas a la prospección definitiva permitió definir un conjunto de parámetros de medición que hizo posible detectar reflexiones con amplitudes intermedias y bajas, con buena definición de las mismas. Además, la representación simultánea de los datos en las formas de secciones verticales y plantas favoreció la detección de reflexiones que, en principio, eran detectables en sólo una de estas formas de representación. Las reflexiones detectadas mediante ambos tipos de gráficos fueron correlacionadas entre sí, clasificadas y finalmente interpretadas en términos de sus probables orígenes. La metodología empleada permitió determinar las ubicaciones de la cisterna y la galería, con una precisión de unos pocos centímetros. También se detectaron tuberías, así como diversas discontinuidades y una malla de refuerzo en la estructura del piso.Los objetos que fueron centro de esta investigación y el tipo de entorno descrito resultan comunes a distintos sitios arqueológicos de Argentina y el exterior. En este sentido, los procedimientos y resultados mostrados sirven como un ejemplo o caso de referencia al respecto. Un objetivo particular del trabajo es revelar la utilidad y las limitaciones de la metodología empleada, en relación con las condiciones que imponen el tipo de aplicación y entorno. Otros objetivos son ilustrar los distintos tipos de reflexiones que pueden obtenerse en estos casos, y mostrar un análisis pormenorizado de las mismas y sus interpretaciones en términos de los probables orígenes. Es de esperar que estos contenidos resulten de interés para aquellas personas que trabajan con GPR en esta clase de sitios, así como para colegas con un interés más general en estas temáticas.","palabras_clave":"PROSPECCION GEOFISICA|GEORADAR|SITIOS HISTORICOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6572,"idioma_id":5,"titulo":"Nitrógeno mineralizado en anaerobiosis: relación con sistemas de cultivo de agricultura continua","resumen":"El nitrógeno (N) mineralizado en incubación anaeróbica corta (NAN) podría ser utilizado como indicador de salud edáfica. Para ajustar su utilización, es necesario caracterizar y comprender cómo es afectado por distintas prácticas de manejo. Para suelos del Sudeste Bonaerense se hipotetiza que i) mayores aportes de carbono (C) por los residuos de los cultivos se asocian a menores variaciones de NAN en la capa arable, siendo similares para labranza convencional (LC) y siembra directa (SD), y ii) la cantidad y la calidad del residuo del cultivo antecesor afectan el NAN. Los objetivos fueron: i) evaluar la evolución del NAN en 0-20 cm bajo distintas secuencias de cultivos bajo LC y SD, y con y sin fertilización nitrogenada (FN) y, ii) analizar el efecto de distintos residuos sobre el NAN en 0-20 cm. Se determinó NAN en muestras de suelo almacenadas tomadas a 0-5 y 5-20 cm de dos ensayos de sistemas de labranza (SL) (LC y SD): Ensayo 1 con dos niveles de FN (Con y Sin N), y Ensayo 2, con tres secuencias de cultivos (maíz-maíz-trigo, maíz-soja-trigo, soja-soja-trigo). El NAN disminuyó con los años de agricultura en 0-5, 5-20 y 0-20 cm, bajo ambos SL y niveles de FN, con diferencias entre SL sólo en 0-5 cm (LC>SD). En 0-20 cm no hubo diferencias en las disminuciones de NAN ni entre niveles de FN ni entre secuencias de cultivos, es decir, diferentes aportes de C no provocaron variación diferencial de NAN. Distintos cultivos antecesores tampoco afectaron el contenido de NAN. La FN podría haber producido una mayor tasa de mineralización del C orgánico del suelo al disminuir la relación C\/N del sistema, enmascarando el efecto del aporte de C sobre el NAN. No obstante, los resultados contribuyen a confirmar la viabilidad del NAN como indicador de salud edáfica.","palabras_clave":"nitrógeno potencialmente mineralizable|secuencias de cultivos|sistemas de labranza|fertilización nitrogenada"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6574,"idioma_id":4,"titulo":"Argentinian phlebotomine fauna, new records of Phlebotominae (Diptera: Psychodidae) for the country and the province of Chaco","resumen":"Sand flies are insects of medical and veterinary importance, because some species are able to transmit several pathogens such as Bartonella spp., Phlebovirus spp., and protozoan parasites of the genus Leishmania (Ross). They are widelydistributed in the Americas, with recordings ranging from Canada to Argentina.With the new recordings presented in this paper, the total number of known species in the country has thus been expanded to 36, and the number of species reported in the Argentinian Chaco bioregion to 19. The Chaco has now therefore becomes the second province in terms of the number of species cited in the country, after the province of Misiones.","palabras_clave":"Lutzomyia|Leishmania|Vector"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6575,"idioma_id":4,"titulo":"Characterization of Feeding Injuries Caused by Ceresa nigripectus Remes Lenicov (Hemiptera: Membracidae) on Alfalfa Stems (Medicago sativa L., Fabaceae)","resumen":"Piercing-sucking insects cause mechanical and physiological injury to plants. Ceresa nigripectus is a pest of alfalfa (Medicago sativa L.) in subtropical regions of South America, and a carrier of the ArAWB phytoplasma. The aim of this study was to determine the feeding habits of this treehopper and describe the effects of the feeding injuries on vascular tissues of alfalfa. Adults and nymphs of C. nigripectus inserted their stylets repeatedly girdling the stem. One week after feeding, alfalfa stems exhibited numerous food canals with salivary deposits, most of which reached the phloem. Two weeks after feeding, cortex and phloem cells next to the salivary sheath collapsed, mature tracheary elements became sparse and appeared with an increased cross-section area, and phenolic compounds increased in cells and cell walls compared to undamaged plants. Three weeks after feeding, an annular callus, formed by abnormal cell division and hypertrophy of preexisting cortex and vascular cambium cells, appeared immediately above the stem girdle. Parenchyma cells from the outer layers of the callus differentiated to form secondary anomalous amphicribal bundles in the wound. The aerial parts above the stem girdle eventually withered and died.","palabras_clave":"Sap feeders|feeding damage|plant wound response"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6576,"idioma_id":4,"titulo":"Descriptions of the immature stages and new host plant records of Notozulia entreriana (Berg) (Hemiptera: Cercopidae) pests of grasses in subtropical areas of the Americas","resumen":"Notozulia entreriana (Berg) (Hemiptera: Cercopidae) is one of the most common spittlebugs inhabiting the subtropical region of the America, inflicting important economic damage to grass crops. The immature stages are described and illustrated; the main characteristics that distinguish instars are the body size, color, number of flagellomeres, and number of tibial and metatarsomere spines. A key for identification of nymphs is provided as a tool to develop field studies.  Nine host plants, all belonging to Poaceae, are recorded as breeding and feeding host plants from different localities in northern Argentina.","palabras_clave":"HEMIPTERA|SPLITTEBUGS|NYMPHS|KEY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6577,"idioma_id":4,"titulo":"Foliar anatomy of Viola maculata growing in Parque Nacional Los Alerces, Chubut, Patagonia, Argentina","resumen":"tViola maculata Cav., Violaceae, grows in the underwood along the Los Andes mountain system fromNeuquén to Tierra del Fuego and Islas Malvinas. It is also known as violeta amarilla or as pilundeu, and isused in popular medicine by the Mapuches as stimulant, in dermatology, and as a lavage in eye diseases.The aim of this work is to examine the morpho-anatomical traits of the leaves and petiole of V. maculata(collected at Parque Nacional Los Alerces, Patagonia, Argentina). Cross sections and scanning electronmicroscopy of leaves blade and petiole showed a simple organization with simple unicellular trichomesand cells containing tannins, and crystals of calcium oxalate mainly in the spongy parenchyma. As V.maculata is used in traditional medicine, the specification of the anatomical characters is relevant todescribe the species.","palabras_clave":"Viola maculata|Violeta amarilla|anatomía foliar|farmacobotánica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6578,"idioma_id":4,"titulo":"'DNA profile of dog feces as evidence to solve a homicide'","resumen":"Dog fecal samples were collected at the crime scene and from the shoes of the suspect to see whether they could be linked. DNA was genotyped using a 145 bp fragment containing a 60 bp hotspot region of the mitochondrial DNA (mtDNA) control region. Once the species origin was identified, sequences were aligned with the 23 canine haplotypes defined, showing that evidence and reference had 100% identity with haplotype 5. The frequency of haplotype 5 and the exclusion power of the reference population were 0.056 and 0.89, respectively. The forensic index showed that it was 20 times more likely that the evidence belonged to the reference dog than to some other unknown animal. The results support that the mtDNA hypervariable region 1 (HV1) is a good alternative for typing in trace or degraded casework samples when the STR panel fails, and demonstrate the utility of domestic animal samples to give additional information to solve human legal cases.","palabras_clave":"Forensic sciences|Non-human DNA|Dog|Mitochondrial DNA|Feces"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6579,"idioma_id":4,"titulo":"Intelligent Systems in Modeling Phase of Information Mining Development Process","resumen":"The Information Mining Engineering (IME) understands in processes, methodologies, tasks and techniques used to: organize, control and manage the task of finding knowledge patterns in information bases. A relevant task is selecting the data mining algorithms to use, which it is left to the expertise of the information mining engineer, developing it in a non-structured way. In this paper we propose an Information Mining Project Development Process Model (D-MoProPEI) which provides an integrated view in the selection of Information Mining Processes Based on Intelligent Systems (IMPbIS) within the Modeling Phase of the proposed Process Model through a Systematic Deriving Methodology.","palabras_clave":"Development Process|Modeling Phase|Information Mining|Intelligent Systems"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6580,"idioma_id":5,"titulo":"La expansión urbana y sus impactos sobre el clima y la sociedad de la ciudad de Bahía Blanca, Argentina","resumen":"El objetivo de este trabajo fue analizar los efectos de la expansión urbana de Bahía Blanca sobre el microclima y la biomasa considerando la percepción de sus habitantes. Se utilizaron imágenes satelitales del período 1986-2014. Se estimó el área urbana, la temperatura y la biomasa. Se analizaron datos meteorológicos de temperatura, humedad relativa y velocidades máximas de viento provistos por el Servicio Meteorológico Nacional (Argentina). El crecimiento de la ciudad redujo la biomasa. La TST disminuyó 0.6 ºC debido al  aumento de las sombras generadas por los edificios, a la densidad de los mismos y a la capacidad calorífica de los materiales que los componen, entre otros factores.  La temperatura del aire se incrementó 0.7 ºC y la humedad relativa disminuyó un 6 %. La opinión de los habitantes coincidió con los datos meteorológicos analizados. La consulta a los actores sociales generó información útil para orientar la planificación de la ciudad, las políticas públicas y la toma de decisiones para mejorar la calidad de vida de los habitantes.","palabras_clave":"EXPANSION URBANA|MICROCLIMA|BAHIA BLANCA|BIOMASA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6581,"idioma_id":5,"titulo":"ESTUDIO COMPARATIVO DE TRES MODELOS CLIMÁTICOS EN ARGENTINA","resumen":"En algunas regiones del planeta, la escasez de estaciones meteorológicas con largas series de datos, ocasiona problemas para la caracterización del clima. En este contexto, adquieren importancia los modelos numéricos de obtención de datos climáticos. Uno de los mayores problemas que se presentan en Argentina es la desigual distribución espacial de las estaciones meteorológicas con registros de 50 años, lo que dificulta el estudio de su clima. El objetivo de esta investigación fue establecer para Argentina el modelo numérico que mejor represente las variables de: precipitación, humedad relativa, temperatura media, máxima y mínima del aire. Para ello, se analizaron datos históricos in situ pertenecientes al Servicio Meteorológico Nacional para el período 1950-2010 y se consideraron los resultados obtenidos de los modelos Reanálisis (NCEP\/NCAR), WorldClim y el NEW01. Para su comparación se aplicaron diferentes técnicas estadísticas (análisis de varianza, test de diferencia mínima significativa, análisis de regresión, coeficientes de correlación, determinación y concordancia). Además, se utilizaron métodos de interpolación para la representación gráfica de los resultados mediante un SIG. Los modelos resultaron útiles para caracterizar variables climáticas en zonas carentes de registros. El WorldClim tuvo un mejor ajuste para los datos de temperatura media del aire, mientras que el NEW01 fue mejor para la caracterización de las precipitaciones.&nbsp;","palabras_clave":"MODELOS CLIMATICOS|ANALISIS ESTADISTICO|VARIABLES METEOROLOGICAS|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6582,"idioma_id":4,"titulo":"Chemical Composition and Physical Properties of High Oleic Safflower Oils (Carthamus tinctorius, Var. CW88-OL and CW99-OL)","resumen":"Chemical composition and physical properties of CW88-OL and CW99-OL cultivars of high oleic safflower seeds and their hexane-extracted oils were determined. Dry-based seed composition of CW88-OL and CW99-OL was: moisture=4.29 and 4.23 %, oil=42.29 and 46.44 %, Crude protein= 20.94 and 16.41 %, neutral detergent fiber= 28.11 and 28.49 %, ash= 1.55 and 2.01 %, phosphorus content= 2033 and 3995 mg\/kg, respectively. Major fatty acids in oils were ~78% oleic (O), ~13% linoleic (L), ~5% palmitic (P) and ~2% stearic (St) acids, for both cultivars. Main triacylglycerols were OOO (~50%), OOL (~20%), SOL+OPO (~10%), and LLP (~5%). The oil composition of CW88-OL and CW99-OL in main minor components was: &#945;-tocopherol= 582 and 551 mg\/kg, total sterols= 3996 and 3362 mg\/kg, phospholipids= 22.1 and 21.1 mg\/kg and wax content= 70 and 74 mg\/kg. For both cultivars, density and viscosity of the oils between 25 and 55 ºC varied from 903.4 to 912.6 kg\/m3 and 63 to 23 mPa.s showing linear and exponential behaviors, respectively. Refractive index was 1.4694. CIELab color parameters were: 89.69 and 89.53 (L*), -3.72 and -3.07 (a*), and 47.28 and 47.78 (b*) (CW88-OL and CW99-OL, respectively).Thus, the high oil content of the seeds and nutritional quality of the oil accompanied by low levels of waxes and phospholipids makes the cultivars studied promising for producers and consumers.","palabras_clave":"SAFFLOWER|OIL-SEEDS|CHEMICAL COMPOSITION|PHYSICOCHEMICAL PROPERTIES|TRIACYLGLYCEROLS|WAXES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6583,"idioma_id":4,"titulo":"Land-use change affects the functionality of soil microbial communities: A chronosequence approach in the Argentinian Yungas","resumen":"Land-use change has drastically reduced the area of pristine forests in tropical and subtropical regions. In NW Argentina, Yungas forests were among the most affected by deforestation for the implementation of agricultural crops. Conversion of forests to croplands modified the structure and function of soil microbial communities, but its effects on soil functionality across time after land-use change are understudied. Therefore, the objective of this study was to analyze the impact of land-use change and time under cultivation on the functionality of microbial communities in these soils. We established a 30-year old chronosequence comprising 4 stages (forest and short-, mid- and long-term agriculture) in 3 independent farms. Together with soil physicochemical properties, we measured microbial biomass carbon, basal respiration, ammonification, acid and alkaline phosphomonoesterase activities, and community-level physiological profiling (CLPP). During the first years of cultivation, the functionality and biomass of soil microbial communities were strongly affected. Compared to forest soils, short-term agricultural soils exhibited a reduction on microbial biomass (&#8764;45%), ammonification (&#8764;67%) and acid phosphomonoesterase activity (&#8764;41%). Moreover, increased basal respiration (up to 94%) and metabolic quotient in those soils suggested radical changes in functionality at the beginning of the chronosequence. However, CLPP evidenced that older agricultural sites had an increased global catabolic response per unit biomass, while it detected no differences in physiological diversity of soil microbial communities along the chronosequence. The lack of differences detected between mid- and long-term agricultural sites, in addition to a reduced inter-site variability, evidences an apparent stabilization and homogenization of soil microbial communities towards the end of the chronosequence. In contrast with physicochemical variables, microbiological variables had a greater performance for characterizing the different stages of the agricultural chronosequence and the impact of land-use change on soil functionality.","palabras_clave":"DEFORESTATION |SOYBEAN |MICROBIAL ACTIVITY|CLPP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6584,"idioma_id":5,"titulo":"EL RITMO DE LA APRECIACIÓN ESTÉTICA COMO VARIACIÓN ORDENADA DE LOS CAMBIOS EN EL ARTE COMO EXPERIENCIA DE JOHN DEWEY","resumen":"El ritmo de la apreciación estética comovariación ordenada de los cambios en el Artecomo experiencia de John DeweyHoracio Héctor Mercau*ResumenLas finalidades de las que Dewey nos habla a lo largo de toda su extensa obra puedenser analizadas desde tres perspectivas: la primera es el término de una transacción ofines cualitativos; la segunda, el fin-en-perspectiva que es imaginativamenteproyectado y deseado, y, tercero, la consumación de una experiencia controlada. Laintensión de este trabajo es mostrar que para Dewey esta culminación debería ser unaobra de arte que entra en la experiencia de aquellos que activamente la comparten ytoman parte de la misma. Los fines-en-perspectiva marcan una manera crítica de hacerfilosofía, los fines cualitativos se dan en toda transacción de la experiencia, y lasconsumaciones como obras de arte se desarrollan manifestando un ritmo ocontinuidad en la experiencia. Una continuidad o variación ordenada que no es otracosa que una inteligencia que predice lo que será deseable e indeseable enposibilidades futuras y que ingeniosamente idea medios para alcanzar un bienimaginado. Para llevar adelante descripción nos abocaremos a un análisis concienzudode la obra el Arte como experiencia en la cual sienta las bases para comprender que sunoción de percepción aporta las vías a través de las cuales el espíritu y la naturaleza secompenetran a través del cuerpo y el comportamiento para producir nuestra increíbleexperiencia.Palabras clave: Apreciación ? Experiencia ? RitmoAbstractThe aims of which Dewey speaks to us throughout his extensive work can be analyzedfrom three perspectives: the first is the completion of a transaction or qualitativepurposes; the second, the end-in-perspective that is imaginatively designed anddesired, and thirdly, the consummation of a controlled experience. The intent of thispaper is to show that for Dewey this culmination should be a work of art that comesinto the experience of those who actively share and take part of it. The late-inperspectivemarks a critical way of doing philosophy, qualitative purposes are given inany transaction of experience, and completions as works of art manifesting develop arhythm or continuity of experience. Orderly continuity or change that is nothing but anintelligence that predicts what is desirable and undesirable in future possibilities andmeans ingeniously idea to achieve a well imagined. To carry out Description we willfocus on a thorough analysis of the work of art as experience which lays the foundationfor understanding that his notion of perception provides the pathways through whichthe spirit and nature penetrate through the body and behavior to produce our amazingexperience.Keywords: Appreciation ? Experience ? Ritmo","palabras_clave":"Experiencia|Apreciación|Creación|Arte"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6585,"idioma_id":4,"titulo":"Novel insights of ethylene role in strawberry cell wall metabolism","resumen":"Due to its organoleptic and nutraceutical qualities, strawberry fruit (Fragaria x ananassa, Duch) is a worldwide important commodity. The role of ethylene in the regulation of strawberry cell wall metabolism was studied in fruit from Toyonoka cultivar harvested at white stage, when most changes associated with fruit ripening have begun. Fruit were treated with ethephon, an ethylene-releasing reagent, or with 1-methylcyclopropene (1- MCP), a competitive inhibitor of ethylene action, maintaining a set of non-treated fruit as controls for each condition. Ethephon treated-fruit showed higher contents of hemicelluloses, cellulose and neutral sugars regarding controls, while 1-MCP-treated fruit showed a lower amount of those fractions. On the other hand, ethephon-treated fruit presented a lower quantity of galacturonic acid from ionically and covalently bound pectins regarding controls, while 1-MCP-treated fruit showed higher contents of those components. We also explored the ethylene effect over the mRNA accumulation of genes related to pectins and hemicelluloses metabolism, and a relationship between gene expression patterns and cell wall polysaccharides contents was shown. Moreover, we detected that strawberry necrotrophic pathogens growth more easily on plates containing cell walls from ethephon-treated fruit regarding controls, while a lower growth rate was observed when cell walls from 1-MCP treated fruit were used as the only carbon source, suggesting an effect of ethylene on cell wall structure. Around 60% of strawberry cell wall is made up of pectins, which in turns is 70% made by homogalacturonans. Our findings support the idea of a central role for pectins on strawberry fruit softening and a participation of ethylene in the regulation of this process.","palabras_clave":"CELL WALL|MIDDLE LAMELLA|PECTINS|ETHYLENE|STRAWBERRY|GENE EXPRESSION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6586,"idioma_id":4,"titulo":"Loop B serine of a plasma membrane aquaporin type PIP2 but not PIP1 plays a key role in pH sensing","resumen":"In the plant kingdom, the plasma membrane intrinsic aquaporins (PIPs) constitute ahighly conserved group of water channels with the capacity of rapidly adjusting thewater permeability (Pf) of a cell by a gating response. Most evidence regarding thismechanism was obtained by different biophysical approaches including thecrystallization of a Spinaca olaracea PIP2 aquaporin (SoPIP2;1) in an open and closeconformation. A close state seems to prevail under certain stimuli such as cytosolic pHdecrease, intracellular Ca2+ concentration increase and dephosphorylation of specificSerines. In this work we decided to address whether the state of phosphorylation of aloop B Serine -highly conserved in all PIPs- combined with cytosolic acidification canjointly affect the gating response. To achieve this goal we generated loop B Serinemutants of two PIP types of Fragaria x ananassa (FaPIP2;1S121A andFaPIP1;1S131A) in order to simulate a dephosphorylated state and characterize theirbehavior in terms of Pf and pH sensitivity. The response was tested for different coexpressionsof PIPs (homo and heterotetramers combining wild-type and mutant PIPs)in Xenopus oocytes. Our results show that loop B Serine phosphorylation status affectspH gating of FaPIP2;1 but not of FaPIP1;1 by changing its sensitivity to more alkalinepHs. Therefore, we propose that a counterpoint of different regulatory mechanisms -heterotetramerization, serine phosphorylation status and pH sensitivity- affectaquaporin gating thus ruling the Pf of a membrane that expresses PIPs when fastresponses are mandatory.","palabras_clave":"WATER CHANNELS|OSMOTIC PERMEABILITY|WATER TRANSPORT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6588,"idioma_id":4,"titulo":"Experimental and theoretical investigation of the enantioselective hydrogenation of ethyl pyruvate with a Pt catalyst with new non-cinchona chiral modifiers","resumen":"The enantioselective hydrogenation of ethyl pyruvate using a Pt\/SiO2catalyst modified with sixdifferent chiral modifiers was studied. The chiral modifiers chosen were: (S)-(+)-1-aminoindan,(R)-(&#8722;)-1-aminoindan, (1R,2S)-(+)-cis-1- amino-2-indanol, (1S,2R)-(&#8722;)-cis-1-amino-2-indanol, (S)-(+)-1-indanol and (R)-(&#8722;)-1-indanol.An excess of the (R) enantiomer of the product of 63% and 45% with(S)-(+)-1-aminoindan and (R)-(&#8722;)-1-aminoindan modifiers, respectively was obtained. When using(1S,2R)-(&#8722;)-cis-1-amino-2-indanol and (1R,2S)-(+)-cis-1-amino-2-indanol, the enantiomeric excess (ee%)obtained was 30% and 5%, respectively, while with both indanols ee% did not exceed 8%. Molecularmodeling of the complex formed between the chiral modifier and ethyl pyruvate performed by DFTcalculations allowed predicting the values of ee% obtained experimentally. The low ee% value obtainedboth aminoindanol chiral modifiers were used, could be explained by the analysis of non-covalent inter-actions (NCI) method. These calculations demonstrated the presence of an intramolecular hydrogen bondin the structure of these modifiers.","palabras_clave":"ENANTIOSELECTIVITY|ETHYL PYRUVATE|NON-CINCHONA MODIFIERS|DFT|PLATINUM"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6589,"idioma_id":4,"titulo":"Platelet Apoptosis in Adult Immune Thrombocytopenia: Insights into the Mechanism of Damage Triggered by Auto-antibodies","resumen":"Mechanisms leading to decreased platelet count in immune thrombocytopenia (ITP) are heterogeneous. This study describes increased platelet apoptosis involving loss of mitochondrial membrane potential (&#916;&#936;m), caspase-3 activation (aCasp3) and phosphatidylserine (PS) externalization in a cohort of adult ITP patients. Apoptosis was not related to platelet activation, as PAC-1 binding, P-selectin exposure and GPIb-IX internalization were not increased. Besides, ITP platelets were more sensitive to apoptotic stimulus in terms of aCasp3. Incubation of normal platelets with ITP plasma induced loss of &#916;&#936;m, while PS exposure and aCasp3 remained unaltered. The increase in PS exposure observed in ITP platelets could be reproduced in normal platelets incubated with ITP plasma by adding normal CD3+ lymphocytes to the system as effector cells. Addition of leupeptin -a cathepsin inhibitor- to the system reverted the PS exposure. The apoptotic effect was also observed when normal platelets and CD3+ lymphocytes were incubated with purified IgG from ITP patients and was absent when ITP plasma was depleted of auto-antibodies, pointing to the latter as responsible for platelet damage. Apoptosis was present in platelets from all patients carrying anti-GPIIb-IIIa and anti-GPIb auto-antibodies but was absent in the patient with anti-GPIa-IIa auto-antibodies. Platelet damage inversely correlated with platelet count and decreased during treatment with a thrombopoietin receptor agonist. These results point to a key role for auto-antibodies in platelet apoptosis and suggest that antibody-dependent cell cytotoxicity is the mechanism underlying this phenomenon.","palabras_clave":"APOPTOSIS|PTI|ADCC|AUTOANTICUERPOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6590,"idioma_id":4,"titulo":"The commodity nature of labour-power","resumen":"Some recent Marxist contributions, among them the socalledNew Solution to the ´transformation problem´, call into questionthe idea of labor-power as a fully-fledged commodity. Yet, the rejectionof the commodity nature of labor-power compromises Marx´s wholeexplanation of the origin of surplus-value on the basis of the exchangeof equivalents. It can be shown, however, that it is possible to offer apositive case for the commodity-nature of labor-power which isconsistent with Marx´s broader dialectical investigation of thedeterminations of the value-form. This requires building upon thearguments that Marx explicitly put forward in his economic works, butalso going beyond them, albeit on the basis of those argumentsthemselves. Furthermore, this novel approach that treats thereproduction of labor-power as a commodity determined by the selfvalorizationof capital proves to be very valuable in shedding light ontwo classic Marxist controversies, namely: the debate on domestic laborand the one on skilled labor.","palabras_clave":"Commodity-form|Labour-Power|Value|Reproduction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6591,"idioma_id":5,"titulo":"Trabajo complejo y producción de valor en la crítica de la economía política","resumen":"En este artículo se realiza una reconstrucción crítica de la historia de la controversia en torno a la solución que ofrece Marx al problema de la determinación del trabajo complejo en la producción de valor y se presenta una solución alternativa que procura ser consistente con los fundamentos de la crítica marxiana de la economía política. El argumento principal es, en primer lugar, que las diferencias en la complejidad del trabajo están borradas en la forma de valor por medio de la determinación del trabajo objetivado en la mercancía como un trabajo simple. Y, en segundo lugar, que la determinación de la proporción en que el trabajo complejo se representa en más valor está dada exclusivamente por el gasto de fuerza de trabajo simple que tiene que realizar el trabajador calificado para producir su propia fuerza de trabajo.","palabras_clave":"TRABAJO COMPLEJO|TRABAJO SIMPLE|TEORIA DEL VALOR|DEBATE MARXISTA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6592,"idioma_id":4,"titulo":"Crustal deformation across the Southern Patagonian Icefield observed by GNSS","resumen":"Geodetic GNSS observations at 43 sites well distributed over the Southern Patagonian Icefield region yield site velocities with a mean accuracy of 1 mm\/a and 6 mm\/a for the horizontal and vertical components, respectively. These velocities are analyzed to reveal the magnitudes and patterns of vertical and horizontal present-day crustal deformation as well as their primary driving processes. The observed vertical velocities confirm a rapid uplift, with rates peaking at 41 mm\/a, causally related to glacial-isostatic adjustment (GIA). They yield now an unambiguous preference between two competing GIA models. Remaining discrepancies between the preferred model and our observations point toward an effective upper mantle viscosity even lower than 1.6 · 1018 Pa s and effects of lateral rheological heterogeneities. An analysis of the horizontal strain and strain-rate fields reveals some complex superposition, with compression dominating in the west and extension in the east. This deformation field suggests significant contributions from three processes: GIA, a western interseismic tectonic deformation field related to plate subduction, and an extensional strain-rate field related to active Patagonian slab window tectonics.","palabras_clave":"CRUSTAL DEFORMATION|GLACIAL ISOSTATIC ADJUSTMENT|GNSS|PATAGONIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6593,"idioma_id":4,"titulo":"Preparation And Characterization Of Polyvinyl Alcohol-pectin Cryogels Containing Enrofloxacin And Keratinase As Potential Transdermal Delivery Device","resumen":"Polyvinyl alcohol (PVA) and polyvinyl alcohol-pectin (PVA-P) cryogels are potential devices for wound healing. These materials have advantages over commercial wound dressings such as: retaining an appropriate level of moisture around wound and gas permeability and antibacterial properties. In a previous work, PVA-P cryogels containingan antibiotic and an enzyme (enrofloxacin and keratinase respectively) have been developed with promising results. In the present work, an exhaustive chemical, morphological and physical characterization of these films was carried out in order to explain the effect of incorporation of pectin into PVA matrix. The results show that the presence of pectin in PVA cryogel increase the size of PVA nanodomains determined by XRD patterns indicating an interaction between both PVA and pectin polymers. PVA nanodomains and crystallinity degree changed when enrofloxacin and keratinase were immobilized into PVA-P cryogels determined by SAXS and DSC analysis. These results suggest a good incorporation of both drugs into the polymeric matrix. A model involving the complex formation between the enzyme and enrofloxacin allocated through and between PVA nanodomains is proposed and it correlates well with results obtained in previous work where enrofloxacin kinetic release was found retarded by the presence of keratinase in the cryogels.","palabras_clave":"PVA|Pectin|Enrofloxacin|Keratinase|Cryogel"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6594,"idioma_id":4,"titulo":"A simple green route to obtain poly(vinyl alcohol) electrospun mats with improved water stability for use as potential carriers of drugs","resumen":"Poly(vinyl alcohol) (PVA) is a hydrophilic, biocompatible and nontoxic polymer. However, because of its low water-resistance, some applications for PVA-based materials are limited (e.g., drug delivery systems and wound dressings). In the current work, PVA mats containing tetracycline hydrochloride (TC) were successfully developed by electrospinning. In order to improve the water stability of the systems, the cross-linking of the PVA matrix was induced by citric acid (CA) addition together with heating treatments (150°C or 190°C for 3 min). TC presence led to a strong increase in the electrical conductivity of the blends and as a result, fibers with about 44% lower diameter (270 nm) than that of the corresponding unloaded mats (485 nm) were obtained. Laser scanning confocal microscopy images indicated that TC was well distributed along the PVA nanofibers. The mats were evaluated by FTIR, which revealed chemical interactions between PVA hydroxyl groups and CA carboxylic ones. The treatment at 150°C for 3 min proved to be the more suitable for the preparation of TC-containing mats with improved water resistance, maintaining the TC antimicrobial activity against both Escherichia coli and Staphylococcus aureus almost unaltered. These mats showed a burst release of TC, giving around 95% of the drug within the first hour of immersion in water.","palabras_clave":"PVA|electrohilado|tetraciclina|drug delivery"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6595,"idioma_id":5,"titulo":"Estudio de las intervenciones de un mismo terapeuta con 20 pacientes en la primera sesión","resumen":"Los autores se proponen estudiar las funciones (introductoria, de sintonía, de nexos, complementaria) y los deseos en las palabras de las intervenciones de un mismo terapeuta en la primera sesión con 20 diferentes pacientes. Realizan tres estudios: 1) lasvariaciones en el porcentaje de los tipos de intervenciones clínicas (tomando en cuenta sus funciones) a lo largo de una sesión dividida en tercios (inicial, medio, final), 2) el percentil de los deseos en las palabras del terapeuta en cada tipo de intervención, y 3) las variaciones en cuanto a los percentiles de los deseos en las palabras en cada tipo de intervención a lo largo de la sesión dividida en tercios. Cada uno de estos estudios agrega algo más de precisión y refinamiento al previo y los resultados de todos ellos muestran que los cambios del terapeuta en el curso de la sesión son acordes con el enfoque clínico.","palabras_clave":"Cambios en la actividad del terapeuta.|Función de las intervenciones clínicas |Deseos del terapeuta"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6596,"idioma_id":7,"titulo":"La unidad de palabra y persona. Aproximaciones a la relación educativa desde E. Levinas","resumen":"Este artículo pretende señalar posibilidades actuales de la educación desde una perspectiva filosófica, a partir de textos tempranos de E. Levinas. La perspectiva es fenomenológica, si bien en el desarrollo del trabajo queda de manifiesto que Levinas, ya en sus trabajos de posguerra, intenta ir más allá de la fenomenología, en tanto la conciencia se ve excedida por esta relación que se propone exponer. El Otro de la relación es considerado inicio tanto del mensaje que puede llegar desde la exterioridad, como de la propia identidad de quien lo recibe. En esa comunicación se manifiesta también la relación con «el maestro» y sus implicancias éticas y políticas. El sentido que Levinas da a los términos ética y religión, debe ser cuidadosamente expuesto para comprender que de lo que se trata por antonomasia en la enseñanza es la constitución de la estructura subjetiva frente al Otro que demanda. (La publicación del trabajo fue aprobada por referato en mayo de 2016)","palabras_clave":"LEVINAS|LENGUAJE|EDUCACION|ETICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6597,"idioma_id":4,"titulo":"Glyphosate Resistance in Perennial Ryegrass (Lolium perenne L.) is Associated with a Fitness Penalty","resumen":"The net selection effect of herbicides on herbicide resistance traits in weeds is conditioned by&nbsp; 13the fitness benefits and costs associated with resistance alleles. Fitness costs play an&nbsp; 14important evolutionary role preventing the fixation of adaptive alleles and contributing to the&nbsp; 15maintenance of genetic polymorphisms within populations. Glyphosate is widely used in&nbsp; 16world agriculture leading to the evolution of widespread glyphosate resistance in many weed&nbsp; 17species. The fitness of glyphosate-resistant and susceptible perennial ryegrass plants selected&nbsp; 18from within a single population were studied in two field experiments conducted during 2011&nbsp; 19and 2012 under different soil water availability.&nbsp; 20Glyphosate-resistant plants showed a reduction in height of 12 % and 16 %, leaf blade area of&nbsp; 2116 % and 33 %, shoot biomass of 45 % and 55 %, seed number of 33 % and 53 % and total&nbsp; 22seed mass of 16 % and 5 % compared to glyphosate susceptible plants in 2011 and 2012,&nbsp; 23respectively. The reduction in seed number per plant resulted in a 40 % fitness cost associated&nbsp; 24with the glyphosate resistance trait in perennial ryegrass. Fitness costs of glyphosate-resistant&nbsp; 25ManuscriptClick here to download Manuscript: Fitness costs ms (R2).docx 2plants were expressed under both conditions of water availability. These results could be&nbsp; 26useful for designing management strategies and exploit the reduced glyphosate resistant&nbsp; 27perennial ryegrass fitness in the absence of glyphosate selection.","palabras_clave":"fitness|resistance|lolium|glyphosate"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6598,"idioma_id":4,"titulo":"Tissue plant culture as a novel industrial strategy to produce biopharmaceuticals from endangered plants","resumen":"DOI 10.20472\/IAC.2016.024.030ISBN 978-80-87927-25-0, IISESGlobal natural antioxidants market is anticipated to grow on account of its increasing demand in food & beverages, cosmetics, pharmaceutical and animal feed. In this regard, we found high content of flavonoids and antioxidant activity (including antioxidant oligoelements) in the seeds of Araucaria araucana (piñones). However, bulk production of these antioxidants is ecologically non-viable since A. araucana is assessed as endangered species with increased extinction risk based in part by an extensive human harvesting of edible piñones. In this context, plant cell culture represents a useful production alternative to direct extraction of valuable secondary metabolites because: (a) a stable and uniform year-round supply of seed tissues or cells is guaranteed since biomass can be continuously produced in vitro, independent of seasonal variations, (b) selected compounds can be produced under controlled conditions, and moreover, (c) industrial production can be achieved while preserving the species. On the other hand, healthy plants can be easy obtained by micropropagation and then, the new plants can be acclimated to replant degraded areas of logged forest. At present, tissue and cell cultures from leaves and seeds, as well as plants, had been obtained in vitro from A. araucana and other native species. Furthermore, data of optimum conditions for in vitro production of antioxidants are being collected. The aim of these experiments is determine the varieties more adequate for the sustainable bio-based production of natural antioxidants by eco-efficient bio-processes and renewable bioresources.","palabras_clave":"ANTIOXIDANTS|CONSERVATION|SUSTAINABILITY|BIOECONOMY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6599,"idioma_id":4,"titulo":"Photochemical radical thiol-ene click-based methodologies for silica and transition metal oxides materials chemical modification: a mini-review","resumen":"Although known for more than 40 years in the polymer chemistry field, the photochemical radical thiol?ene addition (PRTEA) has been recently recognized as a chemical reaction with click characteristics. Photoinitiation enables spatial and temporal control of this highly efficient reaction, bridging simple organic chemistry with high-end materials synthesis and surfaces functionalization. In this minireview, we focus on the latest contributions based on the PRTEA for the synthesis of chemical precursors for silica and transition metal oxides (TMO) based materials. We summarize the mechanism of the PRTEA, the development of new families of photoinitiators and how this extremely simple approach has spilled over into the materials science arena with clear success. In particular, PRTEA adds to the collective efforts for building a reliable and straightforward chemical toolbox for surface modification and the production of sol?gel precursors, nanoparticles and thin films. The excellent perspectives for simple molecular and supramolecular building block synthesis opens up a rational synthetic route for the design and integration of these components in multipurpose platforms.","palabras_clave":"CLICK CHEMISTRY|FUNCTIONALIZATION|SILICA|METAL OXIDES|THIOL-ENE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6600,"idioma_id":5,"titulo":"El Ordenamiento Territorial y la gestión del Patrimonio Cultural del partido de Mercedes, Buenos Aires, Argentina","resumen":"Se compila y sistematiza la información correspondiente a los sitios de interés patrimonial del partido de Mercedes (Buenos Aires, Argentina) como parte necesaria para tener en cuenta para el Ordenamiento Territorial. La información se clasificó en: sitio arqueológico, yacimiento paleontológico, y sitios históricos (monumentos, edificios y lugares históricos). Se incluyeron variables tales como normativa de protección (municipal, provincial y nacional) y estado de preservación actual. Se identificaron un total de 113 sitios de interés patrimonial para el partido de Mercedes. Se observó que muchos de los lugares señalados como ?patrimonio cultural? por la ciudadanía, no cuentan con declaración patrimonial formal ni reglamentación de protección.","palabras_clave":"Patrimonio Cultural|SIG Patrimonial|Ordenamiento Territorial|Mercedes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6601,"idioma_id":4,"titulo":"Trypanosoma cruzi Proline Transport Presents a Cell Density-dependent Regulation","resumen":"Trypanosoma cruzi, the etiological agent of Chagas disease, uses proline as itsmain carbon source, essential for parasite growth and stage differentiation inepimastigotes and amastigotes. Since proline is mainly obtained from extracellularmedium by transport proteins, in this work we studied the regulation ofthe T. cruzi proline transporter TcAAAP069. Proline uptake and intracellularconcentration presented oscillations during epimastigote growth phases,increasing during the early exponential phase (322 pmol\/min) and decreasingto undetectable levels during the late exponential phase. Transporter expressionrate correlated with proline uptake, and its subcellular localization alternatedfrom both, the plasma membrane and close to the flagellar pocket,when the transport is higher, to only the flagellar pocket region, when thetransport decreased until proline uptake and TcAAAP069 protein became undetectableat the end of the growth curve. Interestingly, when parasites weretreated with conditioned medium or were concentrated to artificially increasethe culture density, the proline transport was completely abolished resemblingthe effects observed in late exponential phase. These data highlight for thefirst time the existence of a density-associated regulation of relevant physiologicalprocesses such as proline metabolism.","palabras_clave":"Trypanosoma cruzi|Transporters|Proline|Chagas Disease"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6602,"idioma_id":4,"titulo":"Resveratrol inhibits Trypanosoma cruzi arginine kinase and exerts a trypanocidal activity","resumen":"Arginine kinase catalyzes the reversible transphosphorylation between ADP and phosphoarginine which plays a critical role in the maintenance of cellular energy homeostasis. Arginine kinase from the protozoan parasite Trypanosoma cruzi, the etiologic agent of Chagas disease, meets the requirements to be considered as a potential therapeutic target for rational drug design including being absent in its mammalian hosts. In this study a group of polyphenolic compounds was evaluated as potential inhibitors of arginine kinase using molecular docking techniques. Among the analyzed compounds with the lowest free binding energy to the arginine kinase active site (<&#8722;6.96 kcal\/mol), resveratrol was chosen for subsequent assays. Resveratrol inhibits 50% of recombinant arginine kinase activity at 325 &#956;M. The trypanocidal effect of resveratrol was evaluated on the T. cruzi trypomastigotes bursting from infected CHO K1 cells, with IC50 = 77 &#956;M. Additionally epimastigotes overexpressing arginine kinase were 5 times more resistant to resveratrol compared to controls. Taking into account that: (1) resveratrol is considered as completely nontoxic; (2) is easily accessible due to its low market price; and (3) has as a well-defined target enzyme which is absent in the mammalian host, it is a promising compound as a trypanocidal drug for Chagas disease.","palabras_clave":"Resveratrol|Trypanosoma cruzi|Arginine kinase"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6603,"idioma_id":4,"titulo":"Cryopreservation by slow cooling of rat neuronal cells","resumen":"Although primary neuronal cells are routinely used for neuroscience research, with potential clinical applications such as neuronal transplantation and tissue engineering, a gold standard protocol for preservation has not been yet developed. In the present work, a slow cooling methodology without ice seeding was studied and optimized for cryopreservation of rat cerebellar granular cells. Parameters such as cooling rate, plunge temperature and cryoprotective agent concentration were assessed using a custom built device based on Pye´s freezer idea. Cryopreservation outcome was evaluated by post thawing cell viability\/viable cell yield and in culture viability over a period of 14&nbsp;days. The best outcome was achieved when 10% of Me2SO as cryoprotective agent, a cooling rate of 3.1&nbsp;±&nbsp;0.2&nbsp;°C\/min and a plunge temperature of&nbsp;&#8722;48.2&nbsp;±&nbsp;1.5&nbsp;°C were applied. The granular cells cryopreserved under these conditions exhibited a cell viability of 82.7&nbsp;±&nbsp;2.7% and a viable cell yield of 28.6&nbsp;±&nbsp;2.2%. Moreover, cell viability in culture remained above 50%, very similar to not cryopreserved cells (control). Our results also suggest that post-thaw viability (based on membrane integrity assays) not necessarily reflects the quality of the cryopreservation procedure and proper functionality tests must be carried out in order to optimize both post thaw viability\/cell yield and in culture performance.","palabras_clave":"neuronal cells|slow cooling|cryopreservation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6604,"idioma_id":4,"titulo":"New host and distribution for the mosquito parasite Strelkovimermis spiculatus.","resumen":"Strelkovimermis spiculatus, Poinar and Camino 1986 (Nematoda: Mermithidae) was found parasitizing some mosquito genera like Aedes (Ochlerotatus), Culex and Psorophora. In a mosquito distribution project in Buenos Aires, Argentina, we found nematodes infecting mosquito larvae in natural breeding sites in the outskirts of Mar del Plata city. The aim of this work was to identify this parasite in this distribution area and determine the mosquito species host. COI and 18S rRNA-ITS1-5.8S rRNA-ITS2-28S rRNA fragment genes were described and were used for molecular identification of this nematode, confirming the morphological diagnostic traits. In this report a new host of S. spiculatus, the mosquito larvae of Culex eduardoi was detected, expanding the southeastern distribution.","palabras_clave":"NEMATODES|NEW RECORD|Culex eduardoi"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6605,"idioma_id":5,"titulo":"AGREGADOS RECICLADOS PRETRATADOS PARA USO EN HORMIGÓN","resumen":"Los residuos de laconstrucción, principalmente aquellos que provienen de la trituración dehormigones de demolición, pueden ser utilizados como reemplazo total o parcialdel agregado natural. Como consecuencia de su mayor porosidad, el agregado reciclado resultamenos denso y con una mayor capacidad de absorción de agua que el natural.En este trabajo se presentan distintos tratamientos de impregnación sobreel agregado grueso reciclado de un hormigón de canto rodado, con el fin demejorar la calidad del hormigón resultante y optimizar su uso. Se analizaron tres tipos de productos para tratar al agregado reciclado (dos comerciales de usofrecuente en el mercado y una pasta cementícea) y diferente tiempo entre laejecución del tratamiento y su incorporación al hormigón. Se estudiaron propiedadesmecánicas y físicas de hormigones con un reemplazo del 50% del agregado gruesonatural por hormigón triturado. Se realizó un estudio petrográfico sobre las diferentes muestras a fin dedeterminar la influencia de los tratamientos en la morfología de la zona deinterfase agregado-mortero. El tratamiento previo deimpregnación del agregado reciclado demostró ser una alternativa para mejorarla estructura de poros del hormigón y llevarlo a un comportamiento similar alde un hormigón convencional. El uso de un material de desecho permite reducir la cantidad de residuosgenerados una vez cumplida la vida útil de las estructuras y disminuir laexplotación de recursos naturales no renovables como son los agregados parahormigón.","palabras_clave":"Agregado reciclado|Hormigón|Tratamiento previo|Petrografía"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6606,"idioma_id":4,"titulo":"The &#8722;2518 A\/G polymorphism in the monocyte chemoattractant protein 1 gene (MCP-1) is associated with an increased risk of rheumatoid arthritis in Argentine patients","resumen":"The aim of this study was to analyze the influence of nucleotide transition (G\/A) in position &#8722;2518 of the MCP-1 gene related to the susceptibility of developing RA. Two hundred twenty-three consecutive RA patients according to 2010 ACR\/EULAR criteria were included; 120 healthy subjects were used as controls. MCP-1 &#8722;2518 A\/G polymorphism (AG + GG) was present in 162 (72.6 %) RA patients and in 63 (52.5 %) healthy subjects [OR 2.44 (IC95% 1.53?3.88, p = 0.0002)]; associations for heterozygotes and homozygotes were OR 1.92 (IC95% 1.19?3.15, p = 0.001) and OR 5.19 (IC95% 2.34?11.51, p = 0.001), respectively. In Argentine patients, MCP-1 gene polymorphism confers susceptibility for developing RA.","palabras_clave":"Genetics  Methodology|rheumatoid arthritis |Rheumatic diseases|immunology"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6607,"idioma_id":5,"titulo":"Determinación de la presencia de contusiones en reses de novillitos faenados en un frigorífico con habilitación provincial","resumen":"El manejo que se realiza al ganado bovino en las horas previas a su faena tiene efectos estresantes sobre los animales, pudiendo afectar la cantidad y calidad de la carne producida.El transporte inadecuado y los malos tratos previos al sacrificio provocan contusiones. El objetivo de este trabajo fue determinar la presencia de contusiones en reses de novillitos faenados en un frigorífico con habilitación provincial. Se evaluaron 620 reses. Se registró el peso de cada res y se realizó la tipificación según su conformación y estado de engrasamiento. Posteriormente, se determinó la presencia de contusiones, las que se caracterizaron según su tamaño y ubicación anatómica. El peso promedio de las reses fue 223,9 kg. La conformación fue intermedia y homogénea, registrándose un 78,1% de reses tipo C. Con respecto al estado de engrasamiento, se observó un 31,9% de reses con calificación 1 y 64,2% con calificación 2. El 70,5% presentaban una o más contusiones. El 46,7% de las reses lesionadas tenía 1 contusión, siguiendo en importancia las reses con 2 (29,1%) y 3 (14,6%) contusiones. El 56,3% de las contusiones tenían un diámetro menor a 5 cm. Las regiones anatómicas con mayor frecuencia de lesiones fueron el cuarto (54,4%) y el lomo (23,3%). La alta proporción de reses con contusiones, así como la cantidad promedio de contusiones por res son claros indicadores de la necesidad de mejorar el trato de los animales durante la carga, transporte, descarga en el frigorífico y espera previa a su faena.","palabras_clave":"BIENESTAR ANIMAL|CONTUSIONES|NOVILLITOS|RESES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6608,"idioma_id":5,"titulo":"Reparticiones, saberes y técnicos para un Estado provincial: Leopoldo Suárez, de agrónomo a funcionario multifacético del lencinismo, Mendoza, 1918-1930","resumen":"Los abordajes sobre las reparticiones públicas y las burocracias que las componen permiten desmenuzar las dinámicas y actores que integran un Estado en constante proceso de transformación y complejización. Desde esta perspectiva, y atendiendo a las particularidades de un Estado provincial, en este trabajo nos detenemos en la trayectoria de un enólogo e ingeniero agrónomo Leopoldo Suárez, quien entre 1918 y 1930 se habría consolidado como el epicentro de un núcleo de técnicos y profesionales liberales que respaldaron y legitimaron el proyecto económico, social y político de la Unión Cívica Radical lencinista en Mendoza. Entre otros aspectos, el artículo concluye que su trayectoria pública osciló entre la injerencia directa en el sector vitivinícola -incluso defendiendo convicciones que e costaron el enfrentamiento con el sector más poderoso de la burguesía vitivinícola-, el estudio e impulso de obras públicas (irrigación), y la reforma educativa, tanto desde el ejecutivo como desde el Poder Legislativo provincial.","palabras_clave":"MENDOZA|LEOPOLDO SUÁREZ|BUROCRACIAS ESTATALES|ECONOMÍA PRODUCTIVA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6609,"idioma_id":5,"titulo":"Estudio del juego adulto-niño en edad preescolar. Diferentes sistemas de observación","resumen":"Se presenta un aspecto del programa de investigación que este grupo viene realizando desde el año 2000 en el marco de las diferentes programaciones UBACyT. En esta etapa nos proponemos realizar articulaciones entre nuestro quehacer como psicoanalistas clínicos especializados en primera infancia y la labor de investigación basada en observaciones minuciosas de interacciones video-filmadas. Ejemplificamos, a partir de las viñetas de situaciones lúdicas videofilmadas en el marco de la investigación , la superposición de análisis posibles de las mismas desde tres sistemas, desarrollados por el equipo, descriptos también en este artículo.  Un primer sistema estudia la modalidad afectiva de interacción, los indicadores de desregulación y el nivel de simbolización. Un segundo sistema se centra en los temas más frecuentes que se despliegan en la situación de juego libre. El tercero se basa en conceptualizaciones psicoanalíticas, con énfasis en la teoría de desarrollo de la libido. Tenemos en cuenta una visión que contempla la complejidad y la heterogeneidad de nuestro objeto de estudio y el aporte que la investigación observacional de interacciones lúdicas progenitor-niño puede realizar al estudio del desarrollo y a la clínica en primera infancia.","palabras_clave":"Juego|Sistema de observación|Simbolización|Desarrollo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6610,"idioma_id":4,"titulo":"Towards to a Predictive Model of Academic Performance Using Data Mining in the UTN - FRRe","resumen":"Students completing the courses requiredto become an Engineer in InformationSystems in the Resistencia Regional Faculty,National Technological University,Argentine (UTN-FRRe), face the challengeof attending classes and fulfillingcourse regularization requirements, oftenfor correlative courses. Such is the case offreshmen´s course Algorithms and DataStructures: it must be regularized in orderto be able to attend several second andthird year courses. Based on the results ofthe project entitled ?Profiling of studentsand academic performance through theuse of data mining?, 25\/L059 - UTI1719,implemented in the aforementionedcourse (in 2013-2015), a new project hasstarted, aimed to take the descriptiveanalysis (what happened) as a startingpoint, and use advanced analytics, tryingto explain the why, the what will happen,and how we can address it. Different datamining tools will be used for the study:clustering, neural networks, Bayesiannetworks, decision trees, regression andtime series, etc. These tools allow differentresults to be obtained from differentperspectives, for the given problem. Inthis way, potential problematic situationswill be detected at the beginning ofcourses, and necessary measures can betaken to solve them. Thereby, the aim ofthis projects is to identify students whoare at risk of abandoning the race to givespecial support and avoid that situation.Decision trees as predictive classificationtechnique is mainly used.Keywords: academic performance; datawarehouses; data mining; predictivemodels.","palabras_clave":"academic performance|data warehouses|data mining|predictive models"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6611,"idioma_id":4,"titulo":"Effect of cover crops on microbial community structure and related enzyme activities and macronutrient availability","resumen":"Continuous monoculture in major agricultural regions has been characterized by the loss of fertility, increased soil erosion and surface runoff. Thus, the inclusion of cover crops (CC) is a promising option aimed at better preserving the sustainable production of agricultural systems. The objective of this study was to assess the short-term effect of CC on soil microbial community structure and related enzyme activities and macronutrient availability. Species tested as CC were: oat (Avena sativa L.), vetch (Vicia sativa L.) and radish (Raphanus sativus L.), which were sown in two species mixtures, oat\/radish (CC1) and oat\/radish\/vetch (CC2), with soybean monoculture and soybean\/corn being the cash crops. The field trial was performed under no-tillage, and soil sampling was carried out in 2013 and 2014. The analysis of soil phospholipid fatty acid (PLFA) showed an increase of total bacterial PLFA and Gram-positive PLFA under CC treatments, being on average 6.8% significantly higher in CC2 and CC1 than in the control treatment. In addition, soil enzyme activities (esterase activity, dehydrogenase activity and acid phosphatase activity) were on average 20% higher in plots under CC in comparison to control treatments. The total N was significantly higher after CC2 treatment (3.13 mg g&#8722;1) than in either the CC1 (2.00 mg g&#8722;1) or control (2.19 mg g&#8722;1). This research revealed that the inclusion of CC mixtures in crop rotation produced an increase in bacterial PLFA, in particular Gram-positive bacteria, in the short term. These changes were related to soil enzyme activities and to the availability of the main macronutrients N and P, with a CC mixture including oat\/radish\/vetch being highly recommended not only to improve soil biological processes but also to provide additional N to the cash crop. © 2016 Elsevier Masson SAS","palabras_clave":"soil|functionality|biodiversity|sustainability"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6612,"idioma_id":5,"titulo":"Multiterritorialidad en la Patagonia rural esteparia Destinos de la tierra, ausentismo, y problemáticas emergentes","resumen":"El artículo tiene por principal objetivo presentar un avance analítico respecto de la Patagonia de la estepa que, en circunstancias muchas veces críticas, desde fines de los años ochenta es atravesada por situaciones novedosas que condicionan su organización espacial. Se incorporan los conceptos de multiterritorialidad y ausentismo propietario como camino explicativo de nuevas configuraciones. Se concentró el análisis en estudios de casos en concordancia con estrategias de relevamientos para la identificación de las dinámicas espacio-temporales y los destinos de los espacios. El enfoque metodológico general es mixto, de procedimientos cuantitativos -bases de datos y cartografía temática- y cualitativos sostenidos en múltiples entrevistas a actores representativos. Se concluye en cómo las lógicas territoriales obedecen a diferentes propósitos en distintas escalas, los que culminan en iniciativasproductivas y contemplativas\/recreativas asociadas a decisiones empresariales,institucionales y personales. En este marco contextual las incertidumbres son recurrentes, valorizándose entonces los aportes académicos en favor de colaborar a esclarecer el panorama rural con su proyección en las gestiones socioterritoriales","palabras_clave":"Transformaciones territoriales,|Declive ganadero|Lógicas territoriales |Organización espacial"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6613,"idioma_id":4,"titulo":"Hepatic carboxylesterase 3 (Ces3\/Tgh) is downregulated in the early stages of liver cancer development in the rat","resumen":"It is accepted that cancer development is associated with metabolic changes. Previously, we established a modelofhepaticpreneoplasiain which adultratswere subjected to a 2-phase modelof hepatocarcinogenesis(initiated-promoted, IP) for 6 weeks until they develop altered hepatic foci (AHF). Here, we found that a whole metabolicshift occurs in order to favor cancer development. IP animals presented with increased plasma lipids due to in-creased VLDL secretion as well as increased liver lipid accretion due to stimulated transacetylase activity ratherthan lipogenesis, compared to control rats. We found that carboxylesterase 3\/triacylglycerol hydrolase (Ces3\/Tgh) presented with a perilobular distribution surrounding lipid droplets in normal livers. However, it is down-regulated both at the protein and mRNA level in liver homogenates and is almost undetectable inside the AHFwith no changes in the surrounding tissue. Ces3\/Tgh expression is regulated by &#969;-3 fatty acids, thus, supplemen-tation of diet with fish oil, allowed the restoration of Ces3\/Tgh expression inside the foci and, more interestingly,led to the decrease in number and volume of the AHF. These studies show a preventive role of Ces3\/Tgh in livercancer development.","palabras_clave":"CARBOXILESTERASES|LIVER PRENEOPLASIA|HYPERTRIGLYCERIDEMIA|POLYUNSATURATED FATTY ACIDS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6614,"idioma_id":4,"titulo":"Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet","resumen":"High-grain feeding used in the animal production is known to affect the host rumen bacterial community, but our understanding of consequent changes in goats is limited. This study was therefore aimed to evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats to the high-grain diet (grain: hay ? ratio of 40:60). The dietary transition of goats from the forage to the high-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higher than value established for acute or subacute ruminal acidosis was not diagnosed in studied animals. DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain fed animals, but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fed animals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days after the incorporation of corn into the diet and significantly lower concentration of bacteria belonging to Actinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day 20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantly higher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fed animals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals. The three different culture independent methods used in this study show that high proportion of concentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicate the good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.","palabras_clave":"Creole goats| Rumen|Bacteria|PCR-DGGE|qPCR|NGS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6615,"idioma_id":4,"titulo":"The Genetic Analysis of an Acinetobacter johnsonii Clinical Strain Evidenced the         Presence of Horizontal Genetic Transfer.","resumen":"Acinetobacter johnsonii rarely causes human infections. While most A. johnsonii isolates are susceptible to virtually all antibiotics, strains harboring a variety of â-lactamases have recently been described. An A. johnsonii Aj2199 clinical strain recovered from a hospital in Buenos Aires produces PER-2 and OXA-58. We decided to delve into its genome by obtaining the whole genome sequence of the Aj2199 strain. Genome comparison studies on Aj2199 revealed 240 unique genes and a close relation to strain WJ10621, isolated from the urine of a patient in China. Genomic analysis showed evidence of horizontal genetic transfer (HGT) events. Forty-five insertion sequences and two intact prophages were found in addition to several resistance determinants such as blaPER-2, blaOXA-58, blaTEM-1, strA, strB, ereA, sul1, aacC2 and a new variant of blaOXA-211, called blaOXA-498. In particular, blaPER-2 and blaTEM-1 are present within the typical contexts previously described in the Enterobacteriaceae family. These results suggest that A. johnsonii actively acquires exogenous DNA from other bacterial species and concomitantly becomes a reservoir of resistance genes.","palabras_clave":"ACINETOBACTER|GENETIC|ANALYSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6616,"idioma_id":4,"titulo":"Vascular action of bisphosphonates: In vitro effect of alendronate on the regulation of cellular events involved in vessel pathogenesis","resumen":"In this work we investigate whether, despite the procalcific action of alendronate on bone, the drug would be able to regulate in vitro the main cellular events that take part in atherosclerotic lesion generation. Using endothelial cell cultures we showed that Alendronate (1?50 &#956;M) acutely enhances nitric oxide production (10?30 min). This stimulatory action of the bisphosphonate involves the participation of MAPK signaling transduction pathway. Under inflammatory stress, the drug reduces monocytes and platelets interactions with endothelial cells induced by lipopolysaccharide. Indeed the bisphophonate exhibits a significant inhibition of endothelial dependent platelet aggregation. The molecular mechanism of alendronate (ALN) on leukocyte adhesion depends on the regulation of the expression of cell adhesion related genes (VCAM-1; ICAM-1); meanwhile the antiplatelet activity is associated with the effect of the drug on nitric oxide production. On vascular smooth muscle cells, the drug exhibits ability to decrease osteogenic transdifferentiation and extracellular matrix mineralization. When vascular smooth muscle cells were cultured in osteogenic medium for 21 days, they exhibited an upregulation of calcification markers (RUNX2 and TNAP), high alkaline phosphatase activity and a great amount of mineralization nodules. ALN treatment significantly down-regulates mRNA levels of osteoblasts markers; diminishes alkaline phosphatase activity and reduces the extracellular calcium deposition. The effect of ALN on vascular cells differs from its own bone action. On calvarial osteoblasts ALN induces cell proliferation, enhances alkaline phosphatase activity, and increases mineralization, but does not affect nitric oxide synthesis. Our results support the hypothesis that ALN is an active drug at vascular level that regulates key processes involved in vascular pathogenesis through a direct action on vessel cells.","palabras_clave":"Alendronate|atherosclerosis|bone|osteogenic transdifferentiation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6617,"idioma_id":4,"titulo":"Solving minimax control problems via nonsmooth optimization","resumen":"We address minimax optimal control problems with linear dynamics. Under convexity assumptions, by using non-smooth optimization techniques, we derive a set of optimality conditions for the continuous- time case. We define an approximated discrete-time problem where analogous conditions hold. One of them allows us to design an easily implementable descent method. We analyze its convergence and we show some preliminary numerical results.","palabras_clave":"MINIMAX CONTROL PROBLEMS|NONSMOOTH OPTIMIZATION|OPTIMALITY CONDITIONS|NUMERICAL SOLUTIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6618,"idioma_id":5,"titulo":"Descripción de los problemas resueltos de corriente alterna en libros de texto universitarios","resumen":"Se exploró una muestra intencional de problemas resueltos, inherentes a circuitos de corriente alterna, que se muestran en diez libros de texto universitarios de uso frecuente en Argentina. Los resultados señalan que en la mayoría de los enunciados no se discute el interés del problema, el contexto en pocas ocasiones alude a la vida cotidiana, las figuras son escasas y las consignas solicitadas son cerradas y directas. En la mayor parte de las resoluciones no se especifica el sistema físico ni el modelo empleado. Predominan hipótesis analíticas y resoluciones formales con explicaciones breves. La cantidad de gráficos cartesianos y vectores rotantes es escasa. Los resultados se presentan generalmente en forma numérica y se interpretan o validan en menos de la mitad de los casos. Habitualmente no se plantean otras perspectivas de resolución ni nuevos interrogantes. Se concluye que los casos analizados estimularían el uso de algoritmos matemáticos y no promoverían el quehacer científico en los estudiantes. Resulta prioritario crear ambientes de aprendizaje donde se promueva la comprensión cualitativa de los problemas y el análisis de conceptos físicos subyacentes.","palabras_clave":"PROBLEMAS RESUELTOS|LIBROS DE TEXTO|UNIVERSIDAD|CIRCUITOS DE CA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6619,"idioma_id":4,"titulo":"Diacylglycerol pyrophosphate binds and inhibits the glyceraldehyde- 3-phosphate dehydrogenase in barley aleurone","resumen":"&amp;lt;!-- \/* Font Definitions *\/@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536859905 -1073711037 9 0 511 0;}@font-face{font-family:'Cambria Math';panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;} \/* Style Definitions *\/p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:'Times New Roman';mso-fareast-font-family:'Times New Roman';mso-ansi-language:EN-GB;}p.Default, li.Default, div.Default{mso-style-name:Default;mso-style-unhide:no;mso-style-parent:'';margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;mso-layout-grid-align:none;text-autospace:none;font-size:12.0pt;font-family:Arial;mso-fareast-font-family:'Times New Roman';color:black;mso-ansi-language:ES;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;}@page WordSection1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&amp;gt;The aleurona cell is a model that allows the study of the antagonisticeffect of gibberellic acid (GA) and abscisic acid (ABA). Previous results ofour laboratory demonstrated the involvement of phospholipids during the responseto ABA and GA. ABA modulates the levels of diacylglycerol, phosphatidic acidand diacylglycerol pyrophosphate (DAG, PA, DGPP) through the activities ofphosphatidate phosphatases, phospholipase D, diacylglycerol kinase andphosphatidate kinase (PAP, PLD, DGK and PAK). PA and DGPP are key phospholipidsin the response to ABA, since both are capable of modifying the hydroliticactivity of the aleurona. Nevertheless, little is known about the mechanism ofaction of these phospholipids during the ABA signal. DGPP is an anionicphospholipid with a pyrophosphate group attached to diacylglycerol. Theionization of the pyrophosphate group may be important to allow electrostaticinteractions between DGPP and proteins. To understand how DGPP mediates cellfunctions in barley aleurone, we used a DGPP affinity membrane assay to isolateDGPP-binding proteins from Hordeumvulgare, followed by mass spectrometric sequencing. A cytosolicglyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) was identifiedfor being bound to DGPP. To validate our method, the relatively abundant GAPDHwas characterized with respect to its lipid-binding properties, by fat westernblot. GAPDH antibody interacts with proteins that only bind to DGPP and PA. Wealso observed that ABA treatment increased GAPDH abundance and enzyme activity.The presence of phospholipids during GAPDH reaction modulated the GAPDHactivity in ABA treated aleurone. These data suggest that DGPP binds to GAPDHand this DGPP and GAPDH interaction provides new evidences in the study of DGPP-mediatedABA responses in barley aleurone. &nbsp;","palabras_clave":"GAPDH|PHOSPHATIDIC ACID|DIACYLGLYCEROL PYROPHOSPHATE|ABSCISIC ACID"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6620,"idioma_id":4,"titulo":"Saline and osmotic stresses stimulate PLD\/diacylglycerol kinase activities and increase the level of phosphatidic acid and proline in barley roots","resumen":"Soil salinity is one of the major abiotic stresses that affect the crop productivity. Understanding the mechanisms by which plants transmit the signals to cellular machinery to trigger adaptive responses is essential to develop more stress tolerant crops. Barley (Hordeum vulgare L.) is an important cereal and its production is affected by increasing dryland salinity. This severely limits growth and reduces yields. In barley seedling, NaCl and mannitol stresses modulated the level of phosphatidic acid (PA), the proline accumulation and the reduced root length. PA is a well-known lipid signal and an intermediary in the lipid synthesis. However, little is known about their role during the saline and osmotic stresses in barley roots. PA increased by phospholipase D (PLD, E.C. 3.1.4.4) and by diacylglycerol kinase activities (DAG-k, EC 2.7.1.107) and its conversion to DGPP suggested that they are part of stress responses to salinity in barley. In contrast, saline stress decreased the activity of the Mg2+-independent, NEM-insensitive form of phosphatidate phosphohydrolase (PAP2, E.C. 3.1.3.4), keeping the PA levels. The application of 1-butanol also stimulated proline accumulation while the DGK-inhibitor treatment decreased proline levels. Endogenous phytohormone levels measured by liquid chromatography-tandem mass spectrometry revealed that, under stress, the barley roots decreased the GA3 and ABA levels and increased the SA and JA endogenous amounts. The results presented here suggest that PA may modulate the cellular signal of barley roots by differentially affecting components of the abiotic stress ! response cascade.","palabras_clave":"PROLINE|PHOSPHOLIPASE D|LIPID KINASES|BARLEY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6621,"idioma_id":4,"titulo":"Clonidine complexation with hydroxypropyl-beta-cyclodextrin: From physico-chemical characterization to in vivo adjuvant effect in local anesthesia","resumen":"Clonidine (CND), an alpha-2-adrenergic agonist, is used as an adjuvant with local anesthetics. In this work,we describe the preparation and characterization of an inclusion complex of clonidine in hydroxypropyl-beta-cyclodextrin (HP-&#9252;-CD), as revealed by experimental (UV?vis absorption, SEM, X-ray diffraction,DOSY- and ROESY-NMR) and theoretical (molecular dynamics) approaches. CND was found to bindto HP-&#9252;-CD (K a = 20 M &#8722;1 ) in 1:1 stoichiometry. X-ray diffractograms and SEM images provided evi-dence of inclusion complex formation, which was associated with changes in the diffraction patternsof the pure compounds. NMR experiments revealed changes in the chemical shift of H3 HP-&#9252;-CD hydro-gens ( = 0.026 ppm) that were compatible with the insertion of CND in the hydrophobic cavity ofthe cyclodextrin. Molecular dynamics simulation with the three CND species that exist at pH 7.4revealed the formation of intermolecular hydrogen bonds, especially for the neutral imino form ofCND, which favored its insertion in the HP-&#9252;-CD cavity. In vitro assays revealed that complexationretarded drug diffusion without changing the intrinsic toxicity of clonidine, while in vivo tests in ratsshowed enhanced sensory blockade after the administration of 0.15% CND, with the effect decreasingin the order: CND:HP-&#9252;-CD + bupivacaine > CND + bupivacaine > bupivacaine > CND:HP-&#9252;-CD > clonidine.The findings demonstrated the suitability of the complex for use as a drug delivery system for clinicaluse in antinociceptive procedures, in association with local anesthetics.","palabras_clave":"CLONIDINE|CYCLODEXTRIN|DRUG DELIVERY|MAGNETIC RESONANCE|MOLECULAR DYNAMICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6622,"idioma_id":4,"titulo":"Dietary patterns, overweight and obesity from 1961 to 2011 in the socioeconomic and political context of Argentina","resumen":"To analyse the changes in eating patterns in Argentina from 1961 to 2011, and to assess changes in overweight and obesity in their socioeconomic and political context, we performed a hierarchical cluster analysis. We used the information from Food Balance Sheets of the United Nations Food and Agriculture Organisation to identify dietary patterns of apparent consumption. Years were grouped into five patterns. The food group with the highest apparent consumption was cereals (30% of total kcal\/person\/day) although this decreased slightly. Meats were second and their contribution decreased by 12%. The following foods contribution increased during the period: Sugar and milk by 2% and vegetable oils by 6%. The changes observed in the number of kcal\/person\/day were in line with changes in real wage, and coincided with economic and political crises that Argentina experienced during that period. Changes in eating patterns allow us to interpret that they relate to the increase in overweight and obesity.","palabras_clave":"DIETARY PATTERNS|OBESITY|OVERWEIGHT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6623,"idioma_id":4,"titulo":"Nematodes from Achatina fulica Bowdich, 1822 (Mollusca: Gastropoda) in Argentina","resumen":"ver PDF adjunto","palabras_clave":"Achatina fulica|intermediate host|Strongyluris sp.|parasite surveillance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6624,"idioma_id":4,"titulo":"Molecular characterization of trypanosomatid infections in wild howler monkeys (Alouatta caraya) in northeastern Argentina","resumen":"The transmission of Trypanosoma cruzi by vectors is confined to the Americas, and the infection circulates in at least two broadly defined transmission cycles occurring in domestic and sylvatic habitats. This study sought to detect and characterize infection by T. cruzi and other trypanosomes using PCR strategies in blood samples from free-ranging howler monkeys, Alouatta caraya, in the northeastern Argentina. Blood samples were collected at four sites with variable levels of habitat modification by human activity. PCR was conducted using primers for kinetoplast DNA, satellite DNA and ribosomal DNA of the trypanosomatid parasites. Ribosomal and satellite DNA fragments were sequenced to identify the trypanosomatid species and to characterize the discrete typing units (DTUs) of T. cruzi. Overall, 46% (50\/109) of the howlers were positive according to the kDNA-PCR assay, but only 7 of the howlers were positive according to the SatDNA-PCR protocol.We sequenced the amplicons of the satellite DNA obtained from five specimens, and the sequences were 99% and 100% similar to T. cruzi. A sequence typical of DTU T. cruzi I was found in one howler monkey from the ?remote? site, while sequences compatible with DTUs II, V, and VI were found in howlers from the ?remote?, ?rural? and ?village? sites. We detected 96% positive samples for RibDNA-PCR, 9 of which were sequenced and displayed 99% identity with Trypanosoma minasense, while none showed identity with T. cruzi. The results demonstrated the presence of T. cruzi and a species closely related to T. minasense in blood samples from free-ranging A. caraya, belonging todifferent T. cruzi DTUs circulating in these howler monkey populations. The results obtained in this study could help evaluate the role of A. caraya as a reservoir of T. cruzi in regions where Chagas disease is hyperendemic and where the human-wildlife interface is increasing.","palabras_clave":"TRYPANOSOMA CRUZI|ALOUATTA CARAYA|ENZOOTIC CYCLE|HOWLER MONKEY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6625,"idioma_id":5,"titulo":"La eco-epidemiología retrospectiva como herramienta aplicada a la vigilancia de la leishmaniasis en Misiones, Argentina, 1920-2014","resumen":"Se presenta una metodología analítica retrospectiva, basada en el marco teórico de la eco-epidemiología,anclada en una escala espacial subnacional. Esta metodología, aplicada aquí a la caracterización de escenarios de transmisión de la leishmaniasis en la provincia argentina de Misiones ?fronteriza con Brasil y Paraguay? permitió fundamentar recomendaciones de vigilancia y control apropiadas a dicha escala. Se realizó una búsqueda exhaustiva de la literaturasobre leishmaniasis en esa provincia y se determinaron tres escenarios de transmisión de leishmaniasis cutánea (LC) y visceral (LV), correspondientes a tres períodos: 1920-1997, en el que se constató la transmisión de LC, dispersa en el tiempo y el espacio; 1998-2005, en el que hubo brotes focales de LC; y 2006-2014 en el que, además, se registraron brotes y se documentó la dispersión geográfica de la LV. Para caracterizarlos escenarios de riesgo y los procesos antrópicos que los producen, losresultados se sintetizaron e integraron en el contexto socio-histórico y bio-ecológico de cada período analizado. Se fundamentan recomendaciones de vigilancia y control en el territorio estudiado, entre ellas, estableceruna vigilancia activa para monitorear posibles tendencias al incremento de lacirculación parasitaria y vectorial y, ante la aparición de un foco, realizar estudios para verificar la transmisión autóctona y la intensidad del evento. Además, se debe establecer la obligación legal de tomar medidas adicionales de control por los responsables de los proyectos que impliquen modificación ambiental, como la realización de estudios de evaluación del riesgo de transmisión, y acciones de mitigación del riesgo, detección temprana y tratamiento oportuno de los casos.","palabras_clave":"Leishmaniasis cutánea|Leishmaniasis visceral|Fenómenos ecológicos y ambientales|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6626,"idioma_id":4,"titulo":"Dietary whey reduces energy intake and alters hypothalamic gene expression in obese phyto-oestrogen-deprived male rats","resumen":"Removing dietary phyto-oestrogens in adult male rats causes obesity and diabetes. As whey proteins have been reported to reduce food intakeand improve glucose homoeostasis, we investigated whether they could attenuate susceptibility to obesity and diabetes due to phytooestrogendeprivation. To this end, thirty male Wistar rats were fed a high-phyto-oestrogen (HP) or a phyto-oestrogen-free (PF) diet for10 weeks; six rats from each group were killed. The remaining HP animals (six animals) continued receiving the HP diet for 6 weeks. Theremaining PF rats (twelve rats) were divided in two groups: one was given the PF diet and the other a variation of the PF diet plus wheyprotein (PF-W). Body weight, food intake and adipose tissue weights were recorded. Hypothalamic mRNA expressions of orexigenic(neuropeptide Y, agouti-related protein (AgRP)) and anorexigenic (pro-opiomelanocortin (POMC), cocaine-amphetamine-related transcript(CART)) neuropeptides were quantified by real-time PCR. Serum glucose, insulin and total thyroxine (T4), thyroid-stimulating hormone,testosterone and oestradiol were assessed. After 10 weeks of PF diet, increased body weight, adiposity and energy intake, with up-regulationof AgRP and down-regulation of POMC´, were observed. Longer treatment exacerbated these results, increased total T4 levels, reducedoestradiol levels and impaired glucose homoeostasis. PF-W reduced energy intake and increased POMC expression; however, body weightand adiposity remained unchanged. PF-W could not prevent the hormonal changes or the high circulating glucose levels induced by phytooestrogendeprivation, but reduced fasting insulin. These data demonstrate that, although 6 weeks of whey administration could not preventobesity in phyto-oestrogen-deprived rats, the reduction in energy intake and circulating insulin could be beneficial with longer treatments.","palabras_clave":"WHEY|OBESITY|HYPOTALAMUS|PHYTOESTROGENS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6627,"idioma_id":4,"titulo":"Bacterial nanocellulose production and application: a 10-year overview","resumen":"Production of bacterial nanocellulose (BNC) is becoming increasingly popular owing to its environmentally friendly properties. Based on this benefit of BNC production, researchers have also begun to examine the capacity for cellulose production through microbial hosts. Indeed, several research groups have developed processes for BNC production, and many studies have been published to date, with the goal of developing methods for large-scale production. During BNC bioproduction, the culture medium represents approximately 30 % of the total cost. Therefore, one important and challenging aspect of the fermentation process is identification of a new cost-effective culture medium that can facilitate the production of high yields within short periods of time, thereby improving BNC production and permitting application of BNC in the biotechnological, medical, pharmaceutical, and food industries. In this review, we addressed different aspects of BNC production, including types of fermentation processes and culture media, with the aim of demonstrating the importance of these parameters.","palabras_clave":"BACTERIAL NANOCELLULOSE |BIOMATERIAL|BIOPOLYMERS|BIOPROCESS|BIOPRODUCTS|FERMENTATION PROCESS|GLUCONACETOBACTER XYLINUS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6628,"idioma_id":5,"titulo":"Estudios para la puesta en valor de un edificio patrimonial con pinturas murales en zona sísmica: un caso de estudio","resumen":"En este trabajo se presenta una propuesta de intervención de unedificio de fines del siglo XIX, que alberga la obra pictórica delartista Fernando Fader, por lo que el valor patrimonial de los muralespintados en la casa-museo condiciona los trabajos de la puesta envalor. La metodología de estudio ha comprendido las siguientesetapas: relevamiento detallado, decisiones de emergencia, análisisde las condiciones de conservación, diagnóstico y propuesta derehabilitación. El relevamiento de las patologías comprueba eldaño sísmico por ausencia de arriostramientos y falta de capacidadportante de sus fundaciones. Desde el punto de vista de la seguridadse requieren restricciones al número de visitantes y después de lainspección de cielorrasos suspendidos, se clausuran salones deexposición. El análisis de condiciones de conservación requierede un estudios de suelos y fundaciones, cateos de materiales conidentificación de composición y medición de microvibracionesambientales para modelar el edificio por elementos finitos ycorroborar los estados de patologías encontradas. Durante elestudio se descubren pinturas ocultas del mismo artista, que sevalidan por difracción de rayos X. El diagnóstico indica que laconstrucción de mampostería monumental ha sufrido distintasintervenciones arquitectónicas a lo largo de su historia, derivandoen patologías que afectan su seguridad estructural frente a cargasverticales, ambientales y al sismo. En el análisis de la puesta en valorde la fundación se han considerado distintas alternativas disponibles,desde la no intervención hasta una intervención significativa, enfunción de la disponibilidad de acceso a la misma y de la tecnologíalocal. En las conclusiones se resalta el gran impacto tecnológico yeconómico de la rehabilitación de las fundaciones en relación a lasuperestructura metálica a incorporar y la necesidad de investigarmateriales compatibles para no afectar los murales.","palabras_clave":"COMPATIBILIDAD|MONITOREO|MURALES|SEGURIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6629,"idioma_id":4,"titulo":"sIgA, peroxidase and collagenase in saliva of smokers aggressive periodontal patients","resumen":"Periodontal diseases are chronic inflammatory entities characterized by infiltration of leukocytes, loss of connective tissue and alveolar bone resorption.1 Aggressive periodontitis (AgP) was redefined as a complex disease exhibiting microbial alteration and cellular dysfunction, which differs from chronic periodontal disease in the underlying molecular mechanisms of its pathogenesis.2 It comprises a heterogeneous group of periodontal diseases that affect adolescents and young adults. It is characterized by very rapid loss of periodontal tissue in otherwise clinically healthy subjects, and can be classified into localized (LAgP) and generalized forms (GAgP). Previous studies have investigated some factors that may increase host susceptibility to tissue destruction, including genetic factors, functional defects of polymorphonuclear (PMN) leukocytes and monocytes, and environmental risk factors. Cigarette smoking may be an important contributor to the development of periodontal disease. Evidence suggests that periodontitis is more prevalent in smokers than in non-smokers. Tobacco smoking is strongly associated with destructive periodontal disease, alveolar bone loss and poor response to periodontal therapy.3 This suggests that smoking might interfere with the host?s immune response. Tobacco metabolites suppress neutrophil function and inhibit the immune response. Nicotine has adverse effects on the role of PMN, altering the function of chemotaxis and phagocytosis and interfering with the production of immunoglobulins (Igs). Cigarette smoking can alter T-cell immune regulation and B-cell differentiation, generating a decrease in the production of Igs, which protect the oral mucosa against periodontal pathogenic bacteria.4 PMNs appear to play a pivotal role in aggressive periodontitis. Neutrophil granules are responsible for the neutrophil function and can be divided into three main types, according to their azurophil granule constituents. Myeloperoxidase is the most effective microbial and cytotoxic mechanism of leucocytes. Oral peroxidase is composed of two enzymes: salivary peroxidase, which contributes 80% of oral peroxidase, and myeloperoxidase which contributes the remaining 20%.5Damage to periodontal tissue is usually detected by means of periodontal probing, which shows loss of attachment of the tooth, or by radiographs that detect alveolar bone loss. These methods also evaluate the damage caused by previous destruction episodes, resulting in a retrospective diagnosis. Increasing attention is being given to saliva testing due to the need for a simple, non-invasive method for assessing drug intoxicated patients. Collecting saliva is easy, involves neither stress nor risk of injuries from needles, and patients can collect it themselves after instruction. Saliva contains a wide array of components that are very sensitive to toxic substances, and reflects a real-time level of biomarkers.6Some inflammatory mediators and other molecules originated from tissue destruction have been detected in gingivo-crevicular fluid (GCF) and saliva of patients with chronic periodontitis (ChP) or adult periodontitis.7-8  Results of our previous studies have shown significant statistical differences in certain GCF markers and whole saliva between patients with ChP and periodontally healthy subjects 9-10 and between patients with AgP and periodontally healthy individuals.11The aim of this study was to investigate the effect of smoking on total salivary sIgA, peroxidase and collagenase in patients with generalized aggressive periodontal disease and to investigate the relationships with their clinical parameters.","palabras_clave":"saliva|smokers|aggressive periodontitis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6630,"idioma_id":5,"titulo":"Metodología para el Tratamiento del Proceso de  Crowdsourcing en Aplicaciones Móviles Basadas en  Servicios de Geolocalización","resumen":"En este artículo se presenta una metodología para el tratamiento del proceso de Crowdsourcing en aplicaciones móviles.Trata diferentes cuestiones como la valoración de colaboraciones, definición de criterios para aprobar o rechazar una colaboración, determinación del nivel de experticia de usuarios, etc. Todas estas cuestiones se analizan agregando el parámetro de la geolocalización como parte influyente en las mismas dando por ejemplo más valor a una colaboración que se realice dentro del ámbito geográfico de incumbencia a una que se realiza posteriormente fuera de dicha locación. Además, se establecen una serie de pasos que se pueden seguir para diseñar en forma exitosa una aplicación de crowdsourcing geolocalizada, ofreciendo al diseñador un marco inicial sobre el cual establecer sus propios parámetros.","palabras_clave":"CROWSOURCING|GEOLOCALIZACION|APLICACIONES MOVILES|LSB (LOCATION BASED SERVICES)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6631,"idioma_id":4,"titulo":"Transcript profiling reveals that cysteine protease inhibitors are up-regulated in tuber sprouts after extended darkness","resumen":"Potato (Solanum tuberosum L.) tubers are an excellent staple food due to its high nutritional value. When the tuber reaches physiological competence, sprouting proceeds accompanied by changes at mRNA and protein levels. Potato tubers become a source of carbon and energy until sprouts are capable of independent growth. Transcript profiling of sprouts grown under continuous light or dark conditions was performed using the TIGR 10K EST Solanaceae microarray. The profiles analyzed show a core of highly expressed transcripts that are associated to the reactivation of growth. Under light conditions, the photosynthetic machinery was fully activated; the highest up-regulation was observed for the Rubisco activase (RCA), the glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the Photosystem II 22 kDa protein (CP22) genes, among others. On the other hand, sprouts exposed to continuous darkness elongate longer, and after extended darkness, synthesis of chloroplast components was repressed, the expression of proteases was reduced while genes encoding cysteine protease inhibitors (CPIs) and metallocarboxypeptidase inhibitors (MPIs) were strongly induced. Northern blot and RT-PCR analysis confirmed that MPI levels correlated with the length of the dark period; however, CPI expression was strong only after longer periods of darkness, suggesting a feedback loop (regulation mechanism) in response to dark-induced senescence. Prevention of cysteine protease activity in etiolated sprouts exposed to extended darkness could delay senescence until they emerge to light.","palabras_clave":"cysteine protease inhibitors|Solanum tuberosum L.|  darkness"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6632,"idioma_id":4,"titulo":"Real-Time Vessel and Oil Spill Detection using SAR Satellite Imagery: Application to the Argentine Ocean Littoral","resumen":"Oil-spill and furtive vessel detection are among the most required real-time monitoringtasks for environmental and government authorities. In Argentina, whose sea jurisdiction is huge (about one million square km), human asisted monitoring is unfeasible, and therefore remote sensing technologies, for instance satellite imagery, are the only operative and economically feasible solution. In this paper we present AMTO (Ambiente de Monitoreo Terrestre- Oceánico), a system for real-time vessel and oil spill detection using distribution parameter estimation as a local feature descriptor in the SAR imagery available through the Argentine SAOCOM and Italian COSMO satellite constellations. These results are incorporated in a software product that is being deployed at the National Aerospace Activities Council of Argentina (CONAE).","palabras_clave":"VESSEL DETECTION|SAR IMAGERY| OIL SPILL DETECTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6633,"idioma_id":5,"titulo":"ESPINALIOMA DE LENGUA: INFORME DE UN CASO CLÍNICO.","resumen":"La neoplasia maligna diagnosticada como carcinoma espinocelular, epidermoide o de células escamosas tiene su origen en las células escamosas del epitelio que recubre la mucosa, es una de las más prevalentes de la cavidad bucal, las zonas anatómicas más frecuentes son bordes de la lengua y el piso de boca. El caso clínico expuesto se trata en un individuo del sexo masculino, de 62 años de edad. El paciente sufre de hipertensión arterial y su glucemia elevada, presenta diabetes tipo 2, la lesión se inició en el borde de la lengua del lado izquierdo en el tercio posterior con sintomatología dolorosa hace aproximadamente 7 años.La lesión se ubica en la lengua cara dorsal, en el borde izquierdo en sus tres tercios. Se manifiesta como un tumor ligeramente redondeado, 5cm en su eje longitudinal mayor y 2,5 cm en su eje menor.","palabras_clave":"CANCER|ULCERA TRAUMATICA|AUTODETECCION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6634,"idioma_id":4,"titulo":"Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay","resumen":"We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and\/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles.","palabras_clave":"immunochromatographic|carbapenemases|OXA-48-like|OXA-163"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6635,"idioma_id":5,"titulo":"Las exportaciones vitivinícolas argentinas: una historia basada en episodios","resumen":"Este trabajo explora los diferentes episodios por los cuales transitó la industria vinícola argentina durante el periodo 1980-2012, en el marco de la globalización del sector. En particular, se estudia la importancia que tuvieron algunos bodegueros en dicho proceso así como el efecto que tuvo el tipo de cambio y los mercados receptores del vino argentino en la expansión de las exportaciones en los últimos años. La hipótesis de este trabajo es que, a partir de los años 80, los bodegueros vieron en las exportaciones una salida a la crisis más importante que atravesó el sector en la Argentina. Esto implicó, necesariamente, una transformación del mismo para producir vinos de calidad que pudieran ser vendidos en el mercado internacional. Así, los bodegueros sumaron a una tradición vitivinícola centenaria nuevas prácticas y estrategias de desarrollo. Por otro lado, a lo largo de 30 años, diversas políticas macroeconómicas influyeron en la expansión del sector en general y en las exportaciones en particular. En síntesis, en este trabajo intentamos demostrar que el crecimiento de las exportaciones de vinos argentinos tuvo un camino sinuoso y diferente a lo que la bibliografía hasta el momento ha encontrado para los países exportadores del ?nuevo mundo?. El trabajo se basa en diferentes fuentes de información cuantitativas brindadas por el Instituto Nacional de Vitivinicultura (INV), en las estadísticas de la Bolsa de Comercio de Mendoza y la Organización Internacional de la Viña y el Vino (OIV), así como en entrevistas recogidas en fuentes secundarias a informantes claves del sector.Palabras-claves: vitivinicultura, mercado de vino, globalización, Argentina.","palabras_clave":"VITIVINICULTURA|MERCADO DE VINO|GLOBALIZACION|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6636,"idioma_id":4,"titulo":"Towards efficient biocatalysts: photo-immobilization of a lipase on a novel lysozyme amyloid-like nanofibrils","resumen":"Herein, we report the preparation and characterization of a new biocatalyst based on the photo- immobilization of a lipase onto hybrid protein : sugar nanofibrils. The nanofibrils are obtained through the aggregation of hen white egg lysozyme induced by the highly sulfated glycosaminoglycan heparin. The new hybrid nanomaterial could be easily functionalized using photochemical reaction to attach lipases through dityrosine covalent bonds. Compared to the free enzyme, the photo-immobilized lipase has better thermostability and enhanced resistance to non-conventional environment. Structural and morphological characterization of the nanofibrils shows that they are compatible with amyloid-like aggregates. In addition, the supramolecular arrangement of heparin and lysozyme within the building unit of the nanofibrils is also proposed. The procedure reported herein could be useful to design a new generation of insoluble biocatalyst by a single photo-click step which is definitely cleaner and faster than conventional chemical cross-linked procedures.","palabras_clave":"Nanocatalizadores|Nanotubos biológicos|Amiloides|Biodiesel"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6637,"idioma_id":4,"titulo":"Biotechnological design to optimize the production of bioactive Lactobacillus plantarum by-products: Novel chronic wound treatment","resumen":"Lactobacillus plantarum culture supernatants (LAPS) have antimicrobial, pro?]healing and anesthetic properties, so our medical team applied whole cultures and LAPS in chronic wounds with encouraging results. Metabolites responsible for LAPS properties and its action mechanism were previously determined. Objective: to design methods and culture media to increase the production of LAPS metabolites and optimize it?Ls therapeutic effectiveness. Modifications in MRS broth composition were made and L. plantarum was cultivated in the resulting different media with different physicochemical conditions to obtain modified supernatants (LAPSm). Antimicrobial and anti-biofilm activity (crystal violet) of LAPSm on strains typically isolated from chronic wounds (Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis and Serratiamarcescens), was tested. Concentration of barbiturates in LAPSm was quantified by GCMS and its relative anesthetic potency in healthy volunteers was evaluated. DNAase and auto inducers type 2biological activity in LAPSm were quantified. In addition, a safety assessment of LAPSm application on healthy skin of 200 volunteers was performed. LAPSm obtained from media with high concentrations of yeast extract possessed the greatest anesthetic power. Those containing greater amount of proteins, cations and surfactants had the highest capacity of biofilm disruption (p&lt;0.01). When glucose and galactose concentration were increased in media, LAPSm had the greatest antimicrobial power (p&lt;0.05). Finally, there is great safety in the application of LAPSm as no edema or erythema was observed. The results obtained will allow the manufacture of LAPSm with greater therapeutic effectiveness and even custom properties for each type of wound. ","palabras_clave":"HERIDAS CRONICAS|LACTOBACILLUS PLANTARUM|SOBRENADANTES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6638,"idioma_id":4,"titulo":"?Target Tracking System Using Multiple Cameras and Bayesian Estimation?","resumen":"Abstract? In recent years, the increase in amount of camera networks available has led to the rise up of new techniques and applications of signal processing. In this work, an implementation of image sensor network, cameras, for mobile target tracking is shown. More specifically, it is performed an application of particlefilter in a distributed way, based on leader agents for state estimation. Finally, simulations are presented, as well as a real experiment made with a camera network to track a mobile platform.","palabras_clave":"Target tracking|Particle filter|Camera network"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6639,"idioma_id":5,"titulo":"Aportes de las ilusiones ópticas a diferentes campos del conocimiento","resumen":"Una ilusión, vinculada a los sentidos,es una distorsión de la percepción. Puede ser óptica, olfativa, auditiva,gustativa o táctil.Las ilusiones ópticas llevan a percibirla realidad de manera distorsionada a través de la vista. Pueden ser decarácter fisiológico, asociadas a los efectos de una estimulación de los ojos oel cerebro; o cognitivo, en la que interviene nuestro conocimiento del mundo.No están sometidas a la voluntad ypueden variar entre las personas, y dependen, entre otros factores, de laagudeza y el campo visual, el daltonismo y el astigmatismo. Entender estos fenómenos es útil paracomprender las limitaciones del sentido visual del ser humano y la posibilidadde distorsión, ya sea en lo relativo a la forma, el color, la dimensión y laperspectiva de lo observado. Muchos artistas han aprovechado lasilusiones ópticas para dar a sus obras un aspecto mágico, de profundidad,ambigüedad y contraste. En este trabajo se presentan algunos tipos de ilusionesópticas y se muestra su aporte a diferentes ámbitos del conocimiento. ","palabras_clave":"ILUSIONES ÓPTICAS|PINTURA |ESCULTURA|ADMINISTRACIÓN|ONTOLOGÍA DEL LENGUAJE|CAMBIO DE PARADIGMA|PERCEPCIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6640,"idioma_id":5,"titulo":"?La Vivencia como Unidad de Análisis de la Conciencia: Sentidos y Significados en Trayectorias de Profesionalización de Psicólogos y Profesores en Comunidades de Práctica?","resumen":"RESUMEN.Eltrabajo explora la potencialidad de escritos de Vygotsky sobre el concepto de pereshivanie ? vivencia -, entrelazandoemoción-cognición y sujeto-situación-historia-contexto, como lo hace el autor enPensamiento y Lenguaje (1934).Profundiza la significatividad de las unidades de análisis sistémicas,genéticas y dialécticas para abordar el desarrollo, la conciencia y la educación.Retomamos la propuesta de Valsiner de pensar la síntesis dialéctica mediante preguntas: ¿Es la emoción, en sutensión entre contrarios, obstáculo para la cognición, como en el?cortocircuito?? ¿O la emoción, en su aporte a la articulación de significado ysentido, experiencia y concepto, puede anticipar lo nuevo, lo emergente? Siambas posibilidades existen, ¿de qué depende la posición que ocupa el afecto enla conciencia? Abrimos un diálogo de conceptos y problematizaciones entrelíneas de investigación, entramados con reflexiones y análisis de experienciasformativas en trayectorias de profesionalización de psicólogos, docentes yprofesores de psicología en escenarios educativos.&nbsp;","palabras_clave":"SÍNTESIS|DIALÉCTICA|CORTOCIRCUITO|CONCIENCIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6641,"idioma_id":4,"titulo":"Usnic Acid Activity on Oxidative and Nitrosative Stress of Azole-Resistant Candida albicans Biofilm","resumen":"AbstractSeveral studies report about that (+)-usnic acid (UA), a secondary metabolite produced by lichens, inhibits growth of different bacteria, and fungi; however, mechanism of its antimicrobial activity remained unknown.In the present study, we have explored the ability of UA, obtained from Usnea amblyoclada, as an antibiofilm agent again stazole-resistant(RCa) and azole-sensitive (SCa) Candida albicansstrains by studying the cellular stress and antioxidant response in the biofilms. The biofilm inhibitory concentration (BIC) of UA (4 µg\/mL) exhibited significant biofilm inhibition, 71.08% for RCa and 87.84% for SCa. Confocal scanning laser microscopy (CSLM) showed that in presence of UA the morphology of the mature biofilm was altered (reduced the biomass and thickness).The antifungal effect was mediated by an oxidative and nitrosative stress, with a significant accumulation of intracellular and extracellular ROS detected by CSLMand by nitro blue tetrazolium, respectively. Non-enzymatic and enzymatic antioxidant defenses increased in both strains compared to those of the biofilm basal levels as response to the increase of oxidant metabolites. The present study shows, for the first time, that UA can alter the prooxidant-antioxidant balance, which may be the cause of the irreversible cell damage and lead to cell death. Our results suggest that UA could be an alternative for the Candida infections treatment, which deserves further investigation.","palabras_clave":"Usnic acid|Candida albican|Biofilms|prooxidant-antioxidant balance"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6642,"idioma_id":4,"titulo":"Oxidative stress generation of silver nanoparticles in three bacterial genera and its relationship with the antimicrobial activity","resumen":"Oxidative stress is a condition caused by the high intracellular concentrations of reactive oxygen species (ROS) that includes superoxide anion radicals, hydroxyl radicals and hydrogen peroxide. Nanoparticles could cause rapid generation of free radicals by redox reactions. ROS can react directly with membrane lipids, proteins and DNA and are normally scavenged by antioxidants that are capable of neutralizing; however, elevated concentrations of ROS in bacterial cells can result in oxidative stress. The aim of this work was contribute to the knowledge of action mechanism of silver nanoparticles (Ag-NPs) and their relation to the generation of oxidative stress in bacteria. We demonstrated that Ag-NPs generated oxidative stress in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa mediated by the increment of ROS and this increase correlated with a better antimicrobial activity. On the other hand, we showed that the oxidative stress caused by the Ag-NPs biosynthesized was associated to a variation in the level of reactive nitrogen intermediates (RNI). Oxidative stress in bacteria can result from disruption of the electronic transport chain due to the high affinity of Ag-NPs for the cell membrane. The formation of ROS and RNI by Ag-NPs may also be considered to explain the bacterial death.","palabras_clave":"Oxidative stress |Silvernanoparticles |Biosynthesis |Antimicrobialactivity"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6643,"idioma_id":5,"titulo":"Desempeño en funciones ejecutivas y síntomas comórbidos asociados en niños con Trastorno por déficit de atención con hiperactividad (TDAH)","resumen":"El objetivo del presente estudio fue analizar el desempeño de las funciones ejecutivas en niños con Trastorno por déficit de atención con hiperactividad en relación con síntomas comórbidos asociados, internalizantes y externalizantes. Se evaluaron 45 niños de 8 a 14 años con diagnóstico de TDAH con pruebas de funciones ejecutivas. Los resultados evidenciaron que el grupo de niños con síntomas internalizantes presentaron un desempeño inferior en organización y planificación y en inhibición motora, y el grupo de niños con síntomas externalizantes un desempeño inferior en inhibición motora, en comparación con los niños sin síntomas comórbidos, lo cual indicaría que las variables analizadas permitirían colaborar con el proceso de evaluación diagnóstica del trastorno. Describir el desempeño ejecutivo del trastorno en función de diversas asociaciones comórbidas, posibilitaría un mejor conocimiento de la entidad y un adecuado diagnóstico diferencial.","palabras_clave":"Comorbilidades|Funciones Ejecutivas|Niños|TDAH"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6644,"idioma_id":4,"titulo":"Creating resilient water governance for irrigated producers in Mendoza, Argentina","resumen":"This research explores the institutional water governance system of irrigated agricultural producers inMendoza, Argentina in the context of a changing climate (predominantly increasing events of drought and water scarcity). An assessment is made of the impact of water governance instruments on producers using the methodology of vulnerability and adaptive capacity. Analysis focuses on the impact of the institutional water governance system on the adaptive capacity of producers? resources, or capitals(human, social, economic, technological, and natural). Conclusions and suggestions for improving the resiliency of agricultural producers and increasing the adaptive dimensions of Mendoza´s water governance system are made based on this analysis.","palabras_clave":"WATER GOVERNANCE|CLIMATE ADAPTATION|PRODUCER CAPITALS|INHERENCE WATER INSTRUMENT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6645,"idioma_id":4,"titulo":"CONSEQUENCES OF EXCESSIVE PLASTICITY IN THE HIPPOCAMPUS INDUCED BY PERINATAL ASPHYXIA","resumen":"Perinatal asphyxia (PA) is one of the most frequent risk factors for severalneurodevelopmental disorders (NDDs) of presumed multifactorial etiology. Dysfunction of neuronal connectivity is thought to play a central role in the pathophysiology of NDDs.Because underlying causes of some NDDs begin before\/during birth, we asked whether this clinical condition might affect accurate establishment of neural circuits in the hippocampus as a consequence of disturbed brain plasticity. We used a murine model that mimics the pathophysiological processes of perinatal asphyxia. Histological analyses of neurons (NeuN), dendrites (MAP-2), neurofilaments (NF-M\/Hp) and correlative electron microscopy studies of dendritic spines were performed in Stratum radiatum of the hippocampal CA1 area after postnatal ontogenesis. Protein and mRNA analyses were achieved by Western blot and RT-qPCR. Behavioral tests were also carried out. NeuN abnormal staining and spine density were increased. RT-qPCR assays revealed a &#946;-actinmRNA over-expression, while Western blot analysis showed higher &#946;-actin protein levels in synaptosomal fractions in experimental group. M6a expression, protein involved in filopodium formation and synaptogenesis, was also increased. Furthermore, we found that PI3K\/Akt\/GSK3 pathway signaling, which is involved in synaptogenesis, was activated. Moreover, asphyctic animals showed habituation memory changes in the open field test.Our results suggest that abnormal synaptogenesis induced by PA as a consequence of excessive brain plasticity during brain development may contribute to the etiology of the NDDs. Consequences of this altered synaptic maturation can underlie some of the later behavioral deficits observed in NDDs.","palabras_clave":"Perinatal Asphyxia|Plasticity|Hippocampus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6646,"idioma_id":4,"titulo":"AN ALTERNATIVE HYBRID EVOLUTIONARY TECHNIQUE FOCUSED ON ALLOCATING MACHINES AND SEQUENCING OPERATIONS","resumen":"We present here a hybrid algorithm for the Flexible Job-Shop Scheduling Problem (FJSSP). This problem involves the optimal use of resources in a flexible production environment in which each operation can be carried out by more than a single machine. Our algorithm allocates, in a first step, the machines to operations and in a second stage it sequences them by integrating a Multi-Objective Evolutionary Algorithm (MOEA) and a path-dependent search algorithm (Multi-Objective Simulated Annealing), which is enacted at the genetic phase of the procedure. The joint interaction of those two components yields a very efficient procedure for solving the FJSSP. An important step in the development of the algorithm was the selection of the right MOEA. Candidates were tested on problems of low, medium and high complexity. Further analyses showed the relevance of the search algorithm in the hybrid structure. Finally, comparisons with other algorithms in the literature indicate that the performance of our alternative is good.","palabras_clave":"FLEXIBLE JOB-SHOP SCHEDULING PROBLEM|OPTIMIZATION|MULTI-OBJECTIVE HYBRID EVOLUTIONARY ALGORITHM|PRODUCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6647,"idioma_id":4,"titulo":"Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch","resumen":"Background.Myocardial stretch increases force biphasically: the Frank?Starling mechanismfollowed by the slow force response (SFR). Based on pharmacological strategies,we proposed that epidermal growth factor receptor (EGFR) activation is crucialfor the SFR development. Pharmacological inhibitors could block ErbB4, a familymember present in the adult heart.&nbsp; Weaimed to specifically test the role of EGFR activation after stretch, with aninterference RNA incorporated into a lentiviral vector (shRNA-EGFR). Methods and Results.Silencing capability of p-shEGFR was assessed on EGFR-GFP transientlytransfected HEK293T cells. Four weeks after lentivirus injection into Wistarrat left ventricular wall, shRNA-EGFR-injected hearts showed ~60% reduction ofEGFR protein expression compared to shRNA-SCR. ErbB2 and ErbB4 expression didnot change. The SFR to stretch evaluated in isolated papillary muscles was~130% of initial rapid phase in shRNA-SCR group, while it was blunted inshRNA-EGFR-expressing muscles. Ang II-dependent NHE1 activation was indirectlyevaluated by pHi measurements in bicarbonate-free medium, evidencingan increase in shRNA-SCR injected myocardium, effect not observed in thesilenced group. Ang II or EGF-triggered ROS production was significantlyreduced in shRNA-EGFR-injected hearts compared to shRNA-SCR. Chronic lentivirustreatment neither affected myocardial basal redox state (T-BARS) norNADPH-oxidase activity or expression. Finally, Ang II or EGF triggered redoxsensitive pathway leading to p90RSK activation in shRNA-SCR-injectedmyocardium, effect that was absent in the shRNA-EGFR group.Conclusions.Our results provide evidence that specific EGFR activation after myocardialstretch is a key factor to promote redox-sensitive kinase activation pathwayleading to SFR development.","palabras_clave":"EGFR|heart|siRNA|Stretch"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6648,"idioma_id":5,"titulo":"Primer registro de Liolaemus martorii Abdala, 2003 (Iguania: Liolaemidae) en la provincia de Buenos Aires (Argentina)","resumen":"Se reporta el primer registro de Liolaemus martorii en la provincia de Buenos Aires, específicamente en la Villa Balnearia 7 de Marzo (Paraje La Baliza), Partido de Patagones. Se detallan las características del lugar de colecta y se remarca la importancia que tiene este nuevo registro con respecto a la distribución actual conocida para la especie en Argentina. Hasta el momento, la distribución conocida de esta especie ha sido limitada a la provincia de Río Negro, donde los ejemplares fueron colectados al sur del río homónimo en la localidad de las Grutas, en la Reserva de usos múltiples Caleta de los Loros y en el balneario La Lobería situada 30 Km hacia el sur de Viedma (Abdala 2003, Scrocchi et al. 2010). Este nuevo registro de colección corresponde a un macho adulto de L. martorii que fue capturado entre las 12 y las 13 hs, en un área con sustrato arenoso situado a escasos metros de la margen norte del Río Negro.","palabras_clave":"Liolaemus martorii|Provincia de Buenos Aires|Distribución"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6650,"idioma_id":4,"titulo":"Activity patterns of St. Louis encephalitis and West Nile viruses in free ranging birds during a human encephalitis outbreak in Argentina","resumen":"St. Louis encephalitis virus (SLEV) (Flavivirus) is a reemerging arbovirus in the southern cone of South America. In 2005, an outbreak of SLEV in central Argentina resulted in 47 human cases with 9 deaths. In Argentina, the ecology of SLEV is poorly understood. Because certain birds are the primary amplifiers in North America, we hypothesized that birds amplify SLEV in Argentina as well. We compared avian SLEV seroprevalence in a variety of ecosystems in and around Córdoba city from 2004 (before the epidemic) and 2005 (during the epidemic). We also explored spatial patterns to better understand the local ecology of SLEV transmission. Because West Nile virus (WNV) was also detected in Argentina in 2005, all analyses were also conducted for WNV. A total of 980 birds were sampled for detection of SLEV and WNV neutralizing antibodies. SLEV seroprevalence in birds increased 11-fold from 2004 to 2005. Our study demonstrated that a high proportion (99.3%) of local birds were susceptible to SLEV infection immediately prior to the 2005 outbreak, indicating that the vertebrate host population was primed to amplify SLEV. SLEV was found distributed in a variety of environments throughout the city of Córdoba. However, the force of viral transmission varied among sites. Fine scale differences in populations of vectors and vertebrate hosts would explain this variation. In summary, we showed that in 2005, both SLEV and to a lesser extent WNV circulated in the avian population. Eared Dove, Picui Ground-Dove and Great Kiskadee are strong candidates to amplify SLEV because of their exposure to the pathogen at the population level, and their widespread abundance. For the same reasons, Rufous Hornero may be an important maintenance host for WNV in central Argentina. Competence studies and vector feeding studies are needed to confirm these relationships.","palabras_clave":"BIRDS|WEST NILE VIRUS|ARGENTINA|ANTIBODIES|MOSQUITOES|ENCEPHALITIS|DISEASES ECOLOGY|VERTEBRATES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6651,"idioma_id":4,"titulo":"Distribution of the members of the Pipiens Assemblage in the sympatric area from Argentina: which is where and when?","resumen":"Given their medical and veterinary relevance, the members of the Pipiens Assemblageare a worldwide target of ecological research. In Buenos Aires, Argentina, the Thedistribution of Culex pipiens s.s. and Cx. quinquefasciatus converge in Buenos Aires,Argentina,, and where hybrids have been detected. Each member of the assemblageexhibits a distinct eco-physiological behaviour that can affect their its efficiency inpathogen transmission. This research aimed Our aim was to identify the environmentaldrivers for the spatio-temporal distribution of each member, focusing on latitudinal andurbanization gradients. AImmatures of mosquitoes were surveyed in artificial containersfound within 11 public cemeteries were surveyed for immatures of mosquitoes, whichwere raised until up to the adult stage and identified by their male genitalia. Thedistribution of each member was associated with the environment in a GeneralizedLinear Model. The variable accounting for most of the heterogeneity was latitude; Cx.quinquefasciatus was collected more frequently at northern latitudescemeteries, whereasCx. pipiens and hybrids were more likely at the southern extreme. The urbanizationgradient was also important, associated with the occurrence of Cx. quinquefasciatus andhybrids at the high and low end, respectively. Other relevant variables were cemeterytotal area, the proportion with graves and the presence of plastic flowers in thecontainers. The information presented herein provides essential baseline data forsurveillance programs and control activities.","palabras_clave":"CULEX|PIPIENS|DISTRIBUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6652,"idioma_id":4,"titulo":"Fluctuations of a surface relaxation model in interacting scale free networks","resumen":"Isolated complex networks have been studied deeply in the last decades due to the fact that many real systems can be modeled using these types of structures. However, it is well known that the behavior of a system not only depends on itself, but usually also depends on the dynamics of other structures. For this reason, interacting complex networks and the processes developed on them have been the focus of study of many researches in thelast years. One of the most studied subjects in this type of structures is the Synchronization problem, which is important in a wide variety of processes in real systems. In this manuscript we study the synchronization of two interacting scale-free networks, in which each node has ke dependency links with different nodes in the other network. We map the synchronization problem with an interface growth, by studying the fluctuations in the steady state of a scalar field defined in both networks.We find that as ke slightly increases from ke = 0, there is a really significant decreasing in the fluctuations of the system. However, this considerable improvement takes place mainly for small values of ke, when the interaction between networks becomes stronger there is only a slight change in the fluctuations. We characterize how the dispersion of the scalar field depends on the internal degree, and we show that a combination between the decreasing of this dispersion and the integer nature of our growth model are the responsible for the behavior of the fluctuations with ke.","palabras_clave":"Synchronization|Complex networks|Multiplex networks"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6653,"idioma_id":4,"titulo":"The Water to Solute Permeability Ratio Governs the Osmotic Volume Dynamics in Beetroot Vacuoles","resumen":"Plant cell vacuoles occupy up to 90% of the cell volume and, beyond their physiological function, are constantly subjected to water and solute exchange. The osmotic flow and vacuole volume dynamics relies on the vacuole membrane -the tonoplast- and its capacity to regulate its permeability to both water and solutes. The osmotic permeability coefficient (Pf ) is the parameter that better characterizes the water transport when submitted to an osmotic gradient. Usually, Pf determinations are made in vitro from the initial rate of volume change, when a fast (almost instantaneous) osmolality change occurs. When aquaporins are present, it is accepted that initial volume changes are only due to water movements. However, in living cells osmotic changes are not necessarily abrupt but gradually imposed. Under these conditions, water flux might not be the only relevant driving force shaping the vacuole volume response. In this study, we quantitatively investigated volume dynamics of isolated Beta vulgaris root vacuoles under progressively applied osmotic gradients at different pH, a condition that modifies the tonoplast Pf . We followed the vacuole volume changes while simultaneously determining the external osmolality time-courses and analyzing these data with mathematical modeling. Our findings indicate that vacuole volume changes, under progressively applied osmotic gradients, would not depend on the membrane elastic properties, nor on the non-osmotic volume of the vacuole, but on water and solute fluxes across the tonoplast. We found that the volume of the vacuole at the steady state is determined by the ratio of water to solute permeabilites (Pf \/Ps ), which in turn is ruled by pH. The dependence of the permeability ratio on pH can be interpreted in terms of the degree of aquaporin inhibition and the consequently solute transport modulation. This is relevant in many plant organs such as root, leaves, cotyledons, or stems that perform extensive rhythmic growth movements, which very likely involve considerable cell volume changes within seconds to hours.","palabras_clave":"Vacuole|Red beet|Beta Vulgaris|Water flux|Solute flux|Gradual gradients|Mathematical modelling|Simulation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6654,"idioma_id":5,"titulo":"Campo académico del turismo: articulaciones docencia - investigación.","resumen":"El proyecto: ?De las articulaciones entre el subcampo profesional y el subcampo de investigación al interior del campo académico de las carreras de Licenciatura en Turismo del CONDET?, es un estudio que centrado en las temáticas de Desarrollo Local y Sustentabilidad, analizó de -modo relacional- los contenidos desplegados en: Planes de estudio, Programas de asignaturas y publicaciones que daban cuenta de proyectos de investigación (período 2001-2012). La selección de los ejes mencionados se debe a ser éstos los ?paradigmas? más referenciados en las publicaciones producidas por los docentes-investigadores de este campo en la Argentina.Este artículo completa las conclusiones arribadas a partir de la finalización del proyecto. La primera parte ya ha sido publicada  y refería a las articulaciones entre los contenidos de los Planes de Estudios y los Programas de las asignaturas. En esta oportunidad se analizan las articulaciones entre producción, transmisión y transferencia de conocimientos.","palabras_clave":"ARTICULACIONES|DOCENCIA|INVESTIGACIÓN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6655,"idioma_id":4,"titulo":"Altered levels of AtHSCB disrupts iron translocation from roots to shoots","resumen":"Plants overexpressing AtHSCB and hscb knockdown mutants showed altered iron homeostasis. The overexpression of AtHSCB led to activation of the iron uptake system and iron accumulation in roots without concomitant transport to shoots, resulting in reduced iron content in the aerial parts of plants. By contrast, hscb knockdown mutants presented the opposite phenotype, with iron accumulation in shoots despite the reduced levels of iron uptake in roots. AtHSCB play a key role in iron metabolism, probably taking part in the control of iron translocation from roots to shoots. Many aspects of plant iron metabolism remain obscure. The most known and studied homeostatic mechanism is the control of iron uptake in the roots by shoots. Nevertheless, this mechanism likely involves various unknown sensors and unidentified signals sent from one tissue to another which need to be identified. Here, we characterized Arabidopsis thaliana plants overexpressing AtHSCB, encoding a mitochondrial cochaperone involved in [Fe-S] cluster biosynthesis, and hscb knockdown mutants, which exhibit altered shoot\/root Fe partitioning. Overexpression of AtHSCB induced an increase in root iron uptake and content along with iron deficiency in shoots. Conversely, hscb knockdown mutants exhibited increased iron accumulation in shoots and reduced iron uptake in roots. Different experiments, including foliar iron application, citrate supplementation and iron deficiency treatment, indicate that the shoot-directed control of iron uptake in roots functions properly in these lines, implying that [Fe-S] clusters are not involved in this regulatory mechanism. The most likely explanation is that both lines have altered Fe transport from roots to shoots. This could be consistent with a defect in a homeostatic mechanism operating at the root-to-shoot translocation level, which would be independent of the shoot control over root iron deficiency responses. In summary, the phenotypes of these plants indicate that AtHSCB plays a role in iron metabolism.","palabras_clave":"IRON|ARABIDOPSIS THALIANA|IRON PARTITIONING|HCSB"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6656,"idioma_id":4,"titulo":"Model Order Reduction in computational multiscale fracture mechanics","resumen":"Nowadays, the model order reduction techniques have become an intensive research field because of the increasing interest in the computational modeling of complex phenomena in multiphysic problems, and its consequent increment in high-computing demanding processes; it is well known that the availability of high-performance computing capacity is, in most of cases limited, therefore, the model order reduction becomes a novelty tool to overcome this paradigm, that represents an immediately challenge in our research community. In computational multiscale modeling for instance, in order to study the interaction between components, a different numerical model has to be solved in each scale, this feature increases radically the computational cost. We present a reduced model based on a multi-scale framework for numerical modeling of the structural failure of heterogeneous quasibrittle materials using the Strong Discontinuity Approach (CSD). The model is assessed by application to cementitious materials. The Proper Orthogonal Decomposition (POD) and the Reduced Order Integration Cubature are the proposed techniques to develop the reduced model, these two techniques work together to reduce both, the complexity and computational time of the high-fidelity model, in our case the FE2 standard model.","palabras_clave":"COMPUTATIONAL MULTI-SCALE MODELING|CONTINUUM STRONG DISCONTINUITUY APPROACH|FRACTURE|MODEL ORDER REDUCTION|REDUCED-ORDER CUBATURE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6657,"idioma_id":5,"titulo":"Las políticas públicas y su visión de la Economía Social y Solidaria en Argentina","resumen":"A nivel internacional, la Economía Social y Solidaria (ESyS) ha cobrado relevancia en las últimas décadas por su potencialidad para contribuir a la generación de empleo, la inclusión social y el desarrollo de sus comunidades. Su capacidad de favorecer estos objetivos, sin embargo, se encuentra condicionada por la visión que posee el Estado nacional de la ESyS y el papel que las políticas públicas le asignan en la estrategia de lucha contra el desempleo y la exclusión social. En este marco, el artículo presenta los resultados de una investigación sobre las políticas públicas nacionales que incorporan referencias a la ESyS, vigentes en Argentina durante el periodo 2003-2013. La hipótesis de esta investigación plantea que las políticas públicas bajo análisis poseen una visión instrumental de la ESyS en la medida que la incorporan por su contribución coyuntural a la creación de empleo e ingresos para la población excluida. Los resultados de la investigación corroboran esta hipótesis y muestran cómo este enfoque restringe, por un lado, la efectividad de las políticas públicas de incidir de manera estructural en las condiciones socioeconómicas de las personas beneficiarias y, por otra parte, la capacidad de la ESyS de impulsar procesos de transformación sociopolítica.","palabras_clave":"ECONOMÍA SOCIAL Y SOLIDARIA|POLÍTICAS PÚBLICAS|EXCLUSIÓN SOCIAL|DESEMPLEO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6658,"idioma_id":4,"titulo":"Positional Isotope Exchange in  HX$\\cdot$(H$_2$O)$_n$ (X=F, I)  Clusters at Low Temperatures","resumen":"We present Molecular Dynamics simulation results describingproton\/deuteron exchange equilibria along hydrogen bonds atthe vicinity of HX acids (X = F, I) in aqueous clustersat low temperatures.To allow for an adequate description of proton transfer processes,our simulation scheme resorted on the implementation of aMultistate Empirical Valence Bond Hamiltonian coupled to aPath Integral scheme to account for effects derived from&nbsp; nuclearquantum fluctuations.We focused attention on clusters comprising a number of water moleculesclose to the threshold values necessaryto stabilize contact-ion-pairs. For X=F, our resultsreveal a clear propensity of the heavy isotope to lie at thebond bridging the halide to the nearest water molecule. Contrasting, for X=I, the thermodynamicstability is reversed and the former connectivity is preferentially articulated viathe light isotope. These trends remain valid for undissociatedand ionic descriptions of the stable valence bond states.The preferences are rationalized in terms of differences in the quantumkinetic energies of the isotopes which, in turn, reflect the&nbsp; extent of thelocal spatial confinements prevailing along the different hydrogen bonds inthe clusters. In most cases, these features are also clearly reflectedin the characteristics of the corresponding stretching bands of the simulatedinfrared spectra. This opens interesting possibilitiesto gauge the extent of the isotopic thermodynamic stabilizations and the strengths of thedifferent hydrogen bonds byfollowing the magnitudes and shifts of the spectral signals in temperature controlledexperiments, performed on mixed clusters combining H$_2$O and HOD.","palabras_clave":"Procesos cuanticos nucleares|nanoclusters|simulacion computacional"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6659,"idioma_id":4,"titulo":"Electrophysiology for biomedical engineering students: a practical and theoretical course in animal electrocorticography","resumen":"The major challenge in laboratory teaching is the application of abstract concepts in simple and direct practical lessons. However, students rarely have the opportunity to participate in a laboratory that combines the practical learning with a realistic research experience. In Biomedical Engineering Career we offer short and optional courses as complement studies for students that initiate their Graduation Project. The objective of these theoretical and practical courses is to introduce them in the topics of their projects. The present work describes a practice in Electrophysiology to teach undergraduate students how to extract cortical information by using electrocorticographic technique. The students participate actively in some parts of the experience and then process and analyze the data obtained with different signal processing tools.","palabras_clave":"Education|Electrocorticography|Processing|Biomedical students"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6660,"idioma_id":5,"titulo":"La formalización de los trabajadores por cuenta propia en Argentina: análisis del programa Monotributo Social y de los factores que condicionan su alcance","resumen":"La informalidad es un fenómeno persistente en las economías de América Latina, con gran incidencia en los trabajadores por cuenta propia más pobres. Los programas públicos que fomentan la creación de emprendimientos entre la población vulnerable tienen como principal objetivo la creación de empleo y la inclusión social a través del trabajo. Un diseño de política pública coherente con este objetivo plantea la necesidad de desarrollar programas que faciliten la formalización ?y el acceso a sus beneficios- de los trabajadores por cuenta propia vulnerables. Con este objetivo, el Estado nacional argentino ha implementado desde el 2004 un régimen especial de tributación denominado Monotributo Social. Este trabajo identifica los principales desafíos que ha encontrado este programa en su formulación e implementación desde su creación hasta la actualidad y las estrategias que ha adoptado para abordarlos. Adicionalmente, indaga en los límites que enfrenta el programa para llegar a la población destinataria como resultado de su propia formulación, pero también debido a las condiciones que caracterizan a estos trabajadores.","palabras_clave":"INFORMALIDAD|POLÍTICAS PÚBLICAS|TRABAJO POR CUENTA PROPIA |VULNERABILIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6661,"idioma_id":4,"titulo":"Linear Decoder Analysis Applied to Multineuronal Records of Retinal Ganglion Cells","resumen":"Achieving a thorough understanding about the neural code is one of the neuroscientists? greatest challenges. One way to evaluate our current knowledge about the neural code is trying to reconstruct sensorial stimuli from neuronal responses. In spite of nowadays there are several types of decoding techniques, few of them have been used and analyzed in actual multi neuronal records of retinal ganglion cells. In this work, we had employed and analyzed a linear decoder to reconstruct different visual stimulus, white noise scramble and natural image, from multi neuronal records of retinal ganglion cells. The efficiency of the reconstruction depends on two factors: one the one hand, the type of visual stimulus, if parameters used were calculated since white noise, scramble or natural image , and on the other hand, the numbers of cells employed in the reconstruction.","palabras_clave":"Retina|Population code|Mutual Information|Neural reconstruction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6662,"idioma_id":4,"titulo":"Evaluation of the effectiveness of potential biocontrol yeasts against black sur rot and ochratoxin A occurring under greenhouse and field grape production conditions","resumen":"The efficacy of two strains of Lanchancea thermotolerans in preventing the growth and ochratoxin A (OTA) accumulation of ochratoxigenic fungi under greenhouse and field conditions were evaluated during three consecutive year trials. The data from this study showed that both yeast strains were able to control Aspergillus section Nigri species ochratoxin A accumulation in wine grapes at harvest stage. The inhibitory effects were dependent on the ochratoxigenic species, yeast strains, and year analyzed. Over all conditions assayed, ochratoxin A accumulation was reduced from 27% to 100%, depending on the conditions evaluated. These results are promising for future development of a bio-pesticide.","palabras_clave":"BIOCONTROL|YEAST|FIELD|FUNGI"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6663,"idioma_id":4,"titulo":"S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor","resumen":"Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope; among other characteristics S-layer from Lactobacillus acidophilus  binds to the cellular receptor DC-sign, which is involved in adhesion and infection of several families of bacteria.In this report beside the S-layer from Lb. acidophilus we evaluated the activity of three new S-layer proteins from the lactobacillus family (Lb. brevis, Lb. helveticus and Lb.kefiri) over the infection of representative microorganisms important in human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models . We discovered that pre-treatment of the bacteria before infection with S-layers from Lb. acidophilus and Lb.brevis reduced both viability and infection of the pathogen bacteria. We also proved the importance of the glycosylation of the S-layer protein from Lb. kefir in the binding to the receptor and the thus far inhibition of infection.This novel characteristic of the S-layers, a GRAS status protein, from microorganism widely use in food industry may  contribute to the pathogen exclusion already reported for these probiotic strains and might be applied as a novel enzymatic antimicrobial agents as well as an inhibitor of bacterial infection and entry to the cells.","palabras_clave":"s-layer|dc-sign"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6664,"idioma_id":5,"titulo":"El papel del Estado en torno a las pymes industriales: un análisis desde la economía regional pos crisis 2001","resumen":"Luego de un proceso recesivo y de crisis económica y social, el proceso de reactivación económica a partir de los años 2003\/4 propició un escenario favorable para la actividad de las pymes industriales de la economía regional. Nos preguntamos, en ese marco, cuál fue el papel del Estado-gobierno, en qué aspectos aparece acompañando dicha dinámica.","palabras_clave":"PYMES|INDUSTRIA|PAPEL DEL ESTADO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6665,"idioma_id":4,"titulo":"Selective Oestrogen receptor agonists rescued hippocampus parameters in male spontaneously hypertensive rats","resumen":"Spontaneously hypertensive rats (SHR) show pronounced hippocampusalterations, including low brain derived neurotropic factor expression, reducedneurogenesis, astrogliosis and increased aromatase expression. These changesare reverted by treatment with 17b-oestradiol. Toelucidate which oestradiol receptor (ER) type is involved in these neuroprotectiveeffects, we used agonists of the ERa (propylpyrazoletriol ,PPT) and the ERb (diarylpropionitrite, DPN) givenduring 2 weeks to 4 month old male SHR. Wistar Kyoto (WKY) normotensive ratsserved as controls. Using immunocytochemistry, we determined glial fibrillaryprotein (GFAP)+ astrocytes in the CA1, CA3 and hilus of the dentate gyrus ofhippocampus, aromatase immunostaining in the hilus and doublecortin (DCX) +neuronal progenitors in the inner granular zone of the dentate gyrus. BDNF mRNAwas also measured in hippocampus by qPCR. The results showed that PPT had noeffect on blood pressure of SHR, decreased astrogliosis, and increasedaromatase staining, slightly increased BDNF mRNA but had no effect on thenumber of DCX+ progenitors. Treatment with DPN decreased blood pressure of SHR,decreased astrogliosis, increased BDNF mRNA and DCX+ progenitors but did notmodify aromatase staining. We hypothesize that while both receptor types mayparticipate in the previously reported beneficial effects of 17b-oestradiol in SHR, receptor activation with DPN may preferentiallyfacilitate BDNF mRNA expression and neurogenesis. This study may help to designER-based neuroprotection for the encephalopathy of hypertension.","palabras_clave":"oestrogen receptors|hippocampus|hypertension|neurogenesis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6666,"idioma_id":4,"titulo":"Carbonic Anhydrase Inhibitors Reduce Cardiac Dysfunction after Sustained Coronary Artery Ligation in Rats","resumen":"Background- Two potent carbonic anhydrase (CA) inhibitors withwidely differing membrane permeability, poorly diffusible benzolamide(BZ) and highly diffusible ethoxzolamide (ETZ) were assessed to determinewhether they can reduce cardiac dysfunction in rats subjected to coronaryartery ligation (CAL)-induced myocardial infarction.Methods and results- Rats with evidence of heart failure (HF) at 32 weeksfollowing a permanent left anterior coronary artery occlusion weretreated with placebo, BZ or ETZ (4 mg.kg.day-1) for 4-weeks at which timeleft ventricular function and structure were evaluated. Lung weight\/bodyweight (LW\/BW) ratio increased in CAL rats by 17±1% vs. control,suggesting pulmonary edema. There was a trend for BZ and ETZ toameliorate the increase in LW\/BW by almost 50% (9±5% and 9±8%,respectively, versus CAL) (P=0.16, NS). Echocardiographic assessmentshowed decreased LV midwall shortening in HF rats, 21±1% vs. control32±1%, which was improved by BZ to 29±1% and ETZ to 27±1%, and reducedendocardial shortening in HF rats 38±3% vs. control 62±1%, partiallyrestored by BZ and ETZ to ~50%. Expression of the hypoxia-induciblemembrane-associated CAIX isoform increased by ~60% in HF rat hearts, andthis effect was blocked by ETZ.Conclusions- We conclude that CAL-induced myocardial interstitialfibrosis and associated decline in left ventricular function werediminished with BZ or ETZ treatment. The reductions in cardiacremodelling in HF with both ETZ and BZ CA inhibitors suggest thatinhibition of a membrane-bound CA appears to be the critical site forthis protection.","palabras_clave":"carbonic anhydrase|myocardial infarction"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6667,"idioma_id":4,"titulo":"Pollen development and anther morphology in 14 species of Rhamnaceae","resumen":"Microsporogenesis, microgametogenesis, and anther structure of 14 species belonging to Rhamneae (Rhamnoids clade), Paliureae, Pomaderreae, Colletieae and Gouanieae (Ziziphoids clade) of the Rhamnaceae family were analyzed using bright-field, scanning, and transmission electron microscopy. The aim is to look for morphological characters that have systematic value on a suprageneric level within the family.  The character states of the number of nuclei in tapetal cells, the endothecial thickening pattern, and the number of endothecial layers, are consistent on tribal level. Tribes were previously delineated based on molecular phylogenetic evidence.  On the other hand, pollen morphology does not seem to have systematic value at tribal level in Rhamnaceae. The presence or absence of orbicules and their morphology vary within tribes. It seems that for Rhamnaceae, the anatomy of the reproductive sporophytic structures could have more systematic value than the gametophytic structures. However, more studies are needed to confirm this.","palabras_clave":"MICROSPORANGIUM|POLLEN| RHAMNACEAE|ORBICULES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6668,"idioma_id":4,"titulo":"New insights about flocculation process in sodium caseinate-stabilized emulsions","resumen":"Flocculation process was studied in emulsions formulated with 10 wt.% sunflower oil, 2, 5 or 7.5 wt.% NaCas, and with or without addition of sucrose (0, 5, 10, 15, 20 or 30 wt.%). Two different processing conditions were used to prepare emulsions: ultraturrax homogenization or further homogenization by ultrasound. Emulsions with droplets with diameters above (coarse) or below (fine) 1 &#956;m were obtained. Emulsions were analyzed for droplet size distribution by static light scattering (SLS), stability by Turbiscan, and structure by confocal laser scanning microscopy (CLSM) and small angle X-ray scattering (SAXS). SAXS data were fitted by a theoretical model that considered a system composed of poly dispersed spheres with repulsive interaction and presence of aggregates. Flocculation behavior was caused by the self-assembly properties of NaCas, but the process was more closely related to interfacial protein content than micelles concentration in the aqueous phase. The results indicated that casein aggregation was strongly affected by disaccharide addition, hydrophobic interaction of the emulsion droplets, and interactions among interfacial protein molecules. The structural changes detected in the protein micelles in different environments allowed understanding the macroscopic physical behavior observed in concentrated NaCas emulsions.","palabras_clave":"NaCas|Flocculation|SAXS|Emulsion"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6669,"idioma_id":4,"titulo":"Cnbp ameliorates Treacher Collins Syndrome craniofacial anomalies through a pathway that involves redox-responsive genes","resumen":"Treacher-CollinsSyndrome (TCS) is a rare congenital disease (1:50000 live births) characterizedby craniofacial defects, including hypoplasia of facial bones, cleft palate andpalpebral fissures. Over 90% of the cases are due to mutations in the TCOF1 gene, which codifies the nucleolarprotein Treacle. Here we report a novel TCS-like zebrafish model displaying featuresthat fully recapitulate the spectrum of craniofacial abnormalities observed in patients.As it was reported for a Tcof1+\/&#8722; mouse model, Treacle depletion inzebrafish caused reduced rRNA transcription, stabilization of Tp53 and increasedcell death in the cephalic region. An increase of ROS along with theoverexpression of redox-responsive genes was detected; furthermore, treatmentwith antioxidants ameliorated the phenotypic defects of craniofacial anomaliesin TCS-like larvae. On the other hand, Treacle depletion led to a lowering inthe abundance of Cnbp, a protein required for proper craniofacial development. Tcof1-knockdown in transgenic zebrafishover-expressing cnbp resulted in barelyaffected craniofacial cartilage development, reinforcing the notion that Cnbpplays a role in the pathogenesis of TCS. The cnbp overexpression rescued the TCS phenotype in a dose-dependentmanner by a ROS-cytoprotective action that prevented the redox-responsive genesup-regulation but did not normalize the synthesis of rRNAs. Finally, a positivecorrelation between the expression of CNBPand TCOF1 in mesenchymal cells from bothcontrol and TCS subjects was found. Based on this, we suggest CNBP as an additionaltarget for new alternative therapeutic treatments to reduce craniofacialdefects not only in TCS but also in other neurocristopathies.","palabras_clave":"ZEBRAFISH|TREACHER-COLLINS SYNDROME|CNBP|ROS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6671,"idioma_id":6,"titulo":"Universal Pinning Energy Barrier for Driven Domain Walls in Thin Ferromagnetic Films","resumen":"We report a comparative study of magnetic field driven domain wall motion in thin films made of different magnetic materials for a wide range of field and temperature. The full thermally activated creep motion, observed below the depinning threshold, is shown to be described by a unique universal energy barrier function. Our findings should be relevant for other systems whose dynamics can be modeled by elastic interfaces moving on disordered energy landscapes.","palabras_clave":"domain walls|creep dynamics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6672,"idioma_id":4,"titulo":"Correlation between Structural, Chemical, and Electrochemical Properties of  La0.6Sr0.4CoO3&#8722;d Nanopowders for Application in Intermediate Temperature Solid Oxide Fuel Cells","resumen":"Lanthanum&#8722;strontium cobaltites are excellent mixed ionic andelectronic conductors and exhibit good activity for the oxygen reductionreaction (ORR) at temperatures higher than 500 °C. This led to these materials beingproposed as cathodes for solid-oxide fuel cell devices, either for the standardhigh temperature (SOFC, 900&#8722;1000 °C) or theintermediate temperature (IT-SOFC, 500&#8722;700 °C) range. Although it was determined that crystalstructure and oxygen vacancies play a central role in these properties, littleis known about cobalt speciation at SOFC or IT-SOFC working temperatures. Inthis study the cobalt speciation and local order of La0.6Sr0.4CoO3&#8722;d (LSC) is analyzed in detailfor the first time in nano- and microstructured powders by means of in situexperiments using X-ray absorption spectroscopy (XAS) in the temperature rangeof 20&#8722;700 °C. The electrochemical performance of LSC-based cathodes in symmetricalcells under the same conditions is also studied using electrochemical impedancespectroscopy (EIS). Cobalt atoms can be found in any of the three possibleoxidation states. Their relative amount depends on temperature and crystallitesize. In crystallites of a few tens of nanometers, the ease with which Co atomschange their oxidation state is enhanced. This effect probably facilitates oxide transport in LSC-based cathodes.","palabras_clave":"LSC|CATHODE|IT-SOFC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6673,"idioma_id":4,"titulo":"Anxiety response and restraint-induced stress differentially affect ethanol intake in female adolescent rats","resumen":"Anxiety disorders are more likely to occur in women than in men, usually emerge during adolescence and exhibit high comorbidity with alcohol use disorders (AUD). Adolescents with high levels of anxiety or heightened reactivity to stress may be at-risk for developing AUD. An approach to analyze if high levels of inborn anxiety predict greater ethanol drinking is to assess the latter variable in subjects classified as high- or low-anxiety responders. The present study assessed ethanol drinking in adolescent, female Wistar, rats classified as high-, low- or average-anxiety responders and exposed or not to restraint stress (RS, Exp. 1). Classification was made through a multivariate index derived from testing anxiety responses in an elevated plus maze and a light-dark box tests. RS was applied after animals had been initiated to ethanol drinking. Intake of sweetened ethanol was unaffected by level of anxiety response. Adolescents with high levels of inborn anxiety exhibited significantly higher intake of unsweetened ethanol than counterparts with standard levels of anxiety, yet this effect was inhibited by RS exposure. Experiment 2 assessed FOS immunoreactivity after RS. Stress induced a significant increase in FOS immunoreactivity at the paraventricular nucleus, yet this effect was unaffected by level of anxiety response. Female adolescents with high levels of basal anxiety may be at-risk for exhibiting increased predisposition for ethanol intake and preference. The study also indicates that stress may exert differential effects on adolescent ethanol intake as a function of the level of anxiety response.","palabras_clave":"ETHANOL INTAKE|ADOLESCENCE|STRESS|ANXIETY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6674,"idioma_id":5,"titulo":"La intolerancia","resumen":"A partir de experiencias concretas de conflictos sociales el texto se propone un análisis de los mismos en función de algunos ejes teóricos propuestos por la teoría psicoanalítica: la función del Ideal del Yo en el sujeto y en la masa, y los efectos de identificación y segregación.","palabras_clave":"Identificación|Ideal del Yo|Psicología de las Masas"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6675,"idioma_id":4,"titulo":"Shape memory epoxy vitrimers based on DGEBA crosslinked with dicarboxylic acids and their blends with citric acid","resumen":"Thermosetting polymers were synthesized from a commercial epoxy resin (diglycidyl ether of bisphenol A, DGEBA) and a tricarboxylic (citric, CA) and different dicarboxylic (sebacic, SA and glutaric, GA) acids. Crosslinking of DGEBA-SA and DGEBA-GA systems was achieved by using an epoxy excess that was homopolymerized after consumming all the acid groups. It was found that the material properties depend on the diacid length and on the epoxy excess and that, with the proper formulation, vitrimers with Tg values ranging from 51ºC to 62ºC, and a high rate of stress relaxation (less than 1h at 160ºC to achieve 63% of relaxation) could be obtained. Notably, using a mixture of tri-functional CA with SA allowed to reduce the epoxy excess while keeping a high Tg value and speeding up the stress relaxation. Three of the formulations were selected and their shape memory performance was studied. Good shape fixity and shape recovery ratios (&gt;99%) were obtained, indicating a good shape memory performance overall. These properties can be used to create different permanent and temporary shapes on a thermosetting polymer obtained from widely available and affordable raw materials.","palabras_clave":"Vitrimer|Epoxy|Shape memory"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6676,"idioma_id":4,"titulo":"Magnetic   nickel   ferrite   nanoparticles   for   removal   of   dipyrone   from aqueous   solutions","resumen":"Magneticnickel ferrite nanoparticles with a size of 20±3 nm have been synthesized by aco-precipitation method at room temperature. Crystalline structured particleswith ferromagnetic properties were obtained by calcination at 600 C. These nanoparticleswere characterized in order to determine the particle size distribution,morphology, surface charge and adsorption properties. Nickel ferrite nanoparticleswere tested as potential adsorbent for removal of dipyrone from aqueoussolution by evaluating adsorption kinetics and isotherms. The maximum adsorptioncapacities at 20 C were 30.4 mg g-1 (pH 6) and 25.0 mg g-1(pH 4). Additionally, analytical solution of the Langmuir adsorption kineticequation was successfully applied. Desorption experiments were conducted at labscale by using a flowthrough system aiming to study the release of dipyrone from the proposedmaterial at different experimental conditions. A 55% of desorption forpre-sorbed DIP is achieved by performing several extraction cycles with anaqueous solution (pH 2.5) at 50 C. Also, 43% desorption was obtained by adding 0.2M phosphate, and 48% by adding 0.2 M sulfate. With the advantages of low costand rapid processing, these magnetic nanoparticles could gain a promisingapplication in the removal of pharmaceutical compound residues during treatmentof water samples on a large scale.","palabras_clave":"ADSORPTION|EMERGING POLLUTANTS|PHARMACEUTICALS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6677,"idioma_id":4,"titulo":"Insulin effects on honeybee appetitive behavior","resumen":"Worker honeybees (Apis mellifera L.) perform different tasks throughout their adult lifespan. It has been proposed that the insulin\/insulin-like signalling pathway contributes to age related changes in task performance of eusocial insects. Insulin levels increase as the honeybee worker ages from nurse to food processor to forager. As transition between tasks implies a differential use of sensory modalities, we wished to evaluate the effect of insulin signalling on olfactory and gustatory responsiveness as well as on appetitive olfactory learning in preforaging honeybee workers. Adults of known age reared either in the laboratory or in the hive were abdominally injected with 1 µl of insulin or Hepes buffer (control). Immediately after, and focussing on the proboscis extension response, spontaneous response toward odours, sucrose responsiveness and olfactory learning were tested. Insulin injected bees have higher spontaneous odour responses, independently of the environment they were reared in or their age. Sucrose responsiveness and odour discrimination during PER conditioning are affected by insulin differently according to age; whereas insulin increases gustatory responsiveness and diminishes learning abilities of younger workers, it has the opposite effect on older bees. As a summary, insulin can improve chemosensory responsiveness in young workers, but also worsens their learning abilities to discriminate odours. Thus, the insulin signalling pathway would be readily mature in young workers, although they are not yet initiating outdoor activities. These results show a strong effect of insulin signalling on appetitive behaviour, which reinforces the variable behavioural repertoire found in the honeybee worker caste throughout adulthood.","palabras_clave":"honeybee|appetitive behavior|insulin|cognitive abilities"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6678,"idioma_id":4,"titulo":"An appetitive experience after fear memory destabilization attenuates fear retention: involvement GluN2B-NMDA receptors in the Basolateral Amygdala Complex.","resumen":"It is known that a consolidated memory can return to a labile state and become transiently malleable following reactivation.This instability is followed by a restabilization phase termed reconsolidation. In this work, we explored whether an unrelatedappetitive experience (voluntary consumption of diluted sucrose) can affect a contextual fear memory in rats during thereactivation-induced destabilization phase. Our findings show that exposure to an appetitive experience following reactivationcan diminish fear retention. This effect persisted after 1 wk. Importantly, it was achieved only under conditions thatinduced fear memory destabilization. This result could not be explained as a potentiated extinction, because sucrose wasunable to promote extinction. Since GluN2B-containing NMDA receptors in the basolateral amygdala complex (BLA)have been implicated in triggering fear memory destabilization, we decided to block pharmacologically these receptorsto explore the neurobiological bases of the observed effect. Intra-BLA infusion with ifenprodil, a GluN2B-NMDA antagonist,prevented the fear reduction caused by the appetitive experience. In sum, these results suggest that the expression of afear memory can be dampened by an unrelated appetitive experience, as long as memory destabilization is achieved duringreactivation. Possible mechanisms behind this effect and its clinical implications are discussed.","palabras_clave":"AMYGDALA|FEAR-MEMORY|APPETITIVE-EXPERIENCE|GLUN2B-NMDA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6679,"idioma_id":4,"titulo":"Influence of maturity condition and habitat type on food resources utilization by Octopus tehuelchus in Atlantic Patagonian coastal ecosystems","resumen":"This study evaluates differences in Octopustehuelchus diet in relation to sex and maturity condition(juvenile, mature, post-spawning) in two contrasting habitattypes (sandy vs. rocky). Muscle of O. tehuelchus andof its associated food sources were analyzed for &#948;13C and&#948;15N. There was no correlation between &#948;13C or &#948;15N andoctopus size. &#948;13C and &#948;15N values were similar betweensexes indicating similar diets. The Bayesian mixing model(MixSIAR) showed that the proportional contribution ofthe sources varied with the maturity condition of octopuses,and between habitat types. Our results show differences inthe proportions of alternative prey in the diet of O. tehuelchusrelated to their physiological condition and the habitatcharacteristics. The differential food source utilizationmay be related to differential prey energy contribution andcould explain the differences in the growth pattern and lifespan, as well as in the reproductive traits and recruitmentsuccess of O. tehuelchus in sandy and rocky habitats. Ourresults highlight the importance of considering such differencesin octopus diet, since they may have consequences","palabras_clave":"food resources|maturirty|Octopus|Patagonia"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6680,"idioma_id":4,"titulo":"TMV induces RNA decay pathways to modulate gene silencing and disease symptoms","resumen":"RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in geneexpression, development and defense responses in eukaryotes. These mechanisms are known as the RNAQuality Control or RQC pathways. In plants, another important RNA degradation mechanism is the post-transcriptional gene silencing (PTGS) mediated by small RNAs (siRNAs). Notably, the RQC pathway antago-nizes PTGS by preventing the entry of dysfunctional mRNAs into the silencing pathway to avoid globaldegradation of mRNA by siRNAs. Viral transcripts must evade RNA degrading mechanisms, thus virusesencode PTGS suppressor proteins to counteract viral RNA silencing. Here, we demonstrate that tobaccoplants infected with TMV and transgenic lines expressing TMV MP and CP (coat protein) proteins (whichare not linked to the suppression of silencing) display increased transcriptional levels of RNA decay genes.These plants also showed accumulation of cytoplasmic RNA granules with altered structure, increased ratesof RNA decay for transgenes and defective transgene PTGS ampli&#64257;cation. Furthermore, knockdown ofRRP41 or RRP43 RNA exosome components led to lower levels of TMV accumulation with milder symptomsafter infection, several developmental defects and miRNA deregulation. Thus, we propose that TMV pro-teins induce RNA decay pathways (in particular exosome components) to impair antiviral PTGS and thisdefensive mechanism would constitute an additional counter-defense strategy that lead to diseasesymptoms","palabras_clave":"RNA decay|exosome|TMV|defense|symptoms|PTGS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6681,"idioma_id":4,"titulo":"Isotopic trends in the diets of hunter-gatherers of the lower Paraná wetland, South America","resumen":"The results of the isotopic analysis of 23 individuals recovered in the lower Paraná wetland, South America, are presented. The samples were recovered from complex hunter-gatherer sites ranging from the years 1800 to 600 BP. Two isotopic trends were identified. The first one is based on depleted proteins with high trophic levels, related to freshwater fish consumption. The second one is similar, but includes a moderate intake of carbohydrates. The regional tendency was focused on fishing resources over time, and not on the alleged importance of cultigens. The data obtained from infants and children suggest an early period of weaning and age-based food allocation for infants based on depleted carbs. The data obtained are compared to other results published for humans recovered from sites of other archaeological units distributed throughout the same region.","palabras_clave":"ISOTOPES|COMPLEX HUNTER-GATHERERS|SOUTH AMERICA|FISHING|WEANING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6683,"idioma_id":4,"titulo":"COMBINED EFFECTS OF VERTICAL AND LATERAL CONFINEMENT ON THE MAGNETIC PROPERTIES OF MNAS MICRO AND NANO-RIBBONS","resumen":"X-ray photoemission electron microscopy (XPEEM) has been used for imaging magnetic domains in lithographically fabricated MnAs ribbons, addressing the dependence of the domain configuration on film thickness and ribbon width. Our experiments show a transition from head-on to regular stripe domains below a critical width\/thickness ratio wc&#8776;6. Micromagnetic simulations suggest that this transition is correlated to the magnetic structure of the surface plane. Depending on the ribbon width and thickness, the magnetic configuration is shown to evolve from flux-closure domain structure to a state of almost homogeneous magnetization, observed for narrower ribbons. The evolution of the domain structure, magnetic fraction and magnetization with temperature has been studied across the ferromagnetic\/paramagnetic transition. Our experiments show that the magnetic configuration in ribbons exhibits higher stability to temperature variations than in as-cast films.","palabras_clave":"HIBRID METAL SEMICONDUCTOR STRUCTURES|MAGNETIC DOMAINS|XPEEM|MIGROMAGNETIC SIMULATIONS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6684,"idioma_id":4,"titulo":"Fusion of foreign T-cell epitopes and addition of TLR agonists enhance immunity against Neospora caninum profilin in cattle","resumen":"We demonstrated recently that immunization with recombinant Neospora caninum profilin (rNcPRO) induces limited protection and a regulatory T-cell response in mice. The aim of this study was to evaluate the immune response elicited by rNcPRO in cattle and assess a strategy to enhance its immunogenicity, combining the addition of T-cell epitopes and immune modulators. We developed a chimeric recombinant profilin fused to functional T-cell epitopes present in the N-terminal sequence of vesicular stomatitis virus (VSV) glycoprotein G (rNcPRO\/G). Groups of three cattle were immunized with two doses (2 weeks apart) of rNcPRO or rNcPRO\/G formulated with alum hydroxide or a nanoparticulated soya-based adjuvant enriched with Toll-like receptor (TLR) 2 and TLR9 agonists, aimed to tackle the MyD88 pathway (AVECplus). rNcPRO induced only a primary immune response (IgM mediated), while antibodies in rNcPRO\/G-vaccinated animals switched to IgG1 after the booster. The vaccine formulated with rNcPRO\/G and AVECplus improved the production of systemic IFN-&#947; and induced long-term recall B-cell responses. Overall, our study provides data supporting the use of T-cell epitopes from VSV glycoprotein G and TLR agonists to enhance and modulate immunity to peptide antigens in bovines, particularly when using small proteins from parasites for which immune responses are usually feeble.","palabras_clave":"NEOSPORA CANINUM|PROFILIN|RECOMBINANT VACCINES|CATTLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6685,"idioma_id":4,"titulo":"Demographic variation in timing and intensity of feather molt in migratory Fork-tailed Flycatchers (Tyrannus s. savana)","resumen":"Understanding the annual cycle of migratory birds is imperative for evaluating the evolution of life-history strategies and developing effective conservation strategies. Yet, we still know little about the annual cycle of migratory birds that breed at south-temperate latitudes of South America. We aged, sexed, and determined the progression and intensity of body, remige, and rectrix molt of migratory Fork-tailed Flycatchers (Tyrannus s. savana) at breeding sites in southern South America and at wintering sites in northern South America. Molt of both body and flight feathers occurred primarily during the winter. In early winter, a similar proportion of young and adult flycatchers molted remiges and rectrices, but remige molt intensity (number of remiges molting) was greater and primary molt progression (mean primary feather molting) more advanced in adults. In late winter, remige molt intensity and primary molt progression did not differ between age groups. We found no difference between males and females either in the proportion of individuals molting in winter or in the intensity or progress of remige molt. Our results suggest that the nominate subspecies of Fork-tailed Flycatcher undergoes one complete, annual molt on the wintering grounds, and represents the first comprehensive evaluation of molt timing of a migratory New World flycatcher that overwinters in the tropics. Given that breeding, molt, and migration represent three key events in the annual cycle of migratory birds, knowledge of the timing of these events is the first step toward understanding the possible tradeoffs migratory birds face throughout the year.","palabras_clave":"Argentina|Colombia|Plumas|Rectrices|Muda"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6686,"idioma_id":5,"titulo":"RELACIÓN ENTRE MINERALIZACIÓN DE Cu Y BITUMEN EN EL PROSPECTO LA CUPROSA, DORSAL DE HUINCUL, NEUQUÉN","resumen":"La Cuprosa is a Revett or sandstone sub-type sediment-hosted stratiform Cu deposit of the Portezuelo Formation (Neuquén Group), located over the Aguada Baguales oil field, Huincul Ridge. Its diagenetic story can be subdivided in: diagenesis, mineralization and telogenesis. During early diagenesis hematite and kaolinite precipitated from meteoric waters. Early oxidation of hydrocarbon in depth, rising CO2 and mixing with meteoric waters produced calcite I precipitation. The incoming of liquid hydrocarbons and redox reactions with the rock generated: clasts and previous cements dissolution, porosity increase, smectite ± chlorite and sintaxial micro-quartz cements, reduction of Fe3+ to Fe2+ to form pyrite (± chlorite), and later analcime and baroque calcite II precipitation. Circulation of slightly oxidizing, neutral, Cu-rich basinal brines could extract Cu from subjacent red beds and igneous rocks, transport it in solution, and precipitate Cu-Fe sulfides in contact with hydrocarbons. These sulfides replaced pyrite and generated partial iron remobilization to the periphery of the mineralization zone. Andean tectonic during the Miocene produced the telogenetic processes: calcite III formation, and Cu sulfides replacement by malachite> chrysocolla> azurite> brochantite? and pyrite by hematite> goethite. The bleaching distribution mapped by satellite imagery analysis and the zonation of the alteration can be utilized for hydrocarbon migration and similar stratiform Cu deposits studies.","palabras_clave":"SEDIMENT HOSTED CU DEPOSIT|NEUQUEN GROUP|HYDROCARBONS|BLEACHING"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6687,"idioma_id":4,"titulo":"Hexachlorobenzene modulates the crosstalk between the Aryl Hydrocarbon Receptor and Transforming Growth Factor &#946;1 signaling, enhancing human breast cancer cell migration and invasion","resumen":"Given the number of women affected by breast cancer, considerable interest has been raised in understanding the relationships between environmental chemicals and disease onset. Hexachlorobenzene (HCB) is a dioxin-like compound that is widely distributed in the environment and is a weak ligand of the aryl hydrocarbon receptor (AhR). We previously demonstrated that HCB acts as an endocrine disruptor capable of stimulating cell proliferation, migration, invasion, and metastasis in different breast cancer models. In addition, increasing evidence indicates that transforming growth factor-b1 (TGF-b1)can contribute to tumor maintenance and progression. In this context, this work investigated the effect of HCB (0.005, 0.05, 0.5, and 5 mM) on TGF-b1 signaling and AhR\/TGF-b1 crosstalk in the human breast cancer cell line MDA-MB-231 and analyzed whether TGF-b1 pathways are involved in HCB-induced cellmigration and invasion. RT-qPCR results indicated that HCB reduces AhR mRNA expression through TGFb1 signaling but enhances TGF-b1 mRNA levels involving AhR signaling. Western blot analysis demonstrated that HCB could increase TGF-b1 protein levels and activation, as well as Smad3, JNK, and p38 phosphorylation. In addition, low and high doses of HCB were determined to exert differential effects on AhR protein levels, localization, and activation, with a high dose (5 mM) inducing AhR nuclear translocation and AhR-dependent CYP1A1 expression. These findings also revealed that c-Src and AhR are involved in HCB-mediated activation of Smad3. HCB enhances cell migration (scratch motility assay) andinvasion (Transwell assay) through the Smad, JNK, and p38 pathways, while ERK1\/2 is only involved in HCB-induced cell migration. These results demonstrate that HCB modulates the crosstalk between AhR and TGF-b1 and consequently exacerbates a pro-migratory phenotype in MDA-MB-231 cells, which contributes to a high degree of malignancy. Taken together, our findings help to characterize themolecular mechanism underlying the effects of HCB on breast cancer progression.","palabras_clave":"HEXACHLOROBENZENE|BREAST CANCER|ARYL HYDROCARBON RECEPTOR|TRANSFORMING GROWTH FACTOR RECEPTOR-beta 1|MDA-MB-231 BREAST CANCER CELL LINE|INVASION|MIGRATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6688,"idioma_id":4,"titulo":"Stellar populations in the Carina region. The Galactic plane at l = 291","resumen":"Context. Previous studies of the Carina region have revealed itscomplexity and richness as well as a significant number of early-typestars. However, in many cases, these studies only concentrated on thecentral region (Trumpler 14\/16) or were not homogeneous. This latteraspect, in particular, is crucial because very different ages anddistances for key clusters have been claimed in recent years. Aims: The aim of this work is to study in detail an area of the Galacticplane in Carina, eastward &#951; Carina. We analyze the properties ofdifferent stellar populations and focus on a sample of open clusters andtheir population of young stellar objects and highly reddened earlystars. We also studied the stellar mass distribution in these clustersand the possible scenario of their formation. Finally, we outline theGalactic spiral structure in this direction.  Methods: We obtaineddeep and homogeneous photometric data (UBVIKC) for six youngopen clusters: NGC 3752, Trumpler 18, NGC 3590, Hogg 10, 11, and 12,located in Carina at l ~ 291°, and their adjacent stellar fields,which we complemented with spectroscopic observations of a few selectedtargets. We also culled additional information from the literature,which includes stellar spectral classifications and near-infraredphotometry from 2MASS. We finally developed a numerical code thatallowed us to perform a homogeneous and systematic analysis of the data.Our results provide more reliable estimates of distances, colorexcesses, masses, and ages of the stellar populations in this direction. Results: We estimate the basic parameters of the studied clustersand find that they identify two overdensities of young stellarpopulations located at about 1.8 kpc and 2.8 kpc, with EB - V~ 0.1 - 0.6. We find evidence of pre-main-sequence populations insidethem, with an apparent coeval stellar formation in the most conspicuousclusters. We also discuss apparent age and distance gradients in thedirection NW-SE. We study the mass distributions of the covered clustersand several others in the region (which we took form the literature).They consistently show a canonical IMF slope (the Salpeter one). Wediscover and characterise an abnormally reddened massive stellarpopulation, scattered between 6.6 and 11 kpc. Spectroscopic observationsof ten stars of this latter population show that all selected targetswere massive OB stars. Their location is consistent with the position ofthe Carina-Sagittarius spiral arm.","palabras_clave":"open clusters and associations: general|Galaxy: structure|stars: early-type|stars: pre-main sequence|stars: formation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6689,"idioma_id":4,"titulo":"Towards sustainable biofuel production: Design of a new biocatalyst to biodiesel synthesis from waste oil and commercial ethanol","resumen":"The sustainable technologies to produce alternative energies as biofuel with focus on the harnessing ofrenewable sources and waste biomass is gradually gaining ground. Although the biodiesel is a veryattractive biofuel its production from vegetable oils competes with the feed generation with theconsequent socio-economic costs involved. Therefore, the reuse of waste oils appears as an alternativehighly promising. However the high content of free fatty acid and water in this raw material difficult itsuse in the conventional processes employed in the actuality. In this work, we successfully develop hybridcatalysts based in Pseudomonas fluorescens lipase immobilized over Ca and Na modified mesoporousSBA-15 supports. The physic-chemical properties of the supports were determined by Small-angle X-rayScattering (SAXS), Transmission Electron Microscopy (TEM), Scanning Electron Microscope (SEM),Infrared Spectroscopy (IR), Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP) and N2adsorption measurements. These hybrid catalysts were capable to process waste oils, and even, commercialethanol (96%) obtained from a fermentative process An optimum activity, with around 90% ofFAEE yield, was achieved with lipase immobilized on Ca modified SBA-15 using 4% of water respect to oil,1:4 oil\/ethanol ratio, 400 mg\/g of enzyme immobilized, at 37 C and 180 oscillations\/min. This catalystcould contribute to development of a more environmentally and economically viable process to biodieselproduction.","palabras_clave":"Biodiesel | Enzymatic immobilization SBA-15 |Biocatalysis|Alternative energies"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6690,"idioma_id":5,"titulo":"Calidad de la materia orgánica y disponibilidad de macro y  micronutrientes por la inclusion de trigo como cultivo de cobertura","resumen":"Los cultivos de cobertura aparecen como una opción que permite mejorar el balance de nutrientes y carbono en el suelo (C).El objetivo del presente trabajo fue determinar el efecto de la incorporación del trigo como cultivo de cobertura (CC) sobre ladinámica de la materia orgánica en el suelo y la disponibilidad de los principales macro y micronutrientes bajo diferentes rotacionesde cultivos. Este trabajo se realizó en un ensayo de larga duración bajo siembra directa. Los tratamientos fueron: sojasoja(S-S), soja-cultivo de cobertura-soja (S-CC-S), maíz-soja-trigo\/soja (M-S-T\/S) y maíz-cultivo de cobertura-soja-trigo\/soja(M-CC-S-T\/S). Como principales resultados, se pudo observar que la fracción de carbono orgánico comprendida entre los 53y 105 mm (COPf), se incrementó significativamente cuando se incorporaron los CC al monocultivo de soja. El C menor a 53mm (COa), fue significativamente mayor cuando se incorporó el CC a la rotación con predominio de gramíneas y no así enel caso del monocultivo de soja. El monocultivo de soja presentó un promedio de 11,1 g kg-1 de carbono orgánico particuladototal (COPg + COPf), siendo el valor más bajo y significativamente menor que para S-CC-S (17 g kg-1). No se observaron diferenciassignificativas en la concentración de macronutrientes. En el caso de los micronutrientes, se pudo observar que las rotacionescon predominio de gramíneas sin CC tienen una mayor concentración de cinc (Zn) y manganeso (Mn) (2,2 y 63 mg kg-1) queel monocultivo de soja en los primeros 5 cm de suelo (1,4 y 50 mg.kg-1 respectivamente). La concentración de estos micronutrientesademás estuvo positivamente correlacionada con mayores contenidos de materia orgánica. El CC no pareció haberafectado la concentración de micronutrientes para este año de muestreo por lo que sucesivos análisis deberán realizarse paraconocer su dinámica en el tiempo.","palabras_clave":"materia organica|cinc|rotaciones|cultivo cobertura"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6691,"idioma_id":4,"titulo":"Neural regeneration dynamics of Xenopus laevis olfactory epithelium after zinc sulfate-induced damage","resumen":"Neural stem cells (NSCs) of the olfactory epithelium (OE) are responsible for tissue maintenance and the neural regeneration after severe damage of the tissue. In the normal OE, NSCs are located in the basal layer, olfactory receptor neurons (ORNs) mainly in the middle layer, and sustentacular (SUS) cells in the most apical olfactory layer. In this work, we induced severe damage of the OE through treatment with a zinc sulfate (ZnSO4) solution directly in the medium, which resulted in the loss of ORNs and SUS cells, but retention of the basal layer. During recovery following injury, the OE exhibited increased proliferation of NSCs and rapid neural regeneration. After 24 h of recovery, new ORNs and SUS cells were observed. Normal morphology and olfactory function were reached after 168 h (7 days) of recovery after ZnSO4 treatment. Taken together, these data support the hypothesis that NSCs in the basal layer activate after OE injury and that these are sufficient for complete neural regeneration and olfactory function restoration. Our analysis provides histological and functional insights into the dynamics between olfactory neurogenesis and the neuronal integration into the neuronal circuitry of the olfactory bulb that restores the function of the olfactory system.","palabras_clave":"olfactory epithelium|chemical damage|neuronal regeneration|xenopus laevis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6692,"idioma_id":5,"titulo":"La Argentina y la Alianza del Pacífico. Riesgos y Oportunidades de una nueva geopolítica","resumen":"En los últimos años, a partir del surgimiento de la Alianza del Pacífico y la división de la cartografía del regionalismo como una disputa entre un eje Atlántico y otro Pacífico, se ha puesto en discusión un viejo dilema de la inserción internacional de los países: acuerdos de libre comercio vis-a-vis el fortalecimiento del mercado interno con proyección regional. Este es el caso de Argentina a partir del gobierno de Mauricio Macri, que plantea el ingreso del país a la Alianza del Pacífico, impulsando en principio al MERCOSUR en esa dirección. Por lo tanto, este trabajo analiza las posibilidades formales-legales y económicas de un posible ingreso de la Argentina a la Alianza del Pacífico. Primero: ¿sería posible la pertenencia a estos dos esquemas de integración? Segundo: ¿en que se beneficiaría concretamente la Argentina con su incorporación a dicha Alianza? Para ello se realiza un pequeño ejercicio comparativo para observar en qué medida y a qué sectores de los países miembros de la Alianza del Pacífico benefició dicho acuerdo, planteando algunas líneas de lo que podría pasar en virtud de lo analizado en la Argentina.","palabras_clave":"Alianza del Pacifico|MERCOSUR|INTEGRACION|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6693,"idioma_id":4,"titulo":"Mitochondrial nitric oxide production supported by reverse electron transfer","resumen":"Heart phosphorylating electron transfer particles (ETPH) produced NO at 1.2 ± 0.1 nmol NO. min-1. mg protein-1 by the mtNOS catalyzed reaction. These particles showed a succinate-NAD+ reductase activity of 64 ± 3 nmol. min-1. mg protein-1 sustained by reverse electron transfer (RET) at expenses of ATP and succinate. The same particles, without NADPH and in conditions of RET produced 0.97 ± 0.07 nmol NO. min-1. mg protein-1. Rotenone inhibited NO production supported by RET measured in ETPH and in coupled mitochondria, but did not reduce the activity of recombinant nNOS, indicating that the inhibitory effect of rotenone on NO production is due to an electron flow inhibition and not to a direct action on mtNOS structure. NO production sustained by RET corresponds to 20% of the total amount of NO released from heart coupled mitochondria. A mitochondrial fraction enriched in complex I produced 1.7 ± 0.2 nmol NO. min-1. mg protein-1 and reacted with anti 75 kDa complex I subunit and anti-nNOS antibodies, suggesting that complex I and mtNOS are located contiguously. These data show that mitochondrial NO production can be supported by RET, and suggest that mtNOS is next to complex I, reaffirming the idea of a functional association between these proteins.","palabras_clave":"COMPLEX I|INSIDE-OUT PARTICLES|MITOCHONDRIAL NITRIC OXIDE SYNTHASE|NITRIC OXIDE|REVERSE ELECTRON TRANSFER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6694,"idioma_id":5,"titulo":"Biología, Ecología, Paleontología y filogenia de nuestra Tortuga chaqueña (Chelonoidis chilensis). Pequeño pariente de las tortugas terrestres de las islas galápagos.","resumen":"La tortuga Chelonoidis chilensis es muy popular en nuestro país y en el mundo. Es también conocida como napota GanaGat (lengua Qom l'aqtaqa), chitaní (lengua Wichi), karumbe (Guaraní), Charapa (lengua Quechua) por los pueblos originarios de Argentina.  En América del Norte y gran parte de Europa es conocida como  o (tortuga chaqueña). María Elena Walsh le dedicó una canción llamada ?Manuelita? que inspiró a la realización de una película animada que fue llevada al cine por García Ferré. Las tortugas Chelonoidis chilensis no solamente se encuentran distribuidas en Argentina, también se encuentran en Bolivia y Paraguay. Esta especie habita las regiones fitogeográficas del Chaco Seco y Monte con diferencias morfológicas y genéticas (cariotipo) importantes entre las poblaciones que habitan el Chaco Seco y el Monte. Actualmente, el Chaco Seco y el Monte se ven afectados por la actividad humana que repercute severamente en las poblaciones de estas tortugas. El mascotismo y algunos mitos que existen en torno a las tortugas terrestres, ponen en peligro a la especie. El registro fósil de esta tortuga chaqueña es escaso pero muy importante porque  indica que Chelonoidis chilensis está en nuestro territorio desde hace un millón de años. Es decir, que Ch. chilensis convivió con fauna extinta como perezosos gigantes, gliptodontes, tigres dientes de sable, elefantes y caballos sudamericanos y tortugas terrestres gigantes, todas formas extintas. En América del Sur, Chelonoidis chilensis es la más pequeña de las especies agrupadas en el género Chelonoidis (del griego Chelonos= tortuga) que reúne a la tortuga de patas rojas Ch. carbonaria, la tortuga de patas amarillas Ch. denticulata y las tortugas terrestres gigantes de las Islas Galápagos. Más sorprendente es que evidencias morfológicas y genéticas dan como resultado que dentro del género Chelonoidis la tortugas Ch. chilensis son los parientes más cercanos de las tortugas terrestres gigantes de las Islas Galápagos.","palabras_clave":"Biologia y Paleontología|Testudinidae|Chelonoidis chilensis|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6695,"idioma_id":4,"titulo":"Dynamic global sensitivity analysis in bioreactor networks for bioethanol production","resumen":"Dynamic global sensitivity analysis (GSA) was performed for three different dynamic bioreactor models of increasing complexity: a fermenter for bioethanol production, a bioreactors network, where two types of bioreactors were considered: aerobic for biomass production and anaerobic for bioethanol production and a co-fermenter bioreactor, to identify the parameters that most contribute to uncertainty in model outputs. Sobol´s method was used to calculate time profiles for sensitivity indices. Numerical results have shown the time-variant influence of uncertain parameters on model variables. Most influential model parameters have been determined. For the model of the bioethanol fermenter, &#956;max (maximum growth rate) and Ks (half-saturation constant) are the parameters with largest contribution to model variables uncertainty; in the bioreactors network, the most influential parameter is &#956;max,1 (maximum growth rate in bioreactor 1); whereas &#955; (glucose-to-total sugars concentration ratio in the feed) is the most influential parameter over all model variables in the co-fermentation bioreactor.","palabras_clave":"DYNAMIC GLOBAL SENSITIVITY ANALYSIS|BIOREACTOR NETWORKS|BIOETHANOL PRODUCTION|CO-FERMENTATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6696,"idioma_id":4,"titulo":"Title: 'Striking modification of hippocampal GluN1 and GluN2A NMDA receptor subunits during long-term memory early consolidation in rats'","resumen":"SummaryWe previously showed that hippocampal GluN1 and GluN2A NMDA receptor subunits significantly increased in 2 months old rats following habituation to a novel open field (OF), while GluN2B remained unchanged. Here we showed that 1, 2 and 3 months old rats trained 5min in the OF, showed higher hippocampal GluN1 and GluN2A levels 70-75 minutes later, than animals euthanized immediately after that session, indicating that the subunits rise is a general feature for young rats. In other structures as prefrontal cortex (PFC) and amygdala, no significant changes were found. GluN1 and GluN2A increased in the hippocampus though not in PFC, 70 minutes after new object recognition (NOR) training session; and there were not changes in both structures after test session, 24h later. Although the raise is relatively long-lasting (1½ to 4h), it is transient, strengthened the idea of a putative physiological role. Hippocampal changes overlapped with memory early consolidation period suggesting a possible relationship between them. However, those rats had explored the same OF in 3 consecutive days and the only difference in the 4th day was the presence of two similar objects. Hence, we suggest that an enough relevant change in the environment would elicit significant changes in ?recognition\/ habituation representation?, which would involve subunits modification. In mature primary cultures of hippocampal neurons there was an increase in GluN1 and GluN2A following chemical plasticity induction, reaching a maximum at 75 minutes and returning to control levels 90 minutes later, in bodies and dendrites, showing a close related regulation. GluN2A increase depends on both local translation and preexisting transported GluN2A-NMDAR in similar proportions. These results support the idea that NMDAR subunits change would be related to spatial learning\/memory and hence, at that time interval only happens in the hippocampus where an environment representation\/map is being formed, leading to a LTM. Our in vivo and in vitro results suggest that: 1) the observed hippocampal subunits raise appears structure-specific, at least within that timing; 2) this phenomena is preserved along ages in young rats; 3) it could be related to early consolidation of new spatial information leading to LTM.","palabras_clave":"NMDA receptors|hippocampus|learning and memory|plastic changes"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6697,"idioma_id":5,"titulo":"Conflicto sociocognitivo e intersubjetividad: Análisis de las interacciones verbales en situaciones de aprendizaje colaborativo","resumen":"El presente trabajo tiene como objetivo estudiar el proceso de construcciónsociocognitiva buscando identificar específicamente, en situaciones decolaboración, la presencia de diferentes tipos de conflicto: puramente social ono-cognitivo (CS), sociocognitivo implícito (CSCi) y sociocognitivoexplícito (CSCe). Con este fin, se constituyeron dos grupos de 12 díadas,cada uno pertenecientes a dos disciplinas diferentes: Psicología yFísica. Estas díadas debían aprender colaborativamente un materialacadémico específico relativo a la temática de sus respectivas carreras.Las interacciones fueron grabadas, lo que permitió realizar un análisisexhaustivo de la verbalización. El análisis se centró en identificar alinterior de la discursividad de pares la presencia de estos tres tipos deconflicto, tratando de detectar posibles diferencias entre ambas epistemes. Enrelación a Psicología, los resultados muestran que pueden distinguirse dos grupos de díadas: uno con predominio del CS en desmedro de la intersubjetividad cognitiva, y otro con predominio del CSCi por sobre la regulación puramente social (CS). En comparación con dicha episteme, en Física no existe tal sub-tipología sino que el patrón es relativamente uniformeen todas las díadas, con un predominio del CSCi, un decrecimiento del CS y unaumento CSCe..","palabras_clave":"Conflicto social|Conflicto sociocognitivo|Interacción sociocognitiva|Intersubjetividad"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6700,"idioma_id":4,"titulo":"Cardiac-specific overexpression of thioredoxin 1 attenuates mitochondrial and myocardial dysfunction in septic mice","resumen":"Sepsis-induced myocardial dysfunction is associated with increased oxidative stress and mitochondrial dysfunction. Current evidence suggests a protective role of thioredoxin-1 (Trx1) in the pathogenesis of cardiovascular diseases. However, it is unknown yet a putative role of Trx1 in sepsis-induced myocardial dysfunction, in which oxidative stress is an underlying cause. Transgenic male mice with Trx1 cardiac-specific overexpression (Trx1-Tg) and its wild-type control (wt) were subjected to cecal ligation and puncture or sham surgery. After 6, 18, and 24 h, cardiac contractility, antioxidant enzymes, protein oxidation, and mitochondrial function were evaluated. Trx1 overexpression improved the average life expectancy (Trx1-Tg: 36, wt: 28 h; p = 0.0204). Sepsis induced a decrease in left ventricular developed pressure in both groups, while the contractile reserve, estimated as the response to &#946;-adrenergic stimulus, was higher in Trx1-Tg in relation to wt, after 6 h of the procedure. Trx1 overexpression attenuated complex I inhibition, protein carbonylation, and loss of membrane potential, and preserved Mn superoxide dismutase activity at 24 h. Ultrastructural alterations in mitochondrial cristae were accompanied by reduced optic atrophy 1 (OPA1) fusion protein, and activation of dynamin-related protein 1 (Drp1) (fission protein) in wt mice at 24 h, suggesting mitochondrial fusion\/fission imbalance. PGC-1&#945; gene expression showed a 2.5-fold increase in Trx1-Tg at 24 h, suggesting mitochondrial biogenesis induction. Autophagy, demonstrated by electron microscopy and increased LC3-II\/LC3-I ratio, was observed earlier in Trx1-Tg. In conclusion, Trx1 overexpression extends antioxidant protection, attenuates mitochondrial damage, and activates mitochondrial turnover (mitophagy and biogenesis), preserves contractile reserve and prolongs survival during sepsis.","palabras_clave":"THIOREDOXIN|SEPSIS| MITOCHONDRIAL DYNAMICS|MITOCONDRIAL BIOGENESIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6701,"idioma_id":4,"titulo":"Analytical study of coherence in seeded modulation instability","resumen":"We derive analytical expressions for the coherence in the onset of modulation instability, in excellent agreement with thorough numerical simulations. As usual, we start by a linear perturbation analysis, where broadband noise is added to a cw pump; then, we investigate the effect of adding a deterministic seed to the cw pump, a case of singular interest as it is commonly encountered in parametric amplification schemes. Results for the dependence of coherence on parameters such as fiber type, pump power, propagated distance, and seed signal-to-noise ratio are presented. Finally, we show the importance of including higher-order linear and nonlinear dispersion when looking at longer-wavelength regions (mid IR).","palabras_clave":"MODULATION INSTABILITY|COHERENCE|NONLINEAR OPTICS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6702,"idioma_id":4,"titulo":"Antimicrobial Activity of Lactococcus and Lactobacillus Species Isolated from Raw Goat Milk. San Luis Argentina","resumen":"The goat milk and their products are used to human consumption with health benefits. The lactic acid bacteria (LAB) have ability to produce bacteriocins that inhibit the growth of spoilage microorganisms or pathogens present in food. In this study, bacteriocins-producing strains were isolated from goat milk samples. The isolates were able to inhibit the growth of Listeria monocytogenes ATCC 74902, Enterococcus faecalis ATCC 29212, Escherichia coli and\/or Pseudomonas aeruginosa strains with an average inhibition halo between 8 and 12 mm.  The strains were identified as Lactococcus lactis ssp lactis 2 (gmSL3, gmSL9, gmSL11, gmSL12, gmSL15 and gmSL40), Lactobacillus fermentum (gmSL28), Lactobacillus paracasei ssp paracasei 1 (gmSL5, gmSL20 and gmSL23). The antimicrobial substances showed high stability at temperatures and\/or pH extremes. The tested strains inhibit the growth of more than one pathogen, of both bacteria Gram (+) and Gram (-).  By RT-PCR, one band of 136 bp was found, whose size corresponds to Nisin A.","palabras_clave":"antimicrobial peptides|bacteriocins|Lactic Acid Bacteria (LAB)|raw goat milk"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6703,"idioma_id":4,"titulo":"Key management practices to prevent high infestation levels of Varroa destructor in honey bee colonies at the beginning of the honey yield season","resumen":"Varroa destructor is considered one of the main threats to worldwide apiculture causing a variety of physiological effects at individual and colony level. Also, Varroa mites are often associated with several honey bee viruses presence. Relatively low levels of Varroa during the spring, at the beginning of the honey yield season, can have a significant economic impact on honey production and colony health. Winter treatments against Varroa and certain management practices may delay mite population growth during following spring and summer improving colonies performance during the honey yield season. The aim of this study was to identify risk factors associated with the presence of Varroa destructor in late spring in apiaries from temperate climate. A longitudinal study was carried out in 48 apiaries, randomly selected to evaluate V. destructor infestation level throughout the year. The percentage of infestation with V. destructor was assessed four times during one year and the beekeepers answered a survey concerning all management practices applied in the colonies. We used a generalized linear mixed model to determine association between risk of achieving 2% infestation on adult bees at the beginning of the honey yield season and all potential explanatory variables. The complete dataset was scanned to identify colonies clusters with a higher probability of achieving damage thresholds throughout the year. Colonies that achieved &#8805;2% of infestation with V. destructor during spring were owned by less experienced beekeepers. Moreover, as Varroa populations increase exponentially during spring and summer, if the spring sampling time is later this growth remains unobserved. Monitoring and winter treatment can be critical for controlling mite population during the honey production cycle. Spatial distribution of colonies with a higher risk of achieving high Varroa levels seems to be better explained by management practices than a geographical condition.","palabras_clave":"VARROA DESTRUCTOR|APIS MELLIFERA|WINTER  TREATMENT|LONGITUDINAL STUDIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6704,"idioma_id":4,"titulo":"Molecular characterization of a clinical Haemophilus parainfluenzae isolate with cefotaxime resistance and decreased susceptibility to fluoroquinolones","resumen":"We report an H. parainfluenzae clinical isolate resistant to cefotaxime and with decreased susceptibility to ciprofloxacinrecovered from a patient with cystic fibrosis. The isolate had elevated MICs of ampicillin (256 mg\/L), amoxicillin-clavulanate(8 mg\/L), cefuroxime (8 mg\/L) and cefotaxime (4 mg\/L), and showed a &#946;-lactamase-producing amoxicillin-clavulanicacid-resistant (BLPACR) phenotype. A blaTEM-1 plus five amino acid substitutions in the PBP3 were found:Ser385Thr, Val511Ala, Ile519Val, Asn526Lys and Asp551Leu. MIC of ciprofloxacin was 0.5 mg\/L, and substitutions ingyrA (Ser84Tyr) and parC (Ser84Phe) genes were detected.","palabras_clave":"HAEMOPHILUS|CEFOTAXIME|FLUOROQUINOLONE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6705,"idioma_id":4,"titulo":"Effects of Spatial Sampling Interval on Roughness Parameters and Microwave Backscatter over Agricultural Soil Surfaces","resumen":"Abstract: The spatial sampling interval, as related to the ability to digitize a soil profile with a certain number of features per unit length, depends on the profiling technique itself. From a variety of profiling techniques, roughness parameters are estimated at different sampling intervals. Since soilprofiles have continuous spectral components, it is clear that roughness parameters are influenced by the sampling interval of the measurement device employed. In this work, we contributed to answer which sampling interval the profiles needed to be measured at to accurately account for the microwave response of agricultural surfaces. For this purpose, a 2-D laser profiler wasbuilt and used to measure surface soil roughness at field scale over agricultural sites in Argentina. Sampling intervals ranged from large (50 mm) to small ones (1 mm), with several intermediate values. Large- and intermediate-sampling-interval profiles were synthetically derived from nominal, 1 mm ones. With these data, the effect of sampling-interval-dependent roughness parameters on backscatter response was assessed using the theoretical backscatter model IEM2M. Simulations demonstrated that variations of roughness parameters depended on the working wavelength and was less important at L-band than at C- or X-band. In any case, an underestimation of the backscattering coefficient of about 1-4 dB was observed at larger sampling intervals. As a general rule a sampling interval of15 mm can be recommended for L-band and 5 mm for C-band","palabras_clave":"SURFACE SOIL ROUGHNESS|LASER PROFILER|HEIGHT STANDARD DEVIATION| CORRELATION LENGTH| RADAR APPLICATIONS| SCATTERING MODELS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6706,"idioma_id":5,"titulo":"Anidamiento de paloma torcaza (Zenaida auriculata) en rastrojos de sorgo","resumen":"La paloma torcaza (Zenaida auriculata) es una de las especies de aves más co-munes y abundantes de Argentina, con gran capacidad de adaptación a los am-bientes modificados por el hombre. Un aspecto clave del éxito de esta especie es su capacidad para anidar de manera colonial en remanentes de bosque en paisajes agrícolas. Este factor ha contribuido significativamente al crecimiento poblacional experimentado por la especie en las últimas décadas. Se presenta el hallazgo de una nidada colonial en rastrojos de sorgo. En octubre de 2013 se localizó una colonia de anidamiento de paloma torcaza en un lote con rastrojos de sorgo. En dicho lote, se realizaron 5 transectas de faja. Se obtuvo un promedio de 0,89 nidos (±0,19) por m2 y 1,79 (±0,17), huevos por nido. La mayoría (93,4 %) de los huevos estaba sano y el resto (6,6 %) mostraba signos de depredación. Éste sería el primer registro publicado de anidamiento colonial de torcaza en rastrojos de cultivo de sorgo. Establecer la frecuencia de este comportamiento de anidamiento a escala regional y estimar el impacto que podría tener sobre el crecimiento poblacional de esta especie sería importante para comprender, de manera más cabal, los aumentos poblacionales experimentados por esta paloma en los últimos años.","palabras_clave":"ADAPTACION|AVES|CULTIVO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6707,"idioma_id":5,"titulo":"¿Eligen las palomas y cotorras los lotes de soja (Glycine max) en emergencia? Un caso de estudio en agroecosistemas de Entre Ríos, Argentina","resumen":"Las palomas y cotorras pueden resultar perjudiciales para cultivos anuales en algunas regiones de Sudamérica. En este estudio analizamos patrones de uso de hábitat de las palomas torcaza (Zenaida auriculata), manchada (Patagioenas maculosa) y picazuró (P. picazuro) y la cotorra (Myiopsitta monachus) durante la primera emergencia del cultivo de soja en Entre Ríos, Argentina. Se estimó el uso y la disponibilidad de hábitats y se calculó la selección mediante intervalos de confianza de Bonferroni. Ninguna de las 4 especies seleccionó el cultivo de soja. La paloma torcaza seleccionó rastrojos de trigo y colza, las palomas picazuró y manchada seleccionaron hábitats con árboles nativos e introducidos; finalmente, la cotorra común seleccionó hábitats con árboles introducidos. Esto reforzaría la hipótesis de que estas especies solo seleccionaron lotes con cotiledones de soja cuando existe escasez de granos en el ambiente. Asumiendo que esta hipótesis sea verdadera, una alternativa para disminuir daños por aves en soja en emergencia sería disuadirlas de utilizar los lotes de soja, ofreciendo granos para atraerlas a otros sitios y evitar que dañen el cultivo. Esta disuasión podría lograrse haciendo coincidir el espacio y la temporalidad de la siembra de soja con la cosecha de cultivos invernales, o bien ofreciendo granos como atrayentes para las aves.","palabras_clave":"DAÑO POR AVES|SELECCION DE HABITAT|USO DE HABITAT|CULTIVOS|RASTROJOS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6708,"idioma_id":4,"titulo":"Bordetella pertussis modulates human macrophage defense gene expression","resumen":"Bordetella pertussis, the etiological agent of whooping cough, still causes outbreaks. We recently found evidence that B.pertussis can survive and even replicate inside human macrophages, indicating that this host cell might serve as a niche forpersistence. In this work, we examined the interaction of B. pertussis with a human monocyte cell line (THP-1) thatdifferentiates into macrophages in culture in order to investigate the host cell response to the infection and themechanisms that promote that intracellular survival. To that end, we investigated the expression profile of a selectednumber of genes involved in cellular bactericidal activity and the inflammatory response during the early and late phasesof infection. The bactericidal and inflammatory response of infected macrophages was progressively downregulated, whilethe number of THP-1 cells heavily loaded with live bacteria increased over time postinfection. Two of the main toxins of B.pertussis, pertussis toxin (Ptx) and adenylate cyclase (CyaA), were found to be involved in manipulating the host cellresponse. Therefore, failure to express either toxin proved detrimental to the development of intracellular infections bythose bacteria. Taken together, these results support the relevance of host defense gene manipulation to the outcome ofthe interaction between B. pertussis and macrophages.","palabras_clave":"Bordetella pertussis|host cell defense response|intracellular survival|adenylate cyclase|pertussis toxin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6709,"idioma_id":4,"titulo":"Maternal Triclosan consumption alters the appetite regulatory network on Wistar rat offspring and predispose to metabolic syndrome in the adulthood","resumen":"Abstract. The objectives of this study were to evaluate the effects of maternal oral exposure to the antibacterial Triclosan (TCS) during gestation and lactation on the metabolic status of the adult offspring and on the expression of main genes controlling the appetite regulatory network. Pregnant rats were fed ad-libitum with ground food + TCS (1 mg\/kg) from day 14 of gestation to day 20 of lactation (n=3) or ground food (n=3). After litter reduction, 12 males and 12 females born from the TCS exposed rats (TCS, n=24) or not (Control, n=24) were used to evaluate monthly body weight, food intake, plasma levels of cholesterol, glucose and triglycerides, and the hypothalamic mRNA expression of agouti-related protein (Agrp), neuropeptide Y (Npy) and propiomelanocortin (Pomc). Body weight for rats in the TCS group was 12.5% heavier for males at 4 months (p<0.001) and 19% heavier for females at 8 months (p=0.01). Food intake was significantly higher for rats in the TCS group at 5 months of age (p<0.01). Cholesterol and glucose levels were significantly higher for rats in the TCS group at 8 months (p<0.05). mRNA expression of Npy and Agrp were significantly increased in hypothalami of rats in the TCS group at 2 months for males or 8 months for females (p<0.05). In conclusion, low doses of oral TCS consumption by the pregnant and lactating dam increase the hypothalamic expression of the orexigenic neuropeptides Npy and Agrp in the offspring and alter their metabolic status during adulthood, resembling development of the metabolic syndrome.","palabras_clave":"Triclosan|Sindrome metabólico|Hipertension"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6710,"idioma_id":4,"titulo":"Automatic Methodology for Mapping of Coastal Zones in Video Sequences","resumen":"Gathering precise and detailed geomorphological and dynamic information of coastal processes is increasingly required for environmental studies and coastal management policies as well. Traditional methods for in situ measurements, or remote sensing monitoring by satellites or airbone imagery, impose limitations and tradeoffs between image quality, operational costs, availability, and negative environmental effects. These limitations and tradeoffs restrict the kind of environmental studies that can be undertaken, specifically when a high spatial and temporal resolution is required over wide geographical areas. In the last decades, video monitoring systems have demonstrated to be a cost-effective alternative for this and other similar purposes. Notwithstanding that, video processing is not fully mature in the context of environmental monitoring in general, and, thus, most of the past and current efforts have been developed in an ad hoc basis. This has the drawback that most available solutions are hardly useful in contexts different from their original setup. In this work we develop an autonomous application for geographic feature extraction and recognition in coastal videos. Specifically, we address the classification and feature measurement of multiple beach zones, a topic addressed to a lesser extent by other projects. The system is designed to be deployed in inexpensive, off-the-shelf hardware, and open source software development frameworks, in a way such that the results can be easily replicated by other research groups. The initial setup and calibration requires very simple supervision, thus allowing the system to be used in a variety of coastal environments.","palabras_clave":"Image Processing|Coastal analysis software|Environmental monitoring|Beach management"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6712,"idioma_id":4,"titulo":"Monitoring of chlorsulfuron in biological fluids and water samples by molecular fluorescence using rhodamine B as fluorophore","resumen":"A new simple methodology is proposed for chlorsufuron (CS) traces quantification based upon enhancement of rhodamine B (RhB) fluorescent signal. Experimental variables that influence fluorimetric sensitivity have been studied and optimized. The zeroth order regression calibration was linear from 0.866 to 35.800 µg L&#8722;1 CS, with a correlation coefficient of 0.99. At optimal experimental conditions, a limit of detection of 0.259 µg L&#8722;1 and a limit of quantification of 0.866 µg L&#8722;1 were obtained. The method showed good sensitivity and adequate selectivity and was applied to the determination of trace amounts of CS in plasma, serum and water samples with satisfactory results analyzed by ANOVA test. The proposed methodology represents an alternative to traditional chromatographic techniques for CS monitoring in complex samples, using an accessible instrument in control laboratories.","palabras_clave":"Chlorsulfuron monitoring|Rhodamine B|Molecular fluorescence|Biological and Water samples"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6713,"idioma_id":4,"titulo":"A high resolution HI study  towards the supernova remnant Puppis A and its environments","resumen":"We observed the supernova remnant (SNR) Puppis A in the 21 cm line with the Australia Telescope Compact Array with the aim of determining the systemic velocity and, hence, the corresponding kinematic distance. For the compact, background sources in the field, we obtain absorption spectra by applying two methods: (a) subtracting profiles on- and off-source towards continuum emission, and (b) filtering short spacial frequencies in the Fourier plane to remove large scale emission. One of the brightest features to the East of the shell of Puppis A was found to be a background source, probably extragalactic. Removing the contribution from this and the previously known unrelated sources, the systemic velocity of Puppis A turns out to be limited between 8 and 12 km\/s, which places this source at a distance of 1.3 +\/- 0.3 kpc. From the combined images that include both single dish and interferometric data, we analyze the distribution of the interstellar hydrogen. We suggest that an ellipsoidal ring at v ~ +8 km\/s could be the relic of a bubble blown by the progenitor of Puppis A, provided the distance is <1.2 kpc. The main consequences of the new systemic velocity and distance as compared with previous publications (v = +16 km\/s  and d = 2.2 kpc) are the absence of a dense interacting cloud to the East to explain the morphology, and the decrease of the shell size and the neutron star velocity, which are now in better agreement with statistical values.","palabras_clave":"ISM: SUPERNOVA REMNANTS|ISM: INDIVIDUAL OBJECTS: PUPPIS A|INTERSTELLAR MEDIUM|NEUTRAL HYDROGEN"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6714,"idioma_id":4,"titulo":"Synthesis and Characterization of Polyurethane Rigid Foams from Soybean Oil-Based Polyol and Glycerol","resumen":"Mixtures of biomass-derived polyols were used to synthesize rigid polyurethane (PU) foams. A commercialpolymerized methylene diphenyl diisocyanate (pMDI) was used as crosslinker, and distilled water servedas foaming agent. The morphology and mechanical properties of foams with different glycerol and watercontents were compared in order to evaluate the most suitable formulations. The rigid foams with higherwater contents had larger and more anisotropic cells, explaining their lower density. Compressive moduliranged from about 2.5 MPa to above 20 MPa and collapse stresses from 55 kPa to more than 1 MPa fordensities between 54 and 143 kg\/m3. Densification strain did not depend on the density or on the compositionof the polymeric matrix. Moreover, results shown herein demonstrate that an increase in the glycerol contentleads to an increase in the required pMDI for the synthesis of the PU, but with a negligible change in themechanical properties of the prepared foams.","palabras_clave":"POLYURETHANE FOAMS|HYDROXYLATED SOYBEAN OIL|GLYCEROL|MECHANCIAL PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6715,"idioma_id":4,"titulo":"Increase in pertussis cases along with high prevalence of two emerging genotypes of Bordetella pertussis in Perú, 2012","resumen":"AbstractBACKGROUND: As has occurred in many regions worldwide, in 2012 the incidence of pertussis increased in Perú. This epidemiologic situation has been associated with a waning vaccine-induced immunity and the adaptation of Bordetella pertussis to vaccine-induced immunity along with improved diagnostic methods.METHODS: The study comprised a total of 840 pertussis-suspected cases reported in Perú during 2012. We summarize here the distribution of pertussis cases according to age and immunization status along with the immunization-coverage rate. Laboratory diagnosis was performed by culture test and real-time polymerase-chain reaction (PCR). B. pertussis bacteria recovered from infected patients were characterized by pulsed-field gel electrophoresis (PFGE), and the DNA sequencing of the pertussis-toxin (promoter and subunit A), pertactin, and fimbriae (fim2 and fim3) genes.RESULTS: From the total pertussis-suspected cases, 191 (22.7&nbsp;%) infections were confirmed by real-time PCR and 18 through cultivation of B. pertussis (2.1&nbsp;%), while one infection of B. parapertussis (0.11&nbsp;%) was also detected by culture. Pertussis was significantly higher in patients that had had 0-3 vaccine doses (pentavalent vaccine alone) than in those who had had 4-5 vaccine doses (pentavalent plus DwPT boosters) at 94.3 vs. 5.7&nbsp;%, respectively (p&#8201;&lt;&#8201;0.00001). The relative risk (RR) for patients with 4-5 doses compared to those with fewer than 4 doses or no dose was 0.23 (95&nbsp;% Confidence Interval: 0.11-0.44), while the vaccine effectiveness was 77&nbsp;% and coverage 50.5&nbsp;%. Genetic analysis of B. pertussis isolates from different Peruvian regions detected two clonal groups as identified by PFGE. Those two groups corresponded to the B. pertussis genotypes emerging worldwide ptxP3-ptxA1-prn2 or 9-fim3-1 and ptxP3-ptxA1-prn2 or 9-fim3-2.CONCLUSIONS: Two emerging B. pertussis genotypes similar to isolates involved in worldwide epidemics were detected in Perú. Low vaccine coverage (&lt;50&nbsp;%) and genetic divergence between the vaccine-producing strain and the local isolates could contribute to this pertussal epidemic.","palabras_clave":"Pertussis|Bordetella pertussis|Genotype|Pulsed-field gel electrophoresis|Perú"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6716,"idioma_id":4,"titulo":"A new elasmosaurid specimen from the upper Maastrichtian of Antarctica: new evidence of a monophyletic group of Weddellian elasmosaurids","resumen":"A new fossil elasmosaurid specimen, MLP 15-I-7?48, from the upper Maastrichtian Sandwich Bluff Member of the López de Bertodano Formation, Vega Island, Archipelago James Ross, Antarctica, is described. The fossil is a well-preserved anterior limb, which shares with 10 Vegasaurus molyi from the upper Maastrichtian of Antarctica, a concave anterior margin of the humeral shaft, and with Vegasaurus molyi and Aphrosaurus furlongi from the upper Maastrichtian of California, a well-defined depression on the anterior margin of the ventral surface of the humeral shaft. A phylogenetic analysis recovered MLP 15-I-7?48 as sister group of the lower Maastrichtian Vegasaurus molyi within a new cladenominated as Weddellonectia: Kawanectes lafquenianum {[Vegasaurus molyi; MLP 15-I-7?48] [Morenosaurus stocki (Aristonectine)]}. This indicates that the previously proposed faunal turnover between the early and late Maastrichtian Weddellian marine reptile fauna, did not severely affect the non-aristonectine elasmosaurids. Additionally, other taxa previously considered evidence of a faunal turnover are re-evaluated.","palabras_clave":"Weddellian|Elasmosauridae|Biogeographic Province|Aristonectinae|Antarctica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6717,"idioma_id":4,"titulo":"Biosorption of aluminum through the use of non-viable biomass of Pseudomonas putida","resumen":"Living and non-living biomass of Pseudomonas putida A (ATCC 12633) was used as biosorbent for the removing of Al3+ from aqueous solutions. The process was stable with time, efficient at pH 4.3 and between 15 °C and 42 °C. Two isotherms models were applied to describe the interaction between the biosorbent and Al3+. Non-living biomass of P. putida A (ATCC 12633) was found to be the most efficient at adsorbing Al3+ with a maximum sorption capacity of 0.55 mg Al3+\/gr adsorbent and with 36 × 105 binding sites of Al3+\/microorganisms. Infrared spectroscopy analysis shows that the biosorbent present some vibrational band of functional groups that change in presence of Al3+: hydroxyl, carboxyl and phosphate. Considering that Al3+ binds to the phosphate group of phosphatidylcholine, non-viable biomass of P. putida PB01 (mutant lacking phosphatidylcholine) was used. Aluminum adsorption of the parental strain was 30 times higher than values registered in P. putida PB01 (36 × 105 sites\/microorganism vs 1.2 × 105 sites\/microorganism, respectively). This result evidenced that the absence of phosphatidylcholine significantly affected the availability of the binding sites and consequently the efficiency of the biomass to adsorb Al3+.","palabras_clave":"Biosoption|Toxic metals|Aluminium|Pseudomonas putida"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6718,"idioma_id":4,"titulo":"Content of cardiolipi n of the membrane and sensitivit y to cationic surfactants in Pseudomonas putida","resumen":"Aims: To establish the role of cardiolipin of the membrane in response to the presence of tetradecyltrimethylammonium in Pseudomonas putida A (ATCC 12633).Methods and Results: Two ORFs of Ps. putida A (ATCC 12633), which in Ps. putida KT2440 encode the putative cardiolipin synthase genes cls and cls2, were cloned, sequenced and mutated. Only the double mutant, lacking cls and cls2, showed a reduction of the cardiolipin content, 83% lower than the amount produced by the wild-type. Accompanying this change was a 40% decrease in the content of unsaturated fatty acid, consequently, the membrane of the mutant was more rigid than the one of the parental strain, as observed using fluorescence polarization techniques. The mutant strain showed reduced viability in presence of tetradecyltrimethylammonium.The incorporation of exogenous cardiolipin into its membrane relieved sensitivity to the cationic detergent.Conclusions: Ps. Putida cells with low levels of cardiolipin die in presence of tetradecyltrimethylammonium, because they cannot counter the fluidizing effect of the cationic surfactant.Significance and Impact of the Study: The modification in the membrane phospholipids composition allows knowing the adaptation strategy of Ps. putida when these bacteria are exposed to cationic surfactant.","palabras_clave":"PSEUDOMONAS PUTIDA|CARDIOLIPIN |CATIONIC SURFACTANT|CARDIOLIPIN SYNTHASE GENES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6719,"idioma_id":4,"titulo":"La\/Ba-based cobaltites as IT-SOFC cathodes: a discussion about the effect of crystal structure and microstructure on the O2-reduction reaction","resumen":"This work discusses the effect of the crystal structure and microstructure on the electrocatalytic activity of Lanthanum-Barium cobaltite (LBC), evaluatedas IT-SOFC cathode. Two systems with similar microstructures (particle sizes dp 1?5 mm) are used to compare the effect of the crystal structure: the cubic La0.5Ba0.5CoO3-d with La\/Ba cations randomly distributed, and a tetragonal LaBaCo2O6-d with La\/Ba layered distribution. In addition, the effect of microstructure is studied by using a newsol-gel route which allows obtaining LaBaCo2O6-d with smallest particle size (dp500 nm). The electrode reaction isstudied by electrochemical impedance spectroscopy (EIS) as a function of temperature (T) and oxygen partial pressure (pO2). The electrode polarization resistance (RC,P) presents two contributions, a low frequency (RLF) and a high frequency Gerischer-resistance (RG). On one side, RLF, associated to the O2-gas diffusion across the gas layer boundary, presents low activation energy (Ea) and is proportional to pO2 1, presenting the same values regardless the microstructure.On the other side, RG is in agreement with anO2-reduction mechanismco-limited by O-surface exchange and O-bulk diffusion. RG is stronglyinfluenced by structure (trough the diffusion coefficient DV) and microstructure (inversely proportional to dp). Besides, RG decreases by reducing dp and by modifying the La\/Ba ordering from layered torandomly distributed.","palabras_clave":"IT-SOFC|PEROVSKITE|CATHODE|O2 REDUCTION REACTION|LBC"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6720,"idioma_id":4,"titulo":"Modeling of RAFT Polymerization Processes using an Efficient Monte Carlo Algorithm in Julia","resumen":"A kinetic Monte Carlo model of a RAFT process is presented. The algorithm has been developed and implemented in Julia for the three main RAFT theories under current discussion (Slow Fragmentation, Intermediate Radical Termination and Intermediate Radical Termination with Oligomers). Julia is a modern programming language designed to achieve high-performance in numerical and scientific computing. Thanks to a careful optimization of the code, it is possible to simulate a RAFT reaction scheme in short computing times for any of the three theories. The code is benchmarked against other programming languages (MATLAB, Python, FORTRAN and C), showing that Julia presents advantages for this particular system. The model offers an efficient method for predicting average properties and molecular weight distributions of the polymer species, including the bivariate MWD of the intermediate 2-arm adduct. The proposed model can also be employed to obtain additional detailed information regarding the polymer microstructure at any reaction time.","palabras_clave":"RAFT POLYMERIZATION|MATHEMATICAL MODEL|MONTE CARLO|JULIA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6721,"idioma_id":4,"titulo":"Improved Numerical Inversion Methods for the Recovery of Bivariate Distributions of Polymer Properties from 2D Probability Generating Function Domains","resumen":"The 2D probability-generating function technique is a powerful method for modeling bivariate distributions of polymer properties. It is based on the transformation of bivariate population balance equations using 2D probability generating functions (pgf) followed by a recovery of the distributions from the transform domain by numerical inversion. A key step of this method is the inversion of the pgf transforms. Available numerical inversion methods yield excellent results for pgf transforms of distributions with independent dimensions with similar orders of magnitude, for example bivariate molecular weight distributions in copolymerization systems. However, numerical problems are found for 2D distributions in which the independent dimensions have very different ranges of values, such as the molecular weight distribution-branching distribution in branched polymers. In this work, two new 2D pgf inversion methods are developed, which regard the pgf as a complex variable. The superior accuracy of these innovative methods makes them suitable for recovering any type of bivariate distribution. This enhances the capabilities of the 2D pgf modeling technique for simulation and optimization of polymer processes. An application example of the technique in a polymeric system of industrial interest is presented.","palabras_clave":"MODELING|POLYMERIZATION|BIVARIATE DISTRIBUTION|2d PROBABILITY GENERATING FUNCTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6722,"idioma_id":5,"titulo":"Proceso de implementación del nuevo entorno  virtual de enseñanza y aprendizaje Moodle de  la Facultad de Ciencias Médicas de la UNLP.  Planificación y temas críticos","resumen":"Esteartículo presenta el plan diseñado e implementado en la Facultad de CienciasMédicas de la UNLP (Universidad Nacional de La Plata) para una transición haciaun nuevo entorno virtual de enseñanza y aprendizaje (EVEA), con un rediseño dela interfaz y una migración de los cursos de grado y postgrado. Los pasosfueron: la instalación del programa Moodle (versión 2.7) en un servidor de la Facultad;su organización según la estructura de carreras que ofrece la Facultad; el diseñopersonalizado de la interfaz; la instalación de una lista de usuarios con los listadosoficiales y la transferencia de los cursos al nuevo entorno desde el entornousado anteriormente en la Facultad. Se implementó un diseño adaptativo, con la opciónde utilizar la aplicación Moodle Mobile para el acceso desde dispositivosmóviles. El nuevo entorno (http:\/\/educativa.med.unlp.edu.ar) comenzó autilizarse en el inicio del ciclo lectivo de 2015. Cuenta a ctualmente con 103cursos y 4100 usuarios. Las cátedras están utilizando una variedad deherramientas, las que incluyen varios recursos (etiquetas, archivos, links,carpetas, páginas web) y actividades (foros, glosarios, cuestionarios, entregade tareas, r egistros de asistencia online). Se han realizado además variasactividades de capacitación docente en el uso del EVEAs. El proceso descriptoes un componente para la mejora continua de la enseñanza mediante la tecnologíadigital y puede ser tomado como guí a por otras instituciones que deseenincorporar el uso de EVEAs a sus propuestas educativas.","palabras_clave":"Educación a Distancia|Educación Médica|Entornos Virtuales de Enseñanza y Aprendizaje|Medicina|Moodle|Tecnología Educativa"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6723,"idioma_id":4,"titulo":"Phenolic Compounds in Extracts from Eucalyptus globulus Leaves and Calendula officinalis Flowers","resumen":"Selection of the optimal solvent system for extraction of the phenolics from Eucalyptus globules leaves and Calendula officinalis flowers, determination of the reducing potential and identification of the phenolics in these extracts was performed. The highest content of phenolics was obtained for methanol: water extracts from both sources. All of the Eucalyptus leaf extracts had higher reducing potential than those from the Calendula flowers. Solid-phase purification of the crude extracts removed 57% to 78% of the compounds in the crude extracts. The reducing potential of the purified extracts varied from 0.17 to 2.92 mg caffeic acid\/g dry weight. The extracts from Eucalyptus leaves and Calendula flowers both contained chlorogenic acid, rutin and quercetin 3-glucuronide. Ellagic acid derivatives were identified only in the leaves of Eucalyptus, while beside caffeic acid and salicylic acid, quercetin 3-glucuronide, and pinobanksin 3-acetate was found in the Calendula flower extract for the first time.","palabras_clave":"PHENOLICS|EUCALYPTUS|CALENDULA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6726,"idioma_id":4,"titulo":"Effect of Sodium Chloride Reduction and Wheat Fiber Addition on Chicken Nugget Quality","resumen":"The effect of sodium chloride (NaCl) reduction and wheat fiber (F) addition on chicken nugget quality was analysed. Storage stability of a low sodium formulation was also determined. Chicken nuggets with the addition of sodium chloride (0.5 ? (to) 1.9%) and wheat fiber (0 ? (to) 2%) were prepared. Physico-chemical parameters such as PH, cooking loss, texture, color, fat, protein and moisture were determined. The effect of NaCl and F on these parameters was analyzed using response surface methodology. A sensory test was also conducted by consumers (60) who evaluated overall acceptability. Storage stability of two batches (Batch 1: no F and 1.6% NaCl; Batch 2: 2% F and 0.8% NaCl) was measured by performing TBARS, microbial counts and descriptive sensory test (14 trained judges) for rancid characteristics. Data was analyzed with ANOVA.The reduction of NaCl by 50% and the addition of 2% fiber improved the quality ofchicken nuggets. These chicken nuggets had less cooking loss, tenderer characteristics and a slight decrease in whiteness without affecting consumer acceptability or storage behavior.","palabras_clave":"Chicken nugget|healthier product|reduced sodium|wheat fiber|consumer acceptability|storage behavior"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6727,"idioma_id":4,"titulo":"Statistical Process Control: A Quality Tool for a Venous Thromboembolic Disease Registry","resumen":"Registry Manag. 2016 Summer;43(2):82-6.Statistical Process Control: A Quality Tool for a Venous Thromboembolic Disease Registry.Posadas-Martinez ML, Rojas LP, Vazquez FJ, De Quiros FB, Waisman GD, Giunta DH.AbstractOBJECTIVE:We aim to describe Statistical Control Process as a quality tool for the Institutional Registry of Venous Thromboembolic Disease (IRTD), a registry developed in a community-care tertiary hospital in Buenos Aires, Argentina.METHODS:The IRTD is a prospective cohort. The process of data acquisition began with the creation of a computerized alert generated whenever physicians requested imaging or laboratory study to diagnose venous thromboembolism, which defined eligible patients. The process then followed a structured methodology for patient´s inclusion, evaluation, and posterior data entry. To control this process, process performance indicators were designed to be measured monthly. These included the number of eligible patients, the number of included patients, median time to patient´s evaluation, and percentage of patients lost to evaluation. Control charts were graphed for each indicator.RESULTS:The registry was evaluated in 93 months, where 25,757 patients were reported and 6,798 patients met inclusion criteria. The median time to evaluation was 20 hours (SD, 12) and 7.7% of the total was lost to evaluation. Each indicator presented trends over time, caused by structural changes and improvement cycles, and therefore the central limit suffered inflexions.CONCLUSION:Statistical process control through process performance indicators allowed us to control the performance of the registry over time to detect systematic problems. We postulate that this approach could be reproduced for other clinical registries.","palabras_clave":"trombosis|registro"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6728,"idioma_id":10,"titulo":"Esfera pública e redes socias na Internet: O que é novo no Facebook?","resumen":"Vivemos em sociedades altamente midiatizadas e não somente atravessadas pela ação dos meios de comunicação de massa mas, também, pelas novas tensões que assume o processo de midiatização a partir do desenvolvimento das redes sociais em Internet. Ao reconhecer a natureza ambiental e construtiva dos meios de comunicação de massa é possível, também, considerar a midiatização como modalidade nuclear da construção da esfera pública. Neste contexto, este artigo pergunta sobre os modos onde o funcionamento do Facebook e os discursos aí expostos participam na configuração da esfera pública contemporânea, assim como, ademais, sobre o grau de novidade que mostra dita intervenção a respeito da exercida pelos tradicionais meios de comunicação de massa. Em função de articular as reflexões expostas com alguns dos eixos que tomou o debate teórico lançado pela análise que realizou Habermas sobre a gênese e as transformações estruturais da vida pública, este artigo enfoca nestes três seguintes aspectos: a gestão da visibilidade, da colocação pública ou publicação; o lugar que ocupa o diálogo, a deliberação e o dissenso; a condição múltipla e móvel da esfera pública atual.","palabras_clave":"Esfera Pública|Facebook,|Midiatização|Redes Sociais em Internet."},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6729,"idioma_id":5,"titulo":"Primer registro de Tetranycopsis horridus (Canestrini & Fanzago) en Argentina aso- ciado al avellano  (Corylus avellana)","resumen":"RESUMENEn Argentina, la información sobre los agentes perjudiciales presentes en avellanos es escasa. En la temporada2014-2015, el Instituto Nacional de Tecnología Agropecuaria llevó a cabo el monitoreo en plantacionescomerciales localizadas en el Valle Inferior de Río Negro. En una de ellas se observó un desarrollo anormal dela brotación. Al examinar a campo el material vegetal afectado se relacionó la sintomatología con la actividadalimentaria de un ácaro. Se tomaron muestras y se las envió al Laboratorio de Referencia del Servicio Nacionalde Sanidad y Calidad Agroalimentaria para su análisis. Se confeccionaron preparados microscópicos de23 ejemplares en distintos estados de desarrollo. La determinación de la especie se realizó sobre hembrasadultas mediante claves taxonómicas y bibliografía disponible. El objetivo de este trabajo es reportar en Argentina,por primera vez, la presencia de Tetranycopsis horridus (Canestrini & Fanzago) sobre C. avellana;asimismo, suministrar información sobre la plaga para facilitar el reconocimiento de su sintomatología y laidentificación taxonómica.","palabras_clave":"Avellano|Acaro|Registro|Argentina"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6730,"idioma_id":4,"titulo":"Identification and profiling of microRNAs in two developmental stages of the model cestode parasite Mesocestoides corti","resumen":"MicroRNAs (miRNAs), a class of small non-coding RNAs, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as metabolism and development. The particular developmental characteristics of cestode parasites highlight the importance of studying miRNA gene regulation in these organisms. Here, we performed a comprehensive analysis of miRNAs in two developmental stages of the model cestode Mesocestoides corti. Using a high-throughput sequencing approach, we found transcriptional evidence of 42 miRNA loci in tetrathyridia larvae and strobilated worms. Tetrathyridium and strobilated worm-specific miRNAs were found, as well as differentialy expressed miRNAs between these developmental stages, suggesting miRNA regulation of stage-specific features. Moreover, it was shown that uridylation is a differential mechanism of post-transcriptional modification of M. corti miRNAs. The whole set of M. corti miRNAs represent 33 unique miRNA families, and confirm the remarkable loss of conserved miRNA families within platyhelminth parasites, reflecting their relatively low morphological complexity and high adaptation to parasitism. Overall, the presented results provide a valuable platform to studies aiming to identify and characterize novel miRNA-based molecular mechanisms of post-transcriptional gene regulation in cestodes, necessary for the elucidation of developmental aspects of the complex biology of these parasites.","palabras_clave":"miRNAs|Strobilation|RNA-seq|Differential expression"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6731,"idioma_id":4,"titulo":"Assessing sewage impact in a South-West Atlantic rocky shore intertidal algal community","resumen":"The spatial and seasonal variation of the specific composition and community parameters (abundance, diversity, richness and evenness) of the intertidal algae algal assemblages was studied at four coastal sampling sites, distributed along an environmental gradient from the sewage water outfall of Mar del Plata, Buenos Aires, Argentina. Two of them were located close to the sewage outfall (< 800 m) (impacted area) and the two other were 8 km and 9 km distant (non-impacted area). The algal abundance was monthly analyzed from October 2008 to May 2009. The algal assemblages varied according to the pollution gradient in spring, summer and autumn, being autumn the season where when the higher difference were was observed. C. eramium uruguayense was recognized as an indicator species for the non-impacted areas, while Berkeleya sp. represented an indicative species for the sewage outfall impact. Ulva spp. did not reflect the typical pattern observed for other sewage pollution areas.","palabras_clave":"sewage outfall|coastal pollution|Mar del Plata|algal indicators"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6732,"idioma_id":4,"titulo":"Succession in an intertidal benthic community affected by untreated sewage effluent: A case of study in the SW Atlantic shore","resumen":"A study of benthic succession related with sewage pollution was conducted in a warm-temperate coastal area of Mar del Plata city, Argentina. The effect of nutrient enrichment and starting period of the succession was tested after cleared space in the intertidal rocky shore benthic community. The time of recovery after a disturbance in enriched sites was considerably lower (20?29 weeks) than in non-enriched sites based on the diatom Berkeleya sp. and the polychaete Boccardia proboscidea developed in the early succession stage, and Brachidontes rodriguezii or B. proboscidea occurring with Polysiphonia sp., Petalonia fascia and Ulva spp. developed in the late succession stage of the enriched sites. The very low time at which the community recovered in the enriched environments turned out to be one of the assets of the present study and this parameter can be used as a quick indicator of sewage pollution in the area.","palabras_clave":"BENTHIC ASSEMBLAGES |RECOVERY TIME|MARINE POLLUTION|BERKELEYA SP.|BOCCARDIA PROBOSCIDEA|BRACHIDONTES RODRIGUEZII"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6733,"idioma_id":4,"titulo":"Title: 'Striking modification of hippocampal GluN1 and GluN2A NMDA receptor subunits during long-term memory early consolidation in rats'","resumen":"SummaryWe previously showed that hippocampal GluN1 and GluN2A NMDA receptor subunits significantly increased in 2 months old rats following habituation to a novel open field (OF), while GluN2B remained unchanged. Here we showed that 1, 2 and 3 months old rats trained 5min in the OF, showed higher hippocampal GluN1 and GluN2A levels 70-75 minutes later, than animals euthanized immediately after that session, indicating that the subunits rise is a general feature for young rats. In other structures as prefrontal cortex (PFC) and amygdala, no significant changes were found. GluN1 and GluN2A increased in the hippocampus though not in PFC, 70 minutes after new object recognition (NOR) training session; and there were not changes in both structures after test session, 24h later. Although the raise is relatively long-lasting (1½ to 4h), it is transient, strengthened the idea of a putative physiological role. Hippocampal changes overlapped with memory early consolidation period suggesting a possible relationship between them. However, those rats had explored the same OF in 3 consecutive days and the only difference in the 4th day was the presence of two similar objects. Hence, we suggest that an enough relevant change in the environment would elicit significant changes in ?recognition\/ habituation representation?, which would involve subunits modification. In mature primary cultures of hippocampal neurons there was an increase in GluN1 and GluN2A following chemical plasticity induction, reaching a maximum at 75 minutes and returning to control levels 90 minutes later, in bodies and dendrites, showing a close related regulation. GluN2A increase depends on both local translation and preexisting transported GluN2A-NMDAR in similar proportions. These results support the idea that NMDAR subunits change would be related to spatial learning\/memory and hence, at that time interval only happens in the hippocampus where an environment representation\/map is being formed, leading to a LTM. Our in vivo and in vitro results suggest that: 1) the observed hippocampal subunits raise appears structure-specific, at least within that timing; 2) this phenomena is preserved along ages in young rats; 3) it could be related to early consolidation of new spatial information leading to LTM.","palabras_clave":"EDGE INTERSECTION GRAPHS|PATHS ON A GRID|FORBIDDEN INDUCED SUBGRAPHS|(NORMAL, HELLY) CIRCULAR ARC GRAPHS|POWERS OF CYCLES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6734,"idioma_id":4,"titulo":"Evaluation of stage-dependent genotoxic effect of Roundup® (glyphosate) on Caiman latirostris embryos","resumen":"The agricultural expansion over the last decades, along with the associated increase in the use of pesticides, represents a high risk for many wild species. Caiman latirostris is a South American caiman with many features that make it highly vulnerable to pesticide exposure. Considering previous finding on the genotoxicity of the glyphosate-based formulation Roundup® in this species, the aim of this study was to evaluate the possible stage-dependent effect of this compound on C. latirostris embryos through the Comet assay (CA), Micronuclei (MN) and Nuclear Abnormalities (NA) tests. Caiman eggs were exposed to three effective concentrations of Roundup® (750, 1250, 1750 µg\/egg) in three different stages of the incubation period (total duration 70 ± 3 days at 31 ± 2 °C) of approximately 23 days each. A statistically significant difference in DNA damage determined by the CA was found between groups exposed to different concentrations of RU (p0.05). There was no differences in the MN or NA frequencies between the different groups and the negative control (p>0.05), nor among the different stages within each treatment. The results obtained in this study indicate that RU produce DNA damage on C. latirostris embryos independently of the developmental stage where the exposure occurs, implying an important risk for the species during all its period of development, when pesticide application is at maximum rate.","palabras_clave":"REPTILES|EMBRYOTOXICITY|PESTICIDES|MICRONUCLEUS TEST|COMET ASSAY|NUCLEAR ABNORMALITIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6735,"idioma_id":4,"titulo":"Immunotoxicity of commercial-mixed glyphosate in broad snouted caiman (Caiman latirostris)","resumen":"The expansion and intensification of agriculture during thepast 50 years is unprecedented, and thus environmental problems have beentriggered at different scales. These transformations have caused the lossof habitat and biodiversity, and disruption of the structure andfunctioning of ecosystems. As a result of the expansion of theagricultural frontier in the recent past, many areas of the naturalgeographic distribution of the local wildlife, among them crocodiliansand particularly the broad snouted caiman (Caiman latirostris), have beenexposed to contaminants in all stages of life.The present study was designed to evaluate the effect of commerciallymixedglyphosate (RU) on some parameters of the immune system of C.latirostris. Two groups of caimans were exposed for two months todifferent concentrations of RU recommended for its application in thefield, while one group was maintained as an unexposed control. The RUconcentration was progressively decreased through the exposure period tosimulate glyphosate degradation in water. After exposure, total anddifferential white blood cell (WBC), and complement system activity (CS)were determined. In addition, the animals were injected with a solutionof lipopolysaccharide (LPS) from Escherichia coli to trigger an immuneresponse and evaluate the parameters associated with it.The results showed that an effect of the herbicide on CS was observed, asanimals exposed to RU showed a lower CS activity than animals from thenegative control (NC) but not in WBC. In the case of leukocyte populationcounts, differences were only found for heterophils and lymphocytes.","palabras_clave":"BROAD-SNOUTED CAIMAN|COMPLEMENT SYSTEM|WHITE BLOOD CELLS|GLYPHOSATE|IMMUNOTOXICITY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6736,"idioma_id":4,"titulo":"The structure of the bulk and the (001) surface of V2O5. A DFT+U study","resumen":"GGA (PW91) + U is applied to the calculation of the structure (lattice parameters) and the electronic structure of the V2O5 bulk and its (001) surface for different values of Ueff used in the literature (0.0, 3.0 and 6.6 eV). Similar surface lattice parameters are calculated for the (001) surface and for the bulk, as well as similar electronic structures. The calculated lattice parameters (a and b for the surface and a, b and c for the bulk) are in good agreement with experimental results. It seems that there is no strong correlation between the calculated lattice parameters and the value of Ueff. The calculated width of the valence band keeps the value of »5 eV for the three studied Ueff. However, the energy gap between the valence and the conduction bands increases with the value of Ueff. Ueff=3.0 eV seems to be the most adequate value to describe the energy gap after comparison with experimental results. Electronic density contour plots indicate that for a larger (smaller) Ueff the accumulated charge in the V-O(1) bond is overestimated (underestimated). The contour plots (in the a direction) show that the charge distribution V-O(3) is less correlated with Ueff than the charge distribution V-O(1), whereas charge distribution V-O(2) seems not to be corretaled with Ueff. The energy gap between the valence and theconduction bands seems to be strongly related with the charge&nbsp; distribution in the V-O(1) bond. The V-O(1) bond stability seems to be correlated with Ueff. However, the stability of the V-O(2) and V-O(3) bonds seems not to be strongly affected by Ueff.","palabras_clave":"DIVANADIUM PENTOXIDE SURFACE|ELECTRONIC STRUCTURE|DENSITY FUNCTIONAL THEORY|LATTICE CONSTANTS|ENERGY GAP"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6737,"idioma_id":4,"titulo":"Altered gene expression in hippocampus and depressive-like behavior in young adult female mice by early protein malnutrition","resumen":"Perinatal development represents a critical period in the life of an individual. A common cause of poor development is that which comes from undernutrition or malnutrition. In particular, protein deprivation during development has been shown to have deep deleterious effects on brain´s growth and plasticity. Early-life stress has also been linked with an increased risk to develop different psychopathologies later in life. We have previously shown that perinatal protein malnutrition in mice leads to the appearance of anxiety-related behaviors in the adulthood. We also found evidence that the female offspring was more susceptible to the development of depression-related behaviors. In the present work, we further investigated this behavior together with its molecular bases. We focused our study on the hippocampus, as it is a structure involved in coping with stressful situations. We found an increase in immobility time in the forced swimming test in perinatally malnourished females, and an alteration in the expression of genes related with neuroplasticity, early growth response 1, calcineurin and c-fos. We also found that perinatal malnutrition causes a reduction in the number of neurons in the hippocampus. This reduction, together with altered gene expression, could be related to the increment in immobility time observed in the forced swimming test.","palabras_clave":"malnutrition|depressive|hippocampus|gene expression"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6738,"idioma_id":4,"titulo":"Molecular detection of Thecaphora frezii in peanut (Arachis hypogaea L.) seeds","resumen":"Thecaphora frezii is the causal agent of peanut smut, important disease in the Argentine production area. The pathogen can be dispersed by kernels, through surface contamination or small lesions. The aim of this work was to design specific primers to develop a Polymerase Chain Reaction assay for the detection of Thecaphora frezii in peanuts kernels. The ITS region of four T. frezii isolates was amplified and sequenced. A consensus sequence was obtained and a pair of primers specific for the pathogen, named TF-2F and TF-2R, was synthesized. The primers were used for specificity and sensitivity tests. The former test involved a PCR using DNA from T. frezii and other seed-transmitted pathogens (S. rolfsi, S. minor, Fusarium solani and S. sclerotiorum). A 190 bp fragment was obtained for the T. frezii isolates, whereas no amplification was observed for the remaining pathogens. For the sensitivity test, positive results were observed at concentrations up to 3 × 10&#8722;4 pg of the target DNA. The specificity of this method, along with its sensitivity, makes it an important tool for epidemiological studies of peanut smut.","palabras_clave":"Thecaphora |Peanut|smut|Arachis"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6739,"idioma_id":4,"titulo":"Searching for molecular insight on hybridisation in Nothofagus spp. forests at Lagunas de Epulauquen, Argentina","resumen":"Lagunas de Epulauquen forests (36º49?S; 71º04?W) have been considered the northernmost population of Nothofagus obliqua in Argentina. Recently, however, its taxonomic status has been questioned due to its morphological, architectural and genetic distinctiveness. The convergence of migratory routes and hybridization with Nothofagus alpina are the two main hypotheses proposed to explain the distinctiveness of this population. Despite its unique characteristics, no evaluation of its putative origin has been carried out. In order to test the hypothesis of hybridization we analyzed nuclear Adh and ITS fragment sequences together with 12 nuclear microsatellites. These nuclear regions were selected based on their previously reported divergence between N. obliqua and N. alpina. The survey was conducted in 12 individuals from Lagunas de Epulauquen, and individuals from N. obliqua (8), N. alpina (1), N. macrocarpa (2), N. glauca (4) and N. leonii (2). By combining a phylogenetic analysis with a principal coordinate analysis of microsatellite data, we showed that Lagunas de Epulauquen individuals were closely related to other N. obliqua individuals, while hybridization with N. alpina was not detected. Our results indicate that hybridization processes could be discarded as the origin of the Lagunas de Epulauquen distinctiveness. The hypothesis of the convergence of two N. obliqua migratory routes at this latitude was reinforced based on detected patterns of genetic diversity. Notwithstanding, its high geographical isolation seems to be also a key evolutionary factor to explain the distinctiveness of this population. Lagunas de Epulauquen population could be therefore included within Argentinean N. obliqua domestication and conservation programs.","palabras_clave":"MOLECULAR SYSTEMATICS|SOUTHERN BEECHES|PATAGONIA|SSR MARKERS|HYBRIDIZATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6740,"idioma_id":4,"titulo":"Learning an adjective. The impact of descriptive information \/ Aprendiendo un adjetivo. El impacto de la información descriptiva","resumen":"Abstract: Picture books are a symbolic means by which children often learnwords in everyday interactions with adults. The aim of this research was tostudy the impact of descriptive information on learning an adjective by usinga picture book. We found that whilst this information helped three-year-oldslearn the adjective, it was not effective for two-and-a-half-year-olds. It seemsthat for three-year-olds this descriptive clue contributed to the creation of azone of proximal development where the teaching\/learning process couldunfold. This research is in line with studies that underscore the central roleof social interaction in general and instruction in particular during the processof cognitive development and learning.Keywords: picture book; learning; adjective; descriptive informationResumen: Los libros ilustrados constituyen medios simbólicos a través de loscuales los niños suelen aprender palabras en interacciones cotidianas con losadultos. El objetivo de este trabajo fue estudiar el impacto de la informacióndescriptiva en el aprendizaje de un adjetivo mediante un libro ilustrado.Encontramos que esta información ayudó a los niños de tres años de edad aaprender el adjetivo, pero no fue efectiva a los dos años y medio. Parecería serque a los tres años esta pista descriptiva contribuyó a crear una zona dedesarrollo potencial donde desplegar el proceso de enseñanza\/aprendizaje.Este trabajo se alinea con investigaciones que destacan el rol central de lainteracción social en general y la instrucción en particular en el desarrollocognitivo y el aprendizaje.Palabras clave: libro de imágenes; aprendizaje; adjetivo; informacióndescriptiva","palabras_clave":"PICTUREBOOK|LEARNING|ADJECTIVE|DESCRIPTIVE INFORMATION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6741,"idioma_id":4,"titulo":"Optimal control of many-body quantum dynamics: Chaos and complexity","resumen":"Achieving full control of the time-evolution of a many-body quantum system is currently a major goal in physics. In this work we investigate the different ways in which the controllability of a quantum system can be influenced by its complexity, or even its chaotic properties. By using optimal control theory, we are able to derive the control fields necessary to drive various physical processes in a spin chain. Then, we study the spectral properties of such fields and how they relate to different aspects of the system complexity. We find that the spectral bandwidth of the fields is, quite generally, independent of the system dimension. Conversely, the spectral complexity of such fields does increase with the number of particles. Nevertheless, we find that the regular or chaotic nature of the system does not affect significantly its controllability.","palabras_clave":"Control Cuántico|Complejidad|Caos"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6742,"idioma_id":4,"titulo":"Exocrine-Endocrine Pancreas. Menopause and Estradiol Treatment. Analysis by the ?Pancreatogram?","resumen":"The aim of the present undertaken was to analyze, by means of the ?pancreatogram?, the changes induced by estradiol in a group of non-obese healthy women (n=15) in the menopause stage (mean age 57) in comparison with a control group (n=18) of all ages (mean 46). This exam allows an encompassing view of both the exocrine and endocrine pancreas and of their interactions.The main evaluation was done with the 2 hs cumulative values of glycemia, insulin, amylase, isoamylase, lipase and calcium. The menopause group was reevaluated after one month of estradiol treatment (estrogen stage) at the dose of 0,625 mg\/day. Unexpectedly 75% of the menopause women revealed an impaired glucose tolerance test, this reflected by the glycemia\/insulin index figures. The estrogen stage and the one performed following a free hormonal treatment period (post-treatment stage), disclosed a progressive significant decline of the glycemia values associated to unmodified insulin ones. This finding reflects an enhanced sensitivity response to the endocrine hormone. Coupled to the latter are associated the isolated respective values on the lipasemia fall and of calcemia rising. Coherent with these results are those observed with the index figures of lipase\/insulin and lipase\/calcium. Undoubtedly estradiol enhances both insulin and calcium secretion. Suggestively, these effects were more marked following a short arrest period of estradiol administration. This fact seemingly allowing to infer that in certain circumstances it would be advisable to choose an intermittent therapeutic option.","palabras_clave":"hepatic insulin sensitizing substance |Estradiol Stage|pancreatogram|menopause"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6743,"idioma_id":4,"titulo":"Prevalence and infection intensity of Rickettsia massiliae in Rhipicephalus sanguineus sensu lato ticks from Mendoza, Argentina","resumen":"Rickettsia massiliae belongs to the spotted fever group and in the New World is commonly associated with the brown dog tick, Rhipicephalus sanguineus. Herein we investigate the presence of R. massiliae in Rh. sanguineus sensu lato ticks in a location near the Andean foothills (Mendoza, Argentina), to provide a prevalence estimate and to assess the infection intensity of this pathogen. Rickettsia massiliae infection was found in 5.1% of the Rh. sanguineus s.l. ticks analyzed and only high infection intensities were observed. Molecular analysis determined that all R. massiliae-infected Rh. sanguineus s.l. ticks belonged to the temperate lineage.","palabras_clave":"RICKETTSIA|RHIPICEPHALUS SANGUINEUS|TEMPERATE LINEAGE|VECTORIAL COMPETENCE|INFECTION INTENSITY|ARGENTINA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6744,"idioma_id":4,"titulo":"New molecular data on subcutaneous Philornis (Diptera: Muscidae) from southern 4 South America suggests the existence of a species complex","resumen":"The taxonomic identification of species in the genus Philornis Meinert 24 (Diptera: Muscidae) has been based primarily upon analysis of morphological 25 characters. However, relying solely on morphology has led to frequent species 26 misidentification. This has been empirically demonstrated when specimens identified 27 morphologically as P. seguyi have been reclassified as P. torquans based on DNA 28 sequences of the second ribosomal internal transcribed spacer (ITS2), showing that the 29 morphologic distinction between these 2 species is extremely challenging. Herein, new 30 molecular data on specimens assigned to P. torquans and P. seguyi from the type 31 locality of P. seguyi (Misiones province, Argentina) were analyzed. The ITS2 sequences 32 obtained differed from those previously reported for Philornis, increasing to 3 the 33 number of documented genotypes of flies morphologically indistinguishable from P. 34 torquans. These results strongly suggest that subcutaneous Philornis spp. from southern 35 South America could represent a species complex (P. torquans complex).","palabras_clave":"PHILORNIS SEGUYI|PHILORNIS TORQUANS|ITS2|SPECIES COMPLEX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6745,"idioma_id":4,"titulo":"*- Hyaluronan oligomers sensitize chronic myeloid leukemic cell line to the effect of Imatinib","resumen":"Chronic myeloid leukemia is a myeloproliferative syndrome characterized by the presence of thePhiladelphia chromosome (Ph), generated by a reciprocal translocation occurring between chromosomes9 and 22 [t(9;22)(q34;q11)]. As a consequence, a fusion gene (bcr-abl) encoding a constitutivelyactive kinase is generated. The first-line treatment consists on BCR-ABL inhibitors such as Imatinib,Nilotinib and Dasatinib. Nevertheless, such treatment may lead to the selection of resistant cells.Therefore, finding molecules that enhance the anti-proliferative effect of first-line drugs is of value.Hyaluronan oligomers (oHA) are known to be able to sensitize several tumor cells to chemotherapy.We have previously demonstrated that oHA can revert Vincristine resistance in mouse lymphomaand human leukemia cell lines. However, little is known about the role of oHA in hematologicalmalignancies. The aim of this work was to determine whether oHA are able to modulate the antiproliferativeeffect of Imatinib in chronic myeloid leukemia (CML) cell lines. The effect on apoptosisand senescence as well as the involvement of signaling pathways were also evaluated. For thispurpose, the human CML cell lines K562 and Kv562 (resistant) were used. We demonstrated thatoHA sensitized both cell lines to the anti-proliferative effect of Imatinib increasing apoptosis andsenescence. Moreover, this effect would be accomplished through the down-regulation of thePI3K signaling pathway. These findings highlight the potential of oHA when used as a co-adjuvanttherapy for chronic myeloid leukemia.","palabras_clave":"LMC|Acido hialurònico|Oligosacaridos|Imatinib"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6746,"idioma_id":4,"titulo":"Chronic Pregabalin Treatment Decreases Excitability of Dentate Gyrus and Accelerates Maturation of Adult-born Granule Cells","resumen":"Pregabalin (PGB) is extensively prescribed to treat neurological andneuropsychiatrical conditions such as neuropathic pain, anxiety disorders and epilepsy.Although PGB is known to bind selectively to the &#945;2&#948; subunit of voltage gated calciumchannels, there is little understanding about how it exerts its therapeutic effects. In thisarticle we analyzed the effects of an in vivo chronic treatment with PGB over thephysiology of dentate gyrus granule cells (DGGCs) using ex vivo electrophysiological andmorphological analysis in adult mice. We found that PGB decreases neuronal excitabilityof DGGCs. In addition, PGB accelerates maturation of adult-born DGGCs, an effect thatwould modify dentate gyrus plasticity. Together, these findings suggest that PGB reducesactivity in the dentate gyrus and modulates overall network plasticity, which mightcontribute to its therapeutic effects","palabras_clave":"Dentate Gyrus, |Neurogenesis, |Pregabalin,| Gabapentin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6747,"idioma_id":4,"titulo":"THE Rab3A-22A CHIMAERA PREVENTS SPERM EXOCYTOSIS BY STABILIZING OPEN FUSION PORES","resumen":"At the final stage of exocytotis, a fusion pore opens between the plasma and a secretory vesicle membranes; typically, when the pore dilates the vesicle releases its cargo. Sperm contain a large dense-core secretory granule (the acrosome) whose contents are secreted by regulated exocytosis at fertilization. Minutes after the arrival of the triggering signal, the acrosomal and plasma membranes dock at multiple sites and fusion pores open at the contact points. It is believed that immediately afterwards, fusion pores dilate spontaneously. Rab3A is an essential component of human sperm?s exocytotic machinery. Yet, recombinant, persistently active Rab3A halts calcium-triggered secretion when introduced after docking into streptolysin O-permeabilized cells; so does a Rab3A-22A chimaera. Here, we applied funcional assays, electron and confocal microscopy to show that the secretion blockage is due to the stabilization of open fusion pores. Other novel findings are that sperm SNAREs engage in &#945;-SNAP\/NSF-sensitive complexes at a post-fusion stage. Complexes are disentangled by these chaperons to achieve vesiculation and acrosomal contents release. Thus, post-fusion regulation of the pores determines their expansion and the success of the acrosome reaction.","palabras_clave":"Rab3-22|EXOCYTOSIS|HUMAN SPERM|FUSION PORE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6748,"idioma_id":4,"titulo":"Development of strong enzymatic biocatalysts for dye decolorization","resumen":"Escherichia coli crude laccase was used to develop an immobilized biocatalyst with improved stability.Chelating and entrapment immobilization techniques were studied,and laccase encapsulation in Cu-alginate gels showed the best results.This biocatalyst was active at different conditions of pH,tem-perature and ionic strength,and was able todecolorize the carcinogenicdyes Trypan Blue, Bromothymol Blue and Coomassie Brilliant blue R with yields close to 90% without mediator addition. The Cu-alginate derivatives retained more than 70% of catalytic activity for at least 430h of continuous use.The results demonstrated that immobilized laccase has potential application sindyestuff treatment.","palabras_clave":"Laccase|Immobilization|TrypanBlue|Escherichiacoli"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6749,"idioma_id":4,"titulo":"New developments in nucleoside analogues biosynthesis: A review","resumen":"The present review deals with current advances in the chemoenzymatic synthesis of biologically important nucleoside analogues (NA), either by the use of microorganisms or enzymes as biocatalysts. The interest in exploiting these biocatalysts is constantly increasing nowadays because of the advantages they have with respect to classic organic chemistry synthesis, such as a fewer number of synthesis steps, an improved chemo-, regio- and stereoselectivity, high catalytic efficiency, and simple subsequent processing. Besides, this technology offers an environmentally friendly alternative in comparison with synthetic chemistry. Thus, the present article gives a brief outline of emerging methodologies for the biosynthesis of NAs commonly used in cancer therapies, such as cladribine, clofarabine, nelarabine, fludarabine, decitabine, cytarabine and floxuridine, and as antivirals: ribavirin, iduviran, vidarabine, acyclovir, lamivudine and emtricitabine from microbial or enzymatic sources, and their potential applications in the biotechnology industry. Also, it points highlights the importance of subsequent modifications of nucleoside analogues by different enzymes used as biocatalysts in order to improve the pharmacological properties of the existing drugs. Moreover, the importance of biocatalyst immobilization for industrial applications is considered.","palabras_clave":"Green chemistry|Immobilization|Antitumor agent|Antiviral compound"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6750,"idioma_id":4,"titulo":"Conformationalensembleof\thumana-synuclein\t physiological form predicted \tby molecular \t simulations","resumen":"WeperformhereenhancedsamplingsimulationsofN-terminallyintervention in PD. acetylatedhumana-synuclein,anintrinsicallydisorderedproteinunknown, as are the mechanism(s) underlying the structural transi- involvedinParkinson?sdisease.Thecalculations,consistentwithtion from the innocuous, monomeric disordered conformations of experiments,suggestthatthepost-translationalmodificationleadsa-synucleintoitsneurotoxicforms.Recently,abundantevidencetotheformationofatransientamphipathica-helix.Thelatter,revealedthatthemonomericstateofa-synuclein undergoes 5?7 absentinthenon-physiologicalform,altersproteindynamicsatN-terminalacetylationinvivo(AcAS,hereafter),whichdifferstheN-terminalandintramolecularinteractions.structurallyinasmallbutsubtlewayfromthewidelystudied,","palabras_clave":"synuclein|molecular dynamics|native structure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6751,"idioma_id":5,"titulo":"La extensión en la formación de grado del Licenciado en Turismo.  El proyecto 'Concientización turística ambiental en destinos costeros'","resumen":"Se presentan los resultados de las actividades llevadas a cabo en el proyectode extensión ?Concientización turística ambiental en destinos costeros?, de laUniversidad Nacional de Mar del Plata, con el objetivo de analizar la contribuciónde la extensión a la investigación y formación de grado del Licenciado enTurismo. Se articuló con la asignatura Práctica Profesional Comunitaria, en laque los estudiantes realizaron actividades de concientización en problemáticasambientales dirigidas a diferentes actores y consensuaron acciones para mejorarla contribución del turismo al desarrollo local en Santa Clara del Mar (Argentina).Se aplicó la metodología de Investigación Acción Participativa (IAP), lo cualpermitió que el accionar del grupo contribuya con la formación del Licenciadoen Turismo mediante la capacitación en temas turísticos y ambientales, en tantoque su actuación generó una mayor conciencia en los actores locales sobreel adecuado manejo de los espacios turísticos y costeros, con el consecuentefavorecimiento del desarrollo local.","palabras_clave":"EXTENSIÓN|INVESTIGACIÓN|ACCIÓN PARTICIPATIVA|ACTORES SOCIALES|TURISMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6752,"idioma_id":4,"titulo":"Spectroscopic\tandTheoreticalStudyofCu\tBinding\tto\tHis111\tin\tthe\t HumanPrion\tProteinFragment106&#8722;115","resumen":"The ability of the cellular prion protein (PrPC) to bind copper in vivo points to a physiological role for PrPC in copper transport. Six copper binding sites have been identified in the nonstructured N-terminal region of human PrPC. Among these sites, the His111 site is unique in that it contains a MKHM motif that would confer interesting CuI and CuII binding properties. We have evaluated CuI coordination to the PrP(106?115) fragment of the human PrP protein, using NMR and X-ray absorption spectroscopies and electronic structure calculations. We find that Met109 and Met112 play an important role in anchoring this metal ion. CuI coordination to His111 is pH-dependent: at pH >8, 2N1O1S species are formed with one Met ligand; in the range of pH 5?8, both methionine (Met) residues bind to CuI, forming a 1N1O2S species, where N is from His111 and O is from a backbone carbonyl or a water molecule; at pH <5, only the two Met residues remain coordinated. Thus, even upon drastic changes in the chemical environment, such as those occurring during endocytosis of PrPC (decreased pH and a reducing potential), the two Met residues in the MKHM motif enable PrPC to maintain the bound CuI ions, consistent with a copper transport function for this protein. We also find that the physiologically relevant CuI-1N1O2S species activates dioxygen via an inner-sphere mechanism, likely involving the formation of a copper(II) superoxide complex. In this process, the Met residues are partially oxidized to sulfoxide; this ability to scavenge superoxide may play a role in the proposed antioxidant properties of PrPC. This study provides further insight into the CuI coordination properties of His111 in human PrPC and the molecular mechanism of oxygen activation by this site.","palabras_clave":"Cu(I)|prion protein|biophysics"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6753,"idioma_id":5,"titulo":"Caracterizacion de la unión cemento esmalte de dientes permanentes del perro. Resultados preliminares","resumen":"Introducción: La unión cemento-esmalte (UCE) es el sitio del diente donde se relacionan el esmalte, que cubre a la corona, y el cemento, que cubre a la raíz. La forma en que interaccionan el esmalte y el cemento es variable. Esta relación fue estudiada inicialmente por Choquet, en 1899, a través del uso del microscopio óptico (MOC) [1].  Observó que, en el hombre, existían diferentes  formas de interrelación de los tejidos que involucran a la UCE, describiendo cuatro tipos a los que se denominó ?casos de Choquet?. En el ?caso 1? el cemento termina superponiéndose y cubriendo al esmalte (fig. 1); en el ?caso 2? el esmalte termina cubriendo al cemento, en el ?caso 3? el cemento y esmalte terminan en un mismo punto sin superponerse (fig. 2), y por último, en el ?caso 4? el cemento termina antes de contactar con el esmalte, quedando una pequeña franja de dentina expuesta (fig. 3). En el año 2000, Neuvald y Consolaro llevaron a cabo el primer  estudio completo de la morfología de la UCE en los distintos tipos de dientes permanentes del humano [2], en el que se describió y analizó la interrelación de los tejidos involucrados por medio de la microscopía electrónica de barrido (MEB).  En el caso del perro, se han  identificado los cuatro casos de Choquet por medio del MEB. Sin embargo, nada se sabe sobre su presentación según el sitio de la UCE, por lo que el objetivo de esta comunicación es caracterizar la forma de presentación de los diferentes casos de Choquet en relación con el sector de la UCE (vestibular y linguo-palatino) de dientes permanentes de perro utilizando el MEB. Materiales y método: El estudio se realizó sobre 40 dientes permanentes de perro de diferente tipo (10 incisivos, 4 caninos, 14 premolares, 4 muelas carniceras superiores e inferiores y 8 molares), provenientes  de individuos jóvenes (de seis meses a dos años) muertos por distintas patologías (extraorales), o sacrificados por razones humanitarias. Se constató que las piezas dentales estuvieran sanas, sin ningún tipo de lesiones ni evidencia de enfermedad periodontal Los dientes se extrajeron con cuidado de no dañar la UCE, se conservaron en formol al 10%, se lavaron con agua corriente y se sumergieron en una solución de hipoclorito de sodio al 2,5% durante una semana para eliminar los tejidos blandos adheridos. Se lavaron nuevamente con agua corriente y se seccionaron en sentido mesio-distal con micromotor y disco de diamante. Se realizó un nuevo lavado de las piezas dentales con un sistema de circulación continuo de agua corriente durante una hora. Luego, las muestras se  deshidrataron utilizando concentraciones crecientes de alcohol, se secaron al aire y se montaron en platinas con la superficie seccionada en contacto con la misma, identificando cada platina con tipo de diente y sector del mismo (vestibular, palatino, o lingual) generando 40 muestras de la cara lingual\/palatina y 40 del lado vestibular de los dientes. Por último, se metalizaron con oro-paladio para ser observados y fotografiados en el MEB. Resultados: Se pudieron analizar 36 muestras correspondientes a la cara lingual\/palatina y 38 de la cara vestibular de los dientes obtenidos. La observación al MEB mostró diferentes situaciones. Por un lado, se evidenciaron  uniones cemento-esmalte  homogéneas que correspondieron algunas al ?caso de Choquet? 3 y otras al  4. Por otro lado, también se evidenció la presencia de uniones cemento-esmalte heterogéneas, que presentaron combinaciones entre los casos 3 y 4,  y entre los casos 1 y el 3. En cuanto al sector de la UCE, en la cara lingual\/palatina la frecuencia relativa fue: ?caso 3? 36,11%, ?caso 4? 22,22%, combinación ?caso 1 y 3? 11,11%, combinación ?caso 3 y 4? 30,55% (gráfico 1). En la cara vestibular la frecuencia relativa encontrada fue: ?caso 3? 50%, ?caso 4? 15,78%, combinación ?caso 1 y 3? 5,26%, combinación ?caso 3 y 4? 28,94% (gráfico 2) . Conclusiones: A partir del análisis de las muestras observadas, se concluye que la relación de los tejidos dentales varía a lo largo de la UCE, siendo en algunos casos más uniforme y en otros más heterogénea. Basados en las observaciones realizadas, el caso de Choquet más frecuente fue el 3, presentándose con mayor frecuencia del lado vestibular.  La combinación de Caso 3 y 4 fue la segunda más frecuente, sin presentar diferencias significativas entre la cara lingual\/palatina y la vestibular. El caso 4 resultó ser el tercero más observado, siendo más frecuente del lado lingual\/palatino de las muestras. Y por último, la combinación de caso 1 y 3 fue la menos frecuente, siendo más observada en la cara lingual\/palatina de los dientes procesados. No se encontraron muestras con la presencia del caso 1 de manera homogénea, ni muestras que presenten el caso 2. Hace falta continuar con el estudio de un mayor número de muestras con el fin de lograr obtener  resultados más concluyentes. Este trabajo forma parte del Proyecto UBACyT","palabras_clave":"esmalte|cemento|union|perro"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6754,"idioma_id":4,"titulo":"Draft Genome Sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a Citrate-Fermenting Strain","resumen":"We report the draft genome sequence of Lactococcus lactis subsp. lactis bv. diacetylactis CRL264, a natural strain isolated from artisanal cheese from northwest Argentina. L. lactis subsp. lactis bv. diacetylactis is one of the most important microorganisms used as starter culture around the world.The CRL264 strain constitutes a model microorganism in the studies on the generation of aroma compounds(diacetyl,acetoin,and 2,3butanediol) by lactic acid bacteria. Our genome analysis shows similar genetic organization to other available genomes of L. lactis bv. diacetylactis strains.","palabras_clave":"Bioinformatics|Genome sequencing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6755,"idioma_id":4,"titulo":"Draft Genome Sequences of Four Enterococcus faecium Strains Isolated from Argentine Cheese","resumen":"We report the draft genome sequences of four Enterococcus faecium strains isolated from Argentine regional cheeses. These strains were selected based on their technological properties,i.e.,their ability to produce aroma compounds(diacetyl,acetoin,and 2,3-butanediol)from citrate. The goal of our study is to provide further genetic evidence for the rational selection of enterococci strains based on their pheno- and genotype in order to be used in cheese production.","palabras_clave":"Bioinformatics|Genome Sequencing"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6756,"idioma_id":4,"titulo":"Functional analysis of the citrate activator CitO from Enterococcus faecalis implicates a divalent metal in ligand binding","resumen":"The regulator of citrate metabolism, CitO, from Enterococcus faecalis belongs to the FCD family within the GntR superfamily. In the presence of citrate, CitO binds to cis-acting sequences located upstream of the cit promoters inducing the expression of genes involved in citrate utilization. The quantification of the molecular binding affinities, performed by isothermal titration calorimetry (ITC), indicated that CitO has a high affinity for citrate (KD = 1.2 ± 0.2 &#956;M), while it did not recognize other metabolic intermediates. Based on a structural model of CitO where a putative small molecule and a metal binding site were identified, it was hypothesized that the metal ion is required for citrate binding. In agreement with this model, citrate binding to CitO sharply decreased when the protein was incubated with EDTA. This effect was reverted by the addition of Ni2+, and Zn2+ to a lesser extent. Structure-based site-directed mutagenesis was conducted and it was found that changes to alanine in residues Arg97 and His191 resulted in decreased binding affinities for citrate, as determined by EMSA and ITC. Further assays using lacZ fusions confirmed that these residues in CitO are involved in sensing citrate in vivo. These results indicate that the molecular modifications induced by a ligand and a metal binding in the C-terminal domain of CitO are required for optimal DNA binding activity, and consequently, transcriptional activation.","palabras_clave":"Enterococcus|metalloprotein|citrate|FCD domain"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6757,"idioma_id":5,"titulo":"Nanopartículas de goetita en Eisenia andrei: Ingreso, efectos subcelulares y su influencia en la acumulación de Cd y Pb","resumen":"El objetivo de este trabajo consistió en investigar la incorporación de nanopartículas (NPs) de goetita (&#945;FeOOH) en la lombriz terrestre Eisenia andrei a través de diferentes vías de exposición. En primer lugar, los organismos fueron expuestos a un suelo artificial adicionado con diferentes suspensiones de NPs para evaluar la absorción a través del tracto gastrointestinal. Después de 10 días, los animales fueron depurados y el contenido de Fe se analizó por espectroscopía de absorción atómica (AAS). Sorprendentemente, los animales expuestos a las NPs presentaron niveles de Fe menores que los controles. Además, no seobservó una correlación entre los niveles de NPs en las suspensiones y los valores de concentración de Fe en las lombrices. El ingreso por vía dérmica también fue investigado exponiendo a las lombrices en contacto con papeles de filtro que contenían una suspensión de NPs (50 &#956;g cm-2) durante diferentes tiempos de exposición aguda (24, 48, y 72 h). En los dos primeros períodos de exposición (24 y 48 h) los niveles de Fe aumentaron con respecto a los que se encontraron en los organismos  controles. Sin embargo, después de un período de exposición de 72 h, los niveles en las lombrices expuestas fueron menores que en los controles. De manera de explicar este inesperado comportamiento, se evaluó la capacidad antioxidante total en los tejidos de organismos expuestos a papeles de filtro por 72 h, usando el ensayo de TOSC. Se encontró un descenso significativo en la capacidad antioxidante total en los organismos expuestos. Finalmente, se investigó la influencia de las NPs de goetita en el ingreso de Cd y Pb. En este caso, los bioensayos se realizaron exponiendo a las lombrices a papeles de filtro que contenían una solución de cada metal en presencia o ausencia de una suspensión de NPs (50 &#956;g cm-2) por 72 h. Los valores de concentración de Cd y Pb seanalizaron por AAS. Tanto para Cd como para Pb se encontraron descensos significativos en la acumulación de ambos metales (aproximadamente 38%) en las lombrices expuestas a cada metal en presencia de la suspensión de NPs de goetita. En conclusión, la liberación de NPs en ecosistemas terrestres puede inducir algunas respuestas inesperadas en las lombrices y también las NPs pueden interactuar con otros contaminantes preexistentes en el ambiente, modificando su biodisponibilidad","palabras_clave":"EISENIA ANDREI|LOMBRICES TERRESTRES|NANO-GOETITA|NANOTOXICIDAD"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6758,"idioma_id":4,"titulo":"Nonlinear Stefan problem with convective boundary condition in Storm's materials","resumen":"We consider a nonlinear one-dimensional Stefan problem for a semi-innite materialx > 0; with phase change temperature Tf : We assume that the heat capacity and thethermal conductivity satisfy a Storm´s condition and we assume a convective boundary condition at the xed face x = 0. An unique explicit solution of similarity type is obtained. Moreover, asymptotic behavior of the solution when h tends to infinity is studied.","palabras_clave":"STEFAN PROBLEM|FREE BOUNDARY PROBLEM|PHASE_CHANGE PROCESS|SIMILARITY SOLUTION"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6759,"idioma_id":4,"titulo":"TECTONOSTRATIGRAPHIC HISTORY OF THE NEOGENE MAIMARA BASIN, NORTHWEST ARGENTINA.","resumen":"This paper presents the tectonostratigraphic evolution of the Maimara Basin and explores the relationship between the clastic sediments and pyroclastic deposits in the basin and the evolution of the adjacent orogeny and magmatic arc. The sedimentary facies in this part of the basin include, in ascending order, an ephemeral fluvial system, a deep braided fluvial system and a medial to distal ephemeral fluvial system. We interpret that Maimara Formation accumulated in a basin that has developed two stages of accumulation. Stage 1 extended from 7 to 6.4 Ma and included accelerated tectonic uplift in the source areas, and it corresponds to the ephemeral fluvial system deposits. Stage 2, which extended from 6.4 to 4.8 Ma, corresponds to a tectonically quiescent period and included the development of the deep braided fluvial system deposits. The contact between the Maimara and Tilcara formations is always characterized by a regional unconformity and, in the study area, also shows pronounced erosion.Rare earth element and other chemical characteristics of the tuff intervals in the Maimara Formation fall into two distinct groups suggesting the tuffs were erupted from two distinct late Miocene source regions. The first and most abundant group has characteristics that best match tuffs erupted from theGuacha, Pacana and Pastos Grandes calderas, which are located 200 and 230 km west of the study area at 22º-23º30´0 S latitude. The members the second group are chemically most similar to the Merihuaca Ignimbrite from the Cerro Galan caldera 290 km south-southwest of the studied section. The distinctive geochemical characteristics are excellent tools to reconstruct the stratigraphic evolution of the Neogene Maimara basin from 6.4 to 4.8 Ma.","palabras_clave":"Maimara formation |Paleomagnetism |Provenance |Geochemistry tuffs|Humahuaca"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6760,"idioma_id":5,"titulo":"Tensiones y desafíos en la construcción de un trabajo colaborativo entre docentes e investigadores en didáctica de la matemática","resumen":"En este artículo se comunican resultados que surgen de una investigación en la que se estudia la producción matemático didáctica que tiene lugar en el marco de un trabajo colaborativo entre docentes y directivos de escuela primaria e investigadores en didáctica de la matemática. Las reflexiones que se presentan se centran en dos cuestiones: a) los modos de problematizar el conocimiento matemático con los docentes y, b) la complejidad de la construcción de un proceso colaborativo. El análisis compartido con los maestros de la producción de sus alumnos contribuyó a la problematización del conocimiento a enseñar en tanto permitió identificar relaciones matemáticas que ampliaron aquello que se concebía posible en relación con los contenidos. La interpretación en términos de tensiones entre algunos aspectos constitutivos de la colaboración (capacitación-colaboración, naturalización-problematización y adaptación a condiciones institucionales-preservación de la colaboración) hace visible la complejidad de un proceso que solicita producir condiciones específicas y no se deja dominar por la sola voluntad de los participantes.","palabras_clave":"Didáctica Matemática|Formación docente|Trabajo colaborativo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6761,"idioma_id":4,"titulo":"Lactic acid bacteria decarboxylation reactions in cheese","resumen":"Fermentation in cheese comprises oxidation-reduction of carbohydrates to yield organic acids, alcohols and carbon dioxide. Furthermore, organic acid and amino acid metabolism produces a series of compounds that positively or negatively affect final cheese quality. Under the strong selective pressure of the acidic environment of cheese ripening, lactic acid bacteria have developed multiple stress-resistant strategies, including decarboxylase and deiminase reactions that play a main physiological role during the ripening process of cheese production. The control of the expression and activity of these enzymes is one active strategy for intracellular acid-base homeostasis. This review covers relevant pathways and aspects related to gene regulation of gene clusters present in starter or non-starter lactic acid bacteria that are involved in sensory changes such as flavour development. From the point of view of food safety the main decarboxylation pathways that lead to the formation of biogenic amines are described.","palabras_clave":"FOOD|CITRATE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6762,"idioma_id":4,"titulo":"Pelagic or benthic prey? Combining trophic analyses to infer the diet of a breeding South American seabird, the Red-legged Cormorant (Phalacrocorax gaimardi)","resumen":"The available information about the feeding habits and preferences of the Red-legged Cormorant(Phalacrocorax gaimardi) generally suggests that this near-threatened South American cormorant is a sedentary benthicforager that also incorporates pelagic prey in its diet. In order to describe the dietary composition and assess the importanceof certain prey types (pelagic vs benthic), we studied the diet of this cormorant on the Argentine Atlantic coast during fourbreeding seasons, using a combined technique of conventional diet assessment (pellets) and stable isotope analysis. Ourresults show that the Red-legged Cormorant forages mainly on pelagic and demersal?pelagic prey. Results of bothtechniques showed the main prey to be the Patagonian Sprat, a small high-energy-content pelagic forage fish, and thePatagonian Squid, a low-energy-content demersal?pelagic invertebrate. We also found an overall low prey diversity andimportant interannual variation for the main prey types, as well as variation between the different breeding stages. Thisstudy therefore contributes new and unambiguous information about the Red-legged Cormorant?s use of trophicresources and suggests that the combination of a conventional technique with stable isotope analysis provides a solidframework for this seabird diet assessment.","palabras_clave":"ARGENTINA|MIXING MODEL|PATAGONIA|SHAG|STABLE ISOTOPE ANALYSIS|TROPHIC ECOLOGY"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6763,"idioma_id":4,"titulo":"Highlights from the 1st Latin American meeting on metronomic chemotherapy and drug repositioning in oncology, 27-28 May, 2016, Rosario, Argentina","resumen":"Following previous metronomic meetings in Marseille (2011), Milano (2014), and Mumbai (2016), the first Latin American metronomic meeting was held in the School of Medical Sciences, National University of Rosario, Rosario, Argentina on 27 and 28 of May, 2016. For the first time, clinicians and researchers with experience in the field of metronomics, coming from different countries in Latin America, had the opportunity of presenting and discussing their work. The talks were organised in three main sessions related to experience in the pre-clinical, and clinical (paediatric and adult) areas. The different presentations demonstrated that the fields of metronomic chemotherapy and repurposing drugs in oncology, known as metronomics, constitute a branch of cancer therapy in permanent evolution, which have strong groups working in Latin America, both in the preclinical and the clinical settings including large, adequately designed randomised studies. It was shown that metronomics offers treatments, which, whether they are combined or not with the standard therapeutic approaches, are not only effective but also minimally toxic, with the consequent improvement of the patient´s quality of life, and inexpensive, a feature very important in low resource clinical settings. The potential use of metronomic chemotherapy was proposed as a cost\/effective treatment in low-\/middle-income countries, for adjuvant therapy in selected tumours. The fundamental role of the governmental agencies and non-governmental alliances, as the Metronomic Global Health Initiative, in supporting this research with public interest was underlined.","palabras_clave":"Breast cancer|Metastasis|Surgery|Desmopressin"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6764,"idioma_id":4,"titulo":"Improved stability in SBA-15 mesoporous materials as catalysts for photo-degradation processes","resumen":"SBA-15 materials were modified with Cr, Ni and Fe and characterized by ICP, SAXS, XRD, N2 adsorption,TPR and UVeVis DRS. Their photo-catalytic activity was evaluated for the degradation of Acid Orange 7(AO7) in aqueous suspensions irradiated by artificial UVeVis light. The results showed that the Crmodifiedcatalyst exhibited the highest activity. The presence of Cr6þ highly dispersed on the internalsurface of the SBA-15 structure would be responsible for this observed behavior. Then, when TiO2 wasloaded on this solid, the photo-activity was increased due to a heterojunction effect between the twometals. Moreover, the TiO2 cover resulted in the Cr species protection leading to a notable decrease of theCr leaching into the reaction medium. In addition, comparing with the already reported results formodified MCM-41 catalysts, the solid synthesized here allowed achieving the solution biodegradabilityin shorter irradiation periods and could be re-used even after four cycles without loss of activity. Thus,the high performance of this material leads to make it a very promising photo-catalyst for pre-treatmentof recalcitrant contaminants present in aqueous effluents.","palabras_clave":"NANOSTRUCTURES|STABILITY|METALLIC SPECIES|CATALYTIC PROPERTIES"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6765,"idioma_id":4,"titulo":"Enhanced Surface Interaction of Water Confined in Hierarchical Porous Polymers Induced by Hydrogen Bonding","resumen":"Hierarchical porous polymer systems are increasingly applied to catalysis, bioengineering or separation technology due to the versatility provided by the connection of mesopores with percolating macroporous structures. Nuclear magnetic resonance (NMR) is a suitable technique for the study of such systems as it can detect signals stemming from the confined liquid and translate this information into pore size, molecular mobility and liquid-surface interactions. We focus on the properties of water confined in macroporous polymers of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] with different amounts of cross-linker, in which a substantial variation of hydroxyl groups is achieved. As soft polymer scaffold may swell upon saturation with determined liquids, the use of NMR is particularly important as it measures the system in its operational state. This study combines different NMR techniques to obtain information on surface interactions of water with hydrophilic polymer chains. A transition from a surface induced relaxation in which relaxivity depends on the pore size to a regime where the organic pore surface strongly restricts water diffusion is observed. Surface affinities are defined through the molecular residence times near the network surface.","palabras_clave":"surface interaction|NMR|porous Materials"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6766,"idioma_id":4,"titulo":"Comprehensive Evaluation and Implementation of Improvement Actions in Butcher Shops","resumen":"Foodborne pathogens can cause acute and chronic diseases and produce a wide range ofsymptoms. Since the consumption of ground beef is a risk factor for infections with some bacterial pathogens, we performed a comprehensive evaluation of butcher shops, implemented improvement actions for both butcher shops and consumers, and verified the impact of those actions implemented. A comprehensive evaluation was made and risk was quantified on a 1?100 scale as high-risk (1?40), moderate-risk (41?70) or low-risk (71?100). A total of 172 raw ground beef and 672 environmental samples were collected from 86 butcher shops during the evaluation (2010?2011) and verification (2013) stages of the study. Ground beef samples were analyzed for mesophilic aerobic organisms, Escherichia coli and coagulase-positive Staphylococcus aureus enumeration. Salmonella spp., E. coli O157:H7, non-O157 Shiga toxin-producing E. coli (STEC), and Listeria monocytogenes were detected and isolated from all samples. Risk quantification resulted in 43 (50.0%) high-risk, 34 (39.5%) moderate-risk, and nine (10.5%) low-risk butcher shops. Training sessions for 498 handlers and 4,506 consumers were held. Re-evaluation by risk quantification and microbiological analyses resulted in 19 (22.1%) high-risk, 42 (48.8%) moderate-risk and 25 (29.1%) low-risk butcher shops. The count of indicator microorganisms decreased with respect to the 2010?2011 period. After the implementation of improvement actions, the presence of L. monocytogenes, E. coli O157:H7 and stx genes in ground beef decreased. Salmonella spp. was isolated from 10 (11.6%) ground beef samples, without detecting statistically significant differences between both study periods (evaluation and verification). The percentage of pathogens in environmental samples was reduced in the verification period (Salmonella spp., 1.5%; L. monocytogenes, 10.7%; E. coli O157:H7, 0.6%; nonO157 STEC, 6.8%). Risk quantification was useful to identify those relevant facts in butcher shops. The reduction of contamination in ground beef and the environment was possibleafter training handlers based on the problems identified in their own butcher shops. Our results confirm the feasibility of implementing a comprehensive risk management program in butcher shops, and the importance of information campaigns targeting consumers. Further collaborative efforts would be necessary to improve foodstuffs safety at retail level and at home.","palabras_clave":"EVALUATION|IMPLEMENTATION|ACTIONS|BUTCHER"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6767,"idioma_id":5,"titulo":"Evaluación diferencial de procesos mnésicos visuoespaciales en el Síndrome de Turner","resumen":"el síndrome de turner es un trastorno cromosómico que expresa unamorfología cerebral atípica afectando áreas corticales y circuitos deconexión funcionales asociados con dificultades en la organización ycodificación del material no verbal. el presente trabajo tuvo por objetivoanalizar las características de la memoria visuoespacial en mujeres condiagnóstico de síndrome de turner, con particular énfasis en los procesosde codificación, a los fines de aportar evidencias empíricas sobrelas características parti-culares de los procesos de aprendizaje de estapoblación clínica. Con un diseño ex post facto , retrospectivo, de dosgrupos, se aplicó el test de copia y de reproducción de memoria defiguras geométricas complejas de rey a 40 mujeres (20 grupo clínico y20 grupo control). Los resultados mostraron la existencia de diferenciassignificativas entre la muestra clínica y sus controles en el funcionamientomnésico visuoespacial, con desempeños significativamenteinferiores de las mujeres con diagnóstico de síndrome de turner en losprocesos de organización perceptiva y en la capacidad de recuerdoinmediato. estas evidencias permitirían sustentar la idea de que laexpresión génica diferencial característica del síndrome de turnerproduciría una organización cortical atípica, principalmente en elhemisferio derecho, generando dificultades en la integración intermodal yen el procesamiento de estímulos nuevos, lo que explicaría el déficit enlos procesos de codificación de datos visuoespaciales.","palabras_clave":"MORFOLOGÍA CEREBRAL|PROCESOS DE CODIFICACIÓN |APRENDIZAJE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6768,"idioma_id":4,"titulo":"Social networks and genetic algorithms to choose committees with independent members","resumen":"Choosing committees with independent members in social networks is essentially a group selection problem where independence, as the main selection criterion, can be measured by the social distance between group members. Although there are many solutions for group selection problems in social networks, such as target set selection or community detection, none of them have proposed an approach to select committee members based on independence as group performance measure. In this work, we propose a novel approach for independent node group selection in social networks. This approach denes an independence group function and a genetic algorithm in order to optimize it. We present a case study where we build a real social network with on-line available data extracted from a Research and Development (R&D) public agency, and then we compare selected groups with existing committees of the same agency. Results show that the proposed approach can generate committees that improve group independence compared with existing committees.","palabras_clave":"Committee|Group selection|Independence|Social network|Genetic algorithm"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6769,"idioma_id":4,"titulo":"Failure-recovery model with competition between failures in complex networks: a dynamical approach","resumen":"Real systems are usually composed by units or nodes whose activity canbe interrupted and restored intermittently due to complex interactionsnot only with the environment, but also with the samesystem. Majdand{z}i´c $et;al.$ [Nature Physics {f 10}, 34  (2014)] proposed a model to study systems in which active nodes failand recover spontaneously in a complex network and found that in thesteady state the density of active nodes can exhibit an abrupttransition and hysteresis depending on the values of theparameters. Here we investigate a model of recovery-failure from adynamical point of view. Using an effective degree approach we findthat the systems can exhibit a temporal sharp decrease in the fractionof active nodes. Moreover we show that, depending on the values of theparameters, the fraction of active nodes has an oscillatory regimewhich we explain as a competition between different failureprocesses. We also find that in the non-oscillatory regime, thecritical fraction of active nodes presents a discontinuous drop whichcan be related to a ``targeted´´ k-core percolation process. Finally, using mean field equations we analyze the space of parameters at which hysteresis and oscillatory regimes can be found.","palabras_clave":"Complex Networks|Dynamical systems"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6770,"idioma_id":4,"titulo":"Theoretical and Experimental Study of the Antioxidant Behaviors of 5-O-Caffeoylquinic, Quinic and Caffeic Acids Based on Electronic and Structural Properties","resumen":"The aim of this study was to elucidate the structural and electronic factors that determine the antioxidant capacity of 5-Ocaffeoylquinic, caffeic and quinic acids under different experimental conditions. Antioxidant capacity was measured usingdifferent in vitro assays, involving diverse mechanisms of antioxidant action, namely, radical scavenging or reduction. The mechanisms of these reactions were analyzed by a theoretical study using the Density Functional Theory. Results allow relating in vitro antioxidant capacity of these three compounds with their chemical structures. The antioxidant capacity experimentally observed for these three acids was interpreted considering the reaction mechanism involved, the nature andstability of the intermediate formed, the formation and reaction of secondary compounds, and the effect of the reaction medium. The main goal of this report is presenting a theoretical analysis of reactions implicated in the antioxidant capacity, identifying the causes that increase or decrease such property. Thus, we present a quantum mechanical description of the antioxidant properties involved with these three compounds, considering equilibria and secondary oxidations involved.","palabras_clave":"DFT|mechanism|antioxidant capacity|secondary oxidations|intermediates"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6771,"idioma_id":4,"titulo":"Solubility of Stevioside and Rebaudioside A in water, ethanol and their binary mixtures","resumen":"In order to investigate the solubility ofStevioside and Rebaudioside A in different solvents (ethanol, water,ethanol:water 30:70 and ethanol:water 70:30), supersaturated solutions of pre-crystalizedsteviol glycosides were maintained at different temperatures (from 5 °C to 50°C) to reach equilibrium. Under these conditions significant differences werefound in the extent of solubility. RebaudiosideA was poorly soluble in ethanol and water, and Stevioside was poorly soluble inwater. Solvent mixtures more effectively promoted solubilisation,and a significant effect of temperature on solubility was observed. The two steviol glycosides showedhigher solubilities and this behavior was promoted by the presence of the othersweetener. The polarity indices of the solvents were determined, and helped to explain theobserved behavior. Several solute-solvent and solute-solute interactionscan occur, along with the incidence of a strong affinity between solvents. Theobtained results are in accordance with technological applications of ethanol,water and their binary mixtures for Stevioside and Rebaudioside A separations.","palabras_clave":"Stevia|Stevioside|Rebaudioside"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6772,"idioma_id":4,"titulo":"Transgenic glyphosate-resistant oilseed rape (Brassica napus) as an invasive weed in Argentina: detection, characterization and control alternatives","resumen":"The presence of glyphosate-resistant oilseed rape populations in Argentina was detected and characterized. The resistant plants were found as weeds in RR soybeans and other fields. The immunological and molecular analysis showed that the accessions presented the GT73 transgenic event. The origin of this event was uncertain, as the cultivation of transgenic oilseed rape cultivars is prohibited in Argentina. This finding might suggest that glyphosate resistance could come from unauthorized transgenic oilseed rape crops cultivated in the country or as seed contaminants in imported oilseed rape cultivars or other seed imports. Experimentation showed that there are alternative herbicides for controlling resistant Brassica napus populations in various situations and crops. AHAS-inhibiting herbicides (imazethapyr, chlorimuron and diclosulam), glufosinate, 2,4-D, fluroxypyr and saflufenacil proved to be very effective in controlling these plants. Herbicides evaluated in this research were employed by farmers in one of the fields invaded with this biotype and monitoring of this field showed no evidence of its presence in the following years.","palabras_clave":"Brassica napus|Transgene escape|Glyphosate|GMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6773,"idioma_id":5,"titulo":"Eficiencia bancaria en Argentina. Comportamiento de los bancos entre 2005 y 2013","resumen":"El objetivo del trabajo es analizar la evolución de la eficiencia de ciertas entidades bancarias argentinas durante el período comprendido entre los años 2005 y 2013. En tal sentido, se buscará distinguir los factores que contribuyen a explicar tal desempeño. A tal efecto, se combinará el análisis envolvente de datos (DEA) con el índice de productividad de Malmquist (IPM). Esto permite medir las variaciones en la productividad a lo largo del tiempo y, al mismo tiempo, descomponer sus causas. Los resultados alcanzados muestran una mejora en la productividad total y en la eficiencia técnica para un grupo importante de las entidades analizadas.","palabras_clave":"EFICIENCIA BANCARIA|ANALISIS ENVOLVENTE DE DATOS|INDICE DE PRODUCTIVIDAD DE MALMQUIST|EFICIENCIA TECNICA"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6774,"idioma_id":4,"titulo":"Maize grain yield components and source-sink relationship as affected by the delay in sowing date","resumen":"Delaying maize (Zea mays L.) sowing date can diminish grain yields through reductions in the number, size and activity ofgrowing grains (sink strength) and\/or reductions in the assimilate supply (source capacity) to grains during the grain filling period. Whether the source capacity or the sink strength is the limiting factor for grain yield in late sown maize still remains unclear. Understanding source-sink relationships is relevant to optimize crop management practices, to identify critical processes for crop modelling and to develop breeding strategies. The objective ofthis work was to assess the effect ofdelays in maize sowing date on grain yield components and on the source-sink relationship during the grain filling period. Three well irrigated and fertilized maize field experiments were conducted at Balcarce, Argentina (37&#9702; 45 S, 58&#9702; 18 W; 130 m a.s.l.) during 2009?10; 2010?11 and 2011?12 cropping seasons. Sowing dates ranged from October to January covering a broad range of the seasonal photo-thermal variation. Grain yield was affected by sowing date and varied from 1680 g m&#8722;2 (early sowings) to 203 g m&#8722;2 (late sowings). Grain number per unit area was reduced proportionally less than weight per grain as sowing date was delayed. Variations in grain yield were related to the harvest index, and were closely associated with dry matter accumulation during the post-silking period. The variation of source capacity was higher than that of sink strength during the grain filling period and the source\/sink ratio decreased from early to late sowing dates. Results indicate that crop growth during the grain filling period was limited by the sink strength in early sowing dates and by the photosynthetic source capacity in the late ones.","palabras_clave":"Sowing date|Maize|Potential grain yield|Source-sink relationships|Radiation use efficiency"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6775,"idioma_id":4,"titulo":"Light  intensity independence during dynamic laser speckle analysis","resumen":"We explore some different normalizations of current dynamic laser speckle activity measures searching for their performance with respect t o the illumination inhomogeneity of the samples.Inertia Moment and AverageValue of Differences of theco-occurrence matrix ar e compared using a paint-drying case study on a uniform sample where attenuation in a portion of the illuminated areas introduced using a neutral density filter.In this way,all environmental conditions being equal but non-uniform illumination permits thecomparison on a better approximation to objectivity.The results presented show that it is possible to mitigate the effects of the illumination in theactivities measured by thedynamic laser speckle.","palabras_clave":"BIOSPECKLE|PAINT DRYING|CO OCCURRENCE MATRIX"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6776,"idioma_id":4,"titulo":"Characterization of spatial-temporal patterns in dynamic speckle sequences using principal component analysis","resumen":"Speckle is being used as a characterization tool for the analysis of the dynamics of slow-varyingphenomena occurring in biological and industrial samples at the surface or near-surface regions. The retrieved data take the form of a sequence of speckle images. These images contain information about the inner dynamics of the biological or physical process taking place in the sample. Principal component analysis (PCA) is able to split the original data set into a collection of classes. These classes are related to processes showing different dynamics. In addition, statistical descriptors of speckle images are used to retrieve information on the characteristics of the sample. These statistical descriptors can be calculated in almost real time and provide a fast monitoring of the sample. On the other hand, PCA requires a longer computation time, but the results containmore information related to spatial?temporal patterns associated to the process under analysis. This contribution merges both descriptions and uses PCA as a preprocessing tool to obtain a collection of filtered images, where statistical descriptors are evaluated on each of them. The method applies to slow-varying biological and industrial processes","palabras_clave":"PRINCIPAL COMPONENTS ANALYSIS|DYNAMIC SPECKLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6777,"idioma_id":4,"titulo":"Comparative brain morphology of Neotropical parrots (Aves, Psittaciformes) inferred from virtual 3D endocasts","resumen":"Psittaciformes is a very diversified group of non-passerine birds, with advanced cognitive abilities and highly developed locomotor and feeding behaviours. Using computed tomography and three dimensional (3D) visualization software, the endocasts of 14 extant Neotropical parrots were reconstructed, with the aim of analysing, comparing and exploring the morphology of the brain within the clade. A 3D geomorphometric analysis was performed, and the encephalization quotient was calculated. Also, brain morphological character states were traced onto a Psittaciformes tree in order to facilitate interpretation of morphological traits in a phylogenetic context. Our results indicate that: (1) there are two conspicuously distinct brain morphologies, one considered walnut type (quadrangular and wider than long) and the other rounded (narrower and rostrally tapered); (2) Psittaciformes possesses a noticeable notch between hemisphaeria that divides the bulbus olfactorius; (3) the plesiomorphic and most frequently observed characteristics of Neotropical parrots are rostrally tapered telencephalon in dorsal view, distinctly enlarged dorsal expansion of the eminentia sagittalis and conspicuous fissura mediana; (4) there is a positive correlation between body mass and brain volume; (5) psittacids are characterized by high EQ values that suggest high brain volumes than relative to their body masses; and (6) the endocranial morphology of the Psittaciformes as a whole is distinctive relative to other birds. This new knowledge on brain morphology offers much potential for further insight in paleoneurological, phylogenetic and evolutionary studies.    ","palabras_clave":"3-D brain reconstructions|Aves|endocranial morphology|geometric morphometrics|characters mapping"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6778,"idioma_id":4,"titulo":"Suggestions and Recommendations for Furure Research in Phytoremediation on Aquatic Environment","resumen":"Phytoremediation is an attractive and ecofriendly solution for remediationof polluted soils and water. Despite the large number of papers published onthis topic, discussion of the results in literature is valuable but insufficient inorder to take concrete and practical decisions in remediation processes byusing living plants. In this sense, there is a remarkable difference betweenapproaches used for living and nonliving biomass.","palabras_clave":"Biosorption|Bioccumulation|Kinectics|Equilibrium"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6779,"idioma_id":4,"titulo":"Conditional purity and quantum correlation  measures in two qubit mixed states","resumen":"We analyze and show experimental results of the conditional purity, thequantum discord and other related measures of quantum correlation in mixed two-qubit states constructed from a pair of photons in identical polarization states. The considered states are relevant for the description of spin pair states in interacting spin chains in a transverse magnetic eld. We derive clean analytical expressions for the conditional local purity and other correlation measures obtained as a result of a remote local projective measurement, which are fully veried by the experimental results. A simple exact expression for the quantum discord of these states in terms of the maximum conditional purity is also derived.","palabras_clave":"Correlaciones Cuanticas|Optica Cuantica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6780,"idioma_id":5,"titulo":"Biopolítica raciológica del control poblacional: embarazo adolescente en Argentina","resumen":"El siguiente artículo ha sido escrito en el marco del Proyecto de Investigación CYTMA2 ?Características socio-culturales del embarazo y la fecundidad adolescente en el Partido de La Matanza? dirigido por Mario Rovere y co-dirigido por Marta Susana José, a través del Departamento de Ciencias de la Salud de la Universidad Nacional de La Matanza, y realizado en el período 2015-2016. El proyecto se propone describir los aspectos socio-sanitarios relacionados con el embarazo y la fecundidad, hábitos sexuales y cuidados sanitarios de embarazadas adolescentes de 10 a 19 años.En este sentido, se indaga aquí una perspectiva de reflexión en torno a la naturaleza de la pregunta por el embarazo adolescente como problema epistemológico, desde el marco teórico referencial de la biopolítica y la bioética como áreas que permiten estructurar un abordaje filosófico sobre la temática que se plantea, en especial, a partir de los trabajos de Michel Foucault y Giorgio Agamben, interpretados desde el marco de la teoría de-colonial afro-americana.","palabras_clave":"BIOPOLÍTICA|EMBARAZO ADOLESCENTE|FECUNDIDAD|RACISMO"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6781,"idioma_id":5,"titulo":"Tratamiento de datos geotecnológicos para la obtención de información espacial en relación a Riesgos Naturales en Potrerillos, Mendoza","resumen":"ResumenLas villas cordilleranas de Potrerillos, al suroeste de la zona metropolitana de Mendoza, tienden a alojar y sostener procesos geológicos de orden interno y externos relacionados a eventos sísmicos y aluvionales, entre otros. La creciente y descontrolada urbanización que esta zona turística está teniendo, hace que gran parte de la población se desplace hacia áreas de importantes pendientes, generadoras de fenómenos relacionados con eventos pluviométricos estivales donde se activan procesos de remoción en masa, que en parte son activados por acontecimientos sísmicos, comunes en el área. Estos procesos junto a otros de menor orden, pero no así menos importantes, colocan a un núcleo de habitantes en Riesgo Geológico. Se presentan en esta oportunidad, los trabajos realizados tendientes a la elaboración de cartografía de detalle para establecer planes de mitigación, zonificación y contingencia adecuados en la zona. Se realizaron estudios tomando información de sensores OLI, ETM+ y TM del programa LANDSAT, de los programas ASTER y  SENTINEL2 a los efectos de   establecer comparaciones multitemporales para determinar vectores de crecimiento urbano. Contemporáneamente a esto, se realizaron procesamientos digitales para establecer diferencias estratigráficas, topográficas, de orientación, actitud y de anisotropías propias de deformaciones estructurales que pudieran marcar parámetros físicos para determinar zonas de peligrosidad geológica e hidrológica activa. Dentro de estos análisis digitales se incluyeron trabajos con clasificación supervisada (maximum likelihood), por árbol de decisiones (utilizando modelos digitales de elevación a partir de datos propios), density slice, componentes principales y decorrelation stretch. Los resultados aportaron una salida cartográfica RASTER de detalle, la cual, complementada con trabajos de campo, permitieron resumir información para establecer exitosamente zonas de potencial peligro sísmico e hidrológico. Se presentan en esta oportunidad los gráficos resultantes.AbstractThe Potrerillos zone, southwest of the metropolitan of Mendoza, tends to accommodate and sustain geological processes of internal and external order related to seismic and alluvial events, among others. Because of the growing and uncontrolled urbanization especially of this tourist city, much of the population is moving into areas of significant slope, generating phenomena related with summer rainfall events activating landslides. These processes together with other of lower order, but not less important, expose thousands of people to Geological Risk. The development of mapping detail to establish mitigation plans, zoning and adequate contingency in the studied área, are presented here. Studies with the base of OLI, ETM + and TM Landsat Program, STER program and SENTINEL2 sensor information were made, in order to perform, multitemporal studies to determine Urban Growth rates. Simultaneously, digital processing was performed to establish stratigraphic, topographic differences, orientation, attitude and anisotropies because of structural deformations that could make physical parameters to determine geological and hydrological active hazard areas. Supervised classification (Maximum Likelihood), decision tree (using digital elevation models from own data), density slice; Principal components and decorrelation stretch were included. The results provided a detailed cartographic output RASTER, complemented by field work, that allowed summarize information to successfully establish zones of seismic and hydrological potential hazard. The resulting graphs are presented in this opportunity","palabras_clave":"Teledetección|Imágenes|Peligro|Riesgo"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6782,"idioma_id":4,"titulo":"Differential metabolic rearrangements after cold storage are correlated with chilling injury resistance of peach fruits","resumen":"Reconfiguration of the metabolome is a key component involved in the acclimation to cold in plants; however, few studies have been devoted to the analysis of the overall metabolite changes after cold storage of fruits prior to consumption. Here, metabolite profiling of six peach varieties with differential susceptibility to develop mealiness, a chilling-injury (CI) symptom, was performed. According to metabolic content at harvest; after cold treatment; and after ripening, either following cold treatment or not; peach fruits clustered in distinct groups, depending on harvest-time, cold treatment, and ripening state. Both common and distinct metabolic responses among the six varieties were found; common changes including dramatic galactinol and raffinose rise; GABA, Asp and Phe increase; and 2-oxo-glutarate and succinate decrease. Raffinose content after long cold treatment quantitatively correlated to the degree of mealiness resistance of the different peach varieties; and thus, raffinose emerges as a candidate biomarker of this CI disorder. Xylose increase after cold treatment was found only in the susceptible genotypes, indicating a particular cell wall reconfiguration of these varieties while being cold-stored. Overall, results indicate that peach fruit differential metabolic rearrangements due to cold treatment, rather than differential metabolic priming before cold, are better related with CI resistance. The plasticity of peach fruit metabolism renders it possible to induce a diverse metabolite array after cold, which is successful, in some genotypes, to avoid CI.","palabras_clave":"CHILLING INJURY|COLD|METABOLOME|PEACH FRUIT"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6783,"idioma_id":4,"titulo":"Galectin-1 circumvents lysolecithin-induced demyelination through the modulation of microglial polarization\/phagocytosis and oligodendroglial differentiation","resumen":"Galectin-1 (Gal-1), a member of a highly conserved family of animal lectins, binds to the common disaccharide [Gal&#946;(1-4)-GlcNAc] on both N- and O-glycans decorating cell surface glycoconjugates. Current evidence supports a role for Gal-1 in the pathophysiology of multiple sclerosis (MS), one of the most prevalent chronic inflammatory diseases. Previous studies showed that Gal-1 exerts neuroprotective effects by promoting microglial deactivation in a model of autoimmune neuroinflammation and induces axonal regeneration in spinal cord injury. Seeking a model that could link demyelination, oligodendrocyte (OLG) responses and microglial activation, here we used a lysolecithin (LPC)-induced demyelination model to evaluate the ability of Gal-1 to preserve myelin without taking part in T-cell modulation. Gal-1 treatment after LPC-induced demyelination promoted a significant decrease in the demyelinated area and fostered more efficient remyelination, concomitantly with an attenuated oligodendroglial progenitor response reflecting less severe myelination damage. These results were accompanied by a decrease in the area of microglial activation with a shift toward an M2-polarized microglial phenotype and diminished astroglial activation. In vitro studies further showed that, mechanistically, Gal-1 targets activated microglia, promoting an increase in their myelin phagocytic capacity and their shift toward an M2 phenotype, and leads to oligodendroglial differentiation. Therefore, this study supports the use of Gal-1 as a potential treatment for demyelinating diseases such as MS.","palabras_clave":"GALECTIN-1|DEMYELINATION-REMIELINATION|MICROGLIA |LYSOLECITHIN |PHAGOCYTOSIS"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6784,"idioma_id":4,"titulo":"Jaw myogenesis in the monk parakeet: evidence of developmental reprogramming in the emergence of novel muscles in Psittaciformes (Aves)","resumen":"Psittaciformes have apomorphies in the muscles of the jaw that include both the adductors m.ethmomandibularis (EM) and m. pseudomasseter (PM), which are equally responsible for the generation of strong bite forces. While the EM is present in all Psittaciformes, the PM can be absent or present, and even under-developed or well-developed. The aim of this study is to identify developmental reprogramming processes by comparing the myogenesis of the jaw of the monk parakeet Myiopsitta monachus with the information available about other species of Psittaciformes.Seventeen specimens including embryos at different developmental stages, and nestlings of different ages were studied through the analysis of serial histological sections. At embryonic stage 24 (S24) the muscle precursor was observed in the first pharyngeal arch. At S27 the muscle precursor was found to be divided into lateral, intermediate and medial portions. At S31 theindependent development of the EM as a rostro-dorsal projection of the mm. pterygoidei could be observed. At S36 the individualization of all muscles was Complete. Finally, the PM was detected two days after hatching as an aponeurotic dorsal projection of the m. adductor mandibulae externus superficialis, located lateral to the arcus jugalis. Our results suggest that in M. monachus themuscles EM and PM emerge as a result of a process of heterotipy, and variations in the degree of development of the PM are associated to a heterochronic process of post-displacement, with M.monachus having an underdeveloped PM with respect to basal Psittaciformes.","palabras_clave":"EVO-DEVO|HETEROCHRONY|HETEROTIPY|MORPHOLOGICAL NOVELTIES|Myiopsitta monachus"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6785,"idioma_id":5,"titulo":"¿Ethos femenino y feminine style? El primer discurso público de dos \tpresidentes mujeres, Michelle Bachelet (Chile) y Cristina Fernández de Kirchner (Argentina","resumen":"En este trabajo, contrasto la construcción del ethos en la alocución de asunción de la presidente de Chile, Michelle Bachelet (2007) y en la de Cristina Fernández de Kirchner (2007). Indago si responde a lo que en el ámbito francés se llama ethos féminin, noción que tiene semejanza con lo que investigadores de Estados Unidos denominan feminine style;  analizo cómo usan el argumento explícito por el género y la argumentación indirecta por el género e interpreto los resultados como estrategias de legitimación.","palabras_clave":"ETHOS FEMENINO|FEMININE STYLE"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6786,"idioma_id":4,"titulo":"Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver","resumen":"AIM: To develop a simplified bioartificial liver (BAL) device prototype,suitable to use freshly and preserved liverMicroorgans (LMOs) as biological component. METHODS: The system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan® solutions. Fresh LMOs were used as controls. After preservation, LMOs were loaded into the BAL and an ammonia overload was added. To assess LMOs viability and functionality, samples were taken to determine lactate dehydrogenase (LDH) release and ammonia detoxification capacity. RESULTS: The concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan® were able to detoxify 52.9% ± 6.5% and 53.6% ± 6.0%, respectively, of the initial ammonia overload. No significant differences were found with Controls (49.3% ± 8.8%, P < 0.05). LDH release was 6.0% ± 2.3% for control LMOs, and 6.2% ± 1.7% and14.3% ± 1.1% for BG35 and ViaSpan® cold preserved LMOs, respectively (n = 6, P < 0.05). CONCLUSION: This prototype relied on a simple design and excellent performance. It?s a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.","palabras_clave":"Bioartificial liver device|Rat Liver Microorgans|Cold Preservation|BG35 preservation solution|Acute liver failure"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6787,"idioma_id":4,"titulo":"Structural and optical properties of ZnO and manganese-doped ZnO","resumen":"The structural, morphological and optical properties of manganese oxides supported on commercial ZnO, prepared by impregnation with manganese nitrate, were investigated.The nominal compositions of Mn in the samples were 5, 10, 15 and 20 %w\/w.The Xray diffraction (XRD) experiments revealed the presence of the ZnO wurtzite phase in all the samples, and ZnMnO3 was also found in the samples with 15 wt% and 20 wt% of Mn. X-ray photoelectron spectroscopy (XPS) showed the presence of Mn2+ and Mn4+ in the xMnZnO samples. The results showed that the Mn2+ ions hadsubstituted the Zn2+ ions without changing the wurtzite structure of ZnO, while Mn4+ might be assigned to the ZnMnO3 impurity phase. The optical band gap was found to be 3.3 eV for undoped ZnO samples and 3.10 eV for Mn-doped samples. The lattice defect distribution was investigated using the positron annihilation method. The results revealed that the average lifetime is a function of the Mn concentration and the formation of the ZnMnO3 phase","palabras_clave":"Mn?ZnO|Structural properties|Optical properties|Band gap energy"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6788,"idioma_id":4,"titulo":"Is anaerobic mineralizable nitrogen suitable as a soil quality\/health indicator?","resumen":"La dinámica de la materia orgánica del suelo (SOM) y especialmente la de sus Fracciones lábiles, tales como la materia orgánica particulada (POM), son muy sensibles al uso del suelo e influencian fuertemente sus servicios cosistémicos.La POM ha sido propuesta como indicador de calidad\/salud edáfica pero su determinación es tediosa y consumidora de tiempo (i.e. horas-hombre). El nitrógeno mineralizable en anaerobiosis (AN) está estrechamente relacionadocon la fracción orgánica del suelo y es muy fácilmente determinado. Por lo tanto, proponemos evaluar el AN como indicador de calidad\/salud edáfica a través de la evaluación de sus relaciones con SOM, POM, la estabilidad de los agregados (AS) y el rendimiento relativo de maíz (Zea mays L.) (RY) bajo diferentes usos agrícolas de largo plazo en Balcarce, Argentina (37º 45? 14?? S, 58º 17? 52?? W). Las muestras de suelo fueron tomadas a dos profundidades (0-5 and 5-20 cm) en otoño de 1998, 2000, 2003, 2006, 2009 y 2012, de un ensayo de larga duración de sistemas de labranza (TS, labranza convencional (CT) y siembra directa (NT)) y fertilización nitrogenada (NF, con y sin fertilización nitrogenada) sobre un complejo de Argiudoles Típico y Petrocálcico. El contenido de carbono enla SOM (SOC) y en la POM (POC), y el AN fueron determinados en todas las muestras de suelo, mientras que la AS fue determinada en otras muestras de suelo tomadas en 2006, 2009 y 2012 de la capa arable (0-20 cm). Independientemente del TS y de la NF, SOC, POC y AN disminuyeron en el tiempo bajo cultivo a 5-20 cm y a 0-20 cm. En la capa más superficial (0-5 cm) las tres variables disminuyeron sólo bajo CT. La variación de AN se relacionó con la de SOC (R2 = 0,59-0,78, P < 0.05) y especialmente la de POC (R2 = 0,80-0,85, P < 0.05). Del mismo modo, la variación del RY de maíz se relacionó mejor (R2 0,92 y 0,95 (P < 0.05) para CT y NT, respectivamente) con la de AN que con las de SOC y POC. Además, el cambio en el diámetro medio ponderado de los agregados (&#916;MWD) a 0-20 cm se relacionó aceptablemente con AN (R2 = 0,67, P < 0.05), pero la relación fue mucho mejor a 0-5 cm (R2 = 0.86, P < 0.05). Ambos coeficientes de determinación fueron más altos que aquéllos obtenidos relacionando &#916;MWD con SOC o POC. Dada la facilidad de determinación, su sensibilidad y que se relaciona con la variación de diferentes parámetros edáficos fundamentales y con el comportamiento del maíz, AN podría ser propuesto como un efectivo indicador de calidad\/salud edáfica. Sin embargo, se deberían llevar a cabo más estudios ampliando el rango de situaciones de manejo a efectos de validar los resultados obtenidos en este trabajo.","palabras_clave":"MATERIA ORGÁNICA PARTICULADA|ESTABILIDAD DE AGREGADOS|SISTEMAS DE CULTIVO|SIEMBRA DIRECTA Y LABRANZA CONVENCIONAL"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6789,"idioma_id":4,"titulo":"Modulation of antigen processing by heme-oxygenase 1. Implications on inflammation and tolerance","resumen":"Heme-Oxygenase-1 (HO-1) is an enzyme responsible for the degradation of heme that can suppress inflammation, through the production of carbon monoxide (CO). It has been shown in several experimental models that genetic and pharmacological induction of HO-1, as well as non-toxic administration of CO can reduce inflammatory diseases, such as endotoxic shock, type-1 diabetes and graft rejection. Recently, it was shown that the HO-1\/CO system can alter the function of antigen presenting cells (APCs) and reduce T cell priming, which can be beneficial during immune-driven inflammatory diseases. The molecular mechanisms by which the HO-1 and CO reduce both APCs- and T cell-driven immunity are just beginning to be elucidated. In this article we discuss recent findings related to the immune regulatory capacity of HO-1 and CO at the level of recognition of pathogen-associated molecular patterns (PAMPs) and T cell priming by APCs. Finally, we propose a possible regulatory role for HO-1 and CO over the recently described mitochondria-dependent immunity. These concepts could contribute to the design of new therapeutic tools for inflammation-based diseases. This article is protected by copyright. All rights reserved.","palabras_clave":"hemeoxygenase|immunity|dendritic cells|antigen presentation"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6790,"idioma_id":5,"titulo":"Cambios en la comunidad de arañas (Arachnida: Araneae) en períodos de barbecho y de cultivos de soja en el Norte de santa Fe, Argentina","resumen":"Se estudió los cambios en la comunidad de arañas en áreas cultivadas de soja y de barbecho en el norte de la provincia de Santa Fe","palabras_clave":"Biodiversidad|Aracnología|Ecología"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6791,"idioma_id":4,"titulo":"Performance  of  ZnSe(Te)  as  fiberoptic  dosimetry detector","resumen":"Fiberoptic dosimetry (FOD) is an experimental technique suitable for in-vivo, real time dosimetry in radiotherapy treatments. FOD relies on using a small scintillator coupled to one end of a long optical fiber. The scintillator is placed at the point where the dose rate is to be determined whereas a lightdetector at the other end of the fiber measures the intensity of the radioluminescence emitted by the scintillator. One of the problems hampering the straightforward application of this technique in clinics is the presence of Cherenkov radiation generated in the fiber by the ionizing radiation, which adds to the scintillating light and introduces a bias in the dose measurement. Since Cherenkov radiation is more important in short wavelength range of the visible spectrum, using red-emitting scintillators as FOD detectors permits to reduce the Cherenkov contribution by using optical filters. In this work, the performance of red-emitting tellurium-doped zinc selenide crystal as FOD detector is evaluated and compared to the response of an ion-chamber.","palabras_clave":"FIBER OPTIC DOSIMETRY|REAL TIME DOSIMETRY|TELLERIUM DOPED ZINC SELENIDE|ZnSe(Te)"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6792,"idioma_id":5,"titulo":"Prescripción de medicamentos oncológicos en un servicio de oncología público de Rosario: adecuación a guías de práctica clínica","resumen":"Objetivo: Evaluar la prescripción de medicamentos oncológicosde la Red de Salud Pública Municipal de Rosario según su adecuaciónlas guías de práctica clínica.Método: Estudio farmacoepidemiológico descriptivo en pacientesadultos en un Servicio Oncológico. Enero-junio 2012.Se evaluó la adecuación de las prescripciones a las guías depráctica clínica de referencia.Resultados: El 51,8 % de los diagnósticos tuvo al menos unmedicamento prescripto que no coincidía con lo recomendadopor al menos una de las guías consideradas. Las prescripcionesde doxorrubicina e ifosfamida no coincidieron con lo recomendadopor ninguna guía. El 5,4% de las prescripciones no estabanconsideradas en las guías locales, el 7,7% no lo estabanen las nacionales y, respecto de las internacionales, el 4,2 %no estaban consideradas en la European Society for MedicalOncology, el 2,3% por el American Cancer Society y solo el1,9% por la National Comprehensive Cancer Network.Conclusiones: La prescripción de oncológicos se adecúa más alas guías internacionales. Podría deberse a que no existe aúnuna definición estándar en el manejo de las patologías tumoralespor parte del Estado Nacional.","palabras_clave":"política de medicamentos|medicamentos oncologicos|farmacoepidemiologia|guias de practica clinica"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6793,"idioma_id":4,"titulo":"Effect of xanthan gum on the rheological behavior and microstructure of sodium caseinate acid gels","resumen":"The aim of this work was to study the effect of xanthan gum (XG) on the gelation process of bovine sodium caseinate (NaCAS) induced by acidification with glucono-&#948;-lactone (GDL) and on the mixed acid gel microstructure. Before GDL addition, segregative phase separation was observed in all the NaCAS-XG mixtures evaluated. The gelation process was analyzed by using a fractional factorial experimental design. The images of the microstructure of the mixed acid gels were obtained by conventional optical microscopy and the mean diameter of the interstices was determined. Both the elastic character and the microstructure of the gels depended on the concentrations of XG added. As XG concentration increased, the kinetics of the gelation process was modified and the degree of compactness and elasticity component of the gel network increased. The microstructure of gels depends on the balance among thermodynamic incompatibility, protein gelation and NaCAS-XG interactions.","palabras_clave":"Xanthan Gum |Microstructure|Rheology|Acid Gels|Sodium Caseinate"},{"tipo_produccion_id":1,"anio_publica":2016,"producto_id":6794,"idioma_id":5,"titulo":"Inscripciones pos-soviéticas en la literatura cubana actual. Futuros negativos y desfiguraciones de lo humano en El imperio de Oblómov de Carlos Aguilera","resumen":"En una gran parte de la literatura cubana escrita por la generación de autores que comenzó a publicar alrededor de 1989 y hasta la actualidad puede leerse la insistencia de varios significantes vinculados con imaginarios soviéticos: rusos, de Europa oriental, chinos. Rafael Rojas (2009) ha señalado los vestigios exotistas que parece haber en dicho ejercicio, mientras que otros críticos, como Jacqueline Loss (2012) o Damaris Puñales Alpízar (2013), entienden el retorno como una pulsión identitaria en la diáspora, una comunidad de sentimiento soviético cubana. En el siguiente artículo nos interesa abordar la hipótesis que sostiene que una de las claves para leer la inscripción del archivo soviético se relacionan con la dislocación y la desfiguración